LOCUS       DAVMEI010000001        66051 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVMEI010000001 DAVMEI010000000
VERSION     DAVMEI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.001"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(21..956)
                     /locus_tag="VYQ24_000001"
     CDS             complement(21..956)
                     /locus_tag="VYQ24_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1967200.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLQQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(953..1813)
                     /locus_tag="VYQ24_000002"
     CDS             complement(953..1813)
                     /locus_tag="VYQ24_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1967201.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKDKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            1921..4083
                     /locus_tag="VYQ24_000003"
     CDS             1921..4083
                     /locus_tag="VYQ24_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1967202.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4175..5434
                     /locus_tag="VYQ24_000004"
     CDS             4175..5434
                     /locus_tag="VYQ24_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1967203.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNAYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTSFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5448..6314
                     /locus_tag="VYQ24_000005"
     CDS             5448..6314
                     /locus_tag="VYQ24_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1967204.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6328..7161
                     /locus_tag="VYQ24_000006"
     CDS             6328..7161
                     /locus_tag="VYQ24_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1967205.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     VFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7162..7242
                     /locus_tag="VYQ24_000007"
                     /pseudo
     CDS             7162..7242
                     /locus_tag="VYQ24_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7519..8961
                     /gene="gtfA"
                     /locus_tag="VYQ24_000008"
     CDS             7519..8961
                     /gene="gtfA"
                     /locus_tag="VYQ24_000008"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1967206.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKSLLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9099..10292
                     /locus_tag="VYQ24_000009"
     CDS             9099..10292
                     /locus_tag="VYQ24_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1967207.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVFCLYIIS
                     LIFTGDMKSILQKMGEHSMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10757..12103
                     /locus_tag="VYQ24_000010"
     CDS             10757..12103
                     /locus_tag="VYQ24_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV1967208.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     LISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDIISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFLKHLAGGDFQTLTIKRFHHLFLH
                     VVGNCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            12685..14664
                     /locus_tag="VYQ24_000011"
     CDS             12685..14664
                     /locus_tag="VYQ24_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967209.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14731..16227
                     /locus_tag="VYQ24_000012"
     CDS             14731..16227
                     /locus_tag="VYQ24_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967210.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            16227..17153
                     /locus_tag="VYQ24_000013"
     CDS             16227..17153
                     /locus_tag="VYQ24_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967211.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            17162..18229
                     /locus_tag="VYQ24_000014"
     CDS             17162..18229
                     /locus_tag="VYQ24_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967212.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGELYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            18240..19166
                     /locus_tag="VYQ24_000015"
     CDS             18240..19166
                     /locus_tag="VYQ24_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967213.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            19656..20387
                     /locus_tag="VYQ24_000016"
     CDS             19656..20387
                     /locus_tag="VYQ24_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1967214.1"
                     /translation="MGILLTSLISLHQLKADKKRDVFRIGISQFITHQSLDATREGFV
                     DELAKQGYVEGENIEIDLQNAQGEQRNLKTISQQLAESSDVVLAIARPSAQSEPNSVV
                     QKDEAKRLLKEKGFTVVEKTILDSNNVKAAAESLMAEVDMVFVPTDNIISLTMETVKQ
                     VSIKHKVPVFGGSTEMIAVGGLYNYGTNYEELGRQTARMLIHVLKGEKPENIAVELPE
                     KLELHTNQEMADALGIDISKLEGKE"
     gene            20399..21292
                     /locus_tag="VYQ24_000017"
     CDS             20399..21292
                     /locus_tag="VYQ24_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967215.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGTVFSLSLACFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIVEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLAL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            21305..22108
                     /locus_tag="VYQ24_000018"
     CDS             21305..22108
                     /locus_tag="VYQ24_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967216.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMTDLMKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(22141..22851)
                     /gene="treR"
                     /locus_tag="VYQ24_000019"
     CDS             complement(22141..22851)
                     /gene="treR"
                     /locus_tag="VYQ24_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1967217.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            23036..25003
                     /gene="treP"
                     /locus_tag="VYQ24_000020"
     CDS             23036..25003
                     /gene="treP"
                     /locus_tag="VYQ24_000020"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1967218.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVSHELVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETLVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            25181..26806
                     /gene="treC"
                     /locus_tag="VYQ24_000021"
     CDS             25181..26806
                     /gene="treC"
                     /locus_tag="VYQ24_000021"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1967219.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            27062..27310
                     /locus_tag="VYQ24_000022"
     CDS             27062..27310
                     /locus_tag="VYQ24_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1967220.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            27517..28311
                     /gene="racE"
                     /locus_tag="VYQ24_000023"
     CDS             27517..28311
                     /gene="racE"
                     /locus_tag="VYQ24_000023"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1967221.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            28308..29279
                     /locus_tag="VYQ24_000024"
     CDS             28308..29279
                     /locus_tag="VYQ24_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1967222.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            29255..29776
                     /locus_tag="VYQ24_000025"
     CDS             29255..29776
                     /locus_tag="VYQ24_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1967223.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            29773..30234
                     /locus_tag="VYQ24_000026"
     CDS             29773..30234
                     /locus_tag="VYQ24_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1967224.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            30225..30965
                     /gene="xerD"
                     /locus_tag="VYQ24_000027"
     CDS             30225..30965
                     /gene="xerD"
                     /locus_tag="VYQ24_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1967225.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            30965..31693
                     /locus_tag="VYQ24_000028"
     CDS             30965..31693
                     /locus_tag="VYQ24_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1967226.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            31712..32281
                     /locus_tag="VYQ24_000029"
     CDS             31712..32281
                     /locus_tag="VYQ24_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1967227.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            32268..32990
                     /locus_tag="VYQ24_000030"
     CDS             32268..32990
                     /locus_tag="VYQ24_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1967228.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            32990..33232
                     /gene="yidD"
                     /locus_tag="VYQ24_000031"
     CDS             32990..33232
                     /gene="yidD"
                     /locus_tag="VYQ24_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1967229.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFDLTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(33457..34422)
                     /locus_tag="VYQ24_000032"
     CDS             complement(33457..34422)
                     /locus_tag="VYQ24_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967230.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34518..35270)
                     /locus_tag="VYQ24_000033"
     CDS             complement(34518..35270)
                     /locus_tag="VYQ24_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967231.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(35267..36223)
                     /locus_tag="VYQ24_000034"
     CDS             complement(35267..36223)
                     /locus_tag="VYQ24_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1967232.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYILLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILIGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(36213..37172)
                     /locus_tag="VYQ24_000035"
     CDS             complement(36213..37172)
                     /locus_tag="VYQ24_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967233.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(37469..37579)
                     /locus_tag="VYQ24_000036"
     CDS             complement(37469..37579)
                     /locus_tag="VYQ24_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEV1967234.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            complement(37595..38296)
                     /gene="galE"
                     /locus_tag="VYQ24_000037"
     CDS             complement(37595..38296)
                     /gene="galE"
                     /locus_tag="VYQ24_000037"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1967235.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            38697..39761
                     /gene="pepA"
                     /locus_tag="VYQ24_000038"
     CDS             38697..39761
                     /gene="pepA"
                     /locus_tag="VYQ24_000038"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1967236.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            39783..40079
                     /locus_tag="VYQ24_000039"
     CDS             39783..40079
                     /locus_tag="VYQ24_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1967237.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            40761..41204
                     /locus_tag="VYQ24_000040"
     CDS             40761..41204
                     /locus_tag="VYQ24_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1967238.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            41208..41972
                     /locus_tag="VYQ24_000041"
     CDS             41208..41972
                     /locus_tag="VYQ24_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1967239.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            41987..42715
                     /locus_tag="VYQ24_000042"
     CDS             41987..42715
                     /locus_tag="VYQ24_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967240.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIENGEFMVLVGPSGSGKTTMI
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLTTGYVLQAIALFPNLTVEENIALIPEM
                     KGWTKEEIAQKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            42708..44228
                     /locus_tag="VYQ24_000043"
     CDS             42708..44228
                     /locus_tag="VYQ24_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1967241.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALMAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            44573..45379
                     /locus_tag="VYQ24_000044"
     CDS             44573..45379
                     /locus_tag="VYQ24_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1967242.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(45545..46081)
                     /locus_tag="VYQ24_000045"
     CDS             complement(45545..46081)
                     /locus_tag="VYQ24_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1967243.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            46214..47104
                     /locus_tag="VYQ24_000046"
     CDS             46214..47104
                     /locus_tag="VYQ24_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1967244.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            47348..47701
                     /locus_tag="VYQ24_000047"
     CDS             47348..47701
                     /locus_tag="VYQ24_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1967245.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            47702..48739
                     /locus_tag="VYQ24_000048"
     CDS             47702..48739
                     /locus_tag="VYQ24_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1967246.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIIIE"
     gene            complement(48786..49793)
                     /gene="galR"
                     /locus_tag="VYQ24_000049"
     CDS             complement(48786..49793)
                     /gene="galR"
                     /locus_tag="VYQ24_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1967247.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALKEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTKLTLRESTLNQ
                     E"
     gene            49957..51135
                     /locus_tag="VYQ24_000050"
     CDS             49957..51135
                     /locus_tag="VYQ24_000050"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1967248.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPATISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVSLDIQTLGELDEWAVDQYSYLIKDENRLKRVR
                     HAVLENQRTLKAQVALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            51154..52635
                     /locus_tag="VYQ24_000051"
     CDS             51154..52635
                     /locus_tag="VYQ24_000051"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1967249.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPYPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            52705..53109
                     /locus_tag="VYQ24_000052"
     CDS             52705..53109
                     /locus_tag="VYQ24_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1967250.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            53290..54054
                     /locus_tag="VYQ24_000053"
     CDS             53290..54054
                     /locus_tag="VYQ24_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1967251.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNDPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYWKL"
     gene            54051..54512
                     /locus_tag="VYQ24_000054"
     CDS             54051..54512
                     /locus_tag="VYQ24_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1967252.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(54563..55825)
                     /locus_tag="VYQ24_000055"
     CDS             complement(54563..55825)
                     /locus_tag="VYQ24_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1967253.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIAMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(55825..56406)
                     /locus_tag="VYQ24_000056"
     CDS             complement(55825..56406)
                     /locus_tag="VYQ24_000056"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1967254.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(56806..56901)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(57284..57412)
                     /locus_tag="VYQ24_000057"
     CDS             complement(57284..57412)
                     /locus_tag="VYQ24_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV1967255.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHEFAVLDPNGYFLRFSE"
     gene            complement(57523..57666)
                     /locus_tag="VYQ24_000058"
     CDS             complement(57523..57666)
                     /locus_tag="VYQ24_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1967256.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            complement(57678..58505)
                     /gene="xth"
                     /locus_tag="VYQ24_000059"
     CDS             complement(57678..58505)
                     /gene="xth"
                     /locus_tag="VYQ24_000059"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1967257.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            58639..59095
                     /locus_tag="VYQ24_000060"
                     /pseudo
     CDS             58639..59095
                     /locus_tag="VYQ24_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            59248..59622
                     /locus_tag="VYQ24_000061"
     CDS             59248..59622
                     /locus_tag="VYQ24_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1967258.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYEKEKP
                     GVLYSSKISSNRVNVALPYVGY"
     gene            59750..61495
                     /locus_tag="VYQ24_000062"
     CDS             59750..61495
                     /locus_tag="VYQ24_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967259.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            61485..63236
                     /locus_tag="VYQ24_000063"
     CDS             61485..63236
                     /locus_tag="VYQ24_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967260.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMADTL"
     gene            63409..64101
                     /locus_tag="VYQ24_000064"
     CDS             63409..64101
                     /locus_tag="VYQ24_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1967261.1"
                     /translation="MLKWEDLPVEMKSSEVESYYHLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            64144..65370
                     /locus_tag="VYQ24_000065"
     CDS             64144..65370
                     /locus_tag="VYQ24_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1967262.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
BASE COUNT        19098 a        12362 c        14841 g        19750 t
ORIGIN      
        1 tatattataa ttgtgttatt ttacttccaa ctattgagag aaatttcgcc actatttagc
       61 cagatttctt ttccgttatc acattgaact aaaagttttc cattttctga gatgtcttta
      121 gcaagtccct tgtagtcttt ttgctgtagt gtgaaagtga cttcttttcc tagaatgaat
      181 gactgttttt tgtataggta taatagctct tctgctggtg tttcgaagaa agcacgccag
      241 atttctatga tcaattcatt ccttgttata ggagctgtag ctttaaataa gctggcagct
      301 ttttctttta attcctgagg gaagtcttta atagtgaaat tgatacctac tccaataatg
      361 atatctgtga ctaagccagt ttctacagag gtcattgctt cagtaaggat tcctccaatt
      421 ttatgattgt ttagatagat atcattgacc cattttatgt cgacatctat taaagttagg
      481 ttcttaatag ctttgtagac agctccagct acaagtagtg tgtaggatgg taatttgtca
      541 taggggagat ttggtttaag atggagtgtc atataaatac caccttgtgg tgagtagaag
      601 gaacgttgaa aacgtcctcg gcctgctgtt tgataggaag ctagatagag ggtatttgct
      661 tcatggccta aatcaattgc ttcttttgca tctagttgtg ttgattttgt ttcgggttta
      721 aagctgactt taattggaag attttcttct agaatctctg gaagaataag gtcaccattc
      781 attagtttat atcctctatt tttgatacta tcaatttcaa tgccttcttg ttctagtcgc
      841 ttgatagctt tccaaattga tgttcggctt agggatagtt tttctgcgat tttttctccg
      901 ctgatatagt cggtttcttt agataggatt tggtagacag cttggtagga tttcataatg
      961 ttgcctttct cactagttgg tattgagagt attcttttct tgtatgactt ggagtctgat
     1021 taaagtattg tttataagct ttcgaaaaat ggagtggatc tgaaaaacct accgagtatg
     1081 caattacctt gatggactct tgggtatttt cgagaagttg tctagctcgg tgcattcgaa
     1141 cgtagagtag gtattctttg ggtgataagg tattaaattc tttgaatacg cttgataagt
     1201 agcttctgtg aacggatagt tcttttgcta aatcttgaat tgtaagtgat tgaggatagt
     1261 ggctatcaat taatcgtttg cattcaagat agagttggtg ggttgatgaa atattctttt
     1321 ttttctgatt gggagcaata gttcccagat gaaacatcag ttcatgaagt tgtcccatga
     1381 tatggagttg agctaattca cttgattttg taatctgagc gaagcggaca atgtctgaga
     1441 tgagttttgc agtagtctgg gtatgacaag tttcagattg gatgagatag gattgatcag
     1501 aaatttgaga aagagcaaaa taatcagggg atttccctcc agtgattcct aaccagtagt
     1561 aggcccaagg ttctttacta tctgcttgat aaaaggttag ttcctctggt tttaatagaa
     1621 agaaatctcc ttcttttaaa tcaacaattt tatccttgta atgaaatttt ccttgtcctt
     1681 tagtaatgta atgtaggacg tatgtatcac gaatggctgg accaaaagag taattaggtg
     1741 tgcattcctc atatccataa aagcttaggg caaggtcgat tgttccagtc tggtattctg
     1801 aaaaaactaa catgtgctac ctcctaccta acattttacc atattcttga gacatttttc
     1861 tattttggaa agcgatttca ggtgataaaa tataatcaat aaagtgatga ggtgaagtaa
     1921 atgggagtta ggatagagaa taatctattt tatgttgaga gtaaaaatct aagtttgatt
     1981 attgaaaatc gaaatggcta cttacttttg aaacatttag gaaagactat taagaactat
     2041 aaaggttcca atagtgttta tgaacgagac catgcctttt caggaaatcc aacggctact
     2101 aatcgaacct ttagtttaga tactcagcga cagatttttg gacaacatgg cttaggagat
     2161 tttaggaaac caactataca ggttcagcat agtgtaactg aagtaacaga ctttcgattt
     2221 gtagaagcaa agattttaaa aggtcagaat ggtccacagg gcttaccttc tccacatagc
     2281 atggacgata cagagactct tgtcttaatg ttagaagatt ctaaggctca acttagtctg
     2341 actttgtatt atactacttt taataatgat gcgactatta ctagctacag taaattagat
     2401 aataatagta atcaggaagt tgtcatccat aaagattttt cttttatggc tgattttcca
     2461 gctgcagatt atgaaatagc aactctgcag ggtgcttatg ctcgtgaaaa gactgttaga
     2521 cgtcaacagg tagaacaagg aatcttttcg attagttcaa accgaggtgc ttctggtcat
     2581 gctcaaacac cagctcttct actatgcgaa caaggagtca cagaggatgc tgggaatgtg
     2641 tttgctattc aactaatgta tagtggcaac tttgaagctt ttgttcaaaa aaatcaattg
     2701 aatgaagttc gggtggctat tggcattaat ccagaaaact tttcttggaa gttagcccct
     2761 gaggaatact ttgaaacacc ggtagcttta gtgactcatt cagatcaggg attaactggt
     2821 attagtcatg aaagtcagaa ttttgtactg aagcacatta tgctaagtga attttctaaa
     2881 aaagaacgtc caattctaat caataactgg gaagctactt actttgactt tcagagagaa
     2941 aaactgttag agttagcaga tgaagctaag aaagttggca ttgaactttt tgtattagat
     3001 gatggttggt ttggcaatcg ttttgatgat aatcgtgctt taggtgattg ggttgttaat
     3061 gaggaaaaac tgggtggaag tctagaaagt ctgatttcag ctatccatga aagaggtttg
     3121 cagtttggac tttggttaga acccgaaatg atttctgtag atagtgattt gtatcgtcaa
     3181 catcctgact gggctattca ggttcctgat tatgagcata cttattctcg gaatcaatta
     3241 gtacttaatc ttgccaatcc tcaggtagta gaatacttga aaagtgtctt agatcaactc
     3301 ctatcttatc atgagattga ttacattaaa tgggatatga accgcaatat cactaagcta
     3361 gggaatggat taacttatct agagacacag atgcaatctc atcagtacat gctggggctt
     3421 tacgaactcg tttcttatct gacagagaag cacagccata ttctctttga gtcctgctct
     3481 ggtggtggtg gacgaaatga tcttggtatg atacgctatt tcccacaagt ctgggctagt
     3541 gataatactg atgccattgc acgtttacca attcaatacg gttcatccta tctctatcca
     3601 accatttcta tgggggctca tgtgtcagca gtaccgaatc atcagatggg acgaatgaca
     3661 ccattagaaa cacgtggcca tgtagcaatg atgggaaatt tgggctatga gcttgatttg
     3721 acaaatttat cagatgaaga gaaagctacg attgctaatc aggtgaactt gtataaagaa
     3781 ttacgaccag tagttcagtt aggacagcag tatagactaa ttaatcctga tactgcatcc
     3841 aatgaagcag ctgtacaatt taattacgga aatcaaacga ttgtaaccta cgttcgcgtt
     3901 ttatctgttg tagagaccat ggaaacaact ttaaaattaa aagatttgga tgaagaggga
     3961 ctatataaat tacaggaaaa tggcgaagtt tactcaggtg cagaactaat gtatgcgggc
     4021 ttaactgtta tcttatccca aggagatttt ttgagtagac agtatatttt tagaaaacta
     4081 tgatatgtac tgaccccaaa agttagattt attctgtcta acttttgggg tgcagttcaa
     4141 taagggtgta taaatttata aaggaggctt tctaatggaa tggtataaaa aaatcggact
     4201 tcttgcaact acaggtttag ctttgtttgg gctcggcgct tgctccaact atggtaaatc
     4261 tgcggatggc acagtgacca tcgagtattt caaccagaaa aaagaaatga ccaaaacctt
     4321 ggaagaaatc actcgtgatt ttgagaagga aaaccctaag atcaaggtca aagtcgtcaa
     4381 tgtaccaaat gctggtgaag tattgaagac acgcgttctc gcaggagatg tgcctgatgt
     4441 ggtcaatatt tacccacagt ccatcgaact gcaagaatgg gcaaaagcag gtgtttttga
     4501 agatttgagc aacaaagact acctgaaacg cgtgaaaaat gcctacgctg aaaaatatgc
     4561 tgtaaacgaa aaagtttata acgttccttt tacagctaat gcttatggaa tttactacaa
     4621 caaagataaa ttcgaagaac tgggcttgaa ggttcctgaa acctgggatg aatttgaaca
     4681 gttagtcaaa gatatcgttg ctaaaggaca aacatcattt ggaattgcag gtgcagatgc
     4741 ttggacactc aatggttaca atcaattagc ctttgcgaca gcaacaggtg gaggaaaaga
     4801 agcaaatcaa taccttcgtt attctcaacc aaatgccatt aaattgtcgg atccgattat
     4861 gaaagatgat atcaaggtca tggacatcct tcgcatcaat ggctctaagc aaaagaactg
     4921 ggaaggtgct ggctataccg atgttatcgg agccttcgca cgtggggatg tcctcatgac
     4981 accaaatggg tcttgggcga tcacagcgat taatgaacaa aaaccgaact ttaagattgg
     5041 aaccttcatg attccaggaa aagaaaaagg acaaagctta accgttggtg cgggagactt
     5101 ggcatggtct atctcagcca ccaccaaaca tccaaaagaa gccaatgcct ttgtggaata
     5161 tatgacccgt ccagaagtca tgcaaaaata ctacgatgtg gacggatctc caacagcgat
     5221 cgaaggggtc aaacaagcag gagaagattc accgcttgct ggtatgaccg aatatgcctt
     5281 tacggatcgt cacttggtct ggttgcaaca atactggacc agtgaagcag acttccatac
     5341 cttgaccatg aactatgtct tgaccggtga taaacaaggc atggtcaatg atttgaatgc
     5401 cttctttaac ccgatgaaag cggatgtgga ttaggagtag agagactatg aaaaaagtat
     5461 tacaaaaata ttgggcatgg gcttttgtgg tcatccccct cttgttacaa gcaattttct
     5521 tctatgtgcc gatgtttcaa ggagcctttt acagttttac caactggaca ggattgactt
     5581 acaactacaa atttgtcggc ttaaacaact ttaagctcct cttcatggat ccaaaattca
     5641 tgaatgcgat tggctttacc gcaatcatta cgattgccat ggtggttggt gagattgcac
     5701 tcgggatctt cattgcgcgt gtcttgaact ctaaaatcaa aggccaaacc ttcttccgtg
     5761 cttggttctt cttcccagct gttttatctg gtttgacagt ggctttgatc ttcaagcaag
     5821 tcttcaacta cggtcttcca gcgattggga atgcccttca tattgaattt ctccaaacca
     5881 gtcttttagg gactaagtgg ggagcaatct ttgcggctgt ctttgtcctt ctttggcaag
     5941 gggtggctat gcccatcatc atcttcctag ctggtttgca atctattcca actgagatta
     6001 cagaggcagc aaggattgat ggtgcgacta gcaagcaagt tttctggaac attgaattgc
     6061 cttacttgct accaagtgtc tctatggtct ttatcctagc cctaaaaggt gggctgactg
     6121 cctttgacca agtctttgcc atgaccggtg gtggtccaaa caatgccaca acctcacttg
     6181 ggctcttggt ttataactat gcctttaaaa acaaccaatt cggttatgcc aatgccattg
     6241 ccgtaatctt gttcctctta attgtagtga tttcgatcat ccaattgaga gtatctaaga
     6301 aatttgaaat ttaagaggag aagcatgatg aaacaagatg aaagaaaagc cctgattggc
     6361 aaatacattc tattgattct aggatcggtt ctgattttag tgccgctcct tgctaccctc
     6421 tttagttcct tcaaacccac taaggatatt gtagataatt tctttggctt tccaaccaac
     6481 ttcacatggg acaactttag ccgtctctta gctgatggga ttggaggcta ttattggaac
     6541 tctgtcgtca tcactgtctt gtctttactt gcagtaatga tctttatccc tatggcagcc
     6601 tactccatcg ctcgcaatat gagtaaaaga aaagtcttta ccatcatgta taccctctta
     6661 atcctcggaa tcttcgtacc tttccaagtc atcatgattc cgattacggt tatgatgagt
     6721 aaactcggtt tggctaatac ctttggtttg atcttgctct acttgaccta tgcgattcca
     6781 cagaccctct ttctctatgt tggctatatc aaaatctcga ttccagaaag tctggatgaa
     6841 gcagcagaga tcgatggggc taatcaattt acaacctatt tccgcatcat cttcccaatg
     6901 atgaaaccga tgcatgcgac aaccatgatc atcaatgccc tttggttctg gaatgacttc
     6961 atgttgccac tccttgtctt gaaccgggat tccaaaatgt ggactctgcc tttgttccaa
     7021 tacaactacg caggccaata tttcaacgac tacggaccaa gctttgcctc ttacgtggtc
     7081 ggcattatca gtatcaccat tgtctatctc ttcttccaac gccatatcat ttcaggaatg
     7141 agcaacgggg cagtgaagta atactcaatg aaaatcaaag agcaaactag gaagctagcc
     7201 gcaggttgct caaaacactg ttttgaggtt gtggatgaaa ctgacgaagt cagtaaccat
     7261 acctacggta aggtgaagct gacgtggttt gaagagattt tcgaagagta taagaagagc
     7321 tcatggaatc tatagtcttt gacacagagg aagagttcag cttgttgata attctatgta
     7381 gaaaacaaga aagtagtact ttgtttatca cagtctatat tagtacgagt ttgatgataa
     7441 atagagattt aaaatgaatt tgatttacga agccagatca tatgagactt ggcttcaatt
     7501 agaaaaagga gagtaatgat gccgattcaa aataaaacca tgttgattac ctattctgat
     7561 agccttggaa ataatcttaa agacttatat gataatttgg aagagcattt tggagatgct
     7621 attggaggag ttcacctttt accatttttc ccatcaacag gtgatcgtgg atttgcgcca
     7681 gttgactacg acgaagtgga ctcagctttt ggtgattggg aggatgttaa gcgtttaggt
     7741 gagaaatatt atcttatgtt tgattttatg attaatcata tttctcgtca atccaagtat
     7801 tataaggact atcaagaaaa acatgaagcc agtgaattta aagctctctt tttaaactgg
     7861 gataagtttt ggccagaaaa ccgtccgaca cagtctgatg tagatttaat ttacaagcgt
     7921 aaggatcgtg caccaaagca agagattgtg tttgaagatg gttcagtgga acatttgtgg
     7981 aatacctttg gtgaggagca gattgatctt gatgtgacca aagaagtaac tatggaattt
     8041 atccgtaaga ccattcagca cttggcaagt aatgggtgtg atttgattcg tctagatgcc
     8101 tttgcttatg cagtgaagaa attggatact aatgatttct ttgtggaacc agatatttgg
     8161 gatttattgg acaaagttcg agatatcgct gctgagtatg ggacagagct tttacctgag
     8221 attcatgaac actattcgat tcagtttaaa atagcagacc atgattacta tgtttatgat
     8281 tttgctcttc caatggtgac actttatact ctttacagtt ccagaacaga gcgtttggct
     8341 aagtggttaa agatgagccc gatgaagcaa tttacgacgc tagataccca tgatgggatt
     8401 ggagtagtag atgtcaagga tatcctgacc gatgaggaga ttgactatgc ttcaaatgaa
     8461 ctctataagg ttggagccaa tgtcaaacgt aagtactcta gtgccgagta taacaactta
     8521 gatatctacc aaatcaattc aacctactat tcagcgcttg gagatgatga tgtcaagtat
     8581 tttctcgctc gtctaattca agcttttgcc ccaggtattc ctcaggttta ctatgtgggt
     8641 ctattagcag gcaagaatga cttgaaatta ttagaagaaa ctaaagaagg tcgaaatatt
     8701 aatcgtcatt actatagcaa cgaggaaata gcaaaagaag tgcaacgacc tgttgtgaag
     8761 tcccttctca atctattttc tttccgtaac cgttcagaag cctttgatct agaagggact
     8821 actgagatag agacaccaac agctcacagc attgtaatca aacgtcaaaa taaagataag
     8881 tccgtaacag cagtagcaga aattgacttg caaaatcaga cttatcgggt aattgagaat
     8941 ggagttgaag taacattttg aagccttgat atggtggata aaatagggtt tttattttgg
     9001 aaaacgtttc ctttgttttc aaattgctaa aaaagtggta caatatagga tagcttacta
     9061 ttatctgaat cagctgattt ggagagaaag gattcatttt gaaatcaata ggctttattg
     9121 aaaagctgaa ggggttgtct agtaaagagc tgattttatt gggaattatc ctgagtatct
     9181 ttttaccctt ttatcttttt gtagttgtat tctgtttata tattatcagt ctgattttta
     9241 caggagacat gaaaagtatt cttcagaaaa tgggggagca ttcgatgctg cttctttttc
     9301 ttggctatag tactgttata tccattcttg cacaaaattg gatgggtctt gtggcttcag
     9361 taggaatgtt tctatttact attttctttt tgcactatca gtcgatttta tcccataaat
     9421 tctttcgatt gattttgcag ttcgtcttgt ttggtagtgt cttgtcagct gcttttgcca
     9481 gtttagaaca tttccaaatt gtgaagaaat ttaattatgc ttttctttcc cccaatatgc
     9541 aggtgtggca tcagaaccgg gcagaagtga ccttctttaa tcctaattat tatggaatta
     9601 tttgttgttt ctgtattatg attgctttct atctgtttac aacgaccaag ttgaattggt
     9661 tgaaagtatt ctgtgtgatt gcaggctttg ttaatctctt tggtttgaac tttactcaaa
     9721 atcgaactgc ctttcctgct attatcgctg gagcaattat ctatctcttt acgactatta
     9781 aaaactggaa ggccttttgg cttagtattg gggtcttcgc gattggtttg agtttcctct
     9841 tttctagtga tttgggagtt cgaatgggta ctttagactc ttctatggaa gaacgcattt
     9901 ctatctggga tgctgggatg gccttgttta agcaaaatcc tttttggggt gaagggccat
     9961 tgacctatat gcactcttat cctcggatac atgctcctta tcatgaacat gcccacagtc
    10021 tttatattga tacgattctg agttacggaa ttgtgggtac cattttatta gttctgtctt
    10081 ctgttgctcc tgttcgcttg atgatggata tgagtcagga gtcggggaaa cgtccgatta
    10141 tcggccttta tctatctttc cttacagtgg ttgctgtgca cggaattttt gacttggctc
    10201 tcttctggat tcagtcaggc tttattttct tgctagttat gtgcagtatt ccattggagc
    10261 atcgaatgtt ggtatcggac atgacggatt aagttttata agtttgaaat cttctacctt
    10321 gggtaggaga ttttttatac gatgggcatg tcgtgcatct gaggtgtaag tcctctgtag
    10381 ggatccacta ccggtgaata caatagcgct ccccaagcct gactatcgtg aggtagcgga
    10441 ttaaaatggt ctggggatag atcgttttaa gtctgacgca ggaaataaga attgtcagag
    10501 aaagcaatag tgaaatcgta ggctatgaat agaaacgtga tagcgaggag catatagtgg
    10561 ataaggtagc gagaaatttt aaagtataaa accccaatta ttcaccccta atttaaaaac
    10621 catccagaat ccttgcctta gcttagatcc tggatggttt cttttttcac ccaatgggtg
    10681 ttttttacta gacaaaaaag agtttcccct ttatggtata agtgtagaaa aaaacacaaa
    10741 aagaaaggaa actcacatga acagtttacc aaatcatcac ttccaaaaca agtcttttta
    10801 ccaactatct ttcgatggag gtcatttaac ccagtatggt ggtcttatct tttttcagga
    10861 acttttttcc cagttgaaac taaaagagct gatttctaag tatttagtaa cgaatgacca
    10921 acgccgctac tgtcgttatt cggattcaga tatccttgtc cagttcctct ttcaactgtt
    10981 aacaggctat ggaacggact atgcttgtaa agaattgtca gctgatgcct actttccaaa
    11041 attgttggaa ggagggcagc ttgcttcaca gccaacctta tcccgttttc tttccagaac
    11101 tgacgaggaa acagtccata gtttgcgatg cctcaacctt gaattggtcg aattcttttt
    11161 acagtttcac cagctaaacc aactcattgt agatatcgat tctacccatt tcacaactta
    11221 tggcaagcaa gaaggtgttg cttataacgc ccactatcgt gctcatggct atcatcctct
    11281 ttatgctttc gaggggaaga caggttattg tttcaatgcc cagcttcgtc ctggtaatcg
    11341 ttattgttct gaagaggcag acagctttat cacacctgtt ttagaacggt ttaatcaact
    11401 tctctttcga atggatagtg gctttgcgac cccaaaatta tacgatttaa ttgaaaaaac
    11461 agggcaatac tacctcataa aactcaagaa aaatactgtt ctgagccgtc ttggagacct
    11521 ttccctccct tgcccacagg atgaggactt aaccatcttg ccccactccg cctactcaga
    11581 aactctctat caagcaggat cttggtcgca caagcgtcgt gtctgccagt tctctgaacg
    11641 aaaagaagga aacttgttct acgatattat ttctctcgtt acaaatatga cgagtggaac
    11701 aagccaagac cagtttcagc tttatcgtgg acgtggtcaa gccgagaatt tcatcaagga
    11761 gatgaaggag ggattttttg gcgataaaac ggatagttca accttaatca aaaacgaagt
    11821 tcgtatgatg atgagctgta tcgcctacaa tctctatctt tttctcaaac atctagctgg
    11881 aggtgacttc caaactttaa caatcaaacg cttccaccat ctttttcttc acgtggtagg
    11941 aaactgtgtt cgaacaggac gcaagcagct cctcaaattg tctagtctct atgcctattc
    12001 cgaattgttt tcagcacttt attctaggat tagaaaagtc aacctgaatc ttcctgttcc
    12061 ttatgaacca cctggaagaa aagcgtcgtt aatgatgcat taaagaacag tcgagatgaa
    12121 aaaatcgtgt gacgcaccaa gggaggagtc tgcccttttg aggaaatcta gcgaggaaaa
    12181 acgatactgg aacagcagaa agtaaaactg acctcatgag gaggaagaaa gtggctcatg
    12241 aggtcagggg ttttgtaagt tacatctagt tgagagaggt atgaatgatt tgggtaaaag
    12301 atagtcttaa tctatatgtg taaatcacga aagtaattga attcggaata aggttggtgt
    12361 gaaaagacat agtaaactga atcttgaata ctagaatgat acttctacaa tattgttata
    12421 atttaatttg aattccaaaa tcacattgtt ccgtccgtat ttcattttac tgtatgtctt
    12481 cctaagctcc aaagtttctg cgtcaaattt cctattttca ctgtagtctt tgacacttcg
    12541 aacgacaatt tgaatcatat attcaaaaaa aatgaaaaat ttgtgccaac aagcctaaat
    12601 attctgaaaa tttgtttgat ttttaatgga taatgtgata taatggttgt caatctataa
    12661 ttcacataaa cagaggagta gaaaatgaaa aaaaatcgtg tatttgctac agcaggtctt
    12721 gttttattag cagcaggtgt acttgcagca tgcagttctt caaaatcatc tgattcatca
    12781 gcccctaaag cttatggcta tgtttataca gcagacccag aaaccttgga ctacctgatt
    12841 tcaagtaaaa atagtacaac agtagtgact tcaaatggga ttgatggttt attcactaac
    12901 gataattacg gtaatcttgc tcctgcagtt gcagaggatt gggaagtctc taaggatggt
    12961 ttgacctaca cttataagat tcgtaaaggg gttaaatggt ttacctctga tggagaagaa
    13021 tatgcagagg tgacggctaa agatttcgtg aacggtttaa aacacgcagc agataaaaaa
    13081 tcagaagcta tgtatttagc tgaaaattcg gttaaaggct tggcagatta tctatcagga
    13141 acttcaacag atttttcaac agttggtgtc aaggcggttg atgattatac gttacaatac
    13201 actttgaacc agcctgaacc gttctggaac tctaagttga cctattctat tttctggcct
    13261 ctgaatgaag aattcgaaac atcaaaagga agcgattttg ctaaaccaac agatccgaca
    13321 tccttgcttt ataatggtcc attcttgttg aaagggttga ctgcaaaatc ttctgtagag
    13381 tttgtaaaaa atgagcaata ttgggataaa gaaaatgtcc acctagatac tatcaatcta
    13441 gcttactatg atggatcaga tcaggagtcg ctagagcgta acttcactag tggagcctat
    13501 agttatgccc gtctttaccc taccagctcc aactattcta aggttgcaga agaatacaag
    13561 gacaatatct attacacaca atcaggctct gggattgctg gtctgggtgt gaatattgat
    13621 cgccaaagtt acaactatac ttctaaaact acagattcag agaaagtagc tactaagaag
    13681 gcattgctta acaaagattt ccgtcaagcc ttgaattttg ctcttgatcg ctcagcttac
    13741 tcagctcaaa tcaatggtaa agatggagca gctttagcag ttcgtaattt atttgtaaaa
    13801 ccagactttg tttcagctgg tgagaagacc tttggtgatt tagtcgctgc tcaacttcct
    13861 gcttatggtg atgagtggaa aggtgtgaat ttagctgatg ggcaggatgg tttattcaat
    13921 gctgacaagg ccaaggcaga gtttgcgaaa gctaagaaag ctttagaagc agacggcgtt
    13981 cagtttccta ttcatctgga cgttccagta gaccaagcat caaaaaacta tatatctcgt
    14041 attcagtcct ttaaacaatc tgtagaaaca gttcttggtg ttgaaaatgt cgttgttgat
    14101 attcaacaaa tgacaagtga tgaattcctt aatattactt actatgctgc caatgcttca
    14161 tctgaggatt gggatgtatc gggaggagtt tcatgggggc cagactatca agacccatct
    14221 acttacctgg atattttaaa aacaactagc agtgaaacta caaaaacata tttaggattt
    14281 gataatccaa atagcccttc agtagttcaa gttggtttga aagaatacga taaattagtt
    14341 gatgaagctg ccaaagagac aagcgacttg aatgtccgtt atgaaaaata tgcagcggct
    14401 caagcatggt tgacagatag ttcactcttt attcctgcta tggcttcttc tggtgcagca
    14461 ccagtgcttt cacgaattgt tccatttact ggagcttctg cgcaaacagg ctctaagggg
    14521 tcagatgttt acttcaaata tttgaaatta caagataaag cggtgactaa ggaagagtat
    14581 gaaaaagctc gtgaaaaatg gttgaaagaa aaagctgaat caaatgagaa agctcaaaaa
    14641 gaattggcaa gtcatgtgaa gtaaataatt tctaatagaa ctttctctcc atgaggagaa
    14701 ggttcttttg ggatttttaa aggaaataat atgaaaaaat atatttttat gcgtgttttg
    14761 cggtcattgg tttcgatttt cttagtaacg actttgacct acacgattat ctataccttg
    14821 gttcctcgaa aattgatttt caagcaggat cctaactata ataaaattgc gacaacggct
    14881 gataaacgtg ataactatga aaatactgtg tttgagcgta tgggctacat tgagtattac
    14941 gatactaaag agttgcaaga aaaggcaagt agcatggatt cttctgtaac agtagaagca
    15001 aatgcgacca ataaagctat ttatgaaaag tacatcaatc aattaggtca tggttggact
    15061 ttgggagaat ttactgaaag tggtcaattc tatgctactc gtgaaattcc aatttttgaa
    15121 cgtgtttttc acttctatgc taacttgatt gacattgacc atacaaataa aatccaagac
    15181 cctgaaaatc cagacttgaa acgctacctt cgttttgaaa atgatccagc tatcggatgg
    15241 tcattggtcg gttcaggaac taaacataaa tatctcttgt actttaacag tcagttccca
    15301 tttgttcatc aaaactttgt gaacttgaat ttaggtgact cttacccaac ctatgctaat
    15361 acaccagttc ttcaggttat tactcaaggt caaggacaaa ccaaaactgc ccaagttcag
    15421 ttcccaacag gtaagaaaac gtcttctgta aatatttact caagaaccta caagtcacct
    15481 agtcaggctg actctcgtga agtagctagc tatgggaaag atgatcctta tacagcgact
    15541 gaaagtaatt accaatatcc atctatgatt gtcagctctg ctattactgg tttgattggt
    15601 ttggttcttg cctatgctct tgccgtgcca cttggttcag ccatggctcg tttcaagaac
    15661 acttggattg atagcctctc aacaggggct ttgaccttct tgcttgctct tccaacgatt
    15721 gccttggttt acatcgttcg attgattgga tcatctattt cccttccaga ttcattccct
    15781 atcttgggag ctggagattg gcgttcttac gttttaccag cagtcatcct tggtttgttg
    15841 ggtgctcctg gtacagccat ttggattcgt cgttacatga ttgacttgca atctcaagac
    15901 tttgttcgtt tcgctcgtgc aaaaggtttg tctgaaaaag aaatttcaaa caaacacatc
    15961 tttaaaaatg ccatggttcc gctggtttca ggaattcctg gtgccattat tggggttatc
    16021 ggtggtgcaa cccttactga aacagtcttc gccttcccag gtatgggtaa aatgttgatt
    16081 gactctgtaa aagcatctaa taactctatg gtcgttggtc ttgtcttcat ctttacatgt
    16141 atttctatct tctcacgtct tttgggagat atttggatga ctattattga cccacgtatt
    16201 aaattgactg agaaaggagg caaataatgt ctacaatcga taaagaaaaa tttcagtttg
    16261 taaaacgtga cgattttgcc tctgaaacta ttgatgcgcc agcatattct tactggaaat
    16321 cagtgtttaa acaatttatg aagaaaaaat caactgtagt catgttggga atcttggtag
    16381 ccatcatttt gataagtttc atctacccaa tgttttctaa gtttgatttc aatgatgtca
    16441 gcaaggtaaa cgactttagt gttcgttata tcaagccaaa tgcggagcat tggttcggta
    16501 ctgacagtaa cggtaaatcg ctctttgacg gtgtctggtt cggagctcgt aactccatcc
    16561 tcatttctgt gattgcgaca gtgattaact tggttatcgg tgtttttgtc ggtggtattt
    16621 ggggtatttc aaaatcagtt gaccgtgtca tgatggaagt ttacaacgtc atctcaaaca
    16681 tcccacctct tttgattgtt attgtcttaa cttactcaat cggagctgga ttctggaatc
    16741 tgatttttgc catgagcgta acaacatgga ttggtattgc cttcatgatc cgtgtgcaaa
    16801 tcttgcgcta tcgtgacttg gaatacaact tggcgtcacg tactttggga acaccaacct
    16861 tgaagattgt tgccaaaaat atcatgcctc aattggtatc tgttattgtg acaaccatga
    16921 ctcaaatgct tccaagcttt atctcatacg aagccttctt gtctttcttc ggtcttggat
    16981 taccgattac agtgccaagt ttgggtcgtt tgatttcgga ttattcacaa aacgtaacaa
    17041 ccaatgctta cttgttctgg attccattga caacccttgt cttggtatcc ttgtcccttt
    17101 tcgtagttgg tcaaaactta gcggatgcta gtgatccacg tacacataga taggagtaga
    17161 aatgacaaaa gaaaaaaatg taattttgac tgctcgcgat attgtcgtgg aatttgacgt
    17221 tcgtgacaaa gtattgacag ccattcgcgg cgtttccctt gaactagtcg aaggagaagt
    17281 attagccttg gtaggtgagt caggatcagg taaatctgtt ttgacaaaga ccttcacagg
    17341 tatgctcgaa gaaaatggtc gtattgccca aggtagtatt gactaccgtg gtcaggactt
    17401 gacagcttta tcttctcaca aggattggga acaaattcgt ggtgctaaga ttgcgactat
    17461 cttccaggac ccaatgacta gtttggaccc cattaaaaca attggtagtc agattacaga
    17521 agttattgta aaacaccaag gaaaaacagc taaagaagcg aaagaattgg ccattgacta
    17581 catgaataag gttggcattc cagacgcaga tagacgtttt aatgaatacc cattccaata
    17641 ttctggagga atgcgtcaac gtatcgttat tgcgattgcc cttgcctgcc gacctgatgt
    17701 cttgatctgt gatgagccaa caactgcctt ggatgtgact attcaagctc agattattga
    17761 tttgctaaaa tctttacaaa acgagtatca tttcacaaca atctttatta cccacgacct
    17821 tggtgtggtg gcaagtattg cggataaggt agcggttatg tatgcaggag aaatcgttga
    17881 gtatggaacg gttgaggaag tcttctatga ccctcgccat ccatatacat ggagtctctt
    17941 gtctagcttg cctcagcttg ctgatgataa aggggaactt tactcaatcc caggaacacc
    18001 tccgtcactt tatactgacc tgaaagggga tgcttttgcc ttgcgttctg actacgcaat
    18061 gcagattgac ttcgaacaaa aagctcctca attctcagta tcagagacac attgggctaa
    18121 aacttggctt cttcatgagg atgctccaaa agtagaaaaa ccagctgtga ttgcaaatct
    18181 ccatgataag atccgtgaaa aaatgggatt tgcccatctg gctgactagg aggaaggaaa
    18241 tgtctgaaaa attagtagaa atcaaagatt tagaaatttc cttcggtgaa ggaagtaaga
    18301 agtttgtcgc ggttaaaaat gctaacttct ttatcaacaa gggagaaact ttctcgcttg
    18361 taggtgagtc cggtagtggg aaaacaacta ttggtcgtgc tatcatcggt ctaaatgata
    18421 caagtaatgg agatatcatt tttgatggtc aaaagattaa tggtaagaaa tcgcgtgaac
    18481 aagctgcgga attgattcgt cgaatccaga tgattttcca agaccctgcc gcaagtttga
    18541 atgaacgtgc gactgttgat tatattattt ctgaaggtct ttacaatcac cgtttattta
    18601 aggatgaaga agaacgtaaa gagaaagttc aaaatattat ccgtgaagta ggtcttcttg
    18661 ctgagcactt gactcgttac cctcatgaat tctcaggcgg tcaacgtcaa cgtatcggta
    18721 ttgcccgtgc cttggtcatg caaccagact ttgttattgc agatgagcca atttcagcct
    18781 tggacgtttc tgtacgtgcc caagtcttga acttgctcaa aaaattccaa aaagagctcg
    18841 gcctgaccta tctcttcatc gcccatgact tgtcggttgt tcgctttatt tcagatcgta
    18901 tcgcagttat ttacaagggt gttattgtag aggttgcaga aacagaagaa ttgtttaaca
    18961 atccaattca cccatatact caagccttgc tttcagcggt accaatccca gatccaatct
    19021 tggaacgtaa gaaggtcttg aaggtttacg acccaagtca acacgactat gagactgata
    19081 agccatctat ggtagaaatc cgtccaggtc actatgtttg ggcgaaccaa gccgaattgg
    19141 cacgttatca aaaaggacta aactagtaat ggttttataa tttccatgtc aatttttatg
    19201 gaaattataa aacttttttt gttggactga tttgaaaaac tttgaaagct tcctgaaaga
    19261 atttttgaaa aattttaaaa attatgaaat gttattgaca ggtgatgaaa aaggtggtag
    19321 gacaggaagc atcaaaaaag aaagcagaaa aagaaatgaa taagagacaa gccgtaacta
    19381 tgaatcaaaa cttttacttt agctacttta gatagtgttt gtagacatgt caccatggat
    19441 gcaaacagtt caagcaattt gtttgtatct atgtggctaa gatttttgat tgtggtccat
    19501 atagatgaat atctaaatgg actagctaag ttgtaacttg ttaggttatt tcaagtatcc
    19561 gtttagatag tatctaggcg gttttttgtt ttatcttttc tgagaaggag tttcattatg
    19621 aaagttgttc gaaaatcact agccccctct tggtagtggg gatcctcttg acttctctga
    19681 tcagtttgca tcagttgaag gcagataaga aaagagatgt ctttcgtatc ggtatttcgc
    19741 agtttattac ccaccagtcc ttagacgcta ctagagaagg atttgtggat gaactggcca
    19801 aacaaggcta tgttgagggg gaaaatattg agattgattt gcaaaatgca cagggagaac
    19861 aaagaaatct aaaaacgatt tcccagcaac tagcagaatc cagcgatgtc gttctagcca
    19921 tcgcaaggcc ttctgctcag agcgagccca attcggttgt tcaaaaggat gaagctaagc
    19981 gacttctgaa agaaaaaggc tttaccgttg ttgaaaaaac aatcttggac agtaataacg
    20041 tcaaggcggc agcagagagt ttgatggcag aggtggatat ggtttttgtc ccaactgata
    20101 atatcatttc gttaaccatg gaaacagtta agcaggtttc tattaaacac aaggttccag
    20161 tattcggtgg ttcaacagaa atgattgctg ttggtggctt gtataactac ggaaccaatt
    20221 atgaagaatt gggaagacag acagctcgca tgctgattca tgttttaaaa ggtgagaaac
    20281 cagaaaatat agcagttgag ttgcctgaaa aactggaatt gcatactaat caagaaatgg
    20341 cagatgcatt gggaattgat attagtaagt tagaaggcaa ggaataagga ggattaagat
    20401 gaattttgtg ttatctagtt tatcagaagg tttgttgtgg tcgattgtgg cgattggggt
    20461 ctacttgact ttccgtattt tggatattgc ggatatgact gctgagggag cctttcctct
    20521 gggggcagca gtcgttgtat cacagataca ggcaggggca aatccttggc ttgcgacttt
    20581 acttgctttg ctggcaggta tggtagcagg tcttgtatca ggaatgctcc acaccaagat
    20641 gaaaatccca gctctcttga cagggattgt gaccttgaca gggctctatt caatcaatat
    20701 taaaatcatg ggcagtgtac ccaatctttc cttgggagat tctgcaaccg tctttaaaca
    20761 gttggcgagc ttgggtctga caaatgaagg aactgttttc tcacttagtt tggcctgttt
    20821 tttacttgtt tgtttggtct tgaccctttt gatgaaaaca gagattggct tggtcttgcg
    20881 ttcgactggg gataatatcc cgatgagtga ggccaatgga gtcaatgtag acaccatgaa
    20941 gattgttggt tacatgattt caaacggttt gattgctcta tgtggttcct tgtttgccca
    21001 aaatgatgga ttttcagatg tgacttctgg gacaggaacc attgttgttg gtttgagctc
    21061 ggtgattatt gtggaagtct tgatacacga cttgaccatt ggaggccgct tattatccat
    21121 cggaatcggt gctattgttt accgtttgat tattttaaat atctatgaaa ttccaaatct
    21181 agatcaaaac ttggtacgtc tctttaatgc aatcttgctc gctttggttt tatttgcacc
    21241 agaattgcaa aagagattaa agattcgtgg tctgaaattg agaaatgaat aggaggagaa
    21301 agttatggca agtttgttaa cacttgaaaa tattcacaag acatttgaag cagggacggt
    21361 caatgagaac catgtcctca aaggattaga tttagaggtt gaagagggag attttatctc
    21421 tgtgattggt ggaaatggag caggaaaatc cactttgatg aatatcttgg ctggcaatct
    21481 atcggtggac gaaggggacc ttctactggc agggaaatcc attaaaaatc tgagtgtcag
    21541 aaagagagcc aaggatattg cccgtgtttt tcaagatcct aagatgggaa cagcttctcg
    21601 tttgacgatt gaggagaata tggctattgc cttgagacgc gggcagaaga gagggcttgg
    21661 ttggggcgtg aaggagaagg atagaatcca gttccaagag gccttgaagg agttgaatat
    21721 tggtcttgaa aaccgcttaa aagtagatac tcaatatctc tcaggaggac aaagacaggc
    21781 tttgactcta gtcatgacag atctgatgaa accaaaactt ttacttttgg atgaacatac
    21841 tgcggcactt gacccgaaaa ccagtcaaat ggtgatggac ttgacgcaaa agattgtgga
    21901 gcaccatcag tggacgactt tgatgattac ccatgatatg aatcatgcga ttgagtacgg
    21961 caatcgtctc attatgctct atcaaggcaa gatagtggtg gatgtcaagg gagaagagaa
    22021 aaagcatctg acagttgaag accttatgca tctcttccag aaaaatagtg gccaaagtct
    22081 ggtcagtgat gaattagttt tgggataaag aaaaaggctg agatctggtg tctcagtctt
    22141 ttatttcttg cgttttgcaa aatctacaaa tctaaatttt tctaacttat ggcgactttc
    22201 ggtaaactga aattggcgtc cgtcttggag atagactttt gatttaatcg aaacgacata
    22261 agggtctttg ccaatgtcca tgagaatctt gtctcggtcg cttgagtggt caatggtgat
    22321 ttccttctga gcataatcaa taaggagttt gaggccattt tctatataag aatagataga
    22381 ctgctcagca atttggcgag tgagatttgg gattagttcc atatccagat agtccgtatc
    22441 cagaacggat accagatcat ccaccacacg ctggcggacc accttccaaa ccatccgaaa
    22501 ctctgggaaa ccggttatca gtgaggattt tttatcaata ataatcttgt ccagactgac
    22561 cacgttggtt ttagagcgca gtccaagttc tttaactagt tcttggtagc tggttaggtt
    22621 ggatacaggg aaattgacgg tttcttcttt gacgacttga gaaccttgcc ctcttatctt
    22681 tttgatcaat ccttcctctt ggaggagaga cagggccttt cggacggtat cacgactcac
    22741 ttgatattgc tccataaggt cgtgctcgct aggaagaaaa tctccgacag cgtaagtctc
    22801 gttttgaatg gtttcttgga tttgcttaaa taattgttgg tattttttca ttttgctttc
    22861 ttaaataggt ttttagtcta gtttaatgaa cacctgtatc tattttacca tacaagtgct
    22921 agaaaattta caaaaaaaca ccctttttat accaaacaag ttgcagacaa gtttggtatc
    22981 gtttacaata tacttatcaa atcaaacttg ctttgacaag tataaggaga atcaaatggg
    23041 aaaatttgaa caagaagcca aagatttgct tcaggcaatt ggaggtaaag agaatgtgac
    23101 tgccgtcacc cactgtgcga cacggatgcg gtttgtttta ggagatgata agaaggctaa
    23161 tgttaaagct atcgagtcaa ttccagctgt taaaggaacc tttacaaatg caggtcaatt
    23221 tcaggtaatc attggaaatg acgtgcccat cttttataat gattttacag ccgtttcagg
    23281 tattgagggt gtttccaaag aagcagccaa gtctgcagct aagagtaatc aaaacgtggt
    23341 ccaaggtgtt atgaccactc tggcggaaat ctttactccg attattccag ccttgattgt
    23401 tggtggattg atcctcggtt tccgtaatgt cttggaaggt gtccattggt cgatgttgga
    23461 tggcaagacc atcacagaat cctctcagtt ttgggcaggg gttaatcact tcctctggtt
    23521 gcctggtgaa gctatcttcc agttcttacc agtagggatt acttggtctg tttctcgtaa
    23581 gatgggaacc agccaaattt tgggaattgt tctcggaatc tgtttggtat cgcctcagtt
    23641 gctcaatgcc tatgcggttg cttcaacgcc agcagctgat atcgcagcaa actgggtttg
    23701 gaattttggt tattttactg ttaatcgtat tggttaccaa gcccaagtta tcccagcctt
    23761 gcttgcaggt ttgagtctgt cttatcttga aattttttgg cgcaaacata tcccagaagt
    23821 catttccatg atttttgtac cttttttgtc attgattcca gccttgattt tggctcatac
    23881 tgtcttgggg ccaatcggtt ggacaattgg acaaggactt tcatcagttg tattggcagg
    23941 tttaacgggt cctgtaaaat ggctcttcgg tgcaattttt ggtgccctct acgctccatt
    24001 tgtcatcaca ggtctgcacc acatgaccaa tgccattgat acacaattga ttgcggatgc
    24061 tggtggcact gccctctggc ctatgattgc tctttctaat attgctcaag gctcagccgt
    24121 gtttgcctat tatttcatgc atcgccatga tgagcgtgag gctcaggttt cacttcctgc
    24181 aaccatttca gcctatctcg gtgttacaga accagctctt tttggggtta acgtaaaata
    24241 tatttatcca tttgttgctg ggatgactgg ttcagccctt gcaggcatgt tatccgttac
    24301 ttttaatgta actgcggctt ctattggtat cggtggtttg ccaggtattc tctctattca
    24361 acctcaatac atgctgccat ttgcaggaac tatgctagtt gcgattgttg ttccaatgct
    24421 cttgactttc ttcttccgca aggctggtct ctttacaaaa acagagggcg atacgaactt
    24481 gcaggcagaa ttcgttgctc aagaagaagc agaatttgtg agccatgaac tagtagaact
    24541 tacttcggta gaaattatca gcccactaac tggccaagtg aaagaattga gtcaagcgac
    24601 ggatcctgtt tttgcatcag gtgtcatggg gcaaggtctg gtcattgaac caagccaagg
    24661 tgagttgacc tctccagtta atggaacagt gacggttctt ttccctacca agcatgccat
    24721 cggcattgtc tctgacgagg gagttgaatt gctcatccac atcggtatgg atacagtagg
    24781 tcttgatggc aaaggttttg aaagtcttgt agtccaagga gatcacgtta tagttggcca
    24841 gcaactgatt cgttttgata tggatgtcat taaggctgca ggtctggtga cagaaactct
    24901 tgttatcatc accaaccaag atgcttatac agcgactatt cccggaactt atccgacaac
    24961 gatccaagct ggagcatctc tcatggtcgc tacacgaatc taatactctt cgaaaatctc
    25021 ttcaaaccac gtcagctcta tctgcaacct caaaatagtg ttttgagcaa cctgtggcta
    25081 gcttcctagt ttgttctttg attttcattg agtataattg aaatagcaca aatagggacg
    25141 gaagactgct tcctcccttt tatcagaaaa ggagaaacag atgacacttg ataaaggaaa
    25201 agtagtctat caaatctatc caaaatctta caaagacacc actgaaaacg gtattgggga
    25261 tttccgtggg attatcggaa aaattcccta tctagccaaa ctaggtgtgg atatggtctg
    25321 gctcaatcca ttctatccaa gtcctcaacg ggataatggt tacgatattt cagattatat
    25381 ggcagtggat cctctttttg gtgatatggc tgattttgag gaaatggtgt gtatcggtaa
    25441 agagcacaag attgacttta tgctggacat ggtacttaat cattgttcga cagagcatga
    25501 atggtttcag aaagccctag ctggtgataa gtattatcaa gactttttct tcatccaaga
    25561 ccaaccaaca gattggcagt ctaagtttgg tggttctgca tgggcgcctt tcggggatac
    25621 tgggaaatac tatcttcacc tatttgatga gacccaggct gaccttaact ggcgcaatcc
    25681 caatgtccgt aaggagcttt tcaaggttgt taatttctgg cgcgacaagg gtgtcaaagg
    25741 ttttcgattt gatgtgatca atttgatcgg taaggacgag gtttcagtgg actgtcctga
    25801 aaatgaaggg aagccagctt atacagacaa acccatcgtt cataactatt tgcgtatgat
    25861 gaaccaagcc acttttggat ctgacgatag ctttatgaca gttggggaaa tgtcttctac
    25921 caccatggaa aactgtgtcc tttattcgtc gcctgatcgt caggaattat ccatgacctt
    25981 taattttcat cacctcaagg tggactataa ggatggacaa aagtggacct tggctccctt
    26041 tgattttgaa gagttgaaga gtctttacca tagttggggt aaggaaatga gtgataaaga
    26101 cggttggagt gccctctttt ggaacaatca cgatcaacca cgtgccttga accgttttgt
    26161 cgatattcaa aacttccgca aggaaggagc caccatgcta gcggccagca ttcacctgtc
    26221 acgtgggaca ccttatatct acatgggtga ggaaatcggg atgattgacc cagactatga
    26281 ttctatggct gattatgtgg atgtcgaatc gcttaatgcc tatcagatgc tcttagaaga
    26341 aggaaagagt cagcaagaag ccttccagat tattcaagct aagtcgcgtg ataattcacg
    26401 cattcccatg cagtgggatg cttcggaaaa tgcaggattt tcaacaggca ctccttggtt
    26461 gaaagctggt aaatcctata aagacatcaa tgtagaaaat gaaatccagg gtccgatttt
    26521 taccttctat caagacttga ttcgactgcg taaggaaatg ccaatcattt cagaaggaag
    26581 ttacaaacca gcctttgaag atagcaaaca agtctacgct tttgaacgcc agtttgagga
    26641 tcaaaagtta ctagtgctca ataactttta tgctaaagaa gtggaaatcg acttgccagc
    26701 agtctaccaa aatggacaga ttttgatatc aaactatgaa gatgcagaag tgtctgagaa
    26761 aattctactc aaaccctatc aaacactggc tatttatgta aattaatact cttcgcaaat
    26821 ctcttcaaac cgtgtcagtt ttatctgcaa cctcaaagcg gtgctttgag caacctgtgg
    26881 ctagcttcct agtttgctct ttgattttca ttgagtataa acttagcaag gagacctctt
    26941 tttcagaaaa gcagggttct ttttgctgtc tattccatcc ctgaaagtgt agaacaaact
    27001 ggtcagggtg gctagtttgt ggtataatga aagggactca aaaagaaaca ggagaaaaat
    27061 gatggattta cttttagcaa ttgtattgat tgtgctagct tttctaggag gagctcttgg
    27121 aggaatgtac ttggttcgta agcaaatcga aaaagaattt gctgacaacc cacgtttgaa
    27181 tgctgaagca gttcgtactc ttttgagtgc aaatggtcaa aaaccaagcg aagctaaggt
    27241 acaacaagtt taccaccaaa tcatccgcca acaaaaggca gcccttgcta acaataaaaa
    27301 gaaaaaataa ataaggaaaa gtctgggatg aaagttccag acttctcact atgttggtta
    27361 tggtttaggg atgagacttt ttccttgcat tctattgata tttgattatg attaagagag
    27421 aggtagttga cattctatca agctgtcggt cagttcgttc aggacaagga atcataatga
    27481 acgatcaatc agaaaaaaga ctagaaagaa gactgtatgg ataatcgacc aattggtttt
    27541 ttggattcgg gtgtcggggg cttgaccgtt gtgcgcgagc tcatgcgcca gcttccccat
    27601 gaagaaatcg tctatattgg agattcggcg cgggcgccct atggcccccg tcctgctgag
    27661 caaattcgtg aatatacttg gcagctggtc aactttctct tgaccaagga tgtcaaaatg
    27721 attgtcattg cttgtaacac tgcgactgcg gtcgtctggg aagaaatcaa ggctcaacta
    27781 gatattcctg tcttgggtgt aattttgcca ggagcttcgg cagccatcaa gtccagtcaa
    27841 ggtgggaaaa tcggagtgat tggaacgccc atgacggtac aatcagacat ataccgtcag
    27901 aaaatccatg atctggatcc cgacttacag gtggagagct tggcctgtcc caagtttgct
    27961 cccttggttg agtcaggtgc cctgtcaacc agtgttacca agaaggtggt ctatgaaacc
    28021 ctgcgtccct tggttggaaa ggtggatagc ctgattttgg gctgtactca ttatccactc
    28081 cttcgcccta ttatccaaaa tgtgatgggg ccaaaggttc agctcatcga tagtggggca
    28141 gagtgcgtac gggatatctc agtcttactc aattattttg aaatcaatcg tggtcgcgat
    28201 gctggaccac tccatcaccg tttttacaca acagccagta gccaaagttt tgcacaaatt
    28261 ggtgaagaat ggctggaaaa agagattcat gtggagcatg tagaattatg acaaataaaa
    28321 tttatgaata taaggatgac caggactggt atgttgggtc ttatagtatt tttggtggcg
    28381 ttaacagttt gagcgactat aagacagatt ttcctctgtt tgaattctcc aaaatatttg
    28441 gagatgaaga gtatggtttc ccgctttcag ttactgtttt acgctatggt tctacctacc
    28501 gtttgttctc ctttgtggta gatatgctta atcaagaaat gggacgaaac ttggaagtta
    28561 ttcaacgtca tggggccctg ctcttggttg aaaatgggca actcttgtat gtagaattgc
    28621 ctaaagaagg ggtcaatgtt catgatttct ttgagacaag caaggtcaga gaaaccttgt
    28681 tgattgcgac tcgtaacgaa ggcaaaacca aggaattccg agctatcttt gataagttag
    28741 gctacgatgt ggaaaatctt aatgactacc ctgacctgcc tgaagtagca gaaacaggta
    28801 tgacctttga agaaaatgcc cgccttaagg cagaaaccat ttctcaatta acgggtaaga
    28861 tggttttggc agatgattct ggtctcaaag tcgatgtcct tggtggctta ccaggcgtct
    28921 ggtcagctcg tttcgcaggt gtgggagcaa ctgaccgtga aaataatgcc aaactcttgc
    28981 acgaattggc catggtcttt gaactcaagg accgctcggc tcagttccac acaaccctag
    29041 tcgtagccag cccaaataag gaaagtttag ttgttgaagc agactggtca ggttatatta
    29101 actttgaacc taagggtgaa aatggctttg gctatgatcc cctcttcctt gtaggagaga
    29161 caggtgagtc atcagctgaa ttaaccctag aagaaaaaaa tagtcaatct caccgtgcct
    29221 tagccgttaa gaaacttttg gaggtatttc catcatggca aagcaaacca tcattgtaat
    29281 gagcgattcc catggcgata gcttgattgt ggaagaagtc cgtgatcgct atgtgggcaa
    29341 agtcgatgcc gtttttcata acggcgattc tgaactacgt ccggattctc cactttggga
    29401 gggcatccgc gttgttaaag ggaacatgga cttctacgcc ggctacccag aacgtctggt
    29461 gactgagctt ggttcgacca agattatcca aactcatggt cacttgtttg acatcaattt
    29521 caactttcaa aagttggact actgggctca ggaggaagag gccgctatct gcctctatgg
    29581 tcacttgcat gtgccaagtg cttggttgga aggcaagatc ctctttctaa atccaggttc
    29641 tatcagtcaa ccacgaggta ccatcagaga atgtctctat gctcgtgtgg agattgatga
    29701 tagttacttc aaagtggact ttttgacacg agatcacgag gtgtatccag gtttgtccaa
    29761 ggagtttagc cgatgattgc caaggagttt gagactttct tgttggggca ggaggaaact
    29821 tttttgaccc ctgctaaaaa tctagctgtg ttgattgata cccacaatgc ggatcatgcg
    29881 accctcttgc tcagtcagat gacctatacc cgtgttcccg ttgtgacaga tgaaaaacag
    29941 tttgttggga cgattggact cagagatatt atggcttatc agatggagca tgacttgagc
    30001 caagaaatca tggcggatac ggatatcgtt catatgacaa aaacggacgt agcggttgtt
    30061 tcgcctgatt tcaccattac ggaggtcttg cacaagctag tagatgagtc cttcttaccg
    30121 gtcgtggatg cagagggtat tttccaaggg attattacgc gcaagtccat cctcaaggcc
    30181 gttaatgccc tcttgcatga ctttagtaag gaatatgaga ttcgatgcca atgagagaca
    30241 ggatttcagc ctttttagag gaaaagcagg gcttgtctgt caattccaag cagtcctata
    30301 agtatgattt ggagcaattt ttagacatgg taggtgagcg gatttctgag accagtctca
    30361 agatttacca agcccagcta gccaatctaa aaatcagcgc ccagaagcga aagatttcgg
    30421 cctgtaacca atttctatac tttctctatc aaaaaggaga ggtggacagc ttttaccgct
    30481 tggaattagc caaacaagct gaaaagaaga cggaaaagcc agagattcta tacctagact
    30541 ctttttggca ggaaagcgac catccagagg gccgcttgct agcgctctta atcctagaaa
    30601 tggggctctt gcccagtgag attttagcca tcaaggttgc ggacatcaat ctggattttc
    30661 aggtgctgcg aatcagcaag gcttcccaac agaggattgt caccattccc acggccttgc
    30721 tttcagaatt ggaacccttg atggggcaga cctatctttt tgaaagagga gagaaaccct
    30781 attctcgtca gtgggccttt cgtcagttag aatcttttgt caaggagaaa ggttttccat
    30841 ccttatcagc tcaagtctta cgtgaacagt ttattctaag acaaatagaa aacaaggtcg
    30901 atttgtacga aattgcaaaa aaattaggat taaaaacagt cctgacctta gaaaaatata
    30961 gataatggat attaaattaa aagattttga aggacccctg gacttgctct tgcatctggt
    31021 ttctaagtac cagatggata tctacgatgt gcccattacg gaagtcatcg aacagtatct
    31081 agcctatgtc tcaaccctgc aggccatgcg tctggaagtg acgggtgagt acatggtcat
    31141 ggctagtcag ctcatgctga ttaagagtcg taaactcctt ccgaaggtag cagaagtgac
    31201 agacttgggg gatgacctgg agcaggacct cctctctcaa atcgaagaat atcgcaagtt
    31261 caagctcttg ggtgagcact tggaagccaa gcaccaagaa cgggcccagt attattccaa
    31321 agcgccgaca gagttgattt acgaagatgc ggagcttgtg catgacaaga cgaccattga
    31381 cctctttttg gctttttcaa atatcctagc caagaaaaaa gaggagtttg cacaaaatca
    31441 cacgacgatc ttgcgggatg agtataagat tgaggacatg atgattatcg tgaaagagtc
    31501 cttgattgga cgagatcaat tgcgcttgca ggatttgttc aaggaagccc agaatgtcca
    31561 agaggtcatc accctctttt tggcaaccct agagttaatc aaaacccagg agctgatcct
    31621 cgtgcaagag gagagttttg gagatatcta tctcatggaa aagaaggaag aaagtcaagt
    31681 gcctcaaagc tagacttgat agagaggaaa gatgagtact ttagcaaaaa tagaagcgct
    31741 cttgtttgta gcgggtgaag atgggattcg ggtccgccag ttagctgaac tcctctctct
    31801 gccaccgaca ggcatccagc aaagtttagg aaaattagcc cagaagtatg aaaaggaccc
    31861 agattccagt ttggctttga ttgagacaag tggtgcttat agattggtga ccaagcctca
    31921 atttgcagag attttgaagg aatactctaa ggcgcctatc aaccagagct tgtctcgggc
    31981 tgcccttgag accttgtcca ttattgccta caaacagccg attacgcgga tagaaattga
    32041 tgccatccgt ggagttaact cgagtggagc cttggcaaag ttgcaggctt ttgacctgat
    32101 aaaggaagac gggaaaaagg aagtattggg gcgccccaac ctctatgtga ctacggatta
    32161 tttcctagat tacatgggga taaaccattt agaagaatta ccagtgattg atgagcttga
    32221 gattcaagcc caagaaagcc aattatttgg tgaaaggata gaagaagatg agaatcaata
    32281 agtatattgc ccacgcaggt gtggccagta ggagaaaagc agaagagctg attaagcaag
    32341 gcttggtgac ggttaacggc caagtggtgc gtgaactagc aaccactatc aagtcaggcg
    32401 acaaggtcga agttgaaggt caacctatct acaacgaaga aaaggtctac tatctgctta
    32461 acaaaccacg cggtgtgatt tccagtgtga cagatgataa gggtcgcaag acggttgtcg
    32521 acctcttgcc caatgtcaaa gagcgtattt accctgtggg tcgtttggac tgggatacat
    32581 caggtgtctt gattttgacc aatgatgggg actttacaga cgagatgatt caccctcgta
    32641 atgagattga caaggtttat gtcgcgcgtg ttaaaggtgt ggccaataag gacaatctcc
    32701 gccccttgac ccgtggtctt gagattgatg gtaagaaaac caagccagct gtttatgaaa
    32761 ttctcaaagt ggacccagtc aaaaatcgct ctgtggtgca gttgaccatc catgaagggc
    32821 gtaaccatca ggttaaaaag atgtttgaag ctgttggtct ccaagtagat aagttgtctc
    32881 ggactcgttt cggacaccta gacttgacag gactccgtcc aggagaatcc cgtcgtctta
    32941 ataaaaaaga aatcagccaa ctacacacca tggctgtaac taagaaataa tgaaacgaat
    33001 tttaatagcg cctgtgcgct tttaccaacg ttttatctca ccagtctttc caccctcttg
    33061 tcgctttgat ctgacttgtt ccaactacat gattcaggct attgaaaaac atggttttaa
    33121 gggagtattg atgggcttgg ctcggatttt acgttgtcat ccctggtcga aaacaggtaa
    33181 ggaccccgtt ccagaccact tttcccttaa acgaaatcaa gaaggggaat gaggtggggt
    33241 aaatagattt caaaatgata aaaacgcatc ctatcaggtt tgagtgaact tgataggatg
    33301 cgttttagaa tgtcaaaatt ttatactctt cgaaaatccc ttcaaaccgc gtcagctttc
    33361 atctgcaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgctcttt
    33421 gattttcatt gagtattaaa ttgagtttga agtggcttat ttcaaagctt tttgtatgtc
    33481 ttcaatcatg agttttgttg attcaagtcc gcctccgctt agataccaga ggtctggtgt
    33541 tagttggata atcttaccat ttttagcagc aggtgtttca gcgataaggg cattttctag
    33601 gacaccgtcg ttgctagagt tgtccccacc gatggcaagg gtacggttga tgacaaagag
    33661 gatgtcaggg ttgatttctt tgacactttc aaagctgact tcttgtccgt ggcgtgagtc
    33721 ttcaaatttt gtatcagttg gtttgaattt caaggtttgg tacaagaaag agaaacgaga
    33781 tttggcacca aaggctgcca tttttccttc attaaggagg atcgcaaggg cttttttgtc
    33841 agagctttca tttttagtag cgacttcttg gatgctcttg tctagcttgg ccaattcttc
    33901 cttggctttt tgtgtactag tttcgccgaa ggcacttgct aaggattcga tattagcctt
    33961 ggtagaagtc cagtagtcat ccttgcttgc ttggaagaga acggttgggg cgatttcttt
    34021 gaatttgtct acgaattttt gtgtacgtgg tgaagcgata atcaaatcag gctcaagggc
    34081 ggcgatagct tctaaatcag gttctttcat agaaccaaca tttttgacag ttcccactag
    34141 gtcttttaga taagtcggaa cagtttttgt aggcattccg acgatatttt tttcaaatcc
    34201 taaagcgcga atagtatccg cagcgccgag gtcaaaggtc acaatctttt caggaacttt
    34261 ggaaagtttg acctcgtcca gtgaactttt aatggttatc tctgttggag cagagctact
    34321 ggtctctgtc tgactagtgc ttgagtttgt actacatgca ccaagtagga gcaagaagct
    34381 ggccactagg gcagtgaaat aaagtttaag ggatgttttc ataatttctc ctttttaaaa
    34441 tgtgataacg atttagggag tctcttaatc ttattgacta agagactgaa ggttctctaa
    34501 cttgagcttt tatgttacta gctatagata cagatctttt tgtcattgat atcagctagc
    34561 gtgatgggaa tctcataaag ttgactgagt aggtcagcct gcatgatttg atcggttctt
    34621 cccttgctaa agacctggcc gtccttgaag gcgacaattt catctgcata ctgactggcc
    34681 atgttgatat cgtggaggac gatgataatg gtcttgccga gttcctccac cagtcgtcga
    34741 agaatctgca tcatgctgac gctttgcttg atatcgagat tgttgagtgg ttcgtccagc
    34801 aagataaagt ccgtatcctg ggccagtacc atagcgataa agacgcgctg gagttgcccc
    34861 cctgacaggc tattgatgta gcggtctttt aagttggtca gttccaaata gttcagagtt
    34921 tctcggattt tttcccagtc ttctgatcta agtcgacctc ggctgtaggg aaaacgtcca
    34981 aaactgacca gttcttcaac agtcaatttg gcttggtaat tgattttctg ttttaggatg
    35041 gttagttctt gggccagttc ttgcgaattc cagctctcga tttcacgtcc tttgatactg
    35101 agaactccct gatctttctt ggttagtctg ctcatgatgg agaggagagt cgattttcca
    35161 gcaccatttg gaccaataaa ggctgtcagt ttttgaggac tgacttcaag cgaaatgcct
    35221 tgcaaaatat cctgtttttg aatggatttg tcaatgtttt ccagtttcac tgacgagacc
    35281 tcctatatag taagataaag aataagaagc cacccacact ctcaatgatc atactgatac
    35341 gaatttccag tgcaaagact cgttcaatca aggcttgccc caaggttaag ctaataaatc
    35401 caatcagaat ggccactata aagagtaact tgtgctgata gtctttgaca atcaggtagg
    35461 tgaggttggc cagcataaag ccgaagaagg ccataggtcc taccaaggca gtggccgttg
    35521 aggtcaaaag cacgattccc cagaggagct ctttctgttc tttttcaaca tcgagtccca
    35581 atatctgagc cgtttctctt tgcaggtgca agacatctag aacgactgct tttcgaaaga
    35641 aaaagattgt caaagcgagg atgatcagag aaccgatggc taggatggaa gtgttgagat
    35701 gttgaaagga ggcaaaaaga ctattttgca gtttatcgta ttcgtttgga tccattagga
    35761 cttgaaggaa ggtgctgata tttcgaaaga gacttctgag cgctagacag atcagcagga
    35821 tgaagaccag gtcttgcttc atcagtgtct tcaagtaacc ttgtaaggcg agaaagaaga
    35881 gggactggac aagaagtaag actaggaatt ctaagatagg ggatttgcca agttgaagaa
    35941 acttgctttc aaaaaccagt agtagggttt gtagtaggat gtagaaggat tcaattccca
    36001 aaatactagg cgtcaggaag cgattttccg tcagggtttg aaaactaatg gtcgaaatcc
    36061 cagtcgcgat ggctaccaag agataaacga tgatcttttg ggaacgcaac ttccaagcaa
    36121 aggctgacaa gtgagtgatg ggccaaaagt agagaagaca agctccgatg gcaagaataa
    36181 tgagaatcca gaagagcttg gtatgtttgc ttttagtctg catcttttcg tccccctctc
    36241 cagagaagta ggataaagac gagactaccg atgattccta gcaagagact gacagacaac
    36301 tcatagggcc taatcagaac tcgggatagg atatcgcaag ccagaactag attggcacca
    36361 accagtgcga ccatgagttt ggtttgactt agattatctc catagcgctt gcgaacaaga
    36421 ttgggaacga taactccgag aaatggtagg ccacccacgg taatcatggt gacgcttgtc
    36481 gttagcgcca ccagaaagag ggccagtttt tcaagtaggg agtaggaaat ccccaaactc
    36541 tcgctggttt ctttccctag attcatgatg gtgaaggttt gggataattt ccaaacggtt
    36601 atcaggatga tgaggcctaa gaagagccac tcatactgat gggtctgaat catggagaag
    36661 gagccctggg tccaggcagt catactctga accagattga aacgataggc gataacttct
    36721 gtgactgagc cgataatccc gctatagatg atcccaatca gaggcaacat ccacctttcc
    36781 tttacagtaa aaatggtcat aaaggctagg aagaagaggg tgaatacgat ggatgaaaca
    36841 aaagcgaaga gcatcttttg ggtcagacta gccgatggaa agacaaaaag gctcagcacc
    36901 attcccagtt tggcggcttc agtcgttcca actgtactcg gtgcagcaaa ctgattttgg
    36961 gtaatagtct gcatgagaag gcctgccata ctcatactag aggcagtcag gagaatactg
    37021 atagttcttg ggagacggga ctcttgaaag aggagccagg tctgctggtc gaaatcaaat
    37081 agctttcccc atgaaaaatc actggtccca atgctaatag agagaaagac taggagtaga
    37141 agtaagccaa ttaaataatg agaaagtttc ataccccgtc ctttcatgta gatttggtat
    37201 cgaaagatat ctgcggatat aaatgtaaca ttatttttct aatctgtcaa taaaatttct
    37261 gacaatttaa taaatacaac aaggagagag caacaagact ttctcctttg ttatcctatt
    37321 ctaaaatgtt tttaccttaa tctgataaaa taatatcttc gagggagtag ctagccgtcc
    37381 aatcaagata ttgtttagct tttgaagcat ctgctaggac actggctggg tcactagcac
    37441 gtcgagcaac aatctcgtgt gggatttttt aatttagtaa ttcttcagca gttttaaaga
    37501 tttctttgat agtatagcct tttttagttc ctaagttaaa gatttgagaa gaactgtctt
    37561 cttgaaatag gtagttcatt cctttaacat gagcctatgc aaggtccaag acataaatgt
    37621 aatctcgaat acatgaaccg tcacgtgtat cgtagtcatc tccaaatatt tttaagctat
    37681 catgttgtcc caatgcggtc ttgttgatat ttggaatgat gtgagttgga tttttcacac
    37741 gcagaccgtt tgaagcatcc atttcagccc cagcaacatt aaagtaacgg aaaataacat
    37801 atttccagtc gtagcgattg gccatccagt aaatcattcg ttcgcccatc agttttgtct
    37861 ctgcataagg gttgacaggg tcgagcaggg tatcttcagt caccggcttg tcaatacagt
    37921 tatttccata gagagaagca gtcgaagaga acatgatttt ttgaatgcca acttcagata
    37981 agactttgag aacttggttc ataccagcaa cgttggcagt gaagtattta cttggatttt
    38041 caatactttc gctcacaaca atttcacctg cacaatggag gacagcatca atctgatttt
    38101 cttctaagta agcctttaag gcgctagcat cataaacatc cagttcttta aagctagcac
    38161 gactatccac agccgctcga tttcctgtgg agagattatc gaggacatgc acctgatagc
    38221 cagcatttaa aagagcttta acggtatggg agccaatgta gctagtccca cctgtaacaa
    38281 gaattgtttt ggtcatgatt ttttcctttt ctagttttgt actatagtaa attgaaataa
    38341 gatatgaaca actctattag gaaagtcaaa ttaatttcta gaaatgtttc agcagctaca
    38401 gcgtactatt ccaaactcaa ccaactatat atttaaagaa agaagaaagg gaagtcaact
    38461 attttctgta aatttcatga aaaatccaaa caactttgat ggtgtattgt ttttttgttt
    38521 ggaaataaat attcgagtta attttggaat gtttactaga ttttgcacta catgcaagag
    38581 aatactatcc ttttctaaga tagagcagga ctaagatact aacttagaaa tccaccttcc
    38641 cactatcctt ctcctataaa aagtggtaaa atggatgaaa gaaagaagga actgaaatga
    38701 caacattatt ttcaaaaatt aaagaagtaa cagaacttgc tgcagtctca ggtcatgaag
    38761 cgcctgtccg tgcttatctt cgtgaaaagt tgacaccgca tgtggatgaa gtggtgacag
    38821 atggcttggg tggtattttt ggtatcaaac attcagaagc tgtggatgca ccgcgcgtct
    38881 tggtcgcttc tcatatggac gaagttggtt ttatggtcag cgaaatcaag ccagatggta
    38941 ccttccgtgt cgtagaaatc ggtggctgga accccatggt ggttagcagc caacgtttca
    39001 aactcttgac tcgtgatggt catgaaattc ctgtgatttc aggttctgtt cctccgcatt
    39061 tgactcgtgg aaagggggga ccaaccatgc cagccattgc cgatatcgtt tttgatggtg
    39121 gttttgcgga caaggctgag gcagaaagtt ttggcatccg tcctggtgat accatcgtac
    39181 cagatagttc tgcaattttg acagccaatg aaaaaaatat catctcaaaa gcttgggata
    39241 accgctacgg tgtcctcatg gtaagcgagc tagctgaagc tttatcaggt caaaaactcg
    39301 gcaatgaact ctatctgggt tctaacgtcc aagaagaagt tggtctgcgt ggcgctcata
    39361 cctctacaac caagtttgac ccagaagtct tcctcgcagt tgattgctca ccagcaggtg
    39421 atgtctacgg tggtcaaggc aagattggag atggaacctt gattcgtttc tatgatccag
    39481 gtcacttgct tctcccaggg atgaaggatt tccttttgac aacggctgaa gaagctggta
    39541 tcaagtacca atactactgt ggtaaaggcg gaacagatgc aggtgcagct catctgaaaa
    39601 atggtggtgt cccatcaaca actatcggtg tctgcgctcg ttatatccat tctcaccaaa
    39661 ccctctatgc aatggatgac ttcctagaag cgcaagcttt cttacaagcc ttggtgaaga
    39721 aattggatcg ttcaacggtt gatttgatta aacattatta aaccataagg gaggtggtag
    39781 ggatggtaga accaaaccta gaaagcctta taaaagatct ttacaatcat gctcgacatg
    39841 atttgagtga agatttagtt gctgctctcc tagagactac taaaaaactg cctactacaa
    39901 atgagcaatt gcaggcagtt cgtctctcaa gcctggtcaa tcgtgaattg ctcctaaatc
    39961 ccaaacatcc agcacctgag ttgctcaact tggctcgctt tgtcaaaaga gaagaagcca
    40021 agtacagagg aactgcgact tctgcgctta tgtatgagga actttttaaa atgctttgat
    40081 tccattgtgg atcaaagcat tttttataag acctatctcg tgagcgttca aatagtagta
    40141 acaaggaatc atgagaagtc agccgagacc atagtagtga agaaacttct gtaatggaag
    40201 tggagtgaag tgaggaatcg tcaaacggta ggggataggc tgtttgaagg gtgctacgaa
    40261 aaagataggg taaggtgaaa ttatagcgga atgaaataag atatgaacaa attgattagg
    40321 gaaatcaaat gaatttatag aaatctttta gcagtcatgg cgtcgtattc tagtttcaaa
    40381 atgctataaa tacgaacaaa ttgattggga aattcaaatc gctttctatc aatgttttag
    40441 aagatacagt gccctagtcc aacttcaata tactataaaa gaacagatgg aagcaattat
    40501 gatttacttc tatctttaaa ataaatgatc tgataggaaa aatgtgtccc ctcataaaaa
    40561 ataggatagt acggatggaa ggcttttgta gactggagga cgaaggaatt tagaaactct
    40621 cgttaaaagt tagttgagcc ggagtatggt ggtgtgaata attatcaaaa attatttcca
    40681 aatcgaaata gttgacagtt agaacgatga gtgttacaat tattttattc atttcgatat
    40741 cgaaataaat tggaggtgtt atgaaagata gtcatttgct agcccatcat attcgtttgt
    40801 tgaatgggcg gatttttcaa aagttactga gtcaagatcc tgaggctctt tataggggtg
    40861 aacagggcaa gattttagcg gttttatgga atagtgaaac tggctgcgca actgcgacag
    40921 atatcgcgct tgcgactggg cttgcgaata atacgctgac gactatgata aaaaagctag
    40981 aggaacaaaa gcttgtaatt gttagtccgt gtggaaaaga caagcgtaag aagtatttag
    41041 ttttaacgga gttaggcaag ttccagaaag aagtggggca tcgtgtcagt cagaaattgg
    41101 atactatctt ttacaaagga ttttcagagg aagaaattca ccaatttgaa ggttttcaag
    41161 aaagaatttt ggcaaatctg aaagagaagg aaaatgaggt ttagaatatg tcacaacaag
    41221 tgaaaaatgc tcacaaccta tacatccatg ccattcaaga cggacgagtt gctgaggctc
    41281 aagcccagtc tgtaggggat acctatatcc aacattcgac aggtgttcct gacgggaaag
    41341 aaggatttgc agccttcttt gcagatttct ttgagcgtca tccagagcgt cagattaaga
    41401 ttgtccgcac cattgaggat ggcaatctgg tctttgttca tgttcatcaa tatctgaatg
    41461 gtggagaagc tcaatgggtg acgacggata ctttccgtgc ggatgagaat ggtcgtattg
    41521 ttgagcattg ggatgtcatt gactactatc gaacacctga aaatgaccaa ctagatcaaa
    41581 tttttggaga ttttgaaatc aaggatttgg acaagaaagc agaaaataaa aagctggttc
    41641 gccgtttctt gacagaaact ttccaaaatg gggagctaga gcagtggagt gattatgtgg
    41701 cagacgattt gattcagcat aatcatgaga ttggacaagg aagtgctgct tataaaaact
    41761 atgtggctga atatagtgtc acttttgact ttgttttcca actcttagga caaggaaact
    41821 atgtggttag ctatggtcag actcagattg atggcgttgc ttatgcccag tacgatatct
    41881 tccgtttaga gaacgggaaa attgtggagc attgggataa taaggaagtc atacctaagg
    41941 tagaagactt gactaatcga gggaagtttt aaattgagga caaagaatga ttgaatacaa
    42001 aaatgtagca ctgcgctaca cagaaaaaga tgtcttgaga gatgtcaatt tacagattga
    42061 gaatggggag ttcatggttt tagttgggcc ttctgggtca ggtaagacga ccatgattaa
    42121 gatgattaac cgtctcttgg aaccaactga tggaaatatt tatatggatg gcaagcgcat
    42181 caaagactat gatgagcgtg aacttcgtct tactactgga tatgttttac aggctattgc
    42241 tctctttccc aatctaacag ttgaggaaaa tattgctctc attcctgaaa tgaagggttg
    42301 gactaaggaa gaaattgctc aaaaaacaga agagcttttg gcaaaagttg gattaccagt
    42361 agctgagtat gggcatcgac ttccaagtga gctatctggt ggagaacagc aacgggtcgg
    42421 tattgtccga gctatgattg gtcagcccaa gattctcctc atggatgaac ccttttcagc
    42481 cttggatgct atttctcgca agcagttgca ggttctgaca aaagaattac ataaagagtt
    42541 tgggatgaca acgatttttg tgactcatga tacggatgaa gccttaaagt tggcagatcg
    42601 tattgctgtt ttgcaggatg gagagattcg tcaggtgtcg aatcctgaga ctattttaaa
    42661 agctcctgca acagactttg ttgcagactt gtttggaggt agtattcatg actaatttaa
    42721 ttgcaacttt tcaggatcgt tttggtgatt ggttgacagc cctatctcaa catttgcagt
    42781 tgtcactttt gaccttgtta ctagctattt tgcttgcgat tcccttggct gtttatcttc
    42841 gctatcatga gaagttggcg gattgggtct tacagattgc agggattttc cagaccatcc
    42901 cgtctctggc cttgttggga ctctttattc ccttgatggg aattgggact ttaccggcat
    42961 tgacagctct agtgatttat gcgatttttc cgattttaca aaatactatt actgggctga
    43021 agggaattga tccgaacctg caagaggctg ggattgcctt tgggatgacc agatgggagc
    43081 gtctcaagaa gtttgaaatt ccacttgcca tgcctgttat catgtctgga attcggacgg
    43141 cagcggtctt gattatcggt acggctacct tggcgacctt gattggggca gggggactgg
    43201 gttcctttat cctgttggga attgaccgta ataatgccag tttgattttg attggggccc
    43261 tttcttctgc agtgctagct attgccttta acttcctact aaaagtaatg gaaaaagcga
    43321 aattgcggac gattttctct ggttttgcct tgatggcttt attactgggt ctgtcttata
    43381 gtccagccct tttggctcaa aaagagaagg aaaacttgat tattgctggg aaaataggcc
    43441 cagaaccaga aattttggct aatatgtata aattgctgat tgaagaaaat accagcatga
    43501 ctgcgactgt taaaccgaat tttgggacga caagtttcct ttatgaagct ctgaaaaaag
    43561 gtgatattga catttatcct gaatttaccg gtacggtgac tgaaagtttg cttcaaccat
    43621 cacccaaggt gagtcatgag ccagagcagg tttatcaagt ggcgcgtgat ggcattgcca
    43681 agcaggatca tctagcctat ctcaaaccta tgtcttatca aaatacctat gctgtagctg
    43741 ttccgaaaaa gattgctcaa gaatatggct tgaagaccat ttcggatttg aaaaaagtgg
    43801 aagggcagct gaaggcaggc tttacgcttg agtttaacga ccgtgaagat ggaaataagg
    43861 gcttgcaatc aatgtatggt ctcaatctca atgtggcgac tatgcagcca gctcttcgtt
    43921 atcaggcaat tcagtcaggg gatattcaaa tcacggatgc ctattcgact gatgcggaat
    43981 tggagcgtta tgatttacag gtcttggaag atgacaagca actcttccca ccttatcaag
    44041 gggctccact catgaaagaa gctcttctca agaaacaccc agagttggaa agagttctta
    44101 atacattggc tggtaagatt acagaaagcc agatgagcca gctcaactac caagtcggtg
    44161 ttgaaggcaa gtcagcaaag caagtagcca aggagtttct ccaagaacaa ggtttgttga
    44221 agaaatgatg ggacagagac tttaaacata ctgaactcaa cttccttaaa cgactagata
    44281 aaagaatgct cagacaattt cgtctgggct tttttgatgt gagaatgact gaatgtcact
    44341 taaaatggaa agaaagagaa aattttcatg attttctaaa attttactgt aaagacagtt
    44401 gactattcat aaaataggtg tataatattt tgtgaactac ttaacaaata agaggtttca
    44461 gctcatgtcg actaaggtta gaaatcttgt gtataggcag ttcattatct aatagagcgt
    44521 tgcgtccgaa agtctatcca gacacggctc tttaaaaaca aaaggagaaa tgatgcatac
    44581 ttatttgcaa aagaaaattg aaaatatcaa aacaacccta ggtgaaatgt caggtggtta
    44641 ccgtcgtatg gttgcggcta tggctgattt aggattttca ggaactatga aggctatctg
    44701 ggatgacctc tttgcccatc gtagttttgc ccagtggatt tatttgctgg ttttaggaag
    44761 ttttcctctc tggctggagt tggtttacga acatcgtatt gttgactgga ttgggatgat
    44821 ttgtagcttg acagggatta tctgtgtaat ctttgtatcg gaaggtcgag caagtaatta
    44881 tctttttggc ttgattaact ctgttattta ccttattttg gccctacaga aaggctttta
    44941 tggtgaggtg ctgacgacac tttacttcac agtcatgcag ccaattggac ttctagtttg
    45001 gatttatcag gcacagttta agaaggaaaa gcaggagttt gtcgcgcgta aactggacgg
    45061 caagggctgg acaaagtatc tttccattag tgtgctttgg tggttggcct ttggcttcat
    45121 ttatcagtct attggtgcca atcgtcccta tcgtgattca atcacagatg caaccaatgg
    45181 ggtagggcaa atcctcatga cagctgttta ccgtgaacag tggatattct gggcggctac
    45241 caatgttttt tcaatctatc tctggtgggg agaaagcctg caaattcaag ggaaatatct
    45301 aatttatctc attaacagtc tagttggttg gtatcaatgg agcaaggcag ctaagcagaa
    45361 tactgattta cttaactagg aaaagatgtt tgaaagtgct gttttgagat ttcgattaaa
    45421 acagatatag ttgataatca aggatttata gtatgaaaaa gaggatcggc gggtcctctt
    45481 ttgttgttga aaagataaaa aactcagtaa cctagaaata agacaactga gctttactct
    45541 atattcaatt tttaggaatg agaaggtcta gataaaattg gacaacttcc tggtctgtga
    45601 aatcttgacc ttttttgagc caccaggtca atgtctcgat aaagttggac atgaccaagt
    45661 gttggaggta agaagtaggc agattagggt gggcttcttt taaattatca gctagcacgg
    45721 aatagacgtg gtgctcaagc tctctctgga gttgacggag gaagtagtca tttttggaaa
    45781 atagcagact ggtgatatgg tcttggtttt tatgaaaatg gagaaagagg tgggcgaggt
    45841 agtcctcggt tgaaatggct tgctctcttt caaaaagatg atggaagagg tagcggcaga
    45901 gctgatccag aagaagctct ttactctcat agtgacagta aaaggtggat cgtcccacat
    45961 ctgcgagatc aatgatatcc tgaacagtag tggtctcgta gtccttagca ttcaaaagtt
    46021 gtataaaagc ttgatagatg gcttttttgg ttttgctgat acggcggtca atgttagtca
    46081 tatggacact tgaggcaaat tgttcagaac tgaataaagc tgacgttttg cttctatcct
    46141 ttctttgagt tttagtggat aatgataatg aacaaggtgt tcataaatct attataacaa
    46201 aggaatgaga aatatgaagg caaaatatgc tgtttgggtg gcttttttct taaatttgac
    46261 ttatgccatt gttgagttta ttgcaggtgg agtatttggt tctagcgctg ttcttgctga
    46321 ctctgtgcat gacttgggag atgcgattgc aattggaata tcagcttttc tagaaacaat
    46381 ctccaatcgt gaagaagaca atcagtacac cttgggctat aagcggttta gcctgctagg
    46441 agccttggta acagctgtga ttctcgtaac gggctctgtt ctagtcattt tggaaaatgt
    46501 cacgaagatt ttgcatccgc aaccagtcaa tgatgagggg attctctggt taggaattat
    46561 tgcgattact atcaatctgt tagcgagtct ggtggttggt aagggaaaga caaagaatga
    46621 gtctattctg agtttgcatt ttctggaaga tacgctaggg tgggtagctg ttatcctgat
    46681 ggcgattgtt cttcgattta cggactggta tatcctagat cctcttttgt cccttgtcat
    46741 ttctttcttt attctttcaa aagcccttcc acgtttttgg tctacactca agattttctt
    46801 ggatgctgtg ccagaaggtc ttgatatcaa gcaagtaaag agtggcctgg agcgattgga
    46861 caatgtggcc agccttaatc agcttaatct ctggactatg gatgctttgg aaaaaaatgc
    46921 cattgtacat gtttgtctaa aagaaatgga acatatggaa acttgtaaag agtctattcg
    46981 aattttccta aaagattgtg gttttcaaaa tattaccatt gaaattgatg ctgacctaga
    47041 aactcaccaa acccataagc gaaaggtgtg tgacttggaa cggagttatg agcatcaaca
    47101 ttagaaaaaa gtgaaaaata cttgggtact atcttatttg gaatagagta atttctttat
    47161 tatttaaata tttcaaaaat tggtaagaga agagcattgt ataaactcca gatatatgat
    47221 tgttaatgat aaaaattttt cgattagata caaaatgctt gacttggagt caactcaaag
    47281 ttatataata agataagtga gttagaatag cgtgaattca gtgaatgaaa tgagaggagg
    47341 ttagcgtgtg aatattaaat ctgccagtga tttgttggga atttcagcgg atacgattcg
    47401 gtattatgaa cgggttggtc ttgtgccacc gattactcgt actgctactg ggattcgtga
    47461 ttttcaagat caggatatcg aagcgctgga atttattaag tgttttcgtt cggcgggtgt
    47521 ctctgtagat agtttagttg actatatgtc gctctaccaa aagggagatg aaacgagaga
    47581 ggagaggctt ggtattttag aagaggaaaa gcaaaaatta gaggagcgct tgtctcagct
    47641 acagacagct ttaaatcgtt taaatctcaa aattaaactt tataaggaag gaaaatttta
    47701 aatgaaatca gcagtatata caaaggcagg tcaggttgga cttgcaagca ttgaacgtcc
    47761 gcaaataata gaagcggatg atgtgattat tcgtgtggtt cgtgcgtgcg tttgtggttc
    47821 agatttatgg aggtaccgta atccagaaac gaaagctgga cacaaaaata gtggacacga
    47881 agcgattggg attgttgaag aagctgggga agccattacg acggtgaaac caggtgattt
    47941 tgtgattgtc ccttttacac atggatgtgg tgagtgtgat gcctgtcttg ctggatttga
    48001 cggttcttgc gacaatcata ttggcaataa tttggggggt gattttcagg cagaatatat
    48061 tcgcttccac tatgcaaact gggcgctggt taaaatccct ggtcaacctt ctgactatac
    48121 agaagggatg ctcaagtccc ttttgactct tgcagatgtc atgccgacag gctatcatgc
    48181 ggcgcgtgtt gcaaatgttc aaaaagggga caaggttgtt gttatcggtg atggggctgt
    48241 tggtcaatgt gctgtcatcg cggctaagat gcgtggagca tcacaaatta tccttatgag
    48301 ccgtcatgaa gaccgtcaaa agatggctat ggagtcaggt gcgacagctg ttgttgcaga
    48361 acgtggtcaa gaaggaatta ccaaggtgcg tgaaatcctc ggtggaggag cagatgcagc
    48421 acttgaatgt gttggtacgg aggctgctat agaacaggcg ctaggtgttc ttcataatgg
    48481 agggcgtatg ggctttgtag gagtcccaca ctataataat cgtgctcttg gttcgacatt
    48541 tatgcaaaat atctctgtag caggtggggc agcttctgct acaacatacg ataagcaatt
    48601 tttactaaaa gccgtccttg atggtgatat caatccaggt cgcgtcttta cttcaagtta
    48661 taaactggaa gatatcgacc aagcctacaa agatatggat gaacgtaaga ctattaagtc
    48721 tatgattata atcgaataaa aaacgaatag gagttctaaa actcctattc gttttttatg
    48781 ttatcctatt cttgatttag ggtactttct cttaatgtca gtttggttcc caacatggtc
    48841 aggctaggga ttttccgacc gtggaggact tccttgttaa gaatatccat acctgctcgc
    48901 cccatttctt cagtataaac tgtgatgctg gagaggggag gataaacctg cttggtcagg
    48961 ctggtgtcgt taaaggaaat gaggctgaca cggtctggta ggctgattcc agcttctttg
    49021 agcgcacgaa gggcaccgat ggctaaacta tcgctggctg cgaaaaatgc tggtgggagt
    49081 tggtctccca agttctgaat ggcttccttc atcaagtcat agccagactg ggcagtaaag
    49141 tttccttgaa agatcagttc atcatgatag attccttttg cttgactgta gtttctgaaa
    49201 ttttccagcc gcttatcctc aatgatttct tcttggtcag ttgtttcttc aaggcctgtt
    49261 agaatcccga tacggtccat tccttggctg aggaaataat cgacaacctg tttcatagca
    49321 gtgtaaaaat ccgtgataat acaggtatgt cccagggaaa gtgtatcgct gtctagaaat
    49381 acaagaggct tttggtattc ttcaaaggca gaaatctgag ctcgactaaa ctttccgatg
    49441 cagagaatcc caatcacttc ctcgcttaag gtaaaaggat ggtcattaaa atagcgcaag
    49501 atatcatagt ccaactcttg ggctcttttt tctattccta ggcgaatctg gtagtagtag
    49561 aggtcgtcca gctccccttg ttcgctgacc cattgaataa tggcaatctt ttgcttgggt
    49621 ttatgggact cgcctgtctt gaggtgcttg gtgtagccca gctcttcagc aacggttaaa
    49681 atacggtgtc tggtttcttc tgtaacagat aggctctggt cgcggttgag gacgcgggat
    49741 acggtcgcga tagagacaga ggctagctgt gcaatgtctt ttaaggtagc cataaatcct
    49801 ccttgattag gttagtatat catatttttc ttctttttac tgatagttta ctaaaatttt
    49861 agtaaaaagg attgaccttg ggaaattcct tggatacaat agaagaaaac gattacacgt
    49921 taaggtggct taacggacag tcaaaggaga aatcatatga cacaacatct tactgctgaa
    49981 actcttcgca aagactttct tgctgttttt ggtcaagaag cagaccaaac tttcttttca
    50041 ccaggccgta ttaatttgat tggtgaacac acagactaca acggtgggca cgtttttcct
    50101 gctactattt ccttgggaac ttacggtgca gctcgtaagc gtgacgacca agttttgcgt
    50161 ttctactcag ctaactttga ggacaagggc attatcgaag tgcctctcgc tgacctcaag
    50221 tttgaaaaag agcacaactg gaccaattat ccaaaaggtg tccttcattt cttgcaagaa
    50281 gctgggcacg tgattgacaa aggttttgat ttttatgttt atgggaatat cccaaatggt
    50341 gctggtttgt cttcttcagc atccttggag ctcttgacag gggtagtggc agagcatctc
    50401 tttgatttaa aattagagcg tttggatttg gttaaaatcg gaaaacaaac agaaaataac
    50461 tttatcggag tcaactctgg tatcatggac cagtttgcta ttggtatggg ggctgatcaa
    50521 cgtgctattt accttgatac caacacctta gagtatgatt tggtgccact tgatttgaag
    50581 gacaatgtcg ttgttatcat gaacaccaac aaacgccgtg aattggcgga ttctaaatac
    50641 aatgaacgtc gtgctgagtg tgaaaaagca gtggaagaat tgcaagtttc cttggatatt
    50701 cagactctgg gtgaattgga cgagtgggcc gttgaccaat atagctatct gattaaagat
    50761 gaaaatcgtt tgaaacgtgt tcgccatgct gtgcttgaaa accaacgtac cctcaaagct
    50821 caagtagcac tccaagcagg agatttggaa acatttggac gcttgatgaa tgcgtcacac
    50881 gtttctctgg agcatgatta tgaagtaact ggtttggaat tggataccct tgttcacaca
    50941 gcttgggcac aagaaggagt tctcggtgct cgtatgacag gggctggttt tggtggctgt
    51001 gccattgcct tggttcaaaa agatactgtt gaggccttta aggaagctgt aggcaaacac
    51061 tacgaggaag tagttggata cgctccaagc ttctatatcg ctgaagttgc aggtggcact
    51121 cgcgtccttg actagtcaaa aggaggctct atagtgacct tagtagataa atttgtaaca
    51181 catgtcattt ctgaaagctc atttgaggaa atggatcgaa tctatctgac caatcgtgtt
    51241 ttggcacgag tgggagaagg tgttttggaa gttgagacca atctggataa attgattgac
    51301 ctcaaggacc agctggttga agaagccgtt cgattagaga cgattgagga tagtcagact
    51361 gcgcgtgaaa tccttggtac tgaactgatg gacttggtga ctccttatcc gagtcaggtc
    51421 aatcgtgact tctgggaagc ctatgtccac tctcctgagc aagcgataga ggatttttat
    51481 caactcagtc agaaaaatga ttacatcaaa ctcaaggcca ttgctaaaaa tatcgcttat
    51541 cgtgttccat ctgattatgg agaacttgaa attaccatca atctctctaa gcctgaaaag
    51601 gatccgaaag agattgcggt agccaagttg gtgcaagcta gtaattatcc tcagtgtcaa
    51661 ctttgtttag agaatgaggg ttaccatggt cgggtcaacc acccagctcg cagcaaccac
    51721 cggattatcc gttttgaaat ggttggtcag gaatgggggt tccagtattc gccctatgct
    51781 tactttaatg agcattgtat ctttttagat ggccagcatc gtcccatggc cattagtcgt
    51841 cagagttttg aacgtctgtt ggctatcgta gagcagtttc caggatattt tgctggatct
    51901 aatgccgacc tgccgattgt ggggggctct attctaactc atgatcatta tcagggaggc
    51961 cgtcacgtat ttcctatgga attggctccc ttgcaaaaga ccttccgatt tgctggtttt
    52021 gagcaggtca aggctggaat tatcaagtgg ccaatgtctg tcctacgctt gacttcggat
    52081 tccaaagagg atttgattaa cttagctgat aagattttcc aggaatggcg tcagtattca
    52141 gattctagtg tgcagatttt ggcagagaca gacgggacac cgcatcacac catcacacca
    52201 atagcgcgta agcgtgatgg acagtttgag ttggacttgg tcttgcgaga caatcagact
    52261 tcagcagagc atcctgatgg catctatcat ccccacaagg atgtccaaca tatcaagaag
    52321 gaaaatatcg gcttgattga ggtcatgggc ttggcaatct tgccaccacg tctgaaagaa
    52381 gaagtagagc aagtcgctag ctatcttgta ggagaagctg ttacagttgc cgattatcat
    52441 caggagtggg cagaccaact caaatcccaa catccagacc taacggataa agaaaaagcc
    52501 cttgcaatcg tcaaggactc tgtgggtgct atctttgtgc gtgtacttga ggatgcagga
    52561 gtctacaagc agacagaaca agggcagaca gcctttatgc gctttgtgga acaggtcgga
    52621 attttactag actaggagct ttctccttgc acagtcctat aaaaagtagt atcatagtgt
    52681 cgtttgaaat atcaggagga aacgatgaaa gactttcatt ttgacgctat atctgccttt
    52741 gaaaattacg aaattgaaaa aatgagagat ggtcacgttg tggtgacgac caaagtagtg
    52801 aactcatcgc tcaactacta tggcaatgcc catggtggct atctattcac gctatgtgac
    52861 caaatcagtg gtttggtggt tatctcgctg ggacttgatg gggtgacact ccaatcctct
    52921 atcaactacc ttaaggcagg aaaactcgac gatgtattga ccattaaagg agaatgtgtc
    52981 catcaaggtc gtacaacctg tgtgatggat gtggatatca ccaatcaaga gggcagaaat
    53041 gtctgtaagg caacctttac catgtttgtc acaggccaaa ggtctgagga aagacgggta
    53101 agtatataaa gaaaggtgag tgggataaat ttatctcact catttttaat ttatgagata
    53161 tgttagaagg aaatataaaa gagagtgatg aaatacaaat gtaataggta catatatgtg
    53221 gtatactagt tatagtagat tttaattgct tatatcgagt gagttctcat attagtgagg
    53281 aatttttaaa tggaattaca ctttaattta gaattagtag aaacctataa aagtaatagc
    53341 caaaaggcac gtatcttaac ggaggattgg gtttatcgtc agtcttattg ccctaattgt
    53401 gggaacgatc ccttaaatca ttttgaaaat aatcggcctg tagcagattt ttactgtaat
    53461 cattgtagtg aggagtttga actaaagagc aaaaaaggaa atttttcatc aacaatcaat
    53521 gatggtgctt atgcaacgat gatgaagcgt gtgcaggcag ataataatcc taatttcttt
    53581 tttttaactt acacaaaaaa ttttgaggta aataactttc ttgtccttcc gaagcaattt
    53641 gttacaccga aatcgattat tcaaagaaaa ccacttgcac caactgctag acgagcaggt
    53701 tggattggtt gtaacattga tttatcacaa gtaccttcta aaggaaggat atttcttgtg
    53761 caagatggac aagttagaga tccagaaaaa gttacaaaag aatttaagca aggtttattt
    53821 ttaaggaaga gctctctgtc atcaagaggt tggacaatag aaattctaaa ttgtatagat
    53881 aagatagagg gttcagaatt tacccttgaa gatatgtatc gttttgaaag tgacctaaaa
    53941 aatatctttg ttaagaacaa tcatatcaaa gaaaagatta ggcaacagct tcaaatatta
    54001 agagacaaag aaataataga atttaaaggt agaggaaagt attggaaatt atgaaaacga
    54061 aacaacttgt tgcatcagaa gaggtgtatg atttcttaaa agtcatctgg cctgattatg
    54121 aaactgaaag ccgttacgat aacctaagtt taatcgtctg taccttatca gatcccgatt
    54181 gtgtgagatg gttatctgaa aatatgaaat ttggtgacga aaaacaacta gctttgatga
    54241 aggaaaaata tgggtgggaa gtaggagata aattgccaga gtggctacat agctcctatc
    54301 atagattatt gttaataggt gaattattgg aaagcaatct aaaactgaaa aagtatacag
    54361 tagaaattac agaaacttta tcacgtttag taagtataga ggctgaaaat ccagatgaag
    54421 ccgaacgact tgtaagagaa aagtataaga gttgtgaaat tgttcttgat gcagatgatt
    54481 ttcaggacta tgacactagc atatatgaat aggtagatgt ttttattttg tcacacaaaa
    54541 aagaggctcg cacctctttt tcttatttct ttttatgatt taatacggca ttgaggacaa
    54601 tagcgagtag gctggctacg acgattccgt ttgagaagaa catttggaag gctgtcggca
    54661 tgctgacaaa gagattactg ttgttgagac cgacacctgc agcgattgaa acagctgcga
    54721 taaggaagtt gtgttcattg ttagcaaagt caactcgggc gaggatttgc atcccttgaa
    54781 tagatacaaa accaaacatc accagcatgg caccgccgag gacggagctt ggaatgattt
    54841 gggcaagggc accaaactta ggaagcagtc caaggagaac caggaaacca gctgcgtagt
    54901 agattggcag gcgttttttg atgcctgaca atttaaccaa accaacgttt tgtgaaaatc
    54961 cggtgtaagg gaaggtgtta aagattcctc cgagaagtac cgccaaacct tctgcgcggt
    55021 aaccgttgcg aaggcgcgtg ctgtcgattg gatcttttgt gatatcagac aaggctagat
    55081 aaacaccagt tgactcaacc atagacaccg ttgcgatgat acacatcatg gcaatagatg
    55141 agatttcaaa ggttggcatc ccaaagtaga gtggagttgg gacatggaca agtggagcta
    55201 ccgcaacagg agagaagtcc accaagccca tagtagcagc aatggcagtt ccaacaacca
    55261 gaccaatcaa aatagagata gacttgataa atcctttggt aaagatgttg atcaagagga
    55321 taatcagaac agtaatagct gaaagcaaga gactttgacc agttggctct ggaacgttat
    55381 ttcccatatt tccaatagcg acagggatca aggttaaacc aatcgtggta ataacagatc
    55441 ctgttacgat agatgggaag agattggcta cttttgagaa gatgcctgaa acaagaacca
    55501 cgtaaatccc agatgcgata agggcaccaa acatagcgcc actaccatgg ctttgcccaa
    55561 tcataatcaa gggagcgacc gactggaatg caactccaag aacgactggg agtccaatcc
    55621 caaagtattt gttgagttgg agttggagga aggttgccac cccacacatg aagatatctg
    55681 tagaaatcag gtaggtcaac tgctcagctg aatagccaag ggctgtcgca atcatgatgg
    55741 gaaccaggat agatcctgag tacatggcta gtaagtgctg caagcccaga acggctgctt
    55801 gcgagtgttt ttcttgagtt tgcattagag atctgcctcc ttaaatacga cctgaccatt
    55861 ttcaaaacga tccaagcgag caagtgatag gacagggtag cctgcttttt caagcaaatc
    55921 acgaccatct tggaaggatt tctcaatcac gataccgata gcttggactg tggcaccggc
    55981 ctgttcgatg atttgaatca agcctttagc agcttggcca ttagcaagga aatcgtcgat
    56041 aatcaaaacc ttgtcctctg gtgagaggaa ttttccagcg atagaaacgg tgctggtcac
    56101 ctgtttggta aaggagtaga cttgagcagt taagatgcct tcattcatgg tgatgttctt
    56161 agcttttttg gcgaaaatca tgggaacgtt taaggcttca gctgtaaaaa cggctggggc
    56221 aatacccgac gcttcaatgg ttacgacctt ggtaatgcca gcagcagcaa atttttccgc
    56281 aaaaacctta ccaatctctc gcatcaagct aaagtcaact tggtgggtta aaaaggaatc
    56341 taccttgagg atgttatcac ccaagatatg cccatccttg aggatgcgct cttctaataa
    56401 tttcataaga cctcctaaag tctaaaagtt aatttacttg ttgtttaaat atttctatag
    56461 tgatcccttt tgctaatact atatatttga taaaactatt acgagcgaag cgagtcttat
    56521 caaatatttc ccgttgtagt ggtatcatag acaataatct tgttattgtc tatgacggga
    56581 tttttgagag taaaatagtt cggggaacta ttttagccta agcctagaaa tgaaagagct
    56641 aggggctcaa aaattaggga tgaaattccc tggattcctg aaattattca caggataatt
    56701 tcacctcccg tccgcactaa ttaagggaaa tattaaaaat attcaaaaaa gacctactta
    56761 atctctaagt aagtccccta aatagacatg gcaaaaacgg ccatatctca ctgctgactt
    56821 acttattgtt aggtgttccg gcaccttgta gaaacgtcgt gccaattcac gacataaaca
    56881 agtaaaacga tattcaattt taaataggct tgagccaatg tttttatttt acactaaata
    56941 actttagaaa tcaactattt tgttagtgtt ttggtttaaa aagcgaacaa aaagaagaga
    57001 gggtgaacaa aaactccatt gtaagctaac atttatacta aatgaaaatc aaagagcaaa
    57061 ctaggaagct atccacaacc tcaaaacact gttttgaggt tgtggataga attgacagag
    57121 tcagtatcat atacctacgg taaggcgacg ttgacgtggc ttgaagagat tttcgaagag
    57181 tattagaaga tttttccatc ataaaaggca tactatcaag tttttagaca cctgacaata
    57241 tgcctttttc taactttaaa gacttttccc aatttttatt attctactcg ctaaatctta
    57301 aaaaatagcc atttggatcc aaaactgcaa attcatgagg atagatatag ggatcactga
    57361 cacgaaactt tctcttggtc aagggacgat aaataggata gtttgccttc atcactcttt
    57421 aatagagttt tgaaacatcc tttatgccaa aggagagatt gactccacga ccaaatggat
    57481 aggtcagttc agctagttga tcctttgttc cctcctctaa cattagttga cactcttcaa
    57541 gagaaagaga aagttttctt ctggacgttg gtattcaatc ctaaaaccca gtaaaccaca
    57601 gtagaaggac cgggactgtt cgatattcga tacaagcaac tcgggaatga ccgcattgta
    57661 gtccatatag aaaatcctta caagtcaatt tccaagacaa tcggtgtatg gtcttggcga
    57721 gcacctgagt caatcatatc agatttagtg accttgtcag cgatacggtt acttgtgagc
    57781 cagtagtcga ttctccagcc tgtattgttg attttagaag ttttgctgcg ttgtgcccac
    57841 caagtgtagc gttcaggaac atcgccatga acatggcgga aggtatcagt aaatccagtt
    57901 gccaataggt tggtaaatcc agcacgttcc tcgtcagtaa atccaggtga acggcggttg
    57961 ctagcaggat ttgcaaggtc gatttcattg tgggctacgt tgtagtcacc ggtcgcaagg
    58021 actggttttt ctttgtctag ttcagccaaa tactccgcat atttgacatc ccagacttgg
    58081 cgttcttcca agcgtttgag accgtcacca gcgtttggag tgtaaacttg ggttacgaaa
    58141 aatgcatcaa attctagagt gatgatacgg ccttccaagt ccatggtaga aggggcaccg
    58201 atttctggga agctgatcgt aggcgtaagt tctttcttat aaaggaacat ggttccagca
    58261 tagcctttac gggcaggctc ttgggaagag cgccacgtgt tttcgtagcc tgggaagagt
    58321 tcttctaaaa tttccacgtg tttctttgta ggtcctttgg cagaaagctt ggtttcttgg
    58381 atagcaataa tatcagcatt ttcagcgacc aaggtttgta ggacttcttg ggacaatttg
    58441 gcacgagctg agtcactagt tagggcagcg tttagggaat caatattcca tgagataagt
    58501 ttcataaagt tacctttttc attcagatta tagattttat tataccaaaa aaaggtctat
    58561 ttccccaacg tatggtttga aaaattactc tctttcgttt ataattaaga atgattttat
    58621 gaaagggagt gaaaatacat gaaattctac tcttatgact atgtactcag ccaaatcggt
    58681 cagcaaaatg gtatcatggt tggctttggg attgttttat tagctgtgac agtttttttg
    58741 ctttcaaggc ataccatgat aaaaaaagga atcaaatttc gtgagttggt catgatttca
    58801 gctctggcct tgtttagctc tgcttttggt cagcatcacg acttatcaaa acaatcaagt
    58861 ttctaacaat aaatttcaaa ctttacttca tttcatcgag gttgtttcca aagatttggg
    58921 agtagacaag tcagaagtct atgttaatac ttccacaaac acagatggcg cacttatcaa
    58981 ggtgggagat cgctattatc gtgccctaaa cggaagtgag ccagacaagt acctgttaga
    59041 gaaagtcgaa ttgtataaga cagacgcaat tgaactggtg gatgtgaaca aatgacactt
    59101 aattatatcg aaattttaat caaactgatc ttgactctca aatagctcaa caacaatgtt
    59161 cactttgtga aacgtttgat tgatggtaag ccaactctcc ttatcaaaaa tgggaatatt
    59221 gacccataag cctgtcgttc agttggtttg tctgcatcgg atgtagccct caaacttcgt
    59281 agccaaggga ttttccagat gaagcaagtc aaacgagctg tgcaagagca aaatgggcaa
    59341 ctcatcgttg tgcaaatggg agatgaaaat cctaagtatc cagttgtgac tgacggtgtg
    59401 attcaagtag acgtcttgga atcgattggt cgtagcgaag agtggttgct tgataacctc
    59461 agtaaacaag ggcatgacaa tgtagccaat atctttattg ctgaatatga caagggtgct
    59521 gttacagtcg taacttatga aaaagaaaaa cctggggtct tgtactcttc gaaaatctct
    59581 tcaaaccgcg tcaacgtcgc cttgccgtat gtaggttact gacttcgtca gttctatcta
    59641 caacctcaaa gcagtgcttt gagcagcctg cggctagttt cctagtttgc tctttgattt
    59701 tcattgagta ttggcctcag gtttccattt gcaatcagaa agggatttta tgtccattat
    59761 tcaaaaactt tggtggtttt tcaagttaga aaaacgccgt tatctagtcg gaattgtggc
    59821 cctggtcttg gtttccgtcc tcaatctcat tcctcctatg gttatggggc gggttattga
    59881 tgccatcaca tcggggcaat taacccagca ggacctcctt cttagcctat tttacttgct
    59941 acttgcagcc tttggtatgt actatttgcg ctatgtgtgg cgtatgtata tccttgggac
    60001 ctcttattgc ttgggacaga tcatgcggtc tcgcttgttt aagcatttca caaaaatgtc
    60061 gtcagccttt tatcaaacct atcggacggg tgatctgatg gcacacgcaa ccaatgatat
    60121 caatgccttg actcgtttag caggtggcgg tgtcatgtct gcggtggatg cctctatcac
    60181 ggctctggtg actttgttga ccatgctctt tagcatctca tggcaaatga ctcttgttgc
    60241 cattctcccc ctacctttca tggcctatgc gactagtcgc ctagggagaa agactcataa
    60301 ggcctttggc gaatcccaag ctgctttttc tgaactcaat aacaaggtac aggagtccgt
    60361 atcaggtatc aaagtgacca agtctttcgg ttatcaggca gacgagttga agtcttttca
    60421 ggcagtcaat gaattaacct tccaaaagaa cctgcaaacc atgaaatatg atagtctctt
    60481 tgaccctatg gttctcttgt ttgttggttc gtcctatgtt ttaacgcttt tggttggctc
    60541 cttggtggtt caggaagggc agattacagt tgggaatcta gtcaccttta tcagctattt
    60601 ggatatgctg gtctggcctc ttatggccat cggcttcctc tttaatacta ctcagcgagg
    60661 gaaggtttct taccagcgga ttgaaaatct tttgtctcag gaatctcctg tacaagaccc
    60721 ggagtttcct ctggatggta ttgaaaatgg gcgtttggag tatgccattg acagctttgc
    60781 ttttgaaaat gaggaaacac tgacggatat tcactttagt ttggcaaaag ggcaaacact
    60841 gggcttggtt gggcagacag gctctgggaa aacgtcctta atcaagctcc tcttgcgtga
    60901 atacgatgtg gataagggcg ccatttatct aaacggtcac gatattcggg actatcgtct
    60961 gacagacctt cgcagtctca tgggctatgt tcctcaggac cagtttcttt ttgcgacttc
    61021 aatcctagac aatatccgct ttggcaatcc taacttgccc ctttcagcgg tcgaggaagc
    61081 tactaagcta gcccgagttt accaagatat tgtagacatg cctcaaggat ttgatacgct
    61141 gattggtgaa aaaggagtca gtctttctgg tggtcaaaag caacggttgg ctatgagtcg
    61201 ggctatgatt ttagaccctg atatcttgat tttggatgat tccttatccg ccgtagatgc
    61261 caagacagag tatgcgatta tcgacaacct caaggagacg cgaaaggaca agacaaccat
    61321 tatcactgcc catcgcctga gtgcagttgt ccatgcagat ttgattttag tcctgcaaaa
    61381 tggtcaaatt atcgaacgag gcaggcacga agacttgcta gctttggatg gctggtatgc
    61441 ccaaacctac cagtctcagc agctggaaat gaagggagaa gaagatgcag aataaacaag
    61501 aacaatggac tgtattgaag cgcttgatgt cctatctcaa gccttatgga ctccttacct
    61561 ttttggcact cagttttctc ctagcgacga cggtcattaa aagtgtcatt ccccttgtgg
    61621 cttcccactt tatcgaccag tatctcagca atcttaacca actagccgtg accgttttgc
    61681 tggtctacta tggtctctac atcctacaaa ctgtagttca gtatgtcggc aatcttctct
    61741 ttgcgcgcgt gtcttacagt attgttaggg atattcgtcg ggatgccttt gccaatatgg
    61801 agaaactggg catgtcttac tttgacaaga cgccagcagg ttctatcgtt tctcgtttga
    61861 ccaacgatac cgagacgatt agtgatatgt tttctgggat tttatccagc tttatctcag
    61921 cagtttttat ctttctgaca accctttata ccatgttggt gctggatttt cgtttgacag
    61981 cattagtctt gctctttctc cccttgattt tccttttggt caatctctat cgaaaaaagt
    62041 cagtgaaaat catcgagaaa accagaagtc tcttgtcaga tatcaatagt aagctggcag
    62101 agaatatcga gggaatcagg attattcagg cttttaatca agagaagcgc ctgcaggcag
    62161 aatttgatga aatcaaccaa gagcacttgg cctatgccaa ccgttctgta gccttggatg
    62221 ccctcttttt gagacctgcc atgagtttgt tgaaacttct aggttatgct gtcttgatga
    62281 cctattttgg ctatcgtggt ctttctatcg ggataacggc cgggaccatg tatgctttta
    62341 tccagtacat caaccgcctc tttgaccctt tgattgaggt gacgcaaaac ttttcaacac
    62401 tgcaaacgtc tatggtttct gcaggccgtg tctttgcctt aatcgatgag aggacctatg
    62461 agccccttca agaaaatggg caagccaaag tcaaagaagg caatatccgt tttgaacatg
    62521 tgtgtttctc gtatgacggt aaacatccga ttctggatga catttctttc tctgttaata
    62581 agggtgaaac cattgccttt gtaggtcata caggttcagg gaaatcgtct attatcaatg
    62641 tcctcatgcg cttttatgaa ttccagtcag ggagagttct cttggatgat gtggatatca
    62701 gggatttcag tcaagaagag ctgagaaaaa acatcggttt ggttttacag gaacccttcc
    62761 tctatcatgg aactattaag tccaatatcg ccatgtacca agaaatcagt gatgagcagg
    62821 ttcaggctgc ggcagccttt gtggatgcag attcctttat tcaagaactt cctcaggggt
    62881 acgactcccc tgtttccgag cgtggttcga gcttctctac tgggcagcgc cagcttcttg
    62941 cctttgctag aacagtcgcc agccagccta aaatcctgat tttggatgaa gcgacagcca
    63001 atattgactc tgaaacagaa agcttggttc aagcttctct ggcgaagatg agacagggcc
    63061 gaacaactat tgctatcgct caccgccttt ctactattca agacgccaac tgcatctatg
    63121 tcttggataa gggacgcatt atcgagagtg gaacccatga ggaactcttg actctgggag
    63181 gaacctatca caagatgtat agtttgcagg caggggccat ggccgatact ctttgaaaat
    63241 ctctttaaac catgtcagct ttatctgcaa tctcaaagct gtactttgat tttcattgag
    63301 tactagaagg aaatccttca aattacagat ttctttcacc gccttttcca ttttgtggta
    63361 taatgaaaaa tgttgacaaa tagtataata aaaacaaagg agaacagtat gctgaaatgg
    63421 gaagacttgc ctgtggaaat gaaatcaagc gaggttgagt cttactacca tcttgtctct
    63481 aaaaggaagg gttcgctgat tttcaagcgt tgcttggact ggtttttggc cttggtctta
    63541 ctggttctaa cctctcccat ctttctcatc ttgagcattt ggatcaagtt ggatagcaag
    63601 gggccagtga tttacaagca agagcgtgtg acccagtaca accgtcggtt caagatttgg
    63661 aagttccgta ccatggtgac ggatgcggat aaaaaaggaa gtctggtgac ttctgctaac
    63721 gatagccgta ttaccaaggt tggaaatttc atccgacgtg tccgtttgga cgaactgcct
    63781 cagttggtca atgtccttaa aggtgagatg tcctttgtcg gtacacgacc tgaagtgcca
    63841 cgttatacag agcagtatag ccctgaaatg atggcaacct tgctcttgca agcaggaatt
    63901 acctctccag ccagcatcaa ctacaaggat gaggacacca tcatcagtca aatgacggag
    63961 aaaggtctgt cagttgatca ggcctatgtg gagcatgttc ttcctgaaaa gatgcgctat
    64021 aacctcgcct atctccgaga gtttagtttc tttggggaca tcaaaatcat gtttcaaacc
    64081 gtgtttgagg tactaaaata aagtagtcat aagaaaatga gtacagataa aaggagcaaa
    64141 tcaatgccaa attacaatat tccattttca ccgcctgata tcacagaagc agaaattgct
    64201 gaagtagcgg ataccctgcg ttctggttgg atcacaacag gtcctaaaac aaaagaactg
    64261 gagcgccgct tgtctcttta cacacagaca cctaagactg tttgtctcaa ctctgcgaca
    64321 gccgctctgg agttgatttt acgcgttttg gaagtgggac ctggtgatga agtcatcgtt
    64381 ccagccatga cctatacggc ttcatgtagt gtcattacgc acgtgggagc aacccctgtc
    64441 atggtggata tccaagcaga tacgtttgag atggactatg acctgcttga gcaagctatc
    64501 actgagaaaa ctaaggtgat tatcccagta gagctcgcag ggattgtttg cgattatgac
    64561 cgtttgttcc aagtcgtgga gaaaaaacgt gacttcttta ctgcttcaag caagtggcaa
    64621 aaggccttta accgtattgt cattgtctct gatagtgccc acgctttggg atctacttat
    64681 aaaggacaac cttctggttc tatcgctgac tttacttcct tctcattcca tgccgttaag
    64741 aactttacaa cggcagaagg tggaagtgcg acttggaaag ccaatccagt gattgatgac
    64801 gaagagatgt acaaggaatt ccaaatcctt tcccttcacg ggcaaactaa ggatgctctt
    64861 gccaagatgc aattggggtc atgggaatac gatatcgtta caccagccta taagtgcaac
    64921 atgaccgata tcatggcttc acttggtttg gtacaattgg accgctatcc aagtttgttg
    64981 caacgccgta aggacattgt ggaccgctat gatagtggtt ttgcaggttc tcgcatccat
    65041 cctttggcac acaagactga aactgtcgaa tcttcacgcc acctctacat cacccgtgta
    65101 gaaggagcaa gcctagaaga acgcaacctc atcatccaag aattggctaa agcaggaatt
    65161 gcaagtaatg ttcactacaa accgcttcct ctcttgacag cctataagaa tcttggattt
    65221 gatatgacga actatcctaa ggcctatgcc ttctttgaga atgaaattac cctccctctt
    65281 catactaaat taagcgatga agaagtagac tatatcattg agactttcaa aacagtttct
    65341 gaaaaagtgc taactttatc aaaaaaatga caaactacag tcaagcgaaa gtgatcctgt
    65401 ccctaaaaag tctaattgag tgtaaaaact gttgttttca attgataata gtttacacct
    65461 gtagttgagg ctcctttctc ctcagagaga gaatttttat aggattttcc tttcttgtgg
    65521 gagtcccgtg gtttgaaata agatgtgagc aatttggtgt agcatttaga atccttacta
    65581 gacatcattt agaaaatcta gtgtcttgtt ctagttttca attcacccta ttttttgaaa
    65641 gacgtgagtt tccatgagtg agattgtgga aactcgcgtc tttttttgtt ttcagaatat
    65701 tgttcaaagt tttgtgcctg tctttcatgt tctagtcatt cttttgcatg atagaattta
    65761 tagaatgtta atattataat aatacaaata ctctatatgt ttagtgatgc ttgcatctcc
    65821 tgcgagacaa tctataaaac acttgtctac gattacctat atgccctatt ccagtatttt
    65881 agaagcactg catctatttt tatcgaggtt aaatctagct tttatagaag gtctatttaa
    65941 gaaatatatt gtagtgtttt agtttcaatc cgccatatga gcgatattca tgtaaatatc
    66001 cctggcgaat gcttgtatga caagatattt gttctttcat ttataattta c
//
LOCUS       DAVMEI010000002        49722 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVMEI010000002 DAVMEI010000000
VERSION     DAVMEI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.002"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(134..592)
                     /locus_tag="VYQ24_000066"
     CDS             complement(134..592)
                     /locus_tag="VYQ24_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1967263.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(589..1029)
                     /locus_tag="VYQ24_000067"
     CDS             complement(589..1029)
                     /locus_tag="VYQ24_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1967264.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1235..3184
                     /gene="mutL"
                     /locus_tag="VYQ24_000068"
     CDS             1235..3184
                     /gene="mutL"
                     /locus_tag="VYQ24_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1967265.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3509..3976)
                     /locus_tag="VYQ24_000069"
     CDS             complement(3509..3976)
                     /locus_tag="VYQ24_000069"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1967266.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3977..5182)
                     /locus_tag="VYQ24_000070"
     CDS             complement(3977..5182)
                     /locus_tag="VYQ24_000070"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1967267.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5202..5837)
                     /gene="ribE"
                     /locus_tag="VYQ24_000071"
     CDS             complement(5202..5837)
                     /gene="ribE"
                     /locus_tag="VYQ24_000071"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1967268.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5822..6922)
                     /gene="ribD"
                     /locus_tag="VYQ24_000072"
     CDS             complement(5822..6922)
                     /gene="ribD"
                     /locus_tag="VYQ24_000072"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1967269.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNGAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7057..7171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7328..7921
                     /gene="ruvA"
                     /locus_tag="VYQ24_000073"
     CDS             7328..7921
                     /gene="ruvA"
                     /locus_tag="VYQ24_000073"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1967270.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASVENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7931..8494
                     /locus_tag="VYQ24_000074"
     CDS             7931..8494
                     /locus_tag="VYQ24_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1967271.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPVKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8491..9168
                     /locus_tag="VYQ24_000075"
                     /pseudo
     CDS             8491..9168
                     /locus_tag="VYQ24_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(9309..10340)
                     /locus_tag="VYQ24_000076"
     CDS             complement(9309..10340)
                     /locus_tag="VYQ24_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1967272.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEEPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDEIFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(10595..11278)
                     /locus_tag="VYQ24_000077"
     CDS             complement(10595..11278)
                     /locus_tag="VYQ24_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1967273.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPH
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11290..12234)
                     /locus_tag="VYQ24_000078"
     CDS             complement(11290..12234)
                     /locus_tag="VYQ24_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1967274.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12366..15197
                     /gene="uvrA"
                     /locus_tag="VYQ24_000079"
     CDS             12366..15197
                     /gene="uvrA"
                     /locus_tag="VYQ24_000079"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1967275.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKNVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMTAFGVDMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVVTNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15190..16251
                     /locus_tag="VYQ24_000080"
     CDS             15190..16251
                     /locus_tag="VYQ24_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1967276.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16383..16781
                     /gene="spx"
                     /locus_tag="VYQ24_000081"
     CDS             16383..16781
                     /gene="spx"
                     /locus_tag="VYQ24_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1967277.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16847..17416
                     /locus_tag="VYQ24_000082"
     CDS             16847..17416
                     /locus_tag="VYQ24_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1967278.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17503..17769
                     /locus_tag="VYQ24_000083"
     CDS             17503..17769
                     /locus_tag="VYQ24_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1967279.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17773..18192
                     /gene="ruvX"
                     /locus_tag="VYQ24_000084"
     CDS             17773..18192
                     /gene="ruvX"
                     /locus_tag="VYQ24_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1967280.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18208..18513
                     /locus_tag="VYQ24_000085"
     CDS             18208..18513
                     /locus_tag="VYQ24_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1967281.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            19124..20374
                     /locus_tag="VYQ24_000086"
     CDS             19124..20374
                     /locus_tag="VYQ24_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1967282.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISIFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20371..20901
                     /locus_tag="VYQ24_000087"
     CDS             20371..20901
                     /locus_tag="VYQ24_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1967283.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTG
                     QDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIV
                     VDDMDRDPSDQIVLTK"
     gene            21047..22579
                     /gene="cls"
                     /locus_tag="VYQ24_000088"
     CDS             21047..22579
                     /gene="cls"
                     /locus_tag="VYQ24_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1967284.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22657..22800
                     /locus_tag="VYQ24_000089"
     CDS             22657..22800
                     /locus_tag="VYQ24_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967285.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            22837..24375
                     /locus_tag="VYQ24_000090"
     CDS             22837..24375
                     /locus_tag="VYQ24_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1967286.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSLRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24495..26702
                     /gene="nrdD"
                     /locus_tag="VYQ24_000091"
     CDS             24495..26702
                     /gene="nrdD"
                     /locus_tag="VYQ24_000091"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1967287.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26714..26854
                     /locus_tag="VYQ24_000092"
     CDS             26714..26854
                     /locus_tag="VYQ24_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967288.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26898..27404
                     /locus_tag="VYQ24_000093"
     CDS             26898..27404
                     /locus_tag="VYQ24_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967289.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            27397..27987
                     /gene="nrdG"
                     /locus_tag="VYQ24_000094"
     CDS             27397..27987
                     /gene="nrdG"
                     /locus_tag="VYQ24_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1967290.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLEFLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27984..28601
                     /locus_tag="VYQ24_000095"
     CDS             27984..28601
                     /locus_tag="VYQ24_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1967291.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28867..29175
                     /gene="rpsJ"
                     /locus_tag="VYQ24_000096"
     CDS             28867..29175
                     /gene="rpsJ"
                     /locus_tag="VYQ24_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1967292.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29392..30018
                     /gene="rplC"
                     /locus_tag="VYQ24_000097"
     CDS             29392..30018
                     /gene="rplC"
                     /locus_tag="VYQ24_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1967293.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            30043..30666
                     /gene="rplD"
                     /locus_tag="VYQ24_000098"
     CDS             30043..30666
                     /gene="rplD"
                     /locus_tag="VYQ24_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1967294.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30666..30962
                     /locus_tag="VYQ24_000099"
     CDS             30666..30962
                     /locus_tag="VYQ24_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1967295.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30980..31813
                     /gene="rplB"
                     /locus_tag="VYQ24_000100"
     CDS             30980..31813
                     /gene="rplB"
                     /locus_tag="VYQ24_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1967296.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31917..32198
                     /gene="rpsS"
                     /locus_tag="VYQ24_000101"
     CDS             31917..32198
                     /gene="rpsS"
                     /locus_tag="VYQ24_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1967297.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            32210..32554
                     /gene="rplV"
                     /locus_tag="VYQ24_000102"
     CDS             32210..32554
                     /gene="rplV"
                     /locus_tag="VYQ24_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1967298.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32567..33220
                     /gene="rpsC"
                     /locus_tag="VYQ24_000103"
     CDS             32567..33220
                     /gene="rpsC"
                     /locus_tag="VYQ24_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1967299.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            33224..33637
                     /gene="rplP"
                     /locus_tag="VYQ24_000104"
     CDS             33224..33637
                     /gene="rplP"
                     /locus_tag="VYQ24_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1967300.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33647..33853
                     /locus_tag="VYQ24_000105"
     CDS             33647..33853
                     /locus_tag="VYQ24_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1967301.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33878..34138
                     /gene="rpsQ"
                     /locus_tag="VYQ24_000106"
     CDS             33878..34138
                     /gene="rpsQ"
                     /locus_tag="VYQ24_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1967302.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            34164..34532
                     /gene="rplN"
                     /locus_tag="VYQ24_000107"
     CDS             34164..34532
                     /gene="rplN"
                     /locus_tag="VYQ24_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1967303.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34610..34915
                     /gene="rplX"
                     /locus_tag="VYQ24_000108"
     CDS             34610..34915
                     /gene="rplX"
                     /locus_tag="VYQ24_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1967304.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34939..35481
                     /gene="rplE"
                     /locus_tag="VYQ24_000109"
     CDS             34939..35481
                     /gene="rplE"
                     /locus_tag="VYQ24_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1967305.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35499..35768
                     /gene="rpsN"
                     /locus_tag="VYQ24_000110"
     CDS             35499..35768
                     /gene="rpsN"
                     /locus_tag="VYQ24_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1967306.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            36053..36451
                     /gene="rpsH"
                     /locus_tag="VYQ24_000111"
     CDS             36053..36451
                     /gene="rpsH"
                     /locus_tag="VYQ24_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1967307.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36643..37179
                     /gene="rplF"
                     /locus_tag="VYQ24_000112"
     CDS             36643..37179
                     /gene="rplF"
                     /locus_tag="VYQ24_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1967308.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            37263..37619
                     /gene="rplR"
                     /locus_tag="VYQ24_000113"
     CDS             37263..37619
                     /gene="rplR"
                     /locus_tag="VYQ24_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1967309.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37637..38131
                     /gene="rpsE"
                     /locus_tag="VYQ24_000114"
     CDS             37637..38131
                     /gene="rpsE"
                     /locus_tag="VYQ24_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1967310.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            38103..38327
                     /gene="rpmD"
                     /locus_tag="VYQ24_000115"
     CDS             38103..38327
                     /gene="rpmD"
                     /locus_tag="VYQ24_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1967311.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38472..38912
                     /gene="rplO"
                     /locus_tag="VYQ24_000116"
     CDS             38472..38912
                     /gene="rplO"
                     /locus_tag="VYQ24_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1967312.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38925..40235
                     /gene="secY"
                     /locus_tag="VYQ24_000117"
     CDS             38925..40235
                     /gene="secY"
                     /locus_tag="VYQ24_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1967313.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40386..41024
                     /locus_tag="VYQ24_000118"
     CDS             40386..41024
                     /locus_tag="VYQ24_000118"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1967314.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            41141..41359
                     /gene="infA"
                     /locus_tag="VYQ24_000119"
     CDS             41141..41359
                     /gene="infA"
                     /locus_tag="VYQ24_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1967315.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41384..41500
                     /gene="rpmJ"
                     /locus_tag="VYQ24_000120"
     CDS             41384..41500
                     /gene="rpmJ"
                     /locus_tag="VYQ24_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1967316.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41518..41883
                     /gene="rpsM"
                     /locus_tag="VYQ24_000121"
     CDS             41518..41883
                     /gene="rpsM"
                     /locus_tag="VYQ24_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1967317.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41901..42284
                     /gene="rpsK"
                     /locus_tag="VYQ24_000122"
     CDS             41901..42284
                     /gene="rpsK"
                     /locus_tag="VYQ24_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1967318.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            42327..43262
                     /locus_tag="VYQ24_000123"
     CDS             42327..43262
                     /locus_tag="VYQ24_000123"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1967319.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            43274..43660
                     /gene="rplQ"
                     /locus_tag="VYQ24_000124"
     CDS             43274..43660
                     /gene="rplQ"
                     /locus_tag="VYQ24_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1967320.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43925..44191
                     /locus_tag="VYQ24_000125"
     CDS             43925..44191
                     /locus_tag="VYQ24_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1967321.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            44201..45538
                     /locus_tag="VYQ24_000126"
     CDS             44201..45538
                     /locus_tag="VYQ24_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1967322.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            45782..46474
                     /locus_tag="VYQ24_000127"
     CDS             45782..46474
                     /locus_tag="VYQ24_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1967323.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(46577..48212)
                     /locus_tag="VYQ24_000128"
                     /pseudo
     CDS             complement(46577..48212)
                     /locus_tag="VYQ24_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(48202..49293)
                     /locus_tag="VYQ24_000129"
     CDS             complement(48202..49293)
                     /locus_tag="VYQ24_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967324.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            complement(49306..49674)
                     /locus_tag="VYQ24_000130"
                     /pseudo
     CDS             complement(49306..49674)
                     /locus_tag="VYQ24_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT        14981 a         9368 c        10957 g        14416 t
ORIGIN      
        1 aaacattgtt agaaatcgat ttgactgtcc tgattgattt atcctgttct tatttcattt
       61 cactatagta aggtgcagca ggatacagca aaaccgagaa gtttctttct cggttttgct
      121 tattatgagg ttcctatttt ctatgctcca agagttgatt gatgtgatct acttttttag
      181 cctctctttt atagagaata gcccagatga tgagatagac aatcgcaaac tcgataatga
      241 gctggaggta gaaaatccag tggaagggga accaaccagc aagagttgct agtgggacaa
      301 agccagccag cataaggaag aaatgagtca gagtggcacg aagcatgctc cagtcacggc
      361 tgaataagcg gttgccaaag ttgaagagca taccgatggc tgcccagata agtgtgcagt
      421 agagcaagac cagggcaccg tgaacctgat gctgatccat cacttggccg atgagagagt
      481 agggatttag tggtgcgtag gtatttggtg cataaatgag tgaaaagagg atggagagga
      541 tgaggccgat aagaacaccg gcagctgcat cgtgaaatac ttgttttttc atagttctaa
      601 tttctccttg atggttttta gataacggcg tgaagagtag gtgaagcttt cgtttttcaa
      661 gaaaatttct accagaccgt ttggcgtgag cttgaggtga gagatggaat cgatattgat
      721 gatttctgat tgggaaattt ggataaaatt gcttggcaag agtttaagaa cctgatagag
      781 tcgcaaatca atgctgtagg tctgactcgc ggtttctgct agaaccttcc gattctcgat
      841 atagaagcgc tgaatcttgc caatctcaac tagatagacc tgatcatcga tttttccttt
      901 gattttttct ctttggtcca gattttctgc gaactcgatg actttctgga ctttttcggt
      961 ttcttgaggt gcttggacaa tcagcttttc ctcctcgtaa gtctcactaa tctgtagttc
     1021 tactttcata gttttctctc cttttcagtt atacaaggtt gtgatcactt cctgtatatc
     1081 cttattaaac actattttat agcccatggc aaggaacctt gtctatgtgg agggattggg
     1141 ctcctatgtg gtggagcttt tctgttcttt ctgaaatatg gtataatagc actaattaat
     1201 ttctaggaaa atagatacag aaaggggctg aaagatgtct catattattg aattgccaga
     1261 gatgctggca aaccaaatcg cagctggaga ggtcattgaa cgtcctgcca gtgtggtcaa
     1321 agagttggta gaaaatgcca ttgacgcggg ctctagtcag attatcattg agattgagga
     1381 agctggtctc aagaaggttc aaatcacgga taacggtcat ggaattgccc acgatgaggt
     1441 ggagttggcc ctgcgtcgcc atgcgaccag taagataaaa aatcaagcag atctctttcg
     1501 gattcggacg cttggttttc gtggtgaagc cttgccttct attgcgtctg ttagtgtctt
     1561 gactctgtta acggcggtgg atggtgctag tcatggaacc aagttagtcg cgcgtggggg
     1621 tgaagttgag gaagtcatcc cagcgactag tcctgtggga accaaggttt gtgtggagga
     1681 tctctttttc aacacgcctg cccgtctcaa gtatatgaag agccagcaag cggagttgtc
     1741 tcatatcatt gatattgtca accgtctggg cttggcccat cctgagattt cttttagctt
     1801 gattagtgat ggcaaggaaa tgacgcggac agcagggact ggtcaactgc gccaagcaat
     1861 cgcagggatt tacggtttgg tcagtgccaa gaagatgatt gaaattgaga actctgacct
     1921 agatttcgaa atttcaggtt ttgtgtcttt acctgagttg actcgggcta accgcaatta
     1981 tatcagcctc ttcatcaatg gccgttatat caagaacttc ctgctaaatc gtgctatttt
     2041 ggatggtttc ggaagcaagc ttatggttgg acgttttcca ctggctgtca ttcacatcca
     2101 tatcgaccct tatctagcgg atgtcaatgt gcatccaact aagcaagagg tgcggatttc
     2161 caaggaaaaa gaactgatga ccctggtttc agaagctatt gcaaatagtc tcaaggaaca
     2221 aaccttgatt ccagatgcct tggaaaatct tgccaaatcg accgtgcgca atcgtgagaa
     2281 ggtggagcaa actattctcc cactcaaaga aaatacgctc tactatgaga aaactgagcc
     2341 gtcaagacct agtcaaactg aagtagctga ttatcaggta gaattgactg atgaagggca
     2401 ggatttgacc ctgtttgcca aggaaacctt ggaccgattg accaagccag caaaactgca
     2461 ttttgcagag agaaagcctg ctaactacga ccagctagac catccagagt tagatcttgc
     2521 tagcatcgat aaggcttatg acaaactgga gcgagaagaa gcatccagct tcccagagtt
     2581 ggagtttttc ggacaaatgc acgggactta tctctttgcc caagggcgag atggacttta
     2641 catcatagat cagcacgctg ctcaggaacg ggtcaagtac gaggagtacc gtgaaagcat
     2701 tggcaatgtt gaccagagcc agcagcaact cctagtgccc tatatctttg aatttcctgc
     2761 ggatgatgcc ctgcgtctca aggaaagaat gcctctctta gaggaagtgg gcgtctttct
     2821 agcagagtac ggagaaaatc aatttattct acgtgaacat cctatttgga tggcagaaga
     2881 agagattgaa tcaggcatct atgagatgtg cgacatgctc cttttgacca aggaagtttc
     2941 tatcaagaaa taccgagcag agctggctat catgatgtct tgcaagcgat ctatcaaggc
     3001 caatcatcgt attgatgatc attcagctag acaactcctc tatcagcttt ctcaatgtga
     3061 caatccctat aactgtcctc acggacgtcc tgttttggtg cattttacca agtcggatat
     3121 ggaaaagatg ttccgacgta ttcaggaaaa tcacaccagt ctccgtgagt tggggaaata
     3181 ttaaaagtat aaaaaagtct gggaaaaatt ttcaaaatca aaaaaacgca taaaatcagg
     3241 tgttcaaaaa ccttgatttt atgcgtttta tcatggaaat agttacttca ttttttccta
     3301 attcttttcg aaactctttt taaacgacgt cagttttatc agtaatctca aaacagtgtt
     3361 ttgagctaat tttgccagtt ttgtctgtaa catcgaagtt gtgttttacc actctgcgac
     3421 tggtttccta gtttgctcta tgattttcac agagtattaa attgcgattt tgccaagttt
     3481 ctttattcgt ctaaaagtag agtctgttct atgcgtctaa tgtacgaatt aggttgacca
     3541 tttcaatagc tccttgtgca cactcagaac ccttatttcc tgctttagta ccagctcgtt
     3601 ctatggcttg ttcaattgta tctgtcgtta gcacaccaaa cataacagga atttcgctat
     3661 ttaaactgat ttgggcgatt cccttagata cctcgctaca tacataatca taatgacttg
     3721 tatttcctct aatgacagct cccaagcaga taattgcatc atatttttta ctttttgcca
     3781 tttttgatgc aatcagtgat atttcaaaag ctcctggaac ccaggctacc tcgatatctt
     3841 tctcgtttac attctctctt ttgagattat ctagtgctcc agataataat tttgaagtta
     3901 taaattcatt aaatctcgct acaacaatac ctattttaat attgtttgct actaaattac
     3961 cttcataagt gttcatttat ttttcctcca tatttaaaat gtgacccatt cgatttttct
     4021 ttgtttctaa ataaaaacta tcgtaaggat tggcttctat ttcgattgat attctactgg
     4081 aaatggtaat tccatatttt tctaactgtt caaccttgtc aggattattt gtcagtaaat
     4141 gaagtgactg aagtcccaga tctttaagca tttgtgctcc aatatgatat tctcttaaat
     4201 caccttcaaa gcctaatgca agattggcat caagcgtatc catgccttga tcttgtaaat
     4261 gataggcttt taatttattg ataagtccaa ttcctcgtcc ctcctgtcgc aagtaaagta
     4321 agacacccga accattctca acaatcattt tcatagcttt atcgaattgc tgtccacaat
     4381 cgcaacgtaa ggagcctaaa acatcccctg ttaaacattc ggagtggacc cgacataata
     4441 cattggcttc atcctctatg tttcccataa taagagcaag atgatgttcc ccatttagtt
     4501 tatctatata gctaattgct ttgaaattac cgtatctagt aggcatattg acagttgaaa
     4561 ctcgttctac cagctgatca tatacttttc tatattcttg taattctttg atggtaatta
     4621 gtggaatgtt gtgtttcttc gagaactgaa ttaaatcatc tgttctcatc attttgccat
     4681 catgattcat tatttcacaa cataggccac actcttttag tccagctaat tttaataaat
     4741 caacagttgc ttctgtgtgt ccatttcttt ctagaacacc accttttttt gcaattaaag
     4801 gaaacatgtg tcctggcctg cgaaaatcag agggtgttat atcttcagct acacacatac
     4861 gtgcggtcag tcctctttcc tcggcagaaa tacctgtggt cgtttcttta taatcaattg
     4921 aaactgtaaa agcagtctta tgattatctg tattgttttc aaccataggt gaaagcatta
     4981 attgattagc taaactttcg ctcataggca tacaaattaa tcctttggca taagtagcca
     5041 taaaattaac attttctgtt gtagctgctt gtgcagaaca aattaagtct ccttcatttt
     5101 ctctatcctt gtcgtctata acaagaacaa gtcgtccctt ctgcaatgct tctaatgctt
     5161 cttgtatttt tcgatattcc attgactgat tatcctttct gctaaaatcc attttgatat
     5221 aatagttcct tagatatttc tgattttgga gagttatcca tcagtttttg cacatattta
     5281 cctaagatat cattttcaag atttactgta ctcccgactt gtttactctt aagaatggtt
     5341 tgttccaagg tatgagggat aacagatact gaaaagttta ctttggagac tttagcgaca
     5401 gtcagactaa tgccgtcaat tgtaatagat cctttttcaa ctattaaatc taaaatttct
     5461 ttttgtgtgt tgatttgata ccatacagca ttatcatctt tttttattga cgagattttt
     5521 cctgtaccat caatgtgtcc tgtaacgacg tgacccccaa gtcgaccgtt gacagataag
     5581 gctctttcta gattcacctc acttccatgt tttaatagag taagagctgt tcgactccat
     5641 gtttcattca ttacatcaac tgtaaaggat tgatgattga aatgagtaac tgtaagacag
     5701 ataccattta ctgctatact atcgcctaaa tggatatccg ttaatatttt tgaggcttta
     5761 attgatagtt tacaattacg agagtctttc tgtattcttt caacttttcc gatttcttca
     5821 attattcctg tgaacatgga taaatcactt cactttctat gagatagtca tttcctattt
     5881 gagaaaatgc ataaggtttc aatctaatag cgccatttgg caaagaaata ccttcacctc
     5941 cgacaggaaa cttggcacta cctccaaaaa tttttggtgc aatatatatt ttcagctcat
     6001 caacaatttg ttgttccaaa gcactccaat tcattagact gcccccttct agaactaggc
     6061 tatcaatctg catgtttcct agatgttgca ttaaactcga taagtctata tgattgcctt
     6121 ttttctttat ggaaagtatt tcacagccat gattttgata tagcttcatt ttatttttgt
     6181 cttcagagga agtggcaatg taagttttaa tatcatttgc tgtttttacg attttagagg
     6241 taagaggagt tcgtaaatgt gtatcgcata tgatacggat aggatttttc ccttcctcca
     6301 atctacatgt cagcaaagga tcgtcttgaa taacagtatt gactcccacc ataattgcac
     6361 taacatagtg tcgtaactga tgcacatgct ttcttgcttc ttcttcagta atccatttgg
     6421 attgatttgt tttagtggct atttttccat ccattgacat tgcatatttc ataaaaacat
     6481 agggtacatg ctgggtaata tactttctaa aactttttat taagttaaga cactcatttt
     6541 ctaaaattcc aacagtaact tgaagattat tttcctcaag tatctttact ccttttccag
     6601 atacaatagg attacagtct aggcttccaa tgactactct tgtaatacca ctatcgatta
     6661 tagcatctat acagggaggt gttttcccga agtgacaaca gggttcaagt gttacataaa
     6721 gcgtcgctcc gacaggggat tttctacagt ttttaagagc atttctctca gcatgtgggc
     6781 caccaaaaaa ctcatgataa ccttgtccga tgatgtgatt atcttttaca ataactgcgc
     6841 cgaccatagg attgggattg acgtaaccag cccctttttg tgccagtttt attgctaatt
     6901 tcatatattt tgaatcgctc atctcactac ctccaaaaaa aatatacctt gaatagggga
     6961 ctactcaagg catacaaaag aaaacttata cgattaacaa aaatactctg aaatgacaag
     7021 taatcatttc agagcacgca aaaagcacaa atatactttt atcttctttc atccagacta
     7081 tactgtcggc tttggaattt caccaaatca tgcctttcgg ctcgtgggct ataccaccgg
     7141 tagggaattg caccctgccc tgaagatagt tattcaatta cagatgatta tagtacttaa
     7201 ttttgaatat gtcaacagat aaataccgat tgtttttgat atactgtatt tgtgataatc
     7261 gattctcgct cctcggataa agaaaatatg atatactaga taaacgaaat aagagagaag
     7321 gaatactatg tacgcatatt taaaaggaat cattaccaaa attactgcca aatacattgt
     7381 tcttgaaacc aatggtattg gttatatcct gcatgtggcc aatccttatg cctattcagg
     7441 tcaggttaat caggaggctc agatttatgt gcatcaggtt gtgcgtgagg acgcccattt
     7501 gctttatgga tttcgctcag aggatgagaa aaagctcttt cttagtctga tttcggtctc
     7561 tgggattggt cctgtatcag ctcttgctat tatcgctgct gatgacaatg ctggcttggt
     7621 tcaagccatt gaaaccaaga acatcaccta cttgaccaag ttccctaaaa ttggcaagaa
     7681 aacagcccag cagatggtgc tggacttgga aggcaaggta gtagttgcag gagatggcct
     7741 tcctgccaag gtcgcagtgc aagcaagtgt tgaaaaccaa gaattggaag aagctatgga
     7801 agccatgttg gctctgggct acaaggcaac agagctcaag aaaatcaaga aattctttga
     7861 aggaacgaca gatacagctg agaactatat caagtcggcc cttaaaatgt tggtcaaata
     7921 ggagcagaga atgacaaaac gttgttcgtg ggtcaagatg accaacccac tctacatcgc
     7981 ctatcatgat gaggagtggg gccagcccct ccatgatgac caagcgttgt ttgagttgtt
     8041 gtgtatggaa acctatcagg caggcctgtc ttgggaaacg gtactcaaca aacgccaagc
     8101 tttccgagaa gcctttcata gctatcaaat tcactcagtc gcagagatga ctgacactga
     8161 attggaagcc atgctggaga atccagctat cattcgaaat agagccaaga tttttgctac
     8221 acgcgctaac gcccaagctt ttctacagtt acaggcagag tacggctctt ttgatgccta
     8281 tctttggtct tttgttgagg ggaaaactgt cgttaacgat gttcctgatt atcgccaagc
     8341 cccagttaaa acacccttat ctgagaaatt agccaaagat ctcaaaaaac gaggcttcaa
     8401 gttcacaggc ccagtcgccg tattgtcttt tctacaggct gcagggctag ttgatgacca
     8461 cgagaatgat tgtgagtgga aaggtcttaa atgatgtcta acaaaaataa ggaaattctg
     8521 atttttgcga ttctctatac agtcctcttt gtgtttgatg gcgttaaatt gctggcttct
     8581 ttaatgccat ctaccattgc aaattatctt gtttatgtag ttttagcttt atatggctcc
     8641 ttcttgttca aggatagatt gatccaataa tggaaggaga ttagaaagac taaaagaaaa
     8701 ttcttctttg gagtcttaac aggatggctc tttctcattc tgatgactgt tgtctttgaa
     8761 tttgtatcag agatgttgaa gcagtttgtg ggactagatg gacaaggtct aaatcagtct
     8821 aatattcaaa gtacctttca agaacaacca ctactgatag ctgtttttgc ttgtgtcatt
     8881 ggacctctga tagaagaatt atttttccgt caggtcttat tgcattactt gcaggaacgg
     8941 ttgccaggtt tactaagcat tattctggta ggacttgttt ttgctctgac tcatatgcac
     9001 agtttggctc tatcagagtg gattggtgca gttggttact taggtggagg ccttgccttt
     9061 tctattattt atgtgaaaga aaaagaaaat atctactatc ccctacttgt tcacatgtta
     9121 agcaacagcc tctccttaat cattttagtt atcagtatag taaaatgaaa taagaacagg
     9181 acagtcaaat cgatttctaa caatgcttta gaagtagagg tgtactattc tagtttcaat
     9241 acactgtaac atgtgatgaa aatgccagta atgataccga gaaaaaagct gagaaacttt
     9301 tcccagcttt atttgttata gtcaaagcga atgacttgtt cctgtgcatc tacatgagca
     9361 tggaccccaa agggtacaat tgctcttgga gttgcgtggc cgacattcag attatagaca
     9421 atcgggatat tgttgtcaat gatatccaat agtgcctctt tatagtcgtc atggaaaatt
     9481 tcatccatag gttttccgac caagagtcca ttgatgaccg cgaatatgcc agtgtccttt
     9541 aaagttagca acatcttttt gaagtcttct ggcttaggct cttcttcgct tgtttcgagc
     9601 aagaggattt tcccttccca gtctgacaag tcagggaaaa gtttgtattt ttggcagagt
     9661 tccgtgctat ctgcgtatcg agagttgtca aagatatcgt agagggattc gaggcaacca
     9721 ccgaggattt tcccctcgaa ctgggcactt ccttgcaaca agtcaaaacc tgtatttgta
     9781 tgactgacac gaggtgttcc cagggccgtg ggactaaaat cagttcgttc ctcataccaa
     9841 acgtcactag ggcggatttc tgaaattctt cccgtctcaa tcaattcttt aaagtagtga
     9901 aggctatagg ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa ggattgacca
     9961 taaaaagtct tgattcctaa tttatgcaac atgaggtggt tcatggttgt atccgagaag
    10021 ccaagaaaaa ttttttgttt gataaccttt tggagttggt cattttcaaa aagataaggt
    10081 agcaagcgat aggtatcgtc tccaccgatg gcacatagga tcatgtcgat gctatcatca
    10141 gaaaaggcat gaatcaaatc ctctgcacga gcttcaggat ggtccttgat aaagtctaat
    10201 ccttttaacg aatggggcaa aaagatggga ttgagtccca gatccttgag acgttggata
    10261 cccaagtcca cttcgtgttt gacaaaatcc tctccgataa tgccactaga taaactaaca
    10321 ataccaatag tagaaaccat atctcatcct cctagaaata gattgagcct attttatcac
    10381 aaaatataag agaaagctat gtgggatttc agaagaatac ttttaatact cttcgaaaat
    10441 ctcttcaaac cacgtcagtt ttatctgcaa cctcaaagct gtgctttgag caacctgcag
    10501 ctagcttcct agtttgctct ttgattttca ttgagtataa ctcaaaaaag taagtgaaaa
    10561 agcaaccgca cctgcagttg cttttatcgt tttcttattc ttgatagcgt gttggtcctg
    10621 cacttgcact acggatattc aagcgtttct tgagatagaa gcgaagggct aggagggctg
    10681 ctccaataat agctagtggc aatggatcca gtactgggtt aaggctagct ggtaggaagc
    10741 ttgttgcaaa gaagacaagc aaccaaagga acatagaagc taggataact agtacagatt
    10801 tccagaaagg tggacgttga ctgcgatcca tatctggtcc atagtattgg taaacaaagt
    10861 agtacatcaa gtagaaggca aatccaccaa ccagtccaac taatagaaga gtaatcaatc
    10921 catagccgaa agcttgatct gccgcaaaga aggttgtgag ggcgctgaca agggcaaaga
    10981 ggctagtgat gaaaagagct gagtccataa tcatcagttt tggatcatca ttttcttttg
    11041 gatgctcttt ttcgtactgc tctttgacag tgaagctatg agcccaatga gttggtgcgc
    11101 cgtataggga acgggcagtt gtacctttag attgctcctc aaggatgtgg ggaatgactt
    11161 cctcaaaaat agtctggatt tcagcgtctg ttttcccatc tttgatgaat tgttgggtag
    11221 caatgtggac aaactcttgg tttttcttag ttaatttttg tagatcaatt tgagacatag
    11281 gaactcctct tagaaccatt ttttatggat gagatagaga gtgagcgaga cactcatagc
    11341 aaaggcgata aagacgatta accagaaggc atttggctct ccgtttaggg ggatttcatt
    11401 atccttaaag ttcatcccgt aggcagaaaa gaccatggtt gggatggaca tgacgatggt
    11461 cacaagggcc aaggttttca tgatgttgtt ctggttgtta gaaatgatag aggcaaaggt
    11521 ctctgtcata gaatgcaaga cgtttccata aatatctgcc atctcgatgg cctgttgggt
    11581 ttcaatcagg gtgtcttcaa gcaggtcttc gtcctcaagg tatttcttga tattgctggt
    11641 tgaactggtc aatttcttaa tcacgcgctc atttgttttg agggaggcct tgaaatagac
    11701 gatagttttt tccaattcca tgagctcaat caattcttca ttacgagttg attgatgcag
    11761 ttgactttcg atttgttcac tcttgcggtc gattgaacga agggctgtta ggtaaagctc
    11821 tgcattgcga taaagaattt gaaagataaa acgtgaacgc atgaaggtat agaaattacg
    11881 caatcgacgg ttgataaaga catcaaggac aggtagtggt tccaaacacg tagtgataat
    11941 ggtttcctca gtgatgataa taccaagcgg gatggttacg tagtaggtgc ggttatttct
    12001 ttcctccgtg accggcacgt ctacgataat cagggtatac tcgtcttcaa tggtaatacg
    12061 agacatttct tccgcatcga gcggtgctcg aaggtcggca atatcaatat cgaaggtgtt
    12121 ggcgatttcg agtgattcat tttgagtcgg attgacgaga ttgatccaag tacccggttc
    12181 aagcgtatcg atctctttaa attcagttgt tgtagagaga aaaacttgtt tcatatccct
    12241 tatcctttct catttttcag attttttcac actgtactat tatactacaa aatcggcctt
    12301 ttgggtaata gaatctcact ttttttggta taatggtaag caataatgga ctagaaagaa
    12361 caaagatgca agataaaatt gtcattcatg gggcgcgtgc ccataattta aaaaatattg
    12421 atgtggagat tccgcgagac aagttggttg tcgtgactgg cttgtcaggt tcagggaagt
    12481 ccagtctggc ttttgatacc ctctatgcgg agggacaacg tcgctatgta gagagtttgt
    12541 ccgcctacgc tcgtcagttc ttgggaaata tggaaaaacc agatgtagat gccattgatg
    12601 gtctcagccc agctatttcc atcgaccaga aaacgactag taaaaatcct cgctcgacgg
    12661 tgggaacgac gactgaaatc aatgactatc tgcgtctcct ctacgcacgt gtggggacgc
    12721 cttactgtat caacggacat ggagctatca atgcttcttc tgtggagcaa atcgttgata
    12781 aggttttgga gttacctgaa cgccagcgct tgcagatctt ggctcctgtc atccgcaaga
    12841 aaaaaggcca acataagaat gttatcgaga aggttcagaa agacggatat gttcgtgtcc
    12901 gtgtggatgg ggaagtctat gatgtgaccg aagtgccaga gttgtctaag agcaagcaac
    12961 acaatatcga tgtcgtggtt gaccgtattg ttatcaagga gggcattcgt agccgtctct
    13021 ttgattccat tgaagctgcc cttcgtatcg cagaaggtta tgtgattatc gacaccatgg
    13081 acgattcgga gttgctcttc tctgagcatt atgcctgtcc agtttgtgga tttactgtcc
    13141 cagagttaga gcctcgtctc ttctctttca atgctccctt tggctcttgt agtgagtgtg
    13201 acggcttggg gattaagctg gaggtggata ctgatttggt agtgccagat gccagcaaaa
    13261 cgctacgtga gggggctttg gcaccttgga atcctatctc atctaactac tatccaaata
    13321 tgctagagca ggctatgaca gcctttggag tggatatgga taagcccttt gaggacttgt
    13381 cagaagaaga taagaacttg attctctacg gctcggatgg caaagaattt cattttcact
    13441 atgagaatga atttggcggt gtgcgcgata ttgacattcc ttttgaggga gtcgtcacta
    13501 atatcaagcg tcgttaccat gagactaaca gtgattacac gcgcactcag atgcgtctct
    13561 acatgaatga gctgacctgc ggaacctgtc aaggctatcg tctcaatgac caggccttgt
    13621 ctgtccgtgt gggcggccag caagggccac atattggaga aatctcagac ctgtctatcg
    13681 ctgaccactt ggacttggtg agccagttaa ctttgtctga aaatgaagcc atcattgctc
    13741 gtcccattct caaggaaatc aaggatcgtt tgaccttcct taataatgtg ggtcttaact
    13801 atctaaccct gtcacgttca gcaggaaccc tttcaggtgg ggaaagtcag cgcattcgtt
    13861 tggcaaccca gattggttcc aacctatcag gtgtcctcta tatcctagac gagccgtcaa
    13921 tcggtcttca ccagagggac aatgatcgcc tgattgccag tctgaaaaag atgcgtgact
    13981 tgggcaatac tcttatcgtg gtggaacacg acgaagatac catgcgtgag gctgattatc
    14041 tgattgacgt tggtcctggt gccggtgttt ttggtggtga gattgttgca gcaggtacac
    14101 ctaagcaagt ggctcgcaac agcaagtcta tcacaggtca gtacttgtca ggcaaacgtg
    14161 tcatcccagt accagaagag cgccgtgtcg gaaatggtcg ttttattgaa gtgataggag
    14221 cgcgtgagaa caacttgcaa aatgtcacgg ctcgctttcc actaggaaaa tttatcgcag
    14281 tgacaggtgt gtcgggttca gggaaatcga ctctaatcaa cagcattctc aaaaaagcca
    14341 ttgcccagaa gctcaaccgt aattcagaca aacctggtaa gtttaagact attacaggga
    14401 ttgagcatgt cgaccgtttg attgatattg accagagccc tatcggacgg acgccgaggt
    14461 ctaacccagc tacctataca ggagtttttg acgatatacg tgaccttttt gctcagacaa
    14521 atgaagccaa gattcgaggc tacaagaagg ggcgcttcag tttcaacgtc aagggaggtc
    14581 gctgtgaagc ttgctcaggt gacgggatta tcaagattga gatgcacttc ttgccagatg
    14641 tttatgtggc ttgtgaagtt tgccacggaa cccgctataa cagtgaaacc ctagaagttc
    14701 actacaagga aaagaatatt tcgcaggtct tggatatgac ggtcaacgat gcggtagaat
    14761 ttttccaaca cattccgaaa attcaacgca aactccagac catcaaggat gtagggctag
    14821 gctatgtgac cttgggacag ccagctacca ctctttctgg gggagaagcc caacgtatga
    14881 agttagctag cgaactccac aaacgctcga caggaaaatc tttctacatt ctggatgagc
    14941 cgacgacagg gcttcataca gaggacattg ctcgcctgct caaggtttta gctcgctttg
    15001 tagacgatgg caatacagtc ctcgtcatcg agcacaatct ggatgttatc aagacggcag
    15061 accatatcat tgacttggga cctgagggcg gtgtcggtgg tggaaccatc atcgtaacag
    15121 gaactccaga agaagtagct gccaacgaag ccagctatac aggacactat ttgaaaggaa
    15181 agttacatca tgaataaacg cgtacaagca tttctagcta aaatgcaaga aaaagaacta
    15241 gatggtatca tcatcaacaa tcttaaaaac gtctattatt tgactggttt ttggggctca
    15301 aacggaacag tctttatcag ccgtgaccgt caggtcttag tgacagactc tcgctatatc
    15361 atcgcagcta agcaagaaac cagtggtttt gagattgtgg ctgatcgtga tgaattggct
    15421 gtcattgcag gaattgttaa ggacatgggc ttgactcgta tcggttttga agatgagatt
    15481 tcagtgtctt attaccaccg tatgcaggca gcttttgcag gtttgaactt gcttccacaa
    15541 actcagtttg tggaaggtct tcgtatgatt aaggatgagg cagagattgc agcgattcgc
    15601 aaggcttgtt ctatctcaga ccaagctttc cgcgatgcgc ttgactttat caaaccagga
    15661 aaaactgaaa ttgagattgc caacttcctt gatttccgca tgcgtgagtt gggagcatct
    15721 ggcttatctt ttgatacgat cctagctagc ggtatcaatt cttctaaacc ccatgcccat
    15781 ccaatgcaca aaccagtgga gttgggagaa gccattacca tggacttcgg ctgtctctat
    15841 gaccactatg tcagtgatat gacccggact atctatctag ggcatgttag cgatgagcag
    15901 gcagagattt acaatacggt tctaaaagct aaccaagcct tgattgacca agctaaggca
    15961 ggcttaggtt tccgtgactt tgacaaaatc cctcgtgata ttatcattga ggcaggttat
    16021 ggtgactact ttactcacgg cattggccac ggtattggtc tggatatcca tgaggaaccc
    16081 tactttagtc agacttctac agaaactatt aagacaggta tggccttgac cgatgaacca
    16141 ggtatctata tcgaaggcaa atatggcgtt cgtatcgagg atgatatcct gattacagag
    16201 acaggttgtg aattattgac cctagctcca aaagagttga tagtcattta gttgtttgtc
    16261 aagaaaaaag tacgaaaatg acgaaaaaag tcaaaaaaaa ttaaaaatag gtcgcaagtc
    16321 ggatgttttt tatggtataa tagactaaac tgatagtaac atgtagcgaa aggggtaggt
    16381 acatgattaa aatttataca gtctcaagtt gtactagctg taaaaaagca aaaacctggc
    16441 tcaatgccca ccagttaagt tataaagaac aaaaccttgg taaagaagga attacgagag
    16501 aagaattact ggatattcta accaaaacag ataacggaat agccagcatc gtttcgtcta
    16561 aaaatcgcta tgccaaagcc cttggagtgg atattgaaga tttgagtgtc aatgaagttc
    16621 tcaatctgat tatggaaaca ccgagaattt taaagagccc aatccttgta gatgaaaaac
    16681 gcctgcaagt tggctacaag gaagacgata ttcgtgcctt cctaccacgc tctgtccgta
    16741 atgtagaaaa tgcagaagca cgtttgcgtg cagctctata aacatcaagg ctgggagcac
    16801 ttcccagtct tattctattt taatttcaaa aagaaagaag aaagaaatga aaaaaatagt
    16861 tcttgttagt ctagctttcc tttttgtcct ggttggttgc ggacagaaaa aagaaactgg
    16921 accagctaca aaaacagaaa aagatacgct tcagtcggca ttgccagtta ttgaaaatgc
    16981 tgagaagaat acagttgtaa ccaagacttt ggtcttgccc aagtcagatg atggtagcca
    17041 gcagacacaa acaattactt acaaagacaa gacttttttg agtctaacta tccaacaaaa
    17101 acgtccagtc tctgatgagt tgaagactta tattgaccaa catggagtgg aggaaactca
    17161 aaaagctctt cttgaagcgg aggagaagga taagtctatc attgaagctc gtaaattggc
    17221 aggtttcaaa cttgaaacaa aactattgag cgcaacggaa cttcaaacaa cgactagttt
    17281 tgattttcaa gttctggatg tcaagaaggc ttcccagttg gaacatctga agaatattgg
    17341 tttggaaaat cttttgaaaa atgaaccaag caaatatatt tcagatagat tggcaaatgg
    17401 cgcgacagaa caatagaaaa tccaaataaa tagactgcct gaattgtaga aggtaaggaa
    17461 ttatgctata atggtactat tctaaggaaa gagggtgtta gaatgggatt tactgaagaa
    17521 acagtacgtt ttaaattgga cgattccaat aaaaaagaaa ttagcgaaac tttgacagat
    17581 gtttatgctt cgttgaacga taagggttac aacccaatta accaaatcgt aggttacgta
    17641 ttgagtggag accctgccta cgttcctcgt tataataatg cacgaaatca aatccgtaag
    17701 tatgagcgtg atgaaatcgt tgaggaattg gttcgctact acctcaaagg acaaggagtc
    17761 gatctataac ctatgagaat tatgggattg gacgtcggtt caaaaacggt aggggtggcg
    17821 attagcgatc cgcttggttt tacagctcaa gggcttgaaa tcatccagat aaatgaagaa
    17881 caaggccaat ttggttttga ccgcgttaag gaattggttg atacttacaa ggtggaacga
    17941 tttgtagtgg gcttgcctaa aaacatgaac aatacaagtg gaccgcgcgt agaagctagt
    18001 caagcctacg gagcaaagct agaagagttt tttggtttac cagtagacta tcaggatgaa
    18061 cgcttgacaa cagtggctgc tgagcgcatg ttgattgaac aagcagatat cagtcgcaat
    18121 aagcgcaaga aagtcattga taagttagca gctcagctga ttttacaaaa ttatttagat
    18181 agaaaatttt aatataaagg agaggctatg tcacacgatc ataaccacga ccacgaagaa
    18241 cgtgaactaa tcacactagt agatgagcaa ggaaatgaaa ccttgtttga aatccttttg
    18301 acaattgatg gaaaagaaga atttggtaaa aactatgttc ttctagtacc agttaacgca
    18361 gaagaagacg aagacggaca agttgaaatc caagcttact cattcatcga aaacgaagat
    18421 ggaacagaag gcgaattgca accaatccca gaagactcag aagacgaatg gaacatgatt
    18481 gaagaagtct tcaatagttt tatggaggag taaaaatgtt cagtgaatat ttttacccca
    18541 gttcctttaa ataagagaga actggtaagt ccgggggacg tttttagccc agctccttga
    18601 agtaagagag agctggtaag tccggggacg tttttagccc tgcttttaga aaaaagaaag
    18661 agcaggcagt ctgggagact gtatcagccc aactcccaga aatggggaag agttggaaca
    18721 tccagtggat gtttttagcc cagttccttt aaataagaga gaactggtaa gtccggggga
    18781 cgtttttagc ctgacgctaa aaataaaaac agtcagtaag ttcgggggat gttttagccc
    18841 agctccttga aataagatag agctggtaag tctgagaaac tgtatcagcc caactcccag
    18901 aaatggggaa gagttggaac gtccagtgaa catttttacc ccaactctta gaaattgaga
    18961 agagttggaa catctagtgg atgtttttag cccatattca cattcatagt agctagttat
    19021 tagctaacca ggtaagaggt cgggacgaaa atcctggcct tgtttttatt agtaaccata
    19081 ctttaatgcg ataattgatt ggacttttgt caaggagatg tgtatgtttg aagtagaaga
    19141 atggctccat agtcggattg gtttgaattt tcgatcaggt ttgggccgaa tacagcaagc
    19201 ggtggatttg ttaggaaatc ctgagcagtc ttaccctatt atccacgtaa cagggactaa
    19261 tggaaaagga tctaccattg cttttatgag ggaattattt atggggcatg gcaaaaaagt
    19321 tgcgaccttt acctcccctc atatcgtctc tatcaatgac cgaatctgca ttaatgggca
    19381 acctatagca gacgcagact ttatccgttt ggctgatcag gtcaaggaga tggagaaaac
    19441 gcttctgcaa actcctgacc agttgtcctt ttttgaattg ctgaccttag ttgcttttct
    19501 ttattttagg gagcaagagg tggatttggt tttattagaa gtgggaattg gtggcttact
    19561 tgacacgacc aatgtggtaa ctggagagct tgctgtcatc acctccattg ggcttgacca
    19621 tcaagaaacc ttgggtgata gtctagaagc aattgcagag cagaaagctg gtattttcaa
    19681 ggctggtaag aaggcagtga ttgcgaaatt gcctccagaa gctaggcttg tctgtcagaa
    19741 aaaagccgaa tctttagctg ttaaccttta tcaggcaggt caagattttt taatgctgaa
    19801 tggtgatttt tcaagctctt tactaaatct ttcacagctg aacataggct tagaaggagt
    19861 ctatcagcag gagaatgcag ccttggcgtt gcaaactttt cttcttttta tgggagaagg
    19921 aaaggaagct gttgatgaac aggctgtaag aaaggccttg gaacagaccc attgggctgg
    19981 tcgcttggag cgtattcgcc cacagattta cttggatggt gctcataacc tccctgcctt
    20041 gactcgcttg gttgagttta tcaaagaaaa agagcaggaa ggttatcgac ctcaaatcct
    20101 ctttggatcc ttgaaacgta aggattatca agggatgttg ggttatctga ctgaaaattt
    20161 acctcaagtg gaactcaaaa tgactggctt tgactatcaa gggtctttgg acgagacaga
    20221 tgtaacaggt taccatgtaa ttccctctta ccgagaattt atcagcattt tcgaagaaag
    20281 ggcagacgct caagacttgc tgttcgttac agggtctctc tattttatct cagaagtacg
    20341 gggctacctg ctggaccgtg agcagataaa ttgaccccct tgtttgaact tgttatacta
    20401 ggggaattgc tggttagaga aaatttctct aaattggtag cagaaaggaa attcatcatg
    20461 aaattaaaaa gattcacact ttctcttgct tctctagcaa gttttagtct cttagtagct
    20521 tgttcacaaa gagctcaaca ggttcaacag cctgctcaac aacctgctca acagaatacc
    20581 aatactgcaa atgcaggagg taaccaaaat caagcggctc cagtacaaaa ccaacctgtt
    20641 gctcaaccga ccgatattga tgggacttat actggtcagg atgacggaga ccgtatcact
    20701 ttagtggtaa ctggaacgac tggtacatgg actgagctcg aatctgacgg ggatcagaaa
    20761 gtcaaacagg ttacatttga ttcagcaaat caacgcatga ttattggcga tgatgtcaaa
    20821 atttacactg taaacggtaa tcaaatcgtc gtagatgata tggatagaga cccatcggac
    20881 caaatcgttt taactaaata agactatgca agtagaaagg gctggatttt tcagcctttt
    20941 tacttttacc tagaaaataa tcataaatac ttgccttcta ccgaatacct gcgttatact
    21001 agtatcaatt acttgttttt agcattcaaa ctatcaagga ggggatatga aatataggaa
    21061 atttcaatta ttgatgtcca agtatggctt tagtctttcg attatgctac ttgaactttg
    21121 tcttgttttt ggtctctttc tttatttagg acgcatggct cccattttat ggattactgt
    21181 cctcattcta ctgagtatca tcacaatcat ttcgatagtc aaccgtaata cgactcctga
    21241 gaataaggta acctggttgt tagtagcctt tgtgccagta tttggtccct tgctctatct
    21301 gatgtttggt gaaaggcgat tgtccaaaaa agaaatcaaa caactgaaga agctaggctc
    21361 tatgcatttc caagaagcaa atagccagct actaaaagag aaattaaaag aaagtgacaa
    21421 ggcagcttat ggagtcatca agtccttatt gagtatggat accaatgctg acatctatga
    21481 tcaaactgcc tctacatttt ttcctaacgg agaagctatg tggaaaaaga tggtagaaga
    21541 tcttaaaaag gctgagaaat ttattttctt ggaatattac attatagaag aaggtttgat
    21601 gtggaatcgc atactagata tactagagca aaaggtagct cagggtgtag aggttaagat
    21661 gctctatgat gatatcggct gtatggctac tttaacagga gattatgcac atcgacttcg
    21721 tcagctgggc atcgaggccc ataaattcaa taaagttatt cctcgtctga cagtggctta
    21781 taataacaga gatcatagaa aaatattgat tgttgatggt cagatagcct atactggtgg
    21841 ggtcaatctg gcagatgagt acattaacca cgtcgagaga tttggttatt ggaaggatag
    21901 tggaattcgc ttagacggac tagcagtaaa agccctgaca cgcttatttt tgaccacttg
    21961 gtacattaat cgaggagaaa ttagtgattt tgatcaatat catttagaaa atcattctat
    22021 cccgagtgac ggtttaacca ttccatacgg aagtggaccc aagccaattt ttcgagcgca
    22081 ggtagggaaa aaagtttatc agagtttaat caatcaagca acagaatcgg tctatattac
    22141 gacaccttat ttgattatag attatgattt aacagagaca atcaaaaatg cagctatgag
    22201 aggggtcgat gttcgaatta tcacccctta cataccagat aagaagttca ttcagttagt
    22261 cacgagagga gcttatcccg accttctttc tgctggtgtt cggatttatg agtatagtcc
    22321 aggttttatt catagtaagc agatgttggt agacgaagat tttgcggtgg tggggacaat
    22381 caatctcgac taccggagct tggtacacca ttatgaaaat gcagtcttac tctataaaac
    22441 tccttctata agggaaatcg cccgagattt tcgaaatata tttgcagatt ctcaggaagt
    22501 ctatcctcat tctatcaaaa cgagctggta tcaaaagctt gtaaaagaaa tcgcccagct
    22561 attcgcccct atcttataag aaatctagaa cttaggatac aaaccagacc tatttttctt
    22621 gtcttttacg aatagaaaga aagaggaggg gaattcatgc taactaagga agaagtgaat
    22681 gatatgattg agttcaaaca gacacattta caccgctttc gggaaattga aacctttgtg
    22741 aaactatgca aaaaatcact caaacagcca tcgcaagctg acaatcctag gactttttga
    22801 tatactaaga cagataaatt gaacaggaga aacgatatga gtgtgtatgg tagagtagaa
    22861 gaagttcata aggagaatcg tgaacctcta gaataccaaa tcgaacaaga atcgcatcat
    22921 cgtgaatcaa gtcgtcttcc tttggtgaaa attttactat ggagtacgct tgtaacgggg
    22981 ataactctag gagtaccgct attactcgac ttaatgagtg cacaagaagt gcaggatttt
    23041 tatgcaggtt gggcgcttca tcagacaggg aagatttaca gcgactatta tggaagtcaa
    23101 ggtttgcttt attatttgct gacttacgtg agtcagggcg gatttttctt tgccatcttt
    23161 gagtggttag ccttggtagc aggaggattt ttccttttta gatcagcgga caccttgaca
    23221 gagcaaggag accaagctgg acatctggtg actatttttt acatgctagt tacaggtctt
    23281 gcttttggtg gaggctatgc gactctttta gcgcttcctt tcttattcgc agcctttagt
    23341 ttagttgcgg cttacctaag caatccaagc catgataagg gatttgtacg gattgggcta
    23401 gctttggcag gcggattttt ctttgctccc ttatcatcgc tcctgtttat tgctgtagtg
    23461 agtttaggct tgttggtctt taaccttggg catagacgct ttgcgcatgg gttttatcag
    23521 tttcttgcag tggctttagg tttttcactt gtcttttatc caactgccta ctatagtgct
    23581 gcaacaggaa gttttgggga tgcgattagt ggtattcgtt atcctattga cagtattcgc
    23641 tttgatttta cttctaaaat tttagagaat atgttttttt atggcttgtt gtcccttggt
    23701 ttgggatttg tggtttgtat ctttttaggt ctctttcaat ccaaaccctt taaactatac
    23761 gtcatttcgg ttcctgcaag tcttgtggta attttaggtt tgattttact tttcttttca
    23821 caagagcctc tgcacgcttc ttatttgatg gtcgtcttcc ctgttttcct acttttattg
    23881 gtaaccaata ttaagagtca acagaggggg cgtagtgcta gaagaagcct aagagaaacg
    23941 ccagttagcc tatggagtcg tttcttcaaa ggaaatctat atctgctagt ttttgggttt
    24001 gtctatcttt tgtctgttcc ttttttgatg aagtttgtcc tttatccagt accttatcaa
    24061 gaacgtaatc gtcttgctga tttggtaaaa gaggagacaa atacggaaga tgctatctat
    24121 gcatgggatg atactgcgac tctttatcgt aagagtgagc gcttgtcgcc atcggcgatt
    24181 ttgtccccgt tgcactatac agcaactgag gaaaatcgta ataagttact taatgacttg
    24241 aaagaaaaac aacctaaggt gattgtggta aatgacaagg tggtagtctg gtctgaagtg
    24301 gaaacacttt taaaagaaaa ttaccaacaa gtaaagactg attactcaga gtttaaagtc
    24361 tataaaatta aataaccaaa tcaatatctt gtgtattttt aaaaatttta ggatttttaa
    24421 cacaagatat tgatttttct ttttagagtg gtataatact ttttagaaag aacattttag
    24481 aaaagagcat gcgtatgatt gcactagaag aaaaaattac aattttgcca actctcttcg
    24541 tcgagaaacg agatgggaga cgtgttgtat ttgatgtgga caagattgac aaggctctcc
    24601 acaaggcggc tgacaaggtt atggatgtga cacccctggt tgaaaaacgc ctcaatgatc
    24661 tgactgagcg aattattaca gaaattcata gtcgctttcc acagggaatt aagatttacg
    24721 aaattcaaaa tatcgtagaa catgaactcc ttgaagccaa agaatatgcg ctggctgagg
    24781 agtatattac ttatcggaca cagagggatt ttgagcgctc aaaagcgacg gatatcaact
    24841 ttagtattca taaacttctc aacaaagacc agacagttgt caatgaaaat gctaataaag
    24901 acagtgatgt ctttaacact cagcgtgatt tgacagcagg gattgttggg aaatcaatcg
    24961 gactgcaaat gcttcctaag cacgtagcca atgcccacca aaagggggat atccactatc
    25021 acgatttgga ctacagtccc tataccccta tgaccaactg ctgtttgatt gattttaagg
    25081 gtatgttgga aaatggtttt aagattggaa atgcagaggt agagagtccc aagtctatcc
    25141 agactgcgac agcacagatt tctcaaatca ttgccaacgt tgcttctagc cagtacggtg
    25201 gctgttcagc tgaccgtatc gatgaaattt tggcgcctta tgcagagaag aactatcaaa
    25261 aacatctcaa agatgcagaa gagtgggtat tgcctgaaaa acgggaagat tacgcttgga
    25321 agaaaacgca aaaggacatc tacgatgcca tgcaatctct tgagtatgaa atcaatactc
    25381 tcttcacttc aaatggacaa acacctttta cttcgttagg ttttggtctg ggaaccagtc
    25441 gttttgaacg agaaattcaa aaagctattt taaacattcg catcaagggt cttggttcag
    25501 aacaccgtac ggctatcttt cctaaactta tctttacgct taaaagaggc ctcaacttag
    25561 aggaaggaac tcccaactat gacatcaagc agttggctct agagtgtgca accaagcgga
    25621 tgtatccaga cgtcttgtct tatgataaga ttgttgattt gacaggttct ttcaaagttc
    25681 ctatgggctg ccgttctttc cttcaagggt ggaaggatga aaatggtgta gaagtcaatt
    25741 caggtcgcat gaatctgggt gttgtgacgg ttaatctgcc tcgtattgct cttgagtctg
    25801 aaggtgatat gaataagttc tgggaaatct tcaacgagcg aatgaatatc gcagaagatg
    25861 ctcttgttta ccgtgtcgaa cgcactaaag aggcgacacc agcgaatgct cctattcttt
    25921 atcagtacgg tgcttttggc catcgtctag gtaaagaaga aagtgttgac cagctcttta
    25981 agaatcgtcg tgcgaccgtt tcgctgggct atatcggctt gtatgaagta gcgacagttt
    26041 tctttggtaa cagctgggaa agtaatccag atgctaagga attcacgcta gacatcattc
    26101 acgatatgaa acgccgtgta gaagagtggt cagaccaata tggctaccat ttctctatct
    26161 actcaacacc atccgaaagt ctgacagacc gtttctgccg actagatata gacaagtttg
    26221 gctctattcc tgatatcaca gacaaggaat actacaccaa ctctttccac tacgatgttc
    26281 gtaaaaatcc aacaccgttt gaaaaattgg actttgagaa agtctatcca gaagcaggtg
    26341 cgtcaggtgg tttcatccat tattgtgagt atccagtcct tcagcaaaat ccaaaggcct
    26401 tggaagctgt ctgggattat gcttatgacc gtgtaggcta tctaggcacc aatactccga
    26461 ttgaccgttg ctacaagtgt gactttgaag gggattttga accaactgag agagggtttg
    26521 cttgtccaaa ctgtggcaat agcgatccta aaacagtaga cgttgttaaa cggacttgtg
    26581 gctatcttgg caatcctcaa gcgagaccaa tggttaacgg gcgtcacaag gaaatcgctg
    26641 cgcgtgtcaa acatatgaat ggttcaacga ttaaaatagc tgggcatcaa gtaacaaatt
    26701 agaaagaaat gaaatgggaa aatatcaact agacgataag gggcgcgcac aagtgacccg
    26761 ttatcacgag aagcactcta aaggtggagc tggtaagaaa gaacgcttgc ttagcttcag
    26821 agaacaattt ttaaacaaga acaagaaaaa ataaaagtga gagccagctc tcgcttttct
    26881 catagtggga ggtagggatg gaattacgca gaccaagatt agcggataag aaagctgttt
    26941 tagatatgat gacagagttt gaaaagagcc aatcagccca tgatggagga ttttgggata
    27001 cagagaattt tgtgtatgaa gagtggttgg aaagcaatca ggaacaggaa atggggatta
    27061 atctgcctga aggatgggtt cctgcaattc agttagtggc tttttctgag aaaggtcaag
    27121 cagttggatt tcttaatctc cggttgcgcc tcagtaactt tttactagaa gaaggtgggc
    27181 acattggcta ctccatccgt ccatctgaaa gaggcaaggg ttatgcaaaa gagactctcc
    27241 gtcagggctt gcaagttgct aaggaaaaga acatcaagaa agctctggtg acctgtagcg
    27301 tgaataatcc tgctagcaga gcagtcattc tagcaaatgg tggaatattt gaggatgctc
    27361 acaatggagt cgagcgttat tggatagagg tagcgaatga ataatccaaa accacaagaa
    27421 tggaaaagcg aggaacttag tcaaggtcgt atcattgact acaaggcctt taactttgtg
    27481 gacggcgaag gcgtgcgtaa ctctctctat gtatcaggct gtatgtttca ctgcgaggga
    27541 tgttataatg ttgcgacttg gtcttttaat gctggcattc cctatacagc agaattagaa
    27601 gagcagatca tggcagatct tgctcagccc tatgttcaag gtttgacttt gttgggaggg
    27661 gagccttttc ttaatactgg gattctcttg ccacttgtta agcggattcg gaaggaattg
    27721 ccagacaagg acatctggtc ctggaccggc tacacttggg aagaaatgat gttggaaact
    27781 ccagataaac tggaattctt gtcactgatt gacattcttg tcgatggaag atatgatcga
    27841 actaagagaa atcttatgct ccagtttcga ggttcatcta accaacgaat tatcgatgtg
    27901 caaaaatcgc tcaaaagtgg gcaagtagtg atttgggaca agctcaatga cggaaaagaa
    27961 agctatgaac aggtgaagag agaatgaaga aaaaagactt agtagaccaa ctagtctcag
    28021 agatcgagac ggggaaagtc aggacactgg gaatatacgg tcatggagct tcaggtaaat
    28081 caacctttgc acaggaattg taccaagctt tagattctac tacagtaaat ttgctagaaa
    28141 cagatcccta tatcacctcc ggacgctatt tggtagtacc caaggacgcg ccgaatcaaa
    28201 aggtgacagc cagtctgcca gtggcgcatg aactggagag tttgcagaga gatatccttg
    28261 ccttgcaggc gggtatggat gtcttgacaa ttgaagaacc ttggaaggct agtgaggtct
    28321 tgtctggagc caaaccaatt ttgattgtcg aagggatgtc tgttggtttt ctacccaagg
    28381 aactctttga aaaaaccatc tgtttctaca cggatgagga gaccgaatta aagcgacgcc
    28441 ttgctagaga tacgactgtg agaaatcgcg atgcatcctt tatattggct agccatcaga
    28501 tgagacggga gcagtatctg cgatactata aagaaactga gtctaaggcg gatatcttag
    28561 tggaccaatc agaagataaa tttgatgtca agaggactta acttatgtat agaagaaata
    28621 tagaagaaaa tccctaattt tagaaagaaa aagtaggaaa acagtttcat cccaaaaaaa
    28681 cgaaaaaacc taacaaatcc cttgcaatcg caggggattt gtgttattct attatggtgc
    28741 tgtaaattac agccttagtt ttgatgcaag aggttgcgac acgctcggtt gcattgccac
    28801 gcaacaccgc gtcggttttc ttgtggagct agcctattat cttaaataga cgaaaaggag
    28861 aaaaagatgg caaacaaaaa aatccgtatc cgtttgaaag cttacgaaca ccgtacgctt
    28921 gacacagcgg ctgcaaaaat cgtagaatca gctactcgta caggtgcaca agttgcgggt
    28981 ccaatcccac ttccaactga acgtagcctc tacacaatca ttcgtgcgac tcacaaatac
    29041 aaagactctc gcgaacaatt tgaaatgcgt acacacaaac gtttgatcga tatcgttaac
    29101 ccaactcaaa aaacagttga tgccttgatg aaattggatc ttccaagtgg tgtaaacgta
    29161 gaaatcaaac tttaatctaa aatataaaag agcagaggct ggtgtttcaa tctaattgaa
    29221 cacgggctag actcggtgtg aaaaagataa acttcctatt gtccgctaga cactgcgtta
    29281 gtttcctatt ttcactttga gtttgacgcc ctttgtatct tagacttgag cataaaaaac
    29341 gctcgttaaa aactttttga ataaaaaata tagaaaagga actattttct catgacaaaa
    29401 ggaatcttag ggaaaaaagt gggaatgact caaatcttca ctgaagctgg cgaattgatc
    29461 cctgtaacag ttattgaagc aactccaaac gttgttcttc aagttaaaac tgttgaaaca
    29521 gacggataca acgctatcca agttggtttc gatgacaaac gcgaagtatt gagcaacaaa
    29581 cctgctaaag gacatgtagc gaaagctaac acggctccta agcgcttcat tcgtgaattc
    29641 aaaaacgttg aaggcttgga agttggtgct gaaattacag ttgaaacatt cgcagctgga
    29701 gatgttgttg atgtaactgg tacttctaaa ggtaaaggtt tccaaggtgt tatcaagcgc
    29761 cacggacaat cacgtggacc gatggctcac ggttctcgtt accaccgtcg tccaggttct
    29821 atgggacctg ttgcacctaa ccgcgtattc aaaggtaaaa accttgcagg acgtatgggt
    29881 ggtgaccgcg taacaattca aaatcttgaa gttgtacaag ttgttccaga aaagaacgtt
    29941 atccttatca aaggtaacgt accaggtgct aagaaatctc ttatcactat caaatcagca
    30001 gttaaagctg gtaaataata aagaaagggg aaatcagtca caatggcaaa cgtaacatta
    30061 tttgaccaaa ctggtaaaga agctggccaa gttgttctta acgatgcagt atttggtatc
    30121 gaaccaaatg aatcagttgt gtttgatgta atcatcagcc aacgcgcaag ccttcgtcaa
    30181 ggaacacacg ctgttaaaaa ccgctctgca gtatcaggtg gtggacgcaa accgtggcgt
    30241 caaaaaggaa ctggacgtgc tcgtcaaggt tctatccgct caccacaatg gcgtggtggt
    30301 ggtgttgtct tcggaccaac tccacgttca tacggctaca aacttccaca aaaagttcgt
    30361 cgcctagctc ttaaatcagt ttactctgaa aaagttgctg aaaacaaatt cgtagctgta
    30421 gacgctcttt catttacagc tccaaaaact gctgaatttg caaaagttct tgcagcattg
    30481 agcatcgatt ctaaagttct tgttatcctt gaagaaggaa atgaattcgc agctctttca
    30541 gctcgtaacc ttccaaacgt gaaagttgca actgctacaa ctgcaagtgt tcttgacatc
    30601 gcaaatagcg acaaacttct tgtcacacaa gcagctatct ctaaaatcga ggaggttctt
    30661 gcataatgaa tttgtatgat gttatcaaaa aacctgtcat cactgaaagc tcaatggctc
    30721 aacttgaagc agggaaatat gtatttgaag ttgacactcg tgcacacaaa cttttgatca
    30781 agcaagctgt tgaagctgct ttcgaaggtg ttaaagttgc caatgttaac acaatcaacg
    30841 taaaaccaaa agctaaacgt gttggacgtt acactggttt tactaacaaa actaaaaaag
    30901 ctatcatcac acttacagct gattctaaag caatcgagtt gtttgctgct gaagctgaat
    30961 aatctaagga ggaaatatcg tgggaattcg tgtttataaa ccaacaacaa acggtcgccg
    31021 taatatgact tctttggatt tcgctgaaat cacaacaagc actcctgaaa aatcattgct
    31081 tgttgcattg aagagcaagg ctggtcgtaa caacaacggt cgtatcacag ttcgtcacca
    31141 aggtggtgga cacaaacgtt tctaccgttt ggttgacttc aaacgtaata aagacaacgt
    31201 tgaagcagtt gttaaaacaa tcgagtacga tccaaaccgt tctgcaaaca tcgctcttgt
    31261 acactacact gacggtgtga aagcatacat catcgctcca aaaggtcttg aagtaggtca
    31321 acgtatcgtt tcaggtccag aagcagatat caaagtcgga aacgctcttc cacttgctaa
    31381 catcccagtt ggtactttga ttcacaacat cgagttgaaa ccaggtcgtg gtggtgaatt
    31441 ggtacgtgct gctggtgcat ctgctcaagt attgggttct gaaggtaaat acgttcttgt
    31501 tcgtcttcaa tcaggtgaag ttcgtatgat tcttggaact tgccgtgcta cagttggtgt
    31561 tgtcggaaac gaacaacatg gacttgtaaa ccttggtaaa gcaggacgta gccgttggaa
    31621 aggtatccgc ccaacagttc gtggttctgt aatgaaccct aacgatcacc cacacggtgg
    31681 tggtgaaggt aaagcaccag ttggtcgtaa agcaccatct actccatggg gcaaacctgc
    31741 tcttggtctt aaaactcgta acaagaaagc gaaatctgac aaacttatcg ttcgtcgtcg
    31801 caacgagaaa taatattaaa ctagtcgctt aagcaactag taaatccgcc agctcggtag
    31861 cgctccatag gagtgcaagc cgctgtggta caacatttaa aggagaaaat ataaaaatgg
    31921 gacgcagtct taaaaaagga cctttcgtcg atgagcattt gatgaaaaaa gttgaagctc
    31981 aagctaacga cgaaaagaaa aaagttatta aaacttggtc acgtcgatca acgatcttcc
    32041 caagtttcat tggttacact attgcagttt atgacggacg taaacacgta cctgtttaca
    32101 tccaagaaga catggtaggc cacaaacttg gtgaatttgc accaactcgt acttacaaag
    32161 gtcacgctgc agacgacaag aaaacacgta gaaaataagg agaacataaa tggcagaaat
    32221 tacttcagct aaagcaatgg ctcgtacagt acgtgtttca cctcgtaaat cacgtcttgt
    32281 tcttgataac atccgtggta aaagcgtagc cgatgcaatc gcaatcttga cattcactcc
    32341 aaacaaagct gctgaaatca tcttgaaagt tttgaactca gctgtagcta acgctgaaaa
    32401 caactttggt ttggataaag ctaacttggt agtatctgaa gcattcgcaa acgaaggacc
    32461 aactatgaaa cgtttccgtc cacgtgcgaa aggttcagct tcaccaatca acaaacgtac
    32521 agctcacatc actgtagctg ttgcagaaaa ataaggaggt aaaatcgtgg gtcaaaaagt
    32581 acatccaatt ggtatgcgtg tcggcatcat ccgtgattgg gatgccaaat ggtatgctga
    32641 aaaagaatac gcggattacc ttcatgaaga tcttgcaatc cgtaaattcg ttcaaaaaga
    32701 acttgctgac gcagcagttt caactattga aatcgaacgc gcagtaaaca aagttaacgt
    32761 ttcacttcac actgctaaac caggtatggt tatcggtaaa ggtggtgcta acgttgatgc
    32821 actccgtgca aaacttaaca aattgactgg aaaacaagta cacatcaaca tcatcgaaat
    32881 caaacaacct gatttggatg ctcaccttgt aggtgaagga attgctcgtc aattggagca
    32941 acgtgttgct ttccgtcgtg cacaaaaaca agcaatccaa cgtgcaatgc gtgctggagc
    33001 taaaggaatc aaaactcaag tatcaggtcg tttgaacggt gcagatatcg cccgtgctga
    33061 aggatactct gaaggaactg ttccgcttca cacacttcgt gcagatatcg attacgcttg
    33121 ggaagaagca gatactacat acggtaaact tggtgttaaa gtatggatct accgtggtga
    33181 agttcttcca gctcgtaaaa acactaaagg aggtaaataa ccaatgttag tacctaaacg
    33241 tgttaaacac cgtcgtgagt tccgtggaaa aatgcgcggt gaagcaaaag gtggaaaaga
    33301 agtagcattc ggtgaatacg gtcttcaagc tacaactagc cactggatca ctaaccgcca
    33361 aatcgaagct gctcgtatcg ccatgactcg ttacatgaaa cgtggtggta aagtttggat
    33421 taaaatcttc ccacacaaat catacactgc taaagctatc ggtgtgcgta tgggatctgg
    33481 taaaggggca cctgaaggtt gggtagcacc agttaaacgt ggtaaagtga tgttcgaaat
    33541 cgctggtgta tctgaagaga ttgcacgtga agcgcttcga cttgctagcc acaaattgcc
    33601 agttaaatgt aaattcgtaa aacgtgaagc agaataagga gaaggcatga aacttaatga
    33661 agtaaaagaa tttgttaaag aacttcgtgg tctttctcaa gaagaactcg cgaagcgcga
    33721 aaacgaattg aaaaaagaat tgtttgaact tcgtttccaa gctgctactg gtcaattgga
    33781 acaaacagct cgcttgaaag aagttaaaaa acaaatcgct cgtatcaaaa cagttcaatc
    33841 tgaagcgaaa taatagacta gggaaggaga aatttcaatg gaacgcaata atcgtaaagt
    33901 tcttgttgga cgtgttgtat ctgacaaaat ggacaagaca atcacagttg tagttgaaac
    33961 aaaacgtaac cacccagtct atggtaaacg tattaactac tctaaaaaat acaaagctca
    34021 tgatgaaaac aatgttgcca aagaaggcga tatcgtacgt atcatggaaa ctcgcccgct
    34081 ttcagctaca aaacgtttcc gtcttgtaga agttgttgaa gaagcggtca tcatctaatc
    34141 aaacctgaaa ggagaaaact gaaatgattc aaacagaaac tcgtttgaaa gtcgcagaca
    34201 acagcggtgc tcgcgaaatc ttgactatca aagttcttgg tggttcagga cgtaaatttg
    34261 caaacatcgg tgatgttatc gtggcatctg taaaacaagc tactcctggt ggtgcggtta
    34321 aaaaaggtga cgttgttaaa gcagttatcg ttcgtactaa atcaggtgct cgtcgtgctg
    34381 atggttcata catcaaattt gacgaaaacg cagcagttat catccgtgaa gacaaaactc
    34441 ctcgcggaac acgtatcttt ggcccagttg cacgtgaatt gcgtgaaggt ggcttcatga
    34501 agatcgtgtc acttgctcca gaagtacttt aatttttagg aacaaactag tcccctagct
    34561 tcaagctagg gtgcccttat gggcgtaaga aaaatcaagg agaaacctaa tgtttgtaaa
    34621 aaaaggcgac aaagttcgcg taatcgctgg taaagataag ggaacagaag ctgttgtcct
    34681 tactgccctt ccaaaagtaa acaaagttat cgttgaaggt gttaacattg ttaagaaaca
    34741 ccaacgtcca actaacgagc ttcctcaagg tggtatcatc gagaaagaag cagctatcca
    34801 cgtatcaaac gttcaagttt tggacaaaaa tggtgtagct ggtcgtgttg gttacaaatt
    34861 tgtagacggt aaaaaagttc gctacaacaa aaaatcaggc gaagtgcttg attaatcacg
    34921 aaggaaagga gaagtataat ggcaaatcgt ttaaaagaaa aatatcttaa tgaagtagtt
    34981 cctgctttga cagaacaatt caactactca tcagtgatgg ctgtgcctaa agtagataag
    35041 attgttttga acatgggtgt tggtgaagct gtatcaaacg ctaaaagcct tgaaaaagct
    35101 gctgaagaat tggcacttat ctcaggtcaa aaaccactta tcactaaagc taaaaaatca
    35161 atcgccggct tccgtcttcg tgaaggtgtt gcgatcggtg caaaagttac ccttcgtggt
    35221 gaacgtatgt acgaattctt ggataaattg gtatcagttt cacttccacg tgtacgtgac
    35281 tttcacggtg ttccaacaaa atcatttgat ggacgcggga actacacact tggtgtgaaa
    35341 gaacaattaa tcttcccaga aatcaacttc gatgacgttg acaaaactcg tggtcttgac
    35401 atcgttatcg taacaactgc taacactgac gaagagtcac gtgcattgct tacaggcctt
    35461 ggaatgcctt ttgcaaaata atataggagg taaatctaat ggctaaaaaa tcaatggtag
    35521 ctagagaggc taaacgccaa aaaattgttg accgttatgc tgaaaaacgt gctgcattaa
    35581 aggcggcagg ggactacgaa ggtttatcta aattacctcg caacgcctca ccgactcgtt
    35641 tacataatcg ttgtagagtt acggggcgcc cacattcagt ttaccgcaaa tttggtctga
    35701 gtcgtatcgc ttttcgcgaa cttgcgcata aaggtcaaat tcctggtgta acaaaagcat
    35761 cttggtaatt taagatatca agggtgtcaa aactccaagt aaaaatagga aacttgacga
    35821 agaaactaaa gtttctagga aagtttatct ttttcacaca gagtttagcc cgggttcaat
    35881 tgggcttgcc aatttgaacc cgagctacag ctttggcaaa aaagaccaat ttgctttgga
    35941 gcattgcttc tgcattaaat tgtctatttt tgctcgtgct gttacgctct ttgtatcatg
    36001 tattaactag caagtgcaac ttgcaaacta ctagtaagag gagaaaaaca aaatggttat
    36061 gactgaccca atcgcagact tcctaactcg tattcgtaat gctaaccaag ctaaacacga
    36121 agtacttgaa gtacctgcat caaacatcaa aaaagggatt gctgaaatcc ttaaacgcga
    36181 aggttttgta aaaaacgttg aaatcatcga agatgacaaa caaggcgtca tccgtgtatt
    36241 tcttaaatac ggaccaaatg gtgaaaaagt tatcactaac ttgaaacgtg tttctaaacc
    36301 aggacttcgt gtctacaaaa aacgtgaaga ccttccaaaa gttcttaacg gacttggaat
    36361 tgccatcctt tcaacttctg aaggtttgct tactgataaa gaagcacgcc aaaagaatgt
    36421 tggtggtgag gttatcgctt acgtttggta aaatcaagat acaaagctcg taaagaacaa
    36481 agcaaaatta ggaagttgga gaagtttgtt tacaaacaag ccaacttatc tattttgcac
    36541 agttcttaga gcgtgttcag ttcagctctt gaactaaata agtatctgaa ccccgtgaaa
    36601 actggccgtt ctggcctgac aatttaacag gagaaaataa acatgtcacg tattggtaat
    36661 aaagttatcg tgttgcctgc tggtgttgaa ctcgctaaca atgacaacgt tgtaactgta
    36721 aaaggaccta aaggagaact tactcgtgag ttctcaaaag atattgaaat ccgtgtggaa
    36781 ggtactgaaa taactcttca ccgtccaaac gattcaaaag aaatgaaaac tatccacgga
    36841 actactcgtg cccttttgaa caacatggtt gttggtgtat cagaaggatt caagaaagaa
    36901 cttgaaatgc gtggggttgg ttaccgtgca cagcttcaag gatctaaact tgttttggct
    36961 gttggtaaat ctcatccaga cgaagttgaa gctccagaag gaattacttt tgaacttcca
    37021 aacccaacaa caatcgttgt tagcggaatt tcaaaagaag tagttggtca aacagctgct
    37081 tacgtacgta gccttcgttc accagaacca tataaaggta aaggtatccg ttacgttggt
    37141 gaattcgttc gccgtaaaga aggtaaaaca ggtaaataat gttgagtggt tgatcatcaa
    37201 ccaccaacct attttccaac tttgtgcata gcacacgatt taaaactaaa gaggtgaaaa
    37261 ctgtgatttc aaaaccagat aaaaacaaac tccgccaaaa acgccaccgt cgcgttcgcg
    37321 gaaaactctc tggaactgct gatcgcccac gtttgaacgt attccgttct aatacaggca
    37381 tctacgctca agtgattgat gacgtagcgg gtgtaacgct cgcaagtgct tcaactcttg
    37441 ataaagaagt ttcaaaagga actaaaactg aacaagccgt tgctgtcggt aaactcgttg
    37501 cagaacgtgc aaacgctaaa ggtatttcag aagtggtgtt cgaccgcggt ggatatctat
    37561 atcacggacg tgtgaaagct ttggctgatg cagctcgtga aaacggattg aaattctaat
    37621 aggaggacac tagaaaatgg catttaaaga caatgcagtt gaattagaag aacgcgtagt
    37681 tgctgtcaac cgtgttacaa aagttgttaa aggtggacgt cgtcttcgtt tcgcagctct
    37741 tgttgttgtt ggtgaccaca atggtcgcgt aggatttggt actggtaaag ctcaagaagt
    37801 tccagaagca atccgtaaag cagtagatga tgctaagaaa aacttgatcg aagttcctat
    37861 ggttggaaca acaatcccac acgaagttct ttcagaattc ggtggagcta aagtattgtt
    37921 gaaacctgct gtagaaggtt ctggagttgc cgctggtggt gcagttcgtg ccgttgtgga
    37981 attggcaggt gtggcagata ttacatctaa atcacttggt tctaacactc caatcaacat
    38041 tgttcgtgca actgttgaag gtttgaaaca attgaaacgc gctgaagaaa ttgctgccct
    38101 tcgtggtatt tcagtttctg atttggcata agaaagggga taaaatggct caaattaaaa
    38161 ttactttgac taagtctcca atcggacgca ttccatcaca acgtaaaact gttgtagcac
    38221 ttggacttgg caaattgaac agctctgtta ttaaagaaga taacgctgct atccgtggta
    38281 tgattacagc agtatctcac ttagtaacag ttgaagaagt aaactaatga agttttaggg
    38341 gatgtgcact gtaccatccc ctaaaactag atatagtcat ctatgatgac atcgtatagg
    38401 cgagttgatg ggggagacaa ccttttctcc cttatcggcg ctagcatttt acaaaagagg
    38461 agaaaataaa aatgaaactt catgaattga aacctgcaga aggttctcgt aaagtacgta
    38521 accgcgttgg tcgtggtact tcatcaggta acggtaaaac atctggtcgt ggtcaaaaag
    38581 gtcaaaaagc tcgtagcggt ggcggagttc gccttggttt tgaaggtgga caaactccat
    38641 tgttccgtcg tcttccaaaa cgtggattca ctaacatcaa cgctaaagaa tacgcaattg
    38701 tgaaccttga ccaattgaac gtctttgaag atggtgctga agtaactcca gttgttctta
    38761 tcgaagcagg aattgttaaa gctgaaaagt caggtattaa aattcttggt aacggtgagt
    38821 tgactaagaa attgactgtg aaagcagcta aattctctaa atcagctgaa gaagctatca
    38881 ctgctaaagg tggttcagta gaagtcatct aagagaggtg acctatgttt tttaaattat
    38941 taagagaagc tcttaaagtc aagcaggttc gatcaaaaat tttatttaca atttttatcg
    39001 ttttggtctt tcgtatcgga actagcatta cagttcctgg tgtgaatgcc aatagcttga
    39061 atgctttaag tggattatcc ttcttaaaca tgttgagctt ggtgtcgggg aatgccctaa
    39121 aaaacttttc gatttttgcc ctaggagtta gtccctatat caccgcttct attgttgtcc
    39181 aactcttgca aatggatatt ttacccaagt ttgtagagtg gggtaaacaa ggggaagtag
    39241 gtcgaagaaa attgaatcaa gctactcgtt atattgctct agttctcgct tttgtgcaat
    39301 ctatcgggat tacagctggt tttaatacct tggctggagc tcaattgatt aaaactgctt
    39361 taactccaca agtttttctg acgattggta tcatcttaac agctggtagt atgattgtca
    39421 cttggttggg tgagcaaatt acagataagg gatacggaaa cggtgtttcc atgattatct
    39481 ttgccgggat tgtttcctca attccagaga tgattcaggg catctatgtg gactactttg
    39541 tgaacgtccc aagtagccgt atcacttcat ctatcatttt cgtaatcatt ttgattatta
    39601 ctgtattgtt gattatttac tttacaactt atgttcaaca agcagaatac aaaattccaa
    39661 tccaatatac taaggttgca caaggtgctc catctagctc ttaccttccg ttaaaggtaa
    39721 accctgctgg agttatccct gttatctttg ccagttcgat tactgcagcg cctgcggcta
    39781 ttcttcagtt tttgagtgcc acaggtcatg attgggcttg ggtaagggta gcacaagaga
    39841 tgttggcaac tacttctcca actggtattg ccatgtatgc tttgttgatt attctcttta
    39901 cattcttcta tacgtttgta cagattaatc ctgaaaaagc agcagagagc ctacaaaaga
    39961 gtggtgccta tatccatgga gttcgtcctg gtaaaggtac agaagaatat atgtctaaac
    40021 ttcttcgtcg tcttgcaact gttggttccc tcttccttgg tgtgatttcc attttaccga
    40081 ttgcagctaa agatgtattt ggtctttctg atgttgttgc ctttggtgga acaagtctct
    40141 tgatcattat ctctacaggt atcgaaggaa tcaagcaatt ggaaggttac ctattgaaac
    40201 gtaagtatgt tggtttcatg gacagaacag aataaaagta tttactgaat cagtaaatac
    40261 tgagggagtg gaggtttaaa ctctgacatt tgtaagagtt ggatctcccc tcttctattt
    40321 tgtttttaaa taggggtgaa aagacttttt gcttctattt aaaaataaaa taaggagatc
    40381 aaatcatgaa tcttttgatt atgggcttac ctggtgcagg taagggaact caagcagcaa
    40441 aaatcgtaga acaattccat gttgcacata tctcaacagg tgatatgttc cgcgctgcaa
    40501 tggcaaatca aactgaaatg ggtgttcttg ctaagtcata tattgacaag ggtgaattgg
    40561 ttcctgacga agttacaaat ggaatcgtaa aagaacgcct ttcacaagat gatattaaag
    40621 aaacaggatt cttattggat ggttacccac gtacaattga acaagctcat gccttggaca
    40681 aaacattggc tgaacttggc attgaactag aaggtattat caatattgaa gtgaaccctg
    40741 acagcctctt ggaacgtttg agtggccgta tcatccaccg cgtaactgga gaaactttcc
    40801 acaaggtctt taacccacca gttgactata aagaagaaga ttactaccaa cgtgaagatg
    40861 ataagcctga gacagtaaaa cgtcgtttgg atgttaatat tgctcaagga gaaccaatca
    40921 ttgctcacta ccgtgccaaa ggtttggttc atgacatcga aggtaatcaa gatatcaatg
    40981 atgtcttctc agatattgaa aaagtattga caaatttgaa ataaagcgtt tttcgcactt
    41041 gcaaaaatcc gctacaaatg ttatactgag atagtctgac ttataattgt tgtctctgtg
    41101 tctagaggca tcgaatcgaa atttatggag gtgcttttgc gtggcaaaag acgatgtgat
    41161 tgaagttgaa ggcaaagtag ttgatacaat gccgaatgca atgtttacgg ttgaacttga
    41221 aaatggacat cagattttag caacagtttc tggtaaaatt cgtaaaaact atattcgtat
    41281 tttagcggga gatcgtgtta ctgtcgaaat gagtccatat gacttgacac gtggacgtat
    41341 cacttaccgc tttaaataat cgaaaaactt ggagggataa gaaatgaaag taagaccatc
    41401 ggtcaaacca atttgcgaat actgtaaagt tattcgtcgt aatggtcgtg ttatggtaat
    41461 ttgcccagca aatccaaaac acaaacaacg tcaaggataa gatagaaagg agaaaacatg
    41521 gctcgtattg ctggagttga tattccaaat gacaaacgcg tagtaatctc attgacttat
    41581 gtttatggta tcggacttgc aacatctaag aaaattttgg ctgctgctgg aatctcagaa
    41641 gatgttcgtg tacgtgatct tacatcagat caagaagatg ctatccgtcg tgaagtggat
    41701 gcaatcaaag ttgaaggtga ccttcgtcgt gaagtaaact tgaacatcaa acgtttgatg
    41761 gaaatcggtt cataccgtgg tatccgtcac cgtcgtggac ttcctgtccg tggacaaaac
    41821 actaaaaaca acgcccgcac tcgtaaaggt aaagctgttg cgattgctgg taagaaaaaa
    41881 taatatagga ggtaaaagtc ttggctaaac caacacgtaa acgtcgtgtg aaaaagaata
    41941 tcgaatctgg tattgctcat attcacgcta catttaataa cactattgtt atgattactg
    42001 atgtgcatgg taatgcaatt gcttggtcat cagctggtgc tcttggtttc aaaggttctc
    42061 gtaaatctac accattcgct gctcaaatgg cttctgaagc tgctgctaaa tctgcacaag
    42121 aacacggtct taaatcagtt gaagttactg taaaaggtcc aggttctggt cgtgagtcag
    42181 ctattcgtgc gcttgctgcc gctggtcttg aagtaacagc aattcgtgat gtgactccag
    42241 tgccacacaa tggtgctcgt cctccaaaac gtcgccgtgt ataatcatcg cattacactg
    42301 cttttcgttt aagagggagt aactaaatga tcgagtttga aaaaccaaat ataacaaaaa
    42361 ttgatgaaaa taaagattat ggcaagtttg taatcgaacc acttgaacgt ggctacggta
    42421 caactcttgg taactctctt cgtcgtgtac ttctagcttc tctaccagga gcagctgtga
    42481 catctatcaa cattgatggt gtgttacatg agtttgacac agttccaggt gttcgtgaag
    42541 acgttatgca aatcattctg aacattaaag gaattgcagt gaaatcgtac gttgaagacg
    42601 aaaaaatcat cgaactggat gttgaaggtc ctgctgaagt aacagctggt gacattttga
    42661 cagatagcga tattgaaatt gtaaatccag atcattatct ctttacaatt ggtgaaggtt
    42721 cttctctaaa agcgactatg actgttaaca gtggtcgtgg atatgtacct gctgatgaaa
    42781 ataaaaagga taatgcacca gttggaacac ttgctgtaga ttctatttat acaccagtta
    42841 caaaagtcaa ctatcaagtg gaacctgctc gtgtaggtag caatgatggt ttcgacaaat
    42901 taacccttga aatcttgaca aatggaacaa ttattccaga agatgcttta gggctttcag
    42961 cacgtatttt gacagaacat cttgatttgt ttacaaatct tactgagatt gctaagtcaa
    43021 ctgaagtgat gaaagaagct gatactgaat ctgacgaccg tattttagat cgtacgattg
    43081 aggaactgga cttgtctgtg cgttcataca actgtttaaa acgtgccggt atcaatactg
    43141 tgcatgattt gacagaaaaa tctgaagcag agatgatgaa agtacgaaat cttggacgca
    43201 agagtttgga agaagtgaaa ctcaaactca ttgatttggg tcttggatta aaagataaat
    43261 aaaggaggaa tacatggctt accgtaaact aggacgcact agctcacaac gtaaagcaat
    43321 gcttcgcgat ttgacaactg accttttgat caacgaatca atcgtgacaa ctgaagctcg
    43381 tgctaaagaa atccgtaaaa ctgttgaaaa aatgattact ctaggtaaac gtggtgattt
    43441 gcatgcacgt cgtcaagcag ctgctttcgt acgtaatgaa atcgcatctg aaaactatga
    43501 tgaagcaact gataagtaca cttctactac agcacttcaa aaattgttct cagaaatcgc
    43561 acctcgttat gctgaacgta acggtggata cactcgtatc cttaaaactg aaccacgtcg
    43621 tggtgatgca gcgccaatgg cgatcatcga attagtataa aatcatcaat tttgttgagt
    43681 gttatgatga tggagtcttg tgctcttagt ctagctctgg tctaccgcta ggatttcggt
    43741 cctagcggga acactcatca taagttggga tagtagacgc ttgtttacga aattgttttt
    43801 ttcttaagaa caacttcgta agcaggcgtt tttgagtatt ttcgttagaa ttatgctata
    43861 ctatttgaaa agaatcctgt ttaatgttaa ggtttcttat tttaagaaga attggagttt
    43921 acttatgaaa gcgattataa ctgttgttgg taaagataaa tctggaattg ttgcaggtgt
    43981 ttctggtaaa attgcagaat taggattgaa tattgacgat atctctcaaa ctgtattgga
    44041 tgaatatttt acgatgatgg ctgttgtatc tagtgatgaa aagcaagatt ttacctatct
    44101 tcgtaatgaa tttgaagctt ttgggcaaac tttgaatgta aaaatcaata ttcagagtgc
    44161 agcgattttc gaagctatgt ataatatcta ggaggtcatc atggatatta gacaagttac
    44221 tgaaaccatc gccatgattg aggagcaaaa cttcgatatt agaaccatta ccatggggat
    44281 ttctcttttg gactgtatcg atccaaatat caatcgtgct gcggagaaaa tctatcaaaa
    44341 aattacgaca aaggcggcta atttagtagc tgttggtgat gaaattgcgg ctgagttggg
    44401 aattcctatc gttaataagc gtgtatcggt gacacctatt tctctgattg gggcagcgac
    44461 agatgcgacg gactacgtgg ttctggcaaa agcgcttgat aaggctgcga aagagattgg
    44521 tgtggacttt attggtggtt tttctgcctt agtacaaaaa ggttatcaaa agggagatga
    44581 gattctcatc aattccattc ctcgcgcttt ggctgagacg gataaggtct gctcgtcagt
    44641 caatatcggc tcaaccaagt ctggtattaa tatgacggct gtggcagata tgggacgaat
    44701 tatcaaggaa acagcaaatc tttcagatat gggagtggcc aagttggttg tattcgctaa
    44761 tgctgttgag gacaatccat ttatggcggg tgcctttcat ggtgttgggg aagcagatgt
    44821 tatcatcaat gtcggagttt ctggtcctgg tgttgtgaaa cgtgctttgg aaaaagttcg
    44881 tggacagagc tttgatgtag tagccgaaac agttaagaaa actgccttta aaatcactcg
    44941 tatcggtcaa ttggttggtc aaatggccag tgagagactg ggtgtggagt ttggtattgt
    45001 ggacttgagt ttggcaccaa cccctgcggt tggagactct gtggcacgtg tccttgagga
    45061 aatggggcta gaaacagttg gcacgcatgg aacgacagct gccttggccc tcttgaacga
    45121 ccaagttaaa aagggtggag tgatggcctg taaccaggtc ggtggtctat ctggtgcctt
    45181 tatccctgtt tctgaggatg aaggaatgat tgctgcagtg caaaatggct ctcttaattt
    45241 agaaaaacta gaagctatga cggctatctg ttctgttgga ttggatatga ttgccatccc
    45301 agaagatacg cctgctgaaa ctattgcggc tatgattgcg gatgaagcag caatcggtgt
    45361 tatcaacatg aaaacaacag ctgttcgtat cattcccaaa ggaagagaag gcgatatgat
    45421 tgagtttggt ggtctattag gaactgcacc cgttatgaag gttaatgggg cttcgtctgt
    45481 cgacttcatc tctcgcggtg gacaaatccc agcaccaatt catagtttta aaaattaaga
    45541 aaataggaga aattttaagt tctatttaag attagatgtg tatactataa tcattaaata
    45601 aagacctcct aatattattt gaaacagata acactgaatt agtttgaatt tgattttcat
    45661 ctaatatctt tatttaatga actcctaaac tttttcataa taatctcctt caaaagtcgc
    45721 ctgtatgggt ggcttttatt ttatcattca tgatataata gaagcaaacg gaggacggaa
    45781 aatggtaaaa gtacgattgt atttggtacg tcatggcaag accatgttta acacgattgg
    45841 tcgcgcgcaa ggttggagcg atactccctt aactgctgaa ggtgaacgag ggattcaaga
    45901 gttaggaatc ggtttgcgag aatctgatct acagtttgag cgcgcttatt cgagtgattc
    45961 tggtcgtacc attcagacca tgggaattat acttgaagaa cttggcttgc aggggaaaat
    46021 cccttatcgc atggacaagc gtatcagaga atggtgtttc ggtagttttg atggagccta
    46081 tgatggcgat cttttcatgg gcattattcc tcgtatcttt aatgtggacc acgttcacca
    46141 attgtcttat gctgaactgg ctgagggctt ggtagaggtc gatacagctg gttgggctga
    46201 aggttgggaa aaactcagtg gccgaatcaa ggaaggcttt gaaataattg caaaagaaat
    46261 ggaagatcaa ggtggaggta acgcccttgt tgtcagccat ggaatgacta ttggaaccat
    46321 tgtttatctg attaatggca tgcatccgca tggtctagat aatggtagcg tgacgattct
    46381 tgaatatgag gacggtcagt ttagggttga agttgtcggt gaccgtagtt accgagagct
    46441 aggacgtgag aagatggaag aaggttctat ttaatcagtc tagacttgct tgccatgagc
    46501 tagggatttg ataagaatat caagataaga aaaaacagcc gagggtactc ctttcggctg
    46561 tttttgatgt ggaaaactaa agtgtaatgc tattgctttt agagattttc ataaacaaga
    46621 gcaaggaacc tactgttaga acagtcagga tagttgacaa ggctgcagct acaccgtaat
    46681 ttcctctgag aacctctgta taaatagcta cagtcattgt tcttgttttg acattgtaga
    46741 ggaggataga agtagagagt tctgaaatca gtgtgaccca agataggatg gctccagaaa
    46801 taatatccga tagcatcatt ggagttgtaa tcttagcaaa gatattgaga cgactacttc
    46861 ctaagctttc agcagcttct tcaatacttg gtgctatttg ttgtaagcta gcaacagatg
    46921 agcgaatagt ataaggtaat tttctggcag atagagacat aatcaagatg aaagcagtcc
    46981 ctgtaatcat aagaaatcca cttccaaata gaccagagaa atgaaggcaa tccctagaac
    47041 ggttcctggt acaatataag gtaccatact gaggctgtca attaagtttg taaacaaatt
    47101 ccgttttcta acggctaggt aggagataaa tgtcgtaaat agaacaacta gaactaaggc
    47161 aatcaaaggg atacgaatgg tattgaaaat agcagatccc atacgattga aagctagctt
    47221 gtaactgttt ggagaataac ctttaacaaa taccatacct gatgttttta ggaaagaggt
    47281 ataaattaag tagatttgag gtaaaacaga gataaagata attccgtaga ctgttgcata
    47341 aatggcagcc atttttcctt ttgtagtttt tttaggctca attggatgga gcagattcat
    47401 gctgaaactg tagcggtttg caatgtgttt ttggataagg aaaattgcca aggcaatgat
    47461 aatcgccata attgcaaaag cagaatttcc tccaacctcg ctaataaatt gggtataaat
    47521 caggacaggg aaagtccgat acccttcgcc aatcaacata ggcgttccaa agtctgagaa
    47581 tgctctcata aatacaagca aggagctgct agtaaggttg gaactaggag aggtaaaaca
    47641 accgttacga taggtttaaa tccgaaggac cccatgcttt cagctgcttc aagtagagaa
    47701 ttgtcaatac tgttcattgt tccagcaaca tatagaaata ccagtgggaa tagttgcagt
    47761 gtaaagacaa gtacaattcc tttgaatcca taaatatcga tagctggaag ataaagggca
    47821 tttgtcaaaa atttagtgat gacctcattt cgtcccagca agagaatcca ggagtaggct
    47881 cctacgaaag gagctgacat ggaagcaatg ataatcaata tttgtagaaa tttcttcccc
    47941 ttgaagtcat acatagagaa gagataagct aatagggttc ctacaactaa ggaagtgaca
    48001 gtagcggtaa tggaaaccct gaaactgttg actagtgtct cagagtagta ggctttacta
    48061 aagaaagtga caaaattagc tagtgagaat tgtccttcat gtataagtgc ttgcttgagc
    48121 acggtaacga taggataaac gagaaagaca agataggtaa gaaagaggaa gaaagaggag
    48181 gctgtccaaa tatttagttt tttacattcc atggttgact ccttttatca ggttttggga
    48241 accatctgca gaaaagatgt ttaatttttg cgtattgatt cgtagacgaa tacgattgcc
    48301 tttttgtaga tcttcttcaa aagttgattc ttcactaact tgaatttttg aggcaaaacc
    48361 tgtctcaatg aaatactccg tatttagtcc aagatagacg ctatcgctaa tagttccttc
    48421 gatatctcca gattcatctt tgataaactc ttcgggacga atgcttacat gaatagcttg
    48481 ctcctcaacc tgatcaagag ctagcattcg aagggcatag ccatctgaaa agacgatata
    48541 agcgccgtcg ctccgttttt caagattggc agggataata tttgtgcgtc cgataaaggt
    48601 tgccacaaac tcattagctg gtttatgata gagttctttt ggtcggccga tttgttggat
    48661 caccccatct ttcataacag caatttggtc tgaaatagcc atggcttctt cttggtcgtg
    48721 ggttacataa acagttgtaa ttcccacttc gtgttggatt tctcggatgg tttgacgcat
    48781 atccaagcga agtttggcct ccagattact aagtggctcg tccatgagga gaacacttgg
    48841 attaaccgct aaggcgcatg ccaaggtgac acgttgttgt tgtccaccac tgagtttatc
    48901 gggctttcga tccgcatatt gagcaatttg catgagttca agatacttgt tggtctgttg
    48961 aatcaattct tcttttggaa ccttcttttg cataagacca aaagcaacgt tgtctcggac
    49021 agtcaaatgt gggaaaatag cgtagttttg gaaaaccagc ccgatattgc gtttgctggg
    49081 ttccatatta ttgatttttg tatcatcgaa gtaaaattct ccatctttga tactgttgaa
    49141 acctgcaatc atacgaagaa gggtcgtttt cccacatcct gaagctccaa gaagggtaaa
    49201 gagacttcct tttggaattg taatgttcaa attctcaata acagggacat cgtggtagat
    49261 ttttttggcg ttaataattt cgatctcact catagtgaac ctcttttact gtttagattg
    49321 gatatctgta aagacttcgt tgtatttctt aacgatatct gatttattct tgatgacata
    49381 atcataatct taagtgagtg ttttgatttt gtcaattggt ttcatgtttt cgcttgtttt
    49441 agcattttta cgaacaggac ggttagtagt ggttgtacca agtgtatctt gtacttcttg
    49501 agagataata aaatcgataa atttcttggc attttccata tttttagatt ttttaacgat
    49561 agcagcacta gaaggtagga agacggttcc ttcttttgga tagactacct taatgttagc
    49621 tccgtcattt aagagtttaa ctgctggatc ttcataagag agaccaacag tcatttctcc
    49681 atcagcgact gctttataga caccagatga acttgaaccg at
//
LOCUS       DAVMEI010000003        35527 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVMEI010000003 DAVMEI010000000
VERSION     DAVMEI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.003"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(121..1755)
                     /locus_tag="VYQ24_000131"
     CDS             complement(121..1755)
                     /locus_tag="VYQ24_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967325.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEGMPIYFSSMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEDKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1736..2485)
                     /locus_tag="VYQ24_000132"
     CDS             complement(1736..2485)
                     /locus_tag="VYQ24_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967326.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2664..4118
                     /locus_tag="VYQ24_000133"
     CDS             2664..4118
                     /locus_tag="VYQ24_000133"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1967327.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4208..6826)
                     /gene="alaS"
                     /locus_tag="VYQ24_000134"
     CDS             complement(4208..6826)
                     /gene="alaS"
                     /locus_tag="VYQ24_000134"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1967328.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESAFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6848..7333)
                     /locus_tag="VYQ24_000135"
     CDS             complement(6848..7333)
                     /locus_tag="VYQ24_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1967329.1"
                     /translation="MKTFLVKQKFRLGGERFAIKNDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7608..7847)
                     /locus_tag="VYQ24_000136"
     CDS             complement(7608..7847)
                     /locus_tag="VYQ24_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967330.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8108..9178)
                     /locus_tag="VYQ24_000137"
     CDS             complement(8108..9178)
                     /locus_tag="VYQ24_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1967331.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9175..9948)
                     /locus_tag="VYQ24_000138"
     CDS             complement(9175..9948)
                     /locus_tag="VYQ24_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967332.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9945..10751)
                     /locus_tag="VYQ24_000139"
     CDS             complement(9945..10751)
                     /locus_tag="VYQ24_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967333.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10732..11889)
                     /locus_tag="VYQ24_000140"
     CDS             complement(10732..11889)
                     /locus_tag="VYQ24_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967334.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12049..12954)
                     /gene="murB"
                     /locus_tag="VYQ24_000141"
     CDS             complement(12049..12954)
                     /gene="murB"
                     /locus_tag="VYQ24_000141"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1967335.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13066..13818)
                     /locus_tag="VYQ24_000142"
     CDS             complement(13066..13818)
                     /locus_tag="VYQ24_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1967336.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13828..14559)
                     /gene="budA"
                     /locus_tag="VYQ24_000143"
     CDS             complement(13828..14559)
                     /gene="budA"
                     /locus_tag="VYQ24_000143"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1967337.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14701..15972
                     /locus_tag="VYQ24_000144"
     CDS             14701..15972
                     /locus_tag="VYQ24_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1967338.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15992..16807)
                     /locus_tag="VYQ24_000145"
     CDS             complement(15992..16807)
                     /locus_tag="VYQ24_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1967339.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(16964..17617)
                     /gene="phoU"
                     /locus_tag="VYQ24_000146"
     CDS             complement(16964..17617)
                     /gene="phoU"
                     /locus_tag="VYQ24_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1967340.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17629..18387)
                     /gene="pstB"
                     /locus_tag="VYQ24_000147"
     CDS             complement(17629..18387)
                     /gene="pstB"
                     /locus_tag="VYQ24_000147"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1967341.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18400..19203)
                     /gene="pstB"
                     /locus_tag="VYQ24_000148"
     CDS             complement(18400..19203)
                     /gene="pstB"
                     /locus_tag="VYQ24_000148"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1967342.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19214..20098)
                     /gene="pstA"
                     /locus_tag="VYQ24_000149"
     CDS             complement(19214..20098)
                     /gene="pstA"
                     /locus_tag="VYQ24_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1967343.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20088..21005)
                     /gene="pstC"
                     /locus_tag="VYQ24_000150"
     CDS             complement(20088..21005)
                     /gene="pstC"
                     /locus_tag="VYQ24_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1967344.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21005..21883)
                     /gene="pstS"
                     /locus_tag="VYQ24_000151"
     CDS             complement(21005..21883)
                     /gene="pstS"
                     /locus_tag="VYQ24_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1967345.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22457..23761)
                     /locus_tag="VYQ24_000152"
     CDS             complement(22457..23761)
                     /locus_tag="VYQ24_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1967346.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23762..24535)
                     /locus_tag="VYQ24_000153"
     CDS             complement(23762..24535)
                     /locus_tag="VYQ24_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1967347.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24525..24803)
                     /locus_tag="VYQ24_000154"
     CDS             complement(24525..24803)
                     /locus_tag="VYQ24_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1967348.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24803..25204)
                     /locus_tag="VYQ24_000155"
     CDS             complement(24803..25204)
                     /locus_tag="VYQ24_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1967349.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25414..26031)
                     /locus_tag="VYQ24_000156"
     CDS             complement(25414..26031)
                     /locus_tag="VYQ24_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1967350.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(26021..26794)
                     /locus_tag="VYQ24_000157"
     CDS             complement(26021..26794)
                     /locus_tag="VYQ24_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1967351.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTIYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26805..27542)
                     /locus_tag="VYQ24_000158"
     CDS             complement(26805..27542)
                     /locus_tag="VYQ24_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1967352.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27547..28677)
                     /locus_tag="VYQ24_000159"
     CDS             complement(27547..28677)
                     /locus_tag="VYQ24_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1967353.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28761..29150)
                     /locus_tag="VYQ24_000160"
     CDS             complement(28761..29150)
                     /locus_tag="VYQ24_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1967354.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29166..29549)
                     /locus_tag="VYQ24_000161"
     CDS             complement(29166..29549)
                     /locus_tag="VYQ24_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1967355.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29550..30338)
                     /locus_tag="VYQ24_000162"
     CDS             complement(29550..30338)
                     /locus_tag="VYQ24_000162"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1967356.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEK"
     gene            complement(30331..31266)
                     /locus_tag="VYQ24_000163"
     CDS             complement(30331..31266)
                     /locus_tag="VYQ24_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1967357.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31396..31572)
                     /locus_tag="VYQ24_000164"
     CDS             complement(31396..31572)
                     /locus_tag="VYQ24_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1967358.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31712..32419)
                     /locus_tag="VYQ24_000165"
     CDS             complement(31712..32419)
                     /locus_tag="VYQ24_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1967359.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32570..33598
                     /gene="queA"
                     /locus_tag="VYQ24_000166"
     CDS             32570..33598
                     /gene="queA"
                     /locus_tag="VYQ24_000166"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1967360.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34259..34648)
                     /locus_tag="VYQ24_000167"
     CDS             complement(34259..34648)
                     /locus_tag="VYQ24_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1967361.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(34702..35393)
                     /locus_tag="VYQ24_000168"
                     /pseudo
     CDS             complement(34702..35393)
                     /locus_tag="VYQ24_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
BASE COUNT        10556 a         8138 c         6737 g        10096 t
ORIGIN      
        1 cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca
       61 ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca accaactatc
      121 tcatcttttt tctatttctg ccaatatgcg tgacaggtaa taatgatagc caaaaatagc
      181 aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga taggggagag
      241 agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa
      301 caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca
      361 attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc
      421 aaggatccat gccacaataa ggtaaatcat cgaaatgatg ggcaagccta gatatagaga
      481 aagaccaagt aaagtcagaa ttggtaaaaa ggactggacg gcatatataa tccaaaattt
      541 cactttcaca taacgagcaa agtcaaaggg taaactctta agaaaatcaa cattttccct
      601 ctccaaggac aaagcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa
      661 cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga
      721 atctcggatt ttggaaatca gactgatcat catgagataa ggaaggaaag cacttgtaaa
      781 aagcactgta atcacaccag tcccctgtcc caagagggtg aggtggtagc gtaaaaccat
      841 acggaaaaag ccctttttag tagttgaaat cctctccttg ctgcgacgtt cttttttgac
      901 cttatcctca ctattaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt
      961 cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccacta aggcttctgt
     1021 cgaaaaaggc tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat
     1081 gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga taaatattaa
     1141 aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat actttctgaa
     1201 gaccctagtt tgagccaaga aatgcactgc cactaccatc actagagcca cagagacaaa
     1261 taataaggtc aaggacagta gcatcaaagg caaacccagc catagagaag gagctagcct
     1321 aatgtagagg accagaaaat aagctaggat tggtacaatt ccagttagag ctggcaaaag
     1381 gacagacagt cctttagcaa ttataatctc tgattcttta aaggcatagg gcctatacga
     1441 tatcaaatcc ttactctcat aaaagacatt gtaaaaggcc gttaaagaag ttgaaaaggc
     1501 aatcactagt aaaatagcaa tcatcgagct aaaataaata ggcattccct caaaaggaaa
     1561 atgaatggct atattactaa aacagatgag catcaagaga ctggaaaaaa tgtaagaact
     1621 taagactcta gcggaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag
     1681 attagccaga ttagcttctt gagatgaata aaggatattg atatcaacta attttttaat
     1741 gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg tagatacttt
     1801 ccaaagactg gtctgggtaa tcttttctca agtcctctac actaccacaa taaatcaaat
     1861 gccccttttt caaaatggca atccgatcac agacttgctc tgccacctct aggacatgag
     1921 ttgaaaacaa gactgtcttc cctttttgtg catgttcctt catcatctgt ttcaaatcaa
     1981 aggcagcctg gggatccaaa ccagtcaagg gttcatccaa gacccaaata tcgggatcag
     2041 acaagagtgc tccgatgaca aagactttct gacgcattcc gtgagaaaga gtttcaataa
     2101 cctgatagcg attttcagca aaatcaaaaa cgttcaatag cctagctaga ctagcctcca
     2161 agtcagatct actcagatca taggatgagg cgatcaattc ccaaaattca ttggccgtta
     2221 agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgttta atagccaagc
     2281 gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt gaaatgatac
     2341 tgactaggga ttttatggtg gtcgattttc cagccccatt atgaccaatc aagcccataa
     2401 tctctccatt ttcaatctgc aaattgagat tactcaaggc ctctttatca ccatacaact
     2461 tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta ttatacatcg
     2521 ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt aaaatagttt
     2581 ccttctatct tacgcaaacg tttgcgccga gagatacttt atgatagaat aaagaacaag
     2641 attgacaagt aagaggaaac atcatgcaaa atcaaacact catgcaatac tttgaatggt
     2701 atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca cacctagctc
     2761 atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac gaaaaagatg
     2821 tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa gggactgtcc
     2881 gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt aaagcacagg
     2941 gaattcaacc tatggccgat gtagttctca accacaaggc tgctgccgat cacagggaag
     3001 cctttcaggt tatcgaagtt gatcctgtag accgtacagt tgaacttgga gaacccttca
     3061 ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctat aatggcttcc
     3121 actggcattg gtaccacttc accggtacag actacgatgc caaacgcagt aaatctggga
     3181 tttatctgat ccaaggggac aacaagggct gggctaacga ggaattggtc gataacgaaa
     3241 acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc
     3301 aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt
     3361 tggatgccgt taagcatatt gactctttct ttatgcgcaa cttcatccgc gatatgaagg
     3421 aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca
     3481 atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccatc
     3541 agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag
     3601 atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc
     3661 aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc
     3721 tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt
     3781 caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta gccatccgaa
     3841 aagacttggc ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg
     3901 tacgttcagg tgctgaaaat caatccccaa tcgcagtcct tatctcaaat gaccaagaaa
     3961 acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg
     4021 gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag
     4081 ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag
     4141 gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt
     4201 tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta gcttggttgc
     4261 ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta
     4321 attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga
     4381 ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt tgtttccagt
     4441 tatctgcaaa agtacgaagg gcaccggcat cggatacaga cacttgacta gcaatgtaac
     4501 gatgaccgtt gacttcctta acatctttga agatatcgcc tgcggctgca gctgcggctt
     4561 tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta
     4621 ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca gctttaagag
     4681 cgtcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacggc
     4741 gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt
     4801 tgccaacgtg ggtaccacca caaagttcaa tagagtagtc accgatagtc acgacacgaa
     4861 cttccttgcc gtatttctca ccaaagaggg ccatcgctcc catttcttta gcagtgtcaa
     4921 tatccgtttc aactgtcttc acttcaagtg cttcccaaat tttctcgtta acttgctgtt
     4981 caatcgcacg aagttcctca gcagttactg cttggaagtg ggtaaagtca aagcgaagga
     5041 attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg
     5101 cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat
     5161 caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa
     5221 gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaacc ttacctgact
     5281 catccaagat ttgtccgtag tcagctacct gtccacccat ttcagcataa aatgacgttt
     5341 ccgcaaagat aagagaggca gttccttctg aaacagctcc tacttctgca ttgtccgcca
     5401 cgatagctac caatttagaa gacaattggc tagcattgta gttgaaagca ctttctacag
     5461 tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca gctgacgcac
     5521 gcgcgcgttc ttgctgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac
     5581 cagcctcttc agcaatttct tcagtcaatt caactgggaa tccgtatgta tcatagagtt
     5641 taaagacatc ttgaccagca atgacagatt gacctttttc tttcaagtct gccacaatac
     5701 cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttga
     5761 cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt
     5821 ttccaacagt tggaacgagt ttgtaaagga aaggctcgtt gattcccaat ttttgaccat
     5881 gcatagaagc acgacggaga agacgacgaa gaacataacc acgaccttca tttcctggaa
     5941 gggcaccatc accgatagca aatgaaagag aacgaatgtg gtctgcgata accttgaagc
     6001 tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa
     6061 tcggcataaa gaggtccgtt tcaaagttgg tcttagcccc ttggataaca gccaccaaac
     6121 gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat tcgctacgag
     6181 gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttca atataacggt
     6241 cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc
     6301 cacggtcaaa gaagatttct gtatctggtc cagaaggtcc cgcaccgatt tcccagaagt
     6361 tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag
     6421 aatctttatc gtctggatag taggtcatgt aaagtttttc agcagggaaa tcaaaccatt
     6481 cagggcttgt caaaagctca taagcccaag tgatagcttc gtcacggaag taatccccga
     6541 tagagaagtt ccccagcatt tcaaacatgg tatggtgacg cgcggtcttc cctacgtttt
     6601 cgatgtcgtt ggtacggata gccttttggg cattggtaat acgtggattt tcagggataa
     6661 tggtcccgtc aaagtatttc ttaagggttg ctaccccaga gttgatccac aaaagagttg
     6721 ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg gtcgcccaga
     6781 aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt
     6841 cacttgttta atgtgattgg ctttccaaca tttccacata gtcaatcgcg acacagaggg
     6901 aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta gaggtcagat
     6961 ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc aatttgaaat
     7021 tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc
     7081 gccagaaggt caacttctta cgaatgacaa aactcgagcc atctcgaagc tgaatctcaa
     7141 aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca ccagccgcat
     7201 catagatggt aaaggtttta ggaatcttaa aaaatgatcc ctccacctga taggcaattt
     7261 ctcccctgtc attcttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa
     7321 gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa
     7381 aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta
     7441 tgattgagta gcatgacttc ctagcttaga tggctcgcag caccgccatt tctctggact
     7501 aagacaagtg aaaatcaatt ctcaactttc ttattataac gtttttttaa gcttgcgtca
     7561 actggaaatg atctccgttg aattagacca attccctaca tctctgatta ctttttcagg
     7621 atatattttt tcttactgcc gtttttcttt ttatcccaaa ttttcatatt actaaacaca
     7681 gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt
     7741 aggtattgtc gatccaagcc atcctttaaa tggaatagta taacagtttg gttaacaatc
     7801 ataaaggttg gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc
     7861 tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc
     7921 tgtacttgag tacggcaagg caacgctgac gtggtttgaa gagtataggc ttagtatact
     7981 actaggcaag caaataagca aataaacaaa taaacaacta gaatagaaaa agatagggct
     8041 ctaaaaactg acttctattc cttaaaaacg aaccagcttg actgattcgt cttcttacgt
     8101 ttatctccta cttccgatac attttaaact gtaggaagag gtcgctatat ttccctgtcc
     8161 atttatggtc aaatttctca taaacttcta ggtgtttcat ggtttcaaca tcgggataga
     8221 aggccttatc ttcctttgtt tcctctggga gcaattcctt cgctggtagg tttggtgttg
     8281 aatagccgac atactccgca ttttggagag cattttcagg tttcaacata aagttgataa
     8341 aggcataggc tgagttttgg tttttaactg ttttgggaat gaccatattg tcaaaccaaa
     8401 gattgctggc ctctgtcggt accacataac gtagattttc atttttttct aacatttggc
     8461 tggcttcacc agagaaggtc acgccgattg caacattatt ctgaatcata tagcccttca
     8521 tctcgtccgc aacgatagcc ttgatatttg gagtcagttt gtagagctta tccactgtct
     8581 cttccaactg ctgcagatcc ttggagttga ggctgtagcc gagggaattg agtcctagtc
     8641 ccagcacctc acgcgcccca tcaaagagca tgatagaatt cttatactcc ggcttccaaa
     8701 ggtcatccca atgctcaggc gcttcatcta ccatggtttc gttgtagaca attcctaagg
     8761 ttccccagaa gtaagggatg gagaatttat tacctgggtc aaaggactgg ttgagaaact
     8821 ctggtccgat attttcgatt cctttaattt ttgaataatc aagcggaacc aagaggtctt
     8881 cgtccttcat cttgttaatc atgtattcac ttggaatggc aatatcgtag gtcgttccac
     8941 cctgctttat cttagtgtac atggcttcgt tggagtcaaa agtctcgtac tgaacttgaa
     9001 ttcctgtttc ttctgtaaac tgagtcaaga gttcaggatc gatatagtct ccccagttat
     9061 agataaccaa tttttgacta tctcgactat tgattttact atctaaatga gtcgcaattc
     9121 cccacaagac aaggataatc gctgcaattc ctgctaaaaa tgaatagatt tttttcatgc
     9181 ttgctcctcc ttctcacgag agataaagta ataacctaca actaggataa tactaaagag
     9241 aaagactaga gcagacaggg cattgatttc taaggaaatc cccttgcgag cacgagagta
     9301 aatctcgact gatagggttg aaaagccatt tcctgttaca aagaaggtca cggcaaaatc
     9361 atccagcgaa taggtgaagg ccatgaagta acctgcaatg atagacggag tcaggtaagg
     9421 aagcatgatt tccttaaaca tctgaaattg actagctccc aagtcatagg ccgcatgaat
     9481 catgtcgcca ttcatttcct tgagtcgagg caagaccatc aagaccacga taggaatgga
     9541 gaaggccacg tgactagata gaacggtcaa aaagccaagt gaaaacttga gttgggtaaa
     9601 gagaatcaag aagctagcac caatcataac gtcaggcgca accatgagga tattattgag
     9661 tgatagaaag gcttcttggt atttcttacg agactggtag atgtaaatgg caccaaaagt
     9721 cccgataatg gtcgctatca aggctgatag gaaggccaag aaaaatgtct gagccaaaat
     9781 cagcatgagt ctcccatctc caaacatggt ttcaaagtga gtccagctaa aacctgtaaa
     9841 gctattcata tcatcaccag cattaaaggc atagccaatc aagtaaaaaa tcggcaggta
     9901 gaggaccaga aagaccagtc ccagataaag gttggcaaat tttttcatcg ttctctcctt
     9961 tccttggtca cccacatggt gatgaacatg gtcaggatga gaatcacacc gatggttgaa
    10021 cccataccat agttgtcatt ggttagaaaa ttctgctcaa tagccgtccc caaggtgata
    10081 acgcggttcc caccaatcaa acgggtcagc atgaagagac tcaaacttgg gataaagacc
    10141 gactgaaccc cacttcgcac accgttcata gatagaggga agatgacatg acggaaggtc
    10201 tcccacttag ttgcaccaag gtcgtaacta gcattgatga gattattatc catatcgtct
    10261 aagacattga aaatcggcaa aatcataaag gggagctcga tgtagcttgc gacaaagata
    10321 aaggaaaaat cggtaaaaag caactgttgt gaaccaattc cgataaattc caagaattgg
    10381 ttaatagagc cattttgacc aaaaatcccg ataaaagcat aggctttaag gagcaaattg
    10441 atccaggtag gaaggataat cagcatgagc cagagttgac ggtgcttgag acgggtcaaa
    10501 aagagggctg ttggatagct gataagcaag gtcacaaagg tcacaatccc tgcataaagc
    10561 actgagttga aactcatttt gaggtaggtc aagttttgtg acgcaaagta agatttataa
    10621 ttttctaaac taaactggcc ttcaatgttg aaaaaggatt gtccgaaaat caagaccaag
    10681 ggtgccaata caaagagggc aatccaaagc atgtagggca ctacaaagag tttagagctt
    10741 gttttcttca tctctttcct cctcgatcgc attgatcaga cctgcttctt gctcttcgat
    10801 ttccacgtat tcttcgatac gagcatcgaa ctcttcttcg gtttcattga gacgcatgat
    10861 gtggatgtct tctgattcaa agtccagacc gatttcctca cccacgatgg ccttacgggt
    10921 tgagtggatc atccattcat ttccaagttc gtcataggcg ataatttcat aatgaactcc
    10981 acggaagagc tgggtgtcga ccttaacttg gagtttgcct tcttcaggaa gggtaatgcg
    11041 caagtcctct ggacgaataa cgacctcaac aggttcattt ggcttcatcc caccatcaac
    11101 cgcttcaaag cgtttgccgt taaattcgac caagtagtcc tcaatcatgg tacctggcaa
    11161 gatgtttgac tccccgataa aggtggcaac aaagtggttg attggctcat cgtagatgtc
    11221 cacaggggtt ccagactgga caatctcgcc atcattcata acgaaaatcc agtcactcat
    11281 ggcaagagct tcttcctgat cgtgagtgac aaagacaaag gtaatgccca atcgttgttg
    11341 taattcacgc aattcgtact gcatgtctgt tctcaatttc aagtccagcg ctgataaagg
    11401 ctcgtccaac aagaccacac ggggttggtt gatgatagca cgggcgatgg ccacacgctg
    11461 acgttgtcct ccagaaagtt tgcggatgga acgtttttca taaccttcca actgaaccat
    11521 cttgagaact tccgctacac gctgctcgat ttctttctta tcaattttac gcaagcgaag
    11581 tggaaaggca acattttcaa acacattcat atgtgggaac aaggcatagg attggaagac
    11641 ggtatgtacg tcgcgcttgt tggttggaat atcattgata cgaacaccgt ctagcatgat
    11701 atctcctgtc gtcgcatcca gtaaacctgc aataatgttt aggatagttg atttccccga
    11761 accagatgca cctagaaggg tgtagaattt cccttcttcc aactcaaagt tgatgtcttt
    11821 gagaaccttg gtgttgctgt cttcaaaaac tttagagacg tttttgaatt cgataattgg
    11881 ttttttcaat tggcataaat tccttctttt tcatagatta accgatcggg gctctgtcag
    11941 gtccccacta cctcttgcag ggagtaaaac cacctgcata catcttcgct accgataggc
    12001 tttcaccccc tttccatgac atagcagcag cttttcaaat acagcttcct acttgctttc
    12061 acccaagatc cggacttctc tttcaagcgt aatacctgag tgttccttga ctttttcgat
    12121 aaccgattgg atcaagtcct cgtagtcttt ggccgttcca tctgcgacat tgatcataaa
    12181 tcctgcatgc ttttctgaca cttctacgcc accgatacga tagcctttca agccagcttc
    12241 tgaaattaac tgacctgcaa aatgcccgac tggacgctta aagaccgagc cacaagatgg
    12301 gtattccaaa ggttgcttga gttcacgtag gtgcgtcaag cggtccattt cctgcttgat
    12361 aacctgatgg gttcctgggg ctagggcaaa tttaactgac aagacaactg caccagactc
    12421 ctgaatagct gaatggcggt aaccaaaagc caagtcttta gcagacaggg tttcgatttc
    12481 tccatccttg gtcaagacct tacaagactg caagatgtga gcaatctcgc caccataggc
    12541 acccgcattc ataaagacag caccgccaac gcttcctgga ataccacaag caaactcaaa
    12601 gccagtcaaa ctatgacgga gggcaatgcg agttgtttca atcaagttag ccccagcttc
    12661 tgcttcaatg gtatagccat caacagaaac gttattgagc ttgtcacaca agatgacaaa
    12721 tccacgaatc ccaccatcac gaacgatgat attgcttgca ttgccaagaa ccatccaagg
    12781 gatattttct tggttggcaa atttcacaag gcgcgccaac tcaaaacgat ttcgtggaaa
    12841 gaccaaataa tcagcctctc cacctacttt tgtataacta tagctatgca agggttcctt
    12901 aaaacggata tcaattcctt ctaagatttc aagcattttt tctcttacag acatgtcact
    12961 cttcctttta caaaattcat tccattatac catttttaga gacatttgac gaccataaaa
    13021 ataccttgtt tggattttgc ataagaaaaa gaggttcccc cctttttatg attttttaca
    13081 aaagatttcc ttggttccat aggcgaccag aacgagctcc agtgctagaa tcacttcaac
    13141 caagactgga tttgtcaacc agcctacttg gaaaagagat ggtgccagat caaagaaggc
    13201 atgcaagcca taggctgcta ggagataaat ccatttcttc tggcgaacag cttggtaaac
    13261 ccaaactgtc aaaagtaatt ggaaaccaag cgccaagatt cgctcaaaac caagcaaata
    13321 aatctgccag accgaaagtg actgaatggt ttttaacata ttttcagaca gtaattgcat
    13381 aacctgtgga ttctgagttt gaactgccga aagaacgatg taaagattga gtaaactaat
    13441 aaggcctaga aaaatcaact ccaagccacc atgccctagg ccataggcca aggcatctgc
    13501 cttttccaaa cttctctttt tctccaacca tttgaagaaa acaagacgag ccgtttcctc
    13561 aaaaaatgct gccatggcta ggccatagat aatatagata agcggatgat cttgcaagag
    13621 ggcaatacta ccgtcttttt gagggtgcaa aatcaagata tgcaccagtt tttccaaaat
    13681 ctgcgaagag acaaagaaag cgactgcacc caagcccaag acagctagat taatctggta
    13741 tttctttttg gcaaaccaaa tgcttcctat cagaaaagct agcaacagca acatggtaat
    13801 aatgatatga atggtcattt tcttctccta ttctgccttt tcaatatctt ttttcatctc
    13861 gtcaacattg aacttagcaa acaagtattg acggtcttgg actgggaaac gttggtccaa
    13921 ctggtcaact gctcccacct cgataatgcc ttccttgatg acaaaatcca tgacatgtcc
    13981 accgaaggtc aaatcatctg atatgaagtg cagatggtag cctgccacac tgaccccatg
    14041 gaaaatctca ggcgtccaga aaccaacaat agtccccgca acattgtcac gactatattc
    14101 cggttgatgg gttgcgacat cagcaaactt agtatcgggt gttgacttag gaatcatacg
    14161 cacatgcata tgtgaaaatt ctccccgaat cttgatagaa cggaaaagat tttccccatc
    14221 ataataagac tcaattcgtt ctttcaattc cttgtctgtc atctcaaagc gctggcggaa
    14281 aatgacctct gcctgatgcg gtaccactgc agcgtaagga ataagggcat ctggtgacac
    14341 ttccacaatt tctggctggt ctcctgaccc tttggcctga taagccttgc catccaagac
    14401 aatcaattcc ccatcaatag aatccaaggt tcccaaacca aggtcaccat gctctagcaa
    14461 ttctcccact gtcatggtac caccataaag gccagccatc aaggctccaa gagtattgta
    14521 ttgaaataat ttcactggtt cctgcacgtt cttatccatt cactttcttg tctgttattc
    14581 tataaatctt actcctaagt ataccacatt tgcccctaga tgtgaacgag agaaacgctc
    14641 tagacattgc caagaaggaa aaaaaagggt acaatgtaac aaaatcaagg gaggtctgga
    14701 atgaagaaac aaagcaagta caaagaggtc gtttcctatc tgaaaaatgg tatcgagtct
    14761 ggacgatttc cgacgggtag tcgcctgcct tctatccgtc aactgagcct tgactttcac
    14821 tgtagcaagg acaccattca acgagccctg ctggaattac ggcacgaaca atacctctat
    14881 gccaagcccc agagtggcta ctatgtccta gaacaagggc aacaccagga cctagaaatc
    14941 gaggttaccg acgaacatgc cagtgcctat gacgatttcc gactctgtgt caatgaaacc
    15001 ttgattggcc gagaaaacta cctcttcaac tactatgata atcaagaagg attagaagac
    15061 ctaagacaat ccattcacaa actcctcttt gagcaagctc tctactgcaa ggctaaccaa
    15121 ctagtactga cttctggaac ccaacaagcc ttgtttatcc tctctcaaat atcctttcct
    15181 agccaagcca aggaaatctt ggtggaacag ccaacctacc atcggatgaa tcgcctcttg
    15241 attgcacagg ggctggacta tcaaacgatt gaacgaggca ttgatgggat tgacctgaag
    15301 gagctggagc gacacttcaa gacaggaaaa attaagtttt tctacaccat tccccgattt
    15361 cactatcctc tgggacattc ctattctgag caagacaaac gatccattct taacttagct
    15421 gccaagtatg atgtctatat cgtagaagac gattatctgg gtgatttgga ctccaagaag
    15481 ggccaaacct tccactatct tgatacagag gagcatgtca tttatatcaa gtccttctcg
    15541 accagccttt ttcctgccct tcgtattaca gcactcattc ttccaaatgc tatcaaagaa
    15601 gcatttgtgg cctacaaaaa tatcctagac tacgacagca acctcattat gcaaaaggcc
    15661 ctgtcactct atattgacag tcaattgttt gaaaaaaatc gtttggctcg cttgaccaat
    15721 catgaatctt accaaaaaca aatcgaggaa aggataacta aaacaccttg tccccttcct
    15781 cattatcccc tacacgatgg tttattgcta gacctgagac agtatcctaa aatcgccagt
    15841 ctcaaacaca gtcaaccggg cttggacttc tttgaagagg cctatttaag tacctgtcct
    15901 tatcaatttg ccaaggtgtc cctagacaat ctggaaaatg ttttaaacta tttaaaagca
    15961 gaattggaat gacatccaac tctgcttttt cttattcttc aacttcagtt agacttgctg
    16021 ctttttcaag atagctttgg taaattcctt catccttaag tttttggatg accttgtcta
    16081 ccacttcttt caaatcatca ctattttttc taagagcaac ggcattggcg tcgccgtcct
    16141 tcatcttcaa gctgacagtt gcgacagcta agccagcgtt tttagcagca taactaagtg
    16201 caacaggctc atccatatga acagcatcta cttttccagc ctgcaattca ttgactgctt
    16261 cacccatatt agttagggaa gttaattgag cttttggcaa ttgttccttg accattgatt
    16321 ctggaacagt ccctttttgg gctgcaatat tagcactttc taggctagtt aaatccttgt
    16381 atttttccac atcagcctta cgaaccaaga aactaatctt gttttcatag tatgggattg
    16441 aaaaatcaaa gacttctttt ctctcgtcag tagcactaat tcctgcaact gctaggtcag
    16501 ccttaccagt ttgaagactg gtcaaaacat tgtcaaaact catgcttgag atttccaact
    16561 taaccccaag ttcatcagcg atagcctgag ccatgtcgat gtctgcaccg actacctggt
    16621 tctttccatc aaccaatgat tgaaattcaa agggtgcata gtcaggactg gttgccacaa
    16681 ctaatttccc cttttgctta atggcctcaa cagctgactg agaaccatta gaacctgact
    16741 ggcaagctac taagaagaaa cttgcaagaa aattacataa aacaaatatc catttttttg
    16801 atttcataaa taaacaacct ctctgttaat tttgtataat tataacgata tccaagttac
    16861 ttgtcaagtg ttttttaaat ttttatctca aaaatatttt ttcgttcaaa aaaaagagcc
    16921 atcagttgat ttcaagctcc tttttataca gaattaaact attttatagt tcgacaatct
    16981 tacctgtttc aaagtagaca acccattcac agatattttt agcatagtca ccgatacgct
    17041 ccaagtagga aataacttgg aaataatcac gacccgtaac aatggcttct ggatttttct
    17101 taatctcttc agtcgcaagg tcacggatag tttcaaaata gtggttaatt tgctcatcca
    17161 tggaggccac ccggtatgcg tcgtcaacag aaccattaag ataaagatca agtgctgctt
    17221 caacaacgct tttaacttca cgtcccattt ttttaatttc ttcctctaca gctggaatgc
    17281 gctcttcccc cttcatacgg atggttgcct gggcaatggc tacagcgtga tcccccatac
    17341 gctccacatc tgatacagcc ttaaggacag tcaagactgt acgcaaatct tgagagactg
    17401 gttgttggag tgcgatcatt tcaaatgatt tcttttccag tttcacttcg tattcattta
    17461 cttctgcatc atcttcgatg acctcttttg ccaggtcacg gtcatgcgtg acaaaagcac
    17521 gtaccgtacg attgatttgt gagagcactt cttgtcccat agcgtagaac tggttatgta
    17581 atttctctaa atcttcttca aattgagatc gtaacatctt tcatctcctt atccaaattt
    17641 tcctgtaata tagtcttccg tttccttgtg ttggggatca aggaacatct gcttggtatc
    17701 attaaattca atcaaatctc catctaggaa aaatcctgtc ttatcagaga tacgtgaagc
    17761 ttgctgcatg gaacgggtta ccagaagcat ggtgtacttg tcttttagac catacaaggt
    17821 ttcctcaatt ttaccagctg aaatcggatc caaagccgaa gttggctcat ccaagaggat
    17881 gattttagga ctagttgcca agacacgggc cacgcagaca cgctgctgtt gaccacctga
    17941 caatccaata gctgaatcat atagacgatc cttgacctca tcccagatag aggcaccttg
    18001 caaggctttt tctacggctt catccagaac ctgcttatcc ttaattccat tgatacgaag
    18061 cccgtagaca acattctcat agatagtcat agggaaagga ttaggttgtt ggaaaaccat
    18121 tccgatttcc ttacgtaatt caaccgtatc tgtacgcgga ctgtagatgt tgtggccatt
    18181 gtacaccacg gatccagttg tggtcacctc tggattgaga tctcccatgc ggttgagaga
    18241 cttgaggagg gttgacttcc ctgatccaga tggaccaatc aaggctgtaa tttccttagg
    18301 ttggaaagat agggaaacac tattcaaagc cttcttttta ttataataaa cggacaggtc
    18361 tgatacctgt aaaatcgcat ctgtcatacg gtttcctttc taaccaaagt gaccagatac
    18421 atagtcattg gtggactgta gcttggcatt ttggaaaata gttgcagtct tgtcatactc
    18481 aatcaaatca cccaagtaaa agaagcctgt gtagtcactt gcacgagcag cctgctgcat
    18541 attatgcgtt acaatgatga tggtaaagtt tttcttgagc tcaaacatgg tctcttctag
    18601 ttgcatggtc gcaatcggat ccaaggctga ggctggctca tccattaaga ggatatctgg
    18661 cttaacagag atggcacgag cgatacagag acgttgttgc tgaccacctg ataaggtcaa
    18721 ggctgacttg tggagatcgt ctttaacctg atcccagagg gcagcctgac gaagggaggt
    18781 ttctacgatt tcatctagga cttgcttatc cttaactcca gcacgttcat gcgcaaaggt
    18841 aatattacgg taaattgact tagcaaatgg attggggcgt tgaaaaacca ttccaatgtg
    18901 tttacgcatt tcataaacgt tgatttctgg acggttgaca tcaattccac gatagagaat
    18961 ctgcccagtt actttagcaa tatcaatggt atcattcatg cgattgagac tgcgtaagta
    19021 ggtagatttc cccgatcccg acggaccaat caaagctgta attttatttc tttcaaattg
    19081 catatcaatc cccttaatgg attcattttt accatagtaa acatggacat ccttagtaga
    19141 aagggctact ttttcttcag gaaaggtaag gatatgcttc tcatcccagt tatatgttga
    19201 catggcttct cctttaggca gcggttaatt tcttgtgtag atagcttccg aacttacgag
    19261 ctccaaagtt aaaaatcagg ataaagatca ggagcacagc ggcagaacct gctgatacaa
    19321 tggttccatc tggaatagtg ccttcactat tgactttcca gatatggaca gccaaggttt
    19381 ctgcttgacg gaagatagag atggggctag tcacactgag gatattccag ttagaccagt
    19441 caagagctgg cgccgattgc cctgctgtat agatcagagc tgcagcttcg ccaaagatac
    19501 gaccagatgc caagacgaca cccgttacaa tacctggaag cgcttctgga ataacaacat
    19561 gaaccactgt ctcccagcga gaaatcccaa gagccagacc agcctcacgt tgggtatggt
    19621 gaacgtgttt caaactatcc tctacattac gcgtcatctg aggcaagtta aagactgtca
    19681 aggccaaggc acctgaaatg attgaaaacc catactcaaa ctggactaca aagatcaagt
    19741 aaccaaagag acccaccacc actgatggta aagaggacaa gatttcaata caagtccgca
    19801 caaagttggt aacaggacct tttttagcat attcagccaa gtaaatccca gctcccatag
    19861 aaagaggtac agaaataatc aaggtaatga ccaataggaa aaaggaattg taaagctgaa
    19921 tgccaatccc accacctgct tgaaaagcag aagaccttcc agtcaagaaa gaccaagaga
    19981 tatggggcaa gccccgaacc aagatataga gaattaagga agccaagatt gtcacaatga
    20041 tgctagcaat cgtatagagg acagctgttg caagtttatc taatttctta gcgcgcataa
    20101 tttttctttc ctctttcttt cgtaatcaat ttaatcacac tgttaaaaac taaactcatc
    20161 aagagcagta ccaaggccag tgaccagaga acattattat ttacagttcc catgacagtg
    20221 ttcccaattc ccatagttaa tatagaagtt aaagttgcag ctggtgtggt caaggaagtt
    20281 gggataacag ctgagtttcc gacaaccatc tggatagcta aagcctcacc aaaggcacgc
    20341 gccatcccaa agaccactgc agtgaaaata ccagaacggg ccgccttcaa gatcacacgc
    20401 cagatagtct gccagcgagt ggctcccata gcgaaactgg cctcacgata ataacgagga
    20461 accgcacgca agctatccgt tgtcataaag gttacggtcg gcaaaatcat gacaaagagg
    20521 acggaaatcc ctgacaaaat cccaaaacca gtcccaccaa agacactgcg aacaaaggga
    20581 acgacgactt gcaagccaat aaatccgtac actactgaag gaatcccaac caggagttca
    20641 atagctggtt gcaaaatctt cgcccctttt ggtgatactt cggtcataaa aactgctgca
    20701 ccaatagcaa agggtgttgc gataagggct gagagaatgg taacgataaa ggaacccaaa
    20761 atcataggaa gggcaccaaa ttctttacta gaaggattcc aagttcctcc caaaagaaag
    20821 tcaaagatat tcacaccatt gacaaagaag gtcgacaagc ctttttgcgc tacgaaaacc
    20881 aaaatcatgg ccacaaggat gactatcaaa gaaagacagg caaaggtcaa accttttcct
    20941 aatttctcca gacgagaatt ctttgatgga agcaacattt tcttagctaa ttcttcttga
    21001 ttcattattg tctcccttcc aacactgtca cagttccggc agcatctttt tcaaccttca
    21061 tttccttaat cggaatatac ttcaatcctt tgacaatccc ttcttgggtc tcatccgaga
    21121 gaacaaaatt gagaaattct gcagccaact cattgggctg ccccaatgta tacatatgct
    21181 cataagacca caagggccaa ttattgctac ttatattttc tggacttaag tcatagccat
    21241 tcaacttcat gcttttgacc gaatcatcta tataggtaag agataaataa gagatagctc
    21301 ctggactttt tgatacgatt gattttaccg ctccatttga atcctgctcc tgactttgca
    21361 tggcagactg accttccata atgacagtat caaaggtagc acgagaacca gagccggctg
    21421 cccgattgat aacagagatg ggtaagtcct taccaccaac ctctttccaa ttggttacct
    21481 cacctatgaa gatttgacga agttgctctg tcgttaggtt atcaacatca acctccttat
    21541 tgacaatcag agccaagcca gctaccgcga ccttgtggtc aacaagagca gaagcatcaa
    21601 ttccgtcttt ttcctcagca aatacatctg agtttcctat atcaactgcc ccagactgaa
    21661 cctgggacaa gcctgtacca gaacctcccc cttggacatt gaccgttttt ccaacatgga
    21721 tcgtgccaaa ttcatctgcc gctacttcaa ccaagggttg caaggcagtt gagccaacag
    21781 ccgttatgga ttctccacga tcaatccagc tagcacagcc tactaaacaa gccgtcagcc
    21841 aaaaagcgat aagagacaga gcaagctttt ttcttttttt cactgttttt ctcctcgaaa
    21901 ataattatga atactgtgaa ttttttaagt agttctttat gagttgacgc atgaattctt
    21961 accaaatttc tgcgcaattg attatttata taatataggc tatattactc tttcctaacc
    22021 tccttttttc atatgtggat aaaatctctt gtctatccct tcccccattg tcacccatta
    22081 tagtcatttc gtgtctcttt ttcccctttt taatgcaagg gaaattactc tccttagatg
    22141 ataatccaaa agctagaaag gtatctcaaa cctctctact ctcccagact agtttacaac
    22201 taaaaggaaa agattctatt ttatgagaaa tctagtttac aagcggtaag aacgctaata
    22261 actaaacttc ttgtactctt tgaaaatctc ttcaaaccag tgttttgagc tatctatggc
    22321 tagcttccta gtttgctctt tgattttcat tgagtagtaa aactacatgt aatggcaatc
    22381 aagatatcaa gaatcatcct actaaaaaaa tccatacttt cactataaca tagaataaga
    22441 tatttgacta gcattttcat ttgaatctga ggccttttgg aaaataattt ttcaaaacat
    22501 ttccagtaac ctttgcaaag cccaagccat tgcctttaac caaaacttgg taccaaccat
    22561 ttggcagact ttctgccagc tgaacgattt ctccagccgc atacttgaca aacgcttctt
    22621 ggccaatttc aaccgactgt tcgacctgac tcggtttcaa ggctaaacca agagcgaaac
    22681 tgggctcaaa gcgtttcttc ttaaaagtgc ccagatgcag tccattgcga gcaatcttga
    22741 gcttccctaa atctggcaaa agttctggca agagataaag ctggtctcca aaagtctgca
    22801 agatacccgg tagattgacc ttcaaatggt tttgggcaaa ttcctgccac aaggcaactt
    22861 gttcacggct gaggttactc ttacttgcct taaatttagg agctggattg ttacccttaa
    22921 actgtagatg ggcaacaaac tgaccctctc ccttaaactg atgagggtac atccgagccg
    22981 tttctggcag gtcaatacca gctaccattc cattgatatg ctctactggc aacaagtcaa
    23041 aatcatactc ttccagtaac caattgacaa tctcttcgtt ttcctcgggt gcccaggtac
    23101 aggtcgaata aaccagacga ccaccttcag ctaacatggt cactgcatcc tccagaattt
    23161 ctctttgcaa gctagcacat tgactcggat aatctaagct ccaatagtcc atagcatcag
    23221 gttgcttacg aaacattcct tcaccagagc aaggggcatc aagaacgatt aagtcaaaat
    23281 agcctttaaa gaccttgacc aagcggtcgg cagattcatt ggtcaccacg acatttgtcg
    23341 ctccaaaacg ctccatgttt tcaaccaaaa tcttagcccg tttgcttgaa atttcattgg
    23401 aaacaagtag cccctcccct gctagatagg ctgccagttg agttgatttg ccccccggtg
    23461 cagcagccaa gtccaagacc ttcataccag gactgggttg agctacttga gccaccattt
    23521 gagcagcagg ttcttgcgaa taaactaaac ctgtagcatg ctcaggcgat ttccctgaaa
    23581 ccttcccata gtggccccaa ggggtttgag taatggcatc agaaaaggaa agttgctctt
    23641 cttttaaggg attgacccga aaggccgaaa ccgcttcctc ctcaaaagag gcaagaaaat
    23701 ctcttgcctc atctcctagt atctctttat atttttcaac aaatccttct ggaaattgca
    23761 tttaagttct tttcctttcg taaatatagg actgaatttc ctcctgcatc tcaagaggca
    23821 ccatcatgac cggctgtctg gtttgaaaat caggagcttc accaaaaagg gtcacaaccc
    23881 gatagcccag actttcccct aaaatactag ctgcggcata atcccatggt tgcagataag
    23941 tgagataggt caacaaacgc cctgacaaaa tcttggcaaa actaatggcc gcacttccat
    24001 agacacgaac accaagaacc gctcggctca aatcagccag cccccattca ttggtttcca
    24061 gcataccact attccctgca atgagaaaat ctccaagtgg tttagtttta aaaggagcta
    24121 ggggcctatc atttagacaa actggaaatt ccccaccacc gtggtaacaa tcccctttga
    24181 ccacatcata aatcagacca aactgtccct gaccattttc aaaataagcc atcataacag
    24241 caaaatcttc ctgctgggct acaaaattat tggtaccatc aatgggatca atgacccaaa
    24301 ccttgccctc ttgaaccgag gctcgcagac aaccttcttc agcacaaatc ttatcctcag
    24361 gataacggga caaaatctca ccaaccaaga gttcctgaac ttctttgtcc agtctggtca
    24421 ccaaatctgt tggagaggac ttggtttcaa cacgcaagtc ttcctgcata tggtcaagaa
    24481 tgtactgacc tgctttctta acaagctctt tagcaaattc aaatttactt tccaagagaa
    24541 atctttcctt cccctttttc tttggcagcc tgaactgctc ggtaaagtga atagccacta
    24601 accgtttcaa attctcgtcc caaacgtttc tcttcgctct tgcttggcac gaccgctttg
    24661 aatcccttat aggagtccag taacttttta gcctctactt tgccttcata ggcagcttca
    24721 acatcattaa aaaaagaaag cactgaagca agttcttcag tgctccacga caaatctagt
    24781 gggtaactat actgtttgtt cattaactaa taccagctct cattcttgct tcttttagtt
    24841 cttgcttacg ataactacga gggagaaaag cacgaatctc atcttcatta aaaccgattt
    24901 gcatacgctt ggcatcaata ataattggac gacgcaaaag actaggatac tgctcaatca
    24961 aatgaagcaa ttccgatacc gaaatactct ctacatcaat attcaatttt tgaaaaattt
    25021 ttgaacgagt tgaaatgatg tcatcagtac cattttcggt caaggaaagg atgtgttgca
    25081 attcttttct tgttaaagga ctggtcataa tattgtgttc cacaaaggga accttatgtt
    25141 tttctaacca ggccttagcc ttacgacatg atgtacagct cggtgataga aatagtgtaa
    25201 tcatgctttt ctcttcttat ctatactttg ctacttctat tatacaaaaa aataaggcgc
    25261 ttgactaggg atttttagaa aaaaagccta ttttttcaag aaaaataggc tgtttgcgaa
    25321 cgattgacac aattggattt ggttaattca ctcttaacga tggttttaaa cgatatatat
    25381 ttttatatat gcaaattaaa aacatctttc atttcacttc ctacgacttt tcagacacag
    25441 atagccaaag aagctttcat agagggcaaa aaagaggagg aaggcatgaa gaaagaaggt
    25501 ctctggcaaa atcataataa caggatcctt ggctggatca aaaagccagg tatcatctcc
    25561 cacaaagaga atttgatgga aaagagtaaa gaattggtca aaaccaatca aaactccccc
    25621 aagtccaatc atcacaggta agactactag agccaggaga ctttttcgat aaagagacaa
    25681 aaagtccttt ttcacaatcc tattgacaaa gacatagaaa cttggcagtg tcactagagc
    25741 tactagctga accaaatgaa agagattctt gaccactgcg aaatggtgca gaccagctgc
    25801 tgacgaacga aaatcaggca tctgtaagac ctgactaaaa ggattggtca gataattcat
    25861 caagatatga aaattgtatt gaatggtttc tggttttaga tagactcgat tcgttaagtt
    25921 tagccactga atctccatag gatagaaaat ccaagccaga taaatggtca gaaggattga
    25981 gagggagagg agaaagagca tagagcccca aaaggtcaat ttagttttca tcaaaatccc
    26041 actccgcaag gctagaaacc acatgtgtcg gtgcgattgg caggccagct acttcttctg
    26101 ccttggtaaa acctgtcgtc accaagagcg ttggaatgcc attgtcaatc ccagcccgga
    26161 tatcagtcaa ataattgtcc ccaaccatga ttaactcttc acgttccaaa cctaagtgct
    26221 caaccgcctt gtccataatg atggcatttg gttttccgat ataaaccggc ttcactcgtg
    26281 tcgctacttc aagcagcgta atcagtgagc cagcacctgg caaaagaccg cgttccgtcg
    26341 ggatgttgag gtcaggattg gttccgataa aatgggcacc cttttgaata gcaagagttg
    26401 ctgtggcaaa tttttcatag tcgacttgcc aatccagacc aactaccacg taggcaggtt
    26461 tttccttgtc ttccacataa ccagccgcct tgatggcttc cttgagtcct gcttctccga
    26521 cgacatagac ggtcttttca agccccaaat cattcatata gtcgatggtt gccaaagtcg
    26581 ctgtgtagat agtcgatagg ggcgtatcga tattaaaatt ctgagccaac atctccttaa
    26641 cactctctgg agtgcgggtt gtattgttgg ttacaaagag atagggaatg tcccgctttt
    26701 gcaattcatg aacaaaagtc tctccagcag ggattcggtc tttcccctta taaatggttc
    26761 cgtctaaatc aattaaatag cctttatatt tcatctattt ctccctaagc cttttttatt
    26821 tcttgccaag taatgattgc ttgggcattg ataaccccat cacttgtaat ttcatgcttg
    26881 ctttccagtc cagtccgttc aacagccgat gtaatcaccc cacctggtcg aacttccttg
    26941 acatacttga ggttgatttt cttgggaata tagtgggtca aaaaatccgc tcccatgacc
    27001 tcaaaaatcc agtccaagta tttactgtta ttgacatgac cattcatatc caagtcgtaa
    27061 aaacgaacat ggtaatcctt gctgatcggt tcttccaagg actcatactt cggtccacgg
    27121 ataagttttt tatcaaaatc agactggtaa ggagccacaa tctcaggttc aacaacatgg
    27181 acttttcgac tgtcgcggtc catgagaaca aaggtcgcca tcatgtggat gagctcctgc
    27241 tccgcttcat tataaatagt aaagcgacgg tagcaaaaaa gtcgattgta gctcaaggct
    27301 tccgtttcga tggtaatttc ttccgcaaaa cgaggcaaac gaaccacctc aatatcatat
    27361 tctacgataa tccagaccag attatattct tccaaaatgg ccttatcact aactcccagt
    27421 tcaatcgact gcatccctga aacttgcagt gacagcaaaa tcacatctgg aagtttgata
    27481 tgaccgttca tatcagccat atcaaaagga attttcattt tcatttgata agttaagccc
    27541 atgatcctac tccaaaataa atcgttctgc tacagtatct cccaaaaaga gacctctctt
    27601 tgtcatgcga acgtggtcac cctcaatctg catgaggcct tgttgaacca aatctctgac
    27661 aatttctcca taaagtccag caaaagactg tccaaatttt tcctcaaatc gcgccatgga
    27721 aaccccggat ttcttgcgga gtcccaagaa catttcttct tccatttgct ccttttgact
    27781 caggtgatct tctgtaatac aagcattgcc ttcctcaacc gcactgagat aatgacgaat
    27841 gggaccatga tttttatagc gtactccatt gacataacca gatgcccctg caccaatacc
    27901 atagtattca gcattgtccc agtacatgag attgtgacga ctttcaaaac cgggtttgga
    27961 gaaattagaa atctcataat gctcaaaacc cgctcgctcc agctctgcaa tgatgtactc
    28021 aaacatctcc gcttctagtt cctccttagg cagaggcaat ttcccacgtc gcatccggtt
    28081 cataaagacc gtatggtttt ctaaaatcaa actatacaaa ctcatgtggg gaatatccag
    28141 tccaatggct ttagccacat tttcctttac ttgctccatg gtctgaccag gcagagcata
    28201 aatcaaatca atggagatgt tgtcaaaacc agccagtttc agacgatcga tattttcata
    28261 aatatccttc tccaaatgac tgcgcccaat ctttttcaac atcttatcat caaaggtctg
    28321 gacacctagc gaaacacgat tgacagccga atttttcaaa acagctatct tatccgcatc
    28381 caaatcgcct ggattggctt caatggtcaa ctcttccaag acagacaagt ccaagttttt
    28441 agtcaagcca ttcagtaaca cctccagttg cggagccgac agggctgtcg gtgttccacc
    28501 accgatataa agggttgaca acttttcaat atcataagaa cgaaactctt ccagcagatg
    28561 ctctaaatag ctgtcgactg gctgattttt gatgaagacc tttgaaaaat cacaataata
    28621 acaaatctgg gtacaaaatg ggatgtgcac ataggctgac gttggttttt tctgcatagt
    28681 aattattata ccacaaagac tagattccag ataaaaatca ccatccccag atacatagtc
    28741 cgtccggaga tggtgatggt ttattcttct gttatatcaa tcacaatctc ttctgagtca
    28801 tcaagagctt cggctttttc ttgccattgt tccttgagat tatttaattg attttttgat
    28861 gcttctgtcg cttgaaaagc ataggattta gcttgagcaa gtatactgtc cacagtgatt
    28921 tcacctgact caacctgttc ttttgttttc agaacaaaat ctgtagcctg ctccttaact
    28981 tctgtcagtt tttcacagac ttgctccttg gcatactccg gatcttctct caaatcatct
    29041 agaaaatctt gagcctgact gcaaacttgt ttgcccttat cacttgttaa aaacaaggca
    29101 agagctgcac ctgaaaccgt tcctaaaagg attgaggata atttacccat aaggattctc
    29161 cttttttatt ttttgaaaaa tttacttgca agacgaagag ctgacagact tgcaccagtc
    29221 ttgagtgttt ttgaaccagc tgatgaagct ttcttgctca agacacgcgc atggtcattg
    29281 aggtctgaaa cagatagaga taaatctgca acagcgctga agagtggatc aatcgtagcc
    29341 accttgacat tgatatcatc tgccaagaca ttgaccttag ccaacaactc attggtgtga
    29401 tgcaaggtca catccacatc tgaagtcaag gttttaatcg tcttttctgt ttcatcgatg
    29461 acacgaccaa gcttttgtac agtaatgatc agatagacca aaaagacaat caaagctagg
    29521 gcaacaagaa tatatgcaac ttctaacatt tatttttcct cctctgtaat atagtaaggg
    29581 gccttctttc gattttgata aataacgatc attataccga gaccgataag gacaactgac
    29641 agccattggg acactcgaag gctgaagaac atgagactat ctgttcgcat accttcgatg
    29701 accatacgac cgaaaccata ccaaatcaag taaaaggccg tgatatgacc tcgtctgaga
    29761 ctcttccatt tccgtctaaa aatcagaatc aaagcaaagc caagcagatt ccatagagac
    29821 tcataaagga aagtcggttg acggtagctc ccctcaatat acatctggtc acggataaag
    29881 ccaggtagat aatccagatt atccactgtt gcaccataag cttcttggtt aaagaaatta
    29941 ccccaacgcc ccaaactttg agcaatcata acgctaggcg ccgcaatatc tagaaaatcc
    30001 caagtattga tgagtttacg gtcagcaaag atatagagca caagagcccc agttatcaaa
    30061 ccaccgtaaa tggccaaacc accattccaa atggcaaaaa tctctcctaa attctgacta
    30121 tagtaatcaa atcggaaaat aacatagtag agacgagctc ctaaaatagc caagggaaag
    30181 gctactaaga taaaatctaa aatatcgtct ggtatgatct tctttctagg tgcttctttc
    30241 atggtcaaat aaaccgcaag aatcaagcct gtcacaatac ataaggcata ccaacgaatg
    30301 gctaggggtc ctagttgaat agcaattgga tcaagcattt tgcacctcat ttcgagcaat
    30361 gagacttgtc agtcgttcgt cgaacaaacg ggtcgcatca aagcccattt ccttggcacg
    30421 ataattcatg gcagctgcct caatcacaac agagatatta cgacctgttt taactggaat
    30481 acgaatacga ggaatggcta cgccagaaac ttcaagttcc tctgcattgt ttccaagacg
    30541 atcaaaggtc ttatgtgtat cgtaattttc caaatagaca gcaagctgaa cctgtgaaga
    30601 atccttgaca gcactcgcac cgtagagact cataacatcg ataataccaa ccccacgaat
    30661 ttcaatcaag tgtttcaaaa tttcagctgg ttcaccccag agagtaatct catccttggc
    30721 aaagatatcg acacggtcat cggctaccaa acggtgacca cgtttgacaa gctcaagacc
    30781 tgtctcgctc ttaccaattc cactatctcc ctgaatcaag acgcccatcc cataaatatc
    30841 catcaagaca ccgtgcacac tggtacgttc tgccaaacga gaatccagat agctagataa
    30901 ctctccagat aaacgactgg tagctgcacg gctggttaaa atagcaatct tacattctct
    30961 agcagccttt aacatctcct ctggaaccac caaaccacga gcaacaatga ctgctggtgt
    31021 ctcaggtaga aacatttttt tcaaaacttc ataacggctg ttggaaggca tgctgatcag
    31081 ataagaccac tccttcatcc ccaaaagttg aatccgctct ggtgtatagt agtcaaaata
    31141 gcctgtcatt tcaagaccag gtcgcgtaat atccgctgta ttgatttcct tttcaagcaa
    31201 ctctggttca ccatagacaa tgtctagtct aagcttttca atcacttctt ttactaaaac
    31261 cgacatcatt tcctccttca aatgagttct tctctctatt atatcaaatt gtaggaggaa
    31321 gtttcagatt tttgcaggct ttggagtatt tatttaaaag aaaaaggact agatttctct
    31381 agcctttcat ttcaattaga attttttacg tttacgagct acttctgatt tacgtttacg
    31441 ttttacagaa ggtttttcat agaattcacg tttgcgtgtt tcttgaagag taccagcttt
    31501 agtaaccgca cgtttgaaac ggcgaagtgc gtcgtcaaga gattcattct tacgtactac
    31561 tgttttagac atttttttca ctcccttcaa gttcaatatc tatttcattc tacacgtaaa
    31621 aggaataatt gtcaagcaaa aatgcggttt aattttgatt ttttcttcca tttatacaag
    31681 agttgaaagc gttcaataga acattgcttt tttatttttc gagtaagcta agcgcttcag
    31741 catctgcgat gatggttaca tcagggtgat tttgaaggct acttgcaggt aggttctcag
    31801 tcactgggcc agatactgtt ccggcaatgg cttctgcttt cgactcaccg taagcaaaaa
    31861 gaataataga cttggcatcc aaaatgtttt taatccccat tgaaatagct tgggttggga
    31921 cgtcttcaat cttggcaaag aagcgtgcat tggcttcgat agtagactgg tcaagttcta
    31981 ctagatgcgt ttgactgtca aatggagtac caggctcatt aaagccgata tgcccattgc
    32041 gaccgattcc caagatttgg aggtcaactg gatggtcagc caaaatttgg ttgtagcgtt
    32101 caacttcagc ttcagcattg tccttaacac cacgaggcaa gaaactttct ttaaatggtt
    32161 tttggttgaa caagttttct tgcatgaagt aacgataaga ctgtggattg tccccatcaa
    32221 ggcccacata ctcatcaagg ttgacactgg ttagatttga aaaatcaagg tcactctcaa
    32281 caatttcctt gtaaaattca agtgggctac ttcctgtcgc aagtcctaga gtttgagctc
    32341 cattagccaa tttttccttc aaaatctcaa aagcaacttt tccaccttga acttggtttt
    32401 caactttaat aactttcatc ttttcatcct ccatttctgt ctatatttat tatatggtat
    32461 agaccaattt ttgtcaagtg aaaacgattt aatttctaaa aaaagagcgt ccaaacttga
    32521 aacgtgttat aatggatagg attataactt agaaagaatg aacaaatata tgaatacagc
    32581 tgattttgat ttccacttgc cagaggaatt gattgcccaa acgccccttg aaaaacgtga
    32641 tgcctccaaa ctcctcatcg tcaaccgtga gacaggagaa atgcaagata aacatttcca
    32701 ctctattatt gatatgctgg aacctggtga tgcccttgtc atgaacgaca cccgagttct
    32761 ccctgcccgc ctctatggtc aaaaagtgga gacaggaggt catgtggaac ttctcctcct
    32821 taagaacact agtggagacg agtgggaagt tctggctaaa cctgccaaac gcctcaaggt
    32881 cggtactcgt atcagctttg gtgatggccg cctcagcgct gtcgttacag aagaattgac
    32941 ccacggggga cgcattgtcc gctttgaata ccaaggaatt ttcctagaag tcttggaaag
    33001 tctgggagaa atgcctctgc caccttatat ccacgaaaaa ttagatgacc gtgaacgtta
    33061 tcaaaccgtc tacgccaagg aaagcggctc tgctgcagca ccgactgctg gtcttcactt
    33121 caccaaagaa ctgctggcag aaatccaagc taagggtgtt catctagtct atctgactct
    33181 ccatgtcgga ctcggaacct ttagacctgt ttctgtggat aatctggacg aacacgaaat
    33241 gcactcagag ttctatcaac tttctgagga agctgctgcc acccttcgct ctgtcaaaga
    33301 aaatggtggt cgcgtcatcg ctgtcggaac cacttctatc cgcaccttgg aaactattgg
    33361 ttccaagttt gatgggcaaa tccaagcaga ttctggttgg accaatatct ttatcaaacc
    33421 tgggtatgag tggaaggtcg tggatgcctt ctcaaccaac ttccatctgc caaaatcaac
    33481 tctggtcatg ttggtttctg cctttgcagg ccgtgaatta gtcttagatg cctaccacca
    33541 tgccatccaa gaacactacc gcttcttcag ttttggtgac gccatgttta tttattgaga
    33601 aagaatttct ctaattcttc taataccaat aaatcgctaa gatattattt caaagaacat
    33661 ctacaattga aactctagct agctgtagaa gacgcctagt acattgaaat taaaatgctt
    33721 ccccctagct tcgaaaatat tgccatagat tgcgttgact ctccaaattg attcatctat
    33781 attttatttc agcttcctat attttcttcg ctgtttgtaa atcaaaatgc aagacacatg
    33841 agtagcacca tatttgttac tcttatctgt cctctcaaga gactattatg agttatttca
    33901 gaatcattca ctactttgac cctgactctc cttagtctca aaatcaaaga cttatactct
    33961 tcaaaaatct cttcaaaccg cgtcaacgtc accttggatt atatatgtga ctgacttcgt
    34021 cagttctatc tacaacctca aagcagtact ttgagcaacc tgcgactagt tttctagttt
    34081 gctctttgat tttcattgag tattaaacaa aaagtgaaca aatctgaatt ctaatgtaca
    34141 gaagactagg cttgttcact tttttatagt cgctataaga tgaccttatc tatagctttt
    34201 tatatataat tatatattca gacatactat tatcaatttt gtcgcaggga ggaatctgtt
    34261 aacgcaccca ttcaccatta tcattgactc tatagccatc tatacttgta ttgaccgcta
    34321 actcacccga tgcatttaca taataccatt taccaccaac ttggaaccat tgattgactt
    34381 tcatagaacc gttgctgttg aggtagtacc atgaaccatt aacttgtacc caacctgttg
    34441 ccatggaacc atcagtatta taaaaatacc acataccatt ttcttgtttc cagtctgttg
    34501 ttggagcaac tgctttagct ggttctactg ctacatctgt tccttggtta gatgtaacag
    34561 atacaggata cgaaggaata gatgattgct caggaacaac aactttttca ggttctctcg
    34621 tccctctcct tatacgtctt tttaccatct ctttagtaat ttgacgagaa gtagtttctt
    34681 caattgttcc atcacgttca tctacagtat agattgtagt aagagtaatt taccaatttc
    34741 tcctacttct tctacttctt gacttttatc aagagttggg ccatcgagat attctgtttc
    34801 gattggaatt tcttggacaa gaacttgggg cttggttctt tttttaacaa ctcttgtttg
    34861 agagtctttt tttgacttaa agtactctca gttacttgtc cactctttcc atctacatta
    34921 taagttatcg ttgtaactgt tttcccattc tttcctagag taatctcttg ctcctgtcct
    34981 gcagaaaggt cattgtctgc ttcatattta gtagcaaatg gaacaagaac ttcttcaacc
    35041 ttgcttttag ctggaacttt gataactgta tccgtggctt cttttctatc aacagtaacc
    35101 tgttcggtaa cataaccagt ctctggatta acatcgtagg tccttgtcgt agttacatag
    35161 ccatcctctc catcaattgt aacaggattt tcactacggt cttttgtttc atctttttca
    35221 taacgaattc gcgtacttga aattttcttg gttactacct taggtttagt cgctactttt
    35281 acaataatat ccccattgtc agcgtcatca tactctattc cctcttcttt atctctagta
    35341 tcatctctga catattgaat cccatcagca gcatgaacaa aacttgtatt cagattcctc
    35401 ctaaaaataa agttagcccg attaccgcag aaccaaaaat ctttccgagt ttacgtattg
    35461 catagcgctt attagtatta gattttgcca ttacatccta cttctagtat agcatctttt
    35521 ctatcaa
//
LOCUS       DAVMEI010000004        35326 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVMEI010000004 DAVMEI010000000
VERSION     DAVMEI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35326
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.004"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..409)
                     /locus_tag="VYQ24_000169"
                     /pseudo
     CDS             complement(1..409)
                     /locus_tag="VYQ24_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            404..658
                     /locus_tag="VYQ24_000170"
     CDS             404..658
                     /locus_tag="VYQ24_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1967362.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            953..2287
                     /gene="trmFO"
                     /locus_tag="VYQ24_000171"
     CDS             953..2287
                     /gene="trmFO"
                     /locus_tag="VYQ24_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1967363.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2371..3108
                     /locus_tag="VYQ24_000172"
     CDS             2371..3108
                     /locus_tag="VYQ24_000172"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1967364.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3117..3674
                     /gene="frr"
                     /locus_tag="VYQ24_000173"
     CDS             3117..3674
                     /gene="frr"
                     /locus_tag="VYQ24_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1967365.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3734..4588
                     /gene="cvfB"
                     /locus_tag="VYQ24_000174"
     CDS             3734..4588
                     /gene="cvfB"
                     /locus_tag="VYQ24_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1967366.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPVLKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4597..4812
                     /locus_tag="VYQ24_000175"
     CDS             4597..4812
                     /locus_tag="VYQ24_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1967367.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEESAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4898..5866
                     /locus_tag="VYQ24_000176"
     CDS             4898..5866
                     /locus_tag="VYQ24_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1967368.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5844..6023)
                     /locus_tag="VYQ24_000177"
     CDS             complement(5844..6023)
                     /locus_tag="VYQ24_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967369.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6059..6560
                     /locus_tag="VYQ24_000178"
                     /pseudo
     CDS             6059..6560
                     /locus_tag="VYQ24_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            6563..6822
                     /locus_tag="VYQ24_000179"
                     /pseudo
     CDS             6563..6822
                     /locus_tag="VYQ24_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7189..7515)
                     /locus_tag="VYQ24_000180"
     CDS             complement(7189..7515)
                     /locus_tag="VYQ24_000180"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967370.1"
                     /translation="MVDIAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7461..7874)
                     /locus_tag="VYQ24_000181"
     CDS             complement(7461..7874)
                     /locus_tag="VYQ24_000181"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV1967371.1"
                     /translation="MGAHFLTKQAGGSGVLLGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLMSNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(7875..8300)
                     /locus_tag="VYQ24_000182"
     CDS             complement(7875..8300)
                     /locus_tag="VYQ24_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1967372.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8477..9046
                     /locus_tag="VYQ24_000183"
     CDS             8477..9046
                     /locus_tag="VYQ24_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967373.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            9114..9764
                     /locus_tag="VYQ24_000184"
     CDS             9114..9764
                     /locus_tag="VYQ24_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1967374.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVKKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            9748..11988
                     /locus_tag="VYQ24_000185"
     CDS             9748..11988
                     /locus_tag="VYQ24_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1967375.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12167..12355
                     /locus_tag="VYQ24_000186"
     CDS             12167..12355
                     /locus_tag="VYQ24_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967376.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYARYEVINYYSGGY"
     gene            12388..12975
                     /locus_tag="VYQ24_000187"
     CDS             12388..12975
                     /locus_tag="VYQ24_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967377.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            12979..14163
                     /locus_tag="VYQ24_000188"
     CDS             12979..14163
                     /locus_tag="VYQ24_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967378.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIVLALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASPGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKEFFN
                     RF"
     misc_feature    14327..14458
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14474..15004
                     /gene="infC"
                     /locus_tag="VYQ24_000189"
     CDS             14474..15004
                     /gene="infC"
                     /locus_tag="VYQ24_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1967379.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15037..15237
                     /gene="rpmI"
                     /locus_tag="VYQ24_000190"
     CDS             15037..15237
                     /gene="rpmI"
                     /locus_tag="VYQ24_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1967380.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15289..15648
                     /gene="rplT"
                     /locus_tag="VYQ24_000191"
     CDS             15289..15648
                     /gene="rplT"
                     /locus_tag="VYQ24_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1967381.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15706..16086
                     /locus_tag="VYQ24_000192"
     CDS             15706..16086
                     /locus_tag="VYQ24_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1967382.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16336..17136
                     /locus_tag="VYQ24_000193"
     CDS             16336..17136
                     /locus_tag="VYQ24_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1967383.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            17099..18085
                     /locus_tag="VYQ24_000194"
     CDS             17099..18085
                     /locus_tag="VYQ24_000194"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1967384.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18127..20160
                     /locus_tag="VYQ24_000195"
     CDS             18127..20160
                     /locus_tag="VYQ24_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1967385.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMTSD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWFFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20221..21876)
                     /gene="pavA"
                     /locus_tag="VYQ24_000196"
     CDS             complement(20221..21876)
                     /gene="pavA"
                     /locus_tag="VYQ24_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1967386.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEM
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21981..22478
                     /gene="ybeY"
                     /locus_tag="VYQ24_000197"
     CDS             21981..22478
                     /gene="ybeY"
                     /locus_tag="VYQ24_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1967387.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22459..22854
                     /locus_tag="VYQ24_000198"
     CDS             22459..22854
                     /locus_tag="VYQ24_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1967388.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22871..23770
                     /gene="era"
                     /locus_tag="VYQ24_000199"
     CDS             22871..23770
                     /gene="era"
                     /locus_tag="VYQ24_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1967389.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23818..24642
                     /gene="mutM"
                     /locus_tag="VYQ24_000200"
     CDS             23818..24642
                     /gene="mutM"
                     /locus_tag="VYQ24_000200"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1967390.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24642..25247
                     /locus_tag="VYQ24_000201"
     CDS             24642..25247
                     /locus_tag="VYQ24_000201"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1967391.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEAIVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25234..26433
                     /locus_tag="VYQ24_000202"
     CDS             25234..26433
                     /locus_tag="VYQ24_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1967392.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRIFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26433..26582
                     /gene="rpmG"
                     /locus_tag="VYQ24_000203"
     CDS             26433..26582
                     /gene="rpmG"
                     /locus_tag="VYQ24_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1967393.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26622..26855
                     /gene="secG"
                     /locus_tag="VYQ24_000204"
     CDS             26622..26855
                     /gene="secG"
                     /locus_tag="VYQ24_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1967394.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26957..29311
                     /gene="rnr"
                     /locus_tag="VYQ24_000205"
     CDS             26957..29311
                     /gene="rnr"
                     /locus_tag="VYQ24_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1967395.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29274..29741
                     /gene="smpB"
                     /locus_tag="VYQ24_000206"
     CDS             29274..29741
                     /gene="smpB"
                     /locus_tag="VYQ24_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1967396.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29757..30617
                     /gene="tehB"
                     /locus_tag="VYQ24_000207"
     CDS             29757..30617
                     /gene="tehB"
                     /locus_tag="VYQ24_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1967397.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30690..31643
                     /gene="coiA"
                     /locus_tag="VYQ24_000208"
     CDS             30690..31643
                     /gene="coiA"
                     /locus_tag="VYQ24_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1967398.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKGKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31662..33464
                     /gene="pepF"
                     /locus_tag="VYQ24_000209"
     CDS             31662..33464
                     /gene="pepF"
                     /locus_tag="VYQ24_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1967399.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33466..34179
                     /locus_tag="VYQ24_000210"
     CDS             33466..34179
                     /locus_tag="VYQ24_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1967400.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34246..35187
                     /gene="prsA"
                     /locus_tag="VYQ24_000211"
     CDS             34246..35187
                     /gene="prsA"
                     /locus_tag="VYQ24_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1967401.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT        10623 a         6586 c         7965 g        10152 t
ORIGIN      
        1 gcatttcttt ttaccagaat aattcgctaa ttcttttttt agggcgattg atttttactt
       61 ccgtcacatc aatcattatc gtgtcctcag aactgagagg agttcttgaa atcgtaacac
      121 cactttgaac aagagttact tcaacccatt ggctccgacg gattaagttg ctttcgtgaa
      181 taccaaaatc agccgcaatt tgctcataag ttcgatattc tcgcacatat tgaagagtgg
      241 ccataagaag gtcttctagg cttaatttag gttttcgtcc accttttgcg tgtttaagtt
      301 gataagctgt ttttaataca gctaatatct cttcaaaagt cgtgcgctga acaccaacaa
      361 gacgcttaaa tcgtgcatca gttagttgtt tacttgcttc atcattcata gaactactat
      421 accatatttt gtttcgcagg aagtctattg gaaagtaaga aatattgaag ctgaggcgat
      481 tagaagaaat tgtgagcgtg gtgctatttt ttcaggtaaa ataaaacatc acgaagattc
      541 tcagtttaaa ggagaccact atgttgaatg ttatgctgtt ttagataata cggttatagc
      601 aagagataga ataacagtcc ctatcgatcc gttatgtgga aaagatttta tagagtagca
      661 tataattgat tcttaactgg aatactctct atctctttac atcaagaaaa tgactaaaca
      721 gggaagtttg ccttcttccc tttttttgtt atactagtag aagaaaaaaa tagaaagatt
      781 tgtgggagtg aaaaagtccg ggggactttt tcagcttgag ccaagaaatt cgagagcgag
      841 gaaagtctgg gggacttttt cagcctgagc caagaaactc gaaatctcgt aagtctgatt
      901 ggctttttca atgtgaatct taacttcaca ctcccaaaga ggtattagtg tcgtgtctca
      961 atcttatatc aatgttatcg gtgctggttt ggcaggttct gaagcagctt accaaatcgc
     1021 agagcgtggt attccagtta aactttatga aatgcgtggt gtcaagtcta caccccagca
     1081 taaaacagac aattttgctg agttggtttg ttccaattct ttgcgtgggg atgctttgac
     1141 aaatgcagtt ggtcttctca aggaagaaat gcgtcgcttg ggttctgtta tcttggaatc
     1201 tgctgaggct acacgtgttc ctgccggtgg agcccttgcg gtggatcgtg atggtttctc
     1261 tcaaatggtg accgaaaaag tggtcaacca ccccttgatt gaagtggtgc gtgatgaaat
     1321 tacagaattg ccaacagatg ttattacagt tgtggcgact ggtcccttga ctagcgatgc
     1381 cctggctgaa aagattcatg cccttaataa tggcgatggc ttctatttct acgatgcagc
     1441 agcgcctatt atcgatgtca acactatcga tatgagtaag gtctatctca aatctcgtta
     1501 tgataaggga gaagcagcct atctcaatgc tccaatgacc aagcaagagt ttatggattt
     1561 ccatgaagcc ttggtcaatg cggaagaagc accgctcaat tcttttgaaa aagaaaagta
     1621 ctttgaagga tgtatgccaa tcgaagttat ggccaaacgt ggcattaaaa ctatgcttta
     1681 tggccctatg aagccagtcg gtcttgagta tccggacgac tacacaggac ctcgtgatgg
     1741 agaatttaaa acaccttatg cggttgtgca acttcgtcag gataatgcag ctggtagcct
     1801 atacaatatt gttggtttcc agacccacct caaatgggga gaacaaaagc gtgtcttcca
     1861 aatgattccg ggtcttgaaa atgcggagtt tgtccgttat ggtgtcatgc atcgcaattc
     1921 ttacatggat tcaccaaatc ttcttgagca gacttaccgt tccaagaaac aaccaaatct
     1981 cttctttgct ggtcagatga cgggggtgga aggctatgtt gagtcggcgg cttcgggctt
     2041 agttgcggga attaacgcag ctcgtctctt caaggaagaa agcgaggtta ttttccccga
     2101 gacgacagcg attggaagct tagctcatta cattacccat gccgacagca aacatttcca
     2161 accaatgaat gtcaattttg ggatcatcaa ggagttggaa ggcgagcgta tccgtgataa
     2221 gaaggctcgt tatgaaaaaa ttgcagagcg tgcccttgcc gacttagagg aatttttgac
     2281 tgtctaattt ttttgaaaga attgttcatg atactataaa aatcttagaa attgtgataa
     2341 aataggtagg atgaaagaag gagagtgaaa atggcgaatc ccaagtataa acgtatttta
     2401 atcaagttat caggtgaagc ccttgccggt gaacgtggcg tagggattga tatccaaaca
     2461 gttcaaacaa tcgcaaaaga gattcaagaa gttcatagct taggtatcga aattgccctt
     2521 gttattggtg gaggaaatct ctggcgtgga gaacctgcag cagaagcagg tatggaccgt
     2581 gttcaggcag attacactgg aatgcttggg actgttatga atgctcttgt gatggcagat
     2641 tcattgcaac aagttggggt tgatacgcgt gtacaaacag ctattgctat gcaacaagtg
     2701 gcagagcctt atgtccgtgg acgtgccctt cgtcaccttg aaaaaggccg tatcgttatc
     2761 tttggtgctg gaattggttc accatacttc tcgacagata caacagcggc ccttcgtgca
     2821 gctgaaatcg aagcagatgc catcctcatg gctaaaaatg gcgtcgatgg tgtgtacaat
     2881 gccgatccta agaaggacaa gacagccgtt aagtttgaag aattgaccca ccgtgatgtt
     2941 atcaacaaag gtcttcgtat catggactca acagcctcaa ccctctcaat ggacaacgac
     3001 attgacttgg ttgtcttcaa catgaaccaa tcaggcaaca tcaaacgtgt tgtatttggt
     3061 gaaaatatcg gaacaacagt ttcaaataat atcgaagaaa aggaataaga aagaatatgg
     3121 ctaacgtaat tattgaaaaa gctaaagaga gaatgaccca gtctcaccaa tcacttgctc
     3181 gtgaatttgg tggtatccgt gctggtcgtg ccaatgcaag cttgcttgac cgtgtacatg
     3241 tagaatacta tggagtcgaa actcctctta accaaatcgc ttcaattacg attccagaag
     3301 cgcgtgtttt gttggtaaca ccatttgaca agtcttcatt gaaagacatc gaacgtgcct
     3361 tgaacgcttc tgatcttggt atcacaccgg ctaatgacgg ttctgtgatt cgcttggtta
     3421 tcccagctct tacagaagaa actcgtcgtg accttgctaa agaagtgaag aaggtcggcg
     3481 aaaatgctaa agtggctgtc cgcaatatcc gtcgcgatgc tatggacgaa gctaagaagc
     3541 aagaaaaagc aaaagaaatc actgaagacg aattgaagac tcttgaaaaa gatattcaaa
     3601 aagtaacaga cgatgctgtt aaacacatcg acgacatgac tgctaacaaa gagaaagaac
     3661 ttttggaagt ctaaaaataa acagaaaaac tcagttggca ttgctggctg agttttattc
     3721 gaaagaagga aatatgaata caaatcttgc aagttttatc gttggactga tcatcgatga
     3781 aaatgaccgt ttttactttg tgcaaaagga tggtcaaacc tatgctcttg ctaaggaaga
     3841 gggccaacat acagtagggg atacggtcaa aggttttgca tacacggata tgaagcaaaa
     3901 actccgcctg acaaccttag aagtgactgc cactcaggac caatttggtt ggggacgtgt
     3961 cacagaggtt cgtaaggact tgggtgtctt tgtggataca ggccttcctg acaaggaaat
     4021 cgttgtgtca ctcgatattc tccctgtgct caaggaactc tggcctaaga agggcgacca
     4081 actctacatc cgtcttgaag tggataagaa agaccgtatc tggggcctct tggcttatca
     4141 agaagacttc caacgtctcg ctcgtcctgc ctacaacaac atgcagaacc aaaactggcc
     4201 agccattgtt taccgtctca agctgtcagg aacttttgtt tacctaccag aaaataatat
     4261 gcttggtttt attcatccta gcgagcgtta cgcagagcca cgtttggggc aagtattaga
     4321 tgcgcgcgtt attggtttcc gtgaagtgga ccgcactctg aacctctccc tcaaaccacg
     4381 ctcctttgaa atgttggaaa acgatgctca gatgattttg acttatttgg aaagcaatgg
     4441 cggtttcatg accttaaatg acaagtcatc tccagacgac atcaaggcaa cctttggcat
     4501 ttctaaaggt cagttcaaga aagctttagg tggtcttatg aaggctggta aaatcaagca
     4561 agaccagttt gggacagagt tgatttaggg aggcttatga gaaaatcatt ttacacttgg
     4621 ctcatgaccg agcgcaatcc taaaagtaac agtcccaaag caattttggc agacctcgct
     4681 tttgaagagt cagcctttcc aaaacataca gatgattttg atgaggtcag tcgctttttg
     4741 gaggagcatg ccagtttctc ttttaaccta ggagattttg acagcatttg gcaggaatat
     4801 ctagaacact agcatttatt cattgggttt gggctagtaa tttctccatc cctctgctat
     4861 aataaaaaga aataaaagga ttagagaggt tctttatttg aaggaacatt caatagacat
     4921 tcaactgagt catccagatg acctgtttca tctttttggt tccaatgaac gccatcttcg
     4981 tttgatggaa gaagagcttg atgttgtgat tcatgctcgt acggagattg tccaggtttt
     5041 gggagaagag tctgcctgtg aggaagcccg tcaagttatt caggctttga tggtcttggt
     5101 aaatcgtggg atgaccgttg gtacgccaga tgtagtcact gcgattagca tggtcaaaaa
     5161 tgatgaaatt gacaagtttg tcgcccttta cgaagaagaa atcatcaaag acaatactgg
     5221 gaagcctatc cgagtcaaaa ccttgggtca aaaactttat gtggacagtg tcaaacagca
     5281 tgatgtgacc tttggaatcg gacctgcagg gacagggaag accttccttg cagtgacctt
     5341 ggcagtgact gcccttaaac gtgggcaagt caaacgaatt atcctgacac gcccagcggt
     5401 ggaagcagga gagagtctag gttttcttcc gggtgatctt aaggagaagg tggatcctta
     5461 ccttcgtcct gtttacgatg ccttgtatca aattcttggg aaagaccaaa cgactcgtct
     5521 catggagcgt gaaattatcg aaattgcgcc ccttgcctat atgcgtggcc ggaccttgga
     5581 tgatgccttt gtcattctcg atgaggcgca aaacacgacc atcatgcaga tgaagatgtt
     5641 cttgacgcgt ttaggctttc attctaagat gattgtcaat ggagatatca gtcagattga
     5701 cctgccacgt aatgtcaagt ccggtttgat tgatgctcaa gagaaactca agaacatcca
     5761 tcagattgac tttgttcatt tttcagccaa ggatgtggtt cgccatccag ttgtcgctca
     5821 gattatccga gcctatgaat attctactga agttgcacac gactgatttt gaggaagttc
     5881 gcctgcaaaa gaatatactt ttgagagagg aaaatccagt tgtataggct aaaggtttta
     5941 tccaaaggtt tgagacaacg attaggcacg atggaaagaa cttttatgtg gctgatgacg
     6001 atcagtgcat cttcctgtgt cataatcaca gggcacaaga aagtaggaat ttgaaaagat
     6061 gattgaccaa ctatctaagt attacagttg taggatacta actgaaaagg atattccaag
     6121 tattttatct ttatatgaaa gtaatcctct gtattttcag cattgtccac cagagccaaa
     6181 ttttgcaact gtaaaagagg acatgctttg tctacctgaa ggtaaagcta aggctgataa
     6241 gtttttttgt tggattttgg aatggatctg accttgtggc tgttatggat tttgtctatg
     6301 catatcctga tgaggagact gtttttattg gtttgtttat ggttgatcca gcctatcaga
     6361 ggaaagggat tggtagtcat attgtgacag aagcactagc ttattttgct aagaactttc
     6421 gaaaggcacg tttggcttat gttaagggaa atccgcaatc tcagcatttt tgggaaaagc
     6481 agggctttaa atcaattgga tgcgaggtta agaaagaact ctatacggtt gttatcgttg
     6541 aacagagcct agaagattag aaatggcatc aagtaagaac tatttggaat ttgttttgga
     6601 acaattatca ggattagatg atgtgactta ccgttccatg atgggggagt atattcttta
     6661 cttccgcggc aagattattg gcggcattta tgacgatcgc ttttagttaa acccgtgcaa
     6721 gcagtcttag ataagattga ccaatcttct tttgagtttc catacaaagg tgccaaagaa
     6781 atgatttgag tggaagaact tgataataag atgtttctat aagacctgat tttagctatg
     6841 tataaccaac tgccaacgcc caaacctaaa aagaaaaagc aagggtgaac gaagtaaaaa
     6901 agaagtctgc taaggccctg tctttgcacg ggtaaaattt tatatataaa aagaagctgg
     6961 gactaaagag ctcagcttcc tttggtttat ataattgtca ttacaagacg aagtggttgg
     7021 gcgaaactct gttgacttta ttcaatttag agtttcttat gcacaattga gtctggaacg
     7081 aaagtctcca gttgcaaagt atacagtaca ataaaccaac gatgtaatag ctgatgacac
     7141 aaagcacagt gggtaggact tgcgaagtca cccttttctt ttcaaaattt atactaaatc
     7201 attgatatca gtgtagtcac gattaagtcc ttgagcaact ggtaggctag tcaagtaacc
     7261 ttgataagtg gtcacacctt gacgcaagcc ttcatcttca gagattgctt gtgcgaatcc
     7321 tttgccagcc aaagcttcga tataaggaag agtgacattg gttagggcga tggttgaagt
     7381 gcgggcaacc gcaccaggga tattggcaac ggcatagtgg agaacaccgt gtttttcata
     7441 gacgggttca tcgtgcgttg tcacacggtc agctgtttcg ataaggccac cttggtcaac
     7501 agcaatgtca acgatacaga gcctggacgc atttgtttga ccatctcatc tgtcaccaat
     7561 tccggtgctt ttgcaccagg gatgagaatg gctccaatca ccacatcagc atctctcaca
     7621 cttgcttcaa tgttgaatga attagacata agagtttgaa tttgacttcc aaagacttct
     7681 tctagaactg agagacgctt ggcactaata tctaaaatag tcacttgagc accaagacca
     7741 agggcgatgc gggcagcatg tgtaccgacg acaccatcac cgatgatagt tacttttcct
     7801 tttggaacac ctggtacacc accaagtaga acaccagagc caccagcttg cttagtaagg
     7861 aagtgagctc cgatttaaac agccatacga cctgcaacct cactcatagg aacgaggagc
     7921 ggtagttgtc cttgattgtc acgaacagtt tcataggcaa ttcctgttgt ttttgctgct
     7981 aacatagcat ctgctaattc tggagcagcg gccatgtgca agtaggtgaa gagaagaaga
     8041 tcgtcgcgca agtaaccgta ttcagaactt aaaggttctt ttactttcac aaccaactct
     8101 gctgcccaag cttcaccagc agtagcgaca atctcagctc cttgcttttg atagtcagca
     8161 tcagtaaagc cagaaccgag accagcattt gtttcgataa ggacacgatg accacgacta
     8221 actaagctat gaacacctgc aggtgtgagg gcgacacggt tttcgttatt tttaatttct
     8281 tttgggattc cgattaacat tgagataacc tacctttcaa ttgacggtct tgttttggtt
     8341 gtcacattcc agttcataaa tcaaaaatgt gacggtttca ttgtatatga aaccgcttca
     8401 aaaatcaaga aaaacttgtc atccaaattt tttatgctag actagtgaaa atcaagcttt
     8461 gattggttgg aaaagtatgg aatcaatttt tgtaaaactt gcccagtatc cgtctataga
     8521 aacggagcgt ttattgctta gacctgtaac tttggatgat gcggaagcaa tgtttgacta
     8581 tgcctcggac aagggtaata cacgttacac ttttccaacc aatcaaagct tggaagaaac
     8641 caagaataac attgctcagt tctatttggc taatcccttg ggacgttggg gaatagaact
     8701 aaaaagcaat ggtcagttta ttggaaccat tgacttgcac aagattgatt ctgttcttaa
     8761 gaaggcagct attggctaca ttatcaataa aaagtattgg aatcaaggat taacgacaga
     8821 agccaatcgt gctgtgattg agctagcttt tgagaagata gggatgaata agttgactgc
     8881 ccttcacgat aaggacaatc ctgcgtcagg aaaggtcatg gagaaatcag gcatgcgttt
     8941 ttcccatgca gaaccatatg cttgtatgga ccagcatgaa aaagatcgaa tcgttacaag
     9001 agttcattat gtcttgacca aggaagacta ttttgcaaat aaataagcag ttgaaaagaa
     9061 atttttcgac tgttttttct tcatcttacg aataatctaa gagaggagaa aatatggaag
     9121 caattatcga gaaaatcaaa gagtataaaa tcatcgtcat ctgtactggt ctgggcttgc
     9181 ttgtaggcgg atttttcctg ctaaagccag ctccacaaac acctgtcaaa gaaacgaatt
     9241 tgcaggctga agttgcagct gtttccaagg atttggtatc cgaaaaggaa gtgaagaagg
     9301 aagagaagga agaacccctt gaacaagatc taatcacagt agatgtcaaa ggtgctgtca
     9361 aatcgccagg gatttatgac ttgcctgtag gtagtcgagt caatgatgct gttcagaagg
     9421 ctggtggctt gacagagcaa gcagacagca agtcgctcaa tctagctcag aaagttagtg
     9481 atgaggctct ggtttacgtt cctactaagg gagaagaagc agttagccaa cagactggtt
     9541 tggggacagc ttcttcaata agcaaggaaa agaaggtcaa tctcaacaag gccagtctgg
     9601 aagaactcaa gcaggtcaag ggactgggag gaaaacgagc tcaggacatt atcgaccatc
     9661 gtgaggcaaa tggcaagttc aagtcagtag acgaactcaa gaaggtctct ggcattggtg
     9721 gcaaaacaat agaaaagctt aaagactatg ttacagtgga ttaagaattt ctctattccc
     9781 ctaatttacc tgagttttct attactttgg ctttattacg ctattttctc agcatcttat
     9841 cttgctttgt tgggctttgt ttttctgcta gtctgtctct ttatccaatt tccgtggaaa
     9901 tctgctggta aagttctagt gatttgtgga atctttggat tttggtttgt ttttcaaaat
     9961 tggcaacaga gtcaagcgag tcaaaatctg gcggattctg ttgaaggggt acggattttg
    10021 cctgatacta ttaaggttaa tggtgatagt ctatcctttc gtggcaagtc taacggtcgt
    10081 gctttccaag tctattataa actccagtcc gaggaggaga aagaagcctt tcaagcttta
    10141 accgacctgc atgagatagg actagaaggg aagctttcgg agccagaagg gcagagaaat
    10201 tttggtggct ttaattacca agcctatctg aagactcagg gaatttacca gactctcaat
    10261 atcaaaacaa tccagtcact tcaaaagatt ggcagttggg atataggaga aaacttgtcc
    10321 agtttacgtc gaaaggctgt ggtttggatt aagacgcact ttccagaccc tatgcgcaat
    10381 tacatgacag gactcttgct gggacatctg gacaccgact ttgaggagat gaatgagctt
    10441 tattccagtc taggaattat ccacctcttt gccctatctg gtatgcaggt aggttttttc
    10501 atgaatggat ttaagaaact tctcttgcga ttgggcttga cccaagaaaa gttgaaatgg
    10561 ctgacttatc ccttttccct tatctatgcg ggactaactg gattttcagc atcggttatt
    10621 cgcagtctct tgcaaaagct actggctcaa catggggtta agggcttgga taattttgcc
    10681 ttgacggtgc ttgtcctctt tattgtcatg ccaaactttt tcttgacagc aggaggagtc
    10741 ttgtcctgcg cttatgcttt tatcctgacc atgaccagca aagaagggga ggggctcaag
    10801 gctgttacta gtgaaagtct agtcatctcc ttgggcatat tgcccattct atccttctat
    10861 tttgcggaat ttcaaccttg gtctatcctt ttgacctttg tcttttcctt tctatttgac
    10921 ttgaccttct taccgctctt gtctatttta tttgtccttt cctttctcta tccagtcatt
    10981 cagctgaact ttatttttga atggttggag ggcattattc gcttggtgtc acaggtgaca
    11041 agtagacctc tggtctttgg acaacccaat gcatggtttt taatcctatt gttaatttcc
    11101 ttggctttgg tctatgattt gagaaaaaac attaaaaagc taacggtatt gtgcttattg
    11161 attacagggc tctttctcct gaccaagcat ccactggaaa atgaaatcac catgctggat
    11221 gtggggcaag gagaaagtat tttcctacgg gatgtaactg ggaaaaccat tctcatagat
    11281 gtaggtggta aggcagaatc ttataagaaa atcgaaaaat ggcaagaaaa gatgacgacc
    11341 agcaatgccc agcgaacctt gattccctat ctcaaaagtc gaggagtagc taagattgac
    11401 cagctaattt tgactaacac ggacaaggag aatgttggag atttgtcaga ggtgaccaag
    11461 gctttccatg taggggagat tctagtatca aaagacagtc tgaaacagaa ggaatttgtg
    11521 gcagaactac aggcgactca aacaaaggtg cgtagtatga cagtagggga gaacttgccc
    11581 atttttggaa gtcagttaga agttctatct ccaaggaaaa tgggagatgg aggacacgat
    11641 gataccctag ttctgtatgg gaaattcttg gataagcaat ttctcttcac gggaaatttg
    11701 gaggagaaag gagagaagga cttgctgaag cactatccag acttgaaagt aaatgttttg
    11761 aaagctagcc aacatggcaa taaaaaatca tcaagtccag cctttctaga aaaactcaaa
    11821 ccagagctta ctcttatctc agttggaaag agcaatcgaa tgaaactccc ccatcaggaa
    11881 acattgacac gactggaagg tatcaatagc aaagtttatc gaactgacca gcaaggagct
    11941 atacgtttta aggggttgga tagttggaaa atcgaaagtg ttcgatagga aggataaatg
    12001 ttgtagatta gtgaaataaa ctaaaaattt gttgcataat aatgataaaa atggtataat
    12061 gaaaacgcat tcaatattga ggatataaaa tcattaaaaa tcagcaaaag ttgttttatt
    12121 agttagttta taatctattg gtcttcttca gtccagtgta tctgctgtga cagtcactaa
    12181 aagttacaag tatgattgga atacggtttg ggaatatagt accaactatc acgaccatca
    12241 gtatgcttgg attccgtcat ggtctcgtta tgacagctat tctgagtata aagttggcgg
    12301 aggctggaac tacgctcgtt atgaggtcat aaactattac agcggaggct attaattctt
    12361 aaagagtgag aaaaaggagg gctagatatg ttgcagctta ctcatgtgac cttaaaaacg
    12421 cgacaagtca tcttgcaaga tgtggatttc acctttaaaa agggtagggt ttatggtctt
    12481 cttgctatca atggctctgg aaagacgacc ctgttccgtg ccattagcaa tttaattccc
    12541 ataagtagtg gaaatatcgc agcccctcct tctttatttt attatgagag tattgaatgg
    12601 ctggatggaa acttaagtgg gatggactac cttcgtctta tcaaaaacat ctggaagtca
    12661 ggtctgaact tgagggatga aatcgcctat tgggaaatgt ctgactatat cagtcttccc
    12721 attcgcaagt attccttagg catgaagcaa cgcttggtga ttgccatgta tttcctcagt
    12781 caggccaaat gctggctcat ggatgagatt acaaatggct tagatgagta ttatcgacag
    12841 aagttttttg ataggctagc acaaatcgat agacaagaac agctggttct tttaagttcc
    12901 cactataagg aagagttggt tgatgtctgc gatagagtag taaccattca tcaggggcag
    12961 atagaagagg tttagtttat gaaagatgtt agtctatttt tattgaaaaa agttttcaaa
    13021 agccgcttaa actggattgt cttagcttta tttgtatctg tactcggtgt taccttttat
    13081 ttaaatagtc agactgcaaa ctcacacagc ttggagagca ggttggaaag tcgcattgca
    13141 gccaacgaga gggctatcaa tgaaaatgaa gagaaactct cccaaatgtc tgataccagc
    13201 tcggaggaat accagtttgc taaaaataat ttagacgtgc aaaaaaatct tttgacgcga
    13261 aagacagaaa ttctaacttt actaaaagaa gggcgctgga aagaagccta ctatttgcag
    13321 tggcaagatg aagagaagaa ttatgaattt gtatcaaatg acccgactgc tagccctggc
    13381 ttaaaaatgg gggttgaccg cgaacggaag atttaccaag ccctgtatcc cttgaacata
    13441 aaagcacata ctttggagtt tccgacccac gggattgatc agattgtctg gattttagag
    13501 gttatcatcc caagtttgtt tgtggttgct attattttta tgctaacaca actatttgca
    13561 gaaagatatc aaaatcatct ggacacagct cacttatatc ctgtttcaaa agtgacattt
    13621 gcaatatcct ctcttggagt tggagtggga tatgtaactg tgctgtttat cggaatctgt
    13681 ggcttttctt ttctagtggg aagtctgata agtggttttg gacagttaga ttatccctac
    13741 ccaatttata gcttagtgaa tcaagaagta actattggga aaatacaaga tgtattattt
    13801 cctggcttgc tcttagcttt cttagccttt atcgtcattg tggaagttgt gtacttgatt
    13861 gcttactttt tcaagcaaaa aatgcctgtc ctctttcttt cactcattgg gattgttggc
    13921 ttattgtttg gtatccaaac cattcagcct cttcaaagga ttgcacatct gattcccttt
    13981 acttacttgc gttcagtgga gattttatct ggaagattac ctaagcagat tgataatgtc
    14041 gatctaaatt ggagcatggg aatggtctta cttccttgcc tgattatctt tttgctattg
    14101 ggaattctat ttattgaaag atggggaagt tcacagaaaa aagaattttt taatagattc
    14161 tagctttcct ataggtaggg aaaataagta aaaactaaca tagagaggga atcaacttga
    14221 ttctctcttt ttgattcgaa aaccaaacca aaatacaaac acaaactttt caaaaaataa
    14281 ctttttatct tgacaagagc tagaaaactt ggtatcatat aaaagttgag aaaagcagaa
    14341 gtgagagctt ctcgccttgt gacattaagt tgcctggccc tacggatgaa aagtttcgaa
    14401 gaaacgctat cataacgtgc gggcttgtat atttacaagt ccgctattgt ttttctctaa
    14461 taaaacaaaa gaggtgaaaa ccatagcaaa gcaagactta ttcatcaatg atgagattcg
    14521 tgtacgtgaa gttcgcttga ttggtcttga aggagaacag ctaggtatca agccactcag
    14581 tgaagcgcaa gctttggctg ataacgctaa tgttgaccta gtattgattc aaccccaagc
    14641 caaaccgcct gttgcaaaaa ttatggacta cggtaagttc aaatttgagt accagaagaa
    14701 gcaaaaagaa caacgtaaaa aacaaagcgt tgttactgtg aaagaagttc gtctaagtcc
    14761 aactattgac aagggtgact ttgatacaaa acttcgtaat gcacgtaagt tccttgaaaa
    14821 aggaaataaa gttaaggtat ctattcgctt taagggtcgt atgattaccc ataaagagat
    14881 tggtgcaaaa gttttagccg agtttgctga agcaactcaa gatattgcca tcatcgaaca
    14941 acgtgctaaa atggatggac gtcaaatgtt catgcaattg gcgccagcga ctgacaaaaa
    15001 ataattgtca gaaagttaaa taaaggagaa aaaatcatgc caaaacaaaa aacacaccgc
    15061 gcatcagcta aacgtttcaa acgtacaggt tctggtggac ttaaacgttt ccgtgcttac
    15121 acttctcacc gtttccacgg aaaaactaag aaacaacgtc gtcatcttcg taaagcatct
    15181 atggtgcatt caggagatta caaacgtatc aaagcaatgc ttactcgctt gaaataatag
    15241 cttgactgta agtaaacaat tctaggaaat atttggagga aataaaacat ggcacgtgtt
    15301 aaaggtggcg ttgtatcacg caaacgtcgt aaacgtattc ttaaattagc aaaaggttac
    15361 tatggagcta aacacatctt gttccgtact gcaaaagaac aagtaatgaa ctcttactac
    15421 tatgcatacc gtgaccgtcg tcagaaaaaa cgtgacttcc gcaaattgtg gattactcgt
    15481 atcaatgcgg cagctcgtat gaacggactt tcatactcac aattgatgca tggtttgaaa
    15541 ttggctgaga tcgaagttaa ccgtaaaatg cttgctgact tggctgttaa cgatgcagta
    15601 gctttcacag ctcttgcaga tgcagctaaa gcaaaacttg gtaaataaca actgatgaga
    15661 ctggaacagg attgtccagt cttttttaaa ataaaggaga aagatatggc ttcaaaaatg
    15721 ctacacactt gcttacgagt agaaaatctt gaaaaatcaa tcgcatttta tcaagatgct
    15781 tttggtttta aagaattgcg tcgcagagat tttccagacc atgccttcac gattgtctat
    15841 ctaggtcttg agggtgatga ttatgagttg gagttgactt ataactacga tcacggtccg
    15901 tatgtggttg gtgatgggtt tgcccatatc gccctcagta cacctgatct tgaggcactt
    15961 catcaagaac acagtgcaaa aggctatgaa gtgactgagc caaatggtct accaggaact
    16021 acaccaaact attactttgt taaagaccct gatggatata aggtcgaagt cattcgtgaa
    16081 aaataatttc gtgcaagtta agtagaatat tcgttttcta cttgactttt tttagctgaa
    16141 agagtatact aataaaaatt caacctttaa ggggagtcca gagagactca caaggtgtca
    16201 gataaaagaa tagtgcaatt ttctagagga gaccttttga gtgtgctctc ttgtgttgta
    16261 cgattttaac tgaggccttg cactagcaag gtcttttctt tatctggtcc ccttaaaatt
    16321 taaggaggaa aagttatgaa tctcacatgt aagaagcgtt tgggtgtcat tcggttggaa
    16381 accatgaagg tggttgcaca agaggaaatc gcgccagcaa tctttgaatt agtcctagaa
    16441 ggagaaatgg ttgaagccat gcgagcaggc caatttcttc atctgcgtgt accagacgat
    16501 gcccatctct tacgtcgtcc tatttcaatt tcgtctattg acaaggcaaa caagcagtgt
    16561 cacctcattt atcggattga gggagctggg actgcaattt tttcaacctt aagtcaggga
    16621 gacactcttg atgtgatggg tcctcaggga aatggttttg acttgtctga ccttgatgag
    16681 cagaatcagg ttctccttgt tggtggtggg attggtgttc cacccttgct tgaggtggcc
    16741 aaggaattgc atgaacgtgg agtgaaagta gtgacagtcc tcggttttgc taataaggat
    16801 gctgttattt tgaaaacgga attggctcag tatggtcagg tctttgtaac gacagatgat
    16861 ggttcttatg gcatcaaggg aaatgtttcc gttgttatca atgatttaga tagtcagttt
    16921 gacgctgttt actcgtgtgg ggctccagga atgatgaagt atatcaatca aacctttgat
    16981 gatcacccaa gagcctattt atctctggaa tctcgtatgg cttgtgggat gggagcttgc
    17041 tatgcctgtg ttctaaaagt accagaaagc gagacggtca gccaacgcgt ctgtgaagat
    17101 ggtcctgttt tccgcacagg aacagttgta ttataaggag aaaattatga ctacaaatcg
    17161 attacaagtg tctctacctg gtttggattt gaaaaatccg attattccag catcaggctg
    17221 ttttggcttt ggacaagagt atgccaagta ctatgattta aaccttttag gttctattat
    17281 gatcaaggcg acaacccttg aaccacgttt tgggaaccca actccaagag tagcagagac
    17341 gcctgctggt atgctcaatg caattggctt gcaaaatcct ggtttagagg ttgttttggc
    17401 tgaaaagtta ccttggctgg aaagagaata tccaaatctt cctattattg ccaatgtagc
    17461 tggtttttca aaacaagagt atgcagctgt ttctcatggg atttccaagg caactaatgt
    17521 aaaagctatc gagctcaata tttcttgtcc caatgttgac cactgtaatc atggactttt
    17581 gattggtcaa gatccagatt tggcttatga tgtggtgaaa gcagctgtgg aagcctcaga
    17641 agtgccagtt tatgtcaaat taaccccgag tgtgaccgat atcgttactg tcgcaaaagc
    17701 tgcagaagat gcgggagcaa gtggcttgac catgatcaat actctggttg gaatgcgctt
    17761 tgacctcaaa accagaaaac caatcttggc caatggaaca ggtggaatgt caggtccagc
    17821 agtctttcca gtagccctca aactcatccg ccaagtagcc caaacaacag acctgcctat
    17881 cattggaatg gggggagtgg attcggctga agctgcccta gaaatgtatc tggctggggc
    17941 atctgctatc ggagttggaa cagctaactt taccaatcct tatgcctgcc ctgacatcat
    18001 cgaaaattta ccaaaagtca tggataaata cggtattagc agtctggaag aactccgtca
    18061 ggaagtaaaa gagtctctga ggtaaactgc aatcaatctg ttcttgattt tttattagtt
    18121 tgtaatatga atttaggaga attttggtac aataaaataa ataagaacag aggaagaagg
    18181 ttaatgaaga aagtaagatt tattttttta gctctgctat ttttcttagc tagtccagag
    18241 ggtgcaatga ctagtgatgg tacttggcaa ggaaaacagt atctgaaaga agatggcagt
    18301 caagcagcga atgagtgggt ttttgatact cattatcaat cttggttcta tataaaagca
    18361 gatgctaact atgctgaaaa tgaatggcta aagcaaggtg acgactattt ttacctcaaa
    18421 tctggtggct atatggccaa atcagaatgg gtagaagaca agggagcctt ttattatctt
    18481 gaccaagatg gaaagatgaa acgaaatgct tgggtaggaa cttcctatgt tggtgcaaca
    18541 ggtgccaaag taatagaaga ctgggtctat gattctcaat acgatgcttg gttttatatc
    18601 aaagcagatg gacagcacgc agagaaagaa tggctccaaa ttaaagggaa ggactattat
    18661 ttcaaatccg gtggttatct actgacaagt cagtggatta atcaagctta tgtgaatgct
    18721 agtggtgcca aagtacagca aggttggttt tttgacaaac aataccaatc ttggttttac
    18781 atcaaagaaa atggaaacta tgctgataaa gaatggattt tcgagaatgg tcactattat
    18841 tatctaaaat ccggtggcta catggcagcc aatgaatgga tttgggataa ggaatcttgg
    18901 ttttacctca agtttgatgg caaaatggct gaaaaagaat gggtctacga ttctcatagt
    18961 caagcctggt actacttcaa atccggtggt tacatgacag ccaatgaatg gatttgggat
    19021 aaggaatctt ggttttatct caaatctgat gggaaaatag ctgaaaaaga atgggtctac
    19081 gattctcata gtcaagcttg gtactacttc aaatccggtg gttacatgac agccaatgaa
    19141 tggatttggg ataaggaatc ttggttttac ctcaagtctg atggcaaaat ggctgaaaaa
    19201 gaatgggtct acgattctca tagtcaagcc tggtactact tcaaatctgg tggctacatg
    19261 gcgaaaaatg agacagtaga tggttatcag cttggaagcg atggtaaatg gcttggagga
    19321 aaagctacaa ataaaaatgc tgcttactat caagtagtgc ctgtcacagc caatgtttat
    19381 gattcagatg gtgaaaagct ttcctatata tcgcaaggta gtgtcgtatg gctagataag
    19441 gatagaaaaa gtgatgacaa gcgcttggct attactattt ctggtttgtc aggctatatg
    19501 aaaacagaag atttacaagc gctagatgct agtaaggact ttatccctta ttatgagagt
    19561 gatggccacc gtttttatca ctatgtggct cagaatgcta gtatcccagt agcttctcat
    19621 ctttctgata tggaagtagg caagaaatat tattcggcag atggcctgca ttttgatggt
    19681 tttaagcttg agaatccctt ccttttcaaa gatttaacag aggctacaaa ctacagtgct
    19741 gaagaattgg ataaggtatt tagtttgcta aacattaata atagcctttt ggagaacaag
    19801 ggcgctactt ttaaggaagc cgaagaacat taccatatca atgcccttta tctccttgcc
    19861 catagtgccc tagaaagtaa ctggggaaga agtaaaattg ccaaagataa gaataatttc
    19921 tttggcatta cagcctatga tacgacccct tacctttctg ccaagacatt tgatgatgtg
    19981 gataagggaa ttttaggtgc aaccaagtgg attaaggaaa attatatcga taggggaaga
    20041 actttccttg gaaacaaggc ttctggtatg aatgtggaat atgcttcaga cccttattgg
    20101 ggcgaaaaaa ttgctagtgt gatgatgaaa atcaatgaga agctaggtgg caaagattag
    20161 tactataagt gaatatgatt tgagtgaata gtaagttaaa aatcctgatt tcaagtaaaa
    20221 tcaggatttt ttcatggatg caattttttt ggagtctggt gtgacgcgga gggtcttttg
    20281 tcctgtgtaa gtgacaaagc cgggttttcc accagttggt ttattgagtt ttttgacttc
    20341 aatcatatct acctgcacca gattcgacag gcgcccttga gagaagtagg cagctaactc
    20401 tgctgcgtct gtcttgactg catcagatgg gtcaagattt cctgagatga caacatggct
    20461 tccaggaatg tccttagcat ggaaccaaag ttcctccttg cgggccattt taaaggtcaa
    20521 ttcctcattt tgaagattgt ttcgtccgac atagatgatg gttttgccat cgcttgctag
    20581 atattgttct agttttttgc gtttctggat tttctcccgt tgtcttctgc ggataaaacc
    20641 tgtttgaatc aattcttcac ggatttcagc gatttcttcc agtccagctt ggttgaggac
    20701 ggtttctaca ctttccagat agagaatagt ggctttggtt tcttcaatca aatcagtcaa
    20761 gtatttgaca gcttctttga gtttctgata ccgtttaaaa tagcgttggg cattctggtt
    20821 gggagtcaga gccttatcaa gcgcaatcat gataggttgg ttggtatagt agttgtctag
    20881 gataacctgg tcttggtcgt taggcacttg gtggaggaag gttgtcagca attctccttt
    20941 ttgacgaaat tcttcagcgt tgtctgtcgc cagtaactct ttttcctgtt ttttgagttt
    21001 gtgtcggttt ttctgaagtt cattttcaac acgacgaatc agttcactgg cctgctgttt
    21061 gacgcggtcg cgctcagcct tatccttata gtaggtgtcc aacaaatcag aaagatttgc
    21121 aaaaggctct cccacctgat ttgcaaaagg aactggactg aaggaagtct cagtcaagca
    21181 tggcttggtt tcctgattga aaaaatttcg gaaagcggaa agtttttcac taaccagtat
    21241 cctttccaat tcatttgccg tatcgcgtcc cagaccttga aagaggcttt gaagattttt
    21301 tgctgttagt tcttgggttt gcaggatttc aaagagcttt tcatccttga tagtaaaagg
    21361 attgagagat tctgtacttg gcggagcgat ataggtcgat cctggaagta aggtgcggta
    21421 gctattttgt gaaaaaccga cgtgtttgat aacttcgagg attttatgac tgcttttatc
    21481 gaccagtaga atattactgt gtttccccat aatttcgata atcaaggtag cctggatatg
    21541 gtctccaatc tcgtttttat tggaaactgt catttccaca atacggtcat tttccacttg
    21601 ctcaatcgac tcaatcaggg ccccctgcaa atactttctc aaaaccatga taaaggtaga
    21661 aggttgggct ggattttcaa aagtcgtttg ggtcagctga atgcgtccaa aaactggatg
    21721 ggcagaaagg agcaggcgat ggctttggcg attgctgcgg atttgcaaga ccaactcttg
    21781 ttcaaaaggc tgattgattt tctggatgcg accattcact aattcgcttc gcaattcctc
    21841 aactatgtgg tgtaaaaaaa atccgtcaaa tgacatcgtt ctctccttgt gattgtattc
    21901 catagtatta tatcaaaaag gtagaataaa atcatggaaa tgtggtataa taaagccaag
    21961 taaagagaaa cgagaagcac atgtatattg aaatggtaga tgaaactggt caagtttcaa
    22021 aagaaatgtt gcaacaaacc caagaaattt tggaatttgc agcccaaaaa ttaggaaaag
    22081 aagacaagga gatggcagtc acttttgtga ccaatgagcg tagtcatgaa cttaatctgg
    22141 agtaccgtga caccgaccgt ccgacagatg tcatcagcct tgagtataaa ccagaattgg
    22201 aaattgcctt tgacgaagag gatttgcttg aaaatccaga attggcagag atgatgtctg
    22261 agtttgatgc ctatattggg gaattgttca tctctatcga taaggctcat gagcaggccg
    22321 aagaatatgg tcacagcttt gagcgtgaga tgggcttctt ggcagtacac ggctttttac
    22381 atattaacgg ctatgatcac tacactccgg aagaagaagc ggagatgttc ggtttacaag
    22441 aagaaatttt gacagcctat ggactcacaa gacaataaac gaaaatggaa aaatcgtgac
    22501 ctgatatcca gtttagaatt tgccatcaca gggattttta ctgctatcaa ggaagaacga
    22561 aatatgcgaa aacatgcagt gacggctcta gtggttatcc ttgcaggttt tgtttttcag
    22621 gtgtcacgaa tcgaatggct ctttctccta ttgagcattt tcttggtagt agcctttgag
    22681 attatcaact ctgctattga aaatatggtg gatttggcca gtcactatca cttttccatg
    22741 ctggctaaaa atgccaagga tatggcggct ggcgcggtat tagtggtttc tcttttcgca
    22801 gccttaacag gtgcattgat ttttctccca cgaatctggg atttattatt ttaaacagta
    22861 agaggaaatt atgactttta aatcaggctt tgtagccatt ttaggacgtc ccaatgttgg
    22921 gaagtcaacc tttttaaatc acgttatggg gcaaaagatt gccatcatga gtgacaaggc
    22981 gcagacaacg cgcaataaaa tcatgggaat ttacacgact gataaggagc aaattgtctt
    23041 tatcgacaca ccagggattc acaaacctaa aacagctctc ggagatttca tggttgagtc
    23101 tgcctacagt acccttcgcg aagtggacac cgttcttttc atggtgcctg ctgatgaagc
    23161 gcgtggtaag ggggacgata tgattatcga gcgtctcaag gctgccaagg ttcctgtgat
    23221 tttggtggtg aataaaatcg ataaggtcca tccagaccag ctcttgtctc agattgatga
    23281 cttccgtaat caaatggact ttaaggaaat tgttccaatc tcagcccttc agggaaataa
    23341 cgtgtctcgt ctagtggata ttttgagtga aaatctggat gaaggtttcc aatatttccc
    23401 gtctgatcaa atcacagacc atccagaacg tttcttggtt tcagaaatgg ttcgcgagaa
    23461 agtcttgcat ctaactcgtg aagagattcc gcattctgta gcagtagttg ttgactctat
    23521 gaaacgagac gaagagacag acaaggttca catccgtgca accatcatgg ttgagcgcga
    23581 tagccaaaaa gggattatca tcggtaaagg tggcgctatg cttaagaaaa tcggtagcat
    23641 ggcccgtcgt gatatcgaac tcatgctagg agacaaggtc ttcctagaaa cctgggtcaa
    23701 ggtcaagaaa aactggcgcg ataaaaagct agatttggct gactttggct ataatgaaag
    23761 agaatactaa gtagaggtag gctcatgcct gcttcttgtt tttacagaag gagacttatg
    23821 cctgaattac ctgaggttga aaccgtttgt cgtggcttag aaaaattgat tataggaaag
    23881 aagatttcga gtatagaaat tcgctacccc aagatgatta agacggattt ggaagagttt
    23941 caaagggaat tgcctagtca gattatcgag tcaatgggac gtcgtggaaa atatttgctt
    24001 ttttatctga cagacaaggt cttgatttcc catttgcgga tggagggcaa gtatttttac
    24061 tatccagacc aaggacctga acgcaagcat gcccatgttt tctttcattt tgaagatggt
    24121 ggcacgcttg tttatgagga tgttcgcaag tttggaacca tggaactctt ggtgcctgac
    24181 cttttagacg cctactttat ttctaaaaaa ttaggtcctg aaccaagcga acaagatttt
    24241 gatttacagg tctttcaatc tgcccttgcc aagtccaaaa agcctatcaa atcccatctc
    24301 ctagaccaga ccttggtagc tggacttggc aatatctatg tggatgaggt tctctggcga
    24361 gctcaggttc atccagctag accttcccag actttgacag cagaagaagc gactgccatt
    24421 catgaccaga ccattgctgt tttgggccag gctgttgaaa aaggtggctc caccattcgg
    24481 acttatacca atgcctttgg ggaagatgga agcatgcagg actttcatca ggtctatgat
    24541 aagactggtc aagaatgtgt acgctgtggt accatcattg agaaaattca actaggcgga
    24601 cgtggaaccc acttttgtcc aaactgtcaa aggagggact gatgggaaaa atcatcggaa
    24661 tcactggggg aattgcctca ggtaagtcaa ctgtgacaaa ttttctaaga cagcaaggct
    24721 ttcaagcagt ggatgccgac gcagtcgttc accaactaca gaaacctggt ggtcgtctgt
    24781 ttgaggctat agtacagcac tttgggcaag aaatcattct tgaaaacgga gaactcaatc
    24841 gccctctcct agctagtctc atcttttcaa atcctgaaga gcaaaaatgg tctaatcaaa
    24901 ttcaagggga gattatccgt gaggaactgg ctactttgag agaacaattg gctcagacag
    24961 aagagatttt cttcatggat attcccctac tttttgagca ggactacagc gattggtttg
    25021 ctgagacttg gttggtctat gtggaccgag atgcccaagt agaacgctta atgaaaaggg
    25081 accagttgtc caaagatgaa gctgagtctc gtctggcagc ccagtggcct ttagaaaaaa
    25141 agaaagattt ggccagccag gttcttgata ataatggcaa tcagaaccag cttcttaatc
    25201 aagtgcatat ccttcttgag ggaggtaggc aagatgacag agattaactg gaaggataat
    25261 ctgcgtattg cctggtttgg taattttctg acaggagcca gtatttcttt ggttgtacct
    25321 tttatgccca tcttcgtgga aaatctaggt gtagggagtc agcaagtcgc tttttatgca
    25381 ggcttagcaa tttctgtctc tgctatttcc gcggcgctct tttctcctat ttggggtatt
    25441 cttgctgaca aatacggccg aaaacccatg atgattcggg caggtcttgc tatgactatc
    25501 actatgggag gcttggcctt tgtcccaaat atctattggt taatctttct tcgtttacta
    25561 aacggtgtat ttgcaggttt tgttcctaat gcaacggcac tgatagccag tcaggttcca
    25621 aaggagaaat caggctctgc cttaggtact ttgtctacag gcgtagttgc aggtactcta
    25681 actggtccct ttattggtgg ctttatcgca gaattatttg gcattcgtac agttttctta
    25741 ctggttggta gttttctatt tttagctgct attttgacta tttgctttat caaggaagat
    25801 tttcaaccag tagccaagga aaaggctatt ccaacaaagg aattgtttac ttcagttaaa
    25861 tatccctatc ttttgctcaa tctcttttta accagttttg tcatccaatt ttcagctcaa
    25921 tcgatcggcc ctattttggc tctttatgta cgcgacttag ggcagacaga gaatcttctt
    25981 tttgtctctg gtttgattgt gtccagtatg ggcttttcca gcatgatgag tgcaggagtc
    26041 atgggcaagc taggtgacaa ggtgggcaat catcgtctct tggttgtcgc ccagttttat
    26101 tcagtcatca tctatctcct ctgtgccaat gcctctagcc cccttcaact aggactctat
    26161 cgtttcctct ttggattggg aaccggtgcc ttgattcccg gggttaatgc cctactcagc
    26221 aaaatgactc ccaaagccgg catttcgagg atctttgcct tcaatcaggt attcttttat
    26281 ctgggaggtg ttgttggtcc catggcaggt tctgcagtag caggtcaatt tggctaccat
    26341 gctgtctttt atgcgacaag cctttgtgtt gcctttagtt gtctctttaa cctgattcaa
    26401 tttcgaacat tattaaaagt aaaggaaatc tagtgcgagt aaaaattaat ctcaaatgct
    26461 cctcttgtgg cagtatcaat tacctaacca gtaaaaattc aaaaacccat ccagacaaga
    26521 ttgaggtttt aaagtattgt cccaaggaaa gaaaagtaac cctacatctt gaatctaagt
    26581 agattttatg gtaaaataat agggatttta aggagtttga tatgtataac ctattattaa
    26641 ccattttatt agtattatct gttgtgattg tgattgcaat tttcatgcaa ccaaccaaaa
    26701 accaatccag caatgtattt gatgccagtt caggtgattt gtttgaacgc agtaaagctc
    26761 gcggttttga agctgtaatg cagcgtttga cagggatttt agtctttttc tggctagcca
    26821 ttgccttagc attgacggta ttatcaagta gataagaaaa taatgggcag gactaggtct
    26881 ttgcctcttt ttatttttaa aggatgtttg agaaggtttt acagtaaaag aaaattaaaa
    26941 aatctagaaa gaaaatatga aagatagaat aaaagaatat ttacaagaca agggaaaggt
    27001 gactgttaat gatttggctc aggctttggg aaaagacagt tccaaggatt ttcgtgagtt
    27061 gattaaaacc ttgtccttaa tggaaagaaa gcaccagatt cgctttgaag aagatggtag
    27121 tctgacatta gaaattaaga aaaaacatga gattaccctc aaggggattt ttcatgccca
    27181 taaaaatggc tttggctttg ttagtctgga aggcgaggag gacgaccttt ttgtagggaa
    27241 aaatgatgtc aactatgcta ttgatggtga taccgtcgag gtagtgatta agaaagtcgc
    27301 tgaccgcaat aagggaacag cagcagaagc caaaattatt gatatcctag aacacagttt
    27361 gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaag cctaagtatg ctggctatat
    27421 tcgttcaaaa aatcagaaaa tcagtcaacc gatttatgtt aagaaaccag ccctaaagtt
    27481 agaaggaaca gaagttctca aagtctttat cgataaatac ccaagcaaga aacatgattt
    27541 ctttgtcgcg agtgttctcg atgtagtggg gcactcaacg gatgtcggaa ttgatgttct
    27601 tgaggtcttg gaatcaatgg acattgtatc tgagtttcca gaagctgttg tcaaggaagc
    27661 agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg atctaagaga
    27721 tgaaattacc tttaccattg acggtgcgga tgccaaggac ttggacgatg cagtgcatat
    27781 caaggctctg aaaaatggca atctggagct tggggttcac atcgcagatg tttcctatta
    27841 tgtgaccgag ggttctgccc ttgacaagga agcccttaac cgtgcgactt ctgtttacgt
    27901 gacagaccga gtggtgccaa tgcttccaga acgactatca aatggcatct gctctctcaa
    27961 tccccaagtt gaccgcctga cccagtctgc tattatggag attgataaac atggtcgtgt
    28021 ggtcaactat accattacac aaacagttat caagaccagt tttcgtatga cctatagcga
    28081 tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa gaataccata aaattgtacc
    28141 aagtatcgaa ctcatggcca agcttcatga aactttagaa aacatgcgtg tgaaacgtgg
    28201 agctctcaat tttgatacca atgaagcgaa gattttagtg gataaacaag gtaagcctgt
    28261 tgatatcgtt cttcggcagc gtggcattgc cgagcggatg attgagtctt ttatgttgat
    28321 ggctaatgaa acagttgccg aacatttcag caagttggat ttgcctttta tctatcgaat
    28381 tcacgaggag cctaaggctg aaaaggttca gaagtttatt gattatgctt cgagttttgg
    28441 cttgcgcatt tatgggactg ccagtgagat tagccaggag gcgcttcaag acatcatgcg
    28501 tgctgttgag ggagaacctt atgcagatgt attgtccatg atgcttcttc gctctatgca
    28561 gcaggctcgt tattcggagc acaatcacgg ccactatgga ctagctgctg actattatac
    28621 tcactttacc agtccaattc gtcgttatcc agaccttctt gttcaccgta tgattcggga
    28681 ttacggctgt tctaaggaaa tagcagagca ttttgaacaa gtgattccag agattgcgac
    28741 ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag cgtgaagttg aagccatgaa
    28801 aaaggctgag tatatggaag aatacgtggg tgaagagtat gatgcagttg tatcaagtat
    28861 tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt gaaggcttga ttcacatcac
    28921 taatctacct gaattttatc atttcaatga gcgtgatttg actcttcgtg gagaaaaatc
    28981 aggtatcact ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag cggataaaat
    29041 gactggagag attgattttt cattcgtacc tagtgagttt gatgtgattg aaaaaggctt
    29101 gaaacagtct agtcgtagtg gcagagggcg tggttcaaat cgtcgttcgg ataagaagga
    29161 agacaagaga aaatcaggac gctcaaatga taagcgtaag cattcacaaa aagacaagaa
    29221 gaaaaaagga aagaaacctt tttacaagga agtagctaag aaaggagcca agcatggcaa
    29281 agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat acaatcgtag
    29341 atacgctaga ggcagggatg gtcctgactg gaactgaaat caagagtgta cgagctgctc
    29401 gaattaatct caaggatggc tttgctcaag tgaaaaatgg agaagtttgg ctgagtaatg
    29461 ttcatatcgc gccttacgaa gagggcaata tctggaacca ggaaccagaa cgtcgtcgta
    29521 aactcctgct ccataaaaag caaattcaaa aattggaaca agagaccaaa gggacaggaa
    29581 tgaccttagt tccccttaag gtctatataa aagatggcta cgctaagctt cttttaggac
    29641 ttgccaaagg gaagcatgac tatgacaaac gggagtctat caaacgtcgt gagcaaaatc
    29701 gagatatcgc gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa ttgaaaatgg
    29761 aaaaattagt tgcctataaa cgcatgcctt tgtggaataa acaaacaatg cctgaagctg
    29821 ttcagcaaaa gcacaataca aaagttggga cttgggggaa aattactgtc ttgaagggag
    29881 ctctcaagtt tattgaattg acagaagaag gggaagttct agctgaacac ctctttgaag
    29941 caggggcaga caatccaatg gcccaacctc aagcctggca ccgagtggaa gctgccacag
    30001 atgatgtgga atggtacttg gaattttatt gtaaacctga ggattatttt gctaaaaaat
    30061 acaataccaa tcctgttcat tcagaggtcc tagaggccat gcagacagtg aaacaaggga
    30121 aagctttgga tttgggttgt ggtcaggggc gtaattctct ttttctagcc cagcaagatt
    30181 ttgatgtgac ggctgtagat caaaatggac tagctcttga aatcttgcaa agcattgtgg
    30241 agcaggaaga tttggacatg cctgttggcc tttacgatat caattcagct agcattgaac
    30301 aagaatatga ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg gaccgcattc
    30361 cagctattat tcaaaatatg caggagaaaa ccagtgttgg tggttacaac cttatcgttt
    30421 gtgccatgga cacggaggat tatccttgct cggttaactt cccattcacc tttaaagaag
    30481 gagaactggc agactattac aaggattggg aattggttaa gtacaatgaa aatccaggcc
    30541 atttgcaccg tcgcgatgag aatggcaatc gtattcaact acgctttgcg accttactag
    30601 ctaagaaaat caagtaaaca cacatgaaga ttaggaattt tcctgatctt ttttcttttt
    30661 tacgaatgat atagaaaagg agggaattca tgtttgttgc gagagatgct aggggagaat
    30721 tggtaaatgt gttagaggat aaacttgaga agcaagcata cacctgccca gcttgtggag
    30781 gccagctcca tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat aaatctttaa
    30841 aagactgtga ttttttcttt gaaaatgaaa gtccagaaca cctggccaat aaggaatccc
    30901 tctatcactg gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt tcagaactta
    30961 aacagattgc ggatgtattt gtaaatggca atctagctct agaagttcag tgtagtccct
    31021 tgcctcagaa agtccttaaa gagcgaagtg agggctatcg tagtcagggt taccaagtac
    31081 tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta cagcaaggtt
    31141 ttctttattt cagtcaaaac atgggctttt atgtttggga attagacaag ggaaaacaag
    31201 ttttaagact caaatacctg atttaccagg atctccgcgg taaactccat tatcaaatca
    31261 aggaattttc ctatggccaa ggtagtttat tggaaatatt gcgtcttccc tataagaaac
    31321 aaaaaatatc tcattttaca gtttctgagg acaaggacat ctgtcgctat atccggcaac
    31381 aactttatta tcaaaatctc ttttggatga aagaacaagc agaagcctat caaaagggag
    31441 aaaatatcct gacttatgga ctgaaagaat ggtatccaca aattcgacca atagtgggca
    31501 aatttttcca gattgaacaa gacttgacta gctattatca gcacttttat acctattacc
    31561 aaaaaaatcc tcaaaatgat tggcaaaagc tttatccacc agccttttat cagcaatatt
    31621 tcttgaaaaa tatggtagaa tagaaaggat ggaggaatct aatggtatta caaagaaatg
    31681 aaataaatga aaaagataca tgggatctat caacgatcta cccaactgac caggcttggg
    31741 aagaagcctt aaaagattta acagaacaat tggagacagt agcccagtat gaaggccatc
    31801 tcttggatag tgcggataac ctactagaaa tcactgaatt ttctcttgaa atggaacgcc
    31861 agatagagaa gctttacgtt tatgctcata tgaagaatga ccaggataca cgtgaagcta
    31921 agtatcaaga gtactatgcc aaggccatga cactctacag ccagttagac caagcctttt
    31981 cattctatga gcctgaattt atggagatta gcgaaaagca gtatgctgac tttttagaag
    32041 ctcaaccaaa gctgcaggtt tatcaacact attttgacaa gctcttgcaa ggcaaggatc
    32101 acgttctttc acaacgtgaa gaagaattat tggctggagc tggagaaatc tttggttcag
    32161 caagtgaaac cttcgctatc ttggacaatg cggatattgt gttcccttat gtcctagacg
    32221 atgatggtaa agaagttcag ctatctcatg ggacttacac acgtttgatg gagtctaaaa
    32281 aacgtgaggt tcgccgtggt gcctatcaag ctctttatgc gacttacgaa caattccaac
    32341 acacctatgc caaaaccttg caaaccaatg ttaaggtgca aaattaccgt gctaaagttc
    32401 gtaactacaa gagtgctcgt catgcagccc tcgcagcgaa ttttgttcca gaaagtgttt
    32461 atgacaattt ggtagcagca gttcgcaagc atttgccact cttacatcgc tatcttgagc
    32521 ttcgttcaaa aatcttgggg atttcagatc tcaagatgta cgatgtctac acaccgcttt
    32581 catctgttga atacaatttt acctaccaag aagccttgaa aaaagcagaa gatgctttgg
    32641 cagtcttggg tgaggattac ttgagccgtg tcaaacgtgc cttcagcgag cgttggattg
    32701 atgtttacga aaatcaaggc aagcgttcag gtgcctactc tggtggttct tacgatacca
    32761 atgcctttat gcttctcaac tggcaggaca atctggacaa tctctttact cttgttcatg
    32821 aaacaggtca cagtatgcat tcaagctata ctcgtgaaac tcagccttat gtttacggag
    32881 attactctat ctttttggct gagattgcct caactaccaa tgaaaatatc ttgacggaga
    32941 aattattgga agaagtggaa gacgacgcaa cacgctttgc tattctcaat aacttcctag
    33001 atggtttccg tggaacagtt ttccgccaaa ctcaatttgc tgagtttgaa cacgccattc
    33061 accaagcaga tcaaaatggg gaggtcttga caagcgattt cctaaataaa ctctacgcag
    33121 acttgaacca agagtattat ggtttgagta aggaagacaa tcctgaaatc caatacgagt
    33181 gggctcgcat tccacacttc tactataact actatgtata tcaatattca actggctttg
    33241 cggccgcctc agccttggct gaaaaaattg tccatggtag tcaagaagac cgtgaccgct
    33301 atatcgacta cctcaaggca ggtaagtcgg actatccact taatgtcatg agaaaagctg
    33361 gtgttgatat ggagaaggaa gactacctca acgatgcctt tgcagtcttt gaacgccgtt
    33421 taaatgagtt tgaagccctt gttgaaaaat taggattggc ataaaatggt tgaatcgtat
    33481 agtaagaatg ctaaccataa catgcgtcgt cctgtcgtca aagaagaaat tgtagacttg
    33541 atgcgtcagc gtcaaaagca ggtcacaggt ttcttgaaag aattggaaga ctttgcccgc
    33601 aaggaaaata ttcctattat tccccatgaa acggttgctt atttccgttt tcttatggaa
    33661 accatgcagc ctaaaaatat tctggaaatt gggacggcta tcggtttttc agctctcttg
    33721 atggctgaac atgcgccaaa tgctaagatt acaactattg atcgtaatcc agaaatgatt
    33781 ggttttgcca aggaaaattt tgcccagttt gacagtcgca agcaaatcac tctcctagag
    33841 ggagatgcgg ttgatgtctt atctacactg acagagtctt atgatttcgt ctttatggat
    33901 tctgccaagt ctaaatacat cgtctttctg ccagaaatcc tcaaacattt ggaagttggt
    33961 ggtgtggttg tcttggatga tatttttcaa ggtggtgatg ttgccaagga tattatggaa
    34021 gtccgtcgtg gtcagcgaac catttatcga ggccttcaaa aattatttga tgcaacctta
    34081 gacaatccag aactcaccgc aacattagtg cctttaggag atggtattct catgcttcgt
    34141 aaaaatgtag cagatgttca actgtctgaa agcgaatgat tttcagaaaa atttaagaaa
    34201 aaatagtaaa atagatagag taacacttat ctcaaaggag tagacatgaa gaaaaaatta
    34261 ttggcaggtg ccatcacact attatcagta gcaactttag cagcttgttc gaaagggtca
    34321 gaaggggcag accttatcag catgaaaggg gatgtcatca cagaacatca attttatgag
    34381 caagtgaaaa acaacccttc agcccaacaa gtcttgttaa atatgaccat ccaaaaagtt
    34441 tttgaaaaac aatatggctc agagcttgat gataaagagg ttgatgatac tattgccgaa
    34501 gaaaaaaaac aatatggcga aaactaccaa cgtgtcttgt cacaagcagg tatgactctt
    34561 gaaacacgta aagctcaaat tcgtacaagt aaattagttg agttggcagt taagaaggta
    34621 gcagaagctg aattgacaga tgaagcctat aagaaagcct ttgatgagta cactccagat
    34681 gtaacggctc aaatcatccg tcttaataat gaagataagg ccaaagaagt tctcgaaaaa
    34741 gccaaggcag aaggtgctga ttttgctcaa ttagccaaag ataattcaac tgatgaaaaa
    34801 acaaaagaaa atggtggaga aattaccttt gattctgctt caacagaagt acctgagcaa
    34861 gtcaaaaaag ccgctttcgc tttagatgtg gatggtgttt ctgatgtgat tacagcaact
    34921 ggcacacaag cctacagtag ccaatattac attgtaaaac tcactaagaa aacagaaaaa
    34981 tcatctaata ttgatgacta caaagaaaaa ttaaaaactg ttatcttgac tcaaaaacaa
    35041 aatgattcaa catttgttca aagcattatc ggaaaagaat tgcaagcagc caatatcaag
    35101 gttaaggacc aagccttcca aaatatcttt acccaatata tcggtggtgg agattcaagc
    35161 tcaagcagta gtacatcaaa cgaatagtcc aaatcaatga gtcagggaaa aaactcgact
    35221 tcaggaaaaa atgaagcaaa cattcccaca ataaaacgca tagtacaagg tttgtactgc
    35281 cccccaaaaa agttagacaa ttaatttatc cgaaggattt agttct
//
LOCUS       DAVMEI010000005        35308 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVMEI010000005 DAVMEI010000000
VERSION     DAVMEI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.005"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            70..366
                     /locus_tag="VYQ24_000212"
     CDS             70..366
                     /locus_tag="VYQ24_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1967402.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            443..2473
                     /locus_tag="VYQ24_000213"
     CDS             443..2473
                     /locus_tag="VYQ24_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1967403.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQGLFTDYL
                     QNFSSESVEVTSQKYYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDCLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            2475..3116
                     /locus_tag="VYQ24_000214"
     CDS             2475..3116
                     /locus_tag="VYQ24_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967404.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            3109..3402
                     /locus_tag="VYQ24_000215"
     CDS             3109..3402
                     /locus_tag="VYQ24_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967405.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            3653..4525
                     /gene="rsmA"
                     /locus_tag="VYQ24_000216"
     CDS             3653..4525
                     /gene="rsmA"
                     /locus_tag="VYQ24_000216"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1967406.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLMPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            4527..5405
                     /gene="rsgA"
                     /locus_tag="VYQ24_000217"
     CDS             4527..5405
                     /gene="rsgA"
                     /locus_tag="VYQ24_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1967407.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            5416..6072
                     /locus_tag="VYQ24_000218"
     CDS             5416..6072
                     /locus_tag="VYQ24_000218"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1967408.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            6035..6697
                     /locus_tag="VYQ24_000219"
     CDS             6035..6697
                     /locus_tag="VYQ24_000219"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1967409.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            6699..7955
                     /locus_tag="VYQ24_000220"
     CDS             6699..7955
                     /locus_tag="VYQ24_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1967410.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLYFQENEEEYED"
     gene            7945..8886
                     /locus_tag="VYQ24_000221"
     CDS             7945..8886
                     /locus_tag="VYQ24_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1967411.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            9153..9980
                     /gene="purR"
                     /locus_tag="VYQ24_000222"
     CDS             9153..9980
                     /gene="purR"
                     /locus_tag="VYQ24_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1967412.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            10047..11297
                     /locus_tag="VYQ24_000223"
     CDS             10047..11297
                     /locus_tag="VYQ24_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1967413.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            11423..12217
                     /gene="pflA"
                     /locus_tag="VYQ24_000224"
     CDS             11423..12217
                     /gene="pflA"
                     /locus_tag="VYQ24_000224"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1967414.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(12292..13218)
                     /gene="yidC"
                     /locus_tag="VYQ24_000225"
     CDS             complement(12292..13218)
                     /gene="yidC"
                     /locus_tag="VYQ24_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1967415.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(13296..13574)
                     /locus_tag="VYQ24_000226"
     CDS             complement(13296..13574)
                     /locus_tag="VYQ24_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1967416.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            13608..14348
                     /locus_tag="VYQ24_000227"
     CDS             13608..14348
                     /locus_tag="VYQ24_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1967417.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            14404..15087
                     /locus_tag="VYQ24_000228"
     CDS             14404..15087
                     /locus_tag="VYQ24_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1967418.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            15199..16191
                     /locus_tag="VYQ24_000229"
     CDS             15199..16191
                     /locus_tag="VYQ24_000229"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1967419.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            16256..16795
                     /gene="rsmD"
                     /locus_tag="VYQ24_000230"
     CDS             16256..16795
                     /gene="rsmD"
                     /locus_tag="VYQ24_000230"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1967420.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            16785..17273
                     /gene="coaD"
                     /locus_tag="VYQ24_000231"
     CDS             16785..17273
                     /gene="coaD"
                     /locus_tag="VYQ24_000231"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1967421.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            17257..18294
                     /locus_tag="VYQ24_000232"
     CDS             17257..18294
                     /locus_tag="VYQ24_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1967422.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            18352..18582
                     /locus_tag="VYQ24_000233"
     CDS             18352..18582
                     /locus_tag="VYQ24_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1967423.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            18606..19889
                     /gene="murA"
                     /locus_tag="VYQ24_000234"
     CDS             18606..19889
                     /gene="murA"
                     /locus_tag="VYQ24_000234"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1967424.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            19882..20070
                     /locus_tag="VYQ24_000235"
     CDS             19882..20070
                     /locus_tag="VYQ24_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1967425.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            20109..20933
                     /gene="endA"
                     /locus_tag="VYQ24_000236"
     CDS             20109..20933
                     /gene="endA"
                     /locus_tag="VYQ24_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1967426.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            21224..22555
                     /locus_tag="VYQ24_000237"
     CDS             21224..22555
                     /locus_tag="VYQ24_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1967427.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELISQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(22753..22823)
                     /locus_tag="VYQ24_000238"
     tRNA            complement(22753..22823)
                     /locus_tag="VYQ24_000238"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22789..22791),aa:Cys,seq:gca)
     gene            23239..26850
                     /gene="rpoB"
                     /locus_tag="VYQ24_000239"
     CDS             23239..26850
                     /gene="rpoB"
                     /locus_tag="VYQ24_000239"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1967428.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            26883..30560
                     /gene="rpoC"
                     /locus_tag="VYQ24_000240"
     CDS             26883..30560
                     /gene="rpoC"
                     /locus_tag="VYQ24_000240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1967429.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            30671..31084
                     /gene="ndk"
                     /locus_tag="VYQ24_000241"
     CDS             30671..31084
                     /gene="ndk"
                     /locus_tag="VYQ24_000241"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1967430.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(32013..32645)
                     /locus_tag="VYQ24_000242"
     CDS             complement(32013..32645)
                     /locus_tag="VYQ24_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967431.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(32647..34662)
                     /locus_tag="VYQ24_000243"
     CDS             complement(32647..34662)
                     /locus_tag="VYQ24_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1967432.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            complement(35112..35282)
                     /locus_tag="VYQ24_000244"
     CDS             complement(35112..35282)
                     /locus_tag="VYQ24_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967433.1"
                     /translation="MKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQLEIRVQSQ
                     KKSNGLDVGKAD"
BASE COUNT        10289 a         6939 c         8017 g        10063 t
ORIGIN      
        1 caacgttgat aatggaaaga ggagcaagta actcaacaat cttgtaatca aaaatagagg
       61 agtatcaata tgaaacaaac agttaaaaag ttagctcttg tagcgagcat tgcagcaaca
      121 ttaggtggag gtgtatcagt tgcctctgca gccgttcaat acccagaagg tggtgtttgg
      181 acttatggtt caggtaacgg tggtgcttac tcaaactact atcacccttc aaaataccat
      241 agttcaacag ttgttagcag aaaaactggt tcatctgaca agggatatgc tggtgctgga
      301 gggacttctc gtgcatggat tcgtacttct tggggagaga aagttgcatt ctactataat
      361 gtttagaatg gtatagccct attgtttagc tttacttggt caccttctca ctaagtgacc
      421 aagtaacttt ttggaggaaa tgatgaaacg tttatttatt ttgatttcaa tggtattggt
      481 atcgctttat atggtgataa cttccgttga ccatcgagaa gagattttat ttggtaacta
      541 tccttctgtt gatgtgacag gaatgatgat aaatcaacca gtagctagtc gcgaagaggt
      601 gacagaggct ttgagtcact tggcggtaga gcataatagt ctcatcgccc gtcgaatcgt
      661 tgagccaaat gaagctggag aaacactctt tacctatgcc acttatggtg agggagagct
      721 tccagaaggt ctgaccattt cctccaagga gagtgcagaa acgagtgatt tattagggtc
      781 ttacttgatt gtgtcaggaa gtttggatgg agtgagctta cagaccacct tgaaagaact
      841 tggctatcaa ggctttgttt cgaatggaga agatccattt tcgatagtct tactattgac
      901 ggccacccct atggtgctac tgagtttagc tatttttctg ctgaccttta tgagtctgac
      961 cctgatttat cggatcaaat cccttcgtca ggcagggatt cgcttaatag ctggtgagag
     1021 cttgtttgga gttgctctca gaccagtgtt agaagatgtg agacagctta tctgctcagt
     1081 gctggtatcc agtcttttgg gattggggat tctctggtat caaggtgcct tgtttatggc
     1141 aacggtgcaa ctggtcatca ttgctcttct actttatgga ttgaccctgg cagggatttc
     1201 taccttacta agtgtcgtct atctacttgg tttacaggaa aatagtctgg tggatctatt
     1261 gaaagggaaa ctccctctca aacgtatgat gacattgatg atggtggggc aactcttagc
     1321 tgtattggtg gtcggatcga gtgcgacagc tctcctaccc cactaccgtg aaatgcagga
     1381 aatggagaga gctagcaata aatggagcca gtcctcagac cgttaccgtc tatcctttgg
     1441 ttggtctagt gcatttgccg atgaagaagg aacgcgtaag gataatcgtg agtggcagac
     1501 atttactgaa gaacggttag ccaatacaga ctctttttat attatgagca atgttgacaa
     1561 tttctcagat ggagcagaag tggacctaga tggcaatcgt ctcagtgact acacaccgtc
     1621 agggaatgtt atctatgtct caccacgcta tctgatagaa gaaaagatta ccgtttcttc
     1681 agagtttatg gacaagatgc aaaacttgtc tgagggagag tttgggctga tcttgcctga
     1741 gagcttgcga gagcagtcta cctactacca aggattgttt acagattacc tgcaaaactt
     1801 ttcatctgaa agtgtagaag tgacgagtca gaaatactac ctcccacagg taaggctagc
     1861 ttttacagaa acaggacagg aacgtttcct ctataatgat gggtacaaga caacacgcca
     1921 gtacctaaaa gatccgatta ttgtagttct aacgccgcaa gcgactggaa caagacctgt
     1981 tgcagggatg ttgtggggaa ctacggctaa tagtgccttg aaactagatc gatatggaga
     2041 cagcatcaca gctctaaaag agcaaggtct gtatcacaag gtttcttact tggtaaaaag
     2101 ccagctattt tttgccaagg tactaaatga caaacgggtg gagttttact ctctccttat
     2161 tgggacgatt ttgaccctgt ctacggctat cttgttattt gattccatga atcttctcta
     2221 ttttgagcag ttcagacggg aacttatgat taaacgtctt gctggtatga caatctatga
     2281 gcttcatggc aagtatttac tggcgcaagg aggagttctc ttgcttggcc tagtcctatc
     2341 tagtattttg acaagagatt gtttgattag cgctctagtt gtagctttgt ttacgcttaa
     2401 cgccctcttg attttagtaa ggcaggacaa aaaagaagaa gctggtagca tggcagtatt
     2461 gaaaggaaaa taagatgatt gatattcaag gattggaaaa gaaatttaat gaccgcgcga
     2521 ttttctctgg tttgaatctc aagctggaga agggcaaggt ttatgcctta atcggaaaga
     2581 gtggaagcgg aaagacgacg ctgctgaata tcttgggaaa gctagaaaag atagatggtg
     2641 gaagggttct ctatcagggg aaagatttaa aaaccattcc cactcgtgag tattttcgag
     2701 accagatggg ctatctcttt caaaatttcg gcctcttaga aaaccaatca atcaaagaaa
     2761 atttggattt gggttttgtt ggtcagaaaa tctcaaaagt agaacgtttg gaaaggcaag
     2821 tgggggcttt agaaaaagtt aatctagggt atttggattt agaacaaaaa atctatactt
     2881 tatctggggg agaggcccaa cgagttgccc ttgctaagac tattttgaaa aatccaccct
     2941 tgattttggc agatgaacca acagcagctc ttgatcctga aaattcagag gaggttatga
     3001 atctcttggt ggatttgaaa gatgaaaatc gaattatcat cattgcgacc cataatcccc
     3061 tagtctggaa taaggctgat gaaatcattg atatgaggaa acttgctcat gtgtgaaaaa
     3121 atccgtattc gcagggtatc tgattatcct agtgccagag gtggtttaga agatatcctc
     3181 atcatggaaa atatgaccaa tcatctcctt ttggttcaaa tccgagtgca tggctatttg
     3241 cttgattttg ctagtattga agggcaaagg caaaagcatt atcgtttgaa aaatttacct
     3301 cagacggttg aactgacagt ggatgatgtg gaggaggatg tggatttgac cctacccgaa
     3361 aatcgaagtt atcaagaagc tgattttttt gatactcaat gaaaatcaaa gagcaaacta
     3421 ggaagctagc cgcaggctgt acttgagtac ggcaaggtga agctgacgtg gtttgaagag
     3481 attttcgaag agtatgaacg tatgtttcga gagaagcact aagtccctct ttaaagattt
     3541 ccaagactat ctttcttcat gaggaaagat agttttttgg tatgattttc attcccaaaa
     3601 tacaagggga atgtgttaca atagtagtaa cagataatag aaaagagaat agatgagaat
     3661 tgcagattat agcgtgacca aggcagtgtt ggagcgtcac ggttttacct ttaaaaagtc
     3721 ctttgggcaa aatttcttga cggataccaa tatccttcaa aaaattgtgg atacggctga
     3781 aattgatgat caggtcaatg tcatcgaaat cgggccaggt attggtgcct tgacagaatt
     3841 tttggctgag cgtgcagccc aagtcatggc ttttgagatt gaccaccgtt tgatgccaat
     3901 tttggcagat actctgcgtg attttgataa tgtgaccgta gttaacgaag atattctcaa
     3961 ggttgatttg gcgcaacata tccaaaattt taaaaatcct aacctgccaa tcaaggtagt
     4021 ggctaatttg ccttactaca tcacgacgcc tattctcatg cacttgattg agagtggcat
     4081 tcctttttgt gagtttgtgg tcatgatgca gaaagaagta gcggaccgca tttcagccca
     4141 gcctaacacc aaggcttacg gtagcttgtc tatcgccgtg cagtattaca tgacagccaa
     4201 ggttgccttt atcgtgcctc gtacggtctt tgtgccagcg ccaaatgtgg attcagccat
     4261 cttgaaaatg gtgcgtcgtc cagagccagc cgtagcagta gaagatgaga actttttctt
     4321 taaggtttcc aaggctagtt ttacccatcg ccgcaagacc ttgtggaata acttgacagg
     4381 ttactttggt aagactgaag aggtcaagga caagctgacc aaggctttgg accaggcagg
     4441 cttgtcacca agtgtgcgtg gggaagctct cagcttggca gaatttgccg gtctagcaga
     4501 cgcacttaaa gggcaaggac tctaagatgc agggacaaat cattaaagcc ttggcaggtt
     4561 tctactatgt ggagagtgat ggccaggttt atcaaacacg cgcgcgtggg aatttccgta
     4621 aaaaaggcca taccccttat gttggggact gggtagattt ctctgccgag gaaaattcag
     4681 aaggctatat cctcaaaatt cacgaacgga aaaacagtct ggttcgtccg cctattgtca
     4741 atatcgatca agctgtagta atcatgtccg tcaaggaacc tgattttaac agcaatttgc
     4801 tggatcgttt cttggttctt ttggagcaca agggcatcca tcccattgtc tatatttcca
     4861 aaatggattt gttggaagat aggggagaac tggattttta ccagcagacc tatggtgaca
     4921 tcggctatga ctttgtgacc agtaaagagg aactcctgtc tttgttaaca ggcaaggtta
     4981 cggtctttat ggggcagaca ggtgttggga agtcaactct tctcaataaa atcgcaccag
     5041 acctcaatct tgaaacggga gaaatttcag acagtctagg tcgcggtcgc cataccactc
     5101 gagctgttag tttttacaat ctcaacgggg gtaaaatcgc agatacacca ggcttttcat
     5161 ctctggacta tgaagtatca agggctgaag acctcaatca ggcctttcca gagattgcta
     5221 ctgttagccg agattgtaag ttccgtactt gtacccatac ccatgagccg tcttgtgccg
     5281 tcaaaccagc tgttgaagag ggtgttattg caaccttccg ttttgacaat tacctgcaat
     5341 tccttagtga aattgaaaat cgtagagaaa cctataaaaa agtcagcaaa aaaattccaa
     5401 aataaggaga aacctatgtc tcaatacaag attgctccgt caattctggc agcagattat
     5461 gccaactttg aacgtgaaat caaacgtcta gaagcaactg gggcagaata tgcccatatc
     5521 gatatcatgg acagtcattt tgtaccgcaa atcagttttg gtgcaggtgt ggtcgagagc
     5581 cttcgtcctc atagtaagat ggttttcgat tgccacttga tggtgtcaaa ccctgagcat
     5641 catctggaag attttgcgcg tgcaggtgca gacatcatca gtatccatgt agaagcaacg
     5701 cctcatattc atggcgccct ccaaaaaatt cgttcactcg gagttaagcc ttcagtcgtt
     5761 atcaatcctg gcacaccagt tgaagccatc aagcacgtcc ttcatctagt tgaccaagtt
     5821 ttagtcatga cggttaatcc aggttttggt gggcaagcct ttctgccaga aaccatggat
     5881 aaggttcgtg agttggttgc tcttcgtgag gaaaaaggtt tgaactttga aatcgaagtg
     5941 gatggtggga ttgatgacca aactattgct caagccaaag aagctggtgc gactgttttt
     6001 gtagcaggtt cctatgtctt taagggagaa gtcaatgagc gagtacaaac tctcagaaaa
     6061 caactggact agggttgcag tttttgcagg cggaaaccgc ggtcattatc ggacagattt
     6121 tgatgctttt gttggggtgg atcgaggctc gctctgggtc ttggaagaag acttacctct
     6181 tgctctagca gtcggagatt ttgattctgt gacggaagaa gagcgacagg tgattcaaaa
     6241 acgtgcccag tattttgtcc aagcccgacc agaaaaagat gataccgatt tggaattggc
     6301 tctcttaacc atctttgaga aaaatcctca ggctcaggtt actattttcg gtgccttggg
     6361 tggccgtatt gaccatatgt tggccaatgt ctttctgcct agcaatccta agttggcacc
     6421 ctatatgcat caaatagaaa ttgaggatgg gcaaaacttg attacttatt gtccagaagg
     6481 gaccagtcag ctagaacctc gttcagacta cgactatcta gcctttatgc cagttcggga
     6541 tagccagctg actattcttg gagccaagta tgagttgaca gaggaaaatt ttttctttaa
     6601 aaaagtgtac gcttctaacg aatatataga tagggaagtg tcggtaactt gcccagatgg
     6661 ttatgtggtc gtactgcata gcaaggatag gaggtaggat ggaaagttta cttattctat
     6721 tattaattgc caacctagct ggtctctttc tgatttggca aaggcaggat aggcaggaga
     6781 aacacttaag taagagcttg gaggatcagg cagatcattt gtcagaccag ttggattacc
     6841 gctttgacca agccagacaa gccagccagt tagaccaaaa agatttggaa gtggctgtca
     6901 gcgaccgttt gcaagaagtg cggattgaat tgcaccaagg cctgacccaa gtccgtcaag
     6961 aaatgacaga taatctcctc caaactagag ataagacaga ccaacgtctc caagccttgc
     7021 aggaatcaaa tgagcaacgt ttggaacaaa tgcgccagac ggtcgaggaa aaactagaaa
     7081 agaccttgca gacacgctta caggcttcct ttgagacagt ttctaaacaa ctggagtctg
     7141 tcaatcgtgg tcttggagaa atgcagacag ttgcccgtga tgtcggagct cttaacaagg
     7201 ttctctctgg aaccaagacg cgagggattc tgggagaatt gcaactgggg caaattattg
     7261 aagacatcat gacacctgcc cagtacgaac gagaatacgc aacggttgaa aactctagtg
     7321 aacgagtgga gtatgccatc aagttacccg gacaaggcga ccaagaatac gtctatcttc
     7381 caattgactc taagtttcca ctggcagatt attaccgctt ggaagaagcc tatgagacag
     7441 gtgacaagga tgagattgaa cgctgtcgta agtcactcct agcaagcgtc aagcgctttg
     7501 ccaaggatat caagagcaag tacatagcac cgcctcggac aaccaacttt ggaatcttgt
     7561 ttgttccgac agaaggtctt tattcagaga ttgtgcgcaa tccggtcttc tttgatgatt
     7621 tgagacggga agaacagatt attgttgcag gaccaagtac cctatcagcc cttcttaact
     7681 ccctatcagt tggtttcaag acccttaata tccaaaagag tgccgaccat atcagcaaga
     7741 ctcttgccag tgtcaagacc gagtttggca agtttggtgg tattctggtc aaggcacaaa
     7801 aacatctcca acatgcctct ggcaatattg atgaattatt aaaccgtcgt accatagcta
     7861 tcgagcggac gctccgtcac attgagttgt cagaaggtga gcctgcgctt gatctactct
     7921 attttcaaga aaatgaggaa gaatatgaag attagtcaca tgaaaaaaga tgagttattt
     7981 gaaggctttt acctaatcaa atcagctgac ctgaggcaaa ctcgagctgg gaaaaactac
     8041 ctagccttta ccttccaaga tgatagtggc gagattgatg ggaagctctg ggatgcccaa
     8101 cctcataaca ttgaggcctt taccgcaggt aaggttgtcc acatgaaagg acgccgagaa
     8161 gtttataaca atacccctca agtcaatcaa attactctcc gcctgcctca agctggtgaa
     8221 cccaatgacc cagctgattt caaggtcaag tcaccagttg atgtcaagga aattcgtgac
     8281 tacatgtcgc aaatgatttt caaaattgaa aatcctgtct ggcaacggat tgtccgaaat
     8341 ctctacacca agtatgataa ggaattctac tcctatccag ccgccaagac caaccaccat
     8401 gcctttgaaa cgggcttggc ttatcatacg gcgaccatgg tacgtttggc agacgctatt
     8461 agcgaagttt atcctcagct caataagagc ttactctatg cggggattat gctgcatgac
     8521 ttagctaagg ttatcgagtt gacggggcca gaccagacag agtacacagt gcgaggtaat
     8581 cttcttggac atatcgctct cattgatagc gaaattacca agacagttat ggaactcggc
     8641 atcgatgata ccaaggaaga agtcgttttg cttcgtcatg tcatcctcag tcaccacggc
     8701 ttgcttgaat atggaagtcc agtccgtcca cgtattatgg aggcagagat tatccatatg
     8761 attgacaatc tggatgcaag catgatgatg atgtcaacag ctctggcttt ggtggataaa
     8821 ggagagatga ccaataaaat cttcgctatg gataatcgtt ccttctataa accagattta
     8881 gattgataat ttaagaaaaa tgagcatttt ttaggataag aatgttcgtt tttttatgtg
     8941 aatatggtat aataagtaaa agacaaaaat gaatactctt cgaaaatctc ttcaaactag
     9001 ggtagtatcg ccttgtcgta tgtatatatg caggtatatt acagggtttg tcagttctat
     9061 cgacaatctc aaagcagtgt tttgaaccac cagcgaccag ctttctagtt tgctttttga
     9121 ttttttgaat aaaaatggaa taggaaatag aaatgaaatt aagaagaagt gatcggatgg
     9181 ttgtcatttc caactatttg attaataatc cttataaact aactagtctc aatacttttg
     9241 ctgaaaagta tgagtctgct aaatcatcca tctcagaaga tatcgtcatt atcaaacgcg
     9301 cctttgagga aattgaaatc ggtcatatcc agacagtgac tggggctggc ggaggtgtca
     9361 tcttcacacc gtctatttcg agtcaggatg ctaaggaaat ggttgaagac ttgcgtacca
     9421 agttgtcaga aagtgaccgt atcttgccag gtggttatat ctatctgtct gatttgctta
     9481 gcacaccagc catcttgaaa aatattggtc gtattattgc caaaagcttt atggaccaaa
     9541 aaattgacgc ggttatgacc gtagcaacta agggtgtgcc acttgcaaat gcagttgcca
     9601 atgtcctcaa tgtctctttt gtcattgtgc gccgtgacct gaaaattacc gaaggttcaa
     9661 ctgttagcgt caactatgtt tcaggttcaa gtggtgaccg tatcgagaaa atgttccttt
     9721 caaaacgtag tcttaaggca ggtagccgtg tcttgattgt ggatgacttc ttgaaaggtg
     9781 gcggaacggt caacggtatg attagtctct tgcgcgagtt cgactcagaa ctggcaggtg
     9841 tagcggtctt tgcggacaat gcccaagaag aacgtgaaaa gcagtttgac tacaagtcac
     9901 tcttgaaggt aactaatatt gatgtcaaga accaagccat cgatgttgag gttggcaata
     9961 tctttgacga agataaataa gagatagaac taaaggttgg aacgattgtc ccagcctttc
    10021 tttgcaaaca gaatagaagg aagcttatga aaacaccatt tatcaataga gaagagttag
    10081 aagcgattgt tgccgagttc ccgactccct ttcacttgta tgatgagaag gggattcgtg
    10141 agaaggcaag agccgtcaac caagcttttt cgtggaacaa gggctttaag gaatattttg
    10201 cagttaaggc tactccaact ccagctattt tgaaaattct ccaagaagaa ggttgtggtg
    10261 tggactgctc tagttatgta gagcttttga tgagccataa actggacttt ctgggttctg
    10321 agattatgtt ctcttccaac aacacgccag acaaggaata cgcctatgca cgtgaattgg
    10381 gtgcgaccat taacttggat gcctttgaag atattgaaca tctggagaga gtagcaggca
    10441 ttccagaaat catctcttgt cgttataatc ctggaggcgt ttttgaactg gggacagaca
    10501 ttatggacaa tcctggggag gctaagtttg gcatgaccaa ggaccagctc tttgaagcct
    10561 ttgctatctt gaaggaaaaa ggagccaaga cttttgggat tcactccttc ctagcgtcca
    10621 ataccgtgac ccatctctat tatccagagt tggctcgtca gctctttgaa ctggctgttg
    10681 aaatcaagga aaagttgggc atttggctag actttatcaa tctttctggc ggtattggtg
    10741 ttaattatcg tccagaccag gagccgaacg atatcgcctt gattggtgag ggagttcgta
    10801 aggtgtatga agaagttctt acgtcagcag gtcttggtca ggtcaagatt ttcaccgaat
    10861 tgggtcgttt tatgctggca cctcacggtg ctctagtcac aagagtgact cataagaagg
    10921 aaacctaccg tacctatcta ggtgtggatg cctcagcagt caacctcatg cgtccagcta
    10981 tgtacggagc ttaccatcat attagcaacg tgacccatcc agatggacca gctgaagcgg
    11041 tagatgtggt tggttcactc tgtgaaaaca atgataaatt tgcagtcaat cgcgaactgc
    11101 ctcatacaga aatcggtgat ttgctggtaa ttcatgatac aggtgcccac ggattttcaa
    11161 tgggctacca gtataatgcc aaattacgtt ctgcggaaat cctctatacc gaagaaggta
    11221 aagcccgtca aatccgccgt gcagagcgcc ctgaggacta ttttgcaacc ttatatggct
    11281 tcgattttga agaataatct gataatagat tgaaaatgaa attgaaaaac agattgcttt
    11341 ctaaaaaata ggcaaaaatc ttgtttttcc ttcaagtcgt gatataataa aactataaaa
    11401 cgttttcaag gaaggtaacg atatgtctga agaaacaatt gattatggac aagtgacagg
    11461 aatggtgcat tcgacagaaa gctttgggtc agtagatggg cctggtattc gctttattgt
    11521 ctttttgcag ggctgtcaca tgcgttgcca gtattgccac aacccagaca cttgggctat
    11581 ggagtccaat aagtcacgtg aacggacggt agatgatgtc ttgacagagg ccttgcgcta
    11641 ccgtggtttc tggggaaata agggtgggat tacagtcagt ggaggagaag ctctcttgca
    11701 gattgatttc ctgattgctc tcttcaccaa ggctaaggaa caaggaatcc actgtacctt
    11761 ggatacctgt gctcttcctt tccgtaataa accacgttac cttgagaagt ttgacaaact
    11821 catggctgtc actgacttgg ttcttttgga tatcaaggaa atcaacgaag aacagcacaa
    11881 gattgtcact agacaaacca ataaaaatat cttggcttgt gcccagtatc tatcagatat
    11941 tggaaaacct gtctggattc gccacgtgct agttccagga ttgacagaca gagatgatga
    12001 cttgattgaa cttggtaagt tcgtcaagac cctcaaaaat gttgataagt ttgaaattct
    12061 accttatcac accatgggtg agttcaagtg gcgtgaactt ggaattccat attccctcga
    12121 aggagtcaaa ccaccaacag cagatcgcgt caagaacgct aaaaaactca tggataccga
    12181 aagttatcaa gattatatga aacgtgtaca tggatagaaa agaagcctga tggaaacatc
    12241 gggcttttga tttgcaaaaa gacttagcaa atcagctaag cctttttctt cttatctcga
    12301 acgttgtttt ccagcgttgc gatttttgtg ttttttcttg cttgtgatag cagttggttg
    12361 ttcaggggta acgtcttttc gtccacttgg tttagagaaa gcacttgctt ttggtgggtt
    12421 cttggctagt tcttcacgga cttttttgcg aagttttgga cgaacgatat agttgacgat
    12481 aaactgttgg agaatcatca tgaaaccacc gacaacccag taaagtgtga cactagctgg
    12541 tgagaagagg gagaagacga cgatcatgag tgggctcatg taaatcattt tcttgatttg
    12601 ttctctttgc atttcatctt ctactccgtg aagtgaaagg agcgattgaa gatagtaaag
    12661 gacaccagca caggcaacca aaatcatact tggagaacct agaggaatgc ctaggtagct
    12721 tgcttgagca accccttcag tatgttgggc agcaaagtag atagcagaga agaaaggcat
    12781 ttgaaggagg atagggaaac atcctacacc gccaaacatg ctgataccgt gctctttttg
    12841 agcagcaaag agagcttgtt gggcttcgag tttttcttct tgagtagtcg cttctttgag
    12901 acgcgtttgg tgtggctcaa ggacgtgctt gagggcgttc atcttttcag agtgaagcgt
    12961 tgccttccat gattggtaga taccaagtgg caagataatc aagcgcacga taatggttac
    13021 gatgatgata gcgacaccaa agcctagacc tttatcagta gcgaagtact tgatagcttc
    13081 agccataggg gctccgatgg tattccaaat aaatcctgtt ggctgacctg tggttttatc
    13141 gacattgaca caaccagtca agacaagcaa catagccgct cccatagctg agagtgcaaa
    13201 acgtttaata gatttcactg tttttattcc ttctttaaaa attatacctt tctattctac
    13261 tgttttttta caaaatatac aatagttctg gagacctaat ttgcgatttt aaagtcagag
    13321 tagggaggaa agttgcttag tttgacatct aagtagctta cttttgaaaa aggtgtcggt
    13381 ccttttcgga tttcttggat aaattttgcc atgatagcag atgagtctgc ttgggctaag
    13441 atttccactg tgccatcgtc gttattccat actcgacctg tgatgccacc aatttcaagt
    13501 gccaagctgt aaacacccca acgaaagccg actccctgta ccctaccttg ggcaatcatt
    13561 ctaacctttt gcataccaaa cccctttgat tgtgttataa tatttctatg actattataa
    13621 cctcaaaagc caattctgtg gtaaaaaatg ccaagaaatt acgccaaaaa aaataccgca
    13681 agtctgccta cttgattgaa ggctggcact tgtttgaaga agctgtccaa gctggagtga
    13741 cgattgagaa aatctttgcc ctagaaaatt atcgagatca gttagctact ttttcgcaaa
    13801 ctgtctgggt gtcagaggat attttgctag atttggcgga ttctcagact ccacagggaa
    13861 ttgttgccgt ggttcaaaaa gaagaagtag gacaagctga tttgagtcag ggcaagttct
    13921 tgtttttgga agatgtgcaa gatcctggta atgtaggaac tatcattcga actgcggatg
    13981 cagcaggttt tacaggagtg attgtttcag ataagtcggc agacatctac agtctcaaaa
    14041 ccctacgttc catgcaaggt agtcatttcc atctgcccat ttaccggatg actagtcaag
    14101 cgcttcttga cgaaactaaa aaggtagcta tcccagtgct agcaacaacc ctatctaaag
    14161 attctgttga ttacagagaa ctgccttcta tagaaaattt tgtactagtt atgggaaatg
    14221 agggtcaagg aattagtccc cttatggctg aaagtgcaga ccagttggtc catattagca
    14281 tgaaggggca ggccgagagt ttgaatgttg cggttgcagc cggtatttta atcttccatt
    14341 taagctaatt ttaacttttt ttgttataat caaggaaaga tgttcacaga aaaggagaaa
    14401 tggatgaatc acactattat acatgaccgt gcaggtctca atcaatttta cgctaaggtt
    14461 tatgcctttg ttggtctggg aatcggacta tctgctttgg tatcaggcct tatgttgacg
    14521 gtctttcagt ctcagttggt ttactttttg atgcaggggc gtctctggtt gaccattgct
    14581 acttttgcgg agttggcttt ggttttcgtt gccagtagca tggcttcaag aaatagtcca
    14641 gctgctcttc cagtattttt actttactcc gttttaaacg gctttaccct tagttttgtg
    14701 gtggctttct atactccggg tacagtttta tctgcctttg tatcgagcgc ccttctcttc
    14761 tttgtcatgg cggcagttgg tatttttacc aagaaagact tgagtgggat tggtcgagca
    14821 atgatggcag ctctcattgg tttactgatt gctatggtag tcaatatttt cttggctagt
    14881 ggcttctttg attatatgat tagcgtagcc atggttttgg ttttttcagg gttgattgct
    14941 tgggataacc aaaagattcg cctcgcctat gaacaatcac aaggtcgtgt agcgacaggc
    15001 tgggtggtgt caatggctct cagcatctat cttgatttta tcaatctttt cctaagtatt
    15061 ctacgtatct ttggtcgcaa tgactaaaag gtctatgaaa tcagtgtttt gagtgacact
    15121 gatttttttt aaattttctc ttttcttttc ttggaaatag gtgtataata cgtttattaa
    15181 atttttgagg agttgtctat gaagaaaagt tttatccatc aacaagaaga aatttccttt
    15241 gtcaaaaaca cttttaccca gtatttgaaa gataagctag aagttgtcga agttcaaggt
    15301 cctatcttga gtaaggtcgg tgacggaatg caggacaacc tgtctggtgt ggaaaatcca
    15361 gtatcggtca aggttctcca aatccctgat gctacttatg aagtggtgca ctcacttgct
    15421 aaatggaaac gccacacctt ggctcgtttt ggctttggtg aaggagaggg tctctttgtc
    15481 cacatgaaag cccttcgtcc agatgaggat tccttggatg caacccactc tgtttatgtt
    15541 gaccagtggg actgggagaa ggttatccca aatggtaagc gtaacatcgt ttatctaaaa
    15601 gaaacagttg agaagattta taaggctatt cgcctgactg agctagctgt tgaagcccgc
    15661 tatgacatcg agtctatctt gccaaaacaa atcaccttta tccatacaga agaattggta
    15721 gaacgctacc cagacttgac acctaaagaa cgtgaaaatg cgatttgtaa agaatttgga
    15781 gccgtctttt tgattggtat cggtggcgag ttgccagatg gtaaaccgca cgatggacgt
    15841 gcaccagact atgatgactg gacaagcgag tctgagaatg gctacaaggg tctaaatggt
    15901 gatattcttg tctggaatga gtctttaggt ggagcctttg agttgtcttc tatgggaatt
    15961 cgtgtagatg aagaaactct tagacgtcag gtggaaatta caggtgatga agaccgcttg
    16021 gaattggaat ggcacaagtc tttgttgaat ggcctattcc cattgacaat cggtggagga
    16081 attggacaat ctcgtatggc catgttccta cttcgcaaga gacacatcgg agaagtgcaa
    16141 acaagtgttt ggcctcaaga agtccgcgat acttacgaaa atattttgta gagaatcgaa
    16201 ccgcaaggtt cggttttctt tctctttttg tctataattt ggtataataa acagtatgaa
    16261 aatcgtatca ggaatctatg ggggacgtcc cctcaagaca ctagaaggca agacaacaag
    16321 acctacttcg gataaggtta ggggagccat ttttaacatg attggtccct actttgaagg
    16381 gggacgagtc ttggaccttt atgcaggtag tggtggttta tctatcgaag cagtatcgcg
    16441 tggcatgtcc agtgctgttt tggtggagcg agaccgtaag gctcagacca tcgtggctga
    16501 aaatatccag atgaccaagg aagttggaaa atttcaactc ctcaagatgg atgcagagag
    16561 ggcattggaa caggtatctg gggaatttga cctcgttttc ttagaccctc cctatgccaa
    16621 ggaacaaatc gtagcagata ttgaaaaaat ggctaagaga gagctttttt ctgaagatgt
    16681 tatggttgtg tgcgagacgg ataaggccgt tgaacttcca gaagagattg cctgtctggg
    16741 tatctggaag gaaaagattt atggaattag taaggtgaca gtctatgtca gataagattg
    16801 gcttattcac aggctcattt gatccgatga caaatgggca tctggatatc attgaacggg
    16861 cgagcagact ttttgataag ctttatgtgg gtattttttt taatccccac aaacaaggat
    16921 ttctccctct tgaaaatcgt aaacgggggc tagaaaaggc tttgggacat ttggaaaatg
    16981 ttgaagtcgt ggcttctcat gatgaattgg tggtcgatgt tgcaaaaaga ctgggggcta
    17041 ctttcctagt acgggggttg aggaatgcgg cggatttgca atatgaagct agttttgatt
    17101 actacaatca tcagctgtct tctgatatag agactattta tttacatagc cggcctgaac
    17161 atctctatat cagttcatca ggtgttcgag agcttttgaa gtttggtcag gatattgcct
    17221 gccatgttcc cgagagtatt ttggaggaaa taagaaatga aaaaaaagat tagatggccc
    17281 ttatacgtca ttgcggcctt gattgtgact ttcttggcat ttgtagtgcc cttgccttat
    17341 tatatagagg ttccaggtgg ttcggaagat attcgccaag tccttaaagt aaatgacaca
    17401 gaagataagg aagctggtgc ctatcaattc gttacggttg gtgttcaaca tgccacttta
    17461 gctcatatga tttatgcttg gttgacacct tttacagata ttcgtagtgc tcaggagact
    17521 acaggtggtt cttccgatgt tgaatttatg cgaatcaatc aattctacat gcaaacatcg
    17581 caaaatatgg ccaagtatca aggactaaaa acagctggta aggatatcga actcaagtac
    17641 tttggagttt atgttttgaa tgtgacggat aattcaacct ttaaagggat tctcaatatc
    17701 tctgatacag tcacagcagt caatgatcag acctttgata gttccaaaga cttgattgat
    17761 tacgtcagtt ctcaaaaatt aggggattcc gtcaaggtca cctatgaaga ggatgggcaa
    17821 accaagtctg cagaaggaaa gattatcacc ttggaaaatg gcaaaaatgg aattggaatc
    17881 ggcttgattg accgtacaga ggtaatcagc aatgtcccaa ttagcttttc aacagctggt
    17941 attggcggcc caagtgctgg tctcatgttt agtctagcta tctatactca aatagctcac
    18001 ccagatcttc gtaatggtcg tattgttgcc ggtacaggta ccattgaccg cgatggtaat
    18061 gtgggagaca ttggaggtat tgataagaag gttgtagctt cggctagggc aggtgctgct
    18121 attttctttg ctcctgataa ccctgttagc gaagaagaac aaaaggcgca tctggacgcg
    18181 aaaaacaact accaaacagc cctagaagcg gctaaaacaa tcaagacgga tatgaaaatc
    18241 gtgcccgtta aaaccctaca agatgcgatt gattacttga aaaacaatcc ctaatttttg
    18301 agtgaagttt ccaaactagc gcacaaacag agaagatggt ataatagtca aatggttcaa
    18361 ttattattca ctctaagtag tcatatgctc tttatttatg tgagttttta ccttttaaag
    18421 gatcttgtta gatgggagaa ggttttaaaa gtgacagatg ataatacaag aaaagttcgt
    18481 ttattagtag ccttttttag cattgtcata ggctacatcc tgagttcttt ctttattagc
    18541 ctgtatcatt tgtggcaaga agcgcttaga ggattattat aaaatcaaga gtaaaggaaa
    18601 cgagtatgga taaaattgtg gttcaaggtg gcgataatcg tctggtagga agcgtgacga
    18661 tcgagggagc aaaaaatgca gtcttaccct tgttggcagc gactattcta gcaagtgaag
    18721 gaaagaccgt cttgcagaat gttccgattt tgtcggatgt ctttattatg aatcaggtag
    18781 ttggtggttt gaatgccaag gttgactttg atgaggaagc tcatcttgtc aaggtggatg
    18841 ctactggcga catcactgag gaagcccctt acaagtatgt cagcaagatg cgcgcctcca
    18901 tcgttgtatt agggccaatc cttgcccgtg tgggtcatgc caaggtatcc atgccaggtg
    18961 gttgtacgat tggtagccgt cctattgatc ttcatttgaa aggtctggaa gctatggggg
    19021 ttaagattag tcagacagct ggttacatcg aagccaaggc agaacgcttg catggcgctc
    19081 atatctatat ggactttcca agtgttggtg caacgcagaa cttgatgatg gcagcgactc
    19141 tggctgatgg ggtgacagtg attgagaatg ctgcgcgtga gcctgagatt gttgacttag
    19201 ccattctcct taatgaaatg ggagccaagg taaaaggtgc tggtacagag actataacca
    19261 ttactggtgt tgagaaactt catggtacga ctcacaatgt agtccaagac cgtatcgaag
    19321 caggaacctt tatggtagct gctgccatga ctggtggtga tgtcttgatt cgagacgctg
    19381 tctgggagca caaccgtccc ttgattgcca agttacttga aatgggtgtt gaagtaattg
    19441 aagaagacga aggaattcgt gttcgttctc aactagaaaa tctaaaagct gttcatgtga
    19501 aaaccttgcc ccacccagga tttccaacag atatgcaggc tcaatttaca gccttgatga
    19561 cagttgcaaa aggcgaatca accatggtgg agacagtttt cgaaaatcgt ttccaacacc
    19621 tagaagagat gcgccgcatg ggcttgcatt ctgagattat ccgtgataca gctcgtattg
    19681 ttggtggaca gcctttgcag ggagcagaag ttctttcaac tgaccttcgt gccagtgcag
    19741 ccttgatttt gacaggtttg gtagcacagg gagaaactgt ggtcggtaaa ttggttcact
    19801 tggatagagg ttactacggt ttccatgaga agttggcgca gctaggtgct aagattcagc
    19861 ggattgaggc aagcgatgaa gatgaataag aaatcaagct acgtaatcaa tcgtttactt
    19921 ttagtcatca tagtactgat tttaggtact ctggctctag gaatcggtct aatggtaggt
    19981 tatggaatct tgggcaaggg tcaagatcca tgggctatcc tgtctccagc aaaatggcag
    20041 gaattgattc ataaatttac aggaaattag gctggagaac cagccttttt ctaaagataa
    20101 ggagaaatat gaacaaaaaa acaagacaga cactaatcgg actgctagtg ttattgcttt
    20161 tgtctacagg gagctattat atcaagcaga tgcagtcggc acctaatagt cccaaaacca
    20221 atcttagtca gaaaaaacaa gcgtctgaag ctcctagtca agcattggca gagagtgtct
    20281 taacagacgc agtcaagagt caaataaagg ggagtctgga gtggaatggc tcaggtgctt
    20341 ttatcgtcaa tggtaataaa acaaatctag atgccaaggt ttcaagtaag ccctacgctg
    20401 acaataaaac aaagacagtg ggcaaggaaa ctgttccaac cgtagctaat gccctcttgt
    20461 ctaaggccac tcgtcagtac aagaatcgta aagaaactgg gaatggttca acttcttgga
    20521 ctcctccagg ttggcatcag gtcaagaatc taaagggctc ttatacccat gcagtcgata
    20581 gaggtcattt gttaggctat gccttaatcg gtggtttgga tggttttgat gcctcaacaa
    20641 gcaatcctaa aaacattgct gttcagacag cctgggcaaa tcaggcacaa gccgagtatt
    20701 cgactggtca aaactactat gaaagcaagg tgcgtaaagc cttggaccaa aacaagcgtg
    20761 tccgttaccg tgtaaccctt tactacgctt caaacgagga tttagttccc tcagcttcac
    20821 agattgaagc caagtcttcg gatggagaat tggaattcaa tgttctagtt cccaatgttc
    20881 aaaagggact tcaactggat taccgaactg gagaagtaac tgtaactcag taaaagataa
    20941 gcctaaactc ctatgtcact tatggatgta ggagttcttt ttactagttt aagcagggct
    21001 agaacaggta ctaagaaaaa atagtaactt ctaaaactaa cttccagttt cccacaacct
    21061 agcttttagt atgagaaaaa cgcgtgaaat cagtggaaat tcgtcttaga ctaacttcca
    21121 gttttgggag agagatggaa gttacgctaa gaaaaaaata atatgtattg gattaaattt
    21181 tgtgatataa taaaggataa gatactattt taggagaaga actatggaag acccaagtag
    21241 tcagaatttg ttgctacaat ttgttttatt gtttatcttg acggtattaa atgccttttt
    21301 ctcagccaca gaaatggcca tggtttctct taatcgtgcc cgagttgaac aaaaggcgga
    21361 agaaggagac agacgctaca tccgcctgct gaaggtacta gaaaatccta accacttttt
    21421 atcaaccatt caggtaggaa tcaccctgat tacaatctta tcaggggcga gtttaactga
    21481 tactctagga cgtgtgattg cctcttggct tgggaatggc gaaacagcac aagccgtggc
    21541 aacttttcta tccttggcat ttttgaccta tatttccatc gtttttgggg aattatatcc
    21601 taagagaatc gctcttaatc taaaggatgc cttggcaatt cgtacagcgc cggttatcat
    21661 tgggatcgga aaactagtca gtccttttgt ttggctttta gctgcttcga ccaatttctt
    21721 gagccatttg actcctatgt cattcgatga tgcggatgaa aagatgaccc gtgatgaaat
    21781 tgcctatatg ctgacaaata gtgaagaaac attggatgct gatgagattg agatgctaca
    21841 aggtgtcttt tcgctcgatg aactgatggc acgagaggtt atggttcctc gaacggatgc
    21901 ctttatggtg gatattcagg atgatagtca agccattatc caaagtattt taaaacaaaa
    21961 ttattctcgt atcccggttt atgatgggga taaggacaat gtaattggaa tcattcacac
    22021 caagagtctc cttaaggcag gctttgtgga cggttttgac aatattgttt ggaagagaat
    22081 tttacaagat ccactttttg tacctgaaac tatttttgtg gatgacttgc taaaagaact
    22141 gcgaaatacc caaagacaaa tggcaatctt gctggatgaa tacggtggta tggctggttt
    22201 ggtgactttg gaagacctct tagaggaaat tgttggtgaa attgatgatg aaacggacaa
    22261 agcagcaata gatgttcatc agattggcga ggatacctac attgttcaag gaactatgac
    22321 cctcaatgat ttcaataatt actttaatgt tgaactggaa agtgatgacg ttgatactat
    22381 cgctggttat tatttgacag gagtgggaac cattccaacg actgagaaac tcagctatga
    22441 attgatcagc caaaacaaac agtttatctt aaccaatgat aaagtgaaga atggacgtgt
    22501 taccaaggta aaaatccaaa tcacagaagt cgaaatagaa gaagaaacag aataaactag
    22561 aggcttttgt caccaggcgt gacgaagctt ttttcaattg atttcaaaag ggaattatgc
    22621 catggaaaga actctgccac aaactcgctc caaaagtttt taaagttata cgtatttatt
    22681 cgagggaaaa taagaaaagc ccttcaaatt gggctttctg ttcatttgaa acctgataaa
    22741 tcaagttata gaaggcggta gacggatttg aaccgacgat caagcttttg cagagccgtg
    22801 ccttaccact tggctatacc gccttaacgt ttattattat accttgaaaa tgattttcag
    22861 tcaatagttt tttcagtttt ttgtaccata gcgtgattcg aatgtgatgc caagttttct
    22921 atgggaaaaa atttaccaaa aacctactga aaccccttga aaaatctagc taaatagggt
    22981 ataatatggg taatcatttg tcgtaggttt tgtctgaaat attgtccaga caaggctcac
    23041 agcagttaaa tcttctgaaa aagtcagatt tagctgctct ttttgtgctt tttttcaaga
    23101 ttttgagcat ttgtaacaga ggcttaaaga ttctgaaaat tcgtcaagag gacacggtga
    23161 taaggggttt acaaccatat ggcgattaga aaagcctgat tgacaaggct tggaacttat
    23221 ttacaaagga gaatcatctt ggcaggacat gacgttcaat acgggaaaca tcgtacccgt
    23281 cgtagttttt caagaatcaa agaagttctt gacttaccaa atttgattga aattcaaact
    23341 gactcattca aagctttcct agaccacggt cttaaggaag tgtttgaaga tgtattgcca
    23401 atttcaaact tcacagacac aatggagttg gaatttgttg gatatgaaat caaggaacca
    23461 aaatacacgc tagaagaagc tcgtatccac gatgctagct actcagcacc aatttttgta
    23521 accttccgtt tgatcaataa agaaacaggc gaaatcaaga cccaagaagt tttctttggt
    23581 gatttcccaa tcatgacaga aatgggtact ttcatcatca atggtggtga acgtattatc
    23641 gtttctcagt tggtccgctc accaggtgtt tactttaacg acaaagtaga caaaaatggt
    23701 aaggtgggct atggttcaac tgttatccct aaccgtggag cttggttgga acttgaaagc
    23761 gactcaaaag atatcaccta cactcgtatc gaccgtactc gtaagattcc atttacaacc
    23821 ttggttcgtg ctcttggttt ctcaggtgat gatgaaatct ttgatatctt tggtgacagc
    23881 gaattggttc gcaacactgt tgaaaaagat atccacaaga atccaatgga ctctcgtaca
    23941 gacgaagcct tgaaagaaat ttacgaacgc cttcgtccag gtgagcctaa gacggctgaa
    24001 agctcacgta gcttgcttgt agctcgcttc tttgacccac gtcgttatga cttggcagca
    24061 gttggtcgtt acaaaatcaa taaaaaactc aatgttaaaa cacgtttgct caaccaaacc
    24121 attgcagagc cattggtaga ccctgaaact ggagaaatct tggtagaagc tggtacgatt
    24181 atgactcgta gcgtgattga aagcattgaa agccatttgg atggcgactt gaacaagatt
    24241 gtctacatcc caaacgatgc agccgttgtg actgagcctg ttgttcttca aaaattcaag
    24301 gttattgctc caactgatcc agatcgcgtc gtaacgatca ttggtaatgc taacccagat
    24361 gacaaggttc gtacggtgac tcctgcagat atccttgctg agatgagcta cttcctcaac
    24421 ttggctgaag gacttggccg tgtagatgat atcgaccacc ttggaaatcg tcgtatccgt
    24481 gcggttggtg aattgcttgc caaccaagta cgtttgggac tttctcgtat ggaacgtaat
    24541 gtccgtgaac gtatgtctgt tcaggacaat gaagtcttga caccacaaca aattatcaat
    24601 attcgtcctg taacagctgc agttaaagaa ttctttggtt catcacagtt gtcacagttc
    24661 atggaccaac acaacccgct ttctgagttg tctcacaaac gccgtttgtc agccttagga
    24721 cctggtggtt tgactcgtga ccgtgccgga tatgaagtgc gtgacgtgca ctacactcac
    24781 tatggtcgta tgtgtccaat cgagacacct gaaggaccta acatcggttt gatcaataac
    24841 ttgtcatctt acggacactt gaacaaatat ggttttgttc aaacaccata ccgtaaggtt
    24901 gaccgtgaaa caggtgttgt cacgaacgaa attgtttggt tgacagctga tgaagaagat
    24961 gaatatactg tagctcaggc taactctcgt ctgaatgaag atggaacctt tgctgagaag
    25021 attgtcatgg gacgtcacca aggggtcaac caagagtatc cagctaatat tgttgactac
    25081 atggacgttt caccaaaaca ggtagttgcc gttgcgacag catgtattcc tttcttggaa
    25141 aacgatgact ccaaccgtgc cctcatggga gccaatatgc aacgtcaggc tgtgccattg
    25201 attaatcctc aggcacctta cgttggtact ggtatggaat accaagcagc ccacgattct
    25261 ggtgcggctg tgattgctca gtatgatggt aaagttactt acgcagatgc tgacaaggta
    25321 gaagttcgtc gtgaagatgg ttcattggat gtttaccaca tccaaaaatt ccgtcgttca
    25381 aactcaggta ctgcttacaa ccaacgcact ctcgtaaaag ttggtgatgt cgttgaaaaa
    25441 ggcgatttca tcgctgacgg accttctatg gaaaatggag aaatggcgct tggacaaaac
    25501 ccaatcgttg cctacatgac ttgggaaggc tacaacttcg aggatgccgt tatcatgagc
    25561 gaacgcttgg tgaaggacga tgtctacaca tctgttcacc ttgaagaata cgaatcagaa
    25621 acgcgcgata caaagcttgg gcctgaagaa atcactcgcg aaattccaaa cgttggtgaa
    25681 gatgccctca aagaccttga cgaaatgggg attatccgta ttggtgctga ggttaaagaa
    25741 ggtgatattc ttgtagggaa agtaacacct aagggtgaga aagatctttc agctgaagaa
    25801 cgtctcttgc acgctatctt tggagacaag tctcgtgaag tgcgtgatac ttctcttcgt
    25861 gtaccacacg gtgccgatgg tgtcgttcgt gatgttaaga tctttacacg tgtaaatgga
    25921 gatgagttgc aatcaggtgt taacatgttg gttcgtgttt acatcgctca aaaacgtaag
    25981 attaaggtcg gagataaaat ggccggacgt cacggaaaca aaggggttgt ctctcgtatc
    26041 gttcctgtag aagacatgcc ttaccttcca gacggaactc cagtcgacat catgttgaac
    26101 ccacttgggg tgccatcacg tatgaatatc ggtcaggtta tggagcttca ccttggtatg
    26161 gcagctcgta ctcttggtat tcacattgcg acaccagtct ttgatggagc aagttctgaa
    26221 gatctttggt caactgttaa agaagcaggt atggatagcg atgccaaaac gatcctttac
    26281 gatggacgta ctggtgaacc atttgataac cgtgtttctg ttggagtcat gtacatgatc
    26341 aaactccacc acatggttga cgataaattg cacgcgcgtt cagtcggacc ttactcaact
    26401 gttacccaac aaccactcgg aggtaaagct cagtttggtg gacaacgttt cggtgagatg
    26461 gaggtttggg ctcttgaagc ctacggtgcg tcaaatgtcc ttcaagaaat cttgacttac
    26521 aagtctgacg atatcaacgg acgtttgaaa gcctatgaag ctattacaaa aggcaaacca
    26581 attccaaaac caggtgttcc agaatccttc cgagttcttg ttaaagaatt gcaatctctt
    26641 ggtcttgaca tgcgtgtcct agacgaagat gaccaagaag tggaacttcg cgacttggat
    26701 gaaggaatgg acgaagatgt catccacgta gatgaccttg aaaaagcccg cgaaaaagca
    26761 gcccaagagg ctaaagcagc ctttgaagct gaagaagctg agaaagcaac aaaagcggaa
    26821 gcaacagaag aagctgctga acaagaataa gcagttcact tagaatagaa agggaagaaa
    26881 tagtggttga tgtaaatcgt tttaaaagta tgcaaatcac cctagcttct ccaagtaaag
    26941 tccgttcatg gtcttatgga gaagtcaaaa aacctgaaac aatcaattat cgtaccttga
    27001 aaccagaacg tgaaggactc tttgatgaag tgatctttgg tcctacaaaa gactgggaat
    27061 gtgcttgtgg taagtacaaa cgcattcgtt acagaggaat tgtttgtgac cgctgtgggg
    27121 ttgaagtaac gcgtacgaaa gttcgtcgtg agcgtatggg acatatcgaa ttgaaagctc
    27181 ctgtatctca catctggtac ttcaagggga ttccaagccg tatgggcttg acccttgata
    27241 tgagccctcg tgccctcgag gaagttatct actttgcggc ttatgtggtg attgatccta
    27301 aggatacacc acttgagcac aagtctatca tgacagagcg cgaataccga gagcgcttgc
    27361 gcgaatatgg ttatggttca tttgttgcca agatgggtgc ggaagccatc caagaccttt
    27421 tgaagcaagt agatcttgaa aaagaaattg ctgaactcaa agaagaattg aaaacagcta
    27481 ctggacaaaa acgtgtcaaa gccatccgtc gtttggatgt tttggatgcc ttttacaagt
    27541 ctggaaacaa acctgaatgg atgattctta acatccttcc ggttatccca ccagatcttc
    27601 gtccaatgtt gcagttggat ggtggccgtt ttgcctcatc tgacttgaat gacctttacc
    27661 gccgtgttat caatcgtaac aaccgtttgg ctcgtttgct tgagttaaat gcaccaggta
    27721 tcatcgttca aaatgagaaa cgtatgcttc aagaagcagt tgacgctttg attgacaatg
    27781 gtcgtcgtgg tcgtccaatc acaggaccag gtagccgtcc attgaaatca ttgagccaca
    27841 tgcttaaagg taaacaagga cgtttccgtc aaaacttgct cggtaaacgt gttgacttct
    27901 caggacgttc agttatcgcc gttggtccaa ctcttaagat gtaccaatgt ggtgtgccac
    27961 gtgaaatggc gattgaactc tttaaaccat ttgtcatgcg tgaaatcgtt gcccgtgata
    28021 tcgtgcaaaa cgtcaaagca gctaaacgct tggtggaacg cggagatgag cgtatctggg
    28081 atatccttga agaagtgatt aaagaacacc cagtgctttt gaaccgcgca ccgacccttc
    28141 accgtttggg tatccaagcc ttcgagccag tcttgattga tggtaaggct cttcgcttgc
    28201 acccacttgt ctgtgaagcc tacaatgctg actttgacgg ggaccaaatg gccatccacg
    28261 taccactttc agaagaagca caagcagaag ctcgtatcct catgctagct gctgagcaca
    28321 tcttgaaccc gaaagatggg aaaccggtag ttactccatc tcaggacatg gttttgggta
    28381 actactactt gaccatggaa gaagctggtc gcgaaggtga aggaatggtc ttcaaagacc
    28441 gtgacgaagc ggttatggct taccgcaatg gttatgttca cctccactca cgtgttggta
    28501 tcgcaacaga cagcctcaac aagccttgga cagaagagca aagacataag gtcttgctta
    28561 caacagttgg taaaattctc ttcaacgata tcatgccaga ggggctacca tacttgcaag
    28621 aaccaaacaa tgccaacttg acagaaggtg ttccagctaa atacttcttg ccacttggtg
    28681 gagatatcaa ggaagctatc agcaatcttg agctcaaccc tccattcaag aagaaaaacc
    28741 tcggaaatat catcgctgaa atcttcaaac gtttccgtac gacagaaact tctgccctac
    28801 ttgaccgcat gaagaacctc ggttaccacc actcaactct tgcaggattg acagtgggta
    28861 ttgccgatat cccagtcgtt gatgacaagg ctgaaatcat tgaagaatca cacaaacgtg
    28921 tcgaacaaat caccaaacaa ttccgtcgtg gtatgatcac agacgacgag cgttacaatg
    28981 ctgttacagc tgaatggcgt gctgcccgtg aaaaacttga gaaacgcttg attgctaatc
    29041 aagatcctaa gaacccaatc gttatgatga tggactctgg agcccgtggt aacatctcaa
    29101 acttctcaca gcttgccggt atgcgtggtc tgatggctgc tccgaatgga cgtatcatgg
    29161 aattgccaat cctttcaaac ttccgcgaag gtttgtcggt attggaaatg ttcttctcaa
    29221 ctcacggtgc tcgtaaaggt atgaccgata cggcccttaa gacagccgac tcaggttact
    29281 tgactcgtcg tttggtcgac gttgcccaag acgttatcat ccgtgaggac gactgtggaa
    29341 ctgaccgtgg tctcttgatc cgttctatcg cagaaggaaa agagatgatc gagtctctcg
    29401 aagagcgtct caacggtcgt tacactaaga aaactgttaa acatccagaa actggtgcag
    29461 tgattattgg tccaaatgaa ttgattacag aagacaaggc gcgtgaaatt gtcaatgctg
    29521 gtgtggaaga agtgactatc cgttctgtat ttacatgtaa cactcgtcac ggtgtctgcc
    29581 gtcactgtta cggtatcaac ttggcgactg gtgatgcggt tgaagttggt gaagcagttg
    29641 gtacaatcgc tgcccaatct atcggggaac ctggtacaca gcttacaatg cgtaccttcc
    29701 acacaggtgg ggttgcctca aataccgata tcactcaggg tcttcctcgt gtccaagaaa
    29761 tctttgaagc ccgcaatcct aaaggggaag cggttatcac agaggttaaa ggacaagtta
    29821 ctgctatcga agaggatgca tcaactcgta ccaagaaagt ctttgttaag ggtgaaactg
    29881 gcgaaggtga atatgtcgtt ccatttacag ctcgtatgcg tgtcgaagtt gggggccaag
    29941 tagcgcgtgg tgctgctctg acagaaggtt ctatccaacc aaaacgtctc cttgcagttc
    30001 gtgatgtctt gtcagttgaa acgtaccttc tcggtgaagt acaaaaagtt taccgtagcc
    30061 aaggggtaga aatcggtgac aaacacatcg aggtaatggt tcgtcaaatg atccgtaaag
    30121 tccgtgtcat ggatccaggt gatacagatc ttctcatggg taccctcatg gatatcaatg
    30181 actttacaga tgccaacaaa gatgtcctta tcgcaggtgg agttccagcg acaggtcgcc
    30241 cagtccttat gggaattacc aaagcctcac ttgaaacaaa cagtttcttg tcagcggctt
    30301 ccttccagga aacaactcgt gtccttactg acgcagctat ccgtggtaag aaagaccatc
    30361 tccttggact taaagaaaat gttatcatcg gtaagatcat cccagctggt actggtatgg
    30421 cccgttaccg taaccttgaa ccacatgctg tcaacgaaga agaatacctt aatcctccag
    30481 tagaggaaga aggaaatgaa gaaacaacag aagtagttgt ggatactgcc gttgaaactg
    30541 tggaagaaac agtagaataa gagagaatga gagaaccctc gggttctctc attctctctt
    30601 ctgaaaactt tctccatttt tccacgaaat gtggtaaaat aaaagaaaga agctgacaaa
    30661 ggagacgggt atggaacaaa cattctttat catcaaacca gatggtgtaa aaagagggct
    30721 agtgggtgaa gtgttaaagc gcatcgaaca acgtggattt acaatcgaaa aattggagtt
    30781 tcgttcacag gtttcagaag agttgattga ccagcactat caggacttgg ttggtcagag
    30841 tttttaccca ccgattcgtg aattcatgac ttcaggtcca gttcttgtgg gtgtcatttc
    30901 tggtcccaaa gtaatcgaaa cttggcggac catgatgggt gcaactcgtc cagaagaagc
    30961 tttaccaggc actattcgag gtgattttgc aaaagctgct ggagaaaatg agattatcca
    31021 aaatgttgta catggttcag attcagaaga gtcagctaag cgagaaattg ctctttggtt
    31081 ttaagagtgg attggctcaa tcaattggtt aaaagctcat ttgaatagaa agtatagtca
    31141 attagtttaa gacatgacgc atgatatcaa actttttagt ttttgatatg gtgcgttttt
    31201 tgattaagtg actattagtt tgtctcgctc cgttaggtgc gagacaaaaa aaacacccgc
    31261 tatgcgggtg cgcgtcgaag gttataccaa aaaaactcca aacgcgatac aataaaggtg
    31321 ttcaatccaa ttgtaaagcg aaaggagaaa aatatggcac aaaaggttct tttatcctgt
    31381 tcttgtttca attgactata tttctatact ttttcttcct ttctctgtga aaatcgcaag
    31441 tttttcccac cttttctcaa tactgaaaca acatctttcc agaacttcaa taatttcttt
    31501 ttgtgttaaa tcattattta actaaatctt tggaggcaat acgttttcaa agctactttt
    31561 ttaccatagg ttttgagcct tcaacacaga ttactaagcc aaaaatgctc taaatttttg
    31621 aaattctaac aaattccact caactttatc atcatctttg actttcacaa tatcttcctc
    31681 aaaataacat ggaataaaga tagttttctt ttaaaacggt tgtaggagga aaatctctcc
    31741 tacactatta ttgattctga gagaaaattg caaaatatca tctaccaaac tttcaattat
    31801 taagattttt tgcagtttat ctgaattaga atcgcaaggc ttctcaataa tataattgtc
    31861 gaattttttc attctaactt cctctccatt atttcttcct ttatcaagaa aaagttacct
    31921 caatattaat aaagtacaat tctaaattaa ctccaattct tgattcatga taatatttca
    31981 gtaactcatc gtgcaattaa aaacaaggat ttttatttca gatgatccat cgtgaacact
    32041 tcatcagcca tctcccaaat tgccggatta tgtgttgcga taatgattag cctattatca
    32101 tctcgaagag atagcaaaat ctccataatc aactgagagg ttgctgggtc tattgaagct
    32161 gttggctcat ctgccagaat aaagggtgga ttctttaaga taatttttgc caaggcaacc
    32221 cgttgcgatt ctccgcccga taactcaaag atgcgcttat ctaggtcaag ataagccagg
    32281 ccgacctgtt ctaaagcctg cttctgcctc aaccgctgtt ccgaccgact caacttttga
    32341 ccaatgagac ctagcttaag gttttcttca atactttggt tttcaattaa gccaaagttc
    32401 tggaagaggt agcccaattc gtgacggaaa aaatcactcg atttataatt ggccaagtct
    32461 ttacctcggt aaaaaatcgt cccatcataa ggttctaatt tcccaatcat gttcatcaag
    32521 gttgtttttc cgctaccact tgaaccaatt aaggcataga cttttccagc ctcaaatgtc
    32581 attgaaagat tcgaaaataa ctctcgttct ccaaaagatt tactcacctg tttaagttca
    32641 atcatattaa cctcccttca aaacaagaat ggacatcttg ttttctttct gcatttggac
    32701 atgtaactgt aaaagagata gaccagtaaa gagtaacaag actaagaaag caactcctat
    32761 ctctacctga agaaatacac tcgcaacaaa tcccagtaaa aacacaccca gttgagcaaa
    32821 gagataagtg cgatggattt ctaagaacct gagacctgca atgcgtttga taaaaatggc
    32881 acgtctaaat tcttcaaagt agagcctatt catagtgtta aacaacaaga ttgaagttgc
    32941 aatcccaagc acagctcctg ctagcattac ccaacgttcc ctctggatat tatccaataa
    33001 tgtgatgtag ttgtggtaac ctgtttgcat ttctgagacc caattttcaa tgccttgtct
    33061 ctggataagc tcctgggcat cagacaattg attaaagaga acgtagttct gtactgcgtc
    33121 tatccaaaac aaaatggact gtggaccagt tgattggggt gttataacaa tgatgattgg
    33181 atctttcaaa aactgctggt aagaaatagg ggtcgtatta tacacaaaac gatcctgacc
    33241 tgattctaaa taacctaccg tagcagtcat ttgctgtcgt tcatcttgac tagacatgcg
    33301 actggttaag tcgtcttcaa aaacagattt ataatgttct tcctctgaac ggaggtgttc
    33361 aggcagcaat aagacaaact cccctacatt aaggtgattc atcttttgct caatagttgt
    33421 atctacagga atgttttgac gctccaagta ttgcggtgtg acgataagga cattgccatt
    33481 cgggttgtaa tcgtgccatt ctgtagaggt tatcaagttt ttggaggaag ccatgccatt
    33541 ttgcaaagta cggtcaatta actggtgtct agataagaaa gccttttgtt ctgaaacagc
    33601 cagatccatc aattgatacc aagttctgag tttcctttga gcttgttcat caaaaccagg
    33661 actcgttcct tcacggctga ttgatagggt aatcagttgc ctttcctgac tccaagcctc
    33721 ttgtccaatc cgatgctgtt gccaggcttg ggaatacttt aagctactcc ctattcccag
    33781 cgttacaata ataatcgcta atagttgacc gattaaaatc aaactaatga ttcctctgac
    33841 aggcacttgc ccttttataa tctgcatcag gtgtatcttt tttatcccaa ctgcgaagag
    33901 ttgagcgaaa aacagggaga tcgacaacaa gatgagatta taactgagca agcctgctcc
    33961 tatggtcatt agggattgcg ttggaagcga cagaattttt tgcataagaa tggcgagcac
    34021 gccagctaaa ctgaaaccaa cagctatccc tttcaaatcc tcaccaactg gtctaaggaa
    34081 aatggaccac cgtttctctc ctgaaatgag gcgaatgccc gacgaccgta attggctaat
    34141 ttgactaatt aaagtcagtg ctacaaaggt taagatgaaa atcaatagac tgattaactg
    34201 aaatccgtta ctaaagatag ccattaacgt agatagttta gatggaattg tcaggttcat
    34261 gttagtcaag ccaagttgac ttagctcctc ccttagcaag tgaatatcta ggtgtccatc
    34321 gaatacaaaa tagtttgttt caacactact actttgagca tcttctagtt ttttttcctg
    34381 aagcccatca ggcaactttc cgtccccaaa ggtcgtataa gaaaaaactg tagtaccttc
    34441 tgagttagaa tcttggtgtt gaatcgcaat aaagctatct gtttcttctg ctagtttttc
    34501 caagcgtgta gcaacatgct gaaaagttgt ttcaggggaa gaatcacgta caacaacgtt
    34561 ggggtaataa tgggagacat atgtatcagt ccaaatggta aatacccaaa caaagaacaa
    34621 actagcaagc aggttggata tgagtataaa taattttttc atagtgcatt ccttattcta
    34681 aaacagaggg cagaaatacc cctgccctct ataaactaac aagcttacta aactattgat
    34741 aaaagaatct tcttcaatta ttctttacca tcccgcatca aatctaaatt ttaacatatt
    34801 attactcctc tgaatgaaat attatgaaaa tgagcgaaat gcaactacat attttaatta
    34861 gaaatctcag tctgaattat agtatcctat tttttcctcc tttctaactg cacgttcctt
    34921 tctacaggca gagacctaat tttataaacc aatcttaaat actttagatt ttgtaactca
    34981 taattttcaa cagttggtta gctatgaaag cattttatca aacatctcca ttcttatatc
    35041 tatctggtat tgttacaagc tctcatcaga atacctttct ctaaatagtt gaattttaaa
    35101 gtaatacgaa atcaatcagc cttcccaaca tcaagaccat tggacttctt ttgtgattgc
    35161 actcgaatct ccaattgtct attcttctga ataaactgtt ggtaaggaat tgtgttacta
    35221 aacataaaaa cacctagtaa tactaatata gctaattttt gaattccacg atttttcttc
    35281 attcgtgaaa cccttttctt tttccttg
//
LOCUS       DAVMEI010000006        34359 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVMEI010000006 DAVMEI010000000
VERSION     DAVMEI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.006"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            520..972
                     /locus_tag="VYQ24_000245"
     CDS             520..972
                     /locus_tag="VYQ24_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1967434.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            991..2328
                     /locus_tag="VYQ24_000246"
     CDS             991..2328
                     /locus_tag="VYQ24_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1967435.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2407..3291
                     /locus_tag="VYQ24_000247"
     CDS             2407..3291
                     /locus_tag="VYQ24_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1967436.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3291..4124
                     /locus_tag="VYQ24_000248"
     CDS             3291..4124
                     /locus_tag="VYQ24_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1967437.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4142..6235
                     /gene="nanB"
                     /locus_tag="VYQ24_000249"
     CDS             4142..6235
                     /gene="nanB"
                     /locus_tag="VYQ24_000249"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1967438.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPALIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGDSQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRVESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWRYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6247..7350
                     /locus_tag="VYQ24_000250"
     CDS             6247..7350
                     /locus_tag="VYQ24_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1967439.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVRLSEIVK"
     gene            7524..8222
                     /locus_tag="VYQ24_000251"
     CDS             7524..8222
                     /locus_tag="VYQ24_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1967440.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8241..9758
                     /locus_tag="VYQ24_000252"
     CDS             8241..9758
                     /locus_tag="VYQ24_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1967441.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9883..11211
                     /locus_tag="VYQ24_000253"
     CDS             9883..11211
                     /locus_tag="VYQ24_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967442.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11463..12329
                     /locus_tag="VYQ24_000254"
     CDS             11463..12329
                     /locus_tag="VYQ24_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1967443.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12345..13184
                     /locus_tag="VYQ24_000255"
     CDS             12345..13184
                     /locus_tag="VYQ24_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1967444.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13457..13909
                     /locus_tag="VYQ24_000256"
     CDS             13457..13909
                     /locus_tag="VYQ24_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1967445.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            14006..14350
                     /locus_tag="VYQ24_000257"
     CDS             14006..14350
                     /locus_tag="VYQ24_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967446.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14634..15278
                     /locus_tag="VYQ24_000258"
     CDS             14634..15278
                     /locus_tag="VYQ24_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1967447.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15447..16364
                     /locus_tag="VYQ24_000259"
     CDS             15447..16364
                     /locus_tag="VYQ24_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1967448.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLDTARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16382..17266
                     /locus_tag="VYQ24_000260"
     CDS             16382..17266
                     /locus_tag="VYQ24_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1967449.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17282..18133)
                     /locus_tag="VYQ24_000261"
     CDS             complement(17282..18133)
                     /locus_tag="VYQ24_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1967450.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18333..20390
                     /gene="pbp2b"
                     /locus_tag="VYQ24_000262"
     CDS             18333..20390
                     /gene="pbp2b"
                     /locus_tag="VYQ24_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1967451.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKVTTSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTAKDLKEIA
                     SQLLGYVSITSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKILETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGLKHDLKTGDLTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVGTSNLETAMGKLRATFGEYGLGAATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSVLHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20401..20997
                     /gene="recR"
                     /locus_tag="VYQ24_000263"
     CDS             20401..20997
                     /gene="recR"
                     /locus_tag="VYQ24_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1967452.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPVGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21173..22216
                     /locus_tag="VYQ24_000264"
     CDS             21173..22216
                     /locus_tag="VYQ24_000264"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1967453.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22300..23673
                     /locus_tag="VYQ24_000265"
     CDS             22300..23673
                     /locus_tag="VYQ24_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1967454.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGVELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQDLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLSANDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23696..24271
                     /locus_tag="VYQ24_000266"
     CDS             23696..24271
                     /locus_tag="VYQ24_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1967455.1"
                     /translation="MIALTDTGLTFTKEPFDRERYEDLRSLLSEMLNQVSDLDADEVA
                     ELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEVGYSPTENILKEI
                     EEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQENQEIADLQFFAID
                     QLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24362..25066
                     /locus_tag="VYQ24_000267"
     CDS             24362..25066
                     /locus_tag="VYQ24_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1967456.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25285..26658
                     /gene="ftsA"
                     /locus_tag="VYQ24_000268"
     CDS             25285..26658
                     /gene="ftsA"
                     /locus_tag="VYQ24_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1967457.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26675..27934
                     /gene="ftsZ"
                     /locus_tag="VYQ24_000269"
     CDS             26675..27934
                     /gene="ftsZ"
                     /locus_tag="VYQ24_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1967458.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27939..28610
                     /locus_tag="VYQ24_000270"
     CDS             27939..28610
                     /locus_tag="VYQ24_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1967459.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28620..29159
                     /locus_tag="VYQ24_000271"
     CDS             28620..29159
                     /locus_tag="VYQ24_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1967460.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29159..29422
                     /locus_tag="VYQ24_000272"
     CDS             29159..29422
                     /locus_tag="VYQ24_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1967461.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29419..30204
                     /locus_tag="VYQ24_000273"
     CDS             29419..30204
                     /locus_tag="VYQ24_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1967462.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30213..31001
                     /gene="divIVA"
                     /locus_tag="VYQ24_000274"
     CDS             30213..31001
                     /gene="divIVA"
                     /locus_tag="VYQ24_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1967463.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31349..34141
                     /locus_tag="VYQ24_000275"
     CDS             31349..34141
                     /locus_tag="VYQ24_000275"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1967464.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10539 a         6076 c         7506 g        10238 t
ORIGIN      
        1 tcttaaaagt ttttggtgaa acgagatgta gaaaggagat ttagccaaag agtctattag
       61 tgctagaata atagattaga attattttag aaaaacgaag tgagcagctt ataaattcaa
      121 gtccccaaat agattcatac tagtatcttt tgcaaaaaat aaagggcgac ttccttcatg
      181 aatatcaatt tcatctataa ggaaggtagc taattgaact aacttattta ttctgtttgt
      241 cgctagaaaa atcagacctc cttgtgaaga ttgaggagat actgaatgaa aatcaaagaa
      301 gaaactagca agctagcagc agattgccta aaacaccgct ttgaggttgt agataagact
      361 gacgtatata atccaaggtg aagcgactgt ggtttgaaga gattttcaaa gagtataggc
      421 tagagagtag tgtttttatg tccttctagt agaaaatgct agacagaaga atggggaact
      481 tggataggaa aaatagattg agaaaggagg ttagaagaga tgattattac aaaaattagc
      541 cgtttaggaa cttatgtggg agtcaatcca cattttgcaa cattaataga ttttctagaa
      601 aaaacaggac tagaaaattt aacagaaggt tcgattgcta tcgacggtaa tcgattgttt
      661 gggaattgct ttacttatct agcagatggt caagcagggg atttctttga aacacaccaa
      721 aaatatttgg atattcattt agttttggaa aacgaagaag ccatggctgt tacatcgccg
      781 gaaaatgtaa gcgttaccca agaatatgat gaagagaaag atattgaatt atacacaggg
      841 aaagtggaac agttggttca tttgagagct ggcgaatgcc tcatcacttt tccagaagat
      901 ttacatcaac ccaaggttcg tataaatgat gaacctgtga aaaaagttgt ctttaaagtt
      961 gcgatttctt aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt tttatgtcta
     1021 gctggaattg cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa agaagctaca
     1081 actagtactg aaccaccaac agaattatct ggtgagatta caatgtggca ctcctttact
     1141 caaggacccc gtttagaaag tattcaaaaa tcagcagatg ctttcatgca aaagcatcca
     1201 aaaacgaaaa tcaagattga aacattttct tggaatgact tctatactaa atggactaca
     1261 ggtttagcaa atggaaatgt gccagatatc agtacagctc ttcctaacca agtaatggaa
     1321 atggtcaact cagatgcttt ggtgccgcta aatgattcta tcaagcgtat tggacaagat
     1381 aaatttaacg aaactgcctt aaatgaagca aaaatcggag atgattacta ctctgttcct
     1441 ctttattcac atgcacaagt catgtgggtt agaacagatt tgttaaaaga acataatatt
     1501 gaggttccta aaacttggga tcaactctat gaagcttcta aaaaattgaa agaagctgga
     1561 gtttatggct tgtctgttcc gtttggaaca aatgacttaa tggcaacacg tttcttgaac
     1621 ttctacgtac gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc agacttgaca
     1681 agccaacttg ctcaagatgg tattaaatac tgggttaaat tgtataaaga aatctcacct
     1741 caagattctt tgaattttaa tgtccttcaa caagctacct tgttctatca aggaaaaaca
     1801 gcatttgact ttaactctgg cttccatatc ggaggaatta atgccaacag tcctcaattg
     1861 attgattcga ttgatgctta tcctattcca aaaatcaaag agtctgataa agaccaagga
     1921 attgaaacct caaacattcc aatggttgtt tggaaaaatt caaaacatcc agaagttgct
     1981 aaagcattct tagaagcact ttataatgaa gaagactacg ttaaattcct tgattcaact
     2041 ccagtaggta tgttgccaac tattaagggg attagcgatt ctgcagccta taaagaaaat
     2101 gaaactcgta agaagtttaa acatgctgaa gaagtaatta ctgaagctgt taaaaaaggt
     2161 actgctattg gttatgaaaa tggaccaagt gtacaagctg gtatgttgac taaccaacac
     2221 attattgaac aaatgttcca agatatcatt acaaatggaa cagatcctat gaaagcagca
     2281 aaagaagcag aaaaacaatt aaacgattta tttgaggctg ttcagtagat gtaaaagact
     2341 agaaaatagg tgggctagtg agctgaaaag ctctagccca attttgtaaa agaagggaga
     2401 aggagaatgg ttaaagaacg taatttaact cgctggatat ttgttttgcc agctatgatt
     2461 atcgtaggat tactctttgt ttatccgttt ttctcgagta ttttttatag ctttaccaat
     2521 aagcatttga ttatgcctaa ttataaattt gttggtttgg ctaactataa agctgtgcta
     2581 tcagatccca acttctttaa tgcgttcttt aattcaatta agtggaccat tttctcatta
     2641 gttggtcaag ttttagtagg gtttgtattg gctttagctc ttcacagagt acgccacttc
     2701 aagaaattat ataggacctt attgattgtt ccttgggcat ttcctaccat cgttattgcc
     2761 ttctcttggc agtggattct aaacggggtt tatggctact tacctaatct aatcgtaaaa
     2821 ttaggtttaa tggaacatac acctgcattt ttgacagata gtacatgggc attcctatgt
     2881 ttggtgttta tcaacatttg gtttggagca ccaatgatta tggttaatgt gctttcagct
     2941 ttgcaaacag taccagaaga acaatttgag gctgctaaga tagatggtgc ttcaagttgg
     3001 caggtgttca agtttatcgt ctttccacat attaaagtgg ttgtaggact tctagttgtt
     3061 ttgagaactg tatggatctt taataacttt gacattatct acctcattac tggaggtgga
     3121 ccagccaatg ctacaacgac gcttccaatt tttgcttaca acctgggttg gggaactaaa
     3181 ttgttgggtc gtgcttcagc agttacagta ttgctcttta tcttcttggt ggcgatttgc
     3241 tttatctact ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata atgaagaaga
     3301 aatccagtat ttatttagat attctctcac atgtactttt agttggtgcg accatcgttg
     3361 caattttccc attggtatgg attatcatat cttctgtcaa agggaaaggg gaattaactc
     3421 agtatccaac acgattttgg cctgaacagt ttacattaga ttatttcact catgttatca
     3481 acgatttgca cttcattgat aacattcgaa acagtttaat cattgccttg gctacaaccc
     3541 tcattgcgat tattatttct gctatggcag cctatggtat tgttcgattc tttcctaaat
     3601 tgggagcaat catgtcgaga ctactcgtca ttacctacat tttcccacca attttgttag
     3661 caattcccta ttcaattgcc attgctaaag ttgggttaac aaatagttta tttggcttga
     3721 tgatggttta tctatctttt agtgttccat atgcagtttg gctcttagtt ggatttttcc
     3781 aaacagttcc aattggaatt gaagaagcgg ctagaattga tggtgcaaat aaatttgtta
     3841 cgttttataa agttgtgcta ccgattgtag caccaggtat tgtagcaaca gctatttata
     3901 catttatcaa tgcttggaat gaattcctgt atgccttgat tttgattaac aatacaggaa
     3961 agatgacagt agcagtagcc cttcgttcac ttaatggttc agaaatacta gactggggag
     4021 atatgatggc agcgtctgtt attgtagttc ttccatcaat tattttcttc tctatcatcc
     4081 aaaataagat tgcaagtgga ttatcagaag gatctgtgaa gtagacgaaa gaaggaaaaa
     4141 aatgaataaa agaggtcttt attcaaaact aggaatttct gttgtaggca ttagtctttt
     4201 aatgggagtc cccgctttga ttcatgcgaa tgaattaaac tatggtcaac tgtccatatc
     4261 tcctattttt caaggaggtt catatcaact gaacaataag agtatagata tcagcccttt
     4321 gttattagat aaattgtctg gagatagtca gacagtagta atgaaattta aagcagataa
     4381 accaaactct cttcaagctt tgtttggcct atctaatagt aaagcaggct ttaaaaataa
     4441 ttacttttca attttcatga gagattctgg tgagataggt gtagaaataa gagacgccca
     4501 aaagggaata aattatttat tttctagacc agcttcatta tggggaaagc ataaaggaca
     4561 ggcagttgaa aatacactag tatttgtatc tgattctaaa gataaaacat acacaatgta
     4621 tgttaatgga atagaagtgt tctctgaaac agttgataca tttttgccaa tttcaaatat
     4681 aaatggtata gataaggcaa cactaggagc tgttaatcgt gaaggtaagg aacattacct
     4741 cgcaaaagga agtattgatg aaatcagtct atttaacaaa gcaattagtg atcaggaagt
     4801 ttcaaatatt cccttgtcaa atccatttca gttaattttc caatcaggag attctactca
     4861 agctaactat tttagaatac cgacactata tacattaagt agtggaagag ttctatcaag
     4921 tattgatgca cgttatggtg ggactcatga ttctaaaagt aagattaata ttgccacttc
     4981 ttatagtgat gataatggga aaacgtggag tgagccaatt tttgctatga agtttaatga
     5041 ctatgaggag cagttagttt actggccacg agataataaa ttaaagaata gtcaaattag
     5101 tggaagtgct tcattcatag attcatccat tgttgaagat aaaaaatctg ggaaaacgat
     5161 attactagct gatgttatgc ctgcgggtat tggaaataat aatgcaaata aagccgactc
     5221 aggttttaaa gaaataaatg gtcattatta tttaaaacta aagaagaatg gagataacga
     5281 tttccgttat acagttagag aaaatggtgt cgtttatgat gaaacaacta ataaacctac
     5341 aaattatact ataaatgata agtatgaagt tttggaggga ggaaagtctt taacagtcga
     5401 acaatattcg gttgattttg atagtggctc tttaagagaa aggcataatg gaaaacaggt
     5461 tcctatgaat gttttctaca aagattcgtt atttaaagtg actcctacta attatatagc
     5521 aatgacaact agtcagaata gagtagagag ttgggaacaa tttaagttgt tgcctccgtt
     5581 cttaggagaa aaacataatg gaacttactt gtgtcctgga caaggtttag cattaaaatc
     5641 aagtaacaga ttgatttttg caacatatac tagtggagaa ctaacctatc tcatttctga
     5701 tgatagtggt caaacatgga agaaatcctc agcttcaatt ccgtttaaaa atgcaacagc
     5761 agaagcacaa atggttgaac tgagagatgg tgtgattaga acattcttta gaaccactac
     5821 aggtaagata gcttatatga ctagtagaga ttctggagaa acatggtcgg aagtttcgta
     5881 tattgatgga attcaacaaa cttcatatgg cacacaagta tctgcaatta aatactctca
     5941 attaattgat ggaaaagaag cagtcatttt gagtacacca aattctagaa gtggccgtaa
     6001 gggaggccaa ttagttgtcg gtttggtcaa taaagaagat gatagtattg attggagata
     6061 ccactatgat attgatttgc cttcgtatgg ttatgcctat tctgcgatta cagaattgcc
     6121 aaatcatcac ataggtgtac tgtttgaaaa atatgattcg tggtcgagaa atgaattgca
     6181 tttaagcaat gtagttcagt atatagattt ggaaattaat gatttaacaa aataaaggag
     6241 aaaaacatgg ttaaatacgg tgttgttgga gcagggtatt ttggagctga attggctcgc
     6301 tatatgcaaa agaatgatgg agcagagatt actcttctct atgatccaga taatgcagag
     6361 gcgattgcag aagaattggg agcaaaagta gcaagttcct tagatgagtt ggtttctagc
     6421 gatgaagtag attgtgttat cgtcgcaact ccaaataatc ttcataagga accggttatt
     6481 aaggctgcac agcatggtaa aaatgttttc tgtgaaaaac caattgcgct ttcttatcaa
     6541 gattgtcgcg agatggtaga tgcgtgtaaa gaaaacaatg taacctttat ggcaggacat
     6601 attatgaatt tctttaatgg tgttcatcat gcaaaagaac tcattaatca aggagttatc
     6661 ggagacgttc tatattgtca tacagctcgt aatggttggg aagaacaaca accgtcagta
     6721 tcatggaaaa aaattcgtga aaaatcaggt ggtcacttgt atcaccacat ccatgaattg
     6781 gattgcgttc aattccttat ggggggcatg cctgaaactg taaccatgac aggtggaaat
     6841 gtggcccatg aaggtgaaca tttcggtgat gaagatgata tgatttttgt caatatggaa
     6901 ttttctaata agcgttttgc tttgttagaa tggggttcag cttatcgttg gggtgaacat
     6961 tatgtcttaa tccaaggaag caaaggtgcc atccgcttag acttattcaa ctgtaaagga
     7021 actcttaagc tagatgggca agaaagctat ttcttgattc acgaatcgca agaagaagat
     7081 gatgatcgga ctcgtatcta tcatagtaca gagatggatg gagcaattgc ttatggtaaa
     7141 ccaggtaaac gtactccatt atggctatca tctgtcattg ataaagaaat gcgctatctg
     7201 catgagatta tggaaggagc tccagtatca gaagaatttg caaaactttt gacaggtgaa
     7261 gctgccctag aagcaattgc tactgcagat gcttgtaccc agtctatgtt tgaagatcgc
     7321 aaagtaagat tgtcagaaat tgtaaaataa attttggtat tctcctatct ataggtcgac
     7381 ttgctcctct gaaagtactt ttagaggagc tgtttgactt tgctagtttt tgaaactgaa
     7441 atctattata ctacaaacta ttgaaagcgt tttaatttta aggtataata atctcataga
     7501 aataaagaaa aggaggaaag aggatgccac agattagcaa agaagccttg attgagcaaa
     7561 tcaaagatgg aatcatcgtt tcttgtcagg ctcttcctca tgaaccgctt tatacagaag
     7621 cgggaggggt gattcccttg ctggtcaaag cggctgagca aggtggagca gtcggtatcc
     7681 gagcaaacag tgttcgcgat atcaaggaaa ttaaggaagt cactaaactt ccaatcattg
     7741 ggattatcaa acgtgattat ccacctcagg aacccttcat cacggctact atgaaagaag
     7801 ttgatgaatt ggcagaactg gacatcgagg tgattgctct ggattgtacc aagcgtgaac
     7861 gctacgatgg tttggaaatt caagagttca ttcgtcaggt taaggagaaa tatcctaatc
     7921 agcttttgat ggctgatact agtatcttcg aagaagggct agcagctgta gaagcaggaa
     7981 ttgactttgt cggaacaacc ttatcaggct acacatccta cagtccaaaa gtagacggtc
     8041 cagattttga attgattaag aaactctgtg atgccggtgt agatgtcatt gcagaaggaa
     8101 aaattcatac accagaacaa gccaaacaaa tccttgaata tggagtgcga ggcatcgttg
     8161 ttggtggcgc cattactaga ccaaaagaga ttacagaacg cttcgttgct agtcttaaat
     8221 aagatgtgag agggagtttt atgtttaaag ttttacaaaa agttggaaaa gcttttatgt
     8281 tacctatagc tatacttcct gcagcaggtc tacttttggg gattggtggt gcactttcaa
     8341 acccaaccac gatagcaact tatccaatac tagacaatag tatttttcaa tcaatattcc
     8401 aagtaatgag ctctgcagga gaggttgtat tcagtaattt gtcactactt ctctgtgtgg
     8461 gattatgtat tggcttagcg aaacgagata aaggaaccgc tgcgttagca ggagtaactg
     8521 gttacttagt tatgactgca acgatcaaag ctttggtaaa actttttatg gcagaaggat
     8581 ctgcaattga tactggagtt attggagcat tagttgtcgg aatagttgcc gtatatttgc
     8641 acaaccgata taacaatatt caattacctt ccgctttagg attctttgga ggttcacgct
     8701 tcgttcctat tgttacatcg ttctcttcta tcttgattgg ctttgtcttc tttgttattt
     8761 ggccaccttt ccaacaactt cttgtttcta caggtggata tatttctcag gcgggtccaa
     8821 ttggaacttt tctatatgga tttttaatga gactttctgg agcagtaggc ttacatcata
     8881 taatttaccc tatgttttgg tatactgaac ttggtggtgt tgaaactatt gcaggacaaa
     8941 cagtggttgg agctcaaaaa atattttttg ctcaattagc cgatccggcc cattctggat
     9001 tatttacaga aggaacaagg ttttttgcag gtcgtttctc aacaatgatg ttcggtttac
     9061 cggctgcctg tttagcgatg taccatagtg ttcctaaaaa tcgtcgtaaa aaatacgcgg
     9121 gtttgttttt tggagttgct ttaacatctt ttattaccgg tattacagaa ccaattgaat
     9181 ttatgtttct attcgtcagt ccggttctat atgttgttca cgcattcctt gatggtgtta
     9241 gcttctttat tgcagacgtc ttaaatattt caataggaaa cacattttca ggaggtgtaa
     9301 tcgatttcac tttatttgga attttgcagg ggaacgctaa gacgaattgg gttcttcaga
     9361 ttccatttgg acttatttgg agtgttttgt attatattat ttttagatgg ttcattactc
     9421 aattcaacgt tctaacgcca gggcgaggag aagaagtaga ttctaaagaa atttctgaat
     9481 ccgcagattc aacttcaaat actgcagatt atttaaaaca ggatagccta caaattatca
     9541 gagccttggg tggatcaaat aatatagaag atgtagatgc ttgtgtgaca cgtttacgtg
     9601 tagctgtaaa agaagttaat caagttgata aagcactttt aaaacaaatt ggtgcagttg
     9661 atgtcttaga agtgaagggt ggcattcaag caatctatgg agcaaaagca atcttatata
     9721 aaaatagtat taatgaaatt ttaggtgtag atgattaagt acttactgac ttaataaaaa
     9781 acagaggaga gtgatggatg agtaggatga aatgaaatcg catacaagaa ataaagaact
     9841 cattatccaa gttggatacg cttattacat aggagaatac aaatgaaatt tagaaaatta
     9901 gcttgtacag tacttgcggg tgctgcggtt cttggtcttg ctgcttgtgg caattctggc
     9961 ggaagtaaag atgctgccaa atcaggtggt gacggtgcca aaacagaaat cacttggtgg
    10021 gcattcccag tatttaccca agaaaaaact ggtgacggtg ttggaactta tgaaaaatca
    10081 atcatcgaag cgtttgaaaa agcaaaccca gatataaaag tgaaattgga aaccatcgac
    10141 ttcaagtcag gtcctgaaaa aatcacaaca gccatcgaag caggaacagc tccagacgta
    10201 ctctttgatg caccaggacg tatcatccaa tacggtaaaa acggtaaatt ggctgagttg
    10261 aatgacctct tcacagatga atttgttaaa gatgtcaaca atgaaaacat cgtacaagca
    10321 agtaaagctg gagacaaggc ttatatgtat ccgattagtt ctgccccatt ctacatggca
    10381 atgaacaaga aaatgttaga agatgctgga gtagcaaacc ttgtaaaaga aggttggaca
    10441 actgatgatt ttgaaaaagt attgaaagca cttaaagaca agggttacac accaggttca
    10501 ttgttcagtt ctggtcaagg gggagaccaa ggaacacgtg cctttatctc taacctttat
    10561 agcggttctg taacagatga aaaagttagc aaatatacaa ctgatgatcc taaattcgtc
    10621 aaaggtcttg aaaaagcaac tagctggatt aaagacaatt tgatcaataa tggttcacaa
    10681 tttgacggtg gggcagatat ccaaaacttt gccaacggtc aaacatctta cacaatcctt
    10741 tgggcaccag ctcaaaatgg tatccaagct aaacttttag aagcaagtaa ggtagaagtg
    10801 gtagaagtac cattcccatc agacgaaggt aagccagctc ttgagtacct tgtaaacggg
    10861 tttgcagtat tcaacaataa agacgacaag aaagtcgctg catctaagaa attcatccag
    10921 tttatcgcag atgacaagga gtggggacct aaagacgtag ttcgtacagg tgctttccca
    10981 gtccgtactt catttggaaa actttatgaa gacaaacgca tggaaacaat cagcggctgg
    11041 actcaatact actcaccata ctacaacact attgatggat ttgctgaaat gagaacactt
    11101 tggttcccaa tgttgcaatc tgtatcaaat ggtgacgaaa aaccagcaga tgctttgaaa
    11161 gccttcactg aaaaagcgaa cgaaacaatc aaaaaagcta tgaaacaata gtccttagtt
    11221 attctataaa aagtagtttt ttaaagaacc taagagtgta tacccccttt tccctctaca
    11281 cagatagtgt aagaaaaggg ggcttttgtt taaaatgtaa gaaactgtca cgaaattaaa
    11341 atgaagttct tacataagcg aatcataaaa aatttcattt tgattttaaa acagttcaag
    11401 aaagtcaaaa aattattcta tttgaaagag aggtgccgac tgtgaaagtc aataaaatcc
    11461 gtatgcggga aacagtgatt tcctacgctt tcctagcacc agtattattc ttctttgtca
    11521 tctttgtgtt ggctccgatg gtgatgggct tcattacaag tttctttaac tactcaatga
    11581 ctaaatttga gtttgtaggc ttggataact atatccgtat gtttaaagat cctgtcttta
    11641 caaaatctct gattaacaca gttattttgg ttattggatc tgtaccagtt gttgttctat
    11701 tctcactctt tgtagcatct cagacctatc atcaaaatgt cattgccaga tccttctacc
    11761 gtttcgtctt cttccttcct gttgtaacgg gtagtgttgc cgtgacagtt gtttggaaat
    11821 ggatttatga cccactatca gggattctaa actttgtcct taagtccagc cacatcatca
    11881 gccaaaacat ttcttggttg ggagataaaa actgggcatt gatggcgatt atgattattc
    11941 tcttgaccac ttcagttggt cagcccatca tcctttatat cgctgccatg gggaatattg
    12001 acaattcact ggttgaagcg gcgcgtgttg atggtgcaac tgagtttcaa gttttttgga
    12061 agattaaatg gccaagcctt cttccaacaa ctctttatat tgcaatcatc acaacaatta
    12121 actcattcca gtgtttcgcc ttgattcagc ttttgacatc tggtggtcca aactactcaa
    12181 caagtacctt gatgtactac ctttacgaaa aagccttcca attgacagaa tacggctatg
    12241 ccaacacaat tggtgtcttc ttggcagtca tgattgctat cgtaagcttt gttcaattta
    12301 aagtacttgg aaacgacgta gaatactaaa gaaaggagac agctatgcaa tctacagaaa
    12361 aaaaaccatt aacagccttt actgttattt caacaatcat tttgctcttg ttgactgtgc
    12421 tgttcatctt tccattctac tggattttga caggggcatt caaatcacaa cctgatacaa
    12481 ttgttattcc tcctcagtgg ttccctaaaa tgccaaccat ggaaaacttc caacaactca
    12541 tggtgcagaa ccctgccttg caatggatgt ggaactcagt atttatctca ttggtaacca
    12601 tgttcttagt ttgtgcaacc tcatctctag caggttatgt attggctaaa aaacgtttct
    12661 atggtcaacg cattctattt gctatcttta tcgctgctat ggcgcttcca aaacaagttg
    12721 tccttgtacc attggtacgt atcgtcaact tcatgggaat ccacgatact ctctgggcag
    12781 ttatcttgcc tttgattgga tggccattcg gtgtcttcct catgaaacag ttcagtgaaa
    12841 atatccctac agagttgctt gaatcagcta aaatcgacgg ttgtggtgag attcgtacct
    12901 tctggagtgt agccttcccg attgtgaaac cagggtttgc agcccttgca atctttacct
    12961 tcatcaatac ttggaatgac tacttcatgc aattggtaat gttgacttca cgtaacaatt
    13021 tgaccatctc acttggggtt gcgaccatgc aggctgaaat ggcaaccaac tatggtttga
    13081 ttatggcagg agctgccctt gctgctgttc caatcgtcac agtcttccta gtcttccaaa
    13141 aatccttcac acagggtatt actatgggag cggtcaaagg ataatactct gcgaaaatcg
    13201 aatgcaaact acgtcagctt caccttgcca tacttaagta ttgcctgcgg ttagcttcct
    13261 agtttgttct tcaattttca ttgagtatag gaaaatcaat ctatcaagat acagaagtat
    13321 attttataga tttagagaat atagaggtta taagtgtcta caaaatggag ggtatgcagt
    13381 tactttatga agttttgtca gacgcttatc aacttaagaa tggttttagt taactatcag
    13441 aaacgaagga aagagtatga tttttgacga tttgaaaaac atcacctttt acaaagggat
    13501 tcatcccaat ttagacaagg ctatcgacta cctctaccaa catcgtaaag attcattcga
    13561 attaggaaag tatgagattg atggagataa agtctttcta gttgttcagg aaaatgtcct
    13621 caatcaagtt gagaataatc aatttgaaca ccataagaac tatgcagatt tgcatttgct
    13681 ggtagaagga catgaatatt cgagctacgg ttcacgtatc aaagacgagg cagtagcatt
    13741 cgacgaagcg actgacattg gctttgttca ttgtcatgaa cactacccac tcttgttggg
    13801 ttatcataat tttgcgattt tcttcccagg tgagccacat cagccaactg gctatgcagg
    13861 tatggaagaa aaggttcgaa aatatctctt taaaattttg attgattaaa aataggatga
    13921 attgtttttt tgtaaagctt tgacaatact ctaccatgaa attgatcttt gtgaggtaga
    13981 gaaatgagaa taaaatattt aaaaattggt atcttctaag catgctgcaa gagctagttt
    14041 cttagatgga caggggatta cagttgatga gatgtcctgg ataattaatg gcattgtgaa
    14101 tgcattgatt ggtagataca taaaattagg tacttatgca gctaagtatg gtattagtat
    14161 agcacgctcg atcttaagta gggcagctgc aactgcagca gcaagagtag gattactgac
    14221 caagatttct ggatggattt tacgagtagc tgtgaatgta gctgatgtat atggtaattt
    14281 tgccaacaat attgctgcag cttgggatgc atatgataaa attcctaaca atggtcgtat
    14341 aaacttttaa aatgcgagaa tgaaagcact ttgtattttt ttattgaata tgttagcttg
    14401 gacagtgctt gcaatgataa ttcgtggagg gctagatgga tttgataggc atacttggag
    14461 tactatttta attgcttcgc tgttcggggt atatgattat aagcccatag ataaaaatag
    14521 aaaaaagtcc aaaagaaaaa agaaaaaata gatttgttca tggtagggac ttatgaaagc
    14581 tttactgaca aaaaagaaaa cagtttacaa agaaaaatga tggaggagca aacatggcac
    14641 aaaaaggagt aagccttatc aaggcagcat ttgatacaga taactttctc atgcgtttta
    14701 gtgagaaggt cttggacatc gtgacagcca atcttctttt tgtcgtctct tgtttgccca
    14761 tcgtgacgat tggagtggct aaaatcagcc tctacgagac catgttcgaa gttaagaaga
    14821 gcagacgggt gcctgttttt aaaatctatc taagatcttt caagcaaaat ctgaaactag
    14881 gtcttcagtt aggtctgctt gagttgggca ttgtttcatt aagcctccta gatctttatc
    14941 tcttttgggg tcaaacagct ctgccctttc aattgctgaa agctatttgt ctaggcattc
    15001 tgattttcct tacgattgtg atgttggcta gctatccaat cgcggcacgt tatgatttaa
    15061 cttggaaaga aattcttcaa aaaggattga tgttggctag ttttaacttt ccttggttct
    15121 tcctcatgtt agccattctt gtcctcattg tgatggttct ttatctgtct gccttcagtc
    15181 tactcttagg tggctcagtc ttcctgcttt ttgggtttgg actattggtc tttatccaga
    15241 ctggattgat ggagaaaatt ttcgcaaaat accaatagga gccttatttc tgaaactact
    15301 ttcaaaggct ccaaacgcta ttctataagc gagaaactaa aatctaagtg tctataagat
    15361 attgaaagcc ttttcggtaa agtgtatact aaagaagtaa agaaaaatct gctttagcag
    15421 aaaataaaaa taataggaga aaatctatgt cagatttgaa aaaatacgaa ggtgtcattc
    15481 cagccttcta cgcatgttat gatgagcaag gagaagtaag cccagaacgt acgcgtgcct
    15541 tggttcaata cttcattgat aaaggtgttc aaggtcttta tgtcaatggt tcttctggtg
    15601 aatgtatcta ccaaagtgtt gaagaccgca agttaatctt ggaagaagtt atggcagttg
    15661 ccaaaggtaa attgaccatt attgcccatg ttgcttgcaa taatactaaa gatagtatgg
    15721 aacttgctcg ccatgctgaa agcttgggag tagatgctat tgcaacgatt ccaccaattt
    15781 atttccgctt gccagaatac tcagttgcca aatactggaa cgatatcagt tctgcagctc
    15841 caaacacaga ctacgtgatt tacaacattc ctcaattggc aggggttgct ttgactccaa
    15901 gcctttacac agaaatgttg aaaaatcctc gtgttatcgg tgtgaagaac tcttctatgc
    15961 cagttcaaga tatccaaacc tttgtcagcc ttggtggaga agaccatatc gtctttaatg
    16021 gtcctgatga gcagttccta ggaggacgcc tcatgggggc tagggctggt atcggtggta
    16081 cttatggtgc tatgccagaa ctcttcttga aactcaatca gttgattgcg gataaggacc
    16141 tagacacagc gcgtgaattg cagtatgcta tcaacgcaat cattggtaaa ctcacttctg
    16201 ctcatggaaa tatgtacggt gtcatcaaag aagtcttgaa aatcaatgaa ggcttgaata
    16261 ttggatctgt tcgttcacca ttgacaccag tgactgaaga agatcgtcca gttgtagaag
    16321 cggctgctgc cttgattcgt gaaaccaagg agcgcttcct ctaatctaaa aggaggtatt
    16381 tatgacatat tacgttgcaa ttgatatcgg tggaaccaac atcaagtatg gtttggttga
    16441 tcaagagggg caacttcttg aatcgcatga aatgccaact gaggcgcata agggtggacc
    16501 tcatatctta caaaagacca aagatatcgt agctagttat ttagaaaaag gcccagtagc
    16561 aggtgttgcc atatcttctg ctgggatggt ggatccggat aagggtgaga ttttctatgc
    16621 tgggccgcaa atccctaact acgcaggcac ccagttcaaa aaggaaatcg aagaaagctt
    16681 tactattcct tgtgagattg aaaatgatgt caactgtgca ggtcttgctg aggcagtatc
    16741 tggttcaggc aagggagcaa gtgtgacact ttgcttgacc attggaacag gtatcggtgg
    16801 ttgcttgatt atggatagga aagtcttcca tggttttagc aattcagcct gtgaagtcgg
    16861 gtatatgcat atgcaggatg gagcttttca agacttggct tctacaacag ctttagtgaa
    16921 atatgtagct gaagcccatg gagaagatgt tgatcagtgg aatggccgta gaattttcaa
    16981 agaagccact gaaggaaaca aaatctgcat ggaaggtatt gaccgtatgg ttgactatct
    17041 aggaaaaggt ctggcaaata tttgctatgt tgccaatcca gaagtggtta ttcttggtgg
    17101 tggtatcatg gggcaagagg ctatcctcaa acctaagatc cgcacagcct tgaaagaggc
    17161 tttggtacca agtttagcag aaaaaacacg attagaattt gcccatcacc aaaatacagc
    17221 agggatgttg ggtgcatatt atcattttaa gacaaaacaa tcctagtttg gctcagccaa
    17281 actaggattt tcttacacgt ttttgtctac gatagccgtt gagtttttta ttttcccagt
    17341 agctattaaa gattttttcc ttgctttcgc gattgatttc caaaaagtag gcataaatca
    17401 aatcgataaa gaagagcata ggaagttgag cggatattcg ttggatatag gagggttggc
    17461 tgtgggtggc tacaagaaca gtctctgtat aggtctggct atctttattg ggaacacttg
    17521 taaagagtac agtctttgcc cccatctcct tggcatctaa tagactatct aaaatagaag
    17581 gagttgagcc tgaaagtgag aagcccagta ctagacaatt ttcatccatg atgctggttg
    17641 tccaggcaaa gccgtcttgg tctgtcaaag cttcgcagac cacacctagt cgcataaaac
    17701 gtaatttcat ttcacgggcg acgaggccag aactccctgt tccaaagaag tagatacgct
    17761 cagcatcttc gattagctgg gcaattcgtt ctagttggat ttcgtcaatc aagtcttgtg
    17821 tttgttccct catattgcta taacttctga ggactcgttt ggtcagtgga ctgtgcttgg
    17881 agacttggtt ggcttgattt tctgcctgat gttggtattg gaaaataaat tctcggtagc
    17941 cagtaaagcc acacttttta gcaaagcggg tcaaagcagc ttgagaaata tgtaattttt
    18001 gggtgacttg ttgagaagat aaatcatctg taatcgtttc agcttgcaaa aaatagcgag
    18061 cgatttcttg ttctaggtct gtcatttctt caaaatgtga atcaatgata gttgcgatat
    18121 ctggtttgtc catagggaaa gctcctttac atgagtcata ctggaagact agatcaggga
    18181 atagtcacac ttcattataa cacataatat aaggatagat aaataaaaac gcatctctgt
    18241 tttaaaaacg aaaaaatcga aaaagcttct tacttttcca taattttcta ctcaaattgt
    18301 ggtacaatta agagtaagat tttaagttag aaatgagact gatttgtatg agaaaattta
    18361 acagccattc gattccgatt cggcttaatt tattgttttc aatcgtcatt ttactcttta
    18421 tgaccattat tggtcgtttg ttgtatatgc aagttttgaa caaggatttt tacgaaaaaa
    18481 agctagcctc agctagtcag accaaggtca caaccagttc tgcccgtggg gaaatctatg
    18541 atgctagtgg aaaacctttg gtagaaaata cgttaaagca ggttgtttcc tttacgcgta
    18601 gtaataaaat gacggctaaa gatctgaaag aaatcgctag tcagttattg ggatatgtga
    18661 gcatcacttc gccgaattta acagaacgcc agctagcaga ctactattta gctgatcctg
    18721 aaatctataa aaaaacagtg gaggctctcc caagtgagaa gcgcttggat tcagatggca
    18781 atcgtttgtc cgaatcagaa ctgtataaca atgcggtcga tagtgtccaa acgagtcaac
    18841 taaactatac agaggatgaa aagaaagaaa tctatctttt tagtcagtta aatgctgttg
    18901 gaaactttgc gacaggaacc attgcgacag atcctctaaa tgattctcag gtggctgtta
    18961 ttgcctctat ttcaaaggag atgcctggca ttagtatttc tacttcttgg gatcgaaaga
    19021 ttttggaaac ttccctttct tctatagtag ggagtgtatc cagtgaaaaa gctggtctcc
    19081 cagcggaaga agcagaagcc tatcttaaaa aaggctattc tctaaatgat cgggttggaa
    19141 cctcctattt ggaaaagcaa tacgaggaaa ccttgcaagg taaacgctcg gtaaaagaaa
    19201 tccatctgga taaatatggc aatatggaaa gcgtggatac aattgaggaa ggtagtaagg
    19261 gaaacaatat caaactgacc attgatttgg ccttccaaga tagcgtggat gctttgctga
    19321 aaagttattt caattccgag ctaggaaatg gtggagccaa gtattctgag ggtgtgtatg
    19381 cagtcgccct taaccccaaa acaggtgctg ttttgtctat gtcaggactc aaacatgacc
    19441 tgaaaacggg agacttgacg cctgattcct tgggaacggt aaccaatgtc tttgtcccag
    19501 ggtcagtagt taaggccgct accatcagct caggttggga aaatggtgtt ttatcaggaa
    19561 accaaacctt aacagatcag cctattgttt tccaaggttc agctccaatt tattcttggt
    19621 ataaattggc atatggatct tttcctatta cagctgtgga agccttggag tattcatcta
    19681 atgcttacat ggttcaaacc gctcttggaa tcatgggcca gacctatcaa ccaaatatgt
    19741 ttgttggaac cagcaatttg gaaacagcta tgggaaaact tcgtgcgacc tttggcgaat
    19801 atggcttggg ggctgcgacc ggaattgacc taccagatga atctactgga tttgttccca
    19861 aagagtatag ctttgctaat tacatcacca atgcctttgg gcagtttgat aactatacgc
    19921 ccatgcagtt ggctcagtat gtagcaacta ttgcaaatga tggtgttcgt gtggctcctc
    19981 gtattgttga aggtatttat ggaaataatg ataagggagg actgggcgac ttgattcagc
    20041 aactgcaacc gacagagatg aataaggtca atatatccga ctccgatatg agtgtcttgc
    20101 accaaggttt ttatcaggtt gctcatggga ctagcggatt gacaactgga cgtgcctttt
    20161 caaatggtgc cttggtatcc attagcggaa aaacgggtac agccgaaagc tatgtggcag
    20221 atggtcagca agcaaccaat accaatgcgg tggcctatgc cccatctgat aatccccaaa
    20281 tcgctgttgc agtggtcttt cctcataata ccaatctaac aaatggtgta gggccttcca
    20341 ttgcgcgtga cattatcaat ctgtatcaaa aataccatcc gatgaactag aaaggaaatt
    20401 atgctttatc caacacctat tgccaagttg attgacagtt attctaagtt accaggtatc
    20461 gggattaaga cggctacgcg tctggccttt tatacgattg ggatgtctgc tgatgatgtc
    20521 aatgaatttg caaaaaatct cctttctgct aagagagaat tgacatattg ttctatttgt
    20581 ggacgtttga cagacgacga tccttgttct atctgtactg atccgactcg tgaccagaca
    20641 acaattttag ttcttgagga tagtagagat gtggcagcca tggaaaatat ccaagaatac
    20701 catggactct atcatgtcct gcatggcctc atttctccta tgaatggtat cagtccggac
    20761 gatatcaatc tcaagagcct tatgactcgt cttatggata gtgaggtttc agaagtgatt
    20821 gtggcgacta atgctacagc ggatggtgaa gcgacttcca tgtatctttc acgtttgctc
    20881 aagccggttg gtatcaaggt tacgcgtcta gcacgaggtc tcgctgtggg agcggacatt
    20941 gagtatgcgg acgaagtgac actcttacga gcgattgaaa atcggacaga gttgtaagtg
    21001 taggcaaatt tacgaactcc cttcatttat aaaaaatcaa agaggctgaa aatcgttcct
    21061 atcggcctct ttttgtatag tgtgatgagt aggatcgggt tcaagtttta aaaaaccaag
    21121 caaatatgat atactaaaga gcgagtattc tagtagaatt aggacaaata atatgaaaca
    21181 aacgattatt cttttatatg gtggacggag tgcggaacgc gaagtctctg tcctttcagc
    21241 tgagagtgtc atgcgtgcgg tcaattacga ccgtttcaca gtcaagactt tctttatcag
    21301 tcagtcaggt gactttatca aaacacagga atttagtcat gctccggggc aagaagaccg
    21361 tctcatgacc aatgaaacca ttgattggga taagaaagtt gcaccaagtg ctatctacga
    21421 agaaggtgca gtggtctttc cagtccttca cgggccaatg ggagaagatg gctctgttca
    21481 aggattcttg gaagttttga aaatgcctta cgttggttgc aacattttgt catcaagtct
    21541 tgccatggat aaaatcacga ctaagcgtgt tctggaatct gctggtattg cccaagttcc
    21601 ttatgtggct atcgttgaag gcgatgatgt gactgctaaa atcgctgaag tggaagaaaa
    21661 attggcttat ccagtcttca ctaagccgtc aaacatgggg tctagtgtcg gtatttctaa
    21721 gtctgaaaac caagaagaac tccgtcaagc cttaaaactt gccttccgat atgacagccg
    21781 tgtcttggtt gagcaaggag tgaatgcccg tgaaattgag gttggcctct tgggtaacta
    21841 cgatgtcaag agcacgctac ctggagaagt tgtcaaggac gttgcctttt atgactacga
    21901 tgccaagtat attgataaca agattactat ggatattcct gccaaaatca gtgatgatgt
    21961 ggtggctgtc atgcgtcaaa atgcagaaac agccttccgt gccattggtg gccttggtct
    22021 atctcgttgc gatttcttct atacagataa gggagagatt tttctcaacg agctcaatac
    22081 catgccaggt ttcacccagt ggtctatgta cccactactt tgggacaata tggggatcag
    22141 ctacccagaa ctaatcgagc gtttggttga ccttgccaag gaaagttttg acaagcgcga
    22201 agcgcatttg atataaaatg aaagagaggg tagaagccag aaccatcact gcaaggtgac
    22261 tagagttctc ggacttcagc cctttttaaa ggagtagaaa tgaaattaac aatccatgaa
    22321 attgcccaag ttgttggagc caaaaatgat atcagtatct ttgaggacac ccagttagaa
    22381 aaagctgagt ttgatagtcg tttgattgga actggagatt tatttgtgcc acttaaaggt
    22441 gcgcgtgacg gtcatgactt tatcgaaaga gcttttgaaa atggtgcggc agcaaccctg
    22501 tctgagaaag aggttgcaaa tcatccctac attctagtag atgatgtttt gactgccttt
    22561 caacaactag ctgcctacta tcttgaaaaa acagctgttg atgtctttgc ggttacgggt
    22621 tcaaatggca agacaacgac taaggatatg ttggcgcatt tactgtcaac gacctacaag
    22681 acctacaaaa cacaaggcaa ctacaataac gagattggcc ttccctacac agttctacac
    22741 atgcctgaag gaacagaaaa gttggttttg gagatggggc aggaccactt gggcgatatt
    22801 catctcttgt ctgaattggc tcgtccaaaa acagccatcg tgaccttggt tggagaagct
    22861 catttagcct ttttcaaaga tcgttctgag attgctaaag gaaaaatgca aattgcagac
    22921 ggaatggctt caggttcctt gcttttagcg ccggctgacc ctatcgtaga ggactatttg
    22981 ccaactgata aaaaggtggt tcgttttggg caaggggtag agctggaaat tactgacttg
    23041 gttgagcgca aagatagtct gaccttcaag gccaatttct tagagcaagt ccttgatttg
    23101 ccagtaactg gcaagtacaa tgcgacaaat gctatgattg catcctatgt tgccttgcaa
    23161 gaaggagttt cagaggagca aattcgtttg gccttccaag atcttgaatt gacgcgtaac
    23221 cgtaccgagt ggaagaaagc agccaatgga gcagatatcc tgtcagatgt ttacaatgcc
    23281 aatccaactg ctatgaaact gattttagag actttctctg ccattccagc caatgaaggt
    23341 ggcaagaaaa ttgcagtttt ggcggatatg aaggagcttg gtgaccagtc tgttcaactt
    23401 cataatcaga tgattttgag cctctcccca gatgtgcttg atatcgttat tttctatgga
    23461 gaagacattg ctgaattagc ccaattggcc agtcaaatgt tcccaattgg ccacgtttac
    23521 tacttcaaga aaacagaaga ccaagaccaa tttgaagact tagtcaagca ggtcaaggaa
    23581 agcctcagtg ccaatgatca aatcttgctc aagggctcta actctatgaa cctagccatg
    23641 ttggtagaaa gtttagaaaa tgaaaccaag tgattttgtt aagtatctgt aaagaatgat
    23701 tgcccttaca gatactggct taacctttac aaaagagcct tttgaccgtg agcgctacga
    23761 agacttgcga agtctgttat ctgaaatgtt gaatcaggta tcagaccttg atgcagacga
    23821 agtggcagaa ctcttgaaac cgacctccgc ttatgcgact cccttaatgg acgtccgtgc
    23881 ttggattgtt gaggatgaga agatttgtct ggttagggga caaggagagg atagttgggc
    23941 tttgccaggt ggctttggtg aagtcggcta ttctccaact gaaaatattc tcaaggaaat
    24001 tgaagaagaa accggtttta aagccaaagt tgaaagactg ctagctgttt ttgataccaa
    24061 tcgtttccaa ctacagagca aacaatatgc aaagtttgtt tttgaatgta agcttctcga
    24121 tggacaattc caagaaaatc aagaaatcgc tgaccttcaa ttttttgcaa ttgaccaact
    24181 gccgaattta tctgaaaaac gcattaccaa ggagcaaatc gagattcttt ggcaggttta
    24241 tcaaggtcag agggagcaat atcttgacta agaagatgat tatcgtattt ctaaattcat
    24301 ttttcttaag tagcatggta taataaaatg caggaaaatt ttgaatcatg aggaagacta
    24361 gatgaattta tgggatattt tctttacgac tcaggcaacc gagccgccca aatttgacct
    24421 tttttggtat gttagcctat ttacgctctt agccttaacc ttttatacag cccatcgcta
    24481 tcgtgaaaag aaggtttacc aacgattttt ccaaatcttg cagactgttc agttaatcct
    24541 tctttatggt tggtactggg tcaatcatat gccactgtca gaaagcctac ccttttacca
    24601 ttgccgtatg gctatgtttg tggtactctt gcttcctggt cagtccaaat ataaacaata
    24661 ctttgcatta ttgggaacat ttgggacatt agcagccttt gtttatccag tgccagatgc
    24721 ttaccctttt ccacatatca ccattctatc ctttatcttt ggtcatttag cactcttggg
    24781 gaactctcta gtttatctat tgagacagta taatgcgcga ttgctggatg tgaagggaat
    24841 ttttctcatg acctttgccc taaatgcctt gatttttgtg gtcaatttgg tgacaggtgg
    24901 cgattacgga tttttgacaa aaccgccatt ggttggggat cacggtctag tagctaatta
    24961 tttacttgtt tcaattgtgc tggtagctac tatcagtttg actaagaaaa tcttagaatt
    25021 ctttttagct caagaagcag aaaaaatgat tgcaaaggaa gcttaacaca gagctttctt
    25081 ttttgctctt agagagtttt tacaagcagc ttataaaata agaatttctg aatagacaaa
    25141 ctcaaaaaat ggctgggaaa tttaggaaaa aagcaagcac gattaaattt tttgtgttat
    25201 aatattttgt gaatagctat gcctatgttt agctatggaa taatacgaag tgcgaaactt
    25261 ggaagataga gaggaagcga tgtaatggct agagaaggct tttttacagg tctagatatt
    25321 ggaacaagct ctgtcaaggt gcttgtggcc gagcagagaa atggtgaatt aaatgtaatt
    25381 ggcgtgagta atgccaaaag taaaggtgta aaggatggaa ttattgttga tattgatgca
    25441 gcagcaactg ctatcaagtc agccatttcc caagcggaag aaaaggcagg catttcgatt
    25501 aaatcagtaa atgtcggctt gcctggtaat cttttgcagg tagaaccaac tcaggggatg
    25561 attccagtaa catctgatac taaggaaatt acggatcaag atgttgaaaa tgttgtcaaa
    25621 tcagctttga caaagagtat gacacctgac cgtgaagtca ttacctttat tcctgaagaa
    25681 tttattgtgg atggtttcca agggattcgt gacccacgtg gcatgatggg ggttcgcctt
    25741 gaaatgcgtg gtttgcttta tacaggacct cgtactatct tgcacaattt gcgtaagacg
    25801 gttgagcgtg caggtgttca ggttgaaaat gttatcattt caccactagc aatggttcag
    25861 tctgttttga acgaagggga acgtgaattt ggtgctacag tgattgatat gggggcaggt
    25921 caaacgactg tcgctacaat ccgtaatcaa gaactccagt tcacacatat tctccaagaa
    25981 ggtggagatt atgtaactaa agatatctcc aaggttttga aaacctctcg caaattagcg
    26041 gaaggcttga aactgaatta cggggaagcc tatccgcctc ttgcaagcaa agaaaccttc
    26101 caagtagagg ttattggaga agtagaagca gtcgaagtga cggaagccta cttgtcagaa
    26161 attatttctg cacgaatcaa gcacatcctt gaacaaatca agcaagaatt agatagaagg
    26221 cgtctattgg acctccctgg tggtattgtc ttaatcggtg ggaatgccat tttaccaggt
    26281 atggttgagc ttgctcagga agtctttggc gtccgtgtca agctttatgt tccaaatcaa
    26341 gttggtatcc gtaatccagc ctttgcgcat gtgattagtt tatcagaatt tgcgggtcaa
    26401 ttaacagaag ttaatctttt ggctcaggga gcgataaaag gtgagaatga cttaagtcat
    26461 cagccaatta gttttggtgg gatgctgcaa aaaacagctc agtttgtaca atcaacgcct
    26521 gttcaaccag ctcctgctcc agaagtagag ccggtggcgc ctacagaacc aatggcggat
    26581 ttccaacaag cttcacaaaa taaaccgaaa ttagcagatc gtttccgtgg attgatcgga
    26641 agcatgtttg acgaataaag aggaaaaata aattatgaca ttttcatttg atacagctgc
    26701 tgctcaaggg gcagtgatta aagtaattgg tgtcggtgga ggtggtggca atgccatcaa
    26761 ccgtatggtc gacgaaggtg ttacaggcgt agaatttatc gcagcaaaca cagatgtaca
    26821 agcattgagt agtacaaaag ctgagactgt tattcagttg ggacctaaat tgactcgtgg
    26881 tttgggtgca ggaggtcaac ctgaggttgg tcgtaaagcc gctgaagaaa gcgaagaaac
    26941 actgacggaa gctattagtg gtgccgatat ggtcttcatc actgctggta tgggaggagg
    27001 ctctggaact ggagctgctc ctgttattgc tcgtatcgcc aaagatttag gtgcgcttac
    27061 agttggtgtt gtaacacgtc cctttggttt tgaaggaagt aagcgtggac aatttgctgt
    27121 agaaggaatc aatcaacttc gtgagcatgt agacactcta ttgattatct caaacaacaa
    27181 tttgcttgaa attgttgata agaaaacacc gcttttggag gctcttagcg aagcggataa
    27241 cgttcttcgt caaggtgttc aagggattac cgatttgatt accaatccag gattgattaa
    27301 ccttgacttt gccgatgtga aaacggtaat ggcaaacaaa gggaatgctc ttatgggtat
    27361 tggtatcggt agtggagaag aacgtgtggt agaagcggca cgtaaggcaa tctattcacc
    27421 acttcttgaa acaactattg acggtgctga ggatgttatc gtcaacgtta ctggtggtct
    27481 tgacttaacc ttgattgagg cagaagaggc ttcacaaatt gtgaaccagg cagcaggtca
    27541 aggagtgaac atctggctcg gtacttcaat tgatgaaagt atgcgtgatg aaattcgtgt
    27601 aacagttgtc gcaacgggtg ttcgtcaaga ccgcgtagaa aaggttgtgg ctccacaagc
    27661 tagatctgct actaactacc gtgagacagt gaaaccagct cattcacatg gctttgatcg
    27721 tcattttgat atggcagaaa cagttgaatt gccaaaacaa aatccacgtc gtttggaacc
    27781 aactcaggca tctgcttttg gtgattggga tcttcgccgt gaatcgattg ttcgtacaac
    27841 agattcagtc gtttctccag tcgagcgctt tgaagcccca atttcacaag atgaagatga
    27901 attggataca cctccatttt tcaaaaatcg ttaagtaaat gaatgtaaaa gaaaatacag
    27961 aacttgtttt tcgagaagtt gcagaggcta gtctgagtgc tcatcgagag agtggttcgg
    28021 tctctgtcat tgcagttacc aagtatgtag atgtaccgac agcggaagcc ttgcttccgc
    28081 taggtgttca tcatatcggt gaaaatcgtg tagataagtt tctggaaaaa tatgaagctt
    28141 taaaagatcg agatgtgact tggcatttga ttggtacctt gcaaagacgt aaggtgaaag
    28201 atgtcattca atacgttgat tatttccatg cattggactc agtaaagcta gcaggggaaa
    28261 ttcaaaaaag aagtgaccga gtcatcaagt gtttccttca agtaaatatt tctaaagaag
    28321 aaagcaaaca cggtttttcg agagaggaac tgctggaaat cttgccagag ttagccagac
    28381 tagataagat tgaatatgtt ggtttaatga cgatggcacc ttttgaggct agcagtgagc
    28441 agttgaaaga gattttcaag gcggcccaag atttacaaag agaaattcaa gagaaacaaa
    28501 ttccaaatat gcctatgacc gagttaagta tgggaatgag tcgtgattat aaagaagcga
    28561 ttcaattcgg ttccactttt gttcgtatag gtacatcatt ttttaagtag gagagaacca
    28621 tgtctttaaa agatagattc gatagattta tagattattt tacggaggat gaggattcaa
    28681 gtctccctta tgaaaaaaga gatgagcctg tgtttactcc agtaaattct tcacaggaac
    28741 cggctctccc aatgaatcaa ccttcacagt cggctggtac aaaagagaac aatatcacca
    28801 gacttcatgc aagacaacag gaattggcaa atcagagtca gcgtgcaacg gataaggtca
    28861 ttatagatgt tcgttatcct agaaaatatg aggatgcaac agaaattgtt gatttattgg
    28921 caggaaacga aagtatcttg attgattttc agtatatgac agaggtgcag gctcgtcgtt
    28981 gtttggacta tttggatgga gcttgtcatg ttttagctgg aaatttgaaa aaggtagctt
    29041 ctaccatgta tttgttgaca ccagttaatg ttattgtaaa tgttgaagat atccgtttac
    29101 cagatgaaga tcaacagggt gagttcggtt ttgatatgaa gcgaaataga gtacgataat
    29161 gattttttta attcgtatga tttataatgc agtggatatt tactccctga ttttggtagc
    29221 cttcgctgtc atgtcttggt ttccaggtgc ctacgaatcc agtttaggtc gttggattgt
    29281 agcgttggtg aaaccagtac ttgctccctt gcaacgcctg cctttacaga tagcgggtct
    29341 tgatttatct gtttgggtcg cgattgtttt ggttcgattt ttaggagaaa acctagtgcg
    29401 ttttctggcg atgataggat gaataaaggg atttatcagc atttctccat agaagatcgt
    29461 ccatttcttg acaagggaat ggaatggata aagaaggtag aagatagcta tgctcctttt
    29521 ttaactcctt ttatcaatcc tcatcaggag aagctattaa agattttggc caaaacctat
    29581 ggtcttgctt gtagcagtag tggggaattc gtctcgagtg agtatgttcg agttttatta
    29641 tacccagatt atttccaacc agagttttca gattttgaaa tatctctcca agaaattgtg
    29701 tattccaata aatttgaaca tttaacgcat gctaagattt tagggacagt catcaatcaa
    29761 ttagggattg aacggaaact ttttggagat atcctagtag atgaagaacg ggcgcagatt
    29821 atgattaatc agcagtttct tcttctcttt caagatggac taaagaaaat tggtcgtata
    29881 cctgtttcgc tggaggaacg tcctttcacc gagaaaatag ataagctaga acagtatcga
    29941 gaactggatt tatctgtgtc tagttttcga ttagatgttc ttttatcaaa tgttttgaaa
    30001 ctatctagga atcaagcaaa ccagttgatt gaaaagaaac ttgtccaagt aaattatcat
    30061 gtggtagaca aatcagatta cactgttcaa gttggagact tgattagtgt gagaaaattt
    30121 ggtcgcttga gattacttca agataaggga caaacgaaaa aagagaagaa aaaaataacc
    30181 gtccagttat tattaagtaa gtgaggaata gaatgccaat tacatcatta gaaataaagg
    30241 acaagacttt tggaactcga ttcagaggtt ttgatccaga agaagtcgat gaatttttag
    30301 atattgtggt tcgtgattac gaagatcttg tgcgtgcgaa tcatgataaa aatttgcgta
    30361 ttaagagttt agaagagcgt ttgtcttact ttgatgaaat aaaagattca ttgagccagt
    30421 ctgtattgat tgctcaggat acagctgaga gagtgaaaca ggcggcgcat gaacgttcaa
    30481 acaatatcat tcatcaagca gagcaagatg cgcaacgctt gttggaagaa gctaaatata
    30541 aggcaaacga gattcttcgt caagcaactg ataatgctaa gaaagtcgct gttgaaacag
    30601 aagaattgaa gaacaagagc cgtgtcttcc accaacgtct caaatctaca attgagagtc
    30661 agttggctat tgttgaatct tcagattggg aagatattct ccgtccaaca gctacttatc
    30721 ttcaaactag tgatgaagcc tttaaagaag tggttagcga agtacttgga gaaccgattc
    30781 cagctccaat tgaagaagaa ccaattgata tgacacgtca gttctctcaa gcagaaatgg
    30841 aagaattaca agctcgtatt gaggtagccg ataaagaatt gtctgaattt gaagctcaga
    30901 ttaaacagga agtggaaact ccaactcctg tagtgagtcc tcaagttgaa gaagagcctc
    30961 tgctcatcca gttggcccaa tgtatgaaga accagaagta gctccagtgc atccgacagg
    31021 tccaacacca gctacagaaa ctgttgattc agcaccagga tttgaagcac cgcaagaatc
    31081 cgttacaatt ttataagaaa cgatgtgaga acaatatctt atccttatat ttccagcgag
    31141 caggagatgg tgtgagtcct gcattcccta acgataagat tatcctctca aaaactcaag
    31201 tctgaagcta gtaagatttg acgtttccca cgttacggga taagagggag aaagactaaa
    31261 tctttttccg aataaaggtg gtaccacgat tttcgtcctt tttggaagtc gtggttttta
    31321 atttgttatt atttataaag gagataccat gaaactcaaa gataccctta atcttgggaa
    31381 aacagctttc ccaatgcgtg caggccttcc gaccaaagag ccagtttggc aaaaggaatg
    31441 ggaagatgca aaactttacc aacgtcgtca agaactaaat gaagggaaac cgcattttgt
    31501 cttgcatgat ggccctccat acgcaaacgg aaatatccac gttggacatg ccatgaatca
    31561 tatctcaaaa gatatcatca ttcgttctaa gtctatgtca ggttttaacg caccttatat
    31621 cccaggttgg gatactcatg gtctgccaat cgagcaagtt ttggcaaagc aaggtgtgaa
    31681 gcgcaaagaa atggacttgg ttgagtactt gaaactctgt cgtgagtacg ctctttctca
    31741 agtttataaa caacgtgatg atttcaaacg cttggggatg tcaggtgact gggaaaattt
    31801 atatgtgact ttaactccag actatgaagc agcccaaatc cgtgtctttg gtgagatggc
    31861 taataagggt tatatctacc gtggtgccaa gccagtttac tggtcatggt catctgagtc
    31921 agcccttgcc gaagcggaaa ttgaatacca tgatttggtt tcgacttcac tttattatgc
    31981 caataaggta aaagatggca aaggtatcct agatacagat acttatatcg tagtctggac
    32041 aacaactcca tttaccatca cagcttctcg tggtttgact gttggtgcag atattgatta
    32101 cgttttggtt caacctgcta gtgaaactcg taagtttgtc gttgctgcag aattgttgac
    32161 tagcttgtct gagaaatttg gctgggctga tgttcaagtg ttggcaactt accgtggtca
    32221 agaactcaac cacatcgtaa cagaacaccc atgggataca gctgtagatg agttggtaat
    32281 ccttggtgat cacgttacca ctgactctgg tacaggtatc gtccatacag cccctggttt
    32341 tggtgaggac gactacaatg ttggtattgc taatggtctt gaagtcgcag tgactgttga
    32401 cgaacgcggt atcatgatgg cgaatgctgg tcctgagttt gaaggtcagt tctacgacaa
    32461 ggttgtacca actgttattg aaaaacttgg taacctcctt ctagcccaag aagaaatctc
    32521 tcactcatac ccatttgact ggcgtactaa gaaaccaatc atctggcgtg cagttccaca
    32581 atggtttgcc tcagtttcaa aattccgcca agaaatcttg gatgctattg ataaagttaa
    32641 attccataca gaatggggta aagtacgtct atataatatg attcgtgacc gtggtgactg
    32701 ggttatctct cgtcaacgtg cttggggtgt tccacttcca atcttctatg cagaagacgg
    32761 tacagctatc atgacagctg agactattga acatgtggcg caactctttg aagtacatgg
    32821 ttcaagcatt tggtgggaac gtgatgccaa agacctctta ccagaaggat ttacacatcc
    32881 aggttctcca aatggggagt tcaagaaaga aacagatatt atggatgtat ggtttgactc
    32941 aggttcatca tggaatggtg ttttggtaaa tcgtccaaat ctgacttatc cagctgacct
    33001 ttaccttgaa ggttctgacc aataccgtgg ttggttcaac tcatcactca ttacatctgt
    33061 tgccaaccat ggcgtagcac cttacaaaca aatcttgtca caaggtttta cccttgatgg
    33121 taaaggtgag aagatgtcta aatcacttgg aaataccatt gctccaagtg atgttgaaaa
    33181 acaatttggt gcagaaatct tgcgtctctg ggtaacaagt gttgactcaa gcaatgacgt
    33241 gcgtatctct atggatattt tgagccaagt ttctgaaact taccgtaaga ttcgtaacac
    33301 tcttcgtttc ttgattgcca atacatctga ctttaaccca gctcaagatg tagtcgctta
    33361 cgatgaactt cgttcagttg ataagtacat gactattcgc tttaaccagc ttgtcaagac
    33421 cattcgtgat gcctatgcgg actttgaatt cttgacaatc tacaaggcct tggtgaactt
    33481 tatcaacgtt gacttgtcag ccttctacct tgattttgcc aaagatgttg tttacattga
    33541 aggtgccaaa tcattggaac gccgtcaaat gcagactgtt ttctatgaca ttcttgtcaa
    33601 aatcaccaaa ctcttgacac caatccttcc tcacactgcg gaagaaatct ggtcatatct
    33661 tgagtttgaa gctgaagact tcgttcaatt gtcagaattg ccagaagctc aaacttttgc
    33721 taatcaagaa gaagtcttgg atacatgggc agccttcatg gacttccgtg gacaagctca
    33781 aaaagccttg gaagaagctc gtaatgcaaa agttatcggg aaatcgcttg aagcacactt
    33841 gacagtttat ccaaatgaag ttgtgaaaac tctactcgaa gcagtaaaca gcaatgtggc
    33901 tcaacttttg atcgtgtcag acttgaccat cgcagaaggg ccagctccag aagctgccct
    33961 tagcttcgaa gatgtagcct tcacagttga acgcgctgca ggtcaagtat gtgaccgttg
    34021 ccgtcgtatc gacccaacaa cagcagaacg cagctaccag gcagtcatct gtgaccactg
    34081 tgcaagcatc gtagaagaaa actttgcgga agcagtcgca gaaggatttg aagagaaata
    34141 aggttgaaaa gtctaggcaa aattcaattt gagaagaaaa gacaactaat tttatagtct
    34201 attaaacgca ttgtatcacg tttttgaata cctgatatga tgcgtttttt atttatttta
    34261 aaaatttgcg aggtatgact ttttatactc aacaagaatc aaagagaaac ttagcaagct
    34321 aacagtagta agataaaata ggaatttgat attagggat
//
LOCUS       DAVMEI010000007        32564 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVMEI010000007 DAVMEI010000000
VERSION     DAVMEI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.007"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            370..1533
                     /locus_tag="VYQ24_000276"
     CDS             370..1533
                     /locus_tag="VYQ24_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1967465.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1530..2207
                     /locus_tag="VYQ24_000277"
     CDS             1530..2207
                     /locus_tag="VYQ24_000277"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1967466.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2197..3051
                     /locus_tag="VYQ24_000278"
     CDS             2197..3051
                     /locus_tag="VYQ24_000278"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1967467.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3070..4137
                     /locus_tag="VYQ24_000279"
     CDS             3070..4137
                     /locus_tag="VYQ24_000279"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1967468.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4147..5313
                     /gene="aroC"
                     /locus_tag="VYQ24_000280"
     CDS             4147..5313
                     /gene="aroC"
                     /locus_tag="VYQ24_000280"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1967469.1"
                     /translation="MRYLTAGESHGPRLTAIIDGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKCLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5323..6426
                     /locus_tag="VYQ24_000281"
     CDS             5323..6426
                     /locus_tag="VYQ24_000281"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1967470.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6437..6775
                     /locus_tag="VYQ24_000282"
     CDS             6437..6775
                     /locus_tag="VYQ24_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1967471.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6867..8150
                     /gene="aroA"
                     /locus_tag="VYQ24_000283"
     CDS             6867..8150
                     /gene="aroA"
                     /locus_tag="VYQ24_000283"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1967472.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8143..8619
                     /locus_tag="VYQ24_000284"
     CDS             8143..8619
                     /locus_tag="VYQ24_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1967473.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8616..9464
                     /gene="pheA"
                     /locus_tag="VYQ24_000285"
     CDS             8616..9464
                     /gene="pheA"
                     /locus_tag="VYQ24_000285"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1967474.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSIHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVNYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9461..10735
                     /gene="psr"
                     /locus_tag="VYQ24_000286"
     CDS             9461..10735
                     /gene="psr"
                     /locus_tag="VYQ24_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1967475.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPSKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11014..11859
                     /locus_tag="VYQ24_000287"
     CDS             11014..11859
                     /locus_tag="VYQ24_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1967476.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLCYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11849..12952
                     /locus_tag="VYQ24_000288"
     CDS             11849..12952
                     /locus_tag="VYQ24_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1967477.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVGILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEEFLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12954..13940
                     /locus_tag="VYQ24_000289"
     CDS             12954..13940
                     /locus_tag="VYQ24_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1967478.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKIIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13956..15389
                     /locus_tag="VYQ24_000290"
     CDS             13956..15389
                     /locus_tag="VYQ24_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1967479.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15418..16452
                     /locus_tag="VYQ24_000291"
     CDS             15418..16452
                     /locus_tag="VYQ24_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1967480.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDVNDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16652..17389
                     /gene="mecA"
                     /locus_tag="VYQ24_000292"
     CDS             16652..17389
                     /gene="mecA"
                     /locus_tag="VYQ24_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1967481.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17540..18826
                     /locus_tag="VYQ24_000293"
     CDS             17540..18826
                     /locus_tag="VYQ24_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1967482.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQEFGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18828..19697
                     /locus_tag="VYQ24_000294"
     CDS             18828..19697
                     /locus_tag="VYQ24_000294"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1967483.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19773..19847
                     /locus_tag="VYQ24_000295"
                     /pseudo
     CDS             19773..19847
                     /locus_tag="VYQ24_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            19992..20930
                     /gene="msrB"
                     /locus_tag="VYQ24_000296"
     CDS             19992..20930
                     /gene="msrB"
                     /locus_tag="VYQ24_000296"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1967484.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20988..22712
                     /locus_tag="VYQ24_000297"
     CDS             20988..22712
                     /locus_tag="VYQ24_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967485.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VLVAILTIVIVGLSRLVNPFYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISVKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRYISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            22714..24465
                     /locus_tag="VYQ24_000298"
     CDS             22714..24465
                     /locus_tag="VYQ24_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967486.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKKSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGSA
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     TSDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRF
                     YPISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVI
                     AAAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATS
                     SIDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMD
                     RKGKYYQMQKAAAFSSE"
     gene            24554..24634
                     /locus_tag="VYQ24_000299"
                     /pseudo
     CDS             24554..24634
                     /locus_tag="VYQ24_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            25183..26544
                     /gene="trpE"
                     /locus_tag="VYQ24_000300"
     CDS             25183..26544
                     /gene="trpE"
                     /locus_tag="VYQ24_000300"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1967487.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPQKFEEMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVINPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            26541..27107
                     /locus_tag="VYQ24_000301"
     CDS             26541..27107
                     /locus_tag="VYQ24_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1967488.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            27118..28122
                     /gene="trpD"
                     /locus_tag="VYQ24_000302"
     CDS             27118..28122
                     /gene="trpD"
                     /locus_tag="VYQ24_000302"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1967489.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            28119..28886
                     /gene="trpC"
                     /locus_tag="VYQ24_000303"
     CDS             28119..28886
                     /gene="trpC"
                     /locus_tag="VYQ24_000303"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1967490.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENRAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAEDVAQRIKELQIDKG"
     gene            28873..29472
                     /locus_tag="VYQ24_000304"
     CDS             28873..29472
                     /locus_tag="VYQ24_000304"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1967491.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAV
                     LAKLIPADVKKVGVFVSPSQVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            29450..30673
                     /gene="trpB"
                     /locus_tag="VYQ24_000305"
     CDS             29450..30673
                     /gene="trpB"
                     /locus_tag="VYQ24_000305"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1967492.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            30666..31442
                     /locus_tag="VYQ24_000306"
     CDS             30666..31442
                     /locus_tag="VYQ24_000306"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1967493.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLGETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLANTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(31414..32073)
                     /locus_tag="VYQ24_000307"
     CDS             complement(31414..32073)
                     /locus_tag="VYQ24_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1967494.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLSKVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            32143..>32564
                     /locus_tag="VYQ24_000308"
     CDS             32143..>32564
                     /locus_tag="VYQ24_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967495.1"
                     /translation="MTIRFEEKVSTENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRVQNLYQKEGFEIVQMVEAPVR"
BASE COUNT         9586 a         6173 c         7551 g         9254 t
ORIGIN      
        1 atattgagtt tgtaacaaaa ggcttttcta taaaatcaaa ttttccagat acctattgaa
       61 gcagatttat ttcttcatct ttcttctact acctttgtca ctaaatttga cagacacata
      121 ttgctgattc aacctctcta cctataatct cacagttaac ctgaggctag tttcatagtt
      181 tgctatttga ttttcgttga taaaaagaaa tgctcctcca agacggaaga gcatttttta
      241 ttgtgattct tacacattta cttcctagtt ttgcaggaag tctagtatac cattttttaa
      301 aagaagagta aaaaagcaag ggtagtttct tttttgagaa ttgtctaaaa gtttgatata
      361 atggtagtta tgaatagaat aagagtcagc aaaagggttg aaaagaagct tgctaagggg
      421 ctagttttac tagaagccag tgatcttgag aatgtcaatc ttaaggatca ggaagtagag
      481 gtgcagggtc aggaaggaaa ctttcttggg actgcctacc tttctcagca aaacaagggc
      541 ttgggctggt ttgtcagcaa agacaaggtg gccttcaatc aaactttctt tgaaacgttg
      601 tttagaaaag ccaaagaaaa gagaaacgcc tactatcaag atgatttgac aactgccttt
      661 cgtctcttta atcaagaggg agatggcttt gggggtctga cagtggacct ttatggcgac
      721 tacgccgtct tttcttggta taactcttat gtttatcaga ttcgtcagac tatatcagaa
      781 gcctttagac aggttttccc tgaggtttta ggggcttatg agaaaatccg ctttaagggt
      841 tttgactatg aatctgccca tgtttatggt caagaagcac ctgacttttt cactgtttta
      901 gaaaatggtg tcctgtatca agtctttatg aatgatggct tgatgacagg aattttccta
      961 gaccagcatg aggttcgcgg tagtttagtt gacggcttgg ctatgggtaa atccttgctc
     1021 aatatgtttt cctacacagc ggctttttca gtagctgcgg ccatgggagg agctagccag
     1081 acaacttctg ttgatctagc caaacgttca cgagaattgt ctcaagcgca ttttcaggca
     1141 aatgggctca gcacagacga gcatcgtttt atagtcatgg atgtctttga gtatttcaaa
     1201 tatgccaaac gaaaagactt gacctacgat gtgattgtcc tagatccgcc tagctttgct
     1261 cggaataaaa aacaaacttt ctctgtggcc aaggattatc acaagttgat ttcccagagt
     1321 cttgagattt taaatccggg agggattatc attgccagta ccaatgctgc caatgtttcc
     1381 cgtcagaaat ttacagaaca aattgataaa ggctttgcag gaagaagtta ccagatttta
     1441 aacaaatatg gtcttccagc agattttgcc tataataaaa aagatgaaag tagtaattac
     1501 ctcaaggtga ttagtatgaa ggttagtaaa tgaaattaat cgtttcagta atgccaagaa
     1561 gtttagagga ggctcaggct ctggatgcca cgaggtacct ggatgccgac atcattgaat
     1621 ggcgtgccga ctatctgcct aaagaagcga ttttgcaggt agctccagcc atttttgaaa
     1681 aattcgcagg ccgtgagttg gttttcacgc tacgaactcg ctccgaaggt ggagaaatcg
     1741 acctttctcc agaagagtat atccatctaa tcaaggaagt tgcgcaattc tatcaaccag
     1801 actatattga ttttgagtac tatagctaca aggatgtttt tgaggaaatg ctggacttcc
     1861 caaatcttgt tttgagttac cataatttcc aagaaacacc tgagaacatg atggaaatct
     1921 tgtcagagtt gacgatccta aatccaaaac ttgttaaggt tgcggtgatg gctcacacgg
     1981 agcaggatgt cttagacttg atgaactata cacgaggctt taaaaccctc aatcctgaac
     2041 aggaatatgt gaccatttct atgggtaagg tgggcaaggt ctctcgtatc actgcggatg
     2101 tgactggttc gagttggtct tttgccagtc tggatgaggt tagtgcccct gggcagattt
     2161 ctctagctag catgaaaaaa atcagggaga ttttggatga agcttgatgg ctatacacgt
     2221 ttagctgcag ttgttgccaa tcctattaag cattctattt ctcccttcat ccacaataga
     2281 gcctttgagg cgacagctac caacggtgct tatgtggctt gggagattga agcgagtgac
     2341 ttggcagaaa cagtggccaa tattcgtcgc taccagatgt ttggcatcaa tctgtccatg
     2401 ccctataagg agcaggtgat tccttatttg gataagctga gcgatgaagc gcgcttgatt
     2461 ggtgcggtta atacggttgt caatgagaat ggcaatttaa ttggatataa tacagatggc
     2521 aagggatttt ttaagtgctt gccttctttt acaatttcag gtaaaaagat gaccctgctg
     2581 ggtgcaggtg gtgcggctaa atcaatcttg gcacaggcta ttttggatgg cgtcagtcag
     2641 atttcggtct ttgttcgttc cgtttctatg gaaaaaacaa gaccttacct agacaagtta
     2701 caggagcaga caggttttaa agtggatttg tgtgctttag agtatgtttc tgaactgcaa
     2761 gcaaggattg ccgagtcgga tttgctagtt aatgccacca gtgtgggcat ggatggccaa
     2821 tcctctcctg ttcctgaaaa catagtctta ccagaaactc tcttagttgc agatatcata
     2881 taccaaccct ttgaaacacc gtttttgaaa tgggctagaa gtcagggcaa tccagcagtc
     2941 aatggtctgg gaatgttgct ctatcaagct gcagaagctt ttcaactgtg gacaggcaag
     3001 gaaatgccga cagaagagat ttggcagtct ttaacagaaa aataccaata aagaaaggga
     3061 gactctccta tgaaaatcag aatcgatatt cctcatcatc cttatgatat tcagattgaa
     3121 aaaggttgta tggcccaggc tggtcagtgg ttgcgagaac tctggcaacc gcaaaaggta
     3181 gtcattgtga cagataacca tgtagcctct ctctatgcag agaaggtcaa gctcagccta
     3241 gaagatgctg gttttcaggt agctgttttt gatttcttag aaggtgaaga aagaaagaat
     3301 ttaactactg ttcagaaagt ctatgaattt ctagtcaagc aaggtctgac tcgtagcgat
     3361 ggaatcgttg ctcttggtgg tggcgttgtt ggggacctgg ctggatttgt agcctctacc
     3421 tatatgcggg gtattcactt tgttcagatt ccgactagtt tgacagctca ggttgattct
     3481 tctatcggtg gaaagacagg cgttaacaca ccatttgcta aaaatatggt ggggaccttt
     3541 gcccaaccag atggggttct gattgatcca cttgttcttg aaaccctcgg aaaaagagag
     3601 ttgattgaag ggatgggtga agttatcaag tatggcttga ttgaggatcc agaactgtgg
     3661 gctctcttga cgggactgaa tggttctgtt gagagtattt tggaacatgc agagaccttg
     3721 attgaacatt cttgtcaggt gaagcgcaag atggtggttg aagatgagtt ggacaatggt
     3781 attcgtcttt acctcaattt tggccacact attggccatg ccatcgaagc gactgccggt
     3841 tatggcaagg tcatgcatgg agaggctgtt gccatgggaa tggtacagat ttccaagatt
     3901 gctgaggaaa aaggcctcat gccagctggc attacccaat ctatcacaga gatgtgtcag
     3961 aaattcggct tgcctgttga ctatgaaaat tgggaagttg acaagcttta tcaggctctt
     4021 actcatgaca agaaagcgcg tggcaacacc ttgaaattgg tcttggtgcc agagcttggt
     4081 tcagcgatca ttcacccagt ttctctggaa gagatgaaag actacttggt aaaataagga
     4141 gagtgtatga gatatttaac tgcaggagaa tcacacggcc cccgtctaac agctattatt
     4201 gatggaattc cagctggact tccattgaca gctgaggata tcaatgagga ccttagacgc
     4261 cgtcagggtg gctacggtcg tggtggtcgt atgaagattg agaatgacca ggttgtcttt
     4321 acttcgggcg ttcgccacgg gaagacgaca ggggcgccta ttactatgga tgtcatcaat
     4381 aaggaccacc agaaatggct ggacatcatg tctgcggagg acattgaaga ccgtcttaaa
     4441 agcaagcgga aaattactca tcctcgccca ggtcatgccg atttggttgg ggggattaag
     4501 taccgttttg atgatttgcg aaattctttg gagcgttcat cagctcgtga aaccaccatg
     4561 cgggtggcag ttggtgcagt agccaaatgc ctcttggctg agctggatat ggagattgcc
     4621 aaccatgtcg tggtctttgg tggcaaggaa atcgatgttc ctgaaaatct gacagtcgct
     4681 gaaattaagc aaagagctgc ccagtctgaa gtttctattg tcaaccaaga acgagaacag
     4741 gaaatcaagg actatattga ccaaatcaaa cgtgatggtg ataccatcgg tggggttgtg
     4801 gagacagtcg tcggaggcgt tccagttggt cttggttcct atgtccaatg ggatagaaaa
     4861 ttggatgcaa gattggctca agctgttgtc tctatcaatg cctttaaagg ggtggaattt
     4921 ggtcttggct ttgaggctgg ttatcgtaaa ggcagccaag ttatggatga aattctctgg
     4981 tctaaagaag acggttatac tcgccgtacc aataatctag gtggttttga aggtggtatg
     5041 actaatgggc aacccatcgt tgttcgtggg gtcatgaaac ccattcctac tctttataaa
     5101 cctcttatga gtgtggatat cgaaacccac gaaccttaca aggcaaccgt ggagagaagt
     5161 gatccgactg ctcttccagc tgcaggaatg gtcatggaag cagttgtagc aacggttctg
     5221 gcgcaagaaa tcctcgaaaa attctcatca gataatcttg aggaactaaa agaagcggta
     5281 gccaaacacc gagactatac aaagaactat taaggagttc ctatggcaaa aacaatctat
     5341 atcgcaggtc ttgggttgat tggagcctct atggcacttg gtatcaaacg cgatcatcca
     5401 gattatgaaa ttttaggtta taatcgtagt caagcttcga gagatatcgc cttgaaagaa
     5461 ggtatgattg accgtgcaac ggatgatttt gctagttttg ctcctttggc agatgtcatt
     5521 atcctcagct tgccaatcaa acaaactatt gctttcatta aggagttggc caatttggat
     5581 ttgcgagaag gcgttattat ttcagatgct ggttcgacca agtcaaccat tgtggatgcg
     5641 gcggagcagt atttggctgg caagtctgtt cgctttgtcg gggcccatcc catggctggt
     5701 agtcacaaga caggggctgc ttcggcagat gtcaatcttt ttgaaaatgc ctattatatc
     5761 tttacacctt caagcctgac aagtcaggac acgcttaagg aaatgaagga tctactttca
     5821 ggtcttcatg ctcgttttat cgagattgat gccaaggagc atgatcgtgt cacttctcag
     5881 attagccatt ttcctcatat tttggcttct agtctcatgg agcagactgc ggtctatgct
     5941 caagagcatg agatggcaag gcgctttgcg gcaggtggtt ttcgagatat gacccgaatt
     6001 gcggaaagcg agccaggaat gtggacctcc attctcttgt ccaatagcga gatcattctg
     6061 gatagaattg aggatttcaa ggaacgtttg gaagcgattg gtcaggccat tagtaaggga
     6121 gatgaagagc aaatttggaa cttttttaac caagcgcgtg aacaacgcca gaccatggaa
     6181 atccataagc gtggtggtgt ggatagctct tacgacctct atgttgacgt tcctgatgaa
     6241 gaagatgtca tcctgcggat tttggaactg ctacgtggaa cttccttggt taacatccac
     6301 atcaacgagg aaaatcgtga ggatattcac ggaatcctac aaatttcatt taaaaatgct
     6361 caagacttgg aaagagctga gcatctcata acagaaaata ccgactatac agtcgtcatc
     6421 aaataaggag aagaacatgt caaatattta cgatagtgca aacgaactca gtcgcggtct
     6481 acgcggatta ccagaataca aggctgttaa agcagctaaa gatgcgattg cagcagatgc
     6541 tgaggcaagc aaaatcttta cagaatatct tgcctttcaa gaggaaattc aaaaactagc
     6601 acagacaggt caaatgccag atgcgtcctt ccaagctaag atggaaggct ttggtaaaca
     6661 gattcaagga aatagcctct tgtcagaatt ctttaccaag caacaacaat tggccattta
     6721 cctttctgac attgaaaaaa ttgtttttga accagtttca gaattgctga aataagaaaa
     6781 gaacgatcct aaagtcagga atttttaagg aatttctggc tttttatgat aaaataggta
     6841 aaagtattgc aagtatgagg tccagtatga aactaaaaac aaacattcgc catttacatg
     6901 gtagtatccg cgtcccaggt gacaagtcta tcagccaccg ttccattatc tttggaagtt
     6961 tggctgaggg tgagaccaag gtttatgata ttctgcgagg cgaagacgtt ctttcgacca
     7021 tgcaggtttt tcgtgacctt ggtgttgaaa ttgaggataa agatggggtt attaccattc
     7081 aaggtgtagg catggctggc ttaaaagcgc cgcaaaatgc ccttaatatg ggaaattctg
     7141 gcacctcgat tcgcctgatt tcaggtgtcc ttgctggtgc agatttcgaa gtagagatgt
     7201 ttggagatga tagtctttcc aaacgtccta tggaccgtgt gacccttcca ctgaaaaaaa
     7261 tgggcgtcag catttcaggg caaactgaac gagacttgcc tccccttcgc ttaaaaggga
     7321 cgaaaaacct aagacctatt cattatgagt tgccaattgc ctctgcccaa gtcaagtcag
     7381 ccttgatgtt tgcagcctta caagctaagg gggagtcagt tattatcgaa aaagagtgca
     7441 cccgtaatca tactgaagat atgttgaaac aatttggtgg tcatttaagt gtggacggta
     7501 agaaaatcac agtccaaggg ccacaaaaat tgacaggaca gaaggtggtc gtaccaggag
     7561 atatttccag tgcagccttt tggttagtcg caggtttgat tgttccaaat tctcgtctag
     7621 tgctgcagaa tgtggggatc aacgaaactc gcaccggtat tattgatgtc attcgtgcca
     7681 tgggtggaaa attggaaata actgaaatcg atccagtcgc taaatcttct accttgactg
     7741 ttgagtcttc tgacttgaaa ggaacagaga ttggtggcgc tttgattcca cgattgattg
     7801 atgaattgcc cattattgcc ctgcttgcga ctcaagccca aggtgtaaca gttatcaagg
     7861 atgctgagga gctcaaggtc aaggaaacag accgtattca ggttgtggca gacgccttaa
     7921 atagtatggg agcagatatt actcctacgg cagatgggat gattatcaaa ggaaaatcag
     7981 ctcttcacgg tgctagagtc aatacgtttg gtgaccaccg tatcggcatg atgacagcta
     8041 tcgcagccct attggttgca gatggagagg tggagcttga ccgtgcagaa gccatcaata
     8101 ccagctatcc tagtttcttt gatgatttgg agagcttgat tcatggctaa ggtgttatta
     8161 gggtttatgg gggctggaaa atcgactatt gcaagaggct tggaccctaa ttaccttgat
     8221 atggatgctc tgattgagaa gcgcctaggt atgtccattg cgaatttttt cgctgaaaag
     8281 ggagaagcag cctttcgtca ggtagaatca gaagtcctag ctgatttact acaaacagac
     8341 caagtcgtgt caactggagg aggagtggtt atttctcaga gaaatcgtga cttactcaag
     8401 actaatacag ataacatcta cctgaaagca gattttgaaa ccctctacct acgtatcgta
     8461 gctgataagg acaatcagcg accgcttttt ctaaataata gcaaggaaga actagtagct
     8521 atttttcaag aaagacaggc ttggtatgag gaagtggcta gtcgggtttt ggatgtgacc
     8581 aagctaagcc cagaggaaat tatagaggaa ctaagatgaa aattgcttat ctaggtccca
     8641 agggatcatt ttcacaccac gttgtgcaga cagcttttcc tcatgaggaa ttgcaggctt
     8701 ttgccaacat tacagatgtc atcaaggctt atgagcaagg attagtggac tattctgtgg
     8761 tgccagttga aaattctatt gagggtagta ttcatgaaac cttggactat ctttttcatc
     8821 aggctcacat ccaagcagta gcagaaatcg ttcagcctat tcatcagcag ttgatggtgg
     8881 ttccaggcca tactaagatt gaaaagattt tttcacatcc acaggccttg gctcaaggaa
     8941 agaaattcat cgatgaacaa tatccagagg ctcaaatcga ggtaacagct agtacagctt
     9001 atgcggcccg ttttatttcc gaacatccag accagccctt tgcagcagtt gcacctagaa
     9061 gttctgctga agaatatgga ttggaactga ttgccgagga tattcaggaa atggaagcca
     9121 atttcacacg tttctgggtt ctaggagctg aaaaacctag tattcccttg caagcacaaa
     9181 ctgaaaagat gagtttggcc ttgacattac ctgacaacct tccaggtgca ctttataagg
     9241 ccctgtcgac ctttgcttgg cgaggaattg acttgacaaa aattgaaagt cgtccactca
     9301 agacagcact gggtgaatac tttttcatta tcgatgtgaa ttataccgat aaggacttgg
     9361 tccactttgc ccaaaaagaa ttagaagcga ttggaatcca gtataaaatt ctgggtgcct
     9421 atcctattta tccaatatca gaccatggaa aggagagaag atgagtaaag aaaatccctt
     9481 aagtcatcat gagcagttgc gttatgatta tttgctaaaa aatattcact atctcaatga
     9541 gagagaaaaa aatgagtttg cctatttgca agaaaagcta actcttgcta ggggaaatag
     9601 tagctctagc ttggaacaag aaagagaaga gcaggttgac ttaccaagct atgcgaaccg
     9661 gagtcgctca caatccaaat cacaagcact ctctttccct tcaaaaaaga aaagacggaa
     9721 gctccgtctt aaacgaattt ttatggtgat tttttcactt ttggtctgtg tggctttggc
     9781 catggtattc atgtttttgc gtggttacca agatgctagc gcaaagaaaa ctgctgatgc
     9841 ccgggcagct caagtagaag tctttaatgg tcaggacact agagatggag ttaatatttt
     9901 aatcatgggt actgatggtc gaatcggcca gaacagtgtt gagacacgaa ctgactctat
     9961 tatggtatta aatgtcgggg gctcagataa gaaaatgaag ctagtcagtt tcatgcgtga
    10021 taatttggtc tatatagatg gttatagtca agtgattaat ggtagaaaac agacagataa
    10081 caagttaaac gtagcctacg agttaggaga acaagagggg caaaaagggg cagaaatggt
    10141 tcgccaagtc ttgaaagata attttgactt ggacattaag tactacgcct tggttgattt
    10201 ccaggccttt gccacagcga ttgacacgct tttccctgat ggggtgacga ttgatgctca
    10261 attttcaaca ttgaatgggc gtcccctaac agaagctaca gtcggagatg atttatacgc
    10321 taccgagact gagtctccaa cccaaaccat taaagtcgga aaacagcaga tgaatggctc
    10381 gaccctgctc aattatgctc gtttccgtga tgatgatgag gcagattacg gccgtacaaa
    10441 aagacagcaa caagttttaa cagcaatttt agagcaaatt aaagatccca ctaaactttt
    10501 cactggttct gaagctcttg gaaaggtttt tgctatgacc tcaaccaacg taccctatac
    10561 tttcctccta acaaacggtt tatctgtttt ggatggagca aaaaatggta ttgaaaaatt
    10621 gacgattcca gaactgggtg actgggtaga tgcctatgat gtttatggag gcttgggctt
    10681 gctggttgat caaaacaaat atcaaaccaa gctcgctcaa atgggcttga gataagataa
    10741 tactcaatga aaatcaaagt gcaaactagg aagctagccg caggctgcta aaaacacagt
    10801 tttgaggttg tagataagac tgacgaagtc agttacctat acctacggca aggtgaagct
    10861 gacgtggttt gaagagattt tcgaagagta taagacagaa aaatcaaagt ttgatagcta
    10921 tttggatagc agtcaggctt tgatttttta cagtctgcaa tagattttca ccccttgttt
    10981 gtggtataat gaaacgatac gatagaaaga ataatgaaca atatgactga tttaaaagca
    11041 attcaggctc gtagtctgga gatggctgaa tattttgtgg ccttttgtaa agaacatgat
    11101 ttgctctgtt atctctgtgg cggaggcgct attggtgccc ttcgaaacaa gggatttatt
    11161 ccttgggacg acgacctaga cttttttatg cctcgtaaag attatgagaa attagcagaa
    11221 ttatggcctc gttatgcaga tgaacgttat ttcttgtcaa agagtcacaa ggattttgtt
    11281 gatcgtaatc tttttattac cattcgtgac aaggaaacca cctgtatcaa gccttatcag
    11341 caggatttgg atttgccaca tggtctggcc ttggatgttt tgcctttgga ttattatccg
    11401 aaaaatccag ctgagcggaa aaaacaggtt cgttgggcct tgatttattc actcttttgt
    11461 gcgcaaacta ttccagaaaa gcatggtgct ctcatgaaat ggggaagtcg cattttactg
    11521 ggtttgactc caaaatctct ctgttatcgc atctggaaaa aagctgagaa agaaatgact
    11581 aagtatgatt tggctgattg tgatggcatt acagaattat gctcaggtcc tggctacatg
    11641 agaaacaagt acccaatcac atcttttgaa gacaatcttt tcttgccatt tgaaggaaca
    11701 gagatgccta ttccaatcgg ctatgatgtc tatctcagaa ctgcttttgg ggattatatg
    11761 acgcctccac cagcagacaa gcaggtaccg catcatgatg ctgtcatcgc tgatatggat
    11821 aagtcttata cagaatacaa gggagaatat ggtggctaag aaaaaaatct tattttttat
    11881 gtggtctttt tctcttggag gtggtgcaga gaagattcta tcaaccattg tttcaaatct
    11941 ggatctagaa aagtatgata ttgatattct tgaaatggag cactttgaca agggatatga
    12001 atctgttcca aagcatgtag gcattttaaa atcccttcaa gattatcgcc aaaccagatg
    12061 gttacgagct tttttgtgga gaatgagaat ttattttcca agactgactc gtcgtttgct
    12121 tgtaaaagat gattatgatg ttgaagtttc ttttaccatt atgaatccac cactgttgtt
    12181 ctctaaaaga agagaagtca agaagatatc ttggattcat ggaagtattg aagaatttct
    12241 taaggatagc tctaaaagag aatcacatag aagccagttg gatgctgcga atacaattgt
    12301 agggatttca aaaaagacca gcaattctat caaggaagtt tatccagatt atgcttctaa
    12361 attacagaca atctacaatg gatatgattt tcagactatt ctagaaaaat ctcaagagaa
    12421 gatcgatatc gagattgctc ctcaaagtat ctgtactatc ggacggattg aggaaaataa
    12481 gggttctgac cgtgtagtgg aagtgatacg attattacac caagagggaa aaaactatca
    12541 tctctatttt atcggggctg gtgatatgga agaggaactg aaaaaacgag tcaaagagta
    12601 tgggattgag gactatgtac atttccttgg ttatcaaaaa aatccttatc agtatctatc
    12661 tcagacgaaa gttctcttgt ctatgtctaa acaagaaggt tttcctggag tgtatgtgga
    12721 ggccttgagt ctgggactcc cttttatctc tacggacgtt ggaggggctg aggaattatc
    12781 ccaagaagga cgatttggac aaatcattga gagcaatcaa gaggcagctc aggcgattac
    12841 taattacatg acttctgcct caaactttga tgtcgatgag gctagccaat tcattcaaca
    12901 atttacaatt acaaaacaaa tcgaacaagt agaaaaacta ttagaggagt agcatggaaa
    12961 ctgcattaat tagtgtgatt gtgccagtct ataatgtggc gcagtaccta gaaaaatcga
    13021 tagcttccat tcagaagcag acctatcaaa atctggaaat tattcttgtt gatgatggtg
    13081 caacagatga aagtggtcgc ttgtgtgatt caatcgctga acaagatgac agggtgtcag
    13141 tgcttcataa aaagaacgaa ggattgtcgc aagcacgaaa tgatgggatg aagcaggctc
    13201 acggggatta tctgattttt attgactcag atgattatat ccatccagaa atgattcaga
    13261 gcttatatga gcaattagtt caagaagatg cggatgtttc aagctgtggt gtcatgaatg
    13321 tctatgctaa tgatgaaagc ccacagtcag ccaatcagga tgactatttt gtctgtgatt
    13381 ctcaaacatt tctaaaggaa tacctcatag gtgaaaaaat acctgggacg atttgcaata
    13441 agataatcaa gagacagatt gcaactgccc tatcctttcc taaggggttg atttacgaag
    13501 atgcctatta ccattttgat ttaatcaagt tggccaagaa gtatgtggtt aatactaaac
    13561 cctattatta ctatttccat agaggggata gtattacgac caaaccctat gcagagaagg
    13621 atttagccta tattgatatc taccaaaagt tttataatga agttgtgaaa aactatcctg
    13681 acttgaaaga ggtcgctttt ttcagattgg cctatgccca cttctttatt ctggataaga
    13741 tgttgctaga tgatcagtat aaacagtttg aagcctattc tcagattcat cgttttttaa
    13801 aaggccatgc ctttgctatt tctaggaatc caattttccg taaggggaga agaattagtg
    13861 ctttggccct attcataaat atttccttat atcgattctt attactgaaa aatattgaaa
    13921 aatctaaaaa attacattag aacgggggtg accaagtgat tgatgggaaa cgattattat
    13981 ttagtttgac catagtcagt tatgccttga cgctagtaag tggaattgtg tatctgttta
    14041 ataataataa tgttagctta ctttctactt tattgttctt gttggttagt agcttaattg
    14101 cttgttggaa tgatatcaag tattacttaa tccattttat tttctattta accatttttg
    14161 tatttctggt atcaagaccg accattgatt attttaggga tggtgctttg gatacctatc
    14221 atccaatagc ctatcgtttt gcctttatag ttgtcatggt ttcgattctg ggcttgacca
    14281 caggaggcat tctggctcgt tacttcatag ctaggaagaa aataaaagta gcaaatatag
    14341 gaaattctct aaaagaggtt tatatcaagc ggttacgctt tgtatcacta ggagtttttc
    14401 ttctaaccta tcctttctat ttcattcggt tatttgaacg gctcttgtat cgtttgcaga
    14461 cttcctacta tgcctactat gcaaattttg aaagtaaact gccttatttt acctacattt
    14521 tgtctacctt tacggtctat gcaatgtgta tgtatctggc aaccaagcca aagaaattgc
    14581 aggccacagc agtgcttgtc tcctttattg cagctaatac tattcatttg gcaattggga
    14641 cacgaaatcc ctttatttta agtattttat ttgcttttgt ttattacttt atgcgggagc
    14701 aaactgaaaa aggaaaatgg attgggttta aagaaaagtt agcgattttt gtaggttctc
    14761 ctattctcat gttagcgatg ggagtactca attatgtacg ggataatgtc caagtttctc
    14821 atacaggttt ctgggatatc ttacttgact ttatctataa acaagggact agttttggtg
    14881 ttctggctcg aggttttcta tttaacagta gcctccctta ccgagatttc cgtaatttta
    14941 cttttggtcc tgttcttgat tattttgcaa gggggagttt gggagccatt ttcggaggaa
    15001 aagcctttga acatacaacc aatagtgtgg aactagctat tgatagtaat agttatgccc
    15061 acaatctatc ctatcttgtc ttgaacaagg aatacttgaa agggcatggt atcggaagta
    15121 gttatatcat ggagttgtat accgactatg gtatgattgg agtctttctg cttagtttct
    15181 tactcggcgt attatttata gccatgctgc aagtagccta tcgctcaagg acaatcctat
    15241 ttgctttatc cctactcatc ttgaataatc tattctttat gccaagaagc agcttttcag
    15301 aaagtttctt caatttattt acaatgcaat tctggggaat tgttcttgtg attatatttg
    15361 tagcaaaaat gcttacaaag gaaaactagt atctactaaa caaaggagaa aaaaatcatg
    15421 tttgaacatt attcagtagc tgatttgttt gcaaatcttt ataaaaagcg aaaagcaaat
    15481 attttagctc tcatcgcttt atttgctctc attgctgtac catttacaat taaagcagtt
    15541 aggaataaaa atactgtcaa agatacaaca agttattcaa cttatcttat ctataaaatc
    15601 actcctccaa aagagtcggc caaaacgatt ttgaatcatc aaattggtgg ttatagtgat
    15661 ttttatggga aattgattga tggtaatttg aatggagctt atcttttcaa tgatgtagaa
    15721 cccagtgagt tgaaaaaaat tgccagtgaa ttagacacga cagaaacaac cttgaaaaat
    15781 tcaacgaatg actattggtg gaaaaaattg accgtctact atatgattga cgatgcaggg
    15841 gttggtgtga aaattttgac atcaagtaaa gatgtcaatg acttgttaga gaaaaaaatt
    15901 gatgggttga ttgagaaatt taaacatgcc tatgcaaatg tgaaaattga aaaactggaa
    15961 accatcaact ctaaagaatt gaacgcaaat ggtgaaacag cgcttggctt aaatgtgaaa
    16021 aatctgattc ttcgtttagt tgttattgga gtggtttgtg tgattttggt tgtgatggga
    16081 aatgtgttag tttatctctt taatccaaca atcaatagag taggtgattt ttctcagtat
    16141 caaattgatt ttgtaacaga gattacaaca attgctaacc tagcagatgt tttgtcatac
    16201 aaaaatactg gacaggaatt gaccatcgtt agctcaaata aagctatcct agataaattg
    16261 aaacagagtc aagaagcttt aaaaggaatg cattttgtag atttgcagga tgtatcatct
    16321 cttttggaaa gagatacagt ccttcttgtt gaagagtacg gagtgactcg ttataagaaa
    16381 tttgagcaaa gtcttcaaat tctcagaaac ttaaatcgtt ctatcctcgg tgtagcaacc
    16441 tttaaattat aagtattctg atgtatgagg tcttcaaaat cggctaagag ctgattttga
    16501 aggctttttc tatctgtctg cagaaatttt cttttggagt ctcctataga aagaccctag
    16561 ggaaaatcta tccgtataga ggagccttct tttgataaaa tcgtaaaaat ctagtataat
    16621 agatagaact gaaagtatga ggttactagc aatgaaaatg aaacaaatta gtgatacaac
    16681 tttaaaaatc acgatgtctt tagaggattt gatggatcgt ggaatggaga ttgctgactt
    16741 tctcgttcct caagaaaaaa cagaagagtt cttttatgct atcttggatg agctagagat
    16801 gcctgatagc tttctggata caggcatgtt gagcttccgt gtgactccaa aacctgataa
    16861 ggtagatgtc tttgtgacca agtcaaagat tgaccaaaat ctagattttg aagacttatc
    16921 ggatttgcca gatatggaag aattggctca aatgtctcca gatgaattta tcaaaaccct
    16981 ggaaaaaagc atcgcagaca aaaccaagga tgatatcgaa gccattcaat ctcttgagca
    17041 agttgaagcc aaggaagaag agcaagagca ggctgaacaa gaagctgaga gtaagaaaga
    17101 accttacatc tactacatcc tttcttttgc taagttggct gacttggtag tttttgccaa
    17161 gacagtgact tttgaaatgg aaacttctga actctacaaa atgaacgagc gctattattt
    17221 gaccatttta gtggatattg aaaatcatcc aagcccatat ccagcttggc tgttggcccg
    17281 tatgcgcgag tttgcagacg atagtgatat cagtcgctca gtcttacaag agtatggtca
    17341 agtcttgatg agtcacgatg cagtgctcaa tctgcaaaaa atcggctaat cctttctgga
    17401 aagctatttt cagattttaa gaagagcgaa tcgtctgatt cgttttttct ttttatactg
    17461 aaatagtgat ttactataat aggaattttc acaaaattct gttataatgg ctatattaga
    17521 aaatttcgag gagataaaga tgacagttaa aattgcttta ctaggatttg gtaccgttgc
    17581 aagtggtgtg cctttcctcc taaaggaaaa tggaggaaaa atcaatcaat cagcacattc
    17641 agatatcaaa gttgctaagg tattggtcaa ggatgaagat gaaaaaaatc gcttgcttgc
    17701 agcagggaat gactttaact ttgtaaccaa tgtggatgat attttatcag accaggatat
    17761 tactatcgta gtggaattga tggggcgtat tgagcctgct aaaaccttta tcactcgtgc
    17821 cttggaagct ggaaaacacg ttgttactgc taacaaggac cttttagctg tccatggcgc
    17881 agaattgcta gaaatcgctc aagctaacaa ggtagcactt tactacgaag cagcagttgc
    17941 tggtgggatt ccaattcttc gtactttagc aaattccttg gcttctgata aaattacgcg
    18001 cgtgcttgga gtagtcaacg gaacttccaa cttcatggtg accaagatgg tggaagaagg
    18061 ctggtcttac gatgatgctc ttgcggaagc acaacgtcta ggatttgcag aaagcgatcc
    18121 gacgaatgac gtagatggga ttgatgcagc ctacaagatg gttattttga gccaatttgc
    18181 ctttggcatg aagattgcct ttgatgatgt agcccacaag ggaatccgca atatcacacc
    18241 agaagacgta gctgtagctc aagagtttgg ttacgtagtg aaattggttg gttctattga
    18301 ggaaacttct tcaggtattg ctgcagaagt gactccaacc ttcctaccta aagcgcaccc
    18361 acttgctagt gtgaatggcg taatgaacgc tgtctttgta gaatctatcg gtattggtga
    18421 gtctatgtac tacggaccag gtgcgggtca aaaaccaact gcaacaagtg ttgtagctga
    18481 tattgtccgt atcgttcgtc gtttgaatga tggtactatt ggcaaagact tcaacgaata
    18541 tagccgtgac ttggtcttgg caaatcctga agatgtcaaa gcaaactact atttctcaat
    18601 cttggctcta gactcaaaag gtcaggtctt gaagttggct gaaatcttca atgctcaaga
    18661 tatttccttt aagcaaatcc ttcaagatgg caaagagggt gacaaggcgc gtgtcgttat
    18721 catcacacac aagattaata aagcccagct tgaaaatgtc tcagctgaat tgaagaaggt
    18781 ttcagaattc gacctcttga ataccttcaa ggtgctagga gaataagatg aagattattg
    18841 tacctgcaac cagtgccaat atcgggccag gttttgactc ggtcggtgta gctgtaacca
    18901 agtatcttca aattgaggtc tgcgaagaac gagatgagtg gctgattgaa caccagattg
    18961 gcaaatggat tccacatgat gagcgtaatc tcttgctcaa aatcgctttg caaattgtac
    19021 cagacttgca accaagacgc ttgaaaatga ccagtgatgt ccctttggcg cgcggtttgg
    19081 gttcttccag ctcggttatc gttgctggga ttgaactagc caaccaactg ggtcaactca
    19141 acttatcaga ccatgaaaaa ttgcagttag cgaccaagat tgaagggcat cctgacaatg
    19201 tggctccagc catttatggt aatctcgtta ttgcaagttc tgttgaaggg caagtctctg
    19261 ctatcgtagc agactttcca gagtgtgatt ttctagctta cattccaaac tatgaattac
    19321 gtactcgcga cagccgtagt gtcttgccta aaaaattgtc ttataaggaa gctgttgctg
    19381 caagttctat cgccaatgta gcggttgctg ccttgttggc aggagacatg gtgaccgctg
    19441 ggcaagcaat cgagggagac ctcttccatg agcgctatcg tcaggacttg gtaagagaat
    19501 ttgcgatgat taagcaagtg accaaagaaa atggggccta tgcaacctac ctttctggtg
    19561 ctgggccgac agttatggtt ctggcttctc atgacaagat gccaacaatt aaggcagaat
    19621 tggaaaagca acctttcaaa ggaaaactgc atgacttgag agttgatacc caaggtgtcc
    19681 gtgtagaagc aaaataaaga atagaagata ggatggggaa actcttgacc agagggtttc
    19741 atatcctttt tgtgaaaaga agtttatact caatgaaaat caaagagcaa actaggaagc
    19801 tagccgcagg ctgctcaaaa cagtgttttg aggttgcaga tagaactgac gaagtcagct
    19861 caagacactg ttttgaggtt gcagatagaa ctgacgaagt cagtaacata cctacggtaa
    19921 ggtgacgctg acgtggtttg aagagatttt cgaagagtat tagttaaaaa cgtgataaag
    19981 gagaaataaa aatggcagaa atttatctag caggtggttg tttttggggc ctagaggaat
    20041 atttttcacg catttctgga gtgctagaaa ccagtgttgg ctacgctaat ggtcaagtcg
    20101 aaacgaccaa ttaccagttg ctcaaggaaa cagaccatgc agaaacggtc caagtgattt
    20161 acgatgagaa ggaagtgtca ctcagagaga ttttacttta ttatttccga gttatcgatc
    20221 ctctatctat caatcaacaa gggaatgacc gtggtcgcca atatcgaact gggatttatt
    20281 atcaggatga agcagatttg ccagctatct acacagtggt gcaggagcag gaacgcatgc
    20341 tgggtcgaaa gattgcagta gaagtggagc aattacgcca ctacattctg gctgaagact
    20401 accaccaaga ctatctcagg aagaatcctt caggttactg tcatatcgat gtgaccgatg
    20461 ctgataagcc attgattgat gcagcaaact atgaaaagcc tagtcaagag gtgttgaagg
    20521 ccagtctatc tgaagagtct tatcgtgtca cacaagaagc tgctacagag gctccattta
    20581 ccaatgccta tgaccaaacc tttgaagagg ggatttatgt agatattacg acaggtgagc
    20641 cactcttttt tgccaaggat aagtttgctt caggttgtgg ttggccaagt tttagccgtc
    20701 cgatttccaa agagttgatt cattattaca aggatctgag ccatggaatg gagcgaattg
    20761 aagttcgttc tcgttcaggc agtgctcact tgggtcatgt tttcacagat ggaccgcggg
    20821 agttaggcgg cctccgttac tgtatcaatt ctgcttcttt acgctttgtg gccaaggatg
    20881 agatggaaaa agcaggatat ggctatctat tgccttactt aaacaaataa aaaagagagt
    20941 ggggcttccc actttcttca tttctagaat atgaatagaa gggatttatg aaacacctat
    21001 tatcttactt caaaccctac atcaaggaat caattttagc ccccttgttc aagctgttag
    21061 aagctgtttt tgagctcttg gttcccatgg tgattgctgg gattgttgac caatctttac
    21121 ctcagggaga tcaaggtcat ctctggatgc agattggcct gctccttatc tttgcagtaa
    21181 ttggcgtttt agtggccttg atagctcaat tttactcagc aaaggcagca gtaggttttg
    21241 ctaaggaatt gacaaacgat ctttatcgtc atattctttc cttgcccaag gacagcagag
    21301 accgtctgac aacttctagt ttggtcactc gcttgacttc ggatacctac cagattcaga
    21361 ctggtatcaa tcaattcctg cgtctctttt tacgagcgcc cattatcgtt tttggtgcca
    21421 tttttatggc ttatcgaatc tcagctgagt tgactttctg gttcttagtc ttggttgcca
    21481 ttttgaccat tgtcattgta gggttatctc gattggtcaa tcctttctac agtagtctca
    21541 gaaagaaaac ggaccaactg gttcaggaaa cgcgccagca attgcaaggg atgcgggtta
    21601 ttcgtgcttt tggtcaagaa aaacgagagt tacagatttt tcaaaccctt aaccaagttt
    21661 atgctagatt acaagaaaag acaggtttct ggtctagttt attaacacct ctgacctatc
    21721 tgattgtcaa tggaactctt ctcgttatta tctggcaagg ctatatttca attcaaggag
    21781 gagtgctcag tcaaggtgct ctcattgctc ttatcaatta cctcttacag attttggtgg
    21841 aattggtcaa gctagccatg ttgatcaatt ccctcaacca gtcctatatc tcagtcaagc
    21901 gaatcgagga agtctttgtt gaggctccag aggatatcca ttcagagtta gaacaaaagc
    21961 aagctaccag agataaggtt ttacaagtcc aagaattgac ttttacctat cctgatgcgg
    22021 cccagccttc tctgagatac atttcctttg atatgactca aggacaaatt ctaggtatca
    22081 tcgggggaac tggttctggt aaatcaagct tggtgcaact cttacttgga ctttatccag
    22141 tagacaaggg gaacattgac ctttatcaaa atggacgtag tcctcttaat ttggagcagt
    22201 ggcggtcttg gattgcctat gtacctcaaa aggtcgaact ctttaaagga accattcgtt
    22261 ccaacttgac tctaggtttc aatcaagaag tatctgacca ggaactctgg caggccttgg
    22321 agattgcgca agctaaggat tttgtcagtg aaaaggaagg actcttggat gctctagttg
    22381 aggcaggggg gcgaaatttc tcaggtggac aaaaacaaag attgtctatc gcccgagcag
    22441 tcttgcgcca agctccgttt ctcatcctag atgatgcaac ctcggcactg gataccatta
    22501 cagagtccaa gctcttgaaa gctattagag aaaattttcc aaacacgagc ttaattttga
    22561 tctctcaacg aacctcaact ttacagatgg cggaccagat tctcctcttg gaaaaaggtg
    22621 agttgctagc tgttggcaag cacgatgact tgatgaaatc cagccaagtc tattgtgaaa
    22681 tcaatgcatc ccaacatgga aaggaggact agaatgaaac gacaaactgt aaaccagacg
    22741 ctcaaacgtt tagccgtaga tttagcaagc catcctttcc tccttttcct agcctttcta
    22801 ggaactattg ctcaagttgg cttatcaatt tacctaccta ttctgattgg gcaggtcatt
    22861 gaccaagtcc tagtggctgg ttcatcacca gttttttggc agatttttct ccagatgctc
    22921 ttggtggtaa taggaaatac tctggtacaa tgggccaatc ctctcctcta taatcgtcta
    22981 atcttctctt ataccagaga tttacgggag cgaatcatcc ataagctcca tcgtttaccg
    23041 attgcctttg tagataggca aggtagtgga gagatggtta gtcgtgtaac cacggacatc
    23101 gaacagttgg cagctggctt gaccatgatt tttaaccaat ttttcattgg tgttttgatg
    23161 attttggtca gtattctagc catgctccaa attcatctcc tcatgactct cttagtcttg
    23221 ctgttgacgc cactgtccat ggtgatttca cgctttattg ccaagaaatc ctatcatctc
    23281 ttccagaagc aaacagagac gaggggaatt cagactcagt tgattgaaga atcgcttagt
    23341 cagcagacta taatccagtc cttcaatgct caaacagaat ttatccaaag attgcgtgag
    23401 gctcatgaca actactcagg ctattctcag tcagccatct tttattcttc aacggtcaat
    23461 ccttcgactc gctttgtaaa tgcactcatt tatgcccttt tagctggagt aggagcttat
    23521 cgtatcatga tgggttcagc cttgaccgtc ggtcgtttag tgactttttt gaactatgtt
    23581 cagcaataca ccaagccctt taacgatatt tcttcagtgc tagctgagtt gcaaagtgct
    23641 ctggcttgcg tagagcgtat ctatggagtc ttagatagcc ctgaagtggc tgaaacaggt
    23701 aaggaagtct tgacgaccag tgaccaagtt aagggagcta tttcctttaa acatgtctct
    23761 tttggctacc atcctgaaaa aattttgatt aaggacttgt ctatcgatat tccagctggt
    23821 agtaaggtag ccatcgttgg tccgacaggt gctggaaaat caactcttat caatctcctt
    23881 atgcgttttt atcccattag ctcgggagat atcttgctgg atgggcaatc catttatgat
    23941 tatacacgag tatcattgag acagcagttt ggtatggtgc ttcaagaaac ctggctcaca
    24001 caagggacca ttcatgataa tattgccttt ggcaatcctg aagccagtcg agagcaagta
    24061 attgctgctg ccaaagcagc taatgcagac tttttcatcc aacagttgcc acagggatac
    24121 gataccaagt tggaaaatgc tggagaatct ctctctgtcg gccaagctca gctcttgacc
    24181 atagcccgag tctttctggc tattccaaag attcttatct tagacgaggc aacttcttcc
    24241 attgatacac ggacagaagt gctggtacag gatgcctttg caaaactcat gaagggccgc
    24301 acaagtttca tcattgctca ccgtttgtca accattcagg atgcggattt aattcttgtc
    24361 ttagtagatg gtgatattgt tgaatatggt aaccatcaag aactcatgga tagaaagggt
    24421 aagtattacc aaatgcaaaa agctgcggct tttagttctg aataagccat tctcttttga
    24481 aagtttatgg acgaaaaaag ttgccttcga gtgacttttt tgttacaata gctagaaaaa
    24541 ttgttcactg taatactcaa tgaaaatcaa agagcaaact aggaagctag ccgtaggttg
    24601 ctcaaagcac agctttgagg ttgtagataa gactgacgaa gtcagttcaa aacactgttt
    24661 tgaggttgca gatagaactg acgaagtcag ctcaaaacac tgttttgagg ttgcagatag
    24721 aactgacgaa gtcagtaacc atacctacgg caaggcgacg ttgacgcggt ttgaagagat
    24781 tttcgaagag tataaaggtg taccgagatg aacatctctt catcaatgtc tctggtcgtg
    24841 gagggaagga cctagctgcg attgcagact actagaagct aaaaaataat agatggaaaa
    24901 attacagttc tttctctcac agagagcttg tggttgctga aaacaagcag agaaagctgt
    24961 aaagttgggt tcatctgaaa cgagagaaga aaacatcatt ctcaagggag tgccctttat
    25021 cgcacagcct gttacaggag atatctgaga caggaatgag agataggaga aatcctataa
    25081 ttgaggtggc accgcgaatt tcgtcctcac gcaagttatt ttgcgtgggg atttttcata
    25141 cagtcgctac gaactagaat tcgaactgaa agggaaatta caatggaacg aatcattcat
    25201 ggagatgtct tatcaccaat cttggcttat atgcgcttaa aggggcaaca caaggttatc
    25261 ttagagagta ttccgagaga caaggaaaca gctcgttttt ctatcctagc ctataatcca
    25321 gtttttgaga ttaagtttga aaatggagtt ctctatcaaa atggtcaagt gattgatcgg
    25381 gatcccttgg atttccttta tgaagtgatt cataagagtc agcaccactc agagctccct
    25441 tttggtggtg gagccattgg ttttgtgggt tacgatatga tttcgcttta tgaagaaatt
    25501 ggtcaaatcc ctgaggatac aattgggacg ccagacatgc atttctttgt ctatgagagc
    25561 tatatggtct ttgaccacaa gaaggagaag atacatgtca tagaggatgc actttatagt
    25621 gagcgcagtc aagaggcctt ggaaaaatcc ttgaaccaag tgcttgagga gttacgcatt
    25681 cctgctccaa atgaatttga agacttggat ctatctccgt tagacttcaa accccatatc
    25741 gctcctcaga agtttgagga aatggtggaa accgctcgcg acttgattcg taacggagat
    25801 atgttccaat gtgtactcag tcagcgcttc tcagcagaag ttactggaaa tccatttgac
    25861 ttctacagaa atctccgcgt gattaatcct tctaattacc tttatttcta tgattttggg
    25921 gattatcaaa tcatcggagc tagtccagaa agtttggttt ctgtcaaaaa tggcatcgtg
    25981 acaaccaatc cgattgcagg gacgcgacca agaggggcta cggatgaaga agacaaggcc
    26041 ttggcgactg acctcctctc tgatgagaag gaaacagcag aacatcgaat gttggtagac
    26101 ttggggcgta acgatattgg ccgcatctct gaaacggcca gtgtccaagt tactaagtat
    26161 atggaggtgg agctcttccg ctacgtcatg catttgacca gtgtggtcaa gggacgtttg
    26221 cttgcagaac tcactgccat ggatgccttg aaagcaacgc ttcctgctgg aaccgtttct
    26281 ggagcaccaa aaattcgagc tatgagacgc atctatgaac tggaaacgga aaaacggagc
    26341 gtatatgcag gagcaatcgg ctacttgtct gcgacgggtg atatggattt ggccattgcc
    26401 atccgaacca tgattctcaa aaatcaaaga gcctatgtgc aggctggggc agggattgtc
    26461 tacgactcta tcgcccaaaa cgaataccaa gaaaccatta acaaagcaaa atctatgact
    26521 agaattggag aactaagacc atgattttat tgattgacaa ctatgattct tttacctata
    26581 acttggccca atacattggg aattttgcag aagttcaggt tctgagaaat gatgattcca
    26641 agctgtatga agaagctgaa aaagcagatg gtctggtctt ttctcctggt cctggttggc
    26701 cagttgatgc tggaaagatg gaagacatga ttcgtgattt tgcaggcaag aagccgattc
    26761 tagggatttg tttgggccac caagccattg cagaagtctt tggtggtaag ctaggtttgg
    26821 ctccaaaagt catgcatggg aaacagagca atatcaactt tgaagcgcca tctgttttgt
    26881 atcaaggtat tgaggatggc cgtgcggtca tgcgttatca cagtattttg attgaggaaa
    26941 tgccagaaga ctttgaagtg acagctcgtt cgactgatga ccaagccatc atggggattc
    27001 aacataaaaa cctaccgatt tatggcttcc agtaccatcc agagagcatt ggaacgccag
    27061 acggcttgtc ttctattcgg aattttatcg agaaggttgt aaagtgagga aactaggatg
    27121 aaagagatta ttgaaaaact agcaaaattt gaaaatttat caggtgtgga aatgacggat
    27181 gtcattgagc gtatcgtaac tgggcgtgta actgaagcgc agattgcttc tctcctctta
    27241 gctcttaaga tgaaggggga aacacctgaa gaacgcacag ccattgccca agtcatgaga
    27301 ggacatgccc agcatattcc aactgagatt catgatgcca tggacaactg tggtacaggt
    27361 ggggacaagt ctttcagttt taatatttcc acaactgcag cctttgtctt ggctggtggc
    27421 ggtattcaca tggccaagca tggtaaccgc tcgatttctt ctaaatctgg ttccgcagat
    27481 gtcctcgaag ccttgggaat caatcttgac ctcaaaccag ctgaactagg taaggtcttt
    27541 gataaaactg gaatcgtctt tctcttcgct aaaaatatgc acccagctat gaaatacatc
    27601 atgccagctc gtttggaatt gggaattcca acgattatga acttgactgg tccactgatt
    27661 catccaatgg ctttagaaac acagcttctt ggaattagtc gtccagaact cctagaaagt
    27721 acagctcagg ttttgaaaaa tatgggtcgc aaacgtgcca tcgtggttgc tggaccagaa
    27781 ggattggatg aagctggctt gaacggaaca accaagattg cacttcttga aaatggcgaa
    27841 atcagcttgt caagctttac tccagaggat ttgggaatgg aaggctatgc tatggaagat
    27901 attcgtggtg ggaatgctca ggaaaatgca gaaattttgc ttagcgttct gaaaaacgaa
    27961 gcaagtccat tcttggaaac gacagtcttg aatgctggtc ttggtttcta tgctaatggt
    28021 aagattgata gcatcaagga aggagttgcc ttggcccgtc aagtgattgc tagaggcaag
    28081 gcccttgaaa aactcagact gttacaggag taccaaaaat gagtcaggaa tttttagcac
    28141 gaatcttaga gcagaaggcg cgtgaggtgg agcagatgaa gctggagcaa atccagcctc
    28201 tgcgccagac ctatcgcttg gcagaatttt tgaagaatca tcaggaccgc ttgcaggtaa
    28261 tcgctgaggt caagaaagct agccctagtt tgggagatat caatctcgat gtggatattg
    28321 tgcaacaggc ccagacttat gaagaaaaca gagcagtgat gatttcggtg ttgacagatg
    28381 aagttttctt taaaggacat ttggattatc tacgggagat ttccagtcag gtagagattc
    28441 cgacgctcaa caaggacttt atcatcgatg aaaagcaaat catccgcgct cgcaatgcag
    28501 gtgcgacagt catcttgctc attgtggcag ccttggcaga agaacgtctt aaggaacttt
    28561 acgactacgc gacagtgctt ggtctggaag tcttggtgga aactcacaat ctagctgaac
    28621 tagaagtggc tcacagactt ggtgcccaga ttatcggggt caataaccgc aacttgacca
    28681 cctttgaggt cgacttgcag accagtgtag acttggctaa acatttcaaa gatgattgct
    28741 tgtacatttc tgaatctgct attttcacag ggcaggatgc ggaacgacta gccccatact
    28801 ttaacggaat tttagtaggg acagctctca tgcaggcaga ggatgtggcc cagagaatca
    28861 aggagttgca gattgacaaa ggttaagatt tgtggactat cgaccaaaga agcggtggaa
    28921 acagccgttt cagcaggagc cgactatatc ggttttgtct ttgcacctag taaaagacag
    28981 gtgactttag aagaggcagc tgtgttggca aagcttattc ctgcagatgt gaaaaaggtt
    29041 ggagtatttg tttcaccaag tcaggtagaa ctgctggaag cgattgacaa agttggcttg
    29101 gacttggttc aagttcacgg tcaggtggca gatgatttat ttgagaattt gccttgtgcc
    29161 agtattcagg ctgtgcaggt agatggaaat gggcatgtcc ccaattctca ggcagattat
    29221 ctactctttg atgcccctgt ggcaggaagt ggccagccct ttgattgggg tcaactggat
    29281 acgactggac tagcacagcc cttctttatc gcaggtggcc ttaatgaaga taatgtagta
    29341 aaagcaattc aacactttac tccctatgca gtagatgtat caagcggagt ggagacagat
    29401 ggacaaaaag atcatgaaaa gattagaaga tttatagaga gggtaaagaa tggcatatca
    29461 agaaccaaat aaagatggat tttacggaaa atttggcgga cgttttgtcc cagaaacatt
    29521 gatgacagca gttttggagt tggagaaggc ctaccgtgaa agtcaggcag acccaagttt
    29581 ccaagaggaa ttaaaccaac ttttgcgcca gtatgtggga cgtgaaactc ccctttacta
    29641 cgcaaaaaat ttgacccagc atatcggcgg agccaagatt tatctcaaac gggaagacct
    29701 taaccataca ggagcccaca agattaacaa tgccttagga caagtttggc ttgccaaacg
    29761 catgggtaaa aagaaaatta tcgcagaaac gggtgctggt cagcacggtg tggcaactgc
    29821 aactgctgcg gccctcttta acatggaatg taccatctac atgggtgagg aagatgtcaa
    29881 acgccaagcc ctcaatgtct tccgcatgga gcttttggga gccaaggtcg aggctgtaac
    29941 agatggttcg cgcgtgctca aggatgcggt caatgcagcc cttcgttcat gggtggctaa
    30001 tatcgacgat acccactata tccttggttc tgccttgggg cctcatccat ttccagaaat
    30061 cgttcgtgac ttccaaagtg tcatcggtcg agaggctaaa caacagtacc gtgacttgac
    30121 aggtcgagat ctgccagatg ccctagtagc ctgtgttggt ggtgggtcga atgctatcgg
    30181 gctcttccat ccctttgtag aagatgagtc agtagctatg tatggaactg aagcagcagg
    30241 gcttggtgtg gatacagagc accacgcagc taccttgacc aagggtcgtc caggtgtcct
    30301 tcacggttcc ctcatggatg tgctccaaga tgcccatggt caaattcttg aagccttctc
    30361 tatctcagca ggtttggact atcctggtat cggtccagag cattctcact accacgatat
    30421 taaacgtgcc agctatgtcc ctgtgacaga cgaagaagcc ttggaaggat tccaactctt
    30481 gtctcgtgtg gaagggatta tcccagcctt ggaatctagc catgctatcg cctttgcggt
    30541 gaaattggcc aaagaacttg gaccagaaaa gtctatgatt gtctgcctat caggtcgtgg
    30601 ggacaaggat gtggttcaag tcaaggaccg cttggaagca gatgcagcaa agaagggaga
    30661 agctcatgcc taagacacta acagaaaaat taaatgctat taaagcagct ggaaaaggaa
    30721 tttttgttcc ctatatcatg gctggtgacc acgagaaagg tttggatgga ctaggagaaa
    30781 caatccactt tttagaagat ttgggtgtct ctgcaattga agtgggtatt cccttttcag
    30841 accctgttgc agatggacct gttatcgaag aagctggctt gcgcagttta gcccacggga
    30901 cctctaccca ggctttagtt gaaaccttga aaaccattga aacagagatt ccactggtca
    30961 tcatgaccta cttcaacccc ctctttcagt acggtgtgga gaactttgtc aaagatctgg
    31021 ctgatacagc tgtgaagggc ttgattatcc cagacctgcc tcatgaacat gccaactttg
    31081 tagaaccatt tttggcaaat acagatatcg ccttgattcc tttagtaagc ttgaccacag
    31141 gaattgagcg ccagaaagag ttgattgaag gggcggaggg attcatctat gccgttgcca
    31201 tcaatggagt gacagggaaa tcaggcaatt accgtgcaga tttggacaag cacttggcgc
    31261 aacttcatca agtggccgac atcccagtct tgacaggttt tggtgtatct agtcaagccg
    31321 atctggaacg cttcaatgcg gtgtcagatg gtgttatcgt cggttcgaaa atagtaaaag
    31381 ctctccacca aggagagccg attcaggact ttatcaaaca agcagtagct taccaaaaat
    31441 aatcacacaa gcagcaagta agaggaaagg cacgaaagga agtctttcct ttttcttttg
    31501 caggagaaag gccaggatac ccgtcgcaga agcgaactga atcaagatca gtaactccgt
    31561 tacgctaaag acgagagcgc aagaagctaa aaaaaggaaa tcccctgcac ccatgcggat
    31621 atcgataaaa tgagccaaaa ttccaagtat gaggaaggag accatgacca gattccagcc
    31681 agaggaagct attaggatta ggtggaaagt catccagacc agtaagggat attcctgatg
    31741 gcgaaagtcg tagatgccca aggtcaaacc agcagtgatt aggatgactt tactcaagga
    31801 aagtaattcc caagagtaaa gcagaaagag gagtcctaag cctagttcaa agagggcata
    31861 ccagacagga tagcggacct tgcagtagcg acagcaaaag cgattgaaga cctgtgagag
    31921 aatcggaatc aaatctaagg gacgcaagcg agtctgacag gaatcgcagt gactggctga
    31981 actgataatg gattgctctg gaaaacggtc aatgaccaaa ccaagaaagg aagctagaat
    32041 gctcccgacg agaaaaaaat aaaaatcaat catacttatc tattcgtaaa aaatgggaga
    32101 aatagtataa tggagaaaca tagataaggt ggtgaggaaa agatgacaat tcgttttgaa
    32161 gaaaaggtga gcacagaaaa cgctcaattt gtatgccagt ggtccaactc cctaggcaaa
    32221 tcctttcaag aacaatggat gggaccaagg attccttttc tactgaccct tcaagccttg
    32281 gaaggagtct tttcaatctt tgatgaacaa gagtttgtgg gatttatcca gaaaatcagg
    32341 ctagaagaca gcaatcttca tatcgggaga ttttttatca acccccagaa acaggagcaa
    32401 gacttaggta gccaggcttt aaggaaattt gttagtttgg cctttgaaaa tgaagatata
    32461 gatagtattt ctctaaatgt cttcgaggca aatcaaagag ttcagaatct ttaccaaaaa
    32521 gaaggatttg aaatcgttca aatggttgaa gcacctgtac gaaa
//
LOCUS       DAVMEI010000008        29841 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVMEI010000008 DAVMEI010000000
VERSION     DAVMEI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29841
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.008"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..210)
                     /locus_tag="VYQ24_000309"
     CDS             complement(<1..210)
                     /locus_tag="VYQ24_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1967496.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMP"
     gene            444..1202
                     /locus_tag="VYQ24_000310"
     CDS             444..1202
                     /locus_tag="VYQ24_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1967497.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALIKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            1211..2014
                     /locus_tag="VYQ24_000311"
     CDS             1211..2014
                     /locus_tag="VYQ24_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1967498.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            2036..3295
                     /gene="rseP"
                     /locus_tag="VYQ24_000312"
     CDS             2036..3295
                     /gene="rseP"
                     /locus_tag="VYQ24_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1967499.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRHKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            3308..5161
                     /locus_tag="VYQ24_000313"
     CDS             3308..5161
                     /locus_tag="VYQ24_000313"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1967500.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            5261..6640
                     /gene="bglA"
                     /locus_tag="VYQ24_000314"
     CDS             5261..6640
                     /gene="bglA"
                     /locus_tag="VYQ24_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1967501.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            6832..8640
                     /gene="glmS"
                     /locus_tag="VYQ24_000315"
     CDS             6832..8640
                     /gene="glmS"
                     /locus_tag="VYQ24_000315"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1967502.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            8759..9808
                     /locus_tag="VYQ24_000316"
     CDS             8759..9808
                     /locus_tag="VYQ24_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1967503.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(9908..13705)
                     /gene="spuA"
                     /locus_tag="VYQ24_000317"
     CDS             complement(9908..13705)
                     /gene="spuA"
                     /locus_tag="VYQ24_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1967504.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQEASV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            14122..14535
                     /gene="rpsL"
                     /locus_tag="VYQ24_000318"
     CDS             14122..14535
                     /gene="rpsL"
                     /locus_tag="VYQ24_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1967505.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            14555..15025
                     /gene="rpsG"
                     /locus_tag="VYQ24_000319"
     CDS             14555..15025
                     /gene="rpsG"
                     /locus_tag="VYQ24_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1967506.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            15450..17531
                     /gene="fusA"
                     /locus_tag="VYQ24_000320"
     CDS             15450..17531
                     /gene="fusA"
                     /locus_tag="VYQ24_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1967507.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            17635..22026
                     /locus_tag="VYQ24_000321"
     CDS             17635..22026
                     /locus_tag="VYQ24_000321"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1967508.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            22128..22391
                     /locus_tag="VYQ24_000322"
     CDS             22128..22391
                     /locus_tag="VYQ24_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1967509.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            22384..22662
                     /locus_tag="VYQ24_000323"
     CDS             22384..22662
                     /locus_tag="VYQ24_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1967510.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            22683..22835
                     /locus_tag="VYQ24_000324"
     CDS             22683..22835
                     /locus_tag="VYQ24_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967511.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            22857..24098
                     /locus_tag="VYQ24_000325"
     CDS             22857..24098
                     /locus_tag="VYQ24_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1967512.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            24108..24338
                     /locus_tag="VYQ24_000326"
     CDS             24108..24338
                     /locus_tag="VYQ24_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1967513.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            24606..25328
                     /locus_tag="VYQ24_000327"
     CDS             24606..25328
                     /locus_tag="VYQ24_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1967514.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            25545..26879
                     /gene="pepC"
                     /locus_tag="VYQ24_000328"
     CDS             25545..26879
                     /gene="pepC"
                     /locus_tag="VYQ24_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1967515.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(26935..27846)
                     /locus_tag="VYQ24_000329"
     CDS             complement(26935..27846)
                     /locus_tag="VYQ24_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1967516.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(27870..28673)
                     /locus_tag="VYQ24_000330"
     CDS             complement(27870..28673)
                     /locus_tag="VYQ24_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1967517.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(28701..29690)
                     /gene="manX"
                     /locus_tag="VYQ24_000331"
     CDS             complement(28701..29690)
                     /gene="manX"
                     /locus_tag="VYQ24_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1967518.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNIKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         8488 a         5776 c         6966 g         8611 t
ORIGIN      
        1 aggcatacca gttgtttcga ggtaagggat cttagacggt ttttgaaagt catatttctt
       61 cattagactt ccacaatcag ggcaagatgg agcctcataa tccagcttag cgataatttc
      121 tttgtgggta tccatattga tgatatctag aatcttgatg tttgggtctt taatatcgag
      181 cagttttgtg ataaaatgta attgttccat atgaatcttt ctaatgagtt gttttgtcgc
      241 ttttcattat aggtcatatg ggactttttt tctacaataa aataggctcc ataatatcca
      301 tagtggattt acccactaca aatattatag agccattttt ttgaaaaatg ggcttggtgc
      361 ctgagaataa atagcttagt gatagaagaa aatggggaaa tatggtataa tgaaacgata
      421 gatttttgaa taggaataag atcatgtttg gattttttaa gaaagataag gctgtggaag
      481 tagaggttcc gacacaggtt cctgctcata tcggcatcat catggatggc aatggccgtt
      541 gggctaaaaa acgtatgcaa ccgcgagttt ttggacataa ggcgggcatg gaagcattgc
      601 aaaccgtgac caaggcagcc aacaaactgg gcgtcaaggt tattacggtc tatgcttttt
      661 ctacggaaaa ctggacccgt ccagatcagg aagtcaagtt tatcatgaac ttgccagtag
      721 agttttatga taattatgtc ccggaactac atgcgaataa tgttaagatt caaatgattg
      781 gggagacaga ccgcctgcct aagcaaacct ttgaagcttt aatcaaggct gaggaattga
      841 ctaagaacaa cacaggattg attcttaatt ttgctcttaa ctatggtgga cgtgctgaga
      901 ttacacaggc gcttaagttg atttcccagg atgttttaga tgccaaaatc aacccaggtg
      961 acatcacaga ggaattgatt ggcaactatc tctttaccca gcatttgcct aaggacttac
     1021 gagacccaga cttgattatc cgtactagtg gagaattgcg tttgagcaat ttccttccat
     1081 ggcagggagc ctatagtgag ctttatttta cggacacctt atggcctgat tttgacgaag
     1141 cagccttgca ggaagctatt cttgcctata atcgtcgcca tcgccgattt ggaggagttt
     1201 aggaggaaat atgacccagg atttacagaa aagaaccttg tttgcaggga ttgccctggc
     1261 tattttccta ccaattttaa tgattggggg cctcttgctt cagatagcaa tcggaatcat
     1321 agccatgcta gccatgcatg aacttttgaa gataagaggt ctagcgacca tgacgatgga
     1381 gggcctcttg actctctttg caacctttgc attaaccatt cccttggaga attacctgac
     1441 ttttttgcca gttgatggga atgtggttgc ctatagtgtt ttgatttcaa tcatgttagg
     1501 aacgaccgtt tttagcaagt cttatacgat tgaggatgcg gttttccctc ttgctatgag
     1561 cttctacgtg ggctttggat ttaatgcttt actagatgct cgtgttgcag gtttggacaa
     1621 ggctctctta gccttgtgta tcgtctgggc gacagacagt ggtgcctatc ttgttgggat
     1681 gaactatggg aaacgaaagt tagcaccaag ggtatcgcct aataaaaccc ttgagggtgc
     1741 cttgggtggt attttaggag caattttagt aaccattatc tttatgatag ttgacagtac
     1801 agttgctctt ccatatggaa tttacaagat gtcagtcttt gctattttct ttagcattgc
     1861 tggacaattt ggtgatttac tagaaagttc gatcaaacgt cattttggtg ttaaggattc
     1921 tgggaaattt atccctggac atggtggtgt tttggatcgt ttcgatagta tgttgcttgt
     1981 atttccaatc atgcacttat ttggactctt ttaatcaaaa gacggaggaa acgctatgct
     2041 cggaatttta acctttattc tggttttcgg gattattgta gtggtgcacg agttcgggca
     2101 cttctacttt gccaagaaat cagggatttt agtacgtgaa tttgccatcg gtatgggacc
     2161 taaaattttt gctcacattg gcaaggatgg aacggcctat accattcgaa tcttgcctct
     2221 gggtggctat gtccgcatgg ccggttgggg tgatgataca actgaaatca agacaggaac
     2281 gcctgttagt ttgacacttg ctgatgatgg taaggttaaa cgcatcaatc tctcaggtaa
     2341 aaaattggat caaacagccc tccctatgca ggtgacccag tttgattttg aagacaagct
     2401 ctttatcaaa ggattggttc tggaagaaga aaaaacattt gcagtggatc acgatgcaac
     2461 ggttgtggaa gcagatggta ctgaggttcg gattgcacct ttagatgttc aatatcaaaa
     2521 tgcgactatc tggggcaaac tgattaccaa ttttgcaggt cctatgaaca attttatctt
     2581 aggtgtcgtt gttttttggg ttttaatctt tatgcagggt ggtgtcagag atgttgatac
     2641 caatcagttc catatcatgc cccaaggtgc cttggccaag gtaggagtac cagaaacggc
     2701 acaaattacc aagattggct cacatgaggt tagcaactgg gaaagcttga tccaagctgt
     2761 ggaaacagaa accaaagata agacggcacc gactttggat gtgactattt ctgaaaaggg
     2821 gagtgacaaa caagtcactg ttacacccga agatagtcaa ggtcgttacc ttctaggtgt
     2881 tcaaccgggg gttaagtcag attttctatc catgtttgta ggtggtttta caactgctgc
     2941 tgactcagct ctccgaattc tctcagctct gaaaaatctg attttccaac cggatttgaa
     3001 caagttgggt ggacctgttg ctatctttaa ggcaagtagt gatgctgcta aaaatggaat
     3061 tgagaatatc ttgtacttct tggcaatgat ttccatcaat attgggattt ttaatcttat
     3121 tccgattcca gccttggatg gtggtaagat tgtgctcaat atcctagaag ccatccgcca
     3181 caaaccattg aaacaagaaa ttgaaaccta tgtcaccttg gccggagtgg tcatcatggt
     3241 tgtcttgatg attgctgtga cttggaatga cattatgcga ctctttttta gataatcgag
     3301 gaatattatg aaacaaagta aaatgcctat cccaacgctt cgcgaaatgc caagcgatgc
     3361 tcaagttatc agccatgctc ttatgttgcg tgctggttat gttcgccaag tttcagcagg
     3421 tgtttattct tatctaccac ttgccaaccg tgtgattgaa aaagctaaaa acatcatgcg
     3481 ccaagaattc gaaaagattg gtgctgttga gatgttggct ccaacccttc ttagtgcaga
     3541 attgtggcgt gaatcaggtc gttacgaaac ctatggtgaa gacctttaca aactaaaaaa
     3601 ccgtgaaaaa tcagacttta tcttaggtcc aactcacgaa gaaaccttta cagctattgt
     3661 ccgtgattct gttaaatctt acaagcaatt gccactcaac ctttatcaaa ttcagcccaa
     3721 gtatcgtgat gaaaaacgcc cacgtaatgg acttcttcgt acacgtgagt ttatcatgaa
     3781 ggatgcttat agtttccacg ctaactatga tagtttggat agtgtttatg atgagtacaa
     3841 agcagcctat gagcgtattt tcactcgtag tggtttagac ttcaaggcta ttattggtga
     3901 cggtggagcc atgggtggta aggatagcca agaatttatg gccattacat ctgctcgtac
     3961 agaccttgac cgctgggttg tcttggacaa gtcagttgcc tcatttgacg aaattcctgc
     4021 agaagtgcaa gaagaaatca aggcagaatt gctcaaatgg atagtctctg gtgaagatac
     4081 cattgcttac tcaagtgagt ctagctatgc agctaactta gaaatggcaa caaacgagta
     4141 caaaccaagc aaccgtgttg tcgctgaaga agaagttact cgtgttgcaa cgccagatgt
     4201 taaatcaatt gatgaagttg cagccttcct caatgttcca gaagaacaaa cgattaaaac
     4261 cctcttctac attgcagatg gtgagcttgt tgcagccctt ctagttggaa atgaccaact
     4321 caacgaagtc aagttgaaaa atcacttggg agcagatttc tttgacgttg ctagcgaaga
     4381 agaagtggcg aatgttgttc aagcaggatt tggttcactt ggaccagttg gtttgccaga
     4441 gaatattaaa attattgcag atcgtaaggt gcaagatgtt cgcaatgcag ttgtcggtgc
     4501 taacgaagat ggctaccact tgactggtgt gaacccaggc cgtgatttta ctgcagaata
     4561 tgtggatatc cgtgaagttc gtgagggtga aatttcccca gatggacaag gtgtccttaa
     4621 ctttgcgcgt ggtattgaga tcggtcatat tttcaaactc ggaactcgct actcagcaag
     4681 catgggagca gatgtcttgg atgaaaatgg tcgtgctgtg ccaatcatca tgggatgtta
     4741 cggtatcggt gtcagccgtc ttctttcagc agtgatggag caacacgctc gcctctttgt
     4801 taacaaaacg ccaaaaggtg aataccgtta cgcttgggga atcaatttcc ctaaagaatt
     4861 ggcaccattt gatgtgcatt tgattactgt caatgtcaag gatgaagaag cgcaagcctt
     4921 gacagaaaaa cttgaagcaa gcttgatggg agctggttat gaagtcttga cagatgaccg
     4981 taacgaacgt gtcggtgtta aatttagcga tagtgacttg attggtcttc caatccgtat
     5041 cactgttggt aaaaaagcgg ccgatggcat tgtagaagtt aagatcaagg caactggtga
     5101 caccattgag gttcatgcag ataacgtgct tgaaacgctt gaaatcctaa gcaagaaata
     5161 aaaactataa tcagaagaaa aacaaggaaa aaatgtaact agtttttacc ttgttttttc
     5221 tgtataatgg gagaaatgag tagataaaga ggtaaatgtt atgctaagat ttccaaagga
     5281 ttttgtctgg ggatcctcta cttctggacc gcagacagaa ggacgtgtag ctggtgacgg
     5341 taagggagac aatctctggg attactggtt ccaagtggag ccaaatcgtt actataatgg
     5401 gattggttca gataagacat cgacttttta tgaaaattgg gagcgggata ttgagctttt
     5461 gttagagact ggtcacacag tctttcggac ttctattcag tggtcacgga tttttccaca
     5521 aggctgtgga aaagtcaacc ctcaaggtgt ggatttttat cgtaaggtct ttgaggctat
     5581 taaggctaaa ggaattcgtc tgttagtcaa tctctatcat tttgatttac cttttgccct
     5641 tcaagaggat ggtgatggtt gggaaaataa ggcgacagtc tcagcctatg aagactatgc
     5701 tcgtttttgt tttgagactt atggagattt agtggatcaa tggattacct ttaacgagcc
     5761 catcgttcct gtagaatttg gttattttta tgatgcccat tatccacata aggtggatgc
     5821 agaggcagct gttaaggtag cctatcatac acaattggcc agcagccggg ctgttaaggc
     5881 ttgccatgaa cttttgcctg attccaagat tgggattgtc ctcaacttga caccggctta
     5941 tccacgtagc cagcatcctg ctgatgtcaa ggcagctcgt attgcggccc tttttcaggc
     6001 ccaatctttc ttagatccat ctgtcttggg gacttatcca caggagttgg tagaaatctt
     6061 gcatgaacac ggtcttttac ctgatgctac agaggaagag ttggaactca ttcgtgataa
     6121 tacggtggac ttccttggtg tgaactacta tcaacctttg cgtgttatgg ctcctcgatt
     6181 tgctaagcat ccagatagtc cactcttacc agaacatttt tacgagcctt atgtgatgcc
     6241 tggacgtaaa atcaattctc accgtggttg ggagatttat gagcaaggga tttatgacat
     6301 cgcccaaaat atcaaggaaa attatggcaa tattgagtgg atgttgactg agaatggtat
     6361 gggtgttgaa ggggaagaaa aattccgtca agatggaatg attcaagatg attaccgtat
     6421 tgactttgta aaaggtcatc ttcgtgaact tcaccgtgcc attgaagatg gtgccaactg
     6481 taagggctac ttgatttgga cctttattga ctgctggtca tggctcaaca gctataaaaa
     6541 tcgctatggt ttggtcgaat tagacttgga aacgcaagaa cgtcgtctga agaaatcagg
     6601 ccactggttc aaagaattaa gcgataataa tggattttaa aaaggactct gactgattat
     6661 cccaatcggt cagagttttt tgcttacagg agcttaattt gaactatacc aatttttact
     6721 cttgacaagt gaaagaaaca agtatatact gtttttgctg attctataaa agatgaatta
     6781 gactatacca attttaagga gaaagcacag cttgcctgtg tcgtatatac tatgtgtgga
     6841 attgttggtg ttgttggaaa cacaaatgca actgatattt tgattcaagg gcttgaaaag
     6901 cttgaatacc gtggctatga ttctgcggga atttttgtcc tagatggtgc tgataaccat
     6961 ttggtgaagg ctgttggtcg tatcgcagaa ttgtctgcca agacagctgg tgttgaggga
     7021 acaactggta tcggacatac tcgttgggca actcatggga aaccaacgga agacaatgct
     7081 cacccacacc gctctgagac agaacgtttt gtcttggttc acaatggggt gattgaaaac
     7141 taccttgaaa ttaaagaaga ataccttgct gggcaccact tcaaagggca aacagatacg
     7201 gaaatcgccg tacatttgat tggaaaattt gcggaagaag acggtctctc agttcttgaa
     7261 gcctttaaaa aagctcttca tattatccgt ggttcatatg cctttgcctt gattgactct
     7321 gaaaatccag atgtcatcta tgtagcgaaa aacaaatctc cacttttgat tggtcttggg
     7381 gaaggctaca atatggtctg ctcagatgct atggctatga ttcgtgaaac caaccaatac
     7441 atggaaattc atgaccaaga gttggtaatc gtcaaggctg atagcgtgga agttcaagac
     7501 tatgatggta acagtcgtga acgtgctagc tatactgcgg aacttgactt gtcagatatc
     7561 ggtaagggaa cttatcctta ctacatgctt aaggaaattg atgagcaacc aactgttatg
     7621 cgtaaactca ttcaagccta cacggatgat gctggtcaag tagtgattga tcctgctatc
     7681 attaaggctg ttcaagacgc agaccgcatc tacatccttg cagctggaac atcttaccat
     7741 gcaggatttg cttctaagaa aatgttggaa gaattgacag atacaccagt tgaacttggc
     7801 atctcatctg agtggggcta cggtatgcca cttctcagca agaaaccact cttcatcttt
     7861 atcagccaat ctggtgaaac agcggatagt cgtcaagttt tggtcaaggc taatgaaatg
     7921 ggaattccaa gcttaacagt gacaaacgtt ccaggttcaa ccctctcacg tgaagccaac
     7981 tataccatgc tccttcacgc aggtcctgaa attgccgtgg catcaactaa agcctataca
     8041 gcgcaaatcg cagcccttgc cttccttgca aaagcagtcg gagaagcaaa tggtaatgct
     8101 aaagcgcaag cctttgacct ggttcatgaa ttgtcaatcg tagctcagtc tatcgaatca
     8161 actctttcag agaaagaaac cattgaagcc aaggttcgtg aacttcttga aacaactcgt
     8221 aacgcctttt acatcggacg tggtcaagat tactacgtag ccatggaagc aagtctcaaa
     8281 ctcaaagaga tttcttatat ccagtgtgaa ggttttgcgg caggagaact caagcacgga
     8341 accattgcct tgattgaaga aggaacacct gtcttggctc tcttgtcaga tccagtcctt
     8401 gccaaccaca ctcgtggaaa tatccaagag gtggcagccc gtggtgctaa ggtcctcact
     8461 atcgcagaag aaaatgttgc taaagatacc gacgatatcg tccttacgac tgtacaccct
     8521 tacctctcac caatttcaat ggtcgtaccg acgcaattgg ttgcttactt tgcaaccctc
     8581 caccgtggcc tcgatgtgga taaaccacgt aaccttgcta aatcagtaac ggtagaataa
     8641 gctaaaaaag tctagttatc taggcttttt ctaatgagca aattggttgc ttgtactcaa
     8701 gattcgtgtt agaataattt ttcaaattga aggacagaga tagacaagga gaatcacaat
     8761 ggtagaattg ggaatttcaa catttgggga aataacggag cttgaaggga ctggacaaac
     8821 ttacagtcat gctgaacgca ttcgccagtt ggtggcagag attgagctgg ctgacaaggt
     8881 tggtttggac gtatatggga ttggcgagca ccatcgggcg gattttgcag tatcagcccc
     8941 agagattgtc ctggccgctg gggcagtcaa taccaagaaa atccgtttga ccagtgcagt
     9001 cagcattctg tcaagcatgg atccgattcg tttgttccaa cagtatgcca ctatcgatgc
     9061 tttgtcaaat ggacgatcgg agattatggc tggaaggggc tctttcacgg aatctttccc
     9121 tctgtttggt tatgacttga aagactacga ctctcttttt gatgagaaat tagacttgct
     9181 tcagttagtc aatgaaaaga ccaagttaga ctggcaaggt cgattgactc aaacgattgc
     9241 tggtaaagaa gtttatcctc gtccagttca ggacaaattg cccttgtgga tagctacagg
     9301 tggtcatgtc gaatcaacag tgaagattgc tcaggctggt ctaccgattg tatatgctat
     9361 tattggtggt aatccacgtt attttaaaaa gttgattcag gcttatcgtg agattggaag
     9421 cgaagcgggc catgctgaca aagacttgaa agtgggagcc cattcttggg ggtggattgc
     9481 ggaagatggt gagcaggcgg tgaaagatta tttccatccg actaagcaag tggtggatgc
     9541 tatttccaaa gaccgtccgc actggcagga attgcgttat gagcaatatt tggagcaggt
     9601 agggccaaat ggcgccatgt ttgttggcaa tccagatcag gtggccgaaa aattgattcg
     9661 catgatagag gatttaggat tggaccgctt catgcttcat ctaccgcttg gttctatgcc
     9721 tcatgaccag gttctgagag ctattgaact cttcggaaca caagttgcac ccaaagtacg
     9781 agcttatttt gctatgaaag aggcttaata aaaaatccta atcaagttta ttaggacaac
     9841 aaatattgta caaaaattaa tcccccgagc tataatgctt gggggatttt tctataggag
     9901 ggctagttta gttctctttt ttatttttta gtaagaaacc gagaccaagg agcgcaagga
     9961 ggctgattcc tgcaaatagg agtttgtttt cgtttttgat tcctgtattt ggaagttcag
    10021 cttgtttatc tggggttgca ggtatatttt ccttgctaga gttttctacc gattcgtttc
    10081 gaaccgcttc ttttacagat gcttcttgtg cttcttgtgc tggttgttca gcttgtgaat
    10141 cagctgtagc ttggctaggt ttattttccg catcagctac tttggcatct ggtttagtag
    10201 aatcaggtct agcttctgga gctgggtctt gatgtgcagg gtgagaagct tcattttgat
    10261 gttcaggctt ggaaggggtt gagtctggtt tctcttctgc agttgactca tggctagttc
    10321 cattttgaga gactcgaaga acagtagctg taagggcatt caatttcaag cctttttcag
    10381 tccattcaag tcctttcggg ttggcaattc cgactgatcc tgcttggttt tcatctgcca
    10441 aaacttccgc atttcttaga tgtgcaaagg cagttcccaa attaaattcg cgagcttttt
    10501 catccgcatt gacaaagact gcgtagatat caccgtttgg agcagtgatt tggtagccaa
    10561 tcactacatc ctctttttcc acaccatttt ggcctgggac agtgatgagg tggacacggt
    10621 ctttgatatc ttgaagactc ttaagtcgga aggcatctgt agattgacga agggcaatca
    10681 aacctttcat atagtcacgg ctcttgacat tttcaggata agctttacca tctgtagcct
    10741 tagtccagtc aaacttgttg actgcgtcgc tagaatcgta agaatcatgg atgaagtaag
    10801 gatagtcaaa tgggttgccg tccttatcac gcaacaagtg agatttgttt ggaaccttat
    10861 cctctgctac tggagtcttg taggctgggt cacggaattg tttagtacgt ccatattcct
    10921 gaccggagtg gataaatgga gttccttgag ctgtcaagac catgagattt ccaagtcgta
    10981 aacgacggtg gatttcagca tagttctcag ccttgcttgg gtcttttttg atagactggg
    11041 caatgatgtc aaagagggtc aagttatcat gggctgcgat gtattggatg acatctccag
    11101 ggctgtcagc ttcaaagtta gttggttgag caatgagatt tttaaagatg gtgttgacat
    11161 cacgcttgcc acctgtgata aaggcaggtt gaccttcgtt tggataacca gatttgaggt
    11221 tgttacggat gtcatctgaa aagacagcga cagtatcggt atgtttcatc caatcttggt
    11281 cagcagcctt agtaggcatg ttttcatcac cggcataggt tctccaacct tcaccaagca
    11341 tgatgaggtt tggattgagg gcgcgtgcag ccttgtaagc ttcttcgata gaagcggcgt
    11401 catggtctcc catcatatcg aagcggaagc catccacttt gtaggtatca actaggtatt
    11461 tgatagagtc aactaggagc cgtttggtca tatggtgggt tgtccccaag cgtccaccac
    11521 caaagctagt tcgaggtgtg ccatcggcat ccataaagtg gtagtagttt ggctctaggt
    11581 cttcaaagat atcgactttg gctgtgtggt tataaacgac atctaggata gctcccatac
    11641 cacgtttgtg gatttcgttg atgaggtttt taaattctgc gattcgtttt tctggattct
    11701 taggatcgct tgagtacata ccagtcaagg agaagtagtt ttgagggtca tatccccagt
    11761 tgtagttgct gttgcttgaa gcgtagtcag acaagcgttc atggtttttc aattcattga
    11821 caaagtagta agacaagact ggaaggagct ggatatgggt tacacccaag tctttgagat
    11881 agtctagttt ttcaatgaag gcttcaaaag tcccaaatgg tttggtcaag tcttttgcaa
    11941 tggcaggatc tgaagtgaag tcacgcacat gagcttcgta gataacggcg tcttcacgag
    12001 tcttgaaatt gtgaatctta ccataagtca agtcttgagg tccgagttta gctggatcta
    12061 caaaggcggc tttagccact ttatgggcat cgtcaatttt ggcatcgtcg ctattccaag
    12121 cagcaagaga tttagcgtaa ggatcgagtg caagaacagt tttaccttga cgctcgattt
    12181 ggtattgata atagtagcca gtgaaatctg tgattccgag tttgtttgtg ctgtctagag
    12241 tttgtttcca agttcctctt tccccttttt cgagagcgac agttccaact actttgtcag
    12301 ggtcattctt gtcgtagaca acaacagaaa ccttatcagc acttggtgac caaagggtca
    12361 aatcaacttg ttttccttct tcttttaggt cagctcccag tttgccatca tagctgtatg
    12421 tctcatcttt caggcgccag cttgttttgg tagtgaattg gtcggaattg tagctaacag
    12481 tataaggatg ttttgtgtca gagaaatctc cgctgtaggt cactttctta ccagcttcat
    12541 cgattgcaac atcggtaata gttaccttgt ttcctaggtg attagtgatg ttggagtgtt
    12601 tgaggatatc ttctttttta gcaccgacaa gtgttgaaaa gctactttca atgctagaag
    12661 tgcctacgtg ttgggctcct gtcatacgga tatcatggac atagtatgga tttgtgtaaa
    12721 tcgattcatc atcgtctttt aggaaaattt ggctatgatt tttcaaatct gtgaacttat
    12781 aattttcttt acggattttc acgtcgtctc cttgtttgct ctcatctagt aataaaaatc
    12841 caaattctct tgcggcttca ttaagaggaa tgtcgatata gcggccatat ttgcctgtag
    12901 ccgtaaagtc tgttccgtca ggccattgag cgctacttgg attttttaca tctccccagt
    12961 accagagaga tttcttgtca tagttgccat ctgtgcggta gtagttgacg cgaacagttc
    13021 ctgcaggctg tggctcgtaa gagaaaacct tgtaatcttg gtctaaccaa gcctcgttca
    13081 tttttggaac tagtttttct acagatttat cgccggttag attttttcca gctgtattgt
    13141 tgatgaggaa gctaattttc ttggcttgtt ctccctttaa tttgacatct aggtaatagc
    13201 cgtagtcatc tttcttggca tccttgaagg acaaagctcc gtttggccag ttttcagatg
    13261 gtttttcaac atcgtcccaa gtccatagtc cttgagcatc cttgttttct tcaggaagtt
    13321 ttttgacatg gatacggaag tagttatctt cgatgggctc ttctgcctta gtttcagttg
    13381 ttactggact agtagaagta gttggctctg ttgcaggttt ttcttctgtt ttctctaggg
    13441 aagcgtttgc atttctaggt gaagaaatat cactttcgtt cttaccagag ccagctgctg
    13501 tatcagtaag aggttgggca agggtggttg tatttctgtt gttgggttcg ttagtggttg
    13561 gtgtactttc gtcggatgag atagttggtg tagccatggc aagcaagaca aggcttgcgc
    13621 cgataaggac agaaccagtt ccatttttga gggaacggat gctgtagacc atttttttct
    13681 cagtgtgaga tggtgttttt ctcataatga ttctccaatc aataaatttc tatatcagta
    13741 tagcatgtag taaaacaata tgcaagcgtt ttctttgatt tgtgaaaaaa attcttactt
    13801 ttgagaagtt ttctgtacta ttttatatat cagtataaat gacggagata aaaatgtcac
    13861 ataagccaag ccgtattatt aggaagatga tgtgttccca gctattttta ttcagaaatt
    13921 gatctctaac attaccaata aaaaagaaaa atatcttgac aagcaaggga aaattttgtt
    13981 acaataatag acggtacttt ttacttttgg tctctcaaga gtgtacaggg acgtgctgac
    14041 aaatgttgca aaagtacaca cagatgatat ctgtcaccaa gtgtatcatc accaaaaata
    14101 aaaaaacaca ggagaatgta gatgcctaca attaaccaat tggttcgcaa accgcgtaaa
    14161 tcaaaagtag aaaaatctaa atcaccagct ttgaacgttg gttacaatag tcataaaaaa
    14221 gttcaaacaa acgtttcttc accacaaaaa cgtggtgttg caactcgtgt tggaacaatg
    14281 acacctaaaa aacctaactc agcccttcgt aaattcgctc gtgtacgttt gagcaacctt
    14341 atcgaagtta ctgcctacat cccaggtatc ggacacaact tgcaagagca cagcgtggtg
    14401 cttcttcgcg gtggacgtgt aaaagacctt ccaggggtac gttaccatat cgtccgtggt
    14461 gcacttgata ctgcaggtgt taacgatcgt aaacaaggcc gttctaaata cggtactaaa
    14521 cgtccaaaag cataaggaaa ggggataaag agaaatgagt cgtaaaaata gagctccaaa
    14581 acgtgacgta ttgccagatc cgctatacaa ttcacaacta gttactcgtc ttatcaaccg
    14641 cgttatgctt gatggtaaac gtggtactgc tgcttcaatc gtttacggtg cttttgagca
    14701 aatcaaagaa gctactggca acgatgcact tgaagtattt gaaacagcta tggaaaacat
    14761 catgcctgta cttgaagtac gtgcacgtcg tgttggtggt tctaactacc aagtcccagt
    14821 tgaagttcgt ccagaacgtc gtacaacact tggacttcgt tggttggtaa caatcgctcg
    14881 tcttcgtggt gaacacacaa tgcaagaccg tcttgcaaaa gaaatcttgg atgctgctaa
    14941 caacactggt gcagcagtta agaaacgtga agatactcac cgtatggctg aagctaaccg
    15001 tgcattcgca cacttccgtt ggtaagatag gatgcgaaag cgttaagaaa gtcccagaga
    15061 aaatagggaa tcgaagcagg ttgcggttgc aaccaatgag attcatcttt ttctccagac
    15121 ttttagcttg agctcaacta aatcatgatg ctaggcacgg taaggatgca aggtgaaaat
    15181 aggaaactga cgcagtattc aacgaataca aggagtttta tctttttcac gcagcatccc
    15241 gttccagctc acatcggcta actaacttta gcctgggttc aaattagcta aatcgattag
    15301 tattagctat aactcagctt accatctcgt aagttgaaac caacaatagc atgaaaacat
    15361 tgagaacggg taggtcctgc ctatccgttt ttattaaaat cgtgttataa tagaatagaa
    15421 atcaaaaata aataggagaa acaaacctca tggcacgcga attttcactt gaaaaaactc
    15481 gtaatatcgg tatcatggct cacgtcgatg ccggtaaaac aacaactact gagcgtattc
    15541 tttactacac tggtaaaatc cacaaaatcg gtgaaactca cgaaggtgcg tcacaaatgg
    15601 actggatgga gcaagagcaa gaacgtggta tcacgatcac atctgctgcg acaacagctc
    15661 aatggaacaa ccaccgcgta aacatcatcg acacaccagg acacgtggac ttcacaatcg
    15721 aagtacaacg ttctcttcgt gtattggatg gtgcggttac cgttcttgac tcacaatcag
    15781 gtgttgaacc tcaaactgaa acagtttggc gtcaagcaac tgagtacgga gttccacgta
    15841 tcgtatttgc caacaaaatg gacaaaatcg gtgctgactt cctttactct gtaagcacac
    15901 ttcacgatcg tcttcaagca aatgcacacc caatccaatt gccaatcggt tctgaagatg
    15961 acttccgtgg tatcattgac ttgatcaaga tgaaagctga aatctatact aacgaccttg
    16021 gtacggatat ccttgaagaa gacatcccag ctgaatacct tgaccaagct caagaatacc
    16081 gtgaaaaatt gattgaagca gttgctgaaa ctgacgaaga attgatgatg aaatacctcg
    16141 aaggtgaaga aatcactaac gaagaattga aagctggtat ccgtaaagcg actatcaacg
    16201 ttgaattctt cccagtattg tgtggttcag ccttcaaaaa caaaggtgtt caattgatgc
    16261 ttgatgcggt tatcgactac cttccaagtc cacttgacat cccagcaatc aaaggtatta
    16321 acccagatac agacgctgaa gaaactcgtc cagcatctga cgaagagcca tttgcagctc
    16381 ttgccttcaa gatcatgact gacccattcg taggtcgttt gacattcttc cgtgtttact
    16441 caggtgttct tcaatcaggt tcatacgtat tgaatacttc taaaggtaaa cgtgaacgta
    16501 tcggacgtat ccttcaaatg cacgctaaca gccgtcaaga aatcgacact gtttactcag
    16561 gtgatatcgc tgctgccgtt ggtttgaaag atactacaac tggtgactca ttgacagatg
    16621 aaaaagctaa aatcatcctt gagtcaatca acgttccaga accagttatc caattgatgg
    16681 ttgagccaaa atctaaagct gaccaagaca agatgggtat cgcccttcaa aaattggctg
    16741 aagaagatcc aacattccgc gttgaaacaa acgttgaaac tggtgaaaca gttatctcag
    16801 gtatgggtga acttcacctt gacgtccttg ttgatcgtat gcgtcgtgag ttcaaagttg
    16861 aagcgaacgt aggtgctcct caagtatctt accgtgaaac attccgcgct tctactcaag
    16921 cacgtggatt cttcaaacgt cagtctggtg gtaaaggtca attcggtgat gtgtggattg
    16981 aatttactcc aaacgaagaa ggtaaaggat tcgaattcga aaacgcaatc gtcggtggtg
    17041 tggttcctcg tgaatttatc ccagcggttg aaaaaggttt ggtagaatct atggctaacg
    17101 gtgttcttgc aggttaccca atggttgacg ttaaagctaa gctttatgat ggttcatatc
    17161 acgatgtcga ctcatctgaa actgccttca agattgcggc ttcactttcc cttaaagaag
    17221 ctgctaaatc agcacaacca gctatccttg aaccaatgat gcttgtaaca atcactgttc
    17281 cagaagaaaa ccttggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgtagatg
    17341 gtatggaagc acacggtaac agccaaatcg ttcgtgctta cgttccactt gctgaaatgt
    17401 tcggttacgc aacagttctt cgttctgcat ctcaaggacg tggtacattc atgatggtat
    17461 ttgaccacta cgaagatgta cctaagtcag tacaagaaga aattattaag aaaaataaag
    17521 gtgaagacta atccgtcctc actctagaag gaagtcactt agtggcttcc ttttgtcttt
    17581 agaaaatacc tctaaatatg gtaaaatagt agaagaataa tgtgaggaaa atgaatgtca
    17641 aatagttttg aaattttgat gaatcaattg gggatgcctg ctgaaatgag acaggctcct
    17701 gctttagcac aggccaatat tgagcgagtt gtggttcata aaattagtaa ggtatgggag
    17761 tttcatttcg tattttctaa tattttaccg attgaaatct ttttagaatt aaagaaaggt
    17821 ttgagcgaag aattttctaa gacaggcaat aaagctgttt ttgaaattaa ggctcggtct
    17881 caagaatttt caaatcagct cttgcagtcc tactataggg aggctttctc tgaaggtcca
    17941 tgtgctagtc aaggttttaa gtccctttat caaaatttgc aagttcgtgc tgagggtaat
    18001 cagctattta ttgaaggatc tgaagcgatt gataaggaac attttaagaa gaatcatctt
    18061 cctaatttag ccaaacaact tgaaaagttt ggttttccaa cttttaactg tcaagttgag
    18121 aagaatgatg tcctgaccca agagcaggaa gaggcctttc atgctgaaaa tgagcagatt
    18181 gttcaagctg ccaatgagga agcgctccgt gctatggaac aactggagca gatggcacct
    18241 cctccagcgg aagagaaacc agtctttgat tttcaagcga aaaaagctgc agctaaaccc
    18301 aagctggata aggcggagat tactcctatg atcgaagtga cgacagagga aaatcgtctg
    18361 gtatttgaag gggttgtttt tgatgtggag caaaaagtga ctagaacagg tcgtgtttta
    18421 atcaacttta aaatgacgga ctatacttca agtttttcta tgcaaaagtg ggttaaaaac
    18481 gaggaagagg cccagaagtt tgacctcatc aagaagaatt cttggctccg agttcgaggg
    18541 aatgtggaga tgaataactt cacacgcgat ttgactatga acgtacagga tctgcaggaa
    18601 gttgttcact atgagcggaa ggatttgatg ccagaaggtg agcgtcgggt tgagtttcat
    18661 gctcatacta acatgtcgac tatggatgct ttgccagagg tcgaagagat tgttgcaaca
    18721 gctgctaagt ggggacacaa ggcggttgct atcacagacc atgggaatgt ccagtccttt
    18781 ccacatggct ataaggcggc taagaaagcg ggaatccagc tgatctatgg gatagaagcc
    18841 aatatcgtgg aggaccgtgt ccctatcgtc tataacgaag tggagatgga cttatcagaa
    18901 gcaacctacg tggtctttga cgtggaaacg acgggacttt cagctatcta taatgacttg
    18961 attcaggttg cggcctctaa gatgtacaag gggaatgtta ttgctgaatt tgatgaattt
    19021 atcaatcctg ggcatccctt gtcagctttt actacagagt taactggaat tacagatgat
    19081 catgtcaaaa atgccaaacc actagaacaa gttttgcaag aattccaaga attttgcaag
    19141 gatacggtcc tagttgccca caatgctgcc tttgacgttg gctttatgaa tgctaattat
    19201 gagcgtcatg gtcttccaaa gattagtcag ccagttattg atacgctgga gtttgctaga
    19261 aacctctatc ctgagtataa acgccatggt ttggggcctt tgaccaagcg ttttggtgtg
    19321 gccttggaac atcaccacat ggccaactac gatgcggaag cgactggtcg tctgcttttc
    19381 atctttatca aagaggtagc agaaaaacat ggtgtgaccg atttagctag actcaacatt
    19441 gatctaatca gtccagattc ttacaaaaaa gctcggatca agcatgcgac catctatgtc
    19501 aagaatcagg taggtctaaa aaatatcttt aagctggttt ccttgtctaa taccaagtat
    19561 tttgaaggag tgccacggat tccgagaacg gttctagatg cccatcgaga gggcttgatt
    19621 ttaggttcag cctgttcaga gggtgaagtt tttgacgtgg tcgtttctca aggtgtggat
    19681 gcggcggttg aggtggccaa gtattatgac tttatcgagg tcatgccacc ggctatctat
    19741 gcgcccttga ttgctaaaga gcaggtcaag gatatggagg aactccagac cattatcaag
    19801 agtttgatag aggttggaga ccgccttggc aagcctgttt tggctacggg aaatgttcac
    19861 tatatcgaac cggaagaaga gatttatcgt gaaattatcg tccgtagttt gggacagggt
    19921 gcgatgatta accgaactat cggtcatggt gaacatgccc aaccagcacc acttccaaag
    19981 gctcattttc gaacgactaa tgagatgttg gatgaatttg cctttttggg agaggaactg
    20041 gctcgtaaac tggttattga aaacaccaat gccttggcag aaatatttga acccgttgaa
    20101 gtcgttaagg gtgacttgta tacgcctttc atcgacaagg ctgaagaaac agttgctgag
    20161 ttgacctata agaaagcttt tgagatttat ggaaatccgc tgccagatat tgttgatttg
    20221 cggattgaaa aagaattaac atccatactg gggaatggat ttgctgtgat ttatctggca
    20281 tcgcagatgc tggtgcaacg ttctaatgaa cggggttatt tggttggttc tcgtgggtct
    20341 gtcggatcta gtttcgttgc gaccatgatt ggaattacgg aggtcaatcc tctctctcct
    20401 cactatgtct gtggtcagtg tcagtacagt gagtttatca cagatggttc gtacggttca
    20461 ggatttgata tgccccataa ggactgtcca aactgtggtc acaaactcag taaaaacgga
    20521 caggatattc cgtttgagac cttccttggt tttgatgggg ataaggttcc tgatattgac
    20581 ttgaacttct cgggagaaga tcagcctagc gcccacttgg atgtgcgtga tatctttggt
    20641 gaagaatatg ccttccgtgc gggaacagtt ggtacggtag ctgccaagac tgcctatgga
    20701 tttgtcaagg gttacgagcg agattatggc aagttttatc gtgatgcaga agtagaacgc
    20761 ctcgctcaag gagcggcggg tgtcaagcgg acaacaggcc aacacccggg gggaatcgtt
    20821 gttattccga actacatgga tgtctacgat tttacgcctg tccagtatcc agcagatgat
    20881 gtcacggctg aatggcagac cactcacttt aacttccacg atatcgatga gaacgtcctc
    20941 aaactcgatg tactgggaca tgatgatccg accatgattc gaaaacttca ggatttgtct
    21001 ggtattgacc ctaataaaat tcctatggat gacgaaggcg tgatggcact cttttctggg
    21061 actgatgtgc taggggtaac acctgaacaa attggaacgc ctacgggtat gttggggatt
    21121 ccagagtttg gaacaaattt cgtacgtgga atggtagacg aaacccatcc gacaaccttt
    21181 gcggaattgc ttcagctgtc tggtctgtcc cacggtactg atgtttggtt ggggaatgct
    21241 caggatctga ttaagcaagg aatagcggac ctatcgactg ttatcggttg tcgggacgac
    21301 atcatggttt acctcatgca tgcgggtctg gaacctaaga tggcctttac cattatggaa
    21361 cgggtacgta agggtttgtg gctaaagatt tcagaagagg agagaaatgg ctatatcgaa
    21421 gcaatgaagg ctaataaggt gccagagtgg tatatcgaat cctgtgggaa aattaagtac
    21481 atgttcccta aggcccatgc ggcagcctac gttatgatgg ccttgcgtgt agcttacttc
    21541 aaggttcacc atcctattta ttactactgt gcttacttct ccattcgtgc taaggctttt
    21601 gatatcaaga ccatgggtgc gggcttggag gtcatcaagc gcagaatgga agaaatctct
    21661 gaaaaacgga agaacaatga agcctctaat gtggaaatcg atctctatac aactcttgag
    21721 attgtcaatg agatgtggga acgaggtttc aagtttggta aattagatct ctaccgtagt
    21781 caggcgacag agttcctcat cgacggggat acccttatcc caccatttgt agcaatggat
    21841 ggtctgggag agaacgttgc caagcaactg gtgcgggcgc gtgaagaggg agaattcctc
    21901 tctaaaacag aactacgcaa gcgtggtgga ctctcatcaa ccttggttga aaagatggat
    21961 gagatgggta ttcttggaaa tatgccagag gataaccagt tgagtttgtt tgatgagttg
    22021 ttttaaaaaa ttgcttaata atctattaaa agaggctaac gtatatccaa tagatttaca
    22081 ttagctttct tttttgttaa aatagtctat ggaaagaggg tgagagtatg tcaaagatga
    22141 gtataagcat ccgtctggat agtgaggtta aggagcaggc ccaacaggtg tttagtaatc
    22201 tgggaatgga tatgacaaca gctattaata ttttccttcg tcaggcaatt caatatcagg
    22261 gattaccttt tgatgttaga ctagacgaaa atcggaagtt gctccaagcg ttaacggatt
    22321 tagaccaaaa tcgtaatatg agccagtctt ttgaatcagt ctcagatttg atggaggact
    22381 tacgtgctta agattcgtta tcataaacag tttaaaaaag attttaagtt ggctatgaag
    22441 cgtggtttga aggcagaatt attagaagaa gttttgaatt ttctggttca agaaaaagaa
    22501 catcctgcca gatatcgtga tcattcattg acggcatcca agcattttca aggagttcgt
    22561 gaatgccata cccagccaga ttggcttttg gtttataaag tagacaagtc ggaattgatt
    22621 ttaaatttgc tgaggacagg cagtcacagt gatttatttt aatctatttt aagggggttc
    22681 tcatgaaact aagaatattt gcggaagata agccggctaa gaaggtattt gaatatcaat
    22741 tagaacttgc tgatcgtaca attcttctat cgacagcact cttgtcaggt gctattgctt
    22801 tagcaggaat cttttctgct ttgaaagaaa aataaaaata gaaaagagaa aacagaatgg
    22861 ttttaccaaa ttttaaagaa aatctagaaa aatatgcgaa attgttggtt gcgaacggaa
    22921 ttaacgtgca acctggtcac actttggctc tctctattga tgtggagcaa cgtgaattgg
    22981 cacatctaat cgtgaaagaa gcttatgcct tgggtgcgca tgaggtcatc gttcagtgga
    23041 cagatgatgt gattaaccgt gagaaattcc tccatgcccc gatggagcgt ttggacaatg
    23101 tgccagaata caagattgct gagatgaact atcttttgga gaacaaggct agccgtcttg
    23161 gggttcgttc atctgatcca ggtgccttga acggagtgga cgctgacaag ctttcagctt
    23221 ctgctaaagc tatgggactt gccatgaagc ctatgcgtat cgcaactcaa tctaacaagg
    23281 ttagctggac tgtagcagcc gctgctggac ttgagtgggc taagaaagtc ttcccaaatg
    23341 ctgcgagcga cgaagaagca gttgatttcc tttgggatca aatcttcaaa acttgtcgtg
    23401 tctacgaagc agatcctgtt aaggcttggg aggaacatgc agccattctc aagagcaagg
    23461 ccgatatgct taataaggag caattttcag cccttcacta cacagcgcca ggaacagatt
    23521 taacacttgg tttgccaaag aaccacgttt gggaatcagc tggtgctgtc aatgcacagg
    23581 gcgaagaatt cttgccaaat atgccgacag aagaggtctt cacagcgcct gacttccgtc
    23641 gtgcagatgg ttatgtcact tctacaaaac cgcttagcta caacggaaat atcattgaag
    23701 gcattaaggt gacctttaag gatggacaaa tcgtagatat cactgctgag aagggtgatc
    23761 aggttatgaa agaccttgtc tttgaaaatg cgggtgcgcg tgccttgggt gaatgtgcct
    23821 tggtaccaga tccaagtcca atttctcagt caggcattac cttttttaac acccttttcg
    23881 atgaaaatgc gtcaaaccac ttggctatcg gtgcagccta tgcgactagc gttgttgatg
    23941 gagcggagat gagcgaagag gagcttgaag ctgcagggct taaccgttca gatgttcacg
    24001 tagactttat gattggttct aaccaaatgg atatcgatgg tattcgtgag gatggaacgc
    24061 gggtacctct tttccgtaat gggaattggg caaattaagg agataatatg ttaggaagta
    24121 tgttcgttgg tctcctagtg ggatttttag caggtgctat gaccaatcgt ggagagcgaa
    24181 tgggatgttt tggaaaaatg tttctcggtt ggatcggagc ctttctaggt cacttgctct
    24241 ttggaacttg ggggccagtt ttatcaggaa cagctattat cccagcgatt ttaggagcca
    24301 tgattgtttt agctattttt tggagacgag gaagttaatt tcctaaatct gatactcaat
    24361 gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc tcaaagcact gctttgaggt
    24421 tgcagataga actgacgaag tcagctaaaa acactgtttt gaggttgcag atagaactga
    24481 cgaagtcagt aacatatata cggcaaggcg acgttgacgc ggtttgaaga gatttttgaa
    24541 gagtatgaaa cgaaaaggag gttggtcatt gtactagcct ccttttgata tgatataata
    24601 gttctatgag attagataaa tttttagttg cctgtgcggt gggaagtcgg actgaggtca
    24661 aaaacttgct caaggctggg cgcgtgactg taaatggtaa aaaagaaaaa tcagctaaat
    24721 tgcagattga tgaaaaaata gatgagattc gctttgatgg gcaagtgttg gagtatgaag
    24781 agtttgtcta ctacatgatg aacaagccca aaggagttat ctcagcgact gaggatccca
    24841 agcacagaac cgttctggac ttgttggatg acttggcgcg gagtaaggaa gttttcccag
    24901 taggacgctt ggatattgac acgcatggtc ttttgctctt gaccaatgat ggtcagctgg
    24961 ctcatgttct tctttcgccc aagcgtcatg tggacaagac ttatctggca caagtcaagg
    25021 gaatcatgac ccaagaagat gtggagacat ttgctgaggg tattcctctc aaagacttta
    25081 cctgtcaacc cgctatactg gagcttgtat ccatagatac agaaaagaat caaagccaaa
    25141 tccgtgtgac cattgcagaa gggaagtttc atcagatcaa gcgtatggtg ggctactgtg
    25201 gcaaggaagt ggtggacttg caacgattga ctatgggaac tttagtatta gatgagaacc
    25261 tggaacgagg ggaatggcgt cgcttgacca aggaagaatt agaaattctc cgcgctaata
    25321 ttatttaatt tagtttaata ctcaatgaaa atcaaagagc aaactaggaa actagccgca
    25381 ggctgtactt gagtacggca aggcgacgtt gacgtggttt gaagagattt tcgaagagta
    25441 taaattcgaa aaaggtgagg gacaatgtcc tcgcctttta tgttttttag ttgcttcctt
    25501 tgtgaaaaga gttataatag actgtagaat aaaaggagga atctatgaac gcgattcaag
    25561 aatcatttac tgataaacta tttgccaact atgaagcaaa tgtaaaatac caagcgattg
    25621 aaaatgctgc cagccacaac ggaatttttg cagctctgga acgtcgccaa agccatgtag
    25681 acaacacacc tgttttctca ttggatttaa ccaaggacaa ggtcactaac cagaaagcgt
    25741 ctggtcgttg ctggatgttt gcggctctca acaccttccg ccacaaactc atctcgcaat
    25801 acaaattgga gaactttgag ttgtcacaag cccacacttt cttctgggat aagtatgaga
    25861 aatcaaactg gttcttggag caagtcattg cgacttcaga ccaagaattg actagccgca
    25921 aggttagctt cttactccaa acacctcaac aagatggcgg tcaatgggat atggtcgttt
    25981 ccctctttga aaaatacggt gtcgtgccta agtcagttta tcctgagtct gtttcatcta
    26041 gcagcagtcg tgagctaaat gcgatcctta ataaattgct tcgtcaagat gctcaaatct
    26101 tgcgtgactt gcttgtttct ggtgcagatc aagcgactgt tcaagctaag aaagaagacc
    26161 tcttgcaaga aatctttaac tttcttgcta tgtcattagg acttccacca cgcaagtttg
    26221 actttgctta tcgcgataaa gataacaact acaaaagtga aaaaggaatc acaccacaag
    26281 agttttacaa gaaatatgtc aatcttcctt tagaagacta cgtttctgtt atcaatgctc
    26341 caactgctga taaaccttac ggaaaatctt acacagttga gatgttgggg aatgtggttg
    26401 gtagccgtgc agttcgttac atcaacgttc caatggagcg cttgaaagaa ttggcgattg
    26461 cccaaatgca agcaggtgag actgtttggt ttggttctga tgtcggccag ctcagtaacc
    26521 gtaaggctgg catccttgcg acagatgttt atgactttga atcaagcatg gacattaaac
    26581 ttactcaaga caaggctgga cgtttggact atagcgaaag cttgatgacc cacgccatgg
    26641 tcttgacagg tgttgacttg gacgaaaatg gtaaatcaac caagtggaag gttgaaaact
    26701 catggggaga caaggtcggt acagatggtt actttgttgc ctcagacgct tggatggacg
    26761 aatacacata ccaaatcgtt gttcgtaagg aattgctgac agcagaagaa caagctgcct
    26821 atggagcaga accaattgta cttgcaccat gggatccaat gggagccttg gctgaataaa
    26881 agcatagaaa aaaggaatca gattttagaa cctggttcct ttttagttgc ttgattacat
    26941 gatgtgaaga acatgtgcca caatacccac tgcgaagagg gcaaggataa tagtgattgg
    27001 agatactttt ttcttaagta agtacatgca aagtaaagta aggagtagtc ctgataatcc
    27061 aggaatcaac atatccaagt tttgttggaa agtagtaact ttttcaggag tttgagacaa
    27121 tccttgtcct acttgtgcga atgcttcttg gataccttta gacccttctg gcaatttatc
    27181 ccaatggata taagcctttt catctagttg aactttagaa acatcgaaag caaatttaat
    27241 atttacccag cgttgaacaa ggacagcaag aatgaacatc ccaaggatag aagctccttt
    27301 agtgatatct tgaaggatac caccagacat atctttagtg atttctgatc cagccttgta
    27361 tccaatctct tgaacatacc acaagaatga catacgaatc aagttccatg caacaaagaa
    27421 gaggagtgga cccaagatat tgccagtaag ggcaagtgaa gcaccgagag atccaaggat
    27481 tgggcgtact gtaaaccaga atactgggtc accgatacca gcaagaggtc ccatcatacc
    27541 gattttgacc ccttggatag cagcgtcatc gatttccaca ccgttagcac gttcttcttc
    27601 aagcgcaaga gtaaccccca tgactggagc agctacgtat ggatgagtgt tgaagaactc
    27661 aaggtgacgc tcaagagcag cgatttgatc ttctttttta gtatagagtt ttttgatagc
    27721 tggaatgagt gtataagccc agcccaagtt ttgcatccgt tcaaagttcc aagacccttg
    27781 taagaaggtt gaacgccacc aaactttttt acgatctgat ttagttaatt gaagtttttc
    27841 agtcatgatg ttttcagtcc tttcttatct tagtagtctt ctaggatatc gccgattggg
    27901 tcgttagaag ttgcggctcc tccgccacca tttccaccag ttttagaaag gtgaaggtag
    27961 ataagagcga tagcaacacc gatagcaccg aatccgatta gagtaatatc tgacacagca
    28021 gcgagaacga aaccaagagc gaagaatggc catacttcac gagttgccat catgttgata
    28081 accatggcgt aaccaacggc aacgaccata ccaccaccga tagccatacc atctttgagc
    28141 cagtctggca tggcactaag gatactttgt acagtttcag ttggtaccat aaggagaaga
    28201 gctgcaggaa gcgcgatacg aagtccttgg aagagtagcg caatgaaatg cgcacgctcc
    28261 acagcgccga agtcaccttt tttagcggca gcatctgcag tatgaaccaa accaactgaa
    28321 attgtacgaa caatcattgt caagaaaagt ccagctacgg caagagggat agcaaccgct
    28381 tgggcaacac cgataccagt cttggtaaag tcaccaccaa gaaccatgat aatggcagca
    28441 gcgacagaag caagtgcagc atcaggagcg atagcagcac cgatatttga ccaaccaagg
    28501 gcaatcattt gaagcgatcc accgaggata atccctgctt ccaagtgacc tgttacaagc
    28561 ccaataaggg tacaggctac aagtggttgg tgaaattgga actggtcgag gatgccttca
    28621 agacctgcaa agaaggctac aacgactact aaaaccatag aaataataga catgtttaaa
    28681 atcctttcat aaataatggc ttatttgaca ttggctttgt taatcaagtc aaacaaatct
    28741 tttttagaat catttggtac tttacggaca tcaaattcaa cacccaagtc acgcattttt
    28801 tcaaatgtcg caacatcttc tttgtccata gacaaaacgg tattgaccaa tgttttacct
    28861 gttgagtgag ccatagaacc aacgttaaga gtcttgattg gcacgccgcc ttcgatggca
    28921 cgaagggcat cttgaggtgt ttcaaacaag ataagggcat gtgtttctcc aaaacgtggg
    28981 tcttttgaaa tctcaatcag tttttgaatt ggaaccacgt tagccttgat attacctgga
    29041 gctgcttgtt taatcaattc tttacgaagg tcgtctttag ccacgttatc tgaagcaacg
    29101 atgatacgat ttgcttttga atctggagtc caagcagttg caacctgacc gtgaagtaga
    29161 cgtgtgtcaa gacgggcaag attgattttc aatttaccgt ctccgataac agttccttct
    29221 gggatagcag cttgggcaac tggagcagct gcagcgcttg caacttcttc aactggattt
    29281 agctcttctg gaagagcttt gatgccatcc ttggcttctt taatgatatt agcagcgact
    29341 ttttctacac ctgcagcagc gtccatgagg cgctctgtgt aggcttgaat caacatcggt
    29401 aagttaagtc ctgtgatgat ggcaaactta cgctcaggat tttctcccat cacgcgacta
    29461 gcttggttaa atggagaacc actccaaagg tcagccaaaa ctagaacctc atcttctgcg
    29521 tcaaatgcag caacagcgtt attaaactta gcgtatagat catcaggacc ttcatttggc
    29581 ataaaggtca caacttgaac cttttcttgt tcaccaaaga tcatagatcc tgactgatga
    29641 atacccgcag caaattcgcc gtggctcgca ataatgattc cgatactcat tattgtcatt
    29701 cctccttttt tgttttagtt ttcatttaag aaaactttaa gactgtttaa gtataaaccg
    29761 ttttcataaa aaaagcaact atttatcata aaaaattaaa aaatttttat ttttatatgt
    29821 tgatatgttg agtttttaag g
//
LOCUS       DAVMEI010000009        28527 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVMEI010000009 DAVMEI010000000
VERSION     DAVMEI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.009"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            15..293
                     /locus_tag="VYQ24_000332"
     CDS             15..293
                     /locus_tag="VYQ24_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967519.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            295..2358
                     /locus_tag="VYQ24_000333"
     CDS             295..2358
                     /locus_tag="VYQ24_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1967520.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(2436..3692)
                     /locus_tag="VYQ24_000334"
     CDS             complement(2436..3692)
                     /locus_tag="VYQ24_000334"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1967521.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            3834..6299
                     /locus_tag="VYQ24_000335"
     CDS             3834..6299
                     /locus_tag="VYQ24_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1967522.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            6364..7275
                     /locus_tag="VYQ24_000336"
     CDS             6364..7275
                     /locus_tag="VYQ24_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1967523.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            7505..8203
                     /gene="dapD"
                     /locus_tag="VYQ24_000337"
     CDS             7505..8203
                     /gene="dapD"
                     /locus_tag="VYQ24_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1967524.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            8271..9401
                     /locus_tag="VYQ24_000338"
     CDS             8271..9401
                     /locus_tag="VYQ24_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1967525.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            9413..9952
                     /locus_tag="VYQ24_000339"
     CDS             9413..9952
                     /locus_tag="VYQ24_000339"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1967526.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            9936..10613
                     /locus_tag="VYQ24_000340"
     CDS             9936..10613
                     /locus_tag="VYQ24_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1967527.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(10680..11579)
                     /gene="galU"
                     /locus_tag="VYQ24_000341"
     CDS             complement(10680..11579)
                     /gene="galU"
                     /locus_tag="VYQ24_000341"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1967528.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(11601..12617)
                     /locus_tag="VYQ24_000342"
     CDS             complement(11601..12617)
                     /locus_tag="VYQ24_000342"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1967529.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            12829..13210
                     /locus_tag="VYQ24_000343"
                     /pseudo
     CDS             12829..13210
                     /locus_tag="VYQ24_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(13384..13809)
                     /locus_tag="VYQ24_000344"
     CDS             complement(13384..13809)
                     /locus_tag="VYQ24_000344"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1967530.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(13877..14527)
                     /gene="phoU"
                     /locus_tag="VYQ24_000345"
     CDS             complement(13877..14527)
                     /gene="phoU"
                     /locus_tag="VYQ24_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1967531.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(14542..15294)
                     /gene="pstB"
                     /locus_tag="VYQ24_000346"
     CDS             complement(14542..15294)
                     /gene="pstB"
                     /locus_tag="VYQ24_000346"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1967532.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(15296..16111)
                     /gene="pstA"
                     /locus_tag="VYQ24_000347"
     CDS             complement(15296..16111)
                     /gene="pstA"
                     /locus_tag="VYQ24_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1967533.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(16104..16967)
                     /gene="pstC"
                     /locus_tag="VYQ24_000348"
     CDS             complement(16104..16967)
                     /gene="pstC"
                     /locus_tag="VYQ24_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1967534.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(17085..17960)
                     /locus_tag="VYQ24_000349"
     CDS             complement(17085..17960)
                     /locus_tag="VYQ24_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1967535.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(18127..19458)
                     /locus_tag="VYQ24_000350"
     CDS             complement(18127..19458)
                     /locus_tag="VYQ24_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1967536.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(19459..20166)
                     /locus_tag="VYQ24_000351"
     CDS             complement(19459..20166)
                     /locus_tag="VYQ24_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1967537.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(20250..20552)
                     /locus_tag="VYQ24_000352"
     CDS             complement(20250..20552)
                     /locus_tag="VYQ24_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1967538.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(20539..20760)
                     /locus_tag="VYQ24_000353"
     CDS             complement(20539..20760)
                     /locus_tag="VYQ24_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1967539.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            20722..21281
                     /locus_tag="VYQ24_000354"
                     /pseudo
     CDS             20722..21281
                     /locus_tag="VYQ24_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21596..23287)
                     /locus_tag="VYQ24_000355"
     CDS             complement(21596..23287)
                     /locus_tag="VYQ24_000355"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1967540.1"
                     /translation="MNTKELIASKLSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTIAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            23423..23869
                     /gene="argR"
                     /locus_tag="VYQ24_000356"
     CDS             23423..23869
                     /gene="argR"
                     /locus_tag="VYQ24_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1967541.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            23920..26454
                     /gene="mutS"
                     /locus_tag="VYQ24_000357"
     CDS             23920..26454
                     /gene="mutS"
                     /locus_tag="VYQ24_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1967542.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            26601..28295
                     /gene="patA"
                     /locus_tag="VYQ24_000358"
     CDS             26601..28295
                     /gene="patA"
                     /locus_tag="VYQ24_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1967543.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLAG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         8164 a         5511 c         6311 g         8541 t
ORIGIN      
        1 aggaggaaat aagtatgtta aaagaagtat taaccgttgc aaaagttgcg aaaaaatctt
       61 cattattttt gggtggtgtc gcatttggta cccttggttt gaaaatctta gcaagtaagg
      121 aagctaaaaa aggttattct aaagctttgg ctaaggctta caagttgaaa gacgagctag
      181 atgcatctgt ttctgttgtg aagcaacatg gagacgatgt cttgcaagat gccaaatatt
      241 tgtacgagca agagaaaaaa gaagagcaat tagatagcct tataggtgaa taatatgtct
      301 tttaaagtgc tacatagagg ataccaacat atccgactat catcttcttt ttcacttacc
      361 ttggatattc aagactatct tcgttccttg gcgagagatg aaaaggggat tgagtctatc
      421 cagttttaca tggatcaaca gcactttact ctacgcataa aagaaggctt ttctgtatta
      481 gataatgcag aagccttttt aaaaagaatt gataaaggga aagtttctga gttgatgact
      541 cttcccattc gtagagaaga gagtgcttat tctattgttt caggtgcagc ggttaagcgt
      601 gtactttttc gtagttttgt gccgtatcct attcgctata tatggacttg ttatcaggct
      661 ttgggttata ttagagaagc ctatcaaaca ctagcgcgta aggaactaac tatggaagtc
      721 ttggactgtt cggctatttt attgtctttg tttatgaacc aatccaagac agctagcaat
      781 atcatgttta tgcttgattt ggggaatcat ttagatcagt ggtccttgaa aaaaactgca
      841 acagatttag aacagagtct tcttgcaaaa gagagcgatg tattcctagt acagggcgat
      901 acggttgtta gtataaagag ttccgatgtt caaataggag atgtcttgat cttatctcaa
      961 ggaaatgaaa ttctgtttga tgggcaagta gtttcaggtt taggtatggt caacgaaagt
     1021 tccttgacag gagagagttt tccagttgaa aaaagagagt ctgatttggt ttgtgcaaat
     1081 acagtattag aaactggaga gttacgcatt cgtgtaacag ataatcagat gaacagccgt
     1141 attttacagc tgattgagtt gatgaagaaa tctgaagaaa acaagaaaac gaaacaacgc
     1201 tatttcatca agatggcgga taaagtcgtc aaatataatt tcttgggggc tgggctgact
     1261 tacctattga caggttcttt ttctaaggct atttctttcc tattggtcga tttctcctgc
     1321 gctttgaaaa tctctactcc tgtagcttat ttgacagtta tcaaggaggg gttgaaccgt
     1381 gaaatggtga ttaaggatgg agatgttctg gagaaatatc tggaagttga tactttcttg
     1441 tttgataaga caggaacaat cacaactagt tatcctatag ttgaaaaggt gttacctttt
     1501 ggagactata gtgaggaaga tattctcaga atcagtgcct gtcttgagga acacatttat
     1561 catcctattg ctaatgccat cgtcaagcaa gctgagatag agggaattga acatgaggaa
     1621 atgcatggga aactccaata tatcgcaagc aaggggatca aatctcatat agatggccaa
     1681 ccagttctta ttgggaatta tgtcttgatg caggatgagc agattcatat cagttcagaa
     1741 caaaatgctt taattgaaga gtacaagagt cactacaatc tcttattctt ggcttatcaa
     1801 aatgaattga ttggaatgtt ctgcattcat actcctttga gaaaagaagc aaaaacagcc
     1861 ttggataaac ttaaggcaca ggggaaaaaa ttgattctgg caacaggaga caccttgatt
     1921 aggacagagg aattagtcaa agatttgccc tttgatcagg tctatacaga cttgaaacct
     1981 gatggaaaat ttgagttagt agagaaactg cagaaagcag gtcacactat tttgatggtt
     2041 ggagatggat tgaatgactc agcggctcta actctatcag atatcggtgt ggtgatgaat
     2101 gagagtgcag atatttctaa gcagatgagt gatatcttat tgttagataa tcgtttggat
     2161 ttcttccaag agttggattc gttatcatca tctttgcaaa cactcatcaa gaaaaatatt
     2221 caagataccg ttgtcgtaaa tagtagtttg attggcttcg gcttatttaa ttggctcagt
     2281 ccttcaaatc tctctatctt acataatcta acaaccttgc gcattgtcct gcgtagcctg
     2341 tctattaaaa atagatagat gagagttatt aacagcaaaa aggagttacc tttctcgagg
     2401 ttaactcctt tgtttataga taaatgttga atagtttagt aagtcaatac aaagtatttt
     2461 ttcttcccac gacggataac agtcagttca ttttctaact tatcagcgtc actcaagaca
     2521 tagtcaagct cttggatgcg gtcgccgttt acgtagatgg ctccgttttg gacgtcttca
     2581 cgggcttggc gttttgagtt aactatacca gatgagacga gcagttccac gatattgttg
     2641 ttttcgtctg cctgtacttg gtagtttggc acaccacgaa gtccttgttt gagctctttg
     2701 acagaaaggt ttttgatgtt tcctgcaaag agttgctcag tgatgttaag tgcttctttg
     2761 taggcttctt ctccgtgaac aagtgtaaca acttcacgag ccaagacttt ttgagccaag
     2821 cgttcgtgtg gcgctgcttc aaattgttta cgaatatctt caatctcatc aagtgacaag
     2881 aaagtaaaga ttttcaagaa gcgaacagcg tcagcgtcca tcacgttcat ccagaattgg
     2941 tacatttcgt atggagaagt cttttcggga ttgagccaga cggcatttcc ttctgattta
     3001 ccaaatttct taccagttgc atctgtgatt agtggaacag tgataacgtg accagtcttg
     3061 tccgccttac gacgaagcaa ttcggtacca gctgtcatat ttccccactg gtcagaacca
     3121 ccgatttgaa gagtgacatt atggtcttgg ttaaggacga agaagtcata cccttgcatg
     3181 atttggtaag cgaactcagt gtaagaaatt cctgtttcga tccgtttttt aacagattcc
     3241 ttactcatca tgtagttgac cgtgaagtat tttccaatat cacggaggaa gtcaatgaag
     3301 ctgatgctgc caaaccagtc gtagttgttg accatgacag ccttgttttc gccattttca
     3361 aagtcaagaa aacgagaaag ttgtccttgg atagacttga cccagccatc tactgtgtct
     3421 tttgtttgga gactacgttc agcatctttg aaggacggat ctccgatgag acctgtagca
     3481 ccgccaacga gcgcataagg tttgtgacct gctagttgca agcgacgact tgtcaagatt
     3541 gcgacaaggt ggcctaggtg aaggctgtca gcagttggat cgtagccagt ataataagaa
     3601 acttgacctt cttctagggc tttacgcaaa gcttcttcat cagtcgtttg aaatatcaaa
     3661 ccacgctctt ttagctcatc aaaaatgtgc atgtgttttt tctccttttt aaaattattg
     3721 tttctaccta ttgtatcaca aacttgggca atagcctagc agaataggga agaaagtggc
     3781 tattttattg aaaatttccc ttttgtggta taatagataa agtgaggaaa tccatgcaaa
     3841 atcaattaaa tgaattaaaa cgaaaaatgc tggaattttt ccagcaaaaa caaaaaaata
     3901 aaaaatcagc tagacctggc aagaaaggtt caagtaccaa aaaatctaaa accttagata
     3961 agtcagccat tttcccagct attttactga gtataaaagc cttatttaac ttactctttg
     4021 tactcggttt tctaggagga atgttgggag ctgggattgc tttggggtac ggagtggcct
     4081 tatttgacaa ggttcgggtg cctcagacag aagaattggt gaatcaggtc aaggacatct
     4141 cttctatttc agagattacc tattcggacg ggacggtgat tgcttccata gagagtgatt
     4201 tgttgcgcac ttctatctca tctgagcaaa tttcggaaaa tctgaagaag gctatcattg
     4261 cgacagaaga tgaacacttt aaagaacata agggtgtagt acccaaggcg gtgattcgtg
     4321 cgaccttggg gaaatttgta ggtttgggtt cctctagtgg gggttcaacc ttgacccagc
     4381 aactaattaa acagcaggtg gttggggatg cgccgacctt ggctcgtaag gcggcagaga
     4441 ttgtggatgc tcttgccttg gaacgcgcca tgaataaaga tgagatttta acgacctatc
     4501 tcaatgtggc tccctttggc cgaaataata agggacagaa tattgcaggg gctcggcaag
     4561 cagctgaggg aattttcggt gtagatgcca gtcagttgac tgttcctcaa gcagcatttt
     4621 tagcaggact tccacagagt cccattactt actctcctta tgaaaatact ggggagttga
     4681 agagtgatga agacctagaa attggcttaa gacgggctaa ggcagttctt tacagtatgt
     4741 atcgtacagg tgcattaagc aaagacgagt attctcagta caaggattat gaccttaaac
     4801 aggacttttt accatcgggc acggttacag gaatttcacg agactattta tactttacaa
     4861 ctttggcaga agctcaagaa cgtatgtatg actatctagc tcagagagac aatgtctccg
     4921 ctaaggagtt gaaaaatgag gcaactcaga agttttatcg agatttggca gccaaggaaa
     4981 ttgaaaatgg tggttataag attactacta ccatagatca gaaaattcat tctgccatgc
     5041 aaagtgcggt tgctgattat ggctatcttt tagacgatgg aacaggtcgt gtagaagtag
     5101 ggaatgtctt gatggacaac caaacaggtg ctattctagg ctttgtaggt ggtcgtaatt
     5161 atcaagaaaa tcaaaataat catgcctttg ataccaaacg ttcgccagct tctactacca
     5221 agcccttgct ggcctacggt attgctattg accagggctt gatgggaagt gaaacgattc
     5281 tatctaacta tccaacaaac tttgctaatg gcaatccgat tatgtatgct aatagcaagg
     5341 gaacaggaat gatgaccttg ggagaagctc tgaactactc atggaatatc cctgcttact
     5401 ggacctatcg tatgctccgt gaaaatggtg ttgatgtcaa gggttatatg gaaaagatgg
     5461 gttacgagat tcctgagtac ggtattgaga gcttgccaat gggtggtggt attgaagtca
     5521 cagttgccca gcataccaat ggctatcaga ccttagctaa taatggagtt tatcatcaga
     5581 agcatgtgat ttcaaagatt gaagcagcag atggtagagt ggtgtatgag tatcaggata
     5641 aaccggttca agtctattca aaagctactg cgacgattat gcagggattg ctacgagaag
     5701 ttctatcctc tcgtgtgaca acaaccttca agtctaacct gacttcttta aatcctactc
     5761 tggctaatgc agattggatt gggaagactg gtacaaccaa ccaagacgaa aatatgtggc
     5821 tcatgctttc gacacctaga ttaaccctag gtggctggat tgggcatgat gataatcatt
     5881 cattgtcacg tagagcaggt tattctaata actctaatta catggctcat ctggtaaatg
     5941 cgattcagca agcttcccca agcatttggg ggaacgagcg ctttgcttta gatcctagtg
     6001 tagtgaaatc ggaagtcttg aaatcaacag gtcaaaaacc agggaaggtt tctgttgaag
     6061 gaaaagaggt agaggtcaca ggttcgactg ttaccagcta ttgggctaat aagtcaggag
     6121 cgccagcgac aagttatcgc tttgctattg gcggaagtga tgcggattat cagaatgctt
     6181 ggtctagtat tgtggggagt ctaccaactc catccagctc cagcagttca agtagtagtt
     6241 ctagcgatag cagtaactca agtactacac gaccttcttc ttcaagggcg agacgataat
     6301 tctctaaatg aagtggccaa tcaatggatt gccacttttt tcttttcgtg ttacaataag
     6361 ggtatgaatc agtatcagaa aaagattgtt aacggaaaaa tttattcgct cctatccggc
     6421 ttaatatggg gaatctgtgg aattttagga gagtacttct ttactcatta tcaggtgtct
     6481 tcgggctgga ttacctctat gcgtttgaca ctggcaggga gtcttgtact catttggtct
     6541 gcaatacaat taaaatcgca agtgctagat atttggcgag acaagaaaaa ttacctgccc
     6601 tttttagcct atgctatttt ggggattttt tcagttcagt attttttcta tctctgtgta
     6661 gaatactcaa atgctacgac agcaactatt ttacagttta ttagccctgt ctttatcctc
     6721 ttttacaatc gcttggttta tcaaaaacga gcgtcaaaaa gcgctgtttt ctatgttttg
     6781 gttgccatgc tgggtgtttg cttgatggcg acaaagggag atctctctca gttatccatg
     6841 acgccgctag ctcttataac aggtttgctg agtgccatgg gtgttatgtt taatgttatc
     6901 ttgccccaac cttttgctaa gcgttatggt tttgttccta cggttgggtg ggggatgatt
     6961 ttggcaggtt tgtttagcaa tgtcctctcg ccggtttatc agctttcctt tactcttgat
     7021 atttggagta tcttgatttg cctcattatc gctttctttg gaacggcttt tgcttttttc
     7081 atttccatga aggctgtgtc cttggtttct cctttggtgg ttgccgttat cagtgccagt
     7141 gaacctctct cttctgctct cttgagtgtt ttgttcttag gattagtagt ggattggtcc
     7201 ctccttctag ctatagcctt gattatttta cccatgatat ttttgtctat agaagaagcg
     7261 aaagaaagta gataaaaagt cttttcttaa ttctaaaagt gtgctatact agaatagtca
     7321 ataaaacacg gggagatagt tgtgaagagt gtttttaggt tgtatcggta ggggcttgct
     7381 tttaccgaca gcacgtttta tctcacaacc ctaaaaaatc atacctaaaa acagacaaaa
     7441 aggtttcaaa tttgaggcct tttttggtag aataggtatc attataacga accaggaggc
     7501 acctatgact gctacaaaaa tgaacgctca agaaattatc caatttatcg ccaatgctga
     7561 aaagaaaacc agtgtcaaag taacctttga ggggcaactc gcaactgctg tgcctagctc
     7621 tgttgtcaaa ctaggaaatg tcctattcgg agactggaag gacgtggctc cgcttcttga
     7681 aggtttggta gaaaatcaag attatgttgt cgagcaagat gctcgtaatt ctgcagttcc
     7741 tttgctagat aagcgtgcta tcaacgctcg tatcgagcca ggtgctatta tccgtgacca
     7801 ggtggaaatt ggtgacaatg ctgttatcat gatgggagct gttatcaata tcggtgctga
     7861 aatcggtgct ggaaccatga ttgacatggg tgccatcctt ggtggccgtg ccatcgttgg
     7921 aaaaaatagc cacgttggtg caggtgcagt tttggcaggt gtgattgagc cagctagtgc
     7981 tgaaccagtc cgtgtcggag acaatgttct tatcggtgct aatgcagtgg ttatcgaagg
     8041 agtccaaatc ggtagtggtt cagttgtcgc tgcaggagct atcgttaccc aagatgtccc
     8101 agaaaacgtg gtagtagcag gtgttccagc tcgtatcatc aaagaaattg atgcccaaac
     8161 ccaacaaaaa acagcgctag aggatgcgct tcgtaccttg taattgtaaa agtaaaaaag
     8221 aggcggaacc ctttttccag cctctttctg ctatatcgga ggacagatag atgttagatt
     8281 tgattcagac tagacgagct ttacaccaga ttccagagat tggcttggag gagttcaaga
     8341 ctcaggctta tttgctggat gtgattgaga aattgactac gggcaaggat tttgttcaaa
     8401 ttcgtacttg gcggacaggg attttggtct acctgcaggg aagtcagccg gagcgaacca
     8461 ttggttggcg gacagatatt gatggtctgc ctatcgtcga acaaacaggt ctaccttttg
     8521 cttctcaaca ccaaggtcgc atgcatgcct gtggtcatga ttttcatatg accattgcct
     8581 taggctgtct tgagcgcgcc cttgaggagc aacccaagaa taatctgctc tttctatttc
     8641 agcctgctga agaaaatgaa gctggtggta tgctcatgta tgaggacggt gcttttggag
     8701 attggttgcc agatcaattc tatggtctcc atgttcgtcc agatttgaaa gtcggccaga
     8761 ttgcgactaa tactcataca ctctttgcag gaacttgcga ggtgaagatc cgtttcaaag
     8821 gcaaaggtgg ccacgcagct tttcctcatg aagccaatga cgccttggtg gcggctagtt
     8881 actttgtgac ccaagtgcag tcagttgtta gccgcaatgt caacccaatc gagggagcag
     8941 tggtgacctt cggcgttttt caagctggaa ccaccaacaa tgtcatcaca gacacagctt
     9001 ttttacatgg aactattcgt gccttgacac aggacatgag tctcttggtg caaaagaggg
     9061 taaaaacagt tgcagaaggg gttgcagctg cctttgatat ggaagtcgaa gtggaactca
     9121 aacaaggtgg atacctacct gttgagaaca atccagcctt ggcgcgtgaa ctgatggact
     9181 tctttgacga aaaagacgga atcgagttga ttgatatcga acctgctatg acaggcgagg
     9241 actttggtta tctcctttca aaggttgatg gcgtgatgtt ttggctaggt atcgatagtc
     9301 cctacgctct tcatcaccct caaatgagtc ctaaggaaga agtcttagct attggggtgg
     9361 ctgcggtgtc tagtttcctg aaaaagaagg cagcagaata gagggcttgt ctatgaaatc
     9421 ggaattacgc aagcaagtct tgcatgaaat gaaggcttta tctcaagaac aaaaacaggc
     9481 tatagaccaa gctttaaccg agcgaatttt acaacacccc ttttatcaag aagccaaggt
     9541 catcgcaacc tacctctctt tttctcatga gtttcaaacg cgggaactga ttgagcaggc
     9601 gctgaaggac ggcaagaagg ttttgacacc taagacctat cccaaggggc gcatggactt
     9661 tgtagtctat gatccgcagc agttggtaaa aactgccttt gccttactgg agccacaggg
     9721 agatttggaa gtggtggatg tatctcagat tgatttgatt catgttcctg gcctggcttt
     9781 tacgacggat ggatatcgga ttggatatgg tggaggttat tatgaccgct atctggaaca
     9841 tttttctggt catactttga gtacggttca tccttgtcaa attcaggact ttatacctga
     9901 aaaccatgat attcctgttc aggaggtatt aattgatgaa ggaaatcttt gatagacgtt
     9961 accctgtgac gagtttcttc ctcttagtga cggccttggt atttttacta atgttggtca
    10021 ctgcaggcgg aaactttgac agggcagata cattatttcg atttggagcc atgtatgggc
    10081 cagctattcg cctctttccc gagcaggttt ggcgtctctt gtctgccatt tttgttcata
    10141 ttgggtggga acatttcatt gttaatatgc tttcacttta ttatcttgga aggcaggtag
    10201 aggagatttt cggctctaag cagttctttt ttctctatct cttatcagga atgatgggca
    10261 atctctttgt ttttgtattt agtcctaaat ccttagcagc aggcgcctct acctctcttt
    10321 atgggctatt tgccgcgatt attgttcttc gctatgcaac tcgcaatcct tatatccaac
    10381 agctagggca atcctatctg acactttttg tggttaacat tattggaagt gttctgattc
    10441 caggaatcag cctagcaggc catatcggtg gtgcagttgg tggcgcattt ctagcagtta
    10501 tctttccagt tagaggagaa aaacggatgt ataacaccag ccagagatta ggagcggtag
    10561 tcttgttcgt aggactcgcc attttgcttt tctacaaggg aatgggaatg tgacgaagtt
    10621 gaaagaaaat ttgtgtaatg aaaaaagata aggcattttt tgaaccttat ctttttattt
    10681 tattccttct cagtcaattc ttttccaagt tggatgaggt aattcttcaa atcatctttg
    10741 acttgtggat gtttgagggc gtagtcaata gatgttttca taaagccaaa cttatcccca
    10801 acgtcgtaac gagctcctgt aaattcacgg gcaaaaacac gctgtgtttt attaagagta
    10861 tcgattgcat cagtaagttg gatttcattt ccagcaccag gagcttggtt ttcgaggatt
    10921 tggaaaattt caggcgtgag gaggtagcgt ccgataatag caaggtcgct aggagcgtcc
    10981 tctggagctg gtttttcaac aaaggtttca acactgtaaa gaccatcttt tccttcgcct
    11041 tgcggagcaa taaccccgta agcagatact tcgtcatgag ggactggcat gacagcgata
    11101 gtagacgcgt gggtacgctc gtagtcatcc atgagttgtt tggtaagtgg aacagccttt
    11161 tcgtctgtga tatccatcaa gtcatcacca agcataacga caaaaggttc atttccgacg
    11221 aaagccttgg cttgcaaaac agcatctccg agaccgcgtg gatgagtttg gcggataaaa
    11281 tgcagccgca tgtcagttgt tttatcaact agcttcaaaa gatctgtttt ccctttttct
    11341 ttgaggttat attccaattc gaagtttgaa tcaaagtggt cctcaataga acgttttgat
    11401 ttaccagtga caactagaat atcttcaata cctgatttga gagcttcttc cacgataaac
    11461 tggatagttg gtttgtctac gattggcaac atttctttgg caagggcctt ggttgctggt
    11521 aaaaatcgag ttcctagtcc agcagcaggg atgactgcct ttctaacttt tgatgtcata
    11581 aaaatccttt ctatagaggg ttaagaccac tcattttctg ctttaaattc attgttcatg
    11641 atgtcataaa tggcatcttt gatattggtt ccgtgataaa taacttggta aatagcctgt
    11701 gtaatgggca tatagactcc aagttcttgg gctagttcat aggctgctcg agtcgttgaa
    11761 attccttcga ttaccatgcc catattagct tctatatcag ctagggattc tcctcgtccg
    11821 agagcatctc cagctctcca gttacgagag tggatggaag ttcccgttac gatcaaatct
    11881 cccacaccag ataagccgct ataggtcaat ggactggccc cgagtgctac ccctaggcgg
    11941 gtgatttctg ctaaacctcg agcgatgatg gctgccttag cattatcacc aaatccaaga
    12001 ccatgtaaag ctccagcacc gacagcaata atatttttaa gagcaccagc agtttcaacc
    12061 ccgataacat ccgtattggt ataaagtcgg aagtagtgat tactaaatag cttctgaacg
    12121 tattgagctg tttgtaaatc tttagaagca gcagttatta aagttaggtc acgcacaatg
    12181 gtctcttctg catgactagg ccctgaaaca acgacgatat cactacggag atgttcagga
    12241 atttcttctt caagaatggt tgataatcgt ttatggctat caggttctaa tccctttgat
    12301 gcgtgcatga tgataacctt atggtccaag gtttgtgcaa cttgctgggc aacaagtcgt
    12361 gtcacttttg ttgggacaac aaacaaaatc gcatccacat ctttcaatgt ttctgctaag
    12421 tcggtgtagg caatgatatt ttcgtctaga acgacatctt taaagtagtg cttattagta
    12481 tggtgtgtat taatttcatt gatttgctcg ggaagatttc cccaaatacg tacctcgtgt
    12541 ccattgtcat ttaagacttg tgaaagggcg gttccccaag aaccaggccc caagacggcg
    12601 acggtttgtt tttccatgat ttcctccttg atagagttct ttctcttatt ctatcatatt
    12661 ctaggggatt ttgcacttgc attactgggg tatggtggaa aaatacacaa aaaccgagac
    12721 gaagggataa actcttagtc tttagttaaa gtgagaagaa atctgataga tagtttgatt
    12781 cagtatatgc ttataataga ggtaaacaac tcaggaggtt cttgtaggtt gcgagagttt
    12841 ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca gtcgttcaga attttgtggt
    12901 gatgagtctg aattaagtat ccgtcaatta attagaattg aaaatggaga atccagacca
    12961 atactaacaa agttaaaata tattgctgaa cgtttggagg ttgaagatta caagttgatg
    13021 ccaagttata tagagttgga taaggaatac ctagaattga agtatttctt gatgaggact
    13081 cctacatacg aagatgaaac tatcgcccaa aagaaagaga gtgtttttga taagattttt
    13141 gaagagtatt atgataggct acctgaggaa gaaagattta tcatcccaaa ttattcatat
    13201 ctggcactaa cgaactacac agttcaaaaa ttaccagaaa agctagttga aatactgtcc
    13261 ttctggtgat tttgcttaat ttctgatgtt cgagacatga ttgaggagat ttgtcttgat
    13321 tttttcctaa aaaagggcag actcctccct tggtttgatg ctcgattttt aaaataaggt
    13381 gacttattct gttaggcatg ttttcccctg ccctctagct ctatatggaa cagggagtat
    13441 cagatttatc tggcagattg tatcaaataa ggcttgaaac tgttttgaat aggcatatag
    13501 acttgagaat ttgagaatat gtcgtctagc agtagagaca tattttccgg caatatgaag
    13561 gaagagacgt cgaaaacgct tgatagtcaa ggcctttact tcatcaccag ctagctgctt
    13621 taaaaagagg tagagattat aagccagaca gcccatcatc atacgtactt cattcttaat
    13681 catggtcgaa ctatctgtct tatccccaaa gaatcctgct ttcctttctt tgataaagtt
    13741 ttcagcattc cctcgttcac gatagaattg aaacagctcc tcactatcta cactgcttaa
    13801 attggtcaaa tgaacttttt tacaaccata aaaaagagca cttaatcgta ctcttctctt
    13861 ttaaggatta gttgaattaa ttcaaatcca ctagttctcc tgtttctagg tagactaggc
    13921 gttcacaaat gttagcaatg taatcagcga agcgttccag atgccctatg atataaaggt
    13981 attgagttcc attgggaatt gaggtttctt ggtctttcat aagtccaatg atttcctttg
    14041 ataaggcata ataatattgg tcaatctgtt catctttttg agcaatacta atagcttttg
    14101 aggcttggtg caaaggaaag gcaaccaata aatcggctag catgctgagg gataatttac
    14161 ccatttggtg taactgttct tcgtcagggg ctagttgatt ttcttttagt tgcaaaacag
    14221 ctttggcaat gcctgccata tggtctccca tacgttcaag gtctgaacaa gaagacatga
    14281 tgctaatcac aaatcgaagg tcagacactt gtggctgctg caaggccaac aaacgggcac
    14341 aggtcaattc gatagcgctt tgaccttggt tgatagcatg atccttattg ataattagct
    14401 ctgccatctc cttgtctttg gaggctaagg ccagtaaggc ttttgaagct gtttcaagga
    14461 caagttgccc tagtcctaaa aaggattgtt ctaattcatg caattctaag tcaaattgat
    14521 ttctcatagg tttttccttc cttatccgaa ccgtcctgaa atatagtctt ctgtgcgctg
    14581 atcttttgga ttggtaaaca cgtcaacggt atctccaaat tcgcaaattt ctcctgttaa
    14641 gaaaaaagca gttttatctg aaatacgtga agcttgttgc atgttatggg taacaatgat
    14701 aatcgtataa tcctttttta gttgctgaat gaggtcttca atttttaaag tggagatagg
    14761 gtctaaggct gaagtcggct catccattaa cagaatatca ggttctactg ctaaagctcg
    14821 cgcaatgcaa aggcgttgct gctgaccgcc agataaggac atggcactct ttttaagatc
    14881 atctttgact tcttcccaaa tggctgcccc ttttaaagat ttctccacta aggcatctaa
    14941 ttgttttttg tctcgaatac catgtgtcct tgggccataa gccacgttat catagataga
    15001 catggcaaag ggattaggct gttgaaaaac catccctaca cgcttacgta gctgattaag
    15061 gttgaatttg ctactataaa tatcttgctc atctaagagg acttggcctt caatatggca
    15121 agaaggaacc aaatcgttca tccggttaag ggtttttaga aaagttgatt tgccacaacc
    15181 agatgggcct atcaaggcag taatctgtct ttctggtaat tgaatcgaaa tattttttaa
    15241 ggcttgaaaa tccccgtaaa ataagtctag gtgtctgact gaaaatgttc ccatactagg
    15301 aagctccttt cacaagtttt cgagataata agcttgatag agtatttatc attaaaacag
    15361 taataatcaa aatcacaccg gtagcatagg cttcattgac atgtagcccc tcacttgaca
    15421 gcatatacat atgcagggct agagaacgac ctgaagacat gagactactt ggcgtattgg
    15481 tagaggtacc taatgtatac atgagggcag ctgtctcacc aacgatacgg ccaatagcta
    15541 gtatcactcc agctaaaata cctggcatgg caactggtag aacaattcta aaaacagtcc
    15601 gtaacttacc tgccccaagt ccataacttg cttgacgcat gctatcacta acagataaaa
    15661 gggcttcttc tgttgagcga ataatgactg gcaacaccat gatgactgag gttaagattc
    15721 ctgataacag agagtattga aaacctaaga agactacaaa gaagagcatg ccaaacagac
    15781 caaaaacaat ggaaggaatc ccagacaagg tatctgaggc caatcgcatg attttaacac
    15841 aaagggaatc tttttttgta tattccacaa gataaaaacc agcaaaaatc cctatgggca
    15901 aggctaaaag aagagcacca aagaccagaa taacggtgga aataatcgct ggcataaggg
    15961 aaatgttctc agaagtataa gtccaagaaa agagggatag acttagatga ggtaagcctt
    16021 tgatgaggat aaaaccaatg attaaaaaga gagagccaaa ggttaaagct gaaaaacaat
    16081 aaacgagaag ttttagcagg tatttactca taagatgatt ttcctttcaa gtaggcaaag
    16141 taggcattaa tcaagagaat aaggaaaaag agaactgctg aggttgcaat aagggcttcc
    16201 ctatgctgac ctgatgcgta agccatttcc agaacaatat tggttgttaa ggttctggtt
    16261 cctgaaaaga gtccacttgg aataatcggc tggttgcctg ccaccaaaat aactgccatg
    16321 gtttcaccta ctgcgcgacc gattcctaaa ataactgctg ataaaatacc agatctcgca
    16381 gctggcaaga tgacactaaa aatactccgt tcatgactag ctcctagagc caagctacca
    16441 gaataatacg ttttgggaac tgttcggata gcagattctg acaaactgat aatggttggc
    16501 aaaatcatta ttcctaatag taacgaagcg gttaggacac tcatgccatt tcctaaaaag
    16561 cttctaatcc aaggcaccaa taattgtagg ccgaaaaaac cataaacaat agatggaatg
    16621 gccgccatca agttgatagc tgattttaag aagccataga cgggctttgg acaataataa
    16681 accataaaca ccgatgtcaa gatgcctgtt ggcaccccaa tcacaatcgc tcctaaggta
    16741 attaataagg aaccaacgat cattggtaaa ataccatagc ttgccggaat gttcgttggc
    16801 gaccaatcac tgcctaataa aaaacgggca aagccgtagt tagccatgaa aggtaagcca
    16861 ttactaaaaa taaagaaaca gattagcaaa atagctacaa cagctactgt tgcactcatg
    16921 aaaaaaattg ccctaaaaac tgcttctttg aaggcttgtt ttgtcacatc ttgtcctttc
    16981 tagtgaagaa agtaagggag atacgacacc tccctacttg ccttctttat cttattgtac
    17041 gatgaaacgt ctgcatctct ttagagattt atggagcaaa cattttattt aatcttgtcc
    17101 caggtggtta atttgccact aaaaacgtct gcaagttcag ccatactgac ttggcttgcc
    17161 ttattgtcat tattgaccac aacagcaata ccgtctaaag caatagcatc atgggtgaga
    17221 ctcttacctt cttcaggagt taattcccta gaaaccatac caatatcagc ggttttctcc
    17281 ttaacagcgg taatacctgc tgaagaccca ttagaggtaa tatcaatcgt aacttctgga
    17341 ttttcttttt tataagcttc tgctaatttt tccattaaag aagatactga agtggaacct
    17401 acaacagaca acttgcctga taagtgttgg cttgtatatt ctgtggtttc agttttagct
    17461 tcaataaatt tattatctgt gaccacttgt tgaccttgtt tggagtggat aaagctgata
    17521 aaatcttgac ctagcttgga aagattagaa gaccaaacaa tgttgaaggg acgttgaaga
    17581 gggtattcac catctaaaac tgtgtctcga ctagccttga caccatcaat ctctaaagct
    17641 ttgacagatt tcgttaaaga tcccaaggag atgtagccga tagcattagc attcccttga
    17701 actgctgaga gaacaccttc tgtactattt tgaatcacag ctgttttggc agtgttgtca
    17761 atttttttat caccgtcttt tttgagaatc cctgtgattt ctgtgaaggc accccgtgtt
    17821 ccagagccat tttctcgtga aatcacctca atcgttcctg aagctgactg tttggaagca
    17881 gctgactgat tgccacaggc aacaagccca aatcctgata agccaatggc tgcaagagta
    17941 agcatttttt tgaatttcat aataatcacc tttatctcta tgtatttttc ttgtgtaggc
    18001 ttactacatt tatagtctaa caagtctttg taaaggttta tccctgattc atgtaaagat
    18061 tgtgtaaaga atcaaaaaaa gccacttttg aaaaatggct gctcctaaaa atagctttaa
    18121 aaattattag tcctgtgcga aagattggtt aggaagaaaa atcgtgaagc aactgcctct
    18181 gccaagctga ctcgtcaccg tgacttggcc acctaataat tgactgagtt ctttgacaat
    18241 ggcaaggcca agaccagtgc caccagtttg tctgcttcga cctttattaa ctcggtaaaa
    18301 acgttcaaaa atacgatcct gctctaattg actaatacca atccctgtat ctgatacaga
    18361 aatcttaatg ccttcgttca ccttttgggt cttgacctca atttttcccc cttgttcagt
    18421 gtaacggatg gcattggata aaagattgag taagatttgg gaaagtaatt gactatctga
    18481 tacgagggtg acatcatctg gcacctgcac ctttagctgt aaatccttct tcttgagctg
    18541 aggttgcaag ctttgagtca aatcctgtac aaattctgcc aaagaaaggg tcgtccattg
    18601 tataggcatt tgttgagcct tagataaggt aagaagatgc tcaacaatat gctcaagacg
    18661 caagctttct ttgtaaataa tgtctagaaa gtcatccttg agcgcttctt cttcagctga
    18721 catcccctta atggtttcag caaagccctt aatcgaagta actggtgtcc tcaattcatg
    18781 ggaggcattt gagacaaagg ctaaatttaa cttttcataa gttctaatcg ttgttaaatc
    18841 atatagcaag acgagcacag cttccacaga ttgggtgggg ctaaaaacgg gaactgctgt
    18901 cacttctaaa atcaagtcac cctcatgaaa cccacttact tcttgtttta accttgtttt
    18961 ttgatcaaag gcttggtgaa ctaaatttcg aatatccatc cgtttgaggt catcaagtga
    19021 acttatgtcg ccgtccacat cgggaaaata atgaggcaga gagcgactgg ataataacat
    19081 ctgaccttga gcggaaacta aaaacgtccc catggttagg tgcgacagaa gaacctccat
    19141 tgtttcggct aggtccttgt attgctgatc ctgttgggag actttggttt ttagggcaga
    19201 cacatactga gccaaagact ttaagtcttc ttgccctttt tctaaaaagt attcactact
    19261 ggtcaagaga ggttggtgca aggtctcaaa agcaacttcc catttccaaa ggcaaaagag
    19321 ccagtagcca ccttgtccca aagaaagggc tagaagaaag agaccgatgc ctttactgat
    19381 ccaagttaat gccatccctg caatcagaat gaggctaaca cttagattga ctagccaaaa
    19441 ttgaaggtag cgtttcatct ataactcctt gaacttataa ccataacccc gaatggttcg
    19501 aataaattga ggggctttag gattgtcttc aattttttcc ctcaacttac caatatgaac
    19561 gtccaccaaa cgtgtttcct gcccaaagtc atacccccag atacgttcca aaagacgctc
    19621 tctagtcagt gtcatgttgg gatgtttcat aagatagagc aagagttcaa attcttttgg
    19681 ggtcaaactc agtaacttat tcgccttgta gacttcatga cgctcagggt atactttcaa
    19741 ggtcccaaat agccaagaat cgtcagcgat attatctgaa tcatctcctt cttgttctcc
    19801 tttagttcgc ctgaggacag ccttgacacg cgccagcaat tctctagggc taaaaggttt
    19861 ggtcaggtag tcatcagccc ctaattccaa ggccaaaacc ttatcaaatt catcactttt
    19921 cgcagaaacc atcataattg gagttttgac gcctttggct ctcagccgct tacaaacttc
    19981 catgccatct aattgtggta acatgatatc aagcaagata aaatcaaagg gttctgtttc
    20041 tgccaaagct aaggccttcc gtccatttgt taccaattga gtagaaaagc cttccttact
    20101 taaatggtag tcaagcaatt tcagaatgtg ttcttcatca tccactaata agacttgttt
    20161 tgtcatctat tatctcctat tggtaacatt ataacacaat tatcagaaat cctaacattg
    20221 ctaaatcaga ttaaatttgc ttatcaagac tagtatctgg tcaaacgctc aatcatctcc
    20281 ttgtgctctg gataggtcgc cagtagatct accctttcaa ataattcaaa atcctcaaat
    20341 tcaaaaccag gagcaacaag acaagaaacc agagcatcat ccttatcaac tgttgatccc
    20401 caaatagtgc ccttaggaac acagtagtga agttgttgcc ctttggatat gtccaggcct
    20461 aaagtgactg cttcgtagtg accatctgct gtaatcatgt gaacagtaag tggggattct
    20521 gcatgaaaat accagatttc atctgctgtc aatcggtgaa aatgtgaagg attcgtttct
    20581 tcttaataag aaataaatac tggtataaag cgcccttccc ttaccagcaa ggtttatagt
    20641 gtctgaagct ttttttgttt gtctaaaata gccaccttca acatggggag ctaactctag
    20701 agttcttatc aagtcttctt tatccgtcgg agccaatggg ttgaagtaac tcttgttcaa
    20761 aggtggtgtt acgatttcaa gaactcctct cagctctgag gacacgataa tgattgatgc
    20821 gacggaagta aaaatcaatc gccctaaaaa aacaattagc gaataattct ggtaaaaaga
    20881 aatttcacgc tatgaaggct caagcgattg tcacaagtca agggagaatt gtttctttgg
    20941 atatcgctgt gaactattgt catgatatga agttgttcaa aatgagtcgc agaaatatcg
    21001 gacaagctgg taaaatcttg gctgacagtg gttatcaagg gcccatgaag atatatcctc
    21061 aagcacaaac tccacgtaaa tccagcaaac tcaagccgct aacagttgaa gataaagcct
    21121 gtaaccatgc gctatctaag gagagaagca aggttgagaa tatttttgcc aaagtaaaaa
    21181 cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga ttacgaatga
    21241 atttgattgc tggcattatc aatcatgaac taggattcta gttttgcagg aagtctatta
    21301 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggcc
    21361 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
    21421 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
    21481 aggacataaa tcgatcatat ttatgtccta aaactagtga agcgcctagc caaagtccga
    21541 ataggatttg gcgttagtta cttagattgc tttgcaatca agtaactttg gcgatttaca
    21601 tcttctctgg cgcttctact ccaagcaagc gaagggcttc tttgagaacg actgcggttg
    21661 cgtagctgag ggctagacgg ctgtcgcgtt ctgggctttc atccaagata cgtgtatgtg
    21721 catagtattt gttaaaggat tgagccaggc taattgcaaa tttagcaatg atagaaggtt
    21781 caaagttatc tgccgcacgg ttgataatac gtgggaagtc ttgaatgagt ttaatgattt
    21841 cccagctttc agtatcattc aagctatagt tgccagctgt ttctggtttg aaatcggctt
    21901 tgcgtaagat agattggata cgagcgtagg catattgaac gtaaggtcca gtttcaccct
    21961 cgaaggatac catagcttct aggtcgaagt cgtatccatt tgtacggtcg gttttgaggt
    22021 catagaattt aatggctcca accccaacag catgtgctac ttggtctttg ttttctagtt
    22081 caggattttt agcctcgatt tggaccttgg cacggctaac agcctctgca atagtaggct
    22141 ctagcaagat gacattccct ttacgagtag agagcttctt cccttctttt gtaaccagac
    22201 caaaaggaac gtgggtaatg tcgtcactcc aatcgtagcc catctcttgc aagacagctt
    22261 tgagctgttt aaagtgggca gattgttctt gaccaacgac gtagatagat ttagcaaatt
    22321 ggtattcatt tttacggtag agggctgcag ccaagtcacg tgtgatatag agagttgcac
    22381 catcagactt cttgatgagg gctggatgtt caattccgta tttctcaaga ttcacaactt
    22441 gggcaccttc tgattcaaga agtagtcctt tttcagaaag aatgtctaca actgcatcca
    22501 tcttatcatt gtagaaggct tctccgttat agctgtcaaa ttcaaccttc aattcattgt
    22561 aaaggcggtt aaattccact aaactttcat cgcggaacca ttgccaaaga gcgagagctt
    22621 cctcatctcc attttcaagt ttacggaacc attcgcgcgc ttcttcatcc aagctagggt
    22681 cattttcagc ttcagcgttg atgcggacat agagtttaag gagttcatcg attggatgag
    22741 cttttacagc ttcttcgtcg ccccattttt tgtaggcaac aatcaacatc ccaaattgtt
    22801 taccccagtc tcccaaatgg ttgaccttga ccgtttgata accgattttt tggaaaatat
    22861 gtgacaagct atctccgata acagttgaac gcaggtggcc aatagaaaat ggtttagcga
    22921 tattcggact agacatgtcg ataacaacat tttcttgttt accaatattt tggtcagcat
    22981 agtgttcttt ttcagtggta acagcttgca atacttgagc agaaatggca gatttatcaa
    23041 ggaaaaagtt aacgtaaggt cctgttgcga caactttttc aaaggcttgg ctgttcattt
    23101 tttcagccag ttcagccgca atcatttgtg gtgctttacg ttcgactttt gcaagagaaa
    23161 aagcagggaa agcaatgtct cccatttctg agtttttagg ggtttccagt aactttaaaa
    23221 tagcctcttg gtccaggcta tcaatgatgc tagataattt gctagcaatc aattcttttg
    23281 tattcattaa gagctccttt ttggactttt ctactatttt atcacaattt taaagaaaga
    23341 agaaaaaatt tttgaaatct cctgtttttt tggtataata tggttataaa tatagttata
    23401 aatatgcacg caagaggatt ttatgagaaa aagagatcgt catcagttaa taaaaaaaat
    23461 gattactgag gagaaattaa gtacacaaaa agaaattcaa gatcggttgg aggcgcacaa
    23521 tgtttgtgtg acgcagacaa ccttgtctcg tgatttgcgc gaaatcggct tgaccaaggt
    23581 caagaaaaat gatatggtgt attatgtact agtaaatgag acagaaaaga ttgatttggt
    23641 ggaatttttg tctcatcatt tagaaggtgt tgcaagagca gagtttacct tggtgcttca
    23701 taccaaattg ggagaagcct ctgttttggc aaatattgta gatgtaaaca aggatgaatg
    23761 gattttagga acagttgctg gtgccaatac cttattggtt atttgtcgag atcagcacgt
    23821 tgccaaactc atggaagatc gtttgctaga tttgatgaaa gataagtaag gtcttgggag
    23881 ttgctctcaa gacttatttt tgaaaaggag agacagaaaa tggcgataga aaagctatca
    23941 cccggcatgc aacagtatgt ggatattaaa aagcaatatc cagatgcttt tttgctcttt
    24001 cggatgggtg atttttatga attattttat gaggatgcgg tcaatgctgc gcagattctg
    24061 gaaatttcct taacgagtcg caacaagaat gccgacaatc cgatccctat ggcgggtgtt
    24121 ccctatcatt ctgcccaaca gtatatcgat gtcttgattg agcagggtta taaggtggct
    24181 atcgcagagc agatggaaga tcctaaacaa gcagttgggg ttgttaaacg agaggttgtt
    24241 caggtcatta cgccagggac agtggtcgat agcagtaagc cggacagtca gaataatttt
    24301 ttggtttcca tagaccgcga aggcaatcaa tttggcctag cttatatgga tttggtgacg
    24361 ggtgactttt atgtgacagg tcttttggat ttcacgctgg tttgtgggga aatccgtaac
    24421 ctcaaggctc gagaagtggt gttgggttat gacttgtctg aggaagaaga acaaatcctc
    24481 agccgccaga tgaatctggt actctcttat gaaaaagaaa gctttgaaga ccttcattta
    24541 ttggatttgc gattggcaac ggtggagcaa acggcatcta gtaagctgct ccagtatgtt
    24601 catcggactc agatgaggga attgaaccac ctcaaacctg ttatccgcta cgaaattaag
    24661 gatttcttgc agatggatta tgcgaccaag gctagtctgg atttggttga gaatgctcgc
    24721 tcaggtaaga aacaaggcag tcttttctgg cttttggatg aaaccaaaac ggctatgggg
    24781 atgcgtctct tgcgttcttg gattcatcgc cccttgattg ataaggaacg aatcgtccaa
    24841 cgtcaagaag tagtgcaggt ctttctcgac catttctttg agcgtagtga cttgacagac
    24901 agtctcaagg gtgtttatga cattgagcgc ttggctagtc gtgtttcttt tggtaaaacc
    24961 aatccaaagg atctcttgca gttggcgact accttgtcta gtgtgccacg gattcgtgcg
    25021 attttagaag ggatggagca acctactcta gcctatctca tcgcacaact ggatgcaatc
    25081 cctgagttgg agagtttgat tagcgcagcg attgctcctg aagctcctca tgtgattaca
    25141 gatgggggaa ttatccggac tggatttgat gagactttag acaagtatcg ttgcgttctc
    25201 agagaaggga ctagctggat tgctgagatt gaggctaagg agcgagaaaa ctctggtatc
    25261 agcacgctca agattgacta caataaaaag gatggctact attttcatgt gaccaattcg
    25321 caactgggaa atgtgccagc ccactttttc cgcaaggcga cgctgaaaaa ctcagaacgc
    25381 tttggaaccg aagaattagc ccgtatcgag ggagatatgc ttgaggcgcg tgagaagtca
    25441 gccaacctcg aatacgaaat atttatgcgc attcgtgaag aggtcggcaa gtacatccag
    25501 cgtttacaag ctctagccca aggaattgcg acggttgatg tcttacagag tctggcggtt
    25561 gtggctgaaa cccagcattt gattcgacct gagtttggtg acgattcaca aattgatatc
    25621 cggaaagggc gccatgctgt cgttgaaaag gttatggggg ctcagaccta tattccaaat
    25681 acgattcaga tggcagaaga taccagtatt caactggtta cagggccaaa catgagtggg
    25741 aagtctacct atatgcgtca gttagccatg acggcggtta tggcccagct gggttcctat
    25801 gttcctgctg aaagcgccca tttaccgatt tttgatgcga tttttacccg tatcggagca
    25861 gcagatgact tggtttcggg tcagtcaacc tttatggtgg agatgatgga ggccaataat
    25921 gccatttcgc atgcgaccaa gaactctctc attctctttg atgaattggg acgtggaact
    25981 gcaacttatg acgggatggc tcttgctcag tccatcatcg aatatatcca tgagcacatc
    26041 ggagctaaga ccctctttgc gacccactac catgagttga ctagtctgga gtctagttta
    26101 caacacttgg tcaatgtcca cgtggcaact ttggagcagg atgggcaggt caccttcctt
    26161 cacaagattg aaccgggacc agctgataaa tcctacggta tccatgttgc caagattgct
    26221 ggcttgccag cagacctttt agcaagggcg gataagattt tgactcagct agagaatcaa
    26281 ggaacagaga gtcctcctcc catgagacaa actagtgctg tcactgaaca gatttcactc
    26341 tttgataggg cagaagagca tcctatccta gcagaattag ctaaactgga tgtgtataat
    26401 atgacaccta tgcaggttat gaatgtctta gtagagttaa aacagaaact ataaaaccaa
    26461 gactcactag ttaatctagc tgtatcaagg agacttcttt gacaattctc cacttttttg
    26521 ctagaataac atcacacaaa cagaatgaaa aggagctgac gcattgtcgc tcccttttgt
    26581 ctatttttta aggagaaagt atgctgattc agaaaataaa aacctacaag tggcaggccc
    26641 tggcttcgct cctgatgaca ggcttgatgg ttgctagttc acttctgcaa ccgcgttatc
    26701 tgcaggaagt cttaggcgcc ctccttgctg ggaaatatga agctatttat agtatcgggg
    26761 cttggttgat tggtgtggcc gtagtcggtc tagttgctgg tggactcaat gttgtcctcg
    26821 cagcctatat tgcccaagga gtttcatccg accttcggga ggatgccttc cgtaaaattc
    26881 aaaccttttc ttatgctgat attgaacaat ttaatgcggg aaatctagtc gttcgaatga
    26941 caaatgatat caaccagatt cagaacgttg tcatgatgac cttccagatt cttttcagac
    27001 ttcccctctt gttcatcggt tcgtttatcc tagcggttca aaccttacct tctctgtggt
    27061 gggtgattgt tctcatggta gtcttgattt ttggtttgac tgctgtcatg atgggaatga
    27121 tggggcctcg ttttgccaag tttcaaaccc ttcttgagcg catcaatgcc attgccaagg
    27181 aaaatttacg tggcgttcgt gtggtcaagt cctttgtcca agaaaaagag caatttgcta
    27241 agtttacaga ggtctcagac gagcttcttg gtcaaaacct ttacattggt tatgcctttt
    27301 cagtagtgga accctttatg atgttggttg gttacggggc ggtcttcctc tctatttggc
    27361 tggtcgcggg aatggttcag tcggatccgt ctgttgttgg ttccatcgct tcttttgtta
    27421 attacctaag ccagattatc tttaccattg ttatggttgg atttttggga aattctgtca
    27481 gccgtgccat gatttccatg cgtcgtattc gagaaattct tgacgcagag ccagctatga
    27541 ccttcaagga tatcccagat gaagagttgg ttggaagcct tagctttgaa aatgtgacct
    27601 ttacctatcc aatggacaag gaaccgatgc tgaaagatgt gagctttact attgaacctg
    27661 gtcaaatggt tggtgtagtt ggagcgactg gtgcaggaaa gtcaaccttg gctcaattga
    27721 ttccacgtct ctttgatcca caggaagggg ctatcaaaat cggaggtaag gatattcgag
    27781 aagtgagtga aggaaccctg cgtaaaacag tttccatcgt tctccaacgt gccattcttt
    27841 ttagtggaac gattgcagat aacttgagac aggggaaggg aaatgctact ctatttgaaa
    27901 tggagcgcgc agccaatatt gcccaggcta gtgaattcat tcatcgtatg gagaaaacct
    27961 ttgaaagtcc agttgaagaa cggggaacca atttctctgg tggacaaaaa caaaggatgt
    28021 cgattgcgcg tgggattgtc agcaatccac gtattctgat ttttgatgat tcgacctcag
    28081 ccttggatgc caaatcagag cgcttggtgc aagaagcttt gaataaggac ttgaagggga
    28141 cgacaaccat tattattgct caaaaaatta gctcggttgt ccatgcagac aagatcttgg
    28201 ttctaaatca aggacgattg attggtcaag gcacgcatgc agacttggtt gccaacaatg
    28261 ccgtttaccg tgaaatctat gaaacacaga aatgaaagac aaactataag aaaagtcaat
    28321 agttttatct aaactatttc ttatttcaat ttgatgattt ggcgatgatt ttagagcacg
    28381 gcaaaaagct attgaaaaag tccatttttt caaaggtaat cctgtgttaa tttcagaaat
    28441 tacatcactt tttgttcgtc aaatggcagc tcttttttag gatataaaac agggttcgga
    28501 taagtttttt tgcaaggtgg atgatgg
//
LOCUS       DAVMEI010000010        28399 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVMEI010000010 DAVMEI010000000
VERSION     DAVMEI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.010"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..536
                     /locus_tag="VYQ24_000359"
     CDS             24..536
                     /locus_tag="VYQ24_000359"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1967544.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            718..1662
                     /locus_tag="VYQ24_000360"
     CDS             718..1662
                     /locus_tag="VYQ24_000360"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1967545.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWINHAVYQETPFDWRKIEDDASAYGYL"
     gene            1671..2348
                     /locus_tag="VYQ24_000361"
     CDS             1671..2348
                     /locus_tag="VYQ24_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1967546.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            2447..3205
                     /locus_tag="VYQ24_000362"
     CDS             2447..3205
                     /locus_tag="VYQ24_000362"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1967547.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            3243..4748
                     /gene="lytC"
                     /locus_tag="VYQ24_000363"
     CDS             3243..4748
                     /gene="lytC"
                     /locus_tag="VYQ24_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1967548.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            5244..5762
                     /locus_tag="VYQ24_000364"
     CDS             5244..5762
                     /locus_tag="VYQ24_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1967549.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            5892..6398
                     /locus_tag="VYQ24_000365"
     CDS             5892..6398
                     /locus_tag="VYQ24_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1967550.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            6398..6568
                     /locus_tag="VYQ24_000366"
     CDS             6398..6568
                     /locus_tag="VYQ24_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967551.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            6600..7832
                     /gene="clpX"
                     /locus_tag="VYQ24_000367"
     CDS             6600..7832
                     /gene="clpX"
                     /locus_tag="VYQ24_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1967552.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKNENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            7841..8428
                     /locus_tag="VYQ24_000368"
     CDS             7841..8428
                     /locus_tag="VYQ24_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1967553.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            8439..8819
                     /locus_tag="VYQ24_000369"
     CDS             8439..8819
                     /locus_tag="VYQ24_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1967554.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            8871..9761
                     /gene="rapZ"
                     /locus_tag="VYQ24_000370"
     CDS             8871..9761
                     /gene="rapZ"
                     /locus_tag="VYQ24_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1967555.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            9758..10735
                     /locus_tag="VYQ24_000371"
     CDS             9758..10735
                     /locus_tag="VYQ24_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1967556.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            10732..11643
                     /gene="whiA"
                     /locus_tag="VYQ24_000372"
     CDS             10732..11643
                     /gene="whiA"
                     /locus_tag="VYQ24_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1967557.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHLYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(11677..12645)
                     /locus_tag="VYQ24_000373"
     CDS             complement(11677..12645)
                     /locus_tag="VYQ24_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1967558.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            12781..13638
                     /locus_tag="VYQ24_000374"
     CDS             12781..13638
                     /locus_tag="VYQ24_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1967559.1"
                     /translation="MNFQQLSNLQYWTSLFSSPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYTSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            13625..14404
                     /locus_tag="VYQ24_000375"
     CDS             13625..14404
                     /locus_tag="VYQ24_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1967560.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            14428..15780
                     /locus_tag="VYQ24_000376"
     CDS             14428..15780
                     /locus_tag="VYQ24_000376"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1967561.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            15854..16228
                     /locus_tag="VYQ24_000377"
     CDS             15854..16228
                     /locus_tag="VYQ24_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1967562.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            16230..17078
                     /locus_tag="VYQ24_000378"
     CDS             16230..17078
                     /locus_tag="VYQ24_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1967563.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            17243..18010
                     /locus_tag="VYQ24_000379"
     CDS             17243..18010
                     /locus_tag="VYQ24_000379"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1967564.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            18022..19206
                     /locus_tag="VYQ24_000380"
     CDS             18022..19206
                     /locus_tag="VYQ24_000380"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1967565.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            19203..21074
                     /locus_tag="VYQ24_000381"
     CDS             19203..21074
                     /locus_tag="VYQ24_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1967566.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKSDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLNKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMSRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(21217..22401)
                     /gene="mntE"
                     /locus_tag="VYQ24_000382"
     CDS             complement(21217..22401)
                     /gene="mntE"
                     /locus_tag="VYQ24_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1967567.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPILEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            22705..25401
                     /locus_tag="VYQ24_000383"
     CDS             22705..25401
                     /locus_tag="VYQ24_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1967568.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAMDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFASDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            25414..26205
                     /gene="yghU"
                     /locus_tag="VYQ24_000384"
     CDS             25414..26205
                     /gene="yghU"
                     /locus_tag="VYQ24_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1967569.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKVANRPAVKRGLEVTYTEIK"
     gene            26321..26731
                     /locus_tag="VYQ24_000385"
     CDS             26321..26731
                     /locus_tag="VYQ24_000385"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1967570.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVDVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            26743..28338
                     /locus_tag="VYQ24_000386"
     CDS             26743..28338
                     /locus_tag="VYQ24_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967571.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT         8266 a         5369 c         6636 g         8128 t
ORIGIN      
        1 aaaactttga aagaaagaaa aagatgaatt taaaagatta cattgcaaca attgaaaatt
       61 atccaaagga aggcattacc ttccgtgata ttagtccttt gatggctgat ggaaatgctt
      121 atagctacgc tgttcgtgaa atcgttcagt atgctactga caagaaagtc gacatgatcg
      181 tgggacctga agctcgtgga tttatcgtgg gttgtccagt tgcctttgag ttgggaattg
      241 gttttgcgcc tgttcgtaag ccaggtaaat tgccacgcga agttatttct gctgactatg
      301 aaaaagagta cggtgtcgat accttgacta tgcacgcgga tgccattaag ccaggtcaac
      361 gtgttcttat tgtagatgac cttttggcga caggtggaac tgttaaggca actatcgaga
      421 tgattgaaaa acttggtggt gttatggcag gttgtgcctt ccttgttgaa ttggatgaat
      481 tgaacggccg tgaaaaaatt ggtgactacg actacaaagt tcttatgcat tattaatgaa
      541 aacagtccct agggctgttt tctctacact aggatataaa aatagactat aactagttag
      601 agaaaaacta taattgaaaa ctatatcttc ttacagtata ataaaaggac taagtgtttg
      661 agatttgtct tcaaacatat gcaattattc ctgaaagagt acagttagga gagggttatg
      721 ccgattcgaa ttgataaaaa attgccagct gttgagattt tacggacaga gaatatcttt
      781 gtcatggatg atcaacgtgc tgcccaccaa gatatccgtc ctttgaagat tttaatttta
      841 aatctcatgc cacagaaaat ggtcacagag acccagttgt tgcgccactt ggctaataca
      901 cccctacaac tggatattga ttttctctat atggagagcc accgttctaa aacaactcgt
      961 tcagagcaca tggagacctt ctataaaact tttcctgaag tcaaggatga gtattttgat
     1021 gggatgatca tcacgggtgc tccagttgag catttaccat ttgaggaagt ggactattgg
     1081 gaggaattta gacagatgct tgagtggtct aagactcatg tctattcgac ccttcatatc
     1141 tgttgggggg ctcaggctgg gctttatctg cgctatggtg tagaaaaata ccagatggac
     1201 agtaagctat caggtattta tcctcaggac accctaaaag agggtcacct tctatttaga
     1261 ggctttgatg atagctatgt atcccctcat tcacggcaca cggagatttc taaggaagag
     1321 gtcttaaaca agaccaatct cgagatttta tcagaaggac ctcaggttgg ggtttctatt
     1381 ttggccagtc gtgatttacg agaaatttat agttttggtc atttggagta tgaccgtgat
     1441 accttggcaa aagagtattt tcgagatcgt gatgcaggtt tcgacccaca tattccagaa
     1501 aattacttta aagatgatga tgttaatcag gtaccttgtc tttgttggtc ttcatctgca
     1561 gccctctttt tcagtaattg gataaaccat gcggtctatc aggagacgcc ttttgattgg
     1621 agaaagatag aagatgatgc atctgcatat gggtatttat aagaggaatt atgacatatt
     1681 tagacgcttt taaatcaggt atcttggttt taccgagtgc cctgctcttg cattttaagg
     1741 aactctttcc ttctagcgac gattttctgg tttggcaatt tttctatttg caaaatacga
     1801 caggcttaga agaaatgtcg ccaagccaga ttgctgaaag gattggcaag gaaatttcgg
     1861 atgtcaacca gtccatttct aatctgacgg aaaggggact gctccagtat cgtactatcg
     1921 aattaaatgg cgaaattgaa ttgctctttg atgctagttt ggccttggaa cgtttggatg
     1981 acctgtttgg agcagttcat tcaagttcag accagctgac acctcaaaac cagctcaagg
     2041 atttggtgga aaccttccag caggagttgg gacgattgtt gacgcctttt gagattgagg
     2101 atttgaccaa gacactaaag gaagatggaa ccagtgctga cttgattaag gaggctcttc
     2161 gtgaagctgt tttgaatgga aaaccaaact ggaagtacat tcaggcgatt ttgagaaact
     2221 ggcgccatga aggaatcaag agtgtggctc aaattgaggc caagagagca gaaagagaag
     2281 caagcaatcc tcagttgaca caggtatctg cagatttcag aaatgccatg gatctctgga
     2341 aggattaatc catgcaagca ggcttgaaat ccgagtaaga tttgcaagct gtgtataatt
     2401 gtgatagaat aaaatagaaa ataaattgaa aaaagaggta tgtgaaatgt cacgtaaacc
     2461 atttatcgct ggtaactgga aaatgaacaa aaatccagaa gaagctaaag cattcgttga
     2521 agcagttgca tcaaaacttc cttcatcaga tcttgttgaa gcaggtatcg ctgctccagc
     2581 tcttgatttg acaactgttc ttgctgttgc aaaaggctca aaccttaaag ttgctgctca
     2641 aaactgctac tttgaaaatg caggtgcttt cactggtgaa actagcccac aagttttgaa
     2701 agaaatcggt actgactacg ttgttatcgg tcactcagaa cgccgtgact acttccatga
     2761 aactgatgaa gatatcaaca aaaaagcaaa agcaatcttt gcgaacggta tgcttccaat
     2821 catctgttgt ggtgaatcac ttgaaactta cgaagctggt aaagctgctg aattcgtagg
     2881 tgctcaagta tctgctgcat tggctggatt gactgctgaa caagttgctg cctcggttat
     2941 cgcttatgag ccaatctggg ctatcggtac tggtaaatca gcttcacaag acgatgcaca
     3001 aaaaatgtgt aaagttgttc gtgacgttgt agctgctgac tttggtcaag aagtcgcaga
     3061 caaagttcgt gttcaatacg gtggttctgt taaacctgaa aatgttgctt catacatggc
     3121 ttgcccagac gttgacggtg cccttgtagg tggtgcgtca cttgaagctg aaagcttctt
     3181 ggctttgctt gactttgtaa aataatcagt aagtagcaaa agctaggtgg aacagcattc
     3241 agatgtctgt tacatttttt ataggagagg aaagattgaa aataaaaatt ggattagcaa
     3301 gtatctgttt actaggcttg gcaactagtc atgtcgctgc aaatgaaact gaagtagcaa
     3361 aaacttcgca ggatacaacg acagcttcaa gtagttcaga gcaaaatcag tcttctaata
     3421 aaacgcaaac gagcacagaa gtacagacta atgctgctgc ccactgggat ggggattatt
     3481 atgtaaagga tgatggctct aaagctcaaa gtgaatggat ttttgacaac tactataagg
     3541 cctggtttta tattaattca gatggtcgtt actcgcagaa tgaatggcat ggaaattact
     3601 acctgaaatc aggtggatat atggcccaaa acgagtggat ctatgacagt aattacaaga
     3661 gttggtttta tctcaagtca gatggggctt atgctcatca agaatggcaa ttgattggaa
     3721 ataagtggta ctacttcaag aagtggggtt acatggctaa aagccaatgg caaggaagtt
     3781 atttcttgaa tggtcaagga gctatgatgc aaaatgaatg gctctatgat ccagcctatt
     3841 ctgcttattt ttatctaaaa tccgatggaa cttatgctaa ccaagagtgg caaaaagtgg
     3901 gcggcaaatg gtactatttc aagaagtggg gctatatggc tcggaatgag tggcaaggca
     3961 actactattt gactggaagt ggtgctatgg cgactgacga agtgattatg gatggtgctc
     4021 gctatatctt tgcggcctct ggtgagctca aagaaaagaa agatttgaat gtcggctggg
     4081 ttcacagaga tggtaaacgc tatttcttta ataatagaga agaacaagtg ggaaccgaac
     4141 atgctaagaa agtcattgat attagtgagc acaatggtcg tatcaatgat tggaaaaagg
     4201 ttattgatga gaacgaagtg gatggtgtca ttgttcgtct aggttatagc ggtaaagaag
     4261 acaaggaatt ggcgcataac attaaggagt taaaccgtct gggaattcct tatggtgtct
     4321 atctctatac ctatgctgaa aatgagaccg atgctgagag tgacgctaaa cagaccattg
     4381 aacttataaa gaaatacaat atgaacctgt cttaccctat ctattatgat gttgagaatt
     4441 gggaatatgt aaataagagc aagagagctc caagtgatac aggcacttgg gttaaaatca
     4501 tcaacaagta catggacacg atgaagcagg cgggttatca aaatgtgtat gtctatagct
     4561 atcgtagttt attacagacg cgtttaaaac acccagatat tttaaaacat gtaaactggg
     4621 tagcggccta tacgaatgct ttagaatggg aaaaccctta ttattcagga gaaaaaggtt
     4681 ggcaatatac ctcttctgaa tacatgaaag gaatccaagg gcgcgtagat gtcagcgttt
     4741 ggtattaagc gatgatttga aagagggatg tgatagtagc accctctttt tctttgtttt
     4801 atgatagttc atcctcgagt aaattcaaga tcttgctcgg aaatgaagct tatatagtag
     4861 attgaatata gacaaatacc ttgtgattgg taaaacattt tagaaattca tttacctttc
     4921 ctaatcgact tggtttcatc ttatttcaat ctattatagt attggggaat ttcttcaaac
     4981 cacatcagct tggtcagttc tacctgcgac ctcaaaactt gtgctttggt caagctgggt
     5041 ttagtttcct agtttgctga tggatttcca ttgactataa gcatccaacc ctctttttgt
     5101 cttctaaaga attattaaat tatcagtcta ttgcaacttt tctcatataa gttctttgtc
     5161 ttgctattgg ttttccttag tagtatacta aggtagtaat cattaagaag tggttacaaa
     5221 aaataatgaa tgaggtaaag aaaatggtag aattgaaaaa agaagcagta aaagacgtaa
     5281 catcattgac aaaagcagcg ccagtagcat tggcaaaaac aaaggaagtc ttgaaccaag
     5341 ctgttgctga tttgtatgta gctcacgttg ctttgcacca agtgcactgg tatatgcatg
     5401 gtcgtggttt ccttgtatgg catccaaaaa tggatgagta catggaagct cttgacggtc
     5461 aattggatga aatcagcgaa cgcttgatta cactcggtgg aagcccattc tctacattga
     5521 cagagttcct tcaaaatagt gaaatcgaag aagaagctgg tgaataccgt aatgttgaag
     5581 aaagcttgga acgtgttctt gttatctacc gttacttgtc agaacttttc caaaaaggtt
     5641 tggatgtcac tgatgaagaa ggtgacgatg tgacaaacgg tatctttgca ggcgctaaaa
     5701 ctgaaacaga taaaacaatt tggatgcttg cagccgaact tggacaagca cctggtttgt
     5761 aagaaataag gaaacataga taaatctgta ggaaatttct tacagatttt ttctattttg
     5821 taagctattc caaaccagtc tttttttgag ttttttgata aaatagtact atcagtgaaa
     5881 aggatggaag catgactaag aaaatcgtag ctatttgggc ccaggatgaa gagggtgtga
     5941 ttggtaagga aaatcgtctg ccttggcatt tgccagcaga attgcagcac tttaaagaaa
     6001 caactctgaa tcatgctatc ttgatggggc gtgtgacctt tgatgggatg gggcgtcgct
     6061 tgcttccaaa acgggaaacc ctgattttga cgcgtaatcc ggaagaaaag atagatgggg
     6121 ttgctacttt tcaggacgtc cagtctgttc ttgactggta tcaggatcaa gaaaagaatc
     6181 tctacattat cggtgggaag caaatttttc aggcttttga accttacctt gatgaagtga
     6241 ttgtcactca cattcatgct cgggtggaag gagataccta tttccctgaa gagcttgact
     6301 tgtctctttt tgagacagtt tcaagcaaat tttacgccaa agatgagaag aatccttatg
     6361 attttaccat ccaataccgc aagagaaagg aagtataatg gaacggagtt tatttggtct
     6421 attcacggct tttctgtgtt tcatctgctt tctggcagga gcacaggctt ttcgtaaaaa
     6481 gcgttacggg ctttctatcc tcctttggtt aaatgccttt accaatctgg tcaatagtat
     6541 tcatgctttt tacatgacct tattttagat agaatgacag tatagaatgg aaggaaatca
     6601 tgtctacaaa tagaaaaaat gatatgatgg tttattgctc attttgtggc aaaaaccaag
     6661 aagaagtaca aaaaataatt gctggcaaca atgcttttat ttgtaatgaa tgcgtggagt
     6721 tagctcagga aatcattcga gaagaattgg ttgaggaagt cttggcagac ttgtctgagg
     6781 tgccaaaacc aattgaactc ctccatatct tgaaccacta tgtaattggt caagatcgtg
     6841 ccaagcgtgc cttggcagtg gcggtttata accactacaa acgcatcaat ttccacgata
     6901 cacgcgaaga gtcagaagat gtggatttgc agaagtcaaa cattttgatg attggcccaa
     6961 ctggttcagg gaaaactttc cttgcccaga ccttggctaa gagcttgaat gtaccttttg
     7021 ctattgcgga tgcgacagct ctgacggagg ctggttatgt gggtgaggat gtggaaaata
     7081 tactcctcaa actcttgcag gttgctgact ttaacatcga acgtgcagag cgtggcatta
     7141 tctatgtgga tgaaattgac aagattgcca agaagaatga gaatgtgtct atcacacgtg
     7201 atgtttctgg tgaaggggtg caacaagccc ttctcaagat tatcgaggga actgttgcta
     7261 gcgtaccgcc tcaaggtgga cgcaaacatc cacaacaaga gatgattcaa gtggatacaa
     7321 aaaatatcct cttcatcgtg ggtggtgctt ttgatggtat tgaagaaatt gtcaaacaac
     7381 gtctgggtga aaaagtcatc ggatttggtc aaaacaataa ggcgattgac gaaaacagct
     7441 catacatgca agaaatcatc gctgaagaca ttcaaaaatt tggtattatc cctgagttga
     7501 ttggacgctt gcctgttttt gcggctcttg agcaattgac cgttgatgac ttggttcgca
     7561 tcttgaaaga gccaagaaat gccttggtga aacaatacca aaccttgctt tcttatgatg
     7621 atgttgagtt ggaatttgac gacgaagccc ttcaagagat tgctaataaa gcaatcgaac
     7681 ggaagacagg ggcgcgtgga cttcgctcca tcatcgaaga aaccatgcta gatgttatgt
     7741 ttgaggtgcc gagtcaggaa aatgtgaaat tggttcgcat cactaaagaa actgtcgatg
     7801 gaacggataa accgatccta gaaacagcct agaggtgact atggaactta atacacacaa
     7861 tgctgaaatc ttgctcagtg cagctaataa gtcccactat ccgcaggatg aactgccaga
     7921 gattgcccta gcagggcgtt caaatgttgg taaatccagc tttatcaaca ctatgttgaa
     7981 ccgtaagaat ctcgctcgta catcaggaaa acctggtaaa acccagctcc tgaacttttt
     8041 taacattgat gacaagatgc gctttgtgga tgtgcctggt tatggctatg ctcgtgtttc
     8101 taaaaaggaa cgtgaaaagt gggggtgcat gattgaggag tacttaacga ctcgggaaaa
     8161 tctccgtgcg gttgtcagtc tagttgacct tcgtcatgac ccgtcagcag atgatgtgca
     8221 gatgtacgaa tttctcaagt attatgagat tccagtcatc attgtggcga ccaaggcgga
     8281 caagattcct cgtggtaaat ggaacaagca tgaatcagca atcaaaaaga aattaaactt
     8341 tgacccaagt gacgatttca tcctcttttc atctgtcagc aaggcaggga tggatgaggc
     8401 ttgggatgca atcttagaaa aattgtgagg aaaagaaaat ggcaaaaaca attcatacag
     8461 ataaggcccc aaaggctatc gggccctatg ttcaaggaaa aatcgttggc aaccttttgt
     8521 ttgctagcgg tcaagttccc ctatcccctg aaactgggga aattgtagga gagaatatcc
     8581 aagaacagac agagcaagtc ttgaaaaaca tcggtgctat tttggcagaa gcaggaacag
     8641 actttgacca tgttgtcaaa acaacttgtt tcttgagcga tatgaacgac tttgttcctt
     8701 ttaatgaggt ttaccaaacg gccttcaaag aggaattccc agctcgttca gctgtggagg
     8761 tagctcgtct tcctcgtgat gtaaaagtcg aaattgaagt catcgcagag attggataag
     8821 ctagttgaag tttggtgttg ccaaacttct tttgatataa ggagaaaaag atgacaaaga
     8881 aacaacttca cttggtgatt gtgacaggga tgagtggcgc agggaaaact gtagccattc
     8941 agtccttcga ggatctaggt tatttcacca ttgataatat gccgccagct ctcttgccta
     9001 agtttttgca gctggttgaa attaaggaag acaatcctaa gttggccttg gtagtggata
     9061 tgcgtagccg ttctttcttt tcagagattc aagctgtttt ggatgagttg gaaaatcaag
     9121 atggtttgga tttcaaaatc ctctttttgg atgcggctga taaggaattg gtcgctcgtt
     9181 acaaggagac cagacggagt cacccactag cagcagacgg tcgtatttta gatggaatca
     9241 agttggaacg tgaactcttg gcacctttga aaaatatgag ccaaaatgtg gtggatacga
     9301 ctgaactcac tccacgtgag ctgcgcaaaa cccttgcaga gcagttttca gaccaagaac
     9361 aagcccagtc tttccgtatc gaagtcatgt ctttcggatt taagtatgga atcccgattg
     9421 atgcggactt ggtctttgat gtccgtttct tgccaaatcc ctattatcta ccagaactga
     9481 gaaaccaaac gggtgtggat gaacctgttt atgattatgt catgaaccat cctgagtcag
     9541 aagactttta tcaacattta ttggccttga ttgagccgat tctgccaagt taccaaaagg
     9601 aaggtaagtc cgttttgacc attgccatgg gatgtacggg tggacaacac cgtagtgtgg
     9661 catttgctaa acgcttggcg caggacttat ccaagaattg gtctgttaat gaagggcatc
     9721 gcgacaaaga ccgcagaaag gaaacggtaa accgttcatg agaaaaccaa agataacggt
     9781 gattggtgga gggactggaa ttcccgtcat tctaaaaagt ctgcgggaaa aagatgtgga
     9841 aatcgcagct atcgtgacgg tggcagatga tggtggttct tcaggtgaac tccgaaaaaa
     9901 tatgcaacag ttgacaccgc caggtgatct tcgtaatgtc cttgtggcca tgtcggatat
     9961 gcctaagttt tatgagaagg tctttcagta tcggttctct gaggatgccg gagcctttgc
    10021 tggccatcca ttgggaaatc tcatcattgc tggcttgtca gaaatgcagg gttcaaccta
    10081 taatgccatg cagttattga gcaaattttt ccatacaaca gggaaaattt atccttccag
    10141 tgaccatcct ttgacccttc atgcagtctt tcaggatggg acagaagtgg ctggagagag
    10201 tcatattgta gaccatcgag gcataattga caatgtctat gtgaccaatg ccctaaacga
    10261 tgatacgcct ctggccagcc gtcgagtagt gcagaccatc cttgaaagtg acatgattgt
    10321 cctagggcca ggttccctct ttacctctat tttgcccaat atcgtgatta aggaaattgg
    10381 gcgggctctt ttggaaacca aggcagaaat tgcctatgtc tgcaatatca tgacccaacg
    10441 tggggagacg gaacacttta cagatagcga ccacgtggaa gtcttgcatc gtcaccttgg
    10501 tcgccctttt atcgacactg tcttggtgaa tattgaaaaa gtgcctcagg aatacatgaa
    10561 ttccaaccgt tttgatgaat acttagtgca agtggaacac gattttgtag gtctttgtaa
    10621 gcaagtttcg cgcgtgattt catctaactt ccttcgtctg gaaaatggcg gtgccttcca
    10681 cgatggagat ttgattgtgg acgagttgat gcgcattata caggtgaaaa aatgagtttc
    10741 acagtagcag taaaagaaga aatcctgggc caacaccatc tgagctggca tgaattatct
    10801 gccattatca aaatgtctgg tagcatcggt ctctcgactt cgggcttgac tttgtcagtt
    10861 gtgacagaaa atgccaaact ggcccgtcac ctctatgagt cctttctcca tctctatgaa
    10921 atcaaatctg aaattcgtca ccaccaacgg agcaatcttc gcaagaatcg cgtctatacc
    10981 gtttttacag atgaaaaggt gcaggatttg ttaagtgatt tgcacttggc agactctttc
    11041 tttggcctgg aaacaggtat tgatgaggcg attttatcgg atgaggaagc aggtcgtgcc
    11101 tatctctgtg gcgctttctt ggcaaatggg agcattcgtg accctgagtc aggcaagtac
    11161 cagttggaaa tcagttctgt ttatctggac cacgcgcaag gaattgcctc ccttctccaa
    11221 cagtttttac tggatgccaa ggtgcttgag cgcaagaagg gggctgtgac ctatctccag
    11281 cgagcagaag acattatgga cttcttgata gtaataggag ccatgcaggc acgtgatgat
    11341 tttgagcggg ttaagatttt gcgagaaacc cgtaacgacc tcaatcgggc taataatgcc
    11401 gagacagcta atatcgctcg gacagtttct gccagcatga agaccattaa caatatcagt
    11461 aaaatcaaag atatcatggg cttagaaaat ttgccagtag atttgcagga agtggcgcaa
    11521 ctgaggattc agcacccaga ctactctatc cagcagttag cagatagtct cagcacccct
    11581 ctgaccaaaa gtggtgtcaa tcacagactc agaaaaatca ataagatagc ggatgaatta
    11641 taaaaccacg aatcctatgt gactcgtggt tcttttttat aaactagtag agtgttttgg
    11701 ttgtactttt tgttcagggt caatgtagtt gatagcgttg ttgacagcag ttggagcttc
    11761 tccgaggcct gtcgcaatca gatcaatttt tccgtcatag tagcagcagt caccgatagc
    11821 atagatacct gcttggctgg attcctgttt gctgttgacg ataatcttgt gacggttgag
    11881 gtcgagcccc cagtttttaa ggttaccgac agaagatttg aaaccatagt tgacaaagag
    11941 gtggtctagg tcaatggttt cagtttcatc agatttgact tttgtgattt caagtttatc
    12001 aagtgttttt ccatttccaa ggagttggct aggggcgaat ggtgtcttga tggttacaga
    12061 tgattcttgc aaggcttgaa cactgtgttc caaggcacgg aaattatctc tgcggtgaac
    12121 aagggtagtt ggtgcgattt tttcaaaagc caaagcccaa tccacagccg agtctccccc
    12181 accaagaatc gtcactttct taccagcgta ttgctgaatg ttagaaacgt ggtagtggat
    12241 attttcatag ccctcaacac cttcaagttc cagcggacgt ggtttgaagg caccgccacc
    12301 catagcgatg atgactgttt tagtcaggtg acttccttta gaagttgtga tggcaaattc
    12361 ttcttcttgt ttgtcaatct caagaaccgt ttcattgaga tgaatagggg tatcaaatcc
    12421 atttagctgt tcaatcaagc ggttagtcaa ctcttctcca gtcaggtttg ggaagcctgg
    12481 tacgtctagg atttcctttt cagggtagag aatagcaggt tgtccaccta gctggggaag
    12541 agagtcgatg atttgaacct tggcttggcg taggtgggca taaaaggctg caaaaagccc
    12601 gacaggacca ccacccacaa tggtaatatc atagagttga gacatagttt ctcctttgtt
    12661 ttttctagtc agtttatttt atcatatttt tgttcaatgt aaaatggact aggataggga
    12721 aaaaaatgcc agaaaatggt ataataggac gaaagtgttt tgatagggag gatatcaggg
    12781 atgaattttc aacaattatc caatctgcaa tattggacca gtttgttttc aagtccatgg
    12841 acgatagcta tcaatctgat tgatattttg attgttgctt atattttata ccattttaca
    12901 aaagctattg caggaaccaa gattatgatt ttggtacgtg gagttttggt gtttatttta
    12961 gctcaaatcc ttgcaaatat gattggtttg actacgattt cttggttaat caatcaaatt
    13021 attacttatg gggttattgc ggcggttgtt atcttctctc cagagattcg gactggtttg
    13081 gaacgtttgg gaagagcgac agatttcttt tccaatgccc ctattagtgc tgaggaacag
    13141 atgattcgtg cctttgttaa gtctgttgaa tacatgagtc ctcgtaaaat cggggccttg
    13201 gttgctattc agcgtgtacg taccttgcag gagtatactt cgacaggaat tcccttggat
    13261 gctaagattt ctgcagaact tctcattaac atttttattc ccaacactcc cctacatgat
    13321 ggtgcggtga ttatcaaaga agaacgtatc gctgtgacgt ctgcctatct gcccttgaca
    13381 aaaaacacag ggatttccaa ggaatttggg accagacacc gggcggctat cggtttatca
    13441 gaagtctcag atgccttgac ttttgtcgta tcagaggaaa cgggaggaat ttcgataacc
    13501 tataatggaa ggtttaagca caacctaaca cttgatgaat ttgaaacaga attacgtgaa
    13561 atcttacttc caaaagagga agtgggtctt agttttaaag aacgattgct aggaggatgg
    13621 aaacatgaaa aaaaatagtt tatatatcat atcctcactc ttttttgctt gtgtcttatt
    13681 tgtctatgct acggcgacga attttcaaaa cagtaccagt gctaggcagg taaaaacgga
    13741 aacctatact aatacagtaa caaatgtccc tattgacata cgctataata gtgataagta
    13801 ttttattagc ggttttgctt cagaagtatc agtggtcttg actggtgcaa atcgcctatc
    13861 gctagctagt gaaatgcaag aaagtacacg taaattcaag gttactgctg acctaacaga
    13921 tgccggtgtt ggaacgattg aagttccttt gagcattgaa gatttaccca atgggctgac
    13981 cgctgtggcg actccgcaaa aaattacagt caagattggt aagaaggctc agaaggataa
    14041 ggtaaagatt gtaccagaga ttgaccctag tcaaattgat agtcgggtac aaattgaaaa
    14101 tgtcatggtg tcagataaag aagtgtcgat tacgagtgac caagagacat tggatagaat
    14161 tgataagatt atcgctgttt tgccaactag tgaacgtata acaggtaatt acagtggttc
    14221 agtacctttg caggcaatcg accgcaatgg tgttgtctta ccggcagtta tcactccgtt
    14281 tgatacaata atgaaggtga ctacaaaacc agtagcacca agttcaagca catcaaattc
    14341 aagtacaagc agttcatcgg agacatcttc gtcaacgaaa gcaactagtt caaaaacgaa
    14401 ttaaaaatag aaaaggattt tataacaatg ggtaaatatt ttgggactga tggagtccgt
    14461 ggagaagcta acctagaact aacaccagaa ttagccttta aactaggacg ttttggaggc
    14521 tatgttctta gtcaacatga aacggaagcg ccgaaagtct ttgtaggacg tgacacacgt
    14581 atttcagggg aaatgttgga atcggccttg gtggcaggtc tcctttcagt agggattcac
    14641 gtatacaaac ttggtgtcct tgcaacacca gcagtagctt acttggttga aactgaagga
    14701 gcaagtgccg gtgtcatgat ttctgctagc cacaacccag cccttgataa cggaatcaag
    14761 ttctttggcg gtgatggctt caaactagat gatgaaaaag aagcagaaat tgaagccttg
    14821 ctagatgctg aggaagacac tcttcctcgt ccaagtgcag aaggcttagg aattttggta
    14881 gattatccag aaggcttgcg taagtatgaa ggataccttg tttcaactgg aactcctctt
    14941 gatggaatga aggttgcctt ggatacagct aatggagcag cttctaccag tgcccgtcaa
    15001 atctttgcag accttggtgc ccaattgacg gttatcgggg aaacaccaga cggtcttaac
    15061 atcaacctta atgttggttc aacacatcca gaagcccttc aagaagtggt caaagaaagt
    15121 gggtcagcta ttggtttggc ctttgatgga gacagtgacc gcttgattgc tgttgatgag
    15181 aatggtgaca tcgttgatgg tgacaagatt atgtacatca tcggaaaata cctttctgaa
    15241 aaaggacaat tggctcaaaa tacaattgtg acaactgtta tgtctaacct tggtttccac
    15301 aaggccttga atcgcgaagg tattaacaag gcagttactg cagttggtga ccgctacgtt
    15361 gttgaagaaa tgagaaaatc aggctacaac cttggtggtg aacagtctgg tcacgttatc
    15421 ttgatggatt acaataccac aggtgatggt caattatcag cagttcaatt gactaaaatc
    15481 atgaaggaaa ctggtaagag cttatcagag ttggcggcag aagtaacgat ttatccacaa
    15541 aaattagtta atatccgagt ggaaaacgtc atgaaggaaa aggccatgga agtgccagct
    15601 atcaaggcca tcatcgagaa gatggaagaa gaaatggcgg ggaacggccg tatccttgtt
    15661 cgtccaagtg gaacagagcc cctcttgcgt gttatggcag aagcgcctac aacagaagaa
    15721 gtggactact atgttgatac catcacagat gtagttcgtg ctgaaattgg gattgattaa
    15781 atcctagaaa actagatgaa aataaaaaat tatggtacaa tagcttataa tattgtagcc
    15841 gatggagaaa gacatgaatc ttaaacgtga acaagaattt gttagtcagt atcattttga
    15901 tgcgcgtaat tttgaatggg aaaatgaaaa tggagctcct gaaaccaagg tggatgtgaa
    15961 tttccaattg ctccaacatg accaagaaaa tcaagtgact tccttgattg tcatcttgag
    16021 ctttatgatt gtatttgata agtttgttat tagtggaaca atctcacaag taaatcatat
    16081 cgatggccgt attgtcaatg aaccaagtga attgaaccaa gaagaggtgg aaaccttggc
    16141 tcgtccatgt ttgaacatgc tcaatcgttt gacttacgaa gtgactgaaa ttgcattaga
    16201 cttaccagga attaatttgg agttctaata tgaaattagc tgttttcaca gattcctctg
    16261 cttatctcag tgcagagacc ttgcaaagag aagacttgtt tgtcttggat attcctgtca
    16321 atattgatgg tgaggaatat gtcgaaggca tcaatctgtc tgctgaggaa ttttaccaaa
    16381 aaatggctca ggcttctgaa ttgcctaaga ccagtcaacc aagtattgcc aagttagatg
    16441 agatcttaac ttcgctcaaa gaacaaggct atacacatgc cttggggctt ttcctatctt
    16501 ctggaatttc aggtttttac caaaatatcc agtatatggt cgatgactat gagggcttaa
    16561 ccattgcttt cccagacact ttgattacaa gtgctcccct aggcatcatg gttgaaagcg
    16621 tctttaattg gcgtgatcag ggcgatgatt ttgccagcat tcaggataag ctagccattc
    16681 aaatcagccg tacgtcagcc tttatcatgg tagatgactt ggatcatttg gtaaaaggtg
    16741 gacgcctttc aaatggggct gccattttgg gcaatttgct tagcattaag ccaatccttt
    16801 attttaacga tcaaggtgtg attgaagttt acgaaaaagt tcgtactgaa aagaaggcca
    16861 ccaagcgctt aattgaaatt atcaaggaaa caacggcttc aggtcaatac cgggtcattg
    16921 tcattcatgg gaatgctcct gaaaaggctg aagaattgcg tcagcacttg cttgattttg
    16981 gcttgggttc ggatgtttca cttgctacat ttggtagtgt cattggaacg cacctaggag
    17041 caggaagtat tgctctgggt tatattccag tgatttagtt ggcgcttcta ggctagttaa
    17101 gatgccttgt atcttagaaa ttgaacacgc ctggaaccct gtgtgaaaaa gatagttctt
    17161 ccaagaagta gacacttctt gattggaatt cctattttca ctttgtgttc ttacggactt
    17221 tgtatcttag acaggagtag agatgagtat tcgagtaatt attgccggtt ttaagggaaa
    17281 gatgggccag gctgcttgtc agatggtatt gactgatcca gacttggact tggtggcagt
    17341 tttggatcct tttgagtctg agtcagaatg gcagggtatt cctgttttca aggataaggc
    17401 tgatttagct ggttttgaag cggatgtctg ggtagatttt actactccag ctgttgccta
    17461 cgaaaataca cgttttgctc ttgaaaatgg ctttgctcca gtagttggaa cgactggttt
    17521 cacgagtgaa gaaattgcag agctaaaaga attttctcgt gcccaagact tgggtggcct
    17581 gattgcccct aactttgcct tgggtgctgt cttactcatg caatttgcga cgcaggctgc
    17641 caaatatttc ccaaatgtgg agattattga gctccatcat gacaagaaaa aggatgctcc
    17701 gagtggaaca gccattaaaa cagctgagtt gatggcagag gttcgagagt ccattcagca
    17761 aggtgcagca gatgaggaag agctgattgc tggtgctcgt ggtgctgact ttgatggtat
    17821 gcgcatccac tcagttcgtt tgccaggctt ggtagctcat caggaagtca tctttggcaa
    17881 tcagggagaa gggttgaccc tccgtcatga ctcctatgat cgcatctcct tcatgacagg
    17941 agtcaatttg ggaattaaag aagttgtcaa gcgtcatgag cttgtctatg gattagaaca
    18001 cttattatga gattaacgca aatgccttct gaatttcaga aggctttacc agtattagaa
    18061 aaaattaaag aagcaggctt tgaggcttat tttgttgggg gctctgttcg agatgccctt
    18121 ctccatagcc ctatccacga tgtggatatt gcgacgtctt cttatccaga agagaccaag
    18181 cagatttttc cgcgaacagc cgatatcgga atcgagcatg gaaccgtctt ggtcttagat
    18241 ggggatgagg agtatgaggt aacaaccttt cggacagagg atgtctatgt ggactatcgc
    18301 agacccagtg cggtttcctt tgtgcgctcg ctagaagaag acctcaaacg ccgtgatttc
    18361 acagtcaacg cctttgcctt ggatgagaca ggagaaatcg ttgacttgtt ccatggttta
    18421 gaagatttgg aaaagcaagt cttgcgagca gttggagtgg ctagtgagcg tttcaacgaa
    18481 gatgctttac ggattatgcg tggtttccgt tttcaggcta gtcttggttt tgcacttgag
    18541 ccagaaacat ttaaagctat gaagaccttg acgccgcttt tggagaaaat ttctgtagag
    18601 cgtaccttcg tcgaatttga taaactcttg ctggctccat tttggagaag gggcttggct
    18661 tccatgattg agagtcaagc ttatgactat ctccctgata tggcatctag ccaggacaag
    18721 ctcaacagac tgtttgattt ggagactgat tttacttttg aatcctctga acaagcctgg
    18781 gcggctttac tatgggcttt ggagattgaa aatgcgcagt catttttgaa atcttggaag
    18841 acctcacgcc agtttgccaa gcaagttcag gatttgctga ttattttggc tctgcgtgaa
    18901 aatggagaat tgagcaagcg agattgttat cgctttgaca tagatttgct tttacaggct
    18961 gaaaatcttc gtcaagctca aggaaaagaa gtcaacccac aagccatcac agaaaaatac
    19021 caaagcttga ctatccatga caagaaagag attcagatta atggcgatat tttgatcaag
    19081 gaatatggct atcagccagg cccagacttg ggagagattt taacagagat tgagtttgcc
    19141 attgtcgatg gagaattgga gaataatcgt gaagccatcc atgcttacct gagggagaaa
    19201 aaatgagtga ttttatcgtt gaaaaactaa gtaaatccgt tggtgacaag accgttttta
    19261 gggatatttc ctttattatc catgacttag acagaattgg tttaatcggt gtcaatggga
    19321 ctggcaagac cacccttttg gacgtccttt ctggtgtttc tggatttgat ggggatgtca
    19381 gtcctttttc agctaaaagt gattaccaga ttggttattt gactcaggat cctgattttg
    19441 atgatagaaa gacagttttg gatacggttc tatctagtga actcaaggaa atccagctca
    19501 ttcgtgagta tgaattgatt atgctcgact atagtgagga caagcaggcg cgtttggaac
    19561 gtgtcatggc agagatggac tctctccaag cttgggaaat cgagagtcag gtcaagaccg
    19621 ttcttaacaa attgggcatt caagacttat ctactcctgt tggggaattg tcaggtggtc
    19681 tgagaagacg ggtacagttg gcacaagtct tacttggcaa ccacgacctt ttgcttttgg
    19741 atgagccgac caaccatctg gatattgcga ttattgagtg gctgaccctc tttttgaaaa
    19801 attctaagaa gaccgtcctt tttatcactc acgatcgtta tttcttagac gctttgtcaa
    19861 cacggatttt cgagttggag cgagcaggct tgacagaata tcagggcaat taccaggact
    19921 atgttcgcct taaggcagaa caggacgagc gcgatgcagc tctacttcat aaaaaagaac
    19981 aactctacaa acaagaactg gcctggatga gcagacaacc gcaggcgcgt gcgaccaagc
    20041 aacaagctcg tatcaatcgt ttccatgatc tgaaaaagga agtttcaggc agtagtgctg
    20101 agacagactt gactatgaac tttgaaacca gtcggattgg gaagaaagtc atcgagtttc
    20161 aggatgtttc ctttgcctat gaaaataagc ccattttgca agattttaat ctcttagttc
    20221 aggctaaaga ccgtattgga attgttgggg acaatggtgt tggaaaatca accctactta
    20281 acctgattgc aggaagtctt gagccgacag caggacaagt tgtgattggg gaaactgttc
    20341 gcatcgccta tttctctcaa caaattgagg gtttggatga aagcaagcgt gtgatcaatt
    20401 acctgcagga agtggcagag gaggtcaaga ccagtggtgg ttctacgact tccatcgctg
    20461 agttgctgga gcaattcctc ttcccacgtt cgacgcatgg gaccttgatt gagaagttgt
    20521 cagggggtga gaaaaaacgt ctttatctcc tcaaactgct tttggaaaaa ccaaatgttc
    20581 ttcttttaga cgagccaacc aatgacctag atattgcaac tttgacagtc ttagagaatt
    20641 tcttgcaagg ttttgcaggt cccgttttaa cagtcagtca cgaccgctat ttcttggata
    20701 aggtagcgac caagattctc gcttttgagg atggcaagat tcgtcctttc tttggtcatt
    20761 acaccgacta tcttgatgaa aaagcttttg aaacagatat ggccaatcaa gtgcaaaagg
    20821 ccgaaaagga aaaagtggtc aaggttcgag aagacaagaa acgcatgacc taccaagaaa
    20881 agcaggagtg ggcaagtatt gaaggtgata ttgaaacctt ggaaaaacgt atcgctgcta
    20941 ttgaagagga aatgcaggct aacggctctg actttggtaa gctggctact ctccaaaaag
    21001 aattggatga gaaaaatgaa gcactccttg aaaaatacga acgctatgag tatctcagtg
    21061 aatttgatag ttaagactat tcgaaaatca aagtgcaaac tatgtcggct tcacattgcc
    21121 gactcaagta cagcctgcga ccggcttcct aatttgcact ttgattttta ttgagtataa
    21181 acgaatagaa aaatctcgtc tcatgaaagg atttctctat tcttttttgg tttcttgatg
    21241 aaagatattt tgccaggttt cgtggcgacg ccagatactg gtatggatga taccatctaa
    21301 ctcatagcag atgagtttgg ttttttgact gatggaagta atttgaatgt ccttgatagc
    21361 agaatttaac tcttttttgg tcttataagc ctctttatcc atctgctctc catcttggcg
    21421 aatatagaca aaatcatcag tcaagagttc ttctagttgg tttccttggt caatcaattg
    21481 ttcacgcata agcaattttt tatagacatt gtctaaaatt tcatcctcaa ggataggtgc
    21541 tggttcgata tggacatcgg tatcaaagac gccaaaacgc tcctccagca tagactcgac
    21601 ctgatccgcg atttcatggc tttcaaaaac agacaagtca ggattcatct ctagtgtaat
    21661 atccaggtag atgttgctac cgtaggtgcg acctctttgc gatttgacct tgctgatttt
    21721 gggaatttcc atgatagcct tttggtagtc ctcgagcagg cggtcgtcaa agccatctga
    21781 aagactaaag gaagactcga tgaagatatc ataggcagtc ttcaagataa agaaagtgat
    21841 gatgatagca accagtttat ccacaatcgg ataattgaaa ctactagcta ggatggcaat
    21901 ggcggttcca agtgaggtaa cagcgtcaga aagattgtcc ttagcagctg ccttcagcgc
    21961 cttggagttg gatttcttac tgaggcgagt attgtagaga tagaccacaa acataatcgc
    22021 tgcagaaatg attcctagag ttgcaccaag aggatcaatg accgtttctt cccgactgag
    22081 aatcttttga atggtatctc ttagaacatc gaaaccgaca tagaacatga tgatagaagt
    22141 gatcaagctt gccaaatctt caatcttcca atgaccaaaa cggtggtcac ggtctgcagg
    22201 ctggcgcgcc atccgaatcc cgattaagag ggctacattt ccaatgatgt ccgatacgtt
    22261 attaaaacca tcggccacca aactggatga atgaaggaga tgaccagctg ctaatttggc
    22321 tgcagacaag atcaaatagg tcgaaatact gataatggct ccacgctcag ctaacttgag
    22381 atttgagata gattgcttca ttcaacttcc tttctagtca catagcattc taccattata
    22441 ctggttttca aggaaaaatt caaataaggc agtttttact ctttgaaaac tccccaaaat
    22501 ttggtataat ggtactactc aagggagtag ctggcagaaa cctgtgatag tgtcgtcatt
    22561 ccgaatttta tgctgaaaag tatgctttcc ggccctatct taaacagcga gacttgttat
    22621 gattaacagg tctctttttg tttgtcgtga aaagatatga cagaaagaga gtctgttttg
    22681 cacacaatgt caaggaggag acacatgtca aaagaacaaa aacgccaagc gttttatact
    22741 cagagccctg aagaggtctt gcaggctatg gatgcgaccg agcaaggttt gtcatcaagt
    22801 gaggcggaaa agcgccttgc cgaatttggg cacaatgaac tcgaagaagg cgagaaacga
    22861 tcaatcttgg tcaaattcat cgagcaattt aaggatttga tgattatcat cctagttgcg
    22921 gcagcaatct tgtcagtcgt gacttctggt ggggaagata tcgcagatgc cattatcatc
    22981 ctagctgtgg ttatcatcaa cgctgccttt ggtgtttacc aagaaggaaa agctgaagaa
    23041 gctattgaag ccctcaaatc catgtctagt ccagttgccc gcgttcttcg tgatggacac
    23101 atggcagaga ttgactctaa agaattggta ccaggcgata tcgttgccct tgaagcaggt
    23161 gacgtggtac cagcggacct acgtttgata gaagccaact ctcttaaaat tgaagaagca
    23221 gccttgacag gtgaatctgt accagtcgaa aaagacttgt cagtcgagct tgcgacagat
    23281 gctggtattg gtgaccgtgt caacatggcc ttccaaaact caaacgtaac ctatggtcgt
    23341 gggatgggtg ttgttgtcaa tacaggtatg tatactgaag ttggtcatat tgctggtatg
    23401 cttcaagatg cggatgagac tgatacacca ctcaaacaaa acttgaacaa cctttctaag
    23461 gtcttgacct atgctatctt ggtcattgcc cttgttactt ttgtagtggg tgtcttcatt
    23521 caagggaaaa atccacttgg tgagttgttg acttctgttg cccttgccgt tgcagccatt
    23581 ccagaaggac ttcctgctat cgttaccatc gttctttctc ttggtactca agttttggcc
    23641 aaacgacatt ccatcgttcg taagttgcca gcagttgaaa cacttggttc aactgaaatc
    23701 atcgcttctg ataagactgg tacgctgact atgaacaaga tgacagtcga aaaagtcttc
    23761 tacgatgcgg ttctacatga ctcagctgat gatattgaac taggtcttga aatgccacta
    23821 cttcgttcag ttgtcttggc caatgatacg aaaatcgatg tggaaggtaa cttgattggt
    23881 gacccaaccg aaacagcctt tatccaatat gccttggaca agggctatga tgtcaaaggt
    23941 ttcttagaga aatatcctcg tgtagctgaa ttgccatttg cctctgaccg taagctcatg
    24001 tcaacagttc acccattgcc agatggtcgt ttccttgtag cagtcaaggg tgcgccagac
    24061 caactcttaa aacgttgtct tcttcgtgat aaggctgggg atattgctcc gattgatgag
    24121 aaggttacaa atctcattca tacaaacaat tctgaaatgg ctcatcaagc cttgcgtgtc
    24181 cttgcaggtg cttataagat tatcgatagt attccagaaa atctcacttc tgaagagctt
    24241 gaaaatgatt taatttttac tggtttgatt gggatgattg accctgaacg tcctgaagcc
    24301 gctgaggctg ttcgtgtggc taaggaagcg ggaatccgtc cgattatgat tacaggtgac
    24361 caccaagaca cagcggaagc cattgccaaa cgtttgggaa tcattgacgc aaacgataca
    24421 gaaggtcacg ttttaactgg tgctgaactc aatgaactgt cagatgaaga atttgaaaaa
    24481 gtcgttggtc aatactctgt ttatgcccgt gtgtctccag aacacaaggt tcgtatcgtc
    24541 aaggcttggc aaaaacaagg taaagtcgtt gccatgacag gtgacggtgt caatgacgcg
    24601 ccagctctga aaacagccga tatcggtatc ggtatgggaa tcactggtac agaggtttct
    24661 aagggggctt ctgatatgat tcttgcagat gataactttg cgactattat cgtcgcagtg
    24721 gaagaaggac gtaaggtctt ctcaaacatt caaaagacta ttcagtacct actttctgct
    24781 aatactgctg aagtattaac catcttccta tcaaccttgt ttggttggga tgtcttgcag
    24841 ccggttcatc ttttgtggat caacttggta acggatacct tcccagctat cgctcttggt
    24901 gttgaacctg cggagcctgg tgtcatgaat cataaaccac gtggacgcaa ggcaagcttc
    24961 ttctcaggtg gtgttttgag ttctatcatt tatcaaggtg tactccaagc agctcttgtt
    25021 atgagtgttt atggccttgc gattgcttac ccagttcatg tgggtgacaa tcatgctatt
    25081 catgcagatg ccctaacgat ggcctttgca acccttggtt tgattcagct cttccatgcc
    25141 tacaatgtca agtctgtcta ccaatccatc ttgacagttg gcccattcaa gtctaagacc
    25201 tttaactggt ccatcttggt atcctttatc cttctcatgg caacaatcgt tgtagaaccg
    25261 cttgaaggaa tcttccacgt aaccaaacta gacttgtcac aatggggaat tgttatggct
    25321 ggaagcttct caatgattat catcgtcgaa atcgttaagt ttattcaacg caaactcggt
    25381 tttgacaaga atgcgattta agggaggttt cggatgtcag catatcaatt accgaccgta
    25441 tggcaggatg aagctagtaa tcaaggagct tttacggggc taaacagacc aacagcaggt
    25501 gcccgtttcg aacaaaactt gccaaaagga gaacaagctt ttcagcttta ttcactggga
    25561 acaccaaatg gtgtgaaggt tactatctta ttggaagaat tactagaagc tggttttaag
    25621 gaagcggctt acgacttgta taagattgct atcatggatg gggatcaatt cggctcagac
    25681 tttgtgaagc tcaatccaaa ttccaagatt ccagccttat tggaccagtc aggtactgaa
    25741 aacgtaagag tctttgagtc tgctcatatt cttctttacc ttgctgagaa atttggagcc
    25801 tttttaccaa gtaatcctgt ggaaaaggta gaagttttga attggctatt ctggcaagca
    25861 ggtgcagcac cttttctagg tgggggattt ggacatttct tcaattatgc tcctgaaaaa
    25921 ttggaatatc ctattaaccg ttttacgatg gaagtgaaac gccagttgga tttattggat
    25981 aaggaattgg ctcagaaacc ttatattgca ggcaatgact atacgattgc agatattgct
    26041 atctggtctt ggtatggaca gttagttcaa ggaaatcttt accaaggttc tgcaaaattc
    26101 ttggatgcct caagttatca aaatctagta aaatgggcag aaaaagttgc caatcgtcca
    26161 gctgttaagc gtggcttgga agtaacttat acagaaatta aatagcaaaa aagtcatctt
    26221 cggatggctt tttgctacag ttgagggaaa gggtttgcag ttatcggctt ttgtgctata
    26281 attgctataa tatagtaaaa agcaagagga gtgtgaggaa gtggaaaaga aaattgtgaa
    26341 ggatatctta tttttatctc aagtgtctca gccggcaagt caggaggacc tttatcttgc
    26401 cagagatttg caggatacac tcttagcaaa tcgtgatacc tgtgttggtc tagctgccaa
    26461 tatgattggg gtgcagaagc gcgtgattat ctttaatctt ggcttggttc ctgtggtcat
    26521 gtttaaccca gtgcttctgt cctttgaagg atcctatgag gcagaagaag gctgtttgtc
    26581 cttggtagat gtgagatcaa ctaagcgtta tgaaaccata aggcttgcct atcgtgacag
    26641 caagtggcag gaacagacca ttaccttgac aggcttccca gctcagattt gccagcatga
    26701 gctggatcac ttggaaggac gaatcattta ggaggaaagc aaatgaaacg aatagtcttt
    26761 gaacttattt ttatcgcaac gacctggtat atctttttac cgccccttaa cctgaccagc
    26821 tgggaatttc tcttcttcct ctgtgggcat ttgttagttg tggcaatatt atttggcttt
    26881 ggcaagggga taaaccttgt caaaacggtt catgtgcgcc acggtaaggc ggaagctgcc
    26941 ttaaatcttg agggtttcaa aatcaatcgg ttagggaaaa ttctgttagc ttcgattgga
    27001 ggaattcttc tcttggcagc tttggtttcc ttggtaactt ccagcatgtt tcaggctaaa
    27061 aattatgcca atgtagtcac ggttacggaa aaagacttta ctgaatttcc taagagtgac
    27121 accagtaagg ttcctatcct agatagaagt actgctgaaa aaattggaga ccgctacttg
    27181 ggttccctaa ccgataaggt gtcgcaatac gtagcggcag atacctatac ccaattgaca
    27241 attgatggga aaccttatcg ggtcacacca ctagaatatg cagaccctat caaatggttt
    27301 aacaatcaag ccaagggaat cggtgagtat atcaaggtgg acatggtaac tggaaatgcg
    27361 gatttggtgg acttgaagac accaatcaag tattcagact cggagtattt taaccgtgat
    27421 gtcaaacgtc acctgcgctt gaagtacccg accaaaatct ttaaaactcc atcttttgag
    27481 gtggacgatg agggcaatcc tttctatgta gcaacggttt accaaaagca atttggactt
    27541 gcggttcctc gtcctgcttc agtcattatc ttggatgcta caaatggaga aaccaaggaa
    27601 tacagcttat cagatgttcc agaatgggtg gacaggatct atccagcaga ggaaaccatt
    27661 gagcaaatta actacaacgg caagtacaag gacggtttct tgaatgccat gatttccaag
    27721 aaaaacgtga cccagactac caagggctat aattacttgt ctatcggtaa tgacatctat
    27781 ctctacacag gtgtgacgtc ggctaatgcg gatgagagta atcttggttt catccttgaa
    27841 aatatgcgaa caggagaaat cactaagtat agcttggctt ctgcgacaga agaatcagcc
    27901 cgtgaatcag cagaaggtgc tgttcaggag aaatcctaca aagcaacctt cccaatcctc
    27961 atcaacctca atgacaagcc tctctacatc atgggcttaa aggacaatgc tggcttggtc
    28021 aaagagtacg ccctggtaga cgcagtcgag taccaaaatg ttatcgttgc tactacagtg
    28081 gaagagatgc tcagcaagta tgccaataaa aacgaccttg aaattgacaa tgcaacgaca
    28141 gaaagcatca agggagtagt agcagacctc aaatcagctg ttatcaaggg agacactgtc
    28201 tacttcttta aagttgatgg caagatatac aaggtcaagg cttcagtatc cgatgacctt
    28261 ccttaccttg aaaatggtaa aaccttcgaa ggtcaagtag gaaaagacaa ttatctcaag
    28321 acctttaaag tacaataata ctcttcgaaa atctctagct ctatctgcaa cctcaaaaca
    28381 gtgttttgag cagcctgcg
//
LOCUS       DAVMEI010000011        28211 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVMEI010000011 DAVMEI010000000
VERSION     DAVMEI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.011"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            148..6849
                     /locus_tag="VYQ24_000387"
     CDS             148..6849
                     /locus_tag="VYQ24_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1967572.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTESRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDSQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLVTVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELSVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLYFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDYSENRRWTNWSARPSTNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPSKLVLERYVGPGFEVPTYYSNYQAYESGHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGDDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVATVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKVGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7096..7952)
                     /locus_tag="VYQ24_000388"
                     /pseudo
     CDS             complement(7096..7952)
                     /locus_tag="VYQ24_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8164..8523)
                     /locus_tag="VYQ24_000389"
     CDS             complement(8164..8523)
                     /locus_tag="VYQ24_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1967573.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            8615..9571
                     /locus_tag="VYQ24_000390"
                     /pseudo
     CDS             8615..9571
                     /locus_tag="VYQ24_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9555..10112
                     /locus_tag="VYQ24_000391"
     CDS             9555..10112
                     /locus_tag="VYQ24_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1967574.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10105..10359
                     /locus_tag="VYQ24_000392"
     CDS             10105..10359
                     /locus_tag="VYQ24_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967575.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTYHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10371..12425
                     /locus_tag="VYQ24_000393"
     CDS             10371..12425
                     /locus_tag="VYQ24_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1967576.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGIYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12604..13464
                     /locus_tag="VYQ24_000394"
     CDS             12604..13464
                     /locus_tag="VYQ24_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1967577.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLRDPVFKKD"
     gene            13675..14385
                     /gene="ccdA2"
                     /locus_tag="VYQ24_000395"
     CDS             13675..14385
                     /gene="ccdA2"
                     /locus_tag="VYQ24_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1967578.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14405..14971
                     /locus_tag="VYQ24_000396"
     CDS             14405..14971
                     /locus_tag="VYQ24_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1967579.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14982..16094
                     /gene="msrB"
                     /locus_tag="VYQ24_000397"
     CDS             14982..16094
                     /gene="msrB"
                     /locus_tag="VYQ24_000397"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1967580.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISEDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16407..17144
                     /locus_tag="VYQ24_000398"
     CDS             16407..17144
                     /locus_tag="VYQ24_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1967581.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17141..18832
                     /locus_tag="VYQ24_000399"
     CDS             17141..18832
                     /locus_tag="VYQ24_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1967582.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPQKGTKVEIYINRIETS"
     gene            18948..19511
                     /locus_tag="VYQ24_000400"
     CDS             18948..19511
                     /locus_tag="VYQ24_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1967583.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGHIG
                     KESSQEIGELIVETILDILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKT"
     gene            19533..25202
                     /locus_tag="VYQ24_000401"
     CDS             19533..25202
                     /locus_tag="VYQ24_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1967584.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGYLSGDILKTLGLDTV
                     LEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEPKS
                     EIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPTEEPKV
                     EQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDKSE
                     LNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAIDA
                     LNVDKTELNNTIADAKTKVKEHYSDRSWQNLQTGVTEAEKVAANTDAKQSEVNSETAS
                     LKTAISRLNTDKVELENQLKIAQGKTETDFSMESWTVLSTAKNKAQEVKDNGTATQEQ
                     INEAEKSLKTALADLSVDKTALGSAIDTATKKNKENYTNQTWAELETALTAAKSVNTN
                     ESKQSDVNEAAEKLTATMEKLVELSEKPRLTLSIEKRDIDRKATVTYTLENPANTQIK
                     SITATLKKGEEVVKDFVLTEENLKTNHLTALFEKLDYYKEYTLSTDMVYNRGNDDETE
                     SISEELIQLNLKKLELKDIQTVSLMKFENGQESQVTHLSDKPTDLSKLYLKVTSSTSK
                     DAVLAVSSIEEEIIENKKIFKIHADTPELVVRKKDGSLSKGFDYYMERVIPHDGDIYY
                     DFKDLISAMTSNPTGTFILGRDISSRNVKPDGNGKSYIKGEFKGKLLGTNDNVRHSIF
                     DLEYPLFDTIKSGVVKDIDFKHVNMVFPDSNQGDNVATIARVIKDKTKIENVNVEGYL
                     EGRDHVAGLVNNLEGNSEIENVSFTGKIKSKGGNSITAGIAGRNILSRVKRAYVNADI
                     EVHRSSNSSMLVAVNGINADASGGWGTWGRLTESVAKGTLETKQGGQAGGASSTVWPY
                     GAIDNVVSYAKVTKGKELFGSDGDLNYDWFMKKISNIFGVQGISSGDSGSDSKFTRIS
                     EEEANQKVASYNITAPNLMSDSSLLVDRLNESWKNTDQFESIQDYQAQNQLIYQNLTK
                     FTPYYNKEFIVHEGNALTPEQEILKTKKIKSIVGLKGTEFVVDGSDIDTIMLHFEDGS
                     QKRYKVTSTGKFSITNLPEYQVEDLNVMYTSEHIVHPLDSSLINNLVEELKKVELYTE
                     STYQVLGIDKDNANKLNRTKRLFLDESLDAVKTQLPTFVKTMFENEWLHINGESSGAV
                     AALRQKIMDNKTAILLALTYINRYYDVKFSDYNIKKLMLFKPTFHGEKIDLLDRLIRL
                     GSSGENRLKGSENAETFKQLFASETKQKDLVTYLDYNRSLLTNYQTTGEWFKETTKDY
                     IQFEERPSLVEEIKDAKYRVYDNLTAPYYQGYILPLLTLKNTHLAILSNYSTMTFVSR
                     EKRPNWKNEDFDKWVKYVATAHRNHVDTWYKILPDNIKGKMVKENVTAVWEGLSIPGS
                     EWVDQNAVDRKGRDYAPAREFFNLVGGPMGGWYAYHGYGAHAGGRNRVNYEVFDVLSE
                     YGISVFTHELTHVNDTWIYLGGYGRRENMGPEAYAQGLFQSPVPGQPGWGALGLNMAF
                     ERKNDGDLIYNASPTQFENRKELDSYMKNYNDTLMMVDYLEGDAVISKGKEAITKWFK
                     KVEPKVVSQTAQYDTVRQLTAEEKEKLSVSSVDDLVDQGLMSDRAVGNNTYNPADFET
                     SYIAIDYMTGIYGGGKNSVGSPGALMFKHNTFRMWGYYGFEEGVLGYASNKFKQASRD
                     EGHAGLSDNFIISKISKGEFLTMEAFKKGYFKKVVEELKTKGIRPVTINQKTYSTFEE
                     LQEGFKQAVERDLKKNQLDERETRNFKFQVFRQLLQQTDSFKTSIFR"
     gene            25263..26984
                     /gene="pabB"
                     /locus_tag="VYQ24_000402"
     CDS             25263..26984
                     /gene="pabB"
                     /locus_tag="VYQ24_000402"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1967585.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFHSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGKISQKQLMFEE
                     QHLERLRKASRYFAYPFDPEILRQKIEAECQTCDANQDYRLRISLSKSGEIEFSRQIL
                     TPLSPSFCQAKLCLQEANLNQSFTYFKTTYRPHLSIEEQEKIYHNGKGELLETSIGNL
                     VLKIDGKLYTPPIRLGILPGIYHQYLLETGQVEEKVLTLADFAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            27104..>28211
                     /locus_tag="VYQ24_000403"
     CDS             27104..>28211
                     /locus_tag="VYQ24_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1967586.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKTKPTVQSGWVGSSYYQNGKKV"
BASE COUNT         8907 a         5049 c         6209 g         8046 t
ORIGIN      
        1 aatcatatct acggcaaggc gacactgacg aagtttgaat tggattttcg cagagtgtaa
       61 attgttgaaa ctaggttgtc ataccatgta tactacttgg gttttggcag tagctaactg
      121 cactaaatat aatataagga gggttttatg gggaaaggtc attggaatcg gaaaagagtt
      181 tatagcattc gtaagtttgc tgtgggagct tgctcagtaa tgattgggac ttgtgcagtt
      241 ctattaggag gaaatatagc tggagaatct gtagtttatg cggatgaaac acttattact
      301 catactgctg agaaacctaa agaggaaaaa atgatagtag aagaaaaggc tgataaagct
      361 ttggaaacta aaaatgtagt tgaaaggaca gaacaaagtg aacctagttc aactgaggct
      421 attgcatctg agaagaaaga agatgaagcc gtaactccaa aagaggaaaa agtgtctgct
      481 aaaccggaag aaaaagctcc aaggatagaa tcacaagctt caaatcaaga aaaaccgctc
      541 aaggaagatg ctaaagctgt aacaaatgaa gaagtgaatc aaatgattga agacaggaaa
      601 gtggatttta atcaaaattg gtattttaaa ctcaatgcaa attctaagga agccattaaa
      661 cctgatgtag acgtatctac gtggaaaaaa ttagatttac cgtatgactg gagtatcttt
      721 aacgatttcg atcatgaatc tcctgcacaa aatgaaggtg gacagctcaa cggtggggaa
      781 gcttggtatc gcaagacttt caaactagat gaaaaagacc tcaagaaaaa tgttcgcctt
      841 acttttgatg gcgtctacat ggattctcaa gtttatgtca atggtcagtt agtggggcat
      901 tatccaaatg gttataacca gttctcatac gatatcacca aataccttca caaagatggt
      961 cgtgagaatg tgattgctgt ccatgcagtc aacaaacagc caagtagccg ttggtattca
     1021 ggaagtggta tctatcgtga tgtgacttta caagtgacag ataaggtgca tgttgagaaa
     1081 aatgggacaa ctattttaac accaaaactt gaagaacaac aacatggcaa ggttgaaact
     1141 catgtgacca gcaaaatcgt caatacggac gacaaagacc atgaacttgt agccgaatat
     1201 caaatcgttg aacgaggtgg tcatgctgta acaggcttag ttcgtacaga gagtcgtacc
     1261 ttaaaagcac atgaatcaac aagcctagat gcgattttag aagttgaaag accaaaactc
     1321 tggaccgttt taaatgacaa acctgccttg tacgaattga ttacgcgtgt ttaccgtgac
     1381 agtcaattgg ttgatgctaa gaaggatttg tttggttacc gttactatca ctggactcca
     1441 aatgaaggtt tctctttgaa tggtgaacgt attaaattcc atggagtatc cttgcaccac
     1501 gaccatgggg cgcttggagc agaagaaaac tataaagcag aatatcgccg tctcaaacaa
     1561 atgaaggaga tgggagttaa ctccatccgt acaacccaca accctgctag tgagcaaacc
     1621 ttgcaaatcg cagcagaact aggtttactc gttcaggaag aggcttttga tacttggtat
     1681 ggtggcaaga aaccttatga ctatggacgt ttctttgaaa aagatgccac tcacccagaa
     1741 gctcgaaaag gtgaaaaatg gtctgatttt gacctacgta ccatggtcga aagaggcaaa
     1801 aacaaccctg ctatcttcat gtggtcaatt ggtaatgaaa taggtgaagc taatggtgat
     1861 gcccactctt tagtaactgt taaacgtttg gtcaaggtta tcaaggatgt tgataagact
     1921 cgctatgtta ccatgggagc agataaattc cgtttcggta atggtagcgg agggcatgag
     1981 aaaattgctg atgaactcga tgctgttgga tttaactatt ctgaagataa ttacaaagcc
     2041 cttagagcta agcatccaaa atggttgatt tatggatcag aaacatcttc agctacccgt
     2101 acacgtggaa gttactatcg ccctgaacgt gaattgaaac atagcaatgg acctgagcgt
     2161 aattatgaac agtctgacta tgggaatgat cgtgtgggtt gggggaaaac agcaaccgct
     2221 tcatggactt ttgaccgtga caacgctggc tatgctggac agtttatctg gacaggtacg
     2281 gactatattg gtgaacctac accatggcac aaccaaaatc aaactcctgt taagagctct
     2341 tactttggta tcgtagatac agccggcatt ccaaaacatg acttctatct ctaccaaagc
     2401 caatgggttt ctgttaagaa gaaaccgatg gtacaccttc ttcctcactg gaactgggaa
     2461 aacaaagaat tagcatccaa agtagctgac tcagaaggta agattccagt tcgtgcttat
     2521 tcgaatgctt ctagtgtaga attgttcttg aatggaaaat ctcttggtct taagactttc
     2581 aataaaaaac aaaccagcga tgggcggact taccaagaag gtgcaaatgc taatgaactt
     2641 tatcttgaat ggaaagttgc ctatcaacca ggtaccttgg aagcaattgc tcgtgatgaa
     2701 tctggcaagg aaattgctcg agataagatt acgactgctg gtaagccagc ggcagttcgt
     2761 cttattaagg aagaccatgc gattgcagca gatggaaaag acttgactta catctactat
     2821 gaaattgttg acagccaggg gaatgtggtt ccaactgcta ataatctggt tcgcttccaa
     2881 ttgcatggcc aaggtcaact ggtcggtgta gataacggag aacaagccag ccgtgaacgc
     2941 tataaggcgc aagcagatgg ttcttggatt cgtaaagcat ttaatggtaa aggtgttgcc
     3001 attgtcaaat caactgaaca agcagggaaa ttcacactga cagcccactc tgatctcttg
     3061 aaatcgaacc aagtcactgt ctttactggt aagaaagaag gacaagagaa gactgttttg
     3121 gggacagaag tgccaaaagt acagaccatt attggagagg cacctgaaat gcctaccact
     3181 gttccgtttg tatacagtga tggtagccgt gcagaacgtc ctgtaacctg gtcttcagta
     3241 gatgtgagca agcctggtat tgtaacggtg aaaggtatgg ctgacggacg agaagtagaa
     3301 gctcgtgtag aagtgattgc tcttaaatca gagctatcag ttgtgaaacg tattgctcca
     3361 aatactgact tgaattctgt agacaaatct gtttcctatg ttttgactga tggaagtgta
     3421 caagagtatg aagtagatag ctgggagatt gccgaagaag ataaagctaa gttagcaatt
     3481 ccaggttctc gtattcaagc gaccggttat ttagaaggtc aaccaattca tgcaaccctt
     3541 gtggtagaag aaggcaatcc tgcagcacct gtagtgccaa ctgttactgt tggaggtgaa
     3601 gcagtaacag gtcttactag tcgacaacca atgcaatatc gtactctatc ttatggtgcc
     3661 caattgccag aagtcacagc aagtgctgaa aatgctgatg tgacagttct tcaagcaagc
     3721 gcagcaaacg gcatgcgtgc gagcatcttt attcagccta aagatggtgg ccctcttcaa
     3781 acctatgcaa ttcaattcct tgaagaagcg ccaaaaattg ctcacttgag tttgcaagtg
     3841 gaaaaagctg acagtctcaa agaagaccaa actgtcaaat tgtcggttcg agctcactat
     3901 caagatggaa cgcaagctgt attaccagct gataaagtaa ccttctctac aagtggtgaa
     3961 ggggaagtcg caattcgtaa aggaatgctt gagttgcata agccaggagc agtcactctg
     4021 aacgctgaat atgagggagc taaaggccaa gttgaactca ctatccaagc caatactgag
     4081 aagaagattg cgcaatctat ccgtcctgta aatgtagtca cagatttgca tcaaaaacct
     4141 actcttccaa caacagtaac ggttgagtat gacaaaggtt tccctaaagc tcacaaagtc
     4201 acttggcaag ctattccgaa agaaaaacta gactcctatc aaacatttga agtactaggt
     4261 aaagttgaag gaattgacct tgaagcgcgt gcaaaagtct ctgtagaagg tatcgtttca
     4321 gttgaagaag tcagtgtgac aactccaatc gcagaagcac cacaattacc agaaagcgtt
     4381 cggacatatg attcaaatgg tcacgtttca tcagctaagg ttgcatggga tgcgattcgt
     4441 atagagcaat acgctaagga aggtgtcttt acagttaatg gtcgcttaga aggtactcaa
     4501 ttaacaacta aacttcatgt tcgcgtatct gctcaaactg agcaaggtgc aaacatttct
     4561 gaccaatgga ccggttcaga attgccactt gcctttgctt cagattcaaa tccaagcgac
     4621 ccagtttcaa atgttaatga caagctcatt tcctacaata accaaccagc caatcgttgg
     4681 acaaactgga atcgtagtaa tccagaagct tcagtcggtg ttctgtttgg agattcaggt
     4741 atcttgagca aacgctccgt tgataatcta agtgtcggat tccacgaaga ccatggagtt
     4801 ggtgcaccga agtcttatgt gattgagtat tatgttggta agactgtccc aacagctcct
     4861 aaaaacccta gttttgttgg taatgaggac catgtcttta atgattctgc caactggaaa
     4921 ccagttacta atctaaaagc ccctgctcaa ctcaaggctg gagaaatgaa ccactttagc
     4981 tttgataaag ttgaaaccta tgctgttcgt attcgcatgg ttaaagcaga taacaagcgt
     5041 ggaacgtcta tcacagaggt acaaatcttt gcgaaacaag ttgcggcagc caaacaagga
     5101 caaacaagaa tccaagttga cggcaaagac ttagcaaact tcaaccctga tttgacagac
     5161 tactaccttg agtctgtaga tggaaaagtt acggcagtca cagcaagtgt tagcaacaat
     5221 ggcctcgcta ccgtcgttcc aagcgttcgt gaaggtgagc cggttcgtgt catcgcgaaa
     5281 gctgaaaatg gcgacatctt aggagaatac cgtctgtact tcactaagga taagaactta
     5341 ctttctcata aaccagttgc tgcggttaaa caagctcgct tgctacaagt aggtcaagca
     5401 cttgaattgc cgactaaggt tccagtttac ttcacaggta aagacggcta cgaaacaaaa
     5461 gatttgtcag ttgaatggga agaagttcca gcagaaaatc tgacaaaagc aggtcaattt
     5521 accgttcgag gccatgtcct tggtagtgat cttgttgctg aggtcactgt acgagtgaca
     5581 gacaaactag gcgaagctct ttcagataac cctaactatg atgaaaacag taaccaggcc
     5641 tttgcttcag caaccaatga tattgacaaa aactctcatg accgcgttga ctatctcaat
     5701 gacggagatt attcagaaaa tcgtcgttgg acaaactggt ctgcaagacc gtctaccaat
     5761 ccagaagtat cagcaggcgt aatcttccgt gaaaatggta agattgtcga acggactgtt
     5821 gcgcaagcca aacttcattt ctttgcagat agtggtacag atgcaccatc taaacttgtt
     5881 ttggaacgtt atgtcggccc aggctttgaa gtacctacct actattcaaa ctaccaagcc
     5941 tacgaatctg gacatccatt taacaatcca gaaaattggg aagctgtgcc ttatcgtgcg
     6001 gataaagaca tcgcagctgg tgatgaaatc aacgtaacat ttaaagctgt caaagccaaa
     6061 gccatgagat ggcgtatgga gcgtaaagct gacaagagcg gtgttgcgat gattgagatg
     6121 accttccttg caccaagtga attgcctcaa gaaagcactc aatcaaagat tcttgtagat
     6181 ggaaaagaac ttgctgattt cgctgaaaat cgtcaagact atcaaattac ctataaaggt
     6241 caacggccaa aagtctcagt tgaagaaaac aatcaagtag cttcaactgt ggtagatagc
     6301 ggagacgata gccttccagt acttgttcgc ctcgtttcag aaagtggaaa acaagtcaag
     6361 gaataccgta tccacttgac taaggaaaaa ccagtttctg agaagactgt tgctactgta
     6421 caagaagatc ttccaaaact cgaatttgtt gaaaaagatt tggcctacaa gacagttgag
     6481 aaaaaagatt caacactgta tctaggtgaa actcgtgtag aacaagaagg aaaagttgga
     6541 aaagaacgta tctttacagc gattaatcct gatggaagta aggaagaaaa actccgtgaa
     6601 gtggtagaag ctccgacaga ccgcatcgtc ttggttggaa ccaaaccagt agctcaagaa
     6661 gctaaaaaac cacaagtgtc agaaaaagca gatacaaaac caattgattc aagtgaagct
     6721 agtcaaacta ataaagccca gttaccaaat acaggtagtg cggcaagtca agcagcagta
     6781 gcagcaggtt tagctcttct aggtttgagt gcaggattag tagttactaa aggtaaaaaa
     6841 gaagactaga aattttgata ccttctttat cattaaagac caaagaaaaa ctcttgtcct
     6901 aaatagaaga taaagagtct gggacaaaat agttctcaac cacaaaaagc tagagatttc
     6961 caattgcgga actctagctt tttaattttg agtcgttctc ttattgtata gtagattgaa
     7021 actagaatag tacacttcta cttctaaaac attgttagaa atcgatttga ctgtccttat
     7081 cgatttgtcc tgttcttatt tcatttcact atattttagg aggttattgg ccataagtac
     7141 caatcccatg tcaattctca cttgacgctt ccctctcaga ttacatctct tgtaacccaa
     7201 acaagccttt atctgcccaa agacaggttc cacatcaatc ttgcgttgag cgaaaatctg
     7261 tctaccttgg ggagataaaa gcgcctgaca ttctttagct ttcaagtttt gatagcgttc
     7321 gttcatatac agtccctttt gagggactga ttcaggttca tcagcgtagt aaatcttgat
     7381 ttcctgttga aagtctgtct gtgttttctg gtacttgata tggtgaaaac gatagcacca
     7441 gccatcagga tgtgtgtagc tatcctcctt gtcattatag tgccaattcg ctaagttttt
     7501 agctgactgt ttataccctc tcttttgttt cttatcaaac atggcatatt taatcagaag
     7561 gggagtgatt tcttcttgaa aatagacctg tatctgttct tgaagtttgg cggagaattt
     7621 ctctgtcgtt ttcttccaca caaaactata cttgttggca ttggcttcaa tcttagttcc
     7681 gtcaataaac aaacaatcta aggtcactaa ctcttccatt tttcaatttt tcgaccagag
     7741 aaaatccctt gtgaataggc aaatagaaga gtagatacaa gcattttagg gtgataagac
     7801 gggcgatcaa aggcatgata ggaggtgtag aagtgacgtt cctccaaggt attcaccact
     7861 ttttcaatag taaagacgag atggtcttgt ggtaagaagg aactgatttc tagtggtaaa
     7921 gttgtttgat ttgtgttata gtgaatatgc atgggatagc ctttttaaat ggtttattaa
     7981 gcaattctat tttaccaaga ctatcgtaac catgcaaaaa aagcaacggc aaatccgttg
     8041 ctttttttgg agataagaga ctattgtccc agcctctttt ctttgtataa aattgtatag
     8101 tgttattgtc tatactattt tgtagagaaa gcaaaaatcc ctagaaaatg cttagggatt
     8161 cgactatttc attaagaaca attcaacaat catttctata tacatgataa gggttaataa
     8221 aaatcctgta cgccagaaga atttgatgaa tttaggatag taaaagctgc gtttttttaa
     8281 aaggaagaaa aagacgagaa taatggctag gaacgagagg gctagcccca gtctagggag
     8341 gaaattatgg gtaaaggttt tagctgtaat caagtaatac tcaaatacca agactggaaa
     8401 agccaaatcc gcaaagtttc gtcctagttt ttttaatcta aaaagtttgg ttaggataat
     8461 gcagacgact aaggtcagta tcaataataa aatagatgct aatttcatta aaatcatacc
     8521 catattgtat cataaaaaaa ggctaatgga aacgagaaac agaggaattt ataggaaagt
     8581 caggcttttg agattgtggg tgatttttgt tataatgaaa gttataaaat cttatgatac
     8641 cttgaatgat tattatcgaa aactctttgg agaaaagact tttaaagtcc ctattgatgc
     8701 gggatttgac tgtcccaatc gtgatggaac tgtggctcat ggaggctgta ctttttgtac
     8761 ggtttccggt tctggagatg ccattgtagc cccagatccg cctatccgtg agcaatttta
     8821 taaggaaatt gactttattc atcgtaagtg gccagatgtt cagaagtatc tggtttattt
     8881 tcaaaatttt accaacaccc atgaaaaggt ggaagttatt cgagagcgtt atgaacaggc
     8941 tatcaatgag ccaggtgtgg taggaatcaa tattggaacg cgcccagact gtttaccaga
     9001 cgaaaccatc gaatatttgg ctgagttatc ggagtgcatg catgtgacgg ttgaattggg
     9061 cttgcagact acttatgaag caacctctga cctgattaac cgtgcgcatt cttatgaatt
     9121 gtaggtggaa acggtcaagc gtttgagaaa gtatccaaaa attgagattg tttcccattt
     9181 gatcaatggt ttgcctggtg aaacccatga gatgatggtt gaaaatgtcc gccgttgtgt
     9241 cacggataac gatattcaag ggattaaact gcatttgctc catcttatga caaatactcg
     9301 tatgcaacga gattaccatg agggacgttt acaattgatg agtcaggacg aatatgtcag
     9361 ggtcatctgt gaccaactgg aaattattcc caaacatatc gtcatccatc gaattacagg
     9421 agatgcgcct agggatatgc tgattggtcc tatgtggagc ctcaataaat gggaagtcct
     9481 caacagcatt gagatggaga tgagacgtcg tggaagtgtt caaggatgca aggctgtaaa
     9541 acaggagttt gaaaatgaaa agaccacttg agatggcaca tgattttttg gctgaggtcg
     9601 tgacaaaaga ggatgtcgta gtggatgcga ctatgggaaa tggtcatgac acgctttttt
     9661 tagccaagct agccaagcaa gtctatgcct ttgatattca gaagcaagcc ttggaaaaga
     9721 cccaagagcg tttgcatcag gctggcttga caaatgccca gttaatcttg caaggccatg
     9781 agacactgga ccagtttgtg acaaaagcta aggcagggat ttttaatttg ggctatttgc
     9841 cgtcagctga taagtctgtc atcacccgac cgcagacaac gattgaggca ttagaaaagc
     9901 tatgtggctt acttgtcaaa ggtggacgaa ttgctattat gatttactat ggtcatgaag
     9961 gaggcgacct cgagagagat gctgtcttgg attttgtgat ccagttgaac caacaagagt
    10021 acacagctgc catttaccga actttaaacc aagtcaacaa cccgccgttt ttagtgatga
    10081 ttgaaaaatt agagagatac agacatggat aaacaatacc tacacgaaaa gctggatgcc
    10141 atgcgccaga attttgttga atcaacctac cacgaacgag cggtgggcgt gctagaccaa
    10201 gcgcatatga gcaaaaaaat gcttaaaatc aagaaaaaat tagttgctct tgaaatggaa
    10261 cggtgccaga gaaaaattga gcacaaagac tgttccaaga ttgaccaaaa aatcaaagag
    10321 cagaaggaga tatttgaatc ctgttgtaaa aaagattaag gaggacgtgc gtggaattac
    10381 tgatttacct catcctattt ttactggtct tgattgtctc aagtacaacc aataagctcc
    10441 tacccttttt gcctctccct ttggtgcaga ttcttttggg aattgtgatt ggtctctttt
    10501 tacccaatac tgactttcac cttaatacgg agttgttttt ggcactggtt atcggcccct
    10561 tgcttttccg agaggctgaa gaagcagatg ttacggctat tttaaaacac tggcgaatca
    10621 ttgtttatct catatttcca gtgattttta tctcgaccct gagtttgggt ggcttggccc
    10681 atcttctttg gttcagcctt cccttggcag cttgcttggc tgttggggca gcccttggtc
    10741 ctacggactt ggtggccttt gcctctcttt cggagcgttt tagctttcct aagcgcgtgt
    10801 ccaatattct taagggcgaa ggactcttga atgatgcttc tggtttggtg gcttttcagg
    10861 tagctttgac agcttggaca actggagctt tttctctggg gcaagctagc agttcactca
    10921 tcttttcaat cctaggcggt tttttaattg gatttttaac agccatgacc aaccgcttcc
    10981 tccatacctt cttgctaagt gtgcgtgcaa cggatattgc cagtgaactt ttattagaat
    11041 tgagtttgcc tcttgtgacc ttctttctgg cagaagaagt tcatgtttca ggtattattg
    11101 ccgtcgtagt tgctggaatt ttaaaggcaa gtcgtttcaa gaaaatcacg ctcctcgaag
    11161 cccaagtgga tacggtgacc gagacggtct ggcatacagt gacctttatg ctcaacggtt
    11221 ctgtctttgt gattttaggg atggagctgg aaatgatagc agaacctatc ttgaccaatc
    11281 caatctataa tcctctactt ttattgctat ctcttatcgc ccttaccttt gtcctctttg
    11341 tcattcgttt tattatgatc tatggctatt atgcctatag aacccgacgc ctcaagaaaa
    11401 agctaaataa gtatatgaag gacatgtttc tcttgacctt ttcaggtgtt aagggaacgg
    11461 tgtcgattgc tacgattctc ttgataccaa gtaatctaga acaggagtat cctctcttgc
    11521 ttttccttgt tgcaggtgtg acgcttgtta gctttttaac aggtctcttg gtcttgcctc
    11581 atctttctga tgaagaggaa gaaagcaagg attatctcat gcatatcgcc attttgaatg
    11641 aagtaacgct agagttggaa aaagagttgg aagacaccag aaataaactt tccctctatg
    11701 cggctattga caattatcat ggacgtattg aaaatctcat tttaagccaa gaaaaccagg
    11761 atgatcaaga agactgggct gctttgaaac tcttgattct tagtattgaa agtgatggtt
    11821 tggaacaggc ctatgaagag gggaatatta gcaatcgtgc ttaccgagtt taccaacgtt
    11881 atctgaaaaa tatagaacaa ggaatcaatc gtaaacttgc ctcaagactg acctattatt
    11941 ttcttgtttc cttgaggatt ttacgttttc tccttcatga agtttttact cttggaaaga
    12001 ccttccgtag ctggaaggac aaggagcaaa gccgtctccg tgctcttgat tatgaccaaa
    12061 ttgcagagct ctatcttgcc aatacagaga tgattattga aagtttggaa aacctgaagg
    12121 gaatctacag acgctctttg attagtttta tgcaggagtc tcgtcttcga gaaacagcta
    12181 ttatcagcag tggtgccttt gtcgaacggg ttatcaatcg tgtcaaaccc aacaatatcg
    12241 atgaaatgct gagaggctat tatctggagc gcaagttgat tttcgaatac gaagaaaaac
    12301 gattaattac aactaagtat gccaagaaat tacgacaaaa tgtaaataac ttagagaact
    12361 attccttgaa ggaagctgcc aataccctgc cgtatgatat ggtggaattg gtaagaagaa
    12421 attagttaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt ggattatata
    12481 tgtgactgac ttcgtcagtt tcatctacaa cctcaaagca gggctttgag caacctgcgg
    12541 ctagcttcct agtttgctct ttgattttca ttgagtataa gattataaat gaaggagtat
    12601 gacatgaaaa agtggtggaa agagctgata gacaagcctt tattaaaagc ttttttgcat
    12661 tattatcaag catcagatag tgagttgacc agtgtcgcag tagcctacta ttggttgatt
    12721 tcgattttcc ccctgctttt ggtggtggtc aatatcctcc cctattttca gattcctgtg
    12781 ggagaatttc tgtgctttat gaaagacgtt ctgcccccaa gcctctatga aggtgtggaa
    12841 aaaatagcca gagaagtttt gacgcaacct tcaacaggtt tattgagttt ttctgtttta
    12901 tcggcgttat ggagtttttc aaaatctatg aatttcttgc aaaaagcttt taacaaggct
    12961 tatggcgtcg aaaagagtcg tggacttatt tcccatcaga tgctaagtct cctagtcagt
    13021 tttggcttgc agctcctctt tgcgtttgcc ttgtttttga tcttatttgg tcagatgatt
    13081 ttggctttac ttgctcatta ttggacaaaa gatggcatta tctatcaggc tttacaaggg
    13141 ttggcaggtc ctctgattta cgccctcctc tttgctatct tggtcatgct ttattatttt
    13201 cttcccaata tatctaacag gaaaattagc tataccctac caggcagtgc ctttgttctc
    13261 ttagtgattt taggtttact aactctgttc tctagttatc tcaattacta tgtccatcat
    13321 ttggtggatg tccgaatttt aggttccgtt cttcttgttg ttatgatgtt ttggtttatc
    13381 ttgattgcta aaattgttat cctaggcgcg gttatcaatg ccagtatgca gagtttgaga
    13441 gatccggtct ttaaaaaaga ctaactattt atccattaca aaagcgcata gtatcaaaac
    13501 tttatagaac cttgatacta tgcgcttttg tgctttgaca gttttttgta tagtttttgt
    13561 ccaagatttc taaatcatct gattaaattc ctattttccc ctatcattgt ccctgttttt
    13621 tcgaaaatgg aaggagtata ataaatctat caataaaatg ataggaggaa ctttatgggt
    13681 catattttct tttttctaag tgtctttttg gcagggattc tatccttctt ttctccttgt
    13741 atcttacctt tgttaccggt ctatacagga gtgttactag atgataagga tggtgctcag
    13801 gcttctagcg gcaaattttc aatctcagtt actagtttat tacgaactct ggcctttata
    13861 gcaggaattt cctttatatt tattttgttg ggctatggag ctggtttttt aggcgatttg
    13921 ctttatgctt cttggttcca atatcttact ggggcaatta ttatccttct tggtttgcac
    13981 caaatggaga ttctacactt taaggggctt tataaggaaa agaggctaca actgcaagga
    14041 caggggcaaa atggtaaggg ctatagtcag gcatttttat tgggcttgac ctttagtttt
    14101 gcttggacgc cttgcgtggg gccggttctg gggtctgttt tggccttggc ggcttcaggt
    14161 ggttcaggag cttggcaggg agctggtctc atgttggtgt atacgctggg cttggcgcta
    14221 ccattcttgc ttctagctct gacctctagt tatgttttga aacatttccg aaaacttcat
    14281 ccctatctcg gaatcctcaa aaaagtgggt ggttttctca ttattgtgat gggactcttg
    14341 gttctgtttg gaaatgcttc aattttaagt caattatttg aataaaatgg aaaggaatat
    14401 caatatgaaa aaatggcaaa catgtgttct tggagcaggt tcgctccttt gtttgacggc
    14461 ttgttcaggc aagtccgtga ctagtgaaca ccaaacgaaa gatgaaatga agacggagca
    14521 gacagctagt aaaacaagcg cagctaaagg gaaagaggtg gctgattttg aattgatggg
    14581 agtagatggc aagacctacc gtttatctga ttacaagggc aagaaagtct atctcaaatt
    14641 ctgggcttct tggtgttcca tctgtctggc tagtcttcca gatacggatg agattgctaa
    14701 agaagctggt gatgactatg tggtcttgac agtagtgtca ccaggacata agggagagca
    14761 atctgaagcg gactttaaga attggtataa gggattggat tataaaaatc tcccagtcct
    14821 agttgaccca tcaggcaaac ttttggaaac ttatggtgtc cgttcttacc caacccaagc
    14881 ctttatagac aaagaaggca agctggtcaa aacacatcca ggattcatgg aaaaagatgc
    14941 aattttgcaa actttgaagg aattagccta ggaggtgtct tatgaatgat aagttaaaaa
    15001 tcttcttgtt gctaggagta ttttttctag ccataaccgg tttctatgtt ctattgatac
    15061 gaaatgcagg gcagacagat gcctcgcaaa ttgaaaaggc ggcagttagc caaggaggaa
    15121 aagcagtgaa aaaaacagaa attagtgaag acgcagactt gcacgaaatt tatctagctg
    15181 gaggttgttt ctggggagtg gaggaatatt tctcacgcgt tcccggggtg acggatgccg
    15241 tttcaggcta tgcaaatggt agaggagaaa caaccaagta cgaattaatt aaccaaacag
    15301 gtcatgcaga aaccgtccat gtcacctatg atgccaagca aatttctctc aaggaaatcc
    15361 tgcttcacta tttccgcatt atcaatccaa ccagcaaaaa taaacaagga aatgatgtgg
    15421 ggacccagta ccgtactggt gtttattaca cagatgacaa ggatttggaa gtgattaacc
    15481 aagtctttga tgaggtggct aagaaatacg atcaacctct agcagttgaa aaggaaaact
    15541 tgaagaattt tgtggtggct gaggattacc atcaagacta tctcaagaaa aatccaaatg
    15601 gctactgcca tatcaatgtt aatcaggcgg cctatcctgt cattgatgcc agcaaatatc
    15661 caaaaccaag tgatgaggaa ttgaaaaaga ccctgtcacc tgaggagtat gcagttaccc
    15721 aggaaaatca aacagaacga gctttctcaa accgttactg ggataaattt gaatccggta
    15781 tctatgtgga tatagcaact ggggaacctc tcttttcatc aaaagacaaa tttgagtctg
    15841 gttgtggctg gcctagtttt acccaaccca tcagtccaga tgttgtcacc tacaaggaag
    15901 ataagtccta caatatgacg cgtatggaag tgcggagccg agtaggagat tctcaccttg
    15961 ggcatgtctt tacggatggt ccacaggaca agggcggctt acgttactgt atcaatagcc
    16021 tctctatccg ctttattccc aaagaccaaa tggaagaaaa aggctacgct tatttactag
    16081 attatgttga ttaatactct tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt
    16141 atgtagggtt actgacttcg tcagttttat ctacaacctc aaagctatac tttgagctga
    16201 cttcgtcagt tttatctaca acctcaaagc tatactttga gctgacttcg tcagttttat
    16261 ctacaacctc aaagcggtac tttgagaaac ctgtggctat cttcctagtt tactttttga
    16321 tttttattga gtataagagg tctttcctaa gcagttagag gagaattttg ctatactgat
    16381 actagtaagt ggtaaaggag cagagcatga cctacacaat cttaatcgta gaagatgaat
    16441 atctggtaag acaaggtttg actaaactgg tcaatgtagc agcctacgat atggaaatca
    16501 tcggtcaggc tgaaaatgga aggcaggctt gggaattgat ccaaaagcag gtgccagata
    16561 tcattttaac cgatatcaac atgcctcatc taaatggcat ccagttggcc agtctggtac
    16621 gagaaaccta tcctcaggtt catttggtct ttttaacagg ttacgatgat tttgattatg
    16681 ccttgtctgc tgtcaaacta ggtgtggacg actacctgct caaacccttt tctcgtcagg
    16741 atattgagga aatgttgggg aaaatcaaac aaaaactaga caaggaagag aaagaagagc
    16801 agttacaaga tttattgact aataggtttg aaggaaacat ggcccagaaa atccagtctc
    16861 atctggctga tagccaattt agtttaaagt ctttagccag tgacttaggt tttagtccga
    16921 cctatctgag ttccttgatt aagaaagagt tgggcttgcc ttttcaggat tatctggtga
    16981 gagaacgtgt taaacaagcc aagctcttgc ttttaactac agatctgaag atttatgaga
    17041 tcgcagagaa ggttggtttt gaagatatga actattttac ccaacgtttt aagcagattg
    17101 caggtgtgac acctcgtcag tttaagaagg gagaagaccg atgaagcgtt cttctctttt
    17161 agttagaatg gttatttcca tctttctggt ctttctcatt ctcctagctc tggttggaac
    17221 tttctactat caatcaagtt cttcagccat tgaggccacc attgagggca acagccaaac
    17281 gaccatcagc cagactagcc actttattca gtcttatatc aaaaaactag aaaccacctc
    17341 gactggtttg acccagcaga cggatgttct ggcctatgct gagaatccca gtcaagacaa
    17401 ggtcgaggga atccgagatt tgtttttgac catcttgaag tcagataagg acttgaaaac
    17461 tgttgtgctg gtgaccaaat ctggtcaggt catttctaca gatgacagtg tgcagatgaa
    17521 aacttcctct gatatgatgg ctgaggattg gtaccaaaag gccattcatc agggagctat
    17581 gcctgttttg actccagctc gtaaatcaga tagtcagtgg gtcatttctg tcactcaaga
    17641 acttgttgat gcaaagggag ccaatcttgg tgtgcttcgt ttggatattt cttatgaaac
    17701 tctggaagcc tatctcaatc aactccagtt ggggcagcag ggctttgcct tcattatcaa
    17761 tgaaaaccat gaatttgtct accatcctca acacacagtt tatagttcgt ctagcaaaat
    17821 ggaggctatg aaaccctaca tcgatacagg tcagggttat actcctggtc acaaatccta
    17881 cgtcagtcaa gagaagattg caggaactga ttggacggtg cttggcgtgt catcattgga
    17941 aaagttagac caggttcgga gtcagctctt gtggaccttg cttggggcca gtgtcacatc
    18001 tcttcttgtc tgtctctgct tagtgtggtt cagtcttaaa cgctggattg ctcctttgaa
    18061 ggatttgaga gaaaccatgt tggaaattgc ttctggtgct caaaatcttc gtgccaagga
    18121 agttggtgcc tatgaactga gagaagtaac tcgccaattt aatgctatgt tggatcagat
    18181 tgatcagttg atggtagcta ttcgtagcca ggaagaaacg acccgtcagt accaacttca
    18241 agccctttcg agccagatta atccacattt cctctataac actttggaca ccatcatctg
    18301 gatggctgaa tttcatgata gtcagcgagt ggtgcaggtg accaagtcct tggcaaccta
    18361 tttccgcttg gcgctcaatc aaggcaagga cttgatttgt ctctctgacg aaatcaatca
    18421 tgtccgccag tatctcttta tccagaaaca acgctatgga gataagctgg aatacgaaat
    18481 taatgaaaat gttgcctttg ataatttagt cttacccaag ctggtcctac aaccccttgt
    18541 agaaaatgct ctttaccatg gcattaagga aaaggaaggt cagggccata ttaaactttc
    18601 tgtccagaaa caggattcgg gattggtcat ccgtattgag gatgatggcg ttggcttcca
    18661 agatgctggt gatagtagtc aaagtcaact caaacgtggg ggagttggtc ttcaaaatgt
    18721 cgatcaacgg ctcaaacttc attttggagc caattaccat atgaagattg attctagacc
    18781 ccaaaaaggg acgaaagttg aaatatatat aaatagaata gaaactagct aactcccagt
    18841 caattctggg agttttatgc gtaattgggc aaactttcta ggttgtctat cttgctaaaa
    18901 acaagaaaaa acggtatgat agaagagtga caacagaggt aacagagatg aacgagacac
    18961 agattcaaag agaaacacgt caggtagtgg aagatgtttt agaaaagacc aatttgaagc
    19021 agggggccct ttttgtttta ggtctatctt ctagtgaggt gctaggtggt catattggga
    19081 aagaatccag tcaagagatt ggtgagctga tagtggagac tatcttggat attctaggta
    19141 gtagaggtat ccatttagca gttcaaggtt gtgagcatgt caatcgggct ctcgttgttg
    19201 aacgtcaggt ggcagagcag tttggtctgg aaattgtcag tgtccatcct actcttcatg
    19261 caggaggttc ggggcagttg gcagccttta agtttatgca ggatccagtt gaggttgaat
    19321 ttatcaaggc tcatgctgga ttggatatcg gagacactgc aattggcatg catgtcaagc
    19381 atgttcaggt tccgattcgc cctattttga gagagattgg tcatgcccat gtaacggcac
    19441 tggctagtcg tccaaaatta atcggaggtg cgcgtgcgca ctatccgcaa gatgctatta
    19501 gaaagacctg agaatgaata aaggagataa aagtgtttaa aaaagaccgt ttttcaattc
    19561 gtaagattaa gggagttgta ggctctgtat ttcttggaag ccttttgatg gctccttctg
    19621 tagtggacgc agccacctat cactatgtaa ataaagagat tatttcacaa gaagctaaag
    19681 atttaattca gacaggaaag cctgacagga atgaagttgt atatggtttg gtgtatcaaa
    19741 aagatcagtt gcctcaaaca gggacagaag catctgtttt gacagctttt ggttatctat
    19801 caggtgacat cctcaaaacg cttggcttgg acactgtttt agaagaaacc tcagcaaaac
    19861 ctggagaggt gactgtagtc gaagttgaga ctcctcaatc aacaacaaat caggagcaag
    19921 ctaggacaga aaaccaagta gtagagacag aggaagctcc aaaagaagaa gcacctaaaa
    19981 cagaagaaag tccaaaggaa gaaccaaaat cggagataaa acctactgac gacacccttc
    20041 ctaaagtaga agaggggaaa gaagattcag cagaaccagc tccagttgaa gaagtaggtg
    20101 gagaagttga gtcaaaacca gaggaaaaag tagcagttaa gccagaaagt caaccatcag
    20161 acaaaccaac agaggaacca aaagttgaac aagtaggtga accagtcgag ccaagtgaag
    20221 acgaacaggc accaacggca ccagttgagc cagaaaagca accagaagct cctgaagaag
    20281 agaaggctgt agaggaaaca ccgaaaccag aagataaaat aaagggtatt ggtactaaag
    20341 aaccagttga taaaagtgag ttaaataatc aaattgataa agctagttca gtttctccta
    20401 ctgattattc tacagcaagt tacaatgatc ttggacctgt tttagaaact gcaaaaggtg
    20461 tctatgcttc agagcctgta aaacagcctg aggtaaatag tgagacaaat aaacttaaaa
    20521 cggctattga cgctctaaac gttgataaaa ctgaattaaa caatacgatt gcagatgcaa
    20581 aaacaaaggt aaaagaacat tacagtgata gaagttggca aaacctccaa actggggtta
    20641 cagaagctga aaaagttgca gctaatacag atgctaaaca aagtgaagta aattctgaga
    20701 ctgcaagtct taaaacagca atttctagat taaatacaga taaagttgaa ctcgaaaatc
    20761 aacttaagat tgcacaggga aaaactgaaa ctgatttcag catggaatct tggactgttt
    20821 tgagtacagc taaaaataaa gctcaggaag taaaagataa cggaacagcg acccaagaac
    20881 aaataaacga agcagagaaa agtttgaaaa cagcccttgc tgacttatct gttgacaaaa
    20941 cagctttggg aagcgctatc gatacagcta ctaagaaaaa taaagagaat tatacaaatc
    21001 aaacatgggc agagttggaa acagcgctta cagcagcaaa atctgtaaat actaacgagt
    21061 ctaaacaaag cgacgttaac gaagctgctg aaaaattaac tgcaactatg gaaaaattgg
    21121 ttgaattgtc tgaaaagcca cgtttgactc tatctattga aaagagagac atagatcgaa
    21181 aagctacggt cacgtatact ttggaaaatc cagcaaatac tcaaataaaa tcaataactg
    21241 caactctgaa aaaaggagag gaagttgtta aggattttgt tttaacagaa gaaaatctta
    21301 aaactaatca tttaactgct ttgtttgaaa agctagacta ctacaaagaa tacactctat
    21361 ctacggatat ggtatataac agaggaaatg atgatgagac ggagagtatt tcagaagaac
    21421 taattcagct gaatcttaag aaactagaat tgaaagatat ccagacagtc tctctgatga
    21481 aatttgaaaa tggtcaagaa agtcaggtta cacacttgtc tgataaaccg accgatttga
    21541 gtaaattata cttaaaagtt acatcatcaa cgtctaagga tgctgtgtta gccgttagct
    21601 ctatagaaga agagattatt gaaaataaaa aaatctttaa aattcatgca gatacaccag
    21661 aattagtagt tcgtaagaaa gatggcagtc tttctaaggg atttgactat tacatggaga
    21721 gagttatccc tcatgatggt gatatttatt atgattttaa agacttgatt tcagctatga
    21781 catctaatcc tacaggtacg tttattttag ggagagatat ttcctcgcgt aatgtaaaac
    21841 cggatggaaa tggaaaatct tatatcaaag gtgaatttaa aggaaaacta cttggtacaa
    21901 atgataatgt tagacactcg atctttgact tagaatatcc attgtttgat actataaaat
    21961 caggagtcgt gaaagatatt gatttcaagc atgtcaatat ggtattccca gatagcaatc
    22021 aaggagataa cgtagcaaca attgcaagag taatcaaaga taagacaaaa attgagaatg
    22081 ttaatgttga aggttatctc gagggtcgag atcatgtagc tggtttagtt aataatcttg
    22141 aaggtaattc cgaaattgaa aatgtatcct ttacaggaaa gattaagtct aaaggtggta
    22201 attctattac agcaggtatt gcaggtagaa atatcttatc tcgtgttaaa cgtgcctatg
    22261 taaatgcaga tatagaggta catcgatcat ctaattctag catgcttgtt gcagtaaatg
    22321 gaataaacgc agatgcaagt ggtggttggg gaacctgggg tagacttact gagtcagttg
    22381 ctaaaggaac attagaaacc aagcaaggag ggcaagctgg aggagcctct tctacagtct
    22441 ggccttatgg agcaattgat aatgttgtca gctatgcaaa agtgaccaaa gggaaagaat
    22501 tatttggatc agatggtgat ttaaattatg attggtttat gaaaaaaatt agtaatattt
    22561 ttggtgttca aggcatcagc tcaggcgatt cgggtagtga ttctaaattt acgcgtattt
    22621 ctgaggaaga agcaaatcaa aaagttgctt cttataatat tacagctccc aacttgatga
    22681 gtgactcaag tcttttggta gacagactca atgaaagttg gaaaaatact gatcaatttg
    22741 aaagtataca ggattatcaa gcccaaaacc aattaattta ccagaacttg actaaattta
    22801 ctccttatta caataaagaa tttattgttc atgaaggaaa tgctctcact cctgaacagg
    22861 aaatattgaa gactaagaaa atcaaatcga ttgtagggtt gaaaggaaca gagttcgttg
    22921 tagatggcag tgatattgat actatcatgc ttcattttga ggatggtagt cagaagcgat
    22981 ataaggtaac ctctactggt aagttttcga taactaattt accggaatat caggttgaag
    23041 atttaaatgt gatgtataca tcagaacata tagtacatcc actagattcg tcactgataa
    23101 ataaccttgt tgaagaattg aaaaaagtag aattgtatac agagtctact taccaagttc
    23161 taggtattga taaagataat gctaacaaat tgaatagaac taagagatta ttcttagatg
    23221 agtcattaga tgctgttaaa acacaattgc ctacctttgt aaaaacaatg tttgaaaatg
    23281 agtggttgca catcaatgga gaatctagtg gagctgtcgc tgctttacga caaaaaatca
    23341 tggataataa gacggctata ttgcttgctt tgacttatat caatcgttat tatgatgtta
    23401 aattctcaga ctataatatt aaaaaactga tgctctttaa accaactttc catggtgaaa
    23461 agattgatct attagatcga ttaatccgac taggatcatc tggagaaaat agattaaaag
    23521 gttcagaaaa tgcagaaacg ttcaagcagt tatttgctag cgaaacgaag cagaaggatt
    23581 tggtgactta tttagactac aatcgctctt tgttgactaa ttatcaaaca acaggggaat
    23641 ggtttaaaga aacgactaag gattatattc aatttgagga acgtccatcc ttagtagagg
    23701 agattaaaga tgccaaatat cgagtttacg ataatttaac cgcaccgtat tatcaaggat
    23761 atatcctacc tctcttaacg ttgaagaata ctcatttagc aattttatcg aactatagta
    23821 ctatgacatt tgtcagccgt gaaaaacgcc ctaattggaa aaatgaagat tttgataagt
    23881 gggtgaaata tgtagcaact gctcatagaa accatgttga tacttggtat aagattttac
    23941 ctgataatat caaaggtaaa atggttaaag agaatgttac tgctgtttgg gaaggattga
    24001 gcatcccagg aagtgaatgg gtagaccaga atgctgtaga tagaaaagga agagattacg
    24061 ccccagctcg agaattcttt aatcttgtag gtggtcctat gggaggttgg tatgcttacc
    24121 atggttatgg tgcgcatgct ggaggaagaa atcgggttaa ttatgaggtg tttgatgtat
    24181 taagtgagta tggtatatcg gtctttactc atgaattgac tcacgttaat gatacttgga
    24241 tttacttagg agggtatggt agacgtgaaa atatgggacc tgaagcttat gctcagggat
    24301 tattccagtc tcctgttcca ggtcagccgg gttggggggc gcttggtctc aatatggcat
    24361 ttgaaagaaa aaatgatggt gatctaattt ataatgcctc cccaactcaa tttgaaaata
    24421 ggaaagagtt agatagttat atgaaaaatt ataacgatac tctcatgatg gtggactact
    24481 tggaaggaga tgcagttatc tctaaaggta aagaagctat tactaaatgg tttaaaaaag
    24541 ttgaacctaa ggttgttagt caaacagctc agtatgatac ggttcgtcag ttgactgctg
    24601 aagagaaaga aaaactttct gtttctagtg ttgatgattt agttgatcaa ggattaatgt
    24661 cagacagagc tgtaggaaat aatacctata atccagctga ttttgaaaca agttatattg
    24721 cgatagatta tatgactggt atttatggtg gaggaaagaa tagtgtaggt tcaccaggtg
    24781 ccttgatgtt taaacataat actttccgta tgtggggcta ctatggtttt gaagaaggtg
    24841 ttctaggata tgcttctaat aaatttaagc aagcatctag agatgaaggt catgctggac
    24901 ttagtgataa ctttattatt tctaagattt ccaaagggga atttttaact atggaagcat
    24961 ttaaaaaggg atattttaag aaagtagtag aagagttaaa aactaaaggt attcgtcctg
    25021 ttactatcaa tcaaaaaaca tatagcacct ttgaagaatt gcaagaagga tttaagcagg
    25081 ctgttgaaag agatttgaag aaaaatcagt tagatgagag ggaaacaaga aacttcaaat
    25141 tccaagtttt cagacaactt cttcaacaaa cagatagctt taaaacttct atctttagat
    25201 aatcgaaatc tctcatctgc tttgcggatg agggttttct tattttatgc tatacttaaa
    25261 gtatgcatag aaaaacagtg attgatttta ggactttggg ggagagatat acctttaccc
    25321 agcctattaa agagttgaaa acgagaaatg tagaagaagt ggcagatttg ctggcacaag
    25381 tggaaagcta ccaagagcaa ggttattatg tggtggggta tgtcagctac gaggctgcac
    25441 ctgcttttga ggaaaaatta gcagttcaca aggatcccct actgggagag tacctgcttt
    25501 attttactgt tcatgatagc gtagaaactt cccctattcc tctgacttat gaggatattg
    25561 atttgccctc aaattggcag gaagtaacgt ctgcagcaga ctatgaaaag gccattgccc
    25621 aaattcacca tcatttacgg cagggagata cctatcaggt caactacacc gtccaactta
    25681 agcaaaagtt aaatgccaat ccttttgcca tctacaatcg tatggtggta gagcaggagg
    25741 cgggctacaa tgcctatgtg gaacatgacg agatggcagt gatttccatg agcccagagc
    25801 tcttttttga gcaaaatgat cgcgagttga caacacgacc aatgaagggg acgactcagc
    25861 gtggggtaac tgaccaagaa gatcttgaac aggccagttg gttggaacag gatcccaaaa
    25921 atcgctctga aaatatgatg attgtggacc tcttgcgcaa tgatatgaac cgtatttctg
    25981 aagttgggag cgagcacgtg gagcgtctgt gtcaggtaga gcagtattca actgtttggc
    26041 agatgacttc gaccatcaag agtcagttgc gagaggatgt ggaccttgtt gaaatcttcc
    26101 actcactctt tccttgtggt tccataacgg gtgcaccgaa aattgcgaca atggagatta
    26161 tcaaggactt ggagcctcaa ccgcgtggag tctactgtgg aacgattggt ctcttgcttc
    26221 caaatggacg acggattttt aatgtggcca ttcgtaccat tcaacttcac aaaggtcaag
    26281 ccatttatgg agttggcgga gggattactt gggatagcac ttgggaatct gaataccgtg
    26341 aagttcatca aaaggcagca gttctctatc gtaaacaagc tcgcttccaa ttaattacaa
    26401 ctggaaaaat cagccaaaaa caactgatgt ttgaagaaca acatctggaa aggctgagaa
    26461 aagctagtcg gtattttgct tatccttttg acccagaaat tttgagacaa aagattgaag
    26521 cagagtgtca gacttgtgat gctaatcaag attaccgctt gcgaatttct ctcagcaagt
    26581 ctggagagat agaattcagt cgtcaaatct taacacctct tagtccaagt ttttgtcagg
    26641 ccaaactttg tctgcaagaa gcgaatttga atcaatcctt tacctatttt aaaacgactt
    26701 acagaccgca tttgagcata gaggaacaag agaagattta ccataatgga aaaggagaac
    26761 ttcttgaaac ctctatcgga aatttggttc tgaaaatcga tgggaaactc tacacaccgc
    26821 ctatccgact tggaatcttg ccaggaattt atcaccagta tttactagaa acaggacagg
    26881 tagaagagaa agtcttgacc ttggcagatt ttgcccaagc agaagctatt tacggctgta
    26941 atgcagtgag aggcttgtat gagttgcaag tgataggatt gtgagtttta gctattaata
    27001 gtttttaata tatattaaaa tatattggtg tttacttttg atgagatatc atatttgtgt
    27061 tataatgtaa ccgtaaacaa tagaaattta aaggagattt ttgatgaaat caaaaatagt
    27121 tttgggagct tcgctagcga tcgcaacact tagcttagta tctcttgtcg agattgaagg
    27181 actctcaccg tttttaatag agaatgttag cgcaaatact cattctgcaa ataaagttat
    27241 taatcataaa gtaagtattt acttagaaaa tgctgatgaa ggtaaaggtc taactgtaaa
    27301 cttttcaaca gacagtgtta gtccaaattt atttgatgag ttcgaaaaaa agagtggaat
    27361 tacaataact actatgttag tgaatgctaa gacaggtgaa gttgtagaaa aaagattaac
    27421 accaagcgtt tttttacgtt ccaatgattt aaccagtggg accatatctt cgtttatttt
    27481 tagtgaatat cctgatggag aatataaata tgttgtgtct aaaggagact ttatcgatcc
    27541 taagactcag tttaaacatc agtatagagg agaatctcca gtttttagaa tacgtaatcg
    27601 aaaatatgtg gagttgggaa caactgataa aaaattagat gaaagacgtg ataatagcgt
    27661 ttataaagat ggggttgttg aacacaaggt aaatttaagt ttaacaagtt atcaaggagg
    27721 taatggagtt actgcaattt tttcaactga tagtgttaac tctaacttac ttaattcttt
    27781 tggtgaaaag gctaaaaaag ttttgattag gtctaagtta attaatgtta agactggcga
    27841 ggttattgat gaaacgtttt ctccaaaagt aagtttaacc tctaaaattt tgaaaagtgg
    27901 ttctacagca gtattttatt ttatagattt aactgatggc gaatataaat atgttgcgta
    27961 tgaaagccag caatatactg atcctcaaac aacattgact catcagtata gaggagaatc
    28021 tcctattttt agtatcaaag atggaaaatt ttctggttta gtatctgcct caaaaccaga
    28081 cgaaaacccg aagccaacgc caaaaccgga tgagaaacca aaaccttcag caccacaaca
    28141 ggagaaaaca aaacctacag ttcagtctgg ttgggtaggt tcatcttatt atcaaaatgg
    28201 taaaaaagtc a
//
LOCUS       DAVMEI010000012        26774 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVMEI010000012 DAVMEI010000000
VERSION     DAVMEI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.012"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            137..685
                     /locus_tag="VYQ24_000404"
     CDS             137..685
                     /locus_tag="VYQ24_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1967587.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            795..1694
                     /locus_tag="VYQ24_000405"
     CDS             795..1694
                     /locus_tag="VYQ24_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1967588.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDMLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPERRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(1719..2993)
                     /gene="serS"
                     /locus_tag="VYQ24_000406"
     CDS             complement(1719..2993)
                     /gene="serS"
                     /locus_tag="VYQ24_000406"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1967589.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3207..3578)
                     /locus_tag="VYQ24_000407"
     CDS             complement(3207..3578)
                     /locus_tag="VYQ24_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1967590.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3581..4945)
                     /locus_tag="VYQ24_000408"
     CDS             complement(3581..4945)
                     /locus_tag="VYQ24_000408"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1967591.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5315..6100
                     /locus_tag="VYQ24_000409"
     CDS             5315..6100
                     /locus_tag="VYQ24_000409"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1967592.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6194..6628
                     /locus_tag="VYQ24_000410"
     CDS             6194..6628
                     /locus_tag="VYQ24_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1967593.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6628..7602
                     /locus_tag="VYQ24_000411"
     CDS             6628..7602
                     /locus_tag="VYQ24_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1967594.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7662..7886
                     /locus_tag="VYQ24_000412"
     CDS             7662..7886
                     /locus_tag="VYQ24_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1967595.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            8005..8979
                     /gene="fabK"
                     /locus_tag="VYQ24_000413"
     CDS             8005..8979
                     /gene="fabK"
                     /locus_tag="VYQ24_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1967596.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8972..9892
                     /gene="fabD"
                     /locus_tag="VYQ24_000414"
     CDS             8972..9892
                     /gene="fabD"
                     /locus_tag="VYQ24_000414"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1967597.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSNFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9926..10657
                     /gene="fabG"
                     /locus_tag="VYQ24_000415"
     CDS             9926..10657
                     /gene="fabG"
                     /locus_tag="VYQ24_000415"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1967598.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            10679..11914
                     /gene="fabF"
                     /locus_tag="VYQ24_000416"
     CDS             10679..11914
                     /gene="fabF"
                     /locus_tag="VYQ24_000416"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1967599.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEVNVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11917..12402
                     /locus_tag="VYQ24_000417"
     CDS             11917..12402
                     /locus_tag="VYQ24_000417"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1967600.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12399..12821
                     /gene="fabZ"
                     /locus_tag="VYQ24_000418"
     CDS             12399..12821
                     /gene="fabZ"
                     /locus_tag="VYQ24_000418"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1967601.1"
                     /translation="MIDIQGIKEALPHRYLMLLVDRVLEVSENTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12833..14200
                     /gene="accC"
                     /locus_tag="VYQ24_000419"
     CDS             12833..14200
                     /gene="accC"
                     /locus_tag="VYQ24_000419"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1967602.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14237..15103
                     /locus_tag="VYQ24_000420"
     CDS             14237..15103
                     /locus_tag="VYQ24_000420"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1967603.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15100..15867
                     /locus_tag="VYQ24_000421"
     CDS             15100..15867
                     /locus_tag="VYQ24_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1967604.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            16026..16211
                     /gene="briC"
                     /locus_tag="VYQ24_000422"
     CDS             16026..16211
                     /gene="briC"
                     /locus_tag="VYQ24_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1967605.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            16229..16702
                     /locus_tag="VYQ24_000423"
     CDS             16229..16702
                     /locus_tag="VYQ24_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967606.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            16715..16951
                     /locus_tag="VYQ24_000424"
     CDS             16715..16951
                     /locus_tag="VYQ24_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1967607.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16998..17420)
                     /gene="nusB"
                     /locus_tag="VYQ24_000425"
     CDS             complement(16998..17420)
                     /gene="nusB"
                     /locus_tag="VYQ24_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1967608.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17413..17802)
                     /locus_tag="VYQ24_000426"
     CDS             complement(17413..17802)
                     /locus_tag="VYQ24_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1967609.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17824..18384)
                     /gene="efp"
                     /locus_tag="VYQ24_000427"
     CDS             complement(17824..18384)
                     /gene="efp"
                     /locus_tag="VYQ24_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1967610.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(18513..19955)
                     /gene="gatB"
                     /locus_tag="VYQ24_000428"
     CDS             complement(18513..19955)
                     /gene="gatB"
                     /locus_tag="VYQ24_000428"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1967611.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(19955..21421)
                     /gene="gatA"
                     /locus_tag="VYQ24_000429"
     CDS             complement(19955..21421)
                     /gene="gatA"
                     /locus_tag="VYQ24_000429"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1967612.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            complement(21421..21723)
                     /gene="gatC"
                     /locus_tag="VYQ24_000430"
     CDS             complement(21421..21723)
                     /gene="gatC"
                     /locus_tag="VYQ24_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1967613.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            21936..23480
                     /locus_tag="VYQ24_000431"
     CDS             21936..23480
                     /locus_tag="VYQ24_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1967614.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23734..23931
                     /locus_tag="VYQ24_000432"
     CDS             23734..23931
                     /locus_tag="VYQ24_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1967615.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            24042..24218
                     /locus_tag="VYQ24_000433"
     CDS             24042..24218
                     /locus_tag="VYQ24_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1967616.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAISLAAVRDDFYTHNFNSGILLIN
                     NGMWRAENVTQDLI"
     gene            24334..24522
                     /locus_tag="VYQ24_000434"
     CDS             24334..24522
                     /locus_tag="VYQ24_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1967617.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            24679..25044
                     /locus_tag="VYQ24_000435"
     CDS             24679..25044
                     /locus_tag="VYQ24_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1967618.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            25047..26714
                     /locus_tag="VYQ24_000436"
     CDS             25047..26714
                     /locus_tag="VYQ24_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1967619.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         7687 a         5091 c         6185 g         7811 t
ORIGIN      
        1 ttggaagtgt gataaactaa tataacaacg aaaatcttat gagagaataa agctttatct
       61 tagataaact taggaatcag gattttataa gattttccaa taatattagt gtcaataata
      121 agaaaagagg tatcttatga caacatttac tatccataca gtagagtcag caccagcaga
      181 agtgaaagaa attcttgaaa cagtagaaaa agacaacaat ggctatattc ccaacctaat
      241 cggtctcttg gccaatgccc cgactgtttt agaagcctac caaattgtct catctatcca
      301 ccgtcgcaac agcctgacac ccgttgagcg tgaagtggtg caaatcacgg cagccgtgac
      361 caatggttgt gccttctgtg tcgcaggtca cacagccttt tccatcaaac aaatccagat
      421 gaatgatgac ttgattcaag ctcttcgcaa tcgtactcca attgaaacag atcctaaatt
      481 ggatacccta gctaagttta ccttggcagt tatcaatacc aagggtcgtg taggagatga
      541 agccttgtct gagtttttag aagctggcta cactcaacaa aatgccttgg atgtggtttt
      601 cggtgtcagc ctagcaatcc tctgtaacta tgccaacaac ttagctaata caccaattaa
      661 tccagaattg caaccttatg cctaattcat atctgagtaa aatgaagtct gaaataatcg
      721 gacttcgttt ttttaaatcc tcatttaagc gcttttatgt tatactgaaa ctaacatgat
      781 tattggaggt taggatgaaa aaactcccct tagtattttc aggttgtttg ctaggtttgg
      841 caggagctgg aaatcttatt ttagatatgt tgccagttct atcccatctt tttagtctga
      901 ctggtttggt tttatggatt tactttctaa ttctgcatct ctttaattgg aaagaaacca
      961 agcaagaatt gaccaagccc cctcttttgt caggaatggc aacctttcct atggctggga
     1021 tgattttatc gacctatgtc tttcgcgtat tctcttatct tcctttggta gcacaaggga
     1081 tttggtggtt ttcatttctc ttggatttga ccttgattgc tggttttacc atcaagtttg
     1141 cttgtccaga gcggagggtt catgccactc caagctggac ggttctctat gtggggatag
     1201 cagtggctgc cttgacctat cctctggtag gtattatcga aattgcctat gcgaccttga
     1261 gttttggttt tctcttgacc ttctatctct atccccttat ttatagcgat ttaaagaaac
     1321 atccactccc actagccttg cttggacaag aaggaatcta ctgtgctcct ttctctctac
     1381 tcttggcttc tctagttcga gtaggaggaa ccagcctgcc gacttgggtc ttgattgtca
     1441 tgattttggc ttctcaatcc ttctttttct ttgttttaac tcgtctgccc aacattttaa
     1501 aacaaggttt tcaaccagcc ttctcagccc tcaccttccc aaccattatc acagcgacct
     1561 cgctcaagat ggctcaggga attttgaaac ttccatttct ggattacctg gtattggctg
     1621 aaaccattat atgcctaact attttattct ttgtactagg tgcttatctg atttggttac
     1681 gaaaaaaggt ctagctagaa atagctaaac cttatttttt atggtttgat gacttcagct
     1741 ccacccatgt atggacgaag tgcttctggg atggtcacag aaccatcttc attttggtaa
     1801 ttttcaagaa ttgcagccac tgtacgtcca actgcaagtc cagaaccgtt caaggtatga
     1861 aggagtttca ccttgccatc tgcttcatca cggtaacgga tttgggcacg acgggcttgg
     1921 aaatcttctg tgtttgaaca gcttgagatt tcacggtaat tgttttgtgc tggaatccac
     1981 acttccaagt cgtaagtctt cgtagctgag aagcccatat ctccagtaga gagagcaacg
     2041 acacggtatg gaaggttgag tttttgaaga atgttttcag cgttggctgt cattttttcc
     2101 aattcttcgt aagattcttc tggtttggca aatttgacca tttcaacctt gtggaattgg
     2161 tgcaaacgga tcaagccacg cgtatcacga ccggcagaac cagcctcaga acggaatgac
     2221 ggactcatag cagtgaagta gattggaaga tctttgccgt ctaagatttc atcacggtag
     2281 tagtttgtca gaggaacttc agcagttgga atcaagacaa agttggtatc gctgagttca
     2341 aaagtatctt ccttaaattt tggatactga ccagtaccaa acatagaatc atggttgact
     2401 atgtaaggtg tgatgacttc agtatagcct tcttttccat gttcatccaa cataaagttg
     2461 tagatagcac gttccaaacg agcaccgagg cctttataaa agaggaagcg agcgcctgtt
     2521 accttaccac cgcgttccca gtcaaggata ccaaggtctt caccgagatc ccagtgagct
     2581 ttaggttcga agtcaaactc gcgtggagta ccccaacggc gaacttccac attgtcgtct
     2641 tcgtcagccc caacaggaac gctgtcagct gggatatttg gaagagtcgt tgtaaattct
     2701 gtcaatttag catcgatttc tgccaattca gcatccaagg ctttaacctc agcagataga
     2761 ttttgcatgg cagcaatctt gtcatctgta ttttccttgt tgcgcttagc ttgggcaatc
     2821 tcagcagaaa ctgtgttacg ttctgctttg agagtttcaa ccttgaccaa gatgttacga
     2881 cgtttagcat cgatttcttt catttcattc aagacagcag catctacacc acgtgtagct
     2941 aatttttctg cgacagcttc aaaatctgta cgaatacgtt tgatatctaa cataagaact
     3001 cctttatgaa aaaagcacgc ctgacaaaac gttggagtgg cagggccacg gttccatcca
     3061 acttcacagg tgtgcacttg attgtgtatg taattgttac taacggtaga atttcaccta
     3121 tccctcctat ctgctcgcag tacccgcaga ctttctgaaa gaagaagata acctacttat
     3181 ccgttgctat gattatacta aagtttctac ttttttgcaa atagattttt aaatttttgg
     3241 ctaattgtct gaatcagggt cggaagtttg acgaccttgt cattgcctag tttttcgcgt
     3301 gcaattttga gaatggcacc tgagtctttt gaagcaaaga ggaattttcc tttgtctgta
     3361 aagacttcga agtggcggct gattttgcat ccagtgacat tggctccaat ctgattgata
     3421 tggctccaag gaatctggat aaattgttcg acattgacat ctgggtaaaa ttccaaagcc
     3481 tgatctccga caaggaattt cccaactttc ccagcgatag agaggtagga agtgcctgtc
     3541 gtactgagga gtactgtttt gttaagtgat tgagccatgc ttagtcttcc ttactttctc
     3601 caaaaaaggc attgtagagg gctttaattg ctgctttctc ttggtcttta ttgacaacaa
     3661 acataataga aacttcacta gaaccttgag acatcatctg gatgttgatt ttgttttcag
     3721 atagagcgcg tgtcgcagta gcagtcactc cgatgtggct cttcattttt tcaccaacaa
     3781 tcataatgat agaaaggtcg tgttcgattt ctgcatgatc tactttagcc ttttgaacca
     3841 actgacgcag gatttcttct tccttgatgg gagttagttg gcgagaacgg agaatgatag
     3901 aaagatcgtc gatacctgtt ggcatatgtt cccaaccgat gttaagttct tccaggattt
     3961 gcagaacctt gcggccaaat ccaacctcac ggttcatgag gtatttcgac atgttaatac
     4021 tgacaaagcc tgagtcacca gcaattccca caactggaaa ttcatcatta ctgtgtttta
     4081 gaacgatacg agtacctgga tggtcagggt tgttggtatt cttgataacc agaggaattt
     4141 ttccacggta ggcaggaaga agagcctcgt catgaaggac tgagaagcct gcataggcca
     4201 actcgcgcat ttcacggtag gtcaactcag gaatcgagtg tggttggtgg ataataccag
     4261 ggtgggctgc aaagatacca tcaacgtccg taaagttttc atagaggtcg gctttgacac
     4321 cagcagcaat gatagaacct gtaatatcag aacctccacg tgagaaagta cagatttgat
     4381 tttccttagt gacaccaaag aaaccaggaa tgacaaggac ttcattggtg tttgtcaatt
     4441 cttcaatctt gtcataactt gatggaatga tgcgagcgtg accaggttca cttgtgacca
     4501 caatcccagc ttctctaggg tgcatatagc gtgcatcgat accattttgg ttaaagtagg
     4561 cagcaatcaa tttggcattg ttattttcac cggctgctag gaaagtatcg tagagaaatt
     4621 cattttcttc aataggaaga gtggccaagg cgtgaatgct tttagaaatt ttttctagca
     4681 cagctggttt tagtcccaat tcactaacca tagcagcata gcggtcgata atccagcttt
     4741 ggctcttgct aatatcgtta cccgcaacat agtcgcggta gtatttaatc agggcatccg
     4801 taaccttagt atcttcagca ttgcgtttac caggcgcaga aacgactaca aaacgacgct
     4861 ctgaatcgct tttgacgatg tttaaaactt tttctaattg actagcagag gcaagagaac
     4921 tacctccaaa tttaacaact ttcataagaa ctcctaaagt aagtatttta tacgattata
     4981 gcagaaagaa aggctttttt caatgaagaa aataagatgc gttctagcct aaagcgagcc
     5041 tttcttatcg gctgagacac tttcagtcga ttttttcttg ctttctgctc ctaaaaaaga
     5101 tatactttga cagtgaaata atttaacact caatgaaaat caaagagcaa actaggaagc
     5161 tagccgcaga ctgctcaaag cactgctttg aggttgtaaa taagactgac gaagtcagct
     5221 caaaatactg ttttgaggtt gcagatggaa gctgacgcgg tttgaagaga ttttcgaaga
     5281 gtataaactg cttataaaat aaaaaggagc cctgatggaa cacattattt atcagcttga
     5341 agaggacttg gcaatcctta ccttgaaccg tcctgaggtc gcaaatggtt ttcatattcc
     5401 catgtgtgag gagattttag aagctctgac tttggcagaa gaaaatccag ctgtgcattt
     5461 tatcttaatc aatgccaatg gaaaggtctt ctcagttggg ggagatttgg tagagatgaa
     5521 gcgggcagtg gatgaggatg atattccatc attgacaaaa atcgcagagt tggtcaatac
     5581 gatttcttat aaaatcaagc aaatagccaa acctgtttta atggaagttg acggtgctgt
     5641 tgcaggtgcc gcagcgaata tggctgttgc ggcagatttc tgtttggcga cggataaggc
     5701 taagtttatc caagcctttg ttggtgttgg tttggctcca gatgcagggg gaattcatct
     5761 cttgagccgc agtatcggtg tgacgcgtgc tgctcaatta gctatgacag gagaggcttt
     5821 aacggcagaa aaagccctag aatgggggtt ggtttaccgc gtttctgaag ctgaaaaact
     5881 tgaaaagacg agagaacagc ttcttaaaaa attaagacgt gcttcaagta attcctatgc
     5941 tgccattaag aagttggttt gggagagcca atttaaagac tggcagggtt acgctacttt
     6001 agaactgaac ctacagaaat ccttagctca aacagaggat ttcaaagaag gagtgcgagc
     6061 tcattcggag agaagaagac ctaaatttac aggaaaataa aaaatacttg catcattctt
     6121 tgaattttga tataattctt tctgtcaaat gttttgattg taaaagtttt ttgaagaagg
     6181 agggatatga aaaatggact accaacgaat caatgaatat ttaacatcta tatttaacaa
     6241 tgtccttgta attgaggaag tgaacttgag aggtagtcgt tttaaggata tctccatcaa
     6301 agaaatgcat acgattgatg tcatcggtaa ggctccagat gtgactccaa gtcaagtgtc
     6361 aaaagagttg atggtaactc ttggaactgt tacgacaagt ttgaacaatt tagagcgtaa
     6421 gggctacatt gagcgagttc ggtcagaaca ggatcgtcgt gtggtgcatc tgcatttgac
     6481 aaagaagggt cgcttgattc atagactgca taaacgcttc cacaaggcca tggtagaaaa
     6541 aattattgat ggcatgagcg aggaagaaat tgctgtcatg ggtaaaggct tgactaatct
     6601 ttaccaattt ttggaggatt tgaaataatg gcttttgcaa aaataagtca ggttgctcat
     6661 tatgtgccag agcaagtggt tacaaatcac gacttggctc agattatgga taccaatgat
     6721 gagtggattt caagtcgaac gggaatacga caaaggcata tttcaagaac agaatctacc
     6781 agtgatttgg ctacagaggt tgctaagaaa ctgatggcaa aagctggaat aacaggaaaa
     6841 gaactggatt ttatcatcct agctaccatt actccagatt cgatgatgcc ctctacagct
     6901 gctcgtgttc aagctaatat tggcgctaat aaagcctttg cttttgactt aaccgcggct
     6961 tgcagtggat ttgtatttgc tctttcaact gctgaaaagt ttatcgcttc tggtcgcttt
     7021 caaaaaggct tggtgattgg tagtgaaacc ctctctaagg cagtcgattg gtcggatcga
     7081 tcaacagctg tgttgtttgg agatggtgct ggtggtgtct tgttagaagc tagcgagcaa
     7141 gagcatttct tagctgagag tctcaatagc gatggaagtc gcagcgagtg tttaacttat
     7201 gggcattcag gtttgcattc tccattttca gatcaagaaa gtgcagattc gtttttgaag
     7261 atggatggac gcacagtctt tgattttgcc attcgagatg tagccaagtc tatcaagcag
     7321 actattgatg aatctcctat agaggtgaca gacttggatt atctgctact tcatcaagcc
     7381 aatgaccgta ttttggataa gatggctaga aaaattggtg ttgaccgagc caaacttcca
     7441 gccaatatga tggaatatgg caataccagt gcagccagta tcccgatttt actttcagag
     7501 tgtgtagaac aaggtctcat ccctttagat ggtagccaga ctgttcttct atcaggcttc
     7561 ggtggaggct tgacctgggg cacgctcatt cttacaattt aggtaatcat gtggtgaaca
     7621 cattgttata aaaaaactat ttattttaaa ggagtcctat catggcagta tttgaaaaag
     7681 tacaagaaat tatcgttgaa gaacttggaa aagacgcatc agaagtaaca cttgaatcaa
     7741 cttttgatga tttggacgca gattcattgg acttgttcca agtaatctca gaaatcgaag
     7801 atgcttttga tatccaaatc gaagcagaaa atgacttgaa aacagttggt gacttggttg
     7861 cttacgttga agagcaagca aaataagcag aatattagta gaaggagtag ggagaaaccc
     7921 actccctctt gtttaggtaa tagtttgact gtcaaattat ggtgaaatcg aactattacg
     7981 taagcaagga aggttggagg cactatgaaa acgcgtatta cagaattatt gaagattgac
     8041 tatcctattt tccaaggagg gatggcctgg gttgctgatg gtgatttggc aggggctgtt
     8101 tccaaggctg gaggattagg aattatcggt gggggaaatg ccccgaaaga agttgtcaag
     8161 gccaatattg ataaaatcaa atcattgact gataaaccct ttggggtcaa catcatgctc
     8221 ttatctccct ttgtggaaga tatcgtggat ctcgttattg aagaaggtgt taaagttgtc
     8281 acaacaggag caggaaatcc aagcaagtat atggaacgtt tccatgaagc tgggataatc
     8341 gttattcctg ttgttcctag tgtcgcttta gctaaacgca tggaaaaaat cggtgcagac
     8401 gctgttattg cagaaggaat ggaagctggg gggcatatcg gtaaattaac aaccatgacc
     8461 ttggtgcgac aggtagccac agctatatct attcctgtta ttgctgcagg aggaattgcg
     8521 gatggtgaag gtgctgcggc tggctttatg ctaggtgcag aggctgtaca ggtggggaca
     8581 cggtttgtag ttgcaaaaga gtcgaatgcc catccaaact acaaggagaa aattttaaaa
     8641 gcaagggata ttgatactac gatttcagct cagcactttg gtcatgctgt tcgtgctatt
     8701 aaaaatcagt tgactagaga ttttgaactg gctgaaaaag atgcctttaa gcaggaagat
     8761 cctgatttag aaatctttga acaaatggga gcaggtgccc tagccaaagc agttgttcac
     8821 ggtgatgtgg atggtggctc tgtcatggca ggtcaaatcg cagggcttgt ttctaaagaa
     8881 gaaacagctg aagaaatcct aaaagatttg tattacggag ccgctaagaa aattcaagaa
     8941 gaagcctctc gctgggcagg agttgtaaga aatgactaaa acagcctttt tatttgctgg
     9001 tcaaggtgcc cagtatctag ggatgggacg ggatttctat gatcagtatc cgattgtcaa
     9061 agaaacgatt gatcgagcga gtcaggtgct cggttatgat ttacgttatc tcatcgatac
     9121 ggaagaagac aaactcaatc agacccgcta tacgcaacca gccattctag cgacttcggt
     9181 tgctatctac cgtttattgc aagaaaaggg ctatcagcct gatatggttg ctggtttgtc
     9241 tcttggagaa tactctgcct tggtggcaag cggcgccttg gattttgaag atgcggttgc
     9301 cttggtagct aagcgtggag cctatatgga agaagcggct cctgctgact ctggcaagat
     9361 ggtagcagtt ctcaatacgc cagtagaggt cattgaagaa gcctgtcaaa aagcttctga
     9421 acttggagtg gttactccag ccaactataa cacacctgca caaatcgtca ttgctggaga
     9481 agtggttgca gttgatcgag cggttgaact tttgcaagaa gcaggtgcca aacgcttgat
     9541 tcctcttaag gtgtcaggtc cctttcacac cgctctcctt gagcctgcta gccagaaact
     9601 agctgaaact ctagctcagg taagtttttc aaattttact tgtcccctag tcggcaatac
     9661 agaagctgct gtgatgcaaa aagaggacat tgctcagctc ttgacgcgtc aggtcaagga
     9721 acccgttcgt ttctatgaaa gtattggggt catgcaagaa gcaggcataa gcaactttat
     9781 cgagattgga ccggggaaag tcttgtcagg ttttgttaaa aaaattgatc aaactgctca
     9841 cttagctcat gtggaagatc aagcgagttt agtagcactt ttagaaaaat agactaaaat
     9901 aagtagaagt tttgaaagga aaaaaatgaa actagaacat aaaaatatct ttattacagg
     9961 ttcgagtcgt ggaattggtc ttgccatcgc ccacaagttt gctcaagcag gagccaacat
    10021 tgtcttaaac agtcgtgggg caatctcaga agaattgctc gctgagtttt caaactatgg
    10081 tatcaaggtg gttcccattt caggagatgt atcagatttt gcagacgcta agcgtatgat
    10141 tgatcaagct attgcagaac tgggttcagt agatgttttg gtcaacaatg cagggattac
    10201 ccaagatact cttatgctca agatgacaga agcagatttt gaaaaagtgc tcaaggtcaa
    10261 tctgactggt gcctttaata tgacacaatc agtcttgaaa ccgatgatga aagccagaga
    10321 aggtgctatc attaatatgt ctagtgttgt tggtttgatg gggaatattg gtcaagctaa
    10381 ctatgctgct tctaaggctg gcttgattgg ctttaccaag tctgtggcac gcgaggtcgc
    10441 tagtcggaat atacgagtca atgtgattgc tccaggaatg attgagtctg atatgacagc
    10501 tatcttatca gataagatta aggaagctac actagctcag attccgatga aagaatttgg
    10561 gcaggcagag caggttgcag atttgacagt atttttagca ggccaagatt atctaactgg
    10621 tcaagtggtt gccattgatg gtggcttaag tatgtagcga aagctagagg tgaaaagaat
    10681 gaaactaaat cgagtagtgg taacaggtta tggagtaaca tctccaatcg gaaatacacc
    10741 agaagaattt tggaatagtt tagcaactgg gaaaatcggc attggtggca ttacaaaatt
    10801 tgatcatagt gactttgatg tgcataatgc ggcagaaatc caagattttc cgttcgataa
    10861 atactttgta aaaaaagata ccaaccgttt tgataactat tctttatatg ccttgtatgc
    10921 agcccaagag gctgtaaatc atgccaatct tgatgtagag gctcttaata gggatcgttt
    10981 tggtgttatc gttgcatctg gtattggtgg aatcaaggaa attgaagatc aggtacttcg
    11041 ccttcatgaa aaaggaccca aacgtgtcaa accaatgact cttccaaaag ctttaccaaa
    11101 tatggcttct gggaatgtag ccatgcgttt tggtgcaaac ggtgtttgta aatctatcaa
    11161 tactgcctgc tcttcatcaa atgatgcgat tggggatgcc ttccgctcca ttaagtttgg
    11221 tttccaagat gtgatgttgg tgggaggaac agaagcttct atcacacctt ttgccatcgc
    11281 tggtttccaa gccttaacag ctctctctac tacagaggat ccaactcgtg cttcgatccc
    11341 atttgataag gatcgcaatg ggtttgttat gggtgaaggt tcagggatgt tggttctaga
    11401 aagtcttgaa cacgctgaaa aacgtggagc tactatcctg gctgaagtgg ttggttacgg
    11461 aaatacttgt gatgcctacc acatgacttc tccacatcca gaaggtcagg gagctatcaa
    11521 ggccatcaaa ctagccttgg aagaagctga gatttctcca gagcaagtag cctatgtcaa
    11581 tgctcacgga acgtcaactc ctgccaatga aaaaggagaa agtggagcta tcgtagctgt
    11641 tcttggtaag gaagtacctg tatcatcaac caagtctttt acaggacatt tgctgggggc
    11701 tgcgggtgca gtagaagcta tcgtcaccat cgaagctatg cgtcataact ttgtaccaat
    11761 gacagctggg acaagtgaag tatcagatta tatcgaagtt aatgtcgttt atggacaagg
    11821 cttggagaaa gaaattccat acgctatttc aaatactttt ggttttggag gccacaatgc
    11881 agttcttgct ttcaaacgtt gggagaatag ataagtatga atttaaacga tattaaagac
    11941 ttgatgactc aatttgacca gtcaagtttg agagaatttt cttataaaaa tgggacggat
    12001 gagttgcagt ttagcaagaa tgaagcgaga cctgtgcctg aagttgcaac tcaagtcgct
    12061 ccagcacccg ttctagcaac accgagtcca gtagctccta catctgctcc agcagagact
    12121 gtagcagaag aagttccagc tccagcagaa gcaagtgtgg ctactgaggg aaatcttgta
    12181 gagagtccac ttgttggagt ggtttacttg gctgctggac cagataaacc tgccttcgtt
    12241 acagttggtg atagtgtcaa aaaaggtcaa acattggtaa ttatcgaagc catgaaagtc
    12301 atgaatgaaa tcccagctcc taaggatggt gtggtaacgg aaattctcgt ctctaacgag
    12361 gaaatggttg agtttggcaa aggattggta cgtatcaaat gatcgatatt caaggaatca
    12421 aagaagccct tccccaccgt tatcttatgc ttctagtgga ccgtgtcttg gaagtgagcg
    12481 agaataccat tgttgctatc aaaaatgtga ccatcaacga gcctttcttt aacggccact
    12541 ttcctcaata cccagttatg ccaggtgttc tgattatgga agccttggcg caaactgctg
    12601 gtgtgttgga gttatcaaaa cctgaaaata aaggaaaact ggtcttttac gctggtatgg
    12661 acaaggttaa gttcaagaag caagttgtac caggcgacca attggttatg acagcgactt
    12721 ttgtaaaacg tcgtggcacc atagctgtgg ttgaagcaaa ggctgaagtg gatggcaagc
    12781 ttgcagccag tggtatcctt acttttgcaa ttgggaacta aggaggttct ccatgtttcg
    12841 aaaaatttta attgccaatc gtggtgaaat tgcggttcgt attatccgtg cggcacgtga
    12901 attggggatt gcgacggtag cggtttattc aactgctgat aaggaagctc ttcatacgct
    12961 tttggcagat gaagcagttt gtattggtcc tggcaaggca acagagtctt atctcaatat
    13021 taatgcagtt ctatcagctg cagtcttgac tgaggcagaa gctattcacc ctggttttgg
    13081 atttctcagt gaaaattcca aatttgcgac catgtgtgaa gaagtaggta tcaagtttat
    13141 cggtccatct ggtcatgtta tggatatgat gggggataag atcaatgcgc gtgctcagat
    13201 gattaaagca ggtgtgcctg ttataccagg ttcagatgga gaagtgcata actctgaaga
    13261 agctttgatt gttgctgaaa aaattggcta tcctgttatg ctcaaggctt cagcaggtgg
    13321 aggtggtaaa gggattcgta aggttgaaaa accagatgac ctcgtttctg cctttgaaac
    13381 tgcctctagt gaggccaagg ccaattatgg caatggtgcc atgtacatag aacgggttat
    13441 ctatccagct cggcacattg aggttcaaat cctaggtgat gagcatggac atgtgattca
    13501 cttgggtgaa cgggattgtt ctcttcaaag gaataaccaa aaggttttgg aagaaagtcc
    13561 ctcgattgca atcggaaaaa cgctgcgtca tgaaataggt gctgctgctg ttcgagcggc
    13621 agagtttgtt ggctatgaga atgcaggaac cattgaattt cttcttgatg aagcaagtag
    13681 caatttctat ttcatggaga tgaatactcg tgttcaggta gaacatccag taacagagtt
    13741 tgtttcaggt gttgatatcg ttaaggaaca gatttgcatt gcggcaggtc agcctttgtc
    13801 tgttaagcaa gaagatattg tcctacgcgg tcatgccatc gagtgtcgta tcaatgcaga
    13861 aaacccagcc tttaactttg ctccaagtcc aggtaagatt actaatctct atctgccaag
    13921 tggtggagtt ggcttgcgcg tggattcagc agtttatcca ggttatacca ttccgcctta
    13981 ttatgatagt atgattgcca aaatcatagt acacggcgaa aatcgttttg acgccttgat
    14041 gaaaatgcaa cgtgccctct atgaattaga gattgaagga gtgcagacca atgcagattt
    14101 ccagcttgac ctcatttcag atcgcaatgt cattgctggg gattatgata cttccttctt
    14161 gatggaaacc ttcttaccta aatatcaaga aaaagaataa aatgacttta agagtttaaa
    14221 ccgaaaaggg gaatcaatgg ctctatttag taaaaaagat aagtatattc gaatcaatcc
    14281 caatcgttca gttagggaaa aacctcaagc taagccagag gttccagatg aattattttc
    14341 ccagtgtcca ggctgtaagc ataccatcta tcagaaggat ctgggaagtg aacgtatctg
    14401 tccgcactgt agctatacct ttcgtatttc tgcccaagaa cgcttggctt tgacgattga
    14461 tatgggaacc ttcaaagaat tgtttacagg gattgaaagc aaggatccct tgcatttccc
    14521 tggttaccaa aagaaactgg catctatgcg tgaaaaaaca ggtctgcatg aagccgttgt
    14581 gacaggaact gctcttatta aaggtcagac tgtggctctt gggattatgg attctaactt
    14641 tatcatggct tctatgggta cggttgtagg tgaaaaaatc actcgtttgt ttgagtatgc
    14701 gactgtcgaa aaattgccag ttgtcctatt cacagcctct ggtggagccc gtatgcagga
    14761 aggaatcatg agtctcatgc agatggctaa gatctctgcg gcggttaaac gtcattcaaa
    14821 tgctggtctc ttttacctga ccattttgac agatccaacg actggtggtg tgacagcttc
    14881 tttcgctatg gaaggcgata tcattctggc tgaaccacag agcttggttg gttttgctgg
    14941 gcgtcgtgtg attgaaaata cggttcgtga aagcttgcct gaggatttcc aaaaggcaga
    15001 attcctatta gaacatggct ttgtggatgc tattgtcaaa agaagagact taccagatac
    15061 gattgctagc ctagtcagat tgcatggagg gagtcctaga tgaatattgc aaaaatagtc
    15121 agagaagcgc gtgagcagag tcgcttgaca accttggatt ttgcgacagg catttttgat
    15181 gaatttatcc aattacatgg tgaccgttct tttcgtgatg atggtgcagt tgttggtggt
    15241 attggttggc ttggagatca agctgtaaca gtggttggta tccaaaaagg caagagtttg
    15301 caagacaacc tcaaacggaa ttttggccaa ccacatccag aaggctaccg aaaggcactg
    15361 cggttgatga aacaggctga gaaatttggc cgtccagttg tgacctttat caatacagca
    15421 ggtgcttatc ctggtgtcgg agcggaagaa cgtggtcaag gggaagctat cgctcgcaat
    15481 ctcatggaaa tgagtgacct gaaagttcct attatcgcca ttattatcgg tgaaggtggt
    15541 tcaggcgggg ctctggctct agctgtcgcg gaccgtgtct ggatgctgga aaattctatc
    15601 tatgccattc tcagtccaga aggctttgct tccattttat ggaaggacgg tactcgcgct
    15661 atggaagcag cagaactgat gaaaatcact tcgcatgaac tgttagaaat ggacgtggtg
    15721 gataaggtga tttctgaaat aggactttct agtaaagaac tgattaagag tgtcaaaaaa
    15781 gaactccaaa cggagctggc tagactttca caaaaaccgc tagaagagtt gctggaagaa
    15841 cgctatcaac gatttagaaa atactaagaa tgactaaatg agactagaaa ggtctcattt
    15901 tttatggaaa aatgtaattc aggtcgaaaa attgccagtt gaggagatgg gctataaaat
    15961 agacaaaaaa agaaatatac tataaatgtg agcctacaac aatctatttt aagaaggtac
    16021 ttgcaatgac aggtacaaat acatttacag ttctttcaac tgaggacttg gagcaaactt
    16081 caggtggtct tgctgtttgg gaagatggat atagtagatg gttatattat agagaatttg
    16141 ctccctatat gaggcaaggg gcacttaatt cttatataga tgcttggaag tacggcttcc
    16201 gagcagggta atctttcaaa aaagtacgat gagaaacaca aaaaaactaa gacaatttgg
    16261 aatatttttg cttattattt tactatctac ctatttacca caaacaattg gattgtatgt
    16321 gacgatcatt ttagggttag gagctgatgt ctattctctt attttaacaa tgggattagt
    16381 gggaagtttc cttctattaa tttggaggtt aaaaaagaaa aagatgctct ttatctttga
    16441 gaaaaagagc tggaactggt catttgtctt ctacttattt gcaacttatg tggtttatca
    16501 gattctcggt aatttttggg cacgctatgc tcatttgatt aatcatagga atattcatga
    16561 tgagtatttt accgtgcttt tttcaaatgg acaaccaacc tttttatcga ctatcttatc
    16621 ttttgtcctg cctgtaatca tcggtcctgt ttttgaggag actcttgata gagggtattt
    16681 tatgaatacc ttctttccct agtccaagta ctatttggat gttatcctat ctgctatcat
    16741 ctttgggatt agccatttga tactatctca cagagatcca attagtttat tatattatag
    16801 tctgattggt ttcttttttg cccttgttta tcgttctacc gacaatctga gattaacgat
    16861 tctctgccat agttttttta actttctcaa tcatgcaaaa cctatctgga tttttgttta
    16921 caattatatc tattatcatt tttttagata gtgggagcca gaaaaagtgt ttgatagata
    16981 tcaaacactt tttcttttta ttgttcttct gttacaaact ggctgagcag tccattgata
    17041 aaacgggcag atttttgatc ggagaagtcc tttgcaagct cgatagcttc attaacagca
    17101 accagctgag gagtgtcaaa tgaagtgatt tcaaagactc ccaagcgaag gaggtttctc
    17161 tccacgagcg ttaagcgttc aatggtccaa cctgctttta aatgctgagt gatttgctta
    17221 tctagttctt cctttttagc ttgaacacca gaaacgaggt ctatcaaaaa ggctggaagt
    17281 tgtacatccg tatcttcacg atcatgagta taggcgaaac gacaagcagt ttcgacatcc
    17341 gtaccgaact caaggctcat gagagcttga aaagcgcatt tacggagttg gcgtctagat
    17401 tctaatagtg gactagtcat tgaggaagtc ctcgtcaaat agatctttca attctggttt
    17461 tggtgtttta tctgggacga tacctgcaac gtgaatattg atagcagcga gttctacatc
    17521 agccatatta cggacggcat ctttgacagc tttctggata gcaaccgcta ccttaggaac
    17581 ttttactccg tactcaaggt agagatagat atctgctgtg agttcttcgt ccacgttttt
    17641 aagataaatg ccacggccga gtgaaagttt tgaaagggta tcagacactg atctgtttga
    17701 aaaagagtga accccctcta cctttgcagt agcgatagca atgatttttt caagtacacg
    17761 tggggcgata acgatttcgc caagttgttc ttcaattccc atagaatgac ctctttctag
    17821 agattaggca cgagaaacgt aagttccttc tgcagtgttg ataacgagtt tttgtcctgc
    17881 ttcgatgaag tctggaacgt ttacgacaag tccagtttcc atcgttgctg gtttaccaga
    17941 acctgtaaca gtagcacctt tgatagatgg ttgagtttca gcaactgtca actcaacagt
    18001 agtaggaacg gtgacaccga tcacttcagt tccgtagaat tggattttca catcagagtt
    18061 ttcaaggatg taaagcaatt cgttttcaac attgactaca gggatttcgt attggtcgta
    18121 agtttctgta ttcatgaagt atgctgtgtc atccattttg tacaagtatt gagctgggac
    18181 agtctcgata atagcttgtt caaatttttc ctctggacgg tagcttgtgt caaatgtaga
    18241 accagtacgg acatcacgca atttcatacg catgatcgtg tttcctttac ctggtttgtg
    18301 gtgactagct tccaaaacgc gaatcaattt gccgtcagct gtttcaaagg tcataccagc
    18361 ttttaattta cttgcttcaa tcatgttttt acctctttaa taaatattat tactcttcat
    18421 tttaccataa aatactctgg aaataaagcc aaaatagtta ttgggagttg gtagagaaga
    18481 aaaaaccaac cttagggctg gttttgttct gtctagtttt ctttcaactt cgccaattcc
    18541 tgtgcaagta gtttaagggc aacttgtggg ttggcttggc ctttggttgc cttcataagg
    18601 aatcctgtaa aggccttgtc ggcgttacgt ttgcctgact tgaagtcggc aacagcagct
    18661 tcgttatcgg caaagacttg gtggatgatt gggatcaaga tagctggatc tgaaatttga
    18721 accataccag ctttttccac gtattcacgc gcgccaccgc catttttagc tagatggaca
    18781 aagactttct tggcaatctt agatgaaata gtaccgtctt cgatgatggt aatcatttca
    18841 accaagtttt ctggtgtcaa ttcgatttgt tccagtgttt taccttcagc attcaagaac
    18901 tgagcgactt ccccttggag ccagttagag acttgtttgg catcaccacc tagggcaaca
    18961 gctttttcaa agaaatcaga agtgacttta ttagcagtca actgactagc atcgtagtct
    19021 gataaaccaa ggtcagatac ataacgcgca cgacgttctt ttggaaactc tggcaactca
    19081 gtccgcattt cctcaatcca ctcgtcagaa atttcaaaga ggggtaggtc tggttctggg
    19141 aagtagcggt agtcagcagc cccttccttg acacgcatga ggatggttgc tttattcgct
    19201 tcatcgtaac ggcgtgtttc ttggcggatt tgaccacctg agcgaagaat ttcagcctgg
    19261 cgttggactt cgtattcaag acctttacga acgtttgaga aggagttgag gttcttcaat
    19321 tcagtcttgg taccgaattt ctcttgacca taaggacgaa gggagatgtt ggcatccaca
    19381 cgcatcgaac cttcctccat cttaacgtca gaaatgccag cgtactggat aacttccttg
    19441 agggctgtca gataagcata ggcttcttca ggagaacgca tatctgcctc agatacaatc
    19501 tcaatcaagg gaaccccttg gcggttgagg tcaacataag agtagccatc tgtaccatgg
    19561 gtgtttttac cagcgtcttc ctctaggtgg gcacgttcga taccgatttt cttggtcgta
    19621 ccgtcttcta gttcgacttc aatccagccg ttataaccga ttggctcatc aaactgagaa
    19681 atttggtagg ctttgggatt atcagggtag aagtagttct tgcggtcaaa gtgcatcttt
    19741 ttgtggatgt ccatgttgag ggcaagagca gccttgattc cggcatcgac aaccccttta
    19801 ttgagaactg gcagaactcc tgggaaagac cagtcaatca cgttagtgtt ggcattttga
    19861 tcatttccga agtgggcaga agtaggtgag aagatttttg aattggtgtt gagctctacg
    19921 tggacttcaa gtccgatgac tgtttcaaag ttcattagtt gtcacctcca aaaatcacgg
    19981 gttgttgttt gtggtagcct gttgttgctt caaaggcagc agcaacttgg taaatggttt
    20041 cctcagagtg cttgggacca atcaattgca gaccgacagg tagaccttga gagaatccag
    20101 caggaatcga aattccaggc agtcctgcca agtttacagg tatggtcaat aggtcggcta
    20161 agtacatggc aactgggtca tggttgagag aatccaagtc ataggcaaca cttggagcag
    20221 ttggacccaa aatcaaatcg taatccgcga agactttttc gaaatcttga atgataagtg
    20281 tacggacctg tccagccttc ttatagtagg catcgtagta acctgatgaa agactgaaag
    20341 tacccagcat gatacggcgc ttcacttctt caccaaaacc ttggcttcgg ctgtttacat
    20401 agatttcatc aaggttggtt gcatcttctg cgcgatagcc gtaacggata ccgtcaaagc
    20461 gttgcaagtt tgatgaagct tctgatgaag cgatgatgta gtatacggca actccgtatt
    20521 tagagtgagg aaggctgact tcttcgacaa tagcacccaa tttttcaaag tgtttagcgg
    20581 catttagaat ggtttcctta acctctgggt taattccttc accgaggtat tccttaggca
    20641 aagcgatttt catacccttg atgtcttggc cgatttttga agtaaagtcg gcgatgcgga
    20701 caggagcaga agtagagtct ttagcatctt cgctggcaat agcgttgagc aagagggcat
    20761 tttccttaac agtaggagca aaaggtccaa tctggtctaa tgagctacca aaggcaatga
    20821 gaccgaaacg tgaaactgtt ccgtaggttg gtttgagacc aacgattccg ttgaaggcag
    20881 caggttggcg gatggaacca ccagtatcag aaccaagtga caagcgaact tgtcctgagg
    20941 ctacagctgc ggcagaacca cttgatgacc caccaggaac cttgctgtgg tcccaagcgt
    21001 ttttagttgc tccgtagtgt gaagtttcac ctgaaccacc catagcaaat tcgtccatgt
    21061 tggtctttcc aacgacaatc atgcccttgg tttttgcatt ggcaacagct gtcgcatcaa
    21121 agattggctc atagttgtag agcatttttg aggcagcagt tgtgagaata ccgtctgtag
    21181 agatgttatc cttaacagca agtggaattc ctgaaaggac attgtcagca tcaattccag
    21241 cttcatcaat ggctttagct tgaacaagag cttgctcctc agcgatggtg acaaatgaat
    21301 tgagggcttc ctcacgagac ttgatatttt caagtgttgc ttgggtcaat tctgttgcag
    21361 aaatttcctt agagacaagg agattgtgca actcttcaat agttttattg ttaaaagtca
    21421 ttaggcatct cctccatcgt ctaggatagc tggcaccttg atatagtagt tgtctttttc
    21481 aggtacgttt ttaaacaagc gatcacggtc tgttccttct tcggccacat cagggcggag
    21541 tacagtcttg cggtcagcca tagtcgtagt aggtgcgaca ccagttgtgt caacttcgcc
    21601 cagcaattca accatgtcaa caatcttaga caaggtggtc gcaaaggcag cagtttcttt
    21661 ttcagagaat cttaattttg aaagattggc aacgtgtgtt acctcttctt gcgtaatttt
    21721 catcttgcat cctttcgtga aatgatgatt tttagtctgt tctattttac catattttcc
    21781 tataaataag ggagggggaa gtgaaaagaa atagaaattg aaaaaaatgc taaaatccga
    21841 tataatggag tgttgaaagg aatggtaaaa tccaatgtaa aaatcattct cagctattga
    21901 aacaagaaaa atagagaatc aaagaaagag aacttatgaa tattcaagaa gaaattaaga
    21961 aacgtcgtac ctttgccatt atctcccacc cggacgcggg gaaaacaacc atcactgagc
    22021 agttactcta ctttgggggt gagattcgtg aggctggtac ggtaaaaggg aagaaaacag
    22081 ggacttttgc taaatctgac tggatggata tcgagaagca acgtgggatt tctgttactt
    22141 catctgttat gcaatttgac tacgacggca agcgcgtgaa tatcttagac acgccagggc
    22201 acgaggactt ctcagaagat acctatcgta ccttgatggc ggtggatgct gcggtcatgg
    22261 tcgtggactc tgccaagggg atcgaggctc aaaccaagaa attgtttgag gttgtgaaac
    22321 atcgtggcat tccagtcttt acctttatga acaagctgga ccgtgacggt cgtgagcctt
    22381 tggatctctt acaagaattg gaagaaatct tgggcattgc tagctaccct atgaactggc
    22441 ctatcgggat ggggaaagcc tttgagggct tgtatgacct ctataaccaa cgtttagagc
    22501 tttataaagg ggatgagcgt tttgccagtc tagaagatgg ggacaaactt tttggtagca
    22561 atcctttcta cgagcaagtc aaggatgata ttgagctttt aaatgaagcc gggaatgagt
    22621 tttcagagga agctattctg gctggagaat tgacgcctgt ctttttcggt tcagccctga
    22681 caaactttgg tgtgcagacc ttccttgaaa ccttcctcaa gtttgctcca gaaccacatg
    22741 gtcacaagaa aacagacggt gaaattgtgg atccttatga caaggatttc tcaggatttg
    22801 tctttaaaat ccaagccaac atggaccctc gtcaccgtga ccgtattgcc tttgtccgta
    22861 tcgtatctgg cgaatttgag cgcggcatga gtgtcaatct ccctcgtact ggtaaggggg
    22921 ctaaactgtc taatgttact cagtttatgg cggaaagtcg tgagaatgtg atcaatgccg
    22981 tggcaggtga tattatcggg gtttacgata ccggtactta tcaagttggg gataccttga
    23041 cagttggtaa aaacaagttt gaatttgaac cactgccaac ctttactcct gaaattttca
    23101 tgaaagtttc tgctaagaat gttatgaagc aaaaatcctt ccacaagggg attgagcaat
    23161 tggtgcaaga aggagccgtt cagctttata agaattacca aacaggtgag tacatgctag
    23221 gcgctgtagg tcaactccag tttgaagtct ttaaacaccg catggaaggc gagtacaatg
    23281 cagaagtagt tatgaaccca atgggtaaaa agaccgttcg ttggatcaag cctgaggact
    23341 tggatgaacg gatgtcgtca agtcgcaata tcttagccaa agaccgtttt gaccaaccag
    23401 tctttctctt tgaaaatgac tttgccctcc gctggtttgc ggacaagtat ccagacgtag
    23461 agttagagga gaagatgtga ctcagtacca acaattggaa ctaaagttcc aaattgttgg
    23521 acgctagtcg ctatttggcg aactagctaa ctgcctcact aactacgttt ggcaaataga
    23581 atcgatttgc caaactccgt gtcgtaatgt aaaaattgta aaccgtaatg ggacttcatc
    23641 taaaatctcc actggtatgg ttttacgaac gaataggcga aaataatggt tacgtagaaa
    23701 catatgatgg gaataaaaga aatgagataa aaagtgaaag aagagaaaaa agcgattgtc
    23761 ttaggtgcag ataatgctta tatggataaa gttgaaacaa caataaaatc tctttgtgtt
    23821 catcattata atcttaagtt ttatgttttt aatgatgacc ttccgcgaga atggttccaa
    23881 ttaatggaaa aaagactaga gactttgaat agtgagattg tgaatgttta gattgacagt
    23941 agtattctga aaggttatcg tctccccttt gagggacttt catatgcggc ttttttaggt
    24001 attttattcc aaagtatgta agcgagtctc gtgtactgta tttggattca gatattgttg
    24061 taagaaaatc gatagatgag ctgtgggatt tggacttgac ggctatatcc ttagctgcag
    24121 ttagagatga tttttataca cataatttta attctggtat tctgttaatc aacaatggca
    24181 tgtggagagc agaaaatgtc acgcaagacc tgatttaaga cacaaataaa aaatcttcca
    24241 attcccttgc aaaaaataag aaagtgtgat aacatagtac ggtatgtggt gctagcacat
    24301 ccgctatatt agatctaata ggaggaaaac acaatggcta aagtatgtta ctttacaggt
    24361 cgtaagactg tatcaggaaa caaccgttca cacgcgatga accaaacaaa acgtgccgta
    24421 aaaccaaacc ttcaaaaagt tactgttctt atcgatggta aacctaaaaa agtttgggct
    24481 tcagctcgtg ctttgaaatc aggtaaagtt gaacgcgttt aataataaaa gcaaaggaga
    24541 ccttagggtc ttttttcttt tattgtgggt ataacatcgt ttgaaaaata gagtaaatat
    24601 ccgctgatac atcattctgc ttttacactt gggctgaaat atgataaaat agagtatcaa
    24661 ctagttgagg taaaaaggat gactgtaaaa attaatacaa aagatggtca aatcgaacta
    24721 acagatgaag tgattgcaac cgtcgtaggt ggagcagcaa ctgagatttt tggtgtggtc
    24781 ggtatggcta gtaaaaatgc cctcaaagat aatttccaag ccctgctagg taaggaaaat
    24841 tattctaaag gtgtcgtcgt aaaggcagcc gaagatggca gtattgcagt tgatgtatat
    24901 accgtgttaa gctacggaac aaagattagt gaagtgtcaa aaaacattca agagcgtgtt
    24961 gcctttagct tggaaaacca gcttggaatt actgctcaga ctgtaaatgt ctacattcaa
    25021 aatatcaaag ttgtaggaga ataaccgtgt caaaaattac tactagctta tttcaagaaa
    25081 tggtgcaggc tgcatcaact cgcttgaata agcaagctga atatgtcaat tcattaaacg
    25141 tctttccagt tccagatgga gatactggga caaatatggg aatgaccatt gaaaatggtg
    25201 ctaaagaagt tgcagacaag ccagcttcta cagttggaga agtagcgagc attcttgcca
    25261 aagggctttt gatgggtgcg cgtgggaact caggagtgat tacgtctcag cttttccgtg
    25321 gattttccca agctatcaag gataaagacg agttaacagg tcaagacttg gctctggcct
    25381 tccaatcagg tgtggaagtt gcctataagg cagtgatgaa acctgttgaa ggaacgattt
    25441 tgacagtttc tcgtggagct gctatcggtg ctaagaaaaa agctgagcaa acagatgaca
    25501 ctgttgaagt catgcgcgca gccttggaag gtgctaaaac agctctagct aaaacaccag
    25561 acatgcttcc agtattgaaa gaagttggcg ttgtggactc aggtggtcaa ggattggtct
    25621 tcatctacga aggtttcctt tcagccctta ctggcgaata tattgcatct gaggactttg
    25681 tagcgactcc tgccaacatg agtgagatga tcaatgtaga gcatcataag tctgtagctg
    25741 gtcacgtagc gactgaggac atcacgtttg gttactgtac tgaaatcatg gtagctctta
    25801 agcaaggtcc aacctatgct aaagattttg actacgatga attccgtaac tacttggatg
    25861 agcttggaga ttctctcctc gttgttaacg atgatgaaat tgtcaaagtc catgttcata
    25921 cagaagatcc aggacttgtt atgcaagaag gtctcaaata tggtagcttg gtcaaggtaa
    25981 aagttgacaa tatgcgtaac caacacgaag cacaggttga gaaagaagct actcaagtta
    26041 tcaagtcggc tgaagaaaaa gagtatgctt tgattgctgt ggtggctggt aaaggtctag
    26101 cagatatctt ctgttctcaa ggcgtggatt atgttatcga aggcggtcaa accatgaacc
    26161 cttcaacaga agactttatc aaggctgttg aacaggttaa tgcccgtaac atcatcttct
    26221 tgccaaacaa caagaacatc ttcatggcag ctcaatctgc ggcagaagtt ttggagcaac
    26281 cagcggtagt ggtagaggct cgcactcttc ctcaaggtat gacaagtctt cttgcctttg
    26341 atccaagcaa gtccattgaa gaaaaccaag agcgtatgac agctgctctt agcgatgtcg
    26401 ttagcggaag cgtcacaaca gccgtgcgtg atacaacgat cgatggctta gaaatccatg
    26461 aaaacgataa tttaggtatg gtggatggaa aaattcttgt gtcaaaccct gatatgcacc
    26521 aaacattgac tgaaaccttg aaacatatgt tggatgaaga cagtgaaatc gtaaccttct
    26581 atgtcggtga agacggaagc gaagaacttg ccaatgaaat cgctcaagaa atcgtagaag
    26641 aattcgaaga cgttgaagtc gagattcacc aaggtcaaca acctgtttac ccatacctat
    26701 ttagtgtgga ataagattaa tgaaggactg agaaatcagt tcttttttct ttttagtaaa
    26761 tgaaatcggt atct
//
LOCUS       DAVMEI010000013        25075 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVMEI010000013 DAVMEI010000000
VERSION     DAVMEI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.013"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(160..1722)
                     /gene="guaA"
                     /locus_tag="VYQ24_000437"
     CDS             complement(160..1722)
                     /gene="guaA"
                     /locus_tag="VYQ24_000437"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1967620.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIRDIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1864..2562
                     /locus_tag="VYQ24_000438"
     CDS             1864..2562
                     /locus_tag="VYQ24_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1967621.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERHVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2605..3501)
                     /locus_tag="VYQ24_000439"
     CDS             complement(2605..3501)
                     /locus_tag="VYQ24_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1967622.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSAFTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3510..4445)
                     /locus_tag="VYQ24_000440"
     CDS             complement(3510..4445)
                     /locus_tag="VYQ24_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1967623.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGSVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4442..5167)
                     /locus_tag="VYQ24_000441"
     CDS             complement(4442..5167)
                     /locus_tag="VYQ24_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1967624.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLVSISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(5251..5886)
                     /locus_tag="VYQ24_000442"
     CDS             complement(5251..5886)
                     /locus_tag="VYQ24_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1967625.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(5888..6715)
                     /locus_tag="VYQ24_000443"
     CDS             complement(5888..6715)
                     /locus_tag="VYQ24_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1967626.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6737..7072)
                     /locus_tag="VYQ24_000444"
     CDS             complement(6737..7072)
                     /locus_tag="VYQ24_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEV1967627.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(7073..7604)
                     /locus_tag="VYQ24_000445"
                     /pseudo
     CDS             complement(7073..7604)
                     /locus_tag="VYQ24_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8235..8846
                     /locus_tag="VYQ24_000446"
     CDS             8235..8846
                     /locus_tag="VYQ24_000446"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1967628.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            8891..9619
                     /gene="rlmB"
                     /locus_tag="VYQ24_000447"
     CDS             8891..9619
                     /gene="rlmB"
                     /locus_tag="VYQ24_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1967629.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(9658..10569)
                     /gene="trxB"
                     /locus_tag="VYQ24_000448"
     CDS             complement(9658..10569)
                     /gene="trxB"
                     /locus_tag="VYQ24_000448"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1967630.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10635..10859)
                     /locus_tag="VYQ24_000449"
     CDS             complement(10635..10859)
                     /locus_tag="VYQ24_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1967631.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(10937..11680)
                     /locus_tag="VYQ24_000450"
     CDS             complement(10937..11680)
                     /locus_tag="VYQ24_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1967632.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11680..12480)
                     /locus_tag="VYQ24_000451"
     CDS             complement(11680..12480)
                     /locus_tag="VYQ24_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1967633.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12638..12994)
                     /locus_tag="VYQ24_000452"
     CDS             complement(12638..12994)
                     /locus_tag="VYQ24_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1967634.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13007..13519)
                     /locus_tag="VYQ24_000453"
     CDS             complement(13007..13519)
                     /locus_tag="VYQ24_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1967635.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13519..14013)
                     /locus_tag="VYQ24_000454"
     CDS             complement(13519..14013)
                     /locus_tag="VYQ24_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967636.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14148..14594
                     /locus_tag="VYQ24_000455"
     CDS             14148..14594
                     /locus_tag="VYQ24_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1967637.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14591..15238
                     /locus_tag="VYQ24_000456"
     CDS             14591..15238
                     /locus_tag="VYQ24_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1967638.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15259..15840)
                     /gene="pdxT"
                     /locus_tag="VYQ24_000457"
     CDS             complement(15259..15840)
                     /gene="pdxT"
                     /locus_tag="VYQ24_000457"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1967639.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(15841..16716)
                     /gene="pdxS"
                     /locus_tag="VYQ24_000458"
     CDS             complement(15841..16716)
                     /gene="pdxS"
                     /locus_tag="VYQ24_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1967640.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(16868..18247)
                     /locus_tag="VYQ24_000459"
     CDS             complement(16868..18247)
                     /locus_tag="VYQ24_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1967641.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETFIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18541..19464
                     /locus_tag="VYQ24_000460"
     CDS             18541..19464
                     /locus_tag="VYQ24_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1967642.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19524..20129
                     /locus_tag="VYQ24_000461"
     CDS             19524..20129
                     /locus_tag="VYQ24_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1967643.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFSDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20148..21392
                     /locus_tag="VYQ24_000462"
     CDS             20148..21392
                     /locus_tag="VYQ24_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1967644.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21615..21872)
                     /locus_tag="VYQ24_000463"
     CDS             complement(21615..21872)
                     /locus_tag="VYQ24_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1967645.1"
                     /translation="MDPKKIARINELAKKKKTEGLTSEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(21914..23950)
                     /locus_tag="VYQ24_000464"
     CDS             complement(21914..23950)
                     /locus_tag="VYQ24_000464"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1967646.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24162..25067)
                     /gene="glyQ"
                     /locus_tag="VYQ24_000465"
     CDS             complement(24162..25067)
                     /gene="glyQ"
                     /locus_tag="VYQ24_000465"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004235033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1967647.1"
                     /translation="MTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFLRAIG
                     PEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEKLGIN
                     PLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAEVTYG
                     LERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFDKFEK
                     EAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVAKTFV
                     AERKRLGYPLLDEETRAKLLAEDAE"
BASE COUNT         7391 a         5666 c         4578 g         7440 t
ORIGIN      
        1 gcagtttgaa aactacggtc taattttttg tccaaatgta aaccacttgg aactattatt
       61 caatttattc tttttagcaa atgttcccgt tttaatccca gttacagttt ttaatccttt
      121 gtaaccgttg attttacaaa gcttttaatt tttttgcgat tactcccact caactgttgc
      181 aggtggttta cttgtaatat cgtagacgat acggttaaca tgatccactt catttacgat
      241 acgtactgag attttttgaa gtacttccca tggaattttg gcaaaatcag cagtcatacc
      301 atcgatagaa gtgatagcac ggattgcaat cgtgtagtca tacgtacgac cgtcacccat
      361 aacaccgact gaacgaacgc ctgtgttaac agtgaagtat tgccaaatat cgcggtcaag
      421 tccagcttta gcgatttctt cacgaagaat agcgtctgat tcacgaacgg tttcaagttt
      481 ctcttcagtg atttcaccca tgacacggat agcaagtcct ggtcctggga atggttggcg
      541 ccatacgata tggtctggca taccaagctc tgtaccaaga gcacgaactt catccttgta
      601 aagagtattg agtggttcaa tcaattcaaa ctgcatatct tctggaagac cacccacgtt
      661 gtggtgtgac ttgatagttt gagctgtatc cgtaccagac tcgataacat ctgtatataa
      721 agtaccttga gcaaggaatt tcacatcttt gagcttgctt gcttcgtcat cgaatacata
      781 gacaaactcg ttaccgatga ttttacgttt ttgttcagga tcagaaacgc cagcaagttt
      841 gtcaaggaaa cgtttagcag cgtctgcttt gacgatattc aaaccaaact taccaccgag
      901 catgtccata acttgatcag cttcgccttt acgaagaaga ccgtggtcca cgaagataca
      961 gatcaattga tcgccaatcg ctttttggag aagaacccca acgacagatg agtcaacacc
     1021 acctgataga ccaagaagga cacgtttatc accgacggtt tcacgaattt ttttgatctg
     1081 catgtcaatg aaattatcca ttgaccagtc acctttagcc ttacaaatgt taagggcaaa
     1141 gttacgaagg atatcatttc cgtatacaga atgacgaact tctgggtgga attggatacc
     1201 gtaaatgtgt ttatctgggt tttcgatggc tgcgtatggg cagtcagctg atgtacctgt
     1261 acgaacaaag tcagcaggaa tctcagtaac cgcatcacca tggctcatca aaacagtctg
     1321 ttcatcaggt gttgattcaa aaagcgctga tggtgtgtga gttagggttg attgaccgta
     1381 ttcacgattt ccagcatcac ctgcaggaac aacttttcct ccaagtttat gggtcaataa
     1441 ctgcatacca taacagattc ccaaaattgg aattccgagt tcgaagattt ctgggtcaat
     1501 atcaaatgaa ccatcttcat atacagaatt tggaccacct gatagaataa ttcctacagg
     1561 attgacttca cgaacttcag cagctgaaat tttatggctt tttagttctg aaaaaacacc
     1621 aatatcacgg atacggcgtg aaatcagctg gttgtactgg ctaccatagt ccaatacgat
     1681 gattttttct acatcttgca aatcagttga aatgttgctc atctttttcc tttctcaaaa
     1741 gaaatatctt ttctcaatat tctatcacaa ataagggcga attaccaact aaacaaggcg
     1801 aagtttgact ttttctagtt tattggggta caatagagaa aagatagaaa tgaagtgaaa
     1861 aatatgctac ctgcttatat gaaaatccat gatcagataa aaaaggatat tgacgagcac
     1921 cgttgggcta ttggtgagag gcttcccagt gaaagagatt tagctgagca gtttgcggtc
     1981 agtcgcatga ccctccgcca agccgtatct ctattagtcg aagaaggcgt cttagagcgc
     2041 catgtaggaa gcggcacctt tgtttccagt actcgagtac aagaaaagat gcgagggaca
     2101 accagtttta ctgaaattgt caaatcccaa ggtaaagttc cctctagcca gctcatttcc
     2161 tacaaaaaaa ccattcccaa tgagcaagaa gttgccaagc taggaatttt tccaacggac
     2221 aatattatcc gaatggagcg ggtccgctat gccgaccaag ttcccctagt ttatgaagtt
     2281 gcttctattc ctgaaaaatt cattaaggac tttaaaaaag aagaaatcac cagtcatttc
     2341 ttccaaacct tgcaaaaaca tggctatcgt atcggcaaat ctcaacagac catctatgct
     2401 cgccttgcta aagaaaagat tgcccactat ttggaagttg aaaaaggaca tgctattctt
     2461 ggattgaccc aggtttccta cctagaagat ggtactgctt ttgaatacgt aaaaagtcaa
     2521 tatgtaggcg aacgctttga attttatctt gaaaataatt agattaccac gcaaaaactc
     2581 tctgtcacgg acaaagagtc ttttttattt ttctaagaat aaatctttca aagaaatcaa
     2641 agttacagca aacaatatca ttgccatacc aagaaaatca atgggataaa attgttcatt
     2701 cattattaag aaggcaaaga aaatcgccga tattggctca attgaagcca acaagcttga
     2761 cttgaccggt cctatcagac tggctccttt aaggaaagct gtataggcaa agacagtccc
     2821 gataaggata atgcctgcaa acgcaaggag aaaatcaaga ctagtcggga tatcggcctg
     2881 tagaacccct gtaaaaggaa gagcgaccaa acctgctatg accattccca caccaatgac
     2941 caagctgctc ccccactttt taatcaaggc tatgggtaaa atgatataca gagcataagt
     3001 aaaggcagaa aagagacccc agaacagacc agcaggtgtc atggataact ggtccaactg
     3061 cccatgtgtt gcgatcagga aggttcctcc gatggcgaat atgatggaaa ctatctctcc
     3121 cagtgtcggt gccaccctat ccttgataca gctataaatt aaaattccga caggacaaac
     3181 atactgaagc accgtcgctg ttcccgcatt ggtctcctga atagcagaca gataggcgaa
     3241 ttggttgaga aaaagaccaa tcagagcaaa aataagaaga gacagcaaac tctttctatc
     3301 ctttaaaaag accagtattt tatcctttgc agtagcataa gccaagagca tgagaattcc
     3361 accagcgatt aaaagacgca agttggtcaa gaccagagcc gaaattccgt gtgccattag
     3421 gtattggcca ctcgttcctg acaaccccca agcaatacca gccacaactg ttagtaaagt
     3481 cccttttaaa ctatttgaca tgtctctcct tactcttctt tgcgaatcaa gttcatcact
     3541 tgattgcggt ctaatatcca ttgagctctc tcgtcctttt cataggtacg attcgatagg
     3601 aaaatagtgg cttcttgctt ctgacgattc cacatgataa aggtacctgt atagcccgta
     3661 tggtctagcc aatctccttc caaattccat gctaaagaac gttccttgtc atccaaagga
     3721 gaaaaatttt gacttaagtc tcttgcaaaa tcatctgcta aatagtgttc taaaaagatt
     3781 tgtaaatcct ttatagtcga aaataaacca gcactcccag catgtctacc caggagacga
     3841 gccttgggat catgcactat gcctgcctct acacctctaa ctgttggaac agcaagctca
     3901 actgacccaa acttggtttc cgtcattccc caaggtttcc agacttgatc ctttaaaatc
     3961 acatccaaat cttgattaaa aattctttcc aaaataaagc ccaacagcaa aaaatggaca
     4021 tccgaataaa gaaaggctgg ctgacttcgt ctgttgagat gaaacatcgc ttcctttaat
     4081 tcaggggctg ttaaaagatc acgattagga ataaaaggat caaggtctgt tgcatgagtc
     4141 aagagctggc gaatagtgat gtctggataa tcactctcag gtaaaaaatc tattaccagt
     4201 ctatcaatat ctaattgacc tatttcccac aagaaggtac aaactgtgcc aaccccaaca
     4261 accttgctga cactagctag gtcataaact agtcctgcct cagtctgcaa gccatgctct
     4321 gggtcactct ggcctaaata gaactctgtc cattgattgt ccttaaaata cgcaaaagag
     4381 gctccgggat aaattcctgc ctcgatttgt tcttctattt ttttaataat cttggtccac
     4441 ttcatacttc ttcaaaccac aattcaacat tatttctatc tttacaaagg aagaattttt
     4501 cagacttagg aataaaatat tcagtagact caaacttctg gcgaagactt gctatgtcta
     4561 attcattgac caagaacttg agcatagata agtcccaggt aaccgtattg tccacagtca
     4621 aatcctgccc ctgagctgga ataaaatcaa gggatgcccc aatttcagat gattccaaga
     4681 aactttcgat atcagttggg agatgtaatt ccatagaaat ctcaaattta cttaaagaaa
     4741 ttgataccaa atctgtttga aattcaggct tttctcctac ttctactaga cttgctctgt
     4801 catcttccgc atgaatcaaa atcaaatcat cttctggtga gaaaatttca aaagcgtagc
     4861 cattttgacc tttatataat cgatgaatcg aatctgtttt agataagatt ccttcaattt
     4921 ctaagggatt ttccaccttg acaatcaatc tagctagttt ttttcttccc tctaccttac
     4981 gagtacgcat actgggagct tcttctaaaa ccagcttttc aagacccgtt tggtcaccta
     5041 gtgacagaaa ggccgattct tctaacaagg ccttcattcc aagggtttca atataaaatg
     5101 tttcatttaa ttttctatta ttagctttta aagtaggaat aatccgtaca atctgattta
     5161 cattcataaa ttcctccgtc actttttatt ttaaaggatt ttagaatttt ttacaagata
     5221 ttatgctcaa atttaacatt atttagctat ttagtaaaga tagtctttca aggcttttga
     5281 caaagcctga taaccagcaa tactgaggtg cagtccatca gttgtatatt cttttttgag
     5341 ttggcctgct tggtctgtca aacaatcaaa tactggcaca aattccacct gcatataggc
     5401 agatgcaagc tcttgatagg cttgattcca gttctgaatt ttttcattcg agcggatata
     5461 gactgcctgc tggtactcct ctccctcatt gacaggcaaa atggaaagca atttaatctc
     5521 tgtcaatgga taatcgcgag caacggattg aatgatagct tcgagattat tgagagcctc
     5581 attcacagga acatcctttc cgatatcatt tgtcccaatc agaagaaaaa ttttatctac
     5641 tgctccacca tatagatgag catcaaggtt ctctagtaac agtcctgtct gatagccacg
     5701 aattcctcga ttgacaatcg tctttgaagt cccaaatagc tcctgtagag gataatactc
     5761 gacaatggaa tccccaataa aaagaatgtt tggttctaca acagaaatgt gatttaggtg
     5821 acgatactta gtttgaattt tttcttgttc ctttaggagc caattttcta ataactgtac
     5881 tgccacttca ttctcctttt tctaaccagt tttccaagac ttgataaact cctccttggc
     5941 tgttggctgg tgctagagca gtcgccacag ctttggcttt ctcatcagca ttttccatcg
     6001 cataggcaat tccagccatt tcaagcattt caacatcatt ttcactatca ccaaaagcca
     6061 tgatttcttg ggatttcaag tcccagcgct tgagtaattc ctccaagccc catgctttat
     6121 gaatcccagc ttggaggata tcaatgcaac catagccact ggatacagct cggacacggc
     6181 catcaaagag agcattgatt tcttccaaaa ccgaactcaa acgttcctca ccaacaacca
     6241 tgctcatctt gagcacacca ccaaagaggt cagatgttaa ttcatccaca aattgcatcc
     6301 gttggtagaa tttttcaatc atttctggag tcataaaact ttcaagatct gtaaaaatcg
     6361 taccttcctt gacaaaatca cccttcatcc ccgttacaac aaactggtcc tgacacgctc
     6421 gacccttgaa atgagtcaaa gccttgttga caatggcgtc atcccatgtc tgagcctgaa
     6481 tcaattcatt gttttcaaaa atacgagcgc cattagcaac aaccagaacc actcgatcca
     6541 ccaagggact cagtagttgt ctcatgcggt gaatttcatt gcccgtcgca atgacaaaac
     6601 gaatgcccct ttgatccaac tgatctaaaa tcttttccaa tcgtgggaga tcaagctgac
     6661 ctctagcatc cagcaaggtc ccatccatat ctgtcgcaat taccttaatc atcatttact
     6721 ttcctctgga ttcaagctaa taccacttca atcccacatg ttcattcatt tctttatagg
     6781 ctgtatcaat tcgttcctta gtcgcttcat ctaactggtc acgatgactg ccatcctcta
     6841 taaaatcttc caaaatataa gtttggtaat gatacaaggg atatccttct ggtgaaaagg
     6901 ttccctttgg tgttcgattg acccaagtag gatgagcttt agctgttccg atagttgttt
     6961 ttccatcctt cttcttgatg gtgacatcca tgagaacacc acgttcagtc cacttagcat
     7021 tctcttcatc tcccatagat tcaatccgtt gattggaaat gaagttcccc atttaataga
     7081 caatgagctt cttatctcca tctttttcca ccgtttcaga tggttcaaca acgtgaggat
     7141 gccctccaaa gataatatcc gctccccaat cgatcatctt gtgataaaga gctttttgtt
     7201 cttcagttgg ttccaatcga tactcaacac ccatctgaag catgataatg gtgatatctg
     7261 cttccttctc tgcccgttca atttcaacct tcatcttatc ttcgtttaaa tctgaaagat
     7321 aacgattata gtcttcctga gaaatatact gctcaattcc attgaaacca taggaatagg
     7381 ctaacaatgc aaccttgata ccattcactt ccttaatgac cagcggagcc tgaacacgtg
     7441 gttcgtgcgt ataaactccg attggagtga ttccagcttt ctcaataata tcggccgttg
     7501 aaataactcc ctcaagttga aataactccc tcaagttgcg aatccaaaat atgattatga
     7561 gctaaatcca gcacatgata acctgcctcc ttaatagcat ccataacttc agcaggagca
     7621 ttaaagagaa gataacccgc taaataatga tccttattaa tggttccttc aaaatcacca
     7681 atagctaaat ctgcttgctt gagccaagga gtcacatact caaaattttc atgaaagtca
     7741 taggtaccgt cttctttttt agctgaaaag aaaagtccat cgtggtagag caaatccccg
     7801 tgggccataa ttctggcagt tttttcctct tcctactcct gagacttttg cttagtcccc
     7861 tcttgagaaa tagtatctcg tttttgacta gtcaagggat tccttggaag ctttcaaaca
     7921 acaagaccaa gcccttcata aaacgaaaag ctaaaccacc ctgatttcga ttttatcttc
     7981 tttgtatctt cattctccct actttcttat gcaagccttt tctttttatt atatcacaga
     8041 taagtatttc tttcacaatt gaattgaact tcccatctat tttctataaa tcctaaatgc
     8101 cataatgctt tcaattcttg tcattttgtg atatcatgta gaggaaatga actaatccac
     8161 agtggcttat ttcaagtata ccacttgggc tttggcagta gctaactgca ctaaatataa
     8221 tataaggaga gaaaatgtct gcaatagaac gtattacaaa agctgctcac ttaattgata
     8281 tgaacgatat tatccgtgaa gggaatccta ctctacgcgc gattgctgag gaagtcactt
     8341 tccccctatc tgaccaggaa atcatcctag gcgaaaagat gatgcaattc cttaaacatt
     8401 cccaagaccc tgtcatggct gaaaaaatgg gactccgcgg tggtgttgga ctggctgctc
     8461 cccagttaga tatctcaaaa cgcattatcg ctgttttggt acctaatatt gttgaagaag
     8521 gcgaaactcc acaggaagcc tacgatttgg aagccattat gtacaatcca aaaatcgtct
     8581 ctcactctgt tcaagatgct gctcttggcg aaggagaagg ttgcctgtct gttgaccgta
     8641 acgtgcctgg ctatgttgtt cgccatgccc gcgttactgt tgactacttt gacaaagatg
     8701 gagaaaaaca ccgtatcaaa ctcaaaggct acaactccat tgttgttcag catgaaattg
     8761 accacattaa cggtatcatg ttttacgatc gcatcaatga aaaagaccca tttgcagtta
     8821 aagatggttt actgattctt gaataaagaa aatcccgttg caagacgggg ttttgtgtta
     8881 taatagaggc atgaaaacaa atgatattgt ctatggcgtc cacgccgtta ccgaagccct
     8941 ccttgcaaat acaggaaaca aactctacct ccaagaagat ctccgaggta agaatgttga
     9001 gaaagtcaag gaactagcta cagaaaagaa ggtgtccatt tcttggacat caaaaaaatc
     9061 tctctctgag attactgaag gtgctgttca tcaaggtttt gttctacgag tgtctgaatt
     9121 tgcctatagc gagctagatt acatccttgc aaaaacacgc caagaagaaa atccacttct
     9181 attgattcta gatggtctaa ccgatcccca taatctgggt tctatcttgc gaacagccga
     9241 tgcgaccaat gtttcaggtg tcatcattcc caagcaccgt gctgtcggag taactcctgt
     9301 cgttgccaaa acagccacag gtgctattga acacgttcca attgcccgag tgacaaacct
     9361 cagtcaaacc ttagataaac tcaaggatga aggcttctgg acctttggaa cggatatgaa
     9421 cggtactcct tgccacaagt ggaatacaaa agggaaaatc gccctcatca tcggaaatga
     9481 aggaaaaggc atctctagca acatcaaaaa acaggtcgat gaaatgatta ccattccgat
     9541 gaatggacat gttcaaagcc tcaatgccag tgttgctgcg gccattctca tgtacgaagt
     9601 ttttctaaat agactataaa aaagtttcca gtcatctgat tggaaacttt tttatgatta
     9661 actatgttct gtgataaact tgtaggcttc ttgaccagcg atagctccat ctccaactgc
     9721 tgttgttact tggcgaaggt ctttcaagcg aacatctcca actgcaaaga taccgtcgac
     9781 tgcagtcttc atgtggctat ctgtcacaat ccagcctgcc tgatcttgga tattcaattc
     9841 tttaacaaaa tcgctaagag ggtccaaacc aacatagata aagacaccac cgaaggcttg
     9901 ctctgtcact tgacctgttt tcacattttc aaaaacgaca gattctactc tgttttcacc
     9961 cttgatttcc ttcactacag aatcccagat aaagctgatt ttttcattcg caaaggcgcg
    10021 atcttgtaaa accttttggg cacgaagttg gtcacgacgg tgaacaatgg taacagtctt
    10081 agcaaaacga gtcaagaaga gggcttcttc aacagctgaa tctccaccac caactaccaa
    10141 taaatcttgg tcacggaaga aagcaccatc acacacagca cagtaagaaa caccacgact
    10201 gttcagttct tcttctccag gcactcccaa aggacggtgt ttagaaccag ttgctacgat
    10261 aactgtacgt gtttcatatg tttggtcatc agtcatcact ttcttaaaat caccatggtc
    10321 ttcgacattt tcaacataac cataaatgtg ctcaacacca agattttcaa gtggttcaaa
    10381 catcttttca gccaattcag gtccactaat attagcgtat cctgggtaat tttcgatatc
    10441 agatgtatta ttcatctgac cacctggcag accaccttca atcaaagcta cttttagatt
    10501 gcttcgagca gcatacaagg ccgcagtcat ccctgcaggt ccagcaccga taataatagt
    10561 atcgtacata tagattcctt ctttcttgtt gtaactatct ttattctaac tctttcttgt
    10621 caatcacgca aggattatgc cttgaaaaca agaattaaac tcataaccgc aaaggataat
    10681 actgggacaa ccaagactcc aatcataatc atatcataga gatgggcttg gcgagccctg
    10741 gctgtcttat caactcccga catggctctc agaccaatcc aaatccctaa aaaaatcaga
    10801 acaaggatgg tggtcaagat caaactctcg aaatataaag aaaatagttg cagtagcatg
    10861 atttctctca tttctatctt ttttaaagag taaactcagc tagtccaact aactgagttt
    10921 tcctttatct attatatcaa atataagtcc gtttgtaact agcgaagaat tcttttgtcc
    10981 gctcttcttt aggggtgtgg ataatctcat ccggagttcc agactcgatg attttcccct
    11041 tatctaagaa gagaatttta tccgcaactt gggctacaaa ggacatgtca tgactgacca
    11101 aaatcatggt ctgacctgac ttagcagcat ctgcaataga cttttctact tcaccgacca
    11161 attctgggtc aagggctgaa gttggttcgt ctaagagcaa aacatctggt ttcatagcaa
    11221 gcgcacgcgc tagggcaacc cgttgcttct gtccacctga taaatggcga ggataatggt
    11281 tttcacggtc cgaaagccca accttagcca actcttcctt ggcaatctta gtcgcttctt
    11341 ggtcagataa tttcttgaca acaaccaagc cttctttcac attatcaagt gctgttcgac
    11401 gttcaaataa attaaactgt tggaaaacca tagacaactt acggcgtagg gcaaggattt
    11461 cttcttgagt gattttagaa aaatcaactg aaaaaccatc aatctgaata gagccactgt
    11521 caggtgtttc aagataattg agactgcgaa gaaaggttga ttttccagct cctgaagaac
    11581 caatcaaggc tacaacttcc cctttttgaa tatccaagtt cagatgatcc aagacagtct
    11641 gtcctgaaaa ggatttgctt aaattcgaaa tcttaatcat taacgaaggt ctcctttcac
    11701 atctgtttgc actgtatcag gtgcagaaat agccattttt ctctcgatga aacgaccgag
    11761 gctttcaatt ccgatattga ctacccaata aacaagggca acagagatga agcgttcaaa
    11821 atagcgataa tcagctccac ctagaatctg agcttgggca aagacttcca caacacccgc
    11881 actaaaagct agagatgttc ccttggtcaa accgatgagg gaattaatca aggttggagt
    11941 agctaccacc gctgcattag gaataatcac tcgtcgataa acttgcgctc gggtcatacc
    12001 cagactgcgc gccgcctcaa tctcaccagg attaactgag agaatggctg cacgaatggt
    12061 ttcactagca taagctgcct cattaaaggc aaaagcgaca atcgcaaaag ctgcagctgg
    12121 aatcgcattg atattgagac cagttcccca ttgctgattg agggctttca aagccaaagg
    12181 aattccgtag taggtcaaca tgagttgcac caaaatcggt gtccctttta agaaactaac
    12241 aaagaaggcc tgcaagggat ataaaatctt gacacgattg atcttcacaa tggcaaaaag
    12301 aagcgccaaa accaagccaa aaagggcacc accaattgtc aacataattg ttgttggaag
    12361 ttgttggaca attctaggga ttccatcaaa gaccgaacgt aggctaaaca gcttgccatc
    12421 cggaatcaat tgcatcaagt tttggtacca atctgatgct aaaatcgttg taacattcat
    12481 aaaaacatcc tcttctgata atgattcatt ctaccatgct tctgccgtca atgaaattat
    12541 ttgctacggt cagatacaga ctttgtctac ctactagttt atcatacttt gaaacaggga
    12601 agttatatat tttatctatc aaagagatag ataaaaacta tttcagtccc agttcttcat
    12661 aagattttcg ataaccgatt tgcttaacag ttccattttc tactaaaatg ggccgtttta
    12721 acaacatacc gtcacttgct agcaactcag ccgcttcttg gtttgacaaa cttcctacct
    12781 tatcttttag ccctaattca cggtatttga taccactggt gttgaaaaat tgcttcaatt
    12841 caaatcctga ggtttctagc caattcaaaa tgacttcttg gctaggtgtt tcttccacga
    12901 tatggacggc tttatagtcc acacctaatt gatttaattc ttgttttgct tttttacaag
    12961 ttgaacattt ggggtattcg ataaattcta acatgtgctt ctccttctat ttgtttatat
    13021 ctttaaaatc tacgccttca tgctccaaga gccaagcttt cttttccact cctgcagcat
    13081 agcctgtcag acgcttgcct gctcccaaca cacgatgaca aggtactagg atagaccaag
    13141 gattgcgtcc cactgctcca ccaattgctt gagcagaagc cacttgcagg tcttgagcaa
    13201 tttgtccata ggtcactgtc tgaccataag gaatgccctg taaatagtcc caaactctct
    13261 tttcaaaatt cgttccgatt ggcgccaaga gcaagttgga taaatcctga ggcttgcctt
    13321 taaagtaatc atctaagcaa gcaataactg ggtctaaaat aggatgacta acaacttctt
    13381 ctatcgtttc atctcctagt cccctctcaa aatgcttctg ctcctgaacc caaattccat
    13441 acaaataatg gtcatcagct acaagtgata gaataccaat tggtgaggag tagaggattt
    13501 ttacatactt cttttgcatt atttctttaa tttttgatag gccaagcgtt cgccatctac
    13561 tggcacctta ccaacttgtt taaaaccaag tttttcaaaa atatgttgca taaccttgtt
    13621 ttcagcatgc gtatctgagc gaaaatcaag ataatcaaaa ccttcaatca agccctctaa
    13681 gaaggtttga gcaactcctt ttccctgcac atctgctgcc acagcaatac ggtgaaagac
    13741 tagatactct gactctccag cttgccagtt tccctcataa atagcttcat aggcctcctc
    13801 tggactcttg gtcacagcag cataggctag cagttctccc tcttccaagg ctacgtaggc
    13861 ttgccctgag atgatatcat cgataataac atctgcattt ggatagccat tttgccactg
    13921 gttactacca gcatctgcta aacatttttt ggcatcctct atcacctgca tgatggcatc
    13981 tacttcattt ggaaaagcta aacgaatttc catctttttt cctcatttct gactagtttc
    14041 tcccatcata gcataattta agtctttcta caagcagtta aaacttgact ttgtattttt
    14101 gttattttat aatctagtta tcaaaactat ataaaaagga gttgaaaatg aaaactacct
    14161 tttcctaccc aaaatgggca gaaattccaa acattaacct ctatctggac caggttttgc
    14221 tctatgtcaa tcaggtctgc gcccctatct ctcctaataa agacaaaggc ctaacagcat
    14281 ctatggtcaa caattatgtg aaaaatggtt acctgacaaa gcctgacaaa aaaaaatacc
    14341 aacgccaaca gattgcccgt ttgattgcta tcacaaccct caagtctgta ttttctattc
    14401 aagaaatagc ccagacactt aatactctac aaactcaagc aagttcagac caactctacg
    14461 atgcttttgt ggactacatg aaccaaggaa ttgacccagc taaccctatt atccaaacca
    14521 gctgccaaac cgttaaactc tatcatcaaa ctctagactt aatcgatcat actcaagagg
    14581 aggtaatcca atgaacacta gtcttaaact cagcaaacaa ctcagttttg gagaggagat
    14641 tgctaatagc gtgacccatg ctgtgggtgc agtcatcatg cttatcttgc tgcctatttc
    14701 atccatctat agttatgaag cacacggatt tttatcctct atcggcgttt ccattttcgt
    14761 catcagtctc tttctcatgt tcctatcatc caccatttat cactctatgg cctatggttc
    14821 gacccacaaa tatgttttgc gaatcattga ccattctatg atttacgttg ccattgccgg
    14881 ctcatacacg cccgttgtct tgaccttgat gaataactgg tttggctatc tgattattgt
    14941 catccaatgg ggaacgacca tctttggtat tctctataaa atctttgcta aaaaggtcaa
    15001 tgagaaattt agccttgctc tttacctgat tatgggctgg ttggttctgg ctatcattcc
    15061 tgccattatc agtcaaacga cacccgtttt ctggagtctc atggtaactg gcgggctctg
    15121 ttatacagtt ggagctggat tttatgccaa gaaaaaacct tatttccaca tgatttggca
    15181 tctctttatc ctagctgcgt ccgcacttca atacatcgct attgtttatt acatgtaaaa
    15241 aagttgagaa attcaatctc aacttttttc tttacacata ttgataaagt actggtgcaa
    15301 gcgcacatca tcagtcaatt ctggatgaaa agaacttacc aacatatttt tttcttgggc
    15361 tgcaacaatt tgattgttca ctgttgctaa aatttctaca ccctcaccaa cactactgat
    15421 aatcggacca cggataaagg tcattggaat cttgccaact cccttacatt ctgcttccgt
    15481 gtagaaactt cctaattggc gcccataagc attacgctcg accaccatat ccatagttcc
    15541 tagatgactc tctttctgag aagtgatttc cttagccagc aaaattaagc ccgcacaggt
    15601 cccaaacact ggtaagccag atagaatggc ttctcggatg ggaagtagca tgttctggtc
    15661 acgtaagagc ttgcccatgg ttgtagactc accaccaggc aaaatcaaac ccgacaagtc
    15721 actctgatct tgctgaaaat catctagatt tctgagttct acactctcga cacctaattg
    15781 atctagcact tttgcatgtt ctgcaaaggc cccttgcaag gccaatattc cgattttcat
    15841 ctattttcct cgttcagcca tgagaatttg gatttcattt tcattaatac caaccatggc
    15901 ttctcctaaa tcttcagaga tttgagctag aatttgagga ttacggaagt tagtcacagc
    15961 cttaacaatg gcactcgctc gtttaacagg atctcctgac ttgaaaatac ctgaaccgac
    16021 aaagaccccc tctgccccta attgcatcat taacgcagca tctgctggcg ttgcaacacc
    16081 tccagcagcg aaatttacaa ctggcaattt tccatgttca tgaacatatt ggaccaattc
    16141 tacagggact tgcaaatcct tggcagcaac ataaagctcg tcctcacgta agttttgaat
    16201 gcggcgaatt tcctgattca tcatacgcat atgacgaaca gcttggacta tatcccctgt
    16261 ccctggttct cctttggtac gaatcatgga agcaccttca gcgatacgac gcaaggcttc
    16321 acccaaatcc ttagcaccac agacaaaagg aacttggaat tctttcttgt ccacatggaa
    16381 acggtcatca gctggagata gaacttcact ctcgtcgata taatcaattt caatagcctc
    16441 taaaatctga gcttcaacaa aatgcccgat tctgacctta gccattactg gaatactaac
    16501 cgcttcttgg atttccttaa tcatctttgg gtcgctcatg cgggaaactc ctccagctgc
    16561 acgaatatca gccggaattc gttccaaggc catcacagct gccgcaccag cagcttctgc
    16621 gatacgagcc tgttcaggat tctgcacatc cataataaca ccacccttga gcatctgtgc
    16681 caagttttta tttagttcat aacgattttc agtcatttgt tttatcctca tcatttgtat
    16741 tggtagctac cttttcattt taagtcagaa cagaatgaag cacaagataa aaaaactata
    16801 attgtatggg gacagattag ctaaaaacta tctgacctta acttaaggcc agatagctca
    16861 ttcattttta tttttcagcc gtaagggcag ccattgtgat gtagttgtat ggtttgttga
    16921 agtgtggcaa gaagaagagg tctgtcaatg ccaatttatc aattgtcaca tgctcttgga
    16981 tagcaagtga gaacatgtgg attcccatgc taattgcaat atcatgtgaa accatttggg
    17041 caccaagaat ttcacggcta tctttgtcaa agacaatctt aattgctact tcatggttgt
    17101 catgtttcat gaattctggt ttttgaagat cgttaaagcc tgtttcagtt gcgttgtaac
    17161 cagcagcttt cgctttttca agagtcaaac cagttgaaac catgtgaaga ccgtagattg
    17221 agataccatt tgaaccttga acaccgattc cttccaattc atgtccacaa gcattgtagg
    17281 caccaacgat accagtgcga acagcatttg aagcaagagc gatatagctt gtatctttac
    17341 gagcattgtc ataaacagtc gcacagtcac caacagcgta aacgcctggg ataaatgttt
    17401 cttgtttctt gtctacaagg aaggcaccgt tgcggaagag ttcgatctta ccatctgcaa
    17461 gggctgtgtt tggacggaaa ccaactgcaa ggataaccat atccacgtca aagctttctt
    17521 tgtcagtaat caagcgttca actttaccgt caccttcgat tgctttaaca gtttgaccta
    17581 gagccaagcg gatgttgtga tcttccaagt tcttcgccat catttgtgtg aagtctttgt
    17641 catagtaacc gttcaagaca gtatcaacga tatcaacaag gacaacttct tttccaagac
    17701 gttcaaaggc ttcagcaagt tcaacaccga tgtaaccacc accaacaacg gcgatacggt
    17761 cgaggtgttg gctcttgtca gaaagtttat tgataacttc ttcagcattt tggtacaatt
    17821 tcacgaattg tacgttttca agagttgctt taaattcgcg gtttccttta acaatttcaa
    17881 caccttcgat tggtggcaag attggtgtag aacctgtagc gaaaatcaat ttttcgtatg
    17941 attctttgtg ctcttttcct tcaacttccg ctgtaactac tttgttatca tagtcgattg
    18001 aaagaacagg tgagttcatg taaactttag cacctttagc ttccaatttt tctttatcag
    18061 aatagaacaa gccttcagca ccgtcaattt gttcaccaat ccaaagagcc attccacatc
    18121 ctaggaaaga gatgttagag ttttggtcaa atacaacaat ttcgttctca tttccaaaat
    18181 tatccaacat ggtattgata catgctgtac cagcgtggtt agcaccgact acaacgattt
    18241 tactcataaa aatttcctgc cttcattcta aatttacctt actagtatac catttaccgt
    18301 tagcgtttac aaggggggta ctattatttc agcatttttt atcaaaaata attccccatt
    18361 tatcctgttt tcaagcctca tgaattttat gagcatattt cttgactttt tccaaatttt
    18421 gtttgtgaaa attctaactt tatggtatta taataacatg aaaatgaaat gtaagggctt
    18481 caatttagat gattatttga tattacaact tattttctat aagagaaagg agttggtagc
    18541 ttgactcttc gttcacattc tgaacggcta atggggacta ctatcactat ttcattagtg
    18601 gatgagcaag ccgatatctt tctccaaaaa tcctttgact tgctcaaaga acttgaatac
    18661 cgcttcaacg ccaatagtca agaatctgag ttgatggaaa tcaattatca agcaggaata
    18721 gcccccgtca cggttcatcc agacctgttt gagctgattt cacttgggtt agagcatagc
    18781 ctagcgctct ctagccacct caatatcagc attggtccct taattcaaac ctggcgtatc
    18841 ggtttttcag atgccaaggt cgcccagcct caagaaattg aatcggtgct gcctctaatc
    18901 aatcctcatg gtatcgagtt agattcttct acttctactg tgtttttaaa acagaaagga
    18961 atgaagatcg atctaggttg tttagccaaa ggatacagtg cggataaggt tgcccaattt
    19021 cttaggaaag agggggtgac ttctgccttg atcaatctgg gagggaatat cctgaccatt
    19081 ggaaaaaatc aggcaagagg ggataaccca tggcaaatcg ggattcaaga cccagccaat
    19141 cctaggggaa atcatttaat gaccatccct gttgtcaata aatctgtcgt gacttcaggc
    19201 atttatgaac gtcacctgac cgtcgatgga caagattacc atcacatttt tgacagtcaa
    19261 acaggatatc ctgttgaaac ggaactagcg agtctaacaa tcatctctga taaatcagtc
    19321 gatggcgaaa tctggacgac tcgactcttt ggagaaagac ctgcttctat cctctggcaa
    19381 gtcgaaagtt tggagggcat cgaagtcatc ctcatcgata aagaaggcca cctaagctgt
    19441 tcttcaggaa ttcctactct atagagacaa ctgtttcaat ggaattttaa aattgtatta
    19501 tgtaaaaaga gaaaggaaat ctcatgttaa aacttattgc tattgttgga acaaattcaa
    19561 aacgttctac aaaccgtcaa ttgcttcaat acatgcaaaa acacttttct gacaaagctg
    19621 aaattgagct tgttgaaatc aaggccattc ctgtcttcaa caaaccagct gacaagcaag
    19681 tacctgctga aatattggaa attgctgcta aaatcgaaga ggcagatggc gttattatcg
    19741 gtactcctga gtatgatcac tctattccag ctgttttgat gagcgctctt gcttggttgt
    19801 cttatggtat ttacccactt ttgaacaaac caatcatgat tacaggtgct tcttacggta
    19861 cgcttggttc atctcgtgcc caattgcaac ttcgtcaaat cttgaatgct cctgaaatca
    19921 aggcaaatgt tcttccagat gaattcttgc tctcacactc tcttcaagca tttaacccaa
    19981 gtggcgactt ggttgacctt gatgttatca agaaattgga tgccatcttt gatgacttcc
    20041 gtatctttgt aaaaatcaca gaaaaattac gtaatgcaca agaattactt cgcaaagatg
    20101 ctgaagactt tgactgggaa aatttgtaag ataggagacc aaaaagaatg aaatttgttg
    20161 gacttgttgg atcaaactac gatcaatcat ataaccgtaa gctcttggaa tttatccgtc
    20221 gctacttcaa actcaaattt gaattagaag ttcttgaaat tgacgaagtt ccaatgttta
    20281 accaagatga aaaatgggat gaaagtttcc aattacgtta tttatataac aaaattactc
    20341 gtgcagatgg tgttatcatt gctactcctg agcataacca cactatctca gcttcactca
    20401 aatctgtact tgaatggctt tcatacgaag ttcatccatt tgaaaacaag cctgttatga
    20461 ttgtgggtgc atcatactat gaccaaggaa catcacgtgc tcaagttcac cttcgtaaaa
    20521 tccttgatgc tccaggtgtt aatgcctaca cgcttccagg taacgaattc cttcttggta
    20581 aagctaagga agcttttgat aacaacggaa atatcaccaa cgaaggcact gttaaattcc
    20641 ttgaaacttg cttagataac tttgttaaat acgtaggagt cgtttcgaaa ttgaaaaaac
    20701 caaaaccaat cgaatcagaa gacttggatt gtggaaaacc aatcgctaca accatcacag
    20761 aagttgaccc tgacgatcca gaatgggtag aaaaagttgc agcaatcact ggtgctgttt
    20821 ctggtgatac ctatgtcaaa ttggaccacg gtatcctaac agttaaccaa attgatatgt
    20881 tcttgaaagc tatgccattt gaattgacat acgctgacga caacaaccaa ttcctctact
    20941 acaacaacgc tcaccaagat ccagacacta tgttcgctaa acgtgtacca cctcaatcag
    21001 gtagccgtat gtcgactgtt catggttctc ttccaccagc acgtatgaag aacgtagagt
    21061 gggttatcgg aacacttcgc aacggaaacc aagaatacgt ccgtacgatc gttccaggtt
    21121 ctcctgcagg tgttatcaac actcacaact accaagcaat gtactatcct gatggatcat
    21181 acgctggtat caatgaaatc gtctttaact tccaaccatg gcttgactgg tacctaaaag
    21241 agactggtca acgtttggtg ggtggtagcg gaccatttgc tcctgctgcc ggaggtcatg
    21301 gagacgccga tgctacttct ggcgcttctg attcagggga tgctggaggc cacggtggtg
    21361 acgcagacgc tacagccggt gcaagttact aatgatagtt tttaggaacc attatggttc
    21421 cttttttagc gaaaaaagac tagcaaacac aaactgctag tctctttgtt atcgtttcat
    21481 ctatctttgt taaaagaaag agtacttata ctcaatgaaa atcaaagagc aaactaggaa
    21541 gctagccgca ggctgtactt aagtacggta aggtgaagct gacgtggttt gaatttgatt
    21601 ttcgaagagt attattatga atttgggtca tcaagactac ggccatgtaa tcctttttca
    21661 cgttgtactt ggcgtagttt ttctggtgta acatcgtttc cttcttcgtc cacaattttg
    21721 attccttcaa tgtggtgacg aacagcgcgg cgataacctt cgatgtactc ctcacgtagt
    21781 ttggcttgtt ccactttttc ttctgatgtt aagccttctg tttttttctt tttagcaagc
    21841 tcattgatac gagcaatttt tttcggatcc atttcttctc ctttgtaata agataaagtc
    21901 cgtttatcaa attttattta gtgttaattt ggttaaaaca agcaaactta gctgctttct
    21961 tggttagttg tgacaagatt gccaaacgat tttgacggac agcctgatct tcagccatta
    22021 ccatagtatt ttcaaagaaa gcatcaatga ctgggctaag cgcaaagagt tgtttcaatt
    22081 gctgacttgc aggtcctgat aaaacgagtg tttctactgc ttctgccaaa gctttttctt
    22141 ggtcattctc aaatagtgct gaatcaactg tagcaacccc ttctgccttc tcagccaggt
    22201 taaaggcacg agaaagtgat tcaacagatg gtttaaaatc ttcttccttg cttacttcta
    22261 cgagagcact tgctgcttcc aacatatctg ccacaacaaa gtttgaacct gcaagaactg
    22321 cttccttgat atcttttgga gtagagccca tcatcttatc aacacgagcc ttgataaagt
    22381 ccataacctc tgctttattt tcataagtca aactgtcaaa tttcaatgca taaaggctat
    22441 caatcagctc atccatagca atgtgccaac caaaggcatc caagatacga accacacctt
    22501 gagttgcacg acgaagggca taagggtcat tagaacctga tggaatcaat cctactgaga
    22561 agaaactcaa aatcgtatcc aatttgtctg caatggctag aactgcgccg accttgctct
    22621 ctggaagttc tccttcagct gatgtaggca tgtagtgttc acgaatagca gctgccaccg
    22681 ctggagtttc accagcaaga agggtgtatt tttcacccat aattccttgg agttcgtcaa
    22741 attcaccaac catacctgtc aacaagtcaa acttgtaaat ggctgctgca cgagcaaggt
    22801 caactgtttc atccactgac aaaccagctt tttctgccaa aagtacagtg atttgacccg
    22861 tacgaatcat gtgttcacga agggaaccaa tcttctcatg gaaggtgaca ttgtttaatt
    22921 tttcaacaag atctgaaatc accaattttt ggtcttcacg ccagaagaat tctccgtctt
    22981 ccaagcgggc taccaagact ttttcatttc ctttgatgac atttttcaaa cgctctgcgt
    23041 ttccgttacg aacagaaatg aagtttggca agagttttcc atcttgatca cgaacaacaa
    23101 agtaacgctg gtgttccttc atagaagtca ccaagacttc ttctggaact tcaaggtatt
    23161 tagcatcaaa acttcccatg aaggcagttg ggtattcaac caaattcaag acttcattca
    23221 gcaaatccgc atcaatttcg atacgtacac catgttttgc ctcaatttcc ttgatttggt
    23281 caacaatcat ttgctcacgt tcacatggat ctgcgattac aaactgctta cgaaggtctt
    23341 cttcatagct caatgctgac tgaattttgg tttctttccc caagaaacga tggccacggc
    23401 tcacacgact tcccttgata tcaaggaaat ccaagtcaaa ctcttgctca tccaagagaa
    23461 cagttaaagt gtgaacaggg cggatgtatt caaagctatt tccagcccag tgcatgctga
    23521 cagggaaagt caatgacttc aagacatcta caacaccagg aacaatggct tcaactgctt
    23581 gaccaacttc ttccttagtg acatagacat attcttcacc cttgatttca cggaattcga
    23641 tatcttcaac agtcaaacct ttcccacgga caaatccttg agctgctttg gtgaagtttc
    23701 catcactatc caaggcaatt ttctttgctg gtcccttgaa atcttctgtc aaatcagact
    23761 gtttgtctga taaaccagtc acacgaacag ccaaacgacg tggtgttgag aaggtttgaa
    23821 tggcttcaaa agacaggcgg ttttccttga ggaaggctgc cattttttcg cctagttgtt
    23881 tttcacttgg tgtgacaaca taggctggta attcttcaag accgagttct actaataagt
    23941 tttttgtcat gttttttctc cgaaaagtat ctttttattt tccagtttgc cttatgtgat
    24001 tggcacgcaa gcaaccaact tcgttgtttc attgctaaaa ttggagccaa aagtctccaa
    24061 ttttgcctag tatccttagt ttcactaagg ataccttcat cactgcggca actggctcag
    24121 ggctcactct gttcgcccat tttcgtaatt tgtcactctc tttattctgc gtcttctgct
    24181 aggagtttag ctcttgtttc ttcatccaaa agtgggtagc ctaggcgttt gcgttctgcg
    24241 acaaaggttt tggctacgac acgggccaag ttacggatac gagcgatata gcctgcacgc
    24301 tctgttacag atacggcacc acgcgcgtca agcagattaa aggtatgtga acatttgaga
    24361 acatagtcat aggcagggtg taccaagcct tcttctaatg cacgaccagc ttctttttca
    24421 aacttatcaa aattttcaag caacatttct tggtccgaaa tttcaaatga atattttgag
    24481 tgctcatact caggctggat aaagatttct ccgtatttta caccatcagc ccactcgata
    24541 tcatagacag agtcaacttc ttgaatgtaa gaagccaaac gctccaaacc ataggtaact
    24601 tccgcagtca caggaccagt tgccaatcca ccgacttgtt ggaaataagt aaactgagtg
    24661 atttccatcc catcaagcca aacttcccaa ccaagaccag ctgaaccagt cgatggattt
    24721 tcccagttgt cctcaacgaa acgaatatcg tgttccaaag gattgatacc caatttttcc
    24781 aaagactcaa ggtaaagctc ttggatattt gatggagaag gtttcataac cacttggaat
    24841 tggtggtgtt ggtaaaggcg gttagggttt tccccataac gaccgtcagc aggacgacgt
    24901 gatggctcta cataagccgc attccatggc tcaggtccga tagcacgaag gaaagtgtaa
    24961 ggactcattg ttccagcacc tttttcgttg tcataagcct gcataagcat acaaccttgg
    25021 tcattccaaa attgttgcaa agtaaggatg atttcctgga aagtcaattt tttag
//
LOCUS       DAVMEI010000014        23262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVMEI010000014 DAVMEI010000000
VERSION     DAVMEI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.014"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..356)
                     /locus_tag="VYQ24_000466"
     CDS             complement(<1..356)
                     /locus_tag="VYQ24_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967648.1"
                     /translation="MIDLYLSKNNHRNQLLLEFFQNYGIEVSCHSISEMTRDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMA"
     gene            complement(349..798)
                     /locus_tag="VYQ24_000467"
     CDS             complement(349..798)
                     /locus_tag="VYQ24_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967649.1"
                     /translation="MKALNKESILYCDELETELHDAEMMQLDEQIFLMPNYPCEFEVT
                     FLDYYHKKHNYPLFYESYLQNIMEFLESQDIKNGADAFIDDNHNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKSINFSNLLDSLIVSEYQMEPNLWEVSHD"
     gene            complement(782..2140)
                     /gene="dcm"
                     /locus_tag="VYQ24_000468"
     CDS             complement(782..2140)
                     /gene="dcm"
                     /locus_tag="VYQ24_000468"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEV1967650.1"
                     /translation="MRFIDLFSGIGGFRLGMESVGHECIGFCEIDKFARKSYKSIFQT
                     EGEIEFHDIRDVSDAEFKKLRGKVDIICGGFPCQAFSIAGRRLGFEDTRGTLFFEIAR
                     AAKQIQPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQLLNSKDFGVPQNRE
                     RVFIIGHSRKRGTRLLFPFRREGQTTNSETLKTLGNLNPSKSGMSGKVYYSEGLAPTL
                     VRGKGEGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRHGIVVVGDLPTSFK
                     ETGRVYGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLE
                     RPSSQYRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYHVAGVLIDGQFYRLRIRR
                     ITPKECFRLQGFPDWAFEAARKVSSNSQLYKQAGNSVTVPVIAAIAKKLKEIEEKDES
                     AK"
     gene            complement(2239..3018)
                     /locus_tag="VYQ24_000469"
     CDS             complement(2239..3018)
                     /locus_tag="VYQ24_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1967651.1"
                     /translation="MKRITANQYQTSERYYKLPKILFEDEKYMDMKLEVKVAYSILKD
                     RLELSLSRGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKILKEYDLIDEVQQSSS
                     EKGRLANKIYLGELSSTPVGNSNRPSVKKRLGQVENETAPVSHSAPRETEVSETKYSE
                     TDSLFIEDEEDRNTQPILKRKVEKVTKYDRDYIWGLVQDQFRREGFSETASEIAMTDF
                     ERIYQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGGG"
     gene            complement(3015..3188)
                     /locus_tag="VYQ24_000470"
     CDS             complement(3015..3188)
                     /locus_tag="VYQ24_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967652.1"
                     /translation="MTKELQSSRYIVISFLVREMGIDIVEAISLMAELEKSGLVRLES
                     SGDLTLKELGGAL"
     gene            complement(3397..4758)
                     /gene="rlmD"
                     /locus_tag="VYQ24_000471"
     CDS             complement(3397..4758)
                     /gene="rlmD"
                     /locus_tag="VYQ24_000471"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1967653.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLFRRFDLKPYDEKEHSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVECVVLLQR
                     KKG"
     gene            complement(4832..5806)
                     /locus_tag="VYQ24_000472"
     CDS             complement(4832..5806)
                     /locus_tag="VYQ24_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1967654.1"
                     /translation="MRKKLFLTGAAVLWAVTAMNSAHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(5880..6494)
                     /gene="pmp23"
                     /locus_tag="VYQ24_000473"
     CDS             complement(5880..6494)
                     /gene="pmp23"
                     /locus_tag="VYQ24_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1967655.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(6494..7468)
                     /locus_tag="VYQ24_000474"
     CDS             complement(6494..7468)
                     /locus_tag="VYQ24_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1967656.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(7476..8732)
                     /locus_tag="VYQ24_000475"
     CDS             complement(7476..8732)
                     /locus_tag="VYQ24_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1967657.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(8794..9225)
                     /locus_tag="VYQ24_000476"
     CDS             complement(8794..9225)
                     /locus_tag="VYQ24_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967658.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYIHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(9227..9829)
                     /locus_tag="VYQ24_000477"
     CDS             complement(9227..9829)
                     /locus_tag="VYQ24_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1967659.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(9813..10652)
                     /gene="prmC"
                     /locus_tag="VYQ24_000478"
     CDS             complement(9813..10652)
                     /gene="prmC"
                     /locus_tag="VYQ24_000478"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1967660.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(10652..11731)
                     /gene="prfA"
                     /locus_tag="VYQ24_000479"
     CDS             complement(10652..11731)
                     /gene="prfA"
                     /locus_tag="VYQ24_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1967661.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(11728..12318)
                     /locus_tag="VYQ24_000480"
     CDS             complement(11728..12318)
                     /locus_tag="VYQ24_000480"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1967662.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            12441..12623
                     /locus_tag="VYQ24_000481"
     CDS             12441..12623
                     /locus_tag="VYQ24_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1967663.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            12759..14132
                     /gene="mnmE"
                     /locus_tag="VYQ24_000482"
     CDS             12759..14132
                     /gene="mnmE"
                     /locus_tag="VYQ24_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1967664.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIVQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEQLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(14179..15114)
                     /gene="dapA"
                     /locus_tag="VYQ24_000483"
     CDS             complement(14179..15114)
                     /gene="dapA"
                     /locus_tag="VYQ24_000483"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1967665.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(15170..16246)
                     /locus_tag="VYQ24_000484"
     CDS             complement(15170..16246)
                     /locus_tag="VYQ24_000484"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1967666.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(16832..16904)
                     /locus_tag="VYQ24_000485"
     tRNA            complement(16832..16904)
                     /locus_tag="VYQ24_000485"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16869..16871),aa:Thr,seq:ggt)
     gene            complement(17004..17513)
                     /locus_tag="VYQ24_000486"
     CDS             complement(17004..17513)
                     /locus_tag="VYQ24_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1967667.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(17511..17607)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            17636..18067
                     /locus_tag="VYQ24_000487"
     CDS             17636..18067
                     /locus_tag="VYQ24_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1967668.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            18149..18526
                     /gene="mscL"
                     /locus_tag="VYQ24_000488"
     CDS             18149..18526
                     /gene="mscL"
                     /locus_tag="VYQ24_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1967669.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(18568..19662)
                     /gene="hemH"
                     /locus_tag="VYQ24_000489"
     CDS             complement(18568..19662)
                     /gene="hemH"
                     /locus_tag="VYQ24_000489"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1967670.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            19817..21040
                     /gene="pepT"
                     /locus_tag="VYQ24_000490"
     CDS             19817..21040
                     /gene="pepT"
                     /locus_tag="VYQ24_000490"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1967671.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKEQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(21076..21705)
                     /locus_tag="VYQ24_000491"
     CDS             complement(21076..21705)
                     /locus_tag="VYQ24_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967672.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            21670..21891
                     /locus_tag="VYQ24_000492"
     CDS             21670..21891
                     /locus_tag="VYQ24_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967673.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(22000..22476)
                     /locus_tag="VYQ24_000493"
     CDS             complement(22000..22476)
                     /locus_tag="VYQ24_000493"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1967674.1"
                     /translation="MTPNNGVSAADDGYVFNPNDIVRDTGDAYIVRHGDHYHYIPKSS
                     LNNPPSHSNTEEAGSSSSSVLSNPSLHVHHEEEDGHGFDANRIISEDSEGFVIPHGDH
                     NHYIKVQTKGYEAALKNKIPSLQSNYQPGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(22516..22656)
                     /locus_tag="VYQ24_000494"
     CDS             complement(22516..22656)
                     /locus_tag="VYQ24_000494"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1967675.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAA
                     KV"
     gene            complement(22700..23260)
                     /locus_tag="VYQ24_000495"
                     /pseudo
     CDS             complement(22700..23260)
                     /locus_tag="VYQ24_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6733 a         4925 c         4291 g         7313 t
ORIGIN      
        1 gccatgtaaa tcaattgttt cgtatctctt ggtaataaag ccctagcttc ctccaaattt
       61 agatttggat aaactcgctt atttgaaacc gcaagaggaa gtctgatggt tagttcagga
      121 ttttttaata tcatttcaat gaaatccgtt agtcttaaat tgtcacggtt cttaaatcgt
      181 aataaattag gagataaaaa ctcaaaacaa tctgaagaat agctcatcat ctcaattaat
      241 ttatcccttg tcatttcaga aatcgaatga caggatacct caatgccata gttttggaag
      301 aattctaaaa gaagttgatt tctatggtta tttttactta gatagagatc aatcatgtga
      361 gacctcccaa agattcggtt ccatttgata ttctgagacg attaaggaat ctaataaatt
      421 ggagaagtta attgatttct tgtcttcatc ataagctttt acagttactt gggttgtaag
      481 tattccctct tttccctcgg ctcgatagcc ttgcccatat aaaacaaaaa caagattatg
      541 attatcatct ataaaggcat cagctccgtt ctttatatcc tgactttcaa ggaattccat
      601 aatgttttga agataggatt cgtaaaatag ggggtaattg tgttttttat ggtaataatc
      661 taaaaatgtc acctcaaact cacatggata attgggcatc aaaaatattt gttcatccaa
      721 ctgcatcatt tctgcatcat gtaattctgt ttctaattca tcacaatata gtattgactc
      781 tttatttagc gctttcatct ttttcctcta tttcttttaa tttttttgcg attgcggcaa
      841 tcacaggaac agttacacta ttaccagctt gtttatagag ctgactatta ctagagactt
      901 ttctagcagc ttcaaaagcc caatcaggaa agccctgcag tcgaaaacac tctttaggag
      961 tgattcgtcg tattctcaaa cggtaaaatt gtccatctat taaaacacca gctacatggt
     1021 aaacttgttt atcttctcct tcatagctag ccactactac tcccatttga ccactagttg
     1081 ttaatgtatt cgctatacct tttccaactc taccacgacg atactgagaa cttggtcttt
     1141 ctaaattgat tgaatctcca atctctgctt gagcataccc ttttttagtt gcttcccgga
     1201 cttttagaaa ttggattggt tctggaatca gtattttggg aattttatct cctccttgca
     1261 tcgtagtcag tgttggagat aagccctcgc ttccatagac tcgacctgtc tccttaaagc
     1321 tagtcggtaa atctccaaca acgacaatac catgacgatc ctgagtattt aaagtaaaca
     1381 tcggctcttg gttttcctta aagcgtctcc cattttgtct cttgtctaat ctgtctggtg
     1441 tcatacaagg aatcgcaacc ttgaatcctt ctccttttcc acgaactaag gttggtgcaa
     1501 gaccttctga ataatagact ttaccgctca ttccactttt tgatggattc aaattcccta
     1561 gtgtttttaa agtctcagag ttagttgttt gaccttctcg tctgaaagga aataagagtc
     1621 tggtacctct ctttctagaa tgtccgataa taaacaccct ctctctgttt tggggaacgc
     1681 caaaatcctt actgttaagc aactgccact caacatcaaa ccccaactca tcaagtgtgg
     1741 taaggattgt ggtgaacgtc cgtcccttat cgtgattgag taggccttta acattttcaa
     1801 gaaaaagaaa acgtggttgg atttgtttgg ccgcccgagc aatttcaaag aacaaagttc
     1861 ctctagtatc ttcaaatccc aatcgtcttc ctgcgattga aaatgcttga caagggaatc
     1921 ccccacagat gatatcgact ttccctctaa gttttttaaa ttctgcatct gaaacatctc
     1981 gtatgtcatg aaattctatt tctccttccg tttgaaaaat ggacttataa gatttcctag
     2041 caaatttatc aatctcacaa aatccaatac actcgtgtcc aacactttcc attccaagtc
     2101 gaaatccacc tatacctgaa aataaatcaa taaatctcat ttcttgatca cctctttctt
     2161 atcgactacc attgtcataa ctgtcactcc acttgaaatg acaataagca taactaatat
     2221 ccactcgatt gacgacattt aaccacctcc ttttctaaca cggttattcc aaacagaata
     2281 caggccatta aacacaaatt cagcaagtac ttccgctcgt ctaacaaagc gaacattgtc
     2341 aagagcatac tgatagattc tttcaaaatc agtcatagca atttcactgg ctgtttcaga
     2401 aaaaccttct cgtctaaatt gatcttgtac caatccccaa atataatctc gatcatattt
     2461 tgtgaccttt tctacttttc ttttcaaaat aggttgagta ttcctatcct cctcatcctc
     2521 aataaataaa gaatcagtct cactatattt agtctcacta acttcagtct ctctaggggc
     2581 tgaatgtgag actggggccg tttcattttc aacctgccct agtctttttt taacactagg
     2641 cctgtttgaa ttacctactg gggtagaaga taattcccct aaataaatct tattagctag
     2701 tcttcctttc tcacttgaag actgttgaac ttcatcaatt aagtcatatt ctttaagaat
     2761 ttttttgata gacagtaatt ttgacttcga gcaacctaac agcttcatca gtttagaatt
     2821 agaaaatact aaataaaccg ctccttcttc atctatccaa ccacgactga gagataattc
     2881 taaacgatct tttaaaatag aataagccac ctttacttct agtttcatat ccatatattt
     2941 ctcatcctca aaaagaattt taggtaattt gtaataccgt tctgaagttt ggtattgatt
     3001 tgcggtaatt cgtttcatag cgcacctcca agttctttga gtgttaaatc tccgcttgat
     3061 tccaatcgaa ccaagccact tttttctaat tcagccataa gagagatggc ttcaacaata
     3121 tctattccca tttcacgtac taaaaatgaa atgacaatat agcgtgatga ttgtaattct
     3181 tttgtcattt tccctcctga aaataaaaaa aggagaacaa aatacaattg ttctctcttt
     3241 agataaatat ttattgttgt attcattttt ccaatgcgca aacgacatct acaaatacat
     3301 atacatctat gtgccttttt aggacttttt tgacgtcaaa attgccttta tctacaaaca
     3361 caaaatgctt gtaaaatagc ttaaaatcaa ggattttcac cctttttttc gttgtagcaa
     3421 cactacacac tccacgtgat gcgtttgagg aaatagatcc accggctgga ctttcttcaa
     3481 ttcatatccc aactcttggt atagtttaat atcacgcgcc atggttgcga cattgcagga
     3541 gatataggcg atgcgatcgg ctcctgtttg ggcgcttgct ttgataaagc tttctgtcaa
     3601 gcccttgcgt ggaggatcaa ccaagataac ggttggttga atcccatctt taagccaatt
     3661 cttcatagca ttttcagctg tgtcacagac atagtgggcg tttgaaatat tgttcagctg
     3721 agcatttttt ttactattct caaccgcttc tggaatcagt tcaacaccgt agacttcttt
     3781 gacatgcttg gcgactgata aaccaatggt tccaatacca gaataagcat caataaccac
     3841 gtcatctttt tttaactctg caaagtcaat ggctgtttga tagagtttct ccgccatttc
     3901 agtattgact tggtaaaagg ctgggccagc gatttggaag tcatttccca acatctggtc
     3961 cgtaatatag tcttgaccat aaagagtgcg ccactcctta ccaaaaatcg cattggtatt
     4021 ctggtcgttg atattttgca tgacagacac aatctctggg aactgcttga taacttgttc
     4081 aatcaattgg tcaacacgaa aaacttttgg acgagttgtc accaaaacga ccatgatttg
     4141 ccctgaatag tgaccacgac gcaccacaag attccgaatc aatccagaat gttccttttc
     4201 gtcataaggt tttaaatcaa aacgacggaa caggtctcga agagctacta cgacttggtc
     4261 aatgacagga tcctggataa agaaatcttc aaggggcatg aggttgtgcg aattcttacg
     4321 gaaaaatcct gtttccaaga caccattcac tcgacgaacg ggcacctgcg ccttattgcg
     4381 atacttgact ggatgttcca taccaagcgt ttcagcaact tctacatctg caattccagc
     4441 aatcttgtag agactgtcct tgacttgctt ggttttaaac ttgagctgtt ctggataaga
     4501 aaggtgtcct aaatccgcga ttcctgaacg caggtaagcc aaatctagat cttgattacg
     4561 gtgtggtgac tggacaaggt atttttcaac ttttccaaag ccaatctttt tattgacctt
     4621 gaggacacgc atgagaattt tttcactcgg taaagcattc tctacaaaaa agaccaaacc
     4681 atctaccttg gcaactcctg ccccttcatg ggtcaaatca acaatttcaa cttctacaat
     4741 atcatttttc tttaacattc ttttctcttt ctattggccg acaaaaaaga cggcaacgtt
     4801 acggttacca tccattatac catgaaattt cttaagaacc aaaactcttg aagaagttga
     4861 caagggcttg ccagagggag cttaagaagt taccaccgtc ctctagcgcc ttatccgcat
     4921 caaagttaag gttgatattt ttaaaactgt cgccagcttg tgatacgatg ctttgtttaa
     4981 ggtcatttag ggttttagtg aaatctgcat tgctgaggat atcactcttt gagagattca
     5041 aggcaaaatt gatgatgata ttgatctggt ttcctgttat gacctgatca agtttgtaat
     5101 tttttaaggt atcttcaaca atcttgcgta catcttcttc tgtcagattt cccttgcttt
     5161 ctttagcttt ggcgagtcct gacttgatat cagctagggc aacgtttaat ttattagcat
     5221 catagcctga tttgtccttg ttttcagcat tgatatctga caaagccttt agctcttctt
     5281 gagccaaatc tttattggct tgcggcacct tggctccatt agcttctagc gaatagtaga
     5341 tcccagccaa agcactctca cctgtaactg gaataggggc tgctacagtg attttggcat
     5401 gttccatacc cagcgttact gctgcgtttc ggtacatatc ctgagtcacc ttagtgatat
     5461 tttctggtgt ttcaatcttg acctcaagtg gcgatttgtc acctagcttt tgaatcttgg
     5521 ctgatgaata caactgtaag ctagagtcat tggccacatt catgatttta gaataaacat
     5581 caggtgtcat ggtcttgagt tctttggtat ctgttgaggc attgtagccc agttttttaa
     5641 gagtttgatt tttttggtct tcagataggg aggaacctag gacatattca ggttggacat
     5701 aggtttcatc gataactttt tgaacatctg ttgctgcatg ggcgctattc atagctgtta
     5761 ctgcccacaa gaccgcagca ccggtcagaa agagtttctt tctcataggg aatttcctcc
     5821 tttacttttc tagagtaata tatctatctt aaagaaaact tataacaaaa acacctggtc
     5881 tagccagatg ttgaaaagag agtgaaacat ttgatgatgt aaaggttaag tcgtacctgt
     5941 ctagaataat aatagtttcc tccatttaca tagagttcag caccgtgaaa aatggaaatg
     6001 gggtgaatat aactataagt ctttccagtc ctattaccaa gcaagggggc aacagtctca
     6061 cgagagtact gtttggctag agccaaggta ttttccttgc cattttgggc gataaaatcg
     6121 atataggcag gtccaaaatt ataggcttga acagctgtcc agatatctac acccttcttc
     6181 tgcgccagat agagattgcc tgtcagagtt tgaatgcctt gccgaatgct agaggcatta
     6241 tcattgatgg tgttggtgga accacttgca gactcactag actgcataac atcgccttct
     6301 tttccttttg tttcagtata aatcatagca agcacaagct cttcgtttgc tggggtgtct
     6361 ttttcactca atatttctcg caccatgggt tgataggtca tgacttgttt gacatcttga
     6421 tgaacgcggt aagctttata gccagcaaaa aggaagactg ctagtacaag cactcttcga
     6481 attcgtttaa acattattta ctttggatat cctcgatatt tttgattaag atagagtagg
     6541 ttccattttc gttttggata aactcaacag actcggcgtc ttgatagacg ttattgggaa
     6601 cgatgagctc aattccattt gataaggaga gtttttggtt ttcaaatttc tttaattggc
     6661 gactggcatc aatttcatca aattgaacag gttctggtac ggcttctttg acttggtcaa
     6721 taaagctcaa acgagccgtc agattgttgt caaaaaggtc attagccaat ttctcaggtg
     6781 acaattcatt gctttcttct aggttgttga aaatagctga tttgaccttg gattgaaatt
     6841 gaaaatcatc tgtgttaaaa gattcagcaa ttctctgggc tgttttttcc agttccttga
     6901 tagatttttt aggagaaatc ttaggagcga cagcaagaag attatctgaa aaatagttca
     6961 aaaaagtccc gttgtacttg attcgttttt caatcaggtg atacttgcga ctctgaagat
     7021 tgaccaccaa ggcctcgtca gcacccgttc caaatccagg caggttattc tgagtcagct
     7081 tgattggatt atcaacttct cctccgaggt gggtcaaggt ctcccgcagg gcaattcgca
     7141 agaaagcgaa atgttctaca ccttctttag aaaattgaac aaaaatcaag tcattggtct
     7201 tgagattttc agaaatgcta aactcctctt tccagagatt agccagcgtt actgatgtct
     7261 ccaacaaatc gtctgtaata tgattgaaga agggattttc ttcttcgaaa atcccagtct
     7321 tggcttcatc tgaatacaca tgttcaattt ttttacgtag gtattcttcg atttttggag
     7381 taatattgag aaacttatct gctaagaaca gctcggtatc atccggactg aactggtgaa
     7441 taatggcttt cttaatataa atatccataa aagttttagt cctcgtataa tgggaaggca
     7501 tctgtcaatt ctttgactgc acttctcact tcttctaata cagcctcatt ttctgaattc
     7561 ttaagggttt taatgatgag ttcagccact ttgcgacttt cttcttcacc aaatccacgt
     7621 gcagtaatag ctgctgctcc gatacgaatc ccacttgtct tgaatggcga caaggtttcg
     7681 taagggattg aatttttatt tagggtaata ttgacttcat ccagcaagtt ttgagcaact
     7741 tttccgtttt ctacaacctt agtcacatca acaaggaaga gatggttttc agttccacct
     7801 gaaataatac ggaaatcagg gtcttgcaag aagacatctg ccatagcctt gctgttctta
     7861 attacattgg cagcatattc cttgaaggct ggatccaaaa cttctttgaa ggaaactgcc
     7921 ttagccgcca caacatgctc taaaggaccg ccctgaatac ctgggaaaat agctgaattg
     7981 atttttttag caagttcttc gtcattggtc aaaatcaaac caccacgagg tccacgaagg
     8041 gttttgtggg tcgttgttgt tgtgatatga gcgtatggaa ctgggcttgg atgaaggcca
     8101 gccgcaacca aaccagcgat atgggccatg tccaccatga gcttcgcacc gacagcatct
     8161 gcgatttcac ggaattttga aaaatcgata atttgagaat atgctgaagc accagctaca
     8221 atcagttttg gttttacttc ttgggcttgt ttcaagatag catcaaagtc taagagttcc
     8281 gtttcaggat caacactata agaaacaaag ttgtaggttt gaccagagaa gctaacagga
     8341 gccccatgag tcaaatgacc acctgatgcc aaatccattc ccataaccgt atcacctggc
     8401 tcaatcaagg acatgtaagc cgcacagtta gcttggcttc ctgaatgtgg ttggacattg
     8461 gcaaatttag caccgaaaat ttcttttgcg cgttcaatag caagagtctc tacaacgtct
     8521 actacatcag ttccaccata ataacggcgt cctgggtaac cctcggcata tttatttgtc
     8581 aagatagacc cttgagctgc cataacagcc ttggaaacta cgttttccga agcaattaac
     8641 tcgatattat tttgttggcg ttcttcttct ttggcaatag cattccagag atcagcatca
     8701 tatgctttaa aatcatcttt gtcaaaaatc ataggtcttc tcctttattg tgtgactagt
     8761 ccattagttt gattttacaa taagaaaatc aaactaaaag atgcgaataa accgtttctg
     8821 cattttatca caagtatagc caactttttc ataaaatgca tgagcaccca gacgatgatt
     8881 ggcagaattt aagcggataa acccataacc acatcttttt gcttcttctt ccaacccttg
     8941 tagtaaactt ttaccaatac cttgaccttg cgcttgaggt gaaactgcta aagctaagat
     9001 attaaatcct gctttggaat agagtgattc gtaaacttca gcgtggatat atccaagtaa
     9061 gacatgatta gctgcatcct catagccaag taggaaatga tgggaatcct gagacagtct
     9121 agctagttgg ctagccgttt cctctggact aaaagtataa cccaaagcct cttggttgat
     9181 gtcacatatc gctttcacat ctgtttcttg caaatctctt agcatttcaa gcctcctcaa
     9241 aagaaatctc tggcaaccga gcaagaatat cttctcgttt aattgcgcct tggcgtaaga
     9301 ttttcacctt gtctccagac aaatccacaa tagttgaatc ctgtccagtt agaaaagcat
     9361 cgtcttccag acccagaacc tcttggtcaa aatccttcag aatttgttca aaggttacac
     9421 cacttgcctg acctgagata ttggcagacg gcccaatcaa gggacctgtc tctcgaatta
     9481 aatccagtgt gatagggtga ctgggcatcc gaaatccaat agttgcaagg tcagaattta
     9541 cccaataggg aactcggtca ttggcttcga gaataatggt caagggacct ggcaaaaagg
     9601 tctctacaag tttttgtaga taagttggct gattcttaga aaagtgcaag atatcctcga
     9661 aagaggcgat attgagattg agtgccttat ctctaggacg acgtttgagt tggtaaacat
     9721 ggtcaactgc tttttcatct aaggccttgg caaaaagacc ataaacagtc tctgtaggta
     9781 gaacgacagc tccaccattt tccaactctt gtctaatcct gtccatcatc aaccacaacc
     9841 atcctatttt gaccaaattg gtctttgagt gttcgaactt ttttttcagg aagatgtttt
     9901 ctaaaaagtt cgggaacact ttgaccttgc ttgtatccaa tttcaaggta aatcttacca
     9961 ccatccttga gatagtcttt tgcatcttcc gcaattctac agtaaatagc taggccatcc
    10021 tcgtctgcaa agagagctag atgaggctcc gaatgcaaaa cattcaaacc tacctctgac
    10081 tcatcttcac gagagatata gggtggattg gatacaatta tatcatattt ttcagaaatt
    10141 tctgcaaaac aatcagattt tttaaaaaat atattaagat tttgatcgct agcattctct
    10201 gatgcaagct ctaaggcctc ttgagaaaca tctgctgctg tcactgacca atctggtcta
    10261 ttttttgcta gagcgagagc aatagctcca ctacctgttc caatatccag aactgagaga
    10321 ttcgtctcaa gattttcagt tagaataagc tccactaact cctctgtttc tggacgagga
    10381 atcaaaaccc gttcatccac ctttaaatgc attccataaa aatctgcctg tccaatgatg
    10441 tactgagctg gcttgtgagc tgctagttgc tggtaaatat cttctacaaa ttgtttttct
    10501 tcctctgttg tcacctcctg ctggagggca aaaataaagt ctgtaaaaga tagatttttc
    10561 agactacgat agacaaaaga gaggctttcc gcttcctctc cttgtcttat caactcttct
    10621 tcaaaatttg aaaataattg agctaatttc attatttgtt taattcttct agtttttgtg
    10681 tttggtcata aagcaccaag gcatccacaa cttcgtccaa tttaccagac aaaatcgtat
    10741 ctagtttttg gagggtcaag ccgatacggt ggtctgtgac acggttttgt gggaagttat
    10801 aagttcggat ccgttctgaa cggtcaccag taccgattgt cgacttacgc tcagcgtctt
    10861 gttcatcctg agcaatctga gcaaagtggt cagcgacgcg tgcacggata atcttcatag
    10921 ccttctcgcg gttcttctgc tgggtacgtt cttcctgcat ctcaaccttg atattggttg
    10981 gcaagtgaac gatacgaacg gcagtcgcaa ccttattgac gttctgtcca ccagcaccag
    11041 aggcgtgata gatgtcgaca cgaaggtctt ttggatcaat gtcgtattca acctcttcaa
    11101 cttctggcat aacaagaact gtcgctgtcg aagtatgaac acggccttgg ctttctgtca
    11161 caggaacacg ttgcacacgg tgggcacctg attcatactt aagcttagag tatacagact
    11221 gacctgaaac catagcaacc acttctttaa aaccaccgac accattcata gaggcttcca
    11281 tgacttcaaa gcgccaacct tgggcttccg catacttttg gtacatagtt agcaaatctc
    11341 cagcgaaaag tgccgcttcg tctccaccag ctgctccacg gatttcaagg atgatattct
    11401 tgtcatcgtt tggatccttt ggaaggagca aaattttcag tttttcttca tattcttctt
    11461 tttcagcctt ggcatctttg agttcttgct tggccatttc ttccaagtcc gcatctccgc
    11521 ctgattcctt aatcatctct tcggcatcga cgatattttg aaggacttgt ttatactcac
    11581 ggtaggctat tacggtgtca cgattggaag cttcttcttt tgaaagctcc ataaaacgct
    11641 tggtgtctga aacgacatca gggtcactca gcaattctcc tagttcttca taacggtctt
    11701 ctacagcttg tagttgatca tagatgttca tttttcttca ttctccttat tgatttcagg
    11761 ggcaaaataa tgtttacggc aaaccgagat ataggtttca ttaccaccaa tctggatctg
    11821 ttctccatcg taaacgggca gtccatcctg tgttcgcaac accatggtcg cctttttctt
    11881 gcaatactga cagatggtct tgatttcgtc aatcttgtct gctaaaagca agagatattt
    11941 ggaaccttcg aacaattcat tgcgaaagtc atttttcaag ccaaaagcca tgacgggtat
    12001 gtctaactcg tccacaacac gagctaggtc ataaacatgg tggcgtttga gaaactgggc
    12061 ttcatcgacc aaaacacagt aaggtttttc tggtaggtct cggatatagc caaagatatc
    12121 cgttgtttcc tcaatcgcaa gggcagggcg tttcatgcca attcgactcg acacatagcc
    12181 aacaccgtca cgcgtatcca gagccgaggt cataatcaca acaccttttc cttgctcctc
    12241 gtagttatag gccactttga gaatctcaat cgttttacca gagttcatgg tcccataacg
    12301 atagtacaac tgtgccatgt ttcttgcttc acgtccattt ctaaattttt gctacattct
    12361 agtatatcat aattttctta agctttaaac ggcaaaatgt ggtaaaatgg aagaaatcaa
    12421 aactagtgga ggaagctatt atgccatttg tacgcatcga tttatttgaa ggacgcacgc
    12481 tcgagcaaaa gaaagctctt gctaaggaag taacggaagc ggttgttcgc aacactggag
    12541 cacctcaatc tgctgttcat gtcatcatca acgacatgcc agaaggaact tatttcccac
    12601 aaggggaaat gcgcaccaaa taagctagct taagcagaat tgcttaggct ttttcaatct
    12661 ccaagtagca cccattgaag aaatagtcta aatttgttac aatttgaaaa gagacttgga
    12721 aaattccaag aaaagagcaa ttaattaaag gaaacattat gattacacgt gaatttgata
    12781 ccatcgctgc tatctctact ccactaggtg aaggggctat tggtattgtc cgcctgagcg
    12841 gaacagacag ttttgctatt gtgcaaaaga tttttaaagg aaaagacttg aacaaggttg
    12901 ccagccacac tctcaactac ggtcacatta ttgatcctct gactggtaaa gtcatggacg
    12961 aggttatggt tggggctatg aagtctccaa agaccttcac tcgtgaggat attatcgaga
    13021 ttaacaccca cggtgggatt gcggtgacca atgaaattct ccagctagct attcgtgaag
    13081 gggctcggtt ggcagaacct ggtgaattta ccaaacgtgc ttttttaaac ggtcgcgtag
    13141 acttgacaca ggcagaggct gtgatggata tcatccgtgc caagactgac aaggccatga
    13201 acattgcggt caaacaatta gacggctccc tttctgacct cattaacaat acccgtcaag
    13261 aaatcctcaa tacacttgcc caagttgagg tcaatatcga ctatcctgaa tatgatgatg
    13321 ttgaggaagc tactactgcc gttgtccgtg agaagactat ggagtttgag caattgctaa
    13381 ccaatcttct tagaacagcc cgtcgcggca aaatccttcg tgaaggaatt tcaacagcta
    13441 tcatcggacg tcccaacgtt gggaaatcca gccttctcaa caacctcttg cgtgaggaca
    13501 aggctatcgt aacagatatc gctgggacaa cacgagatgt catcgaagag tacgtcaaca
    13561 tcaatggtgt acctctcaaa ttgattgata cagccggtat tcgtgaaaca gatgacatag
    13621 tggaacaaat cggtgttgag cgttcgaaaa aagccctcaa ggaagccgac ttggttctac
    13681 tagtgctaaa tgccagtgaa caactgactg cgcaagacag acaacttctt gaaattagcc
    13741 aagataccaa tcgcattatt ctacttaata aaaccgacct gccagaaacg attgaaactt
    13801 cgaaactacc tgaagacgtt atccgtattt cagtccttaa aaaccaaaac atcgacaaga
    13861 ttgaagagcg aatcaacaac ctcttctttg aaaatgctgg cttggtcgag caagatgcta
    13921 cttacttgtc aaacgcccgt cacatttccc tgattgaaaa agcagttgaa agcctacaag
    13981 ccgttaatca aggtcttgag ctggggatgc cagttgattt gcttcaagtt gacttgactc
    14041 gtacttggga aatcctcgga gaaatcactg gggatgctgc tccagatgaa ctcatcaccc
    14101 aactctttag ccaattctgt ttaggaaaat aagaaaaatc catgatcctt cattcggtca
    14161 tggattttat tgtctttatt agtaatctgg tcttaagacc cctgttacag ttgccttagt
    14221 tgcttcgtag tcgccatcta cgacaacctt gataatgcgt ttggcatctt cttctggtgc
    14281 tggaacaaga ggtagacgag tgggtccagc ttcaaatccc atatagttaa gaattgcctt
    14341 aactggagca ggacttggat aagagaagag agcattaacc ttaggaatga atttacgctg
    14401 aattgctgcg gctttcttca tatcgctttc tgcaatggca gtaaacatct cgtgcatttc
    14461 atccccattt gtatgagagg caacagaaat aaccccatcc gccccaaggt tcatggcatg
    14521 gaaagcatct ccatcctcac ctgtataaat caagaactct tcaggcttgt gctcaatcaa
    14581 gtaagccata ttagccaagc tagtacattc tttgacaccg ataatatttg gatggtcagc
    14641 caagcgaagc atggtttctg gagtcaattc gaccactaca cgccctggaa tgttatagat
    14701 aataattggt aggtcagaag catctgcaat agccttaaag tgctgataca tcccttcttg
    14761 agaaggtttg ttgtagtaag gaacaatagc aagcccagct gcgaaaccac caaattccgc
    14821 tacttctttg acaaactcaa tagagtcacg cgtatcatta gtacctacac ccgcaatcaa
    14881 aggaacgcgt ccattgacaa ccttttgtac agccgcaaac aactccaact cctcatcgtg
    14941 ggtcaaagtt ggactctcag cagtcgttcc tgcgagaaga attccatccg tatgatgggc
    15001 caataaatgc tcaatcaagg ctggaatagc atcaaagtta atggaaccat cctcatggaa
    15061 gggggtaata aaggctgtaa tgattttaca ttcttttaaa tcttgataag acacgaaaac
    15121 acctctctat ttcaaagaag gagttcatct gaactcctaa acgatatgac tattttaatt
    15181 caaatttcaa ttcagctgtt ggacgaacca atccacgttc atgaagagtt tcagcaatct
    15241 gaactgagtt ccaagcagca cctttgagaa ggttatctga aacaacccac atgtgaattc
    15301 ctttttctgc atccaagtct ttacggatac gaccaacaaa ggtatcacgt gaaccaactg
    15361 cattgatggc ttgaggatag atttgatgaa ctacatcatc ttcaagaaca gcacctggga
    15421 aggatgcgat agctgctttg acttcttcaa ttggagccac ttcctttgtt tcaatataaa
    15481 ctgactcaga gtgagctgac aagactggaa tacgcacaca tgtcgcagat actgcaatgc
    15541 tatcatcttc cataattttc ttagtttcct tggtcatctt catctcttcg tacgtgtaat
    15601 cattatcagt gaagacatca atttgtggaa gagcgttaaa ggcgatagga taatgtttct
    15661 tgtcaccacc tgaaggcaag atttccgcat gcaaatcacg tggtttcact ccatcattca
    15721 agacctcacg aagttcacgt tgtgtctcaa gaattgctcc cataccagca cctgaaacgg
    15781 cttgataagt tgaaacaatg atacggtcca agccccattt ttggcgaacc ggctcaagag
    15841 ccaccatcat ttggattgtt gaacagttag ggcaggcaat gattccgttg tgggcatcaa
    15901 gtgcatgagc attgacctct ggaacaacca aaggaacatc tggattttga cggaaataag
    15961 atgtattatc cactactacc acgccagctt ttactgcgta tggtgcatac ttagctgatg
    16021 tcgaaccacc tgctgaaaag agagcaatat caactccttc aaaagctgtt tcagtcgttt
    16081 cttcaatcgt aatatcttga tctttaaatt tcaatgactt acctgctgaa cgtgcagaag
    16141 caaggtaacg gattttatcg attggaagtg ttgattcttc caacattttt atcatctgag
    16201 caccgacagc acctgtcgcg ccgactacag caactgtata tcccataaat aacctctttc
    16261 ggaattttct aaaaatttct ataatagaag tattatacta ctttttccat gaattgcaaa
    16321 gctttttcat cattttttgg aaaataaaaa tccccaatcg ctagactagg gattaagagg
    16381 catcttcatg tgatgagcag gttcacacaa ctcatcaagg tccgctcctg cgttatgacc
    16441 tccttatgct caatagtagc ccgaagacta cctatcgact catcgcatct actattctac
    16501 taaagagaat gacttttgtc aacagacgaa actctttata ctcttcgaaa atctcttcaa
    16561 acttcgtcag cgtcgccttg ccgtatgtat ggttactgac ttcgtcagtt tcatctacaa
    16621 cctcaaaaac atgttttaag ctgacttcgt cagttctatc tacaacctca aagcagtgct
    16681 ttgagcaacc tgcggctagt ttcctagttt actctttgat tttcattgag tattagtttt
    16741 atttacacag gtgcataaga aatatattgg aaaaattggg ggcagacata acaaaaaccc
    16801 ttgtaaatca agggcttttc tgtctattta atgctaattg ccgggattga accggcgacc
    16861 tcatccttac catggatgcg ctctgccaac tgagctaaat cagcttactt aagaagtata
    16921 ctatattcag aaaaacttgt caagtttcct tagaaatttt ccataggaaa aagccaggta
    16981 agaactacct agcttatctt cttctattta cttaaatcaa ttcgacgacc aattttacca
    17041 ataataatcg ctccaataat caatgaagca aattcaccga ttcctgttgt aaaccaagta
    17101 aggaagaatg gtaattctgc tacaatattt aactctgcag cgatagtaaa catggaaatt
    17161 gaaaagagga ttgaaaagaa gaaatgatct tttcgaatca atccgttaaa gaggtaatcc
    17221 ttgctatatt ttgcaaaaag ccaaacacct aaactgagga atactaaggt tgaaccacca
    17281 ccaacaaaga catctagcag tccgaagcta aagaaattag caatcataca accgatggta
    17341 actccgatga tgtacttagg attgtaaaaa gccataaagt tcatcatttc tgaaatacga
    17401 aactgataag caccatagct gatggcattt aggggcggtg tgacagtcaa aacgacgtag
    17461 atagcagcga cgattgcaat atctgcaaca tcacgaatag ttaatttttt catcttttct
    17521 cctttggcgg ttatccgcgt gtaatatgct tggtgaaaga agctaagcac caagggttga
    17581 ttcattcaac cttactagta tagcacaata gcctgcttta tgctatactt aaagcatgaa
    17641 aaaacctatt caaaaatttt ttaacaatga aatcttagcc tatctctttt ttggccttgc
    17701 gacaactcta gtttcgattc tctcacgact agtcatttat caactaagtc atcaggaact
    17761 ccttgctacg gccttggcaa atattatcgg tatcttattt gcctttatca caaatgatac
    17821 gattgtattt aagcaaaaaa agaaaaatag acttatccgt ttagtgaaat tttctcttgc
    17881 tcgcctttct acttttctgt tagacttgtt tttaactttt ctctttgtaa cccaatttcc
    17941 tcacatcata gggcaattcg taactgaaaa cattgataag ataaatgcta ttgaaacaat
    18001 aattgcacaa atattcatta ttatcatcaa ttatatcttg agcaaggtct atatttttag
    18061 aaaataatgc tccatttatg agccttttaa ttgccttcta taatgattta ggatacaatg
    18121 aaacacgaaa cattgaaagg aaaacagtat gttaaaaaat ctaaaatcgt tcttgcttcg
    18181 aggaaatgtt attgaccttg ctgtcggtgt tgtcattgcc tctgcttttg gtgctatcgt
    18241 tacttcactt gtaaacgaca ttatcactcc tcttatttta aatcccgctt tgaaagctgc
    18301 taaagtcgaa cgtatcgctc aactttcttg gcatggagtc ggctatggta actttttaag
    18361 tgctattatc aattttatct ttgtgggtac cgccctcttc tttattatca agggcattga
    18421 aaaagcacag aaactgactg gcataaagaa agaaaaaact gccgaaaaaa aaccaaccga
    18481 attggaagtc cttcaagaaa taaaagctct ccttgagaaa aaataatcga ttaaaaggat
    18541 ctagcgcaaa gctaaatcct tttttcttta ctctttgggt aacttgcctg ctttttcaag
    18601 catttttttg ataagataag gcatcttaac attctcccgc ccttttttct caatcagttt
    18661 tttcacaaat tctggcattt gtaaatcttc agattcagct aaaatgtttt tcgtctcgtc
    18721 tgaaggaact aactcatcaa aggtctcttc ttctgggaga aattccttaa actcttgatt
    18781 ttcattgact ctaactgtat tgaccaaagc atcaatcaaa cgagaatctg tatttggcat
    18841 tggtggacga tggtagttta ccccaaattc ctggcataag tcataacatt ctacatcatt
    18901 gtcaaacaag acctcaatat gctcactgat gaaactaata ggtacaaaaa tataatggtc
    18961 tggatgttct gtctgttctc tgagatactc caaaacatct ggtttaatcc atggaatacc
    19021 aatatcactc tcactctgcc aagtgttggt atattgttct gaactcaagc ccagtttttc
    19081 agcgactagc ttgctatttt cgaaaatttg gtctatataa ggatcaccaa agtccaaggc
    19141 aaaaatgggc acactatggg ctgaaaagat gactttaaag ctgtcttgct ttacttcttc
    19201 ttttaaaatc ttagcaattt catctgccca atagtttaag agcgcttctt cctgatacca
    19261 gtccttaatg accaaaaatt gaatttgttt gctttctaaa aatttttcat agcccatgac
    19321 agagtagaaa gaataatggg gctccaaaat caagcaaata cactgttcaa ttccgtctgc
    19381 ttccatttga ccgatcacat ctggaataaa gggactggaa aacttattag caaagtagac
    19441 actgtattca ttccccagcc tagcttctac caaggcaacc tcttgacggg taattttttg
    19501 cagaggcgtg cctccaattc gtacataatt atcatagaga gtctgaatct cgtggtcttg
    19561 gggtctcacc ccacgacgaa tatttgtgaa aaaatcagct acaccttcaa aggtaatctc
    19621 ttctggtgaa ccgaaggtca tcattaaaat tgcttttttc atgactgctt ccttgtgatg
    19681 tttttatcaa gtttatttta tcattaatta atcgataagt aaacgctaat atagggacat
    19741 tctcaatctt ctgtcttttg tttttctctt ctttctatga tacaatggaa aaaataaatt
    19801 caaaaggagt ttttttatga cttatccaaa tctcttggac cgcttcttaa cctacgttaa
    19861 ggtcaacacg cgctctgatg aacactctac tactactcca agtacacaga gtcaggttga
    19921 cttcgcaaca aatgtcctaa ttcctgaaat gaaacgtgtt ggactgcaaa atgtttacta
    19981 tctaccgaat ggttttgcta ttggaacctt gccagccaac gatccgtctt taacacgtaa
    20041 gattggtttt atatcgcaca tggatactgc tgattttaat gctgaaggag tcaatccaca
    20101 ggtaattgaa aactacgatg gtggtgtgat tgaactaggg aattctggtt tcaaactcga
    20161 tccagctgac ttcaagagtc ttgaaaaata tccaggacaa acgctcatca caacagatgg
    20221 aacaagcttg ctaggtgctg atgacaagtc aggaattgct gaaattatga cagccattga
    20281 atatctaact gctcatcctg aaattaagca ctgtgagatt cgtgttggtt ttggtccaga
    20341 tgaagaaatc ggtgttggtg ccaataaatt tgatgcagaa gattttgatg tggattttgc
    20401 ctacactgtt gatggtggtc cactaggtga acttcagtac gagactttct cagccgctgg
    20461 tgctgaattg catttccaag gccgtaatgt ccaccctggt actgccaaag agcagatggt
    20521 caatgccctt cagctagcaa ttgattttca taatcaactt ccagaaaatg accgacctga
    20581 gttaactgaa ggttaccaag gtttttacca tctaatggat gtgacaggta gtgttgagga
    20641 ggtgcgtgca agctacatca ttcgtgattt tgaaaaagat gcctttgaag cgcgtaaagc
    20701 atccatgcaa tctatcgctg ataagatgaa tgcagaactt gggagctacc gtgtcactct
    20761 caacttgaca gaccagtact acaatatgaa agaagtcatt gaaaaagata tgactccaat
    20821 taccattgct aaagccgtta tggaagatct aggtatcacg cctattatcg aaccaatccg
    20881 gggtggaaca gacggctcta agatttcctt tatgggaatc ccaactccga atatctttgc
    20941 aggtggcgaa aatatgcacg gacgttttga atacgttagc cttcagacta tggaacgtgc
    21001 agttgatacc atcattggca tcgtagctta taaaggctaa aaagacgagg tagctcagct
    21061 acttcgcctt tctttttatt ctactggttt ttcttgattt ccagtagttg tagaagattc
    21121 tgttgtttca ttttctgaag ttgattcagc aggtttagaa tctcttgtat tgcttggttt
    21181 gttttcgtcg ctagcaattt caatgttaga ttctgcagtt gcgtttggtt ggttctcagc
    21241 actggtgtta tcactatttg cttcagcatt tcttgctgga cttgtttctt cacttgcgct
    21301 agcttttgac tggatttgat gattcaaaac tagaatagct tttgtcgatt caagtaaagc
    21361 tgttttgtct ttactcttag cagaaagttg atctaataat gcatccacct tatcaaagtc
    21421 cgcatcagat ccattattac tttctaaata agagtgaagc gacatgagaa tatcgtagag
    21481 tttttgatag agtacaagtg tctgagaatc ttgctcagca ttttcctttt cttgttgaag
    21541 ggcgctagcg atacgagtca agacatcttt tacctgactg tttacttcat ccaagtctgc
    21601 atcagccttg tttgtggcag cttttagatt ttctacttct tctgccaagg attgtctgat
    21661 tccttcttca tggatttgtt ccaagagttg atttaccttg ctcaaaagac tttctacttc
    21721 ttccttgcta tctgtcgcag attattggtt gccatctacc atgtactcct aaaacaggag
    21781 agttataatc caagattaca aggccttaca gaaataagaa atccagataa gacaatgttc
    21841 gtccaagacg ctattcgctt cgcacagcag cacggattca atatgcttta attttaaagt
    21901 ttaggtgtca agacctcttt ttagtgtgcc caaaatttag agaagtaatc aatcaactaa
    21961 cttttatttt tttcaaactt tcagtaaact gacctaaagc taactcaatc tgtctttgta
    22021 gatgcttctg ctatcagcta gaagttgatc tacttttgcc aagactgcct tctcatcaaa
    22081 agttccaggt tgatagttgg attgcaggga tggaatcttg tttttcaaag ccgcttcata
    22141 tcccttagtt tgaaccttga tgtagtgatt gtggtcgcca tgaggaatca caaaaccttc
    22201 tgaatcttca cttataattc gattggcatc aaaaccatga ccatcttctt cctcatggtg
    22261 gacatgtagt gacggattac ttaatacaga actagaagaa cttcctgcct cttccgtgtt
    22321 agagtgtgat gggggattgt taagagatga cttaggaata taatgatagt gatccccgtg
    22381 tcttactata taagcatcac ctgtatctct gacaatatca ttagggttaa agacataacc
    22441 atcatctgct gcagaaacac cattattcgg tgtcaccgac aaagattgac tgagagctgt
    22501 agtattccct gataattata cttttgcagc tgctaattca cctgccgaca agtcactctc
    22561 aggaatgaaa tgatagtgac caccatgtgg tactatagta ggttgaaata gaatatgagc
    22621 aaattgataa ggggatttta aagtaatttc taacaatgat ttagaaacta tgatgtgcta
    22681 ttctaaattc aactcactat atataaccat catcggtagt ataacgtccc tgtaattttg
    22741 ctacagatac ttctgcacta gctcctttat cgtctttacc atgttcttgt ttttggcgat
    22801 tgatttcatc ttttgttcgt acattttctg catgagcttg atctttaagg taaacataat
    22861 actttccatc taccttaata atatatcctc ccttaaccta actgacgata tcttgatctt
    22921 tcggctgata gttgggggct ttcattaata gctcttcact aaagagcgca tcaaaaggaa
    22981 ctttaccatt atagtagcga taatgatcgc catgagaagt tacataacct tgatctgtaa
    23041 tcttaataac aatttgtttt gcttgaattc cttctttttg actaacctag tctggagtca
    23101 aattttcagt cttcttagtg tctttattac tgtttacata tgaaacacga tttttatctg
    23161 tattggcctg ttagctatgt tggttcagag tataaacaca cagacttaag gaaaggataa
    23221 caacagatcc agctgctata tatttctttt taaatttcat aa
//
LOCUS       DAVMEI010000015        22861 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVMEI010000015 DAVMEI010000000
VERSION     DAVMEI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.015"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            337..2490
                     /locus_tag="VYQ24_000496"
     CDS             337..2490
                     /locus_tag="VYQ24_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1967676.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKEQKNGLFSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2503..3852
                     /locus_tag="VYQ24_000497"
     CDS             2503..3852
                     /locus_tag="VYQ24_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1967677.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQGGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4022..4729
                     /locus_tag="VYQ24_000498"
     CDS             4022..4729
                     /locus_tag="VYQ24_000498"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1967678.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4786..8511
                     /locus_tag="VYQ24_000499"
     CDS             4786..8511
                     /locus_tag="VYQ24_000499"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1967679.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLSIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGVIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLSELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLSYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8604..10046
                     /locus_tag="VYQ24_000500"
     CDS             8604..10046
                     /locus_tag="VYQ24_000500"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1967680.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FYDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10083..11105
                     /locus_tag="VYQ24_000501"
     CDS             10083..11105
                     /locus_tag="VYQ24_000501"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1967681.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11102..11647
                     /gene="purN"
                     /locus_tag="VYQ24_000502"
     CDS             11102..11647
                     /gene="purN"
                     /locus_tag="VYQ24_000502"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1967682.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11731..12240
                     /locus_tag="VYQ24_000503"
     CDS             11731..12240
                     /locus_tag="VYQ24_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1967683.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12265..13812
                     /gene="purH"
                     /locus_tag="VYQ24_000504"
     CDS             12265..13812
                     /gene="purH"
                     /locus_tag="VYQ24_000504"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1967684.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     NDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            13934..15196
                     /gene="purD"
                     /locus_tag="VYQ24_000505"
     CDS             13934..15196
                     /gene="purD"
                     /locus_tag="VYQ24_000505"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1967685.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTVATVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15599..16087
                     /gene="purE"
                     /locus_tag="VYQ24_000506"
     CDS             15599..16087
                     /gene="purE"
                     /locus_tag="VYQ24_000506"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1967686.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16074..17165
                     /gene="purK"
                     /locus_tag="VYQ24_000507"
     CDS             16074..17165
                     /gene="purK"
                     /locus_tag="VYQ24_000507"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1967687.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAKVTVAPYKVVTSSQDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVQA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAQLPAIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17175..17402
                     /locus_tag="VYQ24_000508"
     CDS             17175..17402
                     /locus_tag="VYQ24_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1967688.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17465..18763
                     /gene="purB"
                     /locus_tag="VYQ24_000509"
     CDS             17465..18763
                     /gene="purB"
                     /locus_tag="VYQ24_000509"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1967689.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18818..22744)
                     /gene="strH"
                     /locus_tag="VYQ24_000510"
     CDS             complement(18818..22744)
                     /gene="strH"
                     /locus_tag="VYQ24_000510"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1967690.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKEEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETETGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDKAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEAY
                     PLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPAEESK
                     ALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
BASE COUNT         6173 a         4581 c         5485 g         6622 t
ORIGIN      
        1 atttcggttt tgttaacttg taattcttat attttctagg tagaacgttt gttctgccta
       61 tttttttatc caaaaagtgc agttgggagg gagataggct catttgggaa ggaagtccag
      121 tttttgttta gtgattgggg taagatagtt gttatcagat gagtttatac tcttcgaaaa
      181 tcaaattcaa accacgtcaa cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc
      241 tatctacaac ctcaaaacag tgttttgagc agcctgcggc tagtttccta gtttgctctt
      301 tgattttcat tgagtattag ggaaaaggag atgaatatga aatttgggaa acgtcactat
      361 cgtccgcaag tggatcagat ggactgcggt gtagcttcat tagccatggt ttttggctac
      421 tatggtagtt attatttttt ggctcacttg cgagaattgg ctaagacgac catggatggg
      481 acgacggctt tgggcttggt caaggtggca gaggagattg gttttgagac gcgagccatt
      541 aaggcggata tgacgctttt tgacttgccg gatttgactt ttccttttgt tgcccatgtg
      601 cttaaggaag ggaaattgct ccactactat gtggtgactg ggcaggataa ggatagcatt
      661 catattgccg acccagatcc tggggtgaaa ttgactaaac tgccacgtga gcgttttgcg
      721 gaagaatgga caggagtgac tctttttatg gcacctagtc cagactacaa gcctcataag
      781 gaacaaaaaa atggtctgtt ctcttttatc cctatattag tgaagcagcg tggcttgatt
      841 gccaatatcg ttttggcaac actcttggta accgtgatta acattgtggg ttcttattat
      901 ctgcagtcta tcattgatac ctatgtgcca gatcagatgc gttcgacact agggattatt
      961 tctattgggc tagtcatcgt ctacatcctc cagcaaatct tgtcttacgc tcaggagtat
     1021 ctcttgcttg ttttggggca acgcttgtcg attgacgtga ttttgtccta tatcaagcat
     1081 gtttttcacc tccctatgtc cttctttgcg acacgcagga caggggagat cgtgtctcgt
     1141 tttacagatg ctaacagtat catcgatgcg ctggcttcga ccatcctttc gattttccta
     1201 gatgtgtcaa cggttgtcat tatttccctt gttctatttt cacaaaatac caatctcttt
     1261 ttcatgactt tattggcgct tcctatctac acagtgatta tctttgcctt tatgaagccg
     1321 tttgaaaaga tgaatcggga taccatggaa gccaatgcgg ttctgtcttc ttctatcatt
     1381 gaggacatca acggtattga gactatcaag tccttgacca gtgaaagtca gcgttaccaa
     1441 aaaattgaca aggaatttgt ggattatctg aagaaatcct ttacctatag tcgagcagag
     1501 agtcagcaaa aggctctgaa aaaggttgcc catctcttgc ttaatgtcgg cattctctgg
     1561 atgggggctg ttctggtcat ggatggcaag atgagtttgg ggcagttgat tacctataat
     1621 accttgctgg tttactttac caatcctttg gaaaatatca tcaatctgca aaccaagctt
     1681 cagacagcgc aggttgccaa taaccgtcta aatgaagtgt atctagtagc ttctgagttt
     1741 gaggagaaga aaacagttga ggatttgagc ttgatgaagg gagatatgac cttcaagcag
     1801 gttcattaca agtatggcta tggtcgagac gtcttgtcgg atatcaattt aaccgttccc
     1861 caagggtcta aggtggcttt tgtggggatt tcagggtcag gtaagacgac tttggccaag
     1921 atgatggtta atttttacga cccaagtcaa ggggagatta gtttgggtag tgtcaatctc
     1981 aatcagattg ataaaaaagc cctgcgccag tacatcaact atctgcctca acagccctat
     2041 gtctttaacg gaacgatttt ggagaatctt cttttgggag ccaaggaggg gacgacacag
     2101 gaagatatct tacgggcggt cgaattggca gagattcgag aggatatcga gcgcatgcca
     2161 ctgaattatc agacagaatt gacttcggat ggggcaggga tttcaggtgg tcaacgtcag
     2221 agaatcgctt tggcgcgtgc tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg
     2281 actagcagtt tggatatttt gacagagaag cggattgtcg ataatctcat ggctttggac
     2341 aagaccttga ttttcattgc tcaccgcttg actattgctg agcggacaga gaaggtggtt
     2401 gtcttggatc agggcaagat tgtcgaagaa ggaaagcatg ctgatttgct tgcacaggga
     2461 ggcttttacg cccatttggt caatagctag aaagaggaga ggatgaaacc agaattttta
     2521 gaaagtgcgg agttttataa tcgtcgttac cataattttt ccagtagtgt gattgtaccc
     2581 atggcccttc tgctcgtgtt tttacttggc tttgcaactg ttgcagagaa ggagatgagt
     2641 ttgtccacta gagctactgt cgaacctagt cgtatccttg caaatatcca gtcaactagc
     2701 aacaatcgta ttcttgtcaa tcatttggaa gaaaataagc tggttaagaa gggggatctt
     2761 ttggttcaat accaagaagg ggcagagggt gtccaagcgg agtcctatgc cagtcagttg
     2821 gacatgctaa aggatcaaaa aaagcaattg gagtatctgc aaaagagcct gcaaggaggg
     2881 gagaaccact ttccagagga ggataagttt ggctaccaag ccacctttcg cgactacatc
     2941 agtcaagcag gcagtcttag ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag
     3001 aatgcagcag ctagccaaac ccaagccgaa atcggcaacc tcatcagtca aacagaggct
     3061 aaaattcgcg attaccagac agctaagtca gctattgaaa caggtgcttc cttggccggt
     3121 cagaatctag cctactctct ttaccagtcc tacaagtctc agggcgaaga aaatccccaa
     3181 actaaggttc aggcagttgc acaggttgaa gcacagattt ctcagttaga atctagtctt
     3241 gctacttacc gtgtccagta tgcaggttca ggtacccagc aagcctatgc gtcagggtta
     3301 agcagtcaat tggaatccct taaatcccaa cacttggcaa aggttggtca ggaattgacc
     3361 cttctagccc agaaaatttt ggaggcagag tcaggtaaga aggtacaggg aaatctttta
     3421 gacaagggga aagttacggc gagtgaggat ggggtgcttc atcttaatcc tgagaccagt
     3481 gattctagca tggttgcaga aggtgcccta ctagcccaac tttatccatc tttggaaaga
     3541 gaagggaaag ccaaactcac agcttatcta agttcaaaag atgtagcaag aatcaaggtc
     3601 ggtgattctg ttcgctatac tacgactcat gatgccggga atcaactttt cctagattct
     3661 actattacaa gtattgatgc gacagctact aagactgaga aagggaattt ctttaaaatc
     3721 gaggcggaga ctaatctaac ttcggagcag gctgaaaaac ttaggtatgg ggtggaaggc
     3781 cgtttacaga tgattacggg caagaaaagt tatctacgtt attatttgga tcaatttttg
     3841 aacaaagagt aatgttcgtg tttttagagt taaataattt ttaaactgtg agaaagattc
     3901 ttcttgcagt tttttcttta caatttttga aaagcatctg ctatttattc ggttaaattc
     3961 ttgtgttttt tggttttttg tggtaaaatg tgctcaagta atacgaaagg cgaactttaa
     4021 aatgtcaaaa caattgatct attcgggaaa agctaaagat atctatacaa ctgaggatga
     4081 aaatcttatt atttcaactt acaaggacca ggcgactgct ttcaacggtg tcaagaagga
     4141 gcagattgca ggtaagggag tcttgaataa tcagatctca tcttttattt ttgagaaatt
     4201 aaatgcggct ggtgtggcga ctcactttgt ggagaaactt tcagacacgg aacaactcaa
     4261 taaaaaggtt aagattattc ctttggaagt cgtgctccgc aactatactg ctggttcctt
     4321 ttcaaaacgt tttggtgtgg atgagggaat cgccttggag actccgattg tcgaatttta
     4381 ctataaaaat gatgatttgg atgatccatt tatcaatgat gagcatgtga aattcctaca
     4441 gattgcggat gaccagcaga ttgcctactt gaaggaagaa acgcgtcgta tcaatgaact
     4501 attgaaagtc tggtttgctg agattgggct taaattgatt gactttaagc tagagttcgg
     4561 ttttgacaag gatggcaaga ttatcttggc agacgaattt tcaccagata actgccgctt
     4621 gtgggatgct gatggcaacc acatggataa ggatgttttc cgtagaggat tgggagaact
     4681 aaccgacgtt tacgaaattg tctgggaaaa gttgcagggt ttaaaataac aacctcaagg
     4741 ctgtttggga atattgcaag agctgaaata aaggaataag aattgatgga taaacgtatt
     4801 tttgttgaaa aaaaggctga ttttcaggtc aagtcagaga gtttggttag agagctccag
     4861 cacaacttgg gactgtcaag cttgaaaagt attcgtattg tgcaagtata tgatgtattt
     4921 gacttggttg aggacttgtt tgcacctgca gagaagcaca ttttctctga gcaggtaacc
     4981 gaccatgttt tagatgaagt atctgtgcag gcggatcttg ctaactatgc tttctttgcc
     5041 attgaaagtc taccagggca gtttgaccag cgtgcagctt cgtcacagga agccttgctt
     5101 ttgttgggaa gttcgagtga cgtgacagtc aacacagccc aactttactt ggtgaataaa
     5161 gatattgatg cgactgagtt ggaagctgtc aaaaactacc tgctcaatcc agttgattct
     5221 cgtttcaagg atatcacgat agggattgcc aagcaggagt tttcagagtc agacaagacc
     5281 attccaaaat tgcctttctt tgaaagctat acagcagaag actttgctcg ctacaaggcc
     5341 gaacaaggga tggccatgga agtggatgat ttgctctcta tccaagacta ctttaagtca
     5401 atcgggcgcg tgccaactga gactgaactc aaggttttgg acacttactg gtctgaccac
     5461 tgccgtcata cgacttttga gacagagttg aaacacatcg acttttcagc ttctaaattt
     5521 caaaagcaat tgcagtcaac ctatgacaag tatattgcca tgcgcgagga attaggtcgg
     5581 tctgaaaaac cacaaacctt gatggatatg gcgactattt tcggtcgtta tgagcgtgct
     5641 aatggacgat tggatgatat ggaagtctct gacgaaatca atgcctgctc agttgaaatt
     5701 gaagtggacg ttgatggtgt caaggaacct tggctcctca tgtttaaaaa cgaaacccac
     5761 aaccatccaa cagaaattga gccatttggt ggagcggcta cctgtatcgg tggagttatt
     5821 cgtgatccgt tgtcaggccg ttcctatgtt taccaagcca tgcgtatttc aggtgctggt
     5881 gatattacag caccgatttc ggaaactcgt gctgggaaat tgccacaaca agtcatttct
     5941 aaaacagcaa ctcatggtta ttcttcatat ggtaaccaga ttgggcttgc cacaacctac
     6001 gttcgtgaat acttccaccc aggctttgta gctaaacgta tggaacttgg tgccgttgtt
     6061 ggtgcggctc ccaagggcaa tgttgtccgt gaaaaacctg aagcaggtga tgtgatcatc
     6121 cttctcggag gtaaaacagg tcgtgatggt gtcggtggtg cgacgggctc ttctaaggtt
     6181 caaacagttg agtctgtaga gactgctggt gctgaggttc aaaaaggaaa tgccatcgaa
     6241 gaacgcaaga ttcagcgcct cttccgtaat ggcaatgtca ctcgtctgat caagaagtcc
     6301 aatgactttg gggcaggcgg tgtctgtgtg gctatcggtg aattggcaga cggtcttgaa
     6361 atcgacctca acaaggtgcc tcttaaatac cagggcttga atggtacaga aattgccatc
     6421 tctgaatcac aagaacggat ggcggtcgtg gttcgtcctg aagatgtgga taccttcgtt
     6481 gccgaatgta acaaagaaaa tattgatgct gttgtggtgg cgacagtaac tgaaaaacca
     6541 aatcttgtca tgcactggaa tggtgagaca atcgttgact tggagcgtcg tttccttgac
     6601 accaatggtg tgcgcgtggt tgtcgatgcc aaagttgtgg acaaggatgt caaactccca
     6661 gaagagcgtc aaacatctgc tgaaacactg gaatcagata cccttacggt tctatctgac
     6721 ctcaaccatg caagtcaaaa aggattacag actatctttg actgctctgt tggacgctca
     6781 acggttaatc acccacttgg tggccgttac caactcacac caactgaggc atctgtgcag
     6841 aaattgccag ttcaacacgg tgtgactcat actgcgtcgg tcattgctca aggtttcaac
     6901 ccatatgtag ctgaatggtc tccataccac ggtgctgctt atgcggttat cgaagcaacc
     6961 gctcgtttgg ttgctgctgg tgccaactgg tccaaggctc gtttctctta ccaagagtat
     7021 ttcgagcgta tggataaaca agcagagcgt ttcggtcagc cagtagctgc tcttctaggt
     7081 tctattgaag cacaaattca gcttggcttg ccatctatcg gtggtaagga ctccatgtct
     7141 ggtacctttg aagaattgac cgttccgcca accttggttg cctttggggt gacgacggca
     7201 gatagccgta aggtgctctc tccagaattt aaagctgttg gggaaaatat ctactacatc
     7261 ccaggtcaag ccctctctgc agagattgat tttgacttga ttaagaaaaa ttttgctcag
     7321 tttgaagcca tccaagctga ccataaagtg acatctgcat cagctgtcaa atacggtggt
     7381 gtagttgaaa gtttggctct tgctaccttt ggaaactata ttggtgcaga ggtgaccttg
     7441 tctgaactta aaacagcttt gacagctcaa ttaggcggct ttgtcttcac atctcctgaa
     7501 gaaattgctg gagtagagaa gattggacaa acgaaagcag actttacact gactgtcaac
     7561 ggtgtgaagc tagatggaca caagcttgac agtgcatttc aagggacatt ggaagaagtt
     7621 tacccaacag aatttaccca agcaaaagaa ctagaagaag taccagctgt ggcatcagat
     7681 gttgtgatta aagccaaaga aaaggttgaa aagcctgtgg tttacatccc agtctttcca
     7741 ggaaccaact cagaatatga ttcagctaag gccttcgaaa aagaaggtgc agaggtcaat
     7801 ttggtgccat tcgtgacctt gaatgaagaa gctattgtca agtcagttga aactatggtt
     7861 gacaatatcg acaagactaa tattctcttc tttgccggtg gattctcggc tgcggatgaa
     7921 ccagatggtt cagctaagtt tatcgtcaat atcctgctta atgaaaaagt gcgtgtggct
     7981 attgatagct ttatcgcccg tggtggtttg attatcggta tttgtaatgg attccaagcc
     8041 ttagtcaaat cgggtctcct atcatacgga aactttgaag atgctaacag tactagtcca
     8101 accctcttct acaatgatgc caaccaacac gtggccaaga tggtggaaac tcgcattgcc
     8161 aataccaact caccatggtt ggctggtgtg caagtgggcg atatccacgc tattcctgtt
     8221 tcgcacggtg aagggaagtt tgtcgtgacg gctgaggaat ttgcagagct ccgtgacaat
     8281 ggacaaattt tcagccaata cgttgacttt aacggtaaac caagtatgga ttctaagtac
     8341 aatccgaatg gttctgtcca tgccatcgaa ggaattacca gcaagaatgg tcaaatcatc
     8401 ggtaagatgg gccactcaga acgttatgag gatggtcttt tccaaaatat cccaggcaat
     8461 aaagaccaac acctgttcgc atcagcggtt aaacatttca ctggaaaata agacttacag
     8521 attttctaat agatagtatc agtaatgtaa aagtcatgta aatctagctc ttgatgatta
     8581 caaatgaaaa ttaggtataa aaaatgacat acgaagtaaa atctcttaat gaagaatgtg
     8641 gtgttttcgg tatttgggga catccagatg ctgctaagtt gacctatttt ggactccaca
     8701 gtcttcaaca ccgtggtcag gagggggcag gaatcctctc caatgatcaa ggacaactga
     8761 agcgccatcg tgacatgggg cttttatcag aagttttcag aaatccagct aatttggata
     8821 aattgacagg agctggtgcg attgggcatg tgcgttatgc gactgctggc gaagcttctg
     8881 tagataacat ccagcccttc ctcttccgtt tttacgatat gcagtttggc ttggctcata
     8941 atggaaatct gaccaatgca gcctctctca agaaagaact ggaacaaaga ggagcaattt
     9001 tcagcgcgac ttcggactct gaaatcttgg ctcacctcat tcgtcgcagt cataatccta
     9061 gcctgatggg caaaatcaag gaagcgctca gccttgtcaa aggtggtttt gcctatatct
     9121 tgctgtttga ggacaagttg attgcggctc ttgacccaaa tggattccga ccgctttcga
     9181 ttggtaaaat ggctaatgga gcagtagtcg tttcctctga aacctgtgct tttgaggtca
     9241 ttggtgccga gtggattcgt gatttgaagc caggtgagat tgtgatcatt gatgacgagg
     9301 gcattcagta tgatagctat acagatgata cccagttggc gatttgttct atggagtata
     9361 tctactttgc tcgccctgat tctaatatcc acggtgtcaa tgtccatacg gcacgtaaga
     9421 gaatgggagc acaattggcg cgagaattta agcatgaggc agatattgta gttggtgtgc
     9481 ccaattcttc cctaagcgcg gctatgggat ttgcggaaga atcaggctta ccaaatgaaa
     9541 tgggtctgat caaaaaccaa tacacccagc gaacttttat ccaaccgact caagaattgc
     9601 gggagcaagg ggtgcggatg aaactgtctg ctgtttcggg tgttgtcaaa ggcaaacgtg
     9661 tggtcatggt ggatgattcc attgtacgtg gaacaacctc tcgtcgtatc gttcagctct
     9721 tgaaagaagc gggtgcgact gaggttcacg ttgccattgg aagtcctgca ctagcgtatc
     9781 catgtttcta cgggattgat atccagaccc gtcaggagct gattgcagcc aatcatacgg
     9841 tcgaagaaac tcgccaaatc attggtgcgg acagtctgac ttatctttca attgatggct
     9901 tgattgagtc gattggtatc gaaacagatg cgccgaacgg tggtctctgt gtcgcttact
     9961 ttgacggtga ctacccaacg cctctttatg actacgaaga agactatcgt agaagtttgg
    10021 aagaaaagac cagtttttac aagtaggcga cagattctcc attaaagaaa aggaaaaaac
    10081 aaatgacaaa taaaaatgca tatgcccagt cgggtgtgga tgttgaagcg ggttatgaag
    10141 ttgttgaacg gattaaaaag cacgtggccc gtacggagcg tgcaggtgtc atgggagctc
    10201 ttggtggctt tggtggtatg tttgaccttt ccaagactgg ggttaaagaa cccgtcttga
    10261 tttcagggac tgacggtgtc ggaaccaagc tcatgttggc tatcaagtac gacaagcacg
    10321 ataccatcgg gcaggactgt gtggccatgt gtgtcaacga catcattgct gcaggtgcgg
    10381 aacccctcta ttttctcgac tacgtagcga cagggaagaa tgaaccagct aagctagaac
    10441 aagtggttgc tggtgtggca gaaggttgtg tgcaggctgg tgctgccctc atcggtgggg
    10501 aaacggctga aatgccgggc atgtacggcg aagacgacta tgacttggct ggttttgcgg
    10561 tcggtgtggc tgaaaaatct caaatcattg acggttcaaa ggtggtagag ggagatgttc
    10621 ttctcggact tgcttcaagt gggattcact caaatggtta ctctttggtt cgtcgtgtct
    10681 ttgcggatta cacaggtgag gaagtcctac cagaattgga aggcaagaaa cttaaggaag
    10741 ttctacttga gccgactcgt atctatgtca aggctgtctt gccgctcatc aaagaagagt
    10801 tggtcaacgg cattgcccac atcacaggtg gtggctttat cgaaaatgtc cctcgtatgt
    10861 ttgcagatga cctagctgct gaaattgatg aaagcaaagt tccagtgctt ccaattttca
    10921 aagcccttga aaaatatggt cagattaaac acgaagaaat gtttgaaatc ttcaatatgg
    10981 gtgtgggact tatgttggcg gtcagccctg aaaatgtaga gcgtgtaaaa gaattgttgg
    11041 atgaagcagt ctatgaaatt ggtcgcatcg tcaagaaaga aaacgaaagt gtcattatca
    11101 aatgaaaaaa atagcggttt ttgcctctgg taatggctca aattttcagg tgattgccga
    11161 agaatttcca gtggagtttg tcttttcaga ccatcgtgat gcctatgtgc ttgagcgtgc
    11221 aaagcagctc ggcgttctgt cctatgcttt tgaactcaag gagtttgaga gcaagacaga
    11281 ctacgaagca gcccttgtcg aactcttgga agaacaccag attgacttgg tttgcctagc
    11341 aggctacatg aaaatcgttg gaccaacctt attgtcggct tatgaaggtc ggattgtcaa
    11401 cattcatcca gcctacttgc cagaatttcc aggagctcat gggattgagg atgcttggaa
    11461 tgctggcgtg ggtcagtctg gtgtgaccat tcactgggtg gattcgggtg tggatacagg
    11521 ccaggtcatc aaacaggttc gtgtgccacg actagctgat gataccattg acagatttga
    11581 agctcgcatc catgaagcag agtacaggtt gtatccggaa gtagtgaagg ctctatttac
    11641 agattgactt tttgatgatt catatgatat ctttgatttt aaattggagt cagtgtttgt
    11701 tgaagacggc ttcaaacgga ggtatttgta atgttagaat ctaaaaaaac aactcgatat
    11761 gtattttatg tctatctgat gttattaact tggggaatct tatttaagtt tgaaacaaat
    11821 cctgaattta tagcattttt cttagctcca aggtatatca attggattcc attttcagaa
    11881 ccactaatag tcgatggaaa aattgttttt gctgaaatgt tatttaatct gatttccttt
    11941 attccattag gtgtttgttt ccctttgata aaaactaatt tatctagttt aagaatagtc
    12001 gggacaggtt tcttgattag tttattgttt gagtgcttac agtatatttt agcaataggt
    12061 ataacagata taacggattt gactttaaat acgctaggtg tctgtgtagg cttactgatt
    12121 tatcaaattt ttataagagt gttcaaatca cagactagaa aatggatcaa tatcttaggt
    12181 atgcttagcc ttggttttgc ttatcttgtt ttactgttac tgcatttaat tggtgtttaa
    12241 ctaatgatta aaaaggagaa tataatgact aaacgcgtct taatcagcgt ctcagacaaa
    12301 gcgggcattg ttgaatttgc ccaagaactc aaaaaacttg gttgggagat tatctcaaca
    12361 ggtggaacta aggttgccct tgataatgct ggggtggata ccattgctat cgatgatgtg
    12421 actggtttcc cagagatgat ggatggccgt gttaagactc ttcatccaaa tatccacggg
    12481 ggtcttctcg ctcgtcgtga tttggatagc cacttggaag cagccaatga gaaccaaatt
    12541 gagcttattg accttgtagt ggtcaacctt tacccattta aggaaactat ccttaaacca
    12601 gatgtgactt atgctgatgc agttgaaaat atcgatattg gtgggccgtc tatgcttcgt
    12661 tcagcagcga aaaatcatgc cagtgttaca gttgtggtag atcctgctga ctacgctgtg
    12721 gttttggacg aattggcagc aaacggcgaa acctcttatg aaactcgcca acgtttagca
    12781 gccaaagtat ttcgtcacac agcagcttat gacgccttga ttgcagaata cttcacagct
    12841 caagtgggtg aaagcaagcc tgaaaaactc actttgactt atgacctcaa gcaaccaatg
    12901 cgttacggtg agaatcctca acaagacgcg gacttttacc agaaagcttt gcctacagat
    12961 tactctattg cttcagccaa acagctcaac gggaaagaat tgtcatttaa taatatccgt
    13021 gatgcagatg ctgctatccg tatcatccgt gacttcaaag atagtccaac cgttgtggct
    13081 ctcaaacaca tgaatccatg tggaattggt caagctgatg acatcgagac tgcttgggac
    13141 tacgcttatg agtctgaccc agtatctatc tttggtggga ttgtcgtcct caaccgtgag
    13201 gtggatgctg cgacagctga gaagatgcac ggcgttttcc tcgaaatcat cattgcacca
    13261 agctatacgg atgaagcgct agctattttg atcaataaaa agaaaaactt gcgtatcctt
    13321 gccttgccat ttaatgctca agaggctagc gaagtggaag cagaatacac aggtgtagtc
    13381 ggtggacttc tcgtgcaaaa tcaagacgtg gtcaaggaaa gcccagctga ctggcaagtg
    13441 gtgaccaaac gtcagccaac tgagacagaa gcgactgctc ttgagttcgc ttggaaggct
    13501 atcaagtacg tcaaatcaaa tggtattatc gtgaccaacg accacatgac acttggtgtt
    13561 ggtccaggtc aaaccaaccg tgtggcttct gttcgccttg ccattgacca agccaatgat
    13621 cgtctggacg gggcggtcct tgcttcagat gccttcttcc catttgcgga taacgtggaa
    13681 gaaatcgcca aagcaggaat taaggccatc atccagcccg gtggctctgt ccgtgaccaa
    13741 gaatccatcg aagcagcgaa taaatacggc ttgactatgg tctttacagg tgtgagacat
    13801 tttagacatt aagaagataa aagggaagaa aacagtttct ttcctttttt ggcttaaaat
    13861 gctaactgaa acaagattaa aacgaacttt tttgatataa tgttggtaaa taattcgcaa
    13921 aagaggttga ggaatgaaac tgcttgttgt cggttctggt ggtcgtgagc atgcgattgc
    13981 taaaaagtta cttgaatcaa aagacgtgga aaaagtcttt gtagctcctg ggaatgatgg
    14041 gatgactctg gatggtttgg aattggtaaa tatctctatt tccgaacatt ctaaattgat
    14101 tgacttcgca aagaccaatg atgttgcttg gacctttatc ggtccagatg atgcccttgc
    14161 tgctggtatc gtggatgatt ttaaccaagc tgggcttaag gcctttggtc cgactagggc
    14221 tgcagcggag ctggagtggt ccaaggattt tgccaaggaa atcatggtca aatatggtgt
    14281 ttcgacagca gcctatggca cattttcaga tttcgaggaa gcaaaggcct atatcgaaaa
    14341 gcatggtgcg cctatcgtag tcaaggcgga tggcttggcg cttgggaagg gtgtcgtcgt
    14401 tgctgagacg gttgagcaag cggtcgaagc cgctcatgag atgcttttgg acaataaatt
    14461 tggtgactca ggtgcgcgcg tggttattga ggaattcctt gaaggagagg aattttcact
    14521 ctttgccttt gtcaatggtg ataagttcta catcatgcca acggctcagg accacaaacg
    14581 tgcctacgat ggcgacaaag ggcctaacac gggtggtatg ggtgcctatg cgccagttcc
    14641 acacttacca cagagtgtag ttgatacagc ggttgacacc attgtcaagc cagttctaga
    14701 agggatgatt aaagaaggtc gcccttatct gggagttctt tacgcagggc ttatcctgac
    14761 agctgatgga ccgaaagtca ttgagttcaa cgctcggttc ggagatccag aaactcagat
    14821 tatattgcct cgtttgacct ctgactttgc tcaaaatatc acagatatcc tggatagcaa
    14881 ggagccaaat atcatgtgga cggacaaggg tgtgactctg ggtgtggttg tcgcatccaa
    14941 gggctacccg ctagactatg aaaggggcgt tgagttgcca gccaagacag aaggcgatgt
    15001 cattacctac tatgcagggg ctaagtttgc ggaaaatagc agagcactgc tctcaaacgg
    15061 cggacgagtt tatatgctgg ttaccacagt agccaccgtc aaagaagccc aagccagcat
    15121 ctaccaagaa ctatcccaac aaaaaataga aggactcttc taccgaacag atatcggaag
    15181 caaggcaatt aagtaaagat ataagaataa cgcgccgtag tcaccaaaca cgataatggt
    15241 cgtcgtggtg aaaagaccag aacagtgaat gttctggtca gggggaaact tggagacctt
    15301 aggctcaaag tttaggaatg aaaccgaagg tttgcttccg cctccatcac ctaagaccat
    15361 tatcaaaaag aaaaataaaa attcacaaaa tacgttaatg atcgtatggt ttgcgagcgt
    15421 tagcgagcta atatagaaca atcaccgccg ttgtgaaaga acgattggat gataatccaa
    15481 tcgttcaggg aaattggaag accttgggtt tccaatttag gcatgagaca cctttggtgg
    15541 ctgctgccgt ccctcacaag ctaaggtgat tgttgaaaaa gaggaaaaag gagaagaaat
    15601 gaaaccagta atttccatca tcatgggctc aaaatccgac tgggcaacca tgcaaaaaac
    15661 agcagaagtc ctagaccgct tcggtgtagc ctacgaaaag aaagttgttt ccgcacaccg
    15721 tacaccagac ctcatgttca aacatgcaga agaagcccgt agtcgtggca tcaagatcat
    15781 catcgcaggt gctggtggcg cagcgcattt gccaggcatg gtagctgcca aaacaactct
    15841 tccagtcatt ggtgtgccag tcaagtctcg tgctcttagt ggagtggatt cactctattc
    15901 tatcgttcag atgccgggtg gggtgcctgt tgcgaccatg gctatcggtg aagctggagc
    15961 gactaacgca gctctctttg ccctccgtct cctctctgta gaagataagt ccattgcgga
    16021 tgcacttgcc aactttgctg aagaacaagg aaaaatcgca gaggagtcgt caaatgagct
    16081 catctaaaac aatcggaatt atcggtggcg gtcaactggg tcagatgatg gccatttctg
    16141 ctatctacat gggccacaag gttatcgcgc tggatcctgc ggcggattgc ccggcctctc
    16201 gtgtggcgga aatcattgtg gcaccttata acgatgtgga cgccctccgt cagttggcag
    16261 accgttgcga tgtcctcact tacgagtttg aaaatgtcga cgctgacggt ttggatgccg
    16321 ttatcaagga tggacaactc cctcaaggga cagatctgct ccgcatttcg caaaatcgta
    16381 tttttgaaaa ggactttttg tcaaacaagg ccaaagtcac cgttgcacct tacaaggttg
    16441 tgacctcaag ccaagatttg gcagatatcg acttgtcgaa aaactatgtc ctcaagactg
    16501 cgactggtgg ctacgatggt catggacaaa aggttattcg ttcagaagca gacttggaag
    16561 cagcctatgc gctagcagac tcagcagact gcgtcttgga agaatttgtc aactttgacc
    16621 ttgagatttc tgtcatcgtg tcaggaaatg gcaaggaggt gacgtttttc ccagttcagg
    16681 aaaatatcca ccgcaacaat atcctgtcta agaccatcgt acaagcccgc atttctgaaa
    16741 gtctagcaga caaggctaaa gctatggcag tgcgaatcgc agaacaactc aacttgtctg
    16801 gaactctctg tgtggaaatg tttgcgactg atgatgacgt tattgtcaat gaaatcgccc
    16861 cacgaccaca taactctggc cactactcga tcgaagcttg cgacttctcg cagtttgata
    16921 cccatattct gggtgttctg ggagcgcagt tgccagctat caaactccat gcgccagctg
    16981 tcatgctcaa tgtcctaggt caacacgtcg aggctgctga aaagtatgtt gcagaaaatc
    17041 caagcgccca cctccacatg tatggtaaaa tagaagcaaa gcataatcgt aagatgggac
    17101 atgtgacctt gtttagtgat gtaccggata gtgtggatga gcttggggaa gggattgatt
    17161 tttaggacaa gtctatgata caaattatcg ttaatacatt tattgaaaag gataagactg
    17221 gagcagttgt tgaagtgttg tatgccagtg ctgaccaaga taaggtacaa gctaaatatg
    17281 aagaactagc tgcacaatac cccgaaaatt atttagctat ctatgatgta ccgctggata
    17341 cggatttgaa tacactagat cattacccgt ctgtgtttat tggaaaagag gagtttgagt
    17401 agaaatcttg gtttacctag atagcttatt cccaacagct taagaagaaa ggaaaaatta
    17461 acacatgatc aaccgttact ctcgccctga gatggcgaat atttggagtg aagaaaataa
    17521 ataccgtgct tggcttgagg tggaaatcct ctctgacgag gcatgggctg agttggggga
    17581 aatccctaag gaagatgtgg ctttgattcg caagaaggcg gactttgaca tcgaccgtat
    17641 tttggaaatt gagcaggaga cgcgccacga tgtggtggct ttcacgcgtg cggtttctga
    17701 gactcttggt gaagagcgca agtgggttca ctatgggtta acttctaccg acgtggtgga
    17761 tactgcttat ggttacctct acaagcaggc caacgacatc atccgtcgtg accttgaaaa
    17821 cttcactaat atcatcgctg acaaggccaa ggagcacaag ttcaccatca tgatggggcg
    17881 tactcatggt gtgcacgctg agccgacaac ctttggtctt aaattggcaa cttggtacag
    17941 cgaaatgaaa cgcaatatcg agcgcttcga gcatgcggct gctggtgtag aagctggtaa
    18001 gatttctggt gcggttggga actttgccaa tatcccacca tttgtagagg agtatgtctg
    18061 cgataaactt ggcatccgtg cccaagaaat ctctacacaa gtccttcctc gtgaccttca
    18121 cgctgagtac tttgcggttc ttgccagcat tgcgacttca atcgaacgta tggcgactga
    18181 gattcgtggt ctacaaaaat ctgagcaacg cgaagtagaa gagttctttg ctaaagggca
    18241 aaaagggtct tcagcaatgc ctcacaaacg caacccaatc ggttctgaaa atatgactgg
    18301 tctggcgcgt gtcattcgtg gtcacatgat tacggcttat gaaaacgtcg ctctctggca
    18361 cgaacgcgat atttctcact catcagctga gcgtatcatc acaccagata cgaccatttt
    18421 gattgactac atgctcaacc gttttggaaa tatcgtcaag aacttgacag tcttcccaga
    18481 aaatatgatc cgaaacatga actcgacttt tggtcttatc tttagccaac gggctatgtt
    18541 gacattgatt gaaaaaggca tgacccgtga gcaagcctat gacttggtgc aaccaaaaac
    18601 agcctactct tgggacaacc aagtagactt taaaccactt cttgaggcag attcagaagt
    18661 aacatcacgt ctcacacaag aagaaatcga tgaaatcttc aacccagttt attacaccaa
    18721 acgagtggat gatatctttg aacgtcttgg actaggtgat taattaaaaa ataaacagcg
    18781 agcttcaatc tcgctgttta ttttttatcg aaaagactta gtcttctttt cttttagtga
    18841 gtccataggc tgctagtgtg gccatgagtc ctgcgactac tagtcctgca gaatcgtgag
    18901 ttcctgtttc aggaagtttt ttctctatta ccacaggagc tggatcttga ggaagagctt
    18961 tgctttcctc agcaggagct ggttggctta ggatttctag ttttggtttt tcttcagcaa
    19021 tagcggcttg tccgttttca tcgcctacat gtgttaccat agttccgact tcgactattt
    19081 gagtaacggc ttcctgtgct acgacactat ttacaagtgt tttcacttcc ttaccatcgg
    19141 cagaagtgct cacagagtag aagttgctac gatgtccatt gacgccctta gtaatgactt
    19201 gtgtttttcc tttgagtaag agtggatttt cacgagtcac tgtggtaaat ggaatttctt
    19261 cttcttggat atccagtcta ggttttacct cagtagttgg tgcaagacca ctttcatcac
    19321 ccttgtgagt tacaggagcg ccaacttcaa ccacttggtt tataacttct ttggttacct
    19381 ggctatcaag gactgtttct gttgtttttc cattttcagt gagtacagag atgtaatgag
    19441 ttcgttcacc tttgactcct gctgtgataa tattttcctg accagctggg aggttaggat
    19501 tttctttctt gataacttca aatggaattt cttcagttct tgtgatgagt tctggtctgg
    19561 tttcaacatt ggcagccact tcattttcat ctaggcttcc tgaatgagtt gcagctggtt
    19621 tgaggccttg aagagcggct tttaggttgg ctacaagtgt gtcaagctca gcttgtttat
    19681 tacggttgag gttgtaattt agagctgttt tagctgcgtc aagggcctca agactttctt
    19741 tactatatcc ttctaagttt gtaggaattt tagctaattc ttcgcggaga gcattataat
    19801 tagcacggaa gtagtctttg ttgtggtctg caaaggcagt catgagttca aagatttcct
    19861 cttccttgta ttcagcgctt ggtctatctg cccagattga aagcatactt ccgactgttg
    19921 gaagatctac ttcaggatat ttggtagaag ctagttgatt gaatggaact ttaccagaat
    19981 tttcaactgc ttttgaaaga gggtaagctt catcttgttt gtgattgcct ataacataat
    20041 accagtcacc gttggtattc aagaacttat aacccttgct tgctaggtat tgaggtgatg
    20101 cgaggttgta tccccaccag cctttagacc agtaagaaat caagacatct ttgtcaaact
    20161 gaacatcgtc cttgtcttca tagtagaagc catcgttgaa ggccattggt tgaagccctc
    20221 tttctttggc catagctgcg agggtgttgg catattcggc aaatttgcca tagagttgat
    20281 accacttgag gtagtaccag ccttgggcac tagtcgcatc gttggcgtat tcgtcagtac
    20341 caaagttgaa aatctttgtt ttacctgcaa agaagtccat gtatttaccg atgagggctt
    20401 ttacaaagtt catcgcttct tcgtttttca agtccatagt tgtttttgaa actttatcaa
    20461 agtgggcttg aggattttta atacctaatt tttccatggc aaccagcata gcatccatgt
    20521 gacctggact gttaatagct gggatgagac caatgtcctt agatttagcg tattcaatta
    20581 gctctgtcac ttctgcctgt gttagtgcag taccgtttgg atcgtcgtag taagctttag
    20641 ttccttcgat aatagctttt ttaacgtcat cactagcata ggtttttccg ttggcagtaa
    20701 tggtcatatc attgagtaga aagcgaagtc cgtcatttcc tagaaggaga tggacatcag
    20761 aatatccgag ctcactggcc ttgtctacga tgcgtttgag ctggtccaga gtaaagtatt
    20821 tgcgtccagc atcgattgag attaccttgt ttttggcaag tttttcaacc tcacgtttag
    20881 cttcttcttc tttttgagct tcaggcgtga gggtcaagtt gttgacagtt tcttgaagtt
    20941 tagcaatggc tttatcaatc gtatcttgtt gggcacggct aaggttgcta tcgagagagc
    21001 gaatagcttt ttcagcttct tttacggccg tgacgctttc tgcagtataa cggttcaggt
    21061 cttttggtac ctcgttaagt gcttgctctg cagattcata atcagctgcg aagtattcag
    21121 cgttggcatt tgcaaaatga cgcatgagtt tgaagaggcg tgatggtgaa taacgtgcag
    21181 atggagtgtc agcccaagca gctaccatac caccgatgat tgggatatca gctccttctg
    21241 tttttggtac agaagtgatt ggtgtgtttt taataccatt gagcccctga tcgagattgt
    21301 accagccttg gccatcagcg tttcgtccaa gaacgtagta ccaagcatca ttggtattaa
    21361 ggatttggtg acctttttca gctagtagtt tagaagaagc gacatcgtag cctccccaac
    21421 caccagtcca catagaaacg atgatgtctt tgtcaaaact accaaagctt gtgtcgctat
    21481 tgtagtagat accgtcgtta aaggccattg gtttgagacc gtgcgatttt acaatacgag
    21541 cgaggtcatt ggcgtaggca ataaattttt catagccttt tacagggtag ccttcgtttg
    21601 gatagtattt atcagcttga agcacactcc aacctttagc atctgtcgca tcattggcat
    21661 attcatcaag tccgatgttg aagatttcag tctttttcgc gaaataagca gcatacttgt
    21721 cgataagggc ttttgtaaaa gcgacagctt gttcgttgtc aagatcgaca gtacgggctg
    21781 atttcttccc aaaatagcta aagttagggt tttggattcc caattctttc atggcattga
    21841 gaatcgcatc catgtgtcca ggactattta ctgtcggaat gagaccgata cctttatctt
    21901 tggcatagtt aatcagatct gtcatttgac tttctgttaa gtgattgccg tttggatcgt
    21961 tgtaataatc atttgtacct ttttcaatgg cgcgtttgac atcgtcactg gcataggtct
    22021 tgccgttagc tgtgatgctc atatcgtcca acatgaaacg gagtccatca tttccgacta
    22081 ataggtgtaa atcagtgtag ccataatgtt tcgctttatc gatgatttcc ttgagctgtt
    22141 ctggtgagaa atatttacgt ccagcatcaa tagaaacaat tttctttttc gctagttttt
    22201 catttacagt tgcagcacgt tcctttcctg tctctgtttc cggtttgtca gcctctgctt
    22261 tcgcttcatc ttttttagct ggtttatcct tgtcagtctt gtctgtattt gactctttag
    22321 aatcaacctc tttcgcttct tcctttttag ggctagcttc ttcttccttt ttattagcag
    22381 tttctttttc agcagaagtt ggagttacca cctctgcttt atcactagga gttgaactaa
    22441 cttcctcttg tggtttttct tctgtttttg gaagactagc taccttatca gtagctggag
    22501 tttctgtttc tacagttttt ggagcttctg gttgaagctc tgctttaggt gtttcctcag
    22561 tccgattttc ggatgattga ggggaatcag aaaccgtatg gatggtcggt tggttttctg
    22621 tagtagtagg agtaactcca tcggctgcaa cagtctgtgc ttggaaggca aatccaatta
    22681 gaacagaagc tgctcctaca gcgtatttac gaatagaaaa acgctgttgt ttttcatgtt
    22741 tcattgcaaa acctcctgat tgcattgtta tattgatagc gattatataa atcaacgcct
    22801 ttattttatt tcttatatta acgagagtca agaggagatg acaaaaaact ataataagta
    22861 t
//
LOCUS       DAVMEI010000016        21178 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVMEI010000016 DAVMEI010000000
VERSION     DAVMEI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.016"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            49..537
                     /locus_tag="VYQ24_000511"
     CDS             49..537
                     /locus_tag="VYQ24_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1967691.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            534..1721
                     /locus_tag="VYQ24_000512"
     CDS             534..1721
                     /locus_tag="VYQ24_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1967692.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     CTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            1714..1938
                     /locus_tag="VYQ24_000513"
     CDS             1714..1938
                     /locus_tag="VYQ24_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1967693.1"
                     /translation="MIDHFEIKVKDLQISGGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            2095..3441
                     /gene="asnS"
                     /locus_tag="VYQ24_000514"
     CDS             2095..3441
                     /gene="asnS"
                     /locus_tag="VYQ24_000514"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1967694.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            3594..3884
                     /locus_tag="VYQ24_000515"
     CDS             3594..3884
                     /locus_tag="VYQ24_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1967695.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            3896..4366
                     /gene="ssbA"
                     /locus_tag="VYQ24_000516"
     CDS             3896..4366
                     /gene="ssbA"
                     /locus_tag="VYQ24_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1967696.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            4398..4637
                     /locus_tag="VYQ24_000517"
     CDS             4398..4637
                     /locus_tag="VYQ24_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1967697.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            4932..6332
                     /locus_tag="VYQ24_000518"
     CDS             4932..6332
                     /locus_tag="VYQ24_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1967698.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            6334..6693
                     /locus_tag="VYQ24_000519"
     CDS             6334..6693
                     /locus_tag="VYQ24_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1967699.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            6747..7496
                     /locus_tag="VYQ24_000520"
     CDS             6747..7496
                     /locus_tag="VYQ24_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1967700.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            7486..7785
                     /locus_tag="VYQ24_000521"
     CDS             7486..7785
                     /locus_tag="VYQ24_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1967701.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            7856..8791
                     /locus_tag="VYQ24_000522"
     CDS             7856..8791
                     /locus_tag="VYQ24_000522"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1967702.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            8877..9389
                     /locus_tag="VYQ24_000523"
     CDS             8877..9389
                     /locus_tag="VYQ24_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1967703.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            9426..10022
                     /locus_tag="VYQ24_000524"
                     /pseudo
     CDS             9426..10022
                     /locus_tag="VYQ24_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            10123..10317
                     /locus_tag="VYQ24_000525"
     CDS             10123..10317
                     /locus_tag="VYQ24_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1967704.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVSKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(10363..11808)
                     /locus_tag="VYQ24_000526"
     CDS             complement(10363..11808)
                     /locus_tag="VYQ24_000526"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1967705.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYIAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISLDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FRFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            11914..13536
                     /locus_tag="VYQ24_000527"
     CDS             11914..13536
                     /locus_tag="VYQ24_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1967706.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            13739..15697
                     /locus_tag="VYQ24_000528"
     CDS             13739..15697
                     /locus_tag="VYQ24_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967707.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKSKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQACLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            15869..16825
                     /locus_tag="VYQ24_000529"
     CDS             15869..16825
                     /locus_tag="VYQ24_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967708.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDMSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFEGGKSVSG"
     gene            16829..17551
                     /locus_tag="VYQ24_000530"
     CDS             16829..17551
                     /locus_tag="VYQ24_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1967709.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGKKIAFVGPSGSGKSTIV
                     RLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            17708..18802
                     /locus_tag="VYQ24_000531"
     CDS             17708..18802
                     /locus_tag="VYQ24_000531"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1967710.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            18810..19976
                     /locus_tag="VYQ24_000532"
     CDS             18810..19976
                     /locus_tag="VYQ24_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1967711.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSIKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            20241..>21178
                     /locus_tag="VYQ24_000533"
     CDS             20241..>21178
                     /locus_tag="VYQ24_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1967712.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HTEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSE"
BASE COUNT         6328 a         3899 c         4725 g         6226 t
ORIGIN      
        1 ttcttatatt cacttgtaat aaagcttaat agatggtaaa atagacgagt gaaaaaaaga
       61 caaaacaaag caaaaaataa tctactgtgg cagtatggtc tagggatgac gattttgttt
      121 gtggttatca gtgcttcctt tctgtatatg gtttctctta gcatgaaacc ctatcaaaca
      181 gctaaaagtg aaggagaaaa attagctcag cagtatgcag gattagagca ggctgatcag
      241 gttgatttat acaatggctt ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa
      301 gaagcgcttg ctgtcctgat tggtaaagat gaccataaga tttacgttta tcagctaaat
      361 cagggtattt cacaagaaaa agcagaaacg gtttctaagg aaaagggagc tggcgagatt
      421 gacaagataa cctttggtcg ttatcaagac aagccaatct gggaagttaa gtcaggatct
      481 gatttttatc tagtagattt tgaaacagga gcattggtca acaaggaggg cctatgaaat
      541 tatccaaccg tgttttagaa atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca
      601 aagcactgaa ggctgagggt agagatattt tgtctctaac cttgggtgag ccagatttca
      661 ctactcccaa aaatatccaa gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt
      721 tttatacagt aacctcaggt ctgccagagc ttaaggcggt ggtcaatagc tactttgagc
      781 gcttttacgg ctattctgta gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt
      841 ctctctatac cttctttatg gctgtggtca atccaggtga tgaagtcatc atcccaaccc
      901 catactgggt tagctatgga gatcaggtca agatggcaga aggcgttccc gtctttgttt
      961 ctgctaagga agacaatcac tttaaggtga ccgtagagca gttagaagca gcttgcactg
     1021 acaagaccaa ggttttggtg ctgaattcgc catctaatcc cacaggtatg atttacaccc
     1081 gtgaggaact cttggcaatt ggaaactggg ctgtagaaaa tgatattctc atcctagcag
     1141 acgatatcta tggccgcttg gtttataatg gtcatgagtt cacaccgatt tctagcctat
     1201 cggaagcgat tcgcaagcaa acagtggtca tcaatggtgt gtctaaaacc tatgccatga
     1261 ctggttggcg gattggttat gccgttgggg aagcagacat tatcgctgcc atgtccaaga
     1321 ttgcaggtca aacaacttcg aatccgtcag cagtagccca atatgcagca gttgaggctc
     1381 tatcaggtga gcaagatact gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata
     1441 ccatctatcc cctccttgca gaagtgccag gatttgaagt ggtcaaaccg caaggggcct
     1501 tctatctctt cccaaatgtc aaaaaggcca tggagatgaa aggctacacg gatgtgacag
     1561 actttacaac tgttatctta gaagaagccg aagtggcctt ggtaacagga gctggatttg
     1621 gagcaccaga aaatgtccgc ctcagctatg cgacagacct agacacgctt aaagaagcag
     1681 tcgaacgctt gaaagcattt atgggtagtg agaatgattg atcattttga gattaaggta
     1741 aaggatttac aaatttcagg aggattttat aggagttttc tcgctccttt ggactataaa
     1801 ttggctttta agactagttc tctaattagt tttctatccc cgaacagccc tcatcctggt
     1861 ggtgattttt ggctgactca aggaacacaa gatcctgttc attttgcttt tttagcagaa
     1921 aataaggaag aagtttaggc ttgttatgag gctggcttag aggcaggtgg gcgagacaat
     1981 ggggctcctg gttatcgaag tgagcgtccg atttactatg ctgcttttat gattgacctg
     2041 gatgggaaca atatagaggt ggtttgccat aaagaataaa aaagaaaagg aaaaataatg
     2101 acaaaacgtg taacgattat tgacgtaaaa gactatgttg gtcaggaagt gacgattggc
     2161 gcttgggttg ccaacaaatc aggaaaaggg aaaatcgctt tcttacaatt gcgtgatgga
     2221 acagccttct ttcaaggtgt ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa
     2281 gtgggacttg agaagtttga tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg
     2341 acaggtattg tcaaagagga cgaacgttct aaatttggct atgagttgga catcacagac
     2401 atcgaagtga tcggtgaatc tcaagactac ccaatcacac caaaagaaca cggaacagac
     2461 tttttgatgg ataaccgtca cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa
     2521 atccgtaacg ctattatcta tgcaacttat gagttctttg acaagaacgg ttttatgaag
     2581 tttgacagcc caattctttc aggaaatgcg gcagaagatt ctacagaact ctttgaaact
     2641 gactacttcg gaacgccagc ctacttgagc caatcaggtc agctttacct agaagcaggg
     2701 gctatggctc ttggtcgtgt ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa
     2761 acacgccgtc acttgactga gttctggatg atggatgctg agtactcata cttgacacat
     2821 gatgagtcgc ttgacttgca agaagcttat gtgaaagctc ttctacaagg tgttcttgac
     2881 cgcgcgcctc aagccttgga aaccttggaa cgtgatacag aactcttgaa acgctacatt
     2941 gcagagccat tcaaacgtat cacttacgat caagccattg acctcttgca agagcatgaa
     3001 aatgatgaag atgctgacta cgagcatctt gagcatggtg atgactttgg gtcaccacac
     3061 gaaacttgga tttcaaacca ctttggtgtg ccaacatttg tcatgaacta tccagcagcc
     3121 atcaaggcct tctacatgaa accagttcct ggaaatccag agcgcgtgct ttgtgcagac
     3181 ttgcttgctc cagaaggcta tggagaaatt atcggtgggt ctatgcgtga ggaagattac
     3241 gatgcccttg tcgctaagat ggatgaactt ggcatggatc gtacagaata tgaattctac
     3301 cttgaccttc gtaaatacgg tacagttcca cacggaggat ttggtatcgg tatcgaacgt
     3361 atggtaacct tcgcagcagg aacaaaacat atccgtgaag ctattccatt cccacgtatg
     3421 ttgcaccgta tcaaaccata agaacaggaa aacgcccatg tggcgttttt tcgaattcta
     3481 gtgtttttac ttgcgtaaaa aaattttttc tagtataata gtttattgtg agcgaacctc
     3541 acttacccct tgcaaagtct tggggtcatt agaccaaaag gaggaacata tcaatggcta
     3601 aatacgaaat tctttatatc attcgtccaa acattgaaga agaagctaaa aacgctttgg
     3661 tagcacgttt tgactctatt ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt
     3721 gggaaaaacg tcgtcttgca tacgaaatcc aagatttccg tgaaggactt taccacatcg
     3781 ttaacgttga agcaaatgac gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca
     3841 acgctgacat tcttcgtcac atgatcgtca aaattgacgc gtaagaaggt aaactatgat
     3901 taacaatgtt gtacttgtag ggcgtatgac acgtgacgct gaattgcgtt ataccccatc
     3961 aaatgtagca gttgcgactt ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg
     4021 tgaacgtgag gctgatttta tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc
     4081 taactgggct aaaaaaggct cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta
     4141 cgataaccag caaggacaac gtgtctacgt gacagaggtc gtggctgaga atttccaaat
     4201 gttggaaagc cgtagtgtgc gtgagggtca cacaggtgga gcttactctg caccaactgc
     4261 aaactattca gcacctacaa attcagtacc agacttttca cgtaatgaaa atccatttgg
     4321 agcaacaaac ccattggata tttcagatga tgatttacca ttctaatgga caactaaatt
     4381 tataaaggag aaaaaacatg gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag
     4441 ttgattacat cgcagcaaac aaaattgaat atgttgatta caaagatact gagcttctta
     4501 gccgtttcgt ttcagaacgt gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta
     4561 aaaaccaacg taaagtaaca acagctatca aacgcgctcg cgtaatggct ttgatgcctt
     4621 tcgtaaacga agattaaaga aaagacccag ttgggtctct ttttcatgag cttgaaaaca
     4681 agaaagcgaa ttaagacccc aacgggtctt ttttcacaag ctttgaaatg agagagcgag
     4741 ttaggctcag tggctccctt tttacgagtt tgaaaacaag agaacacatt aaaagcacga
     4801 tggagctttt tcataaatat gtctcttttc tcatccaaga aattaaaata aaaatgataa
     4861 actttttgaa aataggggat ttgcctactt tatgatataa tggaaagagt tagattgaaa
     4921 gaggtagtga gatggacgcg aaattaagat acaaggcaaa gaagatcaag attgtctttt
     4981 ttgatattga tgatacattg cggaattcaa agacagggtt tattccaact acaattccca
     5041 ctgtttttaa acagttgcgt gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca
     5101 tttttggtgt tgttccagag attcgtgatc tcaagcctga cttttttgta actttgaatg
     5161 gggcttatat cgaagataaa aaaggtcagg tcatttatca gcatcagatt gaaaagtcag
     5221 atgttgagga gtatatctct tgggctaagc aagaaggaat tgagtatggc ttggttggga
     5281 gtcatgatgc caagttgtcg actcgcaccg atatgatgag tgaagctatc aatccaattt
     5341 atcccgactt agatgtagat cccgatttcc atgaaaaaga agatatctat cagatgtgga
     5401 cttttgaaga taagggagat gacttgcact tgcctgacag tctctcagac aaacttcgca
     5461 tggttcgttg gcatcaacat tcgtctgata ttgtgccgat ttcaggctcc aaagcgacgg
     5521 gggtggaaaa ggttgtggaa caccttggct tgaaaccaga gaaggtcatg gtttttggag
     5581 atggtctcaa cgacttggaa ctctttgatt atgctggaat cagcgttgcc atgggaattt
     5641 ctcatgataa aatcaaagaa aaagcagatt atattacaaa aacattagaa gaagatggca
     5701 tttttgctgc cttagaagta tttggtatgg tagaaaaaga attgcatttt ccacaagtag
     5761 acattgaaac agtagaaggt cctcttgcga ctattaagac caatcacgga gacttacgta
     5821 tcaagctttt ccctgaacat gctcctaaaa cagtggctaa ctttgtatct ctttcaaaag
     5881 atggctacta tgatggtgtc attttccacc gtattatcaa ggactttatg atccaaggtg
     5941 gagacccaac tggaactggt atgggtggcc agtcaatcta cggcgaatct tttgaggatg
     6001 aattctcaga agagctttac aatatccgtg gtgctctttc catggcaaat gctggtccaa
     6061 ataccaacgg cagccagttc tttatcgtgc aaaaccaaca cctaccttat tctaagaaag
     6121 aaattactcg tggtggttgg ccagaaccga ttgcagaaat ctatgccaat caaggtggga
     6181 cacctcacct agaccgccgt cacacggttt ttggtcagtt agctgatgaa gcatcttacg
     6241 ctgtcttgga tgccattgct gctgttgaga caggagctat ggacaagcca gttgaagatg
     6301 ttgtaattga aactattgaa atcgaggact aagatgaaaa tcggtgataa gctaaagggg
     6361 cgtattacag ggattcagcc ctacggtgcc tttgttgagt tagagacggg tgatacgggg
     6421 ctgattcaca tttcagaaat tcggacagga tttattgaaa atattcatga aaccttgaaa
     6481 gtcgatgaag aggttcaagt tcaggtagtg gatttagatg aatttacggg gaaagccagt
     6541 ctttctatcc gcactttgga ggaagaaaag taccagtttc ctagacggag acgcttttca
     6601 agcgaccgct ttaactacgg ttttgctccc tttcgacgaa tgctaccaat ctggactgga
     6661 gaagccctgc accatttaaa aaagaagaag tagtttagag ttttatattt tttgtaatgt
     6721 taatatgaat ttgctataat aggaccatgg aagaagaaca attattaaaa tcaggagagc
     6781 gcattaacca gctcttttcg acagatatca aaatcattca aaatagagag gtttttagct
     6841 attcggtgga tagtgttctc ttatcacgat ttccacgttt tcctaagaag gggttgattg
     6901 tggatttctg tgctgggaat ggagcagtgg ggctttttgc tagcactcgt actcaagcac
     6961 agatattgtc tgttgagatt caggagcgtt tggcggatat ggctgaacgc tctgtccgtt
     7021 tgaatggatt agaggagcaa atgcaggtca tctgcgatga tttgaaaaat atgcctgctc
     7081 acattcaggg gagtaaggtg gatatgattt tgtgtaatcc accctatttc aaggtggatc
     7141 cttattctaa tctgaacgag agtgaacatt atctcttggc tcgacacgaa atcacgacta
     7201 atttggaaga aatctgtcgt agtgcccaga gtattctcaa gtctaatggg cgtttggcca
     7261 tggttcatcg tcctgatcga cttctggata ttttggatac gttaaaacga cataatctag
     7321 cccctaagcg cctgcagttt gtttatccaa aaagagaaaa ggaagccaat atgcttttga
     7381 ttgaggcgat caaggatggc tcaacaagtg gctttaaggt cttacctcct ttgattgtcc
     7441 acaatgatga tggctcttat acgcccgaaa tcgaagagat ttattatgga tcataaggct
     7501 tatatgtacg ttctggagtg tcgtgatgga tcctactata taggctatac gactgatatg
     7561 agaagacgcc tcgctatcca caatagtggg aagggagcca aatatacacg agcacgcttg
     7621 ccagtcaaac ttatctatgc tcaaggtttt gccagtaagg aagaagccat gtcggctgaa
     7681 gctcttctca agcgtaagaa gaggccacag aaggaagaat ttttatctga aaatcaagat
     7741 agaaatttac tcagttattt tgaagaatcg tggggagtcc tttgactcct cttacctttg
     7801 tcaaaaagcg aaaaaaatgc tacaataaag aaaataaaca aaatgaggta ttatcatgtc
     7861 caagattcta gtatttggtc accaaaatcc agactcagat gccatcggat catctgtagc
     7921 ttttgcctac cttgcaaaag aagcttacgg tttggatacg gaagctgttg cccttggaac
     7981 tccaaatgaa gaaacagcct ttgtcttgaa ctattttggt gtggaagcac cacgtgttat
     8041 cacttctgcc aaagcagagg gggcagagca agttatcttg actgaccaca atgaattcca
     8101 acaatctgta tcagatatcg ctgaagtaga agtttacggt gttgtagacc accaccgtgt
     8161 ggctaacttt gaaactgcaa gcccacttta catgcgtttg gagccagttg gatcagcgtc
     8221 ttcaatcgtt taccgtatgt tcaaagaaca tggtgtagct gtgcctaaag agattgcagg
     8281 tttgatgctt tcaggtttga tttcagatac ccttcttttg aaatcaccaa caacacaccc
     8341 aacagataaa atcattgctc ctgaattggc tgaattggct ggtgtgaact tggaagaata
     8401 tggtttggca atgttgaaag ctggtaccaa cttggctagc aaatctgctg aagaattgat
     8461 tgatatcgat gctaagactt ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa
     8521 cacagttgac atcgctgaag ttttggaacg ccaagcagaa attgaagctg caatgcaagc
     8581 tgccaacgaa tcaaacggct actctgactt tgtcttgatg attacagata tcgtcaactc
     8641 aaactcagaa atcttggctc ttggtgccaa tatggacaag gtcgaagcgg ctttcaactt
     8701 caaacttgaa aacaatcatg ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt
     8761 acctcaattg actgaaagct ttaatgcgta agattttggg tgtcagctca aaatcggaaa
     8821 gtctagtttg ccttatatcg aaaggagttt cggctccttt tttctaggag tgaagtatgt
     8881 tagaaaatgg cgatttgatt tttgtgagag atgggtcaga catgggacag gccatccaga
     8941 cttccacagg taactatagc catgttgcca tttatttgga tgggatgatt tatcatgcta
     9001 gtggacaggc tggtgttgtc tgtcaagaac cggcagactt ctttgagtcc aatcatttat
     9061 acgacctcta tgtttaccca gaaatggata tccagtcggt gaaggaaaga gcttgcaaac
     9121 atcttggagc accctacaat gcttctttct atccagatgc agctggtttt tactgctccc
     9181 agtatatagc agaaatccta cctatttttg aaactattcc tatgaaattt ggagatgggg
     9241 agcaggagat tagtgatttt tggagggagt attacataga actaggtctg cctgttcctc
     9301 tgaaccaagc tggtaccaat cctagtcagt tggcagcatc gcctctgtta caatgtaaag
     9361 aaaggaatct tcatgattca gatttttaat tcatctcgtt tgacgagaca gccatttttg
     9421 gagaattgat ccgctatctg gatcagtatg aggatgtgat tctacgggaa attaaggctc
     9481 aatttccaga tgttgcagtt gataaactca tggaagagta tataaaggca ggcttgattc
     9541 tacgtgaaaa taagcgctat tacctcaatt ttcctacgct tgaatcactt gatagtcttg
     9601 aactggatca agagattttt gtcagagaag ctagtccggt ctatcaagcc ttgttggagc
     9661 agagttttga gacggaattg cgcaatcaaa tcaatgcagc tattttagtt gaaaagacgg
     9721 actttgcgcg cattaaaatg accctgtcca attattttta caaggtcaaa cagcagtatc
     9781 ctttgacaga aaaacagcag gagctctatg acattttagg agatgttaat cctgagtatg
     9841 ccctcaagta tatgacggct tttttgttga aatttctcaa aaaagaccag cttatgcaga
     9901 aatgccgtga tatctttgtg gacagttagg ttgtcttagg ctatattgtg caaaatgaag
     9961 atggaaagta tgagttggct atcgattttg ataaggagag gttaactttc tacttagcgt
    10021 gatttcttgt ttctgagtac attgtttgac tttccttagt attcggtata aactatatgt
    10081 aaccggtaac acatatcgga ataaactaaa ggagacaatc atatgtcact tgaaaacaaa
    10141 ttggaacaag caacaggcgc tgtcaaagaa ggttttggta aagttactgg agacagcaag
    10201 acagaacttg aaggagctgt tgaaaaaaca gtttctaagg caaaagacgt tgtagaagac
    10261 gcaaaaggtg ctgtagaagg tgccgttgaa ggtttgaaaa acgtttttac taaataatag
    10321 gaaaaaatca agggtttcat tttcccttga ttttttctat tcttataaat aattttctgc
    10381 gacggctgta tctcctgggt aggattcttt cttgccctgg atgatttggt aacaatcggc
    10441 tcccttaccc gcaataataa ctgcatctaa ttcgtgattt gtgatagcca ttgccgcctt
    10501 gatggcttct tggcgatccg caatcttctc aacaggatga ttgatgtagc tactaatttc
    10561 atctgcaatg gccattgggt cttcatagtt agggtcatca gcagtcagaa agacttgaat
    10621 ctcagggtgt tgattgagga ggaggccaaa gtccttacga cgactttctc ccttgtttcc
    10681 tgttgatccc agaaccagag caatctttcc ggtttgatga gtttcaacca cattgatgag
    10741 ttttttcaga ctatccccat tgtgggcata gtcgatgaag accttggctc catttttctg
    10801 agtgaggact tccatacgac caggaacgcg ggttgcagcg atgccttttt tgatgtcctc
    10861 aagacttgct ccgagacgga gacaagcaag tccagcagca actgcatttt cttggttgaa
    10921 gttgccaatg agttggatat cataatctcc agcgagttta cccgtagctg aaaatctaaa
    10981 ggctttggaa ttctcgattt ggttatcaaa ttggctacca tagaaatcat ggtcttgatc
    11041 ttcaacctgt tctttcaaga ctgagaagtg gtccatgtca ctgttaatga tgactgctcg
    11101 gctattttcc atcaagagac gcttgtggta gaaatagtct tcaaagctag ggtgttcaat
    11161 cgggccgata tggtctaggc tgatatttag gaaaactccc acatcaaagg ttagaccata
    11221 gacacgtttg accagatagg cttgactgga gacttccatg atgaggtggg tacggtcatt
    11281 ttgcacagcc tgattcatca tgtcaaagag gtcaatactc tcaggggttg tcaacgctga
    11341 cttaaagaaa gtctctccat caagagttgt gttcatggtc gacaacatag caggtcgatg
    11401 cccttgagat aagatgttat aggcgaaata ggctgctgtt gtcttaccct tagtaccagt
    11461 aaaggcaagg agtttgagtt tttcctgtgg attaccatag aactccatgg caatcaaact
    11521 catggctttc tttatatcgt tcacaatgat gacagggata ccgacttcgt agtccttttc
    11581 agctatatac caagctaagc cttgtgttat agcagaaaga aggtattctt ttttaaaggc
    11641 agcgcctttt gcaaaaaaaa gagtgtcttc tgttactttt cggctgtcgt agctgatgct
    11701 atcaaaaaca actttgctgt agttgtagtg gtaatgacct tggtcaataa tttcgcgaaa
    11761 aaggccatct ttctttaaaa tatctaatac agtttcaatt tttatcatac tttctattgt
    11821 aaaccgaaag tcgcaaattt acaagtgaca aggaaaagtt tataatggaa gataaggagt
    11881 ttttcctagt tatcaaaatt gaatgaggaa tctatgtcgc acgaaaacaa tcaccagcag
    11941 gcccagatgt tacgggggac tgcttggcta acggctagta actttatcag tcgcctactc
    12001 ggggctgttt acattatccc ttggtacatc tggatggggg cttatgcagc taaggcaaat
    12061 ggtctcttta ccatgggtta caatatctat gcttggttct tgttggtttc aacagcgggg
    12121 attccagttg cggtggccaa gcaagttgcc aagtataata ccatgcgaga agaagagcat
    12181 agctttgccc tgattcggag cttcttaggc tttatgacag gactaggcct ggtttttgct
    12241 ttagtcttgt atgtctttgc tccttggcta gcagacttgt ctggcgtggg caaagacttg
    12301 atcccaatca tgcaaagctt ggcttgggga gtcttgattt tcccgtctat gagtgttatc
    12361 cgaggatttt tccaagggat gaataacctc aaaccctatg ccatgagcca aattgctgag
    12421 caggtcattc gtgttatctg gatgctccta gcaaccttta tcattatgaa gctcggttca
    12481 ggagattatc tagcagccgt tacccaatca acctttgctg cctttgtcgg tatggtagcc
    12541 agttttgcag tcttgattta tttccttgcc caagaaggtt cactcaaaag agtctttgaa
    12601 acaggagata agattaacag taagcgtctc ttggttgata ccattaagga agccattcct
    12661 tttatcctga cagggtctgc catccagctc ttccagattt tggatcagct gacctttatc
    12721 aatagtatga gctggtttac caactacagc aatgaggact tggttgtcat gttttcttat
    12781 ttctcagcca atcctaataa aatcacgatg attttgattt ctgtaggggt ttcgattggg
    12841 agtgttggtt tgccactttt gacggaaaac tatgtcaagg gggacttgaa agcagcttct
    12901 cgtctcgttc aggacagtct caccctactc tttatgttct tgctaccagc aacagttgga
    12961 gtggttatgg taggagaacc tctttatacg gtcttctatg gtaagccaga tagtttggct
    13021 ctgggcttat ttgtctttgc agttttgcag tctattattt taggcttgta catggtcttg
    13081 tctccaatgc ttcaggccat gttccgcaac cgcaaggccg ttctctattt tatctatggt
    13141 tctattgcca agctagtctt gcaactacct accatcgccc tcttccacag ttatggtcct
    13201 ttgatttcaa caaccattgc tctcatcatt cctaacgtct tgatgtatcg ggatatttgt
    13261 aaagtaactg gtgtcaagcg caaggtgatt ttgaagcgaa ccattttaat cagtttgctg
    13321 accctagtca tgtttctgtt aataggaacc atccagtggc tgttaggatt tttcttccaa
    13381 ccaagtggac gtttgtggag cttcttttat gtagctcttg tcggtgccat ggggggtgga
    13441 ctttatatgg ttatgagtct gcgtacctat ttgttagata aggtaatagg aaaagcccaa
    13501 gcagatcgcc tgcgagcaaa atttaagctt tcgtaaggta gcatttataa ataaaaggaa
    13561 taagttgaac aatcttttag gagaacttga aacttattcc ttttttagcg taaaaatagc
    13621 taaaattatc tttcaaaaat agaaaatgag gtttttttcg attttttgga aaaataattg
    13681 actaaacaaa ccaaacgttg tataataaaa caaatattta aagcaggagg ttctggaaat
    13741 gaaaaagtct aagagcaaat atctaacctt ggcaggtctt gtcctgggta caggagtttt
    13801 attgagcgcg tgtggaaatt ctagcacggc gtcaaaaacc tacaactatg tttattcaag
    13861 tgatccatct agcttgaact atctagcaga aaaccgcgca gcaacatccg atattgttgc
    13921 aaatttggta gacgggttat tagaaaatga ccaatatggg aatattattc catcattagc
    13981 agaggattgg actgtttctc aggacggttt gacctatacc tacaaacttc gtaaggatgc
    14041 caagtggttt acttctgagg gagaagaata tgcgcctgta actgcccagg attttgtgac
    14101 aggtttgcaa tatgcagctg ataaaaaatc agaagccttg tatctagtgc aggactctgt
    14161 tgctggtttg gatgactata tcactggtaa aacaagcgac ttttcaactg tcggtgtcaa
    14221 ggcacttgat gaccaaacgg ttcaatatac tttggttaaa ccagaacttt actggaattc
    14281 aaaaacactt gcaacgatac tttttcctgt taatgcagat ttcctgaaat caaaagggga
    14341 tgattttggg aaggcggatc catctagtat tttgtacaat ggacctttct tgatgaaagc
    14401 acttgtctca aaatctgcta ttgaatataa gaaaaaccct aattactggg atgctaagaa
    14461 tgtctttgta gacgatgtga aattgaccta ctatgatggt agcgaccaag aatcactgga
    14521 acgtaatttt acagctggtg cttatactac ggctcgtctt tttcctaaca gctccagcta
    14581 tgaagggatt aaagaaaaat acaaaaacaa tatcatatat agtatgcaaa attcaacttc
    14641 atatttcttt aattttaacc tagataggaa gtcttacaat tatacttcta aaacaagtga
    14701 cattgaaaag aaatcgactc aggaagcagt tctcaataaa aacttccgtc aggctatcaa
    14761 ttttgctttt gacagaacat cttatggggc tcagtctgaa gggaaagaag gtgcaacaaa
    14821 gattttgcgt aacctagtgg ttcctccaaa ctttgtcagt atcaagggaa aagactttgg
    14881 tgaagttgta gcctctaaga tggtcaacta tggtaaggaa tggcaaggta tcaactttgc
    14941 ggatggtcaa gacccttact acaatcctga gaaatccaag gctaagtttg cggaagctaa
    15001 gaaagaactc gaagcaaagg gtgttcaatt cccaatccac ttggataaga ctgtggaagt
    15061 aacagataaa gtaggcatac aaggagttag ttctatcaaa caatcaattg aatctgtttt
    15121 aggttctgat aatgtagtga ttgacattca gcaattaaca tcagatgagt ttgacagttc
    15181 aggctacttt gctcaaacag ctgctcagaa agactatgat ttatatcatg gcggttgggg
    15241 acctgattat caagacccgt caacctatct cgatattttt aatactaata gtggaggagt
    15301 tctacaaaat cttggactag agcctggaga agccaatgac aaggctaagg aagttggact
    15361 ggatgtctat actcaaatgt tggaagaagc taataaagag caagatccag ccaaacgtta
    15421 tgagaaatat gctgatattc aagcttgctt gattgatagt tctttagttc ttccaagtgt
    15481 ttcgcgtggg ggaacaccat cattgagaag aaccgtacca tttgctgctg cctatggttt
    15541 aaccggtaca aaagggattg aatcatataa atacctcaaa gtacaagata agattgtcac
    15601 aacagacgaa tatgcaaaag ctagagaaaa atggttgaaa gaaaaagaag aatccaataa
    15661 aaaagcccaa gaagaattgg caaaacatgt caaataaaat ctaattgtag ataagtttgt
    15721 gtaagaagtc tttgtgctta aaaacgcata atatcaggta tataaaatcc tagatgttat
    15781 gcgttttgtt ataggaagat ttattagatt ttatcctcaa attgagttat tcccgacttt
    15841 atccgttgaa atattataag gagtatttat gaaattaaaa ttattaagag tagatactaa
    15901 ggtgattatg gggagtttct tccttgttct gtctagtcta cttgctttgt tgcttcccct
    15961 tatcttaaaa ggtttaatag atgggagttc tattgaaaat ataggttcca aagtatttca
    16021 atcgtttttg atttttattg gtcaagcctt gttttcttct attggttact atctgtttag
    16081 tcaatcgggt gaaaaaaaga tagcaaaaat caggaaaaaa gtgatagagg ggttgattta
    16141 tgcagagaaa tccttctttg ataagagcca aagtggggag ttgacttctg ccattgtcaa
    16201 tgacatgagt gtcattcgtg agtttttaat tacgactttc ccaaatatta ttctgagttt
    16261 agttatggta cttggttcca ttgtagtctt atttagtctt gattggaatc tttctctact
    16321 tttattcatc actcttcctt gtatgatgtt tattatcttg cccctttcca atatcagtga
    16381 aaagtatagt cgtcgtttac aggaggaaat cggattttta acagggcaat tgactgaaaa
    16441 gattcaagaa catgaattaa ttaagactaa ccaagcagag aaaagtgtac aggatgtttt
    16501 ggataattgt attgaaaggg tacaaaacaa ttcactgaaa tcagataggg ttacttcttt
    16561 tgagaccccg tttgctctct tatttatctt tgcgactata gctgtgatgc taacctatgg
    16621 gggttatcgg gtcagcgcag gatatatatc tgtgggaacc ttggtttcgt ttttgattta
    16681 cctctttcaa ttacttaatc ctattagtaa tatagctaat tttgtaactg tttattctag
    16741 gagcaaggga tcttcagttg cactggagaa cttgcttgca gttcctaaag aaaaatttga
    16801 gggaggaaaa tcggtatcag gataaaggtt gaattttaac catgtctatt ttggttatga
    16861 tgaaaatcga cctgtcttaa aggatattac ttgttcaatt ttcaagggga aaaaaattgc
    16921 ttttgttgga ccatctggat caggaaaatc aacgattgtg cgtttgttag agcggtttta
    16981 taaaccgctt tcaggagata ttctaatgga gcaatcaagt atatatgatt ttaacttaaa
    17041 agaatggaga agtaaaatcg cttgggtttc acaaaataat gcagtcttat ctggcagtat
    17101 tcgtgacaat ctttgtctcg gtttgaatcg cttagtaact gatgatgaat tgatgaaagt
    17161 gctagactta gtatcactag gtgatgagat tcgctccatg aaagagggac tagatactga
    17221 agttggtgaa cgcggacgat tcttgtcagg ggggcaaagc caaagacttc aaatagctag
    17281 agcctactta aaagatgctg aaattcttat atttgatgaa gccactgcta atcttgatgc
    17341 ggattctgag tatgcgatta tcagtagcct ctattctgta ttaaaggaga agacggttgt
    17401 gattatagcg catagtttgt caacggtaaa agatgtggat tgtattttct tcttagagga
    17461 ggggaaaatc actggctcag gaactcataa ggaactactg gaaaatcatg agcgttatgc
    17521 tcgttttgtg caggagcaaa tgatagagtg aagtgtcttt tgagattcac cattttatag
    17581 tctattaaag ggagcaggaa aaactccctt tttatatagt ttgaaactat aactagctct
    17641 tgaaaagaag aaaatgagtt gatgaaaata agtggtacaa tagttactat agatttggag
    17701 gtattgtatg agcaaggaat tacacattaa cacaattttg gcccaggcgg gtattaagtc
    17761 agatgaagcg acaggtgcat tggtgacacc gcttcatttt tcaacgacct atcagcatcc
    17821 agagtttggt cgatctactg ggtttgacta tacgcgcact aaaaatccaa ctcgtagtaa
    17881 ggctgaggaa gtcttggcgg ctattgagtc agcagactat gccctagcga ctagctcagg
    17941 gatgtcagct attgtactgg cctttagcgt ctttccagta ggaagtaagg tcttggcagt
    18001 gcgtgatctt tacggtggtt cttttcgctg gtttaaccaa gtggagcagg aaggtcattt
    18061 ccattttacc tatgccaata cagaagaaga gttgattgcc gagttagaaa aggatgtgga
    18121 tgttctctat atcgaaaccc caaccaatcc cttgatgttg gaatttgata tcgaaaaact
    18181 agcaaaattg gctcatgcta agggtgccaa agtggtggtg gacaatacct tctacagccc
    18241 tatctaccaa cgtccgattg aagatggagc agatatcgtt ctccattcag caaccaagta
    18301 tctagcaggc cacaatgatg tcttggctgg agtggttgtg accaatagtt tagaactata
    18361 cgagaagctt ttttacaatc tcaatacaac aggggcagtc ttgtctccat ttgacagcta
    18421 ccagttgctt cgtggtctca agactttgtc tcttcgtatg gagcgctcaa cagctaacgc
    18481 ccaagaagtg gttgcctttt tgaaggattc tccagcagtt aaggaagttc tctacactgg
    18541 tcgtggaggc atgatttcct ttaaagtagc cgatgaaaca cgcattcctc atattttgaa
    18601 cagtctcaag gtcttctctt ttgcggaaag tttgggtgga gtggaaagtc ttattactta
    18661 tccaacgact caaactcatg ctgatattcc agcagaagta cgccattctt atggtttgac
    18721 agatgacctc ttgcgtttgt ctattgggat tgaggatgct agagatttga ttgcagattt
    18781 gcgccaagcc ttagaaggat aagacaaaga tgggaaaata tgattttaca agcctgccca
    18841 accgtttagg gcaccatacc tataaatgga aagaaacaga aacggatagt gaagttctac
    18901 cagcttggat agcggatatg gactttgtgg tcttgcctga aatccgccaa gccgtgcaaa
    18961 cttacgcaga ccaactggtt tatggttata cctatgccag tgaagactta attaaggaag
    19021 ttcaaaagtg ggaagctaca caatacggtt acaactttga caaagaggct cttgtcttta
    19081 tcgagggtgt ggtaccagcc atctcaacag ctattcaaac ctttacaaaa gaaggcgagg
    19141 cggttttaat taacacgcct gtctacccac cctttgctcg cagtatcaag ttgaataatc
    19201 gtagattgat tactaattcc ttagtggaaa aggatggtct gtttgagatt gactttgacc
    19261 aacttgaaaa ggatttggtg gaagaggagg ttaaactcta tattctttgc aaccctcaca
    19321 atcctggtgg acgtgtttgg gaaaaagaag tgttggagaa gattggccaa ctctgccaaa
    19381 aacacggtgt tttgttagtt tcggatgaga ttcaccaaga tttgaccctc tttggtcaca
    19441 aacaccagtc tttcaatacc atcaatcctg ccttcaaaaa ttttgctatc gtcttgagca
    19501 gtgccactaa aacatttaat attgctggaa caaaaaattc ctatgcagtc attgaaaatc
    19561 ctaagttgag actggctttc cagaaacgcc tgttggccaa taatcagcat gaaatttcag
    19621 gcttgggtta tttggcgaca gaagctgcct atcgatacgg taaagattgg ctagaggaac
    19681 tcaagcaagt ctttgaagac cacatcaatt atgtggtgga tctatttgga aaagagacta
    19741 aaatcaaggt catgaaaccg caaggtacct acttgatttg gcttgacttt tcagcttatg
    19801 acctgactga tgaaacattg caagagttgt tgagaaatga agccaaggtt atcctcaacc
    19861 gtggtttgga ttttggagag gaaggaagtc tccatgcccg catcaatata gctatgccca
    19921 aatctctgtt gcaagaagtc tgtcagcgga ttgtggctac ttttgccaaa cgttaaaaat
    19981 ctgaaagttc tataatgaag ttagccacct taccttagtc aagaattaga tgtttcacta
    20041 tgtttgagta agttgatgat ttcatttgat aacaggtttg aaactgttag gctaggtggc
    20101 caaggctaat catagccttg gtttagctga aaaaacaggt tcaagggttc ctgttgtcaa
    20161 atgaaatgtg atttaaggta taagaaaaca cctctgtgct atacttgttg ttcaccacaa
    20221 acacaaggaa aggcacagag atgcaagaac attatacccc aaaagggaaa catttgacaa
    20281 tagataaccg tcgcttgatt gagcggtgga agaatgaaaa taagtccaat cgtgaaattg
    20341 caggcttgtt aggaaaggcg cctcaaacga ttcatactga agtcaaaaga ggtacaactt
    20401 tacaacaagt gagaaaaggg ctatacaaaa aggtctattc tgccgattac gcacaaactg
    20461 tttaccaatt caatcgaaaa cggtcggtga aaaagttaat tttaacaaag gaaatcagag
    20521 agaagatctt acactatcat aagcaaaaat tttcgcctga aatgatggtt aacaagaagc
    20581 aagtgaaagt tggtatttca accatctact actggtttca taatggtcat ttaggattga
    20641 cgaaggccga catgctttat cccagaaaaa ggaaaggtgt caagaagcaa gctagtccga
    20701 actttaagcc ggcaggtaaa tctatcgaag aacgtcctga cgttattaat cttcgcttgg
    20761 aaaatggtca ttatgaaatt gataccgtcc tactgactaa gataaaaaat tattgcctgt
    20821 tagtcttaac cgaccggcgg agcagacacc aaattataag gttaattcca aataaaactg
    20881 ctgaatctgt caatcaggcg cttacgttac tattagggga gcatcgtatt ctgtccatta
    20941 ctgcagataa tggttcggag ttcaaacgat tgtctgaggt atttcctgag gaacatatct
    21001 actacgcaca tgcttactct tcatgggaga gaggttcaaa tgaaaatcat aatcgattaa
    21061 ttcggagatg gttacctaaa ggaaccaaga aaacgactcc gaaagaagta gcttttatcg
    21121 aaaattggat taacaactac cctaaaaaat gcttggacta caagtcgcca agtgaatt
//
LOCUS       DAVMEI010000017        20943 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVMEI010000017 DAVMEI010000000
VERSION     DAVMEI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.017"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            88..2310
                     /gene="recJ"
                     /locus_tag="VYQ24_000534"
     CDS             88..2310
                     /gene="recJ"
                     /locus_tag="VYQ24_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1967713.1"
                     /translation="MITPTYEWQFALQVENADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQILEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEVSFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMMDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2485..4146
                     /locus_tag="VYQ24_000535"
     CDS             2485..4146
                     /locus_tag="VYQ24_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1967714.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4215..4994
                     /locus_tag="VYQ24_000536"
     CDS             4215..4994
                     /locus_tag="VYQ24_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1967715.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5106..6326
                     /gene="murM"
                     /locus_tag="VYQ24_000537"
     CDS             5106..6326
                     /gene="murM"
                     /locus_tag="VYQ24_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1967716.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6331..7563
                     /gene="murN"
                     /locus_tag="VYQ24_000538"
     CDS             6331..7563
                     /gene="murN"
                     /locus_tag="VYQ24_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1967717.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7642..8343
                     /locus_tag="VYQ24_000539"
     CDS             7642..8343
                     /locus_tag="VYQ24_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1967718.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8383..10230
                     /gene="uvrC"
                     /locus_tag="VYQ24_000540"
     CDS             8383..10230
                     /gene="uvrC"
                     /locus_tag="VYQ24_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1967719.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGSLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQTKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            10220..11053
                     /locus_tag="VYQ24_000541"
                     /pseudo
     CDS             10220..11053
                     /locus_tag="VYQ24_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(11237..12037)
                     /locus_tag="VYQ24_000542"
     CDS             complement(11237..12037)
                     /locus_tag="VYQ24_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967720.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12208..12813
                     /locus_tag="VYQ24_000543"
     CDS             12208..12813
                     /locus_tag="VYQ24_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1967721.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12826..14226
                     /gene="pepV"
                     /locus_tag="VYQ24_000544"
     CDS             12826..14226
                     /gene="pepV"
                     /locus_tag="VYQ24_000544"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1967722.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGFKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14280..14858
                     /locus_tag="VYQ24_000545"
     CDS             14280..14858
                     /locus_tag="VYQ24_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1967723.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15018..15170
                     /locus_tag="VYQ24_000546"
     CDS             15018..15170
                     /locus_tag="VYQ24_000546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1967724.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            15213..15305
                     /locus_tag="VYQ24_000547"
     CDS             15213..15305
                     /locus_tag="VYQ24_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1967725.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15476..16801
                     /gene="brnQ"
                     /locus_tag="VYQ24_000548"
     CDS             15476..16801
                     /gene="brnQ"
                     /locus_tag="VYQ24_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1967726.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRVLTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16899..17936
                     /locus_tag="VYQ24_000549"
     CDS             16899..17936
                     /locus_tag="VYQ24_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1967727.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDTRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVIATERM
                     VDAYLKSEAVS"
     gene            17938..18399
                     /locus_tag="VYQ24_000550"
     CDS             17938..18399
                     /locus_tag="VYQ24_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1967728.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18417..19133
                     /locus_tag="VYQ24_000551"
     CDS             18417..19133
                     /locus_tag="VYQ24_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1967729.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19256..19681
                     /gene="rplK"
                     /locus_tag="VYQ24_000552"
     CDS             19256..19681
                     /gene="rplK"
                     /locus_tag="VYQ24_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1967730.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19889..20578
                     /gene="rplA"
                     /locus_tag="VYQ24_000553"
     CDS             19889..20578
                     /gene="rplA"
                     /locus_tag="VYQ24_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1967731.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKVKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         6295 a         3862 c         4855 g         5931 t
ORIGIN      
        1 tgttataaaa aagcaatctg gatagagaaa acgtctaaat catgttataa tgaagcaata
       61 gaattcttag aaagagtgga tgtctttttg ataacaccta cttatgaatg gcagtttgcc
      121 ctgcaggtag aaaatgcgga ttttacaaag atagccaaga aggctggact gggtcctgag
      181 gtggctcggt tattgtttga gagagggatt caggaccaag aaagtctgaa gaagttttta
      241 gaaccttcct tggaggactt acatgatgct tatctgctcc atgatatgga caaggcagtg
      301 gagcggattc gtcaggctat tgaagaaggg gaaaatattc tcgtttatgg agactatgat
      361 gcggatggca tgacttcggc ttctattgtg aaggaaagtt tggaacaact tggtgcggag
      421 tgccgagttt acctgccaaa tcgttttacc gatggctatg gccctaatgc tagtgtttat
      481 aaatacttta tcgagcaaga aggaatttcc ttgattgtga cggtggacaa tggggttgct
      541 ggtcatgagg ctattgcatt ggctcagtct atgggagtag atgtcattgt gacagaccat
      601 cattccatac ctgaaaccct gccagatgct tatgctattg tccatcctga acatccagat
      661 gcggattatc cttttaaata tttggctggt tgtggagttg ctttcaagtt ggcttgtgcc
      721 ctgttagaag aagtgcaagt ggaattgctt gatttggtcg ctattggaac tattgcagat
      781 atggtgagtc tgacggatga aaatcgtatc ttagttcaat atggtctgga aatgttgggt
      841 catacccagc gcattggtct gcaagaaatg ctggacatgg ctgggattgc tgctaacgaa
      901 gtaacagaag aaacggttgg tttccagatt gctcctcgtt tgaatgcctt gggtcgcttg
      961 gatgatccca atcctgccat tgatttgttg actggatttg atgatgagga agcgcatgag
     1021 attgccctta tgattcacca gaaaaacgaa gagcgcaagg aaatcgttca gtctatctat
     1081 gaagaagcca agaccatggt ggatcctgag aagaaggttc aggtcttggc caaggaaggc
     1141 tggaatcctg gggttctagg aatcgtggct ggtcgtttat tggaagaatt gggacagaca
     1201 gtcattgttc ttaatataga agacggtcgt gccaagggca gtgctcgtag tgtggaagcg
     1261 gtcgatattt ttgaagctct ggatccctat cgagacctct tcatcgcctt tggaggtcat
     1321 gcaggtgcag cgggtatgac gctggaagtt gagcaactct cagatttatc tcagattttg
     1381 gaagattatg ttcgtgaaaa aggtgcagat gctggtggca agaataagtt aaacctagat
     1441 gaagagttgg atttggaggc acttagcttg gaaacggtca aaagttttga acgtttagct
     1501 ccttttggaa tggataatca gaaacctatt ttttatatca agaattttca ggtcgaaagt
     1561 gctcgtacta tgggggcagg taatgcccat ctaaagctga aaatttccaa gggtgaggtg
     1621 agttttgaag tggtagcctt tggtcaaggc agatgggcga cagagttttc tcaaaccaag
     1681 aatctagagt tagcggttaa attgtctgtc aaccaatgga acggccaaac tgccctccag
     1741 ttgatgatga tggatgcgcg agtggaaggt gttcaacttt ttaacattcg tggaaaaaat
     1801 gcagtcttgc cagaaggtgt tccagtcttg gattttcctg gagaactgcc aaatcttgcg
     1861 gctagtgaag ctgttgtcgt aaaaaacatt ccagaggata ttactcagct gaagaccatt
     1921 tttcaggaac agcatttctc tgctgtctat ttcaaaaatg atattgacaa ggcctattat
     1981 ctgacaggtt atgggactag agatcagttt gccaaattgt acaagactat ttaccagttc
     2041 ccagagtttg atattcgcta caagctgaaa gatttggctg catatcttaa tattcaacaa
     2101 atcttgctgg tcaagatgat tcaagtattt gaagaactag gctttgtgac gataaaagat
     2161 ggtgtgatga cagtcaataa agaggcgcca aagcgggaga taggagaaag tcaaatttac
     2221 caaaatctca aacaaaccgt taaagaccaa gaaatgatgg cgctgggtac ggtgcaagaa
     2281 atttatgatt ttttgatgga aaaagagtag aagttaggaa agagttggga aatcaactct
     2341 tttttgaaaa cagaccttca ttttgaaaat catcaaaaaa atggtataat ggtaggaaaa
     2401 gattcggctg aaagtattag aacttttaga ataagagggt agagttgccc tataatcaag
     2461 ataaactaag attttggagg aaaaatgagt aatatcagtt taacaacact tggtggtgtg
     2521 cgtgagaatg gaaaaaatat gtacattgct gaaattggag agtccatttt tgttttgaat
     2581 gtagggttaa aatatcctga aaatgaacaa ttaggggtcg atgtggtgat tccaaacatg
     2641 gattaccttt ttgaaaatag cgaccgtatt gctggggttt tcttgaccca cgggcatgcg
     2701 gatgccattg gtgctctacc ttatctcttg gcagaggcta aagttcctgt atttgggtct
     2761 gagttgacca ttgagttggc aaagctcttt gtcaaaggaa atgatgccgt taagaaattt
     2821 aatgatttcc atgtcattga tgagaatacg gagattgatt ttggtgggac agtggtttcc
     2881 ttcttcccta cgacttactc cgttccagag agtctgggaa ttgtcttgaa gacatcggaa
     2941 ggaagcatcg tttatacagg tgacttcaaa tttgaccaaa cggctagtga atcttatgca
     3001 actgattttg ctcgtttggc agagattggt cgtgacggcg tcctggctct cctcagtgat
     3061 tcggccaatg cagacagcaa tattcaggtg gctagtgaaa gtgaagttag ggatgaaatt
     3121 acccaaacta ttgctgactg ggaaggtcgt atcatcgttg cagctgtttc cagtaatctt
     3181 tctcgtattc agcagatttt tgacgctgcg gataaaacag gtcgacgtat cgtcttgaca
     3241 ggatttgata ttgaaaatat cgtccgcaca gcgattcgtc ttaagaagtt gtctttagcc
     3301 aacgaaattc tcttgattaa gcctaaagat atgtctcgct ttgaagacca tgagttgatt
     3361 attcttgaga caggtcgtat gggtgaacct atcaatggac ttcgtaagat gtcgattggt
     3421 cgccatcgtt atgtagaaat caaggatggg gacctggtct atattgctac ggctccgtct
     3481 attgctaaag aagcctttgt tgcgcgtgtg gaaaatatga tttatcaggc aggtggggtt
     3541 gtcaaattga ttacccaaag tttacatgta tcagggcacg gaaatgtgcg tgatttgcag
     3601 ctgatgatca atcttttgca acctaagtac ctcttccctg tccaaggaga gtatcgtgag
     3661 ttggatgctc acgctaaggc tgccatggca gttgggatgt tgccagaacg catcttcatt
     3721 cctaaaaagg ggacgaccat ggcttacgag aatggagact ttgttccagc tggatcggtt
     3781 tcagcaggag atatcttgat tgatgggaat gccattggtg atgttggaaa tgttgttctt
     3841 cgtgaccgta aggtcttgtc agaggatgga attttcatcg tggctattac agtcaaccgt
     3901 cgtgagaaga aaattgtggc tagggctcgt gttcatacgc gtggatttgt ttatctcaag
     3961 aagagtcgcg atattctacg tgaaagttca gaattgatta accaaacggt agaagattat
     4021 cttcaaggag atgactttga ctgggcagat cttaaaggga aggttcgaga taatttgacc
     4081 aagtatctct ttgaccaaac caagcgtcgt ccagctattt taccagtagt catggaagca
     4141 aaataatcgt tgaaataaac agagagaaag tcgagtttcg gctttttctt atagaaaaat
     4201 agaaggagaa aatcatggca gtaatgaaaa tcgagtatta ctcacaagta ttggatatgg
     4261 agtggggggt gaatgtcctc taccctgatg ccaatcgagt ggaagaacca gagtgtgaag
     4321 atattcccgt cttgtacctt ttgcacggga tgtctggaaa tcataatagt tggcttaagc
     4381 ggaccaatgt agaacgcttg cttcgaggaa ctaatctcat cgttgttatg cccaatacca
     4441 gcaatggttg gtataccgat acccagtatg gttttgacta ctacacggct ctagcagagg
     4501 aattgccaca ggttctgaaa cgcttcttcc ctaatatgac tagcaagcgt gaaaagacct
     4561 ttatcgctgg tctttctatg ggaggctacg gctgcttcaa actggctctt gcgacaaatc
     4621 gtttttctca tgcagctagt ttttcaggtg ccctcagctt tcaaaacttt tctcctgaaa
     4681 gtcaaaatct gggaagtcca gcctactgga gaggtgtttt tggagagatt aaagactgga
     4741 caactagtcc ctattctctt gaaagtctgg ctaaaaaatc ggataaaaag accaaacttt
     4801 gggcgtggtg tggcgaacag gatttcttgt acgaagccaa taatctcgca gtgaaaaatc
     4861 tcaaaaaact aggttttgat gtgacctata gccatagcgc tggaactcac gagtggtact
     4921 actgggaaaa acaattggaa gtttttttaa caaccctacc aattgatttc aaattagaag
     4981 agagactgac ttagtttgaa cttcagcata gggggagtag aactaaaata aaatatgttt
     5041 tcactagact tttcaaacga aagtagtaga atagtaataa aatactggag gaaagagagt
     5101 aggaaatgta ccgttatcaa attggcattc ccacattaga atatgatcag tttgtcaaag
     5161 aacatgaatt agccaatgta ttacaaagta gtgcttggga ggaagttaag tctaattggc
     5221 aacatgagaa gtttggtgtt tacagggaag aaaaattact ggcgacagct agtattttga
     5281 ttagaactct tccgctaggc tataaaatgt tttacatccc aagaggacct atattggatt
     5341 atggggataa agaactcttg aattttgcca ttcagtctat taagtcctat gctcgcagta
     5401 agagagcggt ttttgtgact tttgacccaa gtatttgcct atctcaaagt ttaatcaatc
     5461 aggaaaagac agaatttcct gaaaatctgg ctattattga tagtttgcaa caaatgggag
     5521 taaggtggtc aggaaaaacg gaggaaatgg gagacaccat tcaacctcgt attcaggcga
     5581 aaatatacaa ggaaaatttt gaagaagata aactttccaa gtcaacaaaa caggctattc
     5641 gaacagcacg aaacaaaggg cttgagattc aatatggtgg actggaacta ttagattcat
     5701 tttcggagtt gatgaaaaaa actgagaagc gaaaagagat tcatttgagg aatgaagcct
     5761 attataaaaa attgttagat aattttaagg acaaggccta tatcaccttg gccaccttgg
     5821 atgtttctaa acgttcgcaa gagttagaag aacagttagc gaaaaataga gccttggaag
     5881 agacctttac tgagtcgact cgaacttcaa aagtagaagc gcagaagaag gaaaaagaac
     5941 gtttgttaga ggaattgacc ttcttgcagg aatatataga tgtaggtcaa gcgagagttc
     6001 ctttagcggc tactttgagt ttggaatttg gtactacctc tgtcaatata tatgctggta
     6061 tggatgatga ttttaaacgt tacaatgcac caattttaac ttggtatgaa acggctcgct
     6121 atgcctttga acgaggtatg atctggcaaa atttaggtgg tgttgaaaac tctctcaatg
     6181 gtggacttta tcattttaag gaaaaattta atccaacgat tgaagaatac ttgggtgaat
     6241 ttacaatgcc cactcatcct ctctatcctc tgttaagact tgctcttgat ttccgtaaaa
     6301 cattaagaaa aaaacataga aagtaagtat atggcactaa caacactcac gaaagaagag
     6361 tttcagactt attctgatca ggtttcttct cgttccttta tgcaatctgt ccagatgggg
     6421 gatttgctag aaaaaagagg ggctcgaatt gtttatcttg ctttgaaaca agaaggagaa
     6481 attcaagttg cagctctggt ttatagcctg cccatgctgg gtggtctgca tatggaactc
     6541 aattcggggc cgatttatac ccaacaagat gctcttccag ttttttatgc agagttaaaa
     6601 gaatatgcca agcaaaatgg tgtattagag ttgcttgtaa aaccctatga aacttatcaa
     6661 acttttgata gccaaggtaa tccaatagat gctgagaaaa aaagtattat tcaagatttg
     6721 actgatttag gttatcaatt tgatggctta acaacaggtt acccaggtgg agaaccagat
     6781 tggttatact ataaagattt aactgaatta actgaaaaga gtttgcttaa aagttttagc
     6841 aaaaagggta aacccttggt gaaaaaggct gaaacctttg gcattcggtt gaaaaagtta
     6901 aaacgtgaag aactatcgat ttttaagaat ataacaaaag aaacctctga acgtagagaa
     6961 tatagtgata aaagtttaga atattatgag catttttatg atacttttgg agaacaagcg
     7021 gagtttctca tagcaagctt aaatttttcg gactatatga gcaaattgca aggtgaacaa
     7081 agtaaactag aagaaaactt ggacaagttg cgacttgatt tgagtaaaaa tcctcattct
     7141 gagaaaaaac aaaatcaact gagagaatat tctagtcaat ttgaaacgtt tgaagttcga
     7201 aaagcagaag cgcgagactt gattgaaaaa tatggagaag aagatattgt tttagctggg
     7261 agtttatttg tttatatgcc tcaggaaacg acttatctct ttagtggttc ctacactgag
     7321 tttaataagt tctatgcccc tgcactgctt caaaaatatg ttatgttgga aagcataaaa
     7381 cgtggaatac ctaaatacaa cttcctaggc attcaaggga tttttgatgg aagtgatggt
     7441 gttttgcgtt ttaaacagaa ttttaatggc tatattgtac gcaaagcagg tactttccgt
     7501 taccatccat cgcctttaaa atacaaagct atccagttac tcaaaaaaat agtaggacgt
     7561 taagatgaaa aagtcagtat ttagatttct tttagcttct tttagtaaaa taattcttat
     7621 ttgctagaaa ggtggagaga catgcgctgg ctttttcgtt tgataggggc tttcttttct
     7681 tttgtgtggc gtttgttttg gcgtctggtt tggatagttg tgctcttatg tgtgcttgct
     7741 ttcggacttc tctggtatct gaacggagat tttcaaggag cgctaaagca agcagaacgg
     7801 tcagtaaaaa ttggtcaaca aagtattgac caatgggaga aaacagggca actgcctaag
     7861 ttaagccaga cagatagtca ccagcattct gaaggaaggt gggcacaggc ctctgctcgt
     7921 atttacctgg atccgcagat ggattcacgc tttcaagagg cttatttaga agcaatccag
     7981 aactggaatc aaactggtgc ttttaacttt gaactcgtga ctgagtctag taaggcggat
     8041 attacggcta cggagatgaa cgacggaggc actcctgtgg caggagaggc ggaaagtcaa
     8101 actaatctct taacagggca attcttgtcc gtaacggtgc ggttgaatca ttattatttg
     8161 tccaatccat actatggcta ctcctatgaa cgccttgtcc atacggcaga acatgagtta
     8221 ggtcatgcga ttggcttgga ccatacagat gagaagtctg tcatgcaacc agcaggttcc
     8281 ttttatggta tccaggaaga ggatgttgca aacctccgaa aaatatatga gactagtgag
     8341 tagggtacta tctttcccta ctttttttgc tataatggaa ctatgaacaa cttgattaaa
     8401 tcaaaactag agctcttgcc gaccagccct ggttgctaca ttcataagga taaaaatggc
     8461 accattatct atgtaggaaa ggctaaaaat ctgcgtaatc gagtacggtc ctattttcgt
     8521 ggaagtcatg ataccaagac agaggctctg gtgtctgaaa ttgtggattt tgaatttatt
     8581 gttacggagt ctaatattga ggcacttctc ctagaaatca acctgatcaa ggaaaacaag
     8641 cccaagtaca atatcatgct caaggatgac aagtcctatc ctttcatcaa aatcaccaat
     8701 gagcgctatc cacgcttgat tatcactcgt caggtcaaaa aggacggaag tctttatttt
     8761 ggaccctatc ccgatgtggg ggcagccaat gaaatcaagc ggttgctgga tcggatattc
     8821 ccttttcgta agtgtaccaa cccgccctct aaggtctgtt tttattacca tatcggtcaa
     8881 tgcatggccc acaccatctg taagaaggat gaggcttatt tcaagtctat ggcccaggag
     8941 gtttctgatt ttctgaaagg gcaggataac aaaatcattg atgagctaaa gggcaaaatg
     9001 gcagcggcag cccagactat ggagtttgaa cgtgcggcgg aataccgtga cctgattcag
     9061 gctattggaa cgcttcgaac caagcaacgg gtcatggcta aggatctcca aaatcgcgat
     9121 gtctttggct actatgtgga taagggctgg atgtgtgtgc aggttttctt tgtccgtcag
     9181 ggtaagctca tcgagcgcga tgtcaatctc ttcccctact tcaatgatcc agatgaggat
     9241 tttttgacct atgtaggaca attctatcaa gaaaaatccc atctagttcc caatgaggta
     9301 ctgattccgc aagatattga cgaagaggct gtcaaggcct tggtggattc caagattctt
     9361 aagcctcaac gtggagagaa aaagcagctg gtcaatttag ctataaaaaa tgcccgtgtt
     9421 agcctagagc agaagttcaa cctgctagag aaatctgtcg agaagaccca aggagctatt
     9481 gaaaatctag gacgcttgct tcaaatccca accccagttc gcatcgagtc cttcgataac
     9541 tccaatatca tgggaactag tcctgtttca gccatggtgg tctttgtcaa cggcaagcct
     9601 agtaagaagg attaccgtaa gtataaaatc aagaccgtag tcgggccaga cgactatgcc
     9661 agtatgcgag aggtcattcg cagacgctat ggtcgagtac agcgtgaggc tttgactcct
     9721 ccagatttga ttgtgattga tggggggcaa ggtcaagtca atattgctaa gcaagtcatc
     9781 caagaagaac tgggcttgga tattccaatt gcaggtctgc aaaagaatga taagcaccaa
     9841 acccatgaat tgctctttgg agatccgctg gaagtggtgg acttgtctcg caattctcag
     9901 gaatttttcc tcctccaacg cattcaagat gaggtccacc gctttgccat taccttccac
     9961 cgccaactgc gctccaaaaa ttctttctca tctcaattgg atgggattga tggtctggga
    10021 cctaaacgca agcaaaatct catgaggcac ttcaagtctc tgaccaaaat caaggaagcc
    10081 agtgtggatg agattgtcga agttggagta ccaagagcag tagcagaggc tgtgcaaacc
    10141 aagttgaacc cgcaggaaac agaaatcttg cttcaagtgg cggaagaaag agtagattac
    10201 caaacggaag gaaaccacaa taaaccataa aatcgcaatt ttatcagatg ttcatggcaa
    10261 tgcgacggcg ctagaagcag tgattgcaga tgctaaaact caaggagcca gtgaatattg
    10321 gctcatggga gacattttcc tccctggtcc aggtgcaaat gacttggtcg ctctgttaaa
    10381 ggaacttcct atcacggcaa gtgttcgagg caattgggat gattgtgtcc ttgaggcttt
    10441 agatgggcaa tatggcttag aagacccaca ggaagttcag ctcttgcgta tgacacagta
    10501 tttgatggag cgaatggatc ctgcaacgat tgtctggcta cgaagcttgc ctttgctgga
    10561 aaagaaagaa attgacggat tgcgcttttc tatctctcat aatttacctg acaaaaacta
    10621 tggtggtgac ttgctagttg agaatgatac agagaaattt gaccaactgc tagatgcgga
    10681 aacggacgtg gcagtttatg gtcatgttca caagtagttg cttcgttatg gtagtcaagg
    10741 gcaacaaatc atcaatccag ggtcgattgg catgccctat tttaattggg aggcgttaaa
    10801 aaatcaccgt gcccagtatg ccgtgataga ggttgaagat ggggaattgg taaatatcct
    10861 atttcgtaaa gtcgcttatg attacgaggc tgagttagaa tttgccaaat ccaagggttt
    10921 tccctttatc gaaatgtatg aagaactacg tcgtgaagat aactatcaga ggcacaatct
    10981 ggaactatta gcaagcttaa tagaaaagca taggtatgta gaggatgtga agaatttttt
    11041 tgattttttg taagagtttc ctaaaataac caatgcaaac taaaaaagcg attggctgat
    11101 cccatcgctt ttaatatatc ttatactcaa tatgaaaatc aaagagcaaa ctaggaagct
    11161 agccgtaggt tgctcaaagc acagctttga ggttgcagat aaagctgacg tggtttgaag
    11221 agattttcga agagtgttat tgtaactgag attgatctgg gaggtaagaa ccacctagat
    11281 aggtattgct gagtttttca agggttccgt cttgatagag ttctttgagc gctttatcaa
    11341 attgctcttt aaactctttt tggtcgcttg agaaaatgat ataattgctg gggctatctg
    11401 cagaaggtaa atcaacgact gagaggtcta aaccacggtc cttgataatc ttttgaacgg
    11461 ataccttgtc aaaaactagg aaatcaaact ctccgttagc aaggtctagg attcgtttac
    11521 caatatcctc accagaaaaa ttaattgtag cgggattatc agtgtgtttc tgattccagt
    11581 tattgatgaa ttgagcgtta gaagttccgg tatcctcttg tgttgtttta ccagcgatct
    11641 ggtcaagaga agtcaaagga tttttcttgt tgctgacaag gacgagggga ttgttggaaa
    11701 tcggaagcga gtaaaggtat ttttcagcac gctcttttgt gtaactcaaa ttattggccg
    11761 cagcctgata gtgaccagaa tcaagtcctg ggaagatact ttcccaggcg gttctttgaa
    11821 attgaatctc gtagtcgctg agtttttcat ctactgcctt tagaacttcg atatcgaagc
    11881 ctgtcagatt gcctttatct tcgtagtcaa atggtggcac gtcgccagct gtagcaagga
    11941 cgattgtctt ttgagcgcta gtctctttgg gtgtagcttg attcccacag gcaaccaaaa
    12001 atggtaggat agctagtaat aggctaaatt ttttcatact gtctccattc aaatgtaaag
    12061 tacttgctag tttatcagaa actttcctga taaaccaata tatatctttt atgtctgtga
    12121 tagaaaaact taatcatgaa aaatccctgc aagctctttc aaattatggt agaatggaag
    12181 cagtagaatt agaaaaggaa atcgattatg aaatttcttg aattaaataa aaaacgtcat
    12241 gcgaccaagc atttcactga taagcctgtt gatccaaaag atgtgcgtac ggctatcgaa
    12301 attgcaacct tggcgccaag cgcccacaac agccagcctt ggaaatttgt agttgttcgt
    12361 gagaaaaatg ctgaactggc aaagttggct tatggttcaa actttgagca ggtatcatca
    12421 gcacctgtaa ccattgccct atttacagat acagaccttg ccaaacgcgc tcgtaagatt
    12481 gcccgagttg gtggtgctaa caatttctca gaagaacaac ttcaatactt catgaaaaat
    12541 ttgccagctg aatttgcacg ctacaatgaa caacaagtca gcgactacct agctctcaat
    12601 gcaggtttgg ttgccatgaa cttggttctt gcattgacag accaaggaat tggttctaac
    12661 attattcttg gttttgacaa atcaaaagtt aatgaagttt tggaaatcga agaccgtttc
    12721 cgcccagaac tcttgatcac agtgggttat acagacgaaa aattggaacc aagctaccgc
    12781 ttgccagtag atgaaatcat cgagaaaaga tagaaagaag aaaaaatgac agcaattgat
    12841 tttacagcag aagtagaaaa acgcaaagaa gacctcttgg ctgacttgtt tagccttttg
    12901 gaaatcaatt cagaacgtga tgacagcaag gctgatgccc agcatccatt tgggcctggt
    12961 ccagtaaaag ccttggagaa attccttgaa atcgcagacc gcgatggcta cccaactaag
    13021 aatgttgata actatgcagg acattttgag tttggtgatg gagaagaagt tctcggaatc
    13081 tttgcccata tggatgtggt gcctgctggt agcggttggg acacagaccc ttacacacca
    13141 actatcaaag atggtcgcct ttatgcgcgc ggggcttcgg acgataaggg tcctacaaca
    13201 gcttgttact atggtttgaa aatcatcaaa gaattgggtc ttccaacttc taagaaagtt
    13261 cgcttcatcg ttggaacaga cgaagaatca ggctgggcag acatggacta ctactttgag
    13321 cacgtaggac ttgccaaacc agatttcggt ttctcaccag atgctgaatt tccaatcatc
    13381 aatggtgaaa aaggaaatat cacggaatac ctccactttg caggagaaaa tacaggtgtt
    13441 gcccgtcttc acagctttac aggtggttta cgtgaaaata tggtaccaga atcagcaaca
    13501 gcagtcgttt caggtgactt ggctgacttg caagctaaac tagatgcctt tgttgcagaa
    13561 cacaaactta gaggagaact ccaagaagaa gctggcaaat acaaggtgac gatcattggt
    13621 aaatcagccc acggtgctat gcctgcttca ggtgtcaatg gcgcaactta ccttgccctc
    13681 ttcctcagtc agtttggctt tgctggtcca gccaaagact accttgacat cgcaggtaaa
    13741 attctcttga acgatcatga gggtgaaaat cttaagattg ctcatgtgga tgaaaagatg
    13801 ggtgctcttt ctatgaatgc cggcgtcttc cgcttcgatg aaacaagtgc tgataatacc
    13861 attgccctca acatccgcta tccaaaagga acaagtccag aacaaatcaa gtcaatcctt
    13921 gaaaacttgc cagttgtttc tgttagcctg tctgaacacg gtcacacgcc tcactatgtg
    13981 ccaatggaag atccacttgt gcaaaccttg ttgaatatct atgaaaaaca aactggcttt
    14041 aaaggtcatg aacaagtcat cggtggtgga acctttggtc gcttgttaga gcgcggagtt
    14101 gcctatggtg ctatgttccc agactcaatt gataccatgc accaagccaa tgaatttatc
    14161 gccttggacg atctcttccg agcagcagca atctatgccg aagctattta cgaattgatc
    14221 aaataaaacg atagaagtct gagatcttat gcttggactt ctttttggag ggaaagtaga
    14281 tgtctcaaat cgaaagaatc aaacaggcta tcatggcgga ttcacagaat gccagctata
    14341 cagagcgtgg cattgagcct ctctttgcag cgccaaaaac tgctcgcatc aatatcatcg
    14401 gtcaggctcc gggacttaaa actcaagaag caggccttta ctggaaagat aaaagtggtg
    14461 accgcttgcg ggactggcta ggtgtggatg aagatacctt ttacaattca ggttattttg
    14521 ctgttttgcc tatggatttc tactttccag gacatggcaa gtcgggtgat cttccgcctc
    14581 gtacaggttt tgcagaaaaa tggcatccgc agctcttaca ggaattgcct gatattcagt
    14641 taaccctctt gattgggcaa tatgcccaag cctactattt acaggagaaa atcagtggga
    14701 aggtaacgga gagggtgaaa cactataaag actatctgcc agcctatttt ccgctagttc
    14761 acccatcacc acgaaatcaa atctggatgg ccaaaaatcc ttggtttgag gcagaagtag
    14821 tgccagattt gaaaaaaaga attaaaacca ttttatagtc aatgaaaatc aaagagcaaa
    14881 ctaggaagct agtcgtaggc tgctcaaagt acagctttga ggttgcagat aaaactgacg
    14941 aagtcggtaa catacgcacg gtaaggcgac gctgacgtgg tttgaagaga ttttcgaaga
    15001 gtattagaag aaaaagaatg aaagaaatag cctttgacgc attttaccag ctttaccaaa
    15061 acgaccagct ttctctagtg gatgtgagag aagtggatga gtttgcagct cttcatttag
    15121 aatgtgccca caacctaccg cttagtcaat tggctgatag ttatgattaa ttggacaaag
    15181 atcgcttgca ttatattatt tgcaaatctg gaatgagatc ggcgcgtgct tgccaattcc
    15241 tgttagaaca aggttataat gttatcaatg tccagggtgg catgttagcc tttgaagaac
    15301 tttaaaattt tgcatttctc ctacttggtg tggactgggt aggagagttt tatttttaga
    15361 taattcttat ttttaagaaa attgaaaaca tttaatattt gcctcgtgat gcttttttca
    15421 gactcctaat cgtggtatac taggtcagta ttttataaat atgaaggaga tttttatggc
    15481 taaaaaaggt accctaacag gtttgctcct gtttggaata ttttttggtg cggggaactt
    15541 gatttttccg ccttctctag gtgctctatc tggagaacat tttcttcctg ccatcgcagg
    15601 ttttgtcttt tcaggcgttg gtatcgccgt cttgaccctt attattggaa cgctaaatcc
    15661 taaaggatat atctacgaga tttcaacgaa gatagcgcct tggtttgcga ctctttacct
    15721 ctcagttctt tacttgtcaa tcggtccatt ctttgctacc ccacgtactg ctacaacagc
    15781 ttacgaagta gggattagcc cccttttgtc ggatgcaaat aaaggacttg gcttgattgt
    15841 atttacggtt ctgtattttg cggcagccta tttgatttcg cttaatccat caaaaatctt
    15901 agaccgcatt ggacgtgttt taacgccagt ctttgcaatt ttgattgtta tcttggtcgt
    15961 tctgggagct atcaaatatg gtggaacaag tcctcaagct gcttcagctg cttatcaagc
    16021 ttctgccttt ggtacaggtt tcctagaagg ttacaatacc ttggacgccc ttgcttcagt
    16081 ggcctttagc gtaatcgcag ttcaaacctt gaaacaactt ggattttcaa gtaagaaaga
    16141 atacatttca actatttggg ttgttggtat cgttgttgcc cttgccttca gcgctcttta
    16201 catcggttta ggttttcttg gaaatcattt cccagtacca gctgaagcga tgaagggtgg
    16261 aacaccaggt gtttacatct tgtcacaagc cactcaagaa atctttggct caacagctca
    16321 actcttcctt gcagctatgg ttaccgtaac ctgcttcaca acgactgttg gtttgattgt
    16381 gtcaacagct gagttcttta atgagcgctt cccacaaatc agctacaagg tttatgcgac
    16441 agcctttacc ttgattggat ttgctattgc caatttgggt cttgatgcga ttatcaagta
    16501 ctcaattcca gtactggtta tcttgtaccc aatcacgatt gctatcgtta tgattgtcat
    16561 tgtcaacaaa tttgttgccc tttcaaaacc aggtatgcag ttgacaattg ctgtggttac
    16621 agttattgcc attgcaagcg tactaggaag ctcgtttaag gttgagtttc ttgcaaatct
    16681 tgttagcgtt cttccttttg ccaaggcatc tctcccatgg ttggtgccag ccattgttgg
    16741 aatcttgctc tcattggttc taccaaacaa gcaagaaagc gatgtttttg aaatggaata
    16801 atcacttaaa tcacttttgt agccaagtct acaggagtga ttttcttttt ttatccgatg
    16861 ataaatgtgt tataataggt agcgaaagag gtgaagaaat gaatcaaaca gtagaatata
    16921 tcaaagaact gacagccatc gcatcgccaa cgggcttcac tcgtgaaatt tcgaattatt
    16981 tagtcaagac tctagaaggt tttggttacc agccagttcg cacagctaag ggtggtgtca
    17041 atgtgaccat caaaggtcaa aatgatgagc aacatcgcta tgtgactgcc catgtggata
    17101 cgctgggtgc tattgttcgt gctgtcaaac cagatggtcg tcttaaattg gaccgtatcg
    17161 gtggttttcc ttggaacatg attgaaggtg aaaactgtac cgttcatgtg gctagcacag
    17221 gtcaaaaggt atcaggaacc atcctcatcc accaaacttc ttgccatgtc tacaaggatg
    17281 caggaactgc agaacgcacg caggacaata tggaagtgcg tttggatgta aaagtaagca
    17341 atgaaaaaga aactcgtgct cttgggattg aggtcggtga ttttatcagc ttcgatacac
    17401 gaactgttgt gactgataca ggttttatca agtctcgtca tttggatgat aaggtcagcg
    17461 cagcgatttt gctcaacctg cttcgtattt ataagaaaga gaagattgaa ttgccagtaa
    17521 caactcattt tgctttttca gtttttgaag aagtaggaca tggtgctaat tctaacattc
    17581 ctgctcaggt agtagaatat ctggctgtgg atatgggagc tatgggagat gaccagcaga
    17641 cagatgagta cacagtatct atctgtgtca aggatgcttc aggtccttat cactatgact
    17701 tccgtcaaca cttggttgcc ttggctaaag agcaagatat tccatttaag ctcgacatct
    17761 atccatttta cggatcggac gcttcagcag cgatgtcagc aggtgcagag gtcaagcatg
    17821 cacttcttgg agctggtatt gagtccagtc actcttatga gcgcacccac atcgattcag
    17881 taatagcgac tgagcgtatg gttgatgctt atcttaagag cgaggcggta tcatagaatg
    17941 tgcttaatct gtcagagaat cgaactcatt aaagctgggc aaaatcccta ctttgtaaaa
    18001 gaactagaaa caggctatgt tgttattggt gactaccaat actttaaggg ctatacctta
    18061 tttttagcaa aagatcatgt cacagaactc catcatatgg aaacttctgt aaaacttcgc
    18121 tttttagagg aaatgagttt agtccaagaa gcagtcgcca aagcatttga ggctgaaaag
    18181 atgaatattg aactcctagg aaatggcgat gcccacgctc attggcatct ttttccccga
    18241 agagcaggtg atatgaagag tcacggtttg aatggccgtg gcccagtctg gtgggtgccc
    18301 tgggaagaaa tggcggcaga agattgccaa gtacaatcgc cagagctgga agagatgatt
    18361 aaaatcttat ctcatgaatt ggagaagtat ttggcctaag agagaggaag aaagaaatga
    18421 aaaaaagata tgtcatttta tctggtttac tagcattgac ccttgcagct tgttcgcaag
    18481 aaaaaaccaa agttgaagaa aacactcaaa aaacggagca aagcagtcaa ccggaaggaa
    18541 ctgtaggtag caaatctcaa gcttctagtc agaaaaaggc agaagttagc aataaaggga
    18601 gctattatag tatccaaggg aaatacgatg agattatcct agctaataaa cgttatcctt
    18661 tgtcaaaaga ttacaatcca ggagaaaatc caacagccaa ggcagagttg ctcaaactaa
    18721 tcgcagctat gcaggctgag ggctatccaa tcagtgacca atacagtggg tttaggagtt
    18781 atgaaactca ggctaagctc taccaagact atgtcaatca agatggtaag gaagcagcag
    18841 atcgctactc agcacgccca ggttacagtg aacaccagac gggtcttgct tttgatttga
    18901 ttgggacaga tggggattta gttactgaag aaaaggcagc ccagtggctc ttggaccatg
    18961 cggctgacta tggctttgtt gttcgctatc ttaaaggcaa ggaaaaggag actggctaca
    19021 tggcagaaga atggcatctc cgctacgttg gaaaagaagc caaagaaatt gctgcaagtg
    19081 gtctcagttt ggaagagtac tatggctttg aaggcggcga ttatgtcgat taaaaatgaa
    19141 agttttctct tgcattttta gataaatagt gtataatgga aaggtatgtg taaagcatac
    19201 ttgtgggagg taaaaatctc taattaccgc caaaaccaca aaggaggatt taaaaatggc
    19261 taaaaaagtc gaaaaacttg taaaattgca aatccctgct ggtaaagcta caccagctcc
    19321 accggttgga cctgctcttg gtcaagctgg tatcaacatc atgggattca caaaagagtt
    19381 caacgctcgt acagctgacc aagctggtat gatcattcca gttgttatct cagtttacga
    19441 agataaatca tttactttcg ttacgaaaac accaccagct gctgttcttt tgaaaaaagc
    19501 tgcaggtgtt gaaaaaggat caggtacacc taataaaact aaagttgcta cagttactcg
    19561 tgcacaagta caagaaattg cagaaactaa gatgccagat ttgaacgcag caaacgtaga
    19621 gtctgcaatg cgtatgatcg aaggtactgc tcgttctatg ggattcactg ttgttgacta
    19681 atcaataaca cagtagaaaa tctcacagca gtctattagt tgcttttcat actaggcaag
    19741 tgactgaggc ttgtacttgg atacagcaag ggagcttaag gccgtaggaa gagaaaaata
    19801 gtagactgaa aacccgcaag acttcatcat ttcgagaagt gacgtgggag atgaaaatcg
    19861 attgaaccac ttacaaggag aatagaaaat ggctaaaaaa agcaaacaac ttcgtgctgc
    19921 tcttgagaaa atcgacagca caaaagcata cagtgtagaa gaagctgtag cacttgcaaa
    19981 agaaactaac tttgcaaaat ttgatgcaac tgtagaagtt gcttacaact tgaacatcga
    20041 cgttaaaaaa gctgaccaac aaatccgtgg agcaatggta ttgccaaacg gtactggtaa
    20101 aacttcacgt gttcttgttt tcgcacgtgg tgcaaaagct gaagaagcaa aagctgctgg
    20161 tgcagacttt gttggtgaag atgaccttgt tgctaaaatc aacgacggtt ggttggactt
    20221 cgacgtagtt atcgctacac ctgatatgat ggctcttgtt ggacgtcttg gacgtgtcct
    20281 tggaccacgt aacttgatgc caaaccctaa aactggtact gtaacaatgg atgttgctaa
    20341 agcagttgaa gagtctaaag gtggtaaaat cacttaccgt gctgaccgtg caggtaacgt
    20401 tcaagcaatc atcggtaaag tatcatttga agctgaaaaa ttggttgaaa actttaaagc
    20461 tttcaacgaa acaatccaaa aagtaaaacc agctacagct aaaggaactt acgtaacaaa
    20521 cttgactatc acaactactc aaggtgttgg tatcaaagtt gacgtaaact cactttaatc
    20581 agtagttaaa gtaatgtaaa aaagttgaag acactatgtc tcaacttttt ttgatgtacg
    20641 acgggcatgt cgtatatctg aggtgtaagt cctcggtggg cacctactac cggtgaaccc
    20701 aatagcgatt cccaagtctg acgctggaaa taagaatcgt cagaggaagg gatagcgaaa
    20761 tcgtggctct acgaacagga acgtgatagt aaggcctata tagcggataa ggtatctggc
    20821 tggggcgacc actatcaatt ataataaaat gaaataagaa taggacgaat cgttcaggac
    20881 agtcaaatcg atttctaaca atgttttaga agtagaggtg tactattcta gtttcaatct
    20941 act
//
LOCUS       DAVMEI010000018        20734 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVMEI010000018 DAVMEI010000000
VERSION     DAVMEI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.018"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            210..2192
                     /locus_tag="VYQ24_000554"
     CDS             210..2192
                     /locus_tag="VYQ24_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967732.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKAEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAVVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGGITDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2493..7802
                     /locus_tag="VYQ24_000555"
     CDS             2493..7802
                     /locus_tag="VYQ24_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1967733.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDTDNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDASTVEKPEFKLSSLASDEDKAPAPELKQE
                     VTKRETAEQSLPATGESQSDTALFLAGVSLALSALFVAKTKKD"
     gene            complement(7970..10135)
                     /locus_tag="VYQ24_000556"
     CDS             complement(7970..10135)
                     /locus_tag="VYQ24_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1967734.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGSRHQSSNVSFGINQAVETNRDWGSAMKPITDYAPALEYDIYDSTAS
                     IVHDVPYNYPGTDTPLYNWDKVYFGNITIQYALQQSRNVTAVETLNKVGLDRAKTFLN
                     GLGIDYPSMHYANAISSNTTESNKKYGASSEKMAAAYAAFANGGIYHKPMYINKIVFS
                     DGSSKEYADPGTRAMKETTAYMMTEMMKTVLAYGTGRGAYLPWLPQAGKTGTSNYTDD
                     EIENYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIVGDGFYVAAKVYRSMMTY
                     LSEDNNPGDWTMPEGLYRSGEFVFKKGARSAWTAPAPQQAPTPESSSSTSESSTSQSS
                     STTPSTNNSANNNTNNQQPNTTPGQQNQNQNQNPQPAQP"
     gene            complement(10132..10728)
                     /gene="recU"
                     /locus_tag="VYQ24_000557"
     CDS             complement(10132..10728)
                     /gene="recU"
                     /locus_tag="VYQ24_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1967735.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10794..11321
                     /locus_tag="VYQ24_000558"
     CDS             10794..11321
                     /locus_tag="VYQ24_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1967736.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11391..11720
                     /gene="gpsB"
                     /locus_tag="VYQ24_000559"
     CDS             11391..11720
                     /gene="gpsB"
                     /locus_tag="VYQ24_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1967737.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11741..12121
                     /gene="rnpB"
                     /locus_tag="VYQ24_000560"
     ncRNA           11741..12121
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYQ24_000560"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12206..13363
                     /locus_tag="VYQ24_000561"
     CDS             12206..13363
                     /locus_tag="VYQ24_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1967738.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13375..14769
                     /gene="mapZ"
                     /locus_tag="VYQ24_000562"
     CDS             13375..14769
                     /gene="mapZ"
                     /locus_tag="VYQ24_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1967739.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14845..16269
                     /gene="gndA"
                     /locus_tag="VYQ24_000563"
     CDS             14845..16269
                     /gene="gndA"
                     /locus_tag="VYQ24_000563"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1967740.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVYASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16281..16970
                     /locus_tag="VYQ24_000564"
     CDS             16281..16970
                     /locus_tag="VYQ24_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1967741.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17068..18084
                     /gene="cbpC"
                     /locus_tag="VYQ24_000565"
     CDS             17068..18084
                     /gene="cbpC"
                     /locus_tag="VYQ24_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1967742.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMATGWYQDGSTWYYLNASNGGMKTGWFQVNGKWYYAYGSGALAVNTTVDGYSVNYNG
                     DWVR"
     gene            complement(18203..19435)
                     /locus_tag="VYQ24_000566"
     CDS             complement(18203..19435)
                     /locus_tag="VYQ24_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1967743.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLAMLSIIIS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(19446..19898)
                     /locus_tag="VYQ24_000567"
     CDS             complement(19446..19898)
                     /locus_tag="VYQ24_000567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967744.1"
                     /translation="MFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCEGISGIILY
                     LCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVI
                     LACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNKFPFDVQT"
BASE COUNT         6501 a         3738 c         4617 g         5878 t
ORIGIN      
        1 caaaaatata gaaaaatcgg tttttagtga accctttttt ggtataaact ttgttagtga
       61 agcgcaagag agccgaatag ttattacttt agaatatcct taaaagtatt tttcagaatt
      121 taccaaatta attaaaaaat tcagaaaatt atattgacat ctctctgaaa agagtctata
      181 atagagagaa agttttaaag gagaagatga tgaaaagttc aaaactattt gcccttgcgg
      241 gtgtaacctt attggcggcg actactttag ctgcatgctc tggatcaggt tcaagcacta
      301 aagctgagaa gacattctca tacatttatg agacagaccc tgataacctc aactatttga
      361 caactgctaa ggctgcgaca gcaaatatta ccagtaacgt ggttgatggt ttgctagaaa
      421 atgatcgcta cgggaacttt gtgccgtcta tggctgagga ttggtctgta tccaaggatg
      481 gattgactta cacttatact atccgtaagg atgcaaaatg gtatacttct gaaggtgaag
      541 aatacgcggt agtcaaagct caagactttg taacaggact aaaatatgct gctgataaaa
      601 aatcagatgc tctttacctt gttcaagaat caatcaaagg gttggatgcc tatgtaaaag
      661 ggggaatcac agatttctca caagtaggaa ttaaggctct ggatgatcag acagttcagt
      721 acactttgaa caaaccagaa agcttctgga attctaagac aaccatgggt gtgcttgcgc
      781 cagttaatga agagtttttg aattcaaaag gggatgattt tgccaaagct acggatccaa
      841 gtagtctctt gtataacggt ccttatttgt tgaaatccat tgtgactaaa tcttctgttg
      901 aatttgcgaa aaatccgaac tactgggata aggacaatgt gcatgttgac aaagttaaat
      961 tgtcattctg ggatggtcaa gataccagca aacctgcaga aaactttaaa gatggtagcc
     1021 ttacagcagc tcgtctctat ccaacaagtg caagtttcgc agagcttgag aagagtatga
     1081 aggacaatat tgtctatact caacaagact ctattacgta tctagttggt acaaatattg
     1141 accgtcagtc ctataaatac acatctaaga ccagcgacga acaaaaggca tcgactaaaa
     1201 aggctctctt aaacaaggat ttccgtcagg ctattgcctt tggttttgat cgtacagcct
     1261 atgcctctca gttaaatgga caaactggag caagcaaaat cttacgtaat atctttgttc
     1321 caccaacatt tgttcaagca gatggtaaaa actttggcga tatggtcaaa gagaaattgg
     1381 tcacttatgg ggatgaatgg aaggatgtta atcttgcaga ttctcaggat ggtctttaca
     1441 atccagaaaa agccaaggct gaatttgcta aagctaaatc agccttacaa gcagaaggtg
     1501 tgactttccc aattcatttg gatatgccag ttgaccagac agcaactaca aaagttcagc
     1561 gcgtccaatc tatgaaacaa tccttggaag caactttagg agctgataat gtcattattg
     1621 atatccaaca actacaaaaa gacgaagtaa acaatattac atattttgct gaaaatgctg
     1681 ctggcgaaga ctgggattta tcagataatg tcggttgggg tccagacttt gccgatccat
     1741 caacctacct tgatatcatc aaaccatctg taggagaaag tactaaaaca tatttagggt
     1801 ttgactcagg ggaagataat gtagctgcta aaaaagtagg tctatatgac tacgaaaaat
     1861 tggttactga ggctggtgat gaggctacag atgttgctaa acgctatgat aaatacgctg
     1921 cagcccaagc ttggttgaca gatagtgctt tgattattcc aactacatct cgtacagggc
     1981 gtccaatctt gtctaagatg gtaccattta caataccatt tgcattgtca ggaaataaag
     2041 gcacaagtga accagtcttg tataaatact tggaacttca agacaaggca gtcactgtag
     2101 atgaatacca aaaagctcag gaaaaatgga tgaaagaaaa agaagagtct aataaaaagg
     2161 ctcaagaaga tctcgcaaaa catgtgaaat aactgttgca aaatataaga aaggatttag
     2221 tatttctctt gaatgctgaa tcctttttta catttgtaaa gaaagattct aaatgtactg
     2281 acccccaaaa gttggagcct ctttttgtca gaatagagaa aatttttgtt aattttactt
     2341 gtttcctatt gctttctcag ctattatttg ttatattaaa agtataatta ttttttattt
     2401 atcagggtta agcattgcac tttcagagga aggagtattt tttaaaaaga aaatgtaaac
     2461 gcttgctcaa aaatgaaagg atttagaagt ttatgaataa aggattattt gaaaaacgtt
     2521 gtaaatatag tattcggaaa ttttcattag gtgttgcttc tgttatgatt ggagctgcat
     2581 tctttgggac aagtccggtt cttgcagata gcgtgcagtc tggttccacg gcgaacttac
     2641 cagctgattt agctactgct cttgcaacag caaaagagaa tgatgggcgt gattttgaag
     2701 cgcctaaggt gggagaagac caaggttctc cagaagttac agatggacct aagacagaag
     2761 aagaactatt agcacttgaa aaagaaaaac cggctgaaga aaaaccaaaa gaggataaac
     2821 ctgcagctgc taaacctgaa acacctaaga cggtaacccc tgaatggcaa acggtagaga
     2881 aaaaagaaca acagggaaca gtcactatcc gagaagaaaa aggtgtccgc tacaaccaac
     2941 tatcctcaac tgctcaaaat gataacgcag gtaaaccagc cctgtttgaa aagaagggct
     3001 tgaccgttga tgccaatgga aatgcaactg ttgatttaac cttcaaagat gattctgaaa
     3061 agggcaaatc acgctttggt gtcttcttga aatttaaaga taccaataat aatgtttttg
     3121 tcggttatga caaggatggc tggttctggg agtataaatc tccaacaact agcacttggt
     3181 atagaggtag tcgtgttgct gctcctgaaa caggatcaac aaaccgtctc tctatcactc
     3241 tcaagtcaga cggtcagcta aatgccagca ataacgatgt caatctcttt gacacagtga
     3301 ctctaccagc tgcggtcaat gaccatctta aaaatgagaa gaagatcctt ctcaaggcgg
     3361 gctcttatgg caatgaccgt acggttgtca gcgttaaaac agacaatcaa gaaggcgtaa
     3421 aagcggatga tactcctgcc cagaaagaaa caggtccggt tgttgacgat agcaaggtga
     3481 cttatgatac gatccagtct aaggtactta aggcagtaat tgaccaagcc ttccctcgtg
     3541 tcaaggaata caccttgaat ggacatactt tgccaggaca ggtgcaacag ttcaaccaag
     3601 tctttatcaa taaccaccga atcacccctg aagtcactta taagaaaatc aatgagacaa
     3661 cagcagagta cttgatgaag attcgcgatg atgctcactt aatcaatgcg gaaatgacag
     3721 tacgcttgca agttgtggac aatcaattgc actttgatgt gaccaagatt gtcaaccaca
     3781 atcaagtcac tccaggtcaa aagattgatg acgaaagaaa actactttct tctattagtt
     3841 tcctcggcaa tgctttagtc tctgtttcta gtgatcaaac tggtgctaag tttgatgggg
     3901 caaccatgtc aaacaatacg catgtcagcg gagatgatca tatcgatgta accaatccaa
     3961 tgaaggattt ggctaagggt tacatgtatg gatttgtttc tacagataag cttgctgctg
     4021 gtgtttggag taactctcaa aacagctatg gtggtggttc gaatgactgg actcgtttga
     4081 cagcttataa agaaacagtc ggaaatgcca actatgtagg aatccacagc tctgaatggc
     4141 aatgggaaaa agcttataag ggcattgttt tcccagaata cacgaaggaa cttccaagtg
     4201 ctaaggttgt tatcactgaa gatgccaatg cagacaagaa cgttgattgg caagatggtg
     4261 ccattgctta tcgtagcatt atgaacaatc ctcaaggttg ggaaaaagtt aaggatatca
     4321 cagcttaccg tatcgcgatg aactttggtt ctcaagcaca aaatccattc cttatgacct
     4381 tggatggtat caagaaaatc aatctccata cagatggtct tgggcaaggt gttctcctta
     4441 aaggatatgg tagcgaaggc catgactctg gtcacttgaa ctatgctgat attggtaagc
     4501 gtatcggtgg tgtcgaagac ttcaagaccc taattgagaa ggctaagaaa tatggagctc
     4561 atctaggtat ccacgttaac gcttcagaaa cttatcctga gtctaaatac ttcaatgaaa
     4621 aaattctccg taagaatcca gatggaagtt atagctatgg ttggaactgg ctagatcaag
     4681 gtatcaacat tgatgctgcc tatgacctag ctcatggtcg tttggcacgt tgggaagatt
     4741 tgaagaaaaa acttggtgac ggtctcgact ttatctatgt ggacgtttgg ggtaatggtc
     4801 aatcaggtga taacggtgcc tgggctaccc acgttcttgc taaagaaatt aacaaacaag
     4861 gctggcgctt tgcgatcgag tggggccatg gtggtgagta cgactctacc ttccatcact
     4921 gggcagctga cttgacctac ggtggctaca ccaataaagg tatcaacagt gccatcaccc
     4981 gctttatccg taaccaccaa aaagatgctt gggttgggga ctacagaagt tatggtggtg
     5041 cagccaacta tccactgcta ggtggctaca gcatgaaaga ctttgaaggc tggcaaggaa
     5101 gaagtgacta caatggctac gtgactaacc tctttgctca tgacgtcatg actaagtact
     5161 tccaacactt cactgtaagt aaatgggaaa atggtacacc ggtgactatg accgataacg
     5221 gtagcaccta taaatggact ccagaaatgc gagtggaatt ggtagatact gacaataata
     5281 aagtagttgt aactcgtaag tcaaatgatg tcaatagccc acaatatcgc gaacgtacag
     5341 taacgctcaa cggacgtgtg atccaagatg gttcagctta cttgactcct tggaactggg
     5401 atgcaaatgg taagaaactt tctactgata aggaaaagat gtactacttc aatacgcagg
     5461 ccggtgcaac aacttggacc cttccaagcg attgggcaaa gagcaaggtt tacctttaca
     5521 agctaactga ccaaggtaag acagaagagc aagaactaac tgtaaaagat ggtaaaatta
     5581 ccctagatct tctagcaaat caaccatacg ttctctatcg ttcaaaacaa accaatcctg
     5641 aaatgtcatg gagtgaaggc atgcacatct atgaccaagg atttaacagt ggtaccttga
     5701 aacattggac catttcaggc gatgcttcta aggcagaaat tgtcaagtct caaggggcaa
     5761 acgatatgct tcgtattcaa ggaaacaaag aaaaagttag tctcactcag aaattaactg
     5821 gcttgaaacc aaataccaag tatgccgttt atgtcggtgt cgataaccgt agtaatgcca
     5881 aggcaagtat cactgtgaat actggtgaaa aagaagtgac tacttatacc aataagtctc
     5941 tcgccctcaa ctatgtaaaa gcctacgccc acaatacacg tcgtgacaat gctacagttg
     6001 acgatacaag ttacttccaa aacatgtacg ccttctttac aactggatcg gacgtctcaa
     6061 atgttactct gacattgagt cgtgaagctg gtgatcaagc aacttacttt gatgaaattc
     6121 gtacctttga aaacaattca agcatgtacg gagacaagca tgatacaggt aaaggcacct
     6181 tcaagcaaga ctttgaaaat gttgctcagg gtatcttccc atttgtagtg ggtggtgttg
     6241 aaggtgttga agataaccgc actcacttgt ctgaaaaaca cgatccatat acacaacgtg
     6301 gttggaatgg taagaaagtc gatgatgtta tcgaaggaaa ttggtcactc aagacaaatg
     6361 gactagtgag ccgtcgtaac ttggtttacc aaactattcc gcaaaacttc cgttttgaag
     6421 caggtaagac ctaccgtgta acctttgaat acgaagcagg ttcagacaat acctatgctt
     6481 ttgtagtcgg taagggagaa ttccagtcag gtcgtcgtgg cactcaagca agcaacttgg
     6541 aaatgcatga attgccaaat acttggacag attctaagaa agccaagaag gcaacattcc
     6601 ttgtgacagg tgcagaaaca ggggatactt gggtaggtat ctactcaact ggaaatgcaa
     6661 gtaatactcg tggtgattct ggtggaaatg ccaacttccg tggttataac gacttcatga
     6721 tggataatct tcaaatcgaa gaaattaccc taacaggtaa gatgttgaca gaaaatgctc
     6781 tgaagaacta cttgccaacg gttgccatga ctaactacac caaagagtct atggatgctt
     6841 tgaaagaggc ggtctttaac ctcagtcagg ccgatgatga tatcagtgtg gaagaagcgc
     6901 gtgcagagat tgccaagatt gaagccttga agaatgcttt ggttcagaag aaaacggctt
     6961 tggtagcaga agactttgca agtcttacag ctcctgctca ggctcaagaa ggtcttgcaa
     7021 atgcctttga tggaaactta tctagtttat ggcatacatc atggggcgga ggagatgtag
     7081 gcaagcctgc aaccatggtc ttgaaagaac caactgaaat cactggactt cgttatgttc
     7141 cacgtggatc aggttcaaat ggtaacttgc gtgatgtgaa acttgttgtg acagatgagt
     7201 ctggcaagga gcataccttt actgcaactg attggccaga taacaataag ccaaaagaca
     7261 ttgattttgg taagacaatt aaggctaaga aaattgtcct tacaggtact aagacttacg
     7321 gagatggtgg cgataaatac caatctgcag cggaactcat ctttactcgt ccacaggtag
     7381 cagaaacacc tcttgacttg tcaggctatg aagcggcttt ggctaaggct cagaaattaa
     7441 cagacaaaga caatcaagag gaagtagcta gcgttcaggc aagcatgaaa tatgcgacgg
     7501 ataaccatct cttgacggaa agaatggtgg aatactttgc agattatctc aaccaattaa
     7561 aagattctgc tacgaaacca gatgcttcaa ctgtagagaa acctgagttt aaacttagct
     7621 cacttgcttc agatgaagat aaggcaccgg caccggaact aaaacaagaa gtgacgaagc
     7681 gagaaacagc tgaacaaagc ttgccagcaa caggtgagag tcaatctgac acagccctct
     7741 tcctagcagg agttagccta gccctatctg ctctctttgt agcaaaaaca aagaaagact
     7801 agtatttagt aaaacctctt aacaagatta cgaaatccgt ttcctatctt tcccaatgag
     7861 gtttaaagta cagaaaaagc ctgagaagac atcttctcag gcttttgtta agcatataaa
     7921 tgcaatagtg ctatgaaaaa atcacccaga aaaatctggg tgataaatgt tatggttgtg
     7981 ctggttgagg attctgattt tggttctggt tttgttgacc aggcgttgta tttggttgct
     8041 ggttattggt attattgttt gcactattat tcgtgcttgg agtagttgag cttgactgtg
     8101 aagttgaact ttctgatgtc gagctcgaac tttctggtgt tggggcctgt tgcggagcag
     8161 gagcagtcca tgcagaacga gcaccttttt taaagacgaa ctcaccactt cgatagagac
     8221 cttctggcat agtccagtcg ccagggttgt tatcctcaga cagataagtc atcattgaac
     8281 ggtaaacctt agctgcaaca tagaagccat caccaacgat aggagtcagg cggtttgagt
     8341 aacctgtcca taccgccatt gaatatttgc gagtataacc aacaaacatt tcgtctggag
     8401 ctacataacc agtatttttg atgtagtttt caatttcatc atctgtatag tttgatgtac
     8461 cagtcttacc agcttgaggt agccaaggga gataagcacc acgacccgtt ccgtatgcca
     8521 agacagtctt catcatttct gtcatcatat aggcggtcgt ctctttcatg gcacgagtac
     8581 caggatcagc gtattctttt gagctaccat cgctaaagac gattttgttg atatacattg
     8641 gtttatggta gataccaccg ttagcaaaag cagcgtaagc agcagccatt ttctcactac
     8701 ttgctccgta ctttttgttt gactcagtcg tattacttga aatggcgttt gcataatgca
     8761 tgcttggata gtcgatacca agaccattaa gaaaggtttt agctctatct agaccgacct
     8821 tattcaaagt ctcaacggct gtgacattac gtgactgttg aagtgcatac tggattgtaa
     8881 tatttccaaa gtagacttta tcccagttgt agaggggagt atcagtacct ggatagttat
     8941 aaggaacatc atgtacaatc gaagcagttg agtcgtagat gtcatactct aaggcaggag
     9001 catagtctgt gattggcttc atggcagaac cccagtcacg gttggtttca acagcttggt
     9061 tgattccgaa ggaaacattg cttgattggt gacgagaacc taactgagca ataactttcc
     9121 cgttagaaac atcaacaatg gtagaagcga cttgcaattc atcgtctgga taggcaacgt
     9181 attcgtctgt attgtaaata tcccacagat gtttttgagc ttcttggtct acatttgtgt
     9241 agacatccat cccagttgtg agcaggttat agcctgtttc ttcttcaact tgattgatga
     9301 cttccttgag gtaattatcc atgtaagcag ggtaattact tgctgatttg agactttgta
     9361 gtccatcagt aattggtgta ttgactgctt tctcatactg ttcagcagag atgtagcctt
     9421 gatttttcat ttcagataag accaagtttc ggcggtcttg ggctgcttct ggatgtgaat
     9481 aggggtcata ttggtttggt gcctgaggca ttccagccag caaggctaac tgaggtaaac
     9541 ttaaattatt gaggtcttta ccatagtagt tttgagctgc tgtctgcatt ccatagttcc
     9601 cattagacat gtagacctta tttatatagt aggtcaagat ttcttgcttg gttgcttttt
     9661 gttctaactg aatcgctaac caagcttcct gagccttacg agaaatagtc tggtcggaag
     9721 tcgaagttga aaagtaagtc aacttaatca actgttgagt gagagttgat ccaccttgga
     9781 gggaattgct ttgcagattg cgcaagaaag ctcccaggat acggatggta tcaatccccc
     9841 tgtggtcgaa gaagcgatgg tcttcgatag aaacgattgc cttaaccaaa tctgtgggaa
     9901 tatcattagc ttgggcattg acgcggcgtt cagaacccaa gtcagcaatg agttgatttt
     9961 tattgtcgta gattttacta gaagttgttg caactagttt actctcggat aggctaggag
    10021 ccttgctaac atagtagaaa aaaactcctc cgcctaagac aatggctgcg ataaccaagc
    10081 ttaagaagct aatgctcaga tacttgatta ggcgcagaat cgttggtttg ttcatcttgt
    10141 tttaccacct aataaatgtt ctttgataac attgagataa ggaatttgag ggaaggcacc
    10201 agccttgatt tcatatccat attctcgaat atattcaagt ggcattgatt tttgtccctt
    10261 atcttgatga tagaagcgaa tcaaatcgaa tgccggcaat aagtaggttt cttgctgaga
    10321 agaaaagtga agaaggacaa agcagattcc ttgttgggca aggacttgtt ccatatgctg
    10381 aatctgatgt ggatgaaaat ttttcatcgg aatcgcacgt ttttgttttg tttccttgac
    10441 ttcaaagtcg atgtaatatc cattataaac gccagaatag tccgtcgttg aagcttgtcg
    10501 aaaataggct tcaacaatct tggcacgact tcgttgtgga tagtccactt gtacgatttg
    10561 aataggagtt ggtttcttat gtataacagc caagccttga gacaaatagt agtcgttggt
    10621 agcattgatc atcttttcaa aagacattcc tcgatttgcg aaatttttgg gttgagaaag
    10681 agatgtttgt cttttttgtg atgaaacttt atgtggatag ttgaccataa ttctccttat
    10741 tggtacaata acatcacttt attataccat aatttgcaca gaaagggtta aaaatggcta
    10801 cagctttggt tttaggctat tctgcttttg atttgggttt attttctgat aaggatcctc
    10861 gtcttaaatt gattaaaaaa gcgatacgta aagatttaga ggctatggca gcagatgggg
    10921 tgtcttggct tgtgtttaca gggagtttgg ggtttgaata ttgggtttta gaggttgctc
    10981 aggagatgaa aaccgaatac ggtttccagt tggccaccat ttttgctttt gaaacccatg
    11041 gggaaaattg gaatgaaggc aatcagatga aactgagtcg ctttaagcag gtagactttg
    11101 tcaaatatgc ctatcctcgc tatgaacaca aggggcagtt aagagattac cagcagtttt
    11161 tgctggaaaa cacgactagt tcctatcttt tttatgatga agaaaatgaa acaaaactag
    11221 cttattttta ccaaaagatg aaaaatcaag aggactattt tataaagaga ttaacatttg
    11281 atcaattaaa tgaacttgct gaaaattttt ccgaaaattg aagctttgac cttgattttt
    11341 gtttgccttt ttttatataa taatactagc aagcgagaat ggagagagac atggcaagta
    11401 ttattttttc agcgaaagat atttttgaac aagagtttgg acgtgaagtc cgtggctata
    11461 ataaagtaga agttgacgag tttttagacg atgtcatcaa ggactatgaa acctatgctg
    11521 ccttggtcaa gtcacttcgt caggaaattg cggatttgaa ggaagaatta actcgtaaac
    11581 cgaaaccttc accagttcaa gcagaacccc ttgaagcggc aattacaagt tctatgacga
    11641 attttgatat tttgaaacgc ctgaatagat tggaaaaaga agtttttggt aaacaaattt
    11701 tagataactc agatttttaa gtagttattt gagatgtgca atttttggat aatcgcgtga
    11761 ggagaattgc ttctcatgag gaaagtccat gctagcacag gctgtgatgc ctgtagtgtt
    11821 tgtgctaggc gaaaccataa gcctagggac gagaaatcgt tacggcagtt gaaatggcta
    11881 agtccttgga taggccagag taggcttgaa agtgccacag tgacggagtc tttctggaaa
    11941 cagagagagt ggaacgcggt aaacccctca agctagcaac ccaaattttg gtcggggcat
    12001 ggagtacgcg gaaacgaacg tagtattctg actgctatca gctagagctg ttagtggtag
    12061 acagatgatt atcgaaggaa gtggtcctag tcacttctgg aacaaaacat ggcttataga
    12121 aaattgcata taggttgggg ctgagaaatt ttctcaacct cattttttaa agtggacata
    12181 tagaaaggtc ttgcaagact gtaacatgaa aaaagaattt aatttaattg caactgtggc
    12241 agcagggctt gaggctgtcg ttggtcgtga agtgcgagag ttgggctacg attgtcagat
    12301 tgaaaatgga cgtgttcgtt ttcaaggaga cgtgagagct attatcgaaa ccaacctttg
    12361 gctgcgggca gcagatcgta tcaaaattat cgtaggaacg ttccctgcta agacttttga
    12421 agagctattt cagggagttt tcgctttaga ttgggaaaat tatttaccac ttggagctcg
    12481 gttcccgatt tcaaaagcta aatgtgttaa gtccaaactt cacaatgagc ccagtgttca
    12541 ggctatttct aagaaagctg ttgtcaagaa attgcagaaa cactatgctc gcccagaagg
    12601 ggttcctctg atggagaatg gcccagagtt taagattgag gtctctattc tcaaagatgt
    12661 ggcaactgtc atgattgata cgaccgggtc tagcctcttt aaacgtggtt atcgtaccga
    12721 aaaaggtggc gctcctatca aggaaaatat ggcagcagcc attttacaac tttctaactg
    12781 gtatccagac aagcctttga ttgatccgac ctgtggttcg gggactttct gtattgaggc
    12841 agttatgatt gctagaaaga tggcgccagg tcttcgtcgc tcttttgcat ttgaggaatg
    12901 gaactggatc agcgatcgct tgattcaaga agtgcgcaca gaagcggcta aaaaagtaga
    12961 ccgtgagctt gagctggata tcatgggctg tgatattgat gctcgcatgg tggaaattgc
    13021 taaggccaat gctcaggtag ctggtgttgc aggagacatt acttttaagc agatgcgcgt
    13081 gcaggattta cgttccgata aaatcaatgg agtaatcatt tccaatccgc cttatggtga
    13141 acgtttgtca gatgatgcag gggtgaccaa gctctatgct gagatggggc aagtatttgc
    13201 accgctgaaa acttggagca aatttatcct gactagtgat gaagcttttg aaagcaagta
    13261 tggtagccaa gcagataaga agcgtaagtt atacaacgga accttgaaag tggatctata
    13321 tcaatatttt ggtcagcgtg tcaaacggca agaggtaaaa tagaaaggat actcatgagt
    13381 aaaaaaagac gaaatcgtca aaaaaaagaa ggtcaagaac cgcaatttga ttttgatgaa
    13441 gcaaaagagc taacagttgg tcaagctatt cgtaaaaatg aagaagtgga agcaggagtc
    13501 ttgcctgagg attccatttt ggacaagtat gttaagcaac acagagatga aattgaggcg
    13561 gataagtttg cgactcgtca atacaaaaaa gaggagttcg ttgaaactca gagtctggat
    13621 gatttaattc aagagatgcg tgaggctgta gagaagtcag aagcttcttc ggaggaagtt
    13681 ccatcttctg aagacatctt actacccttg cctctggacg atgaggagca aggcttggat
    13741 cctctattgc tagatgatga aaatccaaca gaaatgactg aagaagtgga agaggagcaa
    13801 aacctttctc gtctggatca agaggactca gaaaagaaaa gtaaaaaagg ctttattttg
    13861 accgttttgg cgcttgtatc agtaattatt tgtgtcagtg cttattatgt ctaccgtcaa
    13921 gtggctcgtt cgaccaagga aattgaaact tctcaatcaa ctacagccaa tcaatcggat
    13981 gtggatgatt ttaatacact ttatgacgcc ttttacacag atagcaataa aacggctttg
    14041 aaaaatagcc agtttgataa actgaatcaa ctcaagactt tacttgataa gctggaaggt
    14101 agtcgtgaac atacgcttgc caaatctaaa tatgatagtc tagcaacgca aatcaaggct
    14161 attcaagatg tcaatgcaca atttgaaaaa tcagctattg tggatggtgt gttggatacc
    14221 aatgccaaag ccaaatcgga tgctaaattt acagatatta aaactggaaa tacggagctt
    14281 gataaagtgc tagataaggc tatcagtctt ggtaagagcc agcaaacaag tacttctagc
    14341 tcaagtttaa gtcaaactag cagctcaagt tcaagtcaag caagttcaaa tacgactagt
    14401 gagccaaaac caagtagttc aaatgagact agaagtagtc gcagtgaagt caatatgggt
    14461 ctctcgagtg caggggttgc tgttcaaaga agtgccagtc gtgttgccta taatcagtct
    14521 gctattgatg atagtaataa ctctgcctgg gattttgcgg atggtgtttt ggaacaaatt
    14581 ctagcaactt cacgttcacg tggctatatc actggagacc aatatatcct tgaacgtgtc
    14641 aatatcgtta acggcaatgg ttattacaac ctctataagc cagatggaac ctatctcttt
    14701 acccttaact gtaagacagg ctactttgtc ggaaatggcg ctggtcatgc ggatgacttg
    14761 gactactaag cagtcgttac aaaattcttt cttttcaaaa gtaaaaatga taaaataaaa
    14821 caaatgaaac aagaggagtg tcaaatgaca aaagctaact ttggtgtcgt aggtatggcc
    14881 gtaatgggtc gtaaccttgc ccttaatatt gaatcacgtg gttacacagt tgctatctac
    14941 aaccgtagta aagaaaaaac ggaagatgtg attgcttgcc atcctgaaaa gaattttgta
    15001 ccaagctatg acgttgaaag ttttgtaaac tcaatcgaaa aacctcgtcg tatcatgctg
    15061 atggttcaag ctggacctgg tacagatgct actatccaag cccttcttcc acaccttgac
    15121 aaaggtgata tcttgattga cggaggaaac actttctaca aagataccat ccgtcgtaat
    15181 gaagaattgg caaactcagg tatcaacttt atcggtactg gagtttctgg tggtgaaaaa
    15241 ggtgcccttg aaggtccttc tatcatgcct ggtggacaaa aagaggccta cgaattggtt
    15301 tcggatgttc ttgaagaaat ctcagctaaa gcaccagaag atggcaagcc atgtgtgact
    15361 tatatcggtc ctgatggagc tggtcactat gtgaaaatgg ttcacaacgg tatcgagtac
    15421 ggtgatatgc aattgatcgc agaaagttat gacttgatgc aacacttgct aggtctttct
    15481 gcagaggata tggctgaaat ctttactgag tggaacaagg gtgaattgga tagctacttg
    15541 atcgaaatca cagctgatat cttgagccgt aaagacgatg aaggccaaga tggaccaatc
    15601 gtagactaca tccttgatgc tgcaggtaac aagggaactg gtaaatggac tagccaatca
    15661 tctcttgacc ttggtgtacc attgtcactg attactgagt cagtgtttgc acgctacatt
    15721 tcaacttaca aagaagaacg tgtatatgct agcaaggtgc ttccaaaacc agctgccttc
    15781 aactttgaag gagacaaggc tgaattgatt gaaaagatcc gtcaagccct ttacttctca
    15841 aaaatcattt catacgcaca aggatttgct caattgcgtg tagcctctaa agaaaacaac
    15901 tggaacttgc catttgcaga tatcgcatct atctggcgtg atggctgtat catccgttct
    15961 cgtttcttgc aaaagattac agatgcttac aaccgcgatg cagatcttgc caaccttctt
    16021 ttggacgagt acttcttgga tgttactgct aagtaccaac aagcagtacg tgatatcgta
    16081 gctcttgcgg ttcaagcagg tgtgccagtg ccaactttct cagcagctat tacttacttt
    16141 gatagctacc gttcagctga ccttccagct aacttgatcc aagcacaacg tgactacttt
    16201 ggtgctcaca cttaccaacg taaagacaaa gaaggaacct tccactactc ttggtatgac
    16261 gaaaaataag taggtcagcc atggggaaac ggattttatt acttgagaaa gaacgaaatc
    16321 tagctcattt tttaagtttg gaactccaga aagagcagta tcgggttgat ctggtagagg
    16381 aggggcaaaa agccctctcc atggctcttc agacagacta tgatttgatt ttattgaacg
    16441 ttaatctggg agatatgatg gctcaggatt ttgcagaaaa attgagccga actaaacctg
    16501 cctcagtcat catgatttta gatcattggg aagacttgca agaagagctg gaagttgttc
    16561 agcgttttgc agtttcatac atctataagc cagtccttat cgaaaatctg gtagcgcgta
    16621 tttcggcgat cttccgaggt cgggacttca ttgatcaaca ctgcagtctg atgaaagttc
    16681 caaggaccta ccgcaatctt aggatagatg ttgaacatca cacggtttat cgtggtgaag
    16741 agatgattgc tctgacacgc cgtgagtatg accttttggc gacacttatg ggaagcaaga
    16801 aagtattgac tcgtgagcaa ttgttggaaa gtgtttggaa gtatgaaagt gcgaccgaga
    16861 caaatatcgt agatgtctat atccgctatc tacggagcaa gcttgatgtt aaaggacaaa
    16921 aaagctacat taaaactgtg cgtggtgttg gatataccat gcaagaatag aaaagcagtt
    16981 gcagttgtgt aactgctttt ttgaggagtt tttatatatt gacatgcagt cgggtctttg
    17041 ctacaatcgt tttaggagga tatatagatg aaacttttga aaaaaatgat gcaagttcta
    17101 ctagcagtct ttttctttgg tttgctagct acaaatacgg tatttgcgaa taccacaggt
    17161 ggccgatttg ttgataagga taatagaaaa tattatgtaa aagatgatca taaagcaatc
    17221 tattggcata aaatagacgg taaaacttac tattttggtg atattggaga gatggttgtc
    17281 ggttggcaat acttagaaat tcctggaaca ggttatcgtg ataatttatt cgataaccaa
    17341 ccagttaatg aaattggcct tcaggagaag tggtactatt ttggacaaga tggtgctttg
    17401 ctagaacaaa cagataaaca agtactagag gcaaaaacgt ctgaaaatac aggaaaagta
    17461 tacggtgaac aatatcctct atctgctgaa aagagaactt attattttga taataattat
    17521 gctgtaaaga caggctggat ttatgaagac ggcaattggt attatttaaa taagctagga
    17581 aattttggcg atgattctta caatccacta ccaattggtg aagttgctaa gggttggact
    17641 caagattttc atgttactat tgacattgat agaagcaaac ctgctccatg gtactaccta
    17701 gatgcttcag gtaagatgct tacagattgg caaaaagtaa acggaaaatg gtattatttt
    17761 ggctcctctg gttctatggc aacaggttgg aaatatgtac gaggcaaatg gtattactta
    17821 gataataaaa atggtgatat gaaaacagga tggcaatacc ttggtaacaa gtggtactac
    17881 ctccgttcat caggagcaat ggcaactggt tggtatcaag atggttcaac ttggtactac
    17941 ctaaatgcaa gtaatggagg tatgaagaca ggttggttcc aggtcaatgg caaatggtat
    18001 tatgcctatg gttcaggtgc tttggcagtg aatacgaccg tagacggcta ttctgtcaat
    18061 tataatggcg actgggttcg gtaatgacag ggtgcttata attatattag aacactcgct
    18121 taatcaaaga agtgactttg ttgtacttga cctgacgatt ggggagatta aaaagcatgt
    18181 gaaatctaca catgcttttt tgttagttat gagtgttgaa cttgactaac cattgacaaa
    18241 agataagagc aatcaaaata agatacaaaa atatcaatgc taactgcacc gaaacgcccg
    18301 atgctatgga tagggctaat agggagagca gggacggggc gagaatgtcc actgtgctca
    18361 gagcagagcc caccgtcccc atcttgtcac tggggatatg ggcaaagaca gctgcttgta
    18421 ggcgagggct gagaatgccg ataatcgtag aactgatgaa actggtcatg aaaattacga
    18481 tataggcttg acaaaggata ctagttatta gagccaatag ggaaatctca cagaaaacca
    18541 agaggtgcgt gaaacggata tttttaaata gattactgct ggtcatattg ccaaggatag
    18601 ctccacaaga aatgatgatg gagagcatgg cgaggagttg cccaatgctc agttgtgcta
    18661 gtgtctgttg gatgaagcgg agggcaatca gccgagggga cacatccaag atggcaatca
    18721 tactggtcgt cagaaacagt agtatgacgg tatcttttaa cctcaggagc acagccagcg
    18781 actgtttaag atgttgaaaa tatttcttaa aactcagtgc tgtatgtgac atttcaatcc
    18841 ttttttcaac ctcatacaag gtatgtcgaa taacatacag gcccaaaaag gcaatgacaa
    18901 aagtcagagt gttgataagg gaaatagttt gaatactcat aacattgata aggaatgcgc
    18961 cagccagatt ggcgacaatg cggacaaccc tcatcagaga ctgcctgaac cccatggcat
    19021 catgcaggtc gtccttaatt acactgatgt agagggcgtt catcatgtag gcagacaggt
    19081 agctcaaggt gtcagagatg aggttgatga tgacaatcat aatgactgaa actaatgaaa
    19141 agggcgacaa gacgagaaat actgtcaaaa tagcgtagag caggattttg attttggcaa
    19201 tccagagtgc gtgtttgact ctattttctt gaaaatctcc tagaatcccg agaaagagtt
    19261 gcgatagtag gggaaccatc tctgaaatgg aaatcagtaa aattgctagc ggagcgaaat
    19321 tgtactgagc cacataattg ataatggcga ggtagaaaat actgtcgccg aatgatgaaa
    19381 aacagctgtt tagcagcaac tgtctatata tgttgtttgt ccaaaataat ttcatacttt
    19441 tctccttatg tctgcacatc gaatgggaat ttattattta atagacacca atataccccc
    19501 atgcttccta ttccgaagtc aaacaaatct gctttaccac tatctccttg aaacatcagt
    19561 aaaccgtgat catctcgttc agaacatgct aaaattacct gttggatttt tttcatcagt
    19621 aatttgtttt gattgtagac agtggtttgt agtaagctag ttattccgtg gcaatatcct
    19681 gtcatcattt ttagatgatg gttaaaaaca aattcttgat atttactaat aatatctttg
    19741 tttccgtcac aatcgtacat acaaagatat aagattattc cggaaattcc ttcgcaccaa
    19801 gaaagttgta aatttcctaa attttcagta ttagaagtaa ctttttctaa taattttttt
    19861 aattcagtat ggaatgtatg gagatcatta tagaacatag aaggttctaa gactttagaa
    19921 taggcaaata aagcctatgc aattccacaa tatccatgag caaagctata gtctaaaatg
    19981 ctttctttgg ctatatctct ttgtaggaaa taatgctaaa tttcccctat actattgtgg
    20041 atgaaaattt taagagtatt gtcattggta aattgatagt attttattaa tgataatagg
    20101 acaccagatt ttcccaatgc aaagtctatt tcctctagag tatcataatt ctctattaat
    20161 ttcttagcgg agacgtttgc taattgtaga tagtattgat ttttactgat agaaaataaa
    20221 tgtaggagta actatagata gcctgcatca ccaaatagca gtgagtattg atattcatat
    20281 gtgtgtgtcg ttctaattgt cactgccttc ttgatgatag agagtatttt agaatccagt
    20341 tctggggggt acaatttatt tagatgaaac agtagtccag ctattccgtg ttggaatgat
    20401 aaattactta cgaaatttcc aaattctgtt gaagaagtga tccttccttc agaatttaca
    20461 gttttatcta gtaaaaatcg tagaacacta ttacattctg ttttgtaatc atatttatgt
    20521 tcctctattt ttttaatttt gactaattat ttgtttgact taaatttagt agacattgta
    20581 accaaaatag ttgctcatct gtgagctgac cttgagtatg tgatagttct agtaattgaa
    20641 taggatcctt taggtagatt caatcaatct ataacacaac aacataataa tacttttagg
    20701 ataacttgtc aaatattata gtaaaatgaa ataa
//
LOCUS       DAVMEI010000019        20134 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVMEI010000019 DAVMEI010000000
VERSION     DAVMEI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.019"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            32..1963
                     /locus_tag="VYQ24_000568"
     CDS             32..1963
                     /locus_tag="VYQ24_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1967745.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYISPEEVETDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQTVADDNEGDENDDADY"
     gene            1950..3266
                     /locus_tag="VYQ24_000569"
     CDS             1950..3266
                     /locus_tag="VYQ24_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1967746.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            3441..4427
                     /locus_tag="VYQ24_000570"
     CDS             3441..4427
                     /locus_tag="VYQ24_000570"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1967747.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDETGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4491..4562
                     /locus_tag="VYQ24_000571"
     tRNA            4491..4562
                     /locus_tag="VYQ24_000571"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4524..4526,aa:Arg,seq:ccg)
     gene            4745..5443
                     /locus_tag="VYQ24_000572"
     CDS             4745..5443
                     /locus_tag="VYQ24_000572"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1967748.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5434..8973
                     /gene="smc"
                     /locus_tag="VYQ24_000573"
     CDS             5434..8973
                     /gene="smc"
                     /locus_tag="VYQ24_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1967749.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALDIALGASSQHIIVEDENAATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEVALKTLQDEMARLTESLEAIKSQGEQARIQEQGLFLAYQQTSQQV
                     EELETLWKLQEEEKDRLSEGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVTDIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTDLLSQQADEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8970..9764
                     /locus_tag="VYQ24_000574"
     CDS             8970..9764
                     /locus_tag="VYQ24_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1967750.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLVSYKEKGIYFAVAS
                     GRGFLSLGKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9764..10582
                     /locus_tag="VYQ24_000575"
     CDS             9764..10582
                     /locus_tag="VYQ24_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1967751.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGINQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10586..11875
                     /gene="ftsY"
                     /locus_tag="VYQ24_000576"
     CDS             10586..11875
                     /gene="ftsY"
                     /locus_tag="VYQ24_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1967752.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVDEVP
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDSEPVVGVSQEEVEEFPHSEELPEEEKLDHE
                     GTIEENNSEVLEAERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVLEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11921..13408)
                     /locus_tag="VYQ24_000577"
     CDS             complement(11921..13408)
                     /locus_tag="VYQ24_000577"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1967753.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTAATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(13557..14297)
                     /locus_tag="VYQ24_000578"
     CDS             complement(13557..14297)
                     /locus_tag="VYQ24_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1967754.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14297..16462)
                     /locus_tag="VYQ24_000579"
     CDS             complement(14297..16462)
                     /locus_tag="VYQ24_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1967755.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            16632..17585
                     /locus_tag="VYQ24_000580"
     CDS             16632..17585
                     /locus_tag="VYQ24_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1967756.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            17662..19650
                     /gene="uvrB"
                     /locus_tag="VYQ24_000581"
     CDS             17662..19650
                     /gene="uvrB"
                     /locus_tag="VYQ24_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1967757.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVQALD"
     gene            19660..>20134
                     /locus_tag="VYQ24_000582"
     CDS             19660..>20134
                     /locus_tag="VYQ24_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967758.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQG"
BASE COUNT         6204 a         3595 c         4492 g         5843 t
ORIGIN      
        1 ttaaacatca ttagtaaaag gagttagatt gatgaataaa agaaaagtta gtttagaaga
       61 tttttataaa tggtatagtc taaataaaga agagttatta aataaggcaa ctgttggtga
      121 aaagtttaat gataaattaa aagaagagtt tctccaggaa tggcctttgg ataggatttt
      181 aacaatgtca atcgatgaat atgtaatagg aaagggacag caaaataagt ctttatgcta
      241 cgctcttgag aagggaaaat acaaaaatct atttcttgga atttctggtg gctcagcttc
      301 aaaatttggt atttattgga ataaaaaaac aaacaaatat aaagatcaag ctaataatga
      361 gatttcagag ttggatcagc gattttcaaa attaaaatca gatttgtatg aaattatcaa
      421 agaaggtatt cgttttaact ttgaaaatcc tatttttgat atgaaaagat caacaaatga
      481 atttattggt cgttctgcta tggtgacaaa attactttgt atctatactg agggagatcc
      541 tttctttggt gtaaatatta atagtcagaa agaattttgg aaccactttg tttctcagac
      601 aaatcaaggt ggaccttatc tgcaaaatca taaaataatt gaactagtgt ccaaaactta
      661 tcctgagttg gagccatcga aattaggaac tatgcttttt gagtattcta agctttttat
      721 ggaaaataag gaagacaata gtacaatgga ttcatcaaac aattttagtc atcaattaac
      781 tcaatctcta ttaaagtctc caaaccttat ccttcgtggt gctcctggca caggaaagac
      841 ttatcttgct aaagaaattg ccaaagaatt aacggatggc aacgaagacc aaatcggatt
      901 tgtacaattt cacccatcct atgattatac ggattttgta gaaggtttaa gaccagtatc
      961 aaatggggat ggagctattg agtttaggct acaggacggt atttttaaag atttttgtca
     1021 gaaagcaaaa gaaacccaat tgattggagg acaagataat tttgatgagg cttgggattc
     1081 ttacttagaa tatataaatg ttgctgaaga aaaagaatat ataacaaaaa catcttactt
     1141 atctgttaat agtagacaaa atttgtcagt aaattatgat agtggtgttc caggatggtc
     1201 actacctagc aaatatgttt acgagttgta taaagataaa aattataata agcaagaata
     1261 ctacaaaagt ggtggaaaaa ctgtcctaga aacattgaga aagagatttg gtttgaaaga
     1321 ctatatttct ccagaagagg ttgaaacaga caagaaattt gttttcatca tcgatgagat
     1381 caatcgtggg gagatttcta agatttttgg cgaactcttt ttttctatcg accctggcta
     1441 tcgtggtgaa aaaggaagtg tttctaccca atatgcaaat ctacacgaaa ctgatgaaaa
     1501 gttctatatc cccgaaaatg tttacatcat cggaactatg aatgatattg atcgttcagt
     1561 ggataccttt gattttgcta tgcgtcgtcg ttttcgtttt gttgaagtta ctgccgaggg
     1621 tcaagttggc atgttggata aagagttgaa tatccatgca gaagaagcaa aaattcgtct
     1681 aagaaacttg aacgctgcta tcgaaaatgt tcaggaatta aacagtcatt atcatattgg
     1741 accaagttat tttcttaagt tgaaggatgt agattttgac tatgaattac tctggtctga
     1801 ttatattaag cctctcctag aagactactt gcgaggttct tatgatgagg ttgaaacttt
     1861 ggaaactttg aaaaaagcat ttgaactgac aaataatgag caaaaagatc agacagtagc
     1921 tgatgacaat gaaggtgatg aaaacgatga tgcggattac tgataatcaa cacaagatta
     1981 ttaaagaaaa atttgttgaa gaatatccta aactaagcaa tcttctttta gacagaacct
     2041 tggaaagtct atcccaagat gaacgtattt tcatttttcc aaatgatttg actcatactc
     2101 ctgatttgga taaggaccaa aagatttttg aaacagtcaa tcagaaaatc aagacaggga
     2161 acgtgattgg ttttcttgga tatggtcagg aaagattaac gatttcctca cgattttctg
     2221 atgagagtaa tgaccatttt ttgcattatc tcttaaacaa ggttcttcat atcaatctca
     2281 ctagtttaga tgttgctttg tctcgtgaag agaggcttta tcaacttttg atgtatctct
     2341 tccccaagta tctacaagct gctattcgaa aaggtcttta taaggaatat catcgatttt
     2401 ctcataacga cagtcatgtt aagggagtga ttgatgtaag aagccatctc aagaaaaatc
     2461 ttcctttcac gggaaatatt gcctacgcaa cgagagagtt cacctatgat aatcccctca
     2521 tgcagttggt ccgtcacact attgaataca ttaagaatca gaaaagcatt ggtcaagggg
     2581 tactagataa tctctcaact agtcgtgaaa acgtatctga aatcgtgcgt gtaacgccct
     2641 cttataaact agctgatcgt gctaagatta ttcggggaaa tcaatctaaa cctatacgtc
     2701 atgcatactt tcacgagtac agaaacttac aagaactttg tctgatgatc ctaaaccaag
     2761 aaaagcacgg tttagggtat caagatcaaa aaatctatgg tattctcttt gatgttgcct
     2821 ggctttggga agagtatgtt tacaccttgt tgccaaaagg ttttgtacat cccagaaata
     2881 aggataagac ggatggaatt tcagtatttt ctgttgggaa acgaaaagta tatccagatt
     2941 tttatgacag agaacgaaag attgttctag atgcaaaata taaaaaactg gaattgactg
     3001 aaaaaggaat caaccgtgag gacttattcc agctgatttc ctattcttat attttaaaag
     3061 ctgagaaggc tggactgatt tttcctagta tggagcagtc agtaaatagt gaaataggaa
     3121 aagtagctgg ctatggagct caattgaaga agtggtctat tcgaatccct cagaatgcct
     3181 cattctatag tacattttgt aaaatgatgg aaaattcaga agaaaatttt aaagcgatta
     3241 ttgatgaaga agtggggaga aaataatttc ccctctttct tttattttta acacaagtaa
     3301 aacccgaacg atatatttta attcataaaa taatttgaca aaaactgtac tttggtttat
     3361 aataaattaa ggaaacgtcg gaaaaggcgt agtgatgcaa gagcataggt aggtcattac
     3421 aaaagaaacg agacatcgat atgttaaatg aatttccaat ttttgattac gaagatattc
     3481 aattgattcc aaataaatgt gtcattaaaa gccgtgcaga agcggataca agtgtcactt
     3541 taggaaatca cacctttaaa ctacctgttg tgccagcgaa tatgcagacg attttggatg
     3601 aaaatgtagc agagcaactg gctaaaggtg gttacttcta cattatgcac cgttttgatg
     3661 agacaggacg cattcctttt attaaacgaa tgcacgatca agggctcatt gcttctatct
     3721 ctgtcggtgt taaggattat gagtatgatt tcgttagaca gctcaaggct gatgctcctg
     3781 agtatatcac tattgatatt gctcatggtc atgcggatag cgtgatttct atgattcaac
     3841 acatcaagaa agaattgcca gatacctttg tcattgctgg aaatgtggga acaccagaag
     3901 ctgtgcgtga attggaaaat gctggtgcgg atgctactaa ggtcggaatc ggtcctggta
     3961 aggtttgtat caccaaggtt aagactggtt ttggtacagg tggttggcag ttggctgctc
     4021 tacgctggtg tgccaaggct gcgcgtaaac cgattatcgc tgatggagga attcgtactc
     4081 acggtgatat tgctaagtct atccgcttcg gtgctagcat gatcatgatt ggttccctct
     4141 ttgcaggaca cattgaaagt ccagggaaaa cgattgaagt cgatggtgaa cagttcaaag
     4201 aatattatgg ttcagcctca caatatcaaa aaggtgctta caaaaatgtg gaaggcaaac
     4261 gtatcttgct tcctgctaaa ggtcatctgc aagacacttt aactgagatg gaacaagacc
     4321 ttcaaagtgc tatttcttat gcaggtggac gtcaggttgc tgaccttaaa cacgttgatt
     4381 atgtgatcgt gaaaaactcc atctggaatg gggatgcttc ccactaagac ggggtctttg
     4441 accaaaaaac ttgttattag agcaaatttc tgttataata aaacaagttt ccacccttag
     4501 tgtaatggat atcacgtaag attccggttc ttgagatggg ggttcgattc cctcagggtg
     4561 gatgtaaaca tcctaaaaag cctttaatag ggcttttttc tttatcctga cccaaatttg
     4621 ccgctaaaac tgagaatctc tgtctaaaga attcccaaat gctttccagt cccgttttag
     4681 agtgactcag ggggcttttt ttgatataat aaaaaggact attatcagtt agaaagaggt
     4741 tggtatgaaa gaattacaaa ctgtactaaa gaatcatttt gcaatcgaat ttgcagacaa
     4801 aaatttactg gaaactgcct ttactcatac gagttatgcc aatgagcacc gcctcttaaa
     4861 aatttcacac aatgaacgct tggaattttt aggagacgct gttctacagt tattgatttc
     4921 agaatatcta tataaaaaat atcctaaaaa gcctgaaggt gacctatcaa aactccgtgc
     4981 tatgattgtc cgtgaggaga gtttagctgg ttttgcgcgt gattgccagt ttaaccagtt
     5041 tatcaagttg ggtaaagggg aagaaaaatc aggtggtcgc aatcgtgaca ccattcttgg
     5101 tgatgccttt gaagcctttc ttggtgccct tcttttggat aaggatgtgg ccaaggtcaa
     5161 ggaatttatc tatcaagtca tcattcctaa ggttgaagca ggcgagtttg agatgattac
     5221 agactataaa acccatctcc aagagttgct tcaggtcaat ggtgatgtgg ctattcgtta
     5281 tcaggtgatt tctgaaacag ggcctgctca cgataaggtc tttgatgtag aagttcttgt
     5341 tgaaggtaag agcatcggtc aaggccaagg tcgttctaag aaattagcag agcaggaagc
     5401 tgccaaaaat gccgttgaga aggggctgga ttcatgtatt taaaggaaat cgaaattcag
     5461 gggttcaagt cttttgctga taagaccaag gtcgtttttg accaaggtgt gacggcagtt
     5521 gttggaccca atggatctgg aaagtccaat attacagaaa gtctgcgttg ggctttgggg
     5581 gagtctagtg tcaagagtct ccgtgggggc aagatgccgg atgtcatctt tgctggaaca
     5641 gaaagtcgca aaccgctcaa ttatgcttct gtagttgtga ctctggataa tcatgacgga
     5701 tttatcaagg atgcaggtca agaaatcagg gtggaacgcc atatctatcg tagtggagat
     5761 agcgaataca agattgacgg caagaaagtc cgtctgcgtg atattcatga ccttttcttg
     5821 gatacaggtt tgggacgaga ttccttctct attatttccc aagggaaggt tgaggagatt
     5881 tttaattcca agcctgagga gcgacgagct atttttgaag aagctgctgg agttttaaaa
     5941 tacaagactc gcagaaaaga aaccgagagt aaactgcagc aaactcagga taatctggac
     6001 cgcttagagg acattatcta cgagttggat aatcaaatca agcctcttga gaagcaagct
     6061 gaaaatgccc gtaaatttct agacttagaa ggtcaacgca aggctattta cttggatgta
     6121 ttggttgctc aaatcaagga aaataaggca gaactagagt cgacagaaga agagttggct
     6181 caggttcaag aactcttgat gagttattac caaaagcgtg aaaaattaga agaagaaaat
     6241 caaactctta aaaagcaacg ccaagattta caggctgaaa tggccaaaga ccaaggcagt
     6301 ttgatggact tgactagtct gattagtgat ttagaaagaa aattagccct atcgaaactg
     6361 gagtccgagc aagtggcctt gaatcaacag gaggcacaag ctcgtttggc tgctttggag
     6421 gataagagaa attcactcag caaagaaaag tctgataaag aaagctcttt agctctgtta
     6481 gagggaaatc tagtccaaaa taatcaaaaa ctcaatcgtt tagaagctga attgctggct
     6541 ttctcagacg atcctgatca gatgattgag ctcttacgtg aacgctttgt agctctttta
     6601 caagaagaag cggatgtctc aaaccagttg acccgtattg agaatgagtt ggaaaatagt
     6661 cgtcagcttt ctcaaaaaca agcagatcaa ctagaaaagc tgaaagagca attagctata
     6721 gctaaagaga aggctagtca gcaaaaggaa gagcttgaaa ctgccaagga gcaggttcag
     6781 aaattattgg ctgactacca agctattgcc aaggagcaag aggagcagaa aacttcctat
     6841 caagctcaac aaagccaact ctttgaccgt ctggataatc tcaaaaacaa gcaggccaga
     6901 gctcaaagtt tggaaaatat cctgagaaat catagtaact tttatgcagg tgttaagagt
     6961 gttctccaag aaaaagatcg cctaggtggg attattggtg cagtcagtga gcatctgacc
     7021 tttgatatgc attatcaaac tgccctagac attgcgcttg gagcgagtag tcagcacatt
     7081 attgtagaag atgagaatgc ggcaaccaaa gccattgatt tcctcaaacg aaacagagcc
     7141 ggtcgtgcaa cctttcttcc tttgaccact attaaggcgc gtacgatttc tagtcagaac
     7201 caagatgcta tcgctgtaag cccaggtttc cttgggatgg cagatgagtt ggtgactttt
     7261 gatactagac tggaagccat tttcaagaac ttgctagcta cgacggctat ctttgatacc
     7321 gtagaacatg cgcgtgcagc agctcgccaa gttcgttatc aggttcgtat ggtgacattg
     7381 gatgggacag aattacgcac gggtggttcc tatgcgggtg gtgccaatcg ccaaaataac
     7441 agtattttca tcaagccaga actggagcaa ttacaaaaag aaattgctgc agatgaagca
     7501 agcttgcgtt cagaagaagt ggctttgaag accttgcaag atgagatggc cagattgacc
     7561 gagtcattag aagccatcaa atctcaagga gagcaggcac gtattcagga gcaaggcttg
     7621 ttcctcgctt atcagcaaac tagtcagcaa gttgaagaac tggaaactct ttggaaactc
     7681 caagaagagg aaaaagatcg tctttccgag ggagattggc aagcagacaa ggaaaactgc
     7741 caagagcgtc ttgctgcaat cgccagtgac aagcaaaatc tggaagctga gattgaagag
     7801 attaagtcta acaaaaatgc catccaagaa cgctatcaaa acttgcagga agagctagcg
     7861 caagctcgtt tgcttaagac agaactgcaa gggcaaaaac gttatgaagt gactgatatt
     7921 gaacgcttag gtaaggaact agataatctt gatattgagc aagaagaaat tcagcgcctt
     7981 cttcaagaaa aggttgacaa tcttgagaaa gttgatacgg atttgctcag tcaacaggct
     8041 gatgaagcca aaactcaaaa aacaaatctc caacaaggtt tgattcgcaa gcagtttgag
     8101 ttggatgata ttgagggtca actggatgat attgctagtc atttggatca ggctcgccag
     8161 cagaatgaag agtggattcg caagcaaact cgtgctgaag caaagaaaga aaaggttagc
     8221 gagcgcttgc gccatctaca aagtcaatta acagaccagt accagatcag ctacacagaa
     8281 gctttagaaa aggcgcatga gttggaaaat ctcaatctgg cagagcaaga agttaaggat
     8341 ttagagaagg ctatacgctc actgggtcct gtcaatatag aagctattga ccagtacgaa
     8401 gaagttcaca accgtctgga tttcctaaat agccaacgtg atgatatttt gtctgcgaaa
     8461 aacctgcttc ttgaaaccat cacagagatg aatgatgagg tcaaggaacg ctttaaatca
     8521 acctttgagg ccattcgtga gtcctttaaa gtgaccttca agcagatgtt tggtggaggt
     8581 caggctgacc tcattttgac tgaaggagac ttgctgacag ctggggttga aatttctgtc
     8641 caaccaccag gcaagaaaat ccagtctctt aatctcatga gtggtggtga aaaagcccta
     8701 tcggctcttg ccttgctctt ctctatcatt cgagttaaga ctatcccgtt tgttatcttg
     8761 gatgaggtag aggctgcgct agacgaagcc aatgttaaac gtttcgggga ttatctcaac
     8821 cgctttgata aggacagtca atttattgtc gtaacccacc gtaaggggac catggcagcg
     8881 gccgattcta tctatggagt gaccatgcaa gaatcgggtg tttcaaagat tgtttcagtt
     8941 aagttaaaag atttagaaag tattgaagga tgacaattaa actagtagca acggatatgg
     9001 acggaacctt cctagatggg aatggacgct ttgatatgga tcgtctcaag tctctcttgg
     9061 tttcctacaa ggaaaaaggg atttactttg cggtggcttc gggtcgggga tttctgtctc
     9121 taggaaaatt atttgctggt gttcgtgatg acattatttt catcgcggaa aatggcagtt
     9181 tggtagagta tcaaggtcag gacttgtatg aagcgactat gtctcgtgac ttttatctgg
     9241 caacttttga aaagctgaaa acgtcacctt atgtagatat caataaactg ctcttgacgg
     9301 gtaagaaggg gtcatatgtt ctagatacgg ttgatgagac ctatttgaaa gtgagtcagc
     9361 attataatga aaatatccaa aaagtagcga gtttggaaga tatcacagat gacattttca
     9421 aatttacaac caacttcaca gaagaaacgt tagaagctgg tgaagcttgg gtcaatgata
     9481 atgtccctgg tgtcaaggct atgacaactg gctttgaatc tattgatatt gttctggact
     9541 atgtcgataa gggtgtagct attgttgaat tagctaaaaa acttggtatc acaatggatc
     9601 aggtcatggc ttttggagac aatcttaatg acttacatat gatgcaggtt gtgggacatc
     9661 ctgtagctcc tgaaaatgca cgacctgaaa ttttagaatt agcaaagact gtgattggtc
     9721 accataagga acggtcggtt atagcttata tggagggctt ataatggcag atataaaatt
     9781 gattgcattg gacttggacg ggaccttgct gactactgat aaaaggctga cggatcgtac
     9841 caaggaaacc ttgaaagctg cgcgtgatcg tggtatcaag gtcgtattga caactggtcg
     9901 tcccttaaaa gccatggatt tctttctcca tgagttaggg actgacggtc aggaagatga
     9961 gtataccatt acttttaatg gtggattagt tcagaaaaat acaggagaaa tccttgataa
    10021 aacagtcttt tcatatgatg atgtggcacg tttgtatgaa gaaacagaga aattatcact
    10081 gcctcttgat gccatctcag aaggaacagt ttatcaaatc caatcggacc aagaaagtct
    10141 ttatgccaaa ttcaatccag ccttgacctt tgttccagtg gactttgaag acttatctag
    10201 tcaaatgacc tacaacaaat gcgtgactgc ctttgctcaa gaacccttgg atgcagccat
    10261 tcagaagatt tctccagaat tgtttgacca atatgaaatc tttaaatcac gtgaaatgtt
    10321 gctagagtgg tcaccaaaga atgttcataa agcaacaggt ttggcaaaac taattagcca
    10381 tcttggaatc aaccaaagcc aagttatggc ctgtggtgac gaggccaatg acctctctat
    10441 gattgaatgg gcagggctcg gtgttgccat gcaaaacgct gttcctgaag tcaaggcagt
    10501 cgcaaatgtg gtaactccta tgaccaacga tgaggaagct gtcgcctggg ctatcgaaga
    10561 atatgtgcta aaggagaact aagatatggg attgtttgac cgtctattcg gaaaaaaaga
    10621 agaacctaaa atcgaagaag ttgtaaaaga agctctggaa aatcttgatt tgtctgaaga
    10681 tgttgatcct accttcacag aagttgatga agttcctcag gaagaagcag aggttgaaat
    10741 tgttgaacaa gctgtgttcc aagaagagga aatccaagac acagttgaag aaagtctgga
    10801 ttcagagcca gttgtagggg tttctcaaga agaagtcgaa gaatttccac actcagaaga
    10861 attaccagag gaagagaagc ttgatcacga aggaactata gaagaaaata attctgaagt
    10921 tcttgaagca gaaaggcctc aagcagaaga aaccgttcag gaaaaatatg accgcagtct
    10981 taagaaaact cgcacaggtt tcggtgcccg cttgaatgcc ttctttgcta acttccgctc
    11041 tgttgacgaa gaatttttcg aggaactgga agaactgctg attatgagtg atgttggtgt
    11101 ccaagtcgct tctaacttaa cggaggaact acgttacgaa gccaagcttg aaaatgccaa
    11161 gaaacctgat gcacttcgtc gtgtcatcat tgagaaattg gttgagcttt atgaaaagga
    11221 tggtagctac gatgaaagca tccacttcca agataacttg acagttatgc tctttgttgg
    11281 tgtgaatggt gttgggaaaa caacttctat cggaaaacta gcccaccgct acaaacgagc
    11341 tggtaagaag gtcatgctgg ttgcagcaga taccttccgt gcgggtgcag tagctcagct
    11401 agctgaatgg ggccgacgag tagatgttcc agtagtaact ggacctgaaa aagctgatcc
    11461 agccagcgtg gtctttgatg gtatggaacg tgccgtggct gaaggtatcg atattctcat
    11521 gattgatact gctggtcgtc tgcaaaataa ggataacctt atggctgagt tggaaaagat
    11581 tggtcgtatt atcaaacgtg ttgtgctaga agcaccacat gaaaccttct tggcacttga
    11641 tgcatcaaca ggtcaaaatg ccctagtaca ggccaaagaa ttttcgaaaa tcacaccttt
    11701 aaccggaatt gttttgacta agattgatgg aactgctcga ggaggtgtgg ttctagccat
    11761 tcgtgaagaa ctcaatattc ctgtaaaatt gattggtttt ggtgaaaaaa tcgatgatat
    11821 tggagagttt aactcagaaa actttatgaa aggtctcttg gaaggtttaa tctaatcaga
    11881 agcaaaaatc ctgcaaggca taaacttgca ggaaattttt ttattctaag cgaccatctt
    11941 gacgataggc gatatctggt tgccaagtcc atttggcacc gaatttttca agtaggtcaa
    12001 agctggcttg aggtcccatg cttccagctt tatagtcatg aagtggggca ccattttcag
    12061 cccagagctt ttcaatacgg tcaattaact tccatgacgc accaacttca tcccagtggc
    12121 taaagttagt tgagttgtta tttaggacat cataaatcaa tttttcgtat ggttctggag
    12181 aagcaccagt tgcagtcgca gctgtacggt aatcaagtga gttaggagcc aagttaaatt
    12241 cttctcctac ttgcttccca tttaggctaa gagagaagcc ttctgttggt tgaatataga
    12301 tggtcaaaat atttggagca agtggttctc caaatataga atccatttgt ttaaagacga
    12361 tgttgacatg agttcctttt tcagtcagtc gtttacctgt acggaaaaag aaaggaacac
    12421 tacggaatcg atcgctgtct acaaagaagg caccagatgt aaaggtttca gttgttgatt
    12481 ctggattcac atttggctcg ctacgataag agatgtattt catgccatca atcttaccag
    12541 agcggtattg cccacggata aagtgttctt tgagttcttc atcagttgga tgatagaggt
    12601 ttttaaagac cttaatcttt tcagcacgaa tctcgtcttt tgtgaagctt gctggtttgt
    12661 ccatggcgag gagcgaaaga agttgtagag tgtggttttg gaccatgtca cggagggcac
    12721 cggattggtc atagtagcca ccacgttctt ctacacccaa gcgctccgca aaggtaattt
    12781 gaacattgtc gataaaatcc ttgttccaaa cgttttcaaa aatcaagttt gcaaagcgaa
    12841 ctgcaaagat gctttggatc atttccttac caagataatg gtcgatacgg aaaatttgtt
    12901 cttcgtcaaa tgttgctagg agttcgtcat tcaacttgct tgcagttgcg taatctgtac
    12961 caaatggttt ttcaacgatc aagcgctcaa aacctttgcc atcgacaatg ttttcagatt
    13021 tgaggtgttt ggcaatggtt ccaaagaact gaggtgccat agacaagaag aagagcttat
    13081 tgtgttcagc ttggtatttt tcattaagct cagcttgtaa ttgacgcaaa gcaatataat
    13141 gttccgaatc attgacatca tggctttgat agtagaagtg gctagcaaat tcttgggctt
    13201 gctcggtact atctgccaaa tcaaggatgg actcgacaac tacagattca aaatattcct
    13261 tactccaagg tctacgggca gttccaataa cggcaaagtg cttggaaaga ttgccggatt
    13321 tatatagtct aaaaagggaa gggtagagct tgcgtttagc caggtctcca ctcgcaccga
    13381 aaattgtaac aataacctta gatgacatct agctacctaa tttctatttt tattcctagt
    13441 cttgctaggt atgaggaaac ctcatttcat aaacttaatt ctaacataat tttccgaaaa
    13501 atcaaaaatt ccaatacgag gtagaaaaac tgcaaggaaa tccttacagt ttgagtttag
    13561 acgtttaaga ccttatctaa gaactctttc agacgagggt gttgtgggtt atcaaagatt
    13621 tggtcaggtg ttccgtcttc aaggaactcg ccatctgcag taaagataac gcggttggca
    13681 acctgacgag caaatcccat ctcatgggtt acgataatca tggtcatgcc ttgctcagcc
    13741 aattccttca taacgttaag tacgtctcca accatctcag ggtcaagggc agaagttggt
    13801 tcatcgaaga gcatgatgtc tggattcatt gctaggccac gagcgatggc cacacgttgt
    13861 ttttgaccac ctgataggct atctggattg gcattagctt tatctgctag tccaaccttt
    13921 tcaagcaact ccattcccaa ttcctcagct tcttccttag tcatcaactt gtgctcaata
    13981 ggagcaaagg tgatgttgtc caatacagac atatgaggga agaggttgaa gtgttggaat
    14041 accatgccga tattttcacg gacgtggtca acattggttg ttttttcagt taaatcatag
    14101 ccgttcacag tgatgtgacc gctagtgact tcttctaaaa gattgaggct acggaggaaa
    14161 gttgacttac cagaacctga aggaccgatg atacaaacaa catctccttc atagaactta
    14221 gtcgtaattc cttttaggac ttcatttttt ccatagtgct tgtgtaaatc atttacatca
    14281 atttttaatt ttgccattaa cgaatcctct tttctaagcg tttcgctagt ctagtcaaaa
    14341 gtgtgataat tacaagatag aagatagcaa ggattgcata catcttgaaa ctttggtagt
    14401 tacgagcaat gataatctta ccagtttgga agagttcaac caaaccgata gcagatacga
    14461 tagttgtatc tttaagagcg ataacgaatt ggttgacaaa gtttggcaac atcaatttag
    14521 ttgcttgtgg caagataatc ttacgcatgg tttttccata agagataccc aagcttcggc
    14581 tggcttccat ttggccaact ggaacggcct gaataccacc acgaacgatt tcagcgatat
    14641 aagcagccgc attgagtgag agggcaattg ttccagctac aaagtcgtta attgggcttt
    14701 gttggcctgt gatagactcg atgaagtttg gaattcccca gaagatgaag gctgcaagaa
    14761 tcatcaatgg aataccacga ataacgtcaa cgaaaatctc agagatgacg cgaagagatt
    14821 tgtatgggct aacgctaaac ataccgaaga taattccgat gacaatggca atagcaaatg
    14881 agataagagc tagagcaaga gtgataccaa gaccgctaag gagttgtttg tagttgtttt
    14941 gaagcaagcc ccagagcgtt gtttcgtcaa cagtacttgt tgaagcagtt gaagattcgc
    15001 tagctaggta tttgtcaaga atcttttgga attcaccgtt tgctttaagg tttgcaagtc
    15061 cgttgttgaa catttcaatc agttctggat ttgctccttt tttaacggca aaggctgttt
    15121 caccgattgg agttccagag attggagttt tcaatttttg accttggctg atagaatatt
    15181 tgagaacagg ttcatcatcc ataacggcat caatggcacc agtatttaaa ctgtcataca
    15241 ttgaagaacc atcagcaaag gttttgattt tgtagccgta tttgctttga ttttctgtta
    15301 ggaaggtttg agaagcagtt ccgtttttaa caccgactgt ctttcctttt agatcttcat
    15361 aagaagcaat attgcttgat tctttgacac caagaatggt attagcagtg tagtatgatt
    15421 ctgagaagtc aaaagttgcc ttacgagcat ctgtgacaga cataccagcg atgataccat
    15481 cggcttgacc agcttggaca gcactgatag cagcatcaaa accagggttg gtgatttcaa
    15541 tttcaaaacc ttggtcttta gcgattgcct taatcaattc catatcaata ccagtgtatt
    15601 ggttgcttga attttggaaa acaaaaggtg caaaagaaga atcgctggca atgatatatt
    15661 tagccttaac aggaatggct tttaatcctg cgagagtagt tgtagttggc actgctgaag
    15721 atgatgaagc agtccatttc ttgataattt tatcaagact accatctttt ttcatttcag
    15781 acaaggcttg gttaaattca gtaaccaggt gctcgtattt acttcctttt ttcacaccga
    15841 aagcaaaact tcctacagct tcaccatcca tttcaatatg gaggtcttga ccttggttaa
    15901 tggcatattc gataacaggt ttgtcatcca tcatggcatc gatggcacca gcactcaagc
    15961 tgttgttcat taaatcacca gtgtcaaatg ttttaatagt aaagccgtat ttatctttga
    16021 ttgtttcaag gaaacgttga gcggcagttc cgtttttaac accaacggtt ttgccagtta
    16081 attggtcgta cttgctaatt ttgtgtgact ttgtagtagc aatgacaact tttgtatcat
    16141 agtaagtatc agacatggtg aagacttttt cacgttcttt agtctttgtc atccctgcca
    16201 tgatagcgtc ggcttgccca gcttgaaccg cattgactgc tgcgtcaaat ccaggatagg
    16261 acatctgaat gttccagcct ttaatctcag cgactttgtt aataatgtca acatcaattc
    16321 ctttataagt ttgatctgaa tctttaaact caaaaggtgc ataggcggta tcagaaacaa
    16381 tcttaatcgt ttctgctttg gcaataccta atgagaaaat tgggaataaa attagcaaaa
    16441 atgctagaaa ttttttcttc atttttagtc tccttttccg aatattctct cattatatca
    16501 gaaaaagtaa aaaaaggcaa atttttatat ttttcgtgtc agaaaatata acatcgattt
    16561 tttcctttcc ttgaattgct attaaaaagt gctataataa tagtatatat agaagaaaag
    16621 aggacgggga tatgaagaac aaaagaatat ttaaagactt ccaagcttca aaaatgagtt
    16681 taaacattta cacaagcccc ttgttagcct ttgtttttgt cttcatagga gagtttgtgg
    16741 cttttacttt gtatggtatt ggcttgttag ctctcatcgg acttgctaga aattttggag
    16801 aggctggtca aaatcttgca agctacttgc agaccttgca tcagagcttg acggataaaa
    16861 caagtgactt tcgtttaatt ttaggattac tggcctttgg ttttattctt aacactgtgt
    16921 tcagatggac aagaaaagtt gagaaaagac ctattcgaac cttgggattt tatagagaga
    16981 atttcctcag caatcttctg aaaggattta gtctaggcct ggcacttttt cttctgacct
    17041 tgttaggttt agtggtctta ggtcaatatc gtttggaatc cattcacttg aatccttatt
    17101 ctcttgcctt tgtcgtcttt actatcccat tttggatttt acaggggaca gcagaagaag
    17161 tggtggcccg tgcttggcta cttcctcaat tggcctcaag aaccaatcta aaactagcta
    17221 ttcttatatc tagcctgttc tttaccctgc ttcatatggg caattctggt ctcacccctc
    17281 tatctctagt aaatctcttt ttattcggag ttgccatggc tctttacctt ctcaaaactg
    17341 atacagtttg gggtgttgca ggtattcatg gtgcttggaa ttttgctcag ggtaatctct
    17401 ttgggatttt agttagtggt caaccgtcag gaacgtctct gatgaccttt ttaccacaag
    17461 gcaatcaaga ttggctatca ggtggttctt ttggcataga aggttccatt atgacaagtc
    17521 tggtattact actgctgatt gtctatcttg ctaataaatt aaagaaagaa aatgaaagga
    17581 tgtgacttcg gtccgtcctt ttcttcgtga aaatactata agtatgctaa aataggaata
    17641 gcacatggag agaggattct tatgatcaat cacattacag ataatcaatt taaactagta
    17701 tcaaaatatc aaccatcagg agatcaaccc caagctatcg agcagttggt ggataacatt
    17761 gaggggggag aaaaagctca gattctgatg ggggcgactg gaacagggaa gacctatact
    17821 atgagtcagg tcatttctaa agtcaataaa ccaactctgg ttattgccca caataaaact
    17881 ctggctggtc agctctatgg ggagtttaag gaatttttcc ctgaaaatgc agttgagtat
    17941 ttcgtatcct actatgatta ttaccagcca gaggcctatg tcccttctag cgatacctat
    18001 attgagaagg atagttctgt caatgacgag attgacaagc ttcgccactc agctacctca
    18061 gcccttttgg agcgtaatga tgttattgtc gtggcctcag tctcttgtat ctatggtttg
    18121 ggttcgccca aggaatacgc tgatagtgtc gttagtctcc gtcctggtct agagatttct
    18181 cgtgataaac tcttgaatga cttggtcgat attcagtttg aacgtaatga tattgatttc
    18241 caacgcggaa gatttcgcgt tcgtggggat gtggtagaga ttttcccagc ttcccgagat
    18301 gaacatgcct ttcgagtaga attttttgga gacgaaattg accgtattcg tgaagttgag
    18361 gctctgacag gtcaggtgtt gggagaagtg gatcatttag cgattttccc agcgacacac
    18421 tttgtgacca atgacgacca catggaagtt gccattgcaa agattcaggc cgagttggaa
    18481 gaacaattag ctgtctttga aaaggaaggt aaactgcttg aagcccagcg tttgaaacag
    18541 cggacagagt atgatatcga aatgttacgt gagatgggct ataccaatgg ggttgaaaat
    18601 tattctcgcc acatggatgg acggagcgaa ggagagcctc cttatacgct tctcgacttc
    18661 ttcccagatg atttcttgat tatgattgac gagagtcata tgaccatagg gcaaatcaag
    18721 ggcatgtaca atggagaccg ttcgcgtaaa gaaatgctgg ttaattatgg tttccgtttg
    18781 ccgtctgctt tggacaatcg tcctctccgt cgggaggagt ttgagagtca cgttcatcag
    18841 attgtttacg tttcagcgac acctggtgac tatgaaaatg aacagaccga gacagtgatt
    18901 gagcaaatca ttcgtccaac gggactcttg gatccagagg tggaagtccg tccgactatg
    18961 ggacagattg atgacctctt gggtgaaatc aatgcccgcg ttgaaaaaaa tgagcgtacc
    19021 tttatcacaa ctttgaccaa gaaaatggca gaggatttga ccgactactt caaggaaatg
    19081 ggtatcaagg tcaagtacat gcactcggat atcaagacct tggaacggac ggagattatc
    19141 cgtgacctgc gcttgggtgt ctttgatgtc ttggtcggaa ttaacctgct ccgtgaagga
    19201 attgacgttc ctgaagtgag cctcgtagct attctcgatg ctgacaagga aggtttcctt
    19261 cgcaacgaac gtggactcat ccagactatt ggacgtgctg ctcgtaacag tgaaggccat
    19321 gtcatcatgt atgcagacac ggttacccag tctatgcaac gtgctatcga tgaaactgcc
    19381 cgccgtcgga aaattcagat ggcctataat gaagagcatg gtatcgtacc acagacaatc
    19441 aagaaagaaa tccgtgacct gattgccgtg accaaggcag ttgctaagga agaggacaag
    19501 gaagtcgaca tcaatagcct caacaaacaa gagcgcaaag aactcgtcaa gaaactagaa
    19561 aaacaaatgc aagaagccgt cgaagtgctt gactttgaac tggcggctca gatccgtgat
    19621 atgatgttgg aagtccaggc cttggattag gggaatagta tgatttattt aagaaagtta
    19681 aagagagaag atttgatgtc tttatgggaa atggcttatt cacaacttaa tccagtttgg
    19741 aaacagtatg atgctcccta ttatgatgat tatcagtatt tttcaaattt taaagaattc
    19801 gaactacaaa aatcagaatc cattttaagc aactcaaatc gccttggtat ttttgttgat
    19861 gataaactag ttgggactgt ttcgcgttat tgggtatgta aacaaacaag atggatggaa
    19921 ttgggaattg gtatttatga taaaaaattc tggaacactg gtattgggaa agttgctatg
    19981 ttgcagtgga tagataggac gtttcaggat tacttggagt tggagcatct gggtttgaca
    20041 acttggtcag ggaatattgg tatgatgaaa cttgctgaaa aattaagaat gaaaaaagaa
    20101 gctcatattc caaaagttcg ttattatcaa ggta
//
LOCUS       DAVMEI010000020        19605 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVMEI010000020 DAVMEI010000000
VERSION     DAVMEI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.020"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(144..758)
                     /locus_tag="VYQ24_000583"
     CDS             complement(144..758)
                     /locus_tag="VYQ24_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1967759.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VYQ24_000584"
     CDS             complement(1573..1842)
                     /gene="rpsO"
                     /locus_tag="VYQ24_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1967760.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(1983..2612)
                     /locus_tag="VYQ24_000585"
     CDS             complement(1983..2612)
                     /locus_tag="VYQ24_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1967761.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            2849..3049
                     /locus_tag="VYQ24_000586"
     CDS             2849..3049
                     /locus_tag="VYQ24_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967762.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIVSLLYSYRFRFSL"
     gene            3480..3803
                     /locus_tag="VYQ24_000587"
     CDS             3480..3803
                     /locus_tag="VYQ24_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1967763.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDPLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            3861..5804
                     /gene="thrS"
                     /locus_tag="VYQ24_000588"
     CDS             3861..5804
                     /gene="thrS"
                     /locus_tag="VYQ24_000588"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1967764.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFEKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(5853..6827)
                     /locus_tag="VYQ24_000589"
     CDS             complement(5853..6827)
                     /locus_tag="VYQ24_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1967765.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(6820..7497)
                     /locus_tag="VYQ24_000590"
     CDS             complement(6820..7497)
                     /locus_tag="VYQ24_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1967766.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7653..8726
                     /locus_tag="VYQ24_000591"
     CDS             7653..8726
                     /locus_tag="VYQ24_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1967767.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEVFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            complement(8817..9119)
                     /locus_tag="VYQ24_000592"
     CDS             complement(8817..9119)
                     /locus_tag="VYQ24_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967768.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(9187..9624)
                     /locus_tag="VYQ24_000593"
     CDS             complement(9187..9624)
                     /locus_tag="VYQ24_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1967769.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(9700..10542)
                     /locus_tag="VYQ24_000594"
     CDS             complement(9700..10542)
                     /locus_tag="VYQ24_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1967770.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILELDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            10654..11304
                     /locus_tag="VYQ24_000595"
     CDS             10654..11304
                     /locus_tag="VYQ24_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1967771.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            11481..12127
                     /locus_tag="VYQ24_000596"
                     /pseudo
     CDS             11481..12127
                     /locus_tag="VYQ24_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            12258..12672
                     /locus_tag="VYQ24_000597"
                     /pseudo
     CDS             12258..12672
                     /locus_tag="VYQ24_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12691..13105
                     /locus_tag="VYQ24_000598"
                     /pseudo
     CDS             12691..13105
                     /locus_tag="VYQ24_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13144..13587)
                     /locus_tag="VYQ24_000599"
     CDS             complement(13144..13587)
                     /locus_tag="VYQ24_000599"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1967772.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(13617..15839)
                     /locus_tag="VYQ24_000600"
     CDS             complement(13617..15839)
                     /locus_tag="VYQ24_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1967773.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFDPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            16835..17464
                     /locus_tag="VYQ24_000601"
     CDS             16835..17464
                     /locus_tag="VYQ24_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1967774.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(17587..19479)
                     /gene="pepO"
                     /locus_tag="VYQ24_000602"
     CDS             complement(17587..19479)
                     /gene="pepO"
                     /locus_tag="VYQ24_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1967775.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         5734 a         4447 c         3642 g         5782 t
ORIGIN      
        1 ttataagaat ctcctaatca aatcaaaagc cactctacaa tagagtaact ttaagattgt
       61 ccatttatac tcaatgaaaa agccccctga aaatcagaga gctgatttga gctcgggcta
      121 aaatcctagt gaaacaaaaa aatctacacg atcagaaaag tctctatcgt gccattttca
      181 tacatgatgt atagtccaag tagaatgaat actaagggca caatgattcg ctcgtatttt
      241 tcaattgtct cgaatattaa cggaatagag gataacaccc gactaatctc gcaaaagata
      301 attatgccga ttacaaacac aagcaaggcc acgagggtct gtgaccaatc taacgaagca
      361 aaataaggta tatagatacc taaattatct ccgccagacg caattgtcag caatgtaact
      421 gtccaaaaca gttgatttgc cttgctttgt tctaatcttt caataatttc ttcctcttct
      481 tcttccgcat cttctccaac aattgcaaag cgaatcccta aatagatagg gattaaacca
      541 agcaatccaa ccatccattc ttcaggcacg aaattaacga cataagcagc aactaaactc
      601 gcccctacaa gtaagcctgt gcctagatat tgccccgcat aaatatgcca tttctgttta
      661 ttctgtgata gctgtgcaaa taaaataatt aaaataatta aataatcgat actggtggaa
      721 atataaacac caatagcaga tatgattgtc tgtcccataa aaaaaacctc ctgtattagt
      781 cagtaataaa tcagcagatt ttaggagagc acagacacat taatttagct atcgctagtg
      841 ggtaatgtcg aacgtaggaa agcattttac tcctcctcaa aacgtagtta caaagtaatt
      901 tctaactgga attcagtgac tacttccatg taaagtataa catactatac tttactttgt
      961 aaagagtggg ctcttctagg gggcttgcat acagctactc ggcttgaaaa gccgagtaac
     1021 tgcgcacctt tatggtgtaa ttagctgata ccatttgagg tttttgtagt ctccaaaatt
     1081 gttaccgata aaacctcaat aaaaaagccc tctgaagtca gagagctgat tttagcccgg
     1141 gctaaaatcc tagtgaaaaa gatgaaactc cttgtgctca tcgaacactg tgtcatttcc
     1201 ctattttcat acggattttt gacgccctta gcatcatgat tcttgctaga taaatcgttt
     1261 atagactagg ggacgaggtg aagatcgtac gaaactttca gtgaaacaaa aaaatctccc
     1321 cgaagggaga aaagctagtt tattttacct tacagtgcta ggaaccgtaa ccgaagatta
     1381 tacttgagta tagcgaggta aggtgacaac gcaacaaagc tacccaattt agagtagctt
     1441 tattatcaag atatgctcaa ttgaacacgg cctaagcact tggcaaaaaa gataaaatct
     1501 cctagaaact gaagtttctt cgtcaatttt cctatttttg ctttgtgttt ttgacggcct
     1561 ttgtatcttg aattagcgac gaagtcctag agagttgatc aactcacggt aacggttaac
     1621 gtcgttttta cgcaagtatg caagcaagtt acgacggcga ccgatttttt tcatcaatcc
     1681 acggtaagta gcgtggtctt ttttgtgttg tttgatgtgt tcgttaaggt ggttgatttc
     1741 ccaagtaagg acagcaactt gaacctctac tgaacctgta tcaccttcgt gacgtgcata
     1801 ttgtgcaatg atttcatttt ttttctcttt tgagattgcc atgatgtttc tcctttttat
     1861 ttggcttcat ccgagtgaca ggttggcatg cctgtaacca agaagaagtt atttgtcttt
     1921 acgacaatcc ctattttacc aagatttaaa ttttctgtca aacattaata ctctcatagt
     1981 tattattttt caagtaaaga gatacgttct tttatatgat gacctttttc aagttcatct
     2041 accaatccaa tagcataatc tgcataacta atctggctta ttcctttttc attagtagta
     2101 aaaatttctc ctgctagaat gtagtttcct gttttttcac catcgggaat aaaatctaca
     2161 gcaggactga caaaagtcca gttgagatta ttttttgtac gaagaagatc caattcatcc
     2221 gcttgagctt ttgctagtga cttaaattct tcaggaaaat cagacgtatc taaaagtcgt
     2281 gtcgtttttg tttcatctat ataaagactt cctgctcccc ctacaactag aaaacgtgtc
     2341 tgtgttccag caagaagctg attaaatagt tcgattgatt tgctgtggag cggtagcgta
     2401 tctggtgtat aagcaccaaa cgctgaaata acagcatcaa atccagtaag atcatctttt
     2461 gtcaactcaa ataaatcttt tttaataata gactcagatt gacttttgtt ttcagaacga
     2521 acaatagccg ttacttcatg tcctcgtttg actacttctt caacaattgc tttccccgct
     2581 tgtccatttg ctgcaataac tgctagtttc attttttata cctctcttat tgtaattatt
     2641 ttagttacaa caattgtgac actcttaata atcaatgtca atagttttgc ttaattatta
     2701 tcaaaatatt tctaccaaga aaactaacca tgattctagt gaaaaaaaat cttctttgcc
     2761 aacaaattta ctttcttgtt ttaaacatgc tataataatc atagcaagag atctaagttg
     2821 tctgtttttt taaaacgagg tgattatcat gcgtagattc tattcccatc tcccctacta
     2881 tctggtcata ttattctttt attggccact ttatgagttg ttcttactag ttgtttctga
     2941 cccccttaca ctcaaggaac tctatataaa caatcttctc ttctttacac ctctggtaat
     3001 cttgattgta tcgttactct atagctaccg tttccgtttc tcactttgat ggttagttgg
     3061 taacggactg ctcttttact ttactatcat aacctttggt gagtttatac taatttactt
     3121 gctaatctat gaaacagttg ctctggtggg catggattct ggtattagca tcaagcatat
     3181 tctacaaaaa atgaaaaaca aaaaactttc acaaaatcct tgaaaaatct cacaatcatg
     3241 ctataataat ccatagagac aagtcactta gtccctttct actagagagt gcgtggttgc
     3301 tggaaacgca taggaagtct aaactgatac tactcttgag ttttttatga aaacataaaa
     3361 cggtggccac gttagagcca atcagaggtg tccctctctt ttgaggtaca taaatgaagg
     3421 tggaaccacg ttgcgacgtc ctttcgagga tgtcgcattt ttttattagg atactaatta
     3481 tggagttgca agaattagtg gagcgcagtt gggcaatccg acaagcttat cacgaactgg
     3541 aagttaagca tcatgattcc aagtggacgg tagaagaaga ccccttggct ttatctaatg
     3601 atattggaaa tttccaacga ctggtgatga caaagcaagg acgctactat gatgaaacac
     3661 cctacacact ggaacaaaaa ctttcagaaa atatctggtg gctattagaa ctttctcaac
     3721 gtttggatat agacattctg acggaaatgg aaaacttcct ctctgataaa gaaaagcaat
     3781 tgaacgttag gacttggaag tagtctgatg ataaaaaatc aatgcttaga aactatgaaa
     3841 taataaaaaa ggagaacatc atgattaaca ttactttccc agatggcgct gttcgtgaat
     3901 tcgaatctgg cgtaacaact tttgaaattg cccaatctat cagcaattcc ctagctaaaa
     3961 aagccttggc tggtaaattc aacggcaaac tcatcgacac tactcgcgct atcactgaag
     4021 atggaagcat cgaaattgtg acacctgatc acgaagatgc ccttccaatc ttgcgccact
     4081 cagcagctca cttgttcgcc caagcagctc gtcgtctttt cccagacatt cacttgggag
     4141 ttggtccagc catcgaagat ggtttctact acgatactga caacacagct ggtcaaatct
     4201 ctaacgaaga ccttcctcgt atcgaagaag aaatgcaaaa aatcgtcaaa gaaaacttcc
     4261 catctattcg tgaagaagtg actaaagacg aggcacgtga aatcttcaaa aatgaccctt
     4321 acaagttgga attgattgaa gaacactcag aagacgaagg cggtttgact atctatcgtc
     4381 agggtgaata tgtagacctc tgccgtggac ctcacgttcc atcaacaggt cgtatccaaa
     4441 tcttccacct tctccatgta gctggtgcgt actggcgtgg aaacagcgac aacgctatga
     4501 tgcaacgtat ctacggtaca gcttggtttg aaaagaaaga cttgaaaaac taccttcaaa
     4561 tgcgtgaaga agctaaggaa cgtgaccacc gtaaacttgg taaagagctt gacctcttta
     4621 tgatttcaca agaagtggga caaggtttgc cattctggtt gccaaatggt gcgactatcc
     4681 gtcgtgaatt ggaacgctac atcgtaaaca aagagttggc ttctggctac caacacgtct
     4741 acactccacc acttgcttct gttgagcttt acaagacttc tggccactgg gatcattacc
     4801 aagaagacat gttcccaacc atggacatgg gtgacgggga agaatttgtc cttcgtccaa
     4861 tgaactgtcc gcaccacatc caagttttca aacaccatgt tcactcttac cgtgaattgc
     4921 caatccgtat cgctgaaatc ggtatgatgc accgttacga aaaatctggt gccctcactg
     4981 gccttcaacg tgtacgtgaa atgtcactca acgacggtca cctattcgtt actccagaac
     5041 aaatccaaga agaattccaa cgtgcccttc agttgattat cgatgtttat gaagacttca
     5101 acttgactga ctaccgcttc cgcctctctc ttcgtgaccc tcaagatact cataagtact
     5161 ttgataacga tgagatgtgg gaaaatgccc aaaccatgct tcgtgcagct cttgatgaaa
     5221 tgggcgtgga ctactttgaa gccgaaggtg aagcggcctt ctacggacca aaattggata
     5281 tccagattaa aactgccctt ggaaaagaag aaaccctttc tactatccaa cttgatttct
     5341 tgttgccaga acgcttcgac ctcaaataca tcggagctga tggcgaagat caccgtccag
     5401 tcatgatcca ccgtggggtt atctcaacta tggaacgctt cacagctatc ttgattgaga
     5461 actacaaggg tgccttccca acatggctgg caccacacca agtaaccctt atcccagtat
     5521 ctaacgaaaa acacgtggac tacgcttggg aagtagccaa gaaactccgt gaccgcggtg
     5581 tccgtgcaga cgtagatgag cgcaatgaaa aaatgcagtt caagatccgt gcttcacaaa
     5641 ccagcaagat tccttaccaa ttaattgttg gagacaaaga aatggaagac gaaacagtca
     5701 acgttcgtcg ctacggccaa aaagaaacac aaactgtctc agttgataat tttgttcaag
     5761 ctatcctagc tgatatcgcc aacaaatcac gcgttgagaa ataagagtct agcataaaag
     5821 cctccaatct ggaggctttt tctcatctat ttttactcaa ggactaagtt cacttgagca
     5881 aactgaatcc gcactgtcgt tccttttccg acctcagact cgatacgaat ctggtgcccc
     5941 agttcttcag aaattttctt agatagataa aggccaagtc cagaggactg ctgggtcaaa
     6001 cggccattgt atcctgaaaa gccacgttca aatactcgga ggacatcact gttttttatc
     6061 ccgattcccg tatctttgat acaaagctct tggtcatcca tataaatctc cagaccacct
     6121 tccttggtgt acttgagact gtttgagatg atttgctcaa taaccactag cagccacttt
     6181 ttatccgtca cgatttcttt atcaaggtca tgtagattga catttaagcc tttttgaata
     6241 aagaaaagag catatttacg aattatttcc ttgaccaagt cctcaatttg aacctgcttt
     6301 aagaccaaat catcatggaa actttctaaa cgcaggtact gtaaaactag gttggtatag
     6361 gagtcgattt tgaaaatttc ctgttctagc tgctgcttca gttggcggtc gaccacttct
     6421 gcaactaaga gttgactggc tgcaatgggg gtctttatct gatggaccca caaggtatag
     6481 taatccagca aatccgtcag ttttctttct gcttttgacc tctgctgata gagttccatc
     6541 tcacgcgctt ctaatttttc tgctaaagct atttccaaag gagacttggc ttccctctct
     6601 ccatagagaa gttcctggcg atagacctgc gtttccacca atatgtccca agtgaaaaat
     6661 aagatggtta caaagcaaca caagaagaaa aagtagagga agtaaattcc tagactggca
     6721 aataaaaact gaaagagtaa gacaagaaat gccaaagaaa gcagatagat aaaaagacga
     6781 ctacgggagc gcagataggc tagaaaaaat tgtttccaat caagcatgct tcaatccgta
     6841 ccctattcct ttcttggtct cgataaatcc taccaatccc tgctcctcca actttttacg
     6901 caaacgagcc acattgacag agagggtatt atcatcaatg aaaaagtcac tgttccaaag
     6961 ttcccgcatc aggtcgtcac gtgctacgat gttgcctgca tgctcaaata acacgcgtaa
     7021 aatctggaat tcattcttgg tcaaattcaa gacttgccct tgataatgta aatccatgga
     7081 tttggtattg aggataacac cagcatattc cagcaaactc tcatcacgcc caaactcata
     7141 ggaacgacgc aacaagccct gaaccttagc taaaagaacc tgctggtcaa aaggcttggt
     7201 cacaaagtca tccgccccca tattgattgc catgacaata tccatagcct ggtctctcga
     7261 agaaagaaac atgataggta ccttggaaat cttgcggatt tcctgacacc agtgataacc
     7321 attaaacaag ggcaaaccaa tatccatgag gaccagatga ggttccgact gaacaaatag
     7381 actcaaaact tccataaagt cttctaccag gaccacttca aatccccatt cagagagcat
     7441 tttcccgacc tgttgacgaa tgacctgatc atcttctatt aataaaatct tgtgcatgcg
     7501 cttctccttt tctattatta taacagattt ttccatgcca gatggtctga aactgaattt
     7561 gaaatagcct gtttttagcc agtacaaaca ggcaatgcta ctagctaatt tgagggaaat
     7621 ttgctaagat aaataaaaag aaaggagctc ttatggccaa tatttttgac tatctgaaag
     7681 atgtcgcata tgattcttat tacgaccttc ccttgaatga gttagacatt ctaaccttaa
     7741 tagaaatcac ctacctctcc tttgataatc tggtctccac acttcctcaa cgtcttttag
     7801 atctagcacc tcaggttcca agagatccca ccatgcttac tagcaaaaat cgccttcaat
     7861 tattagatga attggctcaa cacaagcgct tcaaaaattg caaactctcc cattttatca
     7921 acgacatcga ccctgaactg caaaagcaat ttgcggctat gacttatcgt gtcagcctcg
     7981 atacctatct gattgtcttt cgtgggacag atgacagtat cattggctgg aaggaagttt
     8041 tccacctgac ctatatgaag gaaattcctg ctcaaaagca cgcccttcgc tatttaaaga
     8101 acttttttgc ccatcatcct aagcaaaagg ttattctagc tgggcattcc aagggaggaa
     8161 atctcgctat ctatgctgct agccaaattg agcaaagttt gcaaaatcag atcacagcag
     8221 tttatacatt tgatgcacct ggtctccatc aagaattgac acagactgcg ggttatcaaa
     8281 ggataatgga tagaagcaag atattcattc cacaaggttc cattatcggt atgatgctgg
     8341 aaattcctgc tcaccaaatc atcgttcaga gtactgccct gggtggcatc gcccagcacg
     8401 atacctttag ttggcagatt gaggacaagc acttcgtcca actggataag accaacagtg
     8461 atagccagca agtagacaca acctttaaag aatgggtggc cacagtccct gacgaagaac
     8521 ttcagctcta cttcgacctc ttctttggca ctattcttga ggctggtatt agctctatca
     8581 atgacttggc ttccttaaag gcgcttgaat acattcatca tctctttgtc caagctcaat
     8641 ccctcactcc agaagaaaga gaaaccttgg gtcgccttac ccagttattg attgatactc
     8701 gttaccaggc atggaaaaat agataatact cttgaaaatt aaatgtatac aaaacaaaag
     8761 acctagaata catactttca tgtgcattct aagtcttttt aaatagaatc taatagtcaa
     8821 taaaaatcaa agagcattga gagataatgg ggcttggaac gtccctctcg cttcaacaaa
     8881 atgaccccat tatagattaa aaagatgcca cttagaaaaa gcaaaaaagg aagtaagaca
     8941 aaggcaaata cataaaaagc taactgaaca ttctcgtatc catttttata aaaaaggtag
     9001 gatagataaa aataacttga aatgagggat aataaaaata atactggatt ccacaaactt
     9061 ctattatcct tccaaaatga cactataaag gctaatacaa ttcctataac gagatacatt
     9121 tcttactcct ttaatagcta cattttatca taattatcca aagaaaaaag agggcattta
     9181 tccctcttaa tccttcatct gactctctgc atcggccacg actttttcta gactggtttg
     9241 accaagttct gcctccatag tcaactgaat tctctccaat ttttgatcca aaacatcatg
     9301 aatatgagct cctacagggc aatttggatt cggattgtca tggaaactga agagttgacc
     9361 tgtcttacca agacattcga ccgcctgata aacatctaaa agactaatat ccttaaggtc
     9421 cttgacaatc tctgttccgc ccgttccacg cgctactgaa atcagctctg ccttcttcaa
     9481 ctgggacaag atctttctga taatgacagg attgaccccg acactagcag ccagaaaatc
     9541 actggtcacc ttgctttcct tcccctcgag ggcaatgatt atcagcatat gagtcgcaat
     9601 ggtaaatcta cttggaattt gcatcctctt ctccttttta cgaggctacc ctgcctctac
     9661 tcttcttttt ctattattat acccttttta gttgtaatgt caatcgttac cacttttcaa
     9721 ccagtcgtct aactcccgat cgcagccctc tttctgagcc aattctctca aaaattcctg
     9781 atgatgagta tggtggatcc cattgaccag actttcatag taaacctcaa aatagggaag
     9841 tctcaggtct ttagccagct gcaattccgc tgccacatcg tagtctaccc gtcggaagtc
     9901 catatctacc aggcctttgt catcaagctc caaaatcata tactgggccc gcaagtcctt
     9961 ccgtagctga gcgtccaaaa agaaaggttg gccaatcgaa cccggattga caatcaattg
    10021 cccaccagtc ccgtaacgaa gcaactgctg gtgaatatgt ccataaacag caatatcaca
    10081 gggaggatga gtcaccaagc ggtcaaactc ctcttgtttg ccagtatgaa tcaactctcg
    10141 cccccagttc ttatcaggca gatgatggct aattcccacc gtcaaatccc caaactgacg
    10201 atgaatttga agaggttgat tgtggagcac ttcaatttct tctagggaaa tttcctctaa
    10261 aacatactgg cactggcgca agagatagcg ttgactgggg cgagtactgt ccaattcctt
    10321 acggacacca tgccaaagac tgtcttccca gtttcccaaa actctagccg taatcggtag
    10381 ttgatccaac aagtccaaaa tccttctacg tcctgtccct ggcatgagaa tatctcccaa
    10441 aagccagtat tcatccactc ctatctgctg agcatctgcc aaaacagcct ccaaggcggt
    10501 ggtatttcca tgaatatctg aaagaagagc tattttcgtc atatccatct cctcgttttt
    10561 tctcttgcaa taagtataac ataaaaagtc acagctagag aaatctagct ttttttgata
    10621 tactagataa agatattaga caagaggaaa cgaatgaccc caaacaaaga agactatcta
    10681 aaatgtattt atgaaattgg catagacctg cataagatta ccaacaagga aattgcggct
    10741 cgcatgcaag tctctccccc tgccgtaact gaaatgatca aacgaatgaa aagtgaaaat
    10801 ctcatcctaa aggacaagga atgtggctat ctactgactg acctcggtct caaactggtc
    10861 tctgagctct atcgtaagca ccgcttgatt gaagtttttc tagttcatca tttagactat
    10921 acaagtgacc agattcacga ggaagctgag gtcttggaac acactgtctc tgacctgttc
    10981 gtggaaagac tagataaact gctaggtttc cctaaaacct gcccccacgg aggaactatt
    11041 cctgccaagg gagaactcct cgttgaaatc aataacctcc cactagctga tatcaaggaa
    11101 gctggcgcct accgcctgac tcgggtgcac gatagttttg acattctcca ttatctggac
    11161 aagcactcac ttcacatcgg tgaccagctc caagtcaagc agtttgatgg cttcagcaat
    11221 accttcacta tcctcagtaa cgacgaggat ttacaagtga atatggacat tgcaaaacaa
    11281 ctctatgtcg agaaaatcaa ctaatttctc aagtccccta ccaaccctga aagttttatt
    11341 ttcagggttt tatttctatc atttgatttt actttcattt ggtggtagag tatttgaaaa
    11401 aaagaaaggt agtttaacct atgaaaaaat cactaaaaat ttttgctaca tctaaatgat
    11461 ttgacctctt cggggttgct ttggtcgttg ggattgcgat tgcatctggt tatctcaact
    11521 cacgcctcga taaatttgta gactggggac tatggacagc tcttgtaccc tttggattga
    11581 tttccgtaac caacgttggg atttccatgt tgtccactcg tttcacgggg aaattaagca
    11641 aatggggaaa ttactttggt attgttaata ccattttgtc cggtgctatt gattatatcc
    11701 ttggaaataa ggcggccatc attacctatc ccgtcacctt cctcatttat acctttgcta
    11761 taaagaaatg gaaagcttcg caagaaggca gacccaacca aatgagccaa aaacaggtaa
    11821 aattggcggc catcatcatt tccatcatcg ccttcctctt tgcctttgtg accaactata
    11881 tcggatatgg gggcaagatg aatctccttg cctacgtaac aactattgcc tttgcactgt
    11941 ccctcattgc caatgctttg aatgcattgg caatgaggga cagtggggct tttggttgat
    12001 ttacaatttc gttcagctga caaaagctgg tattcaagga aacttcgcca atatcggtaa
    12061 atacatcttt tatatcctca atgcaatcgg agctttattt gtctggaatg atgaagaagt
    12121 tagataatag aaggaaactc aaataaagaa tactagtcaa cttaagagaa tctcccctct
    12181 gctattttta tcattttagt tgtaactccc attcttttag agtagaatga agttagataa
    12241 aagagggagg tcttgtcatg aaaaaactct atagaatcca ttttatagca attgcagtta
    12301 ttgatttgct actatttgca ttttttatca ttagactcga gacatctttt gagtggctct
    12361 tgctctctgg tttaattttc ttccttgctc aaggactcct gctatttctc ttgatcgtcc
    12421 gactcaaaca tcaattcgct gagatttatc ctcaaatcaa taaaaagatt cgcttctact
    12481 atttaggggt tctcaccatt gattttctat ttttttgttc tcttagcctt cattagttct
    12541 cagcgttttt catctcttat gtcaattatc actgcttgcc attctacttt ttattatatg
    12601 acagctgact acctaagaga aaactatcca gacttttacg acaaacacat ctctttatgg
    12661 gagtgtctct aaggaaaagg aggttttatc atgaaaaaaa tcatcttcat caaaaccatt
    12721 caactccttg tcattgatgg aatcatgctg gcatttttga catttaaaag gggggcttac
    12781 ttgggactgg attttgattt atagcggttg gctcattttc tttcatcctg tgctattgac
    12841 ctatctttca aaccaacttt gtgaccactt tagtcaactc tattcccaga ttagaccgag
    12901 attctggcgt tttgctttac aaattctcct atgggatagc ctgatgattc tctccttggt
    12961 gtctttaagt gatattccac ttttccttca gggaactctc ctcatcctag gacatctcat
    13021 cccttcctat cgcatctgcc aaagcctgaa aagagacttc ccccaagcat atcaagaacc
    13081 gatttctttt tggagtattt tatgatagat gagaaagacc aagccgactg ggcttggtct
    13141 ttcttatctc tttttagtat ctaggataat ggtaacaggt ccgttattaa ccagctcaac
    13201 ctgcatatct gctccaaaga tacctgtctg aacgggcact tcttgcgcta atttttgatt
    13261 gaaagcatca tagaagtctg atgccatatc aggtttagct gcccctgtaa aggctggacg
    13321 attgcctctc ttagtatccg caaagagggt aaactgagaa atagagagga tttctccttc
    13381 aatatctttg acagacaggt tcatcttgcc ttctgcgtct gaaaaaatcc gcatattgac
    13441 cagttttctc acagcatagt ccaaatcttc ctcttggtcc tctggtccaa caccaactag
    13501 caataaaagt ccctgattga tttttccctg aatctggcct tctatactca cttgggcttt
    13561 tttaacccgt tggataatga ttttcataat agcctttcta gtaagagcta ggacaactag
    13621 ccgttggtcc gtttgacaga gtaaacttct ggcacactct taattttatc gacaaccgtg
    13681 gtcagtgtag agaggttggc aataccgaag gacacatgga tattagcaaa cttcatatcc
    13741 ttggttggtt gggcattgac cgttgaaata ttcttggttg tatttgaaag aacttgcagt
    13801 acatcgttca acagtcctgt acggttgaga ccgtagatat cgatatgggc catatactcc
    13861 ttatttgagc tagagtactg gtcttcccat tccacatcaa ggagacgttg ctcgtagttt
    13921 tcttgggcac gcaggttcat acagtccaca cggtgaatag ccacaccacg acccttggta
    13981 atgtagccaa caatatcgtc accaggcacg gggttacaac acttagcaat ccgcactagg
    14041 agaccagaag caccttcaat aaccactccc ccctcatgct tgaccttgag agtttcttta
    14101 ttttcaacct tgacctcgcc acctttgaca agctcctctg cctcagcctt ggccttggca
    14161 cgctcttcct cacggcgttc cttttcagtc agacggttaa agacggtaat cgcaccgatt
    14221 tccccaaaac caatggccgc aaagagggag tcttctgtct tgtaactggt cttttgcaga
    14281 acttgatcca tgtggcgctt gtccataaat ttatttgcca catagccatt ttcttggaac
    14341 tgagccatca gcatctcacg acccttgttg acagacaatt ccttatcttg gtttttaaag
    14401 aactggcgaa tcttattgcg cgccttgcta gtcttgacca tattgagcca gtcacggcta
    14461 ggtccaaagg agttcgggtt ggcgataatt tcaacctgat cccctgtctt taacttggtt
    14521 gtcagtggaa ccatgcggcc attgaccttg gcaccagttg ctttttcacc gaccttggta
    14581 tggatttcgt aggcaaaatc aatcggtcct gaatctttgg gaagggaacg gacagctcca
    14641 tctggggtaa aaacgtaaat ctcctcagcc agatagtttt ccttaacaga gtccacaaat
    14701 tccttagcat catcagcctg gtcttggagc tccatcatct ccttgatcca gttcattcca
    14761 atagctgatt ccttgctgtt aacttgcccc tttatacctt tcttataagc ccagtgagcc
    14821 gcaaccccgt actcagccac ctcgtgcatt tccttggttc gaatctggaa ttcaatcggc
    14881 ccttttggcc cataaacagt cgtatggata gactgataac cattggcctt gcggttggca
    14941 atatagtctt tgaagcgacc tggcatcggt ttccaaaatt catgcacgta accaagcatg
    15001 gcataaacat cactttgggt atctaaaata caacgaatag caatcagatc atagatttcc
    15061 tcaaaccgtt ttctcttgtc ctgcattttg cggaaaattg agtaaatatg cttgggacga
    15121 ccataaatct tccctttcaa gtgacgttct gtcgtatact cctctaattt tgtgactacc
    15181 tcatccacca aggcctcacg ctccctgcgc ttttccttca tcatatgggt aatcttgtaa
    15241 aactccgttg gattgagata acggaaagac aagtcttcta attcccattt gacactggaa
    15301 atccccaaac gatgggcaag tggggcatag atttccatgg tttctttgga aatacgctcc
    15361 tgcttgtctt ttcgaagatg tttcagggtc cgcatattgt gcaagcggtc agacagtttg
    15421 accaaaataa cgcggatgtc ctcagacatg gccatgagca tcttgcgatg attttccgct
    15481 aattgctcct cgattgattt gtactcgacc ttgccaagct tggtaactcc gtcaacaatc
    15541 acccgcacat caggatcaaa ctctctttcc aaatcgtcca aggtcgcatc tgtatcttcc
    15601 accacatcat gcaagaatcc acaagctact gttacagcat ccagctttag cttagctaaa
    15661 atacctgcca cttggatagg gtgaatgata taaggctcgc ctgatttgcg atattgacca
    15721 ctgtggcatt caacagcata gaccaaggcc ttatggacaa aatgaacatc ctcttccgtt
    15781 aaatattctt tggttaaagc gacaacttct tcgcctgtta aattcacttc tttcggcatc
    15841 tctactctcc aattcttcct accattttat cactttttta agaatatgaa aactagattg
    15901 gaacagaata agaaaaaaat aattcaaaat tgcttgataa ttctgaatta tcggcccata
    15961 atatactacg aagttagatt ttaaacttag gtgatagaag gagagataga agaacggaaa
    16021 ccatattgta acccaaagac tttctgattt ccccaattcc attgaagata cgaaagataa
    16081 acggtggaac tcgtatcaca tacactggta ccttgactgg attttggaat taatactcaa
    16141 tgaaaatcaa agagcaaact aggaaactag ccgcaggttg ctcaaagcac tgctttgagg
    16201 ttgtagataa gactgacaaa gtcagtcaca tatatacggc aaggcgacgt tgacgcggtt
    16261 tgaagagatt ttcgaagagt ataaaaatcc tcaagatact ttcttctatc ctttagttta
    16321 taaggagaat acctatgaaa aaaactgcta tttctatctt tgctctccta atgttaggag
    16381 tttgctgcct gttcctattc agccagcaaa gctataaaaa acagtcgttc aatactatgc
    16441 taacgaccag aacctgccca gtaggataac ttatagtgaa tatagcgaca aacgagaagc
    16501 caactacggt agcactctaa acatcacgtc tatcaaacaa gctaatgacg gagtttatgc
    16561 aacctatgaa gggcaattga cacctttcca atattgataa attgataacc agcctgtctt
    16621 catctagtca tgctggtttt taagttcatt ttaaatcctt acctattctc cctaactgtg
    16681 ctatacttaa tttatactca atgaaaatca aagagcaaac tagaaagcta gccgcaggct
    16741 gttcaaagca ctgctttgag gttgcagata aagttgacgc agtttgaaga gattttcgaa
    16801 gagtattagt acattctttg agattggagc tagtatgaaa atccataaaa ccgtgaatcc
    16861 tgttgcctat gaaaatacct attatctaga aggcgaaaag cacctcatcg tcgtcgatcc
    16921 tggtagtcat tgggaagcca ttcgtcagac aatcgagaag atcaacaaac cgatctgtgc
    16981 tattctcttg acccacgccc attatgacca tatcatgagt ctggacttgg ttcgcgagac
    17041 gtttggcaat cctcctgtct atatcgcaga gagcgaagcc agctggctct acactcctgt
    17101 cgataatctc tccggtctcc ctcgccacga tgatatggca gatgtggtca caaaacctgc
    17161 agaacacacc tttgtctttc acgaagaata ccaactagag gaatttcgtt ttaaggttct
    17221 accgacccca gggcactcta tcggtggtgt ttccctagtc tttcctgatg ctcacctagt
    17281 cttgacggga gatgctctat tccgcgaaac tatcggacgg accgaccttc cgactggtag
    17341 catggagcaa ctccttcata gtatccagac ccaactcttc accctaccaa actacgatgt
    17401 ctatccagga catggtccag ctactactat cgctcacgaa aaggccttca atcccttttt
    17461 ctagcaagat gatgacaatc gaaatttaag taaactatcc agcaaatctt tctattacaa
    17521 aaggcatcct atcaaggttt tcacacgtga ttggatgcct tttttctgat gactagattt
    17581 tttgtattac caaataatca cgcgctcctc tggtgaacgc cacattccgt ctccttcttt
    17641 gacatcatag gttgtaaaga aatcgtcgaa gtttggtact tgcacattga cacggagttt
    17701 ggctggtgcg tgcacatcga cgctagccaa aagtttcata aattctggtc gacctttcat
    17761 gcgccagatg cgaccgaagt tgtagaagaa ctcttctgct gagaagtctg cttctctctt
    17821 agctgcttca agcgctgctg cgattcctcc caagtcagcc acgttttctg atacagtcaa
    17881 tttaccgtta atggttgctc cataagaatc ctgtccatca aattggtcaa tgactttttg
    17941 tgttttctcc ttgaaggcag catagtcgct ctctgtccac caatccttga ggctaccatt
    18001 ttcgtcaaag gaagccccgt tagtatcaaa ggcgtgggaa atttcatggg caatcactgc
    18061 cccaatacca ccgtagttag cagaagatga ctgatgcaag tcatagaaag gcgcctgtaa
    18121 aatggccgct ggaaagacaa tcaggttctt ctgaggattg tagtaggcat tgaccatatg
    18181 agcaggcatg ccccattcct tatagtctac aggctggttc cacttactcc aactgtgctt
    18241 gatttccaca cgcgcaaagg ctagagcatt ctcaaaaaga ctggcagttt cattcactac
    18301 cttatccttg taacgtgcag gcaattcttc tggatagcca atataaggtt tgatcacatt
    18361 gagcttcacg atagcctgtt tacaggtttc tggagtgagc cagtcattct taagcagacg
    18421 ctccttatag acatcaatca tggttgccac ttttttctcc acatccgcct tggcttctgg
    18481 agagaacttc tcacgggcgt accaaagccc cagggcttgc ttgaaaggtt cctgtgctag
    18541 atgataagct gctttgacct tatcttttgc ctctggaact ccagaaaggg cacggctgta
    18601 ggcaccagac aaaacacgga tatcctctgt taaatagctg gttgaaagat tgacaacact
    18661 caaaatcaag gttgctttaa ggagagacca ggcttcctca ctgtaaaatt gctctgctgc
    18721 ttgccagaaa cgttcctcgt ctacaataac cttgtctggt aattgcccaa taactgcttt
    18781 gaagaagtca tccaaaggta gggcaggcgc gaatttcttg aaatcttcgt aagaatatgg
    18841 atgatagagt ttagcatatt ctgaactttc ttcattagag agcaccactg ccgcaactcg
    18901 gcggtccaat tcaagtcttt tttctagcaa gtcttcaatt tcttcatcag agaaatcata
    18961 agccttgagg agatttgcgc tgctttcttt ccaaagagtc aagagctctt cgcgctgagg
    19021 atgttcttct gcatagtagg tcgtatctgg caagattgtg cttggagcgc tagcccatag
    19081 aacattgatt ctagcatcca taaagtctgg cgatacacca aaaggaagga agtttggttt
    19141 tcctgcaagc tcaaactctg ctagtttagc tgtaaaatcc gcaaaagttt ccaattcttg
    19201 gaattcttta aggagtggta agacaggtgt gataccgtca gcttctctct tgtcaaaatc
    19261 acgaactagg cggtggtatt tgacaaagtt ttccaagata gcatcctcag gcacttcttc
    19321 acctgctaac cacttgtctg ttgtcgccag catcaggtct tcaatttcct ggtctaaatc
    19381 aacaaaacct cctgtttgag acttatctgc tgggatttca gctgtctgtt gccattctcc
    19441 attgatagca tcataaaaat catcttgata acgtgtcatc ttgttctcgc tttcatttgt
    19501 atttgcattt atcttaacaa aaatcactag ggaatgcaat taataaccat ttaactatta
    19561 ttttttagct attataaaaa atctttccat gataaaacgc ataat
//
LOCUS       DAVMEI010000021        18954 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVMEI010000021 DAVMEI010000000
VERSION     DAVMEI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.021"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(31..1362)
                     /gene="hk06"
                     /locus_tag="VYQ24_000603"
     CDS             complement(31..1362)
                     /gene="hk06"
                     /locus_tag="VYQ24_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1967776.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDENGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            complement(1359..2012)
                     /gene="rr06"
                     /locus_tag="VYQ24_000604"
     CDS             complement(1359..2012)
                     /gene="rr06"
                     /locus_tag="VYQ24_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1967777.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            complement(2654..5086)
                     /locus_tag="VYQ24_000605"
     CDS             complement(2654..5086)
                     /locus_tag="VYQ24_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1967778.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            complement(5088..5546)
                     /locus_tag="VYQ24_000606"
     CDS             complement(5088..5546)
                     /locus_tag="VYQ24_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1967779.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLVSVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            complement(5665..6393)
                     /locus_tag="VYQ24_000607"
     CDS             complement(5665..6393)
                     /locus_tag="VYQ24_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967780.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AETISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            complement(6393..7400)
                     /locus_tag="VYQ24_000608"
     CDS             complement(6393..7400)
                     /locus_tag="VYQ24_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1967781.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            complement(7438..8196)
                     /locus_tag="VYQ24_000609"
     CDS             complement(7438..8196)
                     /locus_tag="VYQ24_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967782.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            complement(8159..8449)
                     /locus_tag="VYQ24_000610"
     CDS             complement(8159..8449)
                     /locus_tag="VYQ24_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1967783.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     regulatory      complement(8520..8622)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8705..10051)
                     /gene="cbpD"
                     /locus_tag="VYQ24_000611"
     CDS             complement(8705..10051)
                     /gene="cbpD"
                     /locus_tag="VYQ24_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1967784.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     gene            complement(10140..10406)
                     /locus_tag="VYQ24_000612"
     CDS             complement(10140..10406)
                     /locus_tag="VYQ24_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967785.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            complement(10408..11760)
                     /gene="dnaB"
                     /locus_tag="VYQ24_000613"
     CDS             complement(10408..11760)
                     /gene="dnaB"
                     /locus_tag="VYQ24_000613"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1967786.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            complement(11804..12256)
                     /gene="rplI"
                     /locus_tag="VYQ24_000614"
     CDS             complement(11804..12256)
                     /gene="rplI"
                     /locus_tag="VYQ24_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1967787.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            complement(12253..14226)
                     /locus_tag="VYQ24_000615"
     CDS             complement(12253..14226)
                     /locus_tag="VYQ24_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1967788.1"
                     /translation="MKKFYVSPIFPILVALIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            complement(14362..14910)
                     /gene="raiA"
                     /locus_tag="VYQ24_000616"
     CDS             complement(14362..14910)
                     /gene="raiA"
                     /locus_tag="VYQ24_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1967789.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            complement(14990..15652)
                     /locus_tag="VYQ24_000617"
     CDS             complement(14990..15652)
                     /locus_tag="VYQ24_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1967790.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            complement(15649..16947)
                     /locus_tag="VYQ24_000618"
     CDS             complement(15649..16947)
                     /locus_tag="VYQ24_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1967791.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILAEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            17003..17638
                     /locus_tag="VYQ24_000619"
     CDS             17003..17638
                     /locus_tag="VYQ24_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1967792.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            17923..18843
                     /gene="cysK"
                     /locus_tag="VYQ24_000620"
     CDS             17923..18843
                     /gene="cysK"
                     /locus_tag="VYQ24_000620"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1967793.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
BASE COUNT         5652 a         4333 c         3146 g         5823 t
ORIGIN      
        1 tatgtgaact ttaataaaaa actaatctgt ctacaagcta atcttaaatt ccataccgtg
       61 ttcataaggg agaaaactat agtccatttg atagctttct aaaatattac tcacaatgta
      121 aagacccaaa ccggaacttt catccttatc tgtcacgatt tgagaatgat agaatatatc
      181 aaataaatgt tctagttctt ggtcacttag aggcgcacat gtattcttga tagagagata
      241 gccatcttgc tcggatatga ttacgcgccc attttcatct gaatatttaa ttgcattact
      301 aatcaggttt gtcaaaacct tatccaatgc cctaagactc atgacgaccc tggtagcatc
      361 tgtcaattga ttttcaattg taacacctat tgaatgagct aattcttgat aacgtgataa
      421 aatatctact aaaactggct taacagtcaa ggtctcacga cactctgtcc actcttggaa
      481 tttagaagac tccagtactt cttccaataa gtggctcatc tggtcaatct tatttatact
      541 ctttgcaata tattttggat gatctttgta atctccaata ttatgctgca tattctctag
      601 gataattcta agactggcta aaggggtttt caactcatgt gatgctccac ggacaaagga
      661 aaccttttga ttttgcaatt ttacaatttg ctcattacga ctttccaact catgaataac
      721 cttcaacaag tgctcataca taccattaat ctgttttcca acttcaccaa cttcatcttt
      781 cctactttca tcaaaacgaa tattgtcatc caaatcttgc attttactag tcacttctga
      841 aatgtaaaaa agaggattga gcaagcgttt agtataaaaa taagaaaaaa caaaggaaaa
      901 agcaattgta accaaaaatg tatatgggag atacaaaagc aaaatattct ttgcttcttt
      961 gtagacatcc accccgtgaa caaattgcac tgttacctgt ttaccatctg ctgtactaac
     1021 agacatatct aacatcacga tataatttga tagctttcca tctttaatat ccaagtcatg
     1081 cacaagaggg agccgcttat ctacaatatc tcttttaagg tgagcagata tggtcaagga
     1141 ctttgaataa gactggagta agcttggtat ttcatcagat gtcttagtct ttaaatactc
     1201 cgtaaacact cttgcgctct cattaaactt ttccccctcc agttgaatat aataaaaagg
     1261 aaaggtcaaa taaatagcta tatgaatgac tagaccaaca ccacctagaa ttgcaaaact
     1321 tcttaaaaaa gacttggtta ataatttagg attttttatc ataagctaat cttataccca
     1381 acatttttca cagtcacgat acaatctaaa gctaattttt tgcgaagagt acgaatatag
     1441 acatcaacga ccctatcaca aggtaaatca gctacatctt ttgaaatttc ttccaatatc
     1501 tgctctctac ttaaaacttg atttttatgc tgaatcaaac actttagtac cagtaattcc
     1561 tttggtttga gatcaatttc ttcattttta taatgtgctt tgtaagaggt aaaatccact
     1621 gttacatcct gatatcgcca aatatcctct atgacgtagt aacgtctgat aagcgcctta
     1681 atacgcttga caagcagtac caaagaaaaa ggttttgtca catgatcatc agcatagaga
     1741 tcaaatacct gtgactgagt agtttcatct cccagagcac tcaagacgat gacaggaata
     1801 tcgtgctttt gattgatttc ttttaacact tcaaaaccac tcttcctagg catcattaaa
     1861 tccagtacca tgagatggat agggagatct tgaaattttt ccaagacctc ttgtccatcc
     1921 ttagccataa tgacatgata accctcttct gtcagaaatg ctgcaattcc ttctctaatc
     1981 atttcctcgt catctgcaac taaaatgttc atctctctcc ctttctacta ccagaactcc
     2041 atacatctat ttcctgtatg ctatttgaaa atttcctacc agcctatcat ctatgataat
     2101 ggaatggatt tcatttatcc gctacttttt gtcaacaaca acgtcaatac aagaaaatca
     2161 ttcatcttgc agcttatact cttcgaaaat ctctccaaac cacgtcagct ctatctacaa
     2221 cctcaaaaca gtgttttgag caacctgcgg ctagctgcct agtttgttct ttgattttca
     2281 ttgagtatta gtttgatttt tcaaatacca gttagaacgg atttgacatg caaaaaagaa
     2341 acaggtctct tttgaaagaa taaaaatttc agataagact gctctttttc tactacacac
     2401 gctctaagac aaatacgacg gagggtaata aacatatgaa ttttgtgttg aatggtgaaa
     2461 accacgaatc gtttagaagc tataggtttt agtttcatac tcttcgaaaa tctcttcaaa
     2521 ccacgtcagc tctatctgca acctcaaaac agtgttttaa gcaacctgcg gctagctgcc
     2581 tagtttgctc tttgattttc attgagtttc actccactgt gacttatggt cagatgctga
     2641 tacttttatt cttttatgca atatcaaatt ttaactggcc tgctttgaca ccaatcttaa
     2701 gtgtgctgcc tgccactaaa tctcccttaa gaagaagttc tgccaacttg tcctccactt
     2761 ctgtttgcag ggttctgcga agtgggcgag ctcccatctc tgggtcatat ccttgatttg
     2821 ctaacaattt cagagctgaa gcttgtaatt tcaagtcaat gcctttttca gccaaacttg
     2881 ccactaaagg cttgaccata atcttcacca cttcctgcat atggtcacta gataggctat
     2941 ggaagaccac cttctcatca atacggttga tgaattccgg tctataagct tttttcagtt
     3001 cttcaaacat gcgtttttcc atattttcct ggtcaaaacg aatatcctta gccccaaaac
     3061 caacagtctt atcatcacga agggcagtcg cacctagatt cgatgtcata atgataatgg
     3121 tatttgaaaa atcgaccttg cgtcccttgc tatctgtcaa gacaccgtca tccagaacct
     3181 gcaagagaac attaaagata tctgggtgag ccttctctac ctcatcaaag aggagaacgg
     3241 aatagggttt attgcgaacc ttctctgtca actccccacc ttcttcgtaa cccacatagc
     3301 ctggaggagc tccatttaga cggctggctg cgaatttctc catatactca ctcatatcaa
     3361 agcggataag ggctgattcg tcatcaaaga gaacttctgc caaagccttg gccaactcgg
     3421 tcttaccgac acctgtgggt cctaggaaca taaaggaacc gatcggacgc ttgtgactgc
     3481 gaattcctga ctgattacgg cgaatcgcac gactaatact tgaaacagct tgatcttgac
     3541 cgataacccg tttatggagt tctgcttcaa gatttagata tttcttagcg tcagtctgag
     3601 tcagtttttg aactgggatt cctgacaagc gactcaaggt ggtcaaaata tcagactctg
     3661 tcaccaagtc tttgtagaca ggtacttcct cttcttttgc gattagctgg gctgcctgtt
     3721 tccacttgcc atccatcagg gccttgtcag ctggactcaa atctgaatcg tctgctttta
     3781 catactttgc cttattttgc actgttgctg ctgcttcgtc caagaggtcg atagcagagt
     3841 ctggcaagtg acgactggtt aaataacgat gagccatctt aaccgctgtt tcaaccgctt
     3901 catctgtgat ttgtacacgg tgatgtttct cataagtcgc cttcaaacct tgtaaaatag
     3961 tcatactatc tgccacactt ggttcttcaa tcgtcacttt agcgaaacga cgagaaagag
     4021 ccgcatcttt ttcgatatgt ttttgatatt cttcctgagt ggtggcacca accgttctca
     4081 aagttccacg cgccaaggct ggtttcaaga tattggccgc atccagagtc gaatcaattc
     4141 cgctaccaga acccatgatg gtgtggagtt catcgataaa gaggatgact tggccatctt
     4201 cttcaatatc cttgatgata ttattcatgc gttcttcaaa gtcaccacgg aagcgtgtcc
     4261 ctgcaacgac attcatcaaa tcaagttcta acacgcgcat cttagccatt tccgcaggca
     4321 cgtcaccact agcaatacgc tgggcaagac caagcgccag agctgttttc ccgacaccag
     4381 catccccaac caagacaggg ttgttcttag tcttccggct caagatttga atcatacgtg
     4441 agatttcctt gtcccgaccg atgactggtt ctaacttgcc agaacgcgct tgctctgtca
     4501 aatcatgcgt ataatcctcg agaccaccac taggagtctg cggcatgccc atcatattgg
     4561 ccatagaatt ttgcttgtca gctactgtac gatggcgttg gcgtaaagcc ttgagatctt
     4621 cacgagtcca gcctgcccgt tcttctaaat ttcgacgaag agcagcaatc ttgacctgat
     4681 ctttcttgtc ttcataagaa aaaccagccc tctccaagat acgagttgcc aaggcattgc
     4741 catcatgcaa aatcgcatag aagacgtgct ctgtccctag caccttagca tggaccactg
     4801 acgctacata ctctgcttcg tcaaaaagaa cctgcaaacg acgggagaac ggcaattccg
     4861 taaaggtttc atcctggcta tagtccgttt cagtcagttc caaagccacc tcttctaaac
     4921 ggtccatctc atacggataa tcatttaaag ttgcccctgc tacactataa ctgtgattag
     4981 acatggcaat caacaagtgc cacgactcta gataacgagc tccaaaatgt ccagcaacca
     5041 tgtaggcact ttcgatacat tcattcaatg cttttgaata gttcatctta cttccctttt
     5101 ctatctacct cttgtatgac ctgacgtagc atgtttgctc gaacaactgg agcttcttct
     5161 cctaaaacgc gatccaaagc tactgatact agcaaattca tctcctgctt ggtcatcaat
     5221 tcctgctcaa ccaaaagctg gagaatatcc tcataaattt cttgactgac tcgctcacca
     5281 atcgagtaaa gcagctcccg gagcatttca tgatgactag aaaactcaat ccgtcctata
     5341 cgaatgtagc ctccgccacc acgcttactt tcaaccaagt agcctctact ttccgtaaag
     5401 cgtgtcttga tcacgtagtt aatctgacta ggaacaacct gaaaggtatc tgccaactga
     5461 ctccgttgca actccacgat accagattga tctaaaatcg ccttgatgta ggcctcaata
     5521 tgatccgatg tatttttaaa tctcataaca aacccacctc tctctttaaa ccttgacctt
     5581 gtttgactat tataccgaaa ttttctcatt tttaaaagaa aaagggcgct ggtaaaggat
     5641 aatcttcacc aactccctat ttttctactt atctaagcct aattctgcca agatttgacg
     5701 tttgtaggca atcttttgga cttccttgtc ctcatcttca gaccaatcta gtttaatttc
     5761 tgaaacaatc tgcccagggc gatttttcaa gatatagatg cggtcgctga gattgagggc
     5821 ctcctcaata ctatgcgtga tgatcagggt tgttagctgc aactgcttgt gaatctcaag
     5881 ataccaagcg tggagttcca tctttgtcat ctcatccaag gcgctaaagg cctcatctaa
     5941 gagaaagagc ttatgcccaa aaaggtaggt ccggagtaag gctacacgct ggcgcatccc
     6001 accgctaagt tcatgaggat acttgtctct tacagctgtc agctggaagg tcgcaagaat
     6061 tttatccgct cgggaaatag tttctgcctt atccaccttt tgaatcaaga ggggtagaat
     6121 gatatttcca agcaccgtct tgtgctccaa gagcagatcc ttttgcaaca tataactcac
     6181 gcgccccttg ggattttctt caccatcaag gacaattctc cctgactgaa cttctaaaat
     6241 cccagcgatt agattaaaga gggtggtctt tccaacacca cttgggccta ggatggaaac
     6301 cacttcgcct gaagtcacct gtaggttgat atcctctaaa atcctctcct gaccataggc
     6361 ataactgacg tgctctagtc taatttctgt cattatttca caaattcgtt ggtgaagcct
     6421 ttgtctgtca agtcttcttt aaggatacca ttttctttat cccatttata gaaagcattc
     6481 cagcgagctg cgtcaaattg accccatttt tccttgtcgc ttgcgtattc ttttgacaag
     6541 tatttttgag attcgatgac aaagtcacgt ttttccttga gttcaggtgc attcttgatg
     6601 agaatatctg cagcttcttc tggatgttcc atggcatatt ggtagccttt tttgatggct
     6661 tggatgactt tgcgagcttc ttctttgtta tctttcagat agtcgttgtt tgcgatgata
     6721 actggtgaat agtagtcaaa ctccttgaca tagtctttca agtacatgaa gttagcatct
     6781 acaccttgag atttagcaag gataccatcc caaccgtagt aaatccaagc agtatcaaag
     6841 acgccattgg caatcggtgt gattgagttt gagtcgttat ttggtacttt ttcaaccttc
     6901 tcaaagtctc caccttgaga ttctaccaag gttttcaaca tagcaagttc agttgggtca
     6961 ttccatgtcc catatttctt accaaccaag tcttttggac tgcttacatt atcagattta
     7021 cgagagatga ttcctgatgt attgtgttca acaatagctg caacggcagt gattcctgct
     7081 cctttttcca atttcttagc catgtagtct tggaaataca ctgcaaatgg tgcctttccg
     7141 ttgataacca agtcagaaga actttcttct ggtggcaatt tcaaatcaac atccactcca
     7201 gcttctttga aataaccttt ttccttggca acataaagcc ctgtgtggtt ggtatttggt
     7261 gtccagtcta ggataaagtc aaccttctta agttctgcct ctttgttgtc tttagaagca
     7321 gttccttgac cacaggccac aagcacaaca gctacaagag ctgttacaag cgttaaaaac
     7381 actttccatg ttttcttcat tttgatttcc tcttttcttt ttcaaaaacg ctctatttta
     7441 agtacgtttc catttaatca catatttttc actgatatcg accagcttca tacccaaaag
     7501 actgataatc gacaccagaa taataatggc aaacatggta tcatactgaa acagtttttt
     7561 agactgaatc atataaacac caagaccttc aaaacctccc aaccactcag ataccacagt
     7621 tgtgataaag gcgtaggaga cactgaccct cagacctgca taaaagtaag gcaggctaac
     7681 cgggatttta aaatgccaca ggatttgcca agggtttgct cgcatcagac taaacaaagt
     7741 cagcatatcc ttgtcacaat gcctaaaacc gtccaaaata ctaacgatga tgggaaaggt
     7801 tgttgttaag ataatcaaga caatcttggg caaaatccca taacccagcc acaagaccag
     7861 gataggagct atggcaatgg tcggaatggt ctgaatgacc accatcatag ggtaaatcag
     7921 gtcattgagc caagtcaaac tatccatgag cacagccata agacaggcaa tcaaaactcc
     7981 caaaatcagc cccagtaaag ccactctcaa ggtcgcccag ctatggtgcc agagaaattc
     8041 tctgtcacga acaaagggct ggagaatttc aagaggtgtc ggcaggataa acttggggag
     8101 aagtttaaga aaacctgcta actgccagat tgacaatact ccgagaaagc ccaatagact
     8161 aatgtgtcgt ctcagtatac ttttcaagtt tctcatcaat acttaaaatc tctcctacat
     8221 ttattttgac attggcaaag acattgtctg cctcctgccc tgccacttcc agcgcttctt
     8281 ttagaatgcg cataagctca tcaaactccc cttccaagac cgtttcaaat ggtgtcacta
     8341 ccatcgtcac ttcttgagtt tgcagataag caatgacctg atcaataaca gctatccgat
     8401 caatcccctg tgctaggggt aaaacttgca aggcaatgct tgctttcata agaacctcct
     8461 cttctcgttt tccttttaaa aaaaagaaaa acctttacca tacatggaaa aggtgcaaaa
     8521 ttagactaag tatcgttccc tccgctggca ttacccagat caggtgcggt cgaagtttaa
     8581 cacttcctct cagactgtac acagactccc atatattata tgtatatatg atagcgcaaa
     8641 ataagaaaaa tgtcaaggaa actgcttaca gaaaagagca ggaaatttct cctactccaa
     8701 ttttctatac tcgttctcca tcactgttta ctctgtagcc atccaccgtc gtattaacag
     8761 ctaaggcgcc tgaagagtaa gaataatacc acttaccaga gacctggtac cagcctgttt
     8821 tcattgcccc tgaactaccc agataatacc acgagccatt ctcctttaac catcctgtct
     8881 gcatttcacc agataatttc aaataatacc agctagaacc gtctttcagc catcctgttg
     8941 atttagaacc atttgatttg aaatgatacc aactaccatt tattttcttc caaccgacag
     9001 ttgaactatt attggatcca tagctagaga tatttcctga ttgattctga taattttgtg
     9061 aagaagtcac tccctctagc tgtatatagc gacgacttcc attataggca gtataactca
     9121 accacttgta gccgtctttt tcaagtatct gatcataatg aaccttctcc ccaggataat
     9181 agtaatcaat cacagttgca ctaactaggg gttcagtttt gatagcagac ttggtcttaa
     9241 aataatgagt tccacctgtt gaagaaagaa cagaattacc tagaggattt ttattcacag
     9301 cctccaattg aacataacga tagcttccat tataggcagt ataactcaac cacttatagc
     9361 cgtctttttc aagtatctga tcataatgaa ccttctcccc aggataatag taatcaatca
     9421 cagttccgct agctaggggt tcagttttaa tagcagactt ggtcttaaaa taatgagttc
     9481 cgcctgttga agctgaggat tgactattcc caacactgcc gccatccaaa tctttaaaat
     9541 gaataaatcc tgtcatcgtg tttgctttta taactcgttt attataggat tctgtataac
     9601 catagttata ttcctcaatc tcaatctgat ctcccattac atttgacacc caggcaacat
     9661 gaccataagt tcctgcagta gaccaagtaa tggaaccaat cgtcggtgta ttatctacac
     9721 gataaccttc ccgacgagca cgatgtcccc attcattcgc atttccataa gctgccggaa
     9781 tttcaaaacc attgacatta ctcaaacgaa aggctacaaa agaagtacac tgacgagaat
     9841 acatgcgcca ctgatcaatc tcctggctcc catttttata ataagcagga taatcatccc
     9901 cacgcgcaat cgatccattt cctctggaat aggcatagac actatcacca gctgctagca
     9961 tcaatcctac tactaaaaaa ggaacaagct ttttaactga catcttcaag taataacttg
    10021 ttcctcttgc tataaacggt aaaattttca ttcttcctcc ttgaaaaata atataaagcg
    10081 atgattgcct tcacctaaat tattcggaga aaaaagaaaa agtgagaaaa tttctcactt
    10141 tagtcaagat aatgaatcaa attcttttct gtaagaatat ctgagtaagt aaaggattca
    10201 acgtaaacat taacttgttt atctccttcc acatccccaa actccacaat aaatagagat
    10261 ggataaactt caattagctt acccaatcta tttttttggc gcttacgacc attctccaaa
    10321 gtcatttcta cgacttgtcc ctcatgtgcc ttgatttctt ctttgatttt tttcatcttg
    10381 gctacatctg taaatgcatc tgacatctta tgcctccctc tttgagatac ttgaaaattt
    10441 attgtattct ttttggaaaa tcaattccac tgttccacga gctccactac ggtttttctc
    10501 gataataact tccaccttat tatttgggat accctcctct tcttcaccac cacgttcata
    10561 gtagtcatcg cgatagagaa aagctacgat atcagcgtcc tgctcaatag acccagattc
    10621 acgaatatca gacaagaccg gtctcttgtc ctgacgttgt tctacaccac gagaaagctg
    10681 actcagagcg attactggaa ccttcaattc cttggctagt attttcaact gacgagaaat
    10741 ttcagaaact tcttgttgac gattttctcg accagttccc gtgataagtt gcaaatagtc
    10801 tatcaaaatc aaaccaagat ttccagtttc ttgagccaat ttacgagaac gagaacgaat
    10861 ctctgtaatc cgaatacctg gcgtatcatc gatatagata ctggcgttag ctagattacc
    10921 ctgagcaata gtatattttt gccactcctc atctgtcaat tgccctgtac ggatagaatg
    10981 tgactccact aagccttctg cagctaacat acgatctacc aagctttccg cacccatttc
    11041 gagtgaaaaa atagcaaccg ttttgtccaa cttagtccca atgttctgag cgatattcaa
    11101 ggcaaatgct gtcttaccaa ctgctggacg agctgctaag ataatcaact cctcctcatg
    11161 aagtcctgtt gtcatatgat ccaaatcacg ataacctgtc gcaatacctg taatatcggt
    11221 cgtttgttgc gagcgagctt ccagatttcc aaagttgaga ttcaacacat ctcgaatgtt
    11281 cttaaacccg cttcgatttg cattttcact gacatcaatc aacccttttt ctgcctgagc
    11341 aataatttca tcagctggtt gtgacgcttc gtaagcttgg ttgacagact ctgtcaactt
    11401 ggcaattaaa cgacgtagca ttgctttttc tgcaacaatc ttagcataat actccgcatt
    11461 agcagaagtt ggcacagaat tgacaatctc aaccaagtaa gacaagccac caatattctg
    11521 taaatcacct tgattatcaa ggatagtacg aaccgttgtt gcatctatgg catcaccacg
    11581 atcggataaa tcgaccatgg cttggaaaat caaacgatgg gcatacttaa aaaagtcccg
    11641 agactcaatg tattctcgca caaaaacaag tttactctca tcaataaaga tagcccctaa
    11701 aacggattgc tcagctaaga tatcttgagg ttgtactcgt aactcttcta cttctgccat
    11761 cagacttccc ttccttttac aatcttgtca agaaggtgta aacttatcct tctttcacac
    11821 gaagattgat tacacttgtg atatcttgat agattttcac tggcacatca atcaaaccaa
    11881 ccgctcgaat cggagcttgt acttgaatat gacgtttatc aatcttaatt ccaaattgct
    11941 tttgcaattc ttctgcaatc ttcttattgg taatagaacc aaaggtacga ccatctggac
    12001 caactttttc aacaaattct acaacagttt cttctgcttc aagttgtgct ttaattgctt
    12061 ttccttctgc aatcatctca gcgtgagctt tttcttccga tttttgttta ccacgaagtt
    12121 cacctacagc ttgagcagtc gcttctttgg ctagattctt tttgataaga aagttttgcg
    12181 cataccctgt tggtacttcc ttaatttcgc cttttttacc ttttccttta acatctgcta
    12241 aaaagattac tttcattctt ctttctcctt ttcctttatt tcatttaata caatttctgt
    12301 cagtttttca cctgcttctg acaaggttac atctttaatt tgagctgctg ccaaattaaa
    12361 gtggcctcca ccgcctaact cttccataat ccgttgtaca ttcagtttac tacgacttcg
    12421 agctgagata gagataaatc cttgtgtatt cttcgcaaga acaaaactcg cttcaatacc
    12481 tgacatggct aacatggcat ctgctgcctt actaataaca actgtatcat agcatttcgt
    12541 gtccttagcc tctgctatta gtacatctga acctaattta cacccctgta aaataagttc
    12601 attgacctca cgatattctt caaaatctgt cgcagcgatt tcctggatag caatactatc
    12661 acttccgcgc gttctgagat agctagcaac atcaaatgtc cgactagtta ctcgcgaggt
    12721 gaaattttta gtatccaaca tcataccagc catcaaaaca cttgcttgca tacgactcaa
    12781 acgatttttc ttagaattct ggaactgaat caattccgtt accaactcac tggcactact
    12841 tgcaccactt tcgatataag taataaccgc attatctgga aaatcctgat cccttctatg
    12901 gtggtcaata acaatggttt gggtaaataa atcataaaat tcttttgata atgttaaggc
    12961 tgtctttgaa tggtctacaa gaatcaacaa agaacgattg gtcaccatcc ccattgcatc
    13021 cttaacagac aacaacttcg taactccttc tttttctatg aatgaaacag ctcgttcaat
    13081 atctggagac atttgttctt catcataaag agcatagcta ttttcaatca cattgctggc
    13141 gaacaactgc atacctacag cagagcccaa agcatccatg tctaaatttt tgtgaccgac
    13201 tacaaaaacc tgatctacac tccgaatctt atctgaaata gctgtcatca tagcgcgcgt
    13261 acgagtccgt gtacgcttga ttgaagcagc agacccacca ccaaaataaa ctggattttt
    13321 cgtttcgtcg ttttccttaa caaccacctg gtcgccacca cgtacttcag ccaagttcaa
    13381 attgagcaaa gcaactttcc ctatctcatc atgatttcca tcgccataag aaaatcccat
    13441 acttaaggtc aagggcaact gtctctgttt cgactcttct ctgaaagcat caataacaga
    13501 aaatttatca ttcatcaagc cctcaagcac cgtgtagtca gtaaatagat aaaatcgatc
    13561 catacttacc cgacgagaaa acatcatgta tttttctgaa aactctgata taaaattagc
    13621 tacaaaacta ttgatttgac taatatctga ctcagaagtt gcatcctcca aatcatcata
    13681 attatccaca gagacaatcc caatcactgg tctacttgtt accaattcat ctgttatggc
    13741 ttgttccctg gatacatcta caaaatacaa aacaccggaa gaagcatcca tatgaacagc
    13801 ataacgcttc tcaccaagct tggcataagt agacggattt cctactgaag ccttgataat
    13861 cgtttgaaca gcttctaaat caaaatcacc atcttccttg gtcaaaatca attcagcata
    13921 gggattaaac cactcaacct ctccagaaga taaattcaat ttcataacac ctacaggcat
    13981 ctgttccaat agagctgtca aactttcttc cgcttggtgg tttacatact gtatctgttc
    14041 tacatcactc cttgtataat gcactctcag tttcttaaat aaaaaaacat agcctcctac
    14101 aaaaagaaac aaaattaaaa ccgtcaacag attattatta acaaaaataa tgaaagtgga
    14161 taagactcca aacgcaatca atgctactag aataggaaaa attggactta cataaaattt
    14221 tttcattcaa aacctcttgg cacccattat accataatac ccctcaaaaa gcgacttttt
    14281 aaaagtgtaa tcagtaattc tatcaattat aagaaaaagg tagtttacaa ttcagtaaac
    14341 ctacctttac acatattgaa attaagattc tttaacctct aacaaaccaa tttcgccatc
    14401 ctcacgacga taaatcacat tggttgtctg atcttcaaca tccacataga taaagaaatc
    14461 atgccccaat aaatccattt gtagaattgc ttcttccaaa tccattggtt ttaaatcaat
    14521 ttgttttgaa cgaacaactt tagactggac aatatttgaa tcttccacca aagcatctgt
    14581 aaataattga ccagttgcta ccttattttt atttttacgc tcgatttttg ttttattttt
    14641 acgaatctga cgttcaattt tatcagttac aaggtcaatt gaaccataca tatcttgaga
    14701 tacatcttct gcgcggagag taatagatcc aagcggaatc gttacttcca ctttagccgt
    14761 tttttcacga taaactttta agttaattcg ggcatccaac tcttgttctg gttggaagta
    14821 cttttcgatc ttttcgagtt tagaaactac ataatcacga attgcttctg ttacttctag
    14881 gttttcacca cggatactat atttaatcat atgagtacct tctttctaaa catttttgtt
    14941 tttatgattt tattataacg ctttcattct atttttgcaa attttttcct catcttacaa
    15001 gggaaaatgt ttttacatcc ttagcaccag cttcttccaa cagtttctta acacgattta
    15061 tagttgctcc tgtagtatag atatcatcta taagtaggat ttttttagga atagtgactc
    15121 cacttttaat aaagaaagga agttctgtcc ccaagcgctc tgaacgattt ttagaagaac
    15181 tggctctctc ttctcttttc tctaataaat ccagatactc aaagcctgct gcctctacca
    15241 agccctcaac ctgattaaat cctctattag catatctatc aggacttagg ggaattacaa
    15301 caaattgata ctctttgtac tttttcaact cctcacttaa aaatgaagcg aaaacttttc
    15361 ttaacaggaa gtctccatca aacttatacc gactgaaaaa atccttcata gcttgattgt
    15421 aagtaaaaat cgctctatga ctgacttcaa ctccttcttt acaccaaagt tgacaatctt
    15481 gacactttgt tgacaactct gttttcatac aatttggaca gttctcttcc ccaattcttt
    15541 caaaagtaga atcacagtct gaacaaagac aagagtcatc attcctcaga agtaagagac
    15601 tactaaaagt taaaacagtc ttcatagtct gcccacataa caagcacttc atagaccagc
    15661 ctccttattc atcatctgaa tttccttaat cgccttcttg attgaagcat ttaacccatc
    15721 atggaagaaa agcaaatctc ctgtcggtct atccatgctt cgtccaactc gtccaccaat
    15781 ctgaatcaaa ctagacttgg taaacaaacg atgattggcc tctactacga aaacatccac
    15841 acaagggaag gtaactccac gctccaagat tgtcgtactg ataagtattg tcagttctcc
    15901 atctcgaaaa gcttgtactt gctctaatcg atcctctgtt acagaagata caaagccaat
    15961 tttctcattt ggaaattgct cctgtaagat ttcttctaac tgctcccctt tcttaatttc
    16021 tgaagcaaaa atgagtaacg gataagctgt ctttctctgc ttctcaatat aggactttaa
    16081 ctttggtgac aaacgattct tgtctaagta gcgattaaaa tccgataacc aaattggttt
    16141 tggaataatc aacggatttc catgaaaccg tctcggtaaa ttcagtcttt ttagttctcc
    16201 taaacggacc tttttatcta actcattggt cgaagtcgct gttaaaaaga ttctcaatcc
    16261 attctccttt acactattct tgacagcatg gtaaagcgtg ggattatcaa cataaggaaa
    16321 agcatctact tcatccacta tcagcaaatc aaaagcttga taaaacttca ataactgatg
    16381 ggttgttgca acaactagtg gtgttcgaaa ataaggttcc gattctccat gtagcaaagc
    16441 tatcccgcaa gaaaaatcct gttgcaggcg cttgtacagc tccaaacaaa catctatgcg
    16501 aggactagcc aaacacactg caccacccgc attgatcact ttagccacta cttgataaat
    16561 catttctgtc tttccagctc ctgttaccgc atgaactaag gttggctttt gcttgtctac
    16621 tgcttgaagc agtccctctg acaccttctc ttgaaaagga gttaattggc cgcgccattt
    16681 gagaacatct tgcttcggaa aatcctcctg cggaaaatag tataaagttt gatcacttct
    16741 gactcgcttc atcagcaagc actctcgaca atagtaagca ccgatgggca aataccattc
    16801 tgctagaata gtgctattac aacgttgaca gaaaagtttc cccttctcct ttctcattgc
    16861 tggaagtttc tccgccaact gacgttcttc ttctgttaat tcattctcag taaataaacg
    16921 accgagataa tctaaattta ctttcatact tctttattcg taaaaactag cactttagat
    16981 gattttttag tacaattaaa tcatggaatt taggacaatt aaagaggacg gccaagtcca
    17041 agaagaaatc aaaaaatctc gctttatctg ccatgccaag cgtgtttata gcgaagaaga
    17101 ggctcgtgac ttcattactg ccatcaaaaa agaacactac aaagcgacac ataactgctc
    17161 tgcctttatt attggagaac gtagtgaaat taaacgtaca agtgatgatg gtgagcctag
    17221 tggtactgct ggtgttccca tgcttggggt actagaaaat cacaatctca ccaatgtctg
    17281 tgtggtcgtg acacgctact ttggtggtat taaactaggc gctggaggac taattcgtgc
    17341 ttacgccggc agtgtcgcct tagctgtcaa agaaattggt attattgaaa taaaagaaca
    17401 ggctggcatt gctattcaaa tgtcttatgc tcagtaccaa gagtacagta acttccttaa
    17461 agaacatgat ctcatggagc tggatacaaa ctttacagat caagtcgata cgatgattta
    17521 tgttgataaa gaagaaaaag aaactattaa agctgcactt gtggagtttt ttaatggaaa
    17581 agtcacttta actgaccaag gtttacgaga ggttgaagtt cctgtaaact tagtgtaaac
    17641 aatgaataat acagcgtttc gttgacattc tcacaactac tttagcgagc aaaataaaaa
    17701 gatgcgtacc aaaatatact agaaaatgaa gcaattcaaa cgaaacctga tatcgttttc
    17761 cttcacacct atttactaga attaactgaa cgcaatcact tgaaaattaa tgactttgat
    17821 ctatgatata tagaaatcct atcgcttcga taggatttct atattttaca aatggtatgg
    17881 atagcgttat actaaagata tcttatacaa agaggtattc atatgtctat ttataacaac
    17941 attactgaat taatcggtca aacaccgatt gttaaactta acaacatcgt gccagaaggt
    18001 gctgcagacg tctatataaa gcttgaagca tttaatcctg gttcatctgt aaaagaccgt
    18061 attgccctta gcatgattga aaaagctgaa caagatggta ttctgaaact tggttctact
    18121 attgttgaag caacaagtgg aaacaccggt attggacttt catgggtggg tgctgctaaa
    18181 gggtataaag tcgtcatcgt tatgcctgaa actatgagtg tagaacgacg taaaattatc
    18241 caagcttatg gtgctgaact cgtcctaact cctggtagcg agggaatgaa aggtgctatt
    18301 gctaaggctc aagaaatcgc tgctgaacgt gatggtttcc ttcctcttca atttgacaat
    18361 ccagctaatc cagaagtaca cgaaagaaca acaggagctg agatactagc tgctttcggt
    18421 aaagatggat tagatgcctt tgttgctgga gtaggtactg gtggaacgat ttctggtgtt
    18481 tctcatgcac tcaaatcaga aaattctaac attcaagttt ttgcagtaga agcagatgaa
    18541 tctgctattc tatctggtga aaaacctggt cctcacaaaa ttcaaggtat ctcagctgga
    18601 tttattcctg atacacttga tactaaagcc tatgatggta tcgttcgtgt aacatcagat
    18661 gacgctcttg cactcggacg tgaaattggt ggaaaagaag gcttccttgt agggatttcc
    18721 tcagctgcag ctatctacgg agccatcgag gttgccaaaa aattaggtac aggtaaaaaa
    18781 gtccttgccc tagcaccaga taacggtgaa cgttatctct ctacagcact ttatgaattg
    18841 taaccgtcca ataacgaagt ctattgaaaa atctccagac tagagaactc acggatagtt
    18901 cctaatctgg agatttctta tttgcacttt tcttgtacaa ctttagtcca tggt
//
LOCUS       DAVMEI010000022        18667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVMEI010000022 DAVMEI010000000
VERSION     DAVMEI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.022"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(163..969)
                     /gene="yidA"
                     /locus_tag="VYQ24_000621"
     CDS             complement(163..969)
                     /gene="yidA"
                     /locus_tag="VYQ24_000621"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1967794.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKSNDESGVAHAIRTWVL"
     gene            complement(982..2286)
                     /locus_tag="VYQ24_000622"
     CDS             complement(982..2286)
                     /locus_tag="VYQ24_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1967795.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            2358..2735
                     /locus_tag="VYQ24_000623"
     CDS             2358..2735
                     /locus_tag="VYQ24_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1967796.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKNSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2826..3158
                     /locus_tag="VYQ24_000624"
     CDS             2826..3158
                     /locus_tag="VYQ24_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1967797.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            3170..4741
                     /gene="ffh"
                     /locus_tag="VYQ24_000625"
     CDS             3170..4741
                     /gene="ffh"
                     /locus_tag="VYQ24_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1967798.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4936..6219)
                     /locus_tag="VYQ24_000626"
     CDS             complement(4936..6219)
                     /locus_tag="VYQ24_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1967799.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSIGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6473..7186)
                     /gene="rsmG"
                     /locus_tag="VYQ24_000627"
     CDS             complement(6473..7186)
                     /gene="rsmG"
                     /locus_tag="VYQ24_000627"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1967800.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7280..7840
                     /locus_tag="VYQ24_000628"
     CDS             7280..7840
                     /locus_tag="VYQ24_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1967801.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7842..8741
                     /gene="htpX"
                     /locus_tag="VYQ24_000629"
     CDS             7842..8741
                     /gene="htpX"
                     /locus_tag="VYQ24_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1967802.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKHM"
     gene            complement(8890..10449)
                     /locus_tag="VYQ24_000630"
     CDS             complement(8890..10449)
                     /locus_tag="VYQ24_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1967803.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQSVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10864..11004)
                     /locus_tag="VYQ24_000631"
     CDS             complement(10864..11004)
                     /locus_tag="VYQ24_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEV1967804.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQVTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            11173..11715
                     /locus_tag="VYQ24_000632"
     CDS             11173..11715
                     /locus_tag="VYQ24_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1967805.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11715..12344
                     /gene="nth"
                     /locus_tag="VYQ24_000633"
     CDS             11715..12344
                     /gene="nth"
                     /locus_tag="VYQ24_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1967806.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12555..13076
                     /gene="pyrR"
                     /locus_tag="VYQ24_000634"
     CDS             12555..13076
                     /gene="pyrR"
                     /locus_tag="VYQ24_000634"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1967807.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13095..14018
                     /locus_tag="VYQ24_000635"
     CDS             13095..14018
                     /locus_tag="VYQ24_000635"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1967808.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14068..15147
                     /gene="carA"
                     /locus_tag="VYQ24_000636"
     CDS             14068..15147
                     /gene="carA"
                     /locus_tag="VYQ24_000636"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1967809.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMALDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15459..18635
                     /gene="carB"
                     /locus_tag="VYQ24_000637"
     CDS             15459..18635
                     /gene="carB"
                     /locus_tag="VYQ24_000637"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1967810.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5444 a         3614 c         4175 g         5434 t
ORIGIN      
        1 tattacaatt cagtaccaca aattaagaaa gcaatgccta ttttttgtac tctcaagatt
       61 taagtaactg tacaactgca attaaattta ttaatttact ctcattgtga tatattcata
      121 caaaaaccgc tcagctgagc ggttttagtc tattctttcc ttttacagta cccatgtacg
      181 gatggcatgg gcaacgccag attcatcatt agatttagtg atgtatttgg cgattttttt
      241 gatttctgga tttccatttt ccatgacaac agggttgcca acgacttcca gcatggcacg
      301 gtcattttct tcgtctccga tagccatggt ttcatctttg gttaatccta gtttttcagc
      361 taagtgagta atagctgaac ccttgtctac attcttttta aggagttcga ggtagaaagg
      421 agcagatttg ttgatagagt agtgctcgta aaattcggct ggtatttttt caatcgcagc
      481 atcgagaatt tctggttcat cgataaacat acatttaaca atttctttgc cagccatttc
      541 ttcaggggta cggtagaaga taggcatgct gacgagggtt gattcgtgta cagtgtatgt
      601 tccgatattg cgatttgcag tatagatacc gtccttggta atggcatgca tgtggacacc
      661 gagcttgcga ctgaggaatt ccatatccag ataatcctca taggtcaagg attcgctgat
      721 aatctcatgg ccagtagcag tttcttggac aagggcacca ttgaaggtca ccacatagtc
      781 accttggtct ctcaactgca agtcgtctag aagtttggca acacctgcga tggggcggcc
      841 agttgcaatc acgactttga caccagcttc tttggcatct tggatggcag aaaagacttc
      901 aggagtgatt tccttttggc tgttgacaag ggttccgtcg atatcaacgg caattagttt
      961 aatactcata aatttcctct tctagttttt atttggggta aaatgatcgt tctcaatcaa
     1021 gtataaaaat tgctgggtaa tgctagcaaa gatactgttt tgatctaaca tttctttcgg
     1081 aaaatagaaa cgattatctc cgtgacgact accagcaagg gattggacga taggagacag
     1141 gctagagagt tcggccagtt ctccattctt ttgtaaaatc tcaatctgtg tccgtggatt
     1201 ttcagattcg ggacgataga tatcataagg gaggtcaaag ttcttatgaa tggcagtata
     1261 gtagtcggga tcaaagccaa tgtcctcaac caattttctc atgctagcga gttggtcttg
     1321 atcttcttgt gaaaaggtaa tggatttaaa gaccttgcgg ttgacaaagc gttgcgataa
     1381 atctgcgaga attttgtcag gactggtcat ccagagctgg aagtaggtat tcatcacacc
     1441 atcatctaga gccagatagt cagacaaggt cacatttttt tcaaagaaag gcaggaggtg
     1501 tggggaagtt cgtgcaaaga agtctttgtc cttagggtag agttccttag cgcgcttgag
     1561 aagattctgt aggagaactt ccatagcgcg tgttgctggg tggaaataga cctgcatata
     1621 catctggtag cgactgagga catagtcttc gatggcgtgc atgccattgc gctgaaaggc
     1681 gataccattt tcgataggac gaatgactcg gaggattcga gtcaggtcaa attccccata
     1741 ggatgctcct gtaaaatagg agtcacgcaa gagatagtcc atgcgatctg cgtcaatctg
     1801 actagaaatg agttgtacga cctgcttgtt aggataggta tggtctatga cactggcaac
     1861 tttctctgga aaatcgggcg ctacttgtag caagacttgg tgaatctctg tctcaggact
     1921 ttgaataatc tcctgagtaa tagcttcatg gtctgtatca aagagatgtt caaaagtatg
     1981 ggagtaggcg ccatgcccaa ggtcatgtag gagagcagcg atcatggtca agagagactc
     2041 ggcaggattc cattcctcag gatatttttc ttcgaaaatc tctgtgatgc gtcgtgcaat
     2101 ttcatagact cctagacagt gagagaagcg actgtgttct ccaccgtgga aggtataact
     2161 ggaagttccc agttgtttga tccggcgcaa acgctgaaat tcttttgtat taatcaagtc
     2221 atagatgatt tgattattga catggatgta gttgtgaact gggtcacgga atactttttc
     2281 gttcatatga ccattatagc aaaatcctgc tctttttggt aaaatagtag gacaagagac
     2341 aagaaagagg gcctgatgtg aagattcgga tgcgaaatac aattcagttt gatgagcagt
     2401 tggaagtgat tgaccagctt tatgatgtgg aagtgcatga aaaaggagat tatagctacc
     2461 tgcttttcta taatgaggaa aaggaaaaag tagtgattaa atttcatggt caagaacttg
     2521 tgatgagccg attttctaat cccaagacca ttatgcgctt tctaaagaat agcgatagtt
     2581 tagcctatat tcctacacct atgggcatgc aggagtttat catccaaacg agccattatc
     2641 aagttgatag gcaaaagatt gaactagatt atcaactaca aaatcaagag ggacatcctt
     2701 ttgccagcta tcaattggaa attacttggg gctaggctca tgtctttttc aaatttagct
     2761 atctatcttc ttggatcggc ttcctagtgt ggtagattag gctaattttt ggtataataa
     2821 aagttatgga aatcgaaaaa accaatcgta tgaatgcgct ctttgaattt tatgcggcgc
     2881 ttttgacaga taagcaaatg aattatatcg agctctacta cgctgatgat tacagccttg
     2941 ctgaaattgc cgaggagttt ggtgtcagtc gtcaggctgt ctatgacaat atcaagcgaa
     3001 cagaaaagat tctggaagat tatgagatga aattgcacat gtactcggac tatattgtcc
     3061 gcagtcagat ttttgatcag attttggagc gctatcccaa ggataacttt ctgcaggagc
     3121 agatagaaat tttaacaagc attgataata gagaataaga ggaagaaaaa tggcatttga
     3181 aagtttaaca gaacgtttgc agaacgtctt taaaaatcta cgtaaaaaag gaaaaatctc
     3241 tgaatctgat gtccaagagg caaccaaaga aattcgcttg gccttgctcg aggccgacgt
     3301 tgccttgcct gttgtaaagg actttatcaa gaaagttcgt gagcgtgcag tcgggcatga
     3361 ggtcattgat acacttaatc ctgcgcaaca gattattaaa atcgttgatg aggaactgac
     3421 agccgtttta ggttctgata cggcagaaat tatcaagtca cctaagattc caaccatcat
     3481 catgatggtt ggtttacaag gggctggtaa aacaaccttt gctggtaaat tggccaacaa
     3541 actcaagaaa gaagaaaatg ctcgtccttt gatgattgcg gcggatattt atcgtccagc
     3601 tgccattgac cagcttaaga ccttgggaca acagattgat gtgcctgtct ttgcacttgg
     3661 aacagaagta ccagctgttg agattgtacg tcaaggtttg gagcaagccc aaactaatca
     3721 taacgactat gtcttgattg atactgcggg tcgtttgcag attgatgagc tcctcatgaa
     3781 tgagcttcgt gatgtgaaag cattggctca accaaatgaa atcttgcttg tcgttgatgc
     3841 tatgattggt caggaagcag ccaatgttgc gcgtgagttt aatgctcagt tggaagtgac
     3901 tggggtcatc cttaccaaga ttgatggcga tactcgtggt ggtgctgctc tgtctgttcg
     3961 tcacattact ggaaaaccaa tcaagttcac tggtacaggt gaaaagatta cggacattga
     4021 aaccttccac ccagaccgca tgtctagccg tatccttggt atgggggata tgctcacttt
     4081 gattgagaaa gcttctcagg aatacgatga acaaaaagcc cttgaaatgg ctgagaagat
     4141 gcgcgaaaac acctttgatt ttaatgattt catcgatcaa ttagatcagg tgcaaaatat
     4201 ggggccgatg gaagacttgc tcaagatgat tccaggtatg gccaacaatc cagcccttca
     4261 aaacatgaag gtggatgaac gccagattgc tcgtaaacgt gccattgtgt cttcgatgac
     4321 acctgaagag cgtgaaaacc cagatttgtt aaatccaagc cgtcgccgtc gtattgctgc
     4381 tggttctgga aatacattcg tcgaagtcaa taaattcatc aaggacttta accaggctaa
     4441 acagctcatg cagggtgtta tgtctgggga tatgaataaa atgatgaagc aaatggggat
     4501 taatccaaat aaccttccta aaaatatgcc aaatatggga ggaatggata tgtctgccct
     4561 tgaaggaatg atgggacaag gcggtatgcc tgacttatca gctctcggag gagcaggaat
     4621 gccagatatg agccagatgt ttggtggcgg tttgaaaggt aaaattggtg aatttgccat
     4681 gaaacagtcc atgaaacgta tggctaacaa aatgaagaaa gcgaagaaga aacgcaagta
     4741 agacaagaga ttgatgcaaa tctcatttga tgagaaaata aagtaaaaat ttctatgata
     4801 aaacgtacag tatcaaggtt ttagaatatc tgatattata cgttttttga ttttaataat
     4861 tttgttaaga cacttttatt atggtatgcg aaaaatccac tcagctgtct gagtggatta
     4921 ggtgtattta gactcttagt ctttattttc gtatggcaag atcaagttca agatgattcc
     4981 tgtcatggct gaaagggcag tacctgaaag tgtaactgga ccaagtttaa ggatagctcc
     5041 tccaagtcca agaaccaaca tagcacttgc gatgatgagg tttcgcattt gagcgaaatc
     5101 aacacgttct ttaatcaaga ctttcaaacc attgctggcg ataaccccat agagaaggat
     5161 tgacatacca ccaagtacag cgtttggaat agttgaaatc aaggcagtga atttaccaag
     5221 gaagctgagg gcaatcgcga tgaaggcagc gttacggata actgagacag aagcgatacg
     5281 agtcataccg ataacccctg tattttctcc gtaagttgta ttggctggtc caccaaggaa
     5341 ggcagaaaca gaagttgcga taccgtcacc aataagagta cggtgaagac ctggttcttt
     5401 taagaattga cggccacaga tttgacccaa aacagtatgg tctccgatat gttcagaaat
     5461 tgttacgata gcgattggca agatagcgat ggcttctgga ccaaagtaaa gattgtactc
     5521 tttaaaggca ccacctatgc taaatggcaa gtagaaacca ggaatttcaa accagttagc
     5581 ttctaggaca ggcgtaaagt caaccaaacc aagagttagt gcgaagaggt aaccaccgat
     5641 aatggcaaag aggaatggaa tgattcgtag gaagcctttt ccttttgtat tgataaaggc
     5701 agcaattagg aaagtaacaa cggctaccag agcatttttc caatttccgt ctgctacaag
     5761 acctgcattg gtaacagctg aacctgcaag tccaagaccg ataacgatga tcataggacc
     5821 gataatgatt ggtggcaaga gtttatcaat ccattttgtt cctacaaatc ggatgctggt
     5881 agcaacaagg acatagacca aaccagtcaa gataacccct gtttgagcag cagatacatc
     5941 cccccccatt tctttcatag ccagtgacat agctgtgata aaggcaaatg aagaacctag
     6001 ataaactgga actttaaaac cagtagcaat catgtagatg agtgttccaa cacctgaagc
     6061 aaaaagggca acagatacag gcattcccaa aatcaatggt accaagatgg tcgcaccaaa
     6121 catggcgaaa acgtgttgga aactaaggag aattcctttt ccatccgaag gacgttggtc
     6181 aacgtctagt aacaaatcaa cagttgattc ttgtttcata taaacctcac ttttgggcat
     6241 cagaaaagag cccattattt tacagcaata ggccctcaga gtaagacttc tctaaaatct
     6301 agactttaaa gagtttcaaa tatttctgcg tcttcgtcac ctcacgggat gacattaaaa
     6361 acctttgctt aggatagtat agcagaaaaa aatgattttg taaatcattt tttcccgagc
     6421 ctaaaaataa gagagcgagg ttgattttgt aaatattttt ttacttaaaa atttaaagcg
     6481 ggcgtttatt tggcatacca gccttacgtg gatatttatt tggtgtttct tttttctttt
     6541 ctaccactgt gatgtaacgc ggatctctat ttggtagggc gtagctgaga ttgtcttcga
     6601 ctttactaaa aaggagattg agggcattct tagcttctaa caactcctca ggcgcattgc
     6661 tagccttgag tgccaatagt ttgccaccaa ccttaaggta gggaatagtc aattcagata
     6721 ggacctgcat acgggcaacc gcacgagctg ttacaaaatc atattgagca cggaagttct
     6781 tgtcttgggc aaaatcttcg gcacgtccgt ggtagaaatg aactccgttc aaatccagtt
     6841 cttgagccaa gagttgtagg aagttgatgc gcttattgag tgaatcaata atggtcacat
     6901 ctaactccgg atagagaatt ttcattggta gactaggaaa tcctgcccca gccccgatat
     6961 caagaagttt gatagtttca ttgggaatca aaccttgaag aatgggtgca atcgaatcgt
     7021 aaaaatgttt gagataaact tcttccttgt ccgtaatcgc cgtcaaatta atcttctcat
     7081 tccactcgac caagagctca aaataacgtt caaattgttc tttttgctgg tccgaaagtg
     7141 gaagattctg ctcggcaagc aagttgtaaa atgtttttgg tttcatagtt atttccttct
     7201 ttagtatcat cttattttac cacaatcata gaaaatttga tatactggta ctaatctaaa
     7261 agcagaaagg acttatatta tgacttggat tattcttgga gttttagctc ttgttgttat
     7321 ttttgtgatt gttagctata acggtttggt taaaaatcgt atgcaaacca aggaggcttg
     7381 gagtcagatt gatgttcagt tgaaacgtcg caatgatctc ttgccaaact tgattgagac
     7441 tgtaaaaggt tatgccaaat atgaaggttc tacccttgaa aaggtggcag aactacgtaa
     7501 ccaagtggcg gcagcgactt caccagcaga agctatgaaa gccagtgatg ccctcactcg
     7561 tcaggtttca ggtatttttg cagttgcaga aagctatcca gatttgaaag ctagtgctaa
     7621 ctttgttaaa ttgcaagagg agttgacaaa cacagaaaat aaaatttctt actctcgtca
     7681 actctataac agtgttgtca gcaactacaa tgtaaaatta gaaactttcc cgagcaatat
     7741 tatcgctgga atgtttggat ttaaagcggc agatttcctt caaacacctg aagaggaaaa
     7801 gtcggttcct aaagttgatt ttagcggttt aggtgactaa gatgttgttt gatcaaattg
     7861 caagcaataa acgaaaaacc tggattttgt tgctggtatt tttcctactc ttagctcttg
     7921 ttggttatgc ggttggttat ctctttataa gatctggact tggtggtttg gttatcgcac
     7981 tgattatcgg ctttatctac gctttgtcta tgatttttca atcgacagag attgtcatgt
     8041 ccatgaatgg agcgcgtgag gtggatgagc aaacggcacc agacctctac catgtagtgg
     8101 aagatatggc tctggtcgct cagattccta tgccccgtgt tttcatcatt gatgatccag
     8161 ccttaaatgc ctttgcgaca ggttctaatc ctcaaaatgc ggctgttgct gcgacttcag
     8221 gtctactagc tatcatgaat cgtgaagaac tagaagctgt tatgggacat gaagtcagtc
     8281 atattcgtaa ttatgatatc cgtatttcga ctattgcagt tgcccttgct agtgctatca
     8341 ccatgctttc tagtatggca ggtcgtatga tgtggtgggg tggagcaggt cgcagacgaa
     8401 gtgatgatga ccgagatgga aatggtcttg aaatcattat gctagtggtt tccctactag
     8461 ctattgtact ggcacctctc gctgcaacct tggttcagct cgctatttct cgtcagaggg
     8521 aatttctggc agatgcatct agtgtcgagc tgactcgcaa tccccaggga atgattaatg
     8581 ccctagataa gttggacaat agcaaaccta tgagtcgcca cgtcgatgat gctagcagtg
     8641 ccctttatat caatgatccc aagaaaggtg gggggttcca aaaactcttt tatacccacc
     8701 cacctatctc agaacggatt gaacgtttaa aacatatgta aaatgaaggc tggaaaaaag
     8761 tctttaaaat ctgaaaaacg cataatatca ggtgtgaaaa cttgatatta tgcgttttac
     8821 tatgggaaga tttacttctt tttctcctaa aattgtgttt ttgccccacc tatctgctat
     8881 gttgcaaatt tataaatctt ctaaattaac tagcttcaaa ccgtgttctg tcatgcgata
     8941 gatgatcgag cagacttctt ttaagaaacg acggtcatgc gaaacagtga tgagaccgcc
     9001 aggataagtg gcaaagagtt ttctgatttg gggttgagaa gtgggagaaa agtttcgtgt
     9061 gggttcatcc agcaggagaa agtttggttt gcgcaggact aaatccaaaa gcaggagttt
     9121 tccctgttgt ccgccagata aggagcgaat ttgatgctgc atttctggat aactgaaatt
     9181 gagactagct aggtgagatt ggattttctg tagttcctct ttttccccag ttttactgag
     9241 ataggctatt ggggataaat ccaattgcag ttttttgtgg taatcttgtg gcataaaacc
     9301 aagtgaaatc tctcttttat cattcagaag tctctgtaac ttggctaaca gagttgattt
     9361 cccaacacca tttggcccga taataccgat tttttcttgg ccacggacag ttagttgtag
     9421 tttttgaacc aaaactcggt cgtcaatgga caaattttct ttttccagtt ggactaagac
     9481 tttagaagct ggtaatggtt ggatgtctga aaagaaaagt tggatttgtt cctcttcaag
     9541 tggcttttga gtcatggact gagctgcctt ttcgtagcgt ttttcttgtg agaggacagt
     9601 tttcatcttt ttagccaata ggcgaccggc agtactatct ttggtagctc gaagcgcagt
     9661 ttctacactt tgcttgactc tccgatgttt ttccatggtt ttatcgtagg ctctttggtt
     9721 gttagcagct tgctgacttt gtttggcaaa attagccttt ctctgctcac tatagctatc
     9781 atagtctaaa tgctctacta gcgtttccgc ttctttacgg tgtttgacca gtcgcaagtg
     9841 aacaatagtg tctgccgttt cagaaagaaa gtcttcatca tgggaaatga aaataacggt
     9901 ttgcctggtc ttttgaatct ggccttttag ccaatcaact gtctcaaggt ctaggtcatt
     9961 tgaaggttca tctaaaaata gaatctcaaa gggtttggct aactcatgga taagctgaat
    10021 tttcaaagct tcgccccctg atagattgcc aatttcttgg tcacttgcga aacgattgct
    10081 atcaaaatgc aattcctccg ccaaacgata gaggatactg tagtctaaat caatagaatc
    10141 taaaaagaag tagtcgtgta aagttttctt ttttaggtcc tcagggactt tttgaggaat
    10201 gtaggccagt gactgataat cagactggat gtctcccttg atagtgaaat cagacaaatc
    10261 ttcccccatt aaagttttaa gtaaggttga tttaccattt ccttcttcgc caataatagc
    10321 taccttttcc ccgtcttgta tggtcatgct taggtcagag acaaggtctc gtaaatcttt
    10381 gttttgtgtg atggtcaggt gattgatttt tatcatacta ttgccctttc tagaatgtcg
    10441 atagtgcata acaaaaagct ctactttacc tagtagagcc caaagtctat gtcaaaaaaa
    10501 ctctattaac atgccagaaa aagagcataa aagagcacac aaaaagagac aaaaacaaga
    10561 tctactaaat aaaggttctt tatttgatag acttagttgt tcatgtctcc cttacctccg
    10621 agtattagta tctttatatt atacctttaa ggaaattttg tcaacagaaa actggaaatt
    10681 ctatgctcta aaatccaaaa acaggtccat agagacttag tacgtgtttg acatgctcgt
    10741 aatggatttt atcatagttt cgccaagcag tgcgtgcttg attgtttagt tttaggctac
    10801 ccagtccaat cagaggactg gtgggttggt aaaatttctc aagattaatt aagatactgt
    10861 tgttcaactt ttctgttttt tgagctcttg catagtagtg tttagcagct cttgtagtcg
    10921 aatatcatct gccgtaacct gtttacagtt ttggtagctt ttatttaact tggataggag
    10981 ttgaatgagt ttcttgtctt ccatgtgagc atcctccttg atctgatgta ctctttccct
    11041 atagtatagc actaaaaaat aaaactatca tagtaaatat gttaaaattt gaattttaat
    11101 tacaaagttt ggtgtgctga tttgtagcct tttcatggta taatcaagag agtaaatctt
    11161 tatggaggaa gtatgaaatt aaatattcaa gaaattcgta agcagtctga aggtttgaac
    11221 tttgaacaaa cgttagattt agttgatgac ctgcgtgcac gtaatcaaga aattttagat
    11281 gtaaaagata tccttgcagt tgggaaagta caatatgaag accgtatgta tttcttagat
    11341 tatcaactat cttataccat tgttcttgct tcgagtcgca gtatggagcc agttgagtta
    11401 gttgaatctt atccagtcac ggaagttttc atggaaggcg caactaacca gctagatcaa
    11461 gaagttttag atgatgactt ggtcttgccc atcgaaaatg gggcgcttga ccttgctgag
    11521 agtgtatcag acaatatcct gctaaacatt cctatcaagg tcttgacggc tgaagaagaa
    11581 gctggtcaag gatttgtctc aggaaatgac tggcaaatca tgacagagga agaataccaa
    11641 gctcaacaag cagtaaagaa agaagaaaac agtccttttg ctggcttaca aggactattt
    11701 gacggagatg aataatggtc ttgtcaaaaa aacgagcacg aaaggtgcta gaagaaatca
    11761 ttgccctctt cccagatgcc aagcctagtc ttgattttac caatcatttt gaactcctgg
    11821 ttgcggtcat gttatcagcc cagacgacag atgcagcggt aaataaggcc acaccaggtc
    11881 tctttgttgc ctttccaaca ccccaagcta tgtctgtagc gacagagagt gagattgctt
    11941 cacatatttc tcgcttggga ttgtatcgga ataaagctaa attccttaaa aaatgtgccc
    12001 aacagttact agacgatttt gatggtcaag tccctcagac acgtgaagaa ttggagagtt
    12061 tggcaggtgt tggtcgcaag acagccaatg ttgtcatgag tgtaggattt gggattccag
    12121 cctttgcagt ggatactcat gtggagcgta tttgcaaaca ccacgatatt gtcaaaaaat
    12181 cagcgacgcc acttgaggtg gaaaagcggg tcatggatat cttgccgcct gagcagtggt
    12241 tagctgccca tcaggccatg atttattttg gaagagccat ttgccatcca aaaaatccag
    12301 agtgtgacca gtacccacaa ttatatgatt ttagcaattt gtaagatgct acattgaaac
    12361 tttttgcttg attttaagca tgctttgtgc tatagtaaat gataactaaa tattccttta
    12421 aacctgtccc gtgaggcagg caaggagcgc gataaagtag aagggtgaag tctacactgt
    12481 gtgcagtagg gctcgcttgg ctataaattt caagtctcct tgtttaagga gacttttctt
    12541 tttggaggtt tgtcatgaaa acaaaagaag ttgtagacga attgaccgtc aaacgagcga
    12601 ttacgcgtat tacttatgag attatcgaac gcaacaaaga tttgaataag atcgtcttgg
    12661 ctggtattaa aactcgtggt gtctttattg cccaccgaat ccaagaacgt ttgaagcagt
    12721 tagaaaatct ttcagttcct gttgtggaat tggatactaa acctttccgt gatgatgtta
    12781 aaagtggaga agatacttct ttggtttctg tcgatgtgac agaccgcgaa gttatcttgg
    12841 tggatgatgt gctctataca ggtcgtacca tccgtgctgc tattgataat attgtcggtc
    12901 atggtcgtcc tgcgcgtgtg agtttagcag ttctagtcga tcgtggacat agagaattgc
    12961 caatccgtcc agattacgtt ggaaaaaata tcccaaccag tcgttctgaa gaaatcatcg
    13021 tagagatgac agaacttgat gaccaagaca gagttctgat tactgaagaa gcttagaaag
    13081 ttaaaggagt agccatgtca gaaaatcaac aagcattgaa ccatgtggtg tccatggaag
    13141 acctcactgt cgatcaagta atgaaattga tcaagcgagg aattgagttt aaaaatggag
    13201 cccagcttcc ctatgaagac catccgattg tttccaatct cttctttgag gattctacac
    13261 ggacacataa gtcctttgaa gtcgcagaga ttaaacttgg attggaacga cttgactttg
    13321 atgtgaagac tagttcggtt aataagggtg agacacttta tgacaccatt ttgactctgt
    13381 ctgctttagg agtggatgtc tgtgtgattc gccatccaga ggtcgactac tatagagaat
    13441 tgattgctag tccaacgatt acgacttcca tcatcaatgg tggagatggt tcgggccaac
    13501 accctagcca gagcttgctt gatttgatga ccatttatga ggaatttggc cactttgagg
    13561 gtcttaaagt tgctattgca ggtgacttgg atcactcacg cgttgccaaa tccaatatgc
    13621 agattttgaa acgcttggga gctgaactct ttttcgctgg acctgaggaa tggagaagtc
    13681 aagagtttgc agactatgga cagtttgtaa ccattgatga aatcattgat caggttgatg
    13741 tcatgatgtt tctccgtgtg caacacgaac gccatgatag tggagctgta ttttcaaaag
    13801 aagactacca tgcccaacat ggcttgactc aagaacgtta cgatcgtttg aaagaaacag
    13861 caatcctcat gcacccagct ccaatcaatc gtgatgtaga aatagcagac cacttggttg
    13921 aagcaccaaa atcacggatt gtccaacaaa tgaccaatgg tgtctttgtt cgaatggcaa
    13981 tcttagaatc cgtactagcg agtagaaacg ccaactaaaa cacaagacgt taaaagacgc
    14041 cagtgagcgt ccacgagagg tgaaaatatg acaaaaagaa ttctagtatt agaagatggc
    14101 acagtttttg aaggcaaggc cttcggagca gatattgatg taacaggcga aatcgtcttt
    14161 aatacaggga tgaccggcta ccaagaatcc attacagacc agtcttataa tggacaaatc
    14221 ttgaccttta cttatccttt ggtaggaaat tatggtatta atcgtgatga ttatgaatcc
    14281 attattccaa cttgtaaggg agtcgttgtt tttgaagaag cgcgtagagc tagcaactgg
    14341 cgcaaccaaa tggccttgga tgaatttttg aaagccaaga aaattccagg gatttcagga
    14401 attgatacgc gtgctcttac caagattatc cgtaagcatg gtactatgcg tgcaaccttg
    14461 acccatgttg gggacagtat ggaccatgtg acggaccagc tccaagcaac agttttaccg
    14521 acagacaata tcaaacaggt ttctactaaa acgtcttatc cagctccagg agttggtttg
    14581 agcgtggtgc tagtggactt tggtctcaag cactcaatcc tacgtgaact ttctaaacgc
    14641 aactgtaacg tgacagttgt tccttattcg acaacggctg aagaaattct ccatctcaat
    14701 cctgatggag ttatgttgtc aaatggtcca ggtaacccag aagacgttcc acaagcactc
    14761 gacatgattc gcggtgtaca aggaaaaatt ccaatctttg gtatttgtat gggacaccaa
    14821 ctctttgcaa tggcaaacgg ggctaagacc tataagatga agtttggcca tcgtggattc
    14881 aaccatgcgg tacgtgaaat cgcaacagga cgagtagact tcaccagcca gaaccatggt
    14941 tatgcagtca gccgcgagga tttgccagag cacttgatta ttacccacga agaaatcaat
    15001 gacaagtcag tggaaggtgt gcgccacaga taccaacctg ctttctctgt tcaataccac
    15061 ccagatgcag ctcctgggcc acacgacgct agctacctat ttgacgagtt tatcgagatg
    15121 atggaagttt ttaaacaatc aaactaagaa cgagtaaaag ctcgtcggag aatttatgta
    15181 actgaacacg gacggaaagc tcagaattta gagaaaccaa cttggattgt caatccttcc
    15241 ttgatttctt aaaattcaat cgctttctat tcgtcctttg tatcttattt aatgtcgccc
    15301 tgagaggcga cgaatagaaa ggagaagata cattgttcgc ggaagtgaac acgccctaag
    15361 aactgtgtga aaaagataaa catcgtcttg acgctgaagg cgtctgcgcc gagtttccta
    15421 ttttcactgt gttctttacg ggctttgtat cataaactat gcctaaacgt actgatattc
    15481 aaaaaattat ggtgattggt tctggtccga ttattattgg tcaggctgct gagtttgact
    15541 acgctgggac ccaggcttgc ttgtcgttga aagaggaagg ttatgaggtt gttttggtta
    15601 actcaaatcc tgcaaccatc atgacggaca aggaaattgc tgacaaggtt tacattgaac
    15661 cgattacact tgagtttgtg acacgtattc ttcgtaagga acgtccagat gccttgctac
    15721 caacactcgg tggtcagaca ggtctcaaca tggccatgga attatctaaa aatggtatcc
    15781 tagacgagct tggcgttgaa cttctgggta ccaaattatc tgccattgac caagcggagg
    15841 accgtgacct ctttaaacaa ttgatggaag agcttgagca acccattcca gaatctgaaa
    15901 ttgttaacac agtagaagaa gcagttgcct ttgcagcgac aattggctac ccagtcatcg
    15961 ttcgtccagc ctttaccctt ggtggtactg gtggtggtat gtgtgccaac gaaaaagaat
    16021 tgcgtgaaat cactgaaaat ggtttgaaat tgtcacctgt tacccaatgt ttgattgagc
    16081 gttcgattgc cggttttaag gaaattgaat acgaagtgat gcgtgactca gctgacaatg
    16141 ccttggttgt ttgtaacatg gaaaactttg acccagttgg gattcacaca ggggattcca
    16201 ttgtatttgc ccctgcgcaa accatgtcag actatgaaaa ccaaatgctt cgtgatgcga
    16261 gcttgagcat tattcgcgcc ctcaagattg aaggtggatg taatgttcag ctagctcttg
    16321 atcctaacag cttcaagtat tatgttatcg aagtaaaccc tcgtgtatca cgttcgtcag
    16381 cccttgcttc taaagcgaca ggatatccga ttgctaagtt ggctgccaag attgcagtag
    16441 gtttgacctt ggatgaggtt ataaacccag ttacaggttc aacctatgcc atgtttgaac
    16501 cagcccttga ctacgtagtt gctaagattc cacgtttccc atttgacaag tttgaaaagg
    16561 gtgaacgccg tctcggtaca caaatgaagg cgactggaga agtcatggca atcggtcgta
    16621 acatcgagga atctcttctc aaggcctgcc gctcccttga aattggggtg caccacaatg
    16681 aaatacctga acttgcagca gtttctgatg atgccttgat tgaaaaggtt gtcaaagccc
    16741 aagatgaccg tctcttctac gtatcagaag ccattcgtcg tggctacaca cctgaagaaa
    16801 ttgcagagct tacaaaaatt gatatcttct atcttgataa actcttgcat atctttgaaa
    16861 ttgagcaaga attgggtgct catccacaag atctagaagt tttgaaaaca gccaaactta
    16921 atggtttctc agaccgtaag attgctgaac tctggggaac gactgatgat caagttcgcc
    16981 aacttcgctt ggaaaacaag attgttccag tttataagat ggtagatact tgtgcggcag
    17041 agttcgactc tgaaacgcca tatttctatt caacctatgg ttgggaaaat gaatctatca
    17101 gatctgataa agaatctgta cttgtcctag gttcaggtcc aatccgtatc ggtcaagggg
    17161 ttgagtttga ctacgcaacc gttcactctg ttaaggctat ccaggcagct ggttatgaag
    17221 ctatcatcat gaactcaaac ccagagaccg tttcaacaga cttctctgta tcagataagc
    17281 tttactttga gccattgaca ttcgaagatg ttatgaacgt cattgacttg gaacaaccaa
    17341 agggagttat cgttcagttt ggtggtcaaa cagccatcaa ccttgcggag ccattggcaa
    17401 aagcaggtgt gaccatcctt ggtacacagg ttgctgacct agaccgtgcc gaagaccgtg
    17461 acctcttcga gcaagctctc aaagaattgg atattccaca gccaccagga caaacggcta
    17521 ccaatgaaga agaagcagct cttgcagctc gtaagattgg cttcccagtt cttgtccgcc
    17581 catcttatgt acttggtgga cgtgccatgg aaatcgttga aaacgaagaa gaccttcgtt
    17641 cttacatgcg aactgctgtt aaggctagtc cagatcaccc agttcttgtc gactcttata
    17701 tcgttgggca agagtgcgaa gttgatgcca tttcagatgg taaagatgtg ctcattcctg
    17761 gtatcatgga gcatatcgaa cgtgctggtg tccactcagg tgactcaatg gctgtttacc
    17821 caccacaaac cttgtcgcaa aaggttcagg agaccatcgc agactacacc aaacgcttag
    17881 caatcggtct tcactgcctt ggaatgatga acatccagtt tgttatcaag gatgaaaaag
    17941 tctacgttat tgaggtcaat ccacgtgcca gccgtactgt tccattccta tctaaggtaa
    18001 ccaatattcc tatggctcaa gtagcgacca agctcattct tggtcaaagt ttatcagaac
    18061 ttggctacca aaatggactt taccctgaaa gcacccgtgt tcatatcaag gcacctgtct
    18121 tctcctttac caaactagct aaggtagaca gcttgctcgg tcctgaaatg aagtcaacag
    18181 gtgaagttat gggttctgat gctactttgg aaaaagctct ctataaagcc tttgaagctt
    18241 cttatctaca cttgccaact tttggtaatg tcgtctttac catcgctgat gatgccaaag
    18301 aagaagcctt gaacttggct cgtcgtttcc aaaacattgg ttatggaatc cttgcgacag
    18361 aagggacagc agccttcttt gctagtcatg gcttgcaagc ccaacctgtt ggtaagattg
    18421 gtgatgacga taaggatatc ccaagttttg tacgcaaagg aagaattcaa gctatcatta
    18481 acacagtagg aacaaaacga actgctgacg aagatggtga gcaaattcgt cgttcagcca
    18541 ttgaacacgg ggtgcccctc ttcacagcgc tagatacagc taatgccatg cttaaggtac
    18601 ttgaaagtcg tagctttgtt acagaagcga tttaaataga aaaataagat atagcagact
    18661 gaattta
//
LOCUS       DAVMEI010000023        18423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVMEI010000023 DAVMEI010000000
VERSION     DAVMEI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.023"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..1586
                     /locus_tag="VYQ24_000638"
     CDS             105..1586
                     /locus_tag="VYQ24_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1967811.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRITSQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1947..2948
                     /locus_tag="VYQ24_000639"
     CDS             1947..2948
                     /locus_tag="VYQ24_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1967812.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILEKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3098..4015
                     /locus_tag="VYQ24_000640"
     CDS             3098..4015
                     /locus_tag="VYQ24_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1967813.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4026..4916
                     /locus_tag="VYQ24_000641"
     CDS             4026..4916
                     /locus_tag="VYQ24_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1967814.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4945..6561
                     /locus_tag="VYQ24_000642"
     CDS             4945..6561
                     /locus_tag="VYQ24_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1967815.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6571..7890
                     /locus_tag="VYQ24_000643"
     CDS             6571..7890
                     /locus_tag="VYQ24_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1967816.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVVTGTDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8101..8173)
                     /locus_tag="VYQ24_000644"
     tRNA            complement(8101..8173)
                     /locus_tag="VYQ24_000644"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8138..8140),aa:Lys,seq:ctt)
     gene            complement(8438..9709)
                     /locus_tag="VYQ24_000645"
     CDS             complement(8438..9709)
                     /locus_tag="VYQ24_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1967817.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            10007..10222
                     /locus_tag="VYQ24_000646"
     CDS             10007..10222
                     /locus_tag="VYQ24_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967818.1"
                     /translation="MMELPSQEILIFTKQIRHWILSDQVISGKRKLFFREDTPKEILD
                     LYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10212..10460
                     /locus_tag="VYQ24_000647"
     CDS             10212..10460
                     /locus_tag="VYQ24_000647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967819.1"
                     /translation="MKNRIIDVFEVVNRLLVITVENPDFEDLRVNQFVKIGDKKYRVR
                     SVPMIHSTPPQSVLDRDTFTIDYTDDELLDKEAVFTTH"
     gene            10597..10776
                     /locus_tag="VYQ24_000648"
     CDS             10597..10776
                     /locus_tag="VYQ24_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1967820.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            10813..11265
                     /locus_tag="VYQ24_000649"
     CDS             10813..11265
                     /locus_tag="VYQ24_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1967821.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11556..12026
                     /locus_tag="VYQ24_000650"
     CDS             11556..12026
                     /locus_tag="VYQ24_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1967822.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12045..13133
                     /locus_tag="VYQ24_000651"
     CDS             12045..13133
                     /locus_tag="VYQ24_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1967823.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLMLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13114..13542
                     /locus_tag="VYQ24_000652"
     CDS             13114..13542
                     /locus_tag="VYQ24_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1967824.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            13539..14012
                     /locus_tag="VYQ24_000653"
     CDS             13539..14012
                     /locus_tag="VYQ24_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967825.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            14150..15100
                     /locus_tag="VYQ24_000654"
     CDS             14150..15100
                     /locus_tag="VYQ24_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1967826.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPETVDVAAVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            15102..15845
                     /locus_tag="VYQ24_000655"
     CDS             15102..15845
                     /locus_tag="VYQ24_000655"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1967827.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVSGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15856..17652
                     /gene="pepF"
                     /locus_tag="VYQ24_000656"
     CDS             15856..17652
                     /gene="pepF"
                     /locus_tag="VYQ24_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1967828.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(17712..18389)
                     /locus_tag="VYQ24_000657"
     CDS             complement(17712..18389)
                     /locus_tag="VYQ24_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967829.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
BASE COUNT         5735 a         3175 c         4016 g         5497 t
ORIGIN      
        1 aataaaagat tttaggaaaa gtcgttatat catgctatac ctattctttg tggtataatt
       61 gcaagaggtt taatccgata atttataaag agagaaagat attcatgaga gatttattat
      121 ctaaaaaaag tcataggcaa ttagaattat tagaattatt atttgaacat aaacgttggt
      181 ttcatcgttc tgaactagca gagttattaa attgtacaga acgtgcagtc aaagatgatc
      241 tatcccatgt taaatctgct tttcctgact tgatttttca ttcttctact aatggtatac
      301 gcattattaa taccgatgat agtgatattg aaatggttta ccatcatttc tttaaacatt
      361 caactcattt ttcgatttta gaattcatct tctttaatga aggttgtcaa gctgagagta
      421 tttgtaaaga attttatatc agttcatctt cgctctatcg tattactagc caaatcaata
      481 aagtgattaa aaggcaattt caatttgaag tcagtctgac ccctgttcaa atcattggaa
      541 atgagagaga cattcgttac ttttttgcac aatatttttc agaaaaatat tatttcctag
      601 aatggccatt tgaaaatttt tcatcagagc cactatctca attgttagaa ttggtttata
      661 aggaaacaag ctttccaatg aatttgtcaa cgcatagaat gctaaagttg ctcctagtta
      721 cgaacctata tagaataaag tttggtcatt ttatggaagt agataaagat tcttttaacg
      781 accaaagttt ggatttttta atgcaggcag aaggaataga aggtgttgct cagagttttg
      841 aatcagaata caatatctct ttagatgaag aagttgtttg ccaattattt gtgtcttact
      901 ttcaaaaaat gtttttcata gatgaaagtc tctttatgaa atgcgtaaaa aaggatagtt
      961 atgtcgaaaa atcttaccat ctattgagtg attttattga tcagatttca gtcaagtatc
     1021 agattgaaat ggagaataag gataatctga tttggcatct gcataatacc gcacatctgt
     1081 atcgtcaaga gttgtttact gagtttattt tatttgatca aaaagggaat acaatcagga
     1141 actttcaaaa tatttttcca aaatttgttt cagatataaa aaaagagctt tctcactatt
     1201 tagagactct tgaggtttgt tctagttcaa tgatggtaaa tcatctatct tataccttca
     1261 tcactcatac caaacatttg gtgattaatt tgctacaaaa tcagcctaaa ctaaaggttt
     1321 tagttatgag taattttgat cagtatcatg caaaattcgt tgcagagaca ctttcttatt
     1381 actgtagcaa taattttgaa ctggaagttt ggactgaatt agaattatca aaggaatctt
     1441 tagaagattc atcttatgat atcattattt ctaattttat tattcctcct attgaaaata
     1501 agagactcat ctattcaaat aatataaaca cggtctcact catatatttg ttaaatgcca
     1561 tgatgtttat tcgattagat gagtaaccaa aaataacaaa aaaacgctat ttcgcgtttg
     1621 aactgtacct caaaagttag acagaaaaaa tctaattttt ggggtgtttt tgttaagaaa
     1681 agaccttcaa tcatttaagc aaaatgtaca tctagattta tagccctcta aaactcttgc
     1741 ttttttgtag aagatacgaa aaaagccata aaaccaaggc ttttcctgtt ttatgtagat
     1801 gtttacacat gaaaattgaa gtattttcaa aagctaattg gagtgatttt attcatgatg
     1861 ggataaatga tctggccaaa aatgattgag aaatagttgc ttaaaaagca tttgcatgat
     1921 actatatatt gaaaggaggt gataatatgg tagaacaaag aaaatcaatt accatgaaag
     1981 atgttgcttt agaagcagga gttagtgttg gaactgtttc acgtgtaatt aataaagaaa
     2041 aaggcattaa agaagtaact ttgaaaaaag tggaacaagc gattaaaact ttgaattaca
     2101 ttccagatta ctacgctaga ggaatgaaaa aaaatcgaac agaaacgatt gcaatcattg
     2161 taccaagtat ctggcatccc ttcttttcag aatttgctat gcatgtggaa aatgaagtct
     2221 ataagagaaa taacaaatta ctcttatgtt ctatcaatgg tacaaataga gagcaagact
     2281 atctggagat gttgcgtcat aataaagttg atggagtggt tgccattacc tatagtccaa
     2341 ttgaacatta cttgacgtca ggaattccct ttgttagtat tgaccgcaca tactcagata
     2401 ttgccattcc ttgtgtttca tcagataatg atgcaggtgg aagagaagca gctaaacaat
     2461 taattagtaa aggatgtcag catttagcct ttgtgggcgg acataataca accattaatg
     2521 aaaccaaacg tcgtagaatt tcatttgaaa agtatgtcca agagcataaa atacctctta
     2581 gtatttttga tttggatgag acagttactg actattatgg aaagctagag agtttcttgg
     2641 aagaaaattc ttctatagat ggaattttta caatcaacga ttttacagct ttggatgtga
     2701 ttgaaatact agaaaaaaat ggcaaacgta ttcctcatga tgtccaaatt attggatacg
     2761 atggaattaa aatggctgga gatagagatt atctactttc aactattgaa caaccattgg
     2821 aagaaatggc aaaagaagcg gttcgtattt tgtttgatat aattgatgga aagactgtta
     2881 atttgcagac aatcctacca gtaaaatttg ttgaaggaaa aacaacaaaa aatgaaaata
     2941 agtcttgaca gaaagcgcta tcaatgatag aatgaattca gataaaaaga tttattttta
     3001 aaacaaaaat gaaacgtttc aaaaaaagaa ataaagagac agcgccaagc gctatctttt
     3061 ctagaaaaaa atgaaacgtt tcaaaaagga ggttgctatg aatagcaaag cgaagcaagt
     3121 ttctctttgg gaaagaatca agaaacaaaa actcttgtta ttgatgactg tccctggttt
     3181 agttttaacc tttatcttta aatacatccc tatgtatggg gttttaattg catttaaaga
     3241 ttacaatcct ttaaaaggaa ttttagggag tgattggatt ggtttttctg agtttacaaa
     3301 attcatatcc tctcccaact ttggtatctt gttagccaac acattaaaat taagtatcta
     3361 tggtttattg cttggctttt taccaccaat cattcttgct attatgctca atcaactctt
     3421 gagtgaaaaa gtcaaaaaac gaattcagct cattttatac gcaccaaact ttatctcagt
     3481 tgttgttatt gtcggtatga ttttcctctt cttttcagtg ggaggaccaa tcaacaattt
     3541 tctttctatg tttggaatga aggctgactt cttgacaaat ccagacttct ttagaccttt
     3601 atacatcttt agtggtatct ggcaaggaat gggctgggct tcaacgctct acacggcaac
     3661 attggtaaat gtagatccag ccttagtaga agcagcccga ctggatggag ccaatatctt
     3721 ccaacgaatc tggcacattg atattccagc tcttaagcct attatggtta tccaatttgt
     3781 tttagctgca ggtggaatta tgaatgtcgg atatgaaaaa gcattcttga tgcagacatc
     3841 gttaaatttg ccaacttctg aaattatctc gacatatgtc tataaagttg gtcttgtatc
     3901 aggagactat tcttactcaa cagcggttgg tttgtttaat gcagtgatta acgtagtatt
     3961 gcttgttgca gttaaccaaa tcgttaaacg catgaataat ggtgaaggaa tttaaggagg
     4021 aaagtatgaa aaattcgatt atggatacaa aatttgatag acgtatctta ctcttaaata
     4081 aaatcattat tgtctttatc gttttgatga ctttgcttcc tttactttat atcgtcgtag
     4141 catcctttat ggatcctaag gttctggtta gtagagggat tagctttaat ccagccgatt
     4201 ggactgtaga aggttaccag cgtgtattca gtgaccaatc tattctaaga ggttttatca
     4261 attctctact atactctttt ggatttgcag ctttaacagt attgctatct gtgtttacag
     4321 cttatcctct ttctaagaaa gacttggttg gacgtcgttg gattaactac ttcttgattg
     4381 tgactatgtt ctttggtggt ggtttagtcc caacttactt gctcgtaaaa gaattgggaa
     4441 tgctcaatac tccatgggct atcattgttc caggtgctgt caacgtttgg aatattattc
     4501 ttgctagggc ctatttccaa ggattgcctg aagaattagt tgaagctgct gtcattgatg
     4561 gtgcaaatga tttacagatt ttcttcaaaa tcatgcttcc tcttgcaaaa ccaattatgt
     4621 ttgttctctt cctttatgct tttgtaggac agtggaactc atactttgat gcaatgattt
     4681 atatcaagga tccaaacttg gaaccattgc aacttgtact tcgtaaaatt ctcattcaga
     4741 gccaaccagg tcaagacatg attggagcac aagcggctat gaatgaaatg aaacgtttag
     4801 ctgaattgat taaatacgca actattgtca tttccagctt gccattgatt gttatgtatc
     4861 cattcttcca aaaatacttt gataaaggaa ttatggctgg ttcacttaaa ggataaaaaa
     4921 agaaaaaata aaaggagttt tctcatgaaa ttcaaaacat tctcaaaatc agcagttttg
     4981 ttgacagcta gtttagcagt acttgcagcc tgtggctcaa aaaatacagc ttcaagtcca
     5041 gattataagt tggaaggtgt aacattcccg cttcaagaaa agaaaacatt gaagtttatg
     5101 acagccagtt caccgttatc tcctaaagac ccaaatgaaa agttaatttt gcaacgtttg
     5161 gagaaggaaa ctggcgttca tattgactgg accaactacc aatccgactt tgcagaaaaa
     5221 cgtaacttgg atatttctag tggtgattta ccagatgcta tccacaacga cggagcttca
     5281 gatgtggact tgatgaactg ggctaaaaaa ggtgttatta ttccagttga agatttgatt
     5341 gataaataca tgccaaatct taagaaaatt ttggatgaga aaccagagta caaggccttg
     5401 atgacagcac ctgatgggca catttactca ttcccatgga ttgaagagct tggagatggt
     5461 aaagagtcta ttcacagcgt caacgatatg gcttggatta acaaagattg gcttaagaaa
     5521 cttggtcttg aaatgccaaa aactactgat gatttgatta aagtcctaga agctttcaaa
     5581 aacggggatc caaatggaaa tggagaggct gatgaaattc cattttcatt tattagtggt
     5641 aacggaaacg aagattttaa attcctattt gctgcatttg gtatagggga taacgatgat
     5701 catttagtag taggaaatga tggcaaagtt gacttcacag cagataacga taactataaa
     5761 gaaggtgtca aatttatccg tcaattgcaa gaaaaaggct tgattgataa agaagctttc
     5821 gaacatgatt ggaatagtta cattgctaaa ggtcatgatc agaaatttgg tgtttacttt
     5881 acatgggata agaataatgt tactggaagt aacgaaagtt atgatgtttt accagtactt
     5941 gctggaccaa gtggtcaaaa acacgtagct cgtacaaacg gtatgggatt tgcacgtgac
     6001 aagatggtta ttaccagtgt aaacaaaaac ctagaattga cagctaaatg gattgatgca
     6061 caatacgctc cactccaatc tgtgcaaaat aactggggaa cttacggaga tgacaaacaa
     6121 caaaacatct ttgaattgga tcaagcgtca aatagtctaa aacacttacc actaaacgga
     6181 actgcaccag cagaacttcg tcaaaagact gaagtaggag gaccactagc tatcctagat
     6241 tcatactatg gtaaagtaac aaccatgcct gatgatgcca aatggcgttt ggatcttatc
     6301 aaagaatatt atgttcctta catgagcaat gacaataact atccaagagt ctttatgaca
     6361 caggaagatt tggacaagat tgcccatatc gaggcagata tgaatgacta tatctaccgt
     6421 aaacgtgctg aatggattgt aaatggcaat attgatactg agtgggatga ttacaagaaa
     6481 gaacttgaaa aatacggact ttctgattac ctcgctatta aacaaaaata ctacgaccaa
     6541 taccaagcaa acaaaaacta gaggttgatt atgggagata agaaatacac agtagaaaaa
     6601 gccaatcgtt ttatagcaga aaataaacat ctcgttaata ctcaatataa gcctgaagaa
     6661 catttttcag ctgagattgg ttggatcaat gatccaaatg gatttgtcta ttttcgtgga
     6721 gaataccatc tcttttatca attctatcca tatgatagtg tttgggggcc tatgcactgg
     6781 ggacatgcta aaagtaagga cttggtgact tgggagcact tgccagtggc acttgctcct
     6841 gaccaagatt atgaccgaaa tggttgtttc tcaggctctg ccattgtcaa ggatgatcgc
     6901 ctctggctca tgtacactgg gcatatcgaa gaaaaaaccg gtgtccgcca agtgcaaaat
     6961 atggcatttt cagatgacgg gattcacttt gaaaagattt cccaaaatcc agttgtaact
     7021 ggaacagact tgccagatga gttgattgct gctgatttcc gtgacccaaa actctttgaa
     7081 aaagatggac gctattactc cgtagtagct gccaaacaca aggataatgt gggctgtatc
     7141 gttctactag ggtccgataa cctagtagaa tggcagttcg aatccatctt tttaaaaggg
     7201 ggagaacacc aaggctttat gtgggaatgc ccagattact tcgagttaga tgggaaagat
     7261 tgccttatta tgtcacccat gcgttatcag cgtgagggag actcatatca taacatcaac
     7321 tcatcgcttt tgttcacggg taaggtagat tggggagaaa aacgttttat cccagaatca
     7381 gttcaagaaa ttgatcatgg ccaagacttc tatgcgcctc aaacattgtt ggacaatcaa
     7441 aatcgtcgta tcctgattgc ttggatgcag acatgggggc gtacccttcc aacccatgac
     7501 caagaacaca aatgggcatg tgccatgact ttacctagaa ttctaagatt ggaagatggc
     7561 aaactaagac aattccctgt taaaaaaggc caatatcaaa tccaaataga taaagattgt
     7621 cattaccact taggaaatga tatagattat cttgaatttg gttatgacag taatgcgcag
     7681 caagtttaca ttgatcgtag ccatcttatt caaaaaattc taggtgaaga agaacaggac
     7741 actagtcgac ggtatgtaga tattgaagct aaagaattgg aagttgttct agataaaaat
     7801 tccatcgaga tttttgtcaa tcaaggtgaa gcaagcttga ctgcaactta ttacttaacg
     7861 gtgccagctg agctatcacg aattgattaa aaattaagtt atttctccta aagaaaaagt
     7921 tctctttcta aaatagtgga aagaggactt tttgtgtttt gggtatataa gcttagttta
     7981 tggtatttgt aaaattggtg ttggattatg atttaaacta gttttctaaa gaatttgaaa
     8041 aaaattttat ttaagcaaaa aaaagcccta tattataaga cttttcctgt tgtatttaaa
     8101 tgccccctac agggatcgaa cctgtgaccc acggattaag agtccgctgc tctgccagct
     8161 gagctaagga ggcaaagaaa aaagctgtat tggtgccgaa acttcacggt ttgtattgaa
     8221 cccgcgcaat taagcaggtg ggcaactcgc tctaactgaa gctgtttccg tgtgagacgg
     8281 cctacatgct gttagaagac ttttgtttcc ctaataatac aaaaaatagt cggtcaacac
     8341 ttaagtgtga agtcgtacac cacagcgttt ctatatttat atgataccac tttttcaaaa
     8401 aaaatcaaga ggaaagtgca attttttgaa aaggatttca gatttttcgc aaatggtcga
     8461 tagcttcctt tctttgagcc aaagcccgtt catatttacc agtatcttct gcttggaaat
     8521 agtgatgatt acgaattttt tctggcagat agtcttgctt tacccaattt ccaggatagt
     8581 tgtgtggata gagatagtct tgggcattcc ccagttcctt gcttccactg tagtgcccat
     8641 cacgtaggtg tcgcggaata ggcaagtgcc ctgatgtttt gaggtcagca agtgccttat
     8701 ccatagctac ataggctgag ttggattttg gagaaagggc caaatcaatc acgacattgg
     8761 caatgagaat gcgggcttct gggaacccaa tcttctgggc agcatccaga gcagtcacgg
     8821 tatgaatctg ggcctcagga ttggctaagc cgatatcttc ataggcgata acagtcaagc
     8881 gacgagcgag actaggcaga tccccagcct caatcaagcg ggcagtatag tggagactgg
     8941 catccacatc tgagccacgg atagactttt gcagggcaga tagaacatca tagtgaccgt
     9001 ctccatcctt gtccatagtg atgtagcttc tctgaagact attttccatg atgtctaagg
     9061 tgatgtggcg aatgccctcg tcattctcag gggtagagag aacagccaag tccagtgagt
     9121 taaaggcaga gcgaaggtct ccgtttgtag aggttgcgat gaaatccagc gcatcctcat
     9181 ctagttctat tggaaaatca aaaccacgtt cagggttact tagagctatc tgcagggcct
     9241 ctttgacgtc ttggttagac agaggttcca actcaaaaat ttgaacacgg ctacgaatgg
     9301 caggagtgac ggaaaagaaa ggattttcag ttgttgctcc aatcatgatg accagtccac
     9361 tttccaagag aggcaagagg aagtcttgct tggtcttatc tagtcgatga atttcgtcta
     9421 gcaataggac gagaccacca gaaaatttag cttcttccga gatttcttgc agtcgctttt
     9481 tactatcaac tgtcgcattg aaagttcgaa aggcatactt ggtcgttcca gcgatggcag
     9541 aggcaatact ggttttgccg attcccggag ggccatatag aatcatggag gacaggcggt
     9601 tggcttccac catgcggcgg atgatttttc caggtccgac cagatgctcc tgaccgatga
     9661 cctggtcgat ggttttaggg cgcatgcgaa gcgcgagatt gtctggcata gcagtccttt
     9721 ctagcgtgga tttgagtaga atgactcatc tactatatct tactatgtcc attctagcct
     9781 attagttgtg atatttcaat ggttttgtaa taatcaggga gtgagataga atctttttta
     9841 ctatttaatt taatgaggag aggtatcagc atgagagatt tgattttcct taaagctcaa
     9901 aataggatag ttgagcctaa agaagtggaa gtgctgactt gagaaataat attgctgaat
     9961 gtcattgtat gaactataat gaaaagagag gtgataagca atggaaatga tggaactacc
    10021 aagtcaggaa attttgattt ttacaaaaca aattcgtcat tggattctta gtgatcaagt
    10081 tatttcaggg aaaagaaaac ttttcttccg ggaagatact ccaaaagaga ttttagattt
    10141 gtacgaaaac attaaatcta aacttgattt tgcttaccaa gaagttcatt ctaataatgg
    10201 gttgaaaaaa tatgaaaaat agaataattg atgtttttga agtagtaaat cgcctccttg
    10261 taataactgt tgaaaatcct gatttcgaag acttgagagt taatcagttt gtaaaaattg
    10321 gagataaaaa gtatagagta cgcagtgttc ctatgattca ttcaactccg cctcaatctg
    10381 tcttagatcg agatactttt acaattgact atacagatga tgaattgctc gataaagaag
    10441 cagtttttac tactcattag aaaaataggt atgaataaat tggcttccct tagaaccaca
    10501 tctaggggct tttatgtata atagaataat aagtatcact cctttacaat acgtgtaaca
    10561 cgtgttataa tggtgttgtc aggaggtaaa agcgctatgc ctatgacaca aaaagagatg
    10621 gttaaactcc ttacggccca tggttggata aagactagag gcggtaaagg ctcccatatt
    10681 aaaatggaaa agcaagggga aagacctatc acaatccttc atggtgagct gaataagtac
    10741 actgaaagag ggataagaaa gcaagctggg ttgtaaaatt cagcccctgc tttttgatat
    10801 ggaggtagtg aaatgttagt tacgtatcca gctttatttt attatgacga cacagacgga
    10861 acagaagcga cttattttgt ccatttccca gattttgaat actcagctac acaaggagag
    10921 gggatttctg aggctttggc tatggggtcg gagtggctag ggataactgt tgcagatttg
    10981 atagagagtg atggagagtt acctcagcca tcagatatca atagcttgtc tttaattgat
    11041 aatgatcctt ttaaagatga tgaagatttc gtgtcaacct atgaccttga taaatctttt
    11101 atttctatgg tatctgttga tgtatcagaa tacctaggaa gtcaggaacc cattaaaaag
    11161 actttgacca taccaaaatg ggcagataag ttgggacgag aaatgggact taacttttct
    11221 cagactttga cagacgctat tgcagataag aaagttcaag cctaagatta agcgtggtat
    11281 cgtggtaata gtcaataaaa aagcacgtcc acttgtgcta gttactcgcc tattgatttt
    11341 atagatttat tagccctttc gagggctttt tatattgatt tttatgagaa atgaagaaat
    11401 cagactttta agaaaatcac tgataccaag ggtttaaatg aggtaatatg gtataattag
    11461 gacataaaat aattttgtgg taagatggta gtatctattt tagcatattt ccgagcaatc
    11521 ggggcgatta aagagtcgca tagaaagagg acaaaatggc aacatacgga tttttagata
    11581 ttttagagga agagttggac aagaactttc cctttgactt tgagattagt tgggacaagc
    11641 gtaaccacgc ggtagaagtg agttttctat tggaagcgca aaatgctgca ggtgtggaga
    11701 tggtggatga agatggagag gtttcatcag atgacattct ttttgaggaa gcggtccttt
    11761 tctacaatcc tgccaagtca accgtcaatg cagaagacta tttaacggtt atcccttatc
    11821 tacctaaaaa aggattttct cgcgagtttt tagcttattt tgcccttttc ctcaaagata
    11881 ctgcggaggt tgggctagat accctcatgg actttttgga agatccagaa gcagaggaat
    11941 ttgttatgga atggaatcaa gaagtctttg aagaaggaaa agttggcttg gaagagggag
    12001 aattttatcc ttatccaaga tactaggagt tggttggagg tgttatgaag aaaattggga
    12061 cttatttggt ttatgtgcta gctgttgtct ttattatgct agcttttgct tgtggaacaa
    12121 tcgcatttgc agagttgggg tattccgcag ttttagcctt tacttttggt tatgccttcg
    12181 ctcttctaag catgtattta atctttattc ttcatgagct gggtcatgct ttttgtggct
    12241 acttgacagg ctatcggctg gtggcttttg gattaggaca ttttattttg accaaaaagt
    12301 caggcaggtt tcatcttagt agaacagcca ttctgaaaaa tgttggtgct caatacattg
    12361 gtttaaaaga ggatgaaagc gatcaaagaa tcatcctgat gctttcagga ggcttgatgg
    12421 ttcatctcgg cttgatatta ttggcgatag tgtttggatt tttgacaaga agctggtatt
    12481 ttgcagggac ttggattttt cttaatttat ctttcttcct aaataacatt ttgccagtcg
    12541 acatcaccga tggagcaaaa atttgggaat tactacaaca ccctgaaaat accaaatacg
    12601 cctacctgat gttgaggcat tctgcccaga ccttgctagc tcctcaagaa tatgatttaa
    12661 aagactttgt tcaagcagtt cctgaggatg cacgagggag ttttgcggat ggtgtattag
    12721 gtatgcaagg tgaagtttcc attttggaag ggaaggaaga agtagctaaa cagcagtttc
    12781 aagcattact tgagaggaca actactccga tgatgcagac agttgcccaa ctatccctgc
    12841 ttcatatcgc cttgttggaa ggagattttg agcaagcgga gcaatatgct agtattcgac
    12901 aggtcaaatc gtttctgtct ctcaaactgt ccaatcttca agtgatacaa gcttggtatc
    12961 aatttaaggt aaagaaagat gtagatcgaa ctcgtaaggc tctcaagatt gctagacaga
    13021 aaatggatag tagtcgaatg ttgagggacg agaaacgcta ttatgaaaac ttgttagcag
    13081 agctggaaaa ggaaatgtta gaaggaactg aagatggaac tagaaatttc tgatttccaa
    13141 ggttgcaaga ttgccctctt ttgtggggat aagctactga cgattttgcg tgatgacaag
    13201 gacgatatcc cttgtcccaa catgtgggag ttgccaggtg gtggccgtga aggggacgaa
    13261 agcccttttg aatgcgctcg gcgtgaagtt tatgaggaac tgggaattca tttagatgaa
    13321 gactgtctgc tttggagcaa gatttatcct agcgtaattt ttaaaggcaa gaaatcggtc
    13381 tttatggttg gtcagctaag acaagaacaa tttgacaata tcatcttcgg agatgaagga
    13441 cagggctata aattgatgcc tattgaggaa tttctcaatt ctaaacaggc agtgcctcag
    13501 ttgcagggaa gattgagaga ttatttggag gaaagtttat gataacactt gttcgagcgg
    13561 gagcagagga tttagaaact attattgcaa ttcagagagc cagctttaag gctgtttatg
    13621 aaaaatacca tgatgaatat gacccttatc tcgaggactg cgaacgaatt aagtggaaat
    13681 tagtcgagcg tcccaatagc tattactact ttgtaaaaga agatgaagaa aacatcggat
    13741 ttttacgagt tcagaccaat gccgagttaa cgaaagcttg gttgggaacg gcagcgattt
    13801 tgcctcagta tcaaggaaaa gggtatggat ctaaaggttt aagcttactg gaaaaagaat
    13861 ttccaaccat cacacaatgg gatttgtgta cggtgttaca ggatgcaggc atggttgctt
    13921 tttacgagaa aaacggctat cgtcaaatcc acattgaggc tgaaaaagaa ggcatggata
    13981 tggtctacat gaaaaaattg atagagaaat agaaaggatg gttcggttaa atttctaaac
    14041 tgaacccgcc ctaaacacgg tgccaaaaag ataaacttct cttagacaca aacgtcttca
    14101 gagagtttcc tattttggct ttgtgtttta cgggcttggt atcttaataa tggaaacatg
    14161 gcaagagtta aaagttacag tgaagcgtga gggagaggag ttggtttcca atctcttgat
    14221 tgagttaggt gcccaagggg tagcaattga agacagtatg gactatgtgg gaaatgtgga
    14281 ccgattcggt gaaattttcc cagaggtgga gcaacaggaa gaaatcgtag tgaccgccta
    14341 ctaccctgaa acggttgatg tagcagcagt tgaagcggat ttgcaggctc gtctggcaga
    14401 attgaccgac tttatggacc taggagaagt caagatgggc acgactgcct tggctgagga
    14461 agactgggct gacaactgga agaagtatta tgaaccggca cgcatcaccc atgacctgac
    14521 catcgtaccg tcatggacgg actatgaggc aacagcagga gaaaagatta tcaagctgga
    14581 tcctggtatg gcctttggga cagggaccca cccaacaacc aagatgagcc tctttgcctt
    14641 ggaacaggtt cttcgtggtg gcgaaacagt gctagatgta gggactggtt caggggttct
    14701 ttctattgca agctcactcc taggtgccaa ggagattttc gcctatgact tggatgatgt
    14761 ggcggttcgt gtcgctcagg aaaatattga gctcaacact ggtatggaaa atatccatgt
    14821 agcagcagga gacttgctta agggagttga gattgaagca gatgtcatcg tggctaatat
    14881 cttggcggat atcctcattc atctaataga cgatgcctat cgtttggtca aggacgaagg
    14941 ctacctgatc atgagtggca ttatcaagga taagtgggac atggtgcgcg agtcggctga
    15001 atcagctgga tttttcctcg aaactcacat ggttcaaggg gaatggaata cctgtgtctt
    15061 taagaaaacc aaggatatct ctggtgtgat tggaggctag catgcagcag tattttgtaa
    15121 aaggcagtgc tatctctcct gtcactatcg aggacaagga aaccagtaag catatgtttc
    15181 aggttatgcg cttgaaagaa gaggatgagg ttaccttagt ctttgatgat ggcatcaagc
    15241 gcttggcgcg cgtgctggat atggaaaatc gtcagtttga gttggtccaa gaattaaatg
    15301 acaatgtgga actaccagtc caagtgacca tcgcatctgg atttcccaag ggagacaagc
    15361 tggagttcat cgctcaaaaa gtaaccgaac tgggggctag ccaaatctgg gcctttcctg
    15421 cagactggtc agttgccaag tgggatggca agaaattggg taaaaaagtt gaaaaactag
    15481 aaaaaattgc ccttggagca gccgagcaaa gcaagcgtaa tattgtacca agtattaaac
    15541 ttttcgagaa aaaagcagat tttctagctc agctggacca gtttgactct atcatagtag
    15601 cctatgaaga atcagctaaa gaaggagaag ccgctgcgct cttacaagcg gtctctggtc
    15661 ttgaaacagg agccaaactg ctctttatct ttggaccaga aggcggtctc tcacctgcag
    15721 aaatcgaaag ttttgaagct aagggagccg ttttggtagg cctaggtcct cgtattttgc
    15781 gagcagaaac agcaccgctt tacgccttat cagcccttag tgttttagta gaattagaga
    15841 aataagagga agaaaatgga acaaaaacac cgttcagaat ttccagagaa ggaactctgg
    15901 gacttaacag ccctatacca agaccgtgag gatttcttgc gtgcaatcga gaaagctcgc
    15961 gaagacatca accagtttag ccgtgattac aagggcaatc ttcacacttt tgaggatttc
    16021 gagaaggcct ttgcggaatt ggaacagatc tacattcaga tgagccatat tggcaactat
    16081 ggttttatgc ctcagacgac ggactatagc aatgacgaat ttgccaatat tgcccaagct
    16141 gggatggaat ttgaaacaga tgccagcgta gccttgacct tctttgacga tgccttggtg
    16201 gtagcagacg aggaagtctt ggaccgtttg ggtgaattgc ctcacttgac ggcagctatt
    16261 cgtcaggcca aaatcaaaaa agcccactat ctaggggctg atgtggagaa ggccttgacc
    16321 aatctcggtg aagttttcta tagtccgcag gacatttata ctaagatgcg agctggggat
    16381 tttgaaatgg ctgactttga agcccatggc aagacctaca aaaacagctt tgtcacctat
    16441 gagaatttct accaaaatca cgaggatgcc gaggttcgtg agaaatcctt ccgttccttc
    16501 tcagagggac ttcgtaagca ccaaaatacg gctgcagcag cctatctggc tcaggtcaag
    16561 tctgaaaaac tcttggctga tatgaaggga tacgactctg tctttgatta tcttctagct
    16621 gaacaagaag tggaccgtgt catgtttgat cgccagattg acctcatcat gaaggacttt
    16681 gcaccagtcg ctcagagata cctcaagcat gttgccaagg taaatggtct tgaaaagatg
    16741 acctttgcag actggaaatt ggacttggac agcgccctga atcctgaagt gactattgac
    16801 gatgcctatg atttggtcat gaagtcggta gaacctttgg ggcaagaata ttgtcaggaa
    16861 gttgctcgtt accaagaaga gcgctgggtg gactttgctg ctaacagtgg caaggattcc
    16921 ggtggttatg cggcggaccc atatcgcgta cacccttatg tactcatgag ctggacaggc
    16981 cgtttgagcg atgtctatac cttgattcat gaaatcgggc attctggtca attcatcttt
    17041 tcagacaatc atcaaagtta cttcaatgcc catatgtcga cctactatgt tgaagcaccg
    17101 tcaaccttca atgaattgct actcagtgat tacttggaga accagtctaa tgacccacgt
    17161 caaaaacgct tcgctctggc tcatcgcttg acagacacct acttccataa ctttatcacc
    17221 cacctcttgg aagcagcctt ccagcgtaag gtgtatacat tgattgaaga aggggagacc
    17281 tttggagcaa gcaaactcaa cagcattatg aaggaagttt tgacagattt ctggggagat
    17341 gctattgaaa ttgatgatga tgcagctttg acttggatgc gccaagctca ctactatatg
    17401 ggcttgtata gttacaccta ctcagcagga ctagtcatct cgacagctgg ttaccttcat
    17461 cttaaaaact cagaaactgg agctgaagac tggcttaatc tcctcaaatc aggtggtagc
    17521 aagacaccac ttgagtcagc catgattatc ggtgctgata tttcaacaga caaaccactc
    17581 cgtgatacca tccaattctt gtctgacaca gttgaccaga ttatcgccta cagtgctgag
    17641 ttgggggagt aaggggatag agaggttgtt atagaatggg tatggacagt tatttgctta
    17701 aaagtccatg tttaaatccc ccattccttt tcgaggaatt ctttgccctt ctctgtaatg
    17761 gtaagtgaac gtggcgtttg tgcagggatg accaattgtt tttcactgaa aaattctaaa
    17821 agagcattgc cgagagctcc gccgacatga tttttacgtt cgctccaatc caaacattta
    17881 gggcaaaatt tccgtttttt ctttttcaat tcatccgtat caatgtgtaa ttttctttgg
    17941 aaataagact tcccagcttc agtgagttgg aagtcttttt ctgattcgct gaggagattt
    18001 tcttgcaaga gataattggt aatggaaatg ccaagctcac cagcaatatg gtcatagcag
    18061 gtgcgagcga cctttaattc ctttttgtga atgtgttgtt tataactttt gacatcacgc
    18121 tgaggagagt agaagctgag aaattccagc atttctgcaa tggcagcact ttttaaagaa
    18181 taataatgaa aacgtccata ggtttgtaga gtggttacct gcgcttcggt aagtaatttg
    18241 aggtgatagc tgacagtatg ttgcttgatt ttggtaaagc tggcaatttc gttaaccgtg
    18301 tgagcttttc catccattag aatatctaaa atgtgcatcc gagaagggtc tgataagagt
    18361 tttgtgatga tgaaaatatc tggttccata aaaattttct ccaaatttca tatatcgatt
    18421 att
//
LOCUS       DAVMEI010000024        17885 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVMEI010000024 DAVMEI010000000
VERSION     DAVMEI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.024"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(163..309)
                     /locus_tag="VYQ24_000658"
     CDS             complement(163..309)
                     /locus_tag="VYQ24_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967830.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            complement(306..761)
                     /locus_tag="VYQ24_000659"
     CDS             complement(306..761)
                     /locus_tag="VYQ24_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967831.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEVTVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            complement(898..1782)
                     /locus_tag="VYQ24_000660"
     CDS             complement(898..1782)
                     /locus_tag="VYQ24_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1967832.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(1786..2172)
                     /locus_tag="VYQ24_000661"
     CDS             complement(1786..2172)
                     /locus_tag="VYQ24_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1967833.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(2165..3508)
                     /locus_tag="VYQ24_000662"
     CDS             complement(2165..3508)
                     /locus_tag="VYQ24_000662"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1967834.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRFFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3590..4474)
                     /locus_tag="VYQ24_000663"
     CDS             complement(3590..4474)
                     /locus_tag="VYQ24_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967835.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4486..5103)
                     /gene="cysE"
                     /locus_tag="VYQ24_000664"
     CDS             complement(4486..5103)
                     /gene="cysE"
                     /locus_tag="VYQ24_000664"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1967836.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(5119..7332)
                     /gene="pnp"
                     /locus_tag="VYQ24_000665"
     CDS             complement(5119..7332)
                     /gene="pnp"
                     /locus_tag="VYQ24_000665"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1967837.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDIIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            complement(8017..8883)
                     /gene="metF"
                     /locus_tag="VYQ24_000666"
     CDS             complement(8017..8883)
                     /gene="metF"
                     /locus_tag="VYQ24_000666"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1967838.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQYMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     NAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8947..11196)
                     /gene="metE"
                     /locus_tag="VYQ24_000667"
     CDS             complement(8947..11196)
                     /gene="metE"
                     /locus_tag="VYQ24_000667"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1967839.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSFVAAYQEVFAKLADLGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLFQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGKNAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(11457..11723)
                     /locus_tag="VYQ24_000668"
     CDS             complement(11457..11723)
                     /locus_tag="VYQ24_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1967840.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(11849..12296)
                     /locus_tag="VYQ24_000669"
                     /pseudo
     CDS             complement(11849..12296)
                     /locus_tag="VYQ24_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            12452..13453
                     /locus_tag="VYQ24_000670"
     CDS             12452..13453
                     /locus_tag="VYQ24_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1967841.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            complement(13521..15923)
                     /locus_tag="VYQ24_000671"
     CDS             complement(13521..15923)
                     /locus_tag="VYQ24_000671"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1967842.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISFGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(16000..16509)
                     /locus_tag="VYQ24_000672"
     CDS             complement(16000..16509)
                     /locus_tag="VYQ24_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967843.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(16509..17555)
                     /gene="pheS"
                     /locus_tag="VYQ24_000673"
     CDS             complement(16509..17555)
                     /gene="pheS"
                     /locus_tag="VYQ24_000673"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1967844.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT         5005 a         4044 c         3468 g         5368 t
ORIGIN      
        1 gtgactatac ttttcatatt tataattcat tcctttcgtt tcactcaagg cacaacacag
       61 aatgaaaaag tgttgtgatc tttattttgt tttataataa tagtgagaaa gcctatcact
      121 actacaaatc acggggaggt gaataagtga gtggtacagc cactacctcg catattttgt
      181 cacatcattt aacggtacat aataagttgt accatctgaa taagttgcta caatatcatt
      241 tgcatgctct ccttcacctt tagcaaaggt tggagctcct gctggatgat ttttatttgc
      301 ctctttcaat ttttcaataa tggcattttt tctgtatctt ttatattatc aggatttttc
      361 actaagattt tgtctggata tgtcggttta gcagaaacaa tttttactgt tacttctttt
      421 ttattcgaag cacttgtcca gtttccagca ttatctttag catttaattt tacagtaatt
      481 cctgaactag gaacttcagt agcaggttga ttatcaacat tattcaactt taatttcaaa
      541 agagctgttg catcagacgt tttatcaatc gttatatata atgatgaatt gttattataa
      601 acagttcctt catatttagc tgtttgtgag ctacttgaaa cagaactgaa attataccca
      661 ctacctccct gattatcttc aatgcttacg tctaaatgaa cttccccact attatttggc
      721 ttagcaacaa ctgttatagt aaaataacat aaaatttgca taaatagatt agggaaatca
      781 aagcagcttc taggaatgtt ttagcagtca cagtgtactt tcccagcatc aagccactat
      841 aactctgcac ataaaaatgg agaagatggc aatcctcttc tccaaatatt aacttcttta
      901 caaaccaact atagttgaca aagaacctaa aatcaattga taacacaagg tcaggtcggt
      961 caactctttc aactgaagcc ctgtcaactc ttcccattta tcaatcttgt attggagaga
     1021 attgcggtgc agatagagtt gctgggctgt tttagtgaga acagcactat tttcccaaag
     1081 agagagaatg atttcctgaa tctgatcttg atccaaaatc atctggtgta gacattcctt
     1141 gattggcttc aagtccacga gtctttctcc catactccaa agatagagct gagaaaaagt
     1201 atgaacacct tggtgaccct gacgccacca tgtcttgaac aaatcccgct cagctttgat
     1261 taagtctgat agggcttgat gtcccgtctg agaccaaacc tgacccaaca tgatagaaag
     1321 acgaagtcca aagtcatact caaccgcttc aatcgtatca cttaaaatat ctcttacaga
     1381 agtgtatttg tcttgttgaa gcacgaaaac ataatcctga gctccgacct gtagcactgt
     1441 ctgacaattc ggaaaaagag tccgcatcat atctagccaa gaagccagat tttcctgctg
     1501 aaaataagaa agatggcaat aaaccaactg aatcttttta aaaacttgcg gtgcctgtcc
     1561 cttgccctca accagatagg aataccaagg gtttagcgaa cgagcctgct cctgctgggt
     1621 caaaagggca accaactgct tttcacgctc gctgagccca gcttcctcca gcaaaatcca
     1681 ctgctgagaa gctaaaggga gcgtgagata gccctctttc tctactggtt ggtctgaaat
     1741 ccgagcctca ggaaaccagt cttgtagttc ttttgccctc atgttctagc cctccacttt
     1801 ttggatgcac catgaaacca aactctcaag acgttccaga ttctcagtca tatggagata
     1861 gcccataacc gcttcaaatc ccgtggacat acgataagtc acgacatctg catttttagc
     1921 ctttgtgtgg ctattggtat tgcggccacg tttgtagatt tcttcttctt tttccgttag
     1981 gacctgctcc tccaacatga gagcaatcag gcgagcctga gccttggctg acacgtactt
     2041 agttgcttct tgatggagtt tattgggttt ggtcatacct ttgaggatga ggtgacggcg
     2101 aatatacata gaatacaccg catccccctc aaaggctagc gcaatcccgt taatgagatt
     2161 gacatcaatc acgtgtccac ctcactccat ccttggtatc aaggagctta attccttgag
     2221 taaccaattg gtcacggatt tggtctgctg tcgcaaagtc acgattggca cgcgcctctt
     2281 ggcgtttttg aatcaagtct tcaatctctg catccaaaac ttcctcaaca aagacaattc
     2341 caaaaatttc taacatatct gcaagagctt gcttgacact tgcatcatag ttccctgagt
     2401 tgatccattt ggccatttca aagacaactg tgataccgtt ggcagcatta aaatcttcat
     2461 ccatagctgc tacaaactta tctttaaagt tttgtaactc ttgggcatcc acatttcctg
     2521 taaatggttg ttcgtaagta ttcttgagat acttgagatt ggtctcggca tcgcgaactg
     2581 ccttttccgt gaagttgata ggcttacggt agtgctgggt cgcaaagaag aaacgaagta
     2641 cttgcccatc aagagtttta agggcatcgt gtaccgtaat gaagttaccc aaggacttag
     2701 acattttgac attgtcgata ttgacaaagc cattgtgcat ccagtagtta gcaaaagcct
     2761 tgcctgtttt agcttcagac tgggcaattt cattggtgtg gtgtggaaac tctaggtcag
     2821 ctccaccacc gtggatatca atggtatcac ctaaaatctc tgtcgacatg actgaacact
     2881 caatatgcca acccggacgt ccaggtcccc aaggactatc ccaagaaatc tcacctggtt
     2941 tggaagattt ccatagagca aagtctacag gattttcctt acgagccgtt tcttcatcgg
     3001 tacgacctga agcacctagc tccaaatctt ccaaggtttt attagccaat ttagcatagt
     3061 tgtgggattt ttctacacgg aaatagacat ccccttgact ctcataggca aagcctttct
     3121 cgatcaagtc ttccacaaaa cggatgatgt ctgccataaa ctccactaca cgcggatggc
     3181 gagtcgcagg tttcacgccc aatgccgtca catcctcacg aaaggcagcg atgtacttat
     3241 ccgcaacctc ctgaggcgtg ataccttctt ccctggcacg gttgataatc ttatcatcca
     3301 catctgtaaa attggaaata taggcaacct tatacccacg gtactcaaaa aagcgacgaa
     3361 tcgtatcaaa agctaccgtc gaacgggcgt ttcctacgtg gatatagttg tacaccgttg
     3421 gcccacaaac atacatcttg atcttgccgt cctcaatcgg gacaaattct cgcaaatcac
     3481 gagacatggt gtcatagatt ttaatcataa atcataatca ggaaagctga aatccaagaa
     3541 caattagttt catcactaaa agttcaagta aatttcagtc cgaatatctc tacacttcgg
     3601 aatcccttgc tcctttctca ttcagataaa ccacctgagt ctgtttgaca aagccaattt
     3661 tttcatacaa acgtttggca cctacattgc tatcttccac tgcaatctga aattccttgt
     3721 cattttgctc aattagttgg ttgacgaggg attttgctaa gtagcttcca tagccttttc
     3781 cacgttcagg ttccaatatt gctaaaccgt agaggtaatt cgtattagtc gataaatcaa
     3841 ccgtacaagt tccaataacc tgaccagctt ttaataaaat atatagtcgg ctttctggat
     3901 ctttcagagc ttcagcgaca tatctatcca caacttctct cgattcatgt tcctctgaaa
     3961 atgcctgaaa ttttaattga ctaatttgat cctgatacga actatctgct aacaaaactt
     4021 caagatggga aacatttgct aacggataag gtcttctatc cttacctaac caagtttctg
     4081 tctcttcatc ctcgattagt ccccagttac tggcaaagtc aggatgattc tctaaaaaaa
     4141 tacgttctgt ctgaaaagtg actgaccgaa tggggaaaga agctgtttct ctctcaaaac
     4201 tagtaaacaa tgcacgcgca atcccctgac ggcgatgacc tggatgaacc agtatcgtca
     4261 cttctacatc ttggtcatct gcatagacag ttaataaacc aacaagttcg cctttttcat
     4321 aataaaggaa aaaggcgggc atgtttgggt caaaattaag catgttagag agatagggat
     4381 cgcgataggt accgtcatag ttttggcaac agttaattac ttttttcgcc tcagatagct
     4441 cctcttggct taacttgttt cttgcttgaa tcatataggt atcctctaca aaccagacga
     4501 tctgtgactg gcatctttag cctgctcgag tttattgaca taatactctc gtttttcttc
     4561 gacttcgtga atgacaggct catctttctt accatgaaga cggacaatct tggccggaat
     4621 accgacaacc gtcacgtcac taggtacatc tgctacgaca actgctgcag caccgacctt
     4681 ggcattttca ccaatttcca caggcccgat aacttgggca tgggctgata tgagggctcc
     4741 ctttcgtaca gtcggatggc gtttgccaca gtctttccct gttcccccga gagtcactcc
     4801 gtgatagaga agaacgcctt tttcaacaat cgctgtctct ccaatcacca gaccagaacc
     4861 atggtcaata aaaacacctg aatcaatctg ggctcctgga tgaatctcaa tctgagtcca
     4921 aaagcgccaa aactgactgt acatacgagc taatagtttg aagccgtgct tccagagaaa
     4981 atgcgagaga cggtgggccg ccaaggcctt gacacctgga taagtcagca aaacctccaa
     5041 agtggtgcgg gccgctggat cattttcttt tacaatatca atggtttcgc gccaccaccc
     5101 catacatttc tccttttctt attctgaatc ttttgatgtt tctgtaaatt ctttcttagg
     5161 tttgtaatcc ttttgatgac gtgggcggtg agggcgctca gacttttcac ctttttcatc
     5221 atgctcaggt tttggcggac gaggtagaag agccttcata gaggcatcga tacggccttt
     5281 ttcatcaatt ttgataacct taacatcaac ttcatccccg atttctacca aatcctctac
     5341 acgattggta cgagtccaag ccatctcaga gatatgaaca agggcatctg tcttatcaaa
     5401 gaggttaaca aaggcaccaa atttctcgat acgaacgact ttagcacggt aaacttcatc
     5461 cactttggct tcacgaacca aaccagcaat aatttctttg gcacggttaa tagcatcttg
     5521 gtcactagag tagatagaca catttccttc ttcgtctata tcaatcttaa cacctgtttc
     5581 agcgataatc ttgtcgatgg tttctccacc cttaccgatg acaatcttaa tcttgtccac
     5641 atcaatcttg atcgtatcaa ttttcggagc agttggagcc aattctggac gaacttctgg
     5701 aatggttgct tcaatgatat caaggatttc aaaacgcgct ttcttggctt gagcaagagc
     5761 ctccgtcaag atttctgcag taatcccttg aatcttgata tccatttgaa gggctgtaat
     5821 cccatcacga gtacctgcaa ccttgaagtc catatctcca aagtgatctt ccaaaccttg
     5881 gatatctgtc aatactgtgt agttatttcc atctgagata agtcccatag caataccagc
     5941 tactggcgcc ttgattggca caccaccagc cataagggca agagttcccg cacagataga
     6001 agcttgagat gaagaaccgt ttgattccaa aacttctgct actagacgga tagcgtatgg
     6061 gaattcttcc aagcttggca agacttgagc aagagcacgc tcaccaaggg caccgtgacc
     6121 gatttcacga cgacctggcg caccgtaacg acctgtttcc cctacagaat attgagggaa
     6181 gttatagtgg tgcataaagc gtttcttgta ctctggatcc aaaccatcaa tgatttgagt
     6241 ttctcccatc ggagccaagg tcaagactga aagagcttga gtttgcccac gagtaaagag
     6301 acctgaacca tgtacacgag gaaggaagtc aacaaccgca tccaaaggac ggatttcatc
     6361 gaccttacga ccatcaggac gcaccttgtc ttctgtaatt aaacgtcgca cttctgcgtg
     6421 ttccatttgt tccaagattt cagccacatc acgcataata cggtcaaatt cttcgtggtt
     6481 cgcatatttt tcttcgtaaa cggcagtcac ttggtctttc actgcttgag ttgcagcttc
     6541 acgggccaat ttctcttcta cttgaactgc cttttggagg tcactgttgt aggctgcaat
     6601 gatttcagct tgcaattcag catccacgtg aagcaattcc acttctgctt tttctttacc
     6661 gacagcagca acgatttctt cttggaaggc aatcaattct ttgacagctt cgtgcccttt
     6721 aagaagcgct tccaacatga tttcttctga caattctttg gcaccagact ctaccatgtt
     6781 gatagcgtgc ttggttccag ctactgtcaa ttcaagaaga gattgctctg cttgttcttg
     6841 acttgggttg atgatgattt ggccatctac atatcccact tgtaccccag caattggtcc
     6901 gtcaaatgga atatctgaaa tagacagtgc caaagatgaa ccaaacatag cagccattgg
     6961 tgcagatgca ttttcatcat aagaaagcac tgtattgatg acttggactt cattacggaa
     7021 accttccgca aacataggac gaatcggacg gtcaatcaaa cgcgctgtca aggtcgcatc
     7081 tgttgaagga cgtccttcac gtttcataaa gccaccagga aacttcccag ccgcatacat
     7141 tttttcttcg tagttgactt ggagtgggaa gaaatcccca gttgccattt tcttagacat
     7201 aacggcagca gtcaagacag ttgactcacc gtaacgtacg acaacagatc catttgcttg
     7261 cttagcaacc tgaccagtct ctacaattaa ctcacgaccc gcaaaagtcg tttgaaacac
     7321 ttgttttgcc attttaatcc cctttggatt gatgaaatta tacgccttgc ctacaaagat
     7381 caagatacca aggacgtcaa aagcaaagta aaaataggaa actgacgaag tcttcgatga
     7441 agacaagaca gtttatcttt tttacacagc ttttcggccg tgttcaatta cacaagatat
     7501 tttggacggt tcggcttgcc gaacatttct gtagaaaaat aggaaggtga cgtcgcactc
     7561 gacgagtgct aggaagctta tcttttttcc taagaaatga gaccaaaatt caagtcatca
     7621 agataccaag ccgtcaagca actcaaagga agataggaaa tcgaacgacg gagcgactac
     7681 tcctagggag atttatcttt ttccacagag ttgtaggcaa gttcagtttt caagatacat
     7741 cattagaaag gtttaatact aaagtatcta aagctttcac gctaatcgct atcgggcgat
     7801 tagttaaatg ctttactaac tctctcgtca aataacatcg atttgactca ctcgtgtcgt
     7861 taaatcttac agtttaaatg cattgtatta tttaatacct tcatctttgt atcaagtacg
     7921 tacagaattt attttatcat atttttctta aaaagtgagg tctttaccat taaaaaggaa
     7981 ccattcccct cacctgagaa gaatggtttg cttttattat cctagagact ggtgattaaa
     8041 caaggcatgg gttgcttgat ggatgtattt tgctgtatca gcattattca tcgtatagag
     8101 atgcacaccg gcaacatcct gagttaccaa gtccacgatt tggtccactg cataggcaag
     8161 tcctgctgct ctgagcgact cagggtcatg ctcatacttg tctaagatgg ctttaaattt
     8221 gcgtggaaga tggatattct cacaagtctt caagagtcgg agagcctgat ttcgattcag
     8281 aattggcata attcctgcat gaatgggaac atcaatccca gccaagatgc acttgtcctg
     8341 aaaatcatag aagcgctcat tgtcaaagaa gagctgagtt acgaggctcg aacagcctgc
     8401 attcactttc ttcttaagat tttgaatatc tgaaatctga tttggcgaat ctggatgccc
     8461 ttctggatag caagctccaa taatatcaaa gtgaggggtt tgttccttga taaactcaat
     8521 caagtctgtt gcatagcgga aatccttttg tggttccacg tctggaataa tatccccacg
     8581 aagagccaag attttctgca ccccaacttt gtccaagtca gcaatagttt cagcaacctt
     8641 gtccttagtt agataaatag ctggcaagtg ggcaatggtc ggaatcgcca aatcattttg
     8701 gataaagtca gccaaacgaa ccgtcgtttc cttgatatta aatttattat tgctggcagt
     8761 tacactgata aaatggggag ccaactcctg catatactgc aaggctgaaa taatgttacc
     8821 attacccacg gctgggtttg gagggaacac ttcaaatgag agtgacggtg tttggcgtga
     8881 catatgtaat aaccttttct agttgatttc tttttgaaca accactgtat ggagagaaat
     8941 ccaatcttac aatttctcac gcgcagcttt agctgcttca acaaggcgga tcaagctttc
     9001 ttttgtttct gggataccac gtgttttcaa accacagtca gggttgatcc aaactttctt
     9061 gcttggcact ttagcaagga tggcttcgat tgtgttgtcg atttcgcctt catttggtac
     9121 acgaggtgag tggatatcgt aaaccccagg tcccacttct gtttggaagt ttttcgcttt
     9181 gagttcgtcc aagatttcaa ggtttgaacg gctagcttca aaggaaataa cgtctgcatc
     9241 catgttatcg atagctggga tgatatctgt aaattctgag taacacatgt gagtgtggat
     9301 ttgtgtgtct ggcgctactg ttgagtgtac caagcggaag gcaggaatag cccagtcaag
     9361 gtagtcttcg taccagtcgc tacggcggag tggcaatttt tcacgaagag cagcctcgtc
     9421 gatttggatg attttcacac cagcagcttc aaggtcaagt acttcatcct tgatagcaag
     9481 ggcgatttgg agagttgaat ccttgataga gatgtcttca cgtgggaatg accagttaag
     9541 gatggtaaca ggtccagtca acataccttt aacaggtttg tttgtacgac tttgtgcata
     9601 gctagaccat ttaacagtga tagggttaag acgagtgaca tcaccccaga tgattggtgg
     9661 ttttacccca cgcataccgt atgattgtac ccatccattt ttagagaaga ggtatcctga
     9721 caagttttga ccgaagtact caaccatgtc attacgctca aattcaccgt gaacaaggac
     9781 atcaaagtca atatcttctt gccacttgat ccattcgtca atcgtttcag caaggaaagc
     9841 gtcgtactct ttttgagaca attcaccttt acggtaagcc aaacgtttgg cacgaacttc
     9901 ttttgtttga gggaatgaac caatcgttgt tgttggaaga gctggaagtt tgaaagcttc
     9961 ttcttggata gcttcacgtt ctgcaaaggc tggcaaacga gtgtagtctg cgtctgtcaa
    10021 gccagcgata cgcgcacgaa gttcagcatt tttaccaaca cgctcagtcg caaagagttc
    10081 tttgttggct gcaagagctt ctgaaccttg accattgcgg atagcatcca agtcacggat
    10141 ttcatccaat ttttcaactg caaaggcaaa gtggttcaag agtgctggtt caaattcttc
    10201 attagcagtt gtaaatggca catgaagaag tgagcaagag cttgtcaaaa cgatgttttc
    10261 agctgggatt tgctcaagaa cagccaagct cttttcgtag ttgttgcgcc agatgttttt
    10321 accattgaca atacctgcat agagagtctt gtcagctggg aagccacctt taacgagttc
    10381 aagagttttc ttaccttcaa caaagtcaag accgatagca tctactggca agtttacaag
    10441 gtcagcgtat acgtcacgaa catcaccgaa ataagtttga aacaagactt caagaccttt
    10501 tttgtcagcc aagagtttgt tgtaaaggtt caagaagaga gctttttctt cagctgtcaa
    10561 gtcttttaca agagccgctt catccaattg gatgcgagtc gcaccaaggt cagccaattt
    10621 agcaaaaact tcttggtaag cagccacaaa gctatctacg aagtcgtctg ctttcacgcc
    10681 ttcttcaaag tctgacaatt gaaggaaagt gaagggacct acaagaacag gacgagtgtt
    10741 caatccaagt tctttggctt cttgaaactc atcaaaaatc ttgtgacctg caagtttgac
    10801 ttgagtgtct ttttcaaatt taggaacgat gtagtggtag ttagtgttga accatttctt
    10861 cattggaagg gcgcgaacgt cccctttttc tccctggtaa ccacgtccca aagcgaagta
    10921 gcgctcaagg tcagacaagt ccaagttttg aactgaggca ggcactacgt tgaaaaggaa
    10981 agccgcatct aggaagttgt catagtgaga aaagtcattt gatgggattt cagtgatgcc
    11041 tttttctttg acgatgttcc agtgtttagc acgcaagtct tttgcggctg caaggagttc
    11101 ttcttctgag atttcttttc taaagtattt ttcagttgta aattttaatt cacggaattc
    11161 gcccaaacga gggaaaccga tgattgtagt tgacatgatg tgtcctccaa aatttgttgt
    11221 tgaaactatc ttaacagaaa agaaagcgtc tgtataattg taaaaaatta ggctttgata
    11281 tagtttgaaa ctatatatct gtttcggaca aaagaaaaag acttgaagca aacgtctcaa
    11341 atcctttgta attcttactt tacagctata ttccaattag aatactaaaa catgttatta
    11401 gtaattctta taagtgacta tgacctgtta ttagaaaaga ctataactga ttctagtcaa
    11461 ctttttccct gttcaagtgg gacgattgct agtgtctttc ctaaactggc taggactttt
    11521 aagactgtat ccaactgagg actagtcttt cctgtctcca tcctagctat gacaggctgg
    11581 cttattccac tgacttcttc cagctttttc tgactgattc cttgttcata cctagcctca
    11641 atcaactcgc tcatgatagc cactcgcata tcactttcaa ggatttcctc cttgctaaag
    11701 agctcagatg gacatccttc caattactcc caatagcact attcttcatc acttaaccct
    11761 ctttctttta cgtctatgta tttaaaaaaa aatgagcgaa ttatgattcg atagattgac
    11821 cagcgggttt aaagttggtg ctagcctatt tcttaagcga ttttcctttt ctaggataaa
    11881 gcagatcctg cttgcttaac cccaattttc cacgatgaat ccaatagtaa atggttgaaa
    11941 ttcccacgtt aaccccttta gccatcacca tcatttcagg cgaaaatttt tggttatgta
    12001 tagtggagaa tcttttcctt tagttccttg gtcaagcttg atttcttgac cgagcgcttg
    12061 cgattgtttt cataagactg ttgagcgtag tcggcagaat aaacctcttt gaagcgccct
    12121 tttccaagac attttcggac tgtcccacgc ttgatttcag tgtggatagt ttgaggagct
    12181 tttccaagta gagaggcaac ttctctattt gattttcctt cttttttcca tctttcgatt
    12241 aagcgacggc tatcgattgt caaatgttta ccttttgtag tataattgtc ttgcatctct
    12301 gtgcctttta atcatttcaa tcttaaattg gacttttttt acttgggttg tacttaatct
    12361 ataaggaaga caagaaaaag aatatcaatc aagtaaagtc acaaagtcac attagctccg
    12421 agcaaccatt gcaaattgag gtactcacac aatgattaaa acatttctct ctgccctttc
    12481 ggtcattctc tttgttatcc ctatcataac ttattctttt tttccatctt ctaatcttaa
    12541 tatttggcta tctaccagac ctatcttggc acagatttat gccttcccct tagctactgc
    12601 aactatggct gccattttaa gtttcgtatt tttttcccta tctttttaca agaaaaataa
    12661 acaaatacgg ttttactctg gcattttgct cttactatcg ctcatattac tattattcgg
    12721 aacagataaa accctttctt ctgcatcaaa taagactaaa accttaaaat tagtaacttg
    12781 gaacgtcgct aatcaaatag aagcacaaca tattgagcga atttttagcc attttgatgc
    12841 cgatatggct atattccctg aactagctac caatatcaga ggtgagcaag aaaaccagag
    12901 aatcaaacta ttgtttcatc aagttggact ttctatggcc aactatgata ttttcacttc
    12961 tccacctacc aatagtggaa tagctcctgt gactgtgatt gtcaagaaaa gttatggttt
    13021 ctatacagaa gctaaaactt ttcatacaac acggttcggg acaattgtat tacattcgag
    13081 aaaacaaaat ataccagata tcattgcctt gcatactgct cctcctctgc caggtttaat
    13141 ggaaatctgg aagcaagact taaacatcat tcataatcaa ttggcttcaa aatatccaaa
    13201 ggctattatt gcaggtgatt ttaatgcaac tatgcgtcat ggagcacttg caaaaataag
    13261 ctctcatagg gacgcattga atgcacttcc accttttgaa agaggaactt ggaatagcca
    13321 aagtccaaaa ctttttaatg caacgataga tcatatctta ttgcctaaaa accactacta
    13381 tgttagagat ttagacattg taagttttca aaactctgat catagatgta tttttacaga
    13441 aatcacattt taattatttt atataaaatc acccctctag tgttcataaa ctagaggggg
    13501 aatttgtatc atactatcgt ttaacgcact tctgcattga ctttttcttc gagcgatgct
    13561 tggatttttt ccatatagcg tgcgacttct tcgtccgtta agctatcttc tggattttgg
    13621 aaggtcaagc tataagccat tgacttcata ccaagtccca atttctcacc tgagaagacg
    13681 tcaaagagtt tgatatctgt caaacgtttc acgccggcag cttggatagc atctacaact
    13741 tcttgatgag tcacttctgc cttgaggaga agggcaacgt cacggctgac tgccgggaat
    13801 ttggtgattt ctacaaatgg agtcgctggc tgaagcgcag cttcgatagc tgaaaggtta
    13861 agctcagcca catacgtttc tggaatatcg taagccttgg cagtgactgg atgcacttgg
    13921 ccaaggaaac caagaacttg gtcaccgaat gaaatcacgg ctgtacgacc tggatgaagg
    13981 ctagcgattt cagatgttgc tgtataggtt acttggagtc ccaaacgagt aaatagggct
    14041 tcaaggattc ccttagcata gaagaaatca actggaactg ctgctgtttg gaaatctttt
    14101 tcagcaacca agcctgtcaa ggcaaaggca aaactgttga tttcatttgg aagttcttct
    14161 tttggattac ctgtttgttc aaagactttt ccaatctcgt aaagggccaa gtttttattc
    14221 ttacgagcca cgttgtaggc aacggtatca aggatacctg aaatcatatt ttgacggagg
    14281 actgaacgat ccacagtcat tggccacatg agttccgtaa ggttacttgg ttgagccgta
    14341 aactcaactg ctttttcagg agttgttaga gtataggtga tgatttctgt caaacctgct
    14401 ccttcagcaa tagtacgaac ttgacggcgg agtttttgtg tggctgtcaa ttcacctgct
    14461 gtaccatcgt cttttggtag actagttggc aagcggtcat aaccatagat acgagcaatt
    14521 tcttcaaaga ggtcagcttc gattgtgata tcccaacgac gacgtgggac tctgactgta
    14581 aagctgtctg catttccaga aagaccaaag ccaagacgac ggaagacgtc ttctacatca
    14641 gcataagaca gctcagttcc gaggacacga ttaacatcag caagggttga agaaacttct
    14701 acatctgaag tatcaagctc acccgctgaa acgatgccct tacgcaccgt cgcacctgca
    14761 agttccgcaa tcaggctagc tgccgcatca agggcttcat taactgttgc cacattaatt
    14821 cctttttcaa agcgagaaga tgactcagaa cgaaggttca ggcgaccact tgtcttacgg
    14881 atagatttgc cattgaaaac agcagcttca aggacaacac gactagattt ttcagagatt
    14941 tctgttgctt gaccacccat gacacctgca agggctactg gcttgtctgc gacagtgatg
    15001 actaggtcat tcacgtccaa gtcacgttct tcaccgtcca aggtcaccaa tttttcacca
    15061 gcacgcgctt cacgcacacg gatgtcagtc ccttcaaagt tatccaagtc aaaggcatgc
    15121 attggttgac caaaatagag caggatgtag ttggtcacgt ccactacgtt attgatggga
    15181 cggattcctt cgttcatgag aagattttgc aaccattgtg gacttggtgc gatggtcaca
    15241 ttgtccaaga tacgagctgc atagtaaggc gccttgtctg tctcaatgct gacagaaagg
    15301 gcatctgccg cagcttcatt agtttctgtt agagtaaatt ctttaaagtt gactgccttg
    15361 tcatagatgg ctgccacttc gtgagccact ccacacatag aaagggcatc tgcacggttt
    15421 ggtgtgatgg aaagttcgat gatttcatca tccaagtcta ggtaagaaaa gacttcctca
    15481 cctggcacgg catcttcagg caagatttgg atgccatctg cgaattcctt aggcacaact
    15541 gagtcagaaa ttcccaattc accaagtgaa cagatcattc caagtgactc caaaccacgg
    15601 atttttcctt ttttgatttt gtagttatca gcgatacgag ctcctggaag agccaccatg
    15661 accttgatcc cagcacgcac atttggggca ccacaaacga tctgacgctc ttcttcgcca
    15721 acgttaacct gacaaacatg gaggtgagtc tctggcacat cttcgcaaga caagacctca
    15781 ccgacgacaa tttttgagag accagcagct ggtgattcga caccctctac ctcgattcct
    15841 gtagttgaca ttttttcagc caactcttgt gatggcacat caatgtccac caattctttt
    15901 aaccatttat aagatataag cataatttag ttctccagaa tgaccattgc cacactggtt
    15961 cttttccttt cctatcattt caatagaaga atcctcttct taccttaatt tctttctcag
    16021 taaccaatcc gtatctactt tttgaccaac cataaaatga tgttggctaa atttttcaaa
    16081 accatatcga ttataaaacg cttgagcttt tgtattatgc tcccaaacac ctagccaagc
    16141 ccaagaaaaa ctattttttg tagcaagttc aagtgcgaat tcaaacagtt gcttacctag
    16201 tccaaatcct tggaattttt gtagcacata gaggcgttga atttcaaaag cgtcctctaa
    16261 ttctctctca gtttgagcac ttccccagtt gactttgaga aaaccagcta tctcctcctc
    16321 atgcataatg aaataggttt cagagtcagg atttcccaac tcagttgaca aagttttcag
    16381 actataagcc tcttcaaagt attcctgtaa ctgctcttcc gtattatcat acgcaaaggt
    16441 ttcacgaaag gcttgtttgg caattttagc caacacctca acatctgcca tttctacttt
    16501 tctaatcatt atttaaactg ttctgagaag cggacatctc cttggtagaa tccacggata
    16561 tcgttgattc cataacggag catagctaca cgctcttgtc caagaccaaa ggcaaaacca
    16621 gagtatacag tcgcatcgat accactcatt tcaaggacac gtgggtgaac cataccggct
    16681 cccataattt cgatccaacc tgttttctta catacgttac agccttctcc accacacttg
    16741 aagcaagaaa catccacctc aacagatggc tctgtgaatg ggaagtaaga tggacgcaaa
    16801 cgaatttgac gctcttcacc aaacattttt tggacaatca actgaagcgt tccttgaaga
    16861 tcagccatag agatattttt cccaactacc aagccttcga tttggtggaa ttggtgactg
    16921 tgggtcgcat cgtccgtatc gcgacggaag acacgccctg gcgagatcat cttcaaagga
    16981 cctttagaaa aatcatgggc atccatagca cgcgcctgaa ctggagacgt gtgggtacgg
    17041 agcaagattt cttcagtgat atagaaagta tcctgcatat cacgagctgg gtggtctttt
    17101 ggaaggttca tacgttcaaa gttatagtag tcttgctcca cttcaaaacc atccacgact
    17161 tgataaccca taccgatgaa gatatcttcg atttcttcac tggtttgtgt caaaacgtga
    17221 cggtgaccag tcgcaactgg acgacctgga agcgtcacat cgatactctc gctagccagt
    17281 tgagccgcga ctttcttttc ttccaagagc ttagctgttt cttcaaaagc agctgtcaag
    17341 acatcacgag cttcattgac gtgtttcccg atgattggac gcatctcagc agaaacatct
    17401 ttcatccctt tgaggatttc agtgagcgaa ccctttttac caaggacaga gacacgcaaa
    17461 tcttgcatct ctttttcatt tccagcagta atctgcttca agctagtcag cgtttcttcg
    17521 cgaagcgctt ttaattgttc ttcaatagtt gacatatttc ctccatcagt ctctcgtaga
    17581 taaaaagaaa accacatgcc aaaaactcca ctcggagcgt tgacacgcgg taccatccgt
    17641 tttcatctga caagtcagac cttcatttct aaatccatgc gcaagtgaat tcacccagct
    17701 ttcatataga gagcttgcag tcacggctct cctccctgat atacttccct tgggctacta
    17761 gtctttcaga tttctattca attaccactt agtttatcag atttttacca ttcttgcaag
    17821 acctatctta cttctgcttg ttagcttatt cttatctaac tttatagcgg attgaaataa
    17881 gatga
//
LOCUS       DAVMEI010000025        17497 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVMEI010000025 DAVMEI010000000
VERSION     DAVMEI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.025"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            37..1188
                     /gene="nagA"
                     /locus_tag="VYQ24_000674"
     CDS             37..1188
                     /gene="nagA"
                     /locus_tag="VYQ24_000674"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1967845.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            1351..2409
                     /locus_tag="VYQ24_000675"
     CDS             1351..2409
                     /locus_tag="VYQ24_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1967846.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            2560..2925
                     /locus_tag="VYQ24_000676"
     CDS             2560..2925
                     /locus_tag="VYQ24_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1967847.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            3000..3941
                     /locus_tag="VYQ24_000677"
     CDS             3000..3941
                     /locus_tag="VYQ24_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1967848.1"
                     /translation="MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            3889..4905
                     /locus_tag="VYQ24_000678"
     CDS             3889..4905
                     /locus_tag="VYQ24_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1967849.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            4907..5233
                     /locus_tag="VYQ24_000679"
     CDS             4907..5233
                     /locus_tag="VYQ24_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1967850.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVSDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            5226..5630
                     /locus_tag="VYQ24_000680"
     CDS             5226..5630
                     /locus_tag="VYQ24_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1967851.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            5635..5895
                     /locus_tag="VYQ24_000681"
     CDS             5635..5895
                     /locus_tag="VYQ24_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967852.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            5858..6319
                     /locus_tag="VYQ24_000682"
     CDS             5858..6319
                     /locus_tag="VYQ24_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1967853.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            6297..6743
                     /locus_tag="VYQ24_000683"
     CDS             6297..6743
                     /locus_tag="VYQ24_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1967854.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            6846..7433
                     /locus_tag="VYQ24_000684"
     CDS             6846..7433
                     /locus_tag="VYQ24_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1967855.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKVILDFG
                     GGTGLLALPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLIVAQKSLA"
     gene            7494..8447
                     /locus_tag="VYQ24_000685"
     CDS             7494..8447
                     /locus_tag="VYQ24_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1967856.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSNLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            8498..9688
                     /locus_tag="VYQ24_000686"
     CDS             8498..9688
                     /locus_tag="VYQ24_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1967857.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            9837..10208
                     /gene="rnpA"
                     /locus_tag="VYQ24_000687"
     CDS             9837..10208
                     /gene="rnpA"
                     /locus_tag="VYQ24_000687"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1967858.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVN"
     gene            10183..11007
                     /locus_tag="VYQ24_000688"
     CDS             10183..11007
                     /locus_tag="VYQ24_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1967859.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            11026..12012
                     /locus_tag="VYQ24_000689"
     CDS             11026..12012
                     /locus_tag="VYQ24_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1967860.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVNAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            12063..12686
                     /locus_tag="VYQ24_000690"
     CDS             12063..12686
                     /locus_tag="VYQ24_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1967861.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            12817..14274
                     /locus_tag="VYQ24_000691"
     CDS             12817..14274
                     /locus_tag="VYQ24_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1967862.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLAAYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            14297..14578
                     /locus_tag="VYQ24_000692"
     CDS             14297..14578
                     /locus_tag="VYQ24_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1967863.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQRDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            14654..15139
                     /locus_tag="VYQ24_000693"
     CDS             14654..15139
                     /locus_tag="VYQ24_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1967864.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            15156..15821
                     /locus_tag="VYQ24_000694"
     CDS             15156..15821
                     /locus_tag="VYQ24_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1967865.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            15825..16688
                     /locus_tag="VYQ24_000695"
     CDS             15825..16688
                     /locus_tag="VYQ24_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1967866.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            16690..17394
                     /locus_tag="VYQ24_000696"
     CDS             16690..17394
                     /locus_tag="VYQ24_000696"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1967867.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYRAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
BASE COUNT         5384 a         3092 c         4015 g         5006 t
ORIGIN      
        1 aaattgtaga atcaatcttt taaggaggtt aacattatgc ctaactatat taaagcggat
       61 cagtttttct acccacacgg agttcgtcga ggtggttact tggaacttgt ggacggcaag
      121 tttgggaaac atgtagagca gattcctgaa ggggctgagg tgattgacta tacaggttat
      181 agcattgccc caggtcttgt ggatactcat attcatggat atgcaggtgt agatgtgatg
      241 gacaacaaca ttgaaggtac attgcatact atgagtgaag gacttcttag taccggtgtt
      301 accagtttct tacccacaac tttaacagcc acttatgagc aattgcttgc agtcactgaa
      361 aatcttggaa accattataa agaagcaaca ggtgctaaga ttcgtgggat ttattatgaa
      421 ggtccatatt tcacagaaac ttttaagggg gcacaaaatc caacttatat gagagacccg
      481 ggtgttgagg agtttcattc ttggcaaaaa gcggcaaatg gcttgcttaa taaaattgcc
      541 cttgcaccag aacgtgatgg ggtggaagac tttgttcgta cagttacggg cgaaggtgtg
      601 acggttgctc ttggacattc aaacgcgact tttgatgaag ccaaaaaagc agtcgatgct
      661 ggagcgagtg tttgggtgca tgcctacaat ggaatgcgtg ggttgactca ccgtgaattg
      721 ggtatggttg gagccatgta ccaattgcca catacctatg cagagttgat ctgtgatggt
      781 caccacgtag atccaaaggc ttgcgaaatt cttatcaaac aaaaaggaac agaaaacatc
      841 gctcttatca cagactgtat gacagctggg ggattggaag acggagatta tatgttggga
      901 gaattcccag ttgtcgttgc aaatggaact gcacgcctca aatcgacagg taacttggca
      961 ggttctatcc tcaaactcaa agatggtttg aagaatgtgg tcgaatgggg aattgcgaat
     1021 ccgcatgaag cagtcatgat ggccagcttc aacccagcta aatccgttca catcgatgac
     1081 gtctgtggcc aaatccgtga aggctacgac gctgacttca tcgtattaga taaagatttg
     1141 gaattggtag caacctatct agatggcgta aaacgttatc aagcataaga gagaaggcag
     1201 aagttagaga ctagcttctg cttttttata cagggtactt tactggtaaa taaaaagtta
     1261 aaaaaatcac aaaaaaagct tgaagaaaca aagaaaaagg cttacaattg aactataaat
     1321 attacaaata aaaaaacgga ggagtgcttt atgaaagcct atacttatgt taaaccagga
     1381 cttgcttctt ttgttgatgt agacaaacca gttattcgca agccaacaga cgctattgtg
     1441 cgtattgtaa aaaccactat ttgtggaaca gacctccata ttatcaaagg ggatgttcct
     1501 acttgccaaa gtggtaccat tcttggccac gaagggattg ggattgttga agaagttggg
     1561 gaaggagttt ccaacttcaa aaaaggcgac aaggtcttga tttcttgcgt ctgtgcctgt
     1621 ggtaaatgct actactgtaa aaaaggaatt tatgcccact gtgaagacga agggggctgg
     1681 attttcggtc acttgattga tggtatgcag gctgaatatc tacgtgtccc tcatgcagat
     1741 aatactcttt accatactcc agaagacttg tcagatgaag ctttggttat gctgtcagac
     1801 attctgccta ctggatatga aattggtgtc ttaaaaggga aagtagaacc tggttgcagc
     1861 gtagccatta ttggttcagg tccagttgga ttggctgctc ttttaacagc ccaattctat
     1921 tcaccagcta aattgattat ggtagaccta gacgataacc gcttggaaac tgccctatca
     1981 ttcggtgcga ctcataaggt taattcttca gaccctaaaa aagccattaa agaaatttat
     2041 gatttgacag atggtcgtgg tgtggatgtc gctatcgaag ctgtagggat tcctgaaaca
     2101 tttgatttct gtcaaaagat tatcggtgta gacggaacgg ttgccaactg tggtgtgcat
     2161 ggtaaaccag ttgaattcga tttagataaa ctttggattc gcaacatcaa tgtaacaact
     2221 ggtttggtat ctacaaatac gactccacaa ttgttgaaag cacttgaaag tcataagatt
     2281 gaaccggaaa aattggtaac tcactatttc aaactcagtg aaattgaaaa agcctacgaa
     2341 gtcttcagta aggcagcaga ccaccatgcc attaaggtca ttatcgaaaa cgatatctca
     2401 gaagcctaag tagtaaaaat atttttgtac ataagtaaat agaaattcag tcatccatca
     2461 gatggttgga ttttttatca aaaaattaag aaatgagcat atttctttcc ttgtctggcg
     2521 gaattggtta taatatacgg tacaaaggaa tgaatgaata tgtatcgtgt tatagaaatg
     2581 tacggagatt ttgaaccgtg gtggttctta gaaggttggg aagaagatat tgtagcaagt
     2641 agaaaatttg accagtatta tgatgctctc aaatactaca aaacttgctg gtttagattg
     2701 gaacaagaat cgcctcttta taaaagtaga agcgacttga tgaccatttt ttgggacccg
     2761 gaagaccaac gctggtgtga tgaatgtgat gagtatttac aacaatacca ttctttggct
     2821 cttttgcagg atgagcaggt tatcccagac gaaaaactac gctcaggcta tgaaaaacaa
     2881 accagtcagg aaaggaatcg ttcttgccgt atgaaattaa aatagagaaa agtaactttt
     2941 tggagttgct ttttttattt ttctaacgct ttgcgaatag tataggtgag gaggtaagta
     3001 tggttcaaga aattgcacaa gaaatcattc gttcggctcg gaaaaaaggg gcgcaagaca
     3061 tttattttgt ccccaagtta gatgcctatg agcttcatat gagggtagga gacgagcgct
     3121 gtaaaattgg tagctatgat tttgaaaagt ttgcagccgt tatcagtcac tttaagtttg
     3181 tggcgggtat gaatgtggga gaaaaaagac gtagtcaact gggttcctgt gattatgcct
     3241 atgaccagaa gatggcgtct ctacgtttat ctactgtagg cgattatcgg gggcatgaga
     3301 gtttggttat ccgtttgttg cacgatgagg agcaggactt gcatttttgg tttcaggata
     3361 ttgaagaatt aggcaagcag tacaggcaac ggggactcta tctttttgct ggtccggttg
     3421 ggagtggtaa gacgaccttg atgcatgaat tgtccaagtc actctttaaa ggacagcaag
     3481 ttatgtccat cgaagatcct gtcgaaatca agcaggacga catgcttcag ttgcagttga
     3541 acgaagcaat cggcctaacc tatgaaaatc taatcaaact ttccttgcgt catcgaccag
     3601 atctcttgat tatcggagaa attcgtgaca gcgagacggc gcgtgcagtg gtcagagcta
     3661 gtttgacagg tgcgacagtc ttttcaacca ttcacgccaa gagtatccga ggtgtttatg
     3721 agcgtctgct ggagttgggt gtgagtgaag aagaattggc agttgttctg caaggagtct
     3781 gctaccagag attaatcggg ggaggaggaa tcgttgactt tgcaaacaga gattatcaag
     3841 aacaccaagc agccaagtgg aatgagcaaa ttgaccagct tcttaaagat ggacatatca
     3901 caagtcttca ggctgagacg gaaaaaatta gctacagcta agcaaaaaaa tatcatcacc
     3961 ctatttaaca atctcttttc tagcggtttt catctggtgg agactatctc ctttttagat
     4021 aggagttcct tgttggacaa gcagtgtgtg acccagatgc gtacaggctt gtctcagggg
     4081 aaatcattct cagaaatgat ggaaagtttg ggatgttcaa gtaccattgt cactcagtta
     4141 tctctagctg aagttcatgg caatcttcac ctgagtttgg gaaagataga agaatatcta
     4201 gacaatctgg ccaaggtcaa gaaaaagtta attgaagtag cgacctatcc tttgattttg
     4261 ctgggttttc ttctcttaat tatgctgggg ctacggaatt acctgctccc acaactggat
     4321 agtagcaata ttgccacccg aattatcggt aatctgccac aaatttttct aggcatggta
     4381 gggcttgttt ccgtgcttgc ccttttagca ctaacttttt ataaaagaag ttctaagatg
     4441 agtgtctttt ctatcttagc acgccttccc tttattggaa tctttgtgca gacctacttg
     4501 acagcctatt atgcacgtga atgggggaat atgatttcac agggaatgga gctgacgcag
     4561 atttttcaaa tgatgcagga acaaggttcc cagctcttta aagaaatcgg tcaagatctg
     4621 gctcaaaccc tgaaaaatgg ccgtgaattt tctcagacga taggaaccta tcctttcttt
     4681 aggaaggaat tgagtctcat catagagtat ggggaagtta agtccaagct gggtagtgag
     4741 ttggaaatct atgctgaaaa aacttgggaa gcctttttta cccgagtcaa ccgcaccatg
     4801 aatttggtgc agccactggt ttttatcttt gtggcactga ttatcgtttt actttatgcg
     4861 gcaatgctca tgcccatgta tcaaaatatg gaggtaaatt tttaaaatga aaaaaatgat
     4921 gacattcttg aaaaaagcta aggttaaagc ttttacattg gtggagatgt tagtagtctt
     4981 gctgattatc agcgtgcttt tcttgctctt tgtacctaat ctgaccaagc aaaaagaagc
     5041 agtcagtgac aaaggaaaag cagctgttgt taaggtggtg gaaagccagg cagaacttta
     5101 tagcttggat aaaaatgaag atgctactct gagcaagtta caaggagatg ggcgcatcac
     5161 ggaagaacag gttaaagctt ataatgaata ctatactaaa aatggagggg caaatcgtaa
     5221 agtcaatgat taaggccttt accatgctgg aaagtctctt ggttttggga cttgtgagta
     5281 tccttgcctt gggcttatcc agctctgtcc agtccacttt tgcagcagta gaggagcaga
     5341 ttttctttat ggagtttgaa gaactctatc gggaaaccca aaaacgcagt gtagccagtc
     5401 agcaaaagac tagtctaaat ttagatgggc agacgcttag caatggcagt caaaagttga
     5461 cagttcctaa aggaattcag gcaccatcag gccaaagtat tacatttgac cgagctgggg
     5521 gcaattcgtc cctggctaag gttgaatttc agaccagtaa aggagcgatt cgctatcaat
     5581 tatatctagg aaatggaaaa attaaacgca ttaaggaaac aaaaaattag ggcagtgatt
     5641 ttactggaag cagtagtcgc tctagctatc tttgccagca ttgcgaccct ccttttggga
     5701 caaattcaaa aaaataggca agaggaagca aaaatcttgc aaaaggaaga agtcttgagg
     5761 gtagctaaga tggccctgca gacggggcaa aatcaggtaa gcatcaacgg agttgagatt
     5821 caggtatttt ctagtgaaaa aggattggag gtctaccatg gttcagaaca gttgttggca
     5881 atcaaagagc cataaggtca aggcttttac cttgttagaa tccctgcttg ccctcattgt
     5941 catcagtggg ggattactcc tttttcaaac tatgagtcag ctcctcattt cagaagttcg
     6001 ctaccagcaa caaagcgagc aaaaggagtg gctcttgttt gtggaccaac ttgaggcaga
     6061 attagaccgt tcgcagttcg aaaaagtaga aggcaatcgc ctatacatga agcaagatgg
     6121 caaggacatc gccatcggta agtcaaagtc agacgatttt cgtaaaaccg atgctagtgg
     6181 acgggggtat cagcctatgg tttatggcct caaatcagct cagattacag aggacaatca
     6241 actggttcat tttcgtttcc agtttcaaaa aggcttagaa agggagttca tctatcgtgt
     6301 ggaaaaagaa gaaagttaag gcaggtgttc tcctctacgc agtcaccata gcagccatct
     6361 ttagtctttt gttgcaattt tatttgaacc gacaaatcgc ccactatcaa gactatgctt
     6421 taaataaaga aaaattggtt gcttttgcca tggctaaacg aaccaaggat aaagccgagc
     6481 aagaaagtgg ggaacaggtt tttaatctag gtcaggtaag ctatcaaaac aagaaaacaa
     6541 gcttggtgac gactgttcgt acgtctaaga gccaatatga gtttctgttt ccttcagtca
     6601 aaatcaaaga agagaaaaga gataaaaagg aagaggtagc gaccgattca agcgaaaaag
     6661 cggagaaaaa aaatcagaag agaagcctga aaagaaagag aattcctagt caattcaact
     6721 ataatgcgtt gaatccagaa tagtccactg tagtttctag aaaattgctg gaaatggatg
     6781 ttaagctcca attcatttgt ttatatctta tttcagttta ctatactttg tgctaaacta
     6841 aagatatgaa acatgatttt aaccacaaag cagaaacttt cgactcccct aaaaatatct
     6901 tcctcgcaaa cttggtatgt caagcagtcg agaaacagat tgatatttta tcagacaaag
     6961 taattttaga tttcggtggt ggcacgggtc tattagcctt gcccctagcc aagcaggcta
     7021 agtcagttac tcttgtagac atttctgaga aaatgttgga gcaagctcgt ttgaaagtgg
     7081 agcagcaagc aatcaagaat atccagtttt tggagcaaga tttaccgaaa aatcccttgg
     7141 agaaagagtt tgattgcctt gctgttagtc gggttcttca tcatatgcct gatttggatg
     7201 cggctctctc actgtttcat caacatttga aggaagatgg gaaactcatc attgctgatt
     7261 ttaccaagac agaagctaat catcatggat ttgatttagc tgaactggaa aacaagctaa
     7321 ttgagcatgg tttttcatct gtgcatagtc agattctcta tagcgctgaa gacctgtttc
     7381 aaggaaatca ctcagaattc tttttaatag tagcccaaaa atcactcgcc tagtcaggga
     7441 gtgatttttc tataaggatg gaaaaaagaa gggaaatttg ataagatagg aatatggatt
     7501 ttgaaaaaat tgaacaagct tatacctatt tactagagaa tgtccaagtt atccaaagtg
     7561 atttggcgac caacttttat gacgccttgg tggagcaaaa cagcatctat ctggatggtg
     7621 aaactgagct aaaccaggtc aaagacaaca atcaggccct taagcgttta gcactacgca
     7681 aagaagaatg gctcaagacc taccagtttc tcttgatgaa ggctgggcaa acagaaccct
     7741 tgcaggccaa tcaccagttt acaccagatg ccattgcttt gcttttggtg tttattgtgg
     7801 aagagttgtt taaagaggag gaaattacta tcctcgaaat gggttctggg atgggaattc
     7861 taggcgctac tttcttgacc tcgcttgata aaaaggtgga ttacttggga atggaaatgg
     7921 atgatttgct gattgatctg gcagctagca tggcagatgt aattggtttg caggctggct
     7981 ttgtccaagg agatgccgtt cgtccacaaa tgctcaaaga aagcgatgtg gtcatcagtg
     8041 acttgcctgt tggctattat cctgatgatg ccgttgcgtc gcgccatcaa gttgcttcta
     8101 gccaagaaca tacttacgcc catcacttgc tcatggaaca agggcttaag tacctcaagt
     8161 cagacggata cgctattttt ctagctccga gtaatttgtt gaccagtcct caaagtgatt
     8221 tgttaaaaga atggctgaaa gaagaggcga gtctggttgc tatgattagt ctgcctgaaa
     8281 atctctttgc taatgccaaa caatctaaga ctatttttat cttacagaag aaaaatgaaa
     8341 tagcagtaga gccttttgtt tatccacttg ctagcttgca agatgcaagt gttttaatga
     8401 aatttaaaga aaattttcaa aaatggactc aaggtactga aatataaaat agattttgtt
     8461 ataatagttg aaaacgctta aaaaggggta tcatgttatg acaaaaacaa ttgcaatcaa
     8521 tgcaggaagt tcaagtttga aatggcaatt atacttaatg ccagaagaaa aagtgttagc
     8581 caaaggtttg attgaacgta tcggtttgaa agattcaatt tcaactgtaa aatttgacgg
     8641 ccgttctgag caacaaattt tggatattga aaatcataca caagccgtta aaattttatt
     8701 ggatgacttg attcgtttcg atattatcaa ggcttatgac gagattacag gtgttggaca
     8761 tcgtgttgtt gctggtggag aatatttcaa agaatcaaca gttgttgagg gagatgtttt
     8821 agaaaaagtt gaagagttga gtttgttggc tcctctacac aacccggcca atgcagcagg
     8881 tgttcgtgcc ttcaaggaat tgttgccaga cattaccagt gtagttgttt ttgatacttc
     8941 cttccacaca agtatgccag agaaagctta tcgctaccct ctaccaacaa aatattacac
     9001 agaaaacaag gttcgtaaat acggtgctca tggtacaagt caccagtttg tagcaggaga
     9061 agctgcaaaa ctcttgggac gtccattaga agacttgaag ttaattacct gtcatattgg
     9121 taacggaggc tcaattacag ctgtgaaagc cggcaaatct gtagacactt ctatggggtt
     9181 cactcctctt ggtggtatta tgatgggaac gcgtacaggg gatattgatc cagctatcat
     9241 tccttattta atgcaatata cagaggattt taacacacca gaagatatca gtcgtgttct
     9301 taaccgtgaa tcaggtcttt tgggagtttc tgctaattct agcgatatgc gcgatataga
     9361 agcagctgta gcagaaggga atcacgaggc tagcttggct tatgaaatgt atgttgaccg
     9421 tatccaaaaa catatcggtc agtaccttgc agtgctaaat ggagcagatg ccattgtttt
     9481 cacagcaggt gtcggtgaaa atgcagagaa tttccgtcgt gatgtaatct cagggatttc
     9541 gtggtttggt tgtgatgttg atgatgaaaa gaatgtcttt ggcgttacag gagacatctc
     9601 aacagaggca gctaaaatcc gtgtcttggt tattccaaca gatgaagaat tagtcattgc
     9661 ccgtgacgtt gaacgcttga aaaaataagt gaaactaaaa aaatattcaa tacaaggagt
     9721 tgggaaagtt atttttccag cttctttttc tgatgaaatt gtccaaaacc ttgctatgat
     9781 tggctttttt gaaaaatatg gtataatagt agtaatttaa tagatggagt tgagttttga
     9841 agaaaaactt tcgtgtaaaa agagagaaag attttaaggc gattttcaag gaggggacaa
     9901 gttttgctaa tcgcaaattt gtggtctacc aattagaaaa ccagaaaaac cattttcgag
     9961 taggtctatc agttagcaaa aaactgggga atgccgtcac tagaaatcaa attaagcgac
    10021 ggattcggca tattatccag aatgcaaaag ggagtctggt agaagatgtc gactttgttg
    10081 tcattgctcg aaaaggagtc gaaaccttgg gatacgcaga gatggagaaa aatctactcc
    10141 atgtattaaa attatcaaag atttaccggg aaggaaatgg gagtgaaaaa gaaactaaag
    10201 ttaactagtt tgctaggact gtctctgtta atcatgacag cctgtgcgac taatggggta
    10261 actagcgata ttacagccga atcggctgat ttttggagta aattggttta cttctttgcg
    10321 gaaatcattc gctttttatc gtttgatatt agtatcggag tggggattat tctctttacg
    10381 gtcttgattc gtacagtcct cttgccagtc tttcaggtgc aaatggtggc ttctaggaaa
    10441 atgcaggaag ctcagccacg cattaaggcg cttcgagaac aatatccagg tcgagatatg
    10501 gaaagcagaa ccaaactaga gcaggaaatg cgtaaagtat ttaaagaaat gggtgtcaga
    10561 cagtcagact ctctttggcc gattttgatt cagatgccgg ttattttggc cctgttccaa
    10621 gccctatcaa gagttgactt tttaaagaca ggtcatttct tatggattaa ccttggtagt
    10681 gtggatacaa cccttgttct tccgatttta gcagcagtat tcaccttttt aagtacttgg
    10741 ttgtccaaca aagctttgtc tgagcgaaat ggcgctacga ctgcgatgat gtatgggatt
    10801 ccagtcttga tttttatctt tgcagtttat gcgccaggtg gagtcgccct atactggaca
    10861 gtgtctaatg cttatcaagt cttgcaaacc tatttcttga ataatccatt caagattatc
    10921 gcagagcgcg aggccgtagt acaggcacaa aaagatttgg aaaatagaaa aagaaaagcc
    10981 aagaaaaagg ctcagaaaac gaaataaata aggaggaatc tggtaatggt agtatttaca
    11041 ggttcaactg ttgaagaagc aatccagaaa ggattgaaag aattagatat tccaagaatg
    11101 aaggctcata tcaaagtcat ttctagggag aaaaaaggct ttcttggtct atttggtaaa
    11161 aaaccagccc aagtggatat tgaagcgatt agtgaaacga ctgttgtcaa agcaaatcaa
    11221 caggtagtaa aaggcgttcc gaaaaaaatc aatgatttga acgagcctgt gaagacggtt
    11281 agtgaagaaa ccgttgacct tggtcatgtg gttaatgcta ttaaaaaaat agaggaagaa
    11341 ggtcaaggta tttctgatga agtcaaggct gaaatcttaa aacatgaaag acatgccagc
    11401 actatcttag aagaaactgg tcacattgag attttaaatg aacttcaaat cgaggaagcg
    11461 atgagggaag aagcaggcgc tgatgacctt gaaactgagc aagatcaaac tgaaaatcaa
    11521 gacttgaaag agatgggctt gaaggtcgag caaagttatg atattgccca ggtggctacg
    11581 gatgtgactg cctatgttca agcgattgtg gatgacatgg atgttgaggc tacactttca
    11641 aatgattata accgtcgtag catcaatcta caaattgaca ccaacgaacc aggtcgtatt
    11701 atcggctacc atggtaaagt cttgaaggcc ttgcaactgt tggctcaaaa ttatctttac
    11761 aaccgctatt ccaaaacctt ctacgttaca atcaatgtca atgattatgt cgaacaccgt
    11821 gcagaagtct tgcagaccta tgcgcaaaaa ttggcgaatc gtgttttgga agaaggtcgc
    11881 agtcataaaa cagatccaat gtcaaatagc gaacgcaaga ttatccatcg tattatttca
    11941 cgtatggatg gcgtgactag ttactctgaa ggtgatgagc caaatcgcta tgttgttgta
    12001 gatacagaat aagtaaaatc agggttgacc tgattttttg ctagttagag gaggttaaac
    12061 tgatgttgaa taagataaga gactatctag acttttctgg tttgcagtac cgtagtcctg
    12121 ataaagcggg agcagagcga gagaagatgc tggcgttccg ccacaaagga caagaggccc
    12181 gaaaggcttt tacagaactg gccaaagcct ttcaagcaag ccatccagaa tggcaactcc
    12241 aacagactag tcagtggatg aatcaggccc agcgtttgag accacatttt tgggtttatc
    12301 tacagagaga cggacaagtg acagaaccta tgatggcctt acgtttgtat gggacatcta
    12361 ctgactttgg aatttctttg gaagtcagtt tcatcgaacg taagaaggat gagcaaacac
    12421 tgggcaagca ggccaaagtt ttagacattc caaccgttaa agggatttat tatctaacct
    12481 actctaatgg tcaaagtcaa cggtgggagg cgaatgaaga aaagcgtcgt actttacgcg
    12541 agaaggtgag aagtcaagaa gttcgaaaag ttttagtgaa ggtagatgtt cctatgacag
    12601 aaaattcgtc tgaagaagaa atcgtagaag gcttattgaa gtcttattct aaaattcttc
    12661 cctattatct agctacgaga aaataagata atttgtaaaa catcataaat catacagtcc
    12721 aagagtgaac agtccgctgt gtaattcttg gtctttttgt ttgcgctttc gcattatata
    12781 ataaacttac aaaaacaatt caaaaggaga acaattatgg aagtcgtttc aagtgttcta
    12841 aattggtttt ctagcaatat tttgcagaat cccgcatttt tcgtaggttt attggtgttg
    12901 ataggatatg cacttttgaa aaaacctgcc catgacgttt tttcagggtt tgttaaagca
    12961 acagtagggt atatgttgct taacgtgggt gctggtggtt tggttacaac ctttcgtcca
    13021 atcttagcag ctcttaacta caaattccaa attggtgcag cggttatcga cccttatttt
    13081 ggacttgctg cagcaaacaa caaaattgta gcagagtttc cagattttgt tggaactgca
    13141 actacagctc tattgattgg ttttggaata aatatcttgc tcgtagctct tcgaaagatt
    13201 acgaaggtaa gaaccctctt tattactggt cacatcatgg tacaacaagc tgcaacagta
    13261 tctcttatgg ttctattctt agtaccacaa ttgcgcaatg cttacggtac agcagcgatt
    13321 ggtatcatct gtggacttta ctgggcagtt agttcaaata tgactgttga ggcaactcaa
    13381 cgcttgactg gtggtggcgg atttgcgatt ggtcaccaac agcaatttgc aatctggttt
    13441 gtagataaag tagcaggacg ctttggtaag aaagaagaaa gtttagacaa tcttaaatta
    13501 cctaagttcc tctcaatctt ccacgataca gttgttgcat ctgctacctt gatgctcgta
    13561 ttcttcggag ccattctttt aatcttgggt ccagacatta tgtctaataa agaagtcatc
    13621 acttcaggaa ctctattcaa tcctgctaaa caagatttct ttatgtacat tatccaaaca
    13681 gcctttacct tctcagttta cttgttcgtt ttgatgcaag gtgtccgaat gttcgtatct
    13741 gagttgacaa acgccttcca aggtatttca aacaaattgt tgccaggttc attcccagcg
    13801 gttgacgttg cagcttctta tggatttggt tctccaaatg ctgtcttgtc aggatttacc
    13861 tttggtttga ttggtcaatt gattacaatt gtcttgctca tcgtctttaa aaatccgatt
    13921 cttattatta caggatttgt accagtgttc tttgacaatg cagccattgc ggtctacgct
    13981 gataaacgcg gcggatggaa agcggctgtt atcctttcct ttatatcagg tgtccttcaa
    14041 gttgctctag gagctctttg tgtggccctt ctcgatttgg cagcttatgg tggctaccat
    14101 ggaaatatcg actttgaatt cccatggctt ggatttggat atatcttcaa ataccttggt
    14161 attgttggtt atgtacttgt gtgtctcttc ttgcttgtta ttcctcaact tcaatttgcc
    14221 aaagcaaaag ataaagagaa atattacaac ggtgaagttc aagaagaagc ttagtatcta
    14281 gaaaaggaga aataaaatgg ttaaagtatt agcagcgtgc ggaaatggaa tgggttcatc
    14341 aatggttatc aagatgaagg ttgaaaatgc tctccgtaag cttaatcaaa gagattttac
    14401 agtcaattca tgcagtgtcg gtgaagctaa aggtttagca gtaggatatg acatcgtaat
    14461 cgcttctctt catttgattc aagaattgga agggcgaact aatgggaagt taattgggct
    14521 tgataacttg atggatgata aagaaatcac cgaaaaactc agtcaagcac tacagtaaaa
    14581 ggttggaggg ggctggacag aaactgagag ttatcgtttc tgtccttctc cctcttaaat
    14641 aaaggaggca gatatgaatt taaaacaagc tttaattgac aatgactcga tccgactagg
    14701 tttagaggct aacaattgga aagaagcagt caaggtagca gtagatccct taattgaaag
    14761 tggggcaatt ttgccagagt attacgatgc tatcattgaa tcgactgaag agtatgggcc
    14821 ttactatatc ttgatgccag gtatggctat gccccacgct agacctgaag ctggtgtgca
    14881 aagtgatgcc ttttcattga ttaccttaca aaatcctgtt gtattttcag atgggaaaga
    14941 ggtatctgtt ttgttggcac tagcagcaac aagctcaaaa attcacacaa gtgtagccat
    15001 tccacaaatt attgccctgt ttgaattaga agattctatt gcacgtttac aggcttgcca
    15061 gactaaagaa gatgtcttgg ctatgattga agaatctaag gatagccctt atctcgaagg
    15121 attggatttg gaaagttaga aagaggaata aagaaatgac aaaaagaata cctaatttac
    15181 aagttgcatt agaccattca gacttgcaag gagcgattaa agcagctgtt tctgttggtc
    15241 aggaagtaga tattatcgaa gctggaactg tttgcttgct tcaagttgga agtgaactgg
    15301 ctgaagtctt gcgtagcctt ttcccagata agattattgt ggcagacaca aaatgtgctg
    15361 atgctggtgg aacagttgct aaaaataatg cggttcgtgg agcagactgg atgacttgta
    15421 tctgttgtgc aaccatccct actatggaag cagctctaaa ggctatcaag actgaacgag
    15481 gagaacgagg cgaaatccag atcgagcttt atggcgattg gacttttgaa caagctcagc
    15541 tttggctaga tgcaggtatc tcacaagcta tttatcacca atctcgtgat gctcttcttg
    15601 ctggtgaaac ttggggtgaa aaagacctta ataaggttaa aaaactcatt gacatgggct
    15661 tccgtgtatc tgtaacaggt ggtctagatg tagatactct caaactcttt gaaggtgttg
    15721 atgtctttac ctttatcgca ggtcgtggaa ttacagaggc tgcggatcca gcaggagcag
    15781 cgcgtgcctt caaggatgaa atcaaacgaa tttgggggta aatcatggta cgtccaattg
    15841 gaatttatga aaaggcaacc ccaacacact ttacttggct agaacgttta aattttgcca
    15901 aggagttagg ctttgatttt gtcgagatgt ctattgacga acgtgacgag cgtttagcaa
    15961 gacttgactg gagtaaggaa gaacgcttgg aagttgtcaa agcaatctat gaaactggtg
    16021 ttcgtattcc ttctatctgt ttttcaggcc atcgtcgcta cccattgggt tcaaaagatc
    16081 cagttctaga ggaaaaatct ctagaactca tgaaaaaatg tatcgaatta gctcaagact
    16141 tgggagttcg tacgattcaa ttagctggtt acgatgttta ctatgaggaa aagtcacccc
    16201 agacacgcca acgttttatc aaaaatttga gaaaagcctg tgactgggct gaagaagctc
    16261 aggtggtact tgctattgaa attatggatg atcctttcat cagtagcatc gaaaaatatt
    16321 tggctataga aaaagagatt gactctccct tcctctttgt atatccagat attggtaatg
    16381 tgtctgcatg gcataatgat atctatagtg agttttatct tggtcatcat gccatcgcag
    16441 ctctccatct caaggatact tatgcagtga cagaaagttc aaggggccag ttccgagatg
    16501 taccttttgg gcaaggttgt gtcaaatggg aagaagcttt cgatatttta aaggaaacca
    16561 attataatgg acctttccta atcgaaatgt ggtctgaaaa ttgtgaaaca gtagaagaaa
    16621 cacgcgcagc cattcaagag gcgcaagctt ttctctatcc actcattaag aaagcaggtt
    16681 tgatgtaaga tgaatcaagt aatcaatgct atgcgtaaac gagtctgtga tgccaatcaa
    16741 tcattgccaa aacatggact tgtcaaattt acctggggga atgtatctga agtcaatcgc
    16801 gaactcggtg tcattgttat caaaccatca ggcgtggatt atgacgaatt gacacctgaa
    16861 aacatggtag tgactgatct agatggtaag atcctagaag gggatttaag accatcttcc
    16921 gacctcccaa ctcatgtgca attatatagg gcttggtcag aaattggtag tgtggttcac
    16981 acccattcga cagaagctgt tggttgggct caggcaggtc gtgatattcc tttctacgga
    17041 acaacccatg cagattattt ctacggttca atcccttgcg cccgtagttt gaccaaggac
    17101 gaagtagaag tggcctatga aaaagatact ggcctggtta tcgtagaaga atttgaacat
    17161 cgcggactta acccggttga agtaccagga attgttgtac gcaatcacgg tccattcacc
    17221 tggggcaaaa atccagagaa tgctgtttat cactctgtcg tactagagga agtatcaaag
    17281 atgaatcgct ttacagaaca aatcaatcca agagttggac ctgctcccca gtacatacta
    17341 gaaaaacact accaacgtaa acatggacca aatgcttatt atggtcaaaa gtaagaacga
    17401 tgaaggagga gaaaaagata aatttagctc ctctttttac atttgatttt tattgagagt
    17461 aaagttggag ttgaagtaat tttgaaagat tttttag
//
LOCUS       DAVMEI010000026        16727 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVMEI010000026 DAVMEI010000000
VERSION     DAVMEI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.026"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            389..1567
                     /locus_tag="VYQ24_000697"
     CDS             389..1567
                     /locus_tag="VYQ24_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1967868.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTCINLVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            1881..2012
                     /locus_tag="VYQ24_000698"
     CDS             1881..2012
                     /locus_tag="VYQ24_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1967869.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2033..3583
                     /gene="dltA"
                     /locus_tag="VYQ24_000699"
     CDS             2033..3583
                     /gene="dltA"
                     /locus_tag="VYQ24_000699"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1967870.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPRLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3580..4824
                     /gene="dltB"
                     /locus_tag="VYQ24_000700"
     CDS             3580..4824
                     /gene="dltB"
                     /locus_tag="VYQ24_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1967871.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGIVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4838..5077
                     /gene="dltC"
                     /locus_tag="VYQ24_000701"
     CDS             4838..5077
                     /gene="dltC"
                     /locus_tag="VYQ24_000701"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1967872.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5070..6338
                     /gene="dltD"
                     /locus_tag="VYQ24_000702"
     CDS             5070..6338
                     /gene="dltD"
                     /locus_tag="VYQ24_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1967873.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6493..6933
                     /gene="adcR"
                     /locus_tag="VYQ24_000703"
     CDS             6493..6933
                     /gene="adcR"
                     /locus_tag="VYQ24_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1967874.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6933..7637
                     /locus_tag="VYQ24_000704"
     CDS             6933..7637
                     /locus_tag="VYQ24_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1967875.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7630..8436
                     /locus_tag="VYQ24_000705"
     CDS             7630..8436
                     /locus_tag="VYQ24_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1967876.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8446..9951
                     /gene="adcA"
                     /locus_tag="VYQ24_000706"
     CDS             8446..9951
                     /gene="adcA"
                     /locus_tag="VYQ24_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1967877.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11078..11851)
                     /locus_tag="VYQ24_000707"
     CDS             complement(11078..11851)
                     /locus_tag="VYQ24_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1967878.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            12027..13430
                     /locus_tag="VYQ24_000708"
     CDS             12027..13430
                     /locus_tag="VYQ24_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1967879.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSTLLPEIVSEGNVLGRIKKE
                     YGLSDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13545..14183
                     /locus_tag="VYQ24_000709"
     CDS             13545..14183
                     /locus_tag="VYQ24_000709"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1967880.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            14195..14635
                     /locus_tag="VYQ24_000710"
     CDS             14195..14635
                     /locus_tag="VYQ24_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1967881.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14628..15059
                     /locus_tag="VYQ24_000711"
     CDS             14628..15059
                     /locus_tag="VYQ24_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1967882.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15050..15520
                     /locus_tag="VYQ24_000712"
     CDS             15050..15520
                     /locus_tag="VYQ24_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1967883.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15545..16318
                     /locus_tag="VYQ24_000713"
     CDS             15545..16318
                     /locus_tag="VYQ24_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1967884.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16315..>16727
                     /locus_tag="VYQ24_000714"
     CDS             16315..>16727
                     /locus_tag="VYQ24_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1967885.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIF"
BASE COUNT         5048 a         2870 c         3540 g         5269 t
ORIGIN      
        1 actcccctga aatttatttt aattatgcaa atttcacgta tttttgatgc tgagacgacg
       61 atcctgggaa cttttcagat atttttttga ctatctaaat ctatcattag aaaagcttag
      121 agcgccaaag gatttgagcg tttttctgat ttttaagact ttttccagtc tctttttcga
      181 ttgaagatgt aattattcta ctaactaact aacttcttag tactagccaa caacgataat
      241 cataattcct cctaaaatta ggaataataa aggcaatagt ttttgttttt tcatgtaaaa
      301 aacctcactt ttgttttctg ctattttatg ctaaaatatt aaaaatcaaa tttaattcca
      361 aagtttgtaa ctaaaggggg agcgctacat gtctaattca tttgtcaagt tgttagtctc
      421 tcaattattt gcaaatttag cagatatttt ctttagagta acaatcattg ctaacatata
      481 cattatttca aaatcagtaa ttgccacatc actagttcct atcttaatag gaatatcctc
      541 ttttgttgcg agtcttttag ttccgttggt tactaaaagg ttagcgctaa atagggtttt
      601 atctttatct caatttggaa agactatatt attggcgata ctggtaggaa tgtttaccgt
      661 aatgcaatcc gtagcgcctt tggtgaccta tctatttgtt gttgcaattt ccatactaga
      721 tggttttgca gcacccgttt cctatgctat tgtgccacgc tatgcgaccg atttgggtaa
      781 ggctaattca gccttatcaa tgactggtga agctgttcaa ttgataggtt ggggattagg
      841 tggactcttg tttgcaacaa ttggtctgtt acctaccacg tgtatcaatt tagtcttgta
      901 tatcatttct agctttctga tgttatttct tcctaacgct gaagtggagg tgttagagtc
      961 agaaactaat cttgaaattt tgctcaaagg ttggaagtta gttgctagaa atcctagatt
     1021 aagacttttt gtatcagcaa atttattgga aattttttca aatacgattt gggtttcttc
     1081 cattatactt gtttttgtaa cggagttatt aaataaaacg gaaagttact ggggatattc
     1141 taatacagca tactctattg gtattataat tagtggctta attgctttta ggctatctga
     1201 aaagttcctt gctgctaaat gggaaagtat tctttttcct cttgtagcta tggcaatagt
     1261 gacactgact attctatatt ttccaaacgc acagatgttt ttattatttt cagctttagt
     1321 tggtatgtta tcgcaactaa aagaagttcc tgaaagtgta tttcttcagg aaacagtaga
     1381 ggaaaatcat ttagttaatg tctattccgt tcttgaagtg atttctacat tagcattttc
     1441 agtatttgtt ttgctaatga gctatattac tgagagtttt ggtattagca tcagtttttg
     1501 gctatcagcc atttgtctga tgatagaagc tattttaatt tatatcagac gagattattt
     1561 taaatgagag agtggttaaa aagtctttaa aaatcagaaa agcgcatagt atcaggtgtt
     1621 gaaaaaactt gatattgtgt gttttattat gggaagattt acttcattgt ctcctgaaat
     1681 ggagttttga agcaggatca ctctatttga aaatatacat agaaaaactg tataatcctt
     1741 aaaataagct gaaatctatt ggaaacatac ggtaaacaat tgaaaagata ctatataaag
     1801 tggtacaatg gtagaaaaat aagctagtaa gaatcactct tattttagcg aaaaattaca
     1861 gaaatgaatg gtttttcttg atgaaacaga gaaaagaatt gtacctcttt cttggtcgga
     1921 cagccttgta ttttcttatc tttctagggc tgctttactt ctttagctat cttggtcagg
     1981 gtcaaggaag ctttatctat aatgaatttt aacttgaagg agaatcccta ttgtgtcaaa
     2041 taaaccaata gcagatatga ttgaaaccat tgagcatttt gctcagacac agcctagcta
     2101 tcctgtctat aatgttttgg ggcaggaaca cacttatggc gatttaaagg ctgattcgga
     2161 tagtttggct gcagtcattg accaactagg cttgcctgag aagtctcctg tggttgtttt
     2221 tggtggccaa gaatatgaaa tgttggcaac ctttgtagcg ctgactaagt caggtcatgc
     2281 ctacattcca attgatagcc attcggcctt ggagcgagtt tcagctattt tagaagtagc
     2341 agagccaaga ttgattattg ccatttcagc ctttcccttg gagcaggttt ctacaccaat
     2401 gataaatcta gctcaggttc aagaagcctt tgcccaaggg aacaactatg aaatcacgca
     2461 tccagtcaag ggagatgata attactacat tatctttact tctggtacga ctggtaagcc
     2521 taagggagtg cagatttcac atgataatct cctcagcttt acaaactgga tgattacgga
     2581 taaggaattt gcgacaccga gtcgtccgca aatgctggca cagccacctt attcttttga
     2641 cttgtctgtc atgtattggg caccgacctt ggcacttggt ggtacgcttt tcactcttcc
     2701 ttcagtcatc actcaggact ttaagcaact ctttgcggct atcttttcat tgccaatcgc
     2761 tatctggaca tcaacaccat cctttgcaga tatggccatg ttgtctgaat acttcaacag
     2821 tgagaaaatg cctggaatca cgcatttcta ctttgatggt gaagaattga cggtcaaaac
     2881 agctcaaaaa ctgcgcgagc gtttcccaaa tgcccgtatc atcaatgctt acggcccaac
     2941 agaagcgaca gtagctctgt cagcagttgc cgtgacagac gagatgttag cgactctcaa
     3001 acgcctacca atcggctata ccaaggctga ttctccaacc tttatcattg acgaggaagg
     3061 aaataaactg ccaaatggtg agcagggaga aatcattgtt tctgggccag ctgtttcaaa
     3121 aggttatatg aacaatcctg aaaaaacagc agaagccttc tttgagtttg aaaatctgcc
     3181 agcctatcac acaggcgatg tgggaaccat gacagatgag ggcttgcttc tctacggcgg
     3241 acgcatggac ttccagatta agtttaacgg ttaccgcatt gagttagaag atgtctctca
     3301 aaacctcaac aagtctcgct ttatcgaatc tgctgtcgca gtaccgcgct ataacaagga
     3361 ccacaaggta caaaatctat tggcttatgt catcttaaaa gacggtgttc gtgagcagtt
     3421 tgagcgagat atcgatatta ccaaggccat caaggaagac ctgacagaca tcatgatgtc
     3481 ctacatgatg ccatctaaat tcctttaccg agacagtttg ccactgactc caaatggaaa
     3541 gattgacatc aaaggattga ttaacgaggt gaataagaga tgatggagtt ttttcaacag
     3601 cttcctcatt tagagccata tggcaatcct cagtattttg tttatgtgat tgctgcaacc
     3661 ttgcccatct ttataggtct ctttttcaag aaacgctttg cctggtatga agtgttggta
     3721 agtctcttct ttattgtcac catgttggtg ggtggaaaga ccaatcaact agctgccttg
     3781 ggtatttacc tttgctggga aatattgctc ctgcttttct acaagcatta tcgaaaaagc
     3841 aaggatggca agtgggtctt ctacttagtt agttttctgt ccctacttcc gattatcttt
     3901 gtcaaggtgc aaccagctat caatggaacg cagtctttgc ttgggttctt gggaatttct
     3961 taccttacct ttcgttcggt tggaattgtc atcgagctga gagatggagt gattaaggat
     4021 tttaccctct gggaattcct ccgtttcctt ctcttcatgc caactttctc gagtggtcca
     4081 atcgatcgct ttaagcgatt taatgaaaat tatcaggcta ttcctgagcg agatgagttg
     4141 atggatatgc tggatgaatc tgtccgctat atcatgtggg gctttttgta taagtttatc
     4201 ctagctcatg ttttaggaga gaccttacta cctcctctga agaatttagc cttgcagtca
     4261 ggtggcttct ttaatctcta tgccttggca gttatgtata cttttggtct ggagctcttc
     4321 tttgactttg caggttattc tatgtttgct ttggccatct caaacttgat gggaatccgt
     4381 agccctatca actttaacaa gcccttttta tcaagggatt taaaggagtt ttggaatcgc
     4441 tggcatatga gtctgtcctt ctggttccgt gactttgtct ttatgcgaat ggtgatggtg
     4501 ttaaccagaa agaaagtctt taaaaatcgt aatgtaacct caagcatggc ctacattgta
     4561 aatatgctga ttatgggatt ttggcatggc gtgacctggt actatatcgc ctatggactc
     4621 tttcatggac taggcttggt catcaatgat gcctgggttc gcaagaaaaa aacgctcaat
     4681 aaggaacgga aaaaagcagg gaaggctgcc ctacctgaga atcgctggat tcagttgctt
     4741 ggtatagttg tcactttcca tgttgtcatg ttgtcattct taatcttttc tggattcttg
     4801 aataatctat ggtttaaaaa ataaaaggaa ataaaatatg gatatcaaat cagaagttat
     4861 tgaaattatt gatgagttgt ttatggaaga tgtttctgac atgatggatg aagatctttt
     4921 tgatgcaggt gtcttggata gtatgggaac ggttgagttg attgtggaga ttgagaaccg
     4981 ttttgacatt cgtgtccctg taacagagtt tggtcgtgac gactggaata cagctaataa
     5041 aatcatagct ggtattgtgg agctacaaaa tgcttaaacg cttatggatg atcttcggac
     5101 cggtcttgat agctggtttg ttggtttttc tgctcatttt cttttatcct actgagatgc
     5161 atcataatct aggagctgaa aagcgttcag cagtggctac tactatcgat agttttaagg
     5221 agcgaagtca aaaagtcaga gcactatctg atccaaatgt gcgttttgtt cccttctttg
     5281 gctctagtga atggcttcgt tttgacggtg ctcatcctgc ggtattagct gagaaataca
     5341 atcgttccta ccgtccttat cttttaggac aggggggagc tgcatcgctt aaccaatatt
     5401 ttggaatgca acagatgtta ccacagctgg agaataaaca agttgtgtat gttatctcac
     5461 ctcagtggtt cagtaaaaat ggctatgatc cagcagcctt ccagcagtat tttaatggag
     5521 accagttgac tagttttctg aaacatcaat ctggggatca ggctagtcaa tatgcagcga
     5581 ctcgcttact gcaacagttc ccaaacgtag ctatgaagga cctggttcag aagttggcaa
     5641 gtaaagaaga attgtcgaca gcagacaatg aaatgattga attattggct cgttttaatg
     5701 aacgccaagc ctcctttttt ggtcagtttt cggttagagg ctatgttaac tacgataagc
     5761 atgtagctaa gtatttaaaa atcttgccag accagttttc ttatcaagca atagaagatg
     5821 ttgtcaaagc agatgctgaa aaaaatactt ccaataatga gatgggaatg gaaaattatt
     5881 tctataatga gcagatcaag aaggatttga agaaattaaa ggattctcag aaaagcttta
     5941 cctatctcaa gtcgccagag tataatgact tgcagttggt tttaacacag ttttctaaat
     6001 ctaaggtaaa cccgattttt atcattccac ctgttaataa aaaatggatg gactatgctg
     6061 gtctacgaga ggatatgtac caacaaacgg tgcagaagat tcgctaccag ttagaaagtc
     6121 aaggttttac caatatagca gatttttcta aggacggcgg ggagcctttc tttatgaagg
     6181 acaccattca ccttggttgg ttgggttggt tggcttttga caaggcagtt gatcctttcc
     6241 tatccaatcc cacaccagct ccgacttacc atctgaatga gcgctttttc agcaaagatt
     6301 gggcgactta tgatggagat gtcaaagaat ttcaatagat aatataatat agaagagttc
     6361 aagaatgaaa ctagttttca cgttttttct tgactctttt tttgcttgtg atataattaa
     6421 ctggtaaaca aaatgtcaaa gaatagtatt tttctcttaa aaaagagaag tctaaaagga
     6481 aaggagtcag atatgagaca gctagcaaag gatatcaatg cttttttgaa tgaggtgatt
     6541 ttgcaggcgg aaaatcagca tgaaatccta ataggtcatt gcactagcga ggtggccctg
     6601 accaatactc aggagcatat ccttatgctc ttgtcagagg aatctttaac aaattcagaa
     6661 ttggcccgtc gtctcaatgt cagtcaggcg gcagttacca aggccattaa gtctttggtc
     6721 aaggaaggga tgttggaaac atctaaagat tctaaagatg cgcgtgtgat tttttatcag
     6781 ttgactgact tggctcgtcc aatcgctgag gagcaccacc atcaccatga gcatacactt
     6841 ttaacctatg aacaagtggc gactcagttt actccaaatg aacaaaaagt gattcagcgg
     6901 tttttgactg ctttagtagg agaaatcaaa taatgagata tattacggta gaggatttgt
     6961 ccttctatta tgataaggag cctgttcttg aacatatcaa ttatagtgtt gatagtgggg
     7021 aatttgttac cttgactggg gaaaatggag cggctaagac gacgctcatc aaggccagtc
     7081 ttggaattct gcaaccacgc attggaaagg tggctatttc aaagacaaat acgcaaggta
     7141 agaaattgag aatagcctat cttcctcaac aaattgccag ttttaatgct ggttttccaa
     7201 gtacggtcta tgaatttgtc aagtcgggtc gctatccgag aaaaggctgg ttccgtcgtt
     7261 tgaatgctca tgatgaggag catatcaagg ctagtctgga ctcagttggc atgtgggaac
     7321 atcgagacaa acgcttgggg tctctatctg ggggacaaaa gcagcgagcg gtgattgcgc
     7381 gtatgtttgc ttctgaccct gatgtgttta tcctagacga gccgacaacg gggatggatg
     7441 caggaagtaa aaatgaattt tacgaactca tgcaccacag cgcccatcat catggcaagg
     7501 ctgttttgat gattacccat gaccctgaag aagttaagga ttatgcggat cgcaatattc
     7561 atctagtccg taaccaagac tcgccatggc gttgtttcaa tgttcatgag aatggccagg
     7621 aggtgggcca tgcttagttt attatcttac gactttatac aacgcgcctt tttggcggtt
     7681 attgctatga gtcttttctc accggtattg ggaaccttcc tcatcttgcg tcgtcagagt
     7741 ttgatgagtg atacccttag ccacgtctca ctttcaggtg tagcctttgg tctggttttg
     7801 gggatttctc caactgtttc tactattgcc attgtcttga ttgcggcggt ctttctggag
     7861 tatctccgta cggtttacaa gagctttatg gaaatcggga cagctatcct catgtcaaca
     7921 ggtctggctg tttctctgat tgtcatgagc aagggtaaaa gctcgagttc aatgagtttg
     7981 gaccaatatc tctttggttc gatcgtgact atcagtgaag aacaggtcat ttccctcttt
     8041 gtcattgcgg cggttgtttt gattttgacc tttctctttc ttcgtcctat gtatatctta
     8101 acttttgacg aagatacggc ctttgtggat ggcttgccag ttcgtaccat gtccattctt
     8161 tttaacatgg tgacaggggt ggctattgcc cttatgattc ctgcagcagg agctcttctg
     8221 gtatcgacca ttatggtctt gccagctagt attgccctgc gtctggggaa aaactttaaa
     8281 tcggttatgc tgcttgccag tgcgattggc tttttgggaa tggtagcagg actttacatt
     8341 tcctactatg cagaaacacc tgcaagtgca agtattacca ttatttttgt aactgtcttt
     8401 atactaatca gtttagtaag acgttttatc aaataggaga cgaacgtgaa aaaaattagc
     8461 ttattactag ccagtctatg tgccttgttt ttagtggctt gttccaatca aaaacaggca
     8521 gatggtaaac tcaatatcgt gacaaccttt taccctgtct atgaatttac caagcaagtc
     8581 gcaggagata cggctaatgt agaactccta atcggtgctg ggacagaacc tcatgaatac
     8641 gaaccatctg ccaaggcagt tgccaaaatc caagatgcag ataccttcgt ttatgaaaat
     8701 gaaaacatgg aaacatgggt acctaaattg ctagatacct tggataagaa aaaagtgaaa
     8761 accatcaagg cgacaggcga tatgttgctc ttgccaggtg gcgaggaaga agagggagac
     8821 catgaccatg gagaagaagg tcatcaccat gagtttgacc cccatgtttg gttatcacca
     8881 gttcgtgcca ttaaactagt agagcacatc cgcgacagct tgtcagcaga ttatcctgat
     8941 aaaaaagaga cctttgagaa gaatgcagct gcctatatcg aaaaattgca agccttggat
     9001 aaggcttacg cagaaggctt gtctcaagca aaacaaaaga gctttgtgac tcaacacgca
     9061 gcctttaact atcttgcctt ggactatgga ctcaaacaag tcgcaatctc aggactttct
     9121 ccagatgcag agccatcagc tgctcgcttg gcagaattga cagagtatgt caagaaaaat
     9181 aaaatcgcct atatctattt tgaagaaaat gcctcacaag cccttgctaa cacactttca
     9241 aaagaagcag gtgtcaaaac tgatgtcctc aatcctttag aaagtctgac agaagaggac
     9301 accaaggctg gagaaaatta catctctatc atggagaaaa acctcaaggc tttgaaacaa
     9361 acaacagacc aagaaggccc agcgatcgag cctgaaaagg cagaggatac caagacagtc
     9421 caaaatggtt acttcgagga tgcagctgtc aaggaccgca ccttgagtga ctatgcaggt
     9481 aactggcaat cagtttatcc tttccttgaa gacggcacgt ttgaccaagt ctttgactac
     9541 aaggctaagt tgactggtaa gatgacccag gctgagtaca aggcttacta tacaaaaggc
     9601 tatcagacag atgtgactaa gattaacatt actgataata ctatggaatt tgttcaaggt
     9661 ggacaaagca agaaatatac ttacaagtat gtcggtaaga aaattttgac ttacaagaaa
     9721 ggcaatcgtg gcgtgcgttt cctctttgaa gccacagatg ctgacgctgg acaattcaag
     9781 tatgttcagt ttagtgacca caatatcgcc ccagttaagg cagaacattt ccatatcttc
     9841 tttggaggca caagccaaga agccctcttt gaagaaatgg acaactggcc aacctactac
     9901 ccagataacc tatctggcca agaaatcgct caagaaatgt tggcgcattg atgagaaatc
     9961 gactagttca taagagctag tcggtttttt gatactagat ggctaacttc attatagacc
    10021 tttcagaact aagggaacct gaaaaaataa ataaaactct tgaaatatct gggcttatat
    10081 gctagaatgt agttagtgta taaggttcat tagaacacca aagcccctaa ctagtgccat
    10141 agttaagggc ttttttgacg tatcttagcg atttaacggg tgtttatagg ctagtcagtc
    10201 ggtctacttc ttaccatcta gccatttgca gacgaaatac aagataattc cagctataac
    10261 atctgctata atagtaagag caagctctgg tataaggctc attagtttca cctcctctca
    10321 cgaaccgtaa ggaacgtaat cggtaaccga tgacaaaaat agtatatcac attaaattta
    10381 gatcatcaac attgcttaat gcttgaaata ttaggactta tatgctaaaa tgtagttaat
    10441 tgataaggtt cattagaaca ccaaagcccc taactatggc cgtagttaag ggcttttttg
    10501 acgtatctta gcgatttaac gggtgtttat aggctagtca gtcggtctac tccttactat
    10561 ctagccatct gcagacgaaa tacaagataa ttccagctat aacatccgct ataatagtaa
    10621 gagcgagctc tatgataagg ctcattagtt tcacctcctc tcacgaaccc ataggaacgt
    10681 aatcggtaac cgatgacaaa aatagtatac cacaatacat ttagatcatc aaggtcactt
    10741 aattcttgaa atatagggtc taggcgacaa agtctttaaa atcagaaaaa cgcataatat
    10801 caggtgtgca aaaacttgat actatgcgtt ttattgtggg aatatttgcc ccatttctcc
    10861 tgaaattgag tttttgtcca gcctctgttt ttagggttgc taagaaaata atgtcatgtg
    10921 gtgaatattt gtaaatcagt cagcagacag aacgatactc ttcgaaaatc tcttcacatc
    10981 atgtcagctt cgtcttgccg tgtccatggg ggccttgact tcatcagttt tcaaatctgt
    11041 actgtgagca agctgagact agcttcctat ttgattttta ttgaatatca gaaacccatt
    11101 ctccatcaaa taattcgact gcgtctaata atttttggtc tggcaaggtg tcagaaataa
    11161 aggttgtgta tttggatagg ggattaattt taaaaaatcc agtcttgtaa aatttagaac
    11221 tatcaatcag taagatggtt tcatgggctt tgtcaataat attctttttt gaaatagctt
    11281 ggctgagaga agcttcataa acatattggt catcaatacc tcttgctgaa caaaatgcta
    11341 aatcgatatt aaaatgatct aataaagaat tttccttatc atagttgacc acggaacagg
    11401 attgatgttt gacctcgcca gatgtgataa agattttgga gctatcttta acagtttcag
    11461 atagggtttg tgcagtatgt aaaccatttg taaaaataat caaattatca agttcagaaa
    11521 gatagggaca aagttcgtag acagtagtac tagaatctag atagatacac ataccagacc
    11581 gaataaagtc tttagcgaga ctagcgatta gtcttttttg cctagtactt tctccttccc
    11641 gtatttgatg agaaagttca attgtgttca tagaggacag ggtcacgtat ccgtgctttc
    11701 ttttgataag accttgattt tctaagaaaa ttaaatcacg acgtaaggta cttgtgctgg
    11761 agaaagtgat ttctgccagc tcttttacgg caattctttt tttctttttg atgatttcaa
    11821 tcaattcaag tacacgttca tcttttatca taagctcctc ctaatttatc atttcaacta
    11881 tattatagca caaattggag gaatgtgaat tatttttatg aatattgggt taacatttga
    11941 acattattca agtaagcgtt cacatattga aaaaataaaa cgtggggatt ataataaagt
    12001 taatcaagga cgaagagaga agaaaaatgg aagcggtttt agcaatagat ttaggtgcga
    12061 cttctggaag agcaatcgtt ggttaccttt ctgaaaataa actagtaatg gaagaaataa
    12121 atcgcttttc taatctacct attagagtaa aagggcattt atcttgggat gttgactttc
    12181 tactagctaa aattcttgaa agtatccgct tggctaatac tagttacaag attttatcta
    12241 tcggtattga cacatgggga gttgattttg gactgattga taatgaaggt aagctgttat
    12301 cacaacctgt tcattatcgt gatgaaagaa caaagggagt gttaaaggaa atatctgaaa
    12361 tgactgaatt agaaaaactg tattcagaga caggaaatca gattatggag ataaatacct
    12421 tgtttcaact ctttaaggca cgtcaagaat ctcctgactc tttctataag accaataaga
    12481 ttcttttaat gccagatttg tttaattatc tcttgacagg taagtttgct acagaaaaaa
    12541 gcattgcttc aacaactcaa ttatttgatc ctaggagtca aaattggaat cagaatatct
    12601 taaaactatt tgaattggat tcaactttac ttcctgaaat tgtttcagag ggaaatgtcc
    12661 ttggaaggat aaaaaaggag tatggtttaa gcgatattcc tgttgtgaat gtttgtagtc
    12721 atgatacagc aagtgcgatt gtctcagtac ctaagacaga aggtagttta tttatttcat
    12781 caggtacttg gtctttggtt ggagtggaac ttacttcacc gattcttact accgaatcct
    12841 tcagttatgg atttacaaat gaagtcggta aagatggagt gattacattt ctgaagaatt
    12901 gtacagggtt gtggatcata gaggaactaa gacgttcatt tgaacgaaga gggaaagcct
    12961 attcttttga tgatattagg acaatggtgg agaaagaaaa agaaaatctt cctctgattg
    13021 atactgaatc aactgaattt gcaacagaat ctgatatgca caagactttg acagaatatc
    13081 tagcttatca tcatgaaact agagagtgga cagatggaca actatttaag attgtttatg
    13141 aaagcctagc tgaaacgtat aggaaagcga tagagttact agaagaacta actcataagg
    13201 tttataagag gatatatgtg attggaggag gtgctagagc cagttacttt aaccaaatga
    13261 ttgctgatag aactggtaaa gaggttctta caggtttgac tgaggctaca gctgtgggga
    13321 atattgttgt gcagctcata gctatgggac aattaaaagg gatggaagag gctcaccatg
    13381 ttattgagga gtttctacaa ttagagagtt attactccca aaagaattaa aaagattgag
    13441 agtttgtaaa tttgcctccc tcccccttct tagcttttgt gcaggaaggg gggataattg
    13501 gtgaattgaa aaatatttag tgttttgata tgaggaggac aaggatgtca gatgtaaaac
    13561 aagaattaat taaatatggt aagaagctag tagaaacaga tttgacgaaa ggaacaggtg
    13621 ggaatctcag cgttttcgat cgtgaaaaac aattgatggc aattaccccg tcgggtattg
    13681 atttctttga aatcaaagaa tccgatattg tagtgatgga tattaatgga aatgttgtag
    13741 agggagaacg cttgccatct agcgaatggt atatgcattt gattcaatat caaactcgtg
    13801 atgatatcga tgcaattatc catgctcata caacttatgc aacagtatta gcttgtctca
    13861 gagaaccact tccagcgagt cattatatga ttgcagtggc agggaaagat gttcgggtag
    13921 ctgagtatgc aacatatggc acgaaagaat tggctgtgaa tgcagctaaa gcaatggaag
    13981 gtcgtagagc agttttacta gcgaatcatg gaattttagc aggtgcacaa aatttattga
    14041 atgcatttaa tattgttgaa gaagttgaat attgtgcaaa aatttattgt ttagctaaga
    14101 attttggaga gccagtagtt cttcctgatg aggagatgga attgatggca gaaaaattta
    14161 aaacatacgg tcagagaaaa tagggaggat attaatgtta aaacatatac cgaaaaatat
    14221 ttctccagat ttattgaaga ctttaatgga aatgggacat ggagatgaaa tagtattagc
    14281 tgacgcgaat tatccttctg cctcatgtgc aaataagcta attcgttgtg atggcgtaaa
    14341 tattccagaa ttattagatt ccattctgta tttaatgcca ttagatagtt acgtcgatag
    14401 ttcaattcag tttatgaacg ttgtttcggg tgatgatatt cctaagatat ggggtaccta
    14461 tagacagatg attgaaggtc atggtacaga tcttaaaacg attacttatc ttagaagaga
    14521 agacttttat gaacgtagta agaaagctta tgctattgtt gctacaggag aaacttcact
    14581 ttatgctaat attatcctta agaaaggagt agttgttgaa agagaaaatg tttaatagag
    14641 gaattttagt tgccagtcat ggtgattttg ctagcggagc tctcatgacc gcagaaatgt
    14701 ttgttggtga gacaacaaat gatagagtta ggacattagg tttgatgcct ggagagaata
    14761 ttgtagagtt tgagcattat tttaaaaatc aagtggatga actgttagac tcaaatcaag
    14821 aggttatcgt tttgactgac ttgattggag gaagtcctaa taatgtggct ttgtcacggt
    14881 ttttaaattt ggattcagtt gatattgtaa cagggtttaa tattcctctc ctagtggaat
    14941 taatatcaaa ttatgattca aaaatcaatt tagaagaaat tgttcacaat gctcaaaata
    15001 gtttgtttaa tgttaaacaa caacttaacg tagaggagga agaagattta tgtctataga
    15061 gtttgttcgt attgatgacc gtctggtaca tggtcaagtt gtcactacgt ggctaaaaaa
    15121 gtatgatatt gagcaagtta tcattgttaa tgatcgcatc tcagaagata aaacacgaca
    15181 atctatttta aagatttctg caccagtagg tttaaaaatt gttttcttta gtgtaaaacg
    15241 gtttgtggaa gttttaaact ctgtgccaat aaaaaagaga acaatgctga tatatacaaa
    15301 tccaaaagat gtgtatgatt ctattgaagg aaatttaaaa ttggagtacc tcaatgtagg
    15361 acagatgagt aaaacggagg aaaatgaaaa ggtaacggga ggtgtagctc taggtgaaga
    15421 agacaaatat tattttaaga aaatagttga taagggaacg agagttgaaa ttcaaatggt
    15481 tcctaatgat aaagttacaa tgttggaaaa atttttataa aaatagttta aggaggtaca
    15541 gtatatgcta ttcacacaag cattactggt gacattagtt gggattattg ccactattga
    15601 ctataatgga ccgttattta tgatccaccg tccgttagtt acaagtgcaa tggttggctt
    15661 agtcttagga gatttcaccc aaggtgttct tattggttca gctcttgaat taacttggct
    15721 cggtgtaaca ggtattggag gttacactcc accagatact atttcaggtg cgattattgg
    15781 tactgcattt ggtattttat ctggtcaagg agaaactgct ggtatcgcta tagcagttcc
    15841 aattgcagtt gctacccaac agttggatgt tcttgcaaaa actttagatg tttattttgt
    15901 gaaaaaagct gataatgatg ctaaaaacgg agattattca aagatcggtt tttatcatta
    15961 ttcaagtttg gttttaatca cgttatttaa aattgtacca attttcctag ctattatgct
    16021 tggaggggaa tatgtggcag acttgtttgc taaggttcca ccaatcgtta tgcagggact
    16081 taactctgca ggtgctttac taccttcaat tggttttggt atgcttttaa atatgatgct
    16141 caagaaaaat atgtgggtat tcttgttgat tggattcatt tgttctgtgt atggaggaat
    16201 gtcaaccatt gggatctcac tagttggtat tgcggtagca tacttctacg atatgattgg
    16261 aagtaaacca caagaaacaa cttcaagtag tgatgttgag gaggatcttg atctatgatg
    16321 aataataaag taactaaagt tgaacttaaa aaagttttca aacgaagttt tatgtatggt
    16381 tcttcatgga actatgagag aatgcagaac ctaggttttc tatatacaat tcttccagta
    16441 ttgaaaaaac tatacccaga caaagattca gcttctcctg caatgaaacg tcaccttgag
    16501 tttttcaata ctcatcaaac agcggcacca tttattcttg gagttacttc cgctatggaa
    16561 gaacaagaag gaaatgaagg tgcagcttca attactggta ttaaagttgg cttgatgggg
    16621 ccactggctg gtctaggaga tagtttgttc tggctgacac tagttcctat ctgttttagt
    16681 attggtgcgt cttattctaa agacggcggt gctttaggta tctttat
//
LOCUS       DAVMEI010000027        16419 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVMEI010000027 DAVMEI010000000
VERSION     DAVMEI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.027"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(117..833)
                     /locus_tag="VYQ24_000715"
     CDS             complement(117..833)
                     /locus_tag="VYQ24_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1967886.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1181..2968
                     /locus_tag="VYQ24_000716"
     CDS             1181..2968
                     /locus_tag="VYQ24_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1967887.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2965..3441
                     /locus_tag="VYQ24_000717"
     CDS             2965..3441
                     /locus_tag="VYQ24_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1967888.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3469..4374
                     /locus_tag="VYQ24_000718"
     CDS             3469..4374
                     /locus_tag="VYQ24_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1967889.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4361..5176
                     /locus_tag="VYQ24_000719"
     CDS             4361..5176
                     /locus_tag="VYQ24_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1967890.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            5183..5587
                     /locus_tag="VYQ24_000720"
     CDS             5183..5587
                     /locus_tag="VYQ24_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1967891.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            5886..7052
                     /locus_tag="VYQ24_000721"
     CDS             5886..7052
                     /locus_tag="VYQ24_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1967892.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            7168..8205
                     /locus_tag="VYQ24_000722"
     CDS             7168..8205
                     /locus_tag="VYQ24_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1967893.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            8412..9266
                     /locus_tag="VYQ24_000723"
     CDS             8412..9266
                     /locus_tag="VYQ24_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1967894.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGIGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     RGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSKLEITDSDGI
                     VTRIRLAR"
     gene            9416..9652
                     /locus_tag="VYQ24_000724"
     CDS             9416..9652
                     /locus_tag="VYQ24_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV1967895.1"
                     /translation="MTNSKYITRLKRSEGQLRGIQKMIEGDRDCADIVTQLTAVKSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAI"
     gene            complement(10048..10140)
                     /locus_tag="VYQ24_000725"
                     /pseudo
     CDS             complement(10048..10140)
                     /locus_tag="VYQ24_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10176..10472)
                     /locus_tag="VYQ24_000726"
     CDS             complement(10176..10472)
                     /locus_tag="VYQ24_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1967896.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNTWPGQVE"
     gene            complement(10462..10623)
                     /locus_tag="VYQ24_000727"
     CDS             complement(10462..10623)
                     /locus_tag="VYQ24_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1967897.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            10693..11457
                     /locus_tag="VYQ24_000728"
     CDS             10693..11457
                     /locus_tag="VYQ24_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1967898.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            12070..13449
                     /locus_tag="VYQ24_000729"
     CDS             12070..13449
                     /locus_tag="VYQ24_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1967899.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            13463..14128
                     /locus_tag="VYQ24_000730"
     CDS             13463..14128
                     /locus_tag="VYQ24_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1967900.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            14438..14976
                     /locus_tag="VYQ24_000731"
                     /pseudo
     CDS             14438..14976
                     /locus_tag="VYQ24_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            15076..15453
                     /locus_tag="VYQ24_000732"
     CDS             15076..15453
                     /locus_tag="VYQ24_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1967901.1"
                     /translation="MIRREDQSVNEQVMIKCIDQQLKVNRLLVDQLDLSQVSHPKMRE
                     YLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAIRKTMLSRLTKH
                     SVLPDRKLSNVVYQITKSVYGFN"
     gene            15569..>16419
                     /gene="pavB"
                     /locus_tag="VYQ24_000733"
     CDS             15569..>16419
                     /gene="pavB"
                     /locus_tag="VYQ24_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1967902.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYSATVNVYGNKDGK
                     PDLDNIVA"
BASE COUNT         4588 a         3090 c         3721 g         5020 t
ORIGIN      
        1 tttctataaa atattccaca aattatagaa tcttccaaaa ataggtaagc gctacctttt
       61 tggtgtggta taataagcat agaaaaagcc cgagcgatta ggctcaggct ttcttcttag
      121 tgatcacggt cacatgagat gaatttaatc ttgtagtaat cagatcgttt gtaagtttca
      181 ctgtattcta aaacttggcc agttgattcg agtttggtga ttttagtttg taggacagta
      241 gggaattgtt catcgactcc gaggactgaa gccgcatgtt ctggagttgg aaagactatt
      301 tcgttgattt cttcaaagtg ttcatcattc atgtgaatgt ggtagtctaa cttgaaacga
      361 ttatagatag aactatagta ttcaaggttt ggataatttg cgttgatata ttgttctggg
      421 atgtaggatg tatggtagat ataaacgaca ccgtttgatt cgcggatacg ttcaatcttg
      481 tagtagaatt gatcgccgcg tagacccaat ttttccaagt aaacaagctt gtttccgcgt
      541 tcaattgaaa gaacagttac cttatcatct ttagcattga agagttcaat atctgaaaac
      601 tctacaagct tgtgtttgcg tgcacgtgaa acgaaggttc cttttccttg ttggcggaca
      661 atatagccat ctttggcaag gtcgtttaag gcgcgaacaa ctgtgataga gctgacatcg
      721 tacattgaaa tgagttctgc ttcagtgtaa aatttatctc cactgctaaa ctgcccagag
      781 atgattttat tttttaattc gtcttttatg tattgatatt tgggaattgc catattttta
      841 cctctatttt ccttctccat agaaaatttt accataaaag cgaaaaaaat agtagtcttt
      901 tgaataaaaa attagaataa tagtttgaaa aattaagcga tatagggatt gctcatattt
      961 tatcaatgta ttctatacaa aattatttta ggtcctatat tccgaattta aagagcgttt
     1021 gaaatgggtc taaaaatgtt tgaaactaat tgaatgtgtg tataatagta aaaatgcagt
     1081 aaatatcccg acaatttcta ttgacaattc aaacagattg gtttataatt aatataacaa
     1141 caaatgaaat cgcaaacttt cgcggtcgga aggtagtttt atgacacgat ttgagatacg
     1201 agatgatttc tatctcgatg gaaaatcatt taagatttta tctggtgcca ttcattattt
     1261 tagggttcct ccagaggatt ggtatcattc gctctataac ttgaaggctc ttggttttaa
     1321 tacggtagag acttatgttg cttggaattt acacgagcct cgtgaaggtg agtttcattt
     1381 tgaaggtgat ctggatttag agaaatttct ccaaatagcg caggatttgg gtctctacgc
     1441 aattgtgcgt ccgtctccat ttatctgtgc ggaatgggaa ttcggtggct taccagcttg
     1501 gctcttgacc aagaacatgc gaattcgctc atccgaccca gcatatatcg aggcagttgg
     1561 tcgctactat gatcagttat tgccaagact ggtgcctcgt ttgttggaca atggtggcaa
     1621 tattctcatg atgcaggttg aaaatgagta tggttcttac ggagaagata aggcttacct
     1681 gagagcgatt cgacagctaa tggaagagtg tggcgtaacc tgtcccctct ttacatcaga
     1741 tggtccatgg cgagctactc tgaaagctgg aaccttaatt gaagaggacc tctttgtaac
     1801 aggaaacttt ggttctaagg caccttacaa cttttcgcag atgcaggaat tctttgatga
     1861 acatggtaag aaatggccac tcatgtgtat ggagttctgg gatggttggt tcaatcgctg
     1921 gaaagaaccg attatcacac gggatcctaa ggaattggca gatgcagttc gagaggtttt
     1981 ggaacaaggc tctatcaatc tttacatgtt ccacggtggt acaaactttg gtttcatgaa
     2041 tggttgctca gctcgaggaa ctttggacct gccacaagtt acgtcttatg attacgatgc
     2101 ccttctggat gaagaaggaa atccaactgc taaatatctt gcagtcaaga agatgatggc
     2161 aacacatttt tcagagtatc cgcagttgga accactctac aaagagagta tggagttgga
     2221 tgctattcca ctagttgaaa aagtttcttt gtttgaaacc ttagatagct tgtcaagtcc
     2281 tgtagaaagt ctctatcctc aaaagatgga ggagctggga caaagttatg gctacctact
     2341 ttatcgaaca gaaacaaact gggatgcaga agaagaaaga cttcgtatca ttgatggtcg
     2401 agatagggcc cagctgtatg tcgatggtca gtgggttaaa actcaatatc agacagagat
     2461 tggggaagat attttttatc aaggtaaaaa gaaagggcta tctaggttag atatcttgat
     2521 agaaaatatg gggcgtgtca actatgggca taagttctta gcggatacgc aacgtaaggg
     2581 aattcggaca ggggtctgta aggatctgca tttcttacta aactggaaac actatccact
     2641 cccactagac aatcctgaga aaattgattt ttcaaaagga tggactcaag gacaaccagc
     2701 cttttacgct tatgacttta cagtcgaaga gccaaaagat acttacctag acttgtctga
     2761 gtttggtaag ggggttgcct ttgtcaatgg gcagaatcta ggacgttttt ggaacgttgg
     2821 cccaactctc tcactttata tccctcatag ctatctcaag gaaggtgcca accgcatcat
     2881 tatctttgaa acagaaggtc aatataaaga agagattcat ttaactcgta aacctacact
     2941 aaaacatata aagggggaaa acttatgaca attgtaggat gccgtattga tggacgtttg
     3001 atccacggac aagtagccaa tctttgggct ggaaaactaa atgtttcacg cattatggtt
     3061 gtagacgacg aagttgtcaa caacgatatt gaaaagagtg gtttgaaact tgcgacacca
     3121 ccaggtgtga aattgagtat tttgccagtt gagaaagctg cagccaatat tcttgctggc
     3181 aaatacgata gccaacgtct ctttatcgtg gctcgtaaac cagaccgctt ccttggtttg
     3241 gtagaagcag gtgtaccact tgaaaccctt aatgttggga atatgtctca aacaccagaa
     3301 actcgttcta ttacacgttc tatcaacgta gtagacaagg atgtggaaga cttccacaaa
     3361 ctggcagaaa aaggtgttaa acttactgct cagatggttc caaatgatcc aatttcagac
     3421 tttttgagct tattaaaata ggaaaaaatt ttttaggagg tcattgttat gatacaatgg
     3481 tggcaaattt tacttctcac tttgtactca gcttatcaaa tctgtgatga gttgacgatc
     3541 gtttcatctg caggttcccc tgtatttgct ggtttcatta ctggtttaat catgggagat
     3601 gtgactactg gtttacttat cggtggtaac ttgcaactgt tcgttcttgg ggttggtacc
     3661 ttcggtggtg cttctcgtat cgacgcaact tctggtgcgg ttcttgcgac agccttctct
     3721 gtttcacaag gaattgatgc accgcttgca attactacaa tcgctgtacc agtagcagct
     3781 ctcttgactt acttcgacgt tcttggtcgt atgactacta ccttcttcgc tcaccgtgtg
     3841 gatgctgcaa tcgaacgctt tgactataaa ggtattgaac gcaactactt gcttggtgcg
     3901 attccgtggg ctctatctcg tgcccttcca gtcttctttg cccttgcttt tggtggtgcc
     3961 tttgtacaat cagtagtaga cttcgttgaa gcctacaaat gggttgcaga tggcttgaca
     4021 ctcgcaggac gtatgcttcc aggtcttgga tttgcaatct tgcttcgtta ccttccagtt
     4081 aaacgtaacc ttcactacct tgctatggga tttggtttga cagctatgtt gactgttctt
     4141 tactcatatg taacaggtct tggtggcgct gttgctggta tcgtaggtac tcttcctgct
     4201 gaagttgctg aaaaaattgg tttcgtgaac aacttcaaag gtttgtctat gattggtatt
     4261 tctatcgtag gtattttcct tgcagtgctt cacttcaaaa atagccaaaa agtagctgta
     4321 gcagcacctt ctacaccatc agaaagtggg gaaatcgaag atgacgaatt ctaattacaa
     4381 acttacaaaa gaagatttta atcaaatcaa caaacgtagc ttgtttactt tccaattagg
     4441 ttggaactac gaacgtatgc aagcttctgg ttacctttac atgatcttgc ctcagttgcg
     4501 taaaatgtat ggtgatggaa ctcctgaatt gaaagaaatg atgaaagttc atactcaatt
     4561 cttcaatact tcaccattct tccataccat tatcgctggt tttgaccttg ccatggaaga
     4621 aaaagatggt gtaggttcaa aagacgctgt taacggtatc aagacaggtt tgatgggacc
     4681 attcgctcct cttggggata caatctttgg ttcacttgta cctgctatca tggggtcagt
     4741 cgcagcaact atggctatcg ctggccaacc ttgggggatc ttcctttgga ttgcagttgc
     4801 agtagcgtat gacatcttcc gttggaaaca gttggaattt gcttacaaag aaggggttaa
     4861 ccttatcaac aacatgcaaa gtaccttgac agctttgatt gacgctgcat ctgtacttgg
     4921 tgtcttcatg atgggtgctc ttgtagcaac agtgattaac tttgaaattt cttacaagtt
     4981 gccaatcggt gaaaagatga ttgatttcca agacatcttg aaccaaatct tcccacgttt
     5041 gcttccagca atctttactg cctttatctt ctggttgctt ggtaaaaaag gtatgaactc
     5101 tactaaagct atcggtatta ttatcgtact tgctttggct ctttctgccc ttggtcactt
     5161 tgcacttgga atgtaattcc ttatgactaa atcattaatt ttggtgagcc atggtcgctt
     5221 ctgtgaggag cttagaggta gcacagaaat gattatgggc ccacaagaca acatttacac
     5281 agtagctctt cttccagaag atggcccaga agaatttact gctaaatttg aagctgttat
     5341 tgaaggattg gatgatttcc tagtctttgc ggatcttctc ggtgggacac cttgtaatgt
     5401 ggtgagtcgc ttgatcatgg aaggtcgtga tattgacctt tacgcaggga tgaatcttcc
     5461 aatggtgatt gaatttatca atgcgagcct tacaggcgca gatgcggact acaagagccg
     5521 tgctgcagaa agcattgtga aagttaatga cctgttagcg ggcttcgatg atgacgaaga
     5581 tgaataatac tcttcgaaaa tctcttcaaa ctacgtcaac gtcgccttgc cgtaggtata
     5641 tgttactgac ttcgtcagtc ttatccggca acctcaaaac ggtgttttga gctgacttcg
     5701 tcagtcttat ccggcaacct caaagcagtg ctttgagcag cctgcggcta gtttcctaca
     5761 gattttagtt ggaactcgat tcaattcatg tgacaacgtg aaaatcgtta gagcatttta
     5821 tatagaatat acatgggaat ggagcttact cccattccca tatttaatag aaaaagagga
     5881 actcaatgct acattataca aaagaagact tgctcgaatt gggtgcagaa atcactacgc
     5941 gtgaaatcta ccaacagcct gatgtatgga gagaagcttt tgaattttat caagcaaaac
     6001 gtgaagaaat tgcagccttt ctacaagaaa tcgctgataa acatgactat attaaggtta
     6061 tcttgacagg tgctgggact tctgcttatg tgggagatac cttgctacct tactttaagg
     6121 aagtctatga cgaacgcaaa tggaatttca atgctattgc gacaacagat atcgttgcca
     6181 aaccagcaac ctatttgaaa aaagatgtgg caactgtcct tgtgtctttt gctcgtagtg
     6241 ggaactcgcc tgaaagtgtg gcgactgttg atttggccaa atccttggtg gatgagcttt
     6301 atcaagtgac gattacttgt gcagcagatg gtaaattggc tcttcaagct cacggtgatg
     6361 accgtaatct cttgctcttg caaccagctg tctctaatga tgctggattt gccatgactt
     6421 ctagctttac gtctatgatg ttgacagctc tcttggtctt tgatcctaca gaatttgctg
     6481 ttaagtctga acgttttgaa gttgtatcta gtcttgcccg taaagtttta gacaaggtag
     6541 aagatgtcaa agagctcgtt gatttagact ttaaccgtgt catctatcta ggcgctggtc
     6601 ctttctttgg acttgctcat gaagctcagc tcaagatttt ggaattaact gctggtcaag
     6661 ttgcgaccat gtatgaaagc ccagttggct tccgtcacgg tccaaaatct cttatcaacg
     6721 acaatacagt tgttttggtc tttggtacaa cgacagccta cactcgtaag tacgacttgg
     6781 acttggttcg tgaagttgct ggtgaccaga ttgctcgtcg tgttgtgctt ttgagtgatc
     6841 aagcttttgg tcttgaaaat gtcaaagaag tggtccttgg ttgtggcggt gtcttgaatg
     6901 atatttaccg tgtcttccct tacatcgttt atgcccaact ctttgctcta ttgacttcac
     6961 tcaaggtaga aaataaacca gatacaccgt ctcctacagg tacagtaaac cgtgtagtac
     7021 aaggtgtcat aattcacgaa tatcaaaagt aagacagtgt ttatgaattc ttgacaagag
     7081 gatttgtaaa ttatcagata aaccatagat tgtcagtacg ctttctatgg tttgtttgct
     7141 tgagagaaat agtaaaagga gaacagaatg aaagcataca cagagcgtgt atttggaaat
     7201 gttgagggcg aggatgtctt ggcctatcga tttgagacag acggtggcta ccaacttgag
     7261 gttatgactt atggcgcgac catcttgcgc tatgtcgcac ctgacaaggc tggaaatttt
     7321 gccaatgtta tcttgggatt tgatgacttt gatagttatg taggcaatag tcccaagcat
     7381 ggagcaagtg taggtcctgt agcgggtcgt attgcaggtg cgacctttga gctcaatggt
     7441 aagacctatg accttgaggt taataatgct agcaactgta atcacagtgg ttcaactggt
     7501 tgggattcca gcctgtttga agttgaagaa gtaagcgatc atggcttgac tctctacaca
     7561 gagcgtacag atgggacagg agggttccct ggaaatctca agatttggat cagttatcac
     7621 ttggaagaaa ctggtgccta tgaaatcatc tacaaggtaa cgaccgatca ggatacgctg
     7681 gtcaatccaa ccaaccacag ctatttcaac ttgtctggtg atttcacgca gacgattgac
     7741 cgtcatgtct tccaactaaa cacagagggc atttactcaa tcgctcctga cggtgttcct
     7801 gccaaaactc cagaagccaa ccgtgatgtg gtcaaacaca tctacaatgg taccttgttg
     7861 aaggatatct ttgcagaaga agatgagcaa atccagctgg catcaggttt ggatcaccca
     7921 tttgcccttc ctgcaggcca tgacaatgct ggattccttt atgaccaaaa ttcaggtcgc
     7981 ttcctgcttt tcaagacaga agctccttgc tttgtggtct acacagcaaa ctttgtggat
     8041 gaaagtgtca tcataggagg tcagccaatg ctacagcaca atgggattgc tcttgaagcg
     8101 caagctttac cagatgccat tcacagtgac cttaaaggcc aagtcattct taaagctggt
     8161 caaaccttca ccagtaagac acgttatgaa cttgttgtga aataaaaaag ctggtcaaaa
     8221 agtctttaaa attaaaataa cgcatattgt caggtgatga aaagtttgat aatatgcgtt
     8281 ttattatgaa atgatttact ttatattatc ctgaaatggt gttatttttg agttatctgg
     8341 cttgactttc ttgatggaaa ttatataata gttgtaacaa taataattac aacgagaaaa
     8401 gaggaccatt tatgacctat gaatacaaga gtcacattta tttggcagag acagttttaa
     8461 atgtaaagga tttggccagt caaacagcct tttatcagca agttatcggt ttagaaatct
     8521 tatctcaaac tgagacagag tccattctag gcattggtgg aaaagtcttg gtacagctga
     8581 ttcaagcaca agagagcgga gaagtgaggg aacattatgg tctttaccat ttggccattc
     8641 tcttgcccac acgcaaggct ttggctgatg tcttgaaaca cctgacagat ttacagattc
     8701 ctcttgtcgg cggtgcagac catggttaca gtgaagctat ttacttggaa gatttggagg
     8761 gaaatggcat tgaactctat cgagataagc cagtttccac atgggatatt cgagaagatg
     8821 gacgcattat cggagtgacg gaggctcttg cggcgcagga catttatgag ttgggggaaa
     8881 gagtagagcc ctttatcctg gcagaaggta cgagaatggg gcatattcat ctttccgtca
     8941 aggatagtcg aaagtccagc cagttttatc aaaaggtgtt agggctagag aataaattca
     9001 gtgtgcctgg cgctagttgg attgcggctg gagattacca tcatcattta gcagttaacg
     9061 aatggagagg aaaaggtctg gcttcgcgta agcaaggctt accaggttta gcctactatg
     9121 tcatcgaagt cgcacataaa aaagaactgt taacgattgc ccaacgagca caagaagttg
     9181 acgcaccaat caaatggatg acatcgagca aattggaaat cacagactca gatggcatcg
     9241 tgacccgtat tcgtttagca aggtaaaatt aggaattttt aataagttat aagtattgct
     9301 agtttgcttt ttgaacggtc tttggtaaca gaactgcgtg agctggatgg gtgagaattt
     9361 tgacaaagaa gctgattaga gatataatga agaaaaatcg agcaaggaaa aagaaatgac
     9421 aaattcaaag tatattactc gtttgaaacg ttcagagggc cagttgcgtg ggattcaaaa
     9481 gatgattgaa ggagatcgtg actgtgctga tattgtgacg caactgacag cagtgaaatc
     9541 tagtgtagag cgcgtgattg agatgataat taccgaaaat cttactgaat gtatcaatca
     9601 gcccctagat gattctgaag ctcaaaagga acgcctagag aaggctatct gatacttgat
     9661 taaacggaaa taaatgaatg ttttgagacg atatgtgcaa aggtctatta actattagaa
     9721 tagtttaatg taagtcaaaa gaagttgtaa aaaatcttta aaattaaaaa acgcatagta
     9781 tcaggtattg aaatgtactg ccccccaaaa gttagatttt ttctgtctaa cttttggggg
     9841 gcagtacata aaaaccttga tagtatgcgt tttattgtgg ggagacttat actcttcgaa
     9901 aatctcttca aaccacgtca gtttcacctt gccgtagata tggttactga cttcgtcagt
     9961 tctatccaca acctcaaaac agtgttttga gctgacttcg tcagttccat ccacaacctc
    10021 aaaacagtgt tttgagctga cttcgtcagt tccatccaca accttaaaac agtgttttga
    10081 gcagcctgcg gctaacttcc tagtttgcac tttaattttc attgagtatt acttcatctt
    10141 ctcatgaaat tgagtttttg cccagctcct cttgattatt ctacttgacc gggccaagta
    10201 ttcataccac cttctacgtt ggtaacggtg aggccttggg agctgagaaa ttggcaggta
    10261 gatgcagagc ggactcctcc ttgacaaata acatagtatt catggtcagg tttgagttct
    10321 ttgtagcctt gctctaaggt acttaatgga agatttttgg cacctggtgc atgtcctgct
    10381 tggaattcat gtgcttcacg gacatcgata agttctagat tttcattttg gtattttcca
    10441 taaaagtcag ccatgctgat actagtttcc atattctact ccttctgggt tagccatttt
    10501 gtatagtgaa taagcgccgt caaggttttg gacggtaaat cctgcttgct tgaggatagg
    10561 ctctgcgata tagctgcgca aaccactgtg gcagctgacg atgtaggctt gttttttgtc
    10621 cagttcatca gcttcctcct tgacactttg tcagtttttg atacaatagt acaaaattag
    10681 aggaggtggg ctatgattca gaaacatgcg attcccattt tagagtttga tgataatcct
    10741 caggcggtta tcatgcccaa tcacgagggg ttggatttgc agttgccaaa gaagtgtgtc
    10801 tatgcttttt taggtgagga gattgaccgc tatgcgaggg aagtaggggc gaactgtgtt
    10861 ggcgaattcg tttctgctac caagacctat ccagtctatg tcatcaacta caagggtgag
    10921 gaggtctgtc tggctcaggc tcctgttggc tccgctccag cagcccagtt tatggattgg
    10981 ttgattggct atggtgtgga gcagattatc tctactggga cctgtggtgt cctagctgat
    11041 atagaggaaa atgcctttct agtccctgtt cgcgctttgc gagatgaggg agccagttac
    11101 cactatgtgg caccttgtcg ttatatggaa atgcagccag aggctattgc tgctattgag
    11161 gaagttttgg aagacagagg gattccttat gaagaagtca tgacctggac gacagacggt
    11221 ttttaccgag aaacggctga aaaggtggct tatcgtaagg aagaaggctg tgctgttgtg
    11281 gagatggagt gttctgctct tgcggcagta gctcaattgc gtggggttct ctggggtgaa
    11341 ttgttgttca cagcagattc tctagcggac ttggaccagt acgacagtcg tgactggggc
    11401 tcggaagctt ttaataaggc gctagaactg agtttagcaa gtgttcacca cctttagttg
    11461 tactggcaaa ggatttgttt tatcataaaa tgtctagctc atacttttca aaaatatgtt
    11521 taaacgaggt caccttcctc ttgtcctagg catgttgagg ttgggaaaaa tctttaaaat
    11581 cagaaaaacg tatcatatca ggtgatgaaa actttgatac tatgcgtttt atgttggtaa
    11641 gatttagagt gagatgaaat gatactcttc gaaaatctct tcaaaccagg tcagcttcac
    11701 cttgccgtag gtatatgtta ctgacttcgt cagtcttatc cggcaacctc aaaacggtgt
    11761 tttgagctga cttcgtcagt tctatccaca acctcaaaac agtgttttga gcaacctgtg
    11821 actagctttc taagcgatgc cttggttctc attgcctata atcaaaaaga gaaattttct
    11881 cctgaaaagc atatagagta gctggcgtta aaagctcctg tcttgctttt ttgacctata
    11941 gtcacatcta tcaagtattg ttcttgccta agctatcaat aaaaaggtgg cattttttag
    12001 gcttggtgtt agtagatttt gccttatcct atctaagtca tttcgagctt tttatggtac
    12061 aatggaaaca tgttattcaa attatctaag gaaaaaatag agctaggctt atctcgttta
    12121 tcgccagccc gtcgtatttt tttgagtttt gccttggtca ttttactagg ctctcttctt
    12181 ttgagcttgc cctttgtcca agttgaaagc tcacgagcga cttattttga tcatcttttc
    12241 actgctgtct ctgcagtctg tgtgacaggt ctctcaaccc ttccagtagc tcacacctat
    12301 aatatatggg gccaaataat ctgtttgctc ttgattcaga tcggtggtct agggctcatg
    12361 acctttattg gggttttcta tatccagagc aagcaaaagc ttagtcttcg tagccgtgca
    12421 actattcagg atagttttag ttatggagaa actcgatctt tgagaaagtt tgtctattct
    12481 atttttctca cgaccttttt ggttgagagc ttgggagcta ttttgcttag ttttcgcctt
    12541 attcctcaac ttggctgggg acgtggtctt tttagttcca tttttctagc gatctcagcc
    12601 ttctgtaatg ccggttttga taatttaggg agcaccagtt tatttgcttt tcagaccgat
    12661 ttactggtca atctggtgat tgcaggcttg attattacag gcggccttgg ttttatggtc
    12721 tggtttgatt tggctggtca tgtaggaaga aagaaaaaag gacgtctgca ctttcatacg
    12781 aagcttgtat tattattgac tataggtttg ttgttatttg gaacggcaac tactctcttt
    12841 cttgagtgga acaatgctgg aacgattggc aatctccctg ttgccgataa ggttttagtt
    12901 agcttttttc aaacagtgac gatgcgaaca gctggctttt ctacgataga ttatactcag
    12961 gctcatcctg tgactctttt gatttatatc ttacagatgt ttctaggtgg ggcacctgga
    13021 ggaacagctg ggggactcaa gattacgaca ttttttgtcc tcttggtctt tgcacgaagt
    13081 gagcttctag gcttgcctca tgccaatgtt gcgagacgaa cgatcgcgcc gcgaacggtt
    13141 caaaaatcct ttagtgtctt tattatcttt ttgatgagct tcttgatagg attgattctg
    13201 ctagggataa cagccaaagg caatcctccc tttatccacc tcgtatttga aaccatttca
    13261 gctcttagta cagttggtgt aacggcaaat ctgactcctg accttgggaa attggctctc
    13321 agtgttatca tgccacttat gtttatggga cgaattggtc ccttgacctt gtttgttagc
    13381 ttggcagatt accatccaga aaagaaagat atgattcact atatgaaagc agatattagt
    13441 attggttaag aaaggaaaga gcatgtcaga tcgtacgatt ggaattttgg gcttgggaat
    13501 ttttgggagc agtgtcctag ctgccctagc caagcaggat atgaatatta tcgctattga
    13561 tgaccacgca gagcgcatca atcagtttga gccagttttg gcgcgtggag tgattggtga
    13621 catcacagat gaagaattat tgagatcagc agggattgat acctgcgata ccgttgtagt
    13681 cgcgacaggt gaaaatctgg agtcgagtgt gcttgcggtt atgcactgta agagtttggg
    13741 ggtaccgact gttattgcta aggtcaaaag tcagaccgct aagaaagtgc tagaaaagat
    13801 tggagctgac tcggttatct cgccagagta tgaaatgggg cagtctctag cacagaccat
    13861 tcttttccat aatagtgttg atgtctttca gttggataaa aatgtgtcta tcgtggagat
    13921 gaaaattcct cagtcttggg caggtcaaag tctgagtaaa ttagacctcc gtggcaaata
    13981 caatctgaat attttgggtt tccgagagca ggaaaattcc ccattggatg ttgaatttgg
    14041 accagatgac ctcttgaaag cagataccta tattttggca gtcatcaaca accagtattt
    14101 ggatacccta gtagcattga attcgtaaag agggatgacc cctctttttt gatgcccaag
    14161 atggcaaata gagacagaag ccccttgtct tctagtaaaa gttcttcaaa ggctggactt
    14221 tatggtaaaa tagaaagaag tgacaagaga gagtaatact caatgaaaat caaagatcaa
    14281 actaggaaac tagctacggg ctgctcaaaa cagtgttttg aggttgcaga tagaactgac
    14341 gaagtcagta acatctatac ggcaaggcga cgttgacgcg gtttgaagag attttcgaag
    14401 agtataagaa aaaatcagcc ccctaaagga gcagattatg aagttattgt ctatcgccat
    14461 ttctagctat aatgcagcag cctatcttca ttactgtgtg gagtcgctag tgattggtgg
    14521 tgagcaagtt gggattttga ttatcaatga cgggtctcag gatcagactc aggaaatcgc
    14581 tgagtgttta gctagcaagt atcctaatat cgttagagcc atctatcagg aaaataaagg
    14641 ccatggcggt gtggtcaatc gcggcttggc agaggcttct gggcgctatt ttaaagtagt
    14701 tgacagtgat gactggtgga tcctcgtgcc tacttgaaaa ttcttgaaac cttgcaggaa
    14761 cttgagagca agggtcaaga gatggatgtc tttgtgacca attttgtcta tgaaaaggaa
    14821 ggtcagtctt gtaagaagag tatgagttac gattcagtct tgcctgttcg gcagattttt
    14881 ggctgggacc aggtcggaaa tttctccaaa ggccagtata tcatgatgca ctcgctgatt
    14941 tatcggacag atttgttgcg tgctagccag ttctaattgc ctgaacatac tttttatgtc
    15001 gataatctct ttgtctttac gccccttcag caggtcaaga ccaggtacta tctgcctgtc
    15061 gatttctatc gttatttgat taggcgtgag gaccagtctg tcaatgagca agtgatgatt
    15121 aagtgcattg accagcaact caaggtcaat cgactcttgg tagaccagct tgatttgtcc
    15181 caagtgagtc atcctaaaat gcgagaatat ctgctgaatc atattgaaat cacgacggtg
    15241 atttccagta ccctgctcaa ccgatctgaa acagcggagc atctggcaaa aaaacgccaa
    15301 ttgtggacct atattcagca ggaaaatcca aaagtctttc aggctattcg taagaccatg
    15361 ttgagccgtt tgaccaaaca ttctgtcttg ccagatcgca aactgtccaa tgtcgtctat
    15421 caaatcacca aatctgttta tggatttaat taatataagt gttttataag agggatttaa
    15481 gaaaaatttt aactttttct tagtcctttt taatttcagg agattatact agagtcatca
    15541 aataaagaaa gactctaagg agaatcctat gaaattcaat ccaaatcaaa gatatactcg
    15601 ttggtctatt cgccgtctca gtgtcggtgt tgcctcagtt gttgtggcta gtggcttctt
    15661 tgtcctagtt ggtcagccaa gttctgtacg tgccgatgtg gtcaatccga ccccaggtca
    15721 agtcttacct gaagagacat cgggaacgaa agagggtgac ttatcagaaa aaccaggaga
    15781 caccgttctc actcaagcga aacctgaggg cgttactgga aatacgaatt cacttccgac
    15841 acctacagaa agaactgaag tgagcgagga aacaaactct tctagtctgg atacactttt
    15901 tgaaaaagat gaagaagctc aaaaaaatcc agagctaaca gatgtcttaa aagaaactgt
    15961 agatacagct gatgtggatg ggacacaagc aagtccagca gaaactactc ctgaacaagt
    16021 aaaaggtgga gtgaaagaaa atacaaaaga cagcatcgat gttcctgctg cttatcttga
    16081 aaaagctgaa gggaaaggtc ctttcactgc cggtgtaaac caagtaattc cttatgaact
    16141 attcgctggt gatggtatgt taactcgtct attactaaaa gcttcggata atgctccttg
    16201 gtctgacaat ggtactgcta aaaatcctgc tttacctcct cttgaaggat taacaaaagg
    16261 gaaatacttc tatgaagtag acttaaatgg caatactgtt ggtaaacaag gtcaagcttt
    16321 aattgatcaa cttcgcgcta atggtactca aacttacagc gctacagtca atgtctatgg
    16381 taacaaagac ggtaaaccag atttggacaa catcgtagc
//
LOCUS       DAVMEI010000028        15759 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVMEI010000028 DAVMEI010000000
VERSION     DAVMEI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.028"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            103..1128
                     /gene="trpS"
                     /locus_tag="VYQ24_000734"
     CDS             103..1128
                     /gene="trpS"
                     /locus_tag="VYQ24_000734"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1967903.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1280..2758
                     /gene="guaB"
                     /locus_tag="VYQ24_000735"
     CDS             1280..2758
                     /gene="guaB"
                     /locus_tag="VYQ24_000735"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1967904.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2809..3906)
                     /gene="recF"
                     /locus_tag="VYQ24_000736"
     CDS             complement(2809..3906)
                     /gene="recF"
                     /locus_tag="VYQ24_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1967905.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(3909..4277)
                     /gene="yaaA"
                     /locus_tag="VYQ24_000737"
     CDS             complement(3909..4277)
                     /gene="yaaA"
                     /locus_tag="VYQ24_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1967906.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4436..5686
                     /locus_tag="VYQ24_000738"
     CDS             4436..5686
                     /locus_tag="VYQ24_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1967907.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5683..6966
                     /locus_tag="VYQ24_000739"
     CDS             5683..6966
                     /locus_tag="VYQ24_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1967908.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            7008..7829
                     /locus_tag="VYQ24_000740"
     CDS             7008..7829
                     /locus_tag="VYQ24_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1967909.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKISFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7840..8385
                     /gene="pgsA"
                     /locus_tag="VYQ24_000741"
     CDS             7840..8385
                     /gene="pgsA"
                     /locus_tag="VYQ24_000741"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1967910.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8382..9209
                     /locus_tag="VYQ24_000742"
     CDS             8382..9209
                     /locus_tag="VYQ24_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1967911.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRREWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9194..10033
                     /locus_tag="VYQ24_000743"
     CDS             9194..10033
                     /locus_tag="VYQ24_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1967912.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10026..10820
                     /locus_tag="VYQ24_000744"
     CDS             10026..10820
                     /locus_tag="VYQ24_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1967913.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10879..11697
                     /gene="mreC"
                     /locus_tag="VYQ24_000745"
     CDS             10879..11697
                     /gene="mreC"
                     /locus_tag="VYQ24_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1967914.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11697..12191
                     /gene="mreD"
                     /locus_tag="VYQ24_000746"
     CDS             11697..12191
                     /gene="mreD"
                     /locus_tag="VYQ24_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1967915.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12285..13463
                     /locus_tag="VYQ24_000747"
     CDS             12285..13463
                     /locus_tag="VYQ24_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1967916.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13687..14466
                     /gene="rpsB"
                     /locus_tag="VYQ24_000748"
     CDS             13687..14466
                     /gene="rpsB"
                     /locus_tag="VYQ24_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1967917.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14545..15585
                     /locus_tag="VYQ24_000749"
     CDS             14545..15585
                     /locus_tag="VYQ24_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1967918.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTCVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
BASE COUNT         4940 a         2490 c         3374 g         4955 t
ORIGIN      
        1 tacacaaatc tgtaaaaagc aaggctgatt tattttgata aattacggtt atttcattaa
       61 aaaaatgcta taattgaaag gattatatcg aaggagaaca aaatgactaa acccattatt
      121 ttaacaggag accgtccaac aggaaaattg catattggac attatgttgg aagtctcaaa
      181 aatcgagtat tattacagga agaggataag tatgatatgt ttgtgttctt ggctgaccaa
      241 caagccttga cagatcatgc caaagatcct caaaccattg tagagtctat cggaaatgtg
      301 gctttggatt atcttgcagt tggattggat ccaaataagt caactatttt tattcaaagc
      361 cagattccag agttggctga gttgtctatg tattatatga atctagtttc gttagcacgt
      421 ttggagcgaa atccaacagt caagacagag atttctcaga aaggatttgg agaaagcatt
      481 ccgacaggat tcttggtcta tccaatcgct caagcagctg atatcacagc tttcaaggct
      541 aattatgttc ctgttgggac agatcagaaa ccaatgattg agcaaactcg tgaaattgtt
      601 cgttctttta acaatgcata taactgtgat gtcttggtag agccggaagg tatttatcca
      661 gaaaatgaga gagcagggcg tttgcctggt ttagatggaa atgctaaaat gtctaaatca
      721 ctaaataatg gtatttattt agctgatgat gcggatactt tgcgtaaaaa agtaatgagt
      781 atgtatacag atccagatca tatccgcgtt gaggatccag gtaagattga gggaaatatg
      841 gttttccatt atctagatgt ttttggtcgt ccagaagatg ctcaagaaat tgctgatatg
      901 aaagaacgtt atcaacgagg tggtcttggt gatgtgaaga ccaagcgtta tctacttgaa
      961 atattagaac gtgaactggg tcctattcgt gagcgccgta ttgaatttgc taaggatatg
     1021 ggagaagttt ataatatgat tcaaaaaggt agtgaaagag cgcgtgaagt tgcgggtcaa
     1081 accctatctg aggtaaaagg ggcaatggga cttcattact ttaactaagt ttattttaca
     1141 agaaactatc ttttctatct cgaagaaaag atagtttttt atttacccct gtaacatttg
     1201 acaaattttt atagaatggt atgatagata caatataaaa agagtcaagc tcaaaaagaa
     1261 agaaaagagg aaacttcgaa tgtctaattg ggacactaaa tttttgaaaa aaggttttac
     1321 ctttgatgat gtattgctta ttccagctga aagtcatgtg ttgcctaacg atgcagattt
     1381 aacaactaaa ttggcagata atttgacttt aaatatccca attattaccg ctgccatgga
     1441 cacagttaca gagagtcaaa tggccattgc tattgctcgt gcaggcggtc tcggagttat
     1501 ccataaaaac atgtcaattg ctcaacaagc agacgaggtt cgtaaggtaa aacgttctga
     1561 aaatggagtt attattgatc cgttcttctt gacgcctgaa catacaattg ctgaagcaga
     1621 tgagcttatg ggtcgttacc gcatcagtgg tgttccagtt gttgaaacac ttgaaaatcg
     1681 taaattggtt ggtattttga caaaccgaga tcttcgtttt atttcagatt ataatcaacc
     1741 aatttcaaac catatgacta gtgaaaatct tgttactgct cctgtgggta cggatcttgc
     1801 aacggctgag agtattcttc aagagcatcg tattgaaaaa cttccgttgg tcgatgaaga
     1861 aggcagtctt tctggtttga tcactatcaa agatattgaa aaagttattg agtttccaaa
     1921 tgcggctaaa gatgagtttg gtcgtcttct agttgcaggt gcagtaggtg ttacttcaga
     1981 tacatttgaa cgtgcagagg ctctttttga ggcaggagcg gatgcgattg ttattgatac
     2041 tgcacatggt cattctgcag gtgtcttgcg taaaattgcc gagattcgtg ctcatttccc
     2101 agatcggact ttgattgctg gaaatattgc tactgctgaa ggtgcacgtg ccctttatga
     2161 agcgggtgta gacgttgtta aggttggtat tggaccaggt tctatctgta ctactcgtgt
     2221 gattgctggt gttggtgttc cgcaagtaac agctatctac gatgctgcag ctgttgcgcg
     2281 cgaatatggt aaaacgatta ttgctgacgg tgggatcaag tattctggag atattgtaaa
     2341 agcacttgct gcaggtggaa atgctgttat gcttggatct atgtttgctg gaactgatga
     2401 agctccaggc gaaactgaaa tcttccaagg acgtaaattc aagacttacc gtggtatggg
     2461 atcaattgct gctatgaaga aaggttcaag cgaccgttat ttccaaggtt ctgtcaatga
     2521 agcaaacaag cttgttccag aaggaattga aggtcgtgtt gcttataaag gagcggcagc
     2581 tgatattgtt ttccaaatga ttggtggtat tcgctctggt atgggttact gtggtgcagc
     2641 taaccttaaa gaactacacg ataatgctca atttattgaa atgtctggtg ctggtttgaa
     2701 agaaagccat cctcatgatg tgcaaattac taatgaggca ccaaattatt ctatgtaaaa
     2761 aacaatgaaa agaactccag tgaaaacagg agttctttta caatgttgtc aatttccatt
     2821 tacagaagct ttaccatcct gaatagtgaa gatacttaga ttttctggca gattttgaag
     2881 atggtctaag cttgttgttg tgataaaggt ttggattgat tgagaaatcg tttctaataa
     2941 ttttaactgt ctagtgttgt caagttcact catcacatcg tcaagcagta atatcggaga
     3001 ttctgtggta atgctttcca ttaattcgat ttctgctaat tttatcgaga ggacgagact
     3061 acgatgttga ccttggcttc cgaaactagc atccatccca tttatataaa aagaaatgtc
     3121 atctcgatga ggaccgacac cagtattctt tttaaataaa tctctggatc tacttttttc
     3181 taaagcaatt ttgaaagatt cggataagtt ttgtttgtca gttatattga cagaagattg
     3241 ataggatatt gacaactctt cgatctgatt agagagttca aaatgtttct tacgcccaaa
     3301 tgattctagt ttttttatga aatctaagcg gtgattcatt acacgacatc cataatcaac
     3361 tagctgatca tctaacacag aaaggaatgt ttcatctatt ttttgatctg attttaggta
     3421 agtgtttctt tgctttagga tgtggttata attggttaag tcagataagt agattggctt
     3481 aatttgccca agttccatat caatgaattt tcgtcgaatc gaaggtgctc ctttaattag
     3541 ttgtaaatct tcaggagcaa ataagacaac attcatgtgt cctacataat ctgaaaggcg
     3601 tgcctgtttt aagtgattaa cttttgttac acggcctttt tgtgttagtt cgatttctag
     3661 gggaatggat ccagtttttt tctgaacgag acctgaaaga tgaagttgtt cctcatcaaa
     3721 atgaatgaga tttttatctg ttcgagttcg atgactacgc gttaaggcta aaaaatagat
     3781 agcctctaac atgtttgttt ttccttgtgc attacgtccc aaaaagacat tcaatttagg
     3841 attaaagtct attttcgttt ctttgtaatt acgaaaagtc ttgagagata gatgttgtag
     3901 ccacatgatt atctaccagg gaatcgtgga gcttgttggc ttttaggtga tgaagcaggt
     3961 ttcgatttgt cttttttaac tcccttattc atctttttga caagtttagc gatccgttct
     4021 ttttcaactt tatcagcttg gtattcatct tgttcttcag aagtaggttg tgtcaacaag
     4081 atgtcaatgt tcatgtcagg gatgtcaact ttatcgccaa tacgaagttt ttttccacga
     4141 cgactctcta attccctatt aaagtaaaca gaatgttcag agagaaatga tttaatagct
     4201 cctccgctat gtgtaattcc aagttctttg agtagtgctt ggagggtaat aaattcttca
     4261 aataatttgt attccataat tcacctcaat ctttggtatt ataccatatt ttcctaaaaa
     4321 tagtgagaga gacagcgaga aatgtaagcg atttttattt caaaagtgtt acagggaaca
     4381 agaaaatttc aggttttcgt gatataatag aagtctgtat ataaggaggt aaatcatgga
     4441 gttagtgcat ggaatttcaa cacattttat ccaatcaaaa aagtttaaaa caaacaaaat
     4501 taccgtgcgt tttaccgctc cattatccct tgatacgatt gcaggtcaca tgttgagtgc
     4561 aagtatgcta gagactgcta atcagatgta ccccacttct caagatttga ggagacactt
     4621 ggccagtcta tacggtacag atatgtcaac caattgtttc agaagagggc aaagccacat
     4681 tatagaattg acatttacct atgttcgtga tgagttttta agtaggaaaa acgtgctaac
     4741 ctctcagatt ttggaacttg taaaagaaac tcttttttca cccgtagtag ttgataatgg
     4801 gtttgatccg gccttatttg aaattgagaa aaaacaattg ctagcaagtt tagcagctga
     4861 tatggatgat tctttttatt ttgcacataa agaattggat aaattgtttt ttcatgatga
     4921 acgtcttcaa ttggaatata gtgatttacg aaatcgtatt ttagctgaaa ctccacaaag
     4981 ttcttattct tgtttccaag aatttttagc caatgatcga atagatttct ttttcctagg
     5041 tgattttaat gaggttgaaa ttcaaaatgt attagaatca tttggcttta aaggtcgaaa
     5101 aggagatgtg aaggttcagt attgtcaacc ttattctaat atccttcagg aaggtatggt
     5161 tcggaaaaat gtgggacaat ccattttgga attaggttat cattactgtt ctaaatatgg
     5221 tgatgagcaa catttaccca tgattgtaat gaatggttta cttggtggat ttgctcactc
     5281 taagctcttt acaaatgtcc gtgaaaatgc tggattagct tataccattt caagtgagct
     5341 tgatttattt agtggattct tgaggatgta tgctggtatc aatcgagaaa atcgtaacca
     5401 ggctcgtaaa atgatgaata atcaactgct tgatttaaaa aaaggttatt ttacagagtt
     5461 tgagttaaat cagaccaagg aaatgattcg ttggtcgttg ttactttctc aagataatca
     5521 atcttcattg attgaacgtg cttatcaaaa tgccttattt ggaaaatctt cagcagactt
     5581 taaaagttgg attgcaaagc ttgaacaaat tgacaaagat gctatttgta gagtagctaa
     5641 taatgtgaaa ctacaagcga tttactttat ggaaggaata gaatgacaaa ggttgttttt
     5701 gaagaaaaat actatccagc tgtaaaagaa atggtttatc gaactcgttt ggccaacgga
     5761 ttgacagttg ctcttttgcc taaaaaggaa tttaaagagg tttacgggag tgtcactgta
     5821 cagtttggtt cggtagatac gtttgtcaca gaagttgacg gagatgtaaa acaatatcct
     5881 ggaggaattg ctcattttct tgaacataaa ttatttgaga gagaagattc tagtgatttg
     5941 atgtcggctt ttacgagtct aggtgcagat agtaatgcct ttacaagctt tacaaaaaca
     6001 aactatcttt tttcagcaac ggattatttt ttagaaaatt tagatttact tgatgaattg
     6061 gtaacatcag cacactttac tgaagcttcc attctgacag agcaggatat tattcagcaa
     6121 gaacgagaaa tgtaccaaga tgatccagat tcgtgtttat tcttttcaac tttagcgaat
     6181 ttgtatcctg gtacaccttt agcaactgat atagttggaa gtgaggagtc catttcccaa
     6241 atcaatctaa ctaatttgca agaaaatttt acaaagtttt acaaacctgt aaacatgtct
     6301 ctgtttttag ttggtaattt tgatgtggag cgagtacagg actattttga aagcaaagaa
     6361 ctgaaagatt cagattttca ggaagtagca agagaaaagt tgtttttaca gcctgtaaag
     6421 ccaacagata gtatgagaat ggaagtatct tctcccaaac tagcgattgg agttagaggt
     6481 aagcgagaag tttctgaagc ggattgctat cgacatcata ttttattaaa attattgttt
     6541 gcaatgatgt ttggttggac ttcggatcgt tttcaaaaat gttatgaatc aggtaaaatt
     6601 gatgcgtcct tatctctgga agttgaaata acaagtcgct ttcattttgt catgttgaca
     6661 atggatacga aagagccagt tgctttgtct catcaattta ggaaggctat tcgtaatttt
     6721 acaaaggatt tagatattac agaggaacat ttagatatta tcaaaagaga gatgtttggc
     6781 gaatttttca gtagcatgaa ctctcttgaa tttattgcaa cgcaatatga tgcttttgaa
     6841 aatggtgaga caatttttga tttgccgaaa attttacagg aaattacttt agaggatgtc
     6901 cttgatgctg gacatcattt aatagatgat ggtgacatag ttgattttac aatattccca
     6961 tcgtagtaac ctattataat agacactaga aagaagggat gacaagtatg agaaaaaaaa
     7021 caattggaga ggttttacga ttagctagaa tcaatcaggg attgagttta gatgaattgc
     7081 agaaaaagac agaaatccag ttagatatgt tggaagcaat ggaagcagac gatttcgatc
     7141 aacttccaag tcctttttac acgcgttctt tcttgaaaaa atatgcatgg gctgttgagt
     7201 tagatgacca aattgttttg gatgcttatg attctgggag tatgattact tatgaggaag
     7261 tagatgttga tgaagatgag ttgacaggtc gtagacgttc aagtaagaaa aagaagaaaa
     7321 aaatatcatt tttaccttta ttttatttta tcctttttgc tttatcgatt ttaatttttg
     7381 tgacttatta tgtttggaac tatattcaaa ctcaaccaga ggagccttct ctttctaatt
     7441 acagtgtggt tcaatcaaca agttcaacta gctctgttcc ccactcctca agtagtagtt
     7501 cttctagtat agaatcagct ataagtgtat caggcgaagg aaatcatgta gaaatcgctt
     7561 ataagacaag taaggaaaca gttaaattgc aattggcagt ttcagatgtt acaagttggg
     7621 tcagtgtttc agaaagcgaa cttgagggcg gtgtaacctt atcgccaaag aagaaaagtg
     7681 cagaagcaac agttgcaact aaaagtcctg taacaattac gttaggtgtt gtaaaaggtg
     7741 ttgatttgac agtagataat cagactgttg atttatcgaa attaacagct cagactggac
     7801 aaatcactgt aacctttact aaaaattaag gaaaaacgaa tgaaaaaaga acaaattccc
     7861 aatctcttaa caataggtcg aattctcttt atacctattt ttatctttat tttaacgata
     7921 ggaaattcga tagagagtca tatagttgca gctattatct ttgctgttgc cagtattacc
     7981 gactatttag atggatattt agctcgtaaa tggaatgtgg tcagtaattt tggtaaattt
     8041 gcagatccta tggcggataa gttactagtt atgtcggctt ttattatgtt gattgagtta
     8101 ggtatggctc cggcttggat tgttgcagtg attatctgtc gtgagttagc tgtgacaggt
     8161 ttaaggcttt tattggttga aactggtgga acaattttag cagcagcaat gcctggaaaa
     8221 attaaaactt ttagtcagat gtttgccatt attttcttgc tattacattg gactttgctt
     8281 ggtcaagttc tactttatgt agccttattt ttcactatct actctggcta tgactatttc
     8341 aagggtagtg cctatgtatt taaagggaca tttggttcga aatgaaatca ataattgatg
     8401 taaaaaatct ttcttttcgc tataaagaaa atcagaacta ctacgatgtg aaggatatta
     8461 cgtttcacgt gaaacgtaga gaatggcttt cgattgtagg gcataatggt agtggtaaat
     8521 caacgacggt tcgattaatt gatggcttac tggaagcaga atccggagag attgtaattg
     8581 atggccaacg tttgactgag gaaaatgttt ggaatatacg tcgtcaaatc ggtatggttt
     8641 ttcaaaatcc agacaatcaa tttgttggag cgactgttga agatgatgtt gcttttggtt
     8701 tggaaaatca gggactttct cgtcaagaaa tgaaaaagag agtggaagaa gctctggctt
     8761 tagttggcat gttggacttt aaaaagagag agccagcgcg tctatcaggt ggccaaaagc
     8821 aacgtgtggc cattgcaggt gttgtagccc taagaccagc tattttaatc ttagatgaag
     8881 caacgagtat gttggatcct gaggggcgta gagaacttat tgggacagta aaaggaattc
     8941 gaaaagacta tgatatgacg gtcatttcta ttacccatga tttggaagaa gtcgccatga
     9001 gtgatcgcgt attggtcatg aaaaaagggg aaattgaatc aactagtagt ccaagggagc
     9061 ttttctctcg aaatgattta gatcaaattg gattagacga tccttttgct aatcaattaa
     9121 aaaaatcttt gagccagaat ggctatgatt tacctgaaaa ttatttgaca gaaagtgagc
     9181 tagaggataa gctatgggaa ttgctctaga aaatgtgaat tttacatatc aagaaggtac
     9241 tcccttagct tcagcagctt tgtcggatgt ttctttgacg attgaagatg gctcttatac
     9301 agctttaatt gggcacacag gtagtggtaa atcaactatt ttacaactct taaatggttt
     9361 attggtgcca agtcaaggga gtgtgagggt ttttgatacc ttaatcacct cgacttctaa
     9421 aaataaagat attcgtcaaa ttagaaaaca ggttggcttg gtatttcagt ttgctgaaaa
     9481 tcagattttt gaagaaacgg ttttgaagga cgttgctttt ggaccgcaaa attttggagt
     9541 ttctgaagaa gatgctgtga agactgcgcg tgagaaactg gctctggttg gaattgatga
     9601 atcacttttt gatcgtagtc cgtttgagct gtcaggggga caaatgagac gtgttgccat
     9661 tgcaggcata cttgccatgg agccagctat attagtctta gatgagccaa cagctggtct
     9721 agatcctcta gggagaaaag agttgatgac cctgttcaaa aaactccacc agtcagggat
     9781 gaccatcgtc ttggtaacgc atttgatgga tgatgttgct gaatatgcga atcaagtcta
     9841 tgtaatggaa aagggacgtt tagtaaaggg gggcaaacca agtgatgtct ttcaagacgt
     9901 tgtttttatg gaagaagttc agttgggagt acctaaaatt acggcctttt gtaaacgatt
     9961 ggctgataga ggcgtgtcat ttaaacgatt accgattaag atagaggagt tcaaggagtc
    10021 gctaaatgga tagtatgatt ttagggcgtt atatcccagg ggattcgatt gttcaccgat
    10081 tggatccacg tagcaaattg cttgctatga tgctactgat tttgattgtg ttttgggcta
    10141 ataacccctt gacgaatctg attcttttta tagcgacagg gatatttatt gccttgtcag
    10201 gagtatctct ttcatttttt attcagggct tgaagtctat gtttttcttg attgccttca
    10261 caactatttt tcaactattt ttcatttcta atgggaatgt tttatttgag ttttcgtttg
    10321 tgagaatcac ggattatgct ttgcaacaag ctgggattat tttttgtcgc tttgtattga
    10381 ttattttctt ttcaactttg ttaaccttaa caaccatgcc tttaagtttg gcatcagctg
    10441 tcgaagcttt attagcacct ttaaagcgtg tgaaagttcc agttcatgaa attggattga
    10501 tgctgtctat gagtttgcgt tttgtcccaa ccttgatgga tgatacgact cggattatga
    10561 atgcacagaa agctcgtgga gtggattttg gagaaggaag catcgttcaa aaagtaaagg
    10621 cgatgattcc cattttgatt cctctttttg cgacaagttt aaaacgtgca gattccttgg
    10681 ctatcgctat ggaagcgcgt ggctatcagg gtggaaaagg cagaagtcaa taccgacaat
    10741 tgaaatggac tctgaaggat acgctgacca ttcttgttat tctcgtactt ggttgttgtt
    10801 tatttttctt aaaatcttag tagctttcag gaattgataa aaagttactg taacagtttt
    10861 tgatataaag gtaggaatat gaaccgtttt aaaaaatcaa aatatgtcat tattgttttt
    10921 gtcactgttc tgcttgtgtc agctctctta gcgacgactt attcaagtac aattgtgaca
    10981 aaattaggag atggaatctc attggttgat agagttgtac aaaaaccttt tcagtggttt
    11041 gattctgtca aatcagattt ggctcatttg acacgaacat ataatgaaaa tgaaagtttg
    11101 aagaaacagc tttaccaatt agaagttaaa tcaaatgagg tggaaagttt aaagacagaa
    11161 aatgaacaac tgcgccaatt gcttgatatg aagtctaaat tgcaagccac aaagacttta
    11221 gcagcagatg ttattatgcg ttctccggta tcttggaagc aggagttgac cttagatgca
    11281 ggtagatcaa aaggtgcttc tgagaacatg ttagctattg caaatggtgg cttgattggg
    11341 agtgtttcaa aagtagagga gaactctact atagtcaacc ttctgacaaa tacggaaaat
    11401 gctgataaga tttctgttaa aattcaacat ggctctacta caatttatgg aattattatt
    11461 ggctatgaca aggaaaatga cgttcttaaa attagccaat taaatagtaa tagcgatatt
    11521 agtgcgggag ataaggtgac tactggtgga ttaggaaact ttaacgttgc tgatattcct
    11581 gttggtgaag tggttgccac aacgcatagt acagactatt tgacacgaga agtaactgtt
    11641 aaattgagtg cagatactca taatgtagat gtgatagaat tagtggggaa ttcataatga
    11701 gacagttgaa gcgagttgga gtatttttat tgcttccttt ctttgttcta attgacgccc
    11761 atattagcca gcttctgggc tcatttttcc cccatgtaca tttggctagt cattttcttt
    11821 ttctatttct cttatttgag acgatagaag tatcagagta tctctaccta gtctattgtt
    11881 ttgttatagg cttggtttat gatgtttact ttttccatct aatagggatt acaactctct
    11941 tatttatctt attgggagcc ttccttcata aattgaatag tgttattttg ttgaatcgtt
    12001 ggacaagaat gctagctatg attgtgctga cattcctgtt tgaaatgggt agttatcttt
    12061 tggcttttat ggtagggttg acagtagata gcatgtcgat ttttatagtc tatagcttgg
    12121 taccgacgat gattttaaat tttttatgga ttactgtttt tcaatttatt tttgaaaaat
    12181 attatctata agaacgacat ataaatgtaa caaaggcgta atatttatta ggcctttttt
    12241 tggtatacta gtattgtctt taaaagaagg agtatctacg taatatgaag aaaaaaatct
    12301 tagcgtcact tttattaagt acagtaatgg tttctcaagt agctgtttta acaactgcgc
    12361 atgcagaaac gactgatgac aaaattgctg ctcaagataa taaaattagt aacttaacag
    12421 cacaacaaca agaagcccaa aaacaagttg accaaattca ggagcaagta tcagctattc
    12481 aagctgagca gtctaacttg caagctgaaa atgatagatt acaagcagaa tctaagaaac
    12541 tcgagggtga gattacagaa ctttctaaaa acattgtttc tcgtaaccaa tcgttggaaa
    12601 aacaagctcg tagtgctcaa acaaatggag ccgtaactag ctatatcaat accattgtaa
    12661 actcaaaatc aattacagaa gctatttcac gtgttgctgc aatgagtgaa atcgtatctg
    12721 caaacaacaa aatgttagaa caacaaaagg cagataaaaa agctatttct gaaaaacaag
    12781 tagcaaataa tgatgctatc aatactgtaa ttgctaatca acaaaaattg gctgatgatg
    12841 ctcaagcgtt gactacgaaa caggcagaac taaaagctgc tgaattaagt cttgctgctg
    12901 agaaagcgac agctgaaggg gaaaaagcaa gtctattaga gcaaaaagca gcagctgagg
    12961 cagaggctcg tgcagctgcg gtagcagaag cagcttataa agaaaaacga gctagccaac
    13021 aacaatcagt acttgcttca gcaaacacta acttaacagc tcaagtgcaa gcagtatctg
    13081 aatctgcagc agcacctgtc cgtgcaaaag ttcgtccaac atacagtaca aacgcttcaa
    13141 gttatccaat tggagaatgt acatggggag taaaaacatt ggcaccttgg gctggagact
    13201 actggggtaa tggagcacag tgggctacaa gtgcagcagc agcaggtttc cgtacaggtt
    13261 caacacctca agttggagca attgcatgtt ggaatgatgg tggatatggt cacgtagcgg
    13321 ttgttacagc tgttgaatca acaacacgta tccaagtatc agaatcaaat tatgcaggta
    13381 atcgtacaat tggaaatcac cgtggatggt tcaatccaac aacaacttct gaaggttttg
    13441 ttacatatat ttatgcagat taatttacag agggactcga atagagccct cttttcaggt
    13501 tttaccgtga caatccctat taaaaattat atcaaaatag cttgaaaata ttggaaaagt
    13561 atggtaaaat gaaaattgtc gtgtgaacga taatactcat tcttgatgaa ttgtgaagca
    13621 gttgcccttg ggtcgttttg cgagttgaag tcaagaagag gaaaaaaaca aaaaggagaa
    13681 atactcatgg cagtaatttc aatgaaacaa cttcttgagg ctggtgtaca ctttggtcac
    13741 caaactcgtc gctggaatcc taagatggct aagtacatct ttactgaacg taacggaatc
    13801 cacgttatcg acttgcaaca aactgtaaaa tacgctgacc aagcatacga cttcatgcgt
    13861 gatgcagcag ctaacgatgc agttgtattg ttcgttggta ctaagaaaca agcagctgat
    13921 gcagttgctg aagaagcagt acgttcaggt caatacttca tcaaccaccg ttggttgggt
    13981 ggaactctta caaactgggg aacaatccaa aaacgtatcg ctcgtttgaa agaaattaaa
    14041 cgtatggaag aagatggaac tttcgaagtt cttcctaaga aagaagttgc acttcttaac
    14101 aaacaacgtg cgcgtcttga aaaattcttg ggcggtatcg aagatatgcc tcgtatccca
    14161 gatgtgatgt acgtagttga cccacataaa gagcaaatcg ctgttaaaga agctaaaaaa
    14221 ttgggaatcc cagttgtagc gatggttgac accaatactg atccagatga tatcgatgta
    14281 atcatcccag ctaacgatga cgctatccgt gctgttaaat tgatcacagc taaattggct
    14341 gacgctatta tcgaaggacg tcaaggtgag gatgcagtag cagttgaagc agaatttgca
    14401 gcttcagaaa ctcaagcaga ttcaattgaa gaaatcgttg aagttgtaga aggtgacaac
    14461 gcttaattta tacaaatagt aattacctag gagggcgggg cttagcccgg ctctcctatt
    14521 ttcaaaaaat ataggagaat taaaatggca gaaattacag ctaaacttgt aaaagagttg
    14581 cgtgaaaaat ctggtgccgg tgttatggac gctaaaaaag cgcttgtaga aacagacggt
    14641 gacatcgaaa aagcgattga attgcttcgt gaaaaaggta tggctaaggc agctaagaaa
    14701 gctgaccgtg ttgctgcaga aggtttgact tgtgtttatg ttaacggtaa tgttgcagca
    14761 gttattgaag taaacgctga aactgacttc gttgcaaaaa acgctcaatt cgttgaattg
    14821 gtaaatacta cagctaaagt cattgctgaa ggaaaacctg ctaacaatga agaagctctt
    14881 gctttgataa tgccttcagg tgaaactctt gaagctgcat acgtatctgc aacagcaact
    14941 atcggagaga aaatctcatt ccgtcgcttt gcattgattg aaaaaacaga cgcacaacac
    15001 tttggagcat accaacataa cggtggacgt atcggtgtta tttcagttgt tgaaggtgga
    15061 gacgaagcac ttgctaaaca attgtcaatg cacatcgcag cgatgaaacc aacagttctt
    15121 tcttacaaag aattggatga gcaattcgtt aaagatgagt tggcacaatt gaatcacgtt
    15181 atcgaccaag acaacgaaag ccgtgcaatg gtcaataaac cagctcttcc acacttgaag
    15241 tatggatcaa aagctcaatt aactgatgat gttattgctc aagctgaagc tgacatcaaa
    15301 gctgaattgg ctgcagaagg caaaccagaa aaaatctggg acaaaattat tccaggtaaa
    15361 atggatcgct tcatgcttga taacactaaa gttgaccaag cttacacact tcttgcacaa
    15421 gtttacatca tggatgacag caagacagtt gaagcatacc ttgaatcagt taacgcttcg
    15481 gtagttgagt ttgctcgctt tgaagttggt gaaggtatcg agaaagctgc aaacgacttc
    15541 gaagctgaag ttgcagctac aatggcagca gccttgaata actaataata gaaaggaagc
    15601 aaattgcttc cttttttgta tagggcttag atttttcctg aaattgtttt ttccccagtc
    15661 tcactcgatt agaataatta aaacaaaaag ccctataata agggctaagg gcatttagga
    15721 gactatactt caaattcata gtaagcgcgt tcataacaa
//
LOCUS       DAVMEI010000029        15641 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVMEI010000029 DAVMEI010000000
VERSION     DAVMEI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.029"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            24..1442
                     /locus_tag="VYQ24_000750"
     CDS             24..1442
                     /locus_tag="VYQ24_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1967919.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGILL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFKDLKTIFGAALGSEGLGALISDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTSIGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLASLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQAK
                     DVHVMIWILDALFILNYISMAL"
     gene            1547..2254
                     /locus_tag="VYQ24_000751"
     CDS             1547..2254
                     /locus_tag="VYQ24_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1967920.1"
                     /translation="MIKKNIWDEVLNRGKWLIIFLVGLLFSQFPLALATFLTSRKFPL
                     LQTGLLVGALSLVVLTVFIIGARKTQLASFGLSFFKAKDLARLALSYLVILAFNILGV
                     VLLHLMNETTTSNQSNINDLVQNSSLISSFFLLLLIAPICEEILCRGVIPKKLFRGKE
                     NVGYIVGAIIFALLHLPTNLPSLLIYGGMSTVLTWTVYKTQRLEMSILLHMIVNGIFF
                     CLLALVVILSRTLGISV"
     gene            2348..3328
                     /gene="folP"
                     /locus_tag="VYQ24_000752"
     CDS             2348..3328
                     /gene="folP"
                     /locus_tag="VYQ24_000752"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1967921.1"
                     /translation="MMKSLEGVTDMSTKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMARVVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQAFTEEELADFETLPIEDLMDAFFERALARANQAGIAQENILLDLGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            3330..4652
                     /locus_tag="VYQ24_000753"
     CDS             3330..4652
                     /locus_tag="VYQ24_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1967922.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTIAFLKNMLEKLGLRVGVFSSPYLIHYTDQISINGESIPEARLEALMADY
                     QSLLEGEAVANLQSTTEFEIITALAYDYFAAEQVEVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVSHQKSVVTGEVFDYTSVVRQGRFQTGLLGLHQIENAGMAIALLDTFCQE
                     HGRELASNDLLGQALEETSWPGRLEVVSRDPLMILDGAHNPHAIKALLATLQERFADY
                     RKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFDDSRATDESVLEEAAKSRNLNYQG
                     WQDFLEQKLTDKKEEKKTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            4633..5187
                     /gene="folE"
                     /locus_tag="VYQ24_000754"
     CDS             4633..5187
                     /gene="folE"
                     /locus_tag="VYQ24_000754"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1967923.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMEYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            5346..6158
                     /gene="folK"
                     /locus_tag="VYQ24_000755"
     CDS             5346..6158
                     /gene="folK"
                     /locus_tag="VYQ24_000755"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1967924.1"
                     /translation="MDQLQIKDLEIFAYHGLFPSEKELGQKFVVSAILFYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETTEDLIETVAYKLVERTFEAYPLVQEMKLELKKPWA
                     PVHLPLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRNQGIHILKESSVLTT
                     EPWGGVEQDSFANQVVEVETWLPAPVLLESLLAIESEMGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYNALKQ"
     gene            complement(6204..6521)
                     /locus_tag="VYQ24_000756"
     CDS             complement(6204..6521)
                     /locus_tag="VYQ24_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1967925.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            6878..7324
                     /gene="rplM"
                     /locus_tag="VYQ24_000757"
     CDS             6878..7324
                     /gene="rplM"
                     /locus_tag="VYQ24_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1967926.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            7344..7736
                     /gene="rpsI"
                     /locus_tag="VYQ24_000758"
     CDS             7344..7736
                     /gene="rpsI"
                     /locus_tag="VYQ24_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1967927.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(7998..8420)
                     /locus_tag="VYQ24_000759"
     CDS             complement(7998..8420)
                     /locus_tag="VYQ24_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1967928.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            8567..8743
                     /locus_tag="VYQ24_000760"
     CDS             8567..8743
                     /locus_tag="VYQ24_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967929.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            9144..11357
                     /locus_tag="VYQ24_000761"
     CDS             9144..11357
                     /locus_tag="VYQ24_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1967930.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     NSLDYIETSNVLDIMTDYFHLHFNKGKFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNKNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQVAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GILDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSDKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(11579..12055)
                     /locus_tag="VYQ24_000762"
     CDS             complement(11579..12055)
                     /locus_tag="VYQ24_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1967931.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            12278..15481
                     /locus_tag="VYQ24_000763"
     CDS             12278..15481
                     /locus_tag="VYQ24_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1967932.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
BASE COUNT         4794 a         2737 c         3464 g         4646 t
ORIGIN      
        1 taaaaattaa aaggagattg ataatggaca aattatttaa actaaaagag aacggtacag
       61 acgttcgtac agaggttctc gctggtttaa caactttctt tgcaatgagc tatattctct
      121 ttgtaaaccc acaaattctt tcgcaaacag gaatgcctgc tcagggtgta ttcctcgcaa
      181 cgattattgg tgcagttgct ggtaccctga tgatggcctt ctacgctaac ttaccttatg
      241 cccaagcgcc aggtatggga ctcaatgcct tctttacctt tacagttgta ttcggacttg
      301 gttattcttg gcaagaagcc ctagctatgg tcttcatctg tggaattatc tcattgatta
      361 ttaccttgac aaatgttcgt aaaatgatca ttgaatcgat tcccaatgct cttcgctcag
      421 ctatttcagc tggtatcggt gtcttccttg cctatgtagg gattaagaat gctggacttt
      481 tgaaatttac gattgatcca ggtaactata ctgttgtagg agaaggggct gacaaagctc
      541 aagcaacgat tgcagcaaac tcttcagcag ttccaggatt ggtcagcttt aataatccag
      601 ctgttttggt ggctcttgca ggactcgcta tcacaatttt ctttgtcatc aaaggaatta
      661 aagggggaat tctcctctct atcttgacaa caactgttct tgctattgca gttggtttgg
      721 tagatttgtc aagcatcgat tttgctaata accatgttgg tgcagctttt aaagatttga
      781 agacaatctt tggtgcagct cttggttcag aaggtttggg agctttgatt tcagatacag
      841 ctcgcttgcc tgaaactctg atggctattc ttgccttttc attgacagat atttttgata
      901 caattggtac cttgatcggt acaggtgaaa aagttggtat cgtagcgaca aatggtgaaa
      961 atcatcaatc agctaagtta gacaaggctc tttactctga cttgattggt acttcaattg
     1021 gtgccatcgc aggtacttcg aacgtaacga cttatgttga gtctgctgct ggtatcggtg
     1081 caggtggacg tactggtttg acagccttgg tcgtggcaat ctgttttgcg atctcaagct
     1141 tctttagccc acttctagcg attgtaccaa cagccgctac agctccaatc ttgattatcg
     1201 ttgggattat gatgttggct agcttgaaaa atatccattg ggacgatatg tctgaagcgg
     1261 ttcctgcttt cttcacatct atctttatgg gattcagcta ctctatcact caagggattg
     1321 cagttggttt cttgacttac actttgacta agcttgtcaa aggtcaagct aaagatgttc
     1381 atgtcatgat ttggattttg gatgctttgt ttatccttaa ctacatcagt atggcactat
     1441 aaatgctata atataaaaag gggttctccc ctttttatat tatagttact tgaaaattgc
     1501 ttcaaactag gtaggtgatt ttgattgaat ggtaggagta taattgatga taaaaaagaa
     1561 tatttgggat gaagtattaa acagggggaa gtggttaata atctttctag tagggctttt
     1621 gttttctcag tttcctttag ccctagcaac ttttttaact agcagaaaat ttccactgct
     1681 tcaaacggga ctattagtag gggccttatc tcttgtggtt ttaactgtat ttataatagg
     1741 tgctaggaaa acacagttgg ctagttttgg tttatcgttt ttcaaggcta aggatttggc
     1801 tagattggcc ttaagctatc tagtgattct tgcctttaat attcttggtg tggtcttact
     1861 gcatttgatg aatgaaacaa caaccagcaa tcaatcaaat attaatgatt tggtccagaa
     1921 tagttcctta atttccagtt ttttcttact gcttctaata gctcctattt gtgaggagat
     1981 tttatgtcga ggagtgattc ctaaaaaact ctttcgtggt aaagaaaatg tgggttatat
     2041 agtcggtgct attatctttg ccttgcttca tttaccgaca aatctacctt ctcttcttat
     2101 ctatggagga atgtcaactg tgctaacttg gacagtttat aaaacccaac gtttggaaat
     2161 gtctatctta cttcacatga ttgttaatgg aatttttttc tgtttgttgg ctctcgtggt
     2221 gattttgagt cggacattag ggatctctgt ttaagatttt tgacaattgc ttacttgttt
     2281 ctgctgggaa aaagatgaat gcaatcgtgt ccatcttttt ctttttatgg taaaatagag
     2341 aaataatatg atgaaaagcc ttgagggagt gaccgatatg tcaactaaag ccaatcatgc
     2401 aaagacagct atttgcggaa ttatcaatgt aaccccagac tccttttcgg atggtggtca
     2461 attttttgct cttgagcagg cactccagca ggctcgtaaa ttgatagcag aaggggctag
     2521 catgctagat atcggcggag aatcgactcg gccgggaagt agctatagct atgttgagat
     2581 agaagaggaa atccagcgtg ttgttccagt tattaaggct attcgcaagg aaagtgatgt
     2641 cctcatctct attgatactt ggaagagcca ggtagcagag gctgctttgg ctgctggtgc
     2701 caatctagtc aacgatatca ctggtctcat gggcgatgaa aaaatggctc gtgtggttgc
     2761 taaggctgga gcgaaagtgg ttatcatgtt taatccagtc atggctcgtc ctcagcatcc
     2821 tagctcgctc atctttcctc attttggctt tggtcaagct tttacagagg aagagttagc
     2881 tgactttgaa acattgccaa tcgaagactt gatggatgct ttctttgaac gagcactagc
     2941 gagagcaaat caagctggaa ttgcacaaga aaatatcctg ttggatctgg gaatcggctt
     3001 tggtctgacc aagaaagaaa atctgcttct tttacgggac ctggataaac tacatcagaa
     3061 gggctatcca atctttcttg gagtgtcgcg caagcggttt gtcatcaata tccttgaaga
     3121 aagtggtttt gaagtcaatc ctgagacaga acttggtttc cgaaatcggg acacggcttc
     3181 ggctcatgta actagtatcg ctgcgagaca gggtgtagaa gtggtgcgcg tgcatgacgt
     3241 agctagtcac aggatggcag ttgaaattgc ctctgccatt cgtctggctg atgaagcgga
     3301 aaatttagat ttaaaacaat ataaataaga tgaaagaaat tgaaaataat cagtggattg
     3361 ctaactaccg gacggatcaa ccgcattttg gcttggaacg aatggtggaa ctgttagcct
     3421 tgcgtggaaa tccccatctc aaactcaagg tcatccatat cggagggact aacggcaagg
     3481 gttcgaccat tgcttttttg aagaatatgc tggaaaagct agggctgaga gttggggtgt
     3541 tcagctcgcc ctatctcatt cattacacag accagattag catcaatggg gaatccattc
     3601 cagaggctag gctagaagcc ctcatggcag actatcagtc tttgctggag ggagaagcgg
     3661 tcgccaattt acagagtaca accgagtttg agattatcac agccctggcc tatgactact
     3721 ttgccgcaga gcaagtagaa gtggctatca tggaagtcgg tatgggtgga cttttagata
     3781 gtaccaatgt ctgtcagccc attttgacag gaattacgac tattggcttg gaccatgtag
     3841 ccctacttgg tgataccttg gaagccatag cagagcagaa agctggtatt atcaaacaag
     3901 gtattccctt ggtgacaggt cgtatcgctc cagaagcttt ggctgtgatt gaccgcattg
     3961 cggaagggaa agatgcgccg agacttgcct acgggacaga ttatcaggtc agccatcaaa
     4021 agagtgtggt tacaggcgaa gtctttgact atacaagtgt tgtcaggcaa ggtcgcttcc
     4081 aaactggcct gcttggcttg caccagatag agaatgctgg gatggccata gctttacttg
     4141 atactttttg tcaagaacat ggtcgagagc tagcaagcaa tgacctgctt ggtcaagcct
     4201 tggaagaaac aagttggcca gggcgtttgg aagttgtgtc gagagacccc ctgatgattt
     4261 tggatggagc ccacaatccc catgcgatca aggccttatt ggcaaccttg caagaacgct
     4321 ttgcggatta tcgtaaggaa atcctcttta cttgcatcaa aaccaaggcc ttagaggata
     4381 tgttggactt gctgggggcc atgccagata ccgagctaac tctaacacat tttgacgata
     4441 gtcgggcgac tgatgaaagc gtgctggaag aggcggctaa gtctagaaat ctcaactacc
     4501 aaggttggca ggattttcta gagcagaaat tgacagataa aaaagaagag aaaaaaacag
     4561 ttaggattgt cacgggttcc ttgtatttct tgagccaagt gagagcctat ctgatggaga
     4621 ggaaaaacga aaatggatac acaaaagatt gaagcagctg taaaaatgat tatcgaggct
     4681 gtcggagagg acgctaaccg tgagggcttg caggaaacgc ctgctcgagt ggcccgtatg
     4741 taccaagaga ttttttcagg tcttggtcaa acagcggagg aacatttgtc aaaatccttt
     4801 gagattattg atgacaacat ggtagtagaa aaggatatct ttttccacac tatgtgtgag
     4861 caccacttct tgccatttta tggtagagcg cacatagcct atattccaga tggtcgtgtg
     4921 gcaggtttgt ctaagctagc ccgtacggtt gaagtttatt cgaaaaaacc acaaattcaa
     4981 gaacgattga atatcgaagt ggccgatgcc ttgatggagt atctaggtgc taaaggagcc
     5041 tttgttgtca ttgaggcgga acatatgtgt atgagcatgc gtggtgtcag aaaaccaggc
     5101 actgcaacct tgacgacagt agctcgtggt ctatttgaaa cagataagga cctccgagat
     5161 caggcttatc gtttaatggg actataatat tcttcgaaaa tcaaattcaa accacgtcag
     5221 cctccatctg caacctcaaa acagtgtttt gagcagcctg cggctagctt cctagtttgc
     5281 actttgattt tcattgagta taaaaagaat ccgctttggg cggatttttc gagaaaggaa
     5341 tcattatgga tcaactgcag attaaggatt tggaaatttt tgcctatcat ggtctttttc
     5401 cgagtgagaa agaattgggg caaaagtttg tcgtttcagc tatcctattc tatgatatga
     5461 ccaaggcggc gacagatttg gatttaacag cctctgtcca ttatggagaa ctgtgtcagc
     5521 agtggacgac ttggtttcag gaaacaactg aggacttgat tgaaacggta gcctacaaac
     5581 tagtagaacg tacctttgag gcttatcctc ttgtccaaga aatgaagctg gaactgaaaa
     5641 aaccttgggc accggtgcat ttgccactag atacttgctc ggtaaccatt catcgccgca
     5701 agcaacgagc ctttatcgcc ctaggaagca atatgggaga taaacaagca aacttgaaac
     5761 aagccattga gaaaatgcga aatcaaggta tccatattct caaagagtcc agtgtcttga
     5821 cgacggagcc ttggggtggt gtggagcaag atagctttgc taatcaagtg gttgaggttg
     5881 aaacctggct accggcaccg gtcttgttag aaagcttgtt agccattgag tcagagatgg
     5941 gacgggtgag agaagtacat tgggggcctc gtttgattga tttggacttg ctctttgtgg
     6001 aggaccagat cctttataca gatgacctta tcttgcccca tccttacata gcggaacgcc
     6061 tttttgtcct tgagtcctta caggaaattg cgcctcattt tatccatccg acattaaagc
     6121 aaccgatccg caacttgtat aatgctttga aacaatagaa aaaactctag ttttcagtag
     6181 tttgtaactg aaggctagag tttttaaaat aggtcatttt cttctgggag gaggatagtt
     6241 tctctaccgt ccatgtctaa aaccagtact cttgggggat aacgagggtc gaaaggatgg
     6301 ttaaagtcaa aatcaatggc tgtagggagg tgttgacttg aaaagtggaa ggtaatcttt
     6361 ccttggttat taagcaattg aaactcgagt tcttcttcca attcaaagac attttttaag
     6421 aaatggtcga taatatacca aaaagagtca atgatgtcat caggcaagct ggtaacaatg
     6481 ccaaaactag cagaccgcat gtgggtattg gtaaaagcca tatctctgtc tcctttcttt
     6541 tcccttatca tacagcaaat aggattaaaa atcaagaaaa ggtgattttt tgaaaaggat
     6601 tttaactaca gggaaaaaga aggaaaaaaa ttcccaaaaa tctaccgaag ttaataggta
     6661 aattcccaaa ttaacttgat tatataactt tcagttactt tgagaagtta ccgaaaaata
     6721 tttttcatat ctattgactt ttaggggtga aatttggtat gatagtaggc ggtattgttt
     6781 accccatttg aaaggccccg gaaccttcca aatacttttc gatgggaagg aacacccatc
     6841 accgtaaaca aaaatcgaac tatatatagg agaaatcatg aacaaaacaa catttatggc
     6901 taaaccaggc caagttgaac gtaaatggta cgtagttgac gcaactgatg taccacttgg
     6961 acgtctttct gcagtagttg ctagcgtact tcgcggaaaa aacaaaccaa catttacacc
     7021 acacactgat acaggtgact tcgtgattgt tatcaatgct gaaaaagtta aattgactgg
     7081 taaaaaagca actgataaaa tctactacac tcactcaaac cacccaggtg gattgaaaca
     7141 aatctctgca ggtgaacttc gttctaaaaa tgcagtacgt ttgattgaga aatcagttaa
     7201 aggtatgctt ccacacaata cacttggacg cgctcaaggt atgaagttga aagtatttgt
     7261 tggagctgag cacactcatg ctgcacaaca accagaagtt cttgacattt caggacttat
     7321 ctaaggaaag gaacaaataa agtatgtcac aagcacaata tgcaggtact ggacgtcgta
     7381 aaaacgctgt tgcacgcgtt cgccttgttc caggaactgg taaaatcact gttaacaaaa
     7441 aagatgttga agagtacatc ccacacgctg accttcgtct tgtcatcaac caaccattcg
     7501 cagttacttc aactgtaggt tcatacgacg ttttcgttaa cgttgtaggt ggtggatacg
     7561 ctggtcaatc aggagctatc cgtcacggta tcgctcgtgc ccttcttcaa gtagacccag
     7621 acttccgcga ttcattgaaa cgcgcaggac ttcttacacg tgactcacgt aaagttgaac
     7681 gtaagaaacc aggtcttaag aaagctcgta aagcatcaca atttagtaaa cgttaattcg
     7741 aaagaattac tatacttata cagagcacct ttcggggtgt tcttttttta tactttctta
     7801 ctaaattggt gcaattgaca cagttgttgc gactttagtc gcttacaaat gtggctgcaa
     7861 cctgacaagg tcagttgcct caaaacgtta atcaatacaa ttatatcaac gtttcaaagc
     7921 actcaagagt ttacctcatg ggtgcttttt ttatgttttt tgaaaaagtt cacctcaaag
     7981 tttaccttat tttcacctta ttgttttaga gactgtaagg caaattcacc gacggccata
     8041 gggactacat tagaagtatt gacataaatc tgtgttgtac ctgtgtcgct atgtattaga
     8101 gcttcagaaa tggcttctaa gctcattcct gcttgtttag cgagcgttgc tccggtatga
     8161 cgtagtttgt gtggcgtagc gtgtgtaagc tctgggtgtc gttttctgat agttttcatc
     8221 ttattattga gatagtcaga atgtaaaggt ttattgatat tgcctttggt atctatgtaa
     8281 gtaaagatga attgttctgg attactgatg ataccaaact ttgccagttc atatttttgt
     8341 tgttttttcc atgaagtcag aagttgaagt aagtcattag aaatagagaa aatgggaaat
     8401 gtttccacaa taaaacgcat attatcagat ggttaatgcg ttttatattt gggtttgagg
     8461 cttataaaat aggataagcg tactgaaaac ttagatatga caaacaaaat aaaattttta
     8521 aaagctacag agaaacatgg tataatgtat ctaaaggagg tagcatatgt ccttagatat
     8581 agataaagaa aagatgacaa tcatgggaat agcttttgaa aaccgatccg tttttaagag
     8641 tgtttggtat gctttaagta caaatatgat tgaaggatgg cgacctacgg ttagtgatgt
     8701 tgaaaaatta cgagatgaag ctcttgctct ggggatgact taatgaagcg caactgttat
     8761 gaatcttatg actatgttga tccggataat ttatataccg atccgggttc ttcagttcta
     8821 agaaataagc aggaaggaaa aaagcacgtg agcttgaata tcgtatgatt gctagtaaga
     8881 gtttgaaatt atttatcaat cctatcttag ttaatagttt aaaagtagtt tgtttcacaa
     8941 ctgcataagc ttagaaaagt tgaaaagtga taactcttta ataaactctt ggtttgttaa
     9001 aagatatttt gtccactaca ttttttcaat tgactattat ttgtcataga tataacagat
     9061 agaacattgc attcagaata atgaaaaacg catcaaaaat ggtgcgttct gtttttatgc
     9121 taccataaaa ggaggaaaac aaattgaaaa tttttaaggg agagttttat cgaatctctg
     9181 tattaacaga caagctagta aggttagaat actctcgaac tggaaatttt gaggatagaa
     9241 cgacacaact tatctataat agagattttg gccaaaattc gttagattat atcgagacat
     9301 caaatgtact agatattatg acggactatt ttcatctgca ctttaataaa ggaaaattta
     9361 acgccgaaaa tttatttata gaattaaaag gaaattttgc cgtatatggt agtcgctggt
     9421 attttggtga atctattgaa acgttaaaag gaacagctcg gactctggat aaggcagatg
     9481 gagcaatctc gttagaagat ggaattatta gccgaaatgg tatagcctta ttggatgatt
     9541 ctcaaggatt tatttgggat gaacaatctg gttatattga gagagaaaat caaattgacc
     9601 tctatttctt tgcctatggg catgattata gaggagcaat cagagacttt taccatttga
     9661 ctggttcaac acccttgttg ccaagatatg ctttagggaa ttggtggagt agatattggc
     9721 cttatacgtc ggatgaatac ttggatttaa tagacagatt tgaaacagag aaaattccat
     9781 tatctatcgg tgtgttagat atggattggc atataactga cattccagct cgttttggaa
     9841 gtggctggac aggatatagt tggaataaaa acttaatacc taatccagaa cagttattgc
     9901 aacaacttca tgatagaaag ttaaaactct ctttaaatgt ccatcctgct gatggtatac
     9961 gggcttatga agaagcttat cctcaagtcg ccaaacgatt agggttaaat gtagaactag
    10021 aagaacctgc catttttgat ttttttaatc cctcttttag ggaagcctac tttaaagatg
    10081 ttcattatga gctagaaaag cagggagtag atttttggtg gattgactgg caacaaggga
    10141 ctcaaggcat attggatcca ctatggcttt taaaccatta tcattatcag gatagttgta
    10201 aaaatgcaga aggtggtttg attttatcaa gatatgcagg tcctggtagc caccgctacc
    10261 ctgttggttt ttcaggggac actattatta gttggaattc cttaagattt caaccttatt
    10321 ttacagcgac agcatctaat atcggttata gttggtggag ccatgatatc ggtgggcaca
    10381 tgctggggga ttatgacgaa gagctacaaa ctagatggct acagtttggt gtttttagtc
    10441 cgattactcg attacatagt tctagaagtc cttttaatag taaagaacct tggttttttt
    10501 cagaaacaac atctaagatt atgaagaaat accttcgttt gagacatcag atgattccct
    10561 atctatatac catgaatgta aagacacatg aggaaggtgc cccattaatc agtccaatct
    10621 attatttcta tccagagaat gatgagagct ataatgttcc aaaccaatac ttttttggaa
    10681 cagaactgat ggtggctccc attgtagaaa agatggattt gacattccaa tctgcaaaag
    10741 tagatgtatg gttccctgaa ggtgaatggt atgacttttt ttcagataaa aaatacacag
    10801 gtggtgtgaa gttaagtgtt tatagggaca tctcgactac gcctgtgttt gcaaaaagtg
    10861 gtgcaatcat tcccttggtt ggttctgaga taggtatggg tgttgattta cctgaagttg
    10921 tagattggta tgtattccca ggaaaacaac attcttttga aatgcttgaa gatcaaaatg
    10981 gtcaaagata taaaacaaga ttatcaatcg attgggaaat gggaatggta gagttagcat
    11041 tacaaggaga ttctagtatc gttccaagca atagaaaaca tagaattcat tttaaaggaa
    11101 cgaatgtgtc tataattgaa ttgccaaata agaatgatac agctaaattt gaatggaaag
    11161 ataataaaag gacatcttta aatgacgaag tttttagact actaaagaca gcttctcttc
    11221 catatgaatt aaaagataga ttgttaaatc aattcatcaa tgcgaaaaac tctcatgact
    11281 taatgaatat cttgcatcat caggataagg aattgagagg gcgtttgttg gaaatgatat
    11341 ttactagcca aaactaattg atataattag aaagtcattt gtttagtgta tatgaagatt
    11401 ctgtatggta ttcgttatca ctggtcacat tgaaaatgtg aaataagcac ctacaacata
    11461 gactttacaa cacttcatgt ttcatatcat ggggtgtttt tctatttaag tcaaagccaa
    11521 tcaaaaaatc tcactattca tcaggttaaa ggaaacctaa tcatagtgag attgtgaatt
    11581 atagtagagt ttgtatcgcc tcttcaattt gttttgggtc tgtttttgaa gaaaagcgtt
    11641 caaagacttg cccatctcga ccgatgagaa acttagcgaa attccattcg actcgttttc
    11701 ctagtgggcc ggatttctgg tcttttaacc agacatagag agggtctgct tccttaccgt
    11761 tgaccttaat cttggcaaaa cgtgggaagg tggtttgaaa atgtaggcta cagaaggcgt
    11821 tgatttcctc tgcgctgccg ggtgcttgtc ccataaactg attgcaaggg aaatccaata
    11881 tttcgaagcc ctgatcttga tagcgttcat agagttcttg aagtccctgg tactggggcg
    11941 ttaaaccaca tccagtagca gtgttgacaa tcaagagaac tttgccacga tagctatcca
    12001 agggagttgc ttggttgttt tggttcaaaa cggaaaaatc ataaagtgaa gtcatgaggg
    12061 cctcttttct ttttctacat tgtaacagtt tttacttttt tcgtcgagtc ggatggaaga
    12121 tattgttgaa gaataagccc tcaatatgat agaaaccgtt tacaaataac tttaaaaatg
    12181 gtatgattta attaaaccgg tttactttgt tgctataaat ctctaatttg agatttagtt
    12241 cctattgatt ttacaatatg tttattggag tgtatacatg caaacaaaaa caaagaagct
    12301 cattgtgagt ttgtcttcac ttgttttatc aggattttta ttaaaccatt atatgacagt
    12361 tggagcggaa gaaacgacta cgaataccat tcagcaaagc cagaaggaag ttcagtatca
    12421 gcaaagggat acaaaaaatt tagttgaaaa tggtgatttt ggtcagacgg aggacggaag
    12481 cagtccgtgg acaggaagca aagctcaggg gtggtcagct tgggtagacc agaagaatag
    12541 ttctgcagat gcctcaactc gagtcattga ggctaaggat ggggctatca ctatctcaag
    12601 ccctgagaaa ttaagggcag cggttcaccg tatggttcct attgaagcta agaaaaagta
    12661 taaactgcgt ttcaagatta aaacagataa taaagtcggg attgccaagg ttcgtatcat
    12721 tgaggaaagt ggtaaggaca agcgattgtg gaattctgca acgacgtcag gaacaaagga
    12781 ctggcagacc attgaagcag actatagccc gactttagat gttgataaaa tcaagctgga
    12841 gttattctat gaaacaggaa ctgggactgt ttcctttaag gatattgagc tggtagaggt
    12901 agcagcccag ctttctgagg attctcaaac agataaacag cttgaggaaa agattgattt
    12961 accaattgga aaaaaacatg ttttttctct tgcggactat acttataagg tagaaaatcc
    13021 tgacgttgct tcagtcaaaa atggaatttt agaacctctt aaggaaggga caaccaatgt
    13081 cattgtcagt aaagatggca aggaagtgaa aaagattcct ttgaagattc tagcctctgt
    13141 taaggatgca tacacagccc gtttggatga ctggaatggc atcatcgctg ggaatcaata
    13201 ctatgattct aaaaatgaac agatggccaa attaaaccag gaattggaag gaaaggtagc
    13261 tgatagccta tctagtattt caagtcaggc ggaccgcacc tatttgtggg aaaaattttc
    13321 aaattataaa atgtctgcaa atctgactgc cacttatcgg aaattggagg agatggccaa
    13381 gcaagtgacc aatccttctt ctcgttatta tcaagatgaa actgtcgttc gaacagtcag
    13441 ggattccatg gaatggatgc ataaacatgt ctacaatcgt gaaaagagca ttgttgggaa
    13501 ctggtgggat tatgaaatcg gtacacctcg tgccatcaac aataccttgt ctctgatgaa
    13561 agaatacttc tctgatgagg aaattaaaaa atatacagat gtgattgaaa aatttgtacc
    13621 agatcccgaa catttccgaa agacgactga taacccattt aaggctctag gtggaaactt
    13681 agttgatatg ggaagagtaa aagtaatagc tggtttactg cgtaaggatg atcaagaaat
    13741 ttcttctacc attcgctcga ttgagcaagt gttcaagttg gtagaccaag gtgaaggttt
    13801 ttatcaagat ggatcctata tcgaccacac caatgttgcc tatacgggtg cttatgggaa
    13861 tgttttgatt gatggcctgt ctcaactgtt gccagtcatt caaaagacca agaatccaat
    13921 cgataaagat aaaatgcaaa ccatgtacca ctggattgat aaatcgtttg ctcctttgct
    13981 ggtgaatgga gagctgatgg atatgagtcg tggacgctcg atcagtcgtg caaatagcga
    14041 ggggcacgtg gccgcagtag aagtactaag agggattcac cgaatagcgg atatgtctga
    14101 aggagaaacc aaacaacgtt tgcagagtct tgtgaagacc attgttcaat cggatagtta
    14161 ttatgatgtc tttaagaatt tgaagactta taaggatatc agtttgatgc aatccttgtt
    14221 aagtgatgca ggagtcgcaa gtgttccaag aacaagttac ctatctgcct ttaacaagat
    14281 ggataaaaca gccatgtaca atgcagagaa agggtttgga tttggcttgt cactcttttc
    14341 cagtcgtacc ttgaattacg aacacatgaa caaggaaaat aaacgtggtt ggtatacgag
    14401 tgatgggatg ttctatcttt acaatggcga tttgagtcac tatagcgatg gctactggcc
    14461 aacagttaat ccatataaga tgcctggtac aacagagacg gatgctaaga gagcggatag
    14521 cgatacaggt aaagttttac cgtctgcttt cgttggaacg agcaaactag atgatgccaa
    14581 tgcgacagca accatggatt tcaccaactg gaatcaaaca ttgactgctc ataagagctg
    14641 gtttatgcta aaggataaga ttgccttttt aggaagcaat atccaaaaca cttcaacaga
    14701 tactgctgca actacaatcg accagagaaa actggaatca agtaatccat ataaagtcta
    14761 tgtcaatgat aaagaagcct cccttacaga acaagaaaag gattatcctg aaacccaaag
    14821 tgtcttttta gaatcgtccg attcgaaaaa gaatattggt tactttttct ttaagaagag
    14881 ttcaatcagc atgagtaagg ctttgcaaaa gggagcctgg aaggatatca atgaaggaca
    14941 gtcagacaag gaagttgaaa atgaatttct tacgattagt caggctcata agcaaaatgg
    15001 agattcttat ggctatatgc tcattcctaa cgtggatcgt gccaccttca atcaaatgat
    15061 aaaagagtta gaaagtagcc tcatcgaaaa taacgaaacc cttcagtctg tttatgatgc
    15121 caaacaagga gtttggggca ttgtgaaata tgatgattct gtctctacta tttccaacca
    15181 attccaagtt ttgaaacgtg gagtctatac cattcgaaaa gaaggggatg aatataagat
    15241 tgcctactat aatcctgaaa cccaggaatc agctccagat caggaagtct ttaaaaagct
    15301 agagcaagca gctcagccac aagtacagaa ttcaaaagaa aaggaaaaat ctgaagagga
    15361 aaagaaccat tcggatcaaa agaatctccc tcagacagga gaaggtcagt caatcttggc
    15421 aagtctaggg ttcttgctac ttggggcgtt ttatctattc cgtagaggaa agaacaacta
    15481 atttgttcat catctagatg aattacatga aactgtggaa aatagaaaaa atccgaagaa
    15541 ggccaaatat cttcggattt ttcttttaaa aaaggataag ttgatactta tcctttttac
    15601 ttcattatgt gactatagcg ttttgaaact agaataggac a
//
LOCUS       DAVMEI010000030        15629 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVMEI010000030 DAVMEI010000000
VERSION     DAVMEI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.030"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            122..3223
                     /locus_tag="VYQ24_000764"
     CDS             122..3223
                     /locus_tag="VYQ24_000764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1967933.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPFYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     EIEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3306..4313
                     /gene="pfkA"
                     /locus_tag="VYQ24_000765"
     CDS             3306..4313
                     /gene="pfkA"
                     /locus_tag="VYQ24_000765"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1967934.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4372..5877
                     /gene="pyk"
                     /locus_tag="VYQ24_000766"
     CDS             4372..5877
                     /gene="pyk"
                     /locus_tag="VYQ24_000766"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1967935.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDEVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6611..6836)
                     /locus_tag="VYQ24_000767"
                     /pseudo
     CDS             complement(6611..6836)
                     /locus_tag="VYQ24_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7684..8556
                     /locus_tag="VYQ24_000768"
     CDS             7684..8556
                     /locus_tag="VYQ24_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1967936.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8702..9046)
                     /locus_tag="VYQ24_000769"
                     /pseudo
     CDS             complement(8702..9046)
                     /locus_tag="VYQ24_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9189..9500
                     /locus_tag="VYQ24_000770"
     CDS             9189..9500
                     /locus_tag="VYQ24_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967937.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9504..10220
                     /locus_tag="VYQ24_000771"
     CDS             9504..10220
                     /locus_tag="VYQ24_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1967938.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10350..11165)
                     /locus_tag="VYQ24_000772"
     CDS             complement(10350..11165)
                     /locus_tag="VYQ24_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1967939.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11162..12067)
                     /locus_tag="VYQ24_000773"
     CDS             complement(11162..12067)
                     /locus_tag="VYQ24_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1967940.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(12338..12421)
                     /locus_tag="VYQ24_000774"
                     /pseudo
     CDS             complement(12338..12421)
                     /locus_tag="VYQ24_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            12465..14594
                     /locus_tag="VYQ24_000775"
     CDS             12465..14594
                     /locus_tag="VYQ24_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1967941.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14581..15030
                     /locus_tag="VYQ24_000776"
     CDS             14581..15030
                     /locus_tag="VYQ24_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1967942.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTQRYRCGLCRGKLLLVNQPED"
     gene            15088..15360
                     /locus_tag="VYQ24_000777"
     CDS             15088..15360
                     /locus_tag="VYQ24_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1967943.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
BASE COUNT         4820 a         2779 c         3372 g         4658 t
ORIGIN      
        1 gatgacgaaa atacaaatga atagaaaata aattagtaag ctgatgaaat ttttctcaag
       61 agaagccatt tataggtgaa aatggtataa tatagtgaga aggatagagg agaagtgtaa
      121 attgatcgca caactagata caaaaacagt ctatagtttt atggaaagcg tcatttcgat
      181 cgaaaagtat gtgagagcag ctaaagaata cggctacact catttggcta tgatggatat
      241 tgacaatctt tatggcgctt tcgactttct agagattaca aaaaaatacg gcattcatcc
      301 tttgctaggg cttgaaatga cagtgtttgt agatgatcag ggagtgaatt tgcgcttttt
      361 agctctatct agtgtgggct atcagcagtt gatgaagctt tcgacagcca agatgcaggg
      421 ggagaaaact tggtcagtcc tgtcccagta cctggaggat atcgcggtca ttgtgcctta
      481 ttttgataga gttgagtcgt tagaactagg ctgtgattac tatatagggg tttatccaga
      541 aacactagca agcgaatttc atcatcctat cttacctttt tatcgggtca acgcttttga
      601 aagcagggat agagaagttc ttcaagtttt aacagcgatt aaagaaaatc taccgctcag
      661 agaagttccc ttgcgttcga gacaagatgt ctttatatca gcaagttctt tagagaaact
      721 attccaagag tgttttccgc aagctttgga caatttagaa aagcttattt caggcatttc
      781 ttacgacttg gatactagtc tgaaactgcc tcgttttaat ccagctagac cagcagtaga
      841 ggagttgaga gagcgtgctg aactggggct tgttcagaag gggttgacta gtaaagaata
      901 tcaagataga ctagaccaag aattgtctgt tattcatgat atgggctttg atgattattt
      961 cttggttgtt tgggatttgt tgcgttttgg acgatcgaat ggctattata tgggaatggg
     1021 aaggggttct gcagtaggca gtttggtttc ttatgcctta gacatcacgg ggattgaccc
     1081 agtagagaaa aatctgattt ttgaacgctt tcttaatcgt gaacgctata ccatgcctga
     1141 tattgatatt gatatcccag atatttatcg tccagatttt atcagatatg ttggtaataa
     1201 atatggtagt aaacatgcgg cacaaatcgt tactttttca acctttggag ccaagcaagc
     1261 tcttcgagat gtcttgaaac gctttggtgt gccagagtat gaattatctg caattactaa
     1321 gaaaatcagt tttcgtgaca atcttaagtc ggcctatgag ggcaatctcc agtttcgtca
     1381 gcaaatcaat agtaagttag aataccaaaa agcttttgag attgcttgca agatagaggg
     1441 ctatccaaga caaacctctg tccatgcggc tggtgttgta attagtgacc aagatttaac
     1501 caactatatt cctctaaagt atggtgatga aattccactg actcagtatg atgctcatgg
     1561 agttgaggct agcggacttt tgaagatgga ctttctggga ctacgaaatt tgacctttgt
     1621 ccagaagatg caagagttgc ttgctgaaat agaaggtatt caccttaaaa ttgaagaaat
     1681 agatttggaa gacaaagaaa cgttagcttt atttgcctct ggaaatacaa aaggtatctt
     1741 tcaatttgag caaccaggtg ccattcgtct gcttaagcgt gtgcaaccag tctgttttga
     1801 agatgtcgtc gcgactactt ctctaaatcg accgggtgct agtgactata tcaataattt
     1861 tgtggcaaga aagcatgggc aggaagaagt gactgttctg gatccagtac tggaggatat
     1921 tttggctcca acctacggca taatgctcta tcaggagcag gttatgcagg ttgcccagcg
     1981 atttgccgga tttagtcttg ggaaagccga tattttgcgt cgggctatgg ggaaaaagga
     2041 tgcctctgcc atgcatgaga tgagggcttc ctttattcaa ggttcattag aagctggtca
     2101 tactgtggaa aaagcagagc aggtctttga tgttatggag aagtttgcag gttatggttt
     2161 taacaggtca cacgcctatg cctactcagc cttggccttc cagttggctt attttaaaac
     2221 acattatcca gccatttttt atcaggtcat gttaaattct tccaacagtg attacttaat
     2281 agatgcactt gaagcaggtt ttgaagtagc ctctctatcc atcaacacca ttccctatca
     2341 cgataaaatt gccaacaagg ccatctatct aggtttgaaa tccattaaag gagtcagtaa
     2401 tgatttagct ctctggatta ttgaaaatag accttattct aacattgaag attttatagc
     2461 taaattacct gagaattatc tgaaacttcc tctgctagaa cctttggtaa aagttggtct
     2521 tttcgattca tttgaaaaaa atcgtcaaaa agtatttaat aatttagcta atctatttga
     2581 atttgtgaaa gagttgggaa gtttgtttgg agatgctatt tatagttggc aggaatcgga
     2641 agattggacg gaacaagaaa aattttatat ggaacaagag cttttaggga taggtgtcag
     2701 caaacatcca ctacaagcta ttgcaagtaa ggctatttac ccgattaccc caatcggaaa
     2761 tttgtcagaa aatagctatg ctattatctt ggttgaagtt cagaaaataa aagtgattcg
     2821 taccaaaaag ggtgaaaata tggccttctt acaggcagat gatagtaaga aaaaattgga
     2881 tgtcactctc ttttcagact tatatcgtca ggttggacag gaaataaaag agggagcctt
     2941 ctactatgta aaaggaaaaa tacaatcacg tgatggccgt ctgcaaatga ttgcacaaga
     3001 aataagagaa gcagttgctg aacgcttttg gatacaggtg aaaaatcatg aatcggatca
     3061 agaaatttca cgcattttag aacaatttaa aggcccaatc ccagtcatca tccggtatga
     3121 agaggaacag aaaaccatcg tttctcccca tcattttgta gctaaatcca atgaattaga
     3181 ggagaaattg aatgaaatcg ttatgaaaac gatttatcgc taaaaatacg gaaaatagaa
     3241 gaattttaaa atcaaatgtg atataatcaa gaagaatgtt aaaagaaaaa ggagcataaa
     3301 ccaatatgaa acgtattgct gttttgacta gtggcggaga cgcacctggt atgaacgctg
     3361 ccatccgtgc agttgttcgt caagcaattt cagaaggaat ggaagtgttt ggtatctatg
     3421 acggatatgc tggtatggtt gccggtgaaa ttcatcccct agatgcagct tcagtagggg
     3481 acatcatttc tcgtggtggt acttttcttc actcagctcg ttatccagag ttcgctcaac
     3541 ttgaagggca acttaaaggg attgagcaat tgaaaaaaca cggaattgaa ggtgtagttg
     3601 ttatcggtgg tgacggatct taccacggcg ctatgcgttt gactgaacat ggcttcccag
     3661 ctattggtct tccaggtaca atcgataacg atatcgttgg tactgacttt acaatcggtt
     3721 ttgacacagc ggttactact gccatggacg ctatcgataa gattcgtgat acatcatcaa
     3781 gtcaccgtcg tacttttgta attgaagtta tgggacgtaa cgctggtgat atcgctcttt
     3841 gggctggtat tgcaactggt gctgatgaaa tcatcatccc tgaagcaggc ttcaagatgg
     3901 aagatatcgt agcaagcatc aaagctggtt atgaatgtgg taaaaaacac aatatcatcg
     3961 tcttagctga aggtgtgatg tcagcggctg aatttggtca aaaacttaaa gaagctggag
     4021 atacaagcga ccttcgtgta acagaacttg gacatattca acgtggtggt tctccaactg
     4081 cgcgtgaccg tgttttggcg tcacgtatgg gtgcacatgc tgttaaactt cttaaagaag
     4141 gtatcggtgg tgttgcggtt ggtattcgta acgaaaaaat ggttgaaaat ccaattcttg
     4201 gtactgcaga agaaggggca ttgtttagcc ttactgcaga aggtaagatt gtggttaaca
     4261 acccgcacaa agccgatatt gagctatcta gcttgaataa gagcttgtca taatttacaa
     4321 tttagttaat aagaccaaaa aggtcatata tataaaggag tcacaaaaat catgaacaaa
     4381 cgtgtaaaga tcgttgcaac tttgggtcct gcggtagaaa tccgtggtgg taaaaaattc
     4441 ggtgaggacg gatactgggg tgaaaaactt gatgttgaag cttcagctaa aaacattgct
     4501 aaattgattg aagctggtgc taacacattc cgattcaact tctcacacgg cgaccaccaa
     4561 gaacaaggtg agcgtatggc aactgttaaa cttgcggaaa aaattgcagg taaaaaagtt
     4621 ggtttccttc ttgatacaaa aggacctgaa atccgtacag aattgttcga aggtgaagct
     4681 aaagaatatt catacaaaac tggtgaaaaa attcgtgttg caactaaaca aggaatcaaa
     4741 tcaactcgtg aagtgattgc gttgaacgtt gctggtgctc ttgatatcta tgatgaagtt
     4801 gaagttggtc gtcaagtttt ggttgacgat ggtaaacttg gtcttcgtgt ggttgctaaa
     4861 gatgatgcaa ctcgtgaatt tgaagttgaa gttgaaaacg atggtatcat cgctaaacaa
     4921 aaaggtgtga acatccctaa cactaaaatt cctttcccag ctcttgctga acgcgataac
     4981 gacgatatcc gtttcggtct tgaacaaggt atcaacttca tcgcaatttc attcgtacgt
     5041 actgcaaaag atgtgaacga agttcgtgca atctgtgaag aaactggaaa cggacatgtt
     5101 caattgttcg ctaaaatcga aaaccaacaa ggtatcgata acttagatga aatcatcgaa
     5161 gcagctgatg gtattatgat tgctcgtggt gatatgggta tcgaagtacc gttcgaaatg
     5221 gttccagttt atcaaaaaat gattatcaag aaagtcaatg ctgcaggtaa agttgttatc
     5281 actgcaacaa acatgcttga aacaatgact gaaaaaccac gtgcaactcg ttcagaagta
     5341 tcagacgtat tcaacgctgt tatcgacgga actgacgcta caatgttgtc aggcgagtct
     5401 gcaaacggta aatacccact cgagtcagta actacaatgg ctacaatcga caagaacgct
     5461 caagctcttc ttaatgaata cggacgtctt gattcagatt catttgagcg taactctaag
     5521 acagaagtaa tggcttctgc tgttaaagat gctactagct caatggatat caaattggtt
     5581 gtaactctta ctaagacagg tcatactgca cgtttgattt ctaaataccg tccaaatgct
     5641 gacatcttag cattgacatt tgacgaattg acagaacgtg gcttgatgtt gaactggggt
     5701 gttatcccaa tgttgacaga tgctccatct tcaactgacg atatgttcga aatcgctgaa
     5761 cgtaaagcgg tagaagcagg tctcgttgag tcaggcgatg atatcgttat cgttgctggt
     5821 gtgccagtag gagaagctgt tcgcacaaac acaatgcgta tccgcacagt acgttaagaa
     5881 aaatataaaa acctatcata tccagctttc tagcctgtat gatgggctct ttttttgtcc
     5941 agggggcaaa aagggggcaa aactttcata atggattgat actcttcgaa aatctcttca
     6001 aaccacgtca gcgtcgcctt accgtatata tgttactgac ttcgtcagtt ctatctacaa
     6061 cctcaaagca gtgctttgag caacctgcgg ctagcttcct agtttgctct ttgattttca
     6121 ttgagtatga aataagatat gcataaattg attagaaagt caaatgaatt tctacaaatg
     6181 ttttagcaat cgcaatctac ttgtctagat tcgatctgat atattttcga tttaatgata
     6241 tggtatttaa aacctccaaa gtagcttgct ccattctttt acctacgtga gtctagatgt
     6301 tatttactgt tttagcgttt ttgtgttcta ctctaaccat tatagcattc ttctcagcta
     6361 gtgtactaag gagtgtgtgc ctgaaaatat gggaactaag gggctggttt atcggtttct
     6421 ctagtttagt atttgccttt tgcaaagtga tcttaaatgc ctttctctaa atttacatat
     6481 cactattgtt taacaaaatc taatctattt taggtcactt attctttttt tgaaatgtag
     6541 aatgaacttt ttcaaagttt ttcgaatctt ttaaaatctg tttgttttat atcgccattc
     6601 tccccctttt ttaattctcc ctatatagcc tgacaacttt tccgatggta cgaatatggt
     6661 tgctttcgtc taggtggatg tcggagtatt cgggattgag tttttttgag gtagccttgg
     6721 cggagtttct tgacatagtt agtgccgtct acttggaaga tgccgatggt attatagtca
     6781 atctgtgggg tattcttgat aaataggtag tcgctgtttc ttatctttgg ctccatggac
     6841 ttgctgacga cataagcgat tgggtcgtag tcgtctggga taatggaaac tccataacta
     6901 aatcgtcgtc ctgcatcaag cggctacctg cagagataaa ctacctaaca cgagagtaag
     6961 tagtctgtct gtagtcgtcc agtctgatga tttttacgat acttcgtttt tctgatcata
     7021 cagttgcctc tcggcatagg tcagaacttt accttgtctg ggtggtgccc gttggtcgta
     7081 gatagattgg atatcgctag gagaatcctt ttgaactgga ggaaagaggg catcaatcaa
     7141 gctactgaat actttaacta agtcaaatat agtatttttc ttagtagacc taaccctttt
     7201 ttcataattt ctaatggtgt ttttacttat acctatctta gtacccaatt cttattgagt
     7261 ccaaccatta ctagtctata ttgttttata gttgattgag tttggaatag tacgctgtag
     7321 ctgctaaaac atttctagga attaatttgc ctttcctaat agagttgttc atatcttatt
     7381 tcaatctact atatttttca cctctaacaa tcgcaatctc ttctttatcc atgaacgaaa
     7441 acgctttcta tctttgtaag taaagcatca cacgaaatcc acgaaaatga aaacctttgt
     7501 tgtgttttcg taaaaaattt gttgacagag cacgaaacgc ctactataat taaatcaagt
     7561 ttaaggaaat aacaaaaaaa caaatcggag ggaaagacga tgaacacatt aaatgagaaa
     7621 gtaatcaata tctgtaaagc agtagttaaa gaaactttaa tccaagacat ttaggagaaa
     7681 gaaatgaaga aatcaagaaa attagccaca ttaggaatct gttcagcctt gtttttaggc
     7741 ttggctgctt gtcaacaaca acatgctact tctgagggga cgaatcaaag gcaaagcagt
     7801 tcagcgaaag ttccatggaa agcttcatac accaacctaa acaaccaggt aagtacagaa
     7861 gaggtcaaat ctctcttatc agctcacttg gatccaaata gtgttgatgc attttttaat
     7921 ctcgttaatg actataatac cattgtcggc tcaactggct tatcaggaga tttcacttcc
     7981 tttactcaca ccgaatacga tgttgagaaa atcagtcatc tctggaatca aaagaagggc
     8041 gatttcgttg ggaccaactg ccgtatcaat agttattgtc ttttgaaaaa ttcagtcacc
     8101 attccaaagc ttgaaaagaa tgaccagttg cttttcctag ataatgatgc gattgataaa
     8161 ggaaaggtct ttgattcaca agataaggaa gagtttgata ttctattttc gagagttcca
     8221 actgaggcaa ctacagatgt caaggttcac gctgaaaaga tggaaacatt cttctcacaa
     8281 tttcaattca atgaaaaagc tcgaatgctg tctgtagtct tgcacgacaa tctggatggc
     8341 gagtatctgt ttgtaggcca cgttggggtc ttagtacctg ctgatgacgg tttcttattt
     8401 gtagagaaat tgactttcga agagccctac caagcgatta aatttgctag taaggaagat
     8461 tgctacaagt atttgggcac caagtatgcg gattatacag gcgagggact ggctaagcct
     8521 tttatcatgg ataatgataa gtgggttaaa ctttaatagt gttatgatat gacagtaatc
     8581 ttaggatttt tatttctatt tttgatgact tggtgggtta tttcaatcgt taatagccag
     8641 aaaccacgca aaaatgaaac atttattggc tatatccaaa gatacgatat tgatgggaat
     8701 ggctatcaat cattaccgtg tcctcagaat tgagaggagt tcttgaaatc gtaaaactac
     8761 tttgaacaag agttacttca acccattggc tccgacggat taagttgctt tcgtgaacac
     8821 caaaatcagc cgcaatttct tcataagtgc ggtattctcg cacatattga agagtggcca
     8881 taagaaggtc ttctaggctt aatttaggtt ttcgtccccc ttttgtgtgt ttaagttgat
     8941 aagcagtttt taatacagct aacatctctt caaaagtggt gcgctgaaca ccaacaagac
     9001 atttaaatcg tgcatcagtt agttgcttac ttgcttcata attcatagaa ctactatacc
     9061 atattttgtt tcgcaggaag tctaatgcta aaatcttatc agtgagaaga tttcttaaag
     9121 tttattcttt gtagtttcat ctgtattagt atataattta acttagagaa aataggagga
     9181 ctaatcgaat ggaattaaaa gattttacag aaaaagaaca ggaaatgatt aagaaaaggc
     9241 ttacaatgtc taatattagt gacaaagaaa ctactgagaa gattctggcg ctagtaccac
     9301 aagacttgat taagcgaatc ccgttttttg tcagaaaaca tgctacaaca cgtacgatta
     9361 aacgcatttc aattgaatac cctgaactct acgctgtagc tcaaacaagt ggtgagattc
     9421 cagaaaaaga acgcgaagaa ttgcgtcaga ttatcacgac tatctttgaa caaaagatga
     9481 ataagcatag tattaagtag agaatgaaaa aatattttat tagcggtttg ggaagcaatg
     9541 cctatcatag caaggatttt tttcaagaac tagattcgca ggtctatttt ctaaatccat
     9601 atgaaaagca tcttcgagat gaaacagaat tgaaatcatg gtttaaaaat gagattgtag
     9661 aggaagaatc tatctgtctg ataggccatt ctcttggagg agatttagct cgttatttcg
     9721 catcggaatt tgaagaagta aagaaactga ttcttttgga tggtggctat ctagatttag
     9781 ataagatttt acctttggat acagagttag aggaaactaa aaattatatc aaatctcaaa
     9841 ttgtttcgga cttagctctt cttatttcta aagagaagtc cgaagcaaag cattggtcag
     9901 aaaatatgga gaaagctgta agacagtcct atcactggaa tgttgagtat aatagatatg
     9961 agttggctat aaattatgaa aatatagaag cgatactccg cctacggaga aaaatacaag
    10021 cttttaagag agaagtggga gataccttgt ttattagtcc tcgctatcct aatggagcta
    10081 catggagaga ggaagcccta aaagaattgc cagactattt taatactatt tttttagaaa
    10141 actttggcca tgagctttat actcaagcac ctaaagaaat cgctagtcta atgaatgagt
    10201 ggctcgctta ttttctatga gctacttgta ttgctggaaa tggtataaga tgaaaatcta
    10261 caagtcacta attttcatat aacaaaaaaa cgaacaggct ctttttctgt tattcagaag
    10321 agaacttgtt cgtttttatc ttgattttct taaaaagctc tatatacata gggattttct
    10381 ggtttatcga ctttggtaaa gctgtcgatt tccaaggttg gaaggttttg tttgagttct
    10441 ttatggtagt gattgaccag ttttcctttg gctgtaatcc aggtgttgtt ttcatactgc
    10501 cgggtattgc ccttggtcag caatccatag acaccagaat ctgcgataca gtggataatg
    10561 ccgaatcgga acagaaattg actattggca tgactggggt cgttatagac aaagcctgta
    10621 aactggattg tcttgccctc aaattcatct ggatagtcgt agatagcctc catgacttcc
    10681 atatagtttt cattagtgat ctgaatacta tcttgggata agtatttatc cgccgccgtt
    10741 cgcatttcct tttcataggc tgattttgaa aaataagaac tagtatctgg tttcaaatat
    10801 tggcttgtcg tcccttcact tgtctgaata gctagatccg ttccttccga tagggggaaa
    10861 tgataacctt tagcagaaac ggtctgagaa tccaagctaa cagttgggaa agttaagccg
    10921 atgacaagtg gaatagccag aagagaaata ctcgtcatct tggccaatcg gctgttcaga
    10981 tgactgtggg ttttgacttg cttcatccag atatacaatt gaacaattgc caagataaag
    11041 gaaagcacca tggagatata ggccagatag gaatagtgca tgttgatgta ctggtttagt
    11101 ttgcccgaca gatggaggta aatagtcagt tcaaaatagc cagctaaaac taaaaatcga
    11161 atcataggat gactccaatc aagagagaat aggctaaaac gaccagagtc acaatcgtaa
    11221 tgaagtgact aataaatcgt gctttcaagt aatttttcat catgagaata tttttgatat
    11281 ccagcattgg gccaatgacg agaaaggcca gaactggtgc caaaccgaaa ctcgagagaa
    11341 gagaagcacc tataaaggca tccgcctcac tacagagcga aagaagaaag gccaaaatca
    11401 tcaagagcag gatggcaaaa agaggagtcg cactgataga ggtcagaatc cgagtcggaa
    11461 cgtagacctg tattatagaa gcaaagaggc agccaaatac caaataacgc cccgtatcaa
    11521 aaaattcatc aatggcctgc acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag
    11581 aaaaatcatg ctcatgacaa gccagacgat tttctttctg aatcggttct tgccagaaaa
    11641 atcctagaaa aattcctagt attacagcca caagaatgga acccagagct cgtaataggg
    11701 cgacatggaa ggagttgcca aaggcagaat aggtcgcaaa aagaacaatg ggattgataa
    11761 caggtgctgt cacaagaaaa ggaacggctg tgtaactggg aacctttttt tccagaaaac
    11821 gattgatgat ggggacgatt ccacattcac aagaagggaa aagcataccg acaaaggtcc
    11881 caaaaaagat tctcccccaa cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga
    11941 tataaacttc aatcagccct gagacaatgc ttcctattag aacgaaggga agggcttcaa
    12001 tgataatgga gagaaaaatg gctccagttt gaagtacact agatgggagt tgttgaaaag
    12061 cagtcatcta gtttttcttt tctgctttgg cttttttctc tttgtcatca gggaaagtaa
    12121 cttgtttcaa atcagggagt ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt
    12181 ttgtaaattt tacagccata ggtgtacctc gattctaaag tctacctcta ttatactatt
    12241 attttgcgga aaagggagtt ttttaatttg ttttcgatag ttgatacact tcgaaaatct
    12301 cttcaaacca cgtcagcttc gccttaccgt atatatgtga cttatccaca acctcaaaac
    12361 agtattttga gcagcctgcg gctagcttcc tagtttgctt tttgattttc attgagtatg
    12421 aaaaggattg gagaaagcct tccagcttgt ggtataatag atttatggat aaaaaatatg
    12481 aaaaaatctc tcaggatttg ggagtgatgt taaagcaaat tgataccgtt ctaagtttga
    12541 cagctgaagg ggcgactatt ccctttatcg cgcgttatcg caaggacatg actggtagtc
    12601 tggatgaggt ggcgattaag gctattattg atttggataa aagtctgaca aatctcaatg
    12661 accgtaagga agctgtctta gctaagattc aagaacaagg taagttgacc aaggaattgg
    12721 aagaagctat cttagttgcc gaaaaattag cagacgttga agaactctat cttccttata
    12781 aggaaaagcg tcgtaccaag gcaaccattg cccgtgaagc tggactcttt cctcttgctc
    12841 gtttgatttt gcagaatata gttgacttag agaaagaagc tgaaaagttc gtctgtgaag
    12901 gatttgcgac tggcaaggaa gccttgaccg gtgcagttga tattttggtc gaagccttat
    12961 cggaagatgt gaccttgcgt tctatgactt atcaggaagt gctgagacac tctaaactta
    13021 cttctcaagc caaggatgaa agtcttgatg aaaagcaggt ttttcagatt tattatgatt
    13081 tttcagagac agttggaact atgcaaggct atcgtacctt ggctctcaat cgtggggaga
    13141 aacttggtgt cttgaagatc ggttttgaac atgcgacgga ccgtattctt gccttctttg
    13201 ctactcgttt caaggtgaaa aatgcttata ttgatgaagt tgttcagcaa tccgttaaga
    13261 aaaaggtctt gcctgctatt gagcgtcgta ttcggacaga attaactgag aaagctgaag
    13321 agggagctat ccaacttttt tctgacaatc tgcgcaatct cctcttggtt gctccattga
    13381 aagggcgcgt ggttcttggg tttgacccag cctttcgtac aggtgccaag ctagctgtcg
    13441 tggatgcaac aggaaaaatg ctgacaactc aggtcatcta tcctgttaaa ccagcatcag
    13501 ctcgtcaaat cgaagaagcc aagaaagatt tagcagattt aattggtcaa tacggtgtag
    13561 agattattgc cattggaaat ggaacggcca gtcgtgaaag tgaagctttt gtagcggaag
    13621 ttctgaaaga tttccctgaa gtcagctatg ttatcgttaa tgaaagtggt gcttctgtct
    13681 attctgccag cgaacttgct cgtcaggagt ttccagactt gaccgttgaa aaacgctctg
    13741 ccatttctat cgcccgtcgt ttgcaagatc ctcttgcgga attggtcaaa atcgatccta
    13801 agtcaattgg tgtcggtcaa taccaacacg atgtcagtca gaagaaacta tctgagagtc
    13861 tggactttgt tgtcgataca gtggttaacc aagttggtgt caatgtcaat acagctagcc
    13921 cagctcttct ttcacacgta gctggactca ataaaaccat ttctgaaaat atcgtcaaat
    13981 accgtgagga agagggaaaa atcacttcac gctcccaaat caagaaagtt cctcgtttgg
    14041 gggccaaggc ttttgaacag gctgctggtt tcctccgtat ccctgaaagt agcaatatcc
    14101 ttgataatac aggagttcac ccagaaaatt acgctgccgt taaggaactc ttcaaatgct
    14161 tggacattaa ggatctgaat gaagaggccc aaagtaaact caagtcgctt tcagtcaagg
    14221 aaatggcgca agagttagac cttggtccag aaactcttaa agatatcatt gcagatcttc
    14281 tcaaaccagg tcgagatttc cgtgattcct ttgatgctcc agttctccgt caagatgtcc
    14341 tagatatcaa ggacttagtg gttggacaaa agctagaagg agtggtgcgt aatgttgttg
    14401 atttcggtgc ttttgttgat atcggaattc atgaggacgg tctcatccat atttcccata
    14461 tgagtcgcaa gtttatcaaa caccctagtc aagtggtggc agttggagat ttggtgagcg
    14521 tttgggttaa gaaaattgat actgaacgtg aaaaagttaa tctgtctctc ctagctccaa
    14581 atgaaactga ctgattacgt caagcaggtt tcactagaag acttcggcag accttttatc
    14641 catcatgtcc agtggaatag gcgtctacgt tcgacaggtg ggcgattttt ccccaaagat
    14701 gggcatttgg attttaatcc caaggtttat caggaactgg ggttggacgt ttttaggaaa
    14761 attgtccgac atgaactctg tcattatcac ctctattttc aagggaaagg ctatcaacac
    14821 aaggatcggg attttaagga actgttgaaa gcagtggatg gattacggtt tgtaccatcc
    14881 ttgcccaata gtaactccaa gccaatcaag ctctatcgtt gccaatcctg ccagcaaact
    14941 tatcagcgta agcgtaggat tgatacccaa cgctatcgct gtggactttg tcgaggaaag
    15001 ttgctattag taaatcagcc tgaggactga tgaaagccga gcctatccgt gatatactag
    15061 tctaacctag cagaaagagg aaatatcatg aacagtaagt tttataaaat gagacgaaat
    15121 cgcatgattt caggagtttt agcaggtctg tcagacaagt ggaactttga tgttaccctt
    15181 gtacgttttc tgtttgccat ttttacagtc gcaaactttg gacttggggt gattatctat
    15241 atcatcctag cttctatcat gccaaccaag gaagaaatcg aagcagaaat gtacggaaca
    15301 ggcccacgta aacgcaaaga agctcaagcc attgacgata atgaaggctg gttttggtga
    15361 ggttttacaa aagatttgga atacgcaaaa agctttaaat caatagtcta aactgcttga
    15421 tttaaagctt ttttactttt atactcaata aaaatcaaag tgcaaactag gaagccagcc
    15481 gcaggctgta atttagtcgg caatgtgaag ttgatatagt ttgcactttg atttttgaat
    15541 agtattaata atatttcgcc agcttcatcc acaaactata gtggattgga actagaatag
    15601 gacacctata cttctaaaaa attgttaga
//
LOCUS       DAVMEI010000031        15010 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVMEI010000031 DAVMEI010000000
VERSION     DAVMEI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.031"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(106..1302)
                     /gene="tuf"
                     /locus_tag="VYQ24_000778"
     CDS             complement(106..1302)
                     /gene="tuf"
                     /locus_tag="VYQ24_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1967944.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2213..3082)
                     /locus_tag="VYQ24_000779"
     CDS             complement(2213..3082)
                     /locus_tag="VYQ24_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1967945.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3305..3826)
                     /locus_tag="VYQ24_000780"
     CDS             complement(3305..3826)
                     /locus_tag="VYQ24_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1967946.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            complement(3784..4863)
                     /locus_tag="VYQ24_000781"
     CDS             complement(3784..4863)
                     /locus_tag="VYQ24_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1967947.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEELAE
                     LISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNVTDKKLSSMT
                     TTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKEGRQEKL
                     AESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAYQKTVQQ
                     LTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(4974..5228)
                     /locus_tag="VYQ24_000782"
     CDS             complement(4974..5228)
                     /locus_tag="VYQ24_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967948.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5586..6119)
                     /locus_tag="VYQ24_000783"
     CDS             complement(5586..6119)
                     /locus_tag="VYQ24_000783"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1967949.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPVLYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVMDLL
                     REVPTIFECLECFFKTR"
     gene            complement(6106..6321)
                     /locus_tag="VYQ24_000784"
     CDS             complement(6106..6321)
                     /locus_tag="VYQ24_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1967950.1"
                     /translation="MGKERREQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(6288..6431)
                     /locus_tag="VYQ24_000785"
     CDS             complement(6288..6431)
                     /locus_tag="VYQ24_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV1967951.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            6665..8383
                     /locus_tag="VYQ24_000786"
     CDS             6665..8383
                     /locus_tag="VYQ24_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1967952.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8494..8841
                     /locus_tag="VYQ24_000787"
     CDS             8494..8841
                     /locus_tag="VYQ24_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1967953.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9120..9956)
                     /locus_tag="VYQ24_000788"
     CDS             complement(9120..9956)
                     /locus_tag="VYQ24_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1967954.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(9969..10598)
                     /locus_tag="VYQ24_000789"
     CDS             complement(9969..10598)
                     /locus_tag="VYQ24_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1967955.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10608..11249)
                     /locus_tag="VYQ24_000790"
     CDS             complement(10608..11249)
                     /locus_tag="VYQ24_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1967956.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11395..12624)
                     /locus_tag="VYQ24_000791"
     CDS             complement(11395..12624)
                     /locus_tag="VYQ24_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1967957.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELYDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12614..13780)
                     /locus_tag="VYQ24_000792"
     CDS             complement(12614..13780)
                     /locus_tag="VYQ24_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1967958.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            13924..14937
                     /locus_tag="VYQ24_000793"
     CDS             13924..14937
                     /locus_tag="VYQ24_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1967959.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT         4519 a         3209 c         2691 g         4591 t
ORIGIN      
        1 tgattttcat tgagtattag ttcaatatct aaaaaaagaa ccttgccaag caagattctt
       61 ttagtgtctg acttaaataa ttgttcttct gggaactaaa tcgaattaag cttcgatttc
      121 tgtaaccata cctgaaccaa cagtacgtcc accctcacgg atagagaatg tagtaccttg
      181 ttctacggcg attgggtgaa tcaactcaac gtcgattgtc acgttatcac caggcattac
      241 catttcagta cctgctggaa gttcgattga acctgtaacg tcagtagtac ggaagtagaa
      301 ttgtggacgg tagttgttga agaatggagt gtgacgtcca ccttcttctt tagtaaggat
      361 gtagacttca cctttgaatt tagtgtgtgg gttgattgaa cctggtttag cgataacttg
      421 tccacgttcg atttcatcac gttgaacacc acgaagaagg acacctacgt tatctccagc
      481 aagaccttcg tcaagttgtt tacggaacat ttcaacacca gtaacaactg ctttttgagt
      541 ttcttctttg ataccaacga tttcgatttc gtcgttgact ttaacgatac cacggtcgat
      601 acgtcctgaa gcaactgtac cacgtccagt gattgagaat acgtcctcga ctggaagaag
      661 caatggtttg tcagtgtcac gttctggttc tgggatatac tcatcaactg tgttcatcaa
      721 ttccataacg atgtcttcgt atttagagtc accttcaaga gctttaagtg ctgaaccttg
      781 gataactgga agatcgtcac ctgggaagtc gtattctgac aataggtcac ggatttccat
      841 ttcaaccaat tcaagcaatt cttcgtcgtc aaccaagtca actttgttca tgaagacgat
      901 aaggtgttta acaccaacct gacgtgaaag aaggatgtgc tcacgagttt gtggcattgg
      961 tccgtcagtt gaagctacta caaggatagc tccgtccatt tgagcagcac cagtgatcat
     1021 gtttttaacg tagtccgcgt gtcctggagc gtcgatgtga gcgtagtgac gtttttcagt
     1081 ttcgtactca acgtgcgcag tgttgatagt gataccgcgt tcgcgttctt ctggagcagc
     1141 atcgatagac gcatagtctt taggttggtt aactgatgaa ggcaagcgac gtgccaaaac
     1201 agttgtgata gctgcagtta gggtagtttt accgtggtca acgtgtccga tagtaccaat
     1261 gttaacgtgt ggtttactac gatcgtattt ttcttttgcc atttgagtaa aagcctccaa
     1321 taaaatatat tttatagata gacagtaggc aatacagtct aactttcctt actattttat
     1381 caaatttcag ctaaattgca agtgttttac aacgtttttg tgaattattc ttggtaaaaa
     1441 tttaactaac taatagaaag tactaaatag acttatcctt caaggagttt tcagcattat
     1501 ccatccactc acttgctgaa aatgtaaaca ttctctggtt tatatagtaa aatgaaacaa
     1561 gaatagggca aatcgatcag gacaatcaaa tcgatttcta acaatgtttt agaagttgag
     1621 gtgtactatt ctagtttcaa tctactatac aacctcaaaa ctgtgttttg aacaacctgc
     1681 ggctagcttc ttagtttgcc ctttgatttt cattgagtat aaaattgcag ataaaacgat
     1741 aacaatcata ggactcagca gagtcaatgt aggtcgaaga atagcccccc taatcgctta
     1801 ttttatagca aaatgaaata gaaactgaac attttgttga ggaaggtaaa tcaattcctc
     1861 acaatgtttg agaaagaagg gaggactatt ccaacttcaa cccactataa agcagactta
     1921 caagataggg aacccatctc cgataatata tagtagaatg aaaactgcac aatttgattg
     1981 gagaattcaa accaatttct aacaatgttt tagaaacaag ggtctattat ttcaactgca
     2041 acctactata gcccattgaa gctggaatag cccactgaga cttctaaacc actgctggag
     2101 aggagtttgt ttagagtttc tttcatttcc ctaaaaaatc aaaaagagag aaccagtctg
     2161 atcctctctt acttatagat ggtgctcact caaatgttaa aggagctatt tcttagagat
     2221 aaaggaattt gaatacagct actgccgcaa ttgctgctgc gataggtgct actactggta
     2281 cccaagaata ccaccatttt gaatcgcctt tatgctcacc aagaactgat tttggaagga
     2341 aagcatgaag gagacgtggt cccaagtcac gggctgggtt caaggcaggt cctgtaggtc
     2401 ctccaagtga tgttaccaaa gccataacga ggaatccaag tgccaagtga gccacagaaa
     2461 gtcctgaagc agtgtgtgga gccacctgtg ctttaatagc caagtcagaa aaatcaactg
     2521 tttgtcctgc ttctattgcc ttttgtttca tgaattgaag cacttcagca ccaaagaagt
     2581 ttttagtcaa accaagagct gcaaagaaca aaacaaatga accaacaaac tcattaatca
     2641 aaccattgac agttgctgca tagcgacttt cttttgtacc atggtcaata cttgaaatag
     2701 ttgagaaagt tcccaagatg ttatttggtt tttcagtttt caagtagaat ggacggtatg
     2761 ttgccacaac taaggcttgg ccaaagatag cccccaagac ttgcgcgata atgtaaggta
     2821 ccacttgtgc ccaagggaaa agaccactaa ctgcaagccc tagagtgaaa gcagggttga
     2881 tgtgattccc agatacgtta ccaaacatca aggctgggat cataaccccc ataccataac
     2941 caacagcgat gacgatccag ccactttggt gacctttcgt acctttaagt tcaacgttgg
     3001 caactgcacc attcccaaga atgatcaaaa tggcagttcc caaaaattca gtggcatact
     3061 tcagtgccca tgtgaaatcc atttgctaga ttctccttaa aattttttag atattcctta
     3121 ttttatcaat ttttacccta tttagcaact gatttctgga aatattttta aaaaaatagg
     3181 attcatagtt tcccagttca acgtacttgt aagcgttttt attcttcacg aaagacctca
     3241 ttctcaaaac caggaaaaag cccacatctg tgatcttttc cttgttccta gattctaaaa
     3301 tctattattt ttcatcttct tttttcttga agaatcctaa tcctagacca gctaacaagg
     3361 tcatgatacc agcactagcc aaggtagcat tagcttctgt acctgtgttt ggcaattcct
     3421 ctctcttacc tgtctcataa gtcggaactt ctgggtctgg attcactgga gtttcagttt
     3481 ttggagtacc tggttctgga gttggtttat ctggtgttga taaacggtca taccttaccg
     3541 ttatttcttt atcactagag tctgacgtaa cttcttgtga ttcaactgtt ggaatatctg
     3601 gatctttgta cttgtcaatc ttaccagata taacctcgtc ccagtttcct gttgtccatt
     3661 caccgtaggt tacaactccc gtgaccttgt tctcagtttt tgtacggctt aaggttacag
     3721 gttgaacaac atcttctttt acattttggt tcgtaacttt atcaacgtaa tgaatgatac
     3781 gcgttatagt cttcgtctca gtagaggttg ctgttttggg aaccactgtt tcctcaacat
     3841 tctcacggta gtaatagtca actgttgcac cgtcttctgg tacgcatttg caggagttgc
     3901 taccaaggtg tatgttgttt tccttgtgat aactcggtct tctttgtcct cagttgttgt
     3961 tttcccttca atagtttttg attctgtggt atactcagaa cctatcgcta aatcagcttt
     4021 tataacagac tctgccaact tctcttgcct accttcttta tagtaattgg ctgttactgt
     4081 agcagtggtt ggcgcttcgc tttctctata aactaaggtc actgttctac cttcgcttac
     4141 aatattccca gttaaacttg cagaatttgt atctgcttct ttaaaagtat aatattttcc
     4201 gtcagtagta gtcatgctac tgagtttttt atctgtgaca ttatagctgg taccaatcag
     4261 ttgtttttta ttggtaatgt aggttccgtc actttctttt tctccaattc cagtatcatt
     4321 tttcaatgat agcaaacgcc cttgttcatc aacatagcga actttcacat tttctgagat
     4381 tagttctgcc aattcttcgg ttattttccc tttctcttct tcgggaatat ttagttttgg
     4441 aatgattttt tcaacaacgg ttcgtgatgg ttccacagta tcttggatga ctgaaaagtc
     4501 agctagaatt gggagattat actgaacacg gtgactttga gtgtttactc ctactctttc
     4561 attattctct gaaaatactt gtacggtata agaaacaaca tcttttccta atagaacatc
     4621 cccagtagag aaatagccgc cttttcctag tttgctatct ccagagccca cttctttcct
     4681 aatcttatca gatagttttt taccagtcag tacattcgtt cgcacaatcc ctttgtctac
     4741 ccctacaaag tgggagaact ttttgaactc ttcagaacca gatctagccc aaccattatt
     4801 aagggcattt gcttttgtat ttgtattctc tctcaaaggt ttggcgatta gaattatatt
     4861 catcggcact tagagttgct gctatatctg actcttgaat accaacttcc ttactaccat
     4921 ttctagcggc agtatatgtg tatttatctg tttctacttc tagctgatta tccttaacat
     4981 gataggtaat tttctcctgg tataagagtc ttcagtagtt tatatactct gtcatcatct
     5041 ttagctttag aagcagctga gtttagccaa gttggggtat taggagtatc tttatcaacc
     5101 tgtttcaaga cattattcaa gtcatttgct agcctattgg ttatcctcac tgtgaaagtt
     5161 ttcttccagt ctgaaccact tttattagac ttagatatgc tcatgaaatc ctggtcaaca
     5221 ttcaacacaa tatcgccttg ttttttagta tcttccgttg cttctgcttc aaattgatca
     5281 tggagttttt gttgctctgg tgtatagttt ttttatttac acgatttaat gtactatttt
     5341 ttattttagt attaagaact ccattaatac gatttttaaa ctctgcttca aaaaatccct
     5401 taatttgttg gtgcgcaagt tcaacattca aacctttctt atacaagtct tcattctgat
     5461 acaagtcttc attctgaacg atttcggcta cacggtgaat ggtttgatcg tataccgtct
     5521 tctcttaacc agcagtatcc ttagttttaa tgactactcg ataggttcct ttagaaccgt
     5581 tatagtcatc tagttttaaa aaagcattcc aaacattcaa aaatagttgg aacttccctt
     5641 aataaatcca tcactttctt tttcaagata gcccaagctt gctcaatagg attcaaatcc
     5701 ggtgaataag gtggtagagg taagaaaaag tgtttatctt ggatgcagag ttcatccaaa
     5761 atgttcttgg gatgaaaact agcattgccc atgacaataa catgaggtgt cttcaaagca
     5821 ggtaggagtt gcgttttgaa ccactccaca aagaaatcgc ttgtcatgct tttcttgtaa
     5881 atcatgggag ctataaactc tccgtctact tgtcctgcaa caattgaagt tcgctcaaaa
     5941 cgacgtccgc taatcttttc atagactttc tcccctctag gagcccctgc ataaggacga
     6001 tagaggtagc ggtcgattcc tgtttcatca atatatagga ctggtaaatc ttttaggtta
     6061 tccaaaatat caagaaactc agcgactttc tctggctctt gttccttaaa gctcgtcatc
     6121 tttttttaaa gtgaccttaa tctgctttaa agctgcccat actgaaggaa cagcacaatc
     6181 aaaatgtgcc gcaatttccc gtaaaaaagc atctggatga gcctctacaa aggctttcaa
     6241 ttcctctaaa ggaatctttc ggtttttgac gactcgcttt ttcctctcta tgtgtccttg
     6301 ttcacgtctt tcttttccca cgtgaagaga gttctgacgc caacaccaaa aaactttgct
     6361 gcctcgacat ggctgtgccc ctctttgatg taatctaatg ctcgctgttt aaaatcagta
     6421 ctatatgcca tagctttatt ataatatgct tgtttaaaac taagcaacta tatcaatgtt
     6481 ttgttatgag aagacttact ttttttactg aaattgaatt tttcctagcc tcaacatcat
     6541 ataaccccaa aattcaatct gacctggttt gaagagatgt tcgaagcaga taaggtttta
     6601 ctaaaactaa aaaaaatgat ataattaaac tgattaaatg ttttaaaaat aaaggagaat
     6661 ttgaatgtct taccaagaaa attaccagaa atgggttgat tttgtggagc ttcctgacta
     6721 ccttcgtcaa gatttggaaa atatggacga aaaaactaag gaagatgcct tctatacaaa
     6781 tcttgaattt ggtactgcag gtatgcgtgg cttggttggt gctggtacaa accgtatcaa
     6841 catctacgtt gttcgccaag ctactgaagg attggctcgt ttgattgagt caaaaggtgg
     6901 aaacgagaaa gaacgcggtg tagcaattgc ctacgatagc cgtcacttct cacctgagtt
     6961 tgcctttgaa tctgcggcag ttcttgctaa acacggcatc aaatcttacg tatttgaaag
     7021 ccttcgtccg actccagaac tatcatttgc agttcgtcat ctcaactgtt tcgcaggtat
     7081 catggtcaca gccagccaca accctgctcc atttaacggt tacaaggttt acggtgaaga
     7141 cggtggacaa atgcctccac acgatgcgga cgctttgact acttatatcc gtgcaatcga
     7201 aaacccattt gcagttgaag ttgctgatgt ggaaactgaa aaagcttctg gcttgattga
     7261 agttatcggc gaagctgttg acatagaata ccttaaagag gttaaggaca taaacatcaa
     7321 cccagccttg attgaagaat ttggtaaaga catgaagatt gtctacacac cacttcatgg
     7381 tactggtgaa atgttggctc gtcgtgctct tgcccaagca ggatttgact ctgttcaagt
     7441 tgttgaagcg caagcaactg ctgacccaga cttttcaact gtaacatctc caaacccaga
     7501 aagccaagca gcctttgccc ttgctgaaga acttggtcgt caagttggtg cagatgttct
     7561 tgtcgcaact gacccagacg ctgaccgtgt tggtgttgaa gttcttcaaa aagatggtag
     7621 ctacctcaac ctttcaggta accaaatcgg tgctatcatg gctaaataca tcttggaagc
     7681 tcacaaaaac gctggaactc ttcctgaaaa tgccgctctc tgcaaatcta tcgtttcaac
     7741 tgacttggta acgaagattg ctgaaagcta cggcgcaact atgttcaacg tcttgacagg
     7801 tttcaaattt atcgctgaaa aaatccaaga attcgaagaa aaacacaacc acacttacat
     7861 gatgggattt gaagaaagct tcggttactt gattaaacca tttgtacgtg ataaagatgc
     7921 catccaagcc gttcttgtag ttgctgaact tgctgcctac taccgttctc gtggtttgac
     7981 acttgctgac ggtatcgaag aaatctataa agaatatggc tactacgcag aaaaaacaat
     8041 ctctgttact ctttcaggtg tcgatggtgc tgaacaaatc aaagcgatta tggctaaatt
     8101 ccgcaacaat gctccaacag aatggaacgc aacagctatc actgtcgtag aagacttcaa
     8161 ggcacaaact gctactgttg ctgacggtac tgttacaaac ttgacaactc ctccaagtga
     8221 tgtgttgaaa tacacacttg ctgacggttc atggattgcc gttcgccctt caggtacaga
     8281 accaaaaatc aagttctaca ttgcagttgt aggggaaacc aacgaagaat cacaagctaa
     8341 gattgctaac atcgaagcag aaatcaatgc atttgtaaaa taaagaccta taaaagatga
     8401 gaccaaccaa cctcatcttt tttcgtatca aatctaagca attttagaaa ctttatggca
     8461 tactagactt atcaatgaaa gcgaggtaat ctcatggaac aatttttaga taacatcaaa
     8521 gaccttgaag tcactacagt tgtgcgtgcg caagaagctc ttgataaaaa agaaactgca
     8581 accttcttta tcggtcgcaa aacttgccct tactgccgta aatttgcagg tacattgtca
     8641 ggtgtcgtag ctgaaaccaa agctcacatt tacttcatca atagtgaaga accaagccaa
     8701 ctcaatgatt tgcaagcatt ccgctcacgc tatggaatcc caactgtacc aggttttgtt
     8761 catattacag atggacaaat caacgtccgt tgcgactctt caatgtcagc acaagaaatc
     8821 aaagatttcg caggattgta aaaaatcagc taacaagtct taaaatcaca aaaacgcata
     8881 atgtaaggtg caaaaacctt gatattatgc gttttattgt tcctaaaaca gttacatcag
     8941 tatttcatta cagaacattt taattttgga agtcaaatca atttttaaca atgttttaga
     9001 aatcacagtg ggctattcca tattcaatta tccatattca atcgctatta tcgttttcct
     9061 agattacaaa aaaaactacg ttttgctaat caaaacgcag tttcagccac tattttatct
     9121 tactgtcctt cttttacttc tttggttgct acatcttctc caaaccattt ttggctgatt
     9181 tcttggaact tgccgtcctt gtaaagacta gaaaaagctt catttatctt cttaaccaag
     9241 tttgtatctt ccttacgggc tccaaccgca aaagcttctg tttctagtcc aactgtaaag
     9301 acattataat cgtttaaaac accttctgct tctaaataat agtttgcata gacacggtca
     9361 atcaatagac catcaattcg atcgtttttc aaatcaatca aggcttcatt aaaggtttgg
     9421 tattgattcg cttccttatt agcgacaata ttcttcaaaa gttctggatt tgcttcaaag
     9481 tccgcataac cagatgaacc agcttgagct cctaatgtct ttccagtcat atcctttgca
     9541 gtcgtgatac cagatgattt cttcgtaacc aatacctgct cattcttcat atatgagtta
     9601 ctgaaagcca ccttttcacg gcgttcgtct gtagcggaat agccattcca aatcagatca
     9661 atcgttcctt ttgtcaattc agcttctttc aaatcccaat caatcggttg ccaatttacc
     9721 gtgattccgt atttttcaaa aacagctgta gctaaatcaa tatcaaatcc tgcataagaa
     9781 ccatctttct gagcaaatcc cattggaaca aaagtactat caaatccaat agtaatagac
     9841 ttgttagact ggtactttga ccaattatct ccactagttt cgctagaatt tttcccacaa
     9901 gctactaaga acaaagcagt catcagactg actaatacaa gcatccattt tttcatccat
     9961 ttttcctcct atttaggttc tactttcaat aatacatctg cgatattttc agcaaactgc
    10021 aaatcatggg taaccacaat ctgggtcatc ccaagttccc tattttgcaa gattagcttc
    10081 tccacttcca aacgtaattc tggatccagg gcagaagttg gttcatcgta gccaatgatt
    10141 tctgggtcaa tcatcatagc acgcgccaaa gccactcgct gcttttgccc accagatagt
    10201 gagaaaggat aggcctctgc gtgtcctcct agtcctaact gttccaagag tccactcgcc
    10261 ttcttctcag cctcttcctg cttcattccc atggtcttca caggcgataa agtcaaattt
    10321 tccagaactg atagatgagg aaatagttga aaatcttgga agacaaatcc cagtaggttg
    10381 cgcttctgca attcatccag ctctaaaggt tgtccattat aaaagatttg ccctgaatca
    10441 atggtttcaa gacctgcaag catacgtaaa agagttgtct tacctccacc agaaggtcca
    10501 acgatagcca ggatttgctt ttcaggaata cttagactga aattagacag gatttgtttg
    10561 tctccaaaga ctttattgat atttcgtaat tctaacatgg cagcctccta tctataataa
    10621 ctatacttct tctcaacttt tttggaaata attgtcacaa tcccaatcaa aatcaaataa
    10681 atggctcctg ccaagaacat aggaactaga ctagcatcgc ggttagcagc tgttcgacta
    10741 gccaagataa ggtctgaaat tccgagagca tagaccaaag aagtatcctt gaccaaactc
    10801 ataacttcat taaagacact aggaagaaca atcttggtca cttggggcaa gataatatag
    10861 cgcactctgt caaaagggct aaactttaag accttggcag cctcatactg tcctcttgga
    10921 atagtgtcaa ttcccccacg gaaaatttct gcaaaataag ctgcatagtt gagaacaaag
    10981 gcaataatag ctgcaggaag gcggtctaaa cgaatcccaa tacttgggag cacataatag
    11041 ataaaaatca gttgcaagag taagggggtt cctcgcataa cccagatgta aatgttaatc
    11101 agataatgga ggggcttcca atggacttgc aaggcaaagg caatcaaaac gcccaaggga
    11161 atcgaaaata gcaagaccag tgcaaagacc tgtacagtcg tgctcgcacc attcagtaaa
    11221 ctcggtaata tctcaaataa ataagacata ctgcacctcc taaaagtaat tgttcctatt
    11281 atagcatgaa taaacaagat taccaagtat ttttgtaaaa agattctacc atatacaaat
    11341 aaaaaccgta gctatcaaaa gagataaact acagttctat atgtttaaac attcttacaa
    11401 tctctcaaac agttcttgca tttgcacatc atctggaacc agttttaagt aagtgtgagc
    11461 atggactttt gcttcttcaa aatgtcccaa ttcacgcaag agatagatat agtgttccag
    11521 aaattctgga ttgtccttca aatctcctgt caactcttga taatactcat aagcagtatc
    11581 caaatcgtcc atttcttgat aagaacgagc aatcatccac ttggtcaaaa gattttctgg
    11641 ctcctcactc tgcaattcta gaatatcctc ataacgctcc tgctccagat aaatagtggc
    11701 taaacgaagc aagatttctt ctgtatcctc agcgtcttct tttgcagtaa ggagataatt
    11761 ttctgcacca ctagcatcat acaattcata agaaaattgt gaagcagcta gcaagaggcg
    11821 agtttcaaag ggatttttct ctaatccttg cttagcgata cgcagggctt cttgaacttg
    11881 atgttcctta tgtaaagcct gactgtaccc atactcatag ccttcaaagt caggagaaat
    11941 ggtatcaagc tgcttaaagt agagggtggc tttttgatat tcttcttgat caaaataaag
    12001 actggccaac tcaaaagctg ttaagtcatc gtattctaac tccagggctt tttctaaaaa
    12061 ctcagtagcc gtttcaaatt tccctaactg agcataggca aagccaattc gttgataggt
    12121 ggaaatgccc gtttgctcat aaatcgagcg attatctaac tgggcatagg cttgaatagc
    12181 cgcttggtaa ttttccaact cactatccaa ctctgccaaa cccaatatca agagagaatc
    12241 ctctgagtag gtcaaggcct ccaataattt ctcacgtgcc acatctgtca aaccttccag
    12301 ctggtaaagg tctgccttca gagccaaaga cgagacatac cagtcactgt cagcttggat
    12361 ttcctcaaga taggtaaagg cttcttctat ttgaccatcc tcgctagcaa ttgcagctag
    12421 attaagatga acctctggaa aatcctctac aattttcagg taaatttcct tggcctgagg
    12481 atagaaacca atcccttcaa gataagttgc caattcatac agaagatcac ttgaatcatt
    12541 ttctaaagct ttggcgaaat aatgctcagc cttagttaaa tcttgctcct ccaaagcctg
    12601 taacatctgt tgactattgt tcactcttga cttcctcacc ctctaattca tatagaccac
    12661 tgactacctt ataccattcg aaaatggctg aaatgacaac cttagcagag gcataaaccg
    12721 gaataccaag taaaactccc cagataccaa acatagatcc tgaagttaac aaaacaaaga
    12781 gaacattaat agggtggatg tttaattgac ttcccaaaat caatggagag acaaaacggc
    12841 cttcaatagt ttgttctacg ataaagacaa tcactacttt caaaagcatg actggaccag
    12901 caatcaaacc caatactaga gcaggaagca tggctagaaa gctaccaaga taagggacca
    12961 gatttaaaat accagcagta acccccagcg taaccgcata gcgtagacca ataatcttga
    13021 agaagatgat aaacattact gctacaataa tagccactgt cacttgccct cgaacatagt
    13081 tggacaactg ttgattcaca tctgataaaa cttgtccaac aggttccttc aattttcttg
    13141 gaatgaattg ggtcaaatag ttacgcaagc ctttcccatc acgcaagaga taaaagagca
    13201 tgaaaggaac gataatcaag gcaacaatca cttgagaagc cccgctaata aaggcactca
    13261 cccagttgac tgcctgagat gaaaccttac ttgccaaaac tgtagcctgg ctagaaaaat
    13321 tggtcaaaac ttgctctaat tgaggtctga aatcatctgg caggtgctgg gctaccaatc
    13381 cattaacaat cctatctata tcttctaagt aaacaggaac gtttcttgca aaggtcaaaa
    13441 cctgacgttg cagatttgga atggcgactg ccaagcccca aatgataaag agagcgatga
    13501 taacaaagac aatagtgata gctataacac gattaacctt atgcttctcc atccaatcaa
    13561 caataggatt caacaaataa tataacaaac cagacaaaat gactggcaac atcacaactg
    13621 ctaaaaagtc taaaacaggt gaaaatagaa aactaatctt acttaaaata aaaagattca
    13681 gtcccaataa taaggttact aaaaataccg taattgcctt gttatctaaa aaccacttga
    13741 aaaaccaaga taggctaaaa tgtttctctt tttgctccat aaatacttcc tttctattgt
    13801 tctctcatta taccattttt gaaacaaaat aactcttttt taaagtgctt acatctaatt
    13861 ggatatcttt aatttacttg tttttgtgat ataatagtct tatctttaca cagaggtata
    13921 gtaatgaaag aaactgttta ttttggaact tatacacgtc gtacttctca agggatttac
    13981 aaggcagact ttgatacaga aactggtcag ctttcaaatc tagaactttt tgcagctgag
    14041 ccaagtccaa cctaccttgc ctttgaccag caccaacatt tatacactgt tggtagccaa
    14101 gacgataagg ggggaattgc agcctatcaa actgacggga ctgtgttaaa tcatgttgtt
    14161 gaagaaggag ctccccactg ttatgttgct gtcgatgaaa agcgtgattt ggtttacgca
    14221 gccaactatc acaagggaca agtccttgtt tataaacgcc aggaagatgg tagtcttcta
    14281 cttagtgata tggatcaaca cagtggccaa ggtccacatg aaaatcaagc ttccccccat
    14341 gttcactata cagatttaac acctgaccac tatctagtga cctgcgactt gggaactgac
    14401 caagtcatca cctatgacct cgatcaagaa ggaaaattat ctaagctcta tacctatcac
    14461 agcaagccag gagcaggctc acgccatatc attttccata accactataa aatcgcttat
    14521 ctcatttgtg aactcaatag tactatcgaa gttttaatct acgatggcgt tggcgaattt
    14581 gaacgtatgc aggttatttc aactttacca gaagcttacg aaggctttaa tggaaccgct
    14641 gctattcatc tctctaaaga cggtaaatac ctctacgctt ctaaccgtgg ccatgattct
    14701 atcgcagtat ataccatcct tgcggacggt agcttagagt tattagaaat cgttccaacg
    14761 catggtcaga ctccacgtga ttttgatttg acacccgacc aaaaatttct cattgttgtc
    14821 catcaagact ctgacaatgc aactgtcttt aaacgtaatt gtgacaatgg tcgtctagca
    14881 gaactctcca acgacttcca tgttcccgaa gcagtctgca tccgttttgc tccttaaaac
    14941 accataaaaa atgaacttgg tattttcaag ttcatttttt cggctctata atatttgtag
    15001 tgggtaaatc
//
LOCUS       DAVMEI010000032        14854 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVMEI010000032 DAVMEI010000000
VERSION     DAVMEI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.032"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..860
                     /locus_tag="VYQ24_000794"
     CDS             <1..860
                     /locus_tag="VYQ24_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1967960.1"
                     /translation="SIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVAVTL
                     ITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIMGRA
                     NLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDNPDM
                     ARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLIIGEV
                     IFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTFKQT
                     ILKGAKLSK"
     gene            857..1615
                     /locus_tag="VYQ24_000795"
     CDS             857..1615
                     /locus_tag="VYQ24_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1967961.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            1705..3465
                     /gene="dnaG"
                     /locus_tag="VYQ24_000796"
     CDS             1705..3465
                     /gene="dnaG"
                     /locus_tag="VYQ24_000796"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1967962.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRAGI
                     ESAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            3468..4577
                     /gene="rpoD"
                     /locus_tag="VYQ24_000797"
     CDS             3468..4577
                     /gene="rpoD"
                     /locus_tag="VYQ24_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1967963.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            4591..4920
                     /locus_tag="VYQ24_000798"
     CDS             4591..4920
                     /locus_tag="VYQ24_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1967964.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            5035..6078
                     /gene="cpoA"
                     /locus_tag="VYQ24_000799"
     CDS             5035..6078
                     /gene="cpoA"
                     /locus_tag="VYQ24_000799"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1967965.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            6094..7419
                     /locus_tag="VYQ24_000800"
     CDS             6094..7419
                     /locus_tag="VYQ24_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1967966.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            7450..7626
                     /locus_tag="VYQ24_000801"
     CDS             7450..7626
                     /locus_tag="VYQ24_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1967967.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            7685..7819
                     /locus_tag="VYQ24_000802"
     CDS             7685..7819
                     /locus_tag="VYQ24_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1967968.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            7883..9193
                     /gene="obgE"
                     /locus_tag="VYQ24_000803"
     CDS             7883..9193
                     /gene="obgE"
                     /locus_tag="VYQ24_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1967969.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            9203..9361
                     /locus_tag="VYQ24_000804"
     CDS             9203..9361
                     /locus_tag="VYQ24_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967970.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            10448..11707
                     /locus_tag="VYQ24_000805"
     CDS             10448..11707
                     /locus_tag="VYQ24_000805"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1967971.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            11723..12274
                     /locus_tag="VYQ24_000806"
     CDS             11723..12274
                     /locus_tag="VYQ24_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1967972.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            12267..13544
                     /locus_tag="VYQ24_000807"
     CDS             12267..13544
                     /locus_tag="VYQ24_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1967973.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFWVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            13560..14420
                     /locus_tag="VYQ24_000808"
     CDS             13560..14420
                     /locus_tag="VYQ24_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1967974.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            14645..14755
                     /locus_tag="VYQ24_000809"
                     /pseudo
     CDS             14645..14755
                     /locus_tag="VYQ24_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         4462 a         2555 c         3443 g         4394 t
ORIGIN      
        1 tttccattat ttctcaagga tttgtctggg ctattctagg tctgggaatc tttatgacat
       61 ttaggatttt aaactttcca gatatgacga cagaaggttc cttccctctt gggggagctg
      121 ttgctgtcac tttgataacc aaaggcgtga acccattttt agcgacactt gttgctgtag
      181 gagcaggttg tttggctgga atggcagcag gccttcttta tacaaaaggg aagatcccaa
      241 ccttgctctc agggattttg gtgatgactt cttgtcactc aatcatgctc ttgattatgg
      301 gacgtgcgaa tttaggcctg cttggaacca agcaaattca ggatgttttg ccttttgatt
      361 cggatttgaa tcaactcttg acaggtctca tctttgtgag tattgttatt gctctcatgc
      421 tctttttctt ggacactaaa ctcggacaag cctatattgc tacaggggat aatcctgata
      481 tggctagaag tttcgggatt catactggac gcatggagct catgggcttg gtcttatcaa
      541 atggtgtgat tgcccttgca ggtgccctca ttgctcagca agaaggttat gccgatgtgt
      601 ctcgagggat cggggttatc gttgtggggc ttgcaagttt gattattgga gaagttattt
      661 tcaagagttt gagcttggca gagcgtttgg ttactatcgt tgtaggttct atcgcttatc
      721 aatttttagt gtgggcagtt atcgcacttg gctttaatac aagttacctt cgtttataca
      781 gtgccttgat tttagcagtc tgcctcatga ttccaacatt taagcaaaca atcttgaaag
      841 gagccaagtt aagcaaatga cagcaattgt agaattaaaa aatgcaacca aaatcgttaa
      901 aaatggcttt gatgaagaaa agattatttt aaatgatgtt tccttagaaa tttttgaacg
      961 ggactttatc acgattttgg gcggaaatgg tgctggaaaa tcaaccctct ttaacactat
     1021 agcagggacc ttatcactaa ctagtggaac tatccgtatt ttaggtgaag aactcactaa
     1081 gttttcaccc gagaagcgtg ccaagtacct gtctcgtgtc ttccaagatc caaagatggg
     1141 gacagctccc cgtatgacgg tcgctgaaaa tcttttaatc gccaagtttc gtggtgaaaa
     1201 gcgtggattg ttaccacgac gcttgactag ctataaggat gaatttcagg caaccattga
     1261 aaaagtagga aatggtcttg agaaacactt gaatacaccg attgagttct tatcaggtgg
     1321 acaaagacag gctttgagtc tcttgatggc aaccttgaag cgacctgaat tactcctgtt
     1381 agatgagcat actgctgccc tggatccaaa gactagtgtt gctttgatgg aattgacaga
     1441 tgaatttgtt aagaaagatc agctaacagc ccttatgatt actcatcata tggaagatgc
     1501 tctcaaatac ggcaatcgct tgattgtcat gaaagaagga cggattatcc aagatttaaa
     1561 ccaagaagaa aaagcaaaaa tgaaaatctc tgattattat caactctttg aataaagtag
     1621 ggaaaatgag tgaaatggtt tacttttttt ctgaaataaa gtatactata taaagtaaac
     1681 tatgataaca tggaggtatt gtgtatggtt gacaaacaag tcattgaaga aatcaaaaac
     1741 aatgccaaca ttgtggaagt cataggagat gtgatttctt tacaaaaggc aggacggaac
     1801 tatctagggc tctgtccttt tcatggtgaa aaaacacctt ctttcaacgt tgtagaggat
     1861 aagcagtttt atcactgttt cggttgtggt cgctcaggtg atgtctttaa gttcatcgag
     1921 gagtaccaag gggttccctt tatagaggca gtccaaatct taggtcagcg tgttggaatt
     1981 gaggttgaaa aaccgcttta tagtgagcag aagccagcct cacctcacca agctctttat
     2041 gatatgcacg aagatgcagc caaattttac catgcgattc tcatgacaac gactatgggc
     2101 gaagaggcca gaaattacct ttatcagcgg ggtttgacag atgaagtgct taaacatttt
     2161 tggattggtt tagcacctcc agaacgaaac tatctctatc aacgtttgtc tgatcagtat
     2221 cgtgaagagg atttactgga ttcaggcctg ttttatctct cggatgccaa tcaatttgta
     2281 gacacctttc acaatcgcat tatgtttccc ctgacaaatg accaaggaaa ggtcattgcc
     2341 ttctcaggtc gtatctggca aaaaacggat tcacaaactt ctaagtataa aaacagccga
     2401 tcgactgcaa tttttaacaa aagttacgaa ttatatcata tggatagggc aaaaaaatct
     2461 tctggaaaag ctagtgagat ttacttgatg gaaggattca tggatgttat tgcagcctat
     2521 cgggctggaa tcgaaagtgc tgtggcgtcg atgggaacgg ccttgagtcg agagcatgtt
     2581 gagcatctga aaaggttaac caagaaattg gttcttgttt acgatggaga taaggctggg
     2641 caagccgcga cattgaaagc attggatgaa attggtgata tgcctgtgca aatcgtcagc
     2701 atgcctgata acttggatcc tgatgagtat ctacaaaaaa atggtccaga agacttggcc
     2761 tatctattaa cgaaaactcg tattagtccg attgagttct acattcatca gtacaaacct
     2821 gaaaacagtg aaaatctgca ggctcagatt gagtttcttg aaaaaatagc tcccttgatt
     2881 gttcaagaaa agtccatcgc tgctcaaaac agctatattc atattttagc tgacagtctg
     2941 gcgtcctttg attataccca gattgagcag attgttaatg agagtcgtca ggtgcaaagg
     3001 cagaatcgca tggaaggaat ttccagaccg acgccaatca ccatgccaat caccaagcag
     3061 ttatcggcta ttatgagggc agaagcccat ctactctatc ggatgatgga atcccctctt
     3121 gttttgaacg attaccgttt gcgagaagac tttgcatttg ctacacctga atttcaggtc
     3181 ttatatgact tgcttggcca gtatggaaat cttcctccag aagttttagc agagcagaca
     3241 gaggaagttg aaagagcttg gtaccaagtt ttagctcagg atttgcctgc tgaaatatcg
     3301 ccgcaggaac ttagtgaagt agagatgact cgaaacaagg ctctcttgaa tcaggacaat
     3361 atgagaatca aaaagaaggt gcaggaagct agccatgtag gagatacaga tacagcccta
     3421 gaagaattgg aacgtttaat ttcccaaaag agaagaatgg agtaataatg gcaacaaaac
     3481 aaaaagaagt aacaacattt gacgtacagg tagcagaatt tatccgtaat cataagcaaa
     3541 aagggacagc aacagatgat gaaatcaatg caagtctggt tattcctttt accttggacg
     3601 ctgatgggat tgaagatctc ttgcaacgga ttcaggatgc aggaatttct atcacagata
     3661 acgaaggaaa tccaagtgcg cgtgttctta gcaatgaaga agaaccagaa ctcagcgatg
     3721 aggacttgat tgggtcaact tctgctaagg tcaatgaccc tgtccgtatg tacttgaaag
     3781 aaataggggt cgttcctctc ttgaccaatg aagaggagaa agagttggca ctggctgttg
     3841 aagctggtga tatcgaagcc aaacaacgtc ttgcggaagc caatcttcgt ttggttgttt
     3901 ccattgccaa acgctatgtc ggtcgtggca tgcagttcct tgacttgatt caagaaggaa
     3961 atatgggctt gatgaaggcg gttgacaagt ttgactattc taaagggttc aagttttcaa
     4021 cttatgcaac ttggtggatt cgtcaggcta tcactcgtgc tattgcggac caagctcgta
     4081 ccatccgtat cccagttcac atggttgaaa ctatcaataa attggttcgt gaacagcgga
     4141 atctccttca agaattgggg caagatccga caccagaaca gattgctgaa cgaatggata
     4201 tgacacctga taaggttcgt gaaatcttga agattgccca agaaccagta tctcttgaaa
     4261 ctcctatcgg tgaagaggac gatagccacc ttggagactt tatcgaagat gaagtgattg
     4321 aaaatccagt ggattatacg actcgtatcg tcttgcgtga gcaattggat gaaatcttag
     4381 atactcttac agaccgtgaa gaaaatgttc tgcgtctacg ttttggacta gatgatggaa
     4441 aaatgcgcac acttgaagat gtggggaaag tctttaacgt aactcgtgag cgtatccgtc
     4501 agattgaagc aaaggctttg agaaaactac gccaaccaag tcgtagcaaa ccgcttcgtg
     4561 attttattga agactaagag tgaggaaaat atggcttata cagaagagca aattgaaaac
     4621 atcaaaacac ggattttaac agccttggaa gaagtcatcg accctgagtt gggaatcgat
     4681 attgtcaatc ttggtttgat ctatgagatt cgttttgacg gtgacacagg gcaaacagag
     4741 attgacatga ctttgacaac tatgggttgt cccttagcag accttttgac agaccagatt
     4801 tatgatgcta tgatagaggt tccagaagta acggatactg aggttaaatt agtctggtat
     4861 ccagcctgga cagttgaaaa aatgagtcgc tatgcccgca ttgccctagg cattaagtaa
     4921 acatataaaa gcatgtgaga gactgttgga aaagcgagga aatttcccct cttttcctct
     4981 agtctctcct ttcttttgct gattttattc aaagaaaatg atataatagt agttatggag
     5041 aaaaagaaat tacgcatcaa tatgttgagt tcaagtgaga aagtagcagg acagggagtt
     5101 tcaggtgctt accgtgaatt agttcgtctt cttcaccgtg ctgccaagga ccaattgatt
     5161 gttacagaaa atcttccaat cgaggcagat gtgactcact ttcatacgat tgattttccc
     5221 tattatttat caaccttcca aaagaaacgc tcagggagaa agattggcta tgtgcatttc
     5281 ttgccagcta cacttgaggg aagtttgaaa attccatttt tcttaaaggg aattgtgaaa
     5341 cgctatgtat tttcttttta caaccggatg gagcacttgg ttgtggtcaa tcctatgttt
     5401 attgaggatt tggtagcagc tggtattcca cgtgaaaaag tgacctatat tcctaacttt
     5461 gtcaacaagg aaaaatggca tcctctacca caagaagagg tagtcagact gcgcacagat
     5521 cttggtctta gtgacaatca gtttatcgta gtaggtgctg ggcaagttca gaaacgtaaa
     5581 gggattgatg actttatccg tctggctgag gaattgcctc agattacctt tatctgggct
     5641 ggtggcttct cttttggtgg tatgacagat ggttatgaac actataagaa aattatggaa
     5701 aatcccccta aaaatttgat ttttccaggc attgtatcgc cagagcggat gcgcgaattg
     5761 tatgctctag cggatctttt cttgttgcct agttacaatg agctctttcc tatgactatt
     5821 ttagaagctg cgagttgtga ggctcctatt atgttgcgtg atttagatct ctataaggta
     5881 attttggagg gaaattatcg ggcgacagcg ggtagagaag agatgaaaga ggctattttg
     5941 gaatatcaag caaatcctgc tgccttaaaa gatctcaaag aaaaggctaa gaatatttcc
     6001 agagagtatt ctgaagagca tctgttacaa atctggttgg acttttatga gaaacaagcc
     6061 gctttaggga gaaagtaaaa agtgaggtaa tctatgcgaa ttggtttatt tacagatacc
     6121 tattttcctc aggtttctgg tgttgcgacc agtattcgaa ccttgaaaac agaacttgaa
     6181 aagcagggac atgctgtttt tatctttacg acgacagata aggatgtcaa tcgctacgaa
     6241 gattggcaaa ttatccgcat tccaagtgtt cctttctttg cttttaagga tcgtcgcttt
     6301 gcctaccgag gttttagcaa ggcacttgaa attgctaaac agtatcagct agatattatc
     6361 catactcaga cagaattttc tcttggcctg ttggggattt ggattgcgcg tgaattgaaa
     6421 attccagtca tccataccta tcacacccag tatgaagact atgtccatta tattgctaag
     6481 gggatgttga tccggccgag tatggtcaag tatctggtta gaggtttcct gcatgatgtg
     6541 gatggggtta tttgtcctag tgagattgtc cgtgacttgc tatctgatta taaggtcaag
     6601 gttgaaaaac gggtcattcc tactgggatt gaattagcca agtttgagcg tccggaaatc
     6661 aagcaggaaa atttgaaaga actgcgtagt aaactaggga ttcaagatgg tgaaaagacg
     6721 ttgcttagtc tttcgagaat ctcctatgaa aaaaatattc aagcagtttt agcagccttt
     6781 gctgatgttc tgaaagagga agacaaggtt aaactggtag tagctgggga tggcccttat
     6841 ctgaatgacc tcaaagagca agctcagaac ctagagattc aagactcagt catctttaca
     6901 gggatgattg ctcctagtga gacggctctt tactataaag cggcggattt cttcatttcg
     6961 gcatcgacaa gcgaaacgca aggtttgacc tacttggaaa gcttagccag tggaacacct
     7021 gtcattgctc acggaaatcc ttatttgaac aacctcatca gtgataaaat gtttggaacc
     7081 ttgtactatg gagaacatga tttggctggt gctattttgg aagccctgat tgcaacacca
     7141 gacatgaacg agcatacctt atcagagaaa ttgtatgaga tttcagctga gaactttggg
     7201 aaacgagtgc atgagtttta tctggatgcc attatttcaa ataacttcca gaaagatttg
     7261 gctaaagatg atacggtcag tcagcgtatc tttaagacag ttttgtatct tcagcaacag
     7321 gtggttgctg tacctgtaaa aggatctaga cgcatgttga aggcttcaaa aacacagttg
     7381 atcagtatga gagactattg gaaagaccat gaagaataga aagaggaaca gctatgaaaa
     7441 aaacaattaa tgagaagcgg tcgtgataaa aagattgcgg gtgtttgtgc tggggtggcc
     7501 cattatctgg atatggatcc gactatcgtt caagtcattt ggggtgttct tacttgctgt
     7561 tacggagctg gaattgtagc ttacattatt ttatggatta tcgcaccagt agcaactgac
     7621 tattgaaaac tagctcaatt catgctaaga taatagaaaa tagaatgtaa cgaaggagag
     7681 aaaaatggca tttggagata atggaaatcg taaaaaaact atgtttgaga aaataacctt
     7741 gtttatcgtg attatcatgc tagtagcaag tttattggga atttttgcaa ctgcaattgg
     7801 tgccctcagt aatctataaa atagattcaa gaaaatttag tgactgggat ttcccagccc
     7861 ttttttaaag tgagaagaaa taatgagtat gtttttagat acagctaaga ttaaggtcaa
     7921 ggctggtaat ggtggcgatg gtatggttgc ctttcgtcgt gaaaaatatg tccctaatgg
     7981 aggcccttgg ggtggtgatg gtggtcgtgg aggcaatgtg gtcttcgttg tagacgaagg
     8041 actacgtacc ttgatggatt tccgctacaa tcgtcatttc aaggctgatt ctggtgaaaa
     8101 agggatgacc aaagggatgc atggtcgtgg tgctgaggac cttagagttc gagtaccaca
     8161 aggtacgact gttcgtgatg cggagactgg caaggtttta acagatttga ttgaacatgg
     8221 gcaagaattt atcgttgccc acggtggtcg tggtggacgt ggaaatattc gtttcgcgac
     8281 accaaaaaat cctgcaccgg aaatctctga aaatggagaa ccaggtcagg aacgtgagtt
     8341 acaattggaa ctaaaaatct tggcagatgt cggtttagta ggattcccat ctgtagggaa
     8401 gtcaacactt ttaagtgtta ttacctcagc taagcctaaa attggtgcct accactttac
     8461 cactattgta ccaaatttag gtatggttcg cacccaatca ggtgaatcct ttgcagtagc
     8521 cgacttgcca ggtttgattg aaggggctag tcaaggtgtt ggtttgggaa ctcagttcct
     8581 ccgtcacatc gagcgtacac gtgttatcct tcacatcatt gatatgtcag ctagcgaagg
     8641 ccgtgatcca tatgaggatt acctagctat caataaagag ctggagtctt acaatcttcg
     8701 cctcatggag cgtccacaga ttattgtagc taataagatg gacatgcctg agagtcagga
     8761 aaatcttgaa gaatttaaga aaaaattgtc tgaaaattat gatgaatttg aagagttacc
     8821 agctatcttc ccaatttctg gattgaccaa gcaaggtctg gcaacacttt tagatgctac
     8881 agctgaattg ttagacaaga caccagaatt tttgctctac gacgagtccg atatggaaga
     8941 agaagcttac tatggatttg acgaagaaga aaaagccttt gaaattagtc gtgatgacga
     9001 tgcgacatgg gtactttctg gtgaaaaact catgaaactc tttaatatga ccaactttga
     9061 tcgtgatgaa tctgtcatga aatttgcccg tcagcttcgt ggtatggggg ttgatgaagc
     9121 ccttcgtgcg cgtggagcta aagatgggga tttggtccgc attggtaaat ttgagtttga
     9181 atttgtagac taggagactg gtatgggaga taaaccgata tctttccgag atgcggatgg
     9241 taattttgtt tccgccgcag acgtttggaa tgaaaagaaa ttggaagaac tatttaatcg
     9301 tctcaatcca aatcgtgcct tgagattggc acgaactaaa aaggaaaatc catctcagta
     9361 aagaagctaa aaaatcccgt gcctcatcag acacgggatt ttgtggtacg acaggcatgt
     9421 atagcaaact gaatctggaa tagcacagca tatcttctaa aatattgcta taaattgatt
     9481 tgaatttcaa aattaaattg ttttattctt atttcaattt gttatagtat atctgatgtc
     9541 aaagttctcg gcgagtcaaa tagtgattcc caagcctgac tatcgtgagg tagcggatta
     9601 aaatggtctg gggatagacc gttttaagtc tgatgctgga aataagaatt gtcagaagaa
     9661 gggatagcga aatcgtggct ctacgaacag gaacgtgata ataaggcgta tatagcgaat
     9721 aagagggcct caaactctaa agtccaaaaa ggtagtcgta acctatatgc gtaaatcatg
     9781 agagtaattg aattcgtact aagattttct atttttactg taacctttta acgtcctcat
     9841 atcttgtata cacgaggaaa gatgtacgac ttatcccgtg aggtctatca ctataaagag
     9901 aaaacgacag atagaagtga tcctgagtca cggttatctt taaaatagat tgtctgatag
     9961 gatgctagcc tgaaaacgtc ttgttcatga aatataggac ggtatgtata aaacgcttct
    10021 gtgaactgag agaaggggga gaagttcttg ctaaaattta gttgaacagc cgtattccga
    10081 tacttagata agagatctag tcttagctcc tactcagttt caggggataa aaaaggggca
    10141 atagcgattc gagaaagatt atactcttcg aaaatctctt caaatcacgt caatatcgcc
    10201 ttgccgtatg tgtaggatac taactacgtc agttccatct acaatctcaa aacagtgttt
    10261 tgagcaacct gcggctagtt tcctagtttg atctttgatt tttattgagt attagtaatt
    10321 cagttactaa ctcgtcaact ctgatttatc caataaaatt gaaaaggatg gaaaaaagga
    10381 taaatttatg atatacttta ttttgaagac cttattagaa atcttgaaag agtattgaaa
    10441 acttagaatg agaaaaattg ttatcaatgg tggattacca ctgcaaggtg aaattactat
    10501 tagtggtgct aaaaatagtg ttgtggcctt aattccagct attatattgg ctgatgatgt
    10561 ggtgactttg gattgtgttc cagatatttc ggatgtagcc agtcttgtcg aaatcatgga
    10621 attaatggga gctactgtta agcgttatga cgatgtcttg gagattgatc caagaggtgt
    10681 tcaaaatatt ccaatgcctt atggtaaaat taacagtctt cgtgcatctt actattttta
    10741 tgggagcctc ttaggccgtt ttggtgaagc gacagttggt ctaccgggag gatgtgatct
    10801 tggtcctcgt ccgattgact tacaccttaa ggcgtttgaa gctatgggtg ccactgctag
    10861 ctacgaggga gataacatga agttatctgc taaagataca ggacttcatg gtgcaagtat
    10921 ttacatggat acggttagtg tgggagcaac gattaatacg atgattgctg cggttaaagc
    10981 aaatggtcgt actattattg aaaatgcagc ccgtgaacct gagattattg atgtagctac
    11041 tctcttgaat aatatgggtg cccatatccg tggggcagga actaatatca tcattattga
    11101 tggtgttgaa agattacatg ggacacgtca tcaggtgatt ccagaccgca ttgaagctgg
    11161 aacatatata tctttagctg ctgcagttgg taaaggaatt cgtataaata atgttcttta
    11221 cgaacacctg gaagggttta ttgctaagtt ggaagaaatg ggagtgagaa tgactgtatc
    11281 tgaagacagc atttttgtcg aggaacagtc taatttgaaa gcaatcaata ttaagacagc
    11341 tccttaccca ggctttgcaa ctgatttgca acaaccgctt acccctcttt tactaagagc
    11401 gaatggtcgt ggtacaattg tcgatacgat ttacgaaaaa cgtgtaaatc atgtttttga
    11461 actagcaaag atggatgcgg atatttcgac aacaaatggt catattttgt acacgggtgg
    11521 acgtgattta cgtggggcca gtgttaaagc gaccgactta agagctgggg ctgcactagt
    11581 cattgctggg cttatggctg aaggtaaaac tgaaattacc aatatcgagt ttatcttacg
    11641 tggttattct gatattatcg aaaaattacg taatttagga gcggatatta gacttgttga
    11701 ggattaaacc gtagaggtgt ttatgaatat ttggaccaaa ttagcaatgt tttctttttt
    11761 tgaaacggat cgcttgtatt tgcgtccttt cttttttagt gatagtcagg acttccgcga
    11821 gatagcttca aatccagaaa atcttcaatt tattttccca acgcaggcaa gtctggaaga
    11881 aagtcaatat gcactggcca attactttat gaagtcccct ttgggagtgt gggcaatttg
    11941 tgaccagaaa aatcaacaaa tgattggttc tattaaattt gagaagttag atgaaatcaa
    12001 aaaagaagct gagcttggct attttttgag aaaagatgct tggtcgcaag gatttatgac
    12061 agaggttgtt agaaaaattt gtcagctttc ttttgaggaa tttggcttaa aacaattatt
    12121 tatcattacc caccttgaaa atgaagctag ccaaagagtt gctcttaagt ctggatttag
    12181 tttgttccgt cagtttaagg gaagtgatcg ttacacaaga aaaatgcggg attatcttga
    12241 atttcggtat gtaaaaggag agttcaatga gtaagcatca ggaaattcta agctatttgg
    12301 aggaattacc agtaggtaaa agggtcagtg ttcgtagcat ttcgaatcat ctaggagtta
    12361 gtgatggaac agcctatcga gctattaaag aagctgaaaa ccgtggaatt gtggagaccc
    12421 gtcctagaag tggaacaatt cgtgttaaat cccagaaagt tgctatagag agattaacgt
    12481 ttgctgaaat tgcagaagtg acttcttctg aggttctggc tgggcaagaa ggtttagaga
    12541 gagagtttag taagttttca attggtgcca tgactgaaca aaatatcttg tcttaccttc
    12601 atgatggggg gctcttgatt gtcggagacc gaacccgtat tcagttgcta gccttggaaa
    12661 atgaaaatgc agttctggtt acagggggat ttcaggttca tgatgatgtg cttaaactgg
    12721 ccaatcaaaa agggattcct gttctaagaa gtaagcatga tacctttacc gtcgcgacca
    12781 tgatcaataa agccttgtca aatgtccaaa tcaagactga tattctgaca gttgagaaac
    12841 tttatcgccc tagtcatgag tatggttttc tgagagagac agatacagtt aaagattatt
    12901 tggacttggt tcgtaagaat cgtagcagcc gtttccctgt tatcaatcaa catcaggtcg
    12961 ttgttggtgt tgtaaccatg agagacgctg gtgataaatc accaagcacg acaattgata
    13021 aggttatgtc tcgtagtcta ttttgggttg gattatcgac aaatattgcc aatgtgagtc
    13081 aacggatgat cgcagaagac tttgaaatgg taccagttgt tcgaagcaat caaactttgc
    13141 ttggcgttgt gacgcgacga gatgtcatgg agaagatgag ccgttcccaa gtttcggctc
    13201 taccaacttt ttctgagcag attggacaaa agctctctta tcaccatgat gaagtagtca
    13261 ttacagtgga accctttatg ctagaaaaaa atggagtttt ggctaatggt gtattggcag
    13321 aaattctgac ccacatgacc caagatttag ttgttaatag tggtcgcaat ctcattatcg
    13381 agcagatgct gatctacttt ttgcaggctg ttcagataga tgatatattg cgcattcagg
    13441 cacggattat tcatcatacg agacggtcag ctataattga ttacgatatt tatcatggtc
    13501 accagattgt ttcaaaagca aatgtgactg ttaaaattaa ttagaaacta ggagaaaaga
    13561 tgataacatt aaaatcagct cgtgaaatcg aagctatgga caaggctggt gattttctag
    13621 caagtattca tataggctta cgtgatttga ttaagccagg cgtagatatg tgggaagttg
    13681 aagaatatgt ccgccgtcgt tgtaaagaag aaaatttcct tccacttcag attggggttg
    13741 acggtgccat gatggactat ccttatgcta cctgttgctc tcttaacgat gaagtggctc
    13801 acgctttccc tcgtcattat atcttgaaag atggtgattt gctcaaagtt gatatggttt
    13861 tgggaggtcc cattgctaaa tctgacctaa atgtctcaaa attaaacttc aacaatgttg
    13921 aacaaatgaa aaaatacact cagagttatt ctggtggttt agcagactca tgttgggctt
    13981 atgctgttgg tacaccgtcc gaagaagtca aaaacttgat ggatgtaacc aaagaagcta
    14041 tgtacaaggg tattgagcaa gctgttgttg gaaatcgtat cggtgatatc ggtgcggcta
    14101 ttcaagaata cgctgaaagt cgtggttacg gtgtagtgcg tgatttggtt ggtcatggtg
    14161 ttggcccaac tatgcacgaa gaaccaatgg ttcctaacta tggtattgca ggtcgtggac
    14221 tccgtcttcg tgaaggaatg gtcttaacca ttgaaccaat gatcaataca ggcgattggg
    14281 aaattgatac agatatgaaa actggttggg cgcataagac cattgacggt ggattgtcat
    14341 gtcagtatga acaccaattt gtcattacga aagatggacc tgttatcttg actagccaag
    14401 gtgaagaagg aacttattaa taaaaagtga aaagactact ggaagtttat tttgataaaa
    14461 aatccagtag atcttttcat aataaaacgc attgtatcaa gtgttagggg ctgatatcat
    14521 gcgtttttct gcttttaaga ttttttccaa ctctgtttgt aagcgcatca taacaaaggg
    14581 tctaggattc agggctctcc tcctatatac tattagtaaa gtaaaactaa gggaggatat
    14641 tttagtgtcg cagtctattg ttcctgtaga gattccacaa tattgtcgtt ttgattctaa
    14701 aaagagaaat ggaattctgt ttaacgttcg tattgccaat cttaaattta ccttttttag
    14761 attagacttc ctgcgaaata aaatatggta tagtagttct atgaatgatg aagcaagtaa
    14821 acaactaact gatgcaggat ttaagcgtct tgtt
//
LOCUS       DAVMEI010000033        14773 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVMEI010000033 DAVMEI010000000
VERSION     DAVMEI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.033"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(184..1806)
                     /gene="groL"
                     /locus_tag="VYQ24_000810"
     CDS             complement(184..1806)
                     /gene="groL"
                     /locus_tag="VYQ24_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1967975.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISNRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1822..2106)
                     /gene="groES"
                     /locus_tag="VYQ24_000811"
     CDS             complement(1822..2106)
                     /gene="groES"
                     /locus_tag="VYQ24_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1967976.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2376..2771)
                     /locus_tag="VYQ24_000812"
     CDS             complement(2376..2771)
                     /locus_tag="VYQ24_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1967977.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(2848..3609)
                     /locus_tag="VYQ24_000813"
     CDS             complement(2848..3609)
                     /locus_tag="VYQ24_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1967978.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTHCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(3642..4268)
                     /locus_tag="VYQ24_000814"
     CDS             complement(3642..4268)
                     /locus_tag="VYQ24_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1967979.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVACLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPVKHWTA"
     gene            complement(4284..4601)
                     /locus_tag="VYQ24_000815"
     CDS             complement(4284..4601)
                     /locus_tag="VYQ24_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1967980.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(4598..4897)
                     /locus_tag="VYQ24_000816"
     CDS             complement(4598..4897)
                     /locus_tag="VYQ24_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1967981.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            4985..5065
                     /locus_tag="VYQ24_000817"
                     /pseudo
     CDS             4985..5065
                     /locus_tag="VYQ24_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(5194..5385)
                     /locus_tag="VYQ24_000818"
     CDS             complement(5194..5385)
                     /locus_tag="VYQ24_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1967982.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(5546..6220)
                     /locus_tag="VYQ24_000819"
     CDS             complement(5546..6220)
                     /locus_tag="VYQ24_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967983.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKIVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6226..6672)
                     /locus_tag="VYQ24_000820"
     CDS             complement(6226..6672)
                     /locus_tag="VYQ24_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1967984.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(6786..7289)
                     /locus_tag="VYQ24_000821"
     CDS             complement(6786..7289)
                     /locus_tag="VYQ24_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1967985.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVTLSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7286..7648)
                     /locus_tag="VYQ24_000822"
     CDS             complement(7286..7648)
                     /locus_tag="VYQ24_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1967986.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(7714..9573)
                     /locus_tag="VYQ24_000823"
                     /pseudo
     CDS             complement(7714..9573)
                     /locus_tag="VYQ24_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(9612..10061)
                     /locus_tag="VYQ24_000824"
                     /pseudo
     CDS             complement(9612..10061)
                     /locus_tag="VYQ24_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            complement(10185..10901)
                     /locus_tag="VYQ24_000825"
     CDS             complement(10185..10901)
                     /locus_tag="VYQ24_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1967987.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(11782..13197)
                     /gene="ply"
                     /locus_tag="VYQ24_000826"
     CDS             complement(11782..13197)
                     /gene="ply"
                     /locus_tag="VYQ24_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1967988.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13208..13540)
                     /locus_tag="VYQ24_000827"
     CDS             complement(13208..13540)
                     /locus_tag="VYQ24_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967989.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTGIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(13611..14219)
                     /locus_tag="VYQ24_000828"
     CDS             complement(13611..14219)
                     /locus_tag="VYQ24_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967990.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14232..14690)
                     /locus_tag="VYQ24_000829"
     CDS             complement(14232..14690)
                     /locus_tag="VYQ24_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1967991.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         4291 a         3421 c         2686 g         4375 t
ORIGIN      
        1 aactttgaaa acttcaaaaa acggattttc atcgctctga atatcaaaaa agaaaggaca
       61 aaatttgtcc tttctcgagc ttagcttttc ttcaacccac tacagttgac aaagagccta
      121 taaaaagaag ggttagtcat tccctccttt tgtgtttttt ataagttgtt ttctatagaa
      181 agcttacatc atcccgccca tcatacttgg atccattgct ggagctgggg ctactggttc
      241 tggtttattg gctacgactg cttctgttgt caaaatcaag ctggctacag atgctgcatt
      301 ttgtagggct gaacgactca ctttaactgg atcaatgata ccttgatcaa tcatgttaac
      361 ccactcgcca gttgctgcgt tgaatcctat accaagctca gcatttttca aacgatcgat
      421 aacgatagat ccttcaaatc ctgcattgtg agcaatttga cgaacgggtt cttccaaagc
      481 acggagaaca atattacgtc ctgttgcttc atctcctgtc aattccaagg tagcaacagc
      541 tggaatcaca ttggcaagag ctgttccacc acctgcaaca ataccttctt caacagctgc
      601 acgagtagcg ttgagggcat cttcaatgcg gagtttcatt tctttcaact cagtttcagt
      661 tgcggctccg accttaataa ccgctacacc acctgacaat ttggccaagc gttcttgcaa
      721 tttttcacgg tcaaattcag aagttgtagt ttcgatttga gacttgataa ccgcaacacg
      781 gttagaaatc gcttcaggat ttcctgcacc ttctacaata accgtgctat ctttgtccac
      841 ggtcactctc gctgcttgac caagagcttc aattgtcgca tctttcaact caagaccaag
      901 gtcttctgtg ataactgttc cgcctgttaa gatggcgata tcttcaagca tggctttgcg
      961 acggtcacca aaaccaggtg ccttgactgc tactacgttg aaggttccac gaatcttgtt
     1021 caaaacaaga gttggaagag cctcgccatc cacatcatcc gcaataatca agagtggacg
     1081 attgctttgg agaatgcttt ccaaaagtgg caagatttct tggatattgg aaattttctt
     1141 gtctgtaatc aaaatgtacg gattttcaag gtcagccacc attttttcgc tatcagtcac
     1201 catgtactgt gaaaggtaac cacggtcaaa ctgcattcct tctacgactt caagctctgt
     1261 ttccatacca cgtgactctt cgatggtgat gacaccgtct ttgccaactt tttccattgc
     1321 ttcagagatg tactcaccaa ctttttcaga acgagaagat acggctgcaa cttgagcgat
     1381 agcttcttta ttggcaacag ggatggcgtt gtttttcaaa gcttcaactg ctgcggcaac
     1441 tgctgtttca atcccacgac gaataccgat tggatttgca cctgctgtga cgtttttgat
     1501 tccttcacgg acgatagctt gggtcaagac tgttgcagtc gtagtcccgt cacctgcgat
     1561 atcattggtt ttagaagcta cttctgatac taacttagca cccatatttt caaaatggtc
     1621 ttccaattcg atttctttgg caatggtcac accgtcattg gtaatcaagg gtgaaccgaa
     1681 tgacttttca agaacgacat tgcgaccttt tggtcccaag gttactttaa cagtgtctgc
     1741 aagaatatcg acaccacgaa ccatggctga acgggcatct gatgaaaatt taatttcttt
     1801 tgacatactt actttctcct tctattcctc aatgattgcc aaaatgttag cttcgcctac
     1861 gataatgtac ttttcatcgc catctttgac atcaagacct gcgtgggctt caactaagac
     1921 acgatctcca gttttaacac ttggagcaac caagtcaccg ttcaaggtac gaacaccttg
     1981 tccagtagcc acaacttgag ctgttttggt tttttcttgg gctgagcctg caaggacaaa
     2041 gcccccaacg gtttgttctt tttcttctat ttttaagacc acacggtccc ctaatggttt
     2101 caacatctga tttcctccat aatgagatag atattattag cactctttga actggagtgc
     2161 taattcataa ttctattgta tcacttggtc agaaatagtc aagaaaaaag tctgactttc
     2221 tcaagataaa aagcctgaga ccaactcaga ctttttaatg tttatactca atgaaaatca
     2281 aagagcaaac taggaagcta gccgcaggct gctcaaagca ttgctttgag gttgtggata
     2341 aagctaacgt ggtttgaaga gattttcgaa gagtattaaa atggcaattc ttcctcttcc
     2401 aagaccaaat ctgccaaatc ttggcctgca ttattttcac gcatggcacg ttgagcacga
     2461 ctttctaaga gttggaatcc tgtcacaagg acttcggtta catagttcat ctgaccattt
     2521 ttctcaaagc gacgggtacg caattctcca tcaacggaaa tgagactacc tttggttgcg
     2581 tagcttgcca aagtttctgc tagtctgccc cataggacca tattgacaaa atcagcttca
     2641 cgttcaccgt tttggtcttt gtaacgacgg ttgacagcga tagttgctcg cgctaccgac
     2701 ttgtcattgt tggttttgtg caattctggt gtagacgtta aacgcccaat catgataact
     2761 ttattataca tattttcttc ctcctactta tctattcgta ggaaatcaaa aaagttacag
     2821 aaatttgtaa cttttcgagg aaatttctta ttttttatga atcatgaaac ctgtcgcctg
     2881 ttgattggcc ataatggtca tatctgtaat ctgaacacgg cgaggctgac tggtcacata
     2941 gactactgtg tctgcaatat cctgagcttg caaggcttct attccttggt aaacggacgc
     3001 agcccgctct ttatcaccat gaaaacgaac agttgagaaa tctgtttcga caatcccagg
     3061 ctgaatggtt gtcaccttga tatccgttgc gatggtatca attcgcagtc catccgaaaa
     3121 ggtcttaacc gctgccttgg tagctgagta aacagcggca ccagcatagg cgtagattcc
     3181 tgcggttgac cccatattga taatatgacc ttgattgact tttatcattg ctggcaagaa
     3241 acagtgagta accgccatca gacctttaac attggtatcc aacatggtca acatatccaa
     3301 ctcctcatag tcttgatagg gagccaagtc aagagccagt ccggcgttgt tgaccaagat
     3361 atcaatcctc cctatcgttt ctagaatatc agagcaaaca gtcttcacca tgaccatatc
     3421 cgtcacatct agtggaaaag tccagacggt ttgatttgga aagcttactg caaactccga
     3481 cttgaggatt tctaatctgt ctatccgtcg tcctgttaga acgacatcct caccctgctc
     3541 cagataagca cgcgcaatcg cttcaccgat tcctgaggtt gctcccgtaa tcactacatt
     3601 ttttgccatc ttatttcctt ctatcagatg ctgacaagtt cctaggcagt ccagtgtttc
     3661 actgggtcga acggtgttcc gacaacttgg tcttctgata attcaagcac cccacgtttt
     3721 tgcggagcat ttggcaaatg caattcacga ggactacaca tcatcccaaa actcttttca
     3781 ccacgaagtt cgcctgggaa aatgaaattc ccttttggca tcatggcacc aggaagagcc
     3841 acaatggttt tcaacccaac acgcgcatta ggtgctcctg caacgatttg cactatcttg
     3901 tcacttgcga ctgcaacttg gcagatgttg aggtggtcac tatctggatg ggctaccatc
     3961 tcaacaattt caccaacaac aaacttaggt tccttgtcat tgacaatttc ttctgtaaaa
     4021 ccttccgcct gtaattcttg gttcaaacaa gcgacttgct catctgataa aaagacttga
     4081 cctcgctctg caatttcaaa caagctcgaa acctcaaaaa tgttccaagc tactgtttcc
     4141 ccattttctt tgaggaaaac acgggctacc ttgcctttgc gttccacatt cagtttggca
     4201 tctccgctat ttttcacgat gaccataagg acatcaccga catgttcttt gttatatgta
     4261 aaaatcattg tttacttttt ctcctatttc aatcctgcta aaaaatcgtt aatttgctcc
     4321 ttacttttac ggtcgcgatt gacaaaacgg ccgatttcct tgtccttttc tagaacaaca
     4381 aggctaggaa ttccgtaaac atcccagagt ttggccaaat ccatatactg atctcggtcc
     4441 attcgaataa aggtgaactc tggattggtc tcctcaatct ctggtaaggc aggatagata
     4501 taacggcaat cgccacacca gtccgccaca aaaaggaaga ccttcttacc gtctttttct
     4561 actaaagatg ccaattcttc taaacttgct ggttgtatca taagacttcc tcctcataga
     4621 ctaggtcttc attttcatag acaaaagtat aatgacggcc atcctcaaaa atgacgccac
     4681 caagcaagct ctctagactg ctttcataga cttgaacata gagggtcgca atttccccca
     4741 tgtctgaaaa atggtctcgt acaatcgctg tcaactcttc ctgagtcttc atgagcttat
     4801 ggtcatctgc aacttttttc gtagcaaggg caaggctccc aataccaagc agagccagac
     4861 ctgccatcca cattttttta gctttcatac cattcatttt aacacaaaaa aggctttagg
     4921 acaaatgagg aaacagcaaa aaagcaagta aaaagcctct tcctttaagg aaaaggactt
     4981 cttatactca atgaaaatca aagaccaaac tagaaagcta gccgcaggct gctcaaaaca
     5041 cggttttgag gttgtagata aggcgacgct gacgtggttt gaagagattt tcaaagagta
     5101 ttattcttat tgccaggcac ctaagttgcc aacgtagtaa ccatcaggtg tgtaggtatt
     5161 gcgaagcatc ttacctgatg aagccagata atactacttg ccattgtctt tgacccaatc
     5221 attcgcaatc atggaaccag aagaacttac ataataccat tctcccttgt cataaaccca
     5281 agtactgact ttcatggttc ctgagcaatt aaaggcaaaa aaactgtcca atatcattcg
     5341 ttttttaaaa gcatttgaca ctacatcatc tacgctgtag tacatagata catcaaacgt
     5401 aaaagccaaa atatcgaaaa atatacaact tttaaaggca gcatttcatt ttttaaagtc
     5461 taacaaccat agaactgaaa tcagagcaag gcatcaattt cctactctta gtacgtctat
     5521 tttgatggtt gaagtgaaga tttttttatt taacgtagac aatttccaac ttcccaaaag
     5581 ccacatctcc acggatagtc aaggttttgc tggttggggc tacaggagcg tcagccttag
     5641 ctgcaccaaa ggaggtttct actttcaaat ctacacgcca gtgttgtgga acatagacgg
     5701 ttgcatttcc gaaggccact tcaatattta aagttgcaaa atcacctagc atctctgcat
     5761 tatcatagta gattttagca tccccaaaag cgacttccac ttggtcatct acgagatctt
     5821 gatcttgctt atagaaggtc ccgctaccaa aagcgacttc cttatccgtg acgactattt
     5881 ttttcccatt gtaccaccat tttttttcat tccagaactt acttgaatgc gtcagataac
     5941 caacaccaag taccaccaag atgctagccc aaataagaga atgattggta actggtaaca
     6001 agtcataagc gtaatttgca atgatgagcg ccagtaaacc tgtaaaaact gccgaagtga
     6061 gatgacgtct aaggatggac tcaatggact tataagcaaa aaaaacaata cctagtaaag
     6121 gccatatttt accatccaaa gaaggaattc caaaattccc ttgcagcaag atccaagctg
     6181 ctaaaaccaa taaaacaata ccaaatgctt tctttttcat gttttttacc tcatgtttct
     6241 tagattttct tttaggaggg attggtaatg ccgtgagaca tgaacctcct tgtgcgtctg
     6301 ataaaaggaa atggtgcccg ttcctgaaaa ggacttgtcc actgagtaaa tctgacggat
     6361 gtttgcgatc gttgacttgg aaactcgatt aaaatagcga ggcaagatag actccaactc
     6421 atagagcttg aggcgaacct cataagcttc cttctggtta tgagcgtaga tcttgctccc
     6481 ttctgtttca aagaagagaa tttctgacag gtctagataa tattcacctg tccccttgta
     6541 aaaagtcaac ctcggagcct ttgactcttg caagagccgt tgcaagtctg caatttcatc
     6601 tgtcaaagtg ggggtcttga tgacaatttc agtctcctct aaattgccgt caatctcaat
     6661 tcgtaacttc atcacatctc ctttctgact ctactatatc aaagctaagt taggaataca
     6721 agggcaaaaa agcaagtggt caatttgaag cagtaagtgg ttgtaggact agcttaactt
     6781 acatgtcaga ataggaagaa aaggaatccc cacaccaaac cacaagcata gccaaccaag
     6841 acatccttgg gataatgaac accccctaaa accctcacca accccagcaa agctgaaaag
     6901 atcaagcata ctaagccaat aggtagacta gcatgcagac aggccatgga gatgatagtt
     6961 gccgaaaaga catggcgact gggcatcgac cgtcccgaac tatccttatc aagaagggga
     7021 cagatatccc aagtttcata aggtctcggg tgattgattt tcttacgaaa cagggacaag
     7081 gtcacaaaac ctgatgcagg aataagcaca taaaccccaa cttgctgacc aagtcctagt
     7141 tgcaggtaag tggtgactag caagatcaga tagaccaggg gcatggcgac tgtcatcaaa
     7201 cgattgaaac tgcgtaatag gaataaaaga attggacgac tagttagtct ggagctgata
     7261 tttcgatacc attcttgata atttttcatt tatgcttctc atacttttct agaacttgtt
     7321 gagatgtttt ccccttggca agccaatcca ctagcttgtc caagcgacgt atttcctgca
     7381 tcagaggatc atcaatttcc tcaatacgga caccgcaaat cttccctgta attgcaattc
     7441 gttcagggac ataatatggt gcttgtctaa aaaaatctcc gtaagtaaca tccctatcca
     7501 gacaagcaag aacatcactc acttcatacc ctgtcaacca actggtcact tggtgaactg
     7561 actctgcctt tccacccttt cgctcaactt ttgcaatcaa ggctaagtag acagcagcaa
     7621 atttcatatt gtataatttc tgactcatac ttctctcttt ctatgtgtac tagtataaat
     7681 aagaaaaaga aggccgtcaa gccttctttt gatttattct tctgcttcat cttctgtaaa
     7741 ttgactattg tacaagtcag cgtagaagcc accttgcgcc atcagttcct catagttgcc
     7801 ttgctcgatg atatttccat ctttcatgac caagatcaag tctgcatttc ggatggttga
     7861 caagcggtgg gcaatgacaa aggatgtgcg tcattccatc aaacggtcca tggctttttg
     7921 gatcaattcc tctgtccgtg tgtcaacaga agaagtcgcc tcatccaaaa tcaaaagcgg
     7981 tgcatcctta agaagggcac gagcaatagt caatagttgt ttttgtccta cagacaaggt
     8041 cacggtgtca tccaagatgg tatcatagcc atctggcaag gtcataataa agtggtgaat
     8101 tcccacagcc ttactagctt ccatcattcg ttcatcacta atccctattt gattatagat
     8161 gagattgtct cgaatagttc cttcaaagag ccaggtatcc tgcaagacca ttgaaaaggc
     8221 atcatgcact tctgaacgcg tcatagcctt ggtatccaca ccatcaatgt gaatacttcc
     8281 cttatcaatc tcatagaatt tcatcaaaag attgacaatg gttgtcttac cagtcccagt
     8341 cggcccaaca atggcaacct tttgaccagc atgagctgtc gcagagaagt catagtcttg
     8401 aacattgaca ccgtccacca gaatttctcc tgctgacacg tcgtagaaac gtggaatcag
     8461 attgaccaga gttgatttac cagaacctgt tgacccaata aaggccactg tttgaccagt
     8521 ttctgcttta aagctaacat gttcaataac tgcctccgaa tttgccgcat agcggaaggt
     8581 cacatcctta aactcgacct gacctttgaa gttttcatca gtcagctgca cttgaacagg
     8641 gttttggata gaagaatgca aatctaaaac ttgattaatc cgcttagcag agaccatagt
     8701 tcggggaaga acgatgaaga gtgctcccat gagaaggaag cccatgacaa cctacatggc
     8761 ataagacatg aaaacaatca tgtcactaaa gagaggcaga cgcgctatcg aagcagcgtc
     8821 gttaatcaca taggccccaa tccagtaaat cgctacactc aaaccacttg aaatccccat
     8881 catgatagga ttcaaaatag ccataagacg gttgacaaac aaattcaaac gggtcaattc
     8941 atcatttact gctgcaaatt tttcattttg ataatcctct gcattgtagg cacgaacgac
     9001 acgaatacct gttaaactct cacgagtgat actgttcagt ttatctgtca acccctgaat
     9061 caaggactgt tttggaaagg ctagcgtcat caaaacggtc gtcatcagga cgttgacaat
     9121 cactaccaca agtacggccc agagtcagta ttctgaatga cctaaaatct tcccaatagc
     9181 ccagatagcc ataattggac cacgcgttac cacttgcaag cccatagtaa tcaacatttg
     9241 aacttgagta atgtcattgg tagtacgcgt caagaggcta ggaattgaaa atttcttaat
     9301 ctctgtctgc gagtaatcca aaactcggtt aaaaatatca cttctcagcc tactagtata
     9361 agaagccgcc actcgggatg caaaaaatcc aactgcaact acggacaaga aggcaagaaa
     9421 gagcattccc atcatcatgc ttgccggctg ccacaactca tctaaattag tttcttgact
     9481 acctagcaaa tccgtaattt tcgagatata ggtcggcact tccaactcta gatagaccga
     9541 aaagcaagta aagagaatgg ctagtaaaat catcccccat tcttttctac taattctttt
     9601 ggttaatttc tttattctct cctcctattc ccttgatatt ttgcctgtag ttgaccgaga
     9661 actttctcaa aaatcagtaa ttcatcttca tcaatgtctt ccatcaactg cttgtctatg
     9721 cgttcaaaaa aagccttaac ctgttgcatc tgagaacgtg ctttgtccgt cagacgaaca
     9781 aacttagccc gcttatcaac aggactcgcc tccaattcca ccaaaccatt ttgcactata
     9841 cgcttaacca gattactagc aacagacttg gtaatattga gttcctgctc gatatcttta
     9901 atcaagacca agtcttggtt tttctagcga ttatccaaaa aacgcacaac ctgaccttgc
     9961 ggcccaccca taaattcaat gccgcaacgt ttggcttcct tttgcaccat caggtgaatt
    10021 tgatgaccaa aacgcttaaa gactaacatc ggtttatcca taatctcccc cttctaaata
    10081 aaaatagttc tctggagaat aattaaattt ctatgagaac tattttcttg attaaaaaaa
    10141 tcccaagtga ttttctcact taggatcatg ttctataggt taaattaaaa cccatctacg
    10201 ttcgtataaa tcttttggac gtcttcgtcg tcttcaagaa cgctgtaaag tttttcaaag
    10261 gtttcaaggt cttcgcctga caattccact tctgactgag gaatcatttc caattcagtc
    10321 acttggaatt cttcaatacc agactcacgg agagcaacga tagccttgtg aaggtcagtt
    10381 ggcgctgtgt aaactgtgat tgtaccttct tgtgcttcta cgtcatccac atccacatcc
    10441 gcttcgagca attgctcaaa gactgcgtct gcatcttcac ctccaaatac aataacacct
    10501 ttgttgtcaa agaggtaaga aacagaacct gaagcgccca tgtttccgcc gtttttacca
    10561 aaggctgcac ggacattggc tgctgtacgg ttgacgttag aagtcaaagt atccacaatt
    10621 agcatagagc catttggccc aaaaccttcg taacgtcctt ctgtaaaggt ttcgtctgtg
    10681 tttcctttgg ctttatcaat cgctttatcg ataatgtgtt ttggcacttg ggcttgttta
    10741 gcacggtcga taacgaattt caaagctgag tttgattctg gatctggatc acctttttta
    10801 gctgctacat agatttctac accaaatttt gcatatacct tagagttagc tccatcttta
    10861 gccgttttct tggctacgat attggcccat ttacgtccca ttaggaatct ccttttttca
    10921 cattttaatc tttattatta taacacaagt ttttttgatt ttcactagag gaaatggatt
    10981 ttattagcaa atcaagctag gatagcactt tacctgctaa gatggtctta cctttctatc
    11041 tttatcaaca gacactcatc cacattcaaa aaacaaacta gaccattatc tgcaaataga
    11101 aagtttcagc caagtttgac aaagtcagct caaattactg tttgaagttt gtagatataa
    11161 gcgacaaaaa caatcatact gcaccttttg ttgacagtct actccagaca tatcatagtt
    11221 caagtaaata ctttgaaatt caacagttct tataggcgct attgtattct aagaaatcaa
    11281 tagaagagtt tctaagcaaa cctctaatac tcaataaaaa tcaaagagca aaccagaaag
    11341 ctagccgcag gttgctcaaa acactgtttt gaggttgcgg atggggctga catggtttga
    11401 agagattttc gaagagtata atttacgtgt tcccaagatg gagaagttag actagtacac
    11461 tggcacttct aaaccattgc tagcaattga tttgttcata tttaatttca ttttttccat
    11521 aaatgggtat tagatataaa cagcaaaata tttccgatac gtgtcgttct tgaatttcca
    11581 atcatctaaa acaagtaaag gataatcaat cccctgtata tcaaggaatt ggctaccctt
    11641 tttacttttt tacacattct gtttgataga ttcattttaa catcacgagc atactccaat
    11701 ggaaatcgct aggcaagaga taaactttca gatatccgca gagagatcat cgcctctttt
    11761 tgtcgcaagc attctcctct cctagtcatt ttctacctta tcttctacct gaggatagag
    11821 agttgttccc caaatagaaa tcgtccgctt acgcactagt ggcaaatcgg ttttttcata
    11881 aaccgtacgc caccattccc aggcaagccc ggtacactct ctaattttga cagagagatt
    11941 acgaacattc ccttttaaag gaatactagt ggtaaagtga gccgttaaat cctgcccatt
    12001 tctgtcccaa gccttaggag tcaagacttc cttaccttga tgatcatagg ataattcatt
    12061 ccaagtaata taatattggg caacataggc accactatga tccagcagta aatctccgtt
    12121 tctgtaagct gtaaccttag tctcaacata gtctgtacta ttttgaaagg tcgcaactac
    12181 attgtcacgt aaaaaagaag ttgtatagga aatcggcaag cctggatgat ctgctgtaaa
    12241 gcgactgcct tcttgaatca agtcctctac catatccacc ttgcctgtta caactcgggc
    12301 acccgaactt gggtcgcccc ctaaaataac cgccttcact tctgtattgt ccaaaatctg
    12361 tttccactct gtctgaggag ctaccttgac tccttttatc aaagcttcaa aagcagcctc
    12421 tacttcatca ctcttactcg tggtttccaa cttgagatag acttggcgcc cataagcaac
    12481 actcgaaata tagaccaaag gacgctctgc agaaattcct ctctgtttta aatcctctac
    12541 cgttacagta tcttgaaaca catctcctgg atttttaaca gcatctacgc tgactgtata
    12601 ataaatctgc ttaaaattaa caatctgaat ctgcttttcg cccgaatgga cagagttaaa
    12661 atcaatatca agagaattcc ctgtcttttc aaagtcagaa ccaaacttga ccttgagttg
    12721 ttccatgctg tgagccgtga ttttttcata ctgcattcta gctgggacat tattgacctg
    12781 accataatct tgatgccact tagccaacaa atcgtttacc gctccgcgaa cacttgaatt
    12841 gctggggtct tccacttgga gaaagctatc gctacttgcc aaaccaggca aatcaatact
    12901 ataagtcatc ggagcacgat cgaccgcaag aagagtggga ttattctcta acaaggtctc
    12961 atccactacg agaagtgctc caggatagag gcgactgtcg ttggtagctg ttacagaaat
    13021 atcacttgta tttgtcgaca agctccgctt ctttctttcg ataacaacaa actcatcggg
    13081 tagctgatta ccctctttga tgaaacgatt ttcaatactt tctccctgat gggtcaagag
    13141 tttcttttta tcgtaattca tagctagtat aaagtcattt actgctttat ttgccatctt
    13201 ctacctccta ataagttcct ggattgagtt gcataaactc agacttgttc agcgaaatca
    13261 gccgtggttg gactaagtaa tccaaaattt cctcgtacaa ttcttctgag acattgcgtc
    13321 gccgtctggc tagataagaa gtcggaatgc ccgtattatc caacataaat accttatcta
    13381 agtcaatcaa ggttggtctt gtaaaaggat tacgagctag atccggctct tctatcataa
    13441 agttcttgac caaacgtctg gtcaagagag ctggtttgaa ggtctgattt ttaaccaact
    13501 ctttgttttt agtcatgctg ttgtcaatac agatatacat atgattcttc acagccaaat
    13561 cgctactaat agtcggaaaa ggcaaataaa gagctacaac atctcctctc ttaatcaagc
    13621 aagagcaccc ccttttctcc taatgtaaca tagacaggat tgaccaagtc ttctgattga
    13681 ctcagaattt ccaaagtttg agtttggcgc gctgtcaatt tagtagcatc ttgtctcttc
    13741 aatacaaaat gcttgtcgcc aataaccttg acaatataat ccttctccaa agctgactgg
    13801 taaatccaca tcagatgttg tctgtcctga gaactcaaga gagaaggatt ttcaagcctc
    13861 ccgatagtct gataaaaatc aaaaacagga gctaactcct gccaatctga ttggctagtt
    13921 gtcaaggcta gaaaaagggc tttgcgagct gatacttctt ggttagcctt gagagttact
    13981 ttcccctcca agttttttag aaatcgggaa actccagaaa gcaaattttt ctctaactgc
    14041 gagaaataaa aacctttcgt tcccagacat aagtctttca tgtcgctttc tctagcaaat
    14101 aagagctcaa acatttgata gtaaaagaaa aatatctggc actgggtcgc gctcatcttt
    14161 tccttatcgg cttctttttt taaccagagc aagggcgaca ggtagctgga ttgagacatt
    14221 tcctctacct cctactcttt tttaactgga gcatctgcac tagctgccac ttcttttgac
    14281 tggatacttt cccactggtt aatctcctct gagataagac cttcgcatgt cttgacaaat
    14341 agggcaaaag ccttggtctt tcctgcatat ttctccgttt ggcattgata gaggaatttt
    14401 tctttctcca ggagttgcgc agttttttgg taagaaatcc aattttcctt tgcattatac
    14461 aaattgataa tcccctcaca cagcaagccg agactggata aggcaaccga aatcaaacgg
    14521 tagcgatcac ctggcatagg aatagcacaa aagacagcta tgaggaaacc tgccacgatt
    14581 tctgttattt ttaatacctt atagcgtcta cgatgttgaa cgcttttctt taaaaaatga
    14641 gctatctgta cgtctaatcg ctctgtcagg tacatttctt ctggcgtcat attcgtaact
    14701 cctttcattt actttgataa ccatagtata acatatttga atatcaaagt atagtaaaaa
    14761 ctggactata act
//
LOCUS       DAVMEI010000034        14706 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVMEI010000034 DAVMEI010000000
VERSION     DAVMEI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14706
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.034"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(113..862)
                     /locus_tag="VYQ24_000830"
     CDS             complement(113..862)
                     /locus_tag="VYQ24_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1967992.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDCSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            995..3331
                     /locus_tag="VYQ24_000831"
     CDS             995..3331
                     /locus_tag="VYQ24_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1967993.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKLINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHNYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            3433..4110
                     /locus_tag="VYQ24_000832"
     CDS             3433..4110
                     /locus_tag="VYQ24_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1967994.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            4097..4894
                     /locus_tag="VYQ24_000833"
     CDS             4097..4894
                     /locus_tag="VYQ24_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1967995.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            4911..6014
                     /locus_tag="VYQ24_000834"
     CDS             4911..6014
                     /locus_tag="VYQ24_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1967996.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARVTSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            6021..6212
                     /locus_tag="VYQ24_000835"
     CDS             6021..6212
                     /locus_tag="VYQ24_000835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1967997.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            6315..7334
                     /gene="galE"
                     /locus_tag="VYQ24_000836"
     CDS             6315..7334
                     /gene="galE"
                     /locus_tag="VYQ24_000836"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1967998.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            7336..8301
                     /locus_tag="VYQ24_000837"
     CDS             7336..8301
                     /locus_tag="VYQ24_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1967999.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(8352..8558)
                     /locus_tag="VYQ24_000838"
     CDS             complement(8352..8558)
                     /locus_tag="VYQ24_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1968000.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            8610..9086
                     /locus_tag="VYQ24_000839"
     CDS             8610..9086
                     /locus_tag="VYQ24_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1968001.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSHPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            9095..9766
                     /locus_tag="VYQ24_000840"
     CDS             9095..9766
                     /locus_tag="VYQ24_000840"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1968002.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            9897..10235
                     /locus_tag="VYQ24_000841"
     CDS             9897..10235
                     /locus_tag="VYQ24_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1968003.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            10238..10867
                     /locus_tag="VYQ24_000842"
     CDS             10238..10867
                     /locus_tag="VYQ24_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1968004.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            11107..12258
                     /locus_tag="VYQ24_000843"
     CDS             11107..12258
                     /locus_tag="VYQ24_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1968005.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVMPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            12571..13320
                     /gene="truA"
                     /locus_tag="VYQ24_000844"
     CDS             12571..13320
                     /gene="truA"
                     /locus_tag="VYQ24_000844"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1968006.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            13310..14083
                     /locus_tag="VYQ24_000845"
     CDS             13310..14083
                     /locus_tag="VYQ24_000845"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1968007.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            14061..14522
                     /locus_tag="VYQ24_000846"
     CDS             14061..14522
                     /locus_tag="VYQ24_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1968008.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         4082 a         2836 c         3435 g         4353 t
ORIGIN      
        1 caggacagtc aaatcgattt ctaaaaatgt tttagaagta gaggtgtact attctagttc
       61 caatctacta tataaatgaa aaccttagct gtttggccaa ggttcatttc ttctatgcaa
      121 gttcttctct tttcttatct gggttccaga tgaagcttgc gataaagcta aagatgatgg
      181 ttaggatagc gaggataata gcaaggatga gggcagggat gcggataaac caccagagtg
      241 gatttggttt tttatcattg tgccattgtt tcgtttcttc gtccagacgt tggaattctg
      301 tttgaatacg attggcgact gtttcaatgc cttcatcatt cattttcttg atatctgaga
      361 tatcgattgg atttccaaag ttcatatcga cacgttcacg gctaatcaag cccttcaaag
      421 tcatgggacc ggtgtaggta accggcatga tacggacctt ggccattttg gcaatcagtg
      481 ctgcgccccc cttgacatcg tttgagtggc ggctaccact tggaaacatg atgagagagc
      541 agtcactttt tttgagaacg ttgataggat atttgatggc tgaggcgctg ggattttcac
      601 ggtcgatggg aaaggcgcca cacatacgaa tccaccaacc aaagatacgg ttggtaaaga
      661 gttctttttt tgccataaag atgaactgtt ttggcttggt cgcaaaggcc atataaacag
      721 gatcccacca ggtacggtga ggcgcaacta aaatataatt ttcatcttga ttaggaattt
      781 tatcagtatt atgatagtga gcattgccat tgatggacca taagagcaat acaactaatc
      841 cacgcaaata agtataaaac atgcgatctc cttcaattgt tttcttgtta ttattatacc
      901 ttatcaaagg agggctggca aacttttccc ttgactagat gcatatttgg gatgaaatta
      961 gaattctgtt aaaaaaaatg atataataga atttatggat aaaaataaga ttatgggatt
     1021 aacccaaaga gaagtcaagg aaagacaggc tgagggtttg gtcaatgact ttaccgcatc
     1081 agccagtacc agcacttggc aaatcgttaa acgaaatgtc tttacccttt ttaacgcttt
     1141 gaactttgcc attgctttgg cccttgcctt tgtgcaggct tggagcaatc tggtcttctt
     1201 tgctgttatc tgctttaacg ctttttctgg gattgtgacc gagctacgag ccaaacacat
     1261 ggtggacaag ctcaatctca tgaccaagga aaaggtcaaa accatccgtg atggtcagga
     1321 agttgctctt aatcctgaag aattagtgct aggagatgtc attcgtttgt ctgcaggaga
     1381 gcagattcct agtgatgcct tggttttgga aggctttgcg gaagtcaatg aagccatgtt
     1441 aacgggagaa agtgatttgg tgcaaaagga agttgacggc ttacttttgt caggaagttt
     1501 cctagccagt gggtcagttt tatctcaagt tcaccatgtc ggtgcagaca actatgctgc
     1561 caaactcatg cttgaggcta agaccgttaa actcatcaac tcccgtatca tgaaatcgct
     1621 ggacaagttg gctggtttta ctgggaagat tatcattccc tttggtctgg ctctcttgct
     1681 ggaagccttg cttttaaaag gcctgcctct caagtcatct gttgtaaact cgtcgacagc
     1741 tcttttggga atgttgccta agggaattgc ccttttgacc attacttcgc tcttgactgc
     1801 agtgattaag ctaggtttga aaaaggtctt ggtgcaggag atgtactctg ttgagacctt
     1861 ggcgcgcgtg gatatgctct gtctggacaa gacgggtacc atcacccaag gaaagatgca
     1921 ggtggaggct gttcttccac tgacggaaac ttatggtgaa gaggctgttg ccagcatcct
     1981 gaccagctat atggcccata gtgaggataa aaatccaaca gctcaagcca ttcgccagcg
     2041 ttttgtggga gatgttgctt atcctatgat ttccaatctt cccttctcga gcgaccgcaa
     2101 gtggggggct atggagttag aaggtttggg aatagttttc ttaggggcac ctgagatgtt
     2161 gctggattct gaagtcccag aagccaggga ggccttggag agaggatcac gtgtcttggt
     2221 cttagctctc agtcaggaga aattagacca tcacaaacca cagaaaccat ctgatattca
     2281 ggctctagcc ttgctggaaa tcttggaccc cattcgagag ggagcagcag agacgctgga
     2341 ctatctccgt tctcaggagg tgggactcaa gattatctct ggtgacaatc cagttacggt
     2401 gtccagcatt gcccagaagg ctggttttgc ggactatcac aactatgtag attgctcaaa
     2461 aatcaccgat gaggaattga tggccatggc ggaggagaca gcgattttcg gacgtgtttc
     2521 ccctcatcaa aagaaactca tcatccaaac gttgaaaaaa gcgggtcata caacggctat
     2581 gacaggggac ggggttaatg atatcttggc ccttcgtgag gcggattgtt ctatcgtgat
     2641 ggcggagggg gatccagcaa cccgtcagat tgccaatctg gttctcttaa actcagactt
     2701 taatgatgtt cctgagattc tcttcgaggg tcgtcgcgtg gtcaataaca ttgcccacat
     2761 cgccccgatt ttcttgataa agaccatcta ttccttcctg ttagcagtca tctgtattgc
     2821 cagtgcttta ctaggtcggt cagagtggat tttgattttc cccttcattc cgatccagat
     2881 taccatgatt gaccagtttg tggaaggttt cccaccattc gttttgactt ttgagcgaaa
     2941 tatcaaacct gtcgagccaa acttcctcag aagatccatg cttcgtgccc taccaagtgc
     3001 cctcatggtc gtgtttagcg ttctttttgt gaaaatcttt ggaacgagtc aaggttggtc
     3061 tgagttagaa atctcaactc tcctctatta tctcttgggg tcaattggtt tcttatccgt
     3121 atttagagcc tgcatgccat tcactctatg gcgagtcctc ttgattgtct ggtcagtagg
     3181 aggcttccta gccacagctc tcttcccaag aatccaaaaa ctgcttgaaa tttcaacctt
     3241 gacagaacaa acgttgcctg tttatggtgt catgatgttg gtctttacag tgattttcat
     3301 cctgactagt cgctatcaag ctagaaaata aagaaagact gcaatctgtg gattgcagtt
     3361 tttttaggta caagattgcc agccgaaatg tggtataata agaggtaata gagttttgga
     3421 aagtgagaga agatgatttc aaagagatta gaattggtag cttcctttgt gtcacagggg
     3481 gctattttac tagatgtggg aagtgaccat gcttatctgc ctatcgagtt ggttgagaga
     3541 ggccaaatca aaagcgctat tgcaggtgag gtggtggaag gtccctatca gtctgcggtt
     3601 aaaaatgttg aggctcacgg cctaaaggag aaaatccaag tccgtttagc caatggcttg
     3661 gcagcttttg aagagactga ccaagtgtct gtcattacca ttgctggcat gggtggtcgt
     3721 ttgattgcta ggattttaga agaaggtttg gggaagttag ctaatgtaga acgtttgatc
     3781 ctccagccca ataatcgtga agacgacttg cgtatctggc tacaggatca tggattccag
     3841 attgtagcag aaagcatctt agaagaagct ggaaagtttt atgagatttt ggtggtggaa
     3901 gcaggacaaa tgaagctatc agccagtgat gttcgctttg gtcccttctt gtccaaagaa
     3961 gtcagtccag tatttgtcca aaaatggcaa aaagaagctg agaagctaga gttcgccctc
     4021 ggacaaatcc cagaaaaaaa tctggaagaa cgtcaagttc tagtagataa gattcaagct
     4081 atcaaggagg tgctccatgt tagcaagtga agtgatccaa gcttatgaag ccttttgccc
     4141 tcaggaattt tctatggagg gagacagctg tggtctgcaa attggcactt tagacaaggg
     4201 tatccaaagg gtcatggtgg ctctcgatat tcgtgaagag acggtggctg aagccattga
     4261 aaagggtgta gacttgatta tcgtcaagca cgcgcctatc tttcgtccta tcaaggactt
     4321 gcttgccagc cgtccgcaaa atcagattta catcgacctg attaagcatg acatcgcagt
     4381 ttatgtcagc cataccaata ttgatatcgt tgaaaatggg ctcaatgact ggttttgtca
     4441 gatgctagga atcgaggaga cgacttatct gcaggaaaca ggtccagaac gtggaattgg
     4501 acgtattggg aatattcagc ctcagacatt ttgggaattg gcccaacagg tcaagcaagt
     4561 ctttgaccta gatagccttc gaatggtgca ttatcaagag gatgatttgc agaagcctat
     4621 ttcaagagta gcaatttgtg gtggaagcgg gcagtctttc tataaggatg ctttggcaaa
     4681 gggggcagat gtctatatca ctggtgatat ctactaccat actgctcagg atatgctgtc
     4741 tgatggcttg ttagcattgg atccaggtca ctatatcgaa gtgctttttg tggaaaaaat
     4801 cgcaacactc cttactcaat ggaaagagaa aaaaggctgg gaactagaga ttttacctag
     4861 tcaagcatcg accaatcctt tccgtcatat ctagttagaa agtgaagaca atgaaaaaag
     4921 ttgccattat tggagcagga attgtgggag caacagctgc ctactacctc tcgagagaaa
     4981 gtgacctaga ggtgaccgtt tttgaccatg agcaaggtca agccaccaag gccgcagcag
     5041 gaattatcag tccttggttt tccaaacgcc gtaataaagc ctggtacaag atggcgcgct
     5101 tgggggctga tttttatgtg gatttattag ctgatttaga gaaatcagga caagaaatcg
     5161 acttttacca gcgttcggga gtctttctct tgaaaaagga tgaatccaat ttggaagaac
     5221 tttatcaact ggccctccag cgcagagaag aatctccctt gatagggcaa ttagccattc
     5281 tgaaccaagc ctcagctaat gaattattcc ctggtttgca gggatttgac cgcctgctct
     5341 atgcttctgg tggagcgaga gtagatggtc acctcttagt aactcgtttg ctggaagcca
     5401 gtcaggtcaa gttggtcaaa gaaaaagtga ctctgacact cttatcatca ggctaccaga
     5461 ttggcgaaga ggtgtttgat caggttattt tggcaacggg agcttggttg ggggacatgt
     5521 tagagccttt aggttatgaa gtggatgtcc gtcctcaaaa aggacaacta cgagattatc
     5581 agcttgccca agacatggaa gattaccctg ttgtcatgcc agaaggggag tgggatttga
     5641 ttccctttgc aggtgggaaa ttatccttgg gcgctaccca tgaaaatgac atgggatttg
     5701 atttgacggt agatgaaccc ttgctccaac aaatggagga ggcggccttg cctcattacc
     5761 cagttttagc tagagtgact tcaagggctg agcgtgtggg aatccgtgcc tataccagtg
     5821 atttctctcc cttctttggg caggtgccag aattggcagg tgtctatgca gccagtggac
     5881 taggttcatc aggcctcaca actggtccta tcattggtta ccatctagcc caactgatcc
     5941 aagacaagga gttgaccttg gaccctgaaa attacccaat tgaaaactat gtcaaacgag
     6001 taaaaagcga ataatactca atgaaaatca aagaacaaac taggaaacta gccgcgggtt
     6061 actcaaagca ctgctttgag gttgtggata gaactgacga agtcagctca aaacactgtt
     6121 ttgaggttgt ggatagaact gacgaagtca gtaaccatac ccatggcaag gcgacgttga
     6181 cgtggtttga attggatttt cgaagagtat aagcatttta ctgaaatttt atcaggtagt
     6241 ttaggatggc aaatgacatt ccctgtcaaa aatggtaaaa taagaaaaaa tgatctgaga
     6301 atcgaggaaa aaagatgcaa gaaaagattt tggtaactgg tggtgccggt tttattggaa
     6361 cccacactgt tattgaattg atccaagcag gccatcaagt tgttgtggtg gataatcttg
     6421 tcaatagcaa tcgtaagagt ttagaagttg ttgaaagaat cacaggagtt gagattcctt
     6481 tctatgaggc agatattcgt gacacagata ccctcagaga tattttcaag caggaagaac
     6541 cgactggtgt cattcacttt gctggtttga aggctgttgg cgaatcaaca cgtatccctc
     6601 ttgcctacta tgacaacaat atcgctggaa ctgtcagcct tttgaaagcc atggaagaaa
     6661 acaactgtaa aaacatcatc ttcagttctt ctgcgacagt ttacggagat ccgcacacag
     6721 tgcccatctt ggaagatttc ccactttcag tgacaaaccc atacggtcgt accaagctca
     6781 tgctagagga aattttgacc gatatttaca aagcagactc agaatggaat gttgtcttgc
     6841 ttcgttactt taacccaatc ggagcccatg agagtggtga tttgggagaa aatccaaacg
     6901 gtattccaaa caatctcttg ccatatgtga ctcaagtagc cgttggaaaa ttagagcaag
     6961 tgcaagtgtt tggagacgat tacgatacgg aagatggaac aggtgttcgt gactatatcc
     7021 acgttgtcga tttggctaag ggtcacgttg cagctttgaa aaaaatccaa aaaggttcag
     7081 gactaaacgt ttataacctt ggaactggta aaggttactc agttcttgaa attatccaaa
     7141 acatggaaaa agcggtggga cgtcctattc cttaccgcat cgtagaacgt cgcccaggtg
     7201 atatcgctgc ctgctactca gacccagcaa aagctaaagc agaactcggt tgggaagcag
     7261 aactcgacat cacccaaatg tgtgaagacg catggcgttg gcaaagcaag catccaaatg
     7321 gatttgaaga ctaagatgat gatttcaatc atcgtccctt gtttaaacga agaggaagta
     7381 cttcctcttt tttatcaggc tctggaagct ttacttccag atttggaaac agaaatcgag
     7441 tatgtctttg tcgatgatgg atcaagtgat gggaccttgg aactcttaaa ggcctatcgg
     7501 gagcaaaatc cggcagtcca ttatatttct ttctctcgaa attttggcaa agaagcagcc
     7561 ctttatgcag gcttgcaata tgcgacagga gatttggtgg tggtgatgga tgcagacctc
     7621 caagatcctc ctagtatgtt gtttgagatg aaaaatgtac tagacaaaaa tgtagacttg
     7681 gactgcgttg ggacacggag aactagtcgg gagggagaac ccttctttcg cagtttctgt
     7741 gctgttctct tttatcgcct catgcaaaaa atcagcccag tagctctgcc gtcgggtgtc
     7801 cgtgattttc gtatgatgag aaggtctgta gtcgatgcca ttttaagctt gaccgagtcc
     7861 aatcgttttt caaaggggct cttcgcctgg gtgggtttca aaacctacta tttggactat
     7921 ccaaatgtcg aaaggcaggc tggcaagaca agttggagtt ttaggcaact ctttttctac
     7981 tccattgaag ggattattaa tttttcagat tttcccttga ttatagcctt tgtagctggt
     8041 ctcctatctt gttttctttc tctgctgatg accttttttg ttgtggttcg gaccctcatt
     8101 ttgggcaatc cgacatctgg ttggacctct ctgatggctg ttattctctt tcttggaggc
     8161 attcaactct tgaccattgg gattctcggc aagtatatca gtaagattta tttagagact
     8221 aaaaaaagac cactttatct tatcaaagaa aaaagtgacc ttcctgattt tacagaaaaa
     8281 aataaagtga aaagactata attttacatg gaaatgtgct aaactagagg gagtggatta
     8341 tcgcccttga tttaggcttc ctggtttcca atcagggcgc gagttgggca atttttaaca
     8401 gcctctaaga catcctgact aggagaaatt tctttttcca gttggtcagg gtcatcgtaa
     8461 aaacgcacga ttccattatc gtggtaatca aataaatcag aataagtttg gcaaagccca
     8521 caggcgatac atcgttcagg tataagtgtg attttcatgt tcatatttat attgtaataa
     8581 gaaaagtaaa aaaaaacaag gagtaaggta tggcaaaaga accgtggcaa gaagatatct
     8641 atgatcaaga agaatcaaga gcagagcgtc ggcatcgaaa ccacggaggg gctgatagga
     8701 tggctaatcg tattttgacg atcctagcta gtattttctt tgtaattgtg gtggtgatgg
     8761 tcatcgttct catctatcta tcatcggggg gaagtaatcg cacagcagcc ttaaaagact
     8821 ttcatgattc tgatgcaagt gtagtacaaa tctcatcttc aagcagttct catcctgagc
     8881 agagttcaga gccagaatct acttctagta gttcagaaga agctgctaat cctgaaggaa
     8941 cgattaaagt tctcgcagga gaaggggaag cagctattgc cgctcgtgca ggaatctcca
     9001 ttgctcagtt agaggccttg aatcctgggc acatggctac aggatcttgg tttgctaatc
     9061 caggtgatgt tataaaaata aaataggagc cgatatgaaa acaattcaaa ttgctattga
     9121 tggtcctgct tccagcggta agagtacggt cgcaaagatt attgctaagg attttggatt
     9181 cacctacctt gatacaggag ctatgtatcg tgcagcgacc tatatggctc ttaagaacca
     9241 attaggagtt gaagaagtcg aagcccttct agccttgttg gaccagcatc caatcagctt
     9301 tggacgttca gaaactggag accagcttgt ttttgtagga gatgtggata ttacccatcc
     9361 tatccgtgaa aatgaagtga ccaatcatgt ttctgctatt gcagcaattc ctgaagtgcg
     9421 tgagaaactg gtttctctcc aacaagaaat tgcccagcaa ggcgggattg tcatggatgg
     9481 tcgcgatatt ggaactgttg tattgccaca agcagaattg aaaattttcc tagtagcttc
     9541 tgttgatgag agagcagagc gtcgttacaa ggaaaatatt gccaagggaa ttgaaacaga
     9601 ccttgaaacc ctaaaaaagg aaattgctgc gcgtgactac aaggatagtc atcgtgagac
     9661 ttctcctctc aaacaagcag aggatgctgt ctaccttgat acaactggtt tgaacattca
     9721 agaagtagtt gaaaaaatca aagcagaagc tgaaaaaaga atgtaaatgt agaaaagccg
     9781 atagattgat atcggttttt ttctaaattt gtcaaaatgc tgattttagg gtataatagt
     9841 tgctgttcga gaaattttga ttttcaaaga atagtgaatg atgataagga gaaaccatga
     9901 acaatttacc aaattgtcca aaatgtaact cagagtatgt ctacgaagac ggtgccctac
     9961 tggtttgccc agagtgtgct catgagtgga atcctgctga agttgcagaa gtagaagaag
    10021 gtcttgtcgc tatcgatgcc aacggaaata aattgactga tggtgataca gtaactctca
    10081 tcaaggactt gaaagtaaaa ggtgcgccaa aagatttgaa acaagggacg cgcgtgaaaa
    10141 atatccgcat cgtagaaggc gatcacaata tcgactgtaa gattgatggt tttggtgcca
    10201 tgaaacttaa atcagagttt gtgaggaaga tttaagcatg tcaaaacact ataaacttgt
    10261 attttatagc cgtatcttct tgtttctagc ggcttttacg ggagtttatc ttgaaattac
    10321 taagcatggt ggttttggga tgcttctcta ttacacggtt ctgtccaacc tcttggtaac
    10381 tatttttacc ctttatcttc taaaggttat gagccgtgta ggtgaaaatt ggcaaaggcc
    10441 aagtctcttg cgcttaaaag gtggggtcac catgagtatc atgattacct gtgtgattta
    10501 ccatttcctc ttggcgccca ttgcgactaa tttctatacc ctagaaaatt tcctttgcca
    10561 ctatatcgtt cccatctggt ttttagcgga taccctcttt tttgacaaac agggtcaata
    10621 caagatttgg gatccagcag tgtggaccat tttacccttt ctgtatatga tgtttgctct
    10681 ttttaatggc ttggttctaa aactcaatat tccaaatgcc aaggataatc ctttccctta
    10741 cttctttttg aatgtgaaca agggttggaa tgttgtgttt aagtggtgtc tgattatctt
    10801 tgttgcctat atggtagcag gatttatttt ctactttatc aagcaaatca agagaaagtc
    10861 atcctaatac tcttcgaaaa tctcttcaaa ccacgttagt ttccatctgc aacctcaaaa
    10921 cactgttttg aacaacctgc ggctagcttt ctagtttgct ctttgatttt cattgagtat
    10981 gagatacaag ggattcattt tcgaatttaa gaaggagtct acaaaccaat gaggcttctt
    11041 cttttcttgc ttatttgata actatcaaaa aagtagaaga ttaagagaaa tatagaaaga
    11101 gatttcatga aacaattttt agaacgggcc agcattttgg ctctctccct cgttttgatt
    11161 acctcctttt ccatttcgag tgccctacca gccatgtttg actattatca gggttattct
    11221 aaggaacaaa ttgagctctt ggtgagcttg ccttcctttg gaatcatgat gatgttactg
    11281 ctaaatggtt tcttagaaaa aatatttcct gagcgcttac agattagttt gggcttgctg
    11341 attttatcat tgagcggtac agctcccttc tggtaccaag cctatccctt tgtctttgga
    11401 acacggcttc tctttggttt gggtcttggg atgatcaatg ccaaggccat ttctattatc
    11461 agtgaacgct accaaggaaa aaggcgaatt cagatgttag ggctacgcgc ttctgcagag
    11521 gtcgttggag cttctctcat taccttggcc gtcggtcagt tgttggcctt tggttggaca
    11581 gctatctttc tagcctatag tgctggattt ttggtgatgc ccctttatct gctctttgtc
    11641 ccttatggaa aatcaaagaa agaagtcaag aaaagagcga aggaagcaag tcgtttaact
    11701 cgagaaatga aaggcttgat ttttacctta gctatcgaag cggcagttgt agtttgtacc
    11761 aatacagcta ttaccatccg tattccaagt ttgatggtgg aaagaggatt gggggatgcc
    11821 cagttatcta gttttgttct tagtatcatg cagttgatcg ggattgtggc tggggtgagt
    11881 ttttctttct tgatttctat ctttaaagag aaactgctcc tctggtctgg tattaccttt
    11941 ggcttggggc aaatcgtgat tgccttgtct tcatccttgt gggtggtagt agcaggaagt
    12001 gttctggctg gatttgccta tagtgtagtc ttgacgacgg tctttcaact tgtctctgaa
    12061 cgaattccag ctaaactcct caatcaagca acttcatttg ctgtattagg ctgtagtttc
    12121 ggagccttta cgaccccatt tgttctaggt gcaattggct tactaactca caatgggatg
    12181 ttggtcttta gtatcttagg aggttggttg attgtaatct ctatctttgt catgtaccta
    12241 cttcagaaga gagcttagga ttgattccta agtttttctt ttgatactca atgaaaatca
    12301 aagagcatag ttgattgagt ttggaatagt acgctgtagc tgctaaaata tttctagaaa
    12361 ttaatttgac ttttctaata gagttattca tatcttattt caatctacta tagaaagcta
    12421 gccgcaggtt gctcaaaata gtgtgttgag gttgcagatg gaaactgacg tggtttgaag
    12481 agattttcga agagtatgag atttttatac ccatacaata tttgttttag gtccttgttt
    12541 acagactaat ttcttgataa aatagaggtt atgacgagat ataaagcaac tatttcctat
    12601 gatggttatg cctttgctgg ctttcagcgc cagtctcatg cgcgtagcgt tcaggaagaa
    12661 attgaaaaaa ccttgaccag attaaataaa gggcaaacca ttactgttca cggtgctggt
    12721 aggacagata gtggggttca tgccctggga caggtcattc attttgacct gccttatcag
    12781 atggatgagg agaaactccg ttttgccttg gatacccagt ctcctgaaga tattgatgtg
    12841 atttcgattg agcttgtggc agatgatttt cattgccgtt atgccaaaca tagcaagacc
    12901 tatgagttta ttgtggatag aggacgtccc aaaaatccta tgcgccgtca ctatgccacc
    12961 cactttccct acccactcga tgtggaacga atgcagattg caatcaaaaa gctagaggga
    13021 acccatgatt ttaccggttt tacagcctct gggactagtg tagaggataa ggttcgcacc
    13081 atcacagaag ctagtttaat agtcgatgag acaggacaat ttttgacctt taccttttca
    13141 ggaaatggtt tcttgtataa acagattcgc aatatggtgg ggactcttct caaaatcggt
    13201 aacaaccgca tgccagtaga gcagattgat ctcatcttgg agaagaagga caggcaactt
    13261 gcaggtccca ctgcagcacc aaatggtttg tatttaaagg agattcgtta tgaagaataa
    13321 tcgtatttta gcactttctg gaaatgatat ttttagtggt ggtggactgt cagctgattt
    13381 ggctacctat accttgaacg gcttgcatgg gtttgtagca gtgacttgtt tgacagcctt
    13441 gacagaaaaa ggatttgaag tctttccaac tgatgatacc atttttcaac atgaattaga
    13501 tagcttacgt gatgtggaat ttgggggaat taagattggt cttctcccta ctgtcagtgt
    13561 ggctgagaag gccttggact ttatcaaaca acgcccagga gtacctgtgg tgttggatcc
    13621 tgtcttggtc tgcaaggaaa cgcatgatgt agctgtcagt gagctctgcc aagagttgat
    13681 tcgctttttc ccttatgtca gtgtgattac gcctaatctc ccagaagcag aattattatc
    13741 cggtcaggaa attaaaacct tggaagacat gaaaactgca gcgcagaaat tgcatgattt
    13801 aggagcgcca gcagtcatta tcaagggagg caatcgtctt agtcaggaca aggctgtgga
    13861 tgtcttttat gatggacaga cctttactat cctagaaaat ccagttatcc aaggccaaaa
    13921 tgctggtgca ggttgtacct ttgcctctag cattgccagt cacttgatta aaggtgataa
    13981 acttttgcca gcagtagaaa gctctaaggc tttcgtttat cgtgctattg cacaagcaga
    14041 tcagtatgga gtaagacaat atgaagcaaa caaaaacaac taaaatcgcc cttgtatccc
    14101 tattaaccgc cctttctgtg gttctaggtt atttcttaaa aatcccaaca cctacaggaa
    14161 ttctaactct tttagatgct ggtgtcttct ttgcggcctt ttactttggt agtcgtgaag
    14221 gagcggtagt cggaggacta gcaagtttct tgattgacct cttatcaggc taccctcagt
    14281 ggatgttctt tagcttggtc aaccatggct tgcagggatt tttcgcagga tttaaaggaa
    14341 aaagtcagtg gttaggcctt attttagcaa ctattgccat ggtaggaggc tacgccttgg
    14401 gttctacttt gatgaatggc tgggcagcag ccctcccaga aattctacca aatttcatgc
    14461 aaaatatggt agggatgatt gtaggattta ttcttagtca aagtatcaag aagattaagt
    14521 aatactcttc gaaaatctct tcaaaccacg tcagcttcca tctgcaacct caaaacactg
    14581 ttttgagcag cctgaggcta gctttctagt ttgcactttg attttcattg agtataaaga
    14641 ggttgagtaa aaagtatttc aaataagaaa aacgcatagt atcaggtgtt gaatgaacta
    14701 cccccc
//
LOCUS       DAVMEI010000035        14554 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVMEI010000035 DAVMEI010000000
VERSION     DAVMEI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14554
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.035"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1367)
                     /locus_tag="VYQ24_000847"
     CDS             complement(<1..1367)
                     /locus_tag="VYQ24_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEV1968009.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKKGYLSTIPVDRYPKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMEI
                     RLRSKL"
     gene            complement(1390..1542)
                     /locus_tag="VYQ24_000848"
     CDS             complement(1390..1542)
                     /locus_tag="VYQ24_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1968010.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            complement(1545..2930)
                     /locus_tag="VYQ24_000849"
     CDS             complement(1545..2930)
                     /locus_tag="VYQ24_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1968011.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2959..3345)
                     /locus_tag="VYQ24_000850"
     CDS             complement(2959..3345)
                     /locus_tag="VYQ24_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV1968012.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3361..3675)
                     /locus_tag="VYQ24_000851"
     CDS             complement(3361..3675)
                     /locus_tag="VYQ24_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEV1968013.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            4348..4647
                     /locus_tag="VYQ24_000852"
     CDS             4348..4647
                     /locus_tag="VYQ24_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968014.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIVDGKAVEVTLREPYSRDLLKVVKSIKQRQKLIAYRYKEGKLLFVKKEASDVL"
     gene            4637..5125
                     /locus_tag="VYQ24_000853"
     CDS             4637..5125
                     /locus_tag="VYQ24_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968015.1"
                     /translation="MSSEQQERMAVQYAERSLLFTVKSLLKILEWSRRQALAQDSAYK
                     IGVQKLEELLQSPYAIDTINLKKDFLDKPIDIEKFKAFLEKEEIPLAIAWQGDSLHFY
                     AKDRSILDNHLDHLLEKMVNDPEKLADFTMDKSLDDAIDEAKSQITFRQEGAVKQKEM
                     VR"
     gene            5125..7002
                     /locus_tag="VYQ24_000854"
     CDS             5125..7002
                     /locus_tag="VYQ24_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEV1968016.1"
                     /translation="MYSGKKFLLFSLLGILLGYLFHRLTLLYDSYTGNTLDKWTHLLM
                     EGQDEVLQSPWNVSFTGKSSAFFLLGFVMMLLVYLYLETGKKQYREGVEYGSARFGTL
                     KEKKLFYGKEFSHDTILAQDVRLTLLDKKPPQYDRNKNIAVIGGSGSGKTFRFVKPNL
                     IQMNSSNIVVDPKDHLAEKTGKLFLEHGYQVKVLDLVNMKNSDGFNPFRYIETENDLN
                     RMLTVYFNNTKGSGSRSDPFWDEASMTLVRALASYLVDFYNPPKTREQLIEESRLSQK
                     EYQNLLKRQKKEVEERKKRGRYPSFAEISKLIKHLSKGENQEKSVLEILFENYAKKYG
                     TENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNIQSVMDLTKRDTLDMKTWGKEKS
                     MVYLVIPDNDSTFRFLSALFFSTVFQTLTRQADIDFKGQLPLHVRVYLDEFANIGEIP
                     DFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLVYLGGNDEDTFK
                     FMSGLLGKQTIDVRNTSRSFGQTGSGSLSHQKIARDLMTPDEVGNMKRHECLVRIANM
                     PVFKSKKYNSTKHPNWKYLANQESDERWWNYQINPLNQRQENYLDDLRIRDLTFESSL
                     K"
     gene            7023..7265
                     /locus_tag="VYQ24_000855"
     CDS             7023..7265
                     /locus_tag="VYQ24_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968017.1"
                     /translation="MITHFKGFVYGVDASAMFAQAMSLLQKGLIAVGAFLVVVGIVNL
                     ATNIKDGGPGVRNAILEIVGGVMVGAAGAFVTQISI"
     gene            7282..8136
                     /locus_tag="VYQ24_000856"
     CDS             7282..8136
                     /locus_tag="VYQ24_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL"
                     /protein_id="HEV1968018.1"
                     /translation="MNLSLVSPFVYLASEKISAENLFEGFNVDLQSTVDLIKSLSSYN
                     PTVWTYMSSITKSVMQPLGVAILSVVLILEFSKMAKKIANSGGAMTFEALAPMLISYI
                     MVAVVITNTTVIVEAIIGIASHAIEQVASIVAHGGAKYDTLSGLKGSGFIGRMIVGFF
                     ALLIWLVRIVSAAMVNLLVSIRFIQLYLMIPFAPLTIPTFLSDEWKSIGIGYLKNIMV
                     YAVQGVLIFLIVSLVPLFESAGKIAVSNGAGVLQSLAIMFGSLVQAILLIIALVGSQR
                     TARSILGM"
     gene            8190..8549
                     /locus_tag="VYQ24_000857"
     CDS             8190..8549
                     /locus_tag="VYQ24_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEV1968019.1"
                     /translation="MNTCVFKDISKYQHRAWLGFTTRQIIFVLPAFIVTIIVLGLNLF
                     FWQFGDWFVYGFVFAFTIPLMLFGVYKPNDLYFEHYLKYRLHFELTVPLRTITGKKGP
                     EHEKKIKYIKETKNFND"
     gene            8500..10857
                     /locus_tag="VYQ24_000858"
     CDS             8500..10857
                     /locus_tag="VYQ24_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1968020.1"
                     /translation="MKRKSNTLKKQKTSMTNKKEEVKGKKEEVLPSTANTLSYQALYQ
                     NGLMQVKEDYFSQSYLLGDVNYQTVGLEDKGAIIEKYSDLINSLDDQTNFQLTIFNKR
                     LNLEKFRQSVLYEEKEDGYDTYRKELNRMMNQNLDSGENNFSAVKLISFGRKDSNPKQ
                     AYRSLSQIGEYFKSGFSEIDARFESLAGEDRVNLLADMLRGEHHLPFSYRDLTRSGQT
                     TRHFIAPNLLDFKNKNYLQINDRLLQIVYVRDYGMELGDQFIRDLMQGDLELIVSLHA
                     QSSTKADAMKKLRTKKTLMESQKIGEQQKLARTGIYLEKVGHVLESNIDEAEELLKTM
                     TETGDKLFQTVFLIGIFGQDEEELKQALDTIQQVAGSNDLMIDKLPYMQEAAFNSLLP
                     FGCDFLEGVSRSLLTSNVAVNSPWTSVDLQDRSGKYYGINQISSNIITIDRSLLNTPS
                     GLILGTSGAGKGMATKHEIITTKIKESGENTEIIIVDPEAEYSVIGRAFGGEMIDIAP
                     DSQTYLNVLDLSDENMDEDPVKVKSEFLLSFIGKLLDRKMDGREKSIIDRVTRLTYQS
                     FKEPSLEEWVFVLSQQPEEEVQNLALDMELYVEGSLDIFSHKTNIQTGSNFLIYNVKK
                     LGDELKQIALMVVFDQIWNRVVRNQKLGKKTWIYFDEMQLLLLDKYASDFFFKLWSRV
                     RKYGASPTGITQNVETLLLDPNGRRIIANSEFMILLKQAKNDREELVQLLGLSKELEK
                     YLVNPEKGAGLIKAGSVVVPFKNKIPQGTQLFDIMSTDPDKMASN"
     gene            10869..13682
                     /locus_tag="VYQ24_000859"
     CDS             10869..13682
                     /locus_tag="VYQ24_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1968021.1"
                     /translation="MKDKRKIIRARKAFRRSLKDEKKFLKKGKKEVRKQKKDSAVLDE
                     KAWKKEIKEKLEEMREASKERVKQANEDYNHILQNSPPSLLNRKELRDRRLPHARKRL
                     KIAKKQFREAKVEAKEERKESRKERKTNQKFLYGQETKAKSNFFFQGKSLEELKAKKE
                     VKDAKENLKSTKQAYKSKKVSRKAKTFLYVLGREGGELASENEDLEGYRTLQETIRKG
                     KRYSRLSYNLGKASVKTGQATGRFTKKRLTNTKERYHHFKDGKGWKLAKDNPSSFKNR
                     YRKLKKQGLTSVRNIYQKLKAAFSFFTFAAGNPATWIVGGIVFLLLLMMSFFLGFSSA
                     SLIQQDEFELTKAYTHLTWEDAEHTRTNDKGITYYTKVDDVMGYMNFKFHDYELHKPV
                     HLFSSETYKDYLSTLWHDLNDGEDLKSMQDLYETPKYKLSKDDQEEMKELKEEGVYAS
                     MQELDNPFEGKSNEDSLTMTYRYGYYDLDGKPTLQEYILLEAKAHQAIVAPMDGVVSL
                     DGDNVILTNGKGENESRLTLYSIHNGRAIEGTRVLTGDIIGETPDDTGLKVSYQKFKN
                     KKEKLVYVNPQFYFPKVIQLQTTILPAIGQFGGDEFERAKHIYEFLKSQGASPQAIAA
                     ILGNWSVESSINPKRAEGDYLSPPVGATASSWDDETWLAIGGPAIYSGAYPNILHRGL
                     GLGQWTDTADGSTRHTALLNYAHSKNKKWYDLDLQLDFMLHGDSPYYQSWLKDFFKNT
                     GSAANLAQLFLTYWEGNSGDKLLERQTRATEWYYQIEKGFSQTNGGQAKSDPQSLEGV
                     RGDLYDHSVPGGGDGMAYAYGQCTWGVAARMNQLGLKLKGSNGEKISIINTMGNGQDW
                     VSTASSLGGETGSIPRAGAIVSFVGGTHGTPAIYGHVAFVEKVYDDGSFLVSETNYGG
                     NPNYTFRKISQADSAISFAYTVK"
     gene            complement(13717..14325)
                     /locus_tag="VYQ24_000860"
     CDS             complement(13717..14325)
                     /locus_tag="VYQ24_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968022.1"
                     /translation="MTIQVTPLKQYLENIQVLAPDKTEKQVQELFKTIILENVDFNGN
                     EEMLTYLSDKAPNFEKQHRSRNFIVEETETNNIIGFFSLSLKVVDISDLEKSLKKKLV
                     LKGKSPKNIDYLPVLLIGQFGKNTNLNKLSGQELFEIVIQKIEEFRAIVGTQMVFLDS
                     INHPKVIQFYEQFGFVAYSQLIKDDHQVTYQPMALNMSLYKK"
     gene            complement(14327..14491)
                     /locus_tag="VYQ24_000861"
     CDS             complement(14327..14491)
                     /locus_tag="VYQ24_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968023.1"
                     /translation="MATISLTRDIKLTNEDALKILNHKPSKKLQAVLKSIESQDATAP
                     TKNKLISKYL"
BASE COUNT         4558 a         2598 c         2893 g         4505 t
ORIGIN      
        1 agtttgcttc taagtcttat ttccataact tcttttacgt ttccgccatt ctttgctgtt
       61 tcgattttta tgatatggtg caagtcagca cgaacacgaa ccgtcttatc tcccattata
      121 tctttttttg gatatctgtc aactggtatg gttgacagat atcctttttt tgttgttatc
      181 acgtcctgtt cttttactga ctgttgcttc aaaatctgct tgtgtcggtc tgtcagtttc
      241 gcatggtcga gaatgtcttt tacaacctgc gtctggttga tttcatcaag tttaatcgca
      301 acctttaagg tcggggcaac ttgatgagat agccagttca gcgtcctttg gaaggagtaa
      361 ggctctggtt ttgtggttag ttttaatcgt tcacgattgt tcccaataaa ccaagcccat
      421 tcttcattca gtttccaatc agaacgaggt ttggaatcgt ctttatctac aaaacggata
      481 taccgattga taattttaaa ggcggtatgc tctggattgt catagacgag taaatcacgg
      541 actgcataat aggcacgctc atttttcaat cgaatctcaa aacggttttt tacttctgcg
      601 tcttcaatgg gaatatcatt tttcttgtac tgctcgtagt ccttttcata gatacagaaa
      661 taaacttcac tttgtaatga accgatatag agggtgtttc ccatacattc cttttcctct
      721 ttgcgtacca gttcgccact gcgatagctt ttaaaactgc ggaagacgga gatacattct
      781 tcctgttggc acttttcagt gagtacaggg atattcaaaa tccctgtctt atcgttaatg
      841 gcaaggtcaa ggcgtttcat cacaccgcca gccaccaaaa cgtccataaa gaactcatac
      901 cagcttcttt gttgtgccag aagatagctt tcaaattgtc tgcacccacg acctttcaat
      961 tccaccagaa ctcctttgtc cagttcatgg gagcaaagga cgaatatgtc gcctaaagca
     1021 taatgctctg aataagaata gaaaccatag tcctcatgaa gaaaatagga cagtttcagt
     1081 tgtaagatgt tttcgaccac ctgctgtacg tctgttgtcg gaaagcgaat ccttacataa
     1141 tcaaacagca tttcaagggg agcgtcggga ttgaagcgtt ccagagcttc ccaaagggac
     1201 tgctgtaaat cctctgatgg cttgactttt cctgtttcaa tatcgcttag atactgcctt
     1261 gtaataccag tcgcaacagc taaacggttt tgagatagtc cataagccaa gcgtttttct
     1321 tttaaatgct gtaaccaagt ttgttcattc agtaaaaatc cctccaatca aaaaggcgta
     1381 tgtcaacttt taaagcccat ttgacatacg ctgaaatttt gtaaatccct tgtaaccaaa
     1441 ggattttcta atgttttttt gactgtttcc tgtcgatttg tacccccctg ttagatacgg
     1501 ggggttaagt gctggcgtgg ctattgccac accagccagc aagatcagtc cacacctgcg
     1561 acttccgctt cgcacgtcgc ctgcgtggac tgtctgctgt tggataactt tttaatttcc
     1621 tccaagaaat catatccttt tggtacaagg ggagtataaa actctgatat gacacttgtt
     1681 cctacatcaa catagccacg acctttgatt cgctttaaga agaaatcctt ttgtacgtca
     1741 ctgccaaaca tcatgccata gcccatttca gacatacgac ctaaagccac tctgaaatta
     1801 aactgatcac ggattccgtc gcctaaatat tttgcgtctg gacgttgaca agccagtatt
     1861 agaaagaagc cagcttgacg acctaacatg acaatctgtt tcagcttatt cataactgcg
     1921 gtgttttctt ttgttcccag catttccatg aaagcgacgt attcatcaaa gattaagaag
     1981 tgtgccggga gacctaagta agcataattt ttgccagtct tatagttctt catctgcttc
     2041 atttcctcac tacgtttcat catttcttca tagaatgttt caatgcaaga aagcaagtct
     2101 tcttttctat agtagacatt tgccatcaca gaacctaagt ccgcaaggtc agcatttttc
     2161 gggtcaagaa tatacagttt tgaatctgta tgaagcaagg cttcaatcag tgtcagtata
     2221 aagtaagttt taccgccacc tgtaccacca gcaatcaaca tatgagggag cttatcatat
     2281 tcccaccata cgtttttcat taagcgaagt ttaccatctt tagcttctac ttcatcaata
     2341 gaaatacgac tggctatggt gtcatagagc aaagtatatt ccacatagga atcctttaac
     2401 tctttatccg tcagctcaca gtacaagcca ctctctaatt tcttttccaa gtgtaagagt
     2461 tggtcttgat attttcccag cgtgatttcc acccgtatct gtatcaagcc atttttaagt
     2521 cgataataca ttttagggaa gtaggttatc ttttcctttg tacgaccagc actatcttta
     2581 aagaaaccct ctgttttgac ctgttcagat tcataccact tgttttcaag tatcatcttt
     2641 gccagttttt gacggtggta aagttgttta accgtatcat agcgaacccg tttgaataca
     2701 aacgctacca gcaagcagat aagaattgcg acactgaaac tgataattaa atagggaatg
     2761 tcaatcttat ctgcttgtga taggttaaaa tcctgccagt tgatctgctg gattgtcttc
     2821 acatgaaaca gtccgacaac cagcaggaaa acaggcagga gtgacgctat cgtaaaatga
     2881 aagactaaat ctttaccaga tgggcgaatc cttttaccac gctgtttcat gcgaaaaagt
     2941 ctcctttcta cctagcgact atttgtcttg tgtcggttct ttctttgctt gtggttgagc
     3001 tttgaatgaa ctagaatcct ttgtcagcac aatatcgtct gccttgatat accagtcaac
     3061 atctgctcct tgataggtgg cagtagcaac ggtgtccgca atgggattga taagttccac
     3121 ccgtgcgtta taatcaaact ctttcaaagg cacgctggca ggaatactta cttgaatcat
     3181 gcgtccttgt cctttggatt ttaagtcata ggtacgttcc ttgatttcat ctgaaaccga
     3241 cccgtcttca ttttggattc tcacttcacg acgtagagca gagaatttca attctccaaa
     3301 agtcgtgtct ttatctaata caatgccatt tgctaatctc atcatttttc ctctctttct
     3361 ttattctttt atcatgtcgt cagcatgtaa aaggtaattt gtaaaaccac gagtgccgat
     3421 tttgtagccc tctgcggtaa tacgtggatt gactaacttc acacgttcct caaagccgaa
     3481 atgtttttcg ccagcttcag caggaagcac caccacaata tcatctgctc tttgaacatc
     3541 agaatagaga ttatagcttc ttgataagac agttagccgt ccgttgattc ttcgctgaac
     3601 gactttatcc tcgccagcaa attctaaatt gccgaatgtt ttttccatgt tgggaatcac
     3661 aaatttaagt tccatatttt tacctatcct ttctttttta ttggctgaat gaatgtttga
     3721 tggtcttaaa gagtggggaa cgaccttttg attcttgatt ttttgttttc ataagttcac
     3781 ttcctttcaa aatcgggtaa aaaaatagac acctcatttt ttgaagtgtc tacctattaa
     3841 atattcaaat tttattggaa gtatctttat atcttcactt ttcaaggata aatcgtcgta
     3901 tcaaagctca ttcataagta gtaaattagt agtaaattga gtggttttga ccttgataaa
     3961 gtgtgataag tccagttttt atgcggataa ctagattttt atgctatttt ttagacaaaa
     4021 ttagcttatt ttagggaaat gaaatgctaa cgttatgtaa caatgttagt cttttatttc
     4081 aaaatctttg tttagtatga atttagctct acagaaaaat aaaaaatgtg ataaaaattt
     4141 caactactct tctctataat aaagctacct gttctttgaa aattgaatcc actccgtctt
     4201 gattagcgtg cctgtgtaag tagggactga gagtatttcc ctgtgaaggg caactggcac
     4261 aagacgtgtg tgagaatttt ctaacagaca cggagtttat cgttaccaga cttaaacgat
     4321 gcgacaaact agataaagga gttaatcatg aaggtagtaa acttgtatga tttgaaacaa
     4381 atgggaaata aaggtggttg taccatccaa ttgattcatc actttccttt tggtatgggc
     4441 ttaggacatc tcaaaaaaga ctacattgaa tttaaacgtg ttggtatcgt tgatgggaag
     4501 gcagtagaag tcactcttcg agaaccttat tcgagagatc tactgaaggt tgtcaagtcg
     4561 attaagcaac gtcagaaatt gatagcttat cgttataaag aaggaaagct gctatttgtg
     4621 aagaaggagg catcagatgt cctctgaaca acaggaacga atggcagttc aatatgcaga
     4681 gcgtagtctt ttatttactg taaaaagtct tttaaagatt ctagaatggt ctagacgtca
     4741 ggctttagca caggattccg cctataagat aggagtgcag aaattagaag aattgctaca
     4801 atctccctat gcgattgata cgattaatct gaaaaaagat tttttagaca aaccaattga
     4861 tatagagaaa tttaaagctt ttttagaaaa agaagagatt cctttagcca tcgcttggca
     4921 aggggattct ctacatttct acgcgaaaga ccgttcgatt ttagacaatc atttagacca
     4981 tcttttagaa aaaatggtta atgatccgga gaaattagct gattttacca tggataagtc
     5041 attagatgat gctattgatg aagctaaatc ccaaattacc tttagacaag aaggagccgt
     5101 caaacagaaa gagatggtga gatgatgtac agtggaaaaa aattcctact attctcactg
     5161 ttaggtatct tactaggcta tctttttcat cgtttgacgc ttttgtatga ttcctatact
     5221 ggaaatacct tagataaatg gactcatctt ctgatggaag gtcaagatga agttcttcag
     5281 tcgccatgga atgtttcttt tactggaaaa tcaagtgctt tttttctact aggttttgtg
     5341 atgatgttac tggtttatct ctatctagag actggtaaaa aacaataccg agaaggggta
     5401 gaatacggga gcgcccgttt tggaactcta aaagaaaaga agctctttta cggtaaggaa
     5461 ttttctcatg atacgatctt agcacaagat gttcgtttga cattattaga taaaaaacca
     5521 ccccaatatg atagaaataa gaatattgcg gtgattggag gttcaggaag tgggaagaca
     5581 tttcgctttg tgaaacccaa tctgattcag atgaatagtt ctaatattgt agtggatcct
     5641 aaagatcact tggccgagaa aacaggcaaa ctctttttag aacatggcta ccaagtaaag
     5701 gtgttagatt tagtcaatat gaagaactca gatggcttta atccttttcg ctatatagaa
     5761 acagaaaacg atttgaatcg catgctgact gtttatttta ataacaccaa aggctctggc
     5821 tcccgaagtg atccattttg ggatgaagct tctatgactt tggttcgagc tttagcctct
     5881 tacttagtcg atttctataa tccacctaaa acaagagaac agctcataga agaaagtcgt
     5941 ttaagtcaaa aggaatatca aaacttgttg aaacgtcaaa aaaaagaagt ggaagagcga
     6001 aaaaaacgag gtcgttatcc aagttttgct gaaatctcaa aactcattaa acacttatcc
     6061 aagggtgaaa accaggaaaa aagtgtctta gaaattctat ttgaaaatta tgctaaaaag
     6121 tatgggactg aaaattttac catgcgaaat tgggcagatt ttcaaaatta taaggataag
     6181 actctggatt ctgttatagc tgtaaccact gctaaatttg ccctcttcaa tattcaaagt
     6241 gtcatggact tgaccaaaag agataccctt gatatgaaga catggggcaa ggaaaaatca
     6301 atggtttact tagttatccc agataacgat agtacctttc gctttctttc agccctcttt
     6361 ttttcaaccg tatttcaaac cctaacaaga caagcagata ttgattttaa gggtcaattg
     6421 cctcttcatg tgagagttta cttagatgag ttcgcaaata tcggagaaat tccagatttt
     6481 gctgaacaaa cctcaacagt ccgttctcga aatatgagtc tcgttcctat tcttcaaaat
     6541 attgcccaac ttcaagggct ctataaagaa aaagaagctt ggaaaaccat tttggggaac
     6601 tgtgatagct tagtctactt aggtggtaat gatgaagata cctttaaatt tatgagtggc
     6661 ttactcggta aacaaaccat tgatgttcga aatactagtc gttcctttgg ccaaacaggt
     6721 tcaggatccc tttctcatca aaagattgct cgtgacttaa tgacacccga tgaggtcgga
     6781 aatatgaaac ggcatgaatg cttggttcga attgccaata tgcctgtctt taaaagcaaa
     6841 aaatacaatt ccactaagca tccaaactgg aagtacctag ctaatcaaga aagcgatgaa
     6901 cggtggtgga actatcaaat caatcctttg aatcaaagac aagaaaatta tcttgacgac
     6961 cttagaattc gtgatttaac ttttgaatct agtttaaaat aaaaatagaa aagaggaaat
     7021 agatgattac gcattttaaa ggttttgttt atggagtaga cgcaagtgct atgtttgcac
     7081 aagctatgtc tttgttacag aagggattga ttgcagttgg tgcctttctc gttgttgtgg
     7141 ggattgtcaa ccttgcaacc aacattaaag atggtggacc aggtgttcgg aatgccattc
     7201 ttgaaattgt cggtggtgtt atggtagggg ctgctggagc ctttgtaacc cagatttcaa
     7261 tttaggagga taaacaatga catgaatctt agtttagtct caccctttgt ttaccttgca
     7321 tctgaaaaaa tatcagctga aaatttattt gaaggattta atgtggattt acaatctacg
     7381 gtagatctga ttaaatccct atctagctac aatccaacag tttggactta tatgtctagt
     7441 attactaaaa gtgtcatgca gcctcttgga gttgcgattt tatcagttgt tctcatctta
     7501 gaattttcga agatggcaaa gaagattgct aactctggtg gagcgatgac ctttgaagca
     7561 ttagcgccga tgttgattag ttatattatg gtcgcagtgg tcattaccaa tactaccgtt
     7621 atagtagaag ctatcatcgg gattgcgagt cacgccatcg aacaagtggc ctcgattgtg
     7681 gctcacggtg gggcgaagta tgatacactc tctggattaa aaggttcagg atttattggc
     7741 cggatgattg tgggcttttt cgctctcctt atctggcttg ttcggatagt aagtgcagcc
     7801 atggttaatc ttttggtatc tattcgattt attcaactct accttatgat cccatttgcc
     7861 cctcttacga ttccaacatt tttaagtgat gaatggaagt ctattggtat tggctattta
     7921 aaaaatatta tggtctatgc ggtacaaggg gttctcattt ttctgattgt ttctcttgtt
     7981 cctttgtttg aatctgctgg gaaaatagct gtttcaaatg gtgcaggagt cttgcaatca
     8041 cttgcgatta tgtttggtag tttagtacaa gctatcttac tgattattgc cctcgttggt
     8101 tctcaacgta cggctcgctc aatcttaggt atgtaattag ataaaggcta ggaagtgatt
     8161 gcttcttagc ctttttagaa aggaaagtca tgaatacatg tgtctttaaa gacatctcaa
     8221 aataccaaca cagggcttgg ttaggtttca ccacaagaca aatcatcttt gttttaccag
     8281 cctttattgt cacaattatt gttttgggct tgaatctctt tttctggcaa tttggagatt
     8341 ggtttgttta cggttttgtg tttgctttta ccatccccct aatgcttttt ggagtctata
     8401 aacccaatga tttatatttt gaacattatt tgaaataccg tcttcatttt gaattaacgg
     8461 ttcccctacg cacaattaca ggaaagaaag gacctgaaca tgaaaagaaa atcaaataca
     8521 ttaaagaaac aaaaaacttc aatgactaat aaaaaggaag aagttaaagg gaaaaaagag
     8581 gaagtgttac cctcaacggc taatactctt tcctatcaag ccctgtatca aaatggtctg
     8641 atgcaggtaa aagaagatta tttctcacaa agctatttac ttggtgatgt caattaccag
     8701 accgttggtt tagaagataa gggcgcaatc attgagaagt attctgattt gattaactca
     8761 ctagatgacc aaaccaactt ccaattgact atctttaata aaagattgaa tttagaaaag
     8821 tttagacaaa gtgttttgta tgaggaaaaa gaagatggat atgataccta tcgtaaagaa
     8881 ttgaatcgga tgatgaatca gaatttagat agtggtgaaa ataacttttc ggctgtaaaa
     8941 ctgattagct ttggaagaaa ggattctaat cccaaacaag cctatcgttc cttgtctcaa
     9001 ataggagaat atttcaagag tggtttctca gaaattgatg ctcgatttga atccttggct
     9061 ggagaagatc gtgtgaactt gttggccgac atgcttagag gagaacacca tcttcctttt
     9121 tcttaccgtg atttaacgag atctggtcag acaactcgtc acttcatagc ccctaatctc
     9181 ttggatttta aaaacaagaa ttacctacaa atcaatgacc gcttattaca gattgtctat
     9241 gtcagagact acggcatgga attaggggat cagtttattc gagaccttat gcaaggagat
     9301 ttggaattga ttgtaagcct tcatgctcaa agttcgacca aggcagatgc catgaagaaa
     9361 ctacgaacaa agaagacctt gatggaatcc caaaagattg gggaacaaca aaaactagct
     9421 cgtacaggta tctatttgga aaaagtaggt catgtattag aaagtaatat cgatgaagct
     9481 gaagaacttt taaaaaccat gaccgagaca ggtgataaac tgtttcaaac ggtcttcttg
     9541 attgggatct ttggtcagga tgaagaagaa ctcaaacaag ctctagatac gattcaacaa
     9601 gtggccggct caaatgacct aatgattgat aaacttccat atatgcaaga agcagccttt
     9661 aatagtttgc tgccatttgg ttgtgatttt ttagagggag tatcacggag tttattaaca
     9721 tctaatgtag cagtgaactc accttggact tcagtagact tacaagaccg cagtgggaaa
     9781 tattacggta tcaatcaaat ctcaagcaat attattacta ttgatcgaag cttattaaat
     9841 acaccgtctg gtctgatttt agggacatct ggagctggga aagggatggc aactaagcat
     9901 gagattatca cgaccaaaat caaggaatct ggtgaaaata ctgaaattat catcgtggat
     9961 ccagaagcag aatacagtgt cattggacga gcttttgggg gagaaatgat tgatattgcg
    10021 ccagattccc aaacctatct caatgtactt gacttgtctg atgaaaatat ggatgaggat
    10081 cctgttaagg taaaatcaga atttctttta tcctttatcg gcaagttatt ggataggaaa
    10141 atggatggaa gagaaaaatc gattatcgac cgagtcacca gactcaccta tcagtcattt
    10201 aaagagcctt ctttggaaga atgggtcttt gtcttgagtc aacaaccaga agaagaagtg
    10261 cagaatttgg cacttgatat ggaactgtat gtcgaaggtt ctcttgatat tttttctcat
    10321 aagaccaata ttcagacagg atctaacttc ttaatctata acgttaaaaa gttaggagat
    10381 gagctgaaac aaatcgctct tatggtagtg tttgatcaga tatggaatcg tgttgttcgg
    10441 aaccaaaaat tagggaagaa gacctggatt tattttgatg aaatgcagct tctcttatta
    10501 gataaatatg ccagtgattt cttctttaaa ttgtggagtc gtgtcagaaa atatggagct
    10561 agtccgactg ggataactca aaacgtcgaa accttattgt tagatccaaa tggtagacgg
    10621 attattgcaa atagtgaatt tatgattctc ctcaagcaag caaaaaatga ccgagaagaa
    10681 ctggttcaac tcttaggctt gtcaaaagaa ctcgaaaaat accttgtcaa tccagaaaaa
    10741 ggggcaggac tgataaaagc tggttcagtt gtcgtaccct ttaaaaataa gattcctcaa
    10801 ggtactcaat tgtttgatat catgagtacg gatcctgata aaatggcttc taattaaggg
    10861 gaagtagaat gaaggataaa agaaaaatca tacgtgcccg aaaggcattt agaagaagcc
    10921 taaaagatga gaaaaaattc ttgaaaaaag gaaagaagga ggtaaggaaa cagaaaaaag
    10981 attccgctgt actggatgaa aaagcatgga aaaaagagat aaaagaaaag ctagaggaga
    11041 tgagagaggc ttcaaaggag agagtaaaac aagcaaatga agactacaat catattcttc
    11101 aaaatagtcc accatctctt ttgaatcgca aagaattaag agacagacga ttacctcatg
    11161 ctaggaaacg attgaaaata gctaagaagc aatttagaga agccaaggta gaagcaaaag
    11221 aagaaagaaa agagagtcgt aaagaaagaa aaaccaatca aaaatttctt tatggtcagg
    11281 aaacaaaagc aaaatccaat tttttctttc aagggaagag tttagaagaa ttaaaagcta
    11341 agaaagaagt caaggacgcc aaagaaaatt taaaatctac taaacaagcc tataaatcca
    11401 aaaaggttag taggaaagcc aaaactttcc tttatgtcct tggacgtgaa ggtggagagt
    11461 tagcttcaga aaatgaagat ttagaaggct atcgcacact tcaagagacc attagaaaag
    11521 ggaaacgcta cagtcggctt tcttataatc ttggaaaagc tagtgtcaaa acaggacaag
    11581 caacaggtcg ttttaccaag aaaagactga ccaacacaaa agagcgatac catcatttta
    11641 aggatggaaa aggatggaaa ctagcgaaag ataatccaag ttcctttaaa aatcggtatc
    11701 gaaaattaaa gaaacaaggc cttacaagtg tacgaaatat ctatcaaaaa ctaaaagcag
    11761 ccttttcctt ctttacattt gcggctggga atcctgcaac ctggatagtt ggaggaatag
    11821 tctttcttct tttacttatg atgagcttct ttttaggatt ttcatctgct agtttaattc
    11881 aacaagatga atttgaatta acaaaagctt atacccacct aacttgggaa gacgcagaac
    11941 atacccgaac caatgacaaa ggaattacct attacacaaa agttgatgat gtgatggggt
    12001 atatgaactt taaattccat gactatgagt tacacaaacc agttcactta tttagttcag
    12061 aaacttacaa ggattatcta tctactttgt ggcatgattt aaacgatggg gaagatttga
    12121 aatccatgca agacctttat gaaactccta agtataaact atcgaaagac gatcaagagg
    12181 aaatgaagga actaaaagaa gaaggtgtgt atgcttccat gcaggaattg gacaatccat
    12241 ttgaagggaa aagtaacgaa gatagtttaa ccatgactta tcgttatgga tactatgatt
    12301 tagacggaaa acctactctt caggagtaca ttctactaga agctaaagct caccaagcaa
    12361 ttgtcgcacc aatggatgga gttgtatctc tagatggcga caatgttatt ctcactaacg
    12421 gaaaaggaga gaatgagagt cgattaacct tgtattctat tcataatggc cgtgcgattg
    12481 aggggacaag agtcctaacg ggtgatatta ttggtgaaac accagacgat acaggtttga
    12541 aagtttccta tcaaaagttt aagaacaaga aagaaaaatt ggtgtacgtc aatccgcaat
    12601 tttattttcc aaaagtcatt caacttcaga caactatctt acctgccatt ggtcagtttg
    12661 gtggggatga gtttgaacga gcgaaacata tttatgagtt tttgaaatct caaggggcaa
    12721 gtccccaagc cattgcagct attttaggaa attggtcggt agagtcttct atcaatccta
    12781 aacgagctga gggagattat ttatctcctc cagttggcgc taccgcttcc tcatgggatg
    12841 atgaaacctg gttagcgatt ggaggtccag ccatttatag tggtgcttat cctaatatcc
    12901 ttcatagagg tttggggtta ggtcaatgga cggatactgc agatggttca acacgtcata
    12961 cagctttatt gaattatgca catagcaaaa ataagaagtg gtatgattta gacctccaac
    13021 ttgattttat gcttcatggg gatagtcctt actatcaaag ttggttaaaa gatttcttta
    13081 aaaatacggg cagtgcagcc aatctcgccc aactcttcct cacctattgg gagggaaatt
    13141 ctggtgacaa actactggaa agacaaacca gagcaacgga atggtattac caaattgaaa
    13201 aaggctttag ccaaacaaac ggaggacaag caaagagtga cccacaatcc cttgaagggg
    13261 ttcgtgggga cttgtatgat cattctgttc ctggtggtgg agatggtatg gcctatgcct
    13321 atggacaatg tacatgggga gttgcggctc gtatgaacca gttaggctta aaattaaaag
    13381 gaagtaatgg agaaaagatt tcaatcatta ataccatggg aaatggtcag gattgggttt
    13441 cgacagcttc aagtcttgga ggggaaacgg gctctatacc aagagcaggt gctattgttt
    13501 cttttgtggg aggtacacat ggcacaccag ccatctacgg tcatgtggct tttgtagaga
    13561 aagtctatga tgatggttct ttccttgtgt ctgaaaccaa ctatggtggc aaccctaact
    13621 atacctttag aaaaatctcg caagcagata gtgccatcag ttttgcttat acggtgaaat
    13681 aaaagaatca tgtcatcagt ttaggtgaca tgattcttat tttttataga gtgacatatt
    13741 taatgccata ggctgataag ttacttgatg atcgtcttta atcaattgac tgtaagcaac
    13801 aaatccgaac tgttcataaa attgaatgac tttaggatga ttgatactat ctaaaaatac
    13861 catctgagtt cctacaattg ctcgaaattc ttcaattttt tgaattacaa tttcaaataa
    13921 ctcttgtcct gatagtttat ttagatttgt gtttttgccg aattgaccga taagtaacac
    13981 agggagataa tcaatatttt tgggactttt cccttttagg acgagtttct tttttaagga
    14041 tttctctaaa tcagaaatat caacaacttt gagagataaa ctaaagaatc ctatgatgtt
    14101 atttgtttca gtttcttcaa cgataaaatt acgagaacga tgttgttttt caaaattggg
    14161 ggctttatca gatagataag tcaacatctc ttcattaccg ttgaaatcaa cattttctaa
    14221 aatgatagtt ttaaatagct cctgaacttg tttctcagtt ttatcaggag ctagcacttg
    14281 aatgttttca agatattgct taagtggtgt tacttgaatt gtcatcttac agatactttg
    14341 aaatgagttt atttttcgtt ggagcggtag cgtcctgtga ttcaatagat ttcaagacgg
    14401 cttgtagctt tttagatggt ttatgattaa gaatttttag agcatcttcg tttgttagtt
    14461 ttatatcacg tgttaaactg attgtagcca tttgttctac ctcctgtttc tacttaaatt
    14521 cattatagct tattttgaac aaaagttcaa tcta
//
LOCUS       DAVMEI010000036        14536 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVMEI010000036 DAVMEI010000000
VERSION     DAVMEI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.036"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            13..540
                     /locus_tag="VYQ24_000862"
     CDS             13..540
                     /locus_tag="VYQ24_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1968024.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            619..3360
                     /locus_tag="VYQ24_000863"
     CDS             619..3360
                     /locus_tag="VYQ24_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV1968025.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGIT
                     QVATSYNKANESQTEHRKAAKQVDEDIKKMLSEIQEYIKKMLSEIQLDKRKHTQNVNL
                     NRKLSAIQTKYLYELRVLKEKSKKEELTSKTKKELDAAFEKFKKEPELTKKLAEAKQK
                     AKAQKEEDFRNYPTNTYKTLELEIAEFDVKVKEAELELVKEEAKPRNEEKIKQAKAKV
                     ESKKAEATRLEEIKTERKKAEEEAKRKAEESEKKAAEAKQKVDTKEQGKPKRRAKRGV
                     SGELATPDKKENDAKSSDSSVGEETLPSPSLNMANESQTEHRKDVDEYIKKMLSEIQL
                     DRRKHTQNVNLNIKLSAIKTKYLYELSVLKENSKKEELTSKTKAELTAAFEQFKKDTL
                     KPEKKVAEAEKKVEEAKKKAKDQKEEDRRNYPTNTYKTLELEIAESDVKVKEAELELV
                     KEEANESRNEEKIKQAKEKVESKKAEATRLEKIKTDRKKAEEEAKRKAEESEKKAAEA
                     KQKVDAEEYALEAKIAELEYEVQRLEKELKEIDESDSEDYLKEGLRAPLQSKLDTKKA
                     KLSKLEELSDKIDELDAEIAKLEVQLKDAEGNNNVEAYFKEGLEKTTAEKKAELEKAE
                     ADLKKAVDEPETPAPAPQPAPAPEKPAEKPAPAPEKPAPAPEKPAEKPAPAPAPEKPA
                     PEKPAPTPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQN
                     NGSWYYLNSNGAMATGWLQYNGSWYYLNANGDMATGWFQYNGSWYYLNANGDMATGWF
                     QYNGSWYYLNANGDMATGWLQYNGSWYYLNSNGAMVTGWLQNNGSWYYLNANGSMATD
                     WVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(3590..4570)
                     /gene="dusB"
                     /locus_tag="VYQ24_000864"
     CDS             complement(3590..4570)
                     /gene="dusB"
                     /locus_tag="VYQ24_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1968026.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(4557..5429)
                     /gene="hslO"
                     /locus_tag="VYQ24_000865"
     CDS             complement(4557..5429)
                     /gene="hslO"
                     /locus_tag="VYQ24_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1968027.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            5594..7021
                     /locus_tag="VYQ24_000866"
     CDS             5594..7021
                     /locus_tag="VYQ24_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1968028.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLYSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            7180..8688
                     /gene="glpK"
                     /locus_tag="VYQ24_000867"
     CDS             7180..8688
                     /gene="glpK"
                     /locus_tag="VYQ24_000867"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1968029.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            8730..10556
                     /gene="glpO"
                     /locus_tag="VYQ24_000868"
     CDS             8730..10556
                     /gene="glpO"
                     /locus_tag="VYQ24_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1968030.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKRTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVKAALAQNDLAELKN"
     gene            10624..11328
                     /locus_tag="VYQ24_000869"
     CDS             10624..11328
                     /locus_tag="VYQ24_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1968031.1"
                     /translation="MMSELFGEFLGTLILILLGNGVVAGVVLPKTKSNSAGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEVEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            11576..11722
                     /locus_tag="VYQ24_000870"
     CDS             11576..11722
                     /locus_tag="VYQ24_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968032.1"
                     /translation="MLVEFHKSQGTLETPEVQAEIAQKREEIEQRRAELEGKKQELLN
                     RLNK"
     gene            complement(11809..12417)
                     /locus_tag="VYQ24_000871"
     CDS             complement(11809..12417)
                     /locus_tag="VYQ24_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968033.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAMIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            13505..14536
                     /gene="ltrA"
                     /locus_tag="VYQ24_000872"
                     /pseudo
     CDS             13505..14536
                     /gene="ltrA"
                     /locus_tag="VYQ24_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         4643 a         2697 c         3270 g         3926 t
ORIGIN      
        1 aaggatacta atatggtatt agcgattatt ttagtaacat tccttattcg attgattttt
       61 ttaaagcgtt cgatagagaa tgagaaacga atccttagca atggcgggaa agaatttgga
      121 gttgagaata caaaacgatt aacgttggct catattgttt tttatctctc ttgcctcgtt
      181 gaagcaatgg tgcataagac aatgtttgat ggtatgagca tggttggttt agtcttgctt
      241 attttttcta tgctgatgtt gatgttggtg attcacttgt tgggagatat ttggacagtg
      301 aagcttatgc ttgtcaataa tcacaaatat gtagatcata tcttgtttag gacagtaaaa
      361 catcctaatt actttttaaa tattttacct gagttgattg gcttgacctt gttgagtcat
      421 gcctatatga cttttgtttt agtttttcca gtttatgcag ttattttgta tcgacgaata
      481 gctgaagagg aaaagctatt acatgaagtt ataatcccaa atggaagcat aaagagataa
      541 atacaaaatt cgatttatat acagttcata ttgaagtgat atagtaaggt taaagaaaaa
      601 atatagaagg aaataaacat gtttgcatca aaaagcgaaa gaaaagtaca ttattcaatt
      661 cgtaaattta gtattggagt agctagtgta gtagttgcta gtcttgttat gggaagtgtg
      721 gttcatgcga cggagaatga gggaattacc caagtagcca cttcttataa taaggcaaat
      781 gaaagtcaga cagaacatag gaaagctgct aaacaagtcg atgaagatat aaaaaaaatg
      841 ttgagtgaga tccaagaata tataaaaaaa atgttgagtg agatccaatt agataaaaga
      901 aaacataccc aaaatgtcaa cttaaacaga aagttgagcg caattcaaac gaagtatttg
      961 tatgaattaa gagttttaaa agagaagtcg aaaaaagaag agttgacgtc aaaaacaaaa
     1021 aaagagttag acgcagcttt tgagaagttt aaaaaagaac cagaacttac taaaaaatta
     1081 gcagaagcta aacaaaaagc caaggctcaa aaagaagaag atttccgtaa ctacccaacc
     1141 aatacttaca aaacgcttga acttgaaatt gctgagttcg atgtgaaagt taaagaagcg
     1201 gagcttgaac tagtaaaaga ggaagctaaa ccccgaaacg aggaaaaaat taagcaagca
     1261 aaagcgaaag ttgagagtaa aaaagctgag gctacaaggt tagaagaaat caagacagaa
     1321 cgtaaaaaag cagaagaaga agctaaacga aaagcagaag aatctgagaa aaaagctgct
     1381 gaagccaaac aaaaagtgga tactaaagag caaggtaaac caaagaggcg ggcaaaacga
     1441 ggagtttctg gagagctagc aacacctgat aaaaaagaaa atgatgcgaa gtcttcagat
     1501 tctagcgtag gtgaagaaac tcttccaagc ccatccctta atatggcaaa tgaaagtcag
     1561 acagaacata ggaaagatgt cgatgaatat ataaaaaaaa tgttgagtga gatccaatta
     1621 gatagaagaa aacataccca aaatgtcaac ttaaacataa agttgagcgc aattaaaacg
     1681 aagtatttgt atgaattaag tgttttaaaa gagaactcga aaaaagaaga gttgacgtca
     1741 aaaaccaaag cagagttaac cgcagctttt gagcagttta aaaaagatac attgaaacca
     1801 gaaaaaaagg tagcagaagc tgagaagaag gttgaagaag ctaagaaaaa agccaaggat
     1861 caaaaagaag aagatcgccg taactaccca accaatactt acaaaacgct tgaacttgaa
     1921 attgctgagt ccgatgtgaa agttaaagaa gcggagcttg aactagtaaa agaggaagct
     1981 aacgaatctc gaaacgagga aaaaattaag caagcaaaag agaaagttga gagtaaaaaa
     2041 gctgaggcta caaggttaga aaaaatcaag acagatcgta aaaaagcaga agaagaagct
     2101 aaacgaaaag cagaagaatc tgagaaaaaa gctgctgaag ccaaacaaaa agtggatgct
     2161 gaagaatatg ctcttgaagc taaaatcgct gagttggaat atgaagttca gagactagaa
     2221 aaagagctca aagagattga tgagtctgac tcagaagatt atcttaaaga aggcctccgt
     2281 gctcctcttc aatctaaatt ggataccaaa aaagctaaac tatcaaaact tgaagagttg
     2341 agtgataaga ttgatgagtt agacgctgaa attgcaaaac ttgaagttca acttaaagat
     2401 gctgaaggaa acaataatgt agaagcctac tttaaagaag gtttagagaa aactactgct
     2461 gagaaaaaag ctgaattaga aaaagctgaa gctgacctta agaaagcagt tgatgagcca
     2521 gaaactccag ctccggctcc tcaaccagct ccagctccag aaaaaccagc tgaaaaacca
     2581 gctccagctc cagaaaaacc agctccagct ccagaaaaac cagctgaaaa accagctcca
     2641 gctccagctc cagaaaaacc agctccagaa aaaccagctc caactccaga aactccaaaa
     2701 acaggctgga aacaagaaaa cggtatgtgg tacttctaca atactgatgg ttcaatggca
     2761 acaggctggc tccaaaacaa tggctcatgg tactacctca acagcaatgg cgctatggcg
     2821 acaggatggc tccaaaacaa tggctcatgg tactacctca acagcaatgg cgctatggcg
     2881 acaggatggc tccaatacaa tggttcatgg tactacctca acgctaatgg tgatatggcg
     2941 acaggatggt tccaatacaa tggttcatgg tactacctca acgctaatgg tgatatggcg
     3001 acaggatggt tccaatacaa tggttcatgg tactacctca acgctaatgg tgatatggcg
     3061 acaggatggc tccaatacaa tggttcatgg tactacctaa acagcaatgg tgctatggta
     3121 acaggatggc tccaaaacaa tggctcatgg tactacctaa acgctaacgg ttcaatggca
     3181 acagattggg tgaaagatgg agatacctgg tactatcttg aagcatcagg tgctatgaaa
     3241 gcaagccaat ggttcaaagt atcagataaa tggtactatg tcaatggctc aggtgccctt
     3301 gcagtcaaca caactgtaga tagctataga gtcaatgcca atggtgaatg ggtaaactaa
     3361 acttaatata actagttaat actgacttcc tgtaagaact ctttaaagta ttccctacaa
     3421 ataccatatc ctttcagtag ataatatacc cttgtaggaa gtttagatta aaaaataact
     3481 ctgtaatctc tagccggatt tatagcgcta gagactacgg agtttttttg atgaggaaag
     3541 aatggcggca ttcaagagac tctttaagag agttacgggt tttaaactat taagccttct
     3601 ccaattgcaa gagggcttca atctctgcta gggtgctagc ttgcgaaatg gctccacgga
     3661 gtttggcagc gccagatgtt ccacggaggt agtgaggagc gaggccgcgg aattcacgaa
     3721 ctgcgacgtt ttctcctttg aggttaatca atcgtttcaa gtgttcgtag gcgatcttca
     3781 tcttgtcttc aaaggtcaaa tcaggtagga tttctcctgt ttcaaagtaa tggttgattt
     3841 ggttgaagag gtaaggattt cccatggcag ctcggccaat catgactgcg tcagcaccaa
     3901 cttcttcgat gcgttgcttg gcttcttgga cagtacggat atcaccgttg gcgatgaatg
     3961 gaatcttggt tagagcttgg gcaaccttgt aaagggtctc aaggtctgcg tggccagtat
     4021 acatttgttc acgggtacgg ccatgcatgg cgagggcaga aacacctgca gcttcagcag
     4081 cgagggcatt ttctactgcc agagatgggt ccgcccagcc ggtacgcatt ttgacagtaa
     4141 gtgggatatc aaggacagac tggaccttgt tgatgataga gtagatcttg tcaggatcct
     4201 tgagccacat agcaccagct tcgttcttca cgattttgtt gacagggcag cccatgttga
     4261 tatcgacgat atcggtcttg gtgttttctt ggatgaattc tgctgcgcgt gctaggctgt
     4321 cttcatcgct accaaaaagt tggatagaga cagggttttc gccctcatcg atatgaagca
     4381 tgtgcagggt tttttcgttg ttgtattgga ttcccttgtc agagaccatt tccattacaa
     4441 cgagtccagc tccgagctct tttgcgatgg tacgaaaggc tgagttggtc acgccagcca
     4501 taggcgctaa aacggtacga ttgggaatct caacattgcc aatcataaaa ggtgtattaa
     4561 gatttgtcac gaatgagttc ctccaggtcc ttttcatcaa agttgtaagt agtttggcag
     4621 aattgacaag tgatttctgc cccgtggtct tcctctttca tttcctgtaa gtctgagctt
     4681 ggaaggctgg caagagcgtt cataaagcgt tcatggctac agtcacattg gaaacggatt
     4741 tcttcttcag aaagacgctt gtaggcttcg tccccgtaga tagccttgag gagggcttcg
     4801 atatggtcgt cgctttcgag aagagtagag atagctggca tttcttggat gcgtttttca
     4861 aagcgagcaa tctcttcttt cttggctcct ggcaagactt gaactaggaa accacctgca
     4921 accttgacct tgtcttcctc gtccaaaagg acattgaggc cgaccgctga aggcgtttgt
     4981 tggctttcag taaggtaaaa ggcaaggtct tcaccgattt ctccagagat gaggggagtt
     5041 atagagttgt aaggatttcc agtaccgtag tctgtgataa cgaggaattg accatttcca
     5101 acaaaaggtc cgactaggac ttcaccagtc gcagtctttt tgatgtcaac accaggattt
     5161 tgaacatagc ctttgacgtt ccccttggta tcagcgacgg tgataatagc acctagagag
     5221 ctagagccca acaccttaac tgtaagtttg gtatttcctt tttcattggc tgcgagaatc
     5281 tggctagcga taagagttcg accaagcgct acagttgagc tagcttgggt ttgatgtttt
     5341 tcttgagcag tgcggacggt ttcagtgcta tcaaggacaa aagcacgaaa ggctccgctt
     5401 tctgatatag ttttaataat tttatccata gctactattt tagcataaaa atgcccaaag
     5461 ggggagccgt gtgtttactg attttcagga taatggacca ggaaatcagc atgaaaataa
     5521 aaagagaaac agattatttt agcatttgta agatttatgc tatgcttaag gtagaaaatg
     5581 aaaggggtaa caaatgtatt taggagattt gatggagaaa gccgagtgtg gtcaattttc
     5641 aatcctttcc tttctattac aagagtctca gacgaccgtc aaggctgtaa tggaagaaac
     5701 aggattttca aaagcaaccc taaccaaata tgtcaccctg ctcaatgaca aggctttgga
     5761 tagtggctta gaactgacta ttcactcaga agatgaaaat ctgcgtctgt ctatcggtgc
     5821 agctaccaag gggagagata ttcggagctt gtttttgtat agtgctgtta aataccagat
     5881 tttggtttat cttctctacc accaacagtt tttagcccat cagctggctc aagaattggt
     5941 gattagcgag gctacgcttg gtcgtcactt ggctggttta aatcagattt tgtcagaatt
     6001 tgatttatcc atccaaaatg gacgctggag aggtccagag catcagattc gctatttcta
     6061 tttctgtctt ttccgaaagg tctggtcgag tcaggaatgg gaaggtcaca tgcagaaacc
     6121 agagagaaaa caggagattg ccaatttaga ggaaatctgc ggtgcaagtt tgtctgcggg
     6181 gcagaaattg gacttggttc tctgggctca catcagtcaa caacgtcttc gggtcaatgc
     6241 ttgtcagttt caagtcatag aagagaaaat gcgagggtat tttgacaata tcttttatct
     6301 tcgtttgctg agaaaggttc cgtccttttt tgctgggcaa catattccac taggagttga
     6361 ggatggtgag atgatgatat tcttctcttt tctcctatct catcgcattc ttcctcttca
     6421 tactatggag tatattcttg gttttggagg gcagttggca gatttactga cgcaattgat
     6481 tcaagaaatg aagaaggagg aactattggg ggattataca gaggaccatg tcacctatga
     6541 actcagtcag ctttgtgctc aagtctatct ctataagggc tatattttac aggatcgcta
     6601 caagtaccag ttagagaatc gtcatccata tttactgatg gaacatgatt ttaaagagac
     6661 agcagaggag atttttcatg ctctacctac ttttcaacag gggacagatt tagataagaa
     6721 gattctctgg gaatggctcc agttaatcga atatatggct gaaaacggtg gccagcatat
     6781 gcggattggt ctggatttga catctggttt tcttgtcttt tcaaggatgg cagccatttt
     6841 gaaacggtat ttggaataca atcgttttat taccattgaa gcctatgacc ctagtaggca
     6901 ttatgatttg ctggttacca ataaccctat tcataagaag gaacagacac cagtctatta
     6961 tttaaaaaat gacttggata tggaagattt ggcagggatt cgtcagttat tattcactta
     7021 aaaggcttgg ttaatccagg tcttttttgt gaaattcaca caatctcctc acattttttt
     7081 aaaaattaaa aaaagttgat aaacaagaaa gcgctttatt ttgtatacta gtaagtgtaa
     7141 agaggaaaca cctcaagatc tttatcagga ggacagtaca tgtcacaaga aaaatacatc
     7201 atggctattg accagggaac tacaagttct cgtgccatca ttttcaacaa aaaaggggaa
     7261 aaggttagct cgagtcaaaa agagtttacc cagattttcc ctcaggcagg ttgggttgag
     7321 cacaatgcca atgaaatttg gaactctgtt cagtcagtta ttgcgggtgc tttcattgaa
     7381 agtggtgtca agccaaatca aatcgaggca atcgggatta ccaaccaacg tgaaacaacg
     7441 gttgtctggg ataagaaaac aggacttcct atctacaatg ctatcgtttg gcagtcacgc
     7501 cagacagcac ctttggctga gcaactaaaa agccaaggtt atgtggaaaa attccatgaa
     7561 aagactggtt tgattattga tgcttacttc tctgctacca aggttcgttg gattttggat
     7621 catgtagaag gtgctcaaga gcgagcagaa aaaggggaat tgctctttgg tactatcgat
     7681 acttggttgg tttggaaatt gactgacggt gcggctcacg tgactgacta ctcaaatgca
     7741 gctcgtacca tgctttataa cattaaagaa ctcaaatggg atgatgagat tttggaaatc
     7801 cttaacattc cgaaggctat acttccagaa gttcgttcta actccgaaat ctacggcaag
     7861 acagctccat tccatttcta cggtggagag gtgccaatct caggtatggc tggggaccaa
     7921 caagcagccc tctttggaca gttggctttt gagccaggta tggttaagaa tacttatgga
     7981 acaggctctt tcatcatcat gaatactggg gaagagatgc agttgtctga aaacaacctc
     8041 ttgacaacca ttggttacgg aatcaacggt aaggtttatt atgccttgga aggttctatc
     8101 ttcatcgcag gaagtgctat tcagtggctt cgtgacggtc ttcgcatggt tgaaaattca
     8161 ccagaatctg aaaaatacgc tcgtgattct cacaacaacg atgaagttta tgtcgttcca
     8221 gcctttacag gtctaggcgc tccatactgg aaccaaaatg ctcgtggttc cgtctttggt
     8281 ttgactcgtg gaacaagcaa agaagacttt atcaaggcga ctttgcaatc tattgcttat
     8341 caagtgcgtg acatcatcga cactatgcaa gtggatactc agactgccat tcaagtactg
     8401 aaggtggatg gtggtgcagc catgaacaac ttcctcatgc agttccaggc ggatatttta
     8461 ggcattgaca ttgcacgtgc taaaaacttg gaaacaacag ctctaggagc ggccttccta
     8521 gcaggtttat cagtaggata ctggaaagac ttggacgagt tgaaactctt gaacgagaca
     8581 ggagaactct ttgagccatc tatgaacgaa tctcgcaagg aacaactcta caagggctgg
     8641 aagaaggctg tgaaagcaac tcaagtcttt gcggaagtag acgactaata ctggcagaat
     8701 aaagcgattt atttagaaag tgtgtaaata tggaattttc aaaaaaaaca cgtgaattgt
     8761 caattaaaaa aatgcaggaa cgcaccctgg acctcttgat tatcggtgga ggaatcacag
     8821 gagctggtgt agccttgcag gcggcagcta gcggtcttga gactggtttg attgaaatgc
     8881 aagactttgc agaaggaaca tctagtcgtt caacaaaatt ggttcacgga ggacttcgtt
     8941 acctcaaaca atttgacgta gaggtggtct cagatacggt ttctgaacgt gcagtggttc
     9001 aacaaatcgc tccacacatt ccaaaaccag atccaatgct cttaccagtt tacgatgaag
     9061 atggagcaac ctttagcctc ttccgtctta aagtagccat ggacttgtac gaccttttag
     9121 caggtgtgag caacacacca actgcgaaca aggttttgag caaggatcaa gtcttggaac
     9181 gccagccaaa cttgaagaag gaaggcttgg taggaggtgg agtgtatctt gacttccgta
     9241 acaacgatgc gcgtctcgtg attgaaaaca tcaaacgtgc caaccaagac ggtgccctca
     9301 ttgccaacca cgtgaaggca gaaggcttcc tctttgacga aagtggcaag attacaggtg
     9361 ttgtagctcg tgatctcttg acagaccaag tatttgaaat caaggcccgt ctggttatta
     9421 atacaacagg tccttggagt gataaagtac gtaatttgtc taataagaga acgcaattct
     9481 cacaaatgcg cccaactaag ggagttcact tggtagtgga ttcaagcaaa atcaaggttt
     9541 cacagccagt ttactttgac acaggtttgg gtgacggtcg tatggtcttt gttctcccac
     9601 gtgaaaacaa gacttacttt ggtacaactg atacagacta cacaggtgat ttggagcatc
     9661 caaaagtaac tcaagaagat gtagattatc tacttggcat tgtcaacaac cgcttcccag
     9721 aatccaacat caccattgat gatatcgaaa gcagctgggc aggtcttcgt ccattgattg
     9781 cagggaacag tgcctctgac tataatggtg gaaataacgg taccatcagt gatgaaagct
     9841 ttgacaactt gattgcgact gttgaatctt atctctccaa agaaaaaaca cgtgaagatg
     9901 ttgagtctgc tgtcagcaag cttgaaagta gcacatctga gaaatatttg gatccatctg
     9961 cagtttctcg tggatctagc ttggaccgtg atgacaatgg tcttttgact cttgctggtg
    10021 gtaaaatcac agactaccgt aagatggctg aaggagctat ggagcgcgtg gttgacatcc
    10081 tcaaagcaga atttgaccgt agctttaaat tgatcaattc taaaacttac cctgtttcag
    10141 gtggagaatt gaacccagca aatgtggatt cagaaatcga agcctttgcg caacttggag
    10201 tttcacgtgg attggatagc aaggaagctc attacctagc aaatctttat ggttcaaatg
    10261 caccgaaggt ctttgcactt gctcacagct tggaacaagc gccaggtctt agcttagcag
    10321 acaccttgtc tcttcactat gcaatgcgca atgagttgac tcttagccca gttgacttcc
    10381 ttcttcgtcg taccaatcac atgctcttta tgcgtgatag cttggatagt atcgttgagc
    10441 cagttttgga tgaaatggga cgattctatg actggacaga agaagaaaaa gcaacttacc
    10501 gtgctgatgt aaaagcagct cttgctcaaa acgacttagc agaattaaaa aattaagaaa
    10561 aaataaaaga ggtggagggt agcaatcctt gtcgcccgcc ccttttttaa tggagacaga
    10621 aaaatgatga gtgaattatt tggagaattt ttagggactt taatcctgat tcttctagga
    10681 aatggtgttg ttgcaggtgt ggttcttcca aaaaccaaga gcaacagtgc aggttggatt
    10741 gtgattacta tgggttgggg gattgcagtt gcggttgcag tatttgtatc tggcaagctc
    10801 agtccagctc atttaaaccc agctgtgacc atcggtgtgg ccttaaaagg tggtttgcct
    10861 tgggcttccg ttttgcctta tatcttagcc cagttcgcag gggccatgct gggtcagatt
    10921 ttggtttggt tgcaattcaa accgcattat gaggtagaag aaaatgcagg caatatcctg
    10981 gcaaccttca gtactggacc agccatcaag gatactgtat caaacttgat tagcgaaatc
    11041 cttggaacct ttgttttggt gttgacaatc tttgctttgg gtctttacga ttttcaggca
    11101 ggtatcggaa cctttgcagt gggaactttg attgtcggta tcggtctatc actaggtggg
    11161 acaacaggtt atgccttgaa cccagctcgt gaccttggac ctcgtatcat gcacagcatc
    11221 ttgccaattc caaacaaggg agacggagac tggtcttacg cttggattcc tgttgtaggc
    11281 cctgttatcg gagcagcctt ggcagtgctt gtattctcac ttttctaatc tagaaaacaa
    11341 ttatgttgat agagcttggg caagagccca atttcagcaa aaaatgaagt aaatcttctc
    11401 ataataaaac gcatcatatc aagcacgaaa attccacgag gtcaactaca gtcagaaagc
    11461 tgaacaacaa gccaaaacgc ccaaaaaagg cggcaaaaag caagcacctg caagcaacgt
    11521 gccgaaatag tcaaatcctg attatgtcaa cgaattagac ccaaaaatcg ttgatatgct
    11581 agtagaattt cacaagtcac aaggcacttt ggaaactccc gaggtgcaag cagaaatcgc
    11641 ccaaaaacgt gaagaaatcg agcaaaggag agctgagctt gagggtaaaa aacaagagct
    11701 tttgaaccgc ttgaacaaat agagtttcgc aagtattatg cttacaaatt acttgagcaa
    11761 ttaactaaaa tataaaccat acctttatat ctaggcaggg tttatatttt agaaattcac
    11821 gtaggttgtt acggttttta catacccagt atagtttgag tttctatagt attcagtgat
    11881 aaacttccat tttctttgag caacatggat ataagtactt gttatgtagt atggatatgg
    11941 gctttgtgaa tccaagtaag actgataagc ttgtatacca aaatatgctc caccaatcat
    12001 tgcaccccat ggacccccca ataaagcacc tatcctacca atcatataac tgattccagc
    12061 accagtcatg aagttagcga atgtgttagc ttgtttattc ccatgtattg tgttgacgta
    12121 attccaaaca ttaggatcgt atgatctaaa agatatattt aggtcgattt cattcttttg
    12181 ataagccata taaaatgccc cattgatata gacgccgtca gcacgtcgtt caatagtgtc
    12241 tacacttcca tctggattga caacctcaag aacttcatcg cttaaaatat ttacttgcgt
    12301 atctccgaac cgcactgatg agccattctc aaactgagcc tcaccagata caactttaga
    12361 gtttgccgat aagctatcat cagcaaaaac aacaagcgac ggggaaatgc tagacataca
    12421 gaaaacagac ataactagca aacacatgca tttaaacatc ttagacataa cggaaactct
    12481 tttgtatttt tgattttttt caacttttat tatacaataa aaccaaataa aaagaaagcg
    12541 gtaacaatat gcttaatgcg aaaatttttt atatattttt atgtttgatc gttatcgaaa
    12601 ctacaggctt gttgttgttg aaaagaggtc tcgaaatggg ttatttagac acagaagcta
    12661 ttatcctcgc agttttttca tttgcttttt acaacctatg ttcattcgct tgggtctgct
    12721 ctacaataaa aaacaataaa aaataaatag acgtattttc aaaaaaaaaa aaatgcatat
    12781 ttatattagc aaaacgacga tttaaatcgt cgtttttttg tagtacgacg ggcatgtcgt
    12841 atatctgagg tgtaagtcct cggtgggcac ctgctaccgg tgaacccaat agcgattccc
    12901 aagcctgact atcgtgaggt agcagggaga ggaaggatag cgaaatcgtg gctctacgaa
    12961 caggaacgtg atagtaaggc gtatatagcg gataaggagg cttcaaactc taaagtccaa
    13021 aaaggtagtc gtaacctata tgtgtaaatc acgagagtaa ttgaattcgg actaaggttt
    13081 gtgtgaaaaa gataaatctt tctagagtct aaagactctg cgtcagattt cctattttca
    13141 ctgtaacctt ttaacgtcct catatcttgt ataaacgagg aaagatgtac gacttatccc
    13201 gtgaggtttc atgagcgctg aaagcgtagt aacaacgaat catgagaagt cagccgagcc
    13261 catagtagtg aggaaacttc tgtagtggaa gtggagcgaa ggggtgaata ctcaaacagt
    13321 ctggggagag actgtttgag gtctgtcgct agaaagagaa aacgacagat cgaagtaatc
    13381 ctacttcact tgtgtctgta aaatgagtgg tctgatagaa ctggactttg acacgtaatg
    13441 actagatgaa ggttcaccaa tataagatgt ccctcaggca ccaaaacaag aaaggaatac
    13501 gcacatgtca aaactgctag ataagatatt atcacgcgaa aatatgctgg aagcctacaa
    13561 tcaagtaaaa tccaataaag gctcagctgg gattgatgga atgactatcg aagagatgga
    13621 taattatttc agacaaaact ggcgcttgac taaggaactg ataaaacaga gaaaatataa
    13681 gcctcaacca gttcttaaag ttgagatacc taaaccagac ggaggcatcc gtcaactagg
    13741 aattccaaca gttatggata gaatgattca acaggccatt gtctaagtca tgagccccat
    13801 ttgtgaaccc catttctcag atacgagtta tggtttcaga ccaaataggt catgtgaaaa
    13861 agccatcatg aagctcttag aatacttaaa tgacggctat gagtggatag tggatataga
    13921 cctagagaaa tttttcgata cagttcctca agatagattg atgtccttag tacataacat
    13981 tatcgaagac ggagatacgg aatccttgat tcgtaagtat cttcattcag gtgttatcat
    14041 taatggtcaa cgttataaaa cactagttgg tacaccacag ggaggaaatt tatctcctct
    14101 cttatccaat atcatgctta atgaattgga caaggaatta gaaaagaggg gacttcgatt
    14161 tgtgcgctac gcagatgatt gtgtgattac ggtcggaagc gaggagcctc taagcgtgtg
    14221 atgtattcag tcagtcgttt tattgagaaa cggctaggtt tgaaagtaaa catgaccaag
    14281 actaagatta ccagaccaag agagttgaaa tatctaggtt ttgggttctg gaaatcatca
    14341 gatggttgga aaagccgtcc acatcaagat agtgttcgga gatttaagtt taaattgaag
    14401 aaactaacac agaggaaatg gagtatagac ctaacaagac gtattgagca actgaatttg
    14461 tctattcgag gatggataaa ctatttctca ttgggaaata tgaaaagtat agtcgccagc
    14521 atagatgagc gcttgc
//
LOCUS       DAVMEI010000037        14415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVMEI010000037 DAVMEI010000000
VERSION     DAVMEI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.037"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(189..1286)
                     /locus_tag="VYQ24_000873"
     CDS             complement(189..1286)
                     /locus_tag="VYQ24_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1968034.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAMDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIAEQNFGRVPIRIETN"
     gene            complement(1296..2042)
                     /locus_tag="VYQ24_000874"
     CDS             complement(1296..2042)
                     /locus_tag="VYQ24_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1968035.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYEILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(2145..2498)
                     /gene="rsfS"
                     /locus_tag="VYQ24_000875"
     CDS             complement(2145..2498)
                     /gene="rsfS"
                     /locus_tag="VYQ24_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1968036.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(2511..3011)
                     /locus_tag="VYQ24_000876"
     CDS             complement(2511..3011)
                     /locus_tag="VYQ24_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1968037.1"
                     /translation="MKMAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(3012..3605)
                     /gene="yqeK"
                     /locus_tag="VYQ24_000877"
     CDS             complement(3012..3605)
                     /gene="yqeK"
                     /locus_tag="VYQ24_000877"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1968038.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(3605..4234)
                     /locus_tag="VYQ24_000878"
     CDS             complement(3605..4234)
                     /locus_tag="VYQ24_000878"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1968039.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(4286..4597)
                     /gene="yhbY"
                     /locus_tag="VYQ24_000879"
     CDS             complement(4286..4597)
                     /gene="yhbY"
                     /locus_tag="VYQ24_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1968040.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(4812..5918)
                     /gene="yqeH"
                     /locus_tag="VYQ24_000880"
     CDS             complement(4812..5918)
                     /gene="yqeH"
                     /locus_tag="VYQ24_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1968041.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(5921..6511)
                     /locus_tag="VYQ24_000881"
     CDS             complement(5921..6511)
                     /locus_tag="VYQ24_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1968042.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(6463..7371)
                     /locus_tag="VYQ24_000882"
     CDS             complement(6463..7371)
                     /locus_tag="VYQ24_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1968043.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(7428..7988)
                     /locus_tag="VYQ24_000883"
     CDS             complement(7428..7988)
                     /locus_tag="VYQ24_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1968044.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            8127..9335
                     /gene="sstT"
                     /locus_tag="VYQ24_000884"
                     /pseudo
     CDS             8127..9335
                     /gene="sstT"
                     /locus_tag="VYQ24_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            9403..10356
                     /locus_tag="VYQ24_000885"
     CDS             9403..10356
                     /locus_tag="VYQ24_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1968045.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            10500..10880
                     /locus_tag="VYQ24_000886"
     CDS             10500..10880
                     /locus_tag="VYQ24_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1968046.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            10979..11200
                     /locus_tag="VYQ24_000887"
     CDS             10979..11200
                     /locus_tag="VYQ24_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1968047.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            11217..11531
                     /gene="trxA"
                     /locus_tag="VYQ24_000888"
     CDS             11217..11531
                     /gene="trxA"
                     /locus_tag="VYQ24_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1968048.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            11836..12369
                     /gene="queF"
                     /locus_tag="VYQ24_000889"
     CDS             11836..12369
                     /gene="queF"
                     /locus_tag="VYQ24_000889"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1968049.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQINREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(12623..13291)
                     /locus_tag="VYQ24_000890"
     CDS             complement(12623..13291)
                     /locus_tag="VYQ24_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1968050.1"
                     /translation="MKKFVAELIGTFMLVFIGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAFSIGTVSGAHLNPAVSIAMFVNKRLSSKDLVNYILGQVVGAFIASGAVFLLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLIIGLS
                     LMAMILVGLNITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(13397..14187)
                     /locus_tag="VYQ24_000891"
                     /pseudo
     CDS             complement(13397..14187)
                     /locus_tag="VYQ24_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
BASE COUNT         4128 a         3251 c         2778 g         4258 t
ORIGIN      
        1 gttctcttca ttataccaca aaataacaag cataaagcgg attcaaagat aaagattaag
       61 gttcatatac taattttctt tattgacttt ttgtcttctg tattagggag tttatcattt
      121 tccaaaaact ttatcttcaa tttgttatct aaaatttcta caataaaaaa gcctaccttt
      181 cagtagactt agtttgtttc tattctaatc ggcactcttc caaaattttg ctctgctata
      241 cttggatttc ctagttggta aatctggtct gctttttggg tcatagcatc ccaaggttct
      301 ttgccaattc ggctgactag actgacctga cctttcaaag acttgagatg ttgcctgcct
      361 ttttcagtaa atccaaggac atgaatggct tctggcaaga cattttctct agcctgcacc
      421 aagatatagg acaagaggcg tctaacacgc gccttggtgt aacgtttggt agtcactaac
      481 tcgaccaatt cttccacaga ctgggctgtt ttaatagctt ctttaatgcg cacagccatt
      541 tcttgattga cctgatagat ggtggttagg tcgggatttg acaagatttg atagcggagc
      601 aagggaaaat agtcttccca gatcacctta ctggcctgct caaatagggc aacagaaggc
      661 ataaagcgtt ctaagaaatc ttggtccttc tgatgttgac ggagggctgt cgccgaggca
      721 aagtccacat ctttattcac agaatggtaa cctgccccct gacgctgaat cggatgcagc
      781 ttgatatttc gtcctgcaac cgccttggca taggccagag caagaacatg attgggggta
      841 ttacctgaaa aatcaagacc agcaaattcc ttccacatag cttgggtttt ctggggatag
      901 gaaagggaat caggcagatt ttccacaaat ttctccatct cagcaccttt ctctgtgtat
      961 aagtcagcaa ttttctgata atctcgaact tcctctgtcc caaaaactag actatcaatg
     1021 cccaagcgat ccaagatatc catagctcct tgaccaaaga aatcagctgc ctgaacactg
     1081 actaaaaagg gcaattccac taccaagtct gcgccatttt ccagcgccat ctgggtcctt
     1141 gtccacttat ccacaatagc aggctctcca cgctgcatga aattccctga catggcaaca
     1201 attttcagtc cctctgcctg atccagcagg tatttatggc cattatgaaa aggattgaac
     1261 tccgcgataa tacctgttat ggtcatgcta atctcctact tctgcgccac aaaaaaccaa
     1321 cgggtgctag tttctgttgg ctccttatcc tcaaagtctg catagagttt gaaggacttg
     1381 aatccagcct gttccagcaa gatctcatag gtcaagatct cataggtcct ctcctcatgt
     1441 acttcatcgt ggcgactaaa ggaaccatca gcctccttga taaagaaagt cagctcatgg
     1501 acgatggagt gaggaacttc gtcctcatat gtatcccaaa gcatggcaaa atcttccgca
     1561 ttttcatggt aggaatagcc agggaatact tcatctgtct ggtaggtcga gtgcacgtca
     1621 aagatgaaaa ctccctcttc attcagcgca ttgtacacgt ccttaaagac gtcccctact
     1681 tccacctcat cctgcatata gcagatagag tccgaataac acgtgacaaa atcgtatttc
     1741 cctgccttgg acaaattcag catattacct tctataaaag caatcttttg cttggctgaa
     1801 gttgctctct tctccgcaat cttcaacata tccgcactca agtcaagtcc agtcacatca
     1861 aaaccagcct gagagaagcg cactgattga attcctgtcc cacaagccaa ttccaagagt
     1921 ttctttctct ccttggtctt aggcaaatga cgcagagaaa aattcgtcca tttgtcgtat
     1981 aaactatcgt ccatgaccgc atcatagacc gccgcaaagg tttcataagt cgccataatc
     2041 atgatactaa gagctccatg gtaaacacca ctagcccttt acctttctat caatttttct
     2101 aaaataagca aagaaaccca gttgactaca actgagttca tcttctaagc aagagcttct
     2161 gaaatatcta ctgaattcgc ctcatgccat agcttctcta gattataatg ggcacgcatt
     2221 tcttctgaaa agatatgcac gacaacagca ccgaggtcca gtaagaccca gcctccagct
     2281 gcatcgcctt cgacatggct acctttaaag cctgcttgag ctactttttc acggatatta
     2341 gctgcgatag cgtccaactg acggctattc attgagctag tgatgacaaa gtagtccgtc
     2401 acactagtca aatcttgtac atcaagtgcg aggatatcct ccgcacgttt ctcatcagcc
     2461 gctttcacga ctagttctag taattctttt tcgttcattt aatcctcttt ttagaaaata
     2521 tgtttgtaat ctagaacatc tgcaaagcgc tcctcccaaa tgtcctcata aaattcatta
     2581 atcgtttctg ctggaatgtc atccccatca aaggttgtga cagcaccttc tggaacaata
     2641 agctgatagc catattcaaa ggcaaccttg acagaggtat ccacacaata ttctgtctgc
     2701 ataccacata aaactaattt ttcaatcccc tgcttatcca agtattcttt taagccagtt
     2761 tctttgaaaa tactgttata tttcttctga aagacctttt cagcaggttt tcgatttaaa
     2821 agcgcagata actgccaatc ttctgatgtt tgagcttcag agttctcaat atgttgaaca
     2881 tagataattt caatattctt gcttctagcc tggttttgta aataagaaat tttttccaat
     2941 agactttttg tctggaaccc agtttctact aaaacattct gaacatcaat gattataaaa
     3001 gccatcttca tttagttctc tttcaaatag tgcacaaagg cgttataggt ttcaagggtt
     3061 tggggataga tggggaatcc ctgatgagct agatactcca cggtacgagc tgtttcgtag
     3121 gccaccgcct tattgagcga tagacttgca atctcacgcg ccacatctac tccaggaaag
     3181 gctcgattgt gctcgatata gtctgctaca tagacaacct tatctaggtc tgtcatctga
     3241 ccagcaccga ctgtatggac ttcaatggct cgcaggattt ctgaatcatg caaatccaaa
     3301 tcttcctgaa ttttgtaaat tccgaccata ccatgccaga cattattacc ccagtttttg
     3361 aggtcagggt ctagctggta acggtcaatc aaagctagaa attcctgatc tgacaacttt
     3421 ttagcatagt catgaagaag acctgctaga cttgctttct caacatctac accaaagcgc
     3481 tgagctaatt ccatagcggc acgctccaca cctagacaat gggttagacg tttctcaggt
     3541 agaagctctg ccattttttc caacaaggcc tcacgggagc agttgatata gtcttgatag
     3601 gacatcagta gagcccctcc ttctcgatgt agtctagcac tggctgaggt aggagaaagt
     3661 tgggtttccg accttgggca atgaagtccc gcaccatgct ggacgagata tccatgagcg
     3721 gtacgtccac ccagataact ggataggaag tccctgcctt gtagcgtgga cgctgaaccc
     3781 ccacaaactg aaccatgtca accagttcat caattcggta ccacttaggc agatagtcaa
     3841 ccatgtcggc accgatgata aagtaataat ccgtatctgg attcttctct gtcaaaatct
     3901 tcatggtgtc gtaggtgtag gaaataccct tgcgctccaa ctcaatggtt tcaatgacta
     3961 ggccgtcaat tccctcaatt gccaactcaa gcatcttgag acgatggtgt tcagggatgg
     4021 tttccttttt atcaacgtga ggaggttggt attcaggcat gagaagaact tgatccagtc
     4081 ccaactgttg ccgtacttga tccgcaacaa tgagatgggc attgtgaaca gggttaaaat
     4141 tcccccctaa aatcccaact tgtttgcgtt ttttctcctt gatttctggc tccaactcta
     4201 ccttggtaaa gggagtcaat agttcgattg tcataggcta gtccccttta tttctctata
     4261 aaaacagtta tttggagttt cggtcttaga tttctttgac tttcttagaa atcttgcgat
     4321 tttctttctt gctagattgt ttaaacaaaa tcaagatgcg tcctattttt tggactgtat
     4381 ccacaccgat ttcttcttcc aaaatttcag ctacttcgtg gatgttttca tctgtgtttt
     4441 gtaagagagt aaccttgatt aattcacgcg catcaagagc ttgacggacg ctggttttga
     4501 tttggtcgtt gagtccattt ttcccgattt ggatgatagg tttgagggtg tgtgcctggc
     4561 tgttgaggaa ggcacgttgt tttgatgtta atgacataat ttctttaaaa gagtttcttt
     4621 ttatactttt ctgaagtggt gacggacgtc agcaaagtcc ttcggacttt catgactaaa
     4681 atttgagcct aaggtctcaa attttccgca gttggtacaa tcacttgtac caacttacac
     4741 cacagcgaaa agtattctct atggggctcg ccttgctcgc ccaaattcag acaacccttt
     4801 cctttctgtg cttaaataat tgcttttcgt gtgacgacgg cgacgccttc tggtgcccag
     4861 acggcgactt ttgctatgcc tgttacacga atccaaccta ggcctgagat gactaggtct
     4921 gtcttatctt taatggtaaa gacatgctgg actagccttg ggaaatcttc tttttccttg
     4981 ctatttggtg gtgtcagaag agttcccagg tgcttatcgt agaaagcact agctccttca
     5041 agcttgctac gatggagttt gagttcatta tcaaagaaag cagtaaatcc ttgcttttct
     5101 cctgctataa agtcaaagcg tcccaaacca cctaaaaata gggtttgctc aggattaagc
     5161 tgataggtct taggcttgat ttccttttta ggactgacat acttgaggtt tttggccgtc
     5221 aagtagtgag ccatctggtg gcggtggata attcccggcg tatcgtaaat ataagatccg
     5281 tcgtcaagcg gaatctctat tttgtccaag gttgtccctg ggaagcgtga agtagtgatg
     5341 acattctgat cacccgtgat ttcttggata atagcattga ttagagttga ttttccaacg
     5401 ttggtcacac cgaccacata gacatcgcgg cccttacggt agtgttcaat cttgtcaatg
     5461 acttccttaa tggcatgttt attttgtgct gaagttagga ccacatcgac tggacgaaga
     5521 ccttcttcat gggcacgttt catgagccac tggctaatct taccagactt aactgactta
     5581 gggaggatat cttttttatt tcctaccaag aggacatcat tgcccgagac gaaacgtggt
     5641 aaacctggga tgacagagcc attaaaatca aagatatcaa tgacattgac cactaaggca
     5701 tcactgtctc ccacctcgtg caagagcttg aggaaatcat cgttcgtcaa ctggacatct
     5761 gtgatttcat tgtagtggcg gagacggaaa cagcgttggc aatagacttc gccagtctcc
     5821 aaaccttttt caagtgccga ctggggggta aaaccaagac cagctttatc tgtcgtctga
     5881 atggttgctc cacaaccaat acagagaatt tcttccatag ttaaattcct tttttatatg
     5941 taatcggtcc gtacttttca gtgatttttc tcataacacg acgctcacga gttcggttaa
     6001 tctgcgtttt gattgagtca tgttggacca agggtttgac taaaattgac cgaatccctg
     6061 cacggtgggc tgctcgtata tctgtcatga gctggtcgcc aaccatgacc acttcctttt
     6121 tgtcatagtg gaattccttc atggcacggt caataccaaa tgtgaagggc ttcaaggccc
     6181 agtaaacgta atcaatccca aatttctcaa ctgctcgttg aacgcgtttt ttggtgttat
     6241 ttgacactac gataatgcca atacccgcgt cccgaaggtc atgtagccat tgcttcatct
     6301 ctggcgttcc atcagggttg ttccaagcaa tgagggtatt atccaaatcg accaaaacag
     6361 ccttgattcc ctgcgcctgc aggcttggga ctgtcagatc atagactgct tccacagcaa
     6421 aatctggtat ataattttca atcgccattc tggctccttt tcttaacttt ttttcgcaat
     6481 tttccttagt aacaaggata aaacaatcca caaacctgca ctagccaaac tgatatagag
     6541 ccaagcatgg ggctcatctg ttaaaggtaa gggaacattc attccgaaaa agcctgtcac
     6601 gactgccaaa acagctagca agactgaaat gatagtcaag gttgtcaaat tgtcattcag
     6661 attattgttt agaatattgt tgtaagaggc tgaaagctgc tgtaaaatct gagagattag
     6721 gtctgtcatg gataccagct gatgagcctc aatcatagca tcatcaaact gttctctctc
     6781 aatctcatca aaactacgat acaaggcatg accttgaata tgctctaaca aaatccgatt
     6841 ttgtttggca gctgccgtca gataaaccat accagtctcc aaatcagaca gggcaaagag
     6901 gtttttcttg gtagttcgct ggcgcaagag gtcattgacc tcatccctac tcttgtccat
     6961 ctgctcaatg acaggatagt aggcattgct gatgatttcc agactggcaa agagaaactt
     7021 ataaatcgaa agcgtgtcat ggctctccag ataacgagtc atctgttcaa tgacataggc
     7081 gttcttggta ttactaatgg taatcaggcg acgatgctcg acgataaagg tcatgggaaa
     7141 agtctcatag taggccttgt ccttttttac gtctaggaca ttatagataa aggtaaccgt
     7201 cccactttca cggtggtagt ccatgtgggc gcgctcgttt ctatccagtg cgtattcaat
     7261 ggtttccttg tccaaaccgt aaatttcaga aaggtcttct agtttattca actttcctag
     7321 gtctaggtct atccaggtac aaccattgcc caactgcttt tctaaaacca tcttttctcc
     7381 ttattaaact ccctctattg taccacaaat tacttaaaat aacaggtcta gtctgtgcgt
     7441 gagaaattgc taacaactgt gatatttcgg ttagggacaa agtgaagggc ttgctcctcg
     7501 ctacgcagct gtgtcgtacg aattcccaca ctgacaacct tacccgatac agtaatcggt
     7561 ccatttgtca gaacgacctc atctcccaca tccagttgac gttcaaagag gatgaaaaag
     7621 ccattgatga catcagacag aaagccttgg gctcccatac caatcgctac cccagcaata
     7681 ccagctccag ccagcaaact agaaactggc aaacctaaaa tagacaaaat gcagtagagt
     7741 aaaaagaaat atagcgtata attaaacaca ttttctagta aacgtgagat ggttttttgg
     7801 cgtccgacat catgacgaga catttttaga gaaggtttga caattctctg caccatggta
     7861 tgaagcattt ttttagcaat ataaaataca atcaaaagca gtaaaagaga aatgaccttg
     7921 gttagaatat tctcgataat ggttgtcaca tctagctttt caatataagc ctgaataaat
     7981 ttttgcatat aaaactcctt tatttcatta taccatattt tcatcagtct cattctccat
     8041 aaatattcaa aaaatagaca gaaaattctt gattttgtct atcatttata ctataatcat
     8101 aatcactaca caaaaaggag attattatga aaagaatcat tcatgcttgg aataaggcaa
     8161 gccttgtcaa gcgtatcttg attggtatgc tttgtgggga atcctaggac tgacccttcc
     8221 taatctctca gggattgggc tactcgggga tttattcgtt ggaggtctaa aagctgttgc
     8281 tcctatccta gtctttgccc tcgttgccaa tgccctttcc caacatcaaa agggacaaga
     8341 tagcaatatg aaaactgttg ttttcttgta tatcctatga tagggacttt cgccgctgct
     8401 cttgtagctg tactagcaag tttcatcgtc cctattgaaa ttaccctaaa tagtgccaat
     8461 actgaaattg caccaccaga tggggttggg caggttctca gcaacctctt gctcaaactg
     8521 gttgacagcc cagtcaacgc cctgtttact gctaactata ttggaatctt atcttgggca
     8581 gtcatttttg gaatcgctat gagagaagcc agtaaaaata gtaaagaatt gctaaaaact
     8641 atcgctgacg tgacttctaa aattgtcgaa tggatcatca atctggctcc atttggaagc
     8701 cttggtcttg tttttaaaac catttctgac aagggagtcg gaaaccttgc caactacggt
     8761 attttattgg ttctattagt aacgactatg ctttttgttg cccttgtggt caccctttga
     8821 ttgccttctt ctttatgaga cgtaatcctt accctctagt ttggaactgc ctccgtgtca
     8881 gcggtgtgac agcctttttc actcgtagtt ctgcggctaa cattcctgtc aacatgaaac
     8941 tctgccatga ccttggactc aacccagata cctattctgt ttctatccca ctcggttcta
     9001 ctatcaatat ggctggagta gcgattacca ttaacgtttt gacccttgct gcagttaaca
     9061 ctcttggaat tcctgttgac tttgccacag cctttgtcct cagtgtggta gcagctatct
     9121 cagcctgtgg tgcttcaggt attgccggag gttccctcct tcttatccca gttgcttgta
     9181 gccttttcgg tatttctaac gatattgcca tacaagttgt tggggttggt tttgtgattg
     9241 gtgtcatcca agactcatgt gaaacagccc ttaactcttc tacagatgtc ctctttaccg
     9301 ccgttgccga atacgcagca acccgtaaaa aataactcat caaggcaagc ctgctcatgt
     9361 cttgtctttt acgcttttat tctaacttat taggaaattc ttatgtctat tagccaacgt
     9421 acgaccaagc tcatcttagc tacctgtctt gcctgcctgc ttgcttattt tctcaatctt
     9481 tcatcagcag tttcggctgg aattatcgct ctcttgagcc tatctgatac gcgtagaagt
     9541 actttaaaac tggctcgcaa tcgtcttttt tctatgcttc tagctctggc tatcggtgtt
     9601 ctagcttttc acttgagcgg atttcatatc tggagtctcg gcctctatct ggccttctac
     9661 gttcctttag cctacaagat gggctgggaa attggcatca caccaagcac tgttttggtt
     9721 agccatctct tggttcaaga gtcaacctct ccagaccttc tagtcaatga attccttctc
     9781 tttgctattg gtacaggatt tgccttgctt gttaatctct atatgccttc acgagaagag
     9841 gaaatccagc actaccacac gctggtggaa gaaaagttaa aagatatcct ccagcgcttc
     9901 aaatactatt tatccagagg agacggacgc aaccgagcac agctggtagc agaattagac
     9961 acgcttttga aagaagccct cagactggtc tatttggatc actctgacca cctctttcac
    10021 cagaccgact accatatcca ctactttgag atgagacaac gacaaagtcg tatcctgaga
    10081 aacatggccc aacagattaa cacttgtcac cttgccgcca gtgaaagcct gatcttagcg
    10141 caactctttt caaaaattgc aggtcaactg agccagacca atcctgcttc tgatttgcta
    10201 gatgaaattg aacgttatct ggaagtcttc cggaaccgca gtctgcccaa gacaagagaa
    10261 gagtttgaaa cccgcgccac ccttcttcaa ctcctacgtg aagccaaaac cttcatccaa
    10321 gtaaaagttg atttttacca aaaatatgga cagtaaaaaa taaaaccact aaaaaacaga
    10381 gcttgccagc attcttgaaa agtagtataa ttattgcatg cgcaatcatc ttgtgacgca
    10441 gagactgccc gttaatggac tttcttctat tttatgactc taaaaagaaa ggagacacta
    10501 tgacctattt ggaaaaatgg tttgacttca atcggcgtca gaaagaaatt gaaagtctct
    10561 tggaagagac cattgcccaa cagagcgagc aaagtctgac cttgaaagag ttctacctgc
    10621 tctactatct ggacttggct gaagaaaaat ctctacgcca gattgacctg ccagataagc
    10681 ttcatctgag cccgagcgca gtttctagga tggtggctcg cttggaagct aaaaattgcg
    10741 gtctactcag tcgcatgtgt tgccatcaag atagacgctc tagttttatc tgcctgacaa
    10801 atgatgggca aaagacactg gcctcgctac aaaagactgt cgaagaaagc ttggaagcag
    10861 gtttggattt cttgatttaa gatgatttta aaaaaagttg cgtgcgcaat catttttctt
    10921 gacattctct tttataagga gtaaaataaa gttagcatta aacaaaggag ttttaaatat
    10981 gattgaaatt acctatatag atgccagcaa gaacgaaaga actgtgactt tcgagtctta
    11041 tgaagacttt gaacgttcgc aacaagcttg ccttatcggc gtcgcagact actaccctgt
    11101 ccaaaaatta acttacaagg gccataattt ggactaccat gggacttatg gagatatctt
    11161 cttctatctc atgaaacaag atttaagcca atataactaa aaaaaggaga aatacaatgg
    11221 caaaagcaat tacagatgca acattcgaac aagaaacaaa agacggtttg gtcttagtag
    11281 acttctgggc aacttggtgt ggtccatgtc gtatgcaagg tccaatcttg gacaaattgt
    11341 ctgaagaact ttcagaagat gtcttgaaaa tcgttaaaat ggacgttgat gaaaatccaa
    11401 acacagctcg tgcttttgga atcatgtcta ttccaactct tctcttcaaa aaagacggcc
    11461 aagttgtcaa acaggttgca ggtgttcaca cagcagaaca aatcaaggcc atcattgctg
    11521 aattgagcta atcaaacgag agaccaagta ctttctttgg tctctttttt cttgcccttt
    11581 gccatttttc aaaaaatatg ctagactata gcagattgaa actagaatag tacacctcta
    11641 cttctaaaac attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttcttatt
    11701 tcattttact atataagtag aatattacga tgtttgggat atgccatcgc taaaaaaact
    11761 cgatttggta ttttttagct ccctaatggg agctttttgc atgctctgaa catttcccat
    11821 tttggaagga gtactatgaa acgtcaatca gccttggtcg tctttagtgg tggtcaagat
    11881 tcaacaacct gccttttctg ggccatgcaa cactatgaaa cagtcgaagc tgtcaccttt
    11941 gcctacggcc aacgtcatca cctcgaaatt caaattaata gagaaatcgc taaggaacag
    12001 ggcattcgtc accatatcct cgatatgtct ctgctgggac aaatcactgc tcagccagac
    12061 tttgcgacga ttcatatttc ctacattcct gacaagctct gtgtcgagtc aaaatccctc
    12121 aaactatatc tatttagcta ccgaaaccac ggagatttcc acgaaaactg tatcaacacc
    12181 atcgggaaag acttggtcaa cttgctagac cctcgctatt tagaagtctg ggggaaattc
    12241 actccgcgcg gtggcatttc aatcgacccc tactacaact acggtaagca aggaactaag
    12301 tatgagggct tggcagaaca acgcctcttc caacacgacc tttatccaga gaaaattgac
    12361 aaccgctaaa gtcatactca atgaaaatca aagagcaaac taggaagtta gccgcaggtt
    12421 actcaaagca gtgtttgagg ttgtagatag aactgacgaa gtcagcttaa aacactgttt
    12481 tgaggttgca gatagaactg acgaagtcag taaccatacc tacggcaagg tgaagctgac
    12541 gtggtttgaa gagattttcg aagagtatca tacgaaaaag ccctgttcct caagatgagg
    12601 agcaaggctt tttgagtttc aattattctt ctgttccaag gaaatttttt gcaacaaggg
    12661 ctgcaagaac tccaccagcg attggtgcaa ggatgaaaat ccaaacttgt tgaagggctg
    12721 cgccgcctac caagacagct ggtgccaagc tacgagctgg gtttactgaa agtccagtaa
    12781 tgttcaatcc gacaaggatc atcgccatca aggacaaacc gattatcaaa ccagcaatcg
    12841 cgccatttcc tttactttct gaagtcacag tcatgataac caaaacaaac aagaaagttg
    12901 cgatgacttc aaacaagaaa ccaccaaaga cagtgacacc gtttgccaaa gcattttcac
    12961 caagactagc agttgacata cctgaatttg ccaagagcaa gaagacagcg ccagaagcaa
    13021 taaaagctcc gacaacttgt ccaaggatgt agtttacaag gtcttttgat gacaaacgct
    13081 tgttaacaaa catagcaatc gaaacagctg ggttcaagtg agcacctgaa acagttccaa
    13141 ttgagaaagc tgcgactacg attgccaaac caaaggcaaa agcaattcca aggtgaccaa
    13201 ggccatcaaa accatttcca aaaacaacag ctcctgttcc gatgaacaca agcatgaacg
    13261 taccgattaa ctcagcaaca aattttttca ttttctttct ccttttttca aaaactagat
    13321 actagtctat caaaagaggg aaagggtttc aagaaaattg cttggaaagt tttttgaaaa
    13381 tctgactcgc tttttcttag tattgaaaag actggatagc ttctttcaag tcatcttgta
    13441 aactatttct ctggtcaagt tgaacataga cttccaacag acaggatctg aagttggaaa
    13501 atttataaaa atcttctctt tcttctatcg gaaagtcaac agcttttatc caagaagcta
    13561 cttgctcttg cgctaacttc ccttgtaaaa taggttcata gatcactctt gctaaacgcc
    13621 aatcctcatc atctgtaaag cgaatcgaaa ctcttttaaa tagttggcca agtatatcaa
    13681 atacttcatg aactctgttt ttaggaaagt tgggatgaca aaccacctct gtcagtaaat
    13741 cggctccatg tgcaaaagca tgaacccaac catactgact tgaaaaaccc gttgtatcct
    13801 tttcttttga aagataatac aaaccttgat ttaaaaggac attacgaatt cctgcattta
    13861 attcctgata aaaatcgatt gctggttggc atctgcagac aagagatttg cataaataag
    13921 cgccctaaaa gaacgttcaa gggttgacag gcctacctta tcaatatctt tatctaaccc
    13981 tccatcagat gaaatccctt cagcaatgaa atgaaattgt tcctgtgtaa atagctcttc
    14041 ctgaatccct ctagcaaagc ttgtaaaaac aaggtcatca cgaatttctg gagaaggatc
    14101 tcccaaatga tcaagtagcc actggatttc ttcctggtta taacttggtt tttcttctgc
    14161 tatttttcta agtaactttt gatacatggt caatacctct acatttctag caactgttca
    14221 aaaagctgtc cttaaatgac tttagcaatt tgtaaaatta agttaaagag aaaaagacat
    14281 ttatattcag agaaacagca catttctgaa aggatacata aatgatttat tccatattac
    14341 cacaaatgaa cttgtaatga tagaacttta tcacaaaaat cacatttctg tctcaaaaat
    14401 tgcgaaaaac tagtt
//
LOCUS       DAVMEI010000038        14169 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVMEI010000038 DAVMEI010000000
VERSION     DAVMEI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.038"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(272..1879)
                     /locus_tag="VYQ24_000892"
     CDS             complement(272..1879)
                     /locus_tag="VYQ24_000892"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1968051.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPVADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYANDAEVKINWINAND
                     VTAENVVELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDVEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2269..2856)
                     /locus_tag="VYQ24_000893"
     CDS             complement(2269..2856)
                     /locus_tag="VYQ24_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1968052.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2984..3433)
                     /locus_tag="VYQ24_000894"
     CDS             complement(2984..3433)
                     /locus_tag="VYQ24_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968053.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3438..3827)
                     /locus_tag="VYQ24_000895"
     CDS             complement(3438..3827)
                     /locus_tag="VYQ24_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1968054.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3855..4481)
                     /locus_tag="VYQ24_000896"
     CDS             complement(3855..4481)
                     /locus_tag="VYQ24_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1968055.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVIFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKNPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVFASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4495..5058)
                     /locus_tag="VYQ24_000897"
     CDS             complement(4495..5058)
                     /locus_tag="VYQ24_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1968056.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5223..5457)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5511..6014)
                     /locus_tag="VYQ24_000898"
     CDS             complement(5511..6014)
                     /locus_tag="VYQ24_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1968057.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFMSQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6207..7037)
                     /locus_tag="VYQ24_000899"
     CDS             complement(6207..7037)
                     /locus_tag="VYQ24_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1968058.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7022..8704)
                     /locus_tag="VYQ24_000900"
     CDS             complement(7022..8704)
                     /locus_tag="VYQ24_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968059.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8806..9063)
                     /locus_tag="VYQ24_000901"
     CDS             complement(8806..9063)
                     /locus_tag="VYQ24_000901"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968060.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9070..9618)
                     /locus_tag="VYQ24_000902"
     CDS             complement(9070..9618)
                     /locus_tag="VYQ24_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1968061.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9633..10481)
                     /locus_tag="VYQ24_000903"
     CDS             complement(9633..10481)
                     /locus_tag="VYQ24_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1968062.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10670..12019
                     /gene="trkA"
                     /locus_tag="VYQ24_000904"
     CDS             10670..12019
                     /gene="trkA"
                     /locus_tag="VYQ24_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1968063.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12023..13462
                     /locus_tag="VYQ24_000905"
     CDS             12023..13462
                     /locus_tag="VYQ24_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1968064.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13587..13844)
                     /locus_tag="VYQ24_000906"
     CDS             complement(13587..13844)
                     /locus_tag="VYQ24_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV1968065.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         4156 a         3236 c         2612 g         4165 t
ORIGIN      
        1 ctgatagaga tgttcatatc ttatttcaat tcactataca atccagttcc taaaactttg
       61 ctagaaattg atttggcttt ccgaattaat ttgttcagat tttatttcat ttcaatatac
      121 tgataaatcc attcactagc acagggcaag agggagtcgc tattgatttc aagggtagca
      181 ggcacataca aaggactcct acctcagata tactgcaata tccatcgtat gcaaaaaacc
      241 tctgagattc ttctcaaagg ttctgatttt gctaattgct gttctcaact gctgcagtga
      301 caaaggcagt gtagagctct tctgggcgat ttggacggct tgacagttca gggtgatact
      361 gacaagccac aaagaattta ttttctggaa tttccacgat ttctaccaaa cgattgtctg
      421 gagaaactcc tgagaagaca aaacctgctg cctcaaactg ctcacggaaa gcgttgttaa
      481 actcatagcg gtgacggtga cggcgttgca ccacttcttg attgtgataa gcagctgctg
      541 ccttagagcc acgttttagt ttagacggat aaagtcccaa acgaagggtt ccccccatat
      601 cctcaacatc aatctgatca cgcatgatat caatgatagg gtattttgtt tctggtgcaa
      661 gctctgcaga attggcacct tcaagaccca aaacgtgacg agcaaactcg atacaagtca
      721 actgcattcc caagcagact cccaacattg gaacatcatt ctcacgcgca tagcggatag
      781 cttggatttt cccttccgta ccacgttgac cgaaaccacc tggcacgatg attccgtccg
      841 catcagacaa aagctctacc acattctctg ctgtcacatc attggcattg atccaattga
      901 ttttaacttc tgcgtcattg gcataaccag agtgtttcaa agcttcaacc acagagatgt
      961 aggcatcttg caactcaaca tacttaccga caagggaaat tttaacttgt ttcttgagat
     1021 tcatgacctt gtccaccatt gctgaccact ctgtcatatc agctaccggt gcgtctaatt
     1081 tcaaatggtc acagacaatt tgatccatac cttgagcctg caagttcagt ggaatttggt
     1141 aaagatgttc gacatccaac gattcgataa cggcttctgg tgccacatca cagaactggg
     1201 ccagtttgtt tttaattcct tgaccagctg gctcttctgt acgaataacc aacatatttg
     1261 gttgaattcc caaaccgcgt aattccttga cagaatgctg agttggtttg gttttcattt
     1321 caccagcagc cttgaggtaa ggaagcaagg ttgtatggat gtacataaca ttatccgccc
     1381 ccacatctgc cttcatctga cgaagggcct ctaggaatgg caaggactcg atatctccta
     1441 ctgttccacc aacctctgtg ataatgacat cagagtcggt cgttagagcg gcacgcttaa
     1501 ttttttcttt caaagcatct gtgatatgag gaataacctg aacagttgca ccaaggtatt
     1561 ctccacggcg ttccttacga agcacttcac tgtaaatttt accagttgtc acgttggaat
     1621 atttgttaag attgatatcg atgaaacgtt catagtgacc caagtccaaa tctgtctcag
     1681 ctccgtcatc tgtcacaaaa acttccccgt gctggtaagg actcatagtt cccggatcaa
     1741 tattgatata agggtcaaac ttttgaatgg ttactttgag accacgattt ttcaagagac
     1801 ggcccagact cgctgccaca atccctttcc caatagacga tacgacacca ccagttacaa
     1861 aaatatattt cgtagacata gattcctctt tctaaaatgc tcaaggtctt gttaactacg
     1921 aggggatata gaaaacaaga ccctactaat aagaataatc tgggacgact gcaccagatt
     1981 cacaactaat actcttcgaa aatctcttca aaccgcgtca acgtcgcctt gccgtagata
     2041 tgtgttcctg actttgtcag tcttatctac aacctcaaaa cagtgttttg agcagcctac
     2101 ggctagtttc ctagcttgct ctttgatttt cattgagtat aaaatacaag aagataactt
     2161 cttgaaaaaa caaaaatagc tccctaagaa ctagggagcc ccgacctcta aaagaggtgc
     2221 ccaaacaata tgatacctaa aaataggata attgtcaata gctaactctt attcctcgct
     2281 ggtttcagcg tcgtcatctg aaaactcgtc gtcgtcttcg ttgagatcca catcttctcc
     2341 caagtcatct ggagcgattt cgttgatttc tgcatcataa gcttccactt catttttttc
     2401 atcatctgga ttttcatcat cgtatgaaag agctggatct gcttcgtatg catcttcgtc
     2461 ttctggatca tctgcattgt agtcaatggc atctgaatca ccatccataa aggcattgac
     2521 acgttttttc ttagcttttg gtgctacttc atcgtcgtca ttttcttcaa gagcgatgat
     2581 ttcttcgtcg atttcgtcca caccatacca tgaacgaaga ccccatttgt tgtccccaag
     2641 tgagatgaag ctaccgtcaa agttcaactc tgtgtagaac aaaggcaaag cttcgcggat
     2701 atcgctgttt gatgttccaa ggtagttttg aatttcgttt acaagatcgc taaaatgcat
     2761 ctcatgatcg cgaccacgaa gttctaagat agcacgcgct acctcaatca tagatagttc
     2821 acttttttct tgcccagcaa atacttctaa ttccaaagcg tttctcctca tttatactac
     2881 tatcgtcaga gcgaacagac tctgacctca ttttatcatt tactctttat tttacgataa
     2941 ttttgcggaa tagtcaaagg ttaaggggga gaaagtggca ggattagact aattccaata
     3001 taaaactcct tcctttttct gttgctccat tttccacaaa tccaagcgac ttgaaacacc
     3061 tcctagaagc atgattgtag gtgtagattt ccttgactct caattctttc catcctttta
     3121 ctcgagccaa ttcaatcaaa gcacttagaa tcttttttcc aagtcctcga tgttggtaag
     3181 cggaattccc aatcacaatg gggagattat cctgagatag tgtaatatcc ccaattggaa
     3241 accattctcc cttctccttg acttcaatcc aaaaaagctc accatgccga tccaaatagg
     3301 aatacatggc ttccaaggtc gcttgactgt aaggaagctt caccccatct acgaggtaaa
     3361 ccaagttcac atcctgatac caagcaaaag cttcctcaca atgattgacc ttataataag
     3421 gaacaaaaca caatgcacta ccttttcaac aaaagagttg ctgcttgatt aaaaccatca
     3481 caccagttat accattttgc ttcataccca tcttgagcta ggatacgatc ttctaaatca
     3541 aaaacagagt aaatctttct ttcctcgcaa gcttgcgcat agagatgata tagttcatca
     3601 ccaccatctc tatcccactc agcagaaatc gtatcccgac ctgccaataa agcctgataa
     3661 gccctgtgat gcccatctgt aatcagcaaa caatctccaa aggcaagaat actgattgga
     3721 tcgacttggt ttgtttctgc cgactggtaa agcatctgaa tatcttgcaa cttcttttct
     3781 gataaatata gttgagtcgg atgaagatct gctatattga ctttcatttc tttctcctca
     3841 agggaattcg atactcactt ctgtttgcct ttaaatcgcc attggaagcg gagcttgtca
     3901 tagaagggaa actcgataaa caggactccc aagcccacac agagactggc aaagacgtct
     3961 gatgggtaat gaacccccag atagactctt gataccagca cactgactag gtagaggcca
     4021 aggacgattt gtacgatttt tctccagact ggatttttaa ttcgttgact gagaatgaca
     4081 atcagagtac ctaccatcaa agttacagcc agagaatgcc cacttgggaa ggaaaatccc
     4141 ttctcttcca ccagatgtaa gatagctggt cgtgggcgct ggtagatatt tttaaagatc
     4201 acgattaaaa gacctgccaa agccagattt cccagcatga agaaactttc tatcttccat
     4261 cgcttacgat aaaagacaaa agctgtaatg acaacccaag tgataatcac tgggatatca
     4321 atcagacgtg tgagggctcg aaaaagaata gtcaaataat ctggtaagtc tcctcgaatg
     4381 gcagtctgaa tcgattggtc aaaattgacc aacatttcag ggtaaaattt gaccatatag
     4441 ccaagaataa cgaaaagtaa aagggcaaaa ctgcccttca ttaaaaatgt ttgtttatct
     4501 ttcatagtgt tttaaggttg gtttcaaaag gacgtacaac aaccagaatg aaacggaaaa
     4561 gattacaccc tcaatcaagt taaaaggtaa taccatggtc attaggtagt tggaaagtcc
     4621 caaaattttt ccaatatcaa agttagcaaa cttagcgtac aaaggaacag cataaacata
     4681 gttgagaacc aacatggcca aggttaaacc aacagttcca gctagagagc ctagtaggaa
     4741 acgaagggtt gtccgttcct ttttccaaat caaagcaaat acgatgacaa aaactcccaa
     4801 agctacgata ttcatcggca aaccaatgta agtattcact ccttggctgt taagaagcaa
     4861 tttcaatagt gagcgaagca agagcactcc tagagcagca ggcaaatcca tgaccaccag
     4921 acccacaagg actggcaaga tactaaattc gatcttgagg aaagatgccg ctggtaaaag
     4981 cggaaagtca aagtacatca gcacaaatga gatggctgat agaattgcaa tggtcgaaag
     5041 tcgacgtgtg tttgtcataa caggttcctc caattttcta taaaatcaga agaagttgga
     5101 aaggattcct ctatctattc tcacttttta tatcccaaaa gttccctctt attctattaa
     5161 agaaaaacaa agcaagtggt tacaatccgg ctataaatct atcaaaacag acaaagctat
     5221 tctttcgtct tctcccatcc agactatact gtcggttgtg gaatctcacc acatcagctt
     5281 gcgctcgcgg acttctttaa agagaaggaa atagagattt ttcttttaga ctaggcgaca
     5341 agctgtaggc ataacttctg gttaggcaaa gcttgtcaac aaagtataaa aggaaaagat
     5401 catttcctag ttaaagtcac cgccggtcgg gaatcacacc ctgccctgaa gactctttta
     5461 tcataacaaa aaacgcttgc aagcgcaagc attttgttca ttttcatttt ttatttcaat
     5521 ttatccactt cataggtatg gacgagctca agacctgcaa agttctgctg gcgaagagcc
     5581 tcatagacaa tcatgcagac ggtattagac acattgagac tgcggacatg ttcatcattc
     5641 atgggaatac ggagagcctt ctcaggatgt tctcgcataa aatcttcagg taagcccttg
     5701 tcttcacgtc caaagagaaa ataatggtcc tcgtcagtcg ataaatccac ctcagaatag
     5761 actttttccg cgaatttcga aatcagatag agttttccct tcatctgaga catgaaatct
     5821 tccaaactct cgtaaaaata aatctctagc ttatcccagt aatccaatcc agcccgcttc
     5881 atcttgcggt catcaatagg aaagcccata ggcttgatga tgtggagggg agaattggtc
     5941 gcagcgcaag tacgcgcgat attgcctgta ttttgtggaa tttgaggttc aaataataca
     6001 atgtgatttg tcatgacttg cttcctttca ccattgcaaa aaaatagcca cactgcccgg
     6061 agtcaagctc agcaaacagc gtggttaagg catcgttaac ttacctcaca acaggtttga
     6121 agtaaatcag cgaaactact ttcttagtat aacactttca gaatcattgt caatagaaat
     6181 gacttgattt tttcaatttt ttcaagctat ttccaagggt tgtaaaatcg tccctgattc
     6241 tgcaagataa gtagtaaact aactactaaa aacaaggttg ccaagagcaa ggtaatatag
     6301 tctccttttt tcaaggcctg ataactatac catgtgcgtt ttttctcttt cccaaagcgg
     6361 cgaagctcca tggcagtcgc aatagtatca atgcgttcta gcgagctaaa aatcaagggc
     6421 gtaataatga gcagattgcc tttgattcgt tgcataagag aagctttctt ggataattcc
     6481 atcccacgcg cctcctgaga catcttgata gtaaagaatt cttcctgcaa atctggaata
     6541 tagcgcaagg tcaggctgac agaataagca atcttatagg gcacaccaat ttgatttaaa
     6601 ctggaagcaa actgactagg atgggttgtc atcaaaaaga taatagccag aggaatggtg
     6661 caaagatact taatggccaa atttagcaga taaaagagct cctggctggt tagagtgtag
     6721 acaccgattc cctgccaaat cacacttctc tctccataaa gtccaacccc atactcggga
     6781 gaaaagagat agaccatcaa aacgtttaaa acggcaaata ccgtcgcaaa aacggctaca
     6841 aaggaaacat ctttaaagcg aatttctgat aaatagagga gaaagactga aaagatggca
     6901 atcagcaaga gcattctggt gtcatagcta atcatggccg ccaatgatac cagaatgaaa
     6961 aagagaagtt tcccagctcc tgacaagcga tgaatcacag tatctctatg ctggtaaccg
     7021 attaatttag cttgcatccc tctctccttt ctttgtaaaa tgccgttaaa tccagtggat
     7081 ccacatctag tttcttagcc aagttaaaga tggaggtttc ttttagattg gcttttacta
     7141 acagctcagg atcgctcaac agactggctg gaacagtatc ggcaatcaat tctccatcca
     7201 ccatgacaag gacccggtct gaataatcca gcatcaattg catatcatgg gtaatcatga
     7261 caatggtatg ccctttttga tgtaactctt cgagaaattc cataatctca gtatagttct
     7321 tctgatcttg acctgcagtc ggttcatcta ggagaataat ttcagctcct aagaccaaaa
     7381 ttgaagcaat ggtgacacgt tttttctgac caaatgacag ggcagaaata ggccaattac
     7441 ggaattcata aagtccacag attttcaagg tttcatagac tctcgtttca atttccttct
     7501 catccacacc tcgcaaacgg agccctagag ccacctcatc aaaaatcata ttggttgaaa
     7561 tcatttgatt aggattttgt agcacatagc ctactcgttc cgcccgctct gcaacagaat
     7621 cgccttttat atcctgtttt tcccaaagat agcgtccttc cgtctgaata aagctactta
     7681 tagccttggc tagagttgat ttccctgctc cattttttcc gacaatagca atcttttcac
     7741 cctttttaat atctaaatgt agggatttta aaatcggtct atcatcataa gaaaaagata
     7801 cttcctctag tctaaagagt gactgcaatg ctggggtttc ttttaccagt tccgtctgta
     7861 actggacttg cccttttgag atagacaagt tatccagatc tgctaattgt tcttccttga
     7921 ctaagtccac acctaattga cggagagtcg ttagataaag gggttctcga attccatttt
     7981 gagtcaataa atcagtcgca agcaactggt cagggctccc attaaaaagg atacgaccat
     8041 cgtttatcaa gacaatccga tccacagggc gatgcagaac gtcctccaaa cggtgctcga
     8101 taatcagagt cgtcgtcccc tcttccttat gaatctggtc aatcaattcg ataatatcct
     8161 gacctgactt gggatctaga ttggcgagtg gctcatcaaa caagagaatc ggactttcat
     8221 caatcaagac accagccaga ctgactcgct gcttttgtcc acctgacaaa tcctgaggac
     8281 gctgatccag taaaggaaga aggtccagct tttcagccca tttataaaca cgacctttca
     8341 tctcatctag ggctgtcaca tcattttcca gagcaaacgc caaatcttct gccacagaca
     8401 agccaataaa ctgcccatct gtatcctgca aaactgtgct aaccagatga gacttatcat
     8461 agatgctcat atcaaaggct gtttgaccct ttatcaaaaa ttctccatat gtctgaccct
     8521 tgtaaatatt gggaataatc ccattcaaac actgacccaa ggtagattta cctgatccag
     8581 atggtccaac aattaagact ttttctccct tgtaaatggt caagtctatc ccttgcaagg
     8641 tcggttcttg ttgtgtttca taccggaaag agaaatcctt ccactcaatt atagcttctt
     8701 tcatcttact ctcttcattc gcttcttaga cttctatttt atcataaatc aagcccttct
     8761 tgcagtctct tctcttaaaa tcttagcgcc aaaaagattc ctatcttagc ttacttgcct
     8821 aactaatcta taaacatcga aaaagactag ttgcccagcc ttccccatca ttttatactc
     8881 ttcgaaaatc tcttcaaacc acgtcagctt cgccttgccg taggtatggt tactgacttc
     8941 gtcagtttca tctacaacct caaaaccatg ttttgagctg acttcgtcag ttctatccac
     9001 aatctcaaaa cactgttttg agcaacctgc ggctaacttc ctagtttgct ctttgatttt
     9061 cattgagtat tagtcctttt tcaaacttcc tgcacgagtt tgagttcctg cataggcaag
     9121 taagagaaga gttcctgcaa tagctacaga tacaccattg gcaattcccg caacaatccc
     9181 ttgtgcaaat actttttctg ccgcttcttg ataaatcaca acatctccaa gtggtgccaa
     9241 gacaccccaa acaagggcat ttgcaagtag ttgaatgagg ttaaaaataa gaatatcttt
     9301 ccagtcaaaa acaccattga tcactcgaac gtactttcta aaaagtccca caactaaacc
     9361 aaagagtccg ctagcgataa tccaagtcca ccatagacca taaccagcaa gagagtcctt
     9421 gattgcatga ccaatcaacc cgacaagcaa accgataatc ggtccaaaaa taatagaaag
     9481 tagcgcttgt accgcatact gaagctggat gcttgtattt ggaacagggg ttggaatgtt
     9541 gatcatcccg atgacgacaa agagggcagc gccaattccg acagcaacaa cttgtttaat
     9601 tgtaaatttg atttccatac tattctccta ttttatcctt ctattttctt tatttcaatg
     9661 gtccaagatg aaccgacacc tacattatag gccttggcaa aggaaccttg gttgatagcc
     9721 aaacctaaac gatagagaga gttgatgtaa aggatgggtt gcccaattct cacatctgca
     9781 aatgatttgc catagacaac ctgattttga tagaccagca tatcagcatg atagatggtc
     9841 acttcaaaac gatcaccaaa ttctggttcc agcttgtaaa attcttcccg tgtgatagag
     9901 gtccaaagcg aaccgaaacg cacatccaga atatcaatgg ctcccttcac cagatgatct
     9961 tctatgatgg tcgctacgac tggaagctct acaatctgtt ccacactgag ctctggccct
    10021 acttcctcaa aagtaatgtg accactggcc agtttagcac cagtataggc atagacatca
    10081 cgaccgtgga aggtataaga atgctctgtg ttttgacgcc tattggccac ctcagaaatc
    10141 tcacgaatgg ctacaatgcc aacgtgtttc ttgataaagg aaagcgtccc attatctggc
    10201 gtgacaatgt attgattttt tgcagtcttg gcaactacac tcttacgttt cgaaccgaca
    10261 cctggatcga caaccgatac aaacgtcgtt ccctcaggcc agtaatccac cgtctgaaag
    10321 agacgatagc tcccctcgaa aatattataa ggcgtgatat cgtgcgtcaa gtgatggatt
    10381 tttaaggttg gagactcttc taaagccact ccaatcatag ccgataccgc accatcaacc
    10441 aaaccaaagt ctgattgtaa taccagtaaa ttattattca ttttatctcc ttgtatatcc
    10501 tttatcatac catatttcag agaaagtcgc taatagctat ttcaattggt tagggtatag
    10561 ttttacctta tagcaaattt cctactaaaa gttcttgtta ttagctagta ggtgcatttt
    10621 ttcttgaaaa agggtatgat agttacatca tgaaaaagta ggttttatta tgaaaattat
    10681 ccttgtcgga gggggaaaag ttggttttgc cctctgtcgc tccttggttg cagaaaagca
    10741 tgatgttttg ctgattgagc aagacgaagc tgttctcaat catattgtca gtcgctttga
    10801 tatcattggt atccttggta acggggccga ttttgccatt cttgagcaag ccagcgtcca
    10861 agattgtgat atctttatcg ccctgactga gcacgatgaa gtcaacatga ttgcagcagt
    10921 tctagccaag aaaatgggag ctaaagaaac tatcgttcgg gtgcggaacc ctgaatattc
    10981 taactcttat ttcaaggaaa aaaatattct cggtttttct cttatcgtta atcctgagct
    11041 cttggctgcc cgcgctatcg cgaatatcat tgacttcccc aacgccctgt ctgtcgaacg
    11101 ctttgctggt ggacgcgtta gcctcatgga atttgtcgtc aagtccacca gcggtctttg
    11161 ccaaatgccc atttctgatt ttcgtaaaaa atttggtaat gtcattgtct gtgcgataga
    11221 gagggatcat caaattatca ttccaagcgg tgacatgact gtacaggata aagatagaat
    11281 ctttgtcact ggtaaccgtg tcgatatgat actcttccat aattatttta aatcacgcgc
    11341 cgtgaagagc cttctcatcg ttggggcagg tagaattgcc tattatctac ttggtattct
    11401 caaagatagt cgtatcgata caaaagtcat tgaaatcaat cctgaaatcg ccagcttctt
    11461 tagcgagaaa ttcccaaatc tctacatcgt tcaaggagat ggaaccgcaa aagatatcct
    11521 gctggaagaa agtgctcaac actatgatgc cgttgcgact ctaacaggag tcgatgagga
    11581 aaatctgatt acttcgatgt tccttgacag ggtaggtgta cagaaaaata ttaccaaggt
    11641 caatcgtacc agtctcctcg aaattatcaa tgcgcctgat ttttcaagta tcatcacacc
    11701 taaaagtatt gccgtggaca cgattatgca ctttattcgt ggtcgtgtta atgcccagta
    11761 ttcagacctt caggccatgc accatctagc caatggtcaa atcgaaaccc tgcaattcca
    11821 tatcaaggaa gccaataaaa tgactgccaa acctctttct caactgaaat taaaaaaagg
    11881 tgttcttatt gcagccatca ttcgaaaggg caagactatt ttccctactg gggaggatat
    11941 gttggaagtc ggagacaagc tcctagtaac aaccttgttg ccaaacatca ccaagattta
    12001 tgacttgatc gcgaggtaag aaatgaataa aagtatgatt cgttacctcc tttcaaaatt
    12061 acttttgatt gaagctgttc ttcttttggt tcctgttgcg attgctgtct attaccgtga
    12121 atcgagccaa gtctttacag ccctcttttc gaccataggg attctcgtat tgttaggcgg
    12181 ttcagggagt ttacagaagc caaaaaatca acggatttat gccaaggagg gagtcttgat
    12241 cgttgccctc tgttggatcc tttggtcttt ctttggtggt ctcccctttg tcttttctgg
    12301 gcaaatccct agccttattg atgccttttt tgaaattagt tctgggttta caactactgg
    12361 agcaagtatt ttgaacgacg tttcggttct cagccgttcc ctcctcttct ggcgaagttt
    12421 tacccacttg attggaggga tgggagtgct tgtttttgca cttgctatta tggacaatgc
    12481 caaaaatagc cacctagagg tgatgaaggc tgaggttcca ggtcctgttt ttggcaaggt
    12541 tgtatccaaa ctaaaaaaca ctgcccagat tctctatctc ctttatctag ctctcttctc
    12601 cctctttgtc atcatctatt atctagctgg tatgcctctc tacgatagtt ttgtcattgc
    12661 tatggggaca gcgggaactg gaggctttac cgtctataac gacggaattg cccactatgg
    12721 cagctcactg attacctatc tggtcagtat cggagttctg gtttttgggg taaatttcaa
    12781 cctctactac tacctcatgc tccgtcgcat caaggccttc tttggtgacg aagaacttcg
    12841 ggcttacttg gtcattgtac tggtttctac aggcttgatt agcctcaaca ccctctacct
    12901 ctacccaggg ttttcaaaga gctttgaaat gaccttcttc caggtttcca acatcattac
    12961 aacaactggt tttggatatg gagatattac caactggccc ctcttctccc agtttatcct
    13021 ccttttcctc atggcaatcg gtggctctgc tggatcaact gcaggtggac tcaagattat
    13081 tcgaggcctc atcctttcaa aaattgccaa aaaccaaatt ttgtcaatcc tatcgcccca
    13141 ccgtgttttg actctccatg tgaatcaaac ggtgattgat aaagataccc agcataagat
    13201 tctcaagtac tttgtcatct atgctatgat tttgctagcg cttatcttta ttgtcagcct
    13261 agatagcaat gattttctgg tcgtgaccag cgctgtcttt agctgtttca ataatatcgg
    13321 gcctattcta ggaaccactt ctagtttctc aatctttagt cccatctcaa aaattctcct
    13381 ctcctttgca atgattgcag ggcgcttgga gatttaccca atcctccttc tctttatgaa
    13441 aagaacttgg tctaagagat aaaatacaaa atccctcttt ttacaattgt gaaagaggga
    13501 tttttatatt aaaaaggggg aacgttttta aatccaagcc tgtctgtaag caaagttctt
    13561 actcaaataa aagatggaaa aacttactac acagcttggt aatgctccaa tacaaattgt
    13621 aaggctttac ttggattctt tgttaaatga atatattcca ttcctctggt agcaactatc
    13681 tgaataccta atctttctgc atccaccttc atctctctat aataactccg aacttgtgga
    13741 gccagaataa ttaaatcata aacacccata atatcataat gagctccgta cgctcctgaa
    13801 ttcgcaatca ctctaacttc tgctaattga gcgccctcgt taattgcatt ggctaattac
    13861 gcacttgttc cagaccctgc acaaagaacc agtactttca actccttact ttttattaca
    13921 tctatctcac caggcattat ttcacttgta tcctcctcta aaataatatc atttgagctt
    13981 gcaatatcta caacctgtag tagatatctc gtatttagac aatatgaaaa caagacgact
    14041 tccatatagg aaaccgcctt ctctctatgt tgagtgattt atattaaaat aacttttctt
    14101 ctagctgcat tttattatta taaaaacatt catcataacc ctgaacttaa ataacgattt
    14161 ttattcaag
//
LOCUS       DAVMEI010000039        14068 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVMEI010000039 DAVMEI010000000
VERSION     DAVMEI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.039"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            83..880
                     /locus_tag="VYQ24_000907"
     CDS             83..880
                     /locus_tag="VYQ24_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1968066.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="VYQ24_000908"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="VYQ24_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VYQ24_000909"
     CDS             2383..3657
                     /locus_tag="VYQ24_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1968067.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VYQ24_000910"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VYQ24_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1968068.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4806..5108
                     /locus_tag="VYQ24_000911"
     CDS             4806..5108
                     /locus_tag="VYQ24_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1968069.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5216..5854
                     /gene="udk"
                     /locus_tag="VYQ24_000912"
     CDS             5216..5854
                     /gene="udk"
                     /locus_tag="VYQ24_000912"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1968070.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5982..7322
                     /locus_tag="VYQ24_000913"
     CDS             5982..7322
                     /locus_tag="VYQ24_000913"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1968071.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEKGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLTHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7300..7512
                     /locus_tag="VYQ24_000914"
     CDS             7300..7512
                     /locus_tag="VYQ24_000914"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1968072.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7509..8384
                     /locus_tag="VYQ24_000915"
     CDS             7509..8384
                     /locus_tag="VYQ24_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1968073.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQIIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8377..9192
                     /locus_tag="VYQ24_000916"
     CDS             8377..9192
                     /locus_tag="VYQ24_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1968074.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9185..9616
                     /locus_tag="VYQ24_000917"
     CDS             9185..9616
                     /locus_tag="VYQ24_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1968075.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9623..11290
                     /gene="recN"
                     /locus_tag="VYQ24_000918"
     CDS             9623..11290
                     /gene="recN"
                     /locus_tag="VYQ24_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1968076.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSEIYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11292..12020
                     /locus_tag="VYQ24_000919"
     CDS             11292..12020
                     /locus_tag="VYQ24_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1968077.1"
                     /translation="MIDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12099..13922
                     /gene="lepA"
                     /locus_tag="VYQ24_000920"
     CDS             12099..13922
                     /gene="lepA"
                     /locus_tag="VYQ24_000920"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1968078.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4163 a         2565 c         3352 g         3988 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatgatttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat gatatggagg
     1021 tcaccttatg acttgtgatt ttaaatctga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaata ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgagg gttcaagctg
     1741 ccacaatttg agctatattc gtgatgtggg tgcagcaacc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagtctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa actctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caaccactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaacc aaattcgtct
     2221 gtcaatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattgc agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggctttggag
     2701 aaccaattgg ccactttgcg tttggagcat gaaaatcaac tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gtccgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggccgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagccacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc agttgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat taacttaaaa aaggaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaatcactc tgggctattc cacgacgact
     3901 gaggatgcta gtggggaagt ggtcgcagaa acccctgttt tgtctctctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta ctcagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac gtacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga attggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataata attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct attaggtact
     4681 caaataatct atatagaaat ttagaggtgt gaaatgaagc aatttaaaat tctttcagat
     4741 aaatatttag agtccattac aggttctggt gggaacttag gcccaggatt tggtgtgata
     4801 attccatgat gcgaaatgag tttcgagaaa gggtggagca acttcttcaa caaaaagaaa
     4861 taaatgaaaa tagtgagttg agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa
     4921 atgaaaaata ccaaacggtc atggccaatt tgagtcaagg gttgtcactt tacctcatga
     4981 cgcatcatta ccaggcacct aagtctgtca ttgattttgg tttatggatt gccaaagctc
     5041 ctagccagga aagagggaga ctggctttct tgcagatgct tgctcaaacc ctacagggct
     5101 ttcgttaaaa ctgtcgaaca tcagagggaa gactctggtg ttttttgctg atgagactat
     5161 aaattttgta aaaaatgtga tagaatagac gacggataaa ataacggagg atagcatgca
     5221 aaatagacca atcattattg gagtgacagg tggttctggt ggtggtaaaa ctagtgtttc
     5281 aagagccatt ttatcgcatt ttcctgatga aaagatttcc atgattgagc atgattcata
     5341 ctacaaggat cagtctcatt tgacctttga agagcgtgtc aaaaccaact atgaccatcc
     5401 ttttgccttt gatacagact tgatgatcga gcagattaag gaattgttgg cagggcgtcc
     5461 ggtggacatc ccgacctacg actatacaga gcatacacgg agtagcaaga cctatcgtca
     5521 ggagcctcaa gatgtcttta tcgttgaggg cattttggtc ttggaggaca agcgtctgcg
     5581 cgatttgatg gatatcaaga tttttgtgga tacggatgac gatgtgcgca ttattcgtcg
     5641 tatcaagcgt gatatggagg agcgtggccg tagccttgat agcgttatta accagtactt
     5701 aggtgtggtc aaaccaatgt accaccagtt tatcgagtca actaagcgtt atgctgatat
     5761 cgtcattcct gaaggggtta gcaataccgt ggctatcgac ctgttgacga ccaagattgc
     5821 aaagattttg gaagaagctc gaaacagcaa ataatcacat gtggaggcct tgcctccttt
     5881 ttctattttt ccctcataat ggtacataaa tggagatttt taggcatatt tttggtataa
     5941 taatacccat gaaagagcaa gtaaatgaat agtaggtgga gatggaaaag tatttatcgg
     6001 taacaacttt gaccaagtat ctgaaaatga aattcgataa agacccatac ttggaacggg
     6061 tctatttaac tggtcaagtt tccaactttc gtaaacgacc tactcaccaa tatttctccc
     6121 taaaggatga ccatgcagtt attcaagcga ccatctggtc tgggatttat cagaaattag
     6181 ggtttgacct ggaagaagga atgaagatca atgtgattgg gcgtgtacag gtctatgaac
     6241 caagtggtag ctactccatc atcattgaaa aagctgagcc tgatggggtt ggggcgcttg
     6301 cgattcagtt tgaacaactt aagaaaaaat tgacagaaaa aggcctgttt caagaacgct
     6361 tcaagcaagc tctaccccaa ttttctaaga gaattggggt agtaaccagc cgtagtggag
     6421 ccgttattcg agatattatc acgaccgtca gcaggcgatt cccaggtgtt gacatccttc
     6481 tttatcctac caaggttcaa ggtgaagggg ctgcggagga aattgctaga aatattgcgc
     6541 gtgctaatca acgggacgat ttggatttgc tcattattgg tcgtggaggt ggttctattg
     6601 aggatctctg ggcctttaac gaagaaattg tggtacgagc tatttttgaa tctcgtttgc
     6661 cagttatctc tagtgtgggg catgagacgg atgtgacctt ggcagatttt gtggcagatc
     6721 gacgcgctgc aacgccaaca gcggcggctg aactggcaac acctgtgacc aagttggatg
     6781 tattaactca tttgcaaaat caggaaaaac ggatggcaac ggcagtccga aatgttctat
     6841 ctaagaaaca agaggctttg aaaaaatgca gtcagtctgt tatctttaga caacctgagc
     6901 gcttgtatga cggttatttg caacgcttgg accaactgca actgcgtttg aaacaaagtt
     6961 tgcgaactcg gatttctgat aacaaacaat tagttcaagc aagaactcat caattagtac
     7021 aattatcacc tgttaccaaa atccaacgct atcaagaccg tttaggacag ttggacaagc
     7081 tcttaggtag ccaaatggcg ttagtttatg acgccaaggt tgctgaggcc aagcgacttt
     7141 cggaagcttt gctcatgttg gatactagcc gaatcgtggc gcgtggttat gctattgtca
     7201 aaaaagaaga gtccattgta gattcggttg agagtttgaa gaaaaaagac caagtaacgc
     7261 ttttgatgcg agatggtcaa gtagaattag aggttaaaga tgtcaaaaca aaagaaattt
     7321 gaggaaaatc tagcagaact ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct
     7381 ctggaagatg cgattactgc ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct
     7441 acgctggaca aggctgaaaa gaccttggtc aaggtcatgc aagaagacgg aacagaaagt
     7501 gattttgaat gaaaaagcaa gaaaaattag ctcttgtcga gtcggctttg gaagattttt
     7561 atggagacca gcagtttgcc tctagtttac gggagtctgt tctctattct attcatgctg
     7621 gtggcaagcg tattcggcct tttctcttgt tagaagttct ggaagccttg caggttacca
     7681 tcaaacctgc tcacgcgcag gtagctactg ccttggagat gattcataca gggagcttga
     7741 ttcacgatga ccttcctgct atggatgatg acgattatcg aagagggcgg ttaaccaatc
     7801 acaagaaatt cggtgaagct atggccattt tggctggaga tgccttattc ttagacccat
     7861 atgccttgat tgcgcaggca gatttgccaa gtcagattaa ggtggacttg attgccaact
     7921 tatcccttgc ttcaggtagt ctgggtatgg tggcagggca agttttggat atggagggcg
     7981 aacaccagca cttgtctttg gaagaacttc agattattca tgccaataag actgggaagt
     8041 tactagccta tcccttccaa gcggcagcta ttatagctga attgtcacct gaaatgcagg
     8101 tgaagctgaa aactgtgggt gaattgattg gacttgcttt tcaagtcaga gatgatgtac
     8161 tggatgtgac agctagtttt gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag
     8221 aaaaatcaac ctatcctgcc ctgttgggct tggaagagtc cattgccttt tgtaaccaaa
     8281 ccctggatca agctaatgaa aaattagaag aaattgccca gcagcttccc tttgaaacag
     8341 aatcgattgt aagtgtagta gaaagtttga gaatcaatgg ctaaggaaag agtggatgta
     8401 ctagcttata aacaggggtt gtttgaaacg agagagcagg ccaagcgagg tgtaatggct
     8461 ggcctagtcg tagcagtcct taatggagaa cggtttgaca agccaggaga gaaaattcca
     8521 gatgacaccg aattaaaact caagggagag aaactcaagt atgtcagccg tggtggtttg
     8581 aaactggaaa aggccttgca ggtctttgat ttgtcggtgg atggcgcgac tacgattgat
     8641 atcggggcct ctactggagg ttttaccgat gtcatgctac agaatagtgc caagttggtc
     8701 tttgcagtcg atgttggtac caatcagttg gcttggaaat tacgccaaga cccacgagtt
     8761 gttagcatgg agcagttcaa tttccgctat gctgaaaaga ctgatttcga gcaggagccg
     8821 agctttgcca gtattgatgt gagtttcatt tcccttagtc tgattttgcc agccttgcac
     8881 cgtgtcttgg ctgatcaagg tcaggtggta gcacttgtca aacctcagtt tgaggcagga
     8941 cgtgagcaga ttgggaaaaa tggaattatt cgagatgcta agattcatca gaatgtcctt
     9001 gaatctgtaa cagctatggc agtagaggca ggtttttcag tccttggctt ggacttttct
     9061 cccatccaag gtggacatgg aaatattgaa tttttagcgt atttgaaaaa agaaaagtca
     9121 gcaagcaatc agattcttgc tgagattaaa gaagcagtag agagggcgca tagtcaattt
     9181 aaaaatgaat aaaaaagaaa gacttgaaaa aattagaaga ttggtgacag attatcaaat
     9241 cggcacgcaa gaagaaattg tagaacattt gaaagaagca ggtatcactg ccactcaggc
     9301 gacggtatcc cgagatatca aagagttagg tattgtcaaa attcctttga gagacaacac
     9361 ctatgtctac gaattgccaa aatcaatcgt aaaaagtctg caactggctg aagacaatat
     9421 cgaatcggct gaattgatgg ataagatgat caatctccaa gttattccag gaaatacggc
     9481 ttttgtaaaa gctcagttaa tcgagacttt tgcagacaag atttttagtt gtttgactga
     9541 tgatagctcg attttagtca ttgccagaag tggaagtcta gcagaggaaa tctttgaaca
     9601 agtaaaaaat tggtaggtct atatgttact tgaaatttcg ataaaaaact ttgccattat
     9661 tgaggctatt tccctcaatt ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc
     9721 agggaagtcg attatcattg atgccatgaa tatgatgttg ggagctcgtg cgacgacaga
     9781 tgttattcgt catggtgcac caaaggcaga gattgagggg cttttctcag ttgagaatag
     9841 tcgtctttta caggaaattt ttgatgagca aggtttagaa ttaggtgatg aaattatcat
     9901 ccgtcgggaa atcttgcaaa atggtcgtag tatcagccgt gtaaatggtc agatggttaa
     9961 tctgtctgtt ttgcgagcta ttgggcaaca tcttgtagat attcatggtc agcatgacca
    10021 tgaggagtta atgcgtcccc aactgcatat ccagatgttg gatgaatttg gtgatgccgc
    10081 tttttgggac ttgaaagaaa cctatcaaac gagttttgat gcctatcgga aaatgcgcaa
    10141 gcaggttctg gaagtcaaga aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga
    10201 atttcaaatg gcagagattg aggcagcaaa cttgcaggct ggagaagact tggctctcaa
    10261 tcaagagcga gataaactcc tcaaccacaa aaatattgcg gatacactga ccaatgccta
    10321 cagtatgttg gacaatgaag atttttcaag tctggccaat gttcgttcag ctatgaatga
    10381 catggaaagt gtcgaagagt atgatcctga ataccgtgaa atttcaagct ctctgtctga
    10441 gatctactat gttttagaag acattagcaa acgtttggaa gctatcattg aggaccttga
    10501 ttttgatggc aatcgcctca tgcaggttga gaatcgtttg gacctccttc atactattac
    10561 tcgtaagtat ggtgggactg ttgatgatgt tttgctttat tttgccaaga ttacggaaga
    10621 atacaatctc ttgacaggca ataatctttc gtctgaggac atggaagcag agcttaagaa
    10681 gttggaagtc aatcttgtca atttggcagg tcaacttgct tctgctcgtc ataatttggc
    10741 tcagcaactc gaagctgaga ttaaacaaga actgcaagat ctttatatgg aaaaagccca
    10801 gtttcaggtt cgttttagta agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt
    10861 ttatatttca accaaccctg gagaagactt taaacccttg gttaaggttg cttctggagg
    10921 ggaattatct cgtctcatgt tagccattaa gtctgccttt tcacgtaaag aaggcaagac
    10981 tagcattgtc tttgatgagg tggatacggg agtttcaggt cgtgttgctc aagctattgc
    11041 tcagaaaatt cataaaattg gtcagcatgg tcaggttttg gctatctccc atttgccaca
    11101 agtaattgcg attgcagatt atcaattctt tattgagaag attagtaatg accattcaac
    11161 ggtttcgact gttcgtctct tgacggtcga agagcgagtg gaggaagttg ccaagatgtt
    11221 ggcaggtgat gatgtgacag aagcagccct gacgcaagcc agagaattgt tgagaaacag
    11281 ggagaaataa gatgatagat tattatgtaa ttggagatgt tcacggaaaa gctgggatgc
    11341 tggaagacct tcttaaaacc tgggatggtc agacccagtt gctctttcta ggtgatttga
    11401 ttgacagagg tgaggatagt caccgtgttc ttgaaatggt caaggacttg gtggacaatc
    11461 aaggggctat ctgtttgtct ggaaaccacg agtatatgtt tttgacttgg ctagatgacc
    11521 cagaagaaag ttatgaccat tatcgtcgca atggtggaga tacaaccatt aactctatcc
    11581 taggtcgtcc cttggatgca ccagttgatg gagtagaaga tgccaagcgg gttgccgctg
    11641 aagcggcaga cttggtcgaa ttcattcgtc aaatgccatt tgtagtagag acagacaagt
    11701 atatctttgt tcacgcaggt attgatttga ccttggacga ctggcatgaa acaacagatt
    11761 ataaaaaagt atggcttaga aaaccattcc acggagctga aaatcatact ggaaaaacca
    11821 ttgtcttcgg tcatacaccg gtctatggtt tgttacagca agaacgcggt acatctgagc
    11881 tttggactac agaagatggc aagattggaa tggatggagg agctgtttat ggtggtgtcc
    11941 ttcacgggat tgtctttaca gaccaaggaa tgacagaaca ccactttatc gaaaatgacg
    12001 gctttgtcgc tgaagattag tactcctagc agggtatggt cttgtcaaaa tatcaaaaac
    12061 aatttataat aaatagatac cctgaaagga agagaatcat gaacttagaa gaattgaaaa
    12121 aacgacagga gaagatccgt aacttctcta ttatcgccca tattgaccac ggaaaatcaa
    12181 ctctagcaga ccgcattttg gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc
    12241 agcttttgga tagcatggag ctagagcggg aacgtggaat tactattaag ttgaatgcca
    12301 tcgagttgaa ttacactgca aaagatgggg aaacttatat tttccacttg attgacacac
    12361 cagggcacgt tgactttacc tatgaagttt cacgttcgct agctgcctgt gagggtgcta
    12421 ttttggtggt cgatgcggct caaggaattg aggctcaaac tcttgccaac gtttatctgg
    12481 ctttggataa tgatttggaa atcatgccta tcattaataa aattgacctg ccggctgcag
    12541 atccggagcg cgtgcgtaca gagattgaag atgtgattgg tttggatgcc agtgaagcag
    12601 ttttggcttc tgccaaggct ggtattggga ttgaagaaat cctcgagcaa attgtagaaa
    12661 aagtaccagc accaacgggt gatgtgacgg cgccacttaa ggccttgatt ttcgactctg
    12721 tttacgatgc ttaccgtggg gttatcctcc aagtacgtgt catggacgga gtggtcaaac
    12781 ctggtgataa gattcagctc atgagcaata gtaagacctt tgatgtggcc gaagtcggta
    12841 tttttacacc aaaagcggtt ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg
    12901 cggcgtctat caagacggtt caggatactc gtgtgggtga taccgttacc ttggcaacca
    12961 atcctgcggc agaaccatta catggttata agcagatgaa tcctatggtc tttgcgggtc
    13021 tctacccaat cgaatcaaac aagtacaatg acctgcgtga agcgcttgaa aaattgcaac
    13081 tgaatgatgc tagtcttcag tttgaaccag aaacatctca ggcacttgga tttggtttcc
    13141 gttgtggatt tcttggactt ctccatatgg atgttatcca ggaacgttta gagcgtgagt
    13201 tcaatattga cctcatcatg acagctccgt ctgttattta caaagttaat ttgaccgacg
    13261 gtgagtctat ggatgtgtct aacccatctg agtttccaga cccaactaag attgcgacca
    13321 ttgaagagcc ttatgtcaag gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa
    13381 tggagctagc tcagcgtaag cgtggggact ttgtgactat ggactatatt gatgataacc
    13441 gtgtcaatgt tatctatcaa attcctcttg ctgaaattgt ctttgacttc tttgataaac
    13501 ttaagtcttc aacacgtggt tatgcaagct ttgactacga attgtcagaa tatcgcccat
    13561 ctaagctggt gaaaatggat atccttctca atggagataa ggtggatgct ctcagcttta
    13621 tcgttcacaa ggactttgcc tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa
    13681 tcatccctcg ccaacaattt gaagttccaa tccaagcggc tattggacac aagattgtcg
    13741 ctcgtactga tatcaaggcc cttcgtaaga acgtacttgc taaatgttat ggtggtgacg
    13801 tttctcgtaa gcgtaaactc cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca
    13861 tcggatcagt tgaagttccg caagaagcct tcctcagcgt cttgagtatg gatgaagaat
    13921 aagttatcag aaatgccttt ccttgttcac aggaaattta tataagaatc aatagtaaag
    13981 caggcttaga tggtctgctt ttttagtttt tatactcttc gaaaatctct tcaaaccacg
    14041 tcatcgtcgc cttaccgtat gtatggtt
//
LOCUS       DAVMEI010000040        13992 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVMEI010000040 DAVMEI010000000
VERSION     DAVMEI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.040"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            81..950
                     /locus_tag="VYQ24_000921"
     CDS             81..950
                     /locus_tag="VYQ24_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1968079.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            954..1910
                     /locus_tag="VYQ24_000922"
     CDS             954..1910
                     /locus_tag="VYQ24_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1968080.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1910..2674
                     /locus_tag="VYQ24_000923"
     CDS             1910..2674
                     /locus_tag="VYQ24_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968081.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2674..3384
                     /locus_tag="VYQ24_000924"
     CDS             2674..3384
                     /locus_tag="VYQ24_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968082.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3798..4454
                     /locus_tag="VYQ24_000925"
     CDS             3798..4454
                     /locus_tag="VYQ24_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1968083.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            4562..5657
                     /gene="prfB"
                     /locus_tag="VYQ24_000926"
     CDS             join(4562..4633,4635..5657)
                     /gene="prfB"
                     /locus_tag="VYQ24_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1968084.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5675..6367
                     /gene="ftsE"
                     /locus_tag="VYQ24_000927"
     CDS             5675..6367
                     /gene="ftsE"
                     /locus_tag="VYQ24_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1968085.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6360..7286
                     /locus_tag="VYQ24_000928"
     CDS             6360..7286
                     /locus_tag="VYQ24_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968086.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVETNTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            7572..9752
                     /locus_tag="VYQ24_000929"
     CDS             7572..9752
                     /locus_tag="VYQ24_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1968087.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            9804..10619
                     /locus_tag="VYQ24_000930"
     CDS             9804..10619
                     /locus_tag="VYQ24_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1968088.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            10759..12102
                     /locus_tag="VYQ24_000931"
     CDS             10759..12102
                     /locus_tag="VYQ24_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1968089.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            12317..13507
                     /locus_tag="VYQ24_000932"
     CDS             12317..13507
                     /locus_tag="VYQ24_000932"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1968090.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         4166 a         2482 c         3096 g         4248 t
ORIGIN      
        1 ctttgatttt cattgagtat aagaacctat caaaaagtga gggaaacccc tcggaattat
       61 aaatagaaag agtgaatctt atgctccaac aactcgtaaa tggtttgatt ctaggtagtg
      121 tttacgcgct gttagcccta ggatatacca tggtttacgg aattatcaag ctcatcaact
      181 tcgcccatgg tgatatttat atgatgggag cctttatcgg ttatttcttg atcaattctt
      241 tccaaatgaa tttctttgta gcgcttattg tagctatgct agcgacagct attcttggtg
      301 tcgtgattga gtttcttgct taccgacctt tgcgccactc tactcgtatt gctgttttga
      361 ttacggctat tggggtttct ttcctattgg agtatggaat ggtctatctg gttggtgcca
      421 atacccgtgc cttccctcaa gcgattcaaa cagttcgata tgatttggga ccaattagct
      481 taacaaatgt gcagttaatg attttggcta tttccttgat tttgatgatt ttgttacaag
      541 tcattgtcca aaagactaag atggggaaag ccatgcgtgc agtatcagta gatagcgacg
      601 cggcgcaatt gatggggatc aatgtaaacc gtacgattag ctttaccttc gctttgggtt
      661 ctgctcttgc gggtgcggct ggtgttctga ttgctcttta ttataactct cttgagcctt
      721 tgatgggggt tactccaggt cttaaatctt tcgttgccgc agtacttggt ggtatcggaa
      781 ttattcctgg tgcggctctt ggtggctttg tgattggtct attggaaacc tttgcgactg
      841 cctttgggat gtcagatttc cgtgatgcca ttgtttatgg aatcttgttg ttgatcttga
      901 ttgtccgccc agctggtatc cttggtaaga atgtgaaaga gaaggtgtaa acgatgaagg
      961 aaaatttaaa agttaatatt ctatggttac tccttttgtt agctggctat agcttgatta
     1021 gtgtactggt ttcagtcgga gtacttaatc tattctatgt acagatttta cagcaaattg
     1081 gaattaatat tattttggct gttggtctca acttaatcgt tggtttttca ggacaatttt
     1141 cacttggtca tgctggtttc atggcgattg gtgcctatgc agcagctatt attggttcta
     1201 aattaccaac ctacggtgcc ttctttggag ctatgcttgt aggggctttg ctttcaggag
     1261 cagttgcctt acttgtcggc attccaacct tgcgcttgaa gggggactat cttgcggtag
     1321 caactctggg tgtttctgaa attatccgta tctttatcat caatggtgga agccttacaa
     1381 atggtgcggc aggtatctta gggattccta actttacaac ttggcaaatg gtttacttct
     1441 ttgtcgtgat tacaaccatt gcaaccttga acttcttgcg tagcccaatt ggtcgttcaa
     1501 ccctctctgt tcgtgaagat gaaatcgctg ctgagtcagt tggggttaat acgactaaaa
     1561 ttaaaatcat cgcttttgtc tttggtgcca ttactgcaag tattgctggg tcacttcagg
     1621 caggatttat cgggtctgtt gtaccgaaag attacacctt catcaactca atcaacgttt
     1681 tgattattgt tgtatttggt ggactcggtt ccattacagg tgcgattgtt tcggctattg
     1741 ttctgggaat tttgaatatg cttctccaag atgttgctag tgtgcgtatg attatttacg
     1801 ctttggcctt ggtattggta atgattttca gaccaggtgg actccttgga acatgggaac
     1861 tgagcctatc acgtttcttt aaaaaatcta agaaggagga acaaaactaa tggcattact
     1921 tgaagtaaaa cagttaacca aacattttgg tggtctaaca gctgttggag atgtgactct
     1981 tgaattgaac gaaggggaac tggttggatt aatcggtcca aacggagctg ggaaaaccac
     2041 ccttttcaac cttttgaccg gtgtttatga accaagcgag ggaacagtaa ccctagatgg
     2101 tcaccttttg aatgggaaat caccttataa gattgcctct ttgggacttg gacgtacttt
     2161 ccaaaatatc cgtctcttta aagatttaac agttttagac aatgttttga ttgcttttgg
     2221 aaaccatcac aaacaacatg tttttactag tttcttacgc ttaccagctt tttacaagag
     2281 tgaaaaagaa ttaaaggcta aagctttgga attgttgaaa atctttgatt tagatggtga
     2341 tgcagagact cttgctaaaa atctttccta cggacaacaa cgtcgtttgg aaattgttcg
     2401 tgcccttgct acggaaccta aaattctctt cttagatgaa ccagcagcag gtatgaaccc
     2461 acaggaaaca gccgaattga ctgagttaat tcgtcgtatc aaagatgaat ttaagattac
     2521 aatcatgttg attgaacacg atatgaatct ggtcatggaa gtaacagaac gtatctacgt
     2581 acttgaatat ggccgtttaa ttgctcaagg aactccagac gaaattaaga ccaataaacg
     2641 cgttatcgaa gcttatctag gaggtgaagc ctaatgtcta tgttaaaagt tgaaaatctt
     2701 tctgtgcatt acggtatgat ccaagcagtc cgtgatgtaa gctttgaagt taatgaagga
     2761 gaagttgttt cccttatcgg tgccaacggt gcaggtaaga caactattct tcgcacattg
     2821 tcaggtttgg ttcgaccaag ttcaggaaag attgaatttt taggtcaaga aatccaaaaa
     2881 atgccagctc agaaaattgt ggcaggtggt ctttcacaag ttccagaagg acgccacgtc
     2941 tttcctggct tgactgttat ggaaaatctt gaaatgggag ctttcttaaa gaaaaatcgt
     3001 gaagaaaatc aagctaactt gaagaaggtt ttctcacgct ttcctcgtct tgaagaacgt
     3061 aagaaccaag atgcagctac tctttcagga ggggaacaac aaatgcttgc catgggacgc
     3121 gctcttatgt caacaccaaa acttcttctt ttagatgaac catcaatggg acttgcccca
     3181 atcttcatcc aagagatttt tgatatcatt caagatattc agaagcaagg aacaaccgtc
     3241 ctcttgattg aacaaaatgc caataaagca cttgcaatct ctgaccgagg atatgtactg
     3301 gaaacaggga aaatcgtcct atcaggaaca ggaaaagaac tcgcctcatc agaagaagtc
     3361 agaaaagcat atctaggtgg ctaaaacaat ccagtggatt gttttagtcg gcagatgaag
     3421 attacgaagt aatcatcatt atagtctggg agactagttt aggttgcaga taaagattgc
     3481 gaagcaatca tcaaatccag tggattgttt tagtcggcag atggagatta cgaagtaatc
     3541 atcaatatag tccgggggac ctttttagtc ggtagattga gattgcaaac aaatctgcat
     3601 ctacattgaa agcttaattt ctaataattg aaaaaatcga atgaaaaatt tcttaccttc
     3661 attcacagag ctcgatttca gagctctttt tgctagctta ttcatacttt tctgaatttc
     3721 gaaaaagaaa tgtaagcgtt tgatagattt acaaaaagat tgtataatag ggataagaat
     3781 agaaaaggag aagtctcatg gcagttaaag attttatgac ccgcaaggta gtttatatta
     3841 gtccagatac aacagtatct catgcagcag atttgatgag agagcaaggt ttgcaccgtc
     3901 tgcctgttat cgaaaatgat caattagttg gtttggtgac tgagggaacc attgcacaag
     3961 caagtccatc taaagcaaca agtctttcta tctatgagat gaattatctt ctgaataaga
     4021 caaaagtaaa agatgtcatg attcgcgatg ttgtcactgt ctcaggctat gctagtctag
     4081 aagatgcaac ttatctgatg ttgaaaaata agattagtat tctccctgtc gtagataacc
     4141 atcaagtata cggagttatt actgaccgtg acgttttcca agcctttctt gaaattgcag
     4201 gttatggcga agaagggatt cgtgtacgct ttgttacaga agatgaagtt ggtgttcttg
     4261 gaaaaattgt ttctttgatt gtagaagaaa atttgaatat ctcccataca gtcaatattc
     4321 cgcgtaagga tggtaaggtg attatcgaag tgcaaatcga tggatcaatt gatttaccag
     4381 ccttgaaaga aaaatttgaa gcaaatggta ttcaagtgga agaaatcgct cgcacttcag
     4441 caaaagtctt gtaagaaggg aagcccaaag gcttcttttt tcatgaaaag gggattagag
     4501 aaaaagatgg aaagaaatga taaaatatgc tataatgaaa taatgtaaaa aaggagtatt
     4561 tatggacatt tcagtaattc gtcagaaaat tgacgcaaat cgtgaaaaat tagcttcttt
     4621 cagggggtct ctttgacctc gaagggctag aggaagagat tgccatcttg gaaaacaaga
     4681 tgacagaacc tgatttttgg aacgataata ttgcagccca aaaaacgtcg caagaattaa
     4741 atgaattaaa aaacacttac aataccttcc ataagatgga agagttgcag gatgaagtcg
     4801 aaattttatt ggattttttg gctgaagacg agtcagtgca tgatgaactg gtagcgcagt
     4861 tagccgaact tgataagata atgaccagct acgagatgac tctactcttg tcagaacctt
     4921 atgaccacaa caatgccatc ttggaaatcc atccaggttc tggtggtact gaggcgcagg
     4981 actggggtga tatgttgctt cgtatgtata ctcgttatgg taatgctaaa ggctttaaag
     5041 tggaagtgtt ggattaccaa gcaggtgatg aggctggtat taagtcggta actttatcat
     5101 ttgaagggcc taatgcctat ggtctcctca agtcagaaat gggtgttcac cgcttagtgc
     5161 gaatctcacc atttgactct gccaaacgtc gccatacctc tttcacatct gtagaagtga
     5221 tgccagaatt ggatgatact attgaagtgg aaatccgtga agatgatatc aagatggata
     5281 ccttccgttc aggtggtgcc ggtggacaaa acgtcaataa ggtttcaaca ggtgtacgtt
     5341 taacccacat tccaactgga attgttgtcc aatcaacagt ggatcgtacc cagtatggaa
     5401 atagagatcg tgccatgaag atgttgcagg ctaagctcta tcaaatggag caagagaaga
     5461 aggctgcaga ggtagattct ctcaaaggtg agaaaaagga gatcacttgg ggaagccaaa
     5521 tccgttctta tgtcttcacg ccttatacta tggtaaaaga tcaccgaact agctttgagg
     5581 ttgctcaggt agataaggtt atggatgggg acttagatgg ttttatcgat gcttatctca
     5641 agtggcgaat tagctaagat agaaaggaac tcacatgtca attattgaaa tgagagatgt
     5701 cgttaaaaaa tacgacaacg gaacgactgc tctacgcggt gtttcggtta gcgttcaacc
     5761 gggggaattt gcttacatcg taggaccttc aggagcaggg aagtcaactt ttattcgttc
     5821 tctgtatcgt gaagtaaaaa tcgataaagg aagcctatca gttgctggtt ttaatctggt
     5881 taagatcaaa aagaaagata tcccgcttct acgtcgtagt gttggggttg tcttccagga
     5941 ttataaattg ttaccaaaga aaactgtcta tgaaaatatt gcttacgcta tggaagtaat
     6001 cggggaaaat cgccgtaata tcaaaagacg agtgatggaa gttttggact tggttggatt
     6061 gaagcataag gttcgttctt tcccaaatga actctcaggt ggggagcaac agcggattgc
     6121 gattgcgcgt gcaattgtaa ataatcccaa agtattgata gctgatgagc caacaggaaa
     6181 tctggatccg gataattcat gggaaattat gaatcttttg gaacggatta acctacaagg
     6241 aacaactatt ttgatggcga ctcataatag ccagattgta aataccttgc gccaccgtgt
     6301 cattgccatt gaaaatggcc gtgtcgttcg tgacgaatca aaaggagagt atggatacga
     6361 tgattagtag attttttcgc catttatttg aagccttaaa aagtttgaaa cgaaatggtt
     6421 ggatgacagt agctgctgtc agttcagtca tgattacttt gaccttggtg gcaatatttg
     6481 catctgttat tttcaataca gcgaaactag ctacagatat tgaaaataat gtccgtgtag
     6541 tagtttatat ccgaaaggat gtggaagata atagtcagac aattgaaaaa gaaggtcaaa
     6601 ctgttacaaa taatgactac cacaaggtat atgattcttt gaagaacatg tctacggtta
     6661 aaagtgttac cttttcaagt aaagaagaac aatatgaaaa attaaccgag ataatgggag
     6721 ataactggaa aatctttgaa ggagatgcca atcctctcta tgatgcctat attgtagaga
     6781 caaacactcc aaatgatgta aaaactatag ccgaagatgc taaaaaaatt gaaggtgtct
     6841 ctgaggttca agatggcggt gccaatacag aaagactctt caagttagct tcatttatcc
     6901 gtgtttgggg actagggatt gctgctttgt taatttttat cgcagttttc ttgatttcaa
     6961 ataccattcg tattaccatt atttcccgca gtcgcgaaat tcaaatcatg cgcttggtcg
     7021 gagctaaaaa cagttatatc cgtggaccgt tcttgttaga aggagccttt atcggtttat
     7081 tgggagctat cgcaccatct gttttggtct ttattgttta tcaaattgtt taccaatctg
     7141 tcaacaaatc gttggtaggg caaaatctat ccatgattag tccagattta tttagtccgt
     7201 tgatgattgc cctactattt gtgattgggg ttttcattgg ttcattggga tcaggaatat
     7261 ccatgcgccg attcttgaag gtttaggtaa aatagctgct tttatgagga gattgtaaaa
     7321 tctccttttt tgctacaaga gtttttgaaa agagatgcgc agaagaaaag agcttccaaa
     7381 gaagtccccc agagaagact tcttgatggc taatttgaac ttgaaatttg gcgcactaga
     7441 aattttttag aaaaaagtgt aattttttta taaaagcctt tacaaaaaaa attaaagtgg
     7501 tataattaaa tcatagaaag tgcaaacgtt ttcgtaaaac gtttgcctaa ataaaaataa
     7561 aggagatttg aatgatgaaa gatacattca aaaatgtctt gtctttcgaa ttttggcaaa
     7621 aattcggtaa ggctttgatg gtagttatcg cggttatgcc ggctgctggt ttgatgattt
     7681 caatcggtaa gtctatcgtg atgattaacc caacctttgc accacttgtc atcacaggtg
     7741 gaattcttga gcaaatcggt tggggggtta tcggtaacct tcacattttg tttgccctag
     7801 ccattggagg aagctgggct aaagaacgtg ctggtggtgc tttcgccgct ggtcttgctt
     7861 tcatcttgat taaccgtatc actggtacaa tctttggtgt atcaggcgat atgttgaaaa
     7921 atccagatgc tatggtaact actttctttg gtggttcaat caaagttgct gattacttta
     7981 tcagtgttct tgaagctcca gccttgaaca tgggggtatt cgtagggatt atctcaggtt
     8041 ttgtaggggc aactgcttac aacaaatact acaacttccg taaacttcct gatgcacttt
     8101 cattcttcaa cgggaaacgt ttcgtaccat ttgtagttat tcttcgttca gcaatcgctg
     8161 caattctact tgctgctttc tggccagtag ttcaaacagg tatcaataac ttcggtatct
     8221 ggattgccaa ctcacaagaa actgctccaa ttcttgcacc attcttgtat ggtactttgg
     8281 aacgtttgct cttgccattt ggtcttcacc acatgttgac tatcccaatg aactacacag
     8341 ctcttggtgg tacttatgac attttaactg gtgcagctaa aggtactcaa gtattcggtc
     8401 aagacccact atggcttgca tgggtaacag accttgtaaa ccttaaaggt actgatgcta
     8461 gtcaatatca acacttgtta gatacagtac atccagctcg tttcaaagtt ggacaaatga
     8521 tcggttcatt cggtatcttg atgggtgtga ttgttgctat ctaccgtaat gttgatgctg
     8581 acaagaaaca taaatacaaa ggtatgatga ttgcaacagc tcttgcaaca ttcttgacag
     8641 gggttactga accaatcgaa tacatgttca tgttcatcgc aacacctatg tatcttgttt
     8701 actcacttgt tcaaggtgct gccttcgcta tggctgacgt cgtaaaccta cgtatgcact
     8761 cattcggttc aatcgagttc ttgactcgta cacctattgc aatcagtgct ggtattggta
     8821 tggatatcgt taacttcgtt tgggtaactg ttctctttgc tgtaatcatg tactttatcg
     8881 caaacttcat gattcaaaaa ttcaactacg caactccagg gcgcaacgga aactacgaaa
     8941 ctgctgaagg ttcagaagaa accagcagcg aagtgaaagt tgcagcaggc tctcaagctg
     9001 taaacattat caaccttctt ggtggacgtg taaacatcgt tgatgttgat gcatgtatga
     9061 ctcgtcttcg tgtaactgtt aaagatgcag ataaagtagg aaatgcagag caatggaaag
     9121 cagaaggagc tatgggtctt gtgatgaaag gacaaggggt tcaagctatc tacggtccaa
     9181 aagctgacat tttgaaatct gatatccaag atatccttga ttcaggtgaa atcattcctg
     9241 aaactcttcc aagccaaatg actgaagcac aacaaaacac tgttcacttc aaagatctta
     9301 ctgaggaagt ttactcagta gcagacggtc aagttgttgc tttggaacaa gtaaaggatc
     9361 cagtatttgc tcaaaaaatg atgggtgatg gatttgcagt agaacctgca aatggaaaca
     9421 ttgtatctcc agtttcaggt actgtgtcaa gcatcttccc aacaaaacat gcttttggta
     9481 ttgtgacgga agcaggtctt gaagtattgg ttcacattgg tttggacaca gtaagtcttg
     9541 aaggtaaacc atttacagtt catgttgctg aaggacaaaa agttgcagca ggagatctcc
     9601 ttgtcacagc tgacttggat gctatccgtg cagcaggacg tgaaacttca acagtagttg
     9661 tcttcacaaa tggtgatgca attaaatcag ttaaattaga aaaaacaggt tctcttgcag
     9721 ctaaaacagc agttgctaaa gtagaattgt aatatacttg aggttggaag ctgtattcca
     9781 acctcttatt ttgggagaaa agaatgaaat ttttaacact caatactcac agttggatgg
     9841 agaaagaagc agaggaaaaa ttccagattt tgcttgaaga tattcttgaa aaggactatg
     9901 atttgatttg tttccaagaa atcaatcagg agatgacctc gtcagaggtg gaggttaatg
     9961 acctttatca agctttgcca gcagctgagc ctattcacca agaccattat gttagactct
    10021 tggttgaaaa gttgttagag caagggaaaa attactactg gacctgggct tataaccata
    10081 tcggctatga ccgctaccac gaaggtgtgg ctatcttgtc taaaacacct attgaagcca
    10141 gagaaatttt ggtttcagat gtggatgatc caacagacta tcatactcgc cgtgttgccc
    10201 tagctgaaac tgtagtcgat ggcaaggagc tagcagttgc cagtgttcat ctctcttggt
    10261 gggataaagg tttccaagaa gaatgggcac gatttgaggc tgtcttgaaa aaattgaaca
    10321 agccactttt actagctgga gattttaaca acccagcagg tcaggaaggt taccaagcta
    10381 ttttagctag tccattaggc ttacaagacg catttgaagt tgctcaagag aaaagtggta
    10441 gctatactgt tccgcctgaa attgatggct ggaaagggaa cactgaaccc cttcgaatcg
    10501 attatgtctt tactaccaaa gagttagcgg tggaaaattt acatgtcgta tttgatggta
    10561 acaagagtcc acaagtgagt gatcactatg gcttgaatgc tatattaaac tggaaataat
    10621 aactgaaaag aggttggaac tataaaattc cagccttttc ttactagaga agctactgga
    10681 aatagcctaa ataagtgaga ctactgtaat ggaataaaat atggtataat tgataagata
    10741 gatagaatcg aggatgttat gtcatttacg aaatttcaat ttaaaaacta tattagagaa
    10801 gccttgaagg agttaaaatt tacaactcca acagaggtgc aagacaagtt gattcctatt
    10861 gttttggcag gtcgtgacct agtaggagaa tcaaaaacag gttcaggtaa gactcatact
    10921 ttcttgttac cgattttcca gcaattagat gaagctagcg atagtgtaca agcagtgatt
    10981 actgcaccga gtcgtgagtt ggctactcaa atttaccaag tagcgcgtca gatttcagct
    11041 cactcagatg tcgaagttcg tgtggttaat tatgtgggtg gtacggataa ggctcgccag
    11101 attgagaaat tggaaagcaa tcagcctcat attgttattg gaacaccagg ccgtatctac
    11161 gacttggtta aatctggtga tttagctatt cataaagcca agacatttgt tgttgatgaa
    11221 gcagatatga ccttggatat gggattcttg gaaactgttg ataagattgc tggcagtctt
    11281 ccaaaagact tgcaattcat ggtcttctca gcgactatcc cacaaaaact gcaaccattc
    11341 ttgaaaaaat acttatcaaa tcctgttatg gagaaaatta agaccaaaac ggttatttct
    11401 gacaccattg ataattggtt gatttcgacc aagggacatg ataagaatgc tcaaatttac
    11461 cagttgactc agttgatgca gccgtatttg gcaatgattt ttgttaacac taaaacgcgt
    11521 gctgatgaat tgcattcata tctgactgct caaggcttga aggttgcaaa aatccatgga
    11581 gatattgccc ctcgtgaacg caagcgaatc atgaatcagg tgcaaaatct ggattttgag
    11641 tatattgtcg caacagattt agcagcgcgt gggattgaca ttgaaggtgt cagccatgtc
    11701 atcaatgatg ccattccgca agacttatct ttttttgttc atcgtgttgg tcgtactgga
    11761 cgaaatggcc taccaggtac agctattacc ctttatcagc caagtgatga ctcggatatc
    11821 cgtgagttgg agaaattggg aatcaagttt agtcctaaga tggtcaaaga cggggaattt
    11881 caagatacct atgaccgtga tcgtcgtgcc aaccgtgaga aaaaacaaga taaacttgat
    11941 atcgaaatga ttggtttggt taaaaagaaa aagaaaaaag tcaaaccggg ttataagaag
    12001 aaaattcaat gggcggttga tgaaaagcgc cgtaaaacca agcgtgctga aaatcgcgct
    12061 cgcggtcgtg cagagcgtaa agctaaacgc caaacatttt aatagaaatt gttggagtat
    12121 tgagctccaa cttttttatt tatgagaacg aactatctaa accgaaacac tacattaaag
    12181 actgcaaatt gcgattaaaa atggtataat gataaagtta tatagtcccg ataagatggt
    12241 aggtatttat tacgaagagt tttcctatca gtactttgta actctataac aatatttttt
    12301 aaggggggac atttttatgt cagaacgtaa attattcacg tctgaatctg tatctgaggg
    12361 gcatccggat aagattgcag accaaatttc agatgcgatt ttggatgcta ttttagcaaa
    12421 ggatccagag gcgcacgttg ctgctgaaac agctgtatat actggttctg tccacgtttt
    12481 tggtgaaatt tctacaaatg cctatgtgga tattaaccgt gtggttcgtg ataccattgc
    12541 agagattggt tataccaata cagaatatgg attttctgct gagacggtgg gagtacaccc
    12601 atctttggtg gaacaatctc ctgacatcgc tcaaggtgtt aacgaagcct tggaggttcg
    12661 tggaaatgct gatcaagatc cactggactt gattggagca ggtgaccaag ggctcatgtt
    12721 tggatttgca gtagatgaaa cagaagagct catgccattg ccaattgcac tcagtcataa
    12781 attggttcgt cgtctggcag aacttcgtaa gtctggagaa attagctatc tccgtccaga
    12841 tgcaaaatca caagttacag ttgagtacga tgaaaatgac cgtccggtac gtgtagatac
    12901 agtcgttatt tctactcagc atgatccaga ggccactaat gaacaaatcc atcaagatgt
    12961 gattaacaag gtcatcaaag aagttattcc atcttcttat cttgatgata agacaaaatt
    13021 ctttatcaat ccgacaggtc gttttgtaat cggtggtcct caaggggact caggtttgac
    13081 tggtcgtaag attattgtag atacttatgg tggctactct cgtcatggtg gtggtgcctt
    13141 ctctggtaaa gatgcgacta aggtggatcg ttcagcctct tatgcggctc gctatattgc
    13201 caagaatatc gttgcagcag gccttgctaa gaaggcagaa gtgcagttgg cctatgctat
    13261 cggtgttgcg caacctgttt ctgttcgtat cgatactttc ggtacaggaa cagtagctga
    13321 aagtcaactt gaaaaagcgg ctcgtcaaat ctttgacctt cgccctgcag ggattatcca
    13381 aatgctggac ctcaagcgtc caatttaccg tcaaacatcg gcttacggtc acatgggacg
    13441 tacagatatt gatcttccat gggaacgttt ggataaggta gatgctttga aagaagcagt
    13501 aaaataagat tttaagaggg gaacgtcctc tcttttttat agtttttaac tatactggga
    13561 tactgttctg aaaatccatt ttgcgaaagt agagatttac atgtatagta gattgaaact
    13621 agaatagtac acctcaactt ctaaaacatt gttagcaatc aatttgactg tcctgatcga
    13681 tttctcctgt tcttgtttca ttttactata tttctttaaa aatgataaag gttgagattt
    13741 ctcctcgtaa tagataatca tggggatatt tcaatccaaa gttttattcg ttatcacttg
    13801 actattgcaa ggttttctag agcaacagag tcatggaatg gactcatggt tgagatttct
    13861 ccttgttgct tggacttcat tcaaaagtct gttacccaag ccttgttcaa acttccaata
    13921 cactagctgt tttcatagca tgacttctgt actagacttt cttttccgaa taaatagata
    13981 gaaccacaga at
//
LOCUS       DAVMEI010000041        13397 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVMEI010000041 DAVMEI010000000
VERSION     DAVMEI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.041"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            148..804
                     /locus_tag="VYQ24_000933"
     CDS             148..804
                     /locus_tag="VYQ24_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1968091.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            794..1117
                     /locus_tag="VYQ24_000934"
     CDS             794..1117
                     /locus_tag="VYQ24_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1968092.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1221..2051
                     /locus_tag="VYQ24_000935"
     CDS             1221..2051
                     /locus_tag="VYQ24_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1968093.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2205..3059
                     /locus_tag="VYQ24_000936"
     CDS             2205..3059
                     /locus_tag="VYQ24_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1968094.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3159..4532
                     /locus_tag="VYQ24_000937"
     CDS             3159..4532
                     /locus_tag="VYQ24_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1968095.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4525..5586
                     /locus_tag="VYQ24_000938"
     CDS             4525..5586
                     /locus_tag="VYQ24_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1968096.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMAKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5588..6280
                     /locus_tag="VYQ24_000939"
     CDS             5588..6280
                     /locus_tag="VYQ24_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968097.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6310..6879)
                     /locus_tag="VYQ24_000940"
     CDS             complement(6310..6879)
                     /locus_tag="VYQ24_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1968098.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPTPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7014..7628
                     /locus_tag="VYQ24_000941"
     CDS             7014..7628
                     /locus_tag="VYQ24_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1968099.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7630..9276
                     /locus_tag="VYQ24_000942"
     CDS             7630..9276
                     /locus_tag="VYQ24_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1968100.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9288..10574
                     /locus_tag="VYQ24_000943"
     CDS             9288..10574
                     /locus_tag="VYQ24_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1968101.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10626..11159
                     /locus_tag="VYQ24_000944"
     CDS             10626..11159
                     /locus_tag="VYQ24_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968102.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11237..11707
                     /gene="nrdI"
                     /locus_tag="VYQ24_000945"
     CDS             11237..11707
                     /gene="nrdI"
                     /locus_tag="VYQ24_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1968103.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12000..13262)
                     /locus_tag="VYQ24_000946"
     CDS             complement(12000..13262)
                     /locus_tag="VYQ24_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1968104.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3922 a         2515 c         3082 g         3878 t
ORIGIN      
        1 tcagttctat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
       61 tgctctttga ttttcattga gtattattaa gacaatatga ggagaaagat gaaagaaaaa
      121 ggattttagg agggggcgca ggcagccatg ccaacggctc ttggctatgt cagtatcggc
      181 ctggcctgtg ggactatcgg tgcgccctat gtgacacctg ttgagatggg cttgatgagt
      241 ctctttgttt atgctgggag tgcccagttt gccatgttgg cactgattgt ggttcaagct
      301 cctgtggcag ctattgctat gacggttttt ctaatcaact tgcgtctctt tttgttgagt
      361 ttacacgcat cgacttattt ccgtcatacc agtctttggt acaatatcgg tatgtctagt
      421 atcttgacag atgagaccta tggcgttttg atgggtgaat tggcccatac agacaaggta
      481 aatcctatgt ggatgcacgg aaacaatctt aacagctatg tggcttggtt tgtggggaca
      541 gtagtcggaa cggctctggg tggcctgcta ccaaatccag aaatctttgg cttggatttt
      601 gccctggttg ggatgtttat tggtattttt gcttcgcaat ttcagattat gcaaagacgg
      661 attcctgtcc gcaatctgct cattatccta gcagttgttg cggtgtcctt ctttttgctc
      721 ttgacagtgg tgtctcagtc actagctgtt ctgtttgcga cgctacttgg ttgtagcatg
      781 ggggtggttt tagatggtca gtaagtatct tttattagca gttattttct ctggcttggt
      841 gacttggatt ctccgtatga ttcccttcat cttagtcaag tataagggct tgcctgcaat
      901 cgttgagcgt tttttgaagt tcttgcccgt ttccattatc tttgccttga ttctttcaag
      961 cgtagtgaca ggtaaggttg ggagccttcc tcaaattaaa tggctagact tcttagccgt
     1021 ctttccaaca gcttgggtag cctttcgcta ccgcaatcta gtcggaacag ttctctttgg
     1081 agtggtcttg attgccatct tgcgtttggt cttttaaatt acccataaaa aaaacttatc
     1141 acagagatag atatcatata atggcgtaaa tgctcttttt ctgttaagat tataaggtat
     1201 tctattttgg aggaaatgac atgaaaaaaa tcgttaaata ctcatctctt gctgccctag
     1261 gacttgttgc tgcaggtgtg cttgcggctt gctcaggggg tgctaagaaa gaaggagaag
     1321 cagctagcaa gaaagaaatc atcgttgcaa ccaatggatc accaaggcca tttatctatg
     1381 aagaaaatgg cgaattgact ggttacgaga ttgaagtcgt tcgcgctatc tttaaagatt
     1441 ctgacaaata tgatgtcaag tttgaaaaga cagaatggtc aggtgtcttt gctggtcttg
     1501 atgctgaccg ttacaatatg gctgtcaaca atcttagcta cactaaagaa cgtgcggaga
     1561 aatacctcta tgccgcacca attgcccaaa atcctaatgt ccttgtcgtg aagaaagatg
     1621 actctagtat caagtctctc gatgatatcg gtggaaaatc gacggaagtc gttcaagcca
     1681 ctacatcagc taagcagtta gaagcataca acgctgaaca cacggacaac ccaactatcc
     1741 ttaactatac taaggcagat ttccaacaaa tcatggtacg tttgagcgat ggacaatttg
     1801 actataagat ttttgataaa atcggtgttg aaacagttat caagaaccaa ggtttggaca
     1861 acttgaaagt tatcgaactt ccaagcgacc aacaaccgta cgtttaccca cttcttgctc
     1921 aaggtcaaga tgagttgaaa tcatttgtag acaaacgcat taaagaactc tacaaagacg
     1981 gaacgcttga aaaattgtct aaacaattct tcggagatac ttatctacca gcagaggctg
     2041 atattaaata atttcttgaa atcatccatt ctgagtaaga tgggtgattt ctcagttttt
     2101 agggatatag ttttaaacta tatatctgat tatctaataa caatttgtga aatcaaacaa
     2161 attgtgagat actagtacgg tattattttt aaggagaaag aatcatgaaa atcaaaaaat
     2221 ggcttggcct agtagccctt gctacagtcg caggtttggc tcttgcagct tgcggaaact
     2281 cagaaaagaa agcagacaat gcaacaacta tcaaaatcgc aactgttaac cgtagcggtt
     2341 ctgaagaaaa acgttgggac aaaatccaag aattggttaa aaaagacgga attaccttgg
     2401 aatttacaga gttcacagac tactcacaac caaacaaagc aactgctgat ggcgaagtag
     2461 atttgaacgc tttccaacac tataacttct tgaacaactg gaacaaagaa aacggaaaag
     2521 accttgtagc gattgcagat acttacatct ctccaatccg cctttactca ggtttgaatg
     2581 gaagtgccaa caagtacact aaagtagaag acatcccagc aaacggagaa atcgctgtac
     2641 cgaatgacgc tacaaacgaa agccgtgcgc tttacttgct tcaatcagct ggcttgatta
     2701 aattggatgt ttctggaact gctcttgcaa cagttgccaa catcaaagaa aatccaaaga
     2761 acttgaaaat cactgaattg gacgctagcc aaacagctcg ttcattgtca tcagttgacg
     2821 ctgccgttgt aaacaatacc ttcgttacag aagcaaaatt ggactacaag aaagcacttt
     2881 tcaaagaaca agctgatgaa aactcaaaac aatggtacaa catcattgtt gcgaaaaaag
     2941 attgggaaac atcacctaag gctgatgcta tcaagaaagt aatcgcagct taccacacag
     3001 atgacgtgaa aaaagttatc gaagaatcat cagacggttt ggatcaacca gtttggtaat
     3061 aagaaacagg gaggtgggag agagaattcc acctcttgct tttgtataga gcacggagtg
     3121 taaagaagac tattcgttca tagaaaggta gagagaatat ggtttttcct agtgaacaag
     3181 aacagattga aaaatttgaa aaggatcatg tagcccagca ttattttgag gttttgcgta
     3241 ccttgatttc taagaaatca gtctttgccc agcaggttgg actcaaggaa gtcgcaaatt
     3301 atctgggtga gattttcaag cgtgttggag ctgaagtgga gattgatgag agctatacag
     3361 cgccctttgt catggcacat ttcaagagtt cgcgtccaga tgccaagacc ttgattttct
     3421 ataaccacta tgacactgtg ccagcggatg gggatcaggt ctggacagag gatcctttta
     3481 cgctttcggt ccgcaatggc ttcatgtatg ggcgtggagt tgatgacgac aagggtcata
     3541 tcacagctcg cttgagtgct ttgagaaaat atatgcagca ccatgatgat ttacctgtca
     3601 atatcagctt tatcatggag ggagcggagg aatcggcttc aacagaccta gataagtatt
     3661 tggaaaagca tgcagacaaa ctccgtgggg cggatttgtt ggtctgggaa caagggacca
     3721 aaaatgcctt ggaacagctg gaaatttctg gtggcaataa ggggattgtg acctttgatg
     3781 ccaaggtaaa aagcgctgat gtggatatcc actcgagtta tggtggtgtt gtggaatcag
     3841 ctccttggta tctcctccaa gccttacagt ctcttcgtgc tgcggatggc cgtatcttgg
     3901 ttgaaggctt gtacgaagaa gtacaagagc ccaatgaacg agaaatggcc ttgctagaaa
     3961 cttatggtca acgaaaccca gaggaagtta gtcggattta tggattggag ttgcctctct
     4021 tacaggagga gcggatggcc tttctaaaac gtttcttttt cgagccagcg cttaatatcg
     4081 aaggaatcca gtctggttat caaggtcagg gtgttaagac tattttacct gcagaagcca
     4141 gtgccaagct agaggttcgt ctggttccgg gcctagaacc gcatgatgtt ctggaaaaaa
     4201 ttcggaaaca gctagacaaa aatggctttg ataaggtaga attatactat accttgggag
     4261 agatgagcta tcgaagcgat atgagcgcac cagccattct caatgtgatc gagttggcca
     4321 agaaattcta tccacagggc gtttcagtct tgccgacgac agcggggaca ggacctatgc
     4381 atacggtctt tgatgcccta gaggtaccaa tggttgcatt cggtctagga aatgccaata
     4441 gccgagacca cggtggagat gaaaatgtgc gaatcgctga ttattacacc catatcgaat
     4501 tagtagagga gctgattaga agctatgagt agagatatta tcaagttaga tcagatcgat
     4561 gtgacttttc accaaaagaa gagaaccatt acagcggtta aggatgtgac cattcacatc
     4621 caagaagggg atatctacgg aatcgttgga tattctggag cagggaaatc aacccttgta
     4681 cgggtgatta acctcttgca aaaaccatct gcagggaaaa ttaccattga cgacgatgtg
     4741 atttttgacg gcaaggtgac cttgacggca gagcagttgc gtcgtaaacg tcaagatatc
     4801 gggatgattt tccagcattt taacctgatg agccaaaaga cagcagagga gaatgtagcc
     4861 tttgccctta aacactctgg actcagcaag gaagaaaaga aggctaaagt agctaagttg
     4921 ttggacttgg ttggtttggc agatcgtgct gaaaactacc cttcacaact atctggaggg
     4981 caaaaacagc gtgtggcaat tgcgcgtgcc ttggccaatg atccaaaaat cttgatttca
     5041 gacgagtcaa cttctgccct tgaccctaag acaaccaagc agattttggc cttgttacaa
     5101 gatttgaacc aaaaattagg attgacagtt gtcttgatta cgcatgaaat gcagattgtc
     5161 aaagacattg ccaaccgtgt ggcggttatg caggatgggc atttgattga agagggaagt
     5221 gtccttgaaa tcttctcaaa ccctaaacaa cctttgactc aagactttat ctcaacagcc
     5281 acaggtattg acgaagccat ggccaaaatc gagaagcaag aaatcgtgga acacttgtct
     5341 gaaaacagtc tcttggtgca actcaagtac gctggagctt caacagacga gccacttttg
     5401 aatgaattgt acaagcatta ccaagtaatg gctaatattc tctatgggaa tatcgaaatt
     5461 ctcgatggta ctcctgttgg agaattggtg gtggtcttgt caggtgaaaa agcagcgttg
     5521 gcaggtgccc aagaagctat tcgccaagca ggtgtacaac taaaagtatt gaagggagta
     5581 cagtaagatg gaatcattga ttcaaaccta tttaccaaat gtctataaaa tgggttgggc
     5641 tggtcaggca ggctggggaa cggctatcta cttaactctt tatatgacag ttctttcctt
     5701 cattatcgga ggcttcttgg ggctagtggc aggtctcttt ctcgtcttga cagcgccagg
     5761 tggtgtcttg gagaataaag tcgtattctg gattttagac aaaattacct caatttttcg
     5821 tgcggttccc tttatcatcc tcttggcaat cttgtcacca ctttctcact tgattgttaa
     5881 aacaagtatc gggccaaatg cagcccttgt cccactttct tttgcagtct ttgccttctt
     5941 tgcccgtcag gtgcaggttg tcttggctga attggatggc ggtgtcattg aggcggctca
     6001 agcgagcgga gcgactttct gggacatcgt gggtgtttac ctatcagaag gtcttccaga
     6061 tttgatccgt gtgacgactg tgaccttgat ttcccttgtt ggggaaacag ctatggccgg
     6121 tgcggttgga gctggtggta tcggtaacgt agccatcgct tatggattta accgctacaa
     6181 tcacgatgtg accatcttgg caaccatcat tatcattttg attatctttg caatccaatt
     6241 cttgggagat ttcttgacta agaaattgag ccataaataa aaaagagccg cgtggctctt
     6301 tttaactgat cagattttct gtgcaaattt tttactcaag gcttgtccaa tcaaggcacc
     6361 cactagggct ccgatgacaa tacttgcgat aaatagaagg acagttccag gggttggagc
     6421 gaccatgata cggtcgatat attcttggga ttttcctctt gccagaagag tagccatata
     6481 ggctttgggc gcaatccaca taagcaagat tggtcctgtt gtactaaagg cgaaaataat
     6541 gaaagaaagg aagttctttg ttttgtcctt gtattttcct aaatgagcta ctccatctgc
     6601 taggaggcca cagataattc caggaaggaa ggaaccagca ccgtgtttag ttcccaagaa
     6661 aaagagggca atgacaaggc cgatagtggt aatggctcca aagcgcggaa cttttgcgac
     6721 taggatcata tagacgctac cgccgacaag ggcagtaaag gcaggcgcat aaaacatgtt
     6781 tccagtttgg tcaaagagat tgcccaaaag gacaccaatc cccatgcaga ggaagtaaag
     6841 aactgcaaaa agcagtgtag ttaagataga tttcttcatg aattgtctcc tgataatttt
     6901 ttcacaattc tcattttacc atggtttggt gggattgtaa atggatacta gaagtttttg
     6961 aaaacgcttg aaatatgata taatggtttc atagttattt ttaggggaat atcatgggta
     7021 gatttttaga ctttgtcttt aatcgtttct ttttagggat gattgcgaca gccttctttt
     7081 ggctattaac tttagcagga gggattatcc ttggtctagc gccggctagt gccaccttga
     7141 tgagcttata tgcagaacat ggttatagct ttcgggaata cagtttgaag gaggcttggt
     7201 ctctttacaa gcaaaatttt gtctcaagca acctgatttt ctatagcttt ttaggtgtgg
     7261 gtctagtttt gacctatggt ttgtatctct tggtgcaatt gcctcatcag accattgttc
     7321 atttgattgc gacccttttg aatgtcctag tagttgccct gatctttttg gcttatacag
     7381 tatctttaaa attacaagtt tattttgcct tgtcctatcg aaatagtctc aaattatcct
     7441 tgattggcat ctttatgagt ctagcagctg tggccaaggt tctccttggg actgtgctac
     7501 ttgtagcaat tggttactat atgcctgccc tgctattttt tgtaggaatt gggatgtggc
     7561 atttctttat cagtgatatg ttggaacctg tctatgaaat catccatgaa aaattggcga
     7621 caaaatagaa tgaagcagtt ttggctacat acgcttctaa gaacctatag ttcagtgatg
     7681 atcattatca ttgcgagttt tgcaatctta ctctcttacg ctgactggga ttcacgtgaa
     7741 aaggaagccc agagagtagc ccagcgtgta actgctagaa cagtgagtga aattgaatat
     7801 taccatagag agtcaaccca gatagctcag gctttagttg aaaatcaagc tcgtattgag
     7861 ggaatctata aatactttag ccttagcatg ccagactatt tttactggca attagagcgg
     7921 aaagcttcgc cttatatatc agtctctctg tatgaaaatg ttgatgacct ctatgttcga
     7981 aatgattttg taactggagt ggccattgct tttcaagatt acaaggaagt ctatgtttct
     8041 actaaagaca aacgtagtgg agaaaaaatc agggctgagg atttcaaacc agcaggaaat
     8101 agttttgcca ttccagtgtc agatccagtg tcagatcaag atttaggagt gatttacatc
     8161 tccttggatc ctgctgtttt ataccatgcc attgataata ctagaggtca tactccgatg
     8221 gcagtaacag tgacctcacc ttttgatacg gagatttttc atatgggtga gacagttgat
     8281 aaggagagtg aaaattggct agttggctta acttctcatg gttatcaggt tcaggtggca
     8341 gttcccaaaa actttgtttt acaaggaacg gtgaccagct ctgctttgat tgtgggcttg
     8401 agccttctct ttattgtcat tctttatctg actttgaggc agacctttgc taattatcaa
     8461 aagcaggtag tggatttggt ggattccatc caagctattg cccaaggaca agaaggtctt
     8521 cgcattgata cgcttgaaaa ggatcaggaa ttgctcctaa tcgcggagac gaccaatgat
     8581 atgttggatc gattggaaaa gaatatccat gatatttacc agttagaact cagtcaaaaa
     8641 gatgccaata tgcgggcctt gcaggcgcaa atcaaccctc acttcatgta taatacgctg
     8701 gagttcttgc gtatgtacgc agtcatgcag agtcaagatg agttggcaga tatcatttat
     8761 gaattcagta gtctcttgcg taacaatatt tccgacgaaa gagagaccct cctcaaacag
     8821 gaattagaat tttgccgtaa atacagctat ctctgcatgg ttcgctatcc caagtccatt
     8881 gcctatggtt tcaagataga tccagagtta gagaatatga agattcccaa gtttaccttg
     8941 caaccgctgg tagaaaacta tttcgcgcat ggtgttgacc acaggcggac agataatgtg
     9001 attagcatca aggctcttaa acaggatggg tttgtggaaa ttttggtggt cgataatggt
     9061 agaggaatgt cggctgaaaa gttggcaaat atccgagaaa aattaagtca gaaatatttt
     9121 gaacaccaag ccagctacag tgatcaaagg cagtctatcg ggattgtcaa tgtacacgag
     9181 cgttttgtgc tctattttgg agaccgctat gccattacta tagagtctgc agagcaagcc
     9241 ggtgttcagt atcgtattac aattcaagat gagtagaaag ggagaaaatg tataaagtat
     9301 tattagtaga tgatgagtac atggtgacag aaggtctgaa gcgtttgatt ccctttgata
     9361 agtgggatat ggaggtcgtc gcaacagcca gtcatgccga tgaagctcta gaatatgttc
     9421 aggaaaatcc tgtcgatgtc atcatttccg atgtcaatat gccagacaaa acagggcttg
     9481 atatgattcg ggagatgaaa gagatcttac cagatgctgc ctatatcctg ctctcaggtt
     9541 atcaggagtt tgattatgta aaaagagcaa tgaaccttag tgtggtggac tatttggtca
     9601 agcctgttga taaggtagag ctgggaaatc tgctggagaa gattgcaggt cagctcggcg
     9661 agagagggaa gaaaagtcag actcttagtc aagaattaga cgaggctgga tttgttagtt
     9721 atttagggga taaggagaat tggtggatag gtctatccaa ggaaaaacaa ggttccttca
     9781 ccattcccta ctatgtcttg ggccaagact ggcagatttt catttctgac caacccctag
     9841 atggtttagt cgttacacct tttgaagctc cttatcaaga acactttgaa cgctggaagc
     9901 tgaatgctga gaaaaccctc ttttacggtt ctgtaaattt gcagcagtct gagagtctct
     9961 ttgcctatta cgaaccgatt tatagggtta ttattcaggg aaatctcaat caaatcgtag
    10021 aagagttaaa tctcttggag aaggtagttc ttgaaaatac gccgcgtgtt ccgattacta
    10081 aacagctttt tattcagttt gtcatggatg ttttccattt atttgaacat ctcaaagctg
    10141 atgacctgac agacatcgtc aaaaccattc atgctattca atccttcgat gaattggttc
    10201 cttatatcaa ggaaactctg agcagctttt tcggtcaata ccgtatgaat gaaaatgtgg
    10261 tcagtgtgct ggaagtcatt ggtcgtgatt accaaaaaga gctttccctc aaggatatca
    10321 gtaaggctct ctttatcaat cctgtctatc tagggcagtt gattaagcgt gaaaccgatt
    10381 cgacttttgc agagttacta aacaaacaac gtattaaggc tgcccaacaa cttttgcttt
    10441 caactagtga cagcatcgaa gatatttgtt atgctgttgg ttacagtaac cttggatatt
    10501 tctataaagt tttccgaaaa ttgtgcggaa aatcgccaaa agcctaccga aaacaggtag
    10561 aaactatact ataagatttg tattccttta caaaaggtgc tataatatta tcaggaggtc
    10621 ctatcatgaa aaagaaacca atttatctat gggtcttgct aatcttgtct gcccttattt
    10681 cagttccgtc tctgtttgga attgtgagtc ccttgcctag taaagaagcc cttcgtgctg
    10741 ctcagaaaca agttgcaggg gtcaatgctc agcaattaga agaccagctt aattatacct
    10801 atagagtagc agaagcatct cattctattt ttaatgttgc tttgattgtg ctatctacta
    10861 ttttagtggt cgtagcgatt gtcttccttg ttcgtaaaaa tttgcaatat gcaaactata
    10921 cttacgttgg ctatgttttg ttagctatta ttggttcgat ttatggctat gttggtttac
    10981 aagacgctgt gcagttggtt caagatgaga gcatgcgttt gacagtgagt attggttcaa
    11041 aagctgttag cattttctat atcgtcatca atgttctctt cctagccctt gtcttctata
    11101 agatgtggcg acaacagaaa gccttggcag aagaagagga aacagaagag cttacctaag
    11161 tattgacaaa aaagaactat caagatacaa tcttggtagt tgttttttga ttatagataa
    11221 aatcatggag gtacatatga agacaatttc tttagtttat attagtttga gtggcaacac
    11281 tgaaagtttt gtgacgcgct tgaaagacta tctcttgtcc cagtacaaag ggattgaggt
    11341 tcaaaagatt catatcaagg atttggtcaa ggaaggcaaa aatttctatg aaatggacca
    11401 tccctatgtc gcttttttgc cgacctatct cgaaggtggg aatggcgtgg ataacggaga
    11461 tgttgagatt ttgacgacac cggtgggaga ttttattgcc tatggtaaca atgctagtaa
    11521 gtgttttggt gtggttgggt caggaaatcg taactttaat aaccaatact gcctaacagc
    11581 caagcaatac agtcaacgct ttggcttccc tgtattggct gactttgaaa tgcgaggcat
    11641 gctggaagat atcaaacatg tcgcagctat tatcgcagat ttgtatgagt tggaaaagga
    11701 gaattaatac tcaatgaaaa tcaaagagca aactaggaag ctagtcgcag gttgctcaaa
    11761 gcacagcttt gaggttgcag atagagctga cgtggtttga agagattctc gaatagtata
    11821 aagttcttat aagatgtggt tgaagttgtt caactgtttt ttgagtataa acagtcttta
    11881 agaacgtaaa aaagcataag ttcaggtctt caaaaccttg ttcttatgct ttttatcata
    11941 gaaatatttg ctgaattttc tcttgaaatg aaaccttatc ttcatctcgc gcttgtccct
    12001 taccctgctt ttccgattgc gagtgcgtag ataaagaaga tggcgaagcc aaagaggaaa
    12061 atcaatcccg caatggcaag gtgtttgagc caagaaggaa gatttttgtt ttcacgtgtt
    12121 accaaggcgt aattcaaaag agcaaagaaa ggtgttgtca ggaaagagcc aatcatggca
    12181 aagcggagca tggttgaaac ctgaccagcg aagaacttga taatgacgat accgatgata
    12241 gcagtgatgg tcatccagat gttcaaagat ttacgattgt cctctttttg actgattagc
    12301 agtcggagag attcctgatt aacgcgagaa tagccatcga taacagttat aactgttcca
    12361 aagatacaga ggaaggcaat aaaggtaatc aagtaacggg accattcgcc aagaacagag
    12421 gcatacatgc ccacgaattg agagatgtat ttggctgaag cagcttcaac cgcctgccct
    12481 gtaggatact gaatcagtgc tcccagtgcc acaaagaaga cggctaggat agctgttcca
    12541 atataaccag tgttaaagtc aaacagagcg tcttctgtgt taaagttgac ggtctttctc
    12601 ttttcagctg accaaagtga attgatggct gaaatttcaa taggagccgg catccatcct
    12661 aggagggaga cgatgaaggg cagagctgcc atttgccaag gtgtcttctc gacaaaatca
    12721 gaactgtatt ctggatgctt gaccgccgca atgataactg caagaacagt cgcaatggtt
    12781 aaggcagaca taatccattt gaccatgccg tctaaaagtt tgtagcctcc aaagagtagc
    12841 atagcccaaa tgattgcaac gagaatgagg gaccactgag taatgctaag tccaatcatt
    12901 gggaaggcac tggcgatgat agctgagcac agaatggcaa caccagccgt gttgaccata
    12961 gccgaaaaga cattgagaat aaagaaaatc cagagataga gttttccttt ttcggcataa
    13021 ccttcaacca aagtctttcc agtatcagct gtgtattcag caccaaaacg gaaaaatgga
    13081 tatttaaaga cattggctaa gatgaccaag agaagtagag accaaccgta agaaccgcca
    13141 gcttgagttg aggatacaat gtgggaacct ccaacagcgg cagttgccat taggattcca
    13201 ggtcccattg cttttagctt gcttgcccag gttgattgat ttaaggaaat agcttgcgac
    13261 atgataaata ctcctttttg aattttcaga ataatctgta tatgcaatct atattctaca
    13321 aaaaattttg gaaaaatgca agaatatttt gaaaaaagat agaaaattta caaagaaaat
    13381 ctgaaataac tgttatg
//
LOCUS       DAVMEI010000042        13231 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVMEI010000042 DAVMEI010000000
VERSION     DAVMEI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.042"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(46..693)
                     /locus_tag="VYQ24_000947"
                     /pseudo
     CDS             complement(46..693)
                     /locus_tag="VYQ24_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(802..1320)
                     /locus_tag="VYQ24_000948"
     CDS             complement(802..1320)
                     /locus_tag="VYQ24_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1968105.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENIHSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1547..2416)
                     /gene="rsmI"
                     /locus_tag="VYQ24_000949"
     CDS             complement(1547..2416)
                     /gene="rsmI"
                     /locus_tag="VYQ24_000949"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1968106.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETSLKGECLLIVEGASQDVEEKGEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2419..2736)
                     /gene="yabA"
                     /locus_tag="VYQ24_000950"
     CDS             complement(2419..2736)
                     /gene="yabA"
                     /locus_tag="VYQ24_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1968107.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2776..3666)
                     /locus_tag="VYQ24_000951"
     CDS             complement(2776..3666)
                     /locus_tag="VYQ24_000951"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1968108.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3663..4301)
                     /locus_tag="VYQ24_000952"
     CDS             complement(3663..4301)
                     /locus_tag="VYQ24_000952"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1968109.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILKEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4521..5318)
                     /gene="proC"
                     /locus_tag="VYQ24_000953"
     CDS             complement(4521..5318)
                     /gene="proC"
                     /locus_tag="VYQ24_000953"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1968110.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5322..6584)
                     /locus_tag="VYQ24_000954"
     CDS             complement(5322..6584)
                     /locus_tag="VYQ24_000954"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1968111.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(6594..7724)
                     /locus_tag="VYQ24_000955"
     CDS             complement(6594..7724)
                     /locus_tag="VYQ24_000955"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1968112.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7804..9687)
                     /gene="cbpE"
                     /locus_tag="VYQ24_000956"
                     /gene_synonym="pce"
     CDS             complement(7804..9687)
                     /gene="cbpE"
                     /locus_tag="VYQ24_000956"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1968113.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9690..10577)
                     /locus_tag="VYQ24_000957"
     CDS             complement(9690..10577)
                     /locus_tag="VYQ24_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1968114.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10567..11028)
                     /locus_tag="VYQ24_000958"
     CDS             complement(10567..11028)
                     /locus_tag="VYQ24_000958"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1968115.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAILKEEINGN"
     gene            complement(11025..11933)
                     /locus_tag="VYQ24_000959"
     CDS             complement(11025..11933)
                     /locus_tag="VYQ24_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1968116.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11926..12123
                     /locus_tag="VYQ24_000960"
                     /pseudo
     CDS             11926..12123
                     /locus_tag="VYQ24_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(12175..12846)
                     /locus_tag="VYQ24_000961"
     CDS             complement(12175..12846)
                     /locus_tag="VYQ24_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1968117.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12900..13121)
                     /locus_tag="VYQ24_000962"
     CDS             complement(12900..13121)
                     /locus_tag="VYQ24_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1968118.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
BASE COUNT         3857 a         3079 c         2221 g         4074 t
ORIGIN      
        1 ttcggattac gaatgaattt gattgctggt attatcaatc atgaactagg attctagttt
       61 tgcaggaagt ctaatgtaat tctcaagctg tgagttcatt atgatttgta aatcctatat
      121 atcttcagca gctaaaaaca cattttgaaa tgttccaagt ccgttttcac caaaactata
      181 ttctctctta ctatacttac tcttattatt ctctatatag ttagagtcaa ggttcttaac
      241 ctcttgaagt tcattttctt tacttctgag gttaaaatct tttacttctg aagttaagtt
      301 ttttgacttc tgtaagtcat tttcttttac ctgaaatata ctcataaagt ctttaacata
      361 aatgatgttc ggcttaccaa gtccaagcct tactctttcg atcagtccta ttcctttttt
      421 tatatcaagc tcatctaatg tttttatggc agttggcttt gagatatttc ttcttttcat
      481 aatttcttcg acagtaaaat agataaatac tctgccttcc ttgtctatcc agttattctt
      541 aaatgacatt cctgtctatt taagaagtat ggaataaagg ataattgctt ctgctgataa
      601 gccccgaaat tcttctctat caactaatat ctccggtact tttaagaagt taaatctttt
      661 cgcttctctg ttataaaaat attcaaagtc catgcaagat cacctccctt cttaaaaatt
      721 tgcaaagaaa aagacgatag tttttcttct atcgcctttg gtaaccattt ttatgaaatt
      781 ttttaaagtt aaaaatataa tttataatac tatcttcttc cattttctga ttttagttct
      841 gaaagttcca aaaggtgcaa ccgtatttac atgaataaac ttatacactt cccaagtcgc
      901 tgttttagtc gcttcatcag cccactttct catatgcggt tcaaataatt cttcctcact
      961 caacgaatca atcattgcag agatagaatg aatattttca tttaattttg ctttcaactc
     1021 ttgcagagat aaatgagcgt aggtatctgt gaaccactga tataattcac caagttgatt
     1081 ccatttaaat ttatccgatg gtgtttttac ttgaagcccc tttctttcat cttcttccca
     1141 tttaagaacc aagttggtcc aaccaacctg ataagcaagg ttttctgctg gagttctgtc
     1201 aacttcatca gctctcttat cttttaaatt ttctggaata ttatcaaatt ctaaaatata
     1261 tttctcaaat gttttctcta tctcagcttt tagttcttct ttattttcgt atgttcgcaa
     1321 aaaatcacct tctttctgat ttatcaatcc tttgatttaa aatgacctta tttttctatt
     1381 tataccttct aagaacgcta taactgctgc tccaaccatc ggtattccgt atgggcttaa
     1441 taaaaaactt aataccaaag cttctatacc aagtgataca tctttatatt atcatcacta
     1501 attaagttaa acagaaacag acagaattat tacatcctgt ctccctttat tgtttttctt
     1561 cccagtcgtg gtaggcagcg tagagttgac tcttattcca ctggtaaatc ttagctactt
     1621 ccttaatagc ttgatttttc ttcatacctt gctgaatacg agcttggatt tctaagaaca
     1681 agtcttcctc gcctttttct tccacatcct gactggcacc ttcaacaatc agaagacatt
     1741 cacccttgag agacgtttca gcaatgcttt ccagcaattc agaaattgta cctctttggt
     1801 attcttcata gattttggtc aattccctga ccaagacaac ggagcggtca ccgtagactt
     1861 ctagcatatt ttccaatgta tctgccacac gatggggcga ttcataaaaa atctgagttt
     1921 caggatagtc ttttttcgag tcgaaaaatt gcttttgctg gcctgatttt ctcggtaaaa
     1981 agccgtaaaa gatatgtggc tgcggcgcta aaccactggc aatcaaggcg gaaattcctg
     2041 cagaggcacc tggaactgta acaactgcaa tttcttcctc aatagctgcc ttaaccaaat
     2101 catgaccagg gtctgaaatg ctaggcaaac cggcatcaga gacctgagca atactttgcc
     2161 ctgctttcaa gaaaccaatc aaatcaggaa ttttttcctt ggcattgtgc tcatgaaaac
     2221 tgatctgctt ggtggaaatg tcaaaatgct tgagcaaaag ccctgtattg cgcgtatcct
     2281 cagcagcaat ccagtccact tctttcaagg tctggatagc acgaaaagtc atatcatcta
     2341 gattgccaat cggcgttgcc actagataca gcttgccata gggagactgc cccttaaaac
     2401 ttttttgaat ttgcatgctt actccctgta taacaactca tcacagaaca tacattcttc
     2461 gtcctgctct cgacgttgtc cataaaaatc attacatacg tgaaatccat cacggtaaat
     2521 acgacggaca ctttcgcgaa catgcttggc cttgacggga gcgtctgctt ccacctcacc
     2581 caagcgttct cgcaacttac tattttccaa gcgaagagct gtattttctt ctaccaagct
     2641 cttgagattt ttcttgatgg cttccacatc ggctaaggtt accaataatt gttgggaaaa
     2701 atcatccagc gcgtcaaata attctttttt gtccataaaa cagccctttc ctttctttat
     2761 cattcatttt tgagtttata tttctttcaa gaccagatat tccatggcat tttgaaagct
     2821 gacattagct tgccacattt ttctagcttc tagtaaatct tgtagaatca ctcttactct
     2881 tacctgcaag aggtcctgcc cacagagaac ttcaagaatc cgtaaaacct gatcctgttt
     2941 ttccttatca tctgccaagt tggctaattt ggcaacctgt agataacttt cttttttctt
     3001 agctactaac caagtcagca ggcgttcact ttcatcgacc aaggtccaaa aacttgcctg
     3061 attagccaac ttttctgctt cagctcgcga ttgactaaac ttagctaaaa gagtcgcttt
     3121 tttcttaaca agtcccattt gttctaagag taagataagt ttttcttctt gctttttaaa
     3181 gtggaagatc tgagtccgac ttcggattgt cggtaacatc ttttcctcat cgctagtcaa
     3241 gaagaaaata taaacttcac tctggggttc ttcgatgacc ttgagcagag aattggctgc
     3301 gttgggatgc attttatccg cttgctcgat aataaagacc tgttgctggc tttcaatccc
     3361 tgcttgagaa aactgtccca ccaattcccg aatgcgttct gtcttgatga cctgattgac
     3421 tggcttaatc aaggtgacat ctggaaactc ttcctgttca atcagcttgc aacttcggca
     3481 tttctcacat ggtaagacgc caactttatc cgtacaaaag aggctcttag ctaaaaattg
     3541 cgccatttcc aagcttccaa agaaacctga aaagagatag gcgtgattga gctggtcttg
     3601 ttctaagata cggacaaaac ggtcaaactg agctggttgc caagccttta gttgatcttg
     3661 tttcatttgg ccaagcccat tccgtcaaac aagacagcct tggtagtttc cacaacttgc
     3721 tccaaaggga gactagcatc aatcttgaca atgcgatttc cctctttatc cagaagagaa
     3781 aggtagcctt gacgaacttt tttatgcaag tccaaccctt ccaaatccaa acgattaacc
     3841 tcgcggtcac tattcgcagc aatacgagcc agcccttctt ccacctcgat gtcaaaatag
     3901 agtgtcaaat cgggtttgag gccatctgtc gcaaactgat tgagccagtc aatggcttca
     3961 atatctaagc cacgaccaaa tccctgatag gcaacagaac tatcgataaa acgatccatg
     4021 atgaccaact tgccagcttc aagggctgga agaacttttt ccaccaaatg ctgtctgcga
     4081 ctggcaatat agagaagtag ctctgtttta gcatccatct gagtatgact tggatccaaa
     4141 atcacttccc gaatcttctc cccaatcaag actccgccag gttcacgggt cgtcaacacc
     4201 tctactcctt tttcctttaa aattggtagc agagcctcta aaacactggt cttgcctgct
     4261 ccctctggtc cctcaagaga gactaaaaat ccttttgaca tgtctaactc atttcttttt
     4321 tactacttct attctatcaa aaaaataggg gtttgtgaca atctttttgt cttctcattt
     4381 catactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggctgt acttgagtac
     4441 ggcaaggtga agctgacgca gtttgaattt gattttcgaa gagtatcaac caccataaaa
     4501 gaggcagata aaactacctc ttatttacct agttcttgtg ttcgtttata ggcttgatga
     4561 actgcctcca tgactgtgcc tcgaaaagca tgcgcttcta ggcttgctac gccagcaata
     4621 gtcgaaccgc ctggactaca aacttgatct ttcaaaactc caggatgttg ctggctttct
     4681 aggaccaatt gcccagctcc taccacagtt tgggctgcca ttttcaaggc tgtttctcgt
     4741 ggtaatcccg tctgaacacc tgcatctgcc aaggcctcaa taaagagata gacaaaggct
     4801 ggtccacaac ctgcaagacc tgtcgctgca ttgattaagc tttctcctaa ttcaaccaag
     4861 agaccagcct tggctaaaag ctgacaaaag atctcactgt cctcagccct gcaattagga
     4921 gacaaggcat aactaatcac tccttgcccg atagaagcag gggtattagg catcatacga
     4981 ataattcggt gttggcttgg gataagactt gctagttttt ctaaggtcaa tccagctgcc
     5041 atcgaaatca aaagaagact ttctcttttt tcaaggatgg tctgatattg agaaagcagt
     5101 tcagaaaact gagcaggctt aactcctaga aaaatcacat ctgcttctgc aaacatttct
     5161 tcattgctgg aagcctgacc accaaagtct gcaatgaaag catctacctt agcttgacta
     5221 cgattggcaa ggagaatctc atctgacgtc ctagtctgca agacagattt tgccaagcta
     5281 gcacccatat tccccaaacc gataaatcca atcttcatct cttactccct tatctgccca
     5341 tcaccagcaa ccacatactt gtagctggtc aactctttca agcccattgg accacgcgcg
     5401 tgcaatttct gagtagaaat ccccatttca caaccaagac caaattgtcc tccatctgtg
     5461 aaacgagttg aggcattaac atacaccgct gcagagtcca cttgatctgt aaagtatgct
     5521 gcagcttcag cattttccgt cacaatagca tccgaatgat gggtactgtg ggattcaata
     5581 tgcgcaaccg cttcttctaa actgctcaca accttaacag ctagaatata gtctaaaaac
     5641 tcggtatcaa agtcttgtgc ttgagcagct tgacctgaaa caaactggct tgctttgcta
     5701 tctaggcgga attgaattgg ttccaaccca gcttcttttc gatctgcaac cagcatttga
     5761 tccaagcgag gaaggatgct tgctgccttg ttttcatgaa ccagcagaac ctccatggca
     5821 ttacaaacag aaggacgact ggttttagca ttgttgatga tagacagcgc cttgtcttcg
     5881 tctgcatcct tatccacata gacatggaca atcccagtcc ctgtctcgat aacaggtaca
     5941 atcgcattct caaccactgc attgatcaag ccagctcctc cacgaggaat gagaaggtct
     6001 agatagccct tggccttcat catagcataa ctactttcac ggctagtatc ctccaccagt
     6061 tgaatcacat ttggatgaat agtagtcgtc tccaagccct tcttcaaggc tgtgacaatg
     6121 gcatgggttg tttgataggc atccttacca ctacgaagaa caaccgcatt tccactctta
     6181 agagtcaaag cagccgcatc agacgtcaca tttggacggc tttcatagat aataccgatg
     6241 acacccatag ctacacgttt ttttgtgata accaaaccat tttcaagctg acttgtttct
     6301 aaaacttcac cgattggatc tggtaaggca accacttcac gaattcctct tgccatcgct
     6361 tctatacgat ctgcatccaa ataaagacga tccaacatca catctgagat tttcccctta
     6421 gccgctgcca tatcgagggc attagccgct aaaatttcct cagtagcagc cactaagtga
     6481 tcagccatgg ctagcaaggc ttggtttttc acttcttcac tagctgtgtt aatcgatttt
     6541 ttaacagcct gtacctgttc aaattgttct tgtctactca ccatagttta cctctaaaat
     6601 tctgtaaaaa gtagttggat ttcaggagta atggaaatcc agtcgtcacg gtaaatcaag
     6661 acacccttgg ctttttgaga acgcaacata tcctccaaag cagatgctcc aaattgcacg
     6721 cgtccttttc caagtgattt tccactttcc ttatcaaata ctgtcacgat atcaccgtaa
     6781 gaaaagactc cttctgcttc aacgatacca gataagagaa gactctttcc atgttgagag
     6841 agagcttccg cagccccttt atcaacccaa atagaacctt gactctgagc atagaaggca
     6901 agccattgtt tctgggtacg aagccccttc tcttgagcaa caaagtaaga accatcctcg
     6961 gtctcctctg ccgcctcaat catggaatct gatttcaagg atgagcagat ataaacagga
     7021 actcctgatt ccgtcgcgat agttgcagcc ttgattttgg ttaacatacc cccagttccg
     7081 tttgacgaac cagctccacc agccatatca ataatctcac gattgatggt ctcgattctc
     7141 tccaagcgtt tggctcttgg atctgaatta ggatttccag tatagagacc gtccacatct
     7201 gtcaagaaaa ctaaaaggtc tgcttggacc atcgccgcta cttgagcact tagagtgtca
     7261 ttgtccccaa ccttgagctc atcaataacg acactatcat tctcattgat gataggaatt
     7321 gccccacggt tgagcaaaac cgacaaagcc tgatgggcat ttttataacg acgcttatcc
     7381 acaaagtcat cttgggtcag caagatttgt gcagaaacga tttgacgcaa gagaagattg
     7441 gtcgtatatt cttccaacaa aagtccctgc cctaccgctg ctgaagcctg tttatcagca
     7501 attttagtcg gacgcttttt aaatcctaag gctccaaaac cagccgcaat ggcacctgaa
     7561 gacaccaaaa tcaactcatg accagcctcg tgcagcatag ccaactgctg ggtaatatcc
     7621 tttaccttac tacgtgataa acttccatcc tcatttgtca gagaagaagt acccacctta
     7681 aagacaatcc gtttgtattt catagtctca ctccgattat tcatatttat ccattataac
     7741 atgaataaca ggaaatagaa aagagttgat aggaaaaaga aggccctaaa accttctttt
     7801 ctactactgt tctgattccg atttgtttac actcgtttca gagctttgtg aagttgagga
     7861 tgtcaaatct tgactagttg aagaatttgt aacactttca tgttgactcg tttctttatt
     7921 ttcaagatcc tttttcacta cttctttgga ttcttttatt tctgaatgac ttgtagtttt
     7981 aattgtagca gatttctcga tcggaacatc cactaaccag gcaccacttg aatccacagt
     8041 atagccttct ggtgtcttac cattcacaag taattgacca ttttctttca agaagtacca
     8101 tttatccttg tctttaatcc aacctatagc tcgtgaacca tctgtcttgt agaaatacca
     8161 atcatttgcc tttttaagcc agccttgctt catagctcct gattcttcta gatagtaatg
     8221 atgtccagca acttctatct cacctgtctt catgatacca gtagaatcca tatagtacca
     8281 ggcttcttta tcttttaccc agcccgtttt catttcccct gatggagcaa agtaatacca
     8341 ttgcccctta tattgaagcc aacctgtctt catagagcca tcaacatcca aataatacca
     8401 ctgctcttta tcttgaatcc aaccaatttc catctggttt tctttgttaa aataatacca
     8461 gattccagcg attttcttcc aatttttagc agcaacacca gagtctgtca aatagaacca
     8521 acgattgttc cattttttcc attgattctg taataagata cccgtttggt taaagtaata
     8581 ccattcaccc ttaatttcat tccaaccgac agcatattct cctgtagaat caggcgcttg
     8641 ataccaccag tttccatatg aactcttatg ccaaccagct tgaaaacttg gaatcggctt
     8701 gtaggaagtt gaaatgttta ctaaaccgtc ttgtcgaata tcaaaaactg ttgcatcata
     8761 gtctttgctg gctgcattga ttctctcaat tcctcgttct ttgagccaat taacatactc
     8821 actatcaaca ccatttttcc aaggtagact atccgaagtt tgaacaatca aactcggact
     8881 caaattttta atgaaatcct tggtatttga tttgttggta tcatggtgat gattaaactt
     8941 catcaaatca acttttccaa tgagaggacc atacttgtct tctgctccat gaacattatc
     9001 taaatcgccc ccaaggtaaa ttttcttgcc attgactttc accacgctaa tcaaggaatt
     9061 ggaattgtca tcccaaattt tctttaattc acccgatgaa tcagtttcat tttcataatt
     9121 atagagctga atatccatgt ccccaaactg aaaatgagca tccccttgtg tgatattttg
     9181 aataactgaa acaccttttt ctgcagcagt ctgtaaaacc ttatcatagc catacagatt
     9241 atcccataga cgttcagaat tagtaatacg actatcacta tatttcttaa gatagactcg
     9301 gtcaactgga taggtagaca gtaattcatc aacatttcca atatgatcac tgtgggtatg
     9361 ggtcaccaaa ataaaatcaa gtttttggac acccaattcc ttcaaacgac gaaagacacg
     9421 gtctgttaga acatgcttat aagacgtttc aattccttct ctccatggat agcgagaatc
     9481 acttccatct gggaaatcat aatcttctcc tgtatccacc atggcaaaat gtccattgct
     9541 ttcaagaata atcgcatcac tgccaccttc ttgaacattg ataaagtgga ttttatttcc
     9601 tgaactttct tgagcctgaa cattcccata ccatgacaaa cctaaaaaag cgcctacaag
     9661 tgctaaacta gttaatttct ttttcattct tactttctca atctctccaa ggtttcctta
     9721 aaaatctctg ggatatctgc tttaaattcc aaggtcttac ctgttctcgg atgagtaaaa
     9781 cctaaagtct tggcatgaag aaattgtcca tgtcctttca aagtcttgcg aggaccatag
     9841 acctcatcac cagcgactga atggccgata taagccatgt ggacacggat ttgatgagtg
     9901 cgccctgtct ccagttgcaa ctctactaag ctataatcgc caaagcgttc caagacgtga
     9961 aaacgcgtca ctgcaggctt ccctttagca gttacagcct gtttcttacg gtctttttca
    10021 ctccggccaa tcggcgcttc aattacacca cgatcattag gcagatttcc atgaacaatc
    10081 gcccaatatt tgcggagaga ctttttatct ttgagttctt gggcaagtac tagatgcgca
    10141 tcatcgtttt tagcaatcat gagaagacct gacgtatcct tatcaatacg gtgaacaatc
    10201 cctggacgca gaaccccatt gatacccgac aagtccttaa tatgatacat gagggcattt
    10261 actagggttc cactggtatg accagcactc gggtgcacaa ccattccctg aggtttgtta
    10321 acgacagcca catcctcatc ttggtagact atttctagcg gaagatcctc agccacatac
    10381 tctaatacct ctggttctgg cacatggtaa gtgacgacat caccctcttg gactgtgtat
    10441 ttagctttct tgacttgacc attgaccaag acctggcctg atttaatttg ttcattcgcg
    10501 agactacgtg ataattctga caaatctgac aaagccttat ccaaacgcag accaccagtt
    10561 tcaattttaa tttccattta tttcctcttt tagtattgca atcaataaaa taatcactcc
    10621 aaccgtcaga tagctatctg ccacattgaa aattgcaaag ttgataaagt caaggtggaa
    10681 catatccaca acaaagccct gactgaccct gtcaataaag tttccaagac cacccgcgat
    10741 tattagagtc aaacccaaga ccatccagaa tgagtcctcc atgtgtttat gtaaatacca
    10801 aatggcacct atcacgacaa ccagagtaat gacagcgaat aacagctgct gatcttgtaa
    10861 gatagaaaag gctgcacctc gattttgcag gtaggtcaag ctaacgaaat tgggaatcca
    10921 ggagcgcact tcacccagtg gaatctgctg gacgatatag gatttgacca actgatccag
    10981 cccaattaaa agcagtacaa tgactgccac tattgctctt tttttcatga tttcctcttt
    11041 tgatcaaaat attcttgcat gacttctacg aagagagtcc cagcttgact aagctccact
    11101 tcttcacgtt taacatagac catgcggtta tctaggttat ccttgagacg aataactgta
    11161 atgccattaa cactgtcact atctaaaaat ccagaacctg tcgcataggc gtccgtccgc
    11221 tccaaaatac cattcaaggt ggcacggtct gtcacattaa acatctgtga gctagcgctg
    11281 gtatcgacaa agttctctga ataataaagg tactcgtctt tctcttgagt gaaacgaacc
    11341 gttggtaaat ccgctaaatc ctccatgact aattcctctt tctgggctaa aggatgaccc
    11401 tcacggagat aaatatgggt atggaaagga atcaattcga tgacctccag acctaatttt
    11461 tcaacccgtt gcataatccc ctttttattt tgattgttga ggtagataat cccaatctca
    11521 ctatgccctt gcgccacttc atctaatatt tgaacagtag ttgattcaaa aatacggaag
    11581 ttcttatagt caggatagcg ctctgaaaag gccgtaatag ttggtggcaa gaagtcatag
    11641 tgctggctag caacagaaaa ttcatctttt tcttcttcag gattggcata ctgattttga
    11701 aaaatatcaa atcctttaac caattcttgc gctttttcat aaaattccat cccacgacgg
    11761 gtcaagaaag tccctgagct ggtccgacgg aaaatcttaa aacccaactc tttttccaaa
    11821 tcacgaacag aaatagacag actcggctga ctaacataca tcttttcagc agcttcacgg
    11881 aaagtaccac tattggcaat ggccacaaca tagcgtaatt gttgaatgtt catcttctac
    11941 ccctaacttc tttatctttt cattatacca tattttagaa gttttccaaa aaggaaaaaa
    12001 gaacatcctc ttcttcttaa ctatcttcac tatctgcctt tctcaagcca accttatttt
    12061 caaaatcaat tcaaaaaaat aactggatac acaccactac aatatcgaaa agaaaaccgt
    12121 tgactatttg tgaaaaaaga aaatgccgga aagttccgac attattttag taagctaact
    12181 tcctgaaaat aggagcaacc acacatccca gaaggcagtg atattaatga gaatatgaac
    12241 tagtattgga tagtagagat ttttagtcat gcgatacaat agagctaaaa taagacctcc
    12301 actagcataa atgaaaaaag caagaggagt taaagcaaaa ttgatatgaa tataaccgaa
    12361 aataatagca gaaagcaaaa catctccgta ccaaggtgag tttttgaaaa aggttgtcat
    12421 aagcacacct cgataaatca attcctcagc aataggggcg atgaagcaaa cgatgagcaa
    12481 gaaataaggg aactcctgtc tccccatcat ttctaccgtt tcattcaaag aaatctgatt
    12541 tgaagacagg gatatgaaat acgaaaagag gaagtcagac atatatgaaa tgatgtagcc
    12601 cagtaaaagg tagatgaagt acctcagctg ccacttgaaa tgaaaaattt tctttcctgc
    12661 aaaaaccagt agatagatga ccgctaagaa aagaactccg ctctccatca aaagcagaat
    12721 ctgaaaaaat tcacgacttg ctggtagata gggctttgcg aggtgattga aaactctcca
    12781 aaaccaagtt gatttgtaaa aaattaagga caatgctagt aaaatttgaa tagcccgttt
    12841 tttcatatta aattctctgc tttctccact tgtttcttca tattactaat aaattttact
    12901 taaaatctcg cagcacttcc tttgcaaaga tgattgcctc ctccaatata tcctaaatca
    12961 taggtgcctc tgggacaaaa tccatgaacc ggcagagtta atactggttc tatttcccaa
    13021 tcgtacctac cattaggatt tttaccatat gaatctgctc caccttgtat cagctctaat
    13081 tcttcatttg ttagatttat attttttgtc attaaaatca tgatatattt cctccattat
    13141 tcttcagatt agttgagtaa tctgatactc atacctactt acaaaaaaac tattatatta
    13201 agttggtttt ttaatttgtc tagttgcaac a
//
LOCUS       DAVMEI010000043        13216 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVMEI010000043 DAVMEI010000000
VERSION     DAVMEI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13216
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.043"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..485)
                     /locus_tag="VYQ24_000963"
     CDS             complement(<1..485)
                     /locus_tag="VYQ24_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1968119.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     Q"
     gene            607..1353
                     /locus_tag="VYQ24_000964"
     CDS             607..1353
                     /locus_tag="VYQ24_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1968120.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNMATLENAGIMVKVVS"
     gene            1366..2346
                     /locus_tag="VYQ24_000965"
     CDS             1366..2346
                     /locus_tag="VYQ24_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1968121.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            2541..2861
                     /locus_tag="VYQ24_000966"
     CDS             2541..2861
                     /locus_tag="VYQ24_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1968122.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            2907..3215
                     /locus_tag="VYQ24_000967"
     CDS             2907..3215
                     /locus_tag="VYQ24_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1968123.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3232..4530
                     /locus_tag="VYQ24_000968"
     CDS             3232..4530
                     /locus_tag="VYQ24_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1968124.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPTIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            4681..7119
                     /locus_tag="VYQ24_000969"
     CDS             4681..7119
                     /locus_tag="VYQ24_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1968125.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7138..7806
                     /locus_tag="VYQ24_000970"
     CDS             7138..7806
                     /locus_tag="VYQ24_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1968126.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            7824..8912
                     /locus_tag="VYQ24_000971"
     CDS             7824..8912
                     /locus_tag="VYQ24_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1968127.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            9367..11868
                     /locus_tag="VYQ24_000972"
     CDS             9367..11868
                     /locus_tag="VYQ24_000972"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1968128.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLVRTDLAKEKTGVWTGAYVINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHIETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            12062..12757
                     /locus_tag="VYQ24_000973"
     CDS             12062..12757
                     /locus_tag="VYQ24_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968129.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            12769..13185
                     /locus_tag="VYQ24_000974"
     CDS             12769..13185
                     /locus_tag="VYQ24_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968130.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
BASE COUNT         4229 a         2065 c         2807 g         4115 t
ORIGIN      
        1 atttgattaa aaaccccagt cactttttta tgttttatag aattataatg ttttaggtct
       61 gtgtagataa aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca
      121 gtagtttcaa tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca
      181 aattcaaact gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg
      241 taaaattctt tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg
      301 actaaggtga atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat
      361 tcaggattag aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga
      421 atacccggac cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt
      481 tccataagag ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat
      541 tacgaacgaa aggttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg
      601 tgtaaaatga aaagattgga acaaattatt aaattagtat cagaatatga aaagatcgac
      661 gttaatacat tatcggaaaa attaaatgta tcgaaagtaa cgattagaaa agatttagat
      721 aaattagagt caaaaggttt attacacaga gagcatggat atgctgtatt aaatagtgga
      781 gatgacttaa atgtacgttt gtcaattaac tatgaaatta agagaaaaat tgttcaggaa
      841 gcagtaaaat tggtgtcaga taatgaaaca ataatgatag aatctggatc gacctgtgct
      901 ttacttgctg aggaaatttg caagcaaaaa agaaatgtta cgattgtaac aaattcattt
      961 tttatagcaa attttgtgag agcttatgat tcatgtcgta ttattgttct tggtggagag
     1021 tttcagaagg attcacaggt tactgtggga cctttattaa aagaaatgat acagactttt
     1081 catgtgcgtc aagcttttgt tgggacagat ggctacgata aagagatggg ctttacagga
     1141 aaagatttaa tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga taaagtcatt
     1201 gtgctaactg actcaagtaa atttgataaa aggggtacag taagaagatt tgctttaagt
     1261 caagtctatg aagtaataac agacgaaaaa ctttctaaac aaaatatggc tacattagaa
     1321 aatgctggga taatggttaa ggtagtttcg taagaggtta agtgtatgaa tcaagatagg
     1381 aataaactgc tctctaaaat tgcttatctg tattatattg aaaacttaaa tcagtcacaa
     1441 atagcagcaa aattaggaat ttatagaacc tctattagta gaatgttaac agaagcaagg
     1501 aatgcaggaa ttgttaaaat tgaaatagag aattttgata ccaatatgtt taagttggaa
     1561 aattatgtaa aagaaaaata tggtctggaa agtttagaaa ttattccaaa tgaatttgat
     1621 gataatccaa caattttatc tgaaagaatt tctcaagttg cagcaggtgt ccttaggaat
     1681 ctaattgatg ataatatgaa aattggcttt tcttggggga aaagtttaag taatttagta
     1741 gatttaattc acagtaaaag tgtccgaaat gttcacttct atcctctagc aggtggtcct
     1801 agtcacatac acgctaaata ccatgtgaat acactgattt atgaaatgtc tagaaaattt
     1861 catggagagt gtacatttat gaatgcaacg attgtgcaag aaaataaatt gttagcagat
     1921 ggtattttgc aatcaagata ttttgaaaat ttgaaaaata gttggaaaga tttagatata
     1981 gctgtagtcg gaattggtga ttttagcaat aaaggaaagc atcaatggtt agacatgctt
     2041 acagaggatg actttaaaga attgaccaaa gttaaaactg taggagaaat ttgttgtcga
     2101 ttttttgatt caaaaggtaa agaagtctat gaaaacttac aggaaagaac gatagcaatc
     2161 tctttggaag atttaaaaaa tattcctcaa agtttagctg ttgcttacgg tgatacgaaa
     2221 gtatcttcga ttctttctgt cttgcgtgct aatttagtaa atcatttgat tacagacaaa
     2281 aatacaattt taaaagtttt ggaagaagat ggggatttga cttttagaga gattctaggt
     2341 gagtgaaaat gatagactga ttcagtttat cgtttttctt tttagttgat tgcacatttg
     2401 tgcttatata aacaaaaata gtttatctgt tgtttttgga ttgacaactt tattatgtag
     2461 ttgtattcta tagttacaaa agaaaatttt aaaatttcaa atgaaaaaag ctttttacat
     2521 agtgaaatga ggaggaattt atggaaatta ttgttccaga tcaaattatc atgggtttaa
     2581 ttttatatgc tggcgatgcg aaacaacata tttataaagc attagattac ataaagaacg
     2641 gtacatgtga acgatgtgaa gaagaaatcc agttagctga tgcagcctta ttagaagctc
     2701 ataatctaca aacaaaattc ttggcacagg aagcgtctgg tacaaagaca gaaattacag
     2761 ctctctttgt tcattcacaa gatcatctca tgactagtat gacggagatt aatttaatca
     2821 aagaaattat tagtttgaga aaagaacttc ataaaaaata atactagagt attatcattg
     2881 ttattaacat agaggaggaa aacataatgg tgaagattgg tttgttttgt gcagcaggtt
     2941 tttctactgg tatgcttgta aataatatga aaattgcagc gcaatctagt ggagttgagg
     3001 cagaaataga ggcgttttct cagtctaaat tagcggatta tgcgccaaat atagatgttg
     3061 cactattggg tccacaagtt gcttatacat tagataaatc aaaagaaatt tgtgataagt
     3121 gtgatgttcc gatagctgtt attccgatga tggactatgg tatgttagat gggaaaaaag
     3181 tattagattt ggccctatct ttgattagtg ggtaagaaaa ggagatttat tatgtcaaag
     3241 atggatgttc agaaaatcat tgcaccgatg atgaagtttg tgaatatgcg tggcattata
     3301 gctctaaaag atgggatgtt agcaatttta ccattgacag tagttggtag tttgttcttg
     3361 attatgggac aattgccgtt cgaaggatta aataagagca ttgctagtgt ttttggagct
     3421 aattggacag agccgtttat gcaagtatat tcaggaactt ttgctattat gggtctaatt
     3481 tcttgttttt caattgccta ttcttatgct aagaatagcg gagtagaggc tttaccagct
     3541 ggagttctat ctgtatctgc attctttatt ttgctaagat cgtcttatat ccctaaacaa
     3601 ggtgaggcga ttggggacgc tattagtaaa gtttggtttg gaggccaagg aattatcggt
     3661 gctatcatta taggtttggt agtaggaagt atttatacct tctttataaa gagaaaaatt
     3721 gttattaaga tgccagaaca agttccacaa gctattgcca aacagtttga agcaatgatt
     3781 ccagcatttg taattttctt atcttctatg attgtatata ttttagcgaa gtcattgact
     3841 aatggcggaa cattcataga aatgatttat tctgctattc aagttccgtt gcaaggttta
     3901 actggatctt tgtatggtgc tattggaatt gcattcttta tatcattttt gtggtggttt
     3961 ggtgttcatg ggcaatcggt agtaaatgga gtagtgacag ctctgctttt atctaatctt
     4021 gatgctaata aagctatgtt agcctctggt aatctatcat tagaaaatgg tgcacatatt
     4081 gttactcaac aatttttaga ttcattttta attctatcag gttcagggat tacgtttggt
     4141 cttgtagttg ccatgctttt tgcagcaaaa tcaaaacaat accaagcctt aggaaaagtt
     4201 gcagcttttc caacaatatt taacgtaaat gagccagttg tatttggatt tccgattgtc
     4261 atgaatccag ttatgtttgt acctttcatt cttgttcctg tacttgcagc tgtgatagta
     4321 tatggagcta ttgcaacagg tttcatgcaa ccattctcag gggtaacatt gccttggagt
     4381 acaccagcta ttctatcagg atttttggtg ggtggatggc aaggtgttgt gactcagtta
     4441 ttagtattgg tgatatctac agtggtttac tttccattct ttaagataca agatcgttta
     4501 gcttaccaaa atgaaatcaa acaatcttag aggtatttgt gtgttactgt taaactcaca
     4561 catttgtgct aaaaattaga gagttaaaat ttttctagtt aaaagcttga aaatttctat
     4621 aaaaatcggt attatatttt cgaaagaaat aaaaatattt tcgaaagaaa ggtgcttacg
     4681 atggtaaata cagaagtagc aagaacaaca atcaagacag aatattttgg cagccttact
     4741 gaaaggatga acaaatatcg agaagatgtt ttaaataaaa aaccttatat tgatgctgag
     4801 agagcagttc tagcaacacg cgcctatgaa cgatacaagg aacaacctaa tgtcctaaaa
     4861 cgtgcatata tgctgaaaga aattttggaa aatatgtcta tctatattga agaagaatct
     4921 atgattgcgg gaaatcaagc ttcttccaat aaagatgctc ctatttttcc ggaatatacg
     4981 ctagaatttg ttctcaatga gttggatctt tttgaaaagc gtgatggaga tgttttctat
     5041 attacagaag aaacaaaaga acaacttaga agtattgctc cgttttggga aaataataat
     5101 ttacgtgcta gagctggtgc cttattacct gaagaagtgt ctgtttatat ggaaacagga
     5161 ttcttcggta tggaaggtaa gatgaattct ggagatgctc acttagcagt taactatcag
     5221 aaacttttgc aatttggttt aagaggtttt gaagagcggg ctcgtaaagc gaaggtagct
     5281 ctagatttaa cagatccagc aagtattgat aaatatcatt tttacgactc tatatttatc
     5341 gtaatcgatg ctattaaagt atatgcaaag cgctttgttg ctcttgctaa aagtttagcc
     5401 gaaaatgcaa atcctaaacg taagaaagaa ttacttgaga ttgcagatat ttgctctaga
     5461 gtcccatatg aaccggcaac tacttttgca gaagctattc aatcagtttg gtttattcaa
     5521 tgtattttac aaattgaatc taatggccac tctctttcat atggccgttt tgatcaatat
     5581 atgtatccat atatgaaggc tgatttagaa agtggtaaag aaacagaaga tagcattgtt
     5641 gaacgtctga caaatctttg gattaagaca attacaatta ataaggttcg cagtcaatca
     5701 catacatttt cttcagcagg aagtccttta tatcaaaatg ttacaattgg tggacagact
     5761 cgagataaga aggatgctgt taacccatta tcttatttgg tattaaaatc agttgcacaa
     5821 acccatctac cgcaacctaa tctaactgta cgttaccatg caggtttaga tgctcgtttc
     5881 atgaatgagt gtattgaagt gatgaaactt ggttttggta tgcctgcatt taataatgat
     5941 gagattatta ttccttcttt tattgcaaaa ggagtattgg aagatgatgc ttatgattac
     6001 agtgccattg gatgtgttga aacggcagtt ccagggaaat ggggctatcg ttgcacaggt
     6061 atgagttata tgaacttccc taaggttcta cttatcacga tgaatgatgg aattgatccg
     6121 gcttcgggta aacggtttgc accaagcttt ggtcatttta aggatatgaa gaacttttct
     6181 gaattagaaa atgcttggga taaaacacta agatatttga cacgaatgag tgttattgtt
     6241 gaaaattcta ttgatttatc attggaacga gaagttcctg atattctatg ttcagcattg
     6301 actgatgatt gtattggtcg tggaaaacac cttaaagaag gtggagcagt atatgattat
     6361 atatcaggat tgcaagttgg aattgcaaat ttgtcggatt cattagctgc aattaaaaaa
     6421 ttggtgtttg aggaagaacg tataagccca agtcagcttt ggcatgcact ggaaacagat
     6481 tatgccggag aagaaggtaa ggtcattcaa gaaatgttga ttcatgatgc acctaagtat
     6541 ggtaatgatg atgattatgc tgacaaattg gttactgctg cttatgacat ttatgttgat
     6601 gaaattgcta aatatcctaa tacacgttat ggaagagggc ctattggagg aattcgttat
     6661 tcaggaacat cttctatatc agccaacgta gggcagggac gtggaacatt agcaactcca
     6721 gatggacgca acgcgggtac accgttagca gagggttgtt caccatcaca taatatggat
     6781 caacacggcc ctacatctgt tttaaaatct gtttcaaaat taccaacaga tgaaatcgta
     6841 ggtggggttc tcttaaatca gaaagtaaat cctcaaacgt tagccaaaga agaagataaa
     6901 ttaaaactaa ttgctttgtt acgaacattc tttaatcgtt tacatgggta ccatattcaa
     6961 tacaatgttg tttccagaga gacgttgatt gacgctcaga aacatcctga aaaacacaga
     7021 gacttaattg ttcgtgttgc aggatactct gcattcttca atgttctttc taaggcaacc
     7081 caagatgaca ttataggacg tactgagcat actttgtaaa ataaagaggt tctttttatg
     7141 gaatttatgc ttgacacatt aaatttagat gagattaaaa agtggtctga aattttgccg
     7201 ctagctgggg taacttcaaa tcccactatt gcaaaaagag agggttctat taattttttt
     7261 gaacgaatca aagatgtaag agaattgatt ggctctacac cctctattca tgttcaggtg
     7321 atttctcaag attttgaagg catcttaaag gatgctcatg aaattcgaag acaagcagga
     7381 gatgatatat ttatcaaagt acctgttact ccagctggat tacgtgcaat aaaggtgcta
     7441 aaaaaagagg gctaccatat cactgcaaca gctatttata cagttattca gggattatta
     7501 gctatcgaag caggagcgga ttacctagct ccatattata atagaatgga aaatctgaac
     7561 attgattcaa attctgtcat tcgtcaatta gctcttgcta ttgatagaca gaactctcct
     7621 agtaagattt tagctgcatc ctttaaaaat gtagcacaag taaataatgc tttagctgca
     7681 ggtgcgcatg ctgttacagc aggagcagat gtttttgaat cagctttcgc catgccatct
     7741 atccaaaagg cggttgatga cttttctgac gactggtttg ttattcaaaa tagtcgttcc
     7801 atttagatag agaggaaata catatgagaa tttttgctag tccttctaga tatattcagg
     7861 gggaaaatgc cttgtttgaa aatgccaaat caattttgga tttgggaaat tgccctattc
     7921 tattatgcga tcagttggtt tatgatattg ttggaaaacg atttgaagat tatctacata
     7981 ggtatggttt ccatattgtt ctggcgctat ttaatggtga agcttctgac aatgaaatca
     8041 atcgagttgt tgccttggct gagaaagaaa attgtgatag tattatcggt cttggtgggg
     8101 gaaagacgat tgatagcgca aaagctattg cagatttgat tgaaaagcct gttattattg
     8161 ctccaacaat tgcatcgacc gacgcacctg tatctgcttt atctgttatt tatacagatg
     8221 aaggtgcatt tgatcattat ctattttatt ctaaaaatcc agatttagtt ttggttgata
     8281 caaaagttat ttcacaagcc cctaagcgtt tattagcgtc tggtattgca gatggtttag
     8341 caacttgggt tgaggcgcgt gcggttatgc aggcaaatgg aaaaactatg ttgggacaac
     8401 agcaaacatt ggctggagtt gcaattgcga agaaatgtga agaaacgctg tttgcagatg
     8461 gtttacaggc tatggcagct tgtgaagcta aagtggtgac accagcatta gaaaatattg
     8521 ttgaagctaa tactttattg agtggtctag gttttgaaag tggaggatta gctgcggcgc
     8581 atgcaattca taatggtttt actgcattga caggtgacat tcatcattta acacatggtg
     8641 aaaaagtagc ttatggaact ttagtacaac tattattgga aaatagacct aaagaagaac
     8701 ttgataagta tattgagttt tacaaaaaaa ttggtatgcc aacaactcta aaagaaatgc
     8761 atttggatca agttggatat gatgatttaa taaaagttgg taaacaagca actatggagg
     8821 gtgagacaat tcatcagatg ccgtttaaga tttcgccttc agatgttgct caagctatta
     8881 tcgctgtaga tgcctatgta aattcaaaat aaacaataag gactactgtt ttccaaatgg
     8941 tagtctttta ttgatcctcg tattgaattc tatagcagat tgaaataaga tgagaacaaa
     9001 tcgattggga aagtaaaatt aatttctaac aatgttttag caattgtttc gtactatttc
     9061 agattcagtg cactatacgt tcttcataaa tcaaaaagcg acataggttg tcggctattt
     9121 attgtgaata cattaattag cattccagtt ttatcttcgg tctaaaataa gtattttgtg
     9181 ctatacgaga taagcttctt gacttactcc ttgatttact gcataacaat gggataaaaa
     9241 gtgggagata gagcaattca tagtcatcaa aattaatgag atacagtata cagtttttcc
     9301 tttaaacaca tttcaaattc cctcaaaaat ggtataatag taacatcaca aaattggaga
     9361 gagaccatga gtttttataa tcataaagaa attgagccta agtggcaggg ctactgggca
     9421 gaacatcata catttaagac aggaacagat gcatcaaaac ctaagtttta tgcgcttgat
     9481 atgttccctt atccgtctgg agctggtctg cacgtaggac acccagaagg ttatactgca
     9541 accgatatcc tcagtcgtta caaacgtgcg caaggctaca atgttcttca cccaatgggt
     9601 tgggatgctt ttggtttgcc tgcagagcaa tacgctatgg atactggtaa tgacccagca
     9661 gaatttacag cggaaaacat tgccaacttc aaacgtcaaa tcaatgcgct tggattttct
     9721 tatgactggg atcgtgaagt caacacaaca gatccaaact actacaagtg gactcaatgg
     9781 attttcacca agctttacga aaaaggcttg gcctatgaag ctgaagtgcc agtaaactgg
     9841 gttgaggaat tgggaactgc cattgccaat gaagaagtgc ttcctgacgg aacttctgag
     9901 cgtggaggct atccagttgt ccgcaaacca atgcgccaat ggatgctcaa aatcacggct
     9961 tacgcagagc gcttgctcaa tgacttagat gaactagatt ggtcagagtc tatcaaggat
    10021 atgcaacgca actggattgg taaatcaact ggtgccaatg taactttcaa agtaaaagga
    10081 acagacaagg aatttacagt ctttactact cgtccggaca cacttttcgg tgcgactttc
    10141 actgtcttgg ctcctgaaca tgaattagta gacgctatca caagttcaga gcaagcagaa
    10201 gctgtagcag actataaaca ccaagccagc cttaagtctg acttggttcg tacagacctt
    10261 gctaaggaaa aaactggtgt ttggacaggt gcctatgtca tcaaccctgt caatggtaag
    10321 gaaatgccaa tctggattgc ggactatgtt cttgctagtt atggaacagg tgcggttatg
    10381 gctgtgcctg cccacgacca acgtgactgg gaatttgcca aacaatttga ccttccaatc
    10441 gtcgaagtac ttgaaggtgg aaatgtcgaa gaagctgcct acacagagga tggcctgcat
    10501 gtcaattcag acttcctaga tggattgaac aaagaagacg ctattgccaa gattgtggct
    10561 tgcttggaag aaaaaggctg tggtcaggag aaggttacct accgcctccg cgactggctc
    10621 tttagccgtc aacgttactg gggtgagcca attccaatca ttcattggga agatggaact
    10681 tcaacagctg ttcctgaaac tgaattgccg cttgtcttgc ctgtaaccaa ggatatccgt
    10741 ccttcaggta ctggtgaaag tccactagct aacttgacag attggcttga agtgactcgt
    10801 gaagatggtg tcaaaggtcg tcgtgaaact aacaccatgc cacaatgggc tggttcaagc
    10861 tggtactacc tccgctatat tgacccgcac aatactgaga aattggctga tgaggacctc
    10921 ctcaaacaat ggttgccagt agatatctac gtgggtggtg cggaacatgc tgtacttcac
    10981 ttgctttatg ctcgtttctg gcataaattc ctctatgacc tcggtgttgt tccgactaag
    11041 gaaccattcc aaaaactctt taaccaaggg atgattttgg gaacaagcta ccgtgaccac
    11101 cgtggtgctc ttgtggcaac cgacaaggtt gaaaaacgtg atggttcctt ctttcatata
    11161 gaaacagggg aagagttgga gcaagcgcca gccaagatgt ctaaatcgct caagaacgtt
    11221 gttaacccag atgatgtggt ggaacaatac ggtgccgata cccttcgtgt ttatgaaatg
    11281 tttatgggac cactcgatgc ttcgattgct tggtcagaag aaggtttgga aggaagccgt
    11341 aagttccttg accgagttta ccgtttgatt acaagtaaag aaatccttgc ggaaaacaat
    11401 ggtgctcttg acaaggctta caacgaaaca gtcaaagctg ttactgagca aattgagtct
    11461 ctcaaattca acacagctat tgcccaactt atggtctttg tcaatgctgc taacaaggaa
    11521 gataagcttt atgttgacta tgccaaaggc tttattcaat tgattgcacc atttgcacct
    11581 cacttggcag aagaactctg gcaaacagtc gcagaaacag gtgagtcaat ctcttatgta
    11641 gcttggccaa cttgggacga aagtaaactg gttgaagacg aaatcgaaat cgttgtccaa
    11701 atcaaaggta aagtccgtgc caaactcatg gtcgctaaag acctatcacg tgaagaattg
    11761 caagaaattg ctctagcgga tgaaaaagtc aaagcagaaa ttgacggtaa ggaaatcgtg
    11821 aaagtgatta gtgtaccaaa taaattggtt aatattgttg taaaataacc aatttatttg
    11881 ctgatttcca acctcaaaca gtttcccaaa ctgtttgagc caactaaact cgttaatatc
    11941 gttgtgaaat aaggtaggaa tctttcagag tagaatctgg aggatttttt gaatcttctt
    12001 atgaaagtat ggtatactat gggcaactat aaagtttgaa aagtgaaaaa aggagaaagc
    12061 tatgccagta aatgaatatg gtcaaatgat tggggagtca atggaaggtt atacaccagg
    12121 tgaactgcct tctattgatt tcttagaagg gcgttatgct aggatagagg ctctttcagt
    12181 ggaaaagcat gcggaggatt tgctagctgt ttatggcccg gatactcctc gggagatgtg
    12241 gacctacctt tttcaggagc cagtagcaga tatgggggag ctggttagcc ttttacatca
    12301 gatgttggct cgtaaggacc gtttttacta tgcaatcata gacaaggcga ctggtaaggc
    12361 tttgggatct ttttctctca tgcgcattga ccagaataac cgagtaatag aagtcggagc
    12421 agtcactttt tctccagagc taaggggtac acggatagga actgaagctc agtatctcct
    12481 agctcgctat gtttttgagg agctaaacta tcgtcgctac gagtggaaat gcgatgcctt
    12541 aaatctgcca tcaagacgag ctgcggagcg tttgagcttt atttatgaag gaaccttccg
    12601 tcaggcagtg gtttataagg gacgtacgag agatacggat tggttgtcta tgattgataa
    12661 ggactggcct caagtcaaag atcgtttgga aacatggttg cgtcctgaaa attttgataa
    12721 aaatggacag cagtacaaga gcttgagaga actttaagag gtgttgagat gattactatt
    12781 aaaaagcaag aaattgtcaa gctagaggat gttttacatc tctatcaggc tgtcggttgg
    12841 acaaattata cccatcaacc agagatgctg gagcaggcct tatctcattc attagtaatt
    12901 tatctggcac ttgatggtga tgctgtggtg ggcttgattc gtttggttgg agatggattc
    12961 tcatcagtat tggttcagga tttaatcgtt ttaccaatct atcagcgtca aggaattggt
    13021 agtgccttga tgaaagaggc tttagaggat tacaaagatg cctatcaagt ccagctggtg
    13081 acagaagaga cagaaagaac cttgggcttc tatcgttcta tgggctttga aatcttatcc
    13141 acctataatt gtatagggat gacttggatg aatcgtaaaa aataacaaaa cttgtttttt
    13201 cttaagcaaa atttaa
//
LOCUS       DAVMEI010000044        13149 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVMEI010000044 DAVMEI010000000
VERSION     DAVMEI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.044"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..381
                     /locus_tag="VYQ24_000975"
     CDS             <1..381
                     /locus_tag="VYQ24_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1968131.1"
                     /translation="NLSTSPNLEQVQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHR
                     FLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDYIDY
                     YNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(509..712)
                     /locus_tag="VYQ24_000976"
     CDS             complement(509..712)
                     /locus_tag="VYQ24_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968132.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(705..1787)
                     /locus_tag="VYQ24_000977"
     CDS             complement(705..1787)
                     /locus_tag="VYQ24_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1968133.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1913..2695)
                     /locus_tag="VYQ24_000978"
     CDS             complement(1913..2695)
                     /locus_tag="VYQ24_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1968134.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2695..4038)
                     /locus_tag="VYQ24_000979"
     CDS             complement(2695..4038)
                     /locus_tag="VYQ24_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1968135.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNSIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4137..5453)
                     /locus_tag="VYQ24_000980"
     CDS             complement(4137..5453)
                     /locus_tag="VYQ24_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1968136.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5646..6872
                     /locus_tag="VYQ24_000981"
     CDS             5646..6872
                     /locus_tag="VYQ24_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1968137.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7071..8645
                     /locus_tag="VYQ24_000982"
     CDS             7071..8645
                     /locus_tag="VYQ24_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1968138.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRIEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8910..9698
                     /gene="codY"
                     /locus_tag="VYQ24_000983"
     CDS             8910..9698
                     /gene="codY"
                     /locus_tag="VYQ24_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1968139.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9698..10273
                     /locus_tag="VYQ24_000984"
     CDS             9698..10273
                     /locus_tag="VYQ24_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1968140.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGNLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10355..10440
                     /locus_tag="VYQ24_000985"
     tRNA            10355..10440
                     /locus_tag="VYQ24_000985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10389..10391,aa:Leu,seq:aag)
     gene            10723..11853
                     /gene="ugpC"
                     /locus_tag="VYQ24_000986"
     CDS             10723..11853
                     /gene="ugpC"
                     /locus_tag="VYQ24_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1968141.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(11976..12146)
                     /locus_tag="VYQ24_000987"
     CDS             complement(11976..12146)
                     /locus_tag="VYQ24_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968142.1"
                     /translation="MEIKEQTRKLAVSYSKYSFEVADKTDEVSNHTYGKATLTWFEEI
                     FEEYKEHHNIDV"
     gene            12354..13112
                     /locus_tag="VYQ24_000988"
     CDS             12354..13112
                     /locus_tag="VYQ24_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1968143.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
BASE COUNT         3912 a         2419 c         2874 g         3944 t
ORIGIN      
        1 aatctttcaa cttcacccaa cttagaacaa gtacaaacaa tgttggaaca ggcattcaaa
       61 gagaagcact acgagaatac gattctccat agtgaccaag gctggcaata tcaacacgat
      121 tcttatcatc ggttcctaga gagtaaggga attcaagcat ctatgtcacg taaaggtaac
      181 agcccagaca acggtatgat ggagtccttc tttggcattc tgaaatcgga gatgttttat
      241 ggttatgaga agtcatttca gtcgcttaag caattggaac aagccattgt agactatatt
      301 gattactaca acaataagag aattaaggta aaactaaaag gacttagccc tgtgcaatac
      361 agaactaaat ccttcggata aattatttgt ctaacttttg gggggcagta cattttaaca
      421 cttgatacta tgcgttttat tgtgggaaga ttttcctcat tttctagtga aattgaactt
      481 tttctaacat cttttgatag atgacttctt aatcaattat catttccatt gttgtttctt
      541 ggggtttcat cccttgtctt tgataaaagc ggagagcacc ctcgttatca ttccagacac
      601 ttagggttag attattgcag ccaatttctt tagcataata gcagaaaaat gacaataaaa
      661 tagcccctag atattttgaa aggaaagcat aggggctata tagtttaatc aaaataaagc
      721 aaatctttgc tggtgcttgt aaagaggtcg aagccatcct tggtaacaac accgcagtct
      781 tcaatacgaa caccgacttt accagggata tagatacctg gttcaacaga gaagcacatg
      841 ccttcttcga tgaccatgtc gtttccttcc atgatagatg ggaattcatg gacatccata
      901 ccgataccat gcccgagacg gtggttgaag tactcaccat aaccagcttt ttcgatgacc
      961 tcacgggcag cgcggtccac ttcatgagca gtcacacctg gcttgataaa gtcaagagca
     1021 gcttgttggg cttcaagagt caagttgtaa atatctttct tgaattggtc tggtttgccg
     1081 acagcgactg tacgagtcat atctgacgca tagccattga ccagaacacc caggtcaaag
     1141 aggagaagag catcattttc aaccttatta gctgctggaa tgccgtgtgg attcgcagca
     1201 ttatcaccag tcaagaccat ggtatcaaag ctcatttcat aaccttcacg tttcatggca
     1261 aagtcgattt gtgcgatgat atctgtctca gtcttatcaa gagaaatatt gtcaaaacca
     1321 acatgaacag ccttgtcagc ataaagacct gcaaccatca ttttttgcac ttcatcagct
     1381 gatttgatga ggcgcatgcg ttggatacga ggagtgaggt tgtcaaactc agcagtctca
     1441 aaaactgttt tcaaaccatg gtatttggtc aagatgagat tgtcaaactc aacagcgaca
     1501 cgtttgaagt caagttgtgg aagagcatgt ttgatttttt gccatggatt ttcagaatcg
     1561 acatagccca ctactgggaa ggaaacggtg ctacttgcac gttctacttc aagagctggg
     1621 acaaagagga gaggttcctg atccgctagg acaaagagga acatttggcg ttcatgggga
     1681 tcactgtaaa aaccagtgag gtaattgatt gtgacggggt cagatacgac agcgacgtct
     1741 agtttttctg attcaagata tgttaggatt tgttgtaatt tagacatatg ctaccttctt
     1801 tctacccctc tattttggca aaaaatgaga gaaaatgcaa gggagaaggg taaaagaaaa
     1861 gacaaaaaac tccgacaaga taacggagtt ctttgatatc attttccttt gtttaagaaa
     1921 agtcagcctt gcttttgacg tcactgtact cttcagcgat ttcttggctg agaatgtcct
     1981 cataggcagg gagttggatg tcctgaccat atttcttctt gagggcagta gtaactaggc
     2041 gataagccag attggcatta cgagagagga taggcccgtg gaagtaggaa ccaaagacat
     2101 tcttataatg aaccccttca ccgacctttt cttcgttgtt tccatttcca tagacaacct
     2161 gtcccagcgg tttttggtca tcagagagga aggtacgacc ttggtgattt tcaaatccat
     2221 agtaggtttc atcgaaatct tcattgtgaa tcttgatgtc accgataaaa cggttattgg
     2281 tctggttgag cgtgtagtgt cccatgaccc ctagcccttc gatacgtttt cctgaagctt
     2341 caacataata ttgacccaat agttggaaac caccgcagat agccagaact acaccgtcgt
     2401 tttggatgta gttgtcaatg ctctcttttt tagcaggtag gtcgtctgca atgatacttt
     2461 gttcaaagtc ttgaccacca ccgaaaaagg cgatgtcgta gtgattttca tcaaagtcat
     2521 catggagaga aacgatgtca acggtcacat gggctcccag tttttcagcc acatacttga
     2581 gcatgaggat gtttccattg tccccgtagg tattcatgag atttccgtag aggtgggcaa
     2641 tgttgagctg ataggggtaa ttgctatctt ttgaggaaag tgaagtataa accattagtt
     2701 catctccttt ctaacaatct gacgactagc cagcagttca cgaaattcca gcatggcagt
     2761 ataagttgcc agaatatagg catgcttgca gtcttgattc tcaatggtct tgagaacttg
     2821 ctccagatta ctcgtttcag tgattttctc agctggatag ccagtcactc ggaggcgacg
     2881 agcgatttca gaatgacgaa caccgccagc gttgatttca ggaatgtcca tgtcagtgat
     2941 ttgctcaaag tctgcatccc agatccagct agtgtcaatt ccatctgcat agttggcatt
     3001 aaggaggaca gataggctaa atggataagg tgctagtttg atcatttcga tagcttgggt
     3061 tgcaccgact ggatttttaa tcaagacaag ggtacattcc ttgtcaccga tatgaaaggt
     3121 ttcttggcgt ccaaagacag cacggctctt gtcaaatccc tgtttgatga gttgggaatc
     3181 tgcgccgagg aaacgggcga tggccacagc agctagggcg ttatagatat tatagagccc
     3241 gccgatttgg ataccgtatt cttggccgtc tatgacaaag cgagagcgat tgttggtcaa
     3301 ctcaaccagt tttgtcaaac gatagtcgag atcaggacgt ttacatccac aaccttcaca
     3361 gatataggca cccaagtttg cataggtatt atgctcatat ttgaggatgc cttggcagtc
     3421 aggacagaga atcccttcgg tattgtagtg agcaagttgg gctggtcctt tttccaagtc
     3481 aaaaccaaaa tactctatag agtttggaat agttggcttg tagaaaagtg gactgtctcc
     3541 gttaaggaga acagtagcag ttggaacttt tcgaatggca tccaatatca tgttataggt
     3601 agtatagatt tcaccgaaac ggtccatctg gtcacggaag atattagtaa tgacaaaaag
     3661 actaggctgg atatagtcac agatacgaga tagactggct tcgtcaattt cgaggacggc
     3721 aatatttttc ccagtttttg aagatttggc tgttaggaag gttgttgcaa tccctgtaat
     3781 catgttggca ccgcttgggt tggttagaac ttgaccataa acctctttta aaatgccgac
     3841 agtgagggca gttgtcaggg tttttccatt tgttccagtg acaacgacaa tctcgtagtt
     3901 cttagctagg ctttgtaaaa tatctttatc aaattgaagg gcgactttcc ctgggagcgt
     3961 acttccacgt ccaagacggc ttaaaacgaa gtgggaagaa cgcccagcaa gaaggcccaa
     4021 agtagttttt aagttcatgt ttctattata tcacaaaaga gggaaaagac agatttggtg
     4081 tttcgtagaa gtaaaaacag cccaaagagg gctgtcagtt aagatgagat ttcaacttaa
     4141 atcgcaaaca agtcattcaa gttctcagcc aaggttgggt gagtgaagat ttgttttgtg
     4201 aagtaagtgt aaggaatctt gttgtccata gcaacagtaa tgatgttgat gatttcttga
     4261 gaaccttctg agaagatgct tgcaccaaga atttcttttg tttcagtatt aacaacagct
     4321 ttgaaagctc cgcgaaggtc tccatttacg tgaccacgag gcatggctgc aacagggatt
     4381 tctttcactg cgtatggaag tttcaaatca gctgcttggc tttcagtcaa accaacttgt
     4441 gaaagtgcag gtgtgatgaa catagtattt ggcacattga gacggtcctc aagtgtgtag
     4501 ctgccatctc cagcaaggta gctgtaaaca acacggaagt catcaagtga aatgtaagta
     4561 aattgaaggc caccgttgac atctccaact gcaaagacac caggaacgtt tgtttgacag
     4621 tgtttgtcta ctttaatagc accacgttca gttagttcaa tatctgtatt ttcaagttga
     4681 agtggttcta catttggttt gcgtccagtt gcgtagagaa gggcgtcgaa acggtaagtt
     4741 tcgtcttcag ttacgacaag cacttggtca ccatcgtttt tgatttcagt agtatggata
     4801 ttttgaagca attcaatgcc atcttcttcc atgtattgtt tagcaagagc tgcgatggaa
     4861 ggttctgcac gaggtaggaa tgtatccaag gcatctagga ctgtgacctt gcttccaagt
     4921 ttgttgtaaa ggccggcaaa ttcaagaccg atatttccgc caccaaggat tccaagtttt
     4981 tcaggtaatt tgtccaagct ttggatacct gttgagtcaa agatgttttt gcttgtagca
     5041 agtccaggaa ttggcaagac gtttgaaaca gcaccagtgt tgatgacgat tgtttcagca
     5101 gtcagttctt tcttttcatc accagcttgg atttcgatga ctttatttga aaggaagtga
     5161 gcttccgcat caaagatatc tacgcctgta ccagcaacag tcgcatagtt tttaccgttg
     5221 aggcgaccag tgatcgtgtt tttagtagca atcacttctt caaaagacaa gtccttttca
     5281 gcagcaacta gcaaggtttt agttgggata caaccaatgt tgatacaagt tccaccgtac
     5341 atagccttgc tgcgttcaac gagggcaact tttttgccag ctgaagccaa tttacctgct
     5401 agtgttttac cagctttacc aaatccaata acaattaaat cgtatgttaa catttatctt
     5461 cctcctattc gaatttcatt ctagcacaag aaaaaaactt ttgcacaatt ctgctacgga
     5521 aaagacagaa aaagataaaa acgctttcca gacaaaaaac tagtaatttt ttgacaagct
     5581 tgataaacta aaatcctatc tatttttata taagaaagaa taaatgttcg aaaataggag
     5641 gttttatgaa atcgaatcgt tatattattg cctttgctgg ggtcatttta cacttaatgc
     5701 tgggttcgac ttatgcttgg agtgtttatc gtaaccctat tattgaaaaa acgggatggg
     5761 atcaggcttc tgttgccttc gcctttagtc tagcaatctt ttgtttgggc ttatcggctg
     5821 catttatggg gcgtttggta gaaaaatttg gtccgaaagt catggggagt ctatctgctt
     5881 ttctatacgc aggtggaaat atcttaacag gatttgcaat agaccgtcag gaactgtggt
     5941 tgttgtatct agcttatggc attttaggtg ggcttggttt gggagcaggc tatattaccc
     6001 ctgtgtcaac gattataaaa tggtttcctg ataaacgtgg tctcgcaaca ggtttagcga
     6061 ttatggggtt tggttttgct tctttattga ctagtcccat agcgcaacac ctcatcgcag
     6121 gggtagggat tgtagaaact ttttatattt taggagcaag ttactttatt atcatgctcc
     6181 tagcttcaca attcattaag cgtccaaatg agcaagagct tgcaatttta tcttcttcag
     6241 ggaaagaaaa aacagcctct ttgacgcaag gaatggctgc aaatcaggct ctaaaaagca
     6301 atcggtttta tatgctttgg attattttct ttatcaacat agcttgtggt ttaggcttaa
     6361 tttcagcggc atcgccaatg gcacaggaga tggctggctt gtctacaagt catgcagcag
     6421 taatggtggg tgttttgggg attttcaatg gatttggtcg cttgctctgg gcgagtttgt
     6481 ctgactatat cggtcgccct ctaaccttta gtatattact gcttgtcaat cttttctttt
     6541 ctctctcact ttggctcttt acagattccg ttttatttgt agttgctatg tctattttga
     6601 tgacttgcta tggagctggt ttttctttga ttccagctta tctcagtgat atttttggaa
     6661 ccaaggaatt ggccgctctg catggttata ttttaacagc ttgggcaatg gctggtttag
     6721 cgggacctat tttattagca gagacttata aaatggctca ttcgtacaca caaaccttgt
     6781 tcgtttttct cattttatac agtatcgcct tggctttgtc ttattatcta ggtcgttcaa
     6841 tcaaaaaaga aagtcaaaaa ccgcttacat gattttctgt agaaatgggg ctatcttttg
     6901 ctctggtctt gtgttatact agataggttg caaagaaaac agtacttttc ttttgtggaa
     6961 aagaagcaac acgattttat atctagttat atgaaaatac gtcataaaaa gaaaagtata
     7021 aactaagccc tatagggtgg cttcgcacca cctttagaaa gaagaataac gtgaaattta
     7081 atgaattaaa cttgtctgct gatttgctag cagaaattga aaaagctggt tttgtagaag
     7141 ctagtcctat ccaagaacaa actattcctt tggcccttga aggcaaggat gttatcggtc
     7201 aagctcagac tggtacagga aaaactgcag cctttggctt gcctaccctt gaaaaaatcc
     7261 gtatagaaga agcgactatt caagccttgg tcatcgctcc aactcgtgaa ctagctgtcc
     7321 aaagtcaaga agaactcttc cgctttggtc gtagtaaggg agtcaaagtc cgttcagtat
     7381 atggcggatc aagcattgaa aaacaaatta aggctcttaa atctggtgcc catatcgtgg
     7441 tgggaactcc aggtcgcctc ttggacttga ttaaacgcaa ggccttgaaa ttacaagaca
     7501 ttgaaaccct tatccttgac gaagcggatg aaatgcttaa catgggcttc cttgaagaca
     7561 tcgaagccat tatttcccgt gtacctgaga accgtcaaac tttgcttttc tcagcaacta
     7621 tgccagatgc catcaaacgt atcggtgttc agtttatgaa agcccctgaa catgtcaaga
     7681 ttgcggctaa ggaattgaca acagaattgg ttgaccagta ctatatccgt gttaaggaac
     7741 aagaaaaatt tgacaccatg actcgtctca tggatgtggc acaaccagaa ctcgctattg
     7801 tatttggtcg taccaaacgc cgtgtggatg aattgactcg tggtttgaaa attcgtggct
     7861 tccgtgcaga aggaattcat ggcgacctag accaaaacaa acgtcttcgt gtccttcgtg
     7921 actttaaaaa tggcaatctt gatgttttgg ttgcgacaga cgttgcagcg cgtggtttgg
     7981 atatttcagg tgtgacccat gtctacaact acgatattcc acaagatcct gagagttatg
     8041 ttcaccgtat cggtcgtaca ggtcgtgctg gtaagtcagg tcaatctatt acttttgttg
     8101 ctccaaacga aatgggttac cttcaaatca ttgaaaactt gactaagaaa cgcatgaaag
     8161 gtctcaaacc tgcaagtgta gaagaatcct tccaatcaaa aaaacaggta gctctcaaga
     8221 aaatcgaacg tgattttgca gatgaaacca ttcgtgccaa ctttgagaaa tttggtaagg
     8281 atgctcgcaa attggctgct gagtttactc cagaagaatt ggcaatgtat atcttgagtc
     8341 tgacagtcca agacccagat agccttccag aagtggagat tgcacgtgaa aaaccactac
     8401 cgtttaaacc atcaggtaat ggtttcggtg gtaaagctaa gggaggtcgt ggaggccgtc
     8461 gtggggacga ccgtcgagag cgtgatcgtc gtggcaatgg tcgccgtgat gagttcaaaa
     8521 aaggaagtcg tggcaacgat cgttttgata aggaaaaacg ttaccgtaag gataataaaa
     8581 aaccacgcaa tactttaagc gaaaagcaaa caggctttgt tattcgtaac aaaggtgata
     8641 aataagaaaa agcggttcaa aatgaatcgc ttttttatat aaggagaccg aatgtgaaaa
     8701 agacaaataa aattattata ttatctttta taatattatg atacacaaaa ataggcgact
     8761 tgtcaataga aaaggaacaa tttaattaaa aatattctgg tttttaatta aattgtaagt
     8821 caatgcaagt caataaggaa ttttcagata attattgaaa aagagacgtt tttatgttat
     8881 aatgatagcg attaaattcg aggtgaaaaa tggcacattt attagaaaaa actagaaaaa
     8941 ttacatcaat tttgaaacgc tcagaggagc agttgcagga tgagctccct tacaacgcta
     9001 ttacgcgtca gttagcggat attattcatt gcaatgcctg cattatcaat agtaagggac
     9061 gtctgcttgg ctattttatg cgttataaaa caaatacaga tcgcgtagag caattcttcc
     9121 aaactaagat tttcccagat gactacgttc aaggggctaa tatgatttac gaaacagaag
     9181 caaacttgcc tgttgagcat gatatgagta ttttccctgt tgagagtaga gatgattttc
     9241 cagatggctt gacgactatt gcaccgattc atgtatcggg gattcgcctt ggttctttga
     9301 ttatttggcg taatgataaa aaattcgaag atgaggactt ggttcttgtt gagattgcca
     9361 gtaccgttgt tgggattcag cttcttaact tccaacgtga agaagatgag aaaaatattc
     9421 gtcgtcgtac tgctgtcacc atggcggtca ataccctttc ttactccgaa ctccgtgctg
     9481 tttcagcaat tttaggggaa ttaaatggaa atgaagggaa gttgactgcg tcagtgattg
     9541 cagatcgtat cggaatcact cgctctgtga ttgtcaatgc tcttcgtaaa cttgagtctg
     9601 cggggattat tgaaagtcgc tcacttggaa tgaagggaac ctatcttaag gtcttgattt
     9661 cagatatttt tgaagaagtg aagaaaagag attactaatg acaaaggctt taatttcgat
     9721 tgattataca gaagattttg ttgctgatag tgggaagctg acagcaggtg ctccagctca
     9781 ggcgatttcg gatgccatca gcaaggtaac tcgattagct tttgaacgag gagattatat
     9841 cttctttact attgatgctc atgaagaaaa cgattgtttc catccggaaa gtaagttatt
     9901 tcctcctcat aatctgattg ggacgagtgg acggaattta tatggaaatt tggggatctt
     9961 ttatcaagag catggttcag acagtcgtgt cttttggatg gataaacgcc attactcagc
    10021 tttttcaggg actgacctag atattcgttt gagagagcgt agagtgtcta ccgttatctt
    10081 aacaggtgtc ttgacggata tctgtgtcct acatacagct atagatgcct ataatctagg
    10141 atatgacatc gagattgtta aaccagctgt tgcttccatc tggcctgaaa atcatcaatt
    10201 tgccctaggt catttcaaaa atacactcgg agctaagtta gtagatgaaa atctaaatga
    10261 actttctgag taacctggtt ggtaaaaatg aaaaaatttg aaaaaaagtg ttgacaagca
    10321 tcccaaaagt tgatatacta gtaaagtaat cgatgcgggg atggcggaat tggcagacgc
    10381 gcaggactaa ggatcctgtg accgctttag gtcgtgaggg ttcaagtccc tctctccgca
    10441 taggaaaaga gttagctaaa gctagctctt ttgtatttct ctaaaaaatt agaatccagt
    10501 caaatgtagt ggagaatcag cgaaaatatg ggaaaactga tccttgtttc tgcttttgtc
    10561 atagaagaat aataaagatt atcttcttca aattctccga tattctctaa agttttgtgc
    10621 aagttgcaca gaactttttt atttttttgg tcatcttgcc atagaaatat aaagcgtttt
    10681 catctataat ataattatca aaagacaaaa ggagttcacc tcatggtaga attgaatctt
    10741 aaaaatattt acaaaaaata tccaaacagc gaacactatt cagttgaaga tttcaacttg
    10801 aacatcaaag ataaagaatt tatcgttttc gtaggacctt caggatgtgg taaatcaact
    10861 acactccgta tgattgctgg tcttgaagac atcacagaag gtactgcatc tatcgatggc
    10921 gtggttgtca acgacgtagc tccaaaagac cgtgatatcg ccatggtatt ccaaaactac
    10981 gctctttacc cacacatgac tgtttatgac aacatggctt tcggtttgaa attgcgtaaa
    11041 tacagcaaag aagacattaa caaacgtgtt caagaagcag ctgaaatact tggattgaaa
    11101 gaattcttgg aacgtaaacc ggctgacctt tcaggtggtc aacgtcaacg tgttgccatg
    11161 gggcgtgcga ttgtccgtga tgcgaaagta ttcttgatgg acgaaccttt gtcaaacttg
    11221 gatgccaaac ttcgtgtatc aatgcgtgct gaaatcgcta aaattcaccg tcgtatcgga
    11281 gctacaacta tctatgtaac tcacgaccaa acagaagcga tgacacttgc agaccgtatc
    11341 gttattatgt cagctactaa gaaccctgct ggtacaggta ctatcggacg tgtagaacaa
    11401 atcggtactc ctcaagaagt ttacaaaaat ccagttaaca aattcgttgc aggattcatc
    11461 ggaagcccag ctatgaactt catcaacgtg aaattggttg gtagcgaaat tgtttctgac
    11521 ggtttccgtt tgaaagtgcc agaaggagca ttgaaagttc ttcgtgaaaa aggctacgaa
    11581 ggaaaagaat tgatctttgg tatccgtcca gaagacgtga atgcagaacc tgctttcctt
    11641 gaaacattcc cagactgtgt tgtaaaagcg actatctctg tatcagaact gcttggttca
    11701 gaatctcacc tttactgcca agttggtaaa gacgagtttg ttgcaaaagt tgatgctcgt
    11761 gactacttgc aaacaggtgc aacagttgag cttggatttg acttgaacaa agcacacttc
    11821 ttcgatgtag aaactgaaaa aacaatctac taagaataaa taaaattcaa agcactacaa
    11881 gaaaagatat ctctttatca attgtagtgg agagatatca gttaatctag ggagagaaac
    11941 aaaatgcttc tctccttttt gctagggaag tcatattata catctatatt gtgatgctct
    12001 ttatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta cgtatgatta
    12061 ctgacttcgt cagttttatc tgcaacctca aagctgtact ttgagtagct tacggctagt
    12121 ttcctagttt gctctttgat ttccattgag tattatttgt gggtaccatc tacaagtgaa
    12181 gctatatgcg taaactacgt gagcaattga attcgaacta gagaggtaat aataaattta
    12241 tgctatagtt atggtgactt gtatgctttt gattctagtt tatcaaataa tagattagaa
    12301 ttgtcagata atatcatttt gtgttataat gaagaaaaaa cagaggtgtt caaatgtcag
    12361 aagcaggtca taagttttta gcaaaattgg ggaaaaaacg cttacgtcca ggtggaaagc
    12421 gtgccacaga ttggttaatt gcagaaggag gattttcaaa agaaaagaga atactagagg
    12481 ttgcgtgtaa taggggaact acagcaattg agttggcaca gcgttttggt tgcaagataa
    12541 ctgctgttga tatggatgct caagctttag aagtggctaa aaaatctgct ggaacggcag
    12601 gtgttgctca tttaatcagt tttgaaagag caaatgcaat gaaacttcct tatcaagatg
    12661 ctagttttga tattgttata aatgaagcta tgctgactat gcaagccgat caagctaaga
    12721 aaaaatgtgt aatggaatat ctaagggtat taaaacctgg aggtcttctc ttgacacatg
    12781 atgtacttct taaggaagct aaagagtcta tcagacagga attatcacaa gcaattcatg
    12841 taaatgtagg tcctttaact caagatggtt gggaacaggt gatgatagaa tcaggttatt
    12901 gtgatgtgaa agcattgact ggtgaaatga cattaatgaa attatcgggt atgatttatg
    12961 acgaaggttt gctaggaact ttgaaaattt gtgtaaatgc ttgtaaaaag gagaatagaa
    13021 agcagttttt aactatgtat aaaatgtttg ctaagaataa acagaaattg ggctttattg
    13081 cgatggctag ttataaatcg tcaaaacgtt agatagttat tgaagttaac ttttcctttt
    13141 ttctttctt
//
LOCUS       DAVMEI010000045        12787 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVMEI010000045 DAVMEI010000000
VERSION     DAVMEI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.045"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            80..514
                     /locus_tag="VYQ24_000989"
     CDS             80..514
                     /locus_tag="VYQ24_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1968144.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            526..1716
                     /locus_tag="VYQ24_000990"
     CDS             526..1716
                     /locus_tag="VYQ24_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEV1968145.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANNQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            1727..2218
                     /locus_tag="VYQ24_000991"
     CDS             1727..2218
                     /locus_tag="VYQ24_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1968146.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELNQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            2233..3012
                     /locus_tag="VYQ24_000992"
     CDS             2233..3012
                     /locus_tag="VYQ24_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1968147.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            2999..3817
                     /locus_tag="VYQ24_000993"
     CDS             2999..3817
                     /locus_tag="VYQ24_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1968148.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            3817..4110
                     /locus_tag="VYQ24_000994"
     CDS             3817..4110
                     /locus_tag="VYQ24_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1968149.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIQDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            4132..6033
                     /locus_tag="VYQ24_000995"
     CDS             4132..6033
                     /locus_tag="VYQ24_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1968150.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLIDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKGYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            6093..7094
                     /locus_tag="VYQ24_000996"
     CDS             6093..7094
                     /locus_tag="VYQ24_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1968151.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(7204..7539)
                     /locus_tag="VYQ24_000997"
     CDS             complement(7204..7539)
                     /locus_tag="VYQ24_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968152.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(7550..7792)
                     /locus_tag="VYQ24_000998"
     CDS             complement(7550..7792)
                     /locus_tag="VYQ24_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968153.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(7808..8002)
                     /locus_tag="VYQ24_000999"
     CDS             complement(7808..8002)
                     /locus_tag="VYQ24_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968154.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            8168..9118
                     /gene="rsmH"
                     /locus_tag="VYQ24_001000"
     CDS             8168..9118
                     /gene="rsmH"
                     /locus_tag="VYQ24_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1968155.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLRETGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            9130..9447
                     /gene="ftsL"
                     /locus_tag="VYQ24_001001"
     CDS             9130..9447
                     /gene="ftsL"
                     /locus_tag="VYQ24_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1968156.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            9451..11703
                     /locus_tag="VYQ24_001002"
     CDS             9451..11703
                     /locus_tag="VYQ24_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEV1968157.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGSKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRVGNIVPGTELVSQQTVDGKDVYTTLSSPLQSFMETQ
                     MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATTRDWDVNDGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGNPVSKEAA
                     STTRNHMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSVVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIKN
                     IKKIKLTLGD"
     gene            11705..12685
                     /locus_tag="VYQ24_001003"
     CDS             11705..12685
                     /locus_tag="VYQ24_001003"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1968158.1"
                     /translation="MFISISAGVVTFLITLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIAAVVVSFLLALFSKQLTNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFSYQVHLGIFYIIFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
BASE COUNT         3901 a         2240 c         2875 g         3771 t
ORIGIN      
        1 ctattgacaa gataaaaata aaggattaca atggggaata taaagtaaac cggtaaacct
       61 aaacaagaaa ggagaaaaga tgaaaattgt acttgtaggg catggacatt ttgctacagg
      121 gatttatagt tctttacaat tgattgcagg caatcaagaa aatgtggagg caattgactt
      181 tgtggaagga atgtcagcag atgaactcaa gcaaaaaatc ttacttgcaa tttcaaatga
      241 agaagaagtt ttaatcctaa gtgatctctt gggaggatcg ccattcaagg tttcttctac
      301 cataatggga gaaaatccag ccaaaacaat gaatgttctt tcgggtttga acttagccat
      361 gttaatggaa gcagtctttg ctagaatggc tcatagcttt gatgaggttg ttaataaatc
      421 agtagtggcg gcccagggcg gagtcgtaaa tggtaaagaa ttgttttcaa cggatgcaga
      481 ggaagaggaa gaagatttcg aatcgggtat ttaaagggta aaagaatgat aaaaaaggtt
      541 acgattgaaa aaataaaatc gcctgagcgc ttcttagaag taccacttct gacgaaagaa
      601 gaagtcggcc aggcaatcga taaggttatt cggcagttag aactcaacct tgactatttc
      661 aaggaagatt tcccgacgcc agctaccttt gataatgtct atccaatcat ggataacacg
      721 gaatggacca atggtttctg gacaggagaa ctgtggttgg cttatgaata cagtcaacag
      781 gatgcattta aaaacatcgc tcataaaaat gttctttctt tcctggatcg tgtcaataag
      841 agagtagaat tggatcacca tgatctcggc ttcttgtaca caccgtcttg tatggctgaa
      901 tataagataa atggagatgg agaggctaga gaagcagcct tgaaagctgc agataagttg
      961 attgaacgct atcaagaaaa aggtggtttt attcaagctt ggggagactt gggcaagaaa
     1021 gagcattacc gtttgattat cgattgcttg ctcaatatcc aactcttatt ctttgcttat
     1081 caagaaacag gcgatcaaaa atactacgat attgcagaaa gccatttcta tgcttcagct
     1141 aataatgtaa tccgtgatga cgcttcgtcc ttccacacct tctattttga tcctgagaca
     1201 ggtcaaccct ttaaaggtgt aacgagacaa gggtatagtg atgattcatg ctgggcacgt
     1261 ggtcagtcat ggggagttta tggtattcct ttgacttatc gtcacttaaa agacgagtcc
     1321 tgctttgact tgtttaaggg tgtgaccaat tatttcttga atcgtctacc aaaagatcat
     1381 gtgtcctatt gggatttgat ttttaatgat ggtagtgatc aatcacgaga ttcttcagca
     1441 acagctatcg ccgtctgtgg gattcatgaa atgctaaaac atctcccaga ggtggatgct
     1501 gacaaagata tttataaaca tgctatgcat gccatgcttc gttccttgat cgaacattat
     1561 gcaaataatc aatttacccc tggtgggaca agtctcctcc acggtgtgta ctcatggcat
     1621 tcaggtaaag gagtggatga aggcaatatc tggggtgact actattacct agaagccctt
     1681 atccgtttct acaaagactg gaacctatat tggtaggagg agaaatatga caatgccaaa
     1741 tattattatg acccgtatcg atgaacggtt gattcatgga caaggacaac tttgggtaaa
     1801 atacctaggt tgtaatacgg tcattgttgc caatgacgaa gtaagcacgg acaagatgca
     1861 acaaactctg atgaaaacag ttgtgccaga ctcagttgcc atgcgtttct tccctttgca
     1921 aaaggtgatt gatatcattc acaaggctaa tcctgctcaa acgatcttta tcgttgtaaa
     1981 ggatgtgaag gacgctttaa ccttggtaga aggtggtgtc cctatcaaag aaatcaatat
     2041 tgggaacatt cacaatgcta ctggtaaaga gcaagtgaca cgctccatct tcctgggtga
     2101 agaggacaag gcggccctca aggaattgaa ccaaactcat caagtaacat ttaatacgaa
     2161 aacaactcca acaggaaatg atggagctgt tcaagtcaac attatggact atatttaaaa
     2221 gaggagatcg ttatgtcgat taatgtattt caagcgattt taattggatt atggacagct
     2281 ttctgtttta gtggaatgct gttaggaatt tacaccaata gatgcattgt tctgtcattt
     2341 ggtgtcggaa ttattctagg tgatctgcct actgctcttg caatgggagc tattggtgaa
     2401 ttggcttata tgggattcgg tgttggtgct ggaggtactg ttccaccaaa cccaatcgga
     2461 cctggtatct ttggtacctt gatggctatc actagtgctg gtaaagtcag tccagaagcg
     2521 gctcttgccc tctctactcc gattgctgtg gcgattcaat tcttacaaac tttcgcctac
     2581 actgtacgtg ctggtgcgcc tgaaacagcg atgaagcact tgaaaaacca taatttgaag
     2641 aaattcaagt tcactctaaa tgcaacaatt tggttgtttg cctttattgg atttaccttg
     2701 ggttgcttgg gtgccctttc aatggatacc ttgttgaaac tcgtagacta cattccaccg
     2761 gtattgctta caggtttgac agttgctggt aaaatgctcc cagctatcgg ttttgcgatg
     2821 atcttgtcag tgatggctaa gaaagagttg attccctttg tcttgttggg atatgtttgt
     2881 gcagcttatc taaacatccc aacaattggt attgcaattg taggtactat ctttgctttg
     2941 attgaatttt ataacaagcc aaaaacagcg gatcatgtgg tagaggagga agcacacgat
     3001 gactggatct aacaaattaa caaaacgtga ttatcttaaa acgtctttgc gggcattctt
     3061 ttgtcaaaat ggatttaact atagtaacta tcaagggttg ggatatgcca atgtgatgta
     3121 tcctgctttg aaaaaacact atggagagga tcaggaaggt ttctaccaag ccttggaaga
     3181 aaactgtgaa ttctataata ccaacccaca cttcctgcct tttattacca gcttgcatct
     3241 tgtaatgttg gaaaatggcc gtccggcaaa agaaacacgt agcatcaaga tggccttgat
     3301 gggaccattg gcaggtattg gggattctct ttctcaattc tgtttagctc cattgttctc
     3361 aaccatcgca gcttcgtttg ctcaagaagg cttggttgtc ggtccaatct tgttcttcct
     3421 tgcgatgaat acgattttaa cagcgattaa attgtcaact ggtctgtatg gatacaaact
     3481 aggaacaact gtgattgata aactaagcga acagatggca acgatttctc gtattgccaa
     3541 tattatcggt gtaaccgtaa ttgctggttt ggcagcgaca tctgttaaga ttatggtgcc
     3601 gattaccttt gctgcagggg aagttaaagc agacgctaaa caaagtatcg taagtattca
     3661 gggaatgctt gataaggttg ctccagctct tctaccagcc ctatttacac ttttagttta
     3721 ctacttgatc aaagaaaaga aatggacaac atataaactc gttattttaa cagttatcat
     3781 cggaattatc ggaagctggc ttaagattat agcttaatgg atacaacatt gttttatgga
     3841 atagtgattg tcttggcagt gagtcccctt ttactgtcaa gctttcattc tattcgtcag
     3901 caaaagttgc ttcgcaaaca gatggagcaa cgacaagagt atttagcttc tttaacatct
     3961 ggtgatgaag tgttgttatt gtctggaatt catggaaaaa tcatttctat ccaagatgac
     4021 ttggtctcct tgcagattgc aaaaggtgtg gtcatctatg tagaaaagga aagtgtaatg
     4081 ggaaagacaa aagaactgct ttttaagtag ttctttttct ggtaatagat aatgaaagaa
     4141 gaaagaagac aattttttga aagagtcgat ggaaaccaat gtcgtgatta tatcttgtcc
     4201 cactgttcaa aagactatga gaaagtcaag agttcccttg aacgcttgat agacaatcgt
     4261 tttatgtttg atagtccttg ggatatggag ccttgttcaa aaatccatca aatccagccg
     4321 atggtatggg atcaagtatt tgaagatgat ccagaatggt cttatatgct caatcgacaa
     4381 gaatatctct tgcagtttat gatagggtat ctggtagaag gagataaggg ctatatccaa
     4441 aagtgcaagt tctttctatt tgattggatt gagcaggtga gagaattttc tcctcaatcc
     4501 ttgatgacta gaaccttgga tacgggtatt cgttccttta cttggttgaa actactcttg
     4561 ctcctcttga aatttgactt gctagaggag aaagaactag agaaaatttt ggtcagtcta
     4621 gaaaagcaga ttgactttat gaaaagccac tatcgcgcca agtacaccct tagtaactgg
     4681 gggattttac aaacaattcc gatgcttgct atctattatt tcttttcaga taagatggac
     4741 ctagaagaag cttaccattt tgcttcagag gagttgaaac agcaaattga gacacagatt
     4801 ttaggagatg gaagccagtt tgaacagtcg attctctatc atgtagaggt ttataaagcc
     4861 ttgctggatt tgtgtctctt gcttccagac ttgcaagata gttaccgaga gttgctggaa
     4921 aagatggcga cctatattca aatgatgaca ggcttagatg gacgaacttt ggcttttggt
     4981 gatagcgatt ctacagaaac gacagaaatt ttgagcctgt ctgctgtggt tttgaacaag
     5041 gaagaccttc ttaacggtct ggatgttaaa gtcgatttgc ttagcctctt gttcctgggg
     5101 cgagaaaagg tcaagcgact gcaggaattt gaaaggagag cttggcagcc taagtccatg
     5161 atctttgaag actctggaca tgtctgcatt aaggatgaac atcgttatct atttttcaaa
     5221 aatggtccgc taggaagtgc ccatagccat agtgacgaga atagtttttg cttacagtat
     5281 caaggccaac ctattttcat agatgctggg cgttattctt atcgggagat atatgaacgt
     5341 tatctcttaa agagtgcttg gagtcattcg acctgcattg tagatgggaa agctccggaa
     5401 agaatcacgg gatcctggga atatgaatac tatcctcact ccctgttttg tcaccataaa
     5461 gaaagggagg gagtgcatta tattgagggg gcttattggt cagcagaacc tgatttgcct
     5521 tatcttcaca agagaaaaat cctcatgttg gtagaggatg tctggctctt ggtagatgac
     5581 atcaggtgtc aaggtcagca tgaggcgttg actcagttta tccttgacaa ggatgtgacc
     5641 tatcaagatg ggaaaatcaa tcagttgaga ctatggagtg aagttgattt tgatttggaa
     5701 gataccatca tttctcctaa atacaatgag cttgaaagaa gtagcaaact caccaagcgc
     5761 caattctttg agaatcagat gctggattat accatcattg cgcatgagag ttttgaaatt
     5821 atccgtcatt ctgtctacca gacagatgat cgtgaagtgg aaaatgctct ggcttttgaa
     5881 gtgaaaaatg acgaaacaga caagctgatt ctgttattaa gcgaggatat tggtgtaggt
     5941 gaaaaattgt gcctcgttga cggaacaaaa atgcgtggaa aatgtctagt atatgataaa
     6001 ataaatgaga gaatgattcg cttgcagtgc tagaaatagg cattttgaat agtgaatatg
     6061 ttataataag tattagtagg aggtgtttta gattggagaa gaaactgacc ataaaagaca
     6121 ttgcggaaat ggctcagacc tcgaaaacaa ccgtgtcatt ttacctaaac gggaaatatg
     6181 aaaaaatgtc ccaagagaca cgtgaaaaga ttgaaaaagt tattcatgaa acaaattaca
     6241 aaccgagcat tgttgcgcgt agcttaaact ccaaacgaac aaaattaatc ggtgttttga
     6301 tcggtgatat taccaacagt ttctcaaacc aaattgttaa gggaattgag gatatcgcca
     6361 gccagaatgg ctaccaggta atgataggaa atagtaatta cagccaagag agtgaggacc
     6421 ggtatattga aagcatgctt ctcttgggag tagacggctt tattattcag ccgacctcta
     6481 atttccgaaa atattctcgt atcatcgatg agaaaaagaa gaaaatggtc ttttttgata
     6541 gtcagctcta tgaacaccgg actagctggg ttaaaaccaa taactatgat gccgtttatg
     6601 acatgaccca gtcctgtatc gaaaaaggtt atgaacattt tctcttgatt acagcggata
     6661 cgagtcgttt gagtactcgg attgagcggg caagtggttt tgtggatgct ttaacagatg
     6721 ctaatatgcg tcacgccagt ctaaccattg aagataagca tacgaatttg gaacaaatta
     6781 aggaattttt acaaaaagaa atcgatcccg atgaaaaaac tctggtattt atccctaact
     6841 gttgggccct acctctagtc tttaccgtta tcaaagagtt gaattataac ttgccacaag
     6901 ttgggttgat tggttttgac aatacggagt ggacttgctt ttcttctcca agtgtttcga
     6961 cgctggttca gccctccttt gaggaaggac aacaggctac aaagattttg attgatcaga
     7021 ttgaaggccg caatcaagaa gaaaggcaac aagtcttgga ttgtagtgtg aattggaaag
     7081 agtcgacttt ctaaaatgaa ggaaaatgac ttgcaatttc tgttaagaaa taaaataatc
     7141 ccacctagaa caagctaggt gggattattt gcctatgaaa tgagaaatta tgggagcaag
     7201 ctcctaaatc aactgttttt gatctacttc tttaactact tgataaaagt tatagaagta
     7261 ggccaaactt gaaatgatgg ttacgactag gaatattgaa aatttccatt ggacagggtt
     7321 ggttaaaagt tgtggaaagg atatgaggag aaagaagagg gctgcgttga ggacaggtat
     7381 ccgttttgat tgtattttct caagtccttt attgagcgca ggaagaaaga ggagtaggag
     7441 tagtaaaact gtatgagaaa cagctcctga aataagggcg aagaaaagga aaatactgat
     7501 aaaaacatga atgatcagta gtctagctag tgatttcata aggcacctcc taatcctggt
     7561 cttttttagc tcttgcaata cgaagtgagt cgacaatatg tatcatcact ccgaaaaaga
     7621 aagctcccag tatagtttta aaaatatgtt ttgtatttag aagagaactg ataaaatttg
     7681 gattttcact tgttagggta tcaatgagtg gaattataaa aaatatcact gttccataaa
     7741 tcgaacctgc tttcagacca ggataacgta actgtttctt ttcttttttc atgagtttgc
     7801 tcctttttta aaaatcttcc ttccaaaaga gactgttgag gtcagtttgg aggctgcgag
     7861 cgagattgag acagagttcc agagttggat tgtacttgtc gttttcaatc atattgatgg
     7921 tctgtctcga gacaccgata tccttggcga gctcgagctg ggaaatgccc agttccttgc
     7981 gaaattcttt cacacgattc atctggtctc ctttctgatt tgtgtcgtat atatttgact
     8041 ttattatagt cttttaaaca taaagtgtca agtatttttg acatattttt tgaagaaata
     8101 gtagtctcct tgtcctattt gtctgacaag tgcaagctgg tcggatttgt ggtaaaatag
     8161 ataagatatg acaaaagaat ttcatcatgt aacggtctta ctccacgaaa cgattgatat
     8221 gcttgacgta aaacctgacg gtatctacgt tgatgcgact ttgggtggag caggccatag
     8281 cgagtattta ttaagtaaat taagtgaaaa aggccatctc tatgcctttg accaggatca
     8341 gaatgccatt gacaatgcgc aaaaacgctt ggcaccttac attgagaagg gaatggtgac
     8401 ctttatcaag gacaacttcc gtcatttaca ggcacgtttg cgcgaaactg gtgttcagga
     8461 aattgatgga atttgttatg acttgggagt gtctagtcct caattagacc agcgtgagcg
     8521 tggtttttct tataaaaagg atgcgccact ggacatgcgg atgaatcagg atgctagtct
     8581 gacagcctat gaagtggtga acaattatga ctatcatgac ttggttcgta ttttcttcaa
     8641 gtatggagag gacaaattct ctaaacagat tgcgcgtaag attgagcaag cgcgtgaagt
     8701 gaagccgatt gagacaacga ctgagttagc agagattatc aagttggtca aacctgccaa
     8761 ggaactcaag aagaagggtc atcctgctaa gcagattttc caggctattc gaattgaagt
     8821 caatgatgaa ctgggagcgg cagatgagtc catccagcag gctatggata tgttggctct
     8881 ggatggtaga atttcagtga ttacctttca ttccttagaa gaccgcttga ccaagcaatt
     8941 gttcaaggaa gcttcaacag ttgaagttcc aaaaggcttg cctttcatcc cagatgatct
     9001 caagcccaag atggaattgg tgtcccgtaa gccaatcttg ccaagtgcgg aagagttaga
     9061 agccaataac cgctcgcact cagccaagtt gcgcgtggtc agaaaaattc acaagtaaga
     9121 gggaaaaaga tggcagaaaa aatggaaaaa acaggtcaaa tactacagat gcaacttaaa
     9181 cggttttcgc gtgtggaaaa agctttttac ttttccattg ctgtaaccac tcttattgta
     9241 gccattagta ttatttttat gcagaccaag ctcttgcaag tgcagaatga tttgacaaaa
     9301 atcaatgcgc agatagagga aaagaagacc gaattggacg atgccaagca agaggtcaat
     9361 gaactattac gtgcagaacg tttgaaagaa attgccaatt cacacgattt gcaattaaac
     9421 aatgaaaata ttagaatagc ggagtaagat atgaagtgga caaaaagagt aatccgttat
     9481 gcgaccaaaa atcggaaatc gccggctgaa aacagacgca gagttggaaa aagtctgagt
     9541 ttattatctg tctttgtttt tgccattttt ttagtcaatt ttgcggtcat tattgggaca
     9601 ggcactcgct ttggaacaga tttagcgaag gaagctaaga aggttcatca aaccacccgt
     9661 acagttcctg ccaaacgtgg gactatttat gaccgaaatg gagtcccgat tgctgaggat
     9721 gcaacctcct ataatgtcta tgcggtcatt gatgagaact ataagtcagc aacgggtaag
     9781 attctttacg tagaaaaaac acaatttaac aaggttgcag aggtctttca taagtatctg
     9841 gacatggaag aatcctatgt aagagagcaa ctctcgcaac ctaatctcaa gcaagtttcc
     9901 tttggttcga agggcaatgg gattacctat gccaatatga tgtctatcaa aaaagaattg
     9961 gaaactgcag aggtcaaggg gattgatttt acaaccagtc ccaatcgtag ttatccaaac
    10021 ggacaatttg cttctagttt tatcggacta gctcagctcc atgaaaatga agatggcagc
    10081 aagagcttac tgggaacctc tggaatggag agttccttga acagtattct tgcagggaca
    10141 gacggtatta ttacctatga aaaagaccgt gtaggaaata tcgtaccagg tacagaactg
    10201 gtatcgcaac aaactgtgga tggcaaggat gtttatacaa cattgtctag tccgctacaa
    10261 tctttcatgg aaactcagat ggatgccttt ctagaaaaag taaaaggtaa gtatatgacc
    10321 gcgaccttgg tcagtgcaaa gaccggtgaa atcctcgcta ccacccaacg acctaccttt
    10381 aatgcagata ctaaagaagg aatcactgag gactttgttt ggcgtgatat tctttatcaa
    10441 agtaactatg aaccaggatc agccatgaag gttatgacgt tagcttcttc tattgataat
    10501 aataccttcc caagtggaga atacttcaat agcagtgaat tcaaaatagc ggatgcgacg
    10561 actcgagatt gggatgttaa tgatggtttg actactggtg ggatgatgac tttcttacaa
    10621 ggtttcgctc actccagtaa tgttggaatg agtctacttg aacaaaaaat gggagatgct
    10681 acttggttgg attatctaaa acgctttaaa tttggggttc caactcgctt tggcttgaca
    10741 gatgaatacg ctggtcaact tccagctgat aatattgtta gtattgctca aagctcattt
    10801 gggcaaggaa tttcagtgac acaaacacaa atgcttcgtg cctttacagc tattgctaat
    10861 gatggagtta tgctggagcc aaaatttata agtgctattt atgatactaa caatcagtct
    10921 gtacgtaagt cacaaaaaga aatagtagga aatcctgttt ccaaagaggc agcaagcaca
    10981 actcgaaatc acatgatctt agttgggacg gaccctctat atggaactat gtataatcac
    11041 tacacaggaa agccaattat aacagttcct ggacaaaatg tagcagttaa atccggtacg
    11101 gctcaaatcg ctgatgagaa aaatggagga tacttggttg gttctaccaa ttatattttc
    11161 tcagttgtga ctatgaatcc tgctgaaaat cctgatttta tcttgtatgt aacggttcaa
    11221 cagcctgagc attattcagg tatccagttg ggagaatttg ccaccccaat cttggagcgg
    11281 gcttcagcta tgaaagaatc tctcaatctt caatctccag ccaaaaattt agataaagtt
    11341 acgacagaat cttcttatgc aatgcctagc atcaaggata tttcacctgg tgagttggcg
    11401 gaagccttac gccgaaatat tgtgcaacca atcgttgtag gtactggaac aaagattaaa
    11461 gagacttctg tagaagaagg gaccaatctt gcaccaaacc aacaagttct ccttttatcg
    11521 gataaggtag aagaaattcc agacatgtat ggctggaaaa aagagactgc cgagaccttt
    11581 gctaaatggt tggatattga actggaattt gaaggttcag gttccgttgt tcagaagcaa
    11641 gatgttcgga ctaatacagc tatcaaaaac attaaaaaaa ttaaattaac tttaggagac
    11701 taatatgttt atttctatca gtgctggagt tgtgacattt ttaataactt tagtaggaat
    11761 tccggccttt atccaatttt atagaaaggc gcaaattaca ggccagcaga tgcatgagga
    11821 tgtcaaacag catcaggcaa aagctgggac gccaactatg gggggacttg ttttcctcat
    11881 tgctgcagtt gtggtgagtt tccttcttgc tcttttttca aaacaattga ccaataatgt
    11941 gggaatgatt ttgttcatct tggtcttgta tggcttggtc ggatttttag atgacttcct
    12001 taaggtcttt cgtaagatca atgaggggct taatcccaag caaaaattag ctcttcagct
    12061 tctaggtgga gttatcttct atcttttcta tgagcgtgga ggtgatatgc tttccgtctt
    12121 tagttaccaa gtgcatctag ggattttcta tattatcttc gctcttttct ggctagtcgg
    12181 tttttcaaat gcagtcaact tgacagacgg tattgacggt ctagctagta tttccgttgt
    12241 gattagcttg tctgcctatg gagttattgc ctatgtgcaa ggtcagatgg atattcttct
    12301 ggtgattcta gccatgattg gtggtttgct cggtttcttc gtctttaacc ataagcctgc
    12361 taaagtcttt atgggagatg tgggaagttt ggctctcggt ggaatgctgg ccgcgatttc
    12421 tatggctctc caccaagaat ggactctctt gattatcgga attgtgtatg tttttgaaac
    12481 aacttcggtc atgatgcaag tcagttattt caaactgaca ggtggtaaac gtattttccg
    12541 tatgacgcct gtccatcacc attttgagct tgggggattg tctggtaaag gaaatccttg
    12601 gagcgagtgg aaggttgact tcttcttttg gggagtggga cttctagcaa gtctcctgac
    12661 cctagcaatt ttgtatttaa tgtaagaatg gcatcctgat gtttcagggt gtttttgtat
    12721 ttaaatacac aatgaaaatc aaagaacaaa ctagaaagct aactttaggc tgctcaaaat
    12781 atagtat
//
LOCUS       DAVMEI010000046        12782 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVMEI010000046 DAVMEI010000000
VERSION     DAVMEI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.046"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(410..2137)
                     /gene="ezrA"
                     /locus_tag="VYQ24_001004"
     CDS             complement(410..2137)
                     /gene="ezrA"
                     /locus_tag="VYQ24_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1968159.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2219..4165)
                     /gene="gyrB"
                     /locus_tag="VYQ24_001005"
     CDS             complement(2219..4165)
                     /gene="gyrB"
                     /locus_tag="VYQ24_001005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1968160.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4180..4743)
                     /locus_tag="VYQ24_001006"
     CDS             complement(4180..4743)
                     /locus_tag="VYQ24_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1968161.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4828..5382)
                     /locus_tag="VYQ24_001007"
     CDS             complement(4828..5382)
                     /locus_tag="VYQ24_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1968162.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5393..6616)
                     /locus_tag="VYQ24_001008"
     CDS             complement(5393..6616)
                     /locus_tag="VYQ24_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1968163.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6653..9103)
                     /locus_tag="VYQ24_001009"
     CDS             complement(6653..9103)
                     /locus_tag="VYQ24_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1968164.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9303..10232)
                     /locus_tag="VYQ24_001010"
     CDS             complement(9303..10232)
                     /locus_tag="VYQ24_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1968165.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10372..10659
                     /locus_tag="VYQ24_001011"
     CDS             10372..10659
                     /locus_tag="VYQ24_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1968166.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10693..12027)
                     /gene="ciaH"
                     /locus_tag="VYQ24_001012"
     CDS             complement(10693..12027)
                     /gene="ciaH"
                     /locus_tag="VYQ24_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1968167.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12017..12691)
                     /gene="ciaR"
                     /locus_tag="VYQ24_001013"
     CDS             complement(12017..12691)
                     /gene="ciaR"
                     /locus_tag="VYQ24_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1968168.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3882 a         2732 c         2277 g         3891 t
ORIGIN      
        1 accgtggact aaagttgtac aagaaaagtg caaataagaa atctccagat taggaactat
       61 ccgtgagttc tctagtctgg agatttttca atagacttcg ttattggacg gttaccaagt
      121 tactcaaagt acagatttga agctgcagat aagactgacg aaatcacttt aatactaaat
      181 gaaaatcaaa aagcaaacta agaagctagc ctcaggctgt acttgagtac ggtaaggcga
      241 cgctgacgtg atttgaagag attttcgaag agtataaaac actgttttga ggtggtggat
      301 aaatcggctg ggtaaaaact caatctcagg agaaaatgaa gtaaatctcc ccacaactat
      361 agaaaaagat ttgattctgc tcctcacaca ataaaatctt tttcttttat taaaaacgaa
      421 tcgtttcacg tgttttctca tatgaggtaa caaagcgatt ggttacacca ggctctgcca
      481 cttccaatgc ttgagaaatc ttgtcaaatg aagcgtgata atcaaattct ttttcaaaaa
      541 tatctaaagc ttcgttaaat gcttcttgaa tgcgttcatc aaatgagcga tagcggttag
      601 aatattgcaa gagttgctct gtcaaagttg catattgtac aatattataa gtttccgttt
      661 ctaaagcttc catatcattc gttgcaattt caagaactcg ggtaacagat tcaatgttaa
      721 tcattttttg ttctaactca accattaaat cctcggtatt attgcttgcc gtaaagaata
      781 acttcaagaa agtttgtgga atacctggca gattgcgttt ttccatgtat cgcttgatag
      841 tatggagacg attgacataa acattggcct tttgacgtgc attaatatca tctttctcaa
      901 tttgtgtcag gcgctcacta actgaaattt gctcatcttc aatatctttt agttgagttt
      961 gtaaatcctc aagattttct tcaagaactg aataagcttg ggttggttct tcttgatttg
     1021 aagttacctc aacaatagct gcctcaaaac tctctaattc tgtctgaata cgacgaacat
     1081 ggcttgcagc tgtctcagga agtaaatagg tcttgttcaa acgtgcaata tcttctccca
     1141 ataaagtatt attctctttc atatgttgaa ggtaagttgg aagagttgca agtagatttt
     1201 ccactacttt ctgagcagca atttctcgag taaaaatatc atacaaggca ttgatttcct
     1261 cttgtgcctg tccattctca tattcggcat tatccaattc caactgacga atattctctt
     1321 ggtttttctt gaatgcttca taaagcaagt ggaaacgcgc ttcaatatcc gtttcaacaa
     1381 aatgataatt agcatcaatt agtttacgat aaccggattc caaatcctgt aattgatctg
     1441 gcaattctgt agaaagcgtc gtaaccaagg ctggaacacg atccacaata tgacttaagg
     1501 ccaaaatgtg attttctgta ttatccaaaa tcactgcggc ttccacaggg tcacccgatg
     1561 aattcaaggt tacaaattgt gaaaattcag attggatatt ttctaattgt ttttcaattt
     1621 catccaaggc ttgaccatac tgttctgaat tttcagcaac tctatgctga agttcctcaa
     1681 ataaatccaa agcatgaaga acacgaccac tatttttaga ttcttgcttc tctaagtctg
     1741 ccaaagcatt gcgaattgcc gcaatatctt cgtcaatcaa agtaatttga ctctcaattt
     1801 ggtcaatttg atgactggcc ttgagaaaac gaaatgaatg gttatagcct tctgcttcaa
     1861 agagattatt ttcaatatcg gcaaaagagt tgagagataa atcgacccat ttttgattcc
     1921 attcacggaa agccacttga ctttgtccaa tcaagtgcat attttttaca gcttctactt
     1981 catcatttac tggaagattg tatagttctt cttttctttc ttctagcgcc tctaatctcc
     2041 cctcgtttcg cttacgtaga aagattgcca ctacataagc cagaactaaa atgactgcaa
     2101 ttgcaaccat taaataaatt agttgtccat tagacatatc aaactccttt tttacttgaa
     2161 acaatcgtaa cgattatatc atatttttta gaccaaggga actacttctc cctatttttt
     2221 agacatcaag tgtactatag acagcatttt cttcgataaa ctcacgacga ggctctactc
     2281 gatcccccat caacatatca aagattttat ctgcttctgc agcatcatct acagaaactc
     2341 tagccatcaa gcgatgttcg ggatccatgg ttgtttccca cagctgatga tcgtccattt
     2401 cacctagccc cttataacgc tgaatagtcg gtttggtacg accttcacta taacgggcta
     2461 aagcttcttg gagtttgatt tcttgatctg cacccggctg gatatattct ttaatctcgc
     2521 ttccaacctt gacaccatag attggtggtt gggcaatata aacataacca gcttctagga
     2581 ttggtttcat ataacgataa atcaaggtta aaagaagggt acgaatgtgg gctccatcga
     2641 catcggcatc ggtcatcaaa acgagttttt ggtaacgggc tttcgaaaca tcaaattctg
     2701 cgccaaatcc tgttcccatg gctgtgaaaa gactacgaat ttcttcgtta gctagaatct
     2761 tatccatact tgctttttca acgttcaaaa tcttaccgcg aattggaagg atagcctgaa
     2821 actcacggtt acgaccagat ttggctgatc caccagctga gtctccttcg acgatgaaga
     2881 gttctgtttc agcagggtta ttagaagaac agtctgctag tttccctgga aggttggaaa
     2941 tttccaaacc agatttttta cgtgtgactt cacgcgcacg cttggcagcc acacgagcct
     3001 tggcagctaa aatccctttt tccacgatac gtttggcaat ctgtggattt tccatgagga
     3061 aatcggagaa ggcttcactg aagaggcgat tggtaatctt gaccacttcg ctatttccca
     3121 atttggtctt ggtttgtcct tcaaactgtg gatttgggtg tttaactgag ataactgcag
     3181 ttaatccttc gcgaacatct tcccctgtta aattatcttc attgtctttc agtaacttat
     3241 tcttacgagc ataatcattg ataacacgag tcagtgccgt acggaaacct tgttcatgcg
     3301 ttccaccttc atgggtatga atattattgg cgaaactcat gacattttca tggtaacctg
     3361 ttgtgtactg catggctacc tcaactgtga tatcatccat ctcaccgtct gtatagattg
     3421 gtgtatcaaa gattacatcc ttgttctcgt tgatatattc aacgtaacta gcaatcccac
     3481 cttcataatg ataatgcttg gtttgttcca aaccttggcg cttatctgta attgaaattt
     3541 gaagaccgcg atttagaaag gccaactctt gaatccgttt atttaattta tcaaaatcaa
     3601 agattgttgt ttcagtgaaa atttctgggt ccggtgtgaa gtgaaccgtt gttccagttc
     3661 tatctgtatc cccaaccacc tcaaggtctg caacaacatg accacgacgg tattcttggt
     3721 aatgaatctt accgttttta tggacatgaa cgtctaattg agtggaaagg gcattaacta
     3781 ctgacgaccc caccccgtga agaccacctg aaaccttgta tccaccaccg ccgaactttc
     3841 ctccagcatg aaggactgta aagacggtct caacagcagg acggcctgtt ttttcctgaa
     3901 tatcgactgg gataccacgc ccatcatcca caacagtaat cgaatcatct ggctcaataa
     3961 aaacttgaat atggctggca aatcctgcca aggcctcgtc aattgagtta tcaacaattt
     4021 cccagactag atggtgaaga ccttcttttg aggttgatcc aatgtacatc cctggacgca
     4081 tacgaacagc ctctaagccc tctaaaactt gaatttgact ggcatcataa tcctgtgcct
     4141 gcagattttt gatttcttct gtcatcttat tcctttttct tatatgtcta atacttgtct
     4201 taaattcacg atactggtaa acaagtgggt atcaagtcct gcagcttgac ctgcttcgat
     4261 atcaatcggc cgatcaccaa tgacaagacc agagctaatc tgatactttt ctcttaaata
     4321 aagcatggat tcgggatttg gctttctctt aaagcctgag ctagaagtca ccacttctgt
     4381 aaaataagct gctatagagg ttttttctaa aatttccaaa acctgatcat ttcgatgaga
     4441 gaccaaaaaa tgacggccac cttgatttaa aatgtcttcc aataggtcag aaactccttc
     4501 aaataaaatc gggtgttcaa gctctctggc ttcattttcc ttgtactttt ctaaaaaatt
     4561 ctctaaattg ggagcgaatg tctcaatcgc aaaaggagta gaaaccttta aagcttgata
     4621 gacactgtca tggtcttgtg tgataccata cagtgccaat gtttcaacaa atgcagctgt
     4681 tgaagtttca taattatcca gtaaagttcc acctaaatcc cagatgtaat cgtgatattt
     4741 catacccttc attatagcat ttttttatga aaaaagcgat gatttccctg agttttccac
     4801 ataaaaaacg agagaataac tcccgtttta ctgattttta ccaaagacat ctcgatagag
     4861 cattcctgtt cgtaaactct ctgcgtcccc tcctagttgc tccaccaact cgtaggcaaa
     4921 ggcaagggct gttgaaggac cccgactggt tgtcaactga ccatctacca ctactgtttc
     4981 cttgacgtag tgaccatcaa ggatttgctc ttgaacgccg tcataacaag tgtatcgctt
     5041 atttttcaat atctctgctt gattgagggc aattggtgcc gcacaaatgg ctgctagttt
     5101 cttcccttct tgctcgaagc tttgcaattc ttgaatcaag gtctgattat cacgtaaatg
     5161 tgcagaacca ggcatacctc caggaagaac aatcatatca tagtctgata aatctccatc
     5221 aaagacatga tctgctctta cttggattgc atgcgaaccc gttacttgct cttcaaaacc
     5281 aaccatatca catgtgatat tggctcgacg caagacatct acaactgtca aggcttcaat
     5341 ttcttcaaag ccctgagcta acataactgc tacttttacc atgattttct ccttatttaa
     5401 tttgttttaa tacaaccttt ttccgtttga atgggacttt tccgctcttt tcttcagcta
     5461 aattagtctg gtaactcatc gataaaagca aaccaacacc aatcaaatta ctgataatag
     5521 ctgatccccc ttgcgaaatg aaaggcaagg gaatccccgt caaaggaagt agtccagtca
     5581 cagcaccgat attctcaaag atgtggaaga gcaacatcat aatcaaacct gtggaaatat
     5641 aagtgtagaa ctggttattt gatttaagag taatcttcaa catacggtaa atcaacatga
     5701 gatagagggc aataaccagg acagagccaa taaagccaaa atcttctgca ataaccgtaa
     5761 aaatcatatc tgactctcga actgggataa gcagattcga agcattaaat ccctgaccaa
     5821 ataagccacc actcccaatg gcaatctgcc cttgagcctg ctggtaagtc gttgtttggg
     5881 caaactcaaa gggattgagc caagccaaaa tccgattaat ttggtaggtc ggcattccaa
     5941 tctggtgaag aaaagctcgt ccgtccttgc taataaagat agctaagaaa ccagcaactc
     6001 ctgttacagc agtcacaaat actgggataa taattttcca agaaacccct gataataaaa
     6061 cgattcctga gaaaatggct acaaaaacca aagccgtccc caagtcactt tgaagtgcta
     6121 aaagaactag gactggaatg gtaaagagaa tcatccagaa aattaacaaa aagtccagcg
     6181 gaaccgtgcg tctccattcc ttatgtttct ttgtaaattg gacaatgaca cgagccaaca
     6241 tgaggatata ggatatcttc ataaattctg acggttgaaa tagggtaatt ccatttattg
     6301 atacccagtt tttggcaccc gttgatgcaa ctaagcttgg attataaaat acaatcggca
     6361 agatcataag tcccaagcct aaaatatata gaaagggggt caccttccaa agaaattctg
     6421 tattaaagag catgaccaca aaaccaatca caagccccaa ggcaatccag gcgacctgct
     6481 gccctaaaat gggcagaata ttattgggat aatcatgact aacggctata tagatagcca
     6541 ccacaccgat gaccagtaga aaaaatactg gcaagagcaa actataatcg actctagagt
     6601 cgagagaacg tttcatatgt actaacctta tactttcata caatactatt tatcaaagtt
     6661 cattaaaaaa tctatcaata gcctcgtcaa cttcggatcg agagatggtt ttaacagtcg
     6721 cttcttctgc tagagatgct actatttgtt tgccgtatcg ttttccgacg attctcctat
     6781 ccaaaataag agttaaggaa cgttggtatt cacgtctcat acttcttccc aaagcctgtt
     6841 ttaaacgaat aatggccatt ggcaattgat aatcataaaa ggcatttttc ccttcttgat
     6901 tcagttcttg attaatcttt ttcgtcaagg gttcttgagg attttggaaa ggaagcctcg
     6961 gtacaacttg aatcacagaa ggatggcttg aaaaatcaac tccctcccag aaacttgctg
     7021 caccaagcaa gatttgttgt tcaccttttt caaagcgttt ctttagctga tgaacatccc
     7081 catttttata ctgggccaag tggctaactg taagtaaatc cgatactgct agaagcatgt
     7141 ctttagcggt aaagagaacc aaaatcggtt gctggaaagc ttgaatttcc actagtaaag
     7201 cagctacctc tctggcatag acttctaagg aggtttctgt taccagaggg aaatctttga
     7261 ccataaccac ttcttgttcc tgttttcccc gagattcaat cttgacaaat ttggcttcag
     7321 gatagcctaa aaggtctgcc aaagaaaccc tctgactaat ctcaagagta gccgatactc
     7381 ccaagacttg gcaactctca ggaagtaaag aggacagaag agtacggcct gattttgtag
     7441 aagaaatctg aattttcttt tgactcgttt cagttacttc aagccagtaa tcaccttcag
     7501 ctgtaaaata gcgaaccagc tcatcaaagc cttccacttc taattctgaa aaatactgat
     7561 ggagattgtc cagagaatct aagatatttt tcctagattt accagactga aattgttcta
     7621 tcaagtagag acactcaaag cgaatacttt ctagtatccg ttgttgaacc ctgttttctt
     7681 ctcctactaa agccttatca attaaatcga taatagattg tatatcgtag gtctcttgaa
     7741 gcagattttc tagagctaac aaaatctttt ggacttcatc aataatcagt aaacggtcac
     7801 tgacaaattc aggattatct tcaagtctgg tcacgagata ggcatgatta gtcactaaaa
     7861 gcttgcaagt ctctgccctt tcttgactac gtttccaaaa atcttccgtc acaaataagc
     7921 tctgggatga taaattccca tcatgacgaa ggtctgctag aaaatgttgg taacggtaga
     7981 gttgcccgat ttcatccaaa tctcctgtct ctgtctcagt aagccagacc aagacttgca
     8041 ttttaaagcg tctaaataag cgattttcat cattttcctg caaggaatga taaaaggcat
     8101 ccaacttcag ataattttgt ggtcccttta agctatgaat atctgtatgg aacacttcct
     8161 tgagacgttt accttcttct tccatgattt gattttgaag aatctttgtc ggaacactaa
     8221 gaacaatttg tcgctctttg gattgagata aagcgggtaa gagatagcca taggttttcc
     8281 caatccctgt cggcgcttga atcagagaga aaggttcatc tttcaatagc aagccaacct
     8341 ctttagcaaa actttcttgt tcctccctca cttcaaggtt caacagagaa atatttttag
     8401 aaaagtcttg agatagtttt cgtagctcca gagaagctgc cgttttctta aaatacagac
     8461 cttgaacttg gaccaagtct ggagaactca ggatagattg gttgcgataa gtttcctcaa
     8521 taaccaggta agactcatat aggagagcgt cagccatttc cagcaagcgt tccaagagac
     8581 ctttaggaag ctgggtcatc ttttcccgta aaaaaagaag taattctgct gtagcttggg
     8641 catctgaaag ggctgtgtgt gcgtgtttaa gaggaattcc taattctcga cacaaaatcg
     8701 gcaagctata tttttccagt tcagggaaaa agacctgggc caattcgacc gtatcaacac
     8761 gagggtttct tagctcatag ccttcaaaaa ataaattttc cgccaagaga ttagcatcaa
     8821 actgaacatt atgggctaca aaaatcccat cctccaccaa gtcaaagatt tttctggcaa
     8881 cttgcgaaaa atcaggtgct tgcgccagac gttggtctgt caatcctgtc agttctttga
     8941 tatgagcatc caagggttca tgtggattga catccgtcgt atagtgatcg acgatttctc
     9001 catcctcaat cacgacaatt cccacttgga taattttagc cttactacct gtgctagttg
     9061 cctctaaatc caccacaaca tagcgattac caatcgtttt cattataaaa aattatacca
     9121 aaaaaagggc catttggcac ctgcaaacat aggataattt tcaaactcaa gaacactact
     9181 tttcttagaa atcccatcga atagagcatt ctagcctgct gattttttta aaatagtgaa
     9241 gaaatacgaa aaattgagag aagaaatgtt ttcatcttcc ttctctcaac gaatcatgta
     9301 ttttatttaa ccatgtcagg atcgtagtca taaaatcctg tatcaaggaa acggttttta
     9361 gggtgaagtt tacgaacttc ttcatccagc aagaaggctt ggtcatggct aaagttgccc
     9421 tcttgaatca agctacgcgc ttggataaag agttttgcaa tctcaacaaa gttctgacca
     9481 ggagtgtaga gcccttctag tttccagcga gtaaagccat gctccaccaa ttctgttaat
     9541 ttgatcatca aatcaaggtc attgttggca aagatatggg tcccatgatt atcttcaaaa
     9601 atggaatagt ggctctctgg atcacttggc tcagccaaga agaggtcacg tttatgcgtc
     9661 ttttcatcat cgatatgtgt aaagttatag tagttttgca agagtggacg tttagaatga
     9721 tggatgacgc tagcaccgta aaccaaaact tcagcaggaa tttccaaaat ctctggcatt
     9781 ttgaaaagtt cagctgatgg aatttcacgc gccaaaacag cctcagatgc gccagccttt
     9841 tgtccccaga agttaatctg acgactgcta gttaccatgg ttgaagcatc gtagatggtc
     9901 ttaaatgaat aaccatcgcg gttaactacg taaaagacgc ctgcatcccc aatcgtaata
     9961 tagtctgtct tgatttcttc caagaagtct aagaaaggct tgatacggtc catcatatct
    10021 tggtgcatga gagcattgac cgcaacgatc aattccttac cagcatcatg aaccaactta
    10081 gcgatttcac gtaattggtc ataactaaag gtcgttggca gacgaaggcc aaaatctttc
    10141 tcaccgacat agatacggtc tacgccagct tcgagtagtt gttcaacttg ttcaatactt
    10201 tcagcagttg ctgtaatgac aatcttttcc ataagaaaaa ttataccaca tttctccaaa
    10261 atcagccatg ctttgaaaat gaaaagggac tttattcttt ttgtaacctt tctgtaataa
    10321 ttctctgccg aaaaaatgat aaaataggta tgtactgtta aggagagaat catgcccgta
    10381 agaaaattac aatcctatga ggtagactat caagaagaat taaaccagca gcttcctcat
    10441 taccaagctt atacacctga agcgcaatct gatgccaatc tcaaggaaat cctatttttt
    10501 attaatatcg ctgttttttg tatctgtatt gctatcttta gttttatctt tttagcatta
    10561 aaattatcaa ctgctcttgc ctttgccgca gcaatcggat tcagcttact tgttttaaaa
    10621 gttcaacggt ctattatcaa acgaaaacgt agaagataaa tccaaaatat cgccccaatt
    10681 ggagcgatat ttttattttt tctttttaga tggtgtctga atggcaatct tcacttcaaa
    10741 gattgttccc ttgggtttat tatctttgac agtaacagtt ccttttagag catctacaat
    10801 ttgcttggct agggataatc ctaaaccaaa accacctttt tgccgggttc tagctttgtc
    10861 tactcgataa aaacggtcaa aaattttctt tttatcttct gtcgaaatac cgattccatt
    10921 atcagaaaca agtaaataaa gattgcgatc ggtcgccgag ataagaaaat caatttcacc
    10981 atcctcctca gtatacttga cggcattatc gaaaagaatg gtcatcagtt gtttcagaag
    11041 aagctgatct gtgacaattg ttcgatggat acgattttca aaacggaaga cacgattatt
    11101 ttccgaagca atcatctcat agtttgtgaa agttgtatta aaaaagctag ttggaacttc
    11161 tgcaagctcc ggcttaatcc catcatctct ccgagctaag ttcagcaagc ttgtcgttaa
    11221 aaaacgcata tttcggactt cttccaaact cgatgcaatg ctttcgctca catccataat
    11281 ggtagcttct ggcttacgaa aaagggtctc taagcgattt tgcaaaactg cgagtggagt
    11341 tcgtaactca tgactggcat tttccacaaa agactgttgc ttctgcatac tctcaagcag
    11401 gggcctaaca ctgaccctag ctagatagag actggcaagt aaagacaaaa tccagaaact
    11461 agccatcacg accacaatca attgctcatg cttttgacta gcctgctcca actgactggt
    11521 attaatcaag acagcagcat acttgatatt ggttgaaacc gaactaatat tggtctccat
    11581 caaaatcaca cgatagattt cttcctgccc atagctatta aaaacctgaa tctggtagat
    11641 atgtcctagt tctttctttt ctaacttaat cttatccaag cccaaaaatc gatttccaga
    11701 aagaagttga gtaaagtctt tatcaaagag aatgacttcc gtattggaac tgacattagg
    11761 ttttatttct actttactag cgtccgccct agcattttct aaatctttaa tctcttctgt
    11821 tgccctattt atagccagct gaataactgc ttgaggattt tcactcagtc catgaagctt
    11881 atcgtccacc gaagtataaa gactcgaatg catgacttgt aaaataatca gagtcattgt
    11941 agaaaaaatc agggtgaaga caccgaagtt gcggataaaa taactaaagt catccgcata
    12001 ccatgttttt ttaagtttac tgaacatctt ttaaaagata cccaacacta cgcaaggttt
    12061 gcaaattctc tgcaaaagtg gttcccttta atttcttacg gacttttgaa acatagactt
    12121 cgacaaccga aatcgttgta tcactatcaa atccccatag acggtcaaaa atctgcgtct
    12181 taggcaaaat cacattttga ttttgaagga aataaactag taaatcgaac tctttcccca
    12241 gcaattcgac aggagtatct tcaactttaa cggtattggt tgataaatta accacgatat
    12301 tcccataagt caaggtgttt tcattaaact tccctgaacg tttgagaagg gcctgaattc
    12361 gcattttaag ttcttctagg tagaaaggtt tggtcagata atcatccgct cccagttcaa
    12421 atccatgtcc cttgtcatcc aaactttcct tggcagtcat aatcagaact ggtgtcgtaa
    12481 ttcccttttc acgcaattct tttaagactt ggaaaccatt tttttctggc aacatcaaat
    12541 ccagcaaaat caagtcatag acaccactct cagcttcgta gagaccttct tctccatcaa
    12601 atacctgcat aacatccgca aaatcgtcta aaaagtcaaa tactgaattt gacagaccta
    12661 ggtcatcctc aaccaataag atttttatca tgagaaactc ctccttatta aaactattat
    12721 accaaatttg ccttaaaaaa aactcaactc tctgcatttt acatgagata gctgagtttt
    12781 ct
//
LOCUS       DAVMEI010000047        12611 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVMEI010000047 DAVMEI010000000
VERSION     DAVMEI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.047"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            33..425
                     /locus_tag="VYQ24_001014"
     CDS             33..425
                     /locus_tag="VYQ24_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968169.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            447..1238
                     /locus_tag="VYQ24_001015"
     CDS             447..1238
                     /locus_tag="VYQ24_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968170.1"
                     /translation="MKIPLLTFARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            1231..1872
                     /locus_tag="VYQ24_001016"
     CDS             1231..1872
                     /locus_tag="VYQ24_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968171.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLLVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            1876..2508
                     /locus_tag="VYQ24_001017"
     CDS             1876..2508
                     /locus_tag="VYQ24_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1968172.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            2536..2823
                     /locus_tag="VYQ24_001018"
     CDS             2536..2823
                     /locus_tag="VYQ24_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968173.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            2969..5482
                     /gene="secA"
                     /locus_tag="VYQ24_001019"
     CDS             2969..5482
                     /gene="secA"
                     /locus_tag="VYQ24_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1968174.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRSLKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            5563..6594
                     /locus_tag="VYQ24_001020"
     CDS             5563..6594
                     /locus_tag="VYQ24_001020"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1968175.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            6596..7627
                     /locus_tag="VYQ24_001021"
     CDS             6596..7627
                     /locus_tag="VYQ24_001021"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1968176.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            7666..8034
                     /locus_tag="VYQ24_001022"
     CDS             7666..8034
                     /locus_tag="VYQ24_001022"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1968177.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            8024..9127
                     /locus_tag="VYQ24_001023"
     CDS             8024..9127
                     /locus_tag="VYQ24_001023"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1968178.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFIEASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            9146..11161
                     /gene="recG"
                     /locus_tag="VYQ24_001024"
     CDS             9146..11161
                     /gene="recG"
                     /locus_tag="VYQ24_001024"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1968179.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            11477..12457
                     /locus_tag="VYQ24_001025"
     CDS             11477..12457
                     /locus_tag="VYQ24_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1968180.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         3687 a         2361 c         2875 g         3688 t
ORIGIN      
        1 tcttgaataa aaaatgaaag gaaacagaaa aaatgaaaat taagaaatta ttgaaaatgg
       61 ttattcctgt tttaatgata agtgctgttg gaactacttt cgtagaagca aatcagatag
      121 gtgcttttag taattttgtt attactacca gttataagag aacaggttat ttgactaaag
      181 aaaatgaagg tgcggaatat atcatgaact taaatccttg tagaaattta catcccatga
      241 ctgttaagca tcgtatagta aattctaatg gagaagctcg tagtggagaa tcgttaacaa
      301 cctgtggtac tcgttcaact catggtaatt gggcaacggt tggctatgtt tatgcagcag
      361 atatggcaag acaaaattgg tgggatttat cagctgccat ttcaggtagt tggtcaccaa
      421 attaattcgt aaagtaggta taatttatga agattcctct cttaactttt gcaaggcata
      481 aatttgttta tgtcttgctt actttgcttt ttcttgcttt ggtttatcgt gatgttttga
      541 tgacttattt cttttttgat attcatgcgc ccgatctagc taaattcgat ggacaagcaa
      601 ttaaaaatga cttattaaaa tcagcattag attttcgtat tctccagttc aatctaggtt
      661 tttatcaatc atttattatt ccaatcatca ttgttttgct aggttttcaa tatattgagc
      721 tgaaaaataa agttttacga ttgagtattg gaagagaagt gagttatcaa gggttaaaaa
      781 gaaagttgac tttgcaagtt gcaagtatcc cttgtttgat atatttagtg actgtgctga
      841 taattgcaat tataacctat ttctttggga ctttttctcc tcttggatgg aattctctat
      901 tttctgatgg aagtggttta caaagactcc tagatggaga gataaaaagc tatttgttct
      961 ttacttgtgt cctactaatc ggtattttca tcaatgcaat ctatttttta caaatagttg
     1021 attatgtggg gaatgtgact cgttcggcaa tcacctattt gatgtttctt tggcttggtt
     1081 ctatgctgct ctatagtgcc ttgccttact atatggttcc tatgacgagt ttgatgcaag
     1141 ctagctatgg ggatgtaagt ttgatgaaac tctttactcc ttatatcctt tatattgtcc
     1201 cttatatggt gcttgaaaaa tatgaagata atgtttaaga attttaacaa tatttggcta
     1261 aagagaaaga tcgttttact acttcgtata gttctgatga tgattttgat aaactatcta
     1321 ttgtcaacag cggttcaaaa gcaggatgct gttatctttt tcaagagaga attgatttca
     1381 atcttttcct ataatgacta ttctgaagcg aatttagaaa tccccaaact cttgttaaac
     1441 ctttcgcttt tcatggtagg atggctctct gtcattttac ttgaaagtga tttggcagac
     1501 cattaccatc acttgattcg ctatcaatca agctcctttt tcgattatac aaggaaacga
     1561 ttggttgtca tttctaaatt ttttactcaa gatttacttg tctggttcct tggtttactt
     1621 ccgctaggaa ttcatttcaa aacagtcgca cttttctttt tacttgctca gttaatgatt
     1681 ttgtacttac tactgtctta tctgatagca ctgattagtg cgggcgctgg tttttccttt
     1741 tttctctatt ttttagcatt tgtgggacaa gaatggatga tggatcatat tgtaacagtg
     1801 tatttactac tcttaagctt attagttatg ttgattgtta gtcgcttgga agagaaattt
     1861 aagaaaggat aaacgatgag acttgaaatt ataaatggac agaaaattta tgggaaaaga
     1921 cctattttaa atcagttgaa tttggtgttt caatcaggaa aaatttatgg acttaaaggt
     1981 gataatggat ctggcaagac ggttctttta aagatacttg ctggttatat taagcttgac
     2041 aaaggaaaag ttcttcaaga cgggaaagtc tacgggataa aaaatcatta tattcaggat
     2101 gcaggaattt taattgaaaa agtcgagttt ttatctcatt tatccctgag agaaaatttg
     2161 gaactgttaa ggtatttttc atctaaagtt acggaaaaaa gaattgccta ttggattcaa
     2221 tactatgatt tacaggaatt tgaagacatt gaataccgtc atttatcctt aggaacaaag
     2281 caaaaaatgg ccttgattca agcctttatt tcctctcctt ctatactctt tctcgatgaa
     2341 cctatgaatg ctttggatga gaagagtgtg aggttaacca aacaggtcat tttatcttac
     2401 ctgaaaaaag aaaatggtct ggttatcctg acatcgcaca tatcggaaga tatttcagac
     2461 ctttgtacag atgtattagt tgtcgaaaat ggacatatac aaatgtaaag gatatacaat
     2521 cctaggagat ggcttatggc acatctaaaa tcatttatta cacgctattc caaggtttat
     2581 attggtttag ttctgctgat ctggctgtct ttcttcttta tcccttggga tagagcgatt
     2641 ctgggggtaa ggattgacat cttcatcata cagaaaatct tgctagcttt tggaattctg
     2701 tccattctca tggccttgct gtccaagaaa gtcagtctct ttgtttttgg actgatttgc
     2761 tgtctttctc tttggattaa cttatttatc acatttgcca ttttgccgat ttttggcaat
     2821 taaacagtca taaaagtcgg agaggttagc ttgaaaacta acctcttttt ccttttcaaa
     2881 atggggattc ttccttgaaa ataatcagta attgtgctaa aattaaagga acattctaaa
     2941 atattcggaa tttaaagtaa ggaaaaacat ggctaatatt ttaaaaacaa ttatcgaaaa
     3001 tgataaagga gaaatccgtc gtctggaaaa gatggctgac aaggttttca aatacgaaga
     3061 ccaaatggct gctttgactg acgaccaact aaaagcaaaa acagttgaat ttaaggaacg
     3121 ttatcaaaat ggagaatcac tggattcatt gctttacgaa gcatttgcgg ttgtccgtga
     3181 aggtgccaaa cgtgtcctag gtctcttccc ttataaggtt caggtcatgg gggggattgt
     3241 tcttcaccat ggtgacgtgc cagagatgcg tacaggggaa gggaaaacct tgactgcgac
     3301 catgccggta tacctcaatg ccctttcagg taaaggggtt cacgtagtta cggttaatga
     3361 atacctgtca gaacgtgacg cgactgagat gggtgaattg tactcttggc ttggtttgtc
     3421 agtagggatt aacttggcta ccaaatctcc aatggagaaa aaagaagcct atgagtgtga
     3481 tattacttac tcaactaact cagaaatcgg atttgactac cttcgtgaca acatggtcgt
     3541 tcgcgccgaa aacatggtac aacgtccgct taactatgcc ttggtcgatg aggttgactc
     3601 tatcttgatt gacgaggctc gtacaccttt gattgtatca ggtgccaatg cggttgaaac
     3661 cagtcagttg tatcacatgg cagaccacta tgtaaaatct ttgaacaaag atgactacat
     3721 catcgatgtg cagtctaaga ctattggttt gtctgattca gggattgaca gggctgaaag
     3781 ctacttcaaa cttgaaaacc tctatgacat cgaaaacgtg gctttgactc actttatcga
     3841 taacgccctt cgtgccaact acatcatgct tctcgatatt gactatgtgg tgagcgaaga
     3901 gcaagaaatc ttgattgtcg accaatttac aggtcgtacc atggaaggtc gtcgttattc
     3961 tgatggattg caccaagcta ttgaagccaa agaaggtgtg ccaatccagg atgaaaccaa
     4021 gacatctgcc tcaatcacgt accaaaacct cttccgtatg tacaagaaat tgtctggtat
     4081 gacgggtaca ggtaagactg aggaagaaga attccgtgaa atctacaaca ttcgtgttat
     4141 tccaatccca acaaaccgtc ctgttcaacg tattgaccac tcagaccttc tttatgcaag
     4201 tatcgaatct aagtttaaag cggttgtcga agacgttaag gctcgttacc aaaagggtca
     4261 acctgtcttg gttggtacag tagcggttga aactagtgac tacatttcta agaaattggt
     4321 tgcagctggt gttcctcacg aagtcttgaa tgccaaaaac cactatagag aagcccaaat
     4381 catcatgaat gctggtcaac gtggtgccgt taccatcgca accaacatgg cgggtcgtgg
     4441 taccgacatc aagcttggtg aaggtgttcg tgaacttgga ggactttgtg ttattggtac
     4501 agaacgtcat gaaagtcgtc gtatcgataa ccagcttcgt ggacgttcag gtcgtcaagg
     4561 agatccaggt gagtcacaat tctacctatc tcttgaagat gatttgatga aacgttttgg
     4621 ttctgaacgc ttgaagggaa tctttgaacg cttgaacatg tctgaagagg ccattgagtc
     4681 tcgcatgttg acgcgtcagg ttgaagcagc tcagaaacgt gtcgaaggaa ataactacga
     4741 tacccgtaaa caagtccttc aatacgatga tgtcatgcgt gaacaacgtg agattatcta
     4801 tgctcaacgt tacgatgtca tcactgcaga tcgtgacttg gcacctgaaa ttcagtctat
     4861 gatcaaacgc acgattgaac gtgtcgttga tggtcatgcg cgtgccaaac aagatgaaaa
     4921 actagaggca attttgaact ttgctaagta caacttgctt cctgaagatt ctattacgat
     4981 ggaagacttg tcaggcttgt ctgataaggc catcaaggaa gagcttttcc aacgttcctt
     5041 gaaggtttac gatagtcagg tttcaaaact acgcgatgaa gaagcagtta aagaattcca
     5101 aaaagttttg attctacgag tggtggataa caagtggaca gatcatatcg atgcccttga
     5161 tcaattgcgt aacgcggttg gacttcgtgg ctatgctcag aacaaccctg ttgttgagta
     5221 tcaggcagaa ggtttccgta tgtttaatga tatgattggt tcgattgagt ttgatgtgac
     5281 acgcttgatg atgaaagcac aaattcatga acaagaaaga ccacaggcag aacgtcatat
     5341 cagtacaaca gcgactcgca atatcgctgc tcaccaagca agtatgccag aagatttgga
     5401 tttgagccag attggacgca atgaactttg cccatgtggt tctggtaaga aatttaaaaa
     5461 ctgtcacggt aaaagacaat aaaatgagat agtttagagg cggatatctt gtgaaaagta
     5521 aatttttact gggtatccgt ttgctttata aggagatgag ttatggtatt tacagcaaaa
     5581 agctctaaaa taaatataga agaagttcgt gccttgtcaa aattagaagg tcaggctttg
     5641 gagaggaaat cacagagaga tcaagagcta gaagccatta tacgtggaga agaccagcga
     5701 attctcttgg taatcgggcc atgctcatct gataacgaag aagctgtcct tgaatacgct
     5761 aagcgtttgg cagtcctaca agaagaagtg gcagatcgta tctttatggt tatgcgtgtt
     5821 tatactgcca aaccccgtac caacggagat ggctataaag gcttgattca ccagcctaac
     5881 gcgacagaag cgcctagtct tatcaatgga atcaaagccg ttcgccatct tcactatcgt
     5941 gtcatcacag aaacagggat gacaactgct gatgaaatgc tttatcctga aaaccttccg
     6001 cttgtagatg atttgatttc ttacatggca gttggtgccc gttcagttga agaccagcaa
     6061 caccgctttg tggcaagtgg ggcaggattt tctactggtt ttaaaaatcc aacctctgga
     6121 aatctcaatg tcatgtttaa tgggatttat gctgctcaaa acaaacaaag tttccttttc
     6181 ttaggaaaag aagtagaaac aactgggaac ccgctttcac acgctattct tcgtggtgct
     6241 cttaatgagt atggaaaaaa tattcccaac tactattatg acaatttaat tgataccatt
     6301 gcccagtatg agaaaatggg cttggaaaat ccttttatca tcattgatac caatcatgac
     6361 aattctggta agcagtatat tgaacagatc cgaattgtcc gccagacctt gattaaccgt
     6421 gcttggaatg aaaaaattaa gcagttcgtt cgtggtttta tgattgagtc ttatctagaa
     6481 gatggtcgac aaaatgagcc agaagtattt ggtaagtcta tcacagaccc ttgcctgggt
     6541 tgggataaca cagaagctct tgtcagagaa atttacaaaa cgttaggaga ataagatggc
     6601 atttattgaa aaaggtcaag aaatcgatat ggaagtcatc aaggctgaaa cccaattgtc
     6661 tgcggaagcc ttgagactca aggaaagccg tgacagggaa ttggcagata ttatttcagg
     6721 ggaagatgac cgtattctct tggtgattgg tccttgctct tctgataatg aagaggcggt
     6781 cttggaatat gctcgccgtt tatccgcctt gcaaaagaag gtagcggaca agattttcat
     6841 ggtcatgcgc gtgtatactg ctaagcctcg taccaatgga gacggctata aaggattggt
     6901 tcaccagcca gatacttcta aggctccaag cctgattaac ggcttgcagg ctgtgcgcca
     6961 gttgcactac cgcgtgatta cagagactgg tttgacaacg gcagatgaga tgctttatcc
     7021 gtcaaatctg atcttggtgg atgacttggt cagctaccat gccgttggag ctcgttctgt
     7081 ggaagaccaa gagcaccgct ttgtggcttc tgggattgat gcaccagtag ggatgaaaaa
     7141 tccaacctca ggaaatttgg gtgttatgtt taacgccatc tatgctgctc aaaacaagca
     7201 aaccttcctt tatcatgggc aggaagttga gacatcaggt aatcctttgg cccatgttat
     7261 cctccgtgga gcagtcaacg agtatggcaa ttatatgccg aattactact atgaaaacct
     7321 actccaagcc attgaacgct atgaaaccat gggacttgaa aatcctttta tcctcattga
     7381 caccaaccat gataactcag gcaagcaata tatggagcag attcgaattg ttcgccagac
     7441 cttgcagaat cgtgattgga atgagaaaat taaaaagacg gttcgaggat ttatgattga
     7501 atcttaccta gcagatggtc gtcaaaacca accagagatc tttggttgct ctattactga
     7561 cccttgccta ggttgggaaa atacagaggc cttggtagaa gagatttatg ttaccttgac
     7621 aaaataagtg aaaaggatgg agttggggaa tctcaactcc ttttgatgag aatgatagtt
     7681 ggacacggaa ttgacatcga agaattggct tcgatagaaa gcgcagttac acgacatgaa
     7741 ggatttgcta agcgtgtact gaccgctcag gaaatggagc gcttcaccag tctcaaagga
     7801 cgcaggcaaa tagaatattt agctggtcgc tggtcggcta aggaggcctt ttccaaggct
     7861 atgggaacgg gcattagcaa gctcggtttt caggatttgg aagtcttgaa caatgaacgt
     7921 ggggcgcctt attttagtca ggcaccattt tcaggaaaga tttggctgtc tatcagccac
     7981 accgatcagt ttgtgacagc cagtgtcatt ttggaggaaa atcatgaaag ctagtccaca
     8041 tagaccaacc aaggctctga ttcatctggg agctattcga caaaatattc agcaaatggg
     8101 ggctcatatc cctcaaggaa cgctcaagtt ggctgtggtc aaggccaatg cttatggtca
     8161 tggagctgtt gccgttgcca aggcaattca agatgatgtt gatggctttt gcgtttccaa
     8221 tatcgatgaa gccattgaac tcagacaagc tggactcagc aagccaatcc tcattttagg
     8281 agtttctgaa atcgaagctg ttgctctagc taaagaatat gacttcacct tgacagtggc
     8341 tggactggag tggattcaag cactcttaga taaggaagtg gacctaactg gattgacagt
     8401 ccacctcaag attgattcag ggatgggacg gattggtttt atagaggcaa gtgaggttga
     8461 gcaggctcaa gatttgctcc aacaacacgg tgttcgtgtt gaaggaatct ttacccactt
     8521 tgctactgct gatgaggaat cagatgacta ttttaatgcc cagttagaac ggtttaaaac
     8581 tattttagct agtatgaagg aagttccaga gctggttcat gctagcaatt ctgcaacgac
     8641 tctttggcat gtagagacta ttttcaatgc ggttcgtatg ggagatgcca tgtatggtct
     8701 caatccaagt ggagcggtct tggatttgcc ttatgatttg ataccggcct tgaccttgga
     8761 gtctgctctg gttcatgtca agacagttcc agctggagct tgcatgggct atggagcaac
     8821 ttatcaagcg gatagcgagc aagtcatcgc gaccgtgcca atcgggtatg cagatggatg
     8881 gacaagagac atgcaaaatt tctctgtctt ggtagatggc caagcttgcc caattgttgg
     8941 cagggtttcg atggaccaaa tcactattcg attgcctaag ctttatccgc taggaaccaa
     9001 ggtaaccttg attggctcca atggggataa ggaaatcact gcaactcagg tagcgaccta
     9061 ccgcgtaacc attaactatg aggtggtttg cctcctcagc gaccgtattc cgagagaata
     9121 ttattagaaa agaaaggagt ggagcatgaa tctacatcaa cccttgcatg tcttgcctgg
     9181 tgtgggacca aagtcagcag aaaaatacgc caaactagga attgaaaact tgcaagatct
     9241 cttgctctac tttcctttcc gttatgaaga cttcaaaacc aagcaggtgc tggagctgga
     9301 agacggtgag aaggcagttc tttctggtca ggtagtgact cctgctagtg tccagtatta
     9361 tggtttcaag cgcaatcgcc tgcgttttag tctcaagcag ggagaggtcg tttttgcggt
     9421 gaatttcttt aaccagccct atctggctga taaaatagag ttgggagcaa cccttgctgt
     9481 ctttggaaaa tgggaccgcg ctaaggctag tctgactggg atgaaggttc tggctcaggt
     9541 agaagatgac ctccagcctg tttatcgtct ggctcaggga atcagtcagg ccagtctggt
     9601 taaggtcatc aagacggctt ttgatcaggg actggacctc ttgatagaag aaaatctgcc
     9661 ccagtcttta ctagacaaat acaaactcat gtcccgttgt caggcagtcc gagctatgca
     9721 ttttccaaag gatttggcag aatacaagca ggctcttcgc cgtataaagt ttgaggaact
     9781 cttttatttc caaatgcagc tgcagacgct caagtctgaa aatagaattc agggaagtgg
     9841 tctggttctg gattggtctc aggaaaaagt gacagcagtt aaagcaagtc ttccttttgc
     9901 cctgacccaa gctcaggaaa agagtttgca ggaaatttta actgatatga agtccgacca
     9961 ccacatgaat cgtctcctac aaggggatgt ggggagtgga aaaacggtag tcgctggctt
    10021 ggccatgttt gcggcagtga cagcaggtta tcaggctgcc ctaatggtac caacagaaat
    10081 cctcgcagag caacactttg agagtttaca gaaccttttt cccaatttga aactggctct
    10141 cttgacaggt tccttgaaag ctgcagaaaa gagagaagtc ttggagacca ttgccaaggg
    10201 tgaggctgat ttgattatag gaactcacgc tctgatacaa gatggggtgg agtatgctcg
    10261 tcttggtttg attattatcg atgagcagca ccgttttggt gtagggcaaa ggcgtatttt
    10321 acgggaaaaa ggtgacaatc cagatgtcct catgatgacg gcgactccca ttccacggac
    10381 gcttgccatc acagcctttg gagatatgga tgtttccatt atcgaccaga tgccagcagg
    10441 tcggaagcct attgtgacgc gctggatcaa acatgagcaa ctacctcagg tcttgacttg
    10501 gttagagggg gaaattcaaa aaggttccca agcctatgtc atctctcctt tgattgaaga
    10561 atcagaagct ctagatttga aaaatgccat tgccttatca gaagagctga cggctcattt
    10621 tgcaggcaag gcagaggtgg ctcttctaca tggtaggatg aagagtgacg aaaaagacca
    10681 gatcatgcag gatttcaagg agagaaagac ggatattctg gtttcgacga cggttattga
    10741 ggttggggtc aacgttccca atgcgactgt catgattatc atggatgccg atcgcttcgg
    10801 gctcagccag cttcaccagc ttagaggtcg tgtcggtcgg ggggacaagc agtcctacgc
    10861 tgttctcgtt gccaatccca agacggattc tgggaaagac cgcatgcgca tcatgacaga
    10921 aacgaccaat ggatttgtcc ttgcggagga agatttgaaa atgcgtggtt ctggtgagat
    10981 ttttggaacc agacagtcag gacttccaga gttccaagtg gctgatatta tcgaagattt
    11041 tccgatttta gaagaagcaa gaaaggttgc tagctacatt agttctatag aagcttggca
    11101 agaagatcca gagtggcgca tgattgccct tcatctggaa aagaaagaac atctggatta
    11161 agctttctct aaggaaaact taagctagtt tttaggtttg gctcttatac taaagtcatc
    11221 aaaaagaaac gaggactctc atatgacagt aacgattaaa gtaaattacc aaaccacttt
    11281 ccaaaagaag gaagcaaaaa actagtataa acagaagaga gagcgaaatg ctctttttcg
    11341 tttctaaaac tactttcagc ccatcatcct aaaagtaaag aatctaaatt cactttctat
    11401 ttatccttct ttcttgcatt gattacatag atatgctaca gttgtggtaa cgattacaaa
    11461 ataaaaggag catgctatga aaaatccagc tttgctagaa gaaattaaga cctatagagg
    11521 aagggatgag gttccggaag actttgatgc tttctgggat ggggaagtaa aaaatgtttc
    11581 cacgcttcca tcctaccact tggaggaaag agatttccac attcctcaag tcaagtgcta
    11641 tgagttaaca tttgaaggaa gcaaggaagg aaaggtctat gcacgcattg ttcttccaaa
    11701 gagtgaggag aaggttccac taatattcca ttttcatggt tatatgggac gtggctggga
    11761 ctgggccgac atgctgggct tcaccgtagc tggttacggt gttgtttcca tggatgtgcg
    11821 gggccagtca ggttactcac aagacggctt gcgttctcct ttaggaaata ccgtgaaggg
    11881 gcatattatc cgtggtgcta tggaaggtcg ggaccacctc ttttataagg atgtttatct
    11941 ggatatttac cagttggtcg aaattgttgc tagtctgtct caggttgatg agaagcgtct
    12001 ttctagctat ggtgcctcac aaggaggggc tctagctcta gttgcagcag cgctcaatcc
    12061 tcgaattcag aaaacagttg ccatttatcc cttcttgtca gacttcagac gggtgattga
    12121 gattggtaat actagcgagg cttacgacga acttttccgt tatttcaagt ttcacgaccc
    12181 cttccatgaa acagaggagg aaatcatggc gacccttgcc tatatcgatg tcaaaaatct
    12241 tgcccatcgt atccaaggtg aggttaagat gattacgggc ttggacgacg atgtttgcta
    12301 tcccattacc cagtttgcga tttataatcg tctgacctgc gataaaacct atcgcatcat
    12361 gcctgagtat gctcacgaag ccatgaatgt atttgtcaat gaccaagtct acaactggct
    12421 ctgtggaagt gagattcctt ttaaatatct aaaataagga gtcgactcta agcacaaaat
    12481 cttaaaaatt acaaacacgc atagtatcag gggattaaaa aaacttgata ctatgcgttt
    12541 tatcatggaa atatagtaaa atgaaacaag aataggacaa aacgatcagg acagtcaaat
    12601 cgatttctaa c
//
LOCUS       DAVMEI010000048        12505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVMEI010000048 DAVMEI010000000
VERSION     DAVMEI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.048"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..201
                     /locus_tag="VYQ24_001026"
     CDS             <1..201
                     /locus_tag="VYQ24_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968181.1"
                     /translation="LNANGAMATGWVKDGDTWYYLEASGAMKANQWFKVSDKWYYVNG
                     LGALAVNTTVDGYTVNENGEWV"
     gene            601..1722
                     /gene="mnmA"
                     /locus_tag="VYQ24_001027"
     CDS             601..1722
                     /gene="mnmA"
                     /locus_tag="VYQ24_001027"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1968182.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTTLLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTRDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1863..2318
                     /locus_tag="VYQ24_001028"
     CDS             1863..2318
                     /locus_tag="VYQ24_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1968183.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYTIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGFSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKFEDIQLVPAFLKTALPDWEGQLRHIHREE"
     gene            2328..4241
                     /gene="mnmG"
                     /locus_tag="VYQ24_001029"
     CDS             2328..4241
                     /gene="mnmG"
                     /locus_tag="VYQ24_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1968184.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDKILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDAVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4430..4519
                     /locus_tag="VYQ24_001030"
                     /pseudo
     CDS             4430..4519
                     /locus_tag="VYQ24_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4620..6299)
                     /locus_tag="VYQ24_001031"
     CDS             complement(4620..6299)
                     /locus_tag="VYQ24_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1968185.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQGEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6301..6534)
                     /locus_tag="VYQ24_001032"
     CDS             complement(6301..6534)
                     /locus_tag="VYQ24_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1968186.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6916..7137
                     /locus_tag="VYQ24_001033"
     CDS             6916..7137
                     /locus_tag="VYQ24_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968187.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7350..7502)
                     /gene="cibB"
                     /locus_tag="VYQ24_001034"
     CDS             complement(7350..7502)
                     /gene="cibB"
                     /locus_tag="VYQ24_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1968188.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7504..7689)
                     /gene="cibA"
                     /locus_tag="VYQ24_001035"
     CDS             complement(7504..7689)
                     /gene="cibA"
                     /locus_tag="VYQ24_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1968189.1"
                     /translation="MTNFDILDNQFLSLSENELSDIGGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7867..8550
                     /gene="tsaB"
                     /locus_tag="VYQ24_001036"
     CDS             7867..8550
                     /gene="tsaB"
                     /locus_tag="VYQ24_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1968190.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYVGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8547..8984
                     /gene="rimI"
                     /locus_tag="VYQ24_001037"
     CDS             8547..8984
                     /gene="rimI"
                     /locus_tag="VYQ24_001037"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1968191.1"
                     /translation="MIEIKRIQKHSDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8974..9984
                     /gene="tsaD"
                     /locus_tag="VYQ24_001038"
     CDS             8974..9984
                     /gene="tsaD"
                     /locus_tag="VYQ24_001038"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1968192.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(10024..11330)
                     /locus_tag="VYQ24_001039"
                     /pseudo
     CDS             complement(10024..11330)
                     /locus_tag="VYQ24_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11663..11957)
                     /locus_tag="VYQ24_001040"
                     /pseudo
     CDS             complement(11663..11957)
                     /locus_tag="VYQ24_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12064..12357)
                     /locus_tag="VYQ24_001041"
                     /pseudo
     CDS             complement(12064..12357)
                     /locus_tag="VYQ24_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         3554 a         2481 c         2928 g         3542 t
ORIGIN      
        1 ctaaacgcta acggcgctat ggcaacaggt tgggtgaaag atggagatac ctggtactat
       61 cttgaagcat caggtgctat gaaagcaaac caatggttca aagtatcaga taaatggtac
      121 tatgtcaatg gcttaggtgc ccttgcagtc aacacaactg tagacggata tactgtcaat
      181 gaaaacggtg aatgggttta agccgattaa attaaatcat gttaagaaca tttgacattt
      241 taattttgaa acaaagataa ggttcgattg aatagattta tgttcgtatt ctttaggtac
      301 ctgtcttatg atttcaggaa atgtcattaa aaaaacgact ccttttctct aacttggaaa
      361 atagattaga gaaaatgggt tgttttattt attatagtta tttgaatgaa gataagaaga
      421 aggtatactc acatcattca caaatctgta tattgactat aagttttaaa aaacaatttt
      481 taagctcttc cttgtcttct ctaaccaagc gtgttataat gaatactgct caagcgacct
      541 tcaatcgtga agcacacacg accttcaatc gtgaataaac gaatagatgg gagacttacc
      601 atgagtgata actctaaaac acgtgttgtc gtggggatga gtggtggtgt tgattcgtcg
      661 gtgacgactc ttcttctcaa ggagcagggc tacgatgtga tcggtatctt catgaagaac
      721 tgggatgaca cagatgaaaa cggcgtctgt acggcgaccg aagattacaa ggatgtggtt
      781 gcggtggcag accagattgg cattccctac tactctgtca attttgaaaa agagtactgg
      841 gaccgcgttt ttgagtattt cctagcggaa taccgtgcgg gacgcacgcc aaatccggac
      901 gttatgtgca acaaggaaat caagttcaag gcctttttgg actatgctat gaccttgggg
      961 gcagactatg tagcgactgg gcattatgct cgagtggtgc gtgatgagga tggcactgtt
     1021 cacatgcttc gtggcgtgga caatggcaag gaccagacct atttcctcag ccaactttcg
     1081 caagaacaac ttcaaaaaac catgttccca ctaggacatt tgaaaaagcc tgaagttcga
     1141 aaactagcag aagaagcagg tctttcgact gctaagaaga aagactcgac agggatttgc
     1201 tttatcggag aaaagaactt taaaaacttc ctcagcaact acctgccagc tcagcctggt
     1261 cgcatgatga ctgtggatgg tcgcgatatg ggcgagcatg caggtcttat gtactatacg
     1321 attggtcagc gtggcgggct tggtatcggt ggacaacacg gtggtgacaa cgcgccgtgg
     1381 ttcgttgtcg gaaaagacct gagcaagaat attctctatg taggccaagg tttctaccat
     1441 gattcgctca tgtcaactag cctagaggct agccaagtcc actttactcg tgatatgccc
     1501 gaggagttta cgctagaatg tacggctaaa ttccgttacc gtcagcctga ctctaaggtg
     1561 accgtccatg tcaaaggaga caaggcagaa gttatctttg cggaaccaca acgcgcgatt
     1621 acaccaggac aggcagttgt cttttacgat ggcgaagagt gtctaggtgg cggtttgatt
     1681 gacaatgctt accgcgatgg acaagtttgt cagtacattt agattgacaa attttctcaa
     1741 tttgctacaa taataacagc aatagaaatg atggtcaaag ctcatggatg ttgcaggctt
     1801 ttttgtcctg cacttctttg gagttttgac tgtttttgtg tcgtttaagg gaaaggacaa
     1861 gaatgactca acaagacttt cggacaaaag tagacaatac ggtttttggt gttcgggcga
     1921 cagctttgat tcttcaaaat cgcaagcttc tagtcaccaa agacaagggc aagtattaca
     1981 ctatcggcgg tgcgattcaa gttaacgaaa aaacggaaga cgcggtagtc cgtgaagtga
     2041 aggaagaact gggtgtcaaa gctcaagctg ggcagctagc ttttgtggtt gaaaatcgtt
     2101 ttgaacagga cggtttttcc tatcacaata tcgaatttca ttatctggtg gacttgctgg
     2161 aggatgcccc attgaccatg caggaagatg agaaaaggca gccctgtgag tggattgact
     2221 tggataagtt tgaggatatc cagctagttc cagccttttt aaaaacggcc ctaccagatt
     2281 gggaaggcca actaagacac attcatcgtg aggaatagga gagaaacatg acttatcatt
     2341 ttactgaaga atacgatatt attgtaattg gtgcgggaca cgctggggtt gaggcttcct
     2401 tggccgctag ccgtatgggc tgtaaggttc tacttgcgac catcaatatt gaaatgctgg
     2461 ctttcatgcc ttgtaatccc tctatcggtg gttctgctaa ggggattgtc gtacgtgaag
     2521 tcgatgccct cggtggcgag atggccaaga ccattgacaa gacttacatc cagatgaaga
     2581 tgctcaacac agggaagggt cctgctgttc gtgcccttcg tgcgcaggct gacaaggaac
     2641 tttactctaa ggagatgcgc aagacagttg aaaatcaaga aaatctgacc cttcgtcaaa
     2701 ccatgattga taagattttg gtggaagatg gcaaggttgt cggtgttcgt acagcgactc
     2761 atcaagagta tgctgctaag gctgttattg tgacgacagg gactgctctc cgtggggaaa
     2821 ttatcatcgg agacctcaag tactcatcag gtcctaacca cagcttggct tctattaacc
     2881 tagctgacaa tctcaaggaa ctgggtctcg aaatcggtcg tttcaagaca ggaacccctc
     2941 cacgtgtcaa ggcctcttct atcaactacg acgtgacaga aatccaacca ggagacgcag
     3001 tacctaatca tttctcatac acttcacgtg atgaggatta tgtcaaagat caagtgccat
     3061 gctggttgac ctataccaat ggtaccagtc atgagattat tcaaaacaac ctccaccgtg
     3121 cgcctatgtt tacaggtgtg gtcaagggag tggggcctcg ttactgtccg tcgattgaag
     3181 acaagattgt gcgctttgcg gacaaggaac gtcaccaact cttccttgag ccagaagggc
     3241 gcaatactga ggaagtctat gttcaaggac tttcaaccag tctgcctgag gatgtccagc
     3301 gtgacttggt tcattccatc aaaggtttgg aaaatgcaga gatgatgcgg acaggttatg
     3361 ctattgagta tgatatggtc ttgcctcatc agttgcgtgc gactttggaa accaagaaaa
     3421 tctcaggtct cttcactgct ggtcagacaa atggaacgtc aggttacgaa gaggcagcag
     3481 gccaagggat tatcgcaggt atcaatgcgg ctctaaaaat ccaaggcaag cctgaattga
     3541 ttctaaaacg aagtgacggt tatatcgggg tgatgatcga cgacttggtg accaagggaa
     3601 ccattgaacc ttaccgtctc ttgaccagtc gtgctgaata tcgtctcatt cttcgccatg
     3661 acaatgctga tatgcgcttg actgagatgg gccgtgagat tggccttgtg gacgatgaac
     3721 gctgggctcg ttttgaaatc aagaaaaatc aatttgataa tgagatgaaa cgcctagaca
     3781 gtatcaaact caagccagtc aaggaaacca atgctaaggt tgaggaaatg ggcttcaagc
     3841 cgttgacaga tgcggtgaca gccaaggaat tccttcgccg tccagaagtt tcttaccaag
     3901 atgtggtggc cttcatcgga ccagctgcag aggaattaga tgacaagatt atcgaattga
     3961 ttgaaacaga aatcaagtac gaaggctata tttccaaagc catggatcag gttgccaaga
     4021 tgaaacgtat ggaagaaaaa cgcattccag ccaatatcga ctgggatgac atcgattcta
     4081 ttgcgacgga agctcgtcag aagttcaaac tcatcaatcc agaaaccatc ggccaagcca
     4141 gccgtatttc gggagtaaac ccagcagata tttctatttt gatggtgtat ctggaaggta
     4201 aaaatcgtag tatttctaaa actcttcaaa aatcaaaatg atacgtcgta ggcttcttac
     4261 gaatgagttc aaagcttggc tttgattcat ctccaacctc ccatagttcc ccgaactatg
     4321 ggagctaact caagttatgc attttcctag ccttctggtc tgattttcat tgagttttaa
     4381 tctaaaaaga aagcatagag aaaaacagct ccgaagacgg ggctgttttg ttgatttgta
     4441 ctcaatgaaa atcaaagagc aaactaggaa gctagccgta ggttgctcaa agcactgctt
     4501 tgaggttgtg gatagaactg acgaagtcag ctcaaaacac cgttttgagg ttgtggatag
     4561 aactgacgaa gtcagtaaca tatatacggc aaggcgaagc tgacgtggtt tgaagagtat
     4621 tattcttcat ctggtgtgag gatcatcggg atgatgatcg gttcacgttc ggtattttca
     4681 tagaggaagg ggcgaatagc gttgacaatg gcaccattga cagattgcac gctagcatcc
     4741 ttatttttca gtgcgatacg aatggcattg aagaggatac gctggctttg acgaatcaag
     4801 tcgccagact ctctcatgta gacaaagcct cggctgagga tgtctggacc agatagaatc
     4861 atctgcgatt tgaagtcaac agttgcgact gccagaacga caccgtcttc agatagatcg
     4921 cgacgatctt tgaggacagc tgcgccaatt tcaccgatac gatttccatc gacatagata
     4981 tcttgggcgt tgaaatggcc tgcgatacga gctgagtcag cagtaagggc aagcacatcg
     5041 ccattgctca tgataaagat attgtccttc tcaacaccag tatccactgc tagtccagcg
     5101 tggacttttt gcatgcggta ttcaccgtgg acaggcatga agtattttgg cttaatcaag
     5161 cagagcatga gtttttgctc ttgctgacca ccgtgtccag atgtatggat attgttcact
     5221 ttaccgtgga taacttcgac accagcttca gaaatgatgt taatcagctt gttgacgcta
     5281 gtagtgtttc cagggatggg actagaagag aagataacgg tatcacctgg ttgtaattgt
     5341 acttgacggt gggttccgtt ggcgatacga gagagggctg ccataggctc accctgacta
     5401 cctgtacaga ggataagaac ttctcctgca ggataatctt tgatttcatt tggctcgata
     5461 aaggttccct taggagcttt gatgtagcca agatcgattc cgttgacaat ggccttttcc
     5521 atagaacgac caaagaccgc aatcttgcgt ccagtcttaa cagcagcttc tgttgcctgc
     5581 tggagacgga agatatttga ggcaaaggat gcaaagatga tacgtccttc aataccttgg
     5641 ataatcttca taatggactg accaacgact ttttcagagt tggtaaaggt tggtacttcc
     5701 gcatttgtcg agtcagacag gagacagagc acgccttctt caccaagcgc agccatacga
     5761 tgcaagtccg caggttctcc aactggagta aagtcgaact taaagtcacc cgtacagacg
     5821 attttccctt gaggagtatg aatgacaatc cccaaaggct ctggaataga gtgagtcgtt
     5881 ctaaagaaag ttgccttgag atttttaaag gtcaactcgg tgttgtggtt gatttcgtaa
     5941 agtttggcgt tgcgcaagag gccgtgttct tcgagtttcc cacggatcaa agccaaggca
     6001 agcggtccag cataaatagg gacatttgct tgcttgagta ggaacggaat cccaccaatg
     6061 tggtcctcgt gtccgtgtgt gattaaaaca gccttgacgc ggtcgatatt gtctacgatg
     6121 taagagtagt caggaatgac atagtcgata ccaagcaagt catcttctgg gaatttaatc
     6181 ccagcatcga cgatgataat ctcgccttgg tattcaattc cgtaagtgtt tttcccgatt
     6241 tctcctagac caccgatggc aaaaacgccg acttcttcag gtttaagagt gtaggccata
     6301 ttagaactcc gtaatttcga aggcgccagt ttctttttcg taatcgagca atttgtcaga
     6361 caagagttcg atatattcga tattgtactc tgggcgattt tcttcgacaa gttggcgagc
     6421 agtgatacgg ccctcaagtt ctgagctggc atcgatgtct aggtaaagcg cgcgtgttgt
     6481 ttcacggcgt gggctacgtt cttttgtttc ttgataaaaa actttgtaaa tcatatagtt
     6541 cctttctatt tacaggtcag cttattccaa acttttagca gatatttgct tgatttcatt
     6601 ccattttgtg tctagattga ccttgattcg ttacaattat ttcaattata ccacaaaatg
     6661 aaaaattagt aaaagacgga aacgagaaag ttaggggacg atttcttcgg taagcgtttg
     6721 cgaaaataaa gaaaatgtat tatgttgata acggaagtga aatttattgc ttgtcataat
     6781 tccatataga ttttaagtca aggaggttgc cctatgtata acttatttgc ttttgctatc
     6841 ggagttcgtt tgctagcttt tatcgttttt gttttgcgtt tatggaggaa atcttctatt
     6901 tgccaaactg gaacgatgac aaaccaagtt cttgaggaaa attacctttg tgactctcta
     6961 ctcgcttttt ctcctcctcg tgacctatgt cgtttggttt gtattttact ttggattaaa
     7021 tgcttagatg ccctgcttat gctggggttt tttgtttggg gatgtagaga tagtagattt
     7081 acgacacgaa aattccaccc catctatcaa gatgaggtgg tctttgcatt tttctaaaat
     7141 gatatgacaa tggaattttc aaagcttatt atttttatgg gagtcattgt tctgctcaaa
     7201 aatagagttg aagcataaaa ataaaacaat acaaacagat aaatcgaatg gtaaagggat
     7261 ggtagtaatg ttcaaatcat tgagaatgct gagcaaactg taagagatga agacaataaa
     7321 atttatcctt ctaaaaatgt gtttcttatc tagcatgact taccaaactt tttacgaagg
     7381 tcttttccaa tggtataacc tgccataaat aaagcgatac caacacctac accaaagcca
     7441 cccttgattt cttgcaattc cttattagaa atttcacgac agttgttcaa atctttcatc
     7501 atattaatct cctcctaaaa aataaccagc tgccaaacca gaacctataa gtgctgttcc
     7561 acctgcaata gctttccaag atactgctac tccaaagata accaagggag cgagaccgcc
     7621 accaatatct gataattcat tttcagataa ggataaaaat tgattgtcaa gaatgtcaaa
     7681 atttgtcatt gaactttcct ccttttttct tgttcacttt tatattcgga aaagaaaagg
     7741 aaaagtgagc atttttttaa attattttta gttaagtgag ggcactcttg tctgggggca
     7801 aagaaaattc ccatgtgcca gcttttgtag tataatagta agcagacaaa aagataggaa
     7861 tgtgttatga aagtattagc ttttgatacg tccagcaagg ctctttctct cgctatttta
     7921 gaggacaagc aggttcttgc cgagacgacg attaatatta agaaaaatca cagtattact
     7981 cttatgcctg ccatagattt tttgatggca agtttggatt ggacacccaa ggatttggac
     8041 cgaatcgtgg tagctgaagg gccgggtagc tacacaggct tgcgaattgc ggtagccact
     8101 gccaagactt tggctcatac tctgaacatc gagttagttg gtatgtctag cctcttggct
     8161 ctggtaccct accaacaaga aggcttgttc gtctctttga tggatgcgcg tcgcaacaat
     8221 gtttatgtag gattttatga aaatgctaaa cccgtcatgc cagaagctca cttacccttt
     8281 gagcgagtga ttgagttaat caagggtgct agtcaggtta cctttgtcgg agaagttggc
     8341 ccctttgttg agcagattca agaatacttg ccaaggacta atttcaaaga aaccttgcca
     8401 aatgcagcta atctagctct atgggcctgg gacaaggaag cagactcctt gcatgatttt
     8461 gtgccgaact acctcaagcg tgtcgaagct gaggaaaact ggctcaagaa tcacaccgag
     8521 tctggcgagt cttacattaa acgcttatga tagaaatcaa acgaatccaa aaacattctg
     8581 acttggctca agccatctac gctgttatga tagctgttta cccagtcagt ccttggacgc
     8641 tggaacaaat ccaagcagac ctgtcccaag accaaacttg gtatgctttg gcttatgatg
     8701 gggcagaagt gattggattt ctagctgtgc aggagaatct ttttgaagca gaagtcctgc
     8761 aaatcgctgt caaaggagct tatcagggtc agggaattgc gtcagccttg tttgctcaat
     8821 tgccgacaga caaggaaatt ttcctcgaag tcagacagtc aaatcaacga gcgcaagcat
     8881 tttacaagaa agaaaagatg gcagttatcg ctgagcgaaa ggcctactac catgacccag
     8941 tcgaggacgc cattatcatg aagagagaaa tagatgaagg atagatatat tttagcattt
     9001 gagacatcct gtgatgagac cagtgtcgcc gtcttgaaaa acgacgatga gctcttgtcc
     9061 aatgtcattg ctagtcaaat tgagagtcac aaacgttttg gtggcgtagt gcccgaagta
     9121 gccagtcgtc accatgtcga ggtcattaca gcctgtatcg aggaggcatt ggcagaagca
     9181 gggattaccg aagaggacgt gacagctgtt gcggttacct acggaccagg cttggtcgga
     9241 gccttgctag ttggtttgtc agctgccaag gcctttgctt gggctcacgg acttccactg
     9301 attcctgtta atcacatggc tgggcacctc atggcagctc agagtgtgga gcctttggag
     9361 tttcccttgc tagccctttt agtcagtggt gggcacacag agttggtcta tgtttctgag
     9421 gctggcgatt acaagattgt tggggagaca cgagacgatg cagttgggga ggcttatgac
     9481 aaggtcggtc gtgtcatggg cttgacctat cctgcaggtc gtgagattga cgagctggct
     9541 catcaggggc aggatattta tgatttcccc cgtgccatga ttaaggaaga taatctggag
     9601 ttctccttct caggtttgaa atctgccttt atcaatcttc atcacaatgc cgagcaaaag
     9661 ggagaaagcc tgtctacaga agatttgtgt gcttccttcc aagcagcagt tatggacatt
     9721 ctcatggcaa aaaccaagaa ggctttggag aaatatcctg ttaaaaccct agttgtggca
     9781 ggtggtgtgg cagccaataa aggtctcaga gaacgcctag cagccgaaat cacagatgtc
     9841 aaggttatca tcccccctct gcgactctgc ggagacaatg caggtatgat tgcctatgcc
     9901 agcgtcagcg agtggaacaa agaaaacttc gcaggctggg acctcaatgc caaaccaagt
     9961 cttgcctttg ataccatgga ataaagagtg gctctttgtc aagtgtagtg ggtaggcgaa
    10021 aagctacaat ctggagagta cgaaattcgt actctctttt ttgatgttca aagtcatcaa
    10081 aatccgtttt ttaaaattgt taaagttccg aaaaccaaag gctttgcgct tgataacctt
    10141 aataaaatta ttggtcgctt caagtttagc gttggaataa ggcagcttaa gtgcgttgat
    10201 aatcttatct ctatccctta aaaaagtcca aaagaccgtt tgaaaaagcg gatgaaccgt
    10261 tggcagttct tgctcaatca atccaaagaa atggttggca ttcttctctt gaaaatgaaa
    10321 gagcaggagt tgatagagtt cgtagtgaac ctgtaatccc tcagagtaag aaagcagctt
    10381 atctcgtact tcccgatggg ttaagtgcat tcgaaacgtg ggctgataga atcgtttctg
    10441 acagagcttg aagctatcct ggtgtagtaa atcagtccag tggactgatt tagcccgagc
    10501 ctagaaacga aaaagcgagg gttccaaaag cgcttgagcg cccgatactc caaggatttt
    10561 cggtcaaatt ggttcatgat ttggattcgt actcggttca tagctcggct cagatgttgg
    10621 acaatgtgga ggcggtcaag aacaatcttc gcctttggaa agagttgctt agcgagccga
    10681 taataagggc tgtacatgtc catggtgatg acctcgacca gctcccgaac ctctctctga
    10741 tagcgttgga aatggtttcg aatcaccgca tgagttcgcc cgtctaaaat tgcgaggatg
    10801 gatttggact caaaatcttg ggcaatgaag ctcattttgc tctttttgaa gctatactca
    10861 tcccaactca taacttttgg caacttggtc caatcggttt caaacttaaa ttccctcagt
    10921 tttcggatga cggtggaagt tgagaccgcc aatctgtggg caatctcagt catagactgc
    10981 ttttcaagga ggagctgagc gattttttgt ctcaccatgt tggaaatctg gcaatttttc
    11041 ttgacaatgg atgtttgaga aacgaccacc ttaaggcaat tcttgcactg aaagcggcgc
    11101 tttttgagat gcagtaccgt gggtaaaacc tgacagtcga gaagcggaat tttagaggct
    11161 ttctggaagt cgtatttgat catcttccct tgacaatgag gacaaggagg agcgggggaa
    11221 tccaactttg cctgaacgac tagatgggtt tggtatttca gaacagacaa gattttgata
    11281 tttttgtctt ccaatccgag caaatctgtg gtattcttaa gttgttccat atgaaccctt
    11341 tctaatgagt tggttagacg cttttcatta taggtcatat ggggcttttt ttctacaaga
    11401 aacgacccta taattcctgg ggtgggatta cccactacag aaattataga gccaaagcat
    11461 tccaaagtct tgtctgactc tggaatgttt tctgttcaca gttgttttgt aactgcctaa
    11521 taagtaaccg cgctataacg aggtatctac cgagattgtc aattctccag tatactgtta
    11581 taaaaaacaa aactggagga tttttttatg tccccccatc gtcccatcga ctgttccgta
    11641 gctgcccaat aacggagtat aatcagctga aacaagaata ggacaaaagt gcctcaagaa
    11701 aagcatcgta atttgacaat attattctga gatgtttttg atgtaagccc atattttctc
    11761 aatgggatta tactcgggtg aatagatagg aagtggtaac agtctatgcc ctgctcctta
    11821 cacatgttca ttctgtgaaa ccttgcattg tccataatga taagggatgg tgtatctaaa
    11881 gtaggtagta agaatttttg gaagcaagct tcgaaaaagt cactcgtcat cgtatcttta
    11941 taggtcatcg gagccatagt atattgaaac aaaaaagggc attagtactt ctataacagt
    12001 atcgcaatct ctggtgtgcg ttgtctcgat cttaattcaa ataattatat cttcttccag
    12061 agagactaac tgcctgctca aggctcgatc atattcgaga taaaaatagg tcttaacccc
    12121 tgtctcataa atataaatag gagtcaggta gcttaagtga ttcaattctt taaggaaccg
    12181 atttactttt tctgggtctt gttcgcagta ggtacggctc ttttttagac tatatcccat
    12241 agcctttaga gcgtaatgaa tagttgttgg aggacagttg aattcagcag ctatttcagt
    12301 caaataagca tcgggatgag tttcaatata gatctttaat ttatctctat caactttttc
    12361 aattaatagt ggaagttttc ttaaagttag tgtattgaaa cgtttttata cagtgctaaa
    12421 atttgaagtg tagtgcagat atatttgtaa tagtattata gaaattcttt gaagaaggtc
    12481 gagacaagag aaaataggta tcttt
//
LOCUS       DAVMEI010000049        12433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVMEI010000049 DAVMEI010000000
VERSION     DAVMEI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.049"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..503)
                     /locus_tag="VYQ24_001042"
                     /pseudo
     CDS             complement(1..503)
                     /locus_tag="VYQ24_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(565..1014)
                     /locus_tag="VYQ24_001043"
     CDS             complement(565..1014)
                     /locus_tag="VYQ24_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1968193.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(1016..1774)
                     /locus_tag="VYQ24_001044"
     CDS             complement(1016..1774)
                     /locus_tag="VYQ24_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1968194.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAKPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1889..3868)
                     /gene="pknB"
                     /locus_tag="VYQ24_001045"
     CDS             complement(1889..3868)
                     /gene="pknB"
                     /locus_tag="VYQ24_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1968195.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3865..4605)
                     /locus_tag="VYQ24_001046"
     CDS             complement(3865..4605)
                     /locus_tag="VYQ24_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1968196.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(4620..5933)
                     /gene="rsmB"
                     /locus_tag="VYQ24_001047"
     CDS             complement(4620..5933)
                     /gene="rsmB"
                     /locus_tag="VYQ24_001047"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1968197.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNALLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5926..6861)
                     /locus_tag="VYQ24_001048"
     CDS             complement(5926..6861)
                     /locus_tag="VYQ24_001048"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1968198.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHAFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6874..9270)
                     /locus_tag="VYQ24_001049"
     CDS             complement(6874..9270)
                     /locus_tag="VYQ24_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1968199.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKTQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9336..9650)
                     /locus_tag="VYQ24_001050"
     CDS             complement(9336..9650)
                     /locus_tag="VYQ24_001050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1968200.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9675..10301)
                     /gene="gmk"
                     /locus_tag="VYQ24_001051"
     CDS             complement(9675..10301)
                     /gene="gmk"
                     /locus_tag="VYQ24_001051"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1968201.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10431..12044)
                     /locus_tag="VYQ24_001052"
     CDS             complement(10431..12044)
                     /locus_tag="VYQ24_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1968202.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
BASE COUNT         3307 a         2960 c         2456 g         3710 t
ORIGIN      
        1 ttctcaacct tgcttctctc cttagatagc gcatggttac aggctttatc ttcagctgtt
       61 agcggcttga gtttgctgga tttacgtgga gtttgtgctt gaggatatat cttcatgagc
      121 ccttgataat cactgtcagc caagatttta ccagcttgtc cgatatttct gcaactcatt
      181 ttgaacaact tcatatcatg actatagttc acagcgatat ccaaagaaac aattctccct
      241 tgacttgtga caatcgcttg agccttcata gcgtgaaatt tctttttacc agaataattc
      301 gctaattgtt ttttagggcg attgattttt acttccgtca catcaatcat tatcgtgtcc
      361 tcaaagctga gaggagttct tgaaatcgta acaccacttt gaacaagagt tacttcaacc
      421 cattggctcc gacggattaa gttgctttcg tgaataccaa aatcagccgc aatttcttca
      481 taagtgcggt attctcgcac atatagaaag cgttatcaat ttatttatct catttttcag
      541 aaaattcttt tatttctgta aagtctacga tactcgatgt gtttttatat aatgatagag
      601 tctgagaatc actgttccgc tagccattcc aatagagatt accaaagcca acatgacaac
      661 caaggtcgca cttgccagtg ctttattata gtcccctgtc acaaaaaagg cagttgttcg
      721 gtaggagaga taacctggaa ccagcggtgc caaaatggcc aagataaaga ccacagcagg
      781 tgtcttataa agaatactta aaatctggct gacacaagaa ccaataatgg ctgcaatgaa
      841 ggtagctaca atgacattgg tcggttcctt gagcaagaga tagattagcc agacagtcat
      901 gcccaaaatc cctccaggta agagcataga ccgttgcaca ttgagtacga ttaaaaaagt
      961 gataatggca agaaaacttg ctactgcttg taataaaaag gttgttagtg tcatattagt
     1021 tcatcaatac caaggcgaca gaagttcctg cccctaaagc gagggtaatg agcagggatt
     1081 caaacatctt actcatacca gagtttatgt ggttggtcat aatatcacgg accgcattgg
     1141 tcaaggcaat acctggtaca aacggcatga ccgcaccagc tataatcaaa tctgccgttg
     1201 aaggaaaacc tgtgtagcga gcccaaaact gggcaattat cccaaagaca aaggctccag
     1261 caaaggctgt cacaaaggga attcggataa atttttccac atagagggaa aaggcaaaac
     1321 caaataaggt cgccactcct gccccaagtg cgtcgtagat atttccgcta aacataactg
     1381 aaaagaaagg agcactaaag gtcgcagcca gagttacctg caacttagta tagggaaggg
     1441 gtttggcttg caaggccgtc aattgcttaa aggctgtttc taagtcaatc tgccccccaa
     1501 ctagctgacg agaaatctgg ttcacatcgc agactttttc gatgttataa gaagaggagg
     1561 tcacgcgctt catgcgcgaa atattggtat tttcaataga gaaaaagata gcggcaggca
     1621 tggcaaggac attgcaatcc acaatcccct gcgaatgcgc gattcgaatc atggtatctt
     1681 ctacacgatg gatttctgag ccacttttaa ggagaatagt ccccgctagc ataatcacat
     1741 caatgacggc atttaattct tttgattctt ccatgctttc ctccttttat caactccctc
     1801 tattctatca caaatccgga ctcaaaaaaa atctttgcca tgaaatcatg acaaagattg
     1861 attactcatt ttgattatcc atctgctttt aaggagtagc tgaagttgtt ttaggtttgt
     1921 agattgaaat cttgactcta gtcttattta ggtctacctt ttcacctgct ctaggacttt
     1981 gttcaacaac catgccttct acactacctg caggcgctgt cgtcacttct acaacttcta
     2041 tattagcttc cttaatccca acaatttgaa tcaaattgtt cttagtaaac tccaagctgg
     2101 aaccaatgta actcggcatg gcaacacttg taactttttt agctactgtc aagacaattt
     2161 gagtaggttt actcacatca taagtcgttc cggcacctgg actttgtttc ataatcgttc
     2221 ctggttcgct ttcgctggac tcttcttcct ctatcttaat caaattctca ggaaccttct
     2281 tctgcttgag ttctgagatt acttctgtag agttccgtcc aatatagttc cctaattgaa
     2341 tcgtcgtagc ttttttagct actgtcaaaa caatttgagt tgccttgctc aagtcatagg
     2401 tcgtaccttc tggtagactt tgcttcagga ccgttccagc ctcactctca ttggactctt
     2461 cttcctcaat tttaatcaaa ttatctggaa cttttttctc ttttaattcc gcgataacat
     2521 cagaagattt ccggccgaca taattactaa tttggaagga ttgtttgcct gatgagacaa
     2581 ccagattgat tttcgttcct tcttttcgac cagttccagc gccaggatct gtacggataa
     2641 tccgcccttc ttccaccttt tcactagcct ctgtcttctc ctcaccaatc tcaaaattgg
     2701 cttttttgag cgttgccttg gcctctgcaa ctgtctgacc tgccacatcc ggaatggcaa
     2761 tggttgcagg agttctggat aagatccaaa taagagaagc tgccaccaat acaaggctgg
     2821 ccaacaaaat caggtaacgc atcttaaatc tatgtttttt cggtgcttgt ggttggtaag
     2881 tttcctctgt cacagcctgg cttgggtttt tgattgattt gtgttctgtc tgcgcttgaa
     2941 ccttaggaat agatgtcaag gtactctgag aaaccttcgg caaggtcttg gtatctgcct
     3001 tgctcgtttc atcaaagatt aacttacttt catttctacg attgtaggac aagctactag
     3061 acaagtccac atacatctct gaaaccgatc ggtagcgatt ggtcaacttt ttagcagttg
     3121 ccttgataat aacattttct aaagcctgag gtacagatgg attttctgca ataacggacg
     3181 gcaggggttt ctggaaatgc tggagggcaa tggtcaccgc gctatccccg tcataaggga
     3241 tatggcctgt caacatctca tagaaaataa tccccatggc atagatatca ctctgcacag
     3301 tcgccttaga accacgcgcc tgctctggtg acaagtaatg aactgagccc aacatcgagt
     3361 tagtctgggt cagacttgtc tctgcaaagg ctacagcaat cccaaagtct gtgaccttgg
     3421 cagtcccatc tggtgtcaaa aggatatttt gaggtttcaa gtccctgtga acaattcctc
     3481 gagtatgggc caagcgcata gccaagagaa tttgtcccat gatacggacg gcttcttcat
     3541 tagaaagagg ataatgttcc ttgatatagc gtttgaggtc tagtccagca acatactcca
     3601 ttgcaagata ctgttgaccg tcttcctcac caatatctgt tatccgaacg atatgaggat
     3661 ggtctagatc tgccatagct ctcgcttcac gctgaaaacg agctacagct atcgggtccg
     3721 tctggtagtt ggtcctcaga accttcactg ccacttcttc cccatctaag attaagtctt
     3781 tggctaggta aacatccgcc atgcctcctc gaccaatctg tttgacaatc cgatagcgtc
     3841 cggcaaaaat cttgccgatt tggatcattc ttcatcctcc tcgttcatag aaacaagggc
     3901 aaccgtaatg ttgtctaaac ctcctgcatt gttagcaaaa cgaacaagtg tctccgtttt
     3961 atctgctaaa ggaatatcac tggttacaat atcacgaatc tcactgcctg aaatcatgtt
     4021 ggtcaagccg tcactattga gcaagagata gtcacctgac tcaaggataa ctgtcccaaa
     4081 atcaggctga atttcatctt tttgcccaat agactgggtg ataatatttt tttgcggatg
     4141 agcttctgcc tcttctggtg tcaattgacc agccttgagc aattcattaa ccaaggaatg
     4201 atcgctcgtc aactgatggt attcttctcc acgaatcaag ccgatacgcg aatcaccaat
     4261 atgagcatag atagcctgat tatcaataat agcaaggact tccaaagtag ttcccatgcc
     4321 tctgtaagct tcatcctgac caagctggtg aatcttttga ttttcaattt ctaggtaatg
     4381 ggcgaaccat tcacgcactt cattgactgt atcgatctgg gtatcaaccc aagctacacc
     4441 caggtctgtg accgccattt cactagcgat attccctgca cgatgacctc ccatcccatc
     4501 agctaaaata atcatggtac gtccagctct attgacatag tggttgacat agtcttggtt
     4561 atttgttcgt ttctgaccaa catctgttaa taatgaaatt tccatgtgtc agttccttcc
     4621 taatccgata tcttgcgaaa ttgactgatg aagaatccat cacttccata caattcaggt
     4681 gtaatgagga tacagccgtc tttcatgata tccttacatt catgttctag ttttacctgc
     4741 tcgaactcgg gatgactctc taaaaaggcc ttaacgactt gaaaattctc ctctgagacg
     4801 atagtgcagg tgctataagt tattatacca cctttgccta gtatttgaca aacactacct
     4861 aatatttcta actgaatttc ctgcaaggac gcgaaatctg ccgtttcttt attgtatttg
     4921 atatctggtt ttcggcgcaa aagaccgatt cctgaacaag gagcatccac caaaatctta
     4981 tcaaaggaat cctggtcaaa aaactcatgc acctttctgg catccaattt ttgagtttga
     5041 acccgatctt caactcccag acgttgggca ttttcttgaa ttaaatccaa cttgtggtcg
     5101 tacaagtcca gagcagtaac ctgacctgtc gtaagataag aggctatatg ggctgttttc
     5161 ccacctggag ccgcacaggc atcaagcact cgctcatcac cttgtaaatc aagcgtcgga
     5221 gcaaccagct gactggactc gtcttggatg gtaatggctc catccgcaaa caaattatgc
     5281 cctgcaaaat gcccctgctc cttaaccaga ccagtggttg ctaaaaggga atcattcgcc
     5341 tccaacaagg cttggatttc ctcttttcga cttaggtctg ttacacgaat actggctttg
     5401 tttcgcacta acaggctttc aaagatggct tttgctctct cctctccgta ttcttccttg
     5461 agtttggcaa ctagccaaac tgggagagaa taggcaatgg agtcacgctt gttttttcgc
     5521 ttgatgctag caatatctgg ccagccttca cgcaagatac ggcgaaggag ggcattgacc
     5581 aatttttcac tgcctttttt acgaagtttg gccaattcca ctgcttcatt gaccacagca
     5641 tgatctggaa tcttgtccaa atagcggagt tggtaggcac tcatgagaag aaggacatag
     5701 agccagctgt ctaactggtc tctgtcttcg ataaagtggg ataggtacca ttccagagtc
     5761 agtttacggg ctaccgttcc atagaccagc tcggtcacta agcccttgtc tgctgccaaa
     5821 agttgactcc cctttagatg cttatttaag gcgatatttg agtatgcttg gttcacaaaa
     5881 acatcctcta gcactgctag agctaaactt ctagccgttt ctactttagt caccaaatcg
     5941 ttctcctaca gtcaatgtac gtccaactcc gttgaggaag gaagcaatgt ccatcttagg
     6001 cttaccagct ggctgcactt gtttgaggga tagagcccct tcagccgttg cgacaatcaa
     6061 ttctttcttg ccgatagaga gaatctcacc tggatttccc tgaccttcta ctggtagggc
     6121 ttcataaatc ttaaagcggt cgcccttaag gaaagcatgg gcaacaggcc aggggttcat
     6181 tccacgaatt tggttaaaga gttgacgatt ggttttgttc cagtccagtt tttcttcctc
     6241 tggctttata tttggagaga aggtaacctg actcgtatcc tgcggttcag gtttgatatc
     6301 accagcaata taggcaggca gagtgtccaa aagcaaatca cgaccaacta gcgccaattt
     6361 ttcaaacaag gtgccaacat tgtcctcatc tgtgatcgga atgctgcgac gagaaatcat
     6421 atctcctgca tccatttcct taaccatttc catgatggtc acaccagctt cctcatcccc
     6481 ttgaatcaag gcataatgga taggcgcacc accacggtgt ctaggaagga gggaggcatg
     6541 aacgttgaca gcaaagtcca tgctatcaag gagtttgctt gggagaaact gcccaaaagc
     6601 agcagtcaca attccatctg ctcctagctt cataagttct tccatctctg gacttccaga
     6661 taatttttca ggttggtaga tagatagtcc tgcttccttg gcagcctgct tgactggggt
     6721 ttcttggata acttttttac gaccaacagc acggtctggc tgggtcacaa cggctagaat
     6781 ttcgtaacgg tcatctgtca aaagtccttt taagactgtt gctgaaaagt cgggggtccc
     6841 cataaagatt agttttgtca tatcttctcc ttcttataaa aattgctgcg gctcatggtc
     6901 aatgctgaga cggagctcac tattttcccg ttcttgagtc aaggccaaga cctggttgag
     6961 ggtcggcccc agctcatctt ctaaacggta tttaattaaa atctggtaat gatagaggtt
     7021 gtgggtacgg gcaatcggtt ttggcgttgg ccccagaatg ggactggtct ctgacaagcc
     7081 tgaccgcaaa atgttcatga cttcataggc acgtttgaaa acctcttctt ctttcttgtg
     7141 agaaagggta ataccaatcg tgaaatagta aggtggatag ccgagttgtc gtctgattcc
     7201 catttcatag gcataaaagc cttcgtaatc ttgatccttg gcaaatcgaa tagcatagtg
     7261 ctgcgaattg taggactgta tcaagacttg acctgctttt tcagcccgac ctgctcgacc
     7321 tgccacctga gtcaagagct ggaaggttct ctcagaagaa cggaaatcag gcagattcaa
     7381 ggccgtatcc gcatttagaa ctccgactag ggtaacattg ggaaaatcca aaccctttgc
     7441 aatcatctga gtaccaagta aaatatccgc ttcccctcgc ccaaactggt caagcaaggc
     7501 ttggtgactg cctttctttc gagtcgtatc cacatccatc ctcaaaatgc gagcttgggg
     7561 aaagagttct gctagctcat cataagcctt ctgagttccc gtcccatagt aacgaatact
     7621 gcggctctta cagttaggac agacctgagg aatatccttc gagaaaccac aataatggca
     7681 gttcatagtc ttggtatcca tatgcaaggt cagagaaata tcgcagttgg gacaagtatc
     7741 caccgtccca cactcccgac acatgacaaa gctagaataa ccacggcgat tgagcatgag
     7801 aaccacctgc tcttttttaa ccagacggtc ttggatagcc tctagcaaag gaggcgtaaa
     7861 gtttgacgtc tcattttgtc cgatatagtc tcgaaagtca atcacttgaa cctcagggat
     7921 tgtagccaaa ggattggcac gttgggttag acgtaagtgt tgatagacgc ctttgccagc
     7981 acgtgcccgg ctctctaagc tcggcgttgc agatccaagt accagagttg cttgattata
     8041 ctgagcccgt aaaatagcta cctctctggc atggtaacgg ggattgctgt cctgcttata
     8101 agccgcttca tgctcttcat caataatcat gacacccaga tttttcagag gagcaaagat
     8161 agcagatctg gcaccaacaa caacttgggc atcgccacgc tccaccttgc gccattcatc
     8221 atacttttca ccattggata atcctgagtg aagaatggct accttgtccc caaaacgtgc
     8281 tataaaacgc tcggtcatct gaggagtcaa ggaaatctca ggtaccagca aaatagctgt
     8341 cttgccctta tccagggcac cttggataat ctgcaagtaa acctcggtct tcccacttcc
     8401 tgtaatccct tgaagtagaa agggaggttg agaactgcca atagaactca caaccgcatc
     8461 acgcgcctgt ctttgttctg gatttaactc caaaggtcta cttgcttcaa ttccttcaaa
     8521 ataagcagcc gagcgttgaa cttccttttg gactatggta acagcacctt gatccacaaa
     8581 gaagttgact tgctctcgcg agtaggactc taacaagcta gccaaggaag cgctctctgg
     8641 atgagacagc agataatctc tcagttccaa ctttttcttg gcacgtgtag aaatctcaac
     8701 accttctaat tgggcatggt caacctcata ccaagactgg gtcttgacct tcttttgatc
     8761 gactgcctga tattccagac caagcaggcc ttttctagtc aaacgcatca tttcagcttg
     8821 cttggcaagg tctagtgaag aaaaggctag cgaatcttct gaaccaaaca ggcgcactcg
     8881 ttcttcctga ctcaagcctt ccagaggata gagaatcttg tcatagctag aattcagaaa
     8941 ccctggaagc atggccttga ggatagagat tttgtaggag aagacagatt tgcgtaactc
     9001 ctcagccagc cagagttgtt ctggcgtgag aacaggagaa aaatccagca cctctgcaat
     9061 atcttttaaa tcttgctcca tctcttctcc atctgattgg gtcttcaaac caagaacaat
     9121 cccttgaatc aggcgattac ccttaccaaa aggcacatga acccgcatcc caacttccag
     9181 cattccctca aattcctccg gaatcctgta actatagggc tggtccgtct gcatcaaggg
     9241 cacatctacg ataatcttag ctagggccat cttctcacct cctccttgtc agcacattct
     9301 tgcaatagaa aaaataagat tgagtccccc caaccttaaa ttttttcacc atcttctttt
     9361 tctttagcaa tttgctcttt gattttcttt tcttcttctt ctttgcggcg tttttcttct
     9421 tcgatacggc gacgcactgc ttcacgtttt ccttctggat ctgggtgaat tgtaacgttt
     9481 cctgattcga tttcttctaa agcgcgaaga gttgattttt cagacttgaa accttgagtt
     9541 gctggggcac ctgcttccaa ttcgtgggca cgttttgctt ccaagattac gagtgaatat
     9601 tttgaaggaa ccttgtcgag caaggtatca atagagggtt ttaacatcat ttgcttgtac
     9661 ctattttcta aattttatcg ggtagttgga gattttggta acatctcctg atagtgacca
     9721 atgacacgat ccacacagaa gtgttctgct tcaatcacac atttgacacg ttcagcagct
     9781 aggggcacct gatcgttgac aatcgcataa tcatactcac gcatgagggc aatttcttcc
     9841 ttggcctttt cgattcgttg ggcaatcact tctgcactat ctgttccacg acctaccaag
     9901 cgatcttgca attcatccaa atctggtggt gtcaggaaga taaagacagc atctggaacc
     9961 tttttcttga cctgaagagc accctgaact tcaatttcaa ggaaaacatc gattcccttg
    10021 tccaaggttt cattgacata ggtcagagga gttccatagt agttaccgac atattctgcg
    10081 tattccaaca tctgtccttg acgaatcagc tcttcaaatt cttcacgagt acggaagaaa
    10141 tagtcaacac cgtccacttc tccaggacgt tgtgcgcgtg tcgtcatcga tacagaatat
    10201 tgaaattggt tttcagaact ctcaaaaatc tctcttctaa ccgttccttt tccaacccct
    10261 gaaggaccag aaaaaacgat tagtaagcct cggtctgcca ttgtgtctcc ttttagtcaa
    10321 tctgtgaaat aacatttctc tagaataatg gcaaaaagcc agattatcct ttacagtctt
    10381 tctatctagt gtaacaaaaa agcagtaatt tttcaactgc tctttcttat ttatttagca
    10441 taatctactg cacgaagctc gcgaatcacg gttaccttga tatttcctgg ataatcgaga
    10501 ttgttttcaa ttttcttacg aactttgtga gccaagattg tgactttgtc gtccttgatt
    10561 tttcctggat tgaccatgat acgaatttca cgtcctgctt ggagggcaaa gctagtttgc
    10621 actccttcaa agccgttagc aatttcttcc aaatcatgga gacgcttgat gtagctttca
    10681 agagactcac tacgagcacc tggacgggct gcgctcaagg catctgctgc agcgacgata
    10741 actgctatca cgctctcagc ttcaacatct ccgtggtgac tagcaatcgt attcaccaca
    10801 actgggggtt ccttgtactt acgggccaat tccataccga tttcaacgtg gctaccttca
    10861 acctcatggt caatggcttt cccgatatcg tgaaggaatc cagcacgacg ggcaagagcc
    10921 gcattttcac caagttcgct cgccatgata ccagccaact tagcaacctc aatcgaatgg
    10981 cgcaaaacat tttgtccata tgaagtacgg aactgcaaac gtcccataat cttcatcaag
    11041 tctggatgaa ggtttggcgc accaatttca taggcagcag cctcaccgta ttcacgaatc
    11101 ttattgtcaa tctcttgacg gtttttctca accaactctt cgatacgagc tggatgtata
    11161 cgaccatctt tgagcaacat ttccatagtc atacgggcaa tctcacgacg aatcggatca
    11221 aatcctgaca aggtcaccac ttctggtgta tcgtcgataa tcacatcgac ccctgtcaaa
    11281 ctttcaaagg tacgaatgtt acgaccttca cgaccaataa tgcgtccctt catagtatcg
    11341 tctggcagat gaactgttga gtttgttgac tccgctacat attcaccagc gatacgttgc
    11401 atagcttgaa ccaagatgtc cttggccatt ttgtcagaac gttccttgac ctcttgctca
    11461 gcttcgcgaa tgcgactggc aatctccctg gtcaagtttt cctctgtctg agccaagata
    11521 atatctcgtg cttctgcctg agacagagca ccaatacgct ctagttctgc ttctttttgt
    11581 ctttcgactt cctctaattg ctcttcacgc gcatcaaggt ttttcgctct atcagaaata
    11641 ctttgttctt tttgttcaag tgtttgttct ttactcgtca aattgtcgtc cttacggtca
    11701 aggctagtag ctctctctgt caaacgactt tcgatttgtt tgagttcttg acgttctgat
    11761 ttgaattcag cgtccacttc ttcacggtat tttctggctt cttctttggc ctccaatagt
    11821 gcttcttttt taagagactt gctttcacgt ttggcttcat taacaagtaa atccgcttca
    11881 cgctcagctt gtccacgtaa attagttgct tcttgttcag catttaaaag catcaactct
    11941 gcagcttcct gagatgattt catcttagct gagatgctga catatccaat gactaaacca
    12001 atgatgacgg caaaaacagc aatcgcaagc gacatgattt ccatgttttt acctcatttt
    12061 attgttattc cgaatgacat acattctttt acattctacc ataaaaaagt gattttcaca
    12121 aacctaaaat agaatatgtt ttgaggaaat tagtctgaat tttaggatta cacttccttg
    12181 gttttttaag gagataagtc agtgccgcta tcaaaaagtt ccaccgaatt tctaccgcta
    12241 tggattttct attgttgtgc caaatagtgt tccactagat tcaaacattt tgctttcaag
    12301 tctttgaaaa ttcactttag gaaatcacag cttatcttca agatcaaatt tgtcataaat
    12361 taactttttt tatttagttg tatcaattta tttcttaagt attaatttat cctcatcgaa
    12421 agcttttaaa ata
//
LOCUS       DAVMEI010000050        12380 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVMEI010000050 DAVMEI010000000
VERSION     DAVMEI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12380
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.050"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            543..2222
                     /locus_tag="VYQ24_001053"
     CDS             543..2222
                     /locus_tag="VYQ24_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1968203.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGE
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPAYG
                     NGGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELDYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GSALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFSRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGEALHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            complement(2262..4346)
                     /locus_tag="VYQ24_001054"
     CDS             complement(2262..4346)
                     /locus_tag="VYQ24_001054"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1968204.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTGQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQERGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            4517..5797
                     /locus_tag="VYQ24_001055"
     CDS             4517..5797
                     /locus_tag="VYQ24_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1968205.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEKNWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETGQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSESIIADTKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            5888..8533
                     /locus_tag="VYQ24_001056"
     CDS             5888..8533
                     /locus_tag="VYQ24_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1968206.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKDHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLSRQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            8617..9486
                     /locus_tag="VYQ24_001057"
     CDS             8617..9486
                     /locus_tag="VYQ24_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1968207.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQTCTYHADANLY
                     GALVNWLQEEKQW"
     gene            9480..11360
                     /locus_tag="VYQ24_001058"
     CDS             9480..11360
                     /locus_tag="VYQ24_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1968208.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGSETAQFSILSSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINSNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            11738..12100
                     /locus_tag="VYQ24_001059"
     CDS             11738..12100
                     /locus_tag="VYQ24_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968209.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSLEQYPDYDGFDYEEW"
     gene            complement(12170..>12380)
                     /locus_tag="VYQ24_001060"
     CDS             complement(12170..>12380)
                     /locus_tag="VYQ24_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEV1968210.1"
                     /translation="PRKSSKLKPLTVEDKTYNHALSKDRSKVENILAKVKTFKMFSTT
                     YRNHRKRFGLRMNLSAGIINHELGF"
BASE COUNT         3510 a         2516 c         2978 g         3376 t
ORIGIN      
        1 ttttttaaaa aatttttcaa aaaatataca aatgaccaaa aagtgtccaa ttatcatctg
       61 gttatcactt ggatctgtat aaagagaaat gtttgtgtga aaggataaaa agatgcaaca
      121 ttcttttgaa gatagatttt ggagaagcat ttcttgttca attgtacaaa ttatagaatc
      181 ttttctgagt atttaggaag agataattat cttattgacc ctctttttat tttttgttat
      241 agtcgacgag gtgatagctt agaataatca cttattcaca atctgtggaa aagtctggag
      301 agtgaatggt cttcacacaa tgacctgaaa gaagcctgag aataattatg gagagtagca
      361 ttctgagagg tgttagcaga atcatatgac agagctgttt gaagagggaa tattgaggag
      421 aaaaatcctg agcctaccag ttggagttgg aaagagctgg ctgttagatc atggtttatt
      481 atccacaacc tgtggataac cttgtgaaca agagaagttg ctaaagaagg agatatataa
      541 cgatgaagaa aatcaaaccg catggaccgt taccaagtca gactcagcta gcttatctgg
      601 gagatgaact agcagctttt atccacttcg gtcctaatac cttttatgac caagaatggg
      661 ggactggaca ggaggatcct gagcgcttta acccgagtca gttggatgcg cgtgagtggg
      721 ttcgtgtgct caaggaaacg ggcttcaaaa agttgatttt ggtggtcaag caccacgatg
      781 gctttgtcct ttatccgaca gctcacacag attattcggt taaggtcagt ccttggagga
      841 gaggagaggg cgacttgctc cttgaagtat cccaagctgc cacagaattt gatatggata
      901 tgggggtcta cctgtcaccg tgggatgccc atagtcccct ctatcatgtg gaccgagaag
      961 cggactacaa tgcctattat ctggctcagc tgaaggaaat cttatcaaat cctgcctatg
     1021 gaaatggagg gaaatttgct gaggtttgga tggatggtgc cagaggagag ggcgcgcaaa
     1081 aggttaatta tgaatttgaa aaatggtttg aaaccattcg tgacctgcag ggcgattgct
     1141 tgattttttc aacagaaggc accagtatcc gctggattgg caatgaacga gggtatgcag
     1201 gtgatccact gtggcaaaag gtgaatcctg ataaactagg aacagaagca gagctggact
     1261 atcttcagca cggggatccc tcgggcacga ttttttcaat cggagaggca gatgtttcca
     1321 tccgtccagg ctggttctac catgaggatc aggatcctaa gtctctcgag gagttggtcg
     1381 aaatctactt tcactcagta gggcgaggaa ctccactctt gcttaatatt ccgccgaatc
     1441 aagctgggct ctttgatgca aaggatattg aacgacttta tgaatttgcg acctatcgca
     1501 atgagctcta taaagaagat ttggctctgg gagctgaggt atctggttca gctctttccg
     1561 cagactttgc ttgtcgccat ttgacagacg gccttgagac cagctcttgg gcaagcgatg
     1621 cagacttgcc catccagtta gaactcgact taggttctcc taaaactttt gatgtaattg
     1681 agttaagaga agatttgaag ctagggcaac gaatcgctgc ttttcatgtg caagtagagg
     1741 tggatggtgt ctggcaggaa tttagcaggg gcttcacagt tggtcataag cgcctcttgc
     1801 gaggtccgct agtagaggca caaaaggtgc gcgtgatgat taccgaggca caatctattc
     1861 ctgtattgac caagatttcc ctttataaaa ctcctggatt atcaaaaaaa gaagttgttc
     1921 aggaactagc atttgcagaa aaaagcctag ctgtggcaaa gggagaggct cttcatttta
     1981 ggattgaacg tagtgaaagt agctcctctt tagaggctaa gatttcgatt caaccgggga
     2041 caggtgtcca tggtgtcgcc tatcaggacg agatttatgt ccttcaattc caagcagggg
     2101 aaagcaaaaa agatttgcat ctaccaaccc tgtatttcgc aggagataaa accttggatt
     2161 tctatctgaa cctaacggtg gatggtcagc ttgtggatca acttcaagtc caagtttcat
     2221 aaaagaagaa cctttgcgcg atgcaaaggt tcttttggtt attagtgact tggtagccag
     2281 ctgagggtga aagttagttg ttcagctttt aagaggtctt ggtgttgaat agttgatacg
     2341 agtgttttgt tcagtcggca ttctttgaca aagttaaaat ggttgtggtt ttgtttagta
     2401 tggatatcca gccatttatc ttctttagct aggtagactc gtagatggtc aaagagaggg
     2461 attccgaggt catagcttgg ttttcctgga caggttggat aaaatccgag agctgaccag
     2521 atgtaccaag cagagagact accattgtct tcatctccag gataggcttc ccaacttggg
     2581 tgaaaagctt tctgacggag cgtcttgatg agaagggcag tgtagtcagg gtaatcgctg
     2641 tagcggaaga gataaggaat gtggaaacta ggctggttgg aaatggctat ttgtccaaaa
     2701 ggagcagtag ccatctcgct catttcgtga atttcgtaac catagcctgt tgtttcaaag
     2761 aggggagcat cttgacaggc tttcaaaaga tagttgctaa aggtttcttt tccacccata
     2821 agttggatta agccagggat gtcgtggaga acgcctaaag tagcttgaat ggcagagcat
     2881 tcagcgtagt ctcgccccca actataagga gagaagtcag ggtgaaagtt tccttgattg
     2941 tctcgtgctc gcatgtaacc tgtctcagcg tcaaatagct ggcggtaatt ttgtgaagca
     3001 gccttgtagg tttcagcgat ttctatgttc tctagttttt tggcacagct ggcgatacaa
     3061 aagtcactat aggcatagtc tagagtatgg ctaacacttt cgtggtggtc ggtagagagg
     3121 taacctcgtt cttggtattg ggctagtccg tggcggccat tgatgccgag agggtcggct
     3181 ttgctggctg tttcgagcat ggcttggaag agttctcctt ctaggtcagg ggccatgtcc
     3241 ttgcaggcgc tatctgcgat aataccgtct aaaagtgtac ctggcatcat accccgttca
     3301 tctggagcca gccattttgg aaggaaacca gtatcgcggt agctattgag gaaaccttct
     3361 aaaaagcgtt gatagtgctc cggtatgata agggcaaaga gggggaaggt ggtgcggaag
     3421 gtatcccaga aaccattgtt gctaaagagg acaccaggct tgacagtacc agtagccaga
     3481 tccatgtgga tggcttgccc tgactcatca acctcataaa aagtctgtgg gaagaggaag
     3541 agtctgtaga ggcagtggtc aaagaaggtt cggtcagcct ctcctatctc tataatgtca
     3601 aaacgatgga ggagattttc ccaatccgct tgggcacttg atttacagct atcaaaatct
     3661 tcttgaggta gattaagcag tgcttgagaa ggagagatga aagaagtggc tagttgcatc
     3721 tcggtttgac tacttgctaa gtcaattcgc cagtccccag cttcttggct gatagcaaga
     3781 atatccgtgt tcatttgcag ggcagtgaac atcgttagcg aatttttgtt ggtttcagtt
     3841 ttaccttctt gtcgcagggc aagagttcgc ttatctactt gctctactgt cagttcatct
     3901 gctgcgtgaa gatagaggga gagggctttg ccttgctttt gcttcaaacg aatagaagca
     3961 ccgtagcaag tcggtgtgag ctgggtttca atctgatagc gcagggagaa aatctttaga
     4021 taatgaggtt ggaaagaggc cttatcccta tcataggaag attggcggtg aaagagactg
     4081 tctcccccaa gctgacctgt gacaggtgtc agaaggagcc aagagtagtc cccaatccaa
     4141 ggactgggct ggtgagttaa tcgaatcccc tgaaagatag gcagatgcgg atcaaaaaac
     4201 caagatccat cctggtcact ggtctggggc acaaagtaat tcatcccaaa aggcacgcct
     4261 gtgtatggca gggtatttcc ccgagaaaag gcatgcttgt tggtagttcc aaagcgggta
     4321 tcgatggttt caagtagtgg tttcatagtc tttcctttag ctgtttttct acattatatc
     4381 agaaaaagag ggcctttaga aaaggagttt caaaaataac tattttgtag aaaaatgact
     4441 atcatttgtg tatgtatttt ctgtctcaaa agctatatac tgaacatgaa acttgtttga
     4501 aagagggaat tgcacgatga tttattcgaa agaaattgtt agagaatggc tagatgaagt
     4561 agcagagcgg gctaaggacc atccagagtg ggtggatgtt ttcgagcgtt gctacaccga
     4621 taccttggac aatacggttg aaatcttaga agatggttca acttttgtct tgactgggga
     4681 tattcctgcc atgtggcttc gagattcgac agcccaactc agaccctacc ttcatgtagc
     4741 taaaagagat gccctcctgc gtcagaccat tgcaggtttg gtcaaacgtc agatgacctt
     4801 ggtactcaag gatccctatg ctaactcctt caacattgag aagaactgga aagggcacca
     4861 cgagactgac cacacagacc ttaacggctg gatctgggag cgcaagtatg aggtggattc
     4921 actttgctat cctttgcagt tggcttatct cctctggaaa gagactggcg agactggtca
     4981 gtttgatgag acttttgtcg cagcgactaa ggaaattctc catctatgga cagttgaaca
     5041 agaccacaag aactctcctt atcgttttgt ccgagatacg gaccgtaagg aagacacctt
     5101 ggtaaatgat ggctttggac ctgactttgc agtgacaggt atgacttggt cagcctttcg
     5161 tccgagtgat gactgttgcc agtatagtta cttgattccg tcaaatatgt ttgctgtagt
     5221 agtcttgggt tatgtgcaag aaatcttcgc agcattaaac ctagctgata gcgagagcat
     5281 tattgctgat accaagcgtc ttcaggatga aatccaagaa ggaatcgaaa actacgctta
     5341 caccaccaac agcaagggcg aaaagattta cgcttttgaa gtggatggcc taggaaatgc
     5401 cagcatcatg gatgatccaa atgtaccaag tctactagct gcgccctatc tgggctactg
     5461 ttcggtcgat gatgaagtgt atcaagctac tcgccgtacc attttgagct ctgaaaatcc
     5521 atacttctac caaggagaat acgctagcgg tctcggaagt tctcatacct tctatcgcta
     5581 tatctggcca atcgcccttt ctatccaagg attgacaaca agagataagg cagagaaaaa
     5641 attcttgctg gatcagctgg ttgcctgcga tggtggtaca ggtgtcatgc acgaaagctt
     5701 ccacgtagat gatccgaccc tctactctcg tgaatggttc tcctgggcta acatgatgtt
     5761 ctgtgagttg gtcttggatt acttggatat ccgctaaggg gctcgcttta gctcaaccga
     5821 ttcttatcag aatcacaagt ttacatttaa aacgttaaaa tttaaattta gaatgaggtt
     5881 ttacttcatg gaaaatgttg ttgtacatat tatctcacat agtcactggg accgtgagtg
     5941 gtacttgcct tttgaaagcc atcgtatgca gttggtggaa ttgtttgaca atctctttga
     6001 tctctttgaa aatgaccctg agttcaagag tttccacttg gatggacaaa ctattgtcct
     6061 tgacgactac ttacaaattc gccctgaaaa tcgtgacaag gtccaacgct acattgacga
     6121 gggcaaactt aaaattggtc ccttttacat cttgcaggat gactacttga tctccagtga
     6181 agccaatgtc cgcaatacct tgattggtca acaagaagct gccaaatggg gtaaatcaac
     6241 ccagattggc tactttccag atacctttgg aaatatggga caagcgcctc aaattcttca
     6301 aaaatcaggc attcacgtgg cggcctttgg tcgtggtgtg aagccgattg gatttgacaa
     6361 ccaagtcctt gaagatgagc agtttacatc ccagttttca gaaatgtact ggcagggtgt
     6421 ggacggtagt cgtgttttag gtattctctt tgccaactgg tacagtaatg ggaatgaaat
     6481 cccagttgac aaagatgagg ccttgacctt ctggaaacaa aaattgtcag atgtgcgtgc
     6541 ctacgcttcg accaaccaat ggttgatgat gaacggctgt gaccaccagc ctgtccagaa
     6601 aaatctgagc gaagccattc gtgtggcaaa tgaactcttc ccggatgtaa tctttgttca
     6661 tagttctttt gatgaatatg ttcaagctgt agaaggtgcg cttcctgaac acttatcaac
     6721 tgttacaggt gagttgacca gtcaggaaac agatggctgg tacacacttg ccaacacttc
     6781 ttcatcccgc atttacctaa aacaagcctt ccaagaaaat agcaacctcc tagagcaagt
     6841 ggtagaaccc ttgactatta tcactggtgg acacaaccac aaggaccagt tgacctatgc
     6901 ttggaaaaca cttttgcaga atgcgccaca tgatagtatc tgtggctgta gcgtggacga
     6961 agttcaccgc gagatggaaa cgcgttttgc caaggtcaac caagtaggaa actttgttaa
     7021 aagtaatttg ctcaacgagt ggaagggtaa aattgctacg gataaggctc aaagtgacta
     7081 tctctttact gtcattaaca caggcttgca tgataaggtc gatactgtca gcacagtgat
     7141 tgatgtggcg acttgtgatt tcaaggaatt gcacccaaca gaaggctaca aaaagatggc
     7201 tgctcttatc ttgccaagtt accgtgtgga ggacttggat ggtcgtcctg tagaggctac
     7261 aatcgaagac ctcggagcta attttgaata cgatttacca aaagacaagt tccgccaagc
     7321 tcgtattgct cgacaagtgc gcgtgaccat tccagttcac ctagcaccgc tttcttggac
     7381 aaccttccaa ttgctggaag gagaacaaga acaccgtgag ggtatttacc aaaacggagt
     7441 gattgataca ccattcgtaa cggtgagtgt ggatgacaac atcacagtct atgataagac
     7501 aactcacgaa gcctatgaag actttatccg ctttgaagac cggggtgaca tcggaaacga
     7561 gtatatctat ttccaaccaa aaggaacaga gccaatcttt gcagagctta aggaccacga
     7621 ggtcttggaa aacacagatc gctatgctaa aatcttgctc aaacatgaat tgaccgtgcc
     7681 tgtcagtgcg gatgaaaagc tagaagaaga gcaacaaggt atcatcgagt ttatgaagcg
     7741 tgaggctgga cggtcagaag aattgacaaa cattcctctg gaaactgagt tgactgtctt
     7801 cgttgacaat ccacaaatcc gcttcaagac tcgctttact aacactgcca aggatcaccg
     7861 tatccgtctc ttggtcaaga ctcataacac gcgtccaagc aatgattctg aaagtatcta
     7921 tgaggtggtg acacgaccaa acaaaccagc tgcttcatgg gaaaatcctg aaaatcctca
     7981 acaccaacaa gcttttgtca gtctgtatga cgatgaaaaa ggggtgactg tatccaacaa
     8041 gggattgaat gaatacgaaa tccttgggga taacaccatt gccgtgacca ttttgcgtgc
     8101 atcaggtgag ctaggtgact ggggctactt cccaacgcca gaagcacaat gcttgcgtga
     8161 gtttgaagtc gaatttgcga ttgaatgcca ccaagcccaa gaacgcttct cagcctatcg
     8221 tcgtgccaaa gccttgcaga caccgtttac cagccttcag ctttctagac aggaaggaag
     8281 cgtggttgcg actggtagcc tcttgagcca ttctgttctc agcataccgc aagtttgtcc
     8341 aacagccttt aaagtggcgg aaaatgaaga aggctatgtc ctccgttact ataatatgag
     8401 tcaagaaaat gtgcgcatat cagaacatca acaaaccatt cttgacttac ttgagcgacc
     8461 atatccagtt cattcaggac tattggctcc acaagagatt cgtacagaat tcatcaaaaa
     8521 agaagaaatt taatttcaaa aagtaaacat caaaagaaag gaggggcgaa aaggtaagaa
     8581 ctaactgctg attcgcccct ttttatggta aaaacaatga ccattgcaac gattgatatc
     8641 ggagggactg ggattaagtt tgccagtctg actcctgatg ggaaaatact ggataagaca
     8701 agtatttcaa cgcctgaaaa cttggaggat ttactagcgt ggctagatca acgcttgtca
     8761 gaacaggatt acagtgggat tgctatgagc gttccaggtg cggtcaatca agagacaggt
     8821 gtgattgatg gcttcagtgc ggtgccctac atccatggct tttcttggta tgaggcgctt
     8881 agctcttatc agctacctgt ccatttagaa aatgatgcca actgcgttgg actcagtgaa
     8941 ctactagctc atccagagct tgaaaatgca gcctgtgtcg tgattgggac agggattggc
     9001 ggagccatga ttatcaatgg tagacttcat cgaggtcgcc acggcttggg tggagaattt
     9061 ggctacatga caacccttgc ccctgctgaa aaacttaata actggtcgca actagcatca
     9121 actgggaata tggtacgata cgtgattgaa aaatctggtc atactgattg ggacggtcgc
     9181 aagatttacc aagaggccgc agctggtaat gctctttgtc aagaagccat tgagcgcatg
     9241 aaccgcaatc tggcgcaagg cttgctcaat atccagtatc tcatcgatcc agatgtcatc
     9301 agtctgggtg gctctatcag tcaaaatcca gattttatcc aagatgtcaa gaaggctgtt
     9361 gataactttg tcgataccta cgaagaatac acggtcgcac cagttatcca gacctgcacc
     9421 tatcacgcag atgccaatct ctacggtgcc cttgtcaact ggctacagga ggaaaagcaa
     9481 tggtaagatt tacaggactt agtcccaaac aaacgcaagc tattgaggtt ttaaaaggtc
     9541 atatttctct accagatgtg gaagtggctg tcactcagtc tgaccaagcc tctatctcta
     9601 tcgagggtga ggaaggtcac tatcaattga cctaccgcaa acctcaccaa ctttatcgtg
     9661 ccttatcctt gttggtaaca gttctagcag aagctgataa agtagagatt gaggaacaag
     9721 cagcttacga agatttggct tacatggttg actgttctcg aaatgcggtg ctgaatgtgg
     9781 cttctgccaa gcagatgatt gaggtcttgg ctctcatggg ctactcaacc tttgagcttt
     9841 acatggaaga cacttaccag attgaagggc agccttactt tggctatttc cgtggagctt
     9901 attcagcaga ggagttgcag gaaatcgaag cctatgctca acagtttgac atgacctttg
     9961 taccatgcat ccagaccttg gcccacttgt cggcctttgt caaatggggt gtcaaggaag
    10021 tgcaggagct ccgtgatgta gaggacattc ttctcattgg cgaagaaaag gtttatgacc
    10081 tgattgatgg catgtttgcc acgttgtcta aactgaagac tcgcaaggtc aatatcggga
    10141 tggacgaagc ccacttggtt ggtttgggac gctacctgat tttgaacggt gttgtggatc
    10201 gtagtctcct catgtgccaa cacttggagc gcgtgctgga tattgctgac aaatatggtt
    10261 tccactgcca gatgtggagt gatatgttct tcaaactcat gtcagcggat ggccagtacg
    10321 accgtgatgt ggaaattcca gaggaaactc gtgtctacct agaccgtctc aaagaccgtg
    10381 tgactctggt ttactgggat tattatcagg atagcgagga aaaatacaac cgtaatttcc
    10441 gcaatcatca caagattagc catgaccttg catttgcagg gggagcttgg aagtggattg
    10501 gctttacacc tcacaaccat tttagccgtc tagtggctat cgaggctaat aaagcctgcc
    10561 gtgccaatca gattaaagaa gtcatcgtaa cgggttgggg agacaatggt agtgaaactg
    10621 cccagttctc tatcctatca agcttgcaaa tctgggcaga actcagctat cgcaatgacc
    10681 tagatagttt gtctgcgcac ttcaagacca atactggtct aacggttgag gattttatgc
    10741 agattgacct tgccaacctc ttaccagacc taccaggcaa tctcagcggt atcaattcca
    10801 accgctatgt tttttatcag gatgttcttt gtccgatact tgaccgacac atgacacctg
    10861 aacaggacaa accgcacttc gctcaggctg ctgagacgct tgctaacatt aaagaaaaag
    10921 ctggaaacta tgcttatctc tttgaaactc aggcccagtt gaatgctatt ttaagtagca
    10981 aagtagatgt gggacgacgc attcgtcagg cctaccaagc ggatgataaa gaaagtttac
    11041 aacaaatcgc cagacaagaa ttaccagaac ttagaagcca aattgaagac ttccatgccc
    11101 tctttagtca ccaatggctg aaagaaaaca aggtctttgg tttggataca gttgacatcc
    11161 gtatgggcgg actcttgcaa cgcatcaaac gagcagaaag ccgtatcgag gtttatctgg
    11221 ctggccagct tgaccgcatc gacgagctgg aagttgaaat cctaccattt actgacttct
    11281 acgcagacaa ggatttcgca gcaactacag ctaaccagtg gcataccatt gcgacagctt
    11341 cgacgattta tacgacttaa tactcttcga aaatctcttc aaaccacgtc agcttccatc
    11401 tacaacctca aaacagtgtt ttgagcagcc tgcagctagc ttcctagttt gctctttgat
    11461 tttcattgag tataaaaaca agaacacctt gcttggcgca gggtgtttcg cgtgaaacag
    11521 aagaattatc tggtttcaaa tgctacagtt agacaaactt atgataaaat agcagaaagt
    11581 gaatgtttcc taagagcaat tctatcgatg agcaattgga ggtattatgc tacacttaaa
    11641 attagtaaaa caagaaatag aagctgaaaa gccagcatct gtagaagctt ggatcatttc
    11701 cgtcaaattt taaaaaggtt gctaccgaca tatatagatt ccaaaaacaa aaacgttagc
    11761 ggaactagca gatgtgattt tatggagttt tgattttgca aatgatcatg ctcacgcatt
    11821 tttcatggat aatgttgagt ggagtcatgc agattcttac tttcttagct ttgttagtga
    11881 cgatgttgaa gaacgttaca cagaaaatgt ctatctggat agcctaagtg tcaaacaaaa
    11941 atttaagttt attttcgact tcggtgatga atggcgtttt gaatgccaag tgctgagaga
    12001 aatcgagaca gaggacgaag aagcttatct cgtacgttcg gttggaacgt cgctagaaca
    12061 atatccagat tatgatggtt ttgactatga agaatggtaa aattgaaatc taaaaaacaa
    12121 ttggaatcag tctgtgtagg cttagtattt caatagactt cctgcaaaac tagaatccta
    12181 gttcatgatt gataatacca gcactcaaat tcattcgtaa tccaaagcgt ttacgatgat
    12241 ttcgataggt tgttgaaaac attttaaacg tttttacttt ggcaaggatg ttctcaacct
    12301 tgcttctatc cttagatagt gcatggttat aggttttatc ttcaactgtt agtggcttga
    12361 gtttgctgga tttacgtgga
//
LOCUS       DAVMEI010000051        12355 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVMEI010000051 DAVMEI010000000
VERSION     DAVMEI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.051"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            16..383
                     /locus_tag="VYQ24_001061"
                     /pseudo
     CDS             16..383
                     /locus_tag="VYQ24_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
     gene            518..1477
                     /locus_tag="VYQ24_001062"
     CDS             518..1477
                     /locus_tag="VYQ24_001062"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1968211.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            1574..2413
                     /locus_tag="VYQ24_001063"
     CDS             1574..2413
                     /locus_tag="VYQ24_001063"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1968212.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(2461..2631)
                     /locus_tag="VYQ24_001064"
     CDS             complement(2461..2631)
                     /locus_tag="VYQ24_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1968213.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            2752..3636
                     /gene="miaA"
                     /locus_tag="VYQ24_001065"
     CDS             2752..3636
                     /gene="miaA"
                     /locus_tag="VYQ24_001065"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1968214.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            3629..4867
                     /gene="hflX"
                     /locus_tag="VYQ24_001066"
     CDS             3629..4867
                     /gene="hflX"
                     /locus_tag="VYQ24_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1968215.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            4860..5483
                     /locus_tag="VYQ24_001067"
     CDS             4860..5483
                     /locus_tag="VYQ24_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1968216.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            5498..6427
                     /locus_tag="VYQ24_001068"
     CDS             5498..6427
                     /locus_tag="VYQ24_001068"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1968217.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTGASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            6448..7203
                     /locus_tag="VYQ24_001069"
     CDS             6448..7203
                     /locus_tag="VYQ24_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1968218.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEPDAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(7257..8165)
                     /locus_tag="VYQ24_001070"
     CDS             complement(7257..8165)
                     /locus_tag="VYQ24_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1968219.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            8229..8498
                     /locus_tag="VYQ24_001071"
     CDS             8229..8498
                     /locus_tag="VYQ24_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1968220.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            8498..8878
                     /locus_tag="VYQ24_001072"
     CDS             8498..8878
                     /locus_tag="VYQ24_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1968221.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            8967..9194
                     /locus_tag="VYQ24_001073"
                     /pseudo
     CDS             8967..9194
                     /locus_tag="VYQ24_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9234..9959)
                     /locus_tag="VYQ24_001074"
     CDS             complement(9234..9959)
                     /locus_tag="VYQ24_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1968222.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGKILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTILKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            10121..11962
                     /gene="typA"
                     /locus_tag="VYQ24_001075"
     CDS             10121..11962
                     /gene="typA"
                     /locus_tag="VYQ24_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1968223.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            11982..12236
                     /locus_tag="VYQ24_001076"
     CDS             11982..12236
                     /locus_tag="VYQ24_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1968224.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLLLAYFSVRDITLMPVKKSKRR"
BASE COUNT         3594 a         2374 c         2818 g         3569 t
ORIGIN      
        1 aaatcaggcg gatacatggc taaaggtgaa tgggtttatg atgccaccta tcaagcttgg
       61 tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa ttactatcta
      121 aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta ttatgttggc
      181 gctgacggag tttggaagga aggtcaagca agtacagctt cttctagtaa tgatagcaat
      241 agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt ccacatgtca
      301 aaaaaaaacg tatgtataga caattaactt ctgattttga taaattttca aatgatgcag
      361 ctcaatatgc cattgatcat tagatgatta acagatactg gtaggcgaaa aaattcctta
      421 taattcattt acgcaaacct ttgcataagt gaatggattt tgatatacta gtactgtaag
      481 cattttcaat tactacacaa tgaaaaagga gaatatgatg agtcaaaaga ttattgggat
      541 tgaccttggt ggaacttcta tcaaatttgc aatcttaaca acagcaggag aaatccaagg
      601 aaaatggtca atcaagacca acattttgga tgagggaagt catatcgttg atgatatgat
      661 tgagtctatt caacatcgtt tggacttgct tggattggca gcagcggact tccaaggcat
      721 tggaatggga tcaccaggtg tggttgaccg tgacaaaggg actgttatcg gtgcctacaa
      781 cttgaactgg aaaacccttc aaccaatcaa acaaaagatt gaaaaagctt tgggcattcc
      841 atttttcatc gataatgatg ccaacgtagc agctcttggt gagcgctgga tgggtgctgg
      901 agataaccaa ccagacgttg tctttatgac actcggtact ggtgttggtg gcggtatcgt
      961 cgcagaaggc aaattgcttc acggtgttgc tggtgcagca ggtgagcttg gtcacatcac
     1021 tgttgacttt gaccagccaa tctcatgtac ttgcggtaag aaaggctgcc ttgagacagt
     1081 tgcttcagca acagggattg tcaacttgac tcgtcgctat gccgatgaat acgaaggcga
     1141 tgcagccttg aaacgcttga ttgataatgg agaagaagta actgctaaga ctgtctttga
     1201 tctcgcaaaa gaaggagacg accttgcttt gattgtttac cgtaacttct cacgttactt
     1261 gggaatcgct tgtgctaaca ttggttcaat cctaaaccca tcaacaatcg tcatcggtgg
     1321 tggtgtgtca gctgcgggag aattccttct acaaggtgtt caaaaagttt acgatgaaaa
     1381 tagtttccca caagtacgca catctactaa attagctctt gcaactctag gaaatgacgc
     1441 tggagttatc ggagcagcat cacttgtatt gcaataagat aatgagagag gaaaatgcta
     1501 acctagagtt agtaatgttc ctcttttatt ttttatgcta tactagttag gacaatgaat
     1561 gaggtgagag taaatgacaa aagcagatac gatttttaaa gagaatattg aatgcattct
     1621 aaaggaaggt gttttctcag agcaagctcg tcctaagtac aaggatggga ctgttgctaa
     1681 ttccaagtat gtaactggtg ccttttccga gtacgactta tctaaaggag aatttcctat
     1741 cacaaccttg cgtcccattg caatcaaatc cgccatcaaa gaagttctct ggatttacca
     1801 agaccagtca aatagcttag aagtgcttaa tgacaagtac aatgttcact actggaatga
     1861 ctgggaagtt ggagacacgg gaaccattgg tgagcgctat ggtgccgttg ttaagaaaca
     1921 cgacattatc aataagcttc tcaaacagtt ggaaaccaat ccttggaatc gccgcaatat
     1981 tatttcgctc tgggattacc aagctttcga agaaacagat gggctgctcc cgtgcgcctt
     2041 tcagaccatg tttgatgtcc ggcgtgttga tggggaaatc tatctggatg cgaccttgac
     2101 ccagcgctcc aatgatatgc tggtggccca ccacatcaac gctatgcagt atgtggcttt
     2161 acagatgatg attgccaaac attttggttg gaaggttggg aagttcttct atttcatcaa
     2221 caacctccat atctatgata atcaatttga acaagctcag gaattgctcc gtcgggagcc
     2281 gtcaaactgc caaccacgct tggttttaaa tgttcctgat gggactaatt tctttgatat
     2341 caaagcagaa gattttgagt tggtggatta tgaccctgtt aagccacagt tgaagtttga
     2401 cctagctatt taaaagaata gaaaaaagaa gttgagaata atcccaactt cttttgtttc
     2461 ttaacgtgat acgcggcgac gagctgcttt tttacgattt tcttcgatga aagctgcttt
     2521 ttgctcttct ggttcgatta ctttcttttt aaatgcgtat actgcacctg caacggcagc
     2581 gacagttcct gcgacacctg ttacaagacc tttagcgaat cctttagcca tgagtcttcc
     2641 tcctttatat tctcaatcag ccagcctcct caagaggtca catttttctg actgaccttt
     2701 ttgtgttata ataatagtaa cgaaaaaatg ggaatttttc aaggaaaaaa gatgaaaaca
     2761 aaaataattg tgattgttgg accgactgct gttggaaaga cggctctagc tattgaagtt
     2821 gcaaagcgct ttaatggcga agtggttagt ggagatagcc agcaagtcta tcgaggactt
     2881 gatattggga cggccaaggc tagtccagaa gagcaggcag ctgttcctca tcatttaatc
     2941 gatgttagag agataaccga gtcttactcg gcttttgatt ttgtttcaga agctaagatg
     3001 actattgagg atattcagag ccgtggcaag ctagccatta tcgccggtgg gactggactt
     3061 tatatccaga gcttgctaga aggttaccac ctaggtgggg agactcctca tgaggagatt
     3121 ttagcttatc gagctagttt ggagccatat tcagatgagg aattagccca tttggtggag
     3181 caagcaggcc ttgagattcc ccagtttaat cgtcgtcgtg ctatgcgtgc cttagaaatt
     3241 gcccattttg gtcaggattt ggaaaatcaa gagatcttgt atgaaccgct gattatctgc
     3301 ttggatgatg aacgtagtca actttatgag cgtatcaacc accgagtgga tttgatgttt
     3361 gaggctgggc ttttggatga ggccaagtgg ctgtttgacc attcccctaa tgtacaggct
     3421 gctaaaggaa ttggctataa ggaactcttt ccttatttcc gtggagagca gacctttgag
     3481 gaagctcgtg agagtcttaa acaggcgacc cgtcgttttg ccaagcgtca gctgacctgg
     3541 ttccgtaatc gcatgcaggt caccttttat cagattggag aatctggtgt acaagaccgc
     3601 attttaagcc agatagagga gtttttagat gattgaaacg gagaaaaaag aggagcgagt
     3661 cctgctgatt ggtgtggaat tgcagggtat ggacagtttt gacctctcca tggaagaatt
     3721 ggctagttta gcgaaaacgg caggggcagt cgttgtagat agctacagac aaaaacgtga
     3781 aaaatatgat tccaagacct tcgtcggctc tggtaagttg gaagagattg cgcttatggt
     3841 ggatgcagaa gaaatcacta ctgtcatcgt caacaatcgt ctgaccccaa ggcagaatgt
     3901 caatctagag gaagttctcg gtgttaaggt cattgaccgt atgcagttga ttttggatat
     3961 ctttgccatg cgggctcgaa gccatgaagg gaagctccaa gtccacctag cccaactcaa
     4021 atacctcttg cctcgcttgg ttggtcaggg gattatgctc agccgtcagg cagggggaat
     4081 tggttcccgt ggtcctggtg aaagccaact ggagctgaac cgtcgtagcg ttcgcaatca
     4141 aatcacggat atcgagcgcc agcttaaggt ggttgagaaa aatcgtgcga ctgtcagaga
     4201 aaaacgtttg gagtctagca cttttaagat tggtttgatt ggttatacca atgctgggaa
     4261 atcaactatc atgaacatct tgaccagtaa gacccagtat gaagcagatg agctctttgc
     4321 gactctggat gcgacaacca agagtattca tctgggaggc aacctccaag taactttgac
     4381 agataccgtt ggctttatcc aagatttgcc gacagagttg gtgtccagtt tcaagtcaac
     4441 cttggaagaa agcaagcatg tggaccttct ggttcatgtt atcgatgcta gcaatcctta
     4501 ccacgaggag catgaaaaaa cggttctctc catcatgaaa gacctggaca tggaagatat
     4561 tcctcacttg acgctttata ataaagcgga tttggtggag gatttcacgc ctacccaaac
     4621 gccatatacc ctcatttctg ccaagtctga ggacagtcgt gaaaacttgc aagcattatt
     4681 gctagataag attaaggaaa tttttgaagc atttaccctg cgagtgcctt tttcaaagtc
     4741 ctacaagatt catgatttag agagtgttgc aattctggaa gaacgtgatt atcaggaaga
     4801 cggcgaagtg attacaggct acatttccga gaaaaataaa tggaggttag aagaatttta
     4861 tgactgatat taaaactttg gctctaaagt atggaggtta tacaagtctg gacaaggtct
     4921 atctggatca gcttctagct ggtaaaacag atcaggagca gttaacactt attacacctc
     4981 cgccaagtgt tgttaatgcc tactttgcag aactctatca gaaaaagagt cctcaggctg
     5041 cgacggatta ttttgcagaa ctcagtcagg aactgaatct ttacaatact gagccaagtt
     5101 tcaccctaga aaataagcct tttattcggc ttaatctgtc tggtaaatcc tttggttttt
     5161 gttatgagag tgacggcctt ggtcggattt tctttgaaaa tgaggagaaa atctcggatg
     5221 acttgctctt tgaaattgcg caaattttcc cccatcaatt agtctttgaa gagtctggta
     5281 agatttacat gaagcctgtc gaggacgagg aagttatcag cctagaaagt ctcacagctt
     5341 tgactgattt ggaaagattg gctgatggtc gaaaacgtct caaaggctac agccaagagg
     5401 atttattgca agaagctgct gctttttctg gcaaacgcta tttccgatcg gaaaaccgca
     5461 cagccatgtt atatattgat taattagaaa gtatcgaatg gatattcaat ttttaggaac
     5521 gggggctggt cagccctcta aagcccgcaa cgtttcaagt ctcgccctga aacttttgga
     5581 tgagattaac gaagtttggc tctttgactg tggagaaggt acgcaaaatc gcattctgga
     5641 aaccacaatt cgaccacgta aggtcagcaa aatctttatt acccatctgc atggagacca
     5701 catttttggt ttgccaggtt tcctttctag ccgtgccttt caggccaatg aagagcagac
     5761 agatttggaa atctacggac ctcaaggaat caagtcattt gtcttaacca gccttcgtgt
     5821 gtcaggttct cgtctgccct accgcattca tttccatgag tttgaccaag attctctggg
     5881 taaaattctt gaaaccgata aattcactgt gtatgcagag gagctggacc acactatttt
     5941 ctgtgttggc tatcgtgtca tgcaaaagga tctagaaggg acgctggatg ctgaaaaact
     6001 caaggctgct ggtgttccgt tcggcccgct ttttggtaaa atcaaaaacg gccaggatct
     6061 tgttttggaa gacggaactg aaatcaaggc agcagactat atctcagcgc cacgtccagg
     6121 taagattatc actattttag gagacactcg aaaaacgggt gccagtgtgc gtctggctgt
     6181 caatgcagat gtcctagttc atgagtccac ttatggcaag ggtgatgaaa aaattgctcg
     6241 taaccatggt cactcaacta atatgcaagc tgcacaagta gcggtagaag caggtgccaa
     6301 acgcctccta ctcaaccata tcagtgcccg tttcctctca aaagatatta gcaaactcaa
     6361 gaaagacgct gccacaattt ttgaaaatgt ccatgtggtc aaagacttgg aagaagtgga
     6421 aatctagcag tcacagaaag gataagtatg cctactattc tcattaccgg agctagcggt
     6481 ggtctagctc aagaaatggt caaactcctg cccaatgacc aactcatctt gcttggtaga
     6541 aataaggaaa aattagccca actctacgga aattattccc atgcagaatt gattgaaatt
     6601 gatattaccg acgattcagc cctagaagct ctggtaactg atctttatct ccgctatggc
     6661 aagattgatg tcttgattaa caacgctggt tacgggattt ttgagggatt tgaccagatt
     6721 gctgataaag atattcacca gatgtttgag gtcaatacct ttgccctgat gaatctgtct
     6781 cgtcaccttg cggctcgtat gaaggaaagc agcaaagggc atatcataaa catcgtcagc
     6841 atggcaggtc taatagctac tggcaagtct agtctttact cagcgaccaa gtttgcggct
     6901 attggttttt caaatgctct gcgactcgaa cttatgccct atggagtcta tgtgacaaca
     6961 gtcaatccag gtccaatccg aacaggattt ttcgaccaag ccgacccaga tggaacttat
     7021 cttaaatcgg ttgaccgctt cctgctagaa ccagatgcag tggctaaaaa gattgtcaag
     7081 attataggaa aaaacaaacg agaactcaat ctcccgattt tgttgaacct agctcataag
     7141 ttttataccc tctttcctaa gctagctgat aagttggcag gggaaacttt taattataag
     7201 taaaaagaac caatgtgcag gttgttgcta gcctacatat tggttcttat catttctcaa
     7261 ctatcaaact gaacttcttc taggagatag tctataaagc gttcgcccat cttagatagg
     7321 ctggttttct catgctggat atagaccagc tcgatcgggt catcaatatc cagtgggata
     7381 gaaacgatat tgtctccgtt taggttgctg ttcaaaatcc ctgtcgcaat ggtataacca
     7441 tccaaaccaa tcaagagatt aaagagggtg gcacggtcac tgaccacaat ggatttcttg
     7501 tggtgttctt gagaaagaat ctcttctgaa aagtagaagg agttgtgcgt cccttggtca
     7561 tagctgaggt aagggaaatt ctccaaatca gacagtttca ccttgtcttt ctttgccaga
     7621 gggttggtct tgctgacaaa gatatgtggt tgcgctgtga agagatggtg ggctagcagg
     7681 tgattgtcat ccagcatctt ggttaaaaca tcacggttgt aactgtttaa gaagaggacc
     7741 ccgacctcac tgcggaagtt cttgacgtcg tcgataatct cccaagtccg agtttcacga
     7801 aggaagagtt cgtatttctc catatcgctt ttcttgagca aagagacaaa ggcattgacc
     7861 acaaaggcat agtgttgaga cgaaacgcta aatagttcgc ggtgggcgac aggattttta
     7921 tagcgttcct ccagaagctg ggtctgctcg acaacctgac gggcataaga gagaaactcc
     7981 atcccatcac gggtcaaggt gattcccttg ggattgcgga taaagatctc aatgcccatt
     8041 tcattttcca aatctcgcac tgcattggag agacttggct gagtgataaa gagttgcttg
     8101 gctgcctcat tcatggagcc agtttcgacg attttgataa tataatgtaa ttgttgaatt
     8161 ctcatgtttt tattgtacca tacttgcctt agaatcgcta aagaagatag gaaaataagg
     8221 agcattgtgt gagtctcttc ttgaaaaaaa cacaaaattt tgctagaatg agtcttatga
     8281 aaacattcta tgatgtgcag caattcctca aacgatttgg tattattgtt tacatgggaa
     8341 aacgcttata tgatattgaa ctgatgaagt tggaactatc tcggatttac gatgcagggt
     8401 tgatggataa attagactat ctagaggcag aagcagttct tcgcagagag cacaaggtag
     8461 aattggatta tattgagaaa aatggagaaa agaactaatg gttacttgga ttttgtgggc
     8521 acttatacta gcaatgttgg cgtggatggg ctttaactat cttcgtattc gccgtgcggc
     8581 taaaattgtg gacaatgagg agtttgaagc cttgattcgt acgggtcaat tgattgattt
     8641 gcgcgaccca gcagaattcc acagaaaaca tatccttggt gcacgcaata ttccttcaag
     8701 tcagttgaaa actagtcttg cagcccttcg taaagataaa cctgtccttc tctacgagaa
     8761 ccaacgtgcg caacgagtta caaatgcagc tctttacttg aaaaaacaag gtttttctga
     8821 gatttatatc ctttcttatg gcttggattc ttggaaaggg aaagtgaaga ctagctaacc
     8881 ttatttccaa attagtcgaa tgataggatg aagctattgt tttatagtat aattaatttg
     8941 tataaaattt aaggagttct tagaacataa aagaaaagaa gaaaaatcca ctttttgtgg
     9001 gtatcttgtc gattattctt ggtttgttat ttccaattgt aggcctgatt ttgggtatca
     9061 taggattggt cttggctatt tcataccaaa aagagtcata attagactat aaaatagaaa
     9121 agattcttaa tatcttagga attgtgattt ctgtagttaa ctggattgta gcaatcgcct
     9181 taatttttcg gtaaataaaa gaaaaaagct ttagttttaa agcttttttt gttttatcga
     9241 ctcatctcta aatagttttt aatgatttgt aactcttttt ggttcaaagg gcggtagtta
     9301 ctttctgcta aatctgggtt caatgtgaag tccccaaatt ggattctctt gaggatagtc
     9361 accttaacac caaccgagag gaacattttc ttgatttgat gaaattttcc ttctgaaatg
     9421 gtgatagagg cttgactgat ggagggactt gcagatagaa tctctagttt tgcgggttta
     9481 cagacagtac catctaaaaa gacaattccc ttttgaaagg cttggatatg gtcaggtgtt
     9541 agaagtccat taacctcaac ttgataagtc ttatcgacat gatattgggg atggaggagt
     9601 tggaagccca agggaccgtt atcggtcaag aggaggagtc ccgttgtatc tcggtccagt
     9661 cggccaacgg catagagctt gtcagactgg atgtcagatg gaagcaggtc catgacggtc
     9721 ggaagttcct tgtctttgtt ggctgtaacg acaccagcag gtttatgaag cataagatag
     9781 gtgtgttcat agccttgaat gattcgatcc tgaaaaagga gttcttgtag tcctgtatcg
     9841 atattttgag ctagggagcg ggctgggcaa ccatcgacta gaattttccc tctgaggagt
     9901 gcttgcttca tggcctttcg gctgattttt tcttgggcta ataaattatc taaacgcata
     9961 ctgtgcctat cttatcataa gtcgcttgct ttgaaaaggc ttgacgtgaa aagaaaagcg
    10021 gagtgaaaac atttacactt gcttgagtta tgttatttat ttgaaattat ggtataatcg
    10081 ttcagttaga aaataaattt tgaatattat agaggaaatc atgacaaaat taagagaaga
    10141 tatccgtaac attgcgatta tcgcccacgt tgaccacggt aaaacaaccc tggttgacga
    10201 attattgaaa caatcagaaa cgcttgatgc acgtactgaa ttggcagagc gtgctatgga
    10261 ctcaaacgat atcgaaaaag agcgtggaat caccatcctt gctaaaaata ctgccgttgc
    10321 ttacaacgga actcgtatca acattatgga cacaccagga cacgcggact tcggtggaga
    10381 agttgagcgt atcatgaaaa tggttgacgg tgttgtcttg gtcgtagatg cctatgaagg
    10441 aaccatgcca caaactcgtt tcgtattgaa aaaagccttg gaacaagacc ttgtcccaat
    10501 cgtggttgtt aacaaaatcg ataagccatc agctcgtcca gcagaagtag tggatgaagt
    10561 cttggaactt ttcatcgagc ttggtgcaga tgacgaccag cttgatttcc cagtggttta
    10621 tgcttcagcg atcaacggaa cttcttcatt gtcagatgat ccagctgacc aagaagcgac
    10681 tatggcacca atctttgaca cgattatcga ccatatccca gctccagtag ataactcaga
    10741 tgagcctttg cagttccaag tgtcactttt ggactacaat gacttcgttg gacgtatcgg
    10801 tatcggtcgt gtcttccgtg gtacagttaa ggttggggac caagttaccc tttctaaact
    10861 tgacggtaca actaaaaact tccgtgttac aaaactcttc ggtttctttg gtttggaacg
    10921 tcgtgaaatc caagaagcca aagcgggtga cttgattgcc gtttcaggta tggaagacat
    10981 ctttgtcggt gaaaccatca ctccgacaga tgcagtagaa gctcttccaa tcctacacat
    11041 cgatgagcca actcttcaaa tgactttctt ggtcaacaac tcaccatttg ctggtaaaga
    11101 aggtaaatgg gtaacttctc gtaaggtgga agaacgcttg caggcagaat tgcaaacaga
    11161 cgtttccctt cgtgttgacc caactgattc accagataaa tggactgttt caggacgtgg
    11221 agaattgcac ttgtcaatcc ttatcgaaac aatgcgtcgt gagggctatg aacttcaagt
    11281 atctcgtcca gaagttatcg taaaagaaat cgacggtgtt aaatgtgaac catttgaacg
    11341 tgtacaaatc gacactccag aagaatacca agggtctgtt atccaaagcc tttctgaacg
    11401 taaaggtgaa atgttggata tgatttcaac tggtaatggt caaactcgtt tggtcttcct
    11461 tgttccagcg cgtggtttga ttggatactc aactgagttc ttgtcaatga ctcgtggtta
    11521 cggtatcatg aaccatacct tcgaccaata cttgccattg attccagggg aaattggtgg
    11581 acgtcaccgt ggtgcccttg tttctatcga tgctggtaag gctacaactt actcaatcat
    11641 gtctatcgaa gaacgtggta cgatctttgt caacccaggt actgaggttt atgaaggaat
    11701 gatcatcggt gaaaactctc gtgaaaatga cttgacagtt aacatcacga aggcaaaaca
    11761 aatgaccaac gttcgttcag ctactaagga ccaaacagct gttatcaaga cgcctcgtat
    11821 cttgacactt gaagagtctc ttgagttctt gaacgacgat gagtacatgg aagtaacgcc
    11881 tgagtctatc cgtttgcgta aacaaatcct taacaaagca gagcgtgaga aagctaacaa
    11941 gaagaaaaaa tcagctgaat aagagctaga aagagagaaa gatggtctat ttagtcctag
    12001 gaattttact gctcctactc tatgtatttg cgacaccaga aagcattaaa gggacagtca
    12061 atatcgtcgc tatggtatgt attttagtgg cactcttgat tttattggtt ctatcttttc
    12121 tgaaaatttt tcaattacca acagaaatat tcctagcaat agccatgttg ctcctagctt
    12181 actttagtgt tagagacatt acactcatgc cagtcaaaaa aagtaaaaga agataaaaaa
    12241 gagagttttg gctctctttt ttgctaggaa ttttacaaga ttgcaattta tttttgagtt
    12301 atcaccatct cttttgtttg gataatatga atgggaaaaa cttgaataaa attcg
//
LOCUS       DAVMEI010000052        12180 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVMEI010000052 DAVMEI010000000
VERSION     DAVMEI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.052"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(59..1876)
                     /locus_tag="VYQ24_001077"
     CDS             complement(59..1876)
                     /locus_tag="VYQ24_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1968225.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILVAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(1974..3116)
                     /gene="tgt"
                     /locus_tag="VYQ24_001078"
     CDS             complement(1974..3116)
                     /gene="tgt"
                     /locus_tag="VYQ24_001078"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1968226.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            3246..4103
                     /locus_tag="VYQ24_001079"
     CDS             3246..4103
                     /locus_tag="VYQ24_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1968227.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(4131..4775)
                     /gene="pcp"
                     /locus_tag="VYQ24_001080"
     CDS             complement(4131..4775)
                     /gene="pcp"
                     /locus_tag="VYQ24_001080"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1968228.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVTINQDDACISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKSPYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(4882..5241)
                     /locus_tag="VYQ24_001081"
     CDS             complement(4882..5241)
                     /locus_tag="VYQ24_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1968229.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            complement(5252..5677)
                     /locus_tag="VYQ24_001082"
     CDS             complement(5252..5677)
                     /locus_tag="VYQ24_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1968230.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIRVKEEIESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(6000..7112)
                     /locus_tag="VYQ24_001083"
     CDS             complement(6000..7112)
                     /locus_tag="VYQ24_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1968231.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEVTKTVIASEEVAPST
                     GTSVPEEQTTETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTSSEAKEVASSN
                     GATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAAFKEEIANLFG
                     ITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGISYIIWKQRFY
                     APFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(7278..7898)
                     /locus_tag="VYQ24_001084"
     CDS             complement(7278..7898)
                     /locus_tag="VYQ24_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1968232.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(7902..9182)
                     /locus_tag="VYQ24_001085"
     CDS             complement(7902..9182)
                     /locus_tag="VYQ24_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1968233.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(9243..10727)
                     /locus_tag="VYQ24_001086"
     CDS             complement(9243..10727)
                     /locus_tag="VYQ24_001086"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1968234.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFANVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(10803..12080)
                     /locus_tag="VYQ24_001087"
     CDS             complement(10803..12080)
                     /locus_tag="VYQ24_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1968235.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
BASE COUNT         3711 a         2797 c         2138 g         3534 t
ORIGIN      
        1 ttttggagat attttaaaag agctggattt ctccaactct tctctttttt aatacaagtt
       61 attttgattt aaccggcttg tcttgagctg tctgcaaagc tgtggcaatc gtatctgcat
      121 acaattttgc tcctgcttcg atagtgctac tctcactccc gaaatgaacc tggtctgttc
      181 cagcccaaat ttctggatgc tctttcgcaa cttgattcca atctgctatc gtaatgtaag
      241 gtgtcttctc tgccaattct ctcatatagg cagcagcctt ctcaacgatg gcataggtct
      301 cttttgtctt atctccctca taaggagtca ccaaaatcat atggtgtccc ttaggaagat
      361 ttttcacgat actgtcccag tcatccttgt aattctcagg attatttacc ccagtcgcaa
      421 tgaccaccgt cttaggtaaa aatttattct ggctattatt tagcatgatt tcatttgcgg
      481 tcttggttgt tacgctgacc tgcgcgttaa tctgtgctcc aggaagagct gtctgtagtg
      541 ctgtatttgc ccttaaagcc actgagtcac caattaacat agtgccatca gcaattccca
      601 aactgtttgc atctgcccgt tctgccatca ccttggtctg gccaatattt gttgcagctt
      661 gcttcaagcc attgacagtc aagtctgtct caaacgctcc cacttgtggt gccaacaagg
      721 tcaccgtgca gacaatgatg gtcaagattc ctgtacctgc tacaagaatt gcgtgaatat
      781 aaggcagggg acgaagagtt tgtaaaatag gtgtgttctt gcctgcaatc caaggttcca
      841 atacatagaa tgacagactg gcaaagccat aagaacaaat cagagtcagt aatacagcaa
      901 gaagatttga tgtcaactgt gagaaaatga tatagaaagg ccaatggaaa agataaaccg
      961 catagctagt atccgctaaa aagctgataa tcttcggctc ctgtatatga tgtgtctttt
     1021 catgtaagac acgcgccgcc agaatcatgg caagagctgc aagactggca agcaagaagc
     1081 cgataagata ggcaaaaaga taagtgaatt tgacaaagaa agtcaaaaga actaggaaac
     1141 caaagcctcc tccaaaaact accaaagtct ttcgtaaatc ccagatttta tccaactgct
     1201 tgacgaggga agtcgtctga cgaacaccta caatagttgc taacatactt cccaaaaaga
     1261 atggatagac atgagttaaa ctggagaaat aaacagagga ataagaggtc actagaaaac
     1321 taccaataaa catggagaag aaactgatca agaaggcagc agcagataag agaaagacca
     1381 tccccttcaa ctgaccattt gatttagctt gtttggataa gaaccaaact gccaatcccc
     1441 aaagaatata gtagtgaacc tcaactgcca aactccaatt atgaacaaac aaatgaggaa
     1501 tgaactgaga ttcataactc ccacctgtta ggagttcata gaagttggtc ataaagccta
     1561 agacgcccgc aatctggcca ccaattccag caacatagtc ttggcgaacc aagaaagtaa
     1621 aaggcatggt caccaagacc atcaaaacca caggtggcac aatccgataa aagcgtcttc
     1681 taaaaaatcc tatcaaatca atctcattgt tcttagaaaa ttcttcaatg agtagagctg
     1741 taatcaggaa acctgaaaat gtgaaaaaga catctacccc gaaaaatcct ccaggaaaga
     1801 tcgtctgaaa gaagtgatac aagagtacca aaagtaaacc tataatccta atcaaggaaa
     1861 accatttaat gcgcatacga gtttattctt cctttcattg tacactttat tctatcaaaa
     1921 aaagaagaaa aatcctaaga gaaaacttag gattttttta tatcaattcc attttagaaa
     1981 ttacgtcctg acttattata gccatatttt tccacaaaat actcacggaa ttccaagaga
     2041 ttgtcatcca tgatggcttg tcgcacttgc ttcatcaggt taagcaagaa gtaaagattg
     2101 tggtagctag tcaagcggat accaaaggtt tcatcagcct tgagcaggtg acgaaggtaa
     2161 gcgcgtgtat agttattaca tgtgtagcag tcacactcag gatccagtgg cgtaaagtcc
     2221 tcagcaaact gggcattttt cacaaccaaa cgtccttgac tggtcataca agtcccgtta
     2281 cgagcaattc gagtcggtaa gacacagtca aacatatcca ccccacgaat gaccccatcg
     2341 atcaagctat ctggcgctcc cacacccatc agataacgag gtttattttc aggtagcagt
     2401 tgagttgtaa agtccaagac cgcattcatc tcttcatggg tttctcccac tgccaaacca
     2461 ccgatagagt agcctgagaa atccatgctg acaagatcat gagctgattg gcggcgaagg
     2521 tcttcaaatc ctgcaccttg cacaattcca aacaaacctt ggtcatgtgg acgacggtga
     2581 gccttcaaac cacgctcagc ccaacggctg gtacgctcga tcgatttctt aacgtagtca
     2641 taaggttgat aaaactgagg acattcatca aaggacatca tgatgtctga acccagatta
     2701 ttctgaatag agatggcttt ttctggggat aggaacatct tagaaccatt tagatgattt
     2761 ttaaaggtta ctccttcttc tgtgatatta cggctatctg ctaaagaata aacctgaaaa
     2821 ccaccactat ctgtcaagat aggctggtcc caattcatga acttgtggag accaccagcg
     2881 cgtgcaatga gttcatctcc agggcgaagc cagagatgat aggtgtttga taggataatt
     2941 cccgaaccca tctccttcaa ttcttcaggt gactgagttt tgacagtggc ttgtgtccca
     3001 actggcataa acataggtgt cggaaaggta ccgtggggag tgatgatttc tcccagacga
     3061 gctcctgtgt gtttttcttt cttaatcaaa cgatatttga ttggtgaatc tgacattttt
     3121 tacctccgaa gctgggaaag acagtcccag ttcatacagt gtgcccaagg acacactgta
     3181 tgattttatc aaaaaaagta ggaactgtca cgaactatgt ataagaacta tggtataata
     3241 agagaatgaa ctatccaaaa attgatttaa aaacaattcg tcaggagtca aaacattttc
     3301 aggctgacac cccccgtctc tttctcctct atatccttcc cagtatgctg gtcatcttat
     3361 ctggttttct taatccctta tctcggatcc acgggactgt tttagaacag ccatttttca
     3421 gcatacttgg ccaaatactt caaacctacc tctttcctct actagtctcc tttatcggga
     3481 ctattctttt gaccagttcc gtctatgcaa cactgacact catgaaagat tctaaaacgg
     3541 agccatctgt aaaaaacagc ctagctctct ttgatgagga gcgcttttct caaacctttt
     3601 tgaccctact tctcaaacgt ttctatctct ttttatggag tatccctaat ttacttggga
     3661 tttacctcct cttttatagt agctttttag ccaaaaaatt cgtcacccta catcctgagt
     3721 ttccaaatct agatctctca tcagttgaaa ccgagcgttt cctcatggtc tttggactct
     3781 actttcttgc aagtctcatc ttgatcattg taggaaatat cctctatatt ccacagtatt
     3841 atgcctattc acaggtggaa tttcttctct gttatagtct agacctagga caggtcccac
     3901 caagacgaat cctaaaaact agccgttcct ttatgaaggg ctacaagttc cagcactttg
     3961 tcctagattt acagcttctt ccttggtact tcctcaattg gattaccttt ggcattgcta
     4021 gtttttcact cctaccttat atccaatgca ctaaaattat gttttaccga gcagtattgg
     4081 cccgaaaacg tccaaaagct tgaaggtttt tcccctcaag cttttttcta tcaatgagtt
     4141 tctccgccta ccaacttgag ttcctgatct ccatgttcta ttatagcgcc gattgctgct
     4201 tctatccctc gccgaatatc cactaaactc atagctggag tagtcggtct gttcaccacc
     4261 tgttccatca tataaggaat atgcataaaa cctgccttaa catatggaga tttcttttct
     4321 accaaataga gagcctgata catcaaatgg ctgcagacaa aagtccctgc cgtattggaa
     4381 acagaggccg gtaagccctc tttttttata gcttgaacca tcgctttaat cggcaaacta
     4441 ctaaagtagg ccgaagcacc atctgggcga atgggacggt caatcggttg attatcttcg
     4501 ttatcagaaa tgcatgcatc gtcttgatta atggtcactc gttcaggtgt caaactagtt
     4561 cttccaccag cttgcccaat acaaaggaca aagtcaggtt gataacgatt catctcttct
     4621 tccaatactt gagcagattt gtgaaaaact gtcggcacct ctagccaacg gacctcagca
     4681 ccatggattt cagctggtaa acctttaatg gcctccaaag ctggattgcc cttttcccct
     4741 ccaaagggct caaaacctgt cactaatact ttcatttcta actcctcaaa accaattcaa
     4801 tgagactttt ttcttaaaaa caagtataac atatttccct tattctggat taaaaaggat
     4861 tagcgagatt agaatcgacc ttcaagtgta tttaaagagt aaaatactaa tcaaggccaa
     4921 aatagctgat ccaccttgtt tcaaaataat ttttttatcc gctgttaaag agccgtaagt
     4981 cgcagcacca atcacaaata agacaaaaat agtcacaatt tctaaattct gtgagaaata
     5041 aatgacatac aagagaaaga ctcctagcag agccttataa attccttgat ttttgaacaa
     5101 tgaacttact gacggatgag ccaattcttc cttttccata ttaaatacac gactagtcgc
     5161 atctgattgc gtggcaatac tttccaaata aaaaatgtaa aaatgctcca aagcaacgat
     5221 cgttgttaaa ataattgaca tctatttctc cttaaatttc tatattttca atacccaaaa
     5281 ccaatttatt taataaacgg ctgagttctg ttctctcaaa ctctgttaag atactttcta
     5341 tctcttcctt aaccctgata tgttgcagag ggggattcac cactaactgc tccttggcat
     5401 acttcgcagc ctctaccaac acttcccgct gattttcagg attacgatga cgctccacca
     5461 aaccttccgt ttccaaaatt ttgaaatgcc gtgtcaaagc agcctgatca attttcaaac
     5521 gctcctgaac cgccatttgg ttacaaggag aatgctccag caaaaacagt aaaatctgat
     5581 accgtgtcaa actaatccca agcctttttt caaacaattg cgtactcgct tgctcagaca
     5641 agcggagttg atacagtaaa tcttgaatct ctagcatttc ttcccctctc tttttattga
     5701 tttatcaata tttgactaat taattgtgat ataaagtaaa atagattgca agaatgttgc
     5761 ttaactcatt acaagatgat aactttccat tttccattca aattgatact caatgaaaat
     5821 caaaaagcaa actaggaagc tagtcgcagg ttgctcaaag cacagctttg aagtttcaga
     5881 taaaactgac gtggtttgaa gagattttcg aagagtatga caaagaaaaa acgaacgaga
     5941 atcgctttcc atcacgaaag ctagatctca ttcgtcaaaa tgatgttatc aagtcgggtt
     6001 tatccattca ttgaaacgtg aacgtgatca tagtgatttt ctgtcacact accacggtct
     6061 ggcattgggt tccaagtgtt agctggccca tatttgctat cgaatggagc atagaaacgt
     6121 tgtttccaga tgatgtaact aatgccacgg ctggccatat tttgaatagc atattccgca
     6181 atcttatccc ctaattctga acgttctggt accataaagt cgatagccaa accttttccg
     6241 tgatctccac tgtctcctgg acgataacca ctaaaggatg taatgccaaa caagttagca
     6301 atttcttctt taaaggcagc tgtttgtggt tgaagacctg cattttcaga ttttgctact
     6361 gcaagtccag cataatcggg cgcagctgga gcctcgtaag ttgttgaaat tattttcgtc
     6421 tcttctggtt gataagtaga aactgctgct gtagctccat ttgatgatgc tacttctttt
     6481 gcttctgaac ttgttgtagt tacttccact gctgatgcag cttgagggct tgcttgtgtt
     6541 tcctgcttct cagctggagt cgtttcctga ggagttgctt cttcaactgg gcgagttgtt
     6601 tcggtcgttt gctcctctgg gacagaagtg cccgtagatg gtgccacttc ttcagaagca
     6661 atcactgtct ttgtaacttc tgcaattggt tgagaaaggt ctgcaacctg aacagtttga
     6721 tcatcaacgg tcacttgatt agtggtcaaa tctgctgtcg cagttgtcac ttcttcacta
     6781 gagtctgctt gaggtgtttg gatttcaact tctgttactt cttctgcttc attgacagtc
     6841 gttgtcaaaa cagtttctgg gaaaatcaag tccatattag tgattttgtt cagattcgca
     6901 agcactgtta catctacacc caaggcttct gcaatggtgc tcaaagtatc accatactgt
     6961 acggtataac ttgttttgtt gtccgtttta gtcaaatcgt tttggatttg ctcaacacta
     7021 cgtgcagtcc aaagaacttc ttctgcttga gttgccaata ctggggcaaa tgacaaggct
     7081 actgttgacg ctaataacat tcttttcttc attctttcaa attcctttca aatgagtacc
     7141 tgtctatcat aacacaaaaa atgtagaaaa aaagccctct cgggcctatt taacagaact
     7201 gtctattcct tgtaacaaac taggttactt gaaatagttt gttaggtctc tgtcacaaaa
     7261 ctgacacaat ctttttatca cttagtctca aaaatacggg aaatatctgc taacgcttga
     7321 atcctgtgat tcccttcata agtagactct aaaaagttga tgctttgaat cccactattc
     7381 tgggcaaatt ccacatccag agtccgatcc cctatataat aagtattatc agaattcaac
     7441 tgatacttat ctagcagata ggtagccgct tctggacttg gcttccgcac aaagccactc
     7501 tgactggtta aaatctctgt aaaataggat tccaccccca agtccttgag aatggtaaaa
     7561 gcgttgttcc ccttatgagt atatataaac tgctgaattc ctgattcgtc tgcccaagct
     7621 agcacctcac gcgcacctgg catcaaaact acctgagcat tcttctcagc cagactctgg
     7681 gcacgcacct gatttagcac ctcaacatcc agatttctat cttctgccac ccgcacaagc
     7741 aaatcttgca ccgaatactt gaagataaac tctctcacct tctccttatc ataaggaata
     7801 gaaaactgag caaaagtctc ctcaatccct gataaaatcg cttcgtaaga gtccaataaa
     7861 gtcccgtcta aatcccaaat aaaagctgtt ttttgcattt cctatccttt caagctcata
     7921 tagcgctggt aacgatagcg tagaaataac cagcgaaaac cattatccaa gagagaccct
     7981 gcccaaatac caggcaagcc ccaaccaagc acaatcccca tcagatatcc tgtcccaatg
     8041 cggatacacc acattcctat acttgtcgca taaaaaggga ggcgtgcatt tcctaatccc
     8101 tgccagactg ccgtatagat gactgttcct gtcgtcatag gggtcccaag tagtgaaaac
     8161 agtgtcacta gaacactagc ctccaccgct agagaatcag tcgtatagag atgagttaat
     8221 ggtacaccca agacatatat actaaaggac aggggcaaca tgaggaacag agaaagccaa
     8281 aaggtttgtt tactcaaact agcaactctt ttccaatcat cctctccaac tgctcgggcc
     8341 aacagcatga ccgttgccgt agcgacgcca aaggcaggca tatagttaaa ctgggtcaag
     8401 acttctccga ttgcattccc agcaactgcc tccgtcccaa aagaaacgac caaggcaatg
     8461 atcactacat ctccagccct catcataagt cgctctccag ctgctggtaa agccaaggtc
     8521 aacagttcct tatctaaacc aaaagttggc ttcccataag gcagttttaa ttgtgaccac
     8581 aaaatcacaa gaccaaccaa acgagacaca attgtccccc aagcaacacc agctatcccc
     8641 atatccagaa caaaaatagc tagacttgaa aaaagaatat tcaaggcatt ggataaaaaa
     8701 ctaacataga gaggcagacg tggattatgc gttgcacgaa tcaaggctcc tagactagtc
     8761 attaaaccta agagaacaat cgatccgcct accaaagata gatacagtcc accactctca
     8821 gctacatccc tctccgtccc caaaagtcct atcatctctt tcccagcgaa gatggacaaa
     8881 aatcctaaaa ggaaacttaa tagtaaggta atcttcaacg cctcagtcac atgataggcc
     8941 aacttcgact ggtctttctg ccctatgctt tttgaaataa cactggaaat agcagctccc
     9001 agagcgatga aaatcgcctg ataaatggtg ataatattac cagctactga aacccctgaa
     9061 atagctatca atcctaaatg agcaaccaaa taactgtcca ccattcccat tagcatctgc
     9121 aaaaagtttt cacccatagc tggcaatgca atattaagaa tgtctttatt tttcttaaac
     9181 aatctctcct cctgatgaaa agaaactcag ttggtttccc aaccgagttt gctccctctg
     9241 tcttaaagtc ctaaataagc ctcaaccgct gcttgcatgt cagcagctgc cactgttgtc
     9301 ttgtgacgaa ttggagctat ttcaagccca tcaactgctg gtggcactgc aacgcctgag
     9361 atttcatgta attgagccaa ggcttcaaag tctgttaaac ctgcttttcc agttacagct
     9421 tctactgcaa ctactgggaa cttgtatgga ctagctgttg aagcaatcac tgtcttagtt
     9481 acatctccag tggccgattg gtatttttta taaactgctg aagcaacagc tgtatgaggg
     9541 tcctcgatat aagaatctaa ctcacaaaca cgcttgatct ctgctgccgt ttcttcctca
     9601 gtcgcatatt cagctgcaaa gaggtccaaa atctctgcat caaagtctgt caacttatat
     9661 tgtccttgcg tgttcaaggc attcataagt tcagttgtct tttcagcatt atttcccaaa
     9721 agatggaaaa tcaagcgctc caagtttgaa gataccaaga tatccataga tgggctggtt
     9781 gttaccttaa actcacgttt tttgtcatag acacgtgttt taaagaagtc tgtcaaaaca
     9841 ttgttgtcat ttgaagcaca gattaattta ccaactggca aaccaatttg tttggcataa
     9901 aaggcagcca agatatttcc aaagtttcct gttggtactg tgaagttaac cttttcacca
     9961 gctacaattt caccagtctt aaccaattga gcgtaagcat aaacataata aacaatttgt
    10021 ggcaccagac gaccaatgtt catagagtta gctgatgaaa attgcaactt gttggtagtc
    10081 aatttttcac gaagagccac gtcgttaaac atgtgcttca catttgtttg cgcatcgtca
    10141 aagttaccat caatagcaat aacatgagta ttgtcgccag tctgagtggt catttgtaac
    10201 tcttgaatct tgctgacacc atcctttgga taaaagacga taatctcagt accaggcaca
    10261 ttcgcaaacc ccgccatagc agctttcccc gtgtcaccag atgtcgctgt caagataaca
    10321 atcttgttct ccaaaccatg tttcttagca gcagtcgtca taaagtatgg caaaatagac
    10381 aaggccatat ccttaaaggc aatcgttgaa ccatggaaaa gttccaaatt gtattgccca
    10441 tctaatttca ctaatggtgc aatagctgga gtatcaaatt tgctatcgta ggcattgttg
    10501 atacagtagt ccaactcctc aactgtaaag tcatctaaaa atgctgacaa aactagctta
    10561 gcaacttcct ggtaagaagc atctttcaat ttgtcaaagt tcaaatctac ctttggataa
    10621 gtatccggtg taaacaaacc gccgtccgtc gccaaacctt gcaaaattgc ttggctggca
    10681 gttactgtat tgttggcatc acgcgttgat tgataaacta atgtcattac tctctatcct
    10741 ttatctatag tctcgtccat tatatcatga tttttcagaa aattctccct ttataagaga
    10801 aattataggc tcaattcttc tctcaaaggc tgtaaatagg tcatttcttc cttatttctc
    10861 accactagtc cctcctcagc tagtaggagt aagtctttgg aaaattcaat aatcgtagtt
    10921 tcttcctcat ctgtaagatt tttcttagaa aattgtctcc ttaaagactt gtaatcataa
    10981 ccaaatactt taaagaaagg tgctgtttct aagtaagctt ctaacttgtc taaattaacc
    11041 aataatccca agtgaaaagc tgcagaagca aaagtcctat caagtggctg tgtacacaca
    11101 ctacgaaact caactgttcc tcgagtcgtt aaatcttggt actggtaact acgatgagtt
    11161 tcaaaatcct tctcttgggg gtaaataata acctcatccc cattcagagc aaatgcttgg
    11221 atttcggacg tagccaaata gtccccagcc tgaataggat aaaaataata ggtctgccca
    11281 tcacgttccg cagtaaaaat cgcagaatga tttagatagt caaaaaaatc agcttcatca
    11341 ttaaggagtc tagcattgac cccaacattc tctggataga taccatgcat agattcttcc
    11401 cagaaaatat cccttgaaat tttcgtatcc caatccgcac ccgaaaattc agagtttgca
    11461 aataaataag ccttagccgc ttcaatttga gtaaaagcat taatcacccg taagtagttg
    11521 gtttttgaaa tatccagctg aacctggctc ccacagataa aagtaccata ttcagggaaa
    11581 tgatgtaaat ctgatttaat aatatttcta ctcaaattca aataatccat caacatctga
    11641 tagcgtggat aagccactgg acaattctca tttttatccc agttgggatg gataccacag
    11701 ccaacaatag catgatttga ttcagctaac tttctctgaa ttacattcat ataattattg
    11761 aaacgatttt cgacctcttg aatcgtttca gccttaccaa atgcaaactc aatcgttgta
    11821 taggaaactt caaataaaat agcatcctga cttatcggat ctactaactg gatcagattg
    11881 ccaaaatcat ctacctttgc tacggtgaga tccaaagtag aaactaaata atgaaataga
    11941 tccttcataa cttcaacatc tgtagcatcc ccttctaaac ttgcaacagg atactccaac
    12001 tcaattccga caaacaattc aggattttct ttaatatttt tcaaataccg atgttttaat
    12061 aaatcgatag aacaagacat actcccctac actttcttaa tctaaataaa atttgaataa
    12121 ctataattat accaaaaata atataaaaaa ggaacgaaga ataacaacgt tcctaacact
//
LOCUS       DAVMEI010000053        11766 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVMEI010000053 DAVMEI010000000
VERSION     DAVMEI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.053"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            248..2539
                     /gene="pcrA"
                     /locus_tag="VYQ24_001088"
     CDS             248..2539
                     /gene="pcrA"
                     /locus_tag="VYQ24_001088"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1968236.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2582..3262
                     /gene="radC"
                     /locus_tag="VYQ24_001089"
     CDS             2582..3262
                     /gene="radC"
                     /locus_tag="VYQ24_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1968237.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3259..3948)
                     /locus_tag="VYQ24_001090"
     CDS             complement(3259..3948)
                     /locus_tag="VYQ24_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1968238.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3966..4605)
                     /locus_tag="VYQ24_001091"
                     /pseudo
     CDS             complement(3966..4605)
                     /locus_tag="VYQ24_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
     gene            complement(4688..4903)
                     /locus_tag="VYQ24_001092"
     CDS             complement(4688..4903)
                     /locus_tag="VYQ24_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1968239.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4911..5258)
                     /locus_tag="VYQ24_001093"
     CDS             complement(4911..5258)
                     /locus_tag="VYQ24_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1968240.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5263..6378)
                     /locus_tag="VYQ24_001094"
     CDS             complement(5263..6378)
                     /locus_tag="VYQ24_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1968241.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6388..7347)
                     /locus_tag="VYQ24_001095"
     CDS             complement(6388..7347)
                     /locus_tag="VYQ24_001095"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1968242.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7460..8029)
                     /locus_tag="VYQ24_001096"
     CDS             complement(7460..8029)
                     /locus_tag="VYQ24_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1968243.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8157..8828
                     /locus_tag="VYQ24_001097"
     CDS             8157..8828
                     /locus_tag="VYQ24_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1968244.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8812..9630
                     /locus_tag="VYQ24_001098"
     CDS             8812..9630
                     /locus_tag="VYQ24_001098"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1968245.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9627..10523
                     /locus_tag="VYQ24_001099"
     CDS             9627..10523
                     /locus_tag="VYQ24_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1968246.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10567..11541
                     /gene="pta"
                     /locus_tag="VYQ24_001100"
     CDS             10567..11541
                     /gene="pta"
                     /locus_tag="VYQ24_001100"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1968247.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3425 a         2259 c         2533 g         3549 t
ORIGIN      
        1 ttcggattac gaatgaattt gattgctggt attatcaatc atgaactagg attctagttt
       61 tgcaggaagt ctattgaggt attgagctag tttatgaaaa aattgggtga aaagtcgagt
      121 gttttagaaa cccacagtgt agtattctag tttcaatcca ctatattttg ctactccccg
      181 taaagtttct attttccctg atttctgata taatagaaat attgacttca agagtaagga
      241 agagaagatg aacgcattat taaatggaat gaatgaccgt caggctgagg cggtgcaaac
      301 gacagaaggt cccttgctaa tcatggcagg ggctggttct ggaaagactc gtgttttaac
      361 ccaccgtatc gcttatttga ttgatgaaaa gctggtcaat ccttggaata tcttggccat
      421 tacctttacc aacaaggctg cgcgtgagat gaaagagcgt gcttatagcc tcaatccagc
      481 tactcaggac tgtctgattg cgaccttcca ctccatgtgt gtgcgtattt tgcgtcgcga
      541 tgcggaccat attggctaca atcgtaattt tacaattgtg gatcctggtg aacagcgaac
      601 gctcatgaaa cgtattctca aacagttgaa cttggatcct aaaaaatgga atgaacgaac
      661 tattttgggg accatttcca atgctaagaa tgatttgatt gatgatgttg cttatgctgc
      721 ccaagctggc gatatgtata cgcaaattgt ggcccagtgt tatacagcct atcaaaaaga
      781 acttcgtcag tctgaatccg ttgactttga tgatttgatt atgctgacct tgcgtctctt
      841 tgatcaaaat cctgatgttt taacctacta ccagcaaaaa ttccaataca tccacgttga
      901 tgagtaccaa gataccaacc acgctcagta ccaattggtc aaactcttgg cttcccgttt
      961 taaaaatatc tgtgtggttg gggatgcgga ccagtctatc tacggttggc gtggtgctga
     1021 tatgcagaat atcttggact ttgaaaagga ttaccccaaa gccaaggttg ttttgttgga
     1081 ggaaaattac cgctcaacca aaaccattct ccaagcggcc aacgaggtta ttaaaaacaa
     1141 taaaaatcgc cgtcctaaaa atctctggac tcaaaacgct gatggggagc aaatcgttta
     1201 ctatcgtgcc gatgatgagc tggatgaggc tgtatttgta gccagaacca tcgatgaact
     1261 tagtcgcagt caaaacttcc ttcataagga ttttgcagtt ctctatcgga ctaatgccca
     1321 gtcccgtaca attgaggaag ccctgctcaa gtctaacatt ccttatacca tggttggcgg
     1381 aaccaaattc tacagccgta aggaaattcg cgatattatt gcttatctca accttattgc
     1441 taatttgagt gacaatatta gttttgagcg tattatcaac gagcctaaac gtggaattgg
     1501 tccaggtaca gttgagaaaa tccgtgattt tgcaaatttg caaaatatgt ctatgctgga
     1561 tgcttctgct aatattatgt tgtctggtat caagggtaag gcagcccaat ctatctggga
     1621 ttttgccaat atgatgcttg atttgcggga gcagctagac cacttaagca ttacagagtt
     1681 ggttgagtcc gtcctagaaa aaacaggtta tgtcgatatt cttaacgccc aagcgactct
     1741 agaaagcaag gcacgggttg aaaatatcga agagtttctt tctgttacga agaactttga
     1801 tgacaccact gatgtgacag aagaggaaac tggtctggac aaactgagtc gtttcttaaa
     1861 tgacttggct ttgattgccg acacagattc aggtagtcag gagacatcag aagtgacctt
     1921 gatgaccctg catgctgcca aaggtctcga atttccagtt gtctttttga ttgggatgga
     1981 agaaaatgtc tttccactta gtcgtgcgac tgaagatcca gatgaattag aagaagagcg
     2041 ccgtctagcc tatgtaggta tcacgcgtgc agagaaaatt ctctatctga ccaatgccaa
     2101 ctcacgcttg ctttttggtc gtaccaatta taaccgtccg actcgtttta ttaacgaaat
     2161 cagttcagac ttgcttgagt atcaaggtct ggctcgtcct gcaaatacaa gctttaaggc
     2221 atcatatagc agtggtagta tttcctttgg tcaaggtatg agtttggctc aggctcttca
     2281 agaccgtaaa cgcggtgctg ccccaaaatc aatccagtca agcggtcttc catttggtca
     2341 atttacagct ggcgcaaaat cagcatctag cgaggcaaat tggtccattg gtgacattgc
     2401 tctccacaag aaatggggag agggaactgt tctggaagtt tcaggtagcg gtgctaggca
     2461 ggaattgaaa atcaatttcc cagaagtagg tttgaaaaaa cttttagcca gtgtggctcc
     2521 aattgagaaa aaaatctaat tttccatcct tctcacgaat aataaagtga ggaggatttt
     2581 tatgtacagt atttcattcc aagaagattc actattacca agagaaaggc tggccaagga
     2641 aggagttgaa gcgcttagta accaagagtt gctagctatt ttactcagga caggaacacg
     2701 tcaagctagc gtttttgaaa ttgcccaaaa agtcttgaac aatctttcaa gcctaacgga
     2761 tttgaaaaaa atgaccctgc aggaattgca gagtttgtct ggtattgggc gtgttaaggc
     2821 catagaatta caagctatga ttgaactggg gcatcgtatt cacaaacacg agactcttga
     2881 aatggaaagt attctcagca gtcaaaagtt ggccaagaag atgcagcagg aattagggga
     2941 taaaaaacaa gagcacctgg tggcactcta tctcaatact caaaatcaaa tcatccatca
     3001 gcagaccatt tttatcggtt ctgtaactcg tagtatcgct gaaccgcgag agattcttca
     3061 ctatgcaatc aagcatatgg cgacttctct tatcttggtc cacaatcatc cttcaggagc
     3121 ggtagcgcct agccaaaatg atgatcatgt cactaaactt gttaaagaag cctgcgaact
     3181 gatggggatt gttctcttgg accatttgat tgtctctcat tctaattact ttagttatcg
     3241 tgaaaagaca gatttaatct aaagttcatt aacgacatag tcaaagagtt tcttatcttt
     3301 gggacgattt tcaaaaagaa gttctggatg ccattggaca ccgagaaagg cgacatcatc
     3361 cgtactcatg acagcctcaa tgataccatc tttaggatca tgagccacaa cttttaaatt
     3421 tggtgctaag tccttgatgc tctggtggtg gaaggagttg atatgagaga tttctccata
     3481 gatttcttgg agaacggtat ctggttctgt taccaagcgt tgagttgtgt actcaacaga
     3541 agaatcctgc caatggtctt cgatatcttg gtacaaagtt ccacccatgg caacgttaaa
     3601 gagttgggta ccacggcaga cagagaaaat gggctttttc tgtttaatag cttccttgat
     3661 gagggccagt tcgaagatat ctctttgaag gtgataatca tcgctatcaa tggttttggg
     3721 ttcgccatag aattttggat cgacattttg cccacctgtc aagatgagct tgtcaatcaa
     3781 actgatatag tggcaggcca tttcttgatc accaatcggt aggatgatag gaatccctcc
     3841 agcatcttta acgccttcaa caaagccttt tgctgcgtag ctcatcatga tgtcatcatc
     3901 tggatgagtt ttttcgtttc ctgtaatccc aataactggt tttttcataa aatgattttc
     3961 gctttctaat cctcttttcg catgaagtag aggagggttt ggagttcact tgtcaaatcg
     4021 acatactgaa cgaccacgtc ttttggtaaa tgcagatgga ctggtgaaaa actgagaatt
     4081 cctttcacac cagcatcaac caagagatta gcaacctctt gtgacttgac gctgggaaca
     4141 gttaggatag cagtcttcac atcagcatcc ttgattttat ccttgatctg agaaatcccg
     4201 taaatgggaa tcccgtcagg agtttgggta ccgacttcag gatggtcgtc taggtcaaag
     4261 gccatgataa tcttcatctt gttacgttcg tggaagcggt agtggagaag ggcatggccc
     4321 atatttccaa taccaaccag catgacattg gtaatagagt tgtcattgag caaatcggca
     4381 aaaaatgtca ttagtttttt gacatcatag ccaaaaccac gacgaccaag ttcaccaaaa
     4441 taggaaaaat cacgacgtac ggtcgctgaa tcaataccga tagcctctgc aatttgctta
     4501 gagttggcac gttcaatctt ttctgcatga aatctcttaa aaattcgata gtagagagag
     4561 tctttttgct gtagcttttg gaatagcaaa ctgtttatct ttcacaaaat cacaaccttt
     4621 ctattcttct attttataga aacattgtga aaaaatcaac aaaaataaga aaaaactaag
     4681 aaaaatctta gttttgatgt aaaaaatctg catgagatag aaaacggtag aggtctccga
     4741 ccagaccctg ataaactttt tggcccctaa aagtcagaga agtcacataa agtgtatctg
     4801 gtaaggttac acatcctgac aaagtcaaca tgagagcctc atgatcctca tacttgagag
     4861 tacgctctac atgatagcag tccttatagg tcagttcaaa cattttggct ctatctttcc
     4921 gattttgtaa agacaccacg ttctaccaag ctatccatga ggaagtagaa tttttcctga
     4981 tgaatatggt ggtcttctga tttgaaaata tcaactagac gaaggccaaa cttgtcagtg
     5041 atattgattt tagcccctgt aagttccttg ttaatgatga ttttgagttg gaagccttca
     5101 ccgctgtttg gcactttttc caaaaggcga gtcagttcat agttaccaac cttagtttca
     5161 aaaaaggtgt tatctttgag ggtgaatttt ttaacagaag ggctaagagt gtaatcgtaa
     5221 cgacaatttt ttaactgaat gattttttca aatgccatat ggctaacctc cgataatttc
     5281 ttttaaggtt tttgcgaggg tttgtaggtc ttcaacggta ttttgtggcg acaaactgat
     5341 gcgaagggat tccttcaagc gttctgaatt tgcgccatac atggcttcaa gaacatggct
     5401 ggattggaca acgcctgcag tacaggctga gccagtagag attgaaattc cagctaaatc
     5461 tagccgaagg agtaagaggt catttttctg accaggaaat ccaatattga gaacataagg
     5521 gagatgatgt tttcctctat tcaagtaata ctgaatgccc tccagctctg ccagaaaggc
     5581 agtttctaga ttttgtacat gttgaaaatg ttcttcttgt ttttctaggt cttcttttag
     5641 ggctgcaacc atgcctacga tagcaggcag attttcagtt cctgcacgtt ttttctgttc
     5701 ctggtctccg ccatgtagat aggaatcaaa gtccatgcta gatgcgtaga gaaaaccgat
     5761 tcccttagga ccatggaatt tgtgggcaga agcagtgaga aaatcaatgc ccaattcttc
     5821 tgaatgaatt gggattttac caatagcctg aactgcatca acatgatagg cagcagggtg
     5881 ttgcttgagt atttggccaa tttcagcgat gggcagtagg tttcctgtct cattattggc
     5941 aaacatggta gaaaccaaaa tcgtatcgtc acgtaaagcc ttttgaattt gctgggctgt
     6001 gatttcttga ttttctggct ggataatggt tgcttcaaac ccaaagtgtt gaaccaagta
     6061 atcaattgtt tcaaggacag catggtgctc gatggcagtt gtgatgatat gttttccttg
     6121 ttcttggtga cgaagacagt agccaatgat ggtagtatta ttgccttcag tcccaccaga
     6181 agtgaaaaag atatgttgag gttttgtcct tagtaactgg gctagttcct gacgggcttc
     6241 tcgcaagagt ttgccagctt gacgaccatg accatgaata ctagaaggat ttccgtgggt
     6301 ttcttgcata accttggtca tagctgaaat agcaactgct gacataggag tcgttgcagc
     6361 attgtccaaa taaatcaaag aatcacctta tttcttttta ttgtaggcaa agagtgggct
     6421 gactggtttt ctttcgtgaa tacggacgat agcatcacca attaactcac tagcagtgat
     6481 gtagcataca tttttaggag ttttttcttt tgttgctact gaatcagtca caagaatttc
     6541 tttaatatta gtattgtcaa gaagctcagc agctccctcg acgaagagac cgtggctaga
     6601 aacagcataa atttctgtag ctccttcacg ttcaacgatt ttagaagctt cagagaaggt
     6661 acgtcctgta tttaaaatat catcaatcaa gatagctttc ttaccttcaa catcaccaat
     6721 aatataacct tcgttacgag ttgcatcgtc ttgagggtag tcgataatgg cgataggagc
     6781 atcaagatat tcagccaggc tacgcgcacg tttgacacct gaatttttag ggctaacgac
     6841 aacaacatct gaaccaagca atcctttatc gcagtaatgt tttgcgaata ggggaacagt
     6901 gaaaagatta tccactggaa tatcaaagaa accttgaacc tgaacggcat gcaaatcaag
     6961 agtcaggata cgatcaactc cagccttaac cagcatattg gcaactagtt ttgctgtaag
     7021 tggctcacga ggacaagcaa tgcggtcttg acgtgcatag ccaaaatatg gaaggacaac
     7081 gttgatactg tgggcacttg cacgcacaca agcatcgacc atgattaaca attccattag
     7141 gtggttgttg acagggaaac ttgttgattg gatgatgtaa acatcataac cacggacact
     7201 ttcttcgata tttacttgga tttctccgtc tgaaaattga cgtgatgata gttttccaag
     7261 tgggacacca acagcttggg caattttttg tgcaatctct tggttagagt tgagtgcgaa
     7321 aagtttcatg ttttttctat ctgacattat agaccgtcct ctgtaaactt tataaatcct
     7381 agttatattt accttacata tatgaactgg gatttgtgta tttttatctt ttctatttta
     7441 ccaaaaaatg gagattattt cagctatttt tcatactttt gacaaatcga accaattttg
     7501 aaggagcttt ttggtaggaa atctgatttt tctctaaaaa ttgtcggaaa tcctgtttgc
     7561 cttgctcatg attttccact tcaagctcca attcgtaatc tgttatatca aagtatcggc
     7621 tctgatccag tgccatgaga ccaatagctg ttttcatttc atagcgaagc gttgttagac
     7681 aaccaagaac ctgccagttc ttactttgga taccatgttt cgccaattca tccagtacta
     7741 gcccttgagg aagttcttcc ttactcagat agttctcagc atcttttagt tgcaattttt
     7801 ggttgtattc catgtttcca acactctgcg ggactttgag tgtcaactca gcccagtctt
     7861 caaaggttcg aatgcgcata gcgactttct tttctcgcag ttcaaaatca ggcgtgtcga
     7921 tgtagtaatt tgtttgaaga acaggagtga cacctgtgaa ctggtctttt agacgattgt
     7981 attcatcttt tttcaatagt gttttcaatt caatttctaa atgtttcatt tttcttacct
     8041 ttttttatcg ttgaaagcgg atttatggta taataagcat tgtatttatt gtatatgaat
     8101 ctggagaaaa aatcaaagat atttttgacg gataatatga gaacaaggga gaatatatga
     8161 ccttagaatg ggaagaattt ctagatcctt acattcaagc tgttggtgag ttaaagatta
     8221 aacttcgtgg tattcgtaag caatatcgta agcaaaataa gcattctcca attgagtttg
     8281 tgaccggtcg agttaagcca attgagagca tcaaagaaaa aatggctcgt cgtggcatta
     8341 cttatgcgac cttggaacac gatttgcagg atattgctgg cttacgtgtg atggttcagt
     8401 ttgtagatga cgtcaaggaa gtagtggata ttttgcacaa gcgtcaggat atgcgaatca
     8461 tacaggagcg agattacatt actcatagaa aagcatcagg ctatcgttcc tatcatgtgg
     8521 tagtagaata tacggttgat accatcaatg gagttaagac tattttggca gaaattcaaa
     8581 ttcgtacttt ggccatgaat ttctgggcaa cgatagaaca ttctctcaac tacaagtacc
     8641 aaggggattt cccagatgag attaagaagc gactggaaat tacagctaga atcgcccatc
     8701 agttggatga agaaatgggt gaaattcgtg atgatatcca agaagctcag gcactttttg
     8761 atcctttgag tagaaaatta aatgacggtg taggaaacag tgacgataca gatgaagaat
     8821 acaggtaaac gaattgatct gatagccaat agaaaaccgc agagtcaaag ggttttgtat
     8881 gaattgcgag atcgtttgaa gagaaatcag tttatactca atgataccaa tccggatatt
     8941 gtcatttcca ttggcgggga tggtatgctc ttgtcggcct ttcataagta cgaaaatcag
     9001 cttgagaagg tccgctttat cggtcttcac actggacatt tgggcttcta tacagattat
     9061 cgtgattttg agttggacaa gctagtgact aatttgcagc tagatactgg ggcaagggtt
     9121 tcttaccctg ttctgaatgt gaaggtcttt cttgaaaatg gtgaagttaa gattttcaga
     9181 gcactcaacg aagccagcat ccgcaggtct gatcgaacca tggtggcaga tattgtaata
     9241 aatggtgttc cctttgaacg ttttcgtgga gacgggctaa cagtttcgac accgactggt
     9301 agtactgcct ataacaagtc tcttggcggt gctgttttac accctaccat tgaagctttg
     9361 caattaacgg aaattgccag ccttaataat cgtgtctatc gaacactggg ctcttccatt
     9421 attgtgccta agaaggataa gattgaactt attccaacaa gaaacgatta tcatactatt
     9481 tcggttgaca atagcgttta ttctttccgt aatattgagc gtattgagta tcaaatcgcc
     9541 catcataaga ttcactttgt cgcgactcct agccatacca gtttctggaa ccgtgttaag
     9601 gatgccttta tcggtgaggt ggatgaatga ggtttgaatt tatcgcagat gaacatgtca
     9661 aggttaagac cttcttaaaa aagcacgagg tttctaaggg attgctggcc aagattaagt
     9721 ttcgaggtgg agctattctg gtcaataatc aaccgcaaaa tgcaacgtat ctattggacg
     9781 ttggagacta cgttaccatt gacattcccg ctgagaaagg ctttgaaacc ttggaggcta
     9841 ttgagcttcc attagatatt ctctatgagg atgaccactt tctagtcttg aataaaccct
     9901 atggagtggc ttctattcct agtgttaatc actctaatac cattgccaat tttatcaagg
     9961 gttactatgt caagcaaaat tatgaaaatc agcaggttca cattgttacc agactagata
    10021 gggacacttc tggcttgatg ctctttgcca agcacggtta tgcccatgca cgattagaca
    10081 agcagttgca gaagaaatct atcgagaaac gctactttgc tttggttaag ggagatggac
    10141 atttggaacc agaaggggaa attattgctc cgattgcgcg tgatgaagat tccattatta
    10201 ccagacgagt ggctaaaggc ggaaagtatg cccatacttc atacaagatt gtagcttctt
    10261 atggaaatat tcacttggtc tatattcacc tgcacactgg tcgaacccat caaatccgag
    10321 tccatttttc tcatatcggt tttcctttgc tgggagatga tttgtatggt ggtagtctgg
    10381 aagatggtat tcaacgtcag gctctgcatt gccattacct atccttttat catccatttt
    10441 tagagcaaga cttgcagtta gaaagtccct tgccggatga tttcagtaac cttattaccc
    10501 agttatcaac taatactcta taaaaactgt ctcagagtat aattattatc ttaaaggaga
    10561 aaactcatgg aagtttttga aagtctcaaa gccaaccttg ttggtaaaaa tgctcgtatc
    10621 gttctccctg aaggggaaga gcctcgtatt cttcaagcaa caaaacgctt agtaaaagaa
    10681 acagaagtga ttcctgtttt gcttggaaat cctgaaaaaa ttaaaattta tcttgaaatt
    10741 gaaggaatca tggatggtta tgaggtcatc gaccctcaac attatcctca atttgaagaa
    10801 atggtttctg ccttggtgga gcgtcgcaag ggcaaaatga ctgaagaaga tgtacgcaag
    10861 gttttggttg aagatgtcaa ctactttggt gtgatgttgg tttacttggg cttggttgat
    10921 ggaatggtgt caggagcgat tcactcaaca gcttcaacag ttcgcccagc tctacaaatc
    10981 atcaaaactc gtccaaatgt aactcgtact tcaggagcct tcctcatggt tcgtggtacg
    11041 gaacgttacc tatttggaga ctgtgccatt aatatcaatc cagatgcaga agccttggct
    11101 gaaattgcca tcaactcagc aatcacagct aagatgtttg gcatcgaacc taaaattgcc
    11161 atgttgagct attctactaa aggttcaggg tttggtgaaa gcgttgataa ggtcgttgaa
    11221 gcaactaaaa ttgctcacga cttgcgtcct gaccttgaaa tcgatggtga gttgcaattt
    11281 gatgcggcct ttgttcccga aactgcagct ctgaaagctc cgggaagtac agtagctggt
    11341 caagcaaatg tcttcatctt cccaggtatc gaggcaggaa atatcggtta caagatggct
    11401 gaacgcctgg gtggctttgc ggctgtagga cctgttttgc aaggtttaaa caagccagtt
    11461 aatgatcttt ctcgtggatg taatgcagat gatgtttaca agttgaccct catcacagca
    11521 gctcaagcag ttcatcaata gtgaaaacta taaagtgata tactatgcta tactgtagtt
    11581 atgaaactat gtacgaaaag cactgccatt aattcctgag aactaaatta ctgattggtg
    11641 tcaaaaagga aaacttccaa gcgatgatat cctgtctata cacgacctat agaaatctgt
    11701 aatatacata tccgtaaaac gataaattcc ctttttgatt ttaaatgagt atgaaaagag
    11761 aatttt
//
LOCUS       DAVMEI010000054        11588 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVMEI010000054 DAVMEI010000000
VERSION     DAVMEI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.054"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            168..821
                     /locus_tag="VYQ24_001101"
     CDS             168..821
                     /locus_tag="VYQ24_001101"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1968248.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            831..1295
                     /locus_tag="VYQ24_001102"
     CDS             831..1295
                     /locus_tag="VYQ24_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1968249.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1308..2576
                     /locus_tag="VYQ24_001103"
     CDS             1308..2576
                     /locus_tag="VYQ24_001103"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1968250.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     NATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2590..2850
                     /locus_tag="VYQ24_001104"
     CDS             2590..2850
                     /locus_tag="VYQ24_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968251.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2861..4222
                     /gene="pdrM"
                     /locus_tag="VYQ24_001105"
     CDS             2861..4222
                     /gene="pdrM"
                     /locus_tag="VYQ24_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1968252.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4376..5344
                     /locus_tag="VYQ24_001106"
     CDS             4376..5344
                     /locus_tag="VYQ24_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1968253.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGSVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5360..6352
                     /locus_tag="VYQ24_001107"
     CDS             5360..6352
                     /locus_tag="VYQ24_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1968254.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEEISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCSGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6570..7613
                     /locus_tag="VYQ24_001108"
     CDS             6570..7613
                     /locus_tag="VYQ24_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1968255.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7677..9362
                     /gene="lpdA"
                     /locus_tag="VYQ24_001109"
     CDS             7677..9362
                     /gene="lpdA"
                     /locus_tag="VYQ24_001109"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1968256.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9426..10415
                     /locus_tag="VYQ24_001110"
     CDS             9426..10415
                     /locus_tag="VYQ24_001110"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1968257.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10488..11558)
                     /gene="xerS"
                     /locus_tag="VYQ24_001111"
     CDS             complement(10488..11558)
                     /gene="xerS"
                     /locus_tag="VYQ24_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1968258.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAKNIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3417 a         2180 c         2618 g         3373 t
ORIGIN      
        1 aatgatttaa aaagtaatta taaaaaatag ccagtcttta taaactgtat tcacgtattc
       61 aaatcgtata tatagtcgat ttattaaatc tttagataaa ctgtaaaaag tggtttccat
      121 agccactttt tgctataata gaggcagtaa aaatgaagga gtcagctatg gaacactcgt
      181 cttggcatgc tttgattaag gcgcaattac ctgagggtta tttcgggaaa atcaatcagt
      241 ttatggagca ggtctattct caggggatta tttatccacc caaggaaaag gtttttcagg
      301 ctctcttgac aacactgctt gaagaagtta aggtggtaat tctagggcaa gacccctatc
      361 acggaccagg tcaagcgcag ggcttgagtt tttctgtacc tgactctatc ccagctccac
      421 catccttgca aaatatcttg aaagaattgt cagatgatat cggagttaag aaatctcatg
      481 atttgacagc ttgggctgag caaggagtct tgcttcttaa tgcttgtttg actgttcctg
      541 ctggacaggc caatggtcat gctggtcaaa tctgggagcc ttttactgat gctgtgattc
      601 aggtggtcaa tcatctagat agaccagtcg tttttgtact ctggggagct tatgcacgta
      661 agaagaaggc cttagttacc aatcctcatc acttgattat tgaatcagcc catccaagtc
      721 ctttgtcggt ttatagagga ttttggggtt ccaagccctt ttccaaggcc aatgcattct
      781 taaaagagac aggacaagag ccaatcgatt ggcttagata aggagaaaat atgcctcagt
      841 tagcgacgat ttgctacatt gataatggga aagaactgct catgctccat cgtaataaga
      901 aacccaatga tgtccatgaa gggaaatgga ttggtgtggg tggtaagcta gagagaggag
      961 agacgcccca ggaatgcgcg gcgcgtgaaa tccttgaaga aacagggctc aaagccaagc
     1021 cagttctaaa aggtgtcatc acttttcctg aatttacacc agatttagac tggtacacct
     1081 atgtttttaa ggtgacggag tttgagggcg acttgattga ctgcaatgag gggacgctag
     1141 aatgggttcc ctatgacgag gttttgagca agccgacttg ggaaggtgac cacacctttg
     1201 ttgagtggct tttagaggat aaacccttct tttcagccaa gtttgtttat gatggggata
     1261 aattgttgga tacccaagtt gatttctatg aataaaggag aaagcagatg ctactaatca
     1321 aaaatggtcg tgtaatggat cctaagtctg gtttggatca agtttgtgat gtcttagttc
     1381 aagatgggaa aattatcaaa attgcgcctg agatcacgga agaaggagca gaaacgatta
     1441 atgctactgg tcttgtggtt gctcctggtt tggtcgatat tcatgtgcat ttccgtgaac
     1501 ctggtcaaac acataaagaa gacattcata ctggtgccct agcagccgct gcaggtggtt
     1561 ttactactgt cgtcatgatg gctaatacca gtccaaccat ttcagacgtg gagactttgc
     1621 aagcagttct ccagtcagct gccaaagaga agattaatgt caagacagtt gcgaccatta
     1681 ctaaaaactt taatggtaaa aacttgactg actttaaggc gctcttagaa gctggtgcgg
     1741 ttggtttctc tgatgacggt attccgcttg agagcagtaa gattgtcaag gaagccatgg
     1801 aggaagccaa aaaactcaat acctttatca gccttcatga ggaagatcca ggtttgaacg
     1861 gtgttcttgg ctttaatgaa aatattgcta gagaacattt tcatatctgc ggtgctactg
     1921 gggtggctga gtacgctatg atggcgcgtg atgtcatgat tgcctatgca actaaagccc
     1981 atgttcacat ccagcatttg tctaaggaag aaagtgttaa agtagtggag tttgctcagg
     2041 ggttaggtgc agaagtcaca gcagaagtag cgccacagca tttctctaag acagaagcac
     2101 ttcttttaac acaaggtagc aatgctaaga tgaatccacc gcttcgtttg gaatcagacc
     2161 gtcgtgccgt tatcgaaggt ctcaaatcag gtgtcatcac agttattgcg actgaccacg
     2221 cgcctcatca tgtagatgaa aaaaatgttg aggatattac caaagcgcca tctggtatga
     2281 ctggcttaga aacatccctg tctctcagct tgacctattt agtagaagct ggtgagttga
     2341 gcttgatgga attacttgaa aaaatgacat acaacccagc caagctttac aactttgaag
     2401 caggttactt ggctgagaat ggtccagcag atatcactat ttttgatgcc aaggctgacc
     2461 gccttgtgga ctcccatttt gcttccaaag cagctaattc accattcatc ggtgaaacct
     2521 taaaagggca ggttaaatat accatctgta agggacaaat cgtctatcaa gcttgatagg
     2581 agttagaata tgaattattt tcgaaaacgt agggagagac aagcgaaatc taatagtgga
     2641 atttactgtc ccgcagctaa tcgttccaaa attcaatatg aaactaaaga aaaagctgac
     2701 catgcggttc aatatgataa cggaggttta gttagaagct attattgtcg tacatgtgcg
     2761 tgttggcata ctacttcaaa atctaataaa cctccgctaa gtttgaaaaa ctatggtctg
     2821 aaactctttg gtttagataa atcagaatag aaagagaagt atgtataaga caaagtgttt
     2881 acgagagaag ttagtattat ttttaaaaat tttcttccca atactgatct accaatttgc
     2941 caattattct gcctcttttg ttgatactgc aatgacaggt caatacaaca ctatggactt
     3001 ggctggtgta tctatggcaa ccagtatctg gaatcctttc tttacatttc taacagggat
     3061 tgtgtcagcc ttggtgccta tcattggtca ccatcttggt cgaggaaaaa aggaagaagt
     3121 tgcgtctgat ttttaccaat ttatttattt ggccttgggc ctatctgtgg tcttgctggg
     3181 gatggtactt ttcttggcac caacaatctt gaatcatatt gggttagaag cagcagtagc
     3241 ggcagtagcg gttcgctatc tttggttttt atctatcggg attatcccct tgttgctctt
     3301 tagcgtcatt cgttccttgc tggattcgct gggcttgacc aaactgtcca tgtacctcat
     3361 gcttttgtta ctccctctca atagcggatt taactatctc ttgatttacg gtgcctttgg
     3421 tgttccagaa ctgggagggg ctggtgctgg tttaggaaca tccttggcct actgggtctt
     3481 gcttgggatt tctgttctgg ttttatttaa acaggagaag ctcaaagcct tacaccttga
     3541 gaaacgaatt ccacttaata tggataaaat taaggaagga gttcgtttag gtctgcctat
     3601 tgggggaact gtcttcgcgg aagtggctgt cttttcagtg gttggcttga ttatggctaa
     3661 gttttcgtcc ttgattatag ctagtcacca gtcagctatg aacttttcaa gtcttatgta
     3721 cgcctttcct atgagtatct catcggctat ggctattgtc gtttcctatg aagtgggagc
     3781 caagcgattt gatgatgcga aaacctatat tggtctagga agatggactg ccctcatttt
     3841 tgcggccttc accttaacct tcctttacat ttttagggga aatgtggcca gtctttatgg
     3901 taacgaccca aaatttatcg atttgacagc gcgtttttta acttatagtc ttttcttcca
     3961 gttagcagat acctttgcgg cgccgcttca gggaattttg cgggggtata aggatacagt
     4021 tattcctttt taccttggtt tgcttggtta ttggggcgta gcaatccctg tggctacgct
     4081 atttgattcc ctaacagatt ttggagctta ttcttactgg atcggcttga ttattagttt
     4141 gattgtgagc ggggcgctct accgttggcg tttaactgtg attatgaaga gatttgaatc
     4201 tttagcaaaa tccaaacgct aaaaagtttg tgaaaaaata ttcttgacaa gaaaatccct
     4261 tgttttcacg gggttttctt ttttattttg tgaaatttct tttagaaaaa ctttgacagt
     4321 atttgccaaa agcggtaaaa tagtaaggta agaagaaagt tagaaaggca agaagatgtc
     4381 aactttagat aaaaatcttt tgctagaaat gttccgtaag atggaagaaa tccgtcgcat
     4441 ggacttaaaa attgcacaat tagtaaagaa agggaaagtg ccaggaatga cgcacttttc
     4501 tgttggtgaa gaggcagcta acgtgggggc tatgttagct ctcaatccag atgatctgat
     4561 tacctcaaac caccgtggac acgggcaagc tattgctaaa gggattgacc tcaacggaat
     4621 gatggctgaa atccttggta aatacactgg aacctgtaaa gggaaaggtg gatctatgca
     4681 tatcgctgac cttgatgctg ggaaccttgg tgccaatggt atcgtaggtg gtggtatggg
     4741 gatcgctgtc ggtgcagccc tcagtcagca aatgcaaaat accggtaaaa tcgttgtttg
     4801 cttctttgga gatggtgcga ccaatgaagg tgttttccac gaagcagtga acatggcttc
     4861 tatctggaac ctgccagtca ttttctattg cattaacaac ggttacggta tctctgcgga
     4921 tatcaagaaa atgaccaata tagaacatat ccatcaacgt agcgccgctt atggaattcc
     4981 tggaatgttc atcgaagacg gtaacaatgt catcgatgtc tatgaaggat ttcagaaagc
     5041 tgtagaccat gttcgcagtg gcaatggttc agtcttgatt gaaagtgtaa cttatcgctg
     5101 gctcggtcac tcatcatctg accctggtaa atatcgtacg cgtgaagaag tggaattgtg
     5161 gaaacaaaaa gatccaatcg aaaacctccg caattacctt attgaaaata acattgcaag
     5221 tgccgaagaa ttggaagaaa tccaagcgca agtaaaggaa gcagtagaag cttctgttaa
     5281 atttgcagag gaaagcccat tcccaccgct tgaatcagcc tttgaagata tttacgcaga
     5341 ctaaggagaa agagataaaa tggaaacaaa aacaatgtcc ttccgtgaca ccattatcct
     5401 tgctatgtct gaggaaatgc gtcgcgatga aaatgtgttc ttgatgggag aagatgtcgg
     5461 tgtcttcgga ggagacttcg gtacttctgt tggaatgctt gaagaatttg gtccagaacg
     5521 tgtccgtgac tgtccgattt ctgaagctgc catctctgga gcagcagcag gagcagccat
     5581 gacaggactt cgtccaatcg tcgatatgac cttcatggac ttctctgtta ttgccatgga
     5641 caatatcgtc aaccaagctg ctaaaacacg ttacatgttt ggtggtaaag gtcaggttcc
     5701 aatgactgtt cgttgtgcag ctggtaacgg agttggctct gccgcccagc actcacaatc
     5761 tctagagtct tggtttactc acattccagg acttaaggtt gtggcacctg gaacacctgc
     5821 ggacatgaaa ggcctgctta agtcttctat ccgtgataac aacccagtta ttatacttga
     5881 gtacaagtca gaatttaacc aaaaagggga agtgccagtt gatccagact acacaattcc
     5941 acttggggtt ggtgaaatca aacgccaagg aacggatgta acagttgtta cttatggaaa
     6001 aatgcttcgc cgtgtggttc aagctgctga agaattagca gaagaagaaa tttcagttga
     6061 aattgttgac ccacgtaccc ttgttccgct tgataaggat atcatcataa actcagtgaa
     6121 gaagactggt aaggttattc tggtcaacga tgcccacaaa acaagtggct atatcggtga
     6181 aatttcagct attatttcag aatcagaagc atttgactat ctagatgcac caatacgccg
     6241 ttgttcagga gaagatgtgc cgatgcctta tgcacaaaac ctagaaaatg caatgattcc
     6301 aacagttgaa agtatcaaag atgcaatccg aaaaacttat aacaaagact agagttacat
     6361 aatataagtt atgtaactaa aataaagctg acgagaaact ttgtatcttt taggtgcaga
     6421 gctcttcgtc attaatatct tatattcgag cacgggctaa aagctcggaa aaaagataaa
     6481 tctccttggc ttcatcgaag ccatcgtcga tttcctattt ttcagtcgct tttgacgccc
     6541 tttgtatcat aaatagaatt ggagaggtca tggctgatga caagctaaga gcgactcctg
     6601 cagctagaaa gttagcggat gatttaggga taaacctcta cgacgtttct ggctcaggtg
     6661 caaacggtcg tgtccacaaa gaagacgtgg aaacttataa agacacaaac gtggttcgca
     6721 tttcgccact tgcaaaacga attgccctcg aacataacat tgcttggcag gaaatccaag
     6781 gaaccggtca tcgtggtaaa atcatgaaga aggatgtttt ggccctgctt cctgaaaata
     6841 tcgaaaacga tagcatcaaa tctcctgctc agattgaaaa agtggaagaa gtccctgata
     6901 acgtaacacc atatggtgaa atcgagcgta ttccaatgac accaatgcgt aaggttattg
     6961 cccaacgcat ggttgaatct tacttgactg cgccaacctt caccctcaac tatgaagttg
     7021 atatgactga aatgttggct cttcgtaaga aggttcttga gccaatcatg gaagcaactg
     7081 ggaagaagac tactgtaaca gaccttcttt cgcttgcagt tgttaagact cttatgaaac
     7141 acccatacat caacgcttca ttgacagaag atggcaagac tattatcact cacaactatg
     7201 tcaatcttgc catggcagtt gggatggata atggattgat gacacctgtt gtttacaatg
     7261 ctgagaagat gagtctttca gaactggtgg tagcctttaa ggatgttatt ggccgtacct
     7321 tggatggtaa attggctcca agtgagttac aaaattcaac attcacaatc agtaatttgg
     7381 gaatgtttgg tgttcagtcc tttggtccga ttattaacca acccaactca gctatccttg
     7441 gtgtcagttc gacaatcgag aagccagttg tcgtcaatgg tgaaattgtg attcgcccaa
     7501 tcatgagttt aggattaacc attgaccacc gtgtcgtaga tggtatggct ggtgctaagt
     7561 ttatgaagga cttgaaagag ttgattgaaa cgccaatctc aatgttaatt taagagcaag
     7621 agtattcatt tttaagatat agataaagga aggaagacat ggccttagaa ataattatgc
     7681 caaaagccgg cgtggatatg acagaaggac aaatcgtcca atggaataaa aaagtcggag
     7741 aatttgtaaa agaaggagaa atccttttgg aaatcatgac tgataaagtc agcatggaat
     7801 tggaagccga agaagatggg tacttgattg ccattctcaa aggagatggt gaaactgtcc
     7861 ctgtaacgga agttatcggt taccttggtg aagaaaggga aaacatccca acagctggag
     7921 cagcatcgcc agaagctagc cctgtacctg tagcaagtac ctcaaacgat gatggtaaga
     7981 gcgatgatgc ttttgatatc gttgtgattg gtggaggtcc tgctggttat gttgcagcca
     8041 ttaaagctgc ccaactcggc ggtaaggttg cccttgttga gaaatctgaa cttggtggaa
     8101 cctgcttaaa ccgtggatgt attccaacca agacctacct tcatagcgct gaaattattg
     8161 aaaatatcgg tcatgctgca aatcgtggta tcgtgattga aaatcctaac ttcactgttg
     8221 atatggaaaa acttttagaa actaaatcta aagttgttaa tactcttgtt ggtggtgttg
     8281 caggacttct tcgtagctac ggagttgctg ttcataaagg aattggtaca atcactaaag
     8341 acaagaacgt cttggtaaat ggttctgaat tgcttgaaac caagaaaatc attcttgctg
     8401 gtggttcaaa agtcagcaag atcaacgtcc ctggtatgga atctccactt gtcatgacta
     8461 gtgatgacat tcttgaaatg aacgaagtgc cagaaagcct tgttatcatc ggtggtggag
     8521 ttgtcggtat cgaactcggt caggccttca tgacatttgg ttcaaaagtg actgttatcg
     8581 aaatgatgga ccgtatagtt ccagctatgg atgtggaagt ttctaagaac cttcgcttga
     8641 tccttgagcg taaaggaatg accatcttga ctggtactaa actgcaagaa atcattgagg
     8701 aaaatggtca acttcgtatc aaggttgaag gaaaagacga tatcatcgca agcaaagctc
     8761 ttctttcaat tggtcgtatg ccagaccttg aaggtattgg agaggttgag tttgaattgg
     8821 atcgtggttg tatcaaggtc aacgaataca tggaaacttc agttccaggc atttatgcac
     8881 caggtgacat caacggtact aagatgttgg ctcacgcagc tttccgcatg ggtgaagttt
     8941 ccgctgaaaa tgcccttaaa ggaaatcatg cagttgccaa attgaatttg actcctgcag
     9001 ccatctacac tcttcctgaa gtagcagcag taggtttgac agaagaacaa gcccgtgaga
     9061 aatacgatgt tgccatcggt aagtttaact ttgctgctaa cggtcgtgct attgcatctg
     9121 acgcagctca aggtttcgta aaagttatcg ctgataagaa atacggagaa atccttggtg
     9181 tacacattat tggtcctgca gccgcagaat taataaacga agcatcaagc atcatcgaaa
     9241 tggaaatcac tgttgaggaa atgctgaaga ccatccacgg gcacccaact tactctgaag
     9301 tgatgtacga agcgtttgca gatgttctag gaatggccat ccattcacct aagaaaaaat
     9361 aaaacgaaga tagattagac tggaaggtta tttccagttt ctatcatata aagtaaaggg
     9421 atatcatgaa atacattatc aatcattcaa atgacactgc ttttaatatt gccttggaag
     9481 aatacgcctt taaacacctt ttggatgagg atcaaatctt cctactttgg attaacaagc
     9541 catctatcat tgttggtcgt caccagaaca ctattgaaga aatcaaccgt gattatgttc
     9601 gcgaaaatgg tattgaggta gttcgccgta ttagtggtgg tggagctgtt taccacgatt
     9661 taaataacct caactacacg atcatctcca aagaagatga aaataaggca tttgacttca
     9721 agagcttctc aactccggtt atcaatacct tggctcaact cggggttaaa gctgagttca
     9781 caggtcgtaa tgaccttgag attgatggta agaagttctg tggcaatgcc caagcctata
     9841 tcaacggccg tatcatgcac cacggttgct tgctctttga cgttgatttg tcagtcctcg
     9901 caaatgccct taaggtttca aaagataaat ttgaatcaaa aggtgtgaaa tccgtccgtg
     9961 cccgtgtaac caatattatc aatgaattac caaaaaaaat cacagtcgaa aaattccgtg
    10021 atttactctt ggaatacatg aaaaaagagt acccagagat gactgaatac gtcttttcag
    10081 aagaagaatt ggccgaaatc aatcgcatca aggatactaa gtttggaact tgggactgga
    10141 actatggtaa atcacctgaa tttaacgtcc gtcgtggaac caaattcact agtggtaagg
    10201 ttgaagtctt tgctaacgtt actgaatcaa aaatccaaga catcaagatt tatggtgact
    10261 tctttggtat tgaagacgtt gctgcagtag aagatgtcct tcgtggggtg aaatacgaac
    10321 gcgaagatgt tcttaaggcg ctaaaaacca ttgatatcac acgctacttc gctggtatta
    10381 gccgtgaaga aatcgctgaa gcggtagttg gataaatcaa aaagaagttg gccaactaac
    10441 caacttcttt ttttgatatt tatttacata atttaaaata cgtaaaatca taaactatcc
    10501 agagcattct tttgttcatc actaacaata tgggtataga ggtcagtgac ttgtgtgcta
    10561 gcatgtccta gttggtgact gactaaaact tgtgatttag tcgcatcata gagcctagtt
    10621 gctagtgtat ggcgcagttt atggggtgtt acacgcactt taaaatcctc tgagtattta
    10681 gcaaccattt tctcaacgct agaagcatcg atacgattag gaacacctct gtagagagtt
    10741 aaaaaaaggg ctgtatctgt tttttccgtt ttatagcgtt gattccgaat ggccagataa
    10801 ttctctaaat aaggtttagc aaaagcagcg acattgactg agtcacgttt gccacctttt
    10861 cgagtaacat caataaccat catttttaga ttgagatctc ttagatctag attaacagct
    10921 tcagataagc gaacaccaga tgccaagaga agggcaataa tggctaaatc tcgttcttta
    10981 tttttgttga atgatgagag agctcgattt gaaagctgtt gtgggtgctc ttgatcgata
    11041 taagttagaa aaccttctgt ttcatcacct agaaagagtt tttgcttgat atttttagct
    11101 ctggcagcaa gggtttcttt ctttttcttg gttgaaactt ttttcattac attacgatag
    11161 aaataaggtt ccccctgatc gttttcaacc tcttcggtta gatacttgta aagactagaa
    11221 agtgctgata aggttcgatt gatagttgtc tgtgaaacac cttgttttgt tgtattagca
    11281 ttcagcaagg gacgttcacg tagataaagg ataaaggatt ccatgtcttt cttagacata
    11341 ttttccaaaa ctgataaagg aatatcggat attttatcag cgtttgaaat accagactcc
    11401 aaaacccagc tgaaaaatcg gtcatattcc ttaaggtatt cgtacagggt tgtaaaactg
    11461 tagggcacag ccagcttaga ttggtagtat tccagaacat accagggcat gagttgtttt
    11521 agtttgtcga ttcgttccag taaaatctca cgtttcatgt attttctcca taagtaagta
    11581 tactagta
//
LOCUS       DAVMEI010000055        11086 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVMEI010000055 DAVMEI010000000
VERSION     DAVMEI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.055"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            65..880
                     /locus_tag="VYQ24_001112"
     CDS             65..880
                     /locus_tag="VYQ24_001112"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1968259.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            899..1540
                     /locus_tag="VYQ24_001113"
     CDS             899..1540
                     /locus_tag="VYQ24_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1968260.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            1571..2572
                     /locus_tag="VYQ24_001114"
     CDS             1571..2572
                     /locus_tag="VYQ24_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1968261.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            2582..3211
                     /locus_tag="VYQ24_001115"
     CDS             2582..3211
                     /locus_tag="VYQ24_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1968262.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(3590..3975)
                     /gene="tnpA"
                     /locus_tag="VYQ24_001116"
                     /pseudo
     CDS             complement(3590..3975)
                     /gene="tnpA"
                     /locus_tag="VYQ24_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(4331..5212)
                     /locus_tag="VYQ24_001117"
     CDS             complement(4331..5212)
                     /locus_tag="VYQ24_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1968263.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5353..6681)
                     /gene="vncS"
                     /locus_tag="VYQ24_001118"
     CDS             complement(5353..6681)
                     /gene="vncS"
                     /locus_tag="VYQ24_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1968264.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(6678..7334)
                     /gene="vncR"
                     /locus_tag="VYQ24_001119"
     CDS             complement(6678..7334)
                     /gene="vncR"
                     /locus_tag="VYQ24_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1968265.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7383..7466)
                     /gene="pep27"
                     /locus_tag="VYQ24_001120"
     CDS             complement(7383..7466)
                     /gene="pep27"
                     /locus_tag="VYQ24_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1968266.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(7646..9025)
                     /gene="vex3"
                     /locus_tag="VYQ24_001121"
     CDS             complement(7646..9025)
                     /gene="vex3"
                     /locus_tag="VYQ24_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1968267.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9077..9724)
                     /gene="vex2"
                     /locus_tag="VYQ24_001122"
     CDS             complement(9077..9724)
                     /gene="vex2"
                     /locus_tag="VYQ24_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1968268.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(9737..11014)
                     /locus_tag="VYQ24_001123"
     CDS             complement(9737..11014)
                     /locus_tag="VYQ24_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968269.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3157 a         2417 c         2193 g         3319 t
ORIGIN      
        1 accggtttac tagaatataa tttggttata gagaagtctt aacaaatttg aaaggaagta
       61 tcaaatgaca aatacatcat tctcaattga gcagttttct ttaaaaggaa aaattgctct
      121 catcaccggc gcttcttatg gaattggatt tgctattgcc aaatcctacg ctgaagccgg
      181 cgctactatt gtctttaacg atatcaatca agatctggtc aataaaggga ttgaagctta
      241 tcgtgaagtt ggcatcgaag cccatggata tgtctgtgac gtgacagacg aggacggtat
      301 ccaagccatg gtcaagcaaa tcgaacaaga ggttggtgtc attgacatcc tcgttaataa
      361 cgctggtatt atccgccgag ttccaatgtg cgaaatgagc gccgctgatt tccgtaaggt
      421 catcgatatt gacttaaacg caccatttat cgtttcaaag gcagttattc cttctatgat
      481 aaagaaaggg catggaaaga ttatcaatat ttgttcgatg atgagcgaac tgggacgtga
      541 aacagttagc gcttatgctg ctgctaaagg gggcttgaaa atgttgaccc gcaacattgc
      601 gtctgaatac ggtggagcca atatccaatg taacggaatt ggaccgggtt atattgccac
      661 tcctcaaaca gcacctcttc gtgagttgca agaagatggt tctcgccacc catttgacca
      721 gttcatcatt gcaaaaacac ctgctgcacg ttggggaaat cctgaagatt tgatgggccc
      781 tgctgtcttt ctcgctagtg atgccagcaa ttttgtcaat ggccacatcc tatatgtaga
      841 tggcggtatc ttagcctaca tcggaaaaca acctgagtaa aaatagaaag aagatcttat
      901 gaaaatcgca ttaatcaatg aaaatagtca agctagcaag aatcacatta tttacgatag
      961 tctaaaagaa gcgacagata aaaaaggcta ccaattattt aactatggta tgcgtggaga
     1021 agaaggagaa agtcaattaa cttatgtgca gaacggacta atggctgcca tccttttaaa
     1081 tacaaaggca gttgactttg ttgttaccgg ctgtggtacg ggtgtagggg ctatgcttgc
     1141 tttaaacagc ttccctggtg ttgtctgtgg tctagcagtg gacccaactg acgcttacct
     1201 ttattctcaa atcaatggtg gtaacgcctt gtctatccct tatgccaaag gatttggctg
     1261 gggggcagaa ctgaccctca aattgatgtt tgaacgctta tttgctgaag aaatgggcgg
     1321 tggctaccca agagaacgtg taatccctga acaacgcaac gctcgtatct taaacgaggt
     1381 gaaacaaatc acccacaatg atttgatgac catccttaaa acaatcgacc aagacttcct
     1441 caaagacacc atctctggca aatacttcca agaatacttc tttgaaaact gccaagatga
     1501 tgaagtcgct gcttatttga aagaagtatt agccaagtaa agctattcta aaccagaaag
     1561 gaactaatgg atgacgaaaa tattactgtt tggcgaacca ttaattcgaa tctcaccatt
     1621 agatgccacc agtatcggcg atcatgttgc cagttcgact tattttggcg gatcagaaat
     1681 taacatcgct tgtaatttgc aagccctggg tatctcaacg aaagtcttta ccgcactccc
     1741 tgccaacgag attggagatc gttttctcac attcttgaaa cagcaccaaa tcgataccag
     1801 ttcaatctgt cggcttggcg atcgaatcgg cctctactat ttggagaacg gctttggttg
     1861 tcgtcaaagt gaagttttct acgatcgtaa gcatacgagt atcagccaga ttcggccaaa
     1921 catgctagat atggattctc tctttcaggg gattagccat tttcatttta gtggaatcac
     1981 catagctatc ggtcaagagg tccgtgcgat ccttctccta ctcttggaag aagccaagcg
     2041 ccgaggaatt gtcgtttcaa tggatctcaa tctgagaaca aagatgattt cagtcctaga
     2101 agccaagtat gaattttcta agtttgcacg ttttactgac tattgcttcg gtattgatcc
     2161 tctcatgatt gatgaccaaa atctagagat gtttccaaga gacagtgcta gcctagaaga
     2221 ggtggaaaat cgcatgcgac ttttaaaaga agcctatggt ttcaaggcca ttttccatac
     2281 cctccgatct agtgatgagc aagacaaaaa tgtctatcaa gcctatgctc tggaagaacg
     2341 atttgaagag tctgtccaac taaaaactgc agtctatcaa cgaattggta gcggggatgc
     2401 ctttatatct ggtgcccttt accaactact ccatcattcc tccctaaaaa ctaccattga
     2461 ctttgcagtt gcgagcgcaa ctctcaaatg cactcttcca ggagaccatc tctccacttc
     2521 cgcaactagt attgaaaatt tactggcaaa tgcacaagat atcattcgtt aggagaatta
     2581 catgaccaaa tcagatacga ttattgaact aaaaaaacag aaaattgtcg ctgtcattcg
     2641 aggaaataca aaggaagaag gactacaagc ctcgattgct tgtatcaagg gcggtatcaa
     2701 agctattgaa atcgcctata ccaatcagta tgcaggacaa atcatcaagg aacttgtaga
     2761 cttgtatcag gacgatcaga gtgtttgtat cggtgcaggt actgtgcttg atgccgtaac
     2821 tgctagagat gccattctag ctggagcaaa ttacgttgtt tctccatctt tccatgctga
     2881 aactgcgaaa atgtgcaatc tctacagcac accgtacatt ccaggctgta ttaccctcac
     2941 agagatcacg actgcacttg aagccggtag tgaaatcatc aaactcttcc caggtagtac
     3001 tctcagtcca gcatatatct ctgcagtcaa ggcaccgatc ccacaagttt ccgtaatggt
     3061 aaccggagga gtcggcctaa acaacatccc tcaatggttc gctgctggtg cagatgccgt
     3121 tggaattggt ggcgaactca ataaactcgc ttcccaaggc aactttgacc gcatcagcga
     3181 gattgcccaa cagtatgtta cactcagata aaatcataac cacccgtcaa acgggtggtt
     3241 tatcccagag ctataagccc aaatcatcag ccaagcgcct aaagacgctg gctttcacgt
     3301 tgttcaagcc ttattgctct tgactcgtca cttgcctctt taagagactt tggtattact
     3361 taccactatc cctaaaggga tcctcatatt cttttacact caatttatct agtgctatag
     3421 tagattgaaa ctggaatagt acacatctac ttctaaaaca ttgttaaaaa tcgatttgac
     3481 tgtcctgatc gatttgttct gttcttattt cattttacta tatatcatgc tttactcgtt
     3541 ctcaaatttt catactcgtg aagaaatcat ccactggata atttctttaa tcttgactat
     3601 atttcttaat tgtggcttta ttaagcccta ctggacttac ataataacct tccgcccaga
     3661 aatgccgatt cccaaacttg tacttgagat tggcgtgttt gtcaaacatc atgagtgcac
     3721 ttttgccttt taaatacccc ataaaacttg aaacacttag cctcgacgga atactgacta
     3781 acatgtgtac atggtctggc attaagtgac cctcgatcat ttcaacacct ttataactac
     3841 acaagcgatg aaatatttcg cctaaactac ttctatattg attatagatg acttttcgtc
     3901 tatacttagg ggtgaacaca atgtgataga acacctccac tttgtgtgag ataaactatg
     3961 agccttttgt gccatatttt ttctcctttc gctttacaag tggattgaac acctttattg
     4021 tatcgcgttt ggagtttttt tggtataacc ttcgacgcgc acccgtatag cgggtggttg
     4081 ttttgtctcg cacctcacgg agcgagacgg actaatatag tggagtgaaa taggatacga
     4141 acaaattgat taggaaaatc aaatgaattt atagaaatct tttagcagtt atgttgtcct
     4201 attctagttt caaaacgcta tagaagcagc attgtgctag tctagattca gtttactata
     4261 ctaaaacgag tagcttgaaa tcaaaaaacc caccctcaca ggcaggtttt atctgtatta
     4321 ttcagctaga ttatgcttta ccttctgaac cgaatacgtc gatacgttct tcaaccgatg
     4381 cttggatagc ttttacaccg tcagccaaga atttacgtgg gtcgaagagt tttttcttgt
     4441 cgtattctgc ttcgtttgct tcgtagtcac gagcaaattt acgagttgcg ttagcgaatg
     4501 cgatttggca ttctgtgtta acgttaactt tggcaacacc aagtttgata gctgcttgga
     4561 tttgctcatc aggaatacct gatccaccgt gcaatacgat tgggaatcct ggaagagctt
     4621 ctgtcaattt ttgcaagtgg tcaaggtcaa gaccttccca gtttactggg taagggccgt
     4681 ggatgttacc gataccagct gccaagaagt cgataccagt ttcaaccatt gctttagcgt
     4741 cttcgattgg agccaattca cctttaccga tgattccgtc ttcttcacca ccgatagtac
     4801 caacttcagc ttctactgag atacctttag cgtgtgcttt ttcaacaact tctttagcca
     4861 atttaaggtt ttcttcaact ggaaggtgtg aaccgtcaaa catgattgaa gtataaccaa
     4921 cttcgataca ctcaagtgca tcttcgtagt gaccgtggtc aaggtggata gctactggta
     4981 cagtgatacc cattgattca acaaggttag cgatcaagtt gcgagcaact ttgtaaccac
     5041 ccatgtattt agcagcaccc attgaagttt ggatcaaaac tggagctttt ttagcttctg
     5101 ctgcgcgcaa gatagcttga gtccactcaa ggttgtttgt gttaaatcca ccaactgcat
     5161 aaccgttgtc acgggctgct tggacaaatt tttctgctga aacgattgcc attttatcag
     5221 gcctcctgta tatttttatg ggtcatccca tttacattgt tcattttatc actttttgcc
     5281 aaaaaaatct agtttttccc gcagtttcga ttgattttct tctaactcca tctatgtaaa
     5341 ccctttctct ccctagtctt ggacgacttt tggaaaatct ataaagaagg ttaaactatt
     5401 ctcctccatc tcgaaacgat aagctaattt ttcatgttct aatagactct taaccacaaa
     5461 gagccccata ccagacccct tgaccttgcg actggcattg tcagaaaaag actgggctag
     5521 tttttcttgt tcctctgagc tacagctatt ttcgataaaa agttctcctt ctctttctcc
     5581 aattcgaact aagccacctg gaacagagtg cttaatggca ttgctgatga gattagaaag
     5641 aatcaacttc ataactgatg ggtttagata agcctgctga tgggtcaaac tattgtctat
     5701 ctggagctct ctttccttgg ctagcaaggc ataatctttg accagatttt gcgtcatctg
     5761 gaggaggtca attgtttccc tatcatctcg caattcctgc acagaagaga gggaaagtat
     5821 ctgcagaaca tggtgattga gttcatccac aatccccaag gcaactccca gatactggtc
     5881 tctatcctta taacgaccga tattctctct catattttcg attaggattt tcaaactagc
     5941 cagcggtgtt ttcaattcat gagaagctcc tcgtaggaat tcgaccttca tcttctccag
     6001 ctggagaatg gcttcattct tttcatgcaa gtccgcaata acagtcaaga gatgctggta
     6061 gaggctattg atttgttcct tgagattacc tatctcatcc ttagaatcca cgcgcaatcg
     6121 cacttgggaa tccaggtcca tcatccgacg ggtcacccgc ttgatttcca aaatcggtgc
     6181 aacaatagtc cgagcgtaga tgtaggccac caaaagggaa atcagaaagg aggccagcaa
     6241 ggtataggga agaaactgga gactgatttg ctccgcttcc ttttgtaaat ccatggaagc
     6301 tagaaactgg agaatcatag taccaccgtc ttgcgttttc acctcgcgct cctcaataaa
     6361 gagagaggtt gtctggcggt ctgtgtccag aggaagactg tccttgactt ctaacttgtc
     6421 ctcggtcatc tcacctttga cggtcccctt gatatcacta gtctgggaat acaagtctaa
     6481 cacttgctcg atactctgcc tatctttccc ttctagggac tgggcaatgg ctgttgcctt
     6541 ttgaccaatg gtttcctgac gatgactcag ataagtcgaa ggaaaaagaa aataaatagc
     6601 taaatgaagg cagataacca gaacactaaa tatcgagaag gtatagataa atatctttgc
     6661 aaataaacct gttcgtttca ttttcgctcc aatttataac caacattgcg cacagtgagg
     6721 atacaatcca aatctagctt tttccgcaat tccttgatat aaacatcaat aacacggtca
     6781 aagggaacct catctgtcgc tttccagacg gcatcgataa tctgagatcg agtcaaggcc
     6841 cggccttcat ttttcactag atagtccaga atttccaact ctttggcatt gataggcact
     6901 tcttgacctg cgaggcttgc actgtagctt tcaaagtcca ccttggtatc cttgtaagaa
     6961 aagattcgtc ctgtatcgta gtagcgcttg aaaatcgcgt ccaccctcac ttttaaaagg
     7021 gagagggaga aaggtttttc cagatagcca tctgccaaag aggcaaaggc actcatcttg
     7081 tattcctcat cttgaaaagc tgtcaacatc aagacaggaa cctgactggt tttacgaatc
     7141 tcagctagga cttctaagcc gttgagcttg ggcatctgga tatccagtaa aaccagggcc
     7201 acctcatagc tagaaaattg ctccagagct tcctgaccgt ccgctgcctc aatagtttca
     7261 tagccacaat ccgtcaaata atcactgacc ccctcacgga tcatctcttc atcttctaca
     7321 attaaaattt tcatacttta actgctctct attttttatt tttcttagaa taaataccta
     7381 ccctattttc tattatagtc tcttgctggc cttttgtctg caagcaactg accactagat
     7441 aaaacgttgt gaaattcctt tctcataaat tccataactt tagtatatta tatttaagca
     7501 ctaaagtaca aagaaagcaa ctgaaagcaa tgattttcac cactgctttc ggatttattt
     7561 tgaattgtta aatagccatt cctatccact attcttgaat agaaacacaa gatgcaatct
     7621 ttattctaga ctcatttttt caaatttatt caccatccag caagagctct tttggttgtt
     7681 ttctaaggag attgcttgaa gcaagcgcca taacgagaac cactagaacc aaggcaagga
     7741 caaaaatgat gataaagtct gatgtctgaa tggaaatgtc taggctcgac aaggtcttgc
     7801 taaagccatc tacttctgca ccaccaccaa ggttagaggc ttgagccgcc ttactagcct
     7861 gtttggcaac acctgaagtc acattggcaa ggacagtgtt tccaattgca cgggcagtgt
     7921 aattagctag gaagtaagca gaaactagag cagggatagc aatcaagata gattcggtga
     7981 tgaattgacc caagatactt gcctgcttga ggccgataga gaggagaatt cccacttcct
     8041 tgcgacgggc gttgatccaa aggctgagca agagggcaag gaggagaact gagaagctca
     8101 agctacccca gaagaggagg ttggccatct tgtacatacc agagatagat tgctcaagag
     8161 ctgggtagtt agaggagctc ttgacgagtg tgtagctctt ccagttgata ccactgatgc
     8221 cattcaactc tttcataaca tcatccaagt tcttgtctgc tgttacaaag aaggttgcgt
     8281 ccccataaat ggctgtgtct tctgtgtatc cataaagttt tgcagcagtg tgaatgtctg
     8341 taatagctgt gttttcgtaa agttcttgtg agtaggttac tgctgactta ttatgaccat
     8401 caaagagtcc cttgattgtc acttcaactg tttccttggc tcctttttca ttatctgcat
     8461 cgtagatatt agagtccagt ttaaccttgt cccctacttt ccagccgtgt ttggctgcca
     8521 agtccttgtg caagaggatt ttatccttgt cgtcgttggt taagtgctct ccttcgacta
     8581 gtttataaga accagagaca aacttgtctt ctttagagga gtcattgaca cctgtaatca
     8641 tcaagctact tccaaaacgc ttggcacgat cagcagtgag attcttcttg gtttctggcg
     8701 tttcaatcag gtcatatcca gtcaaatctc cgatagcgtt gatacgtttg acataagact
     8761 caatggcctt gttttcggtg atttttttga tgtcttcacc cttgatattc ccagcaccac
     8821 gaggcgttcc ttggttgacg cgacgattga tttgcatgga gaagctattg gtgatatttt
     8881 taaaggtctc ctgagaagcc ttggcagtag ctcccttgat tgacaagccg accaaactca
     8941 agctcgccat gaggagaata atcaggaaga tgacaatcga tttgaaaaac ttccttgtaa
     9001 cataggcaaa tgcgttgtgt aacatagatt ccctttctag attttgtttt aatcattcta
     9061 ttaaaataag ctcaaattat ttactagtat tgcgcgtttc agtcagtttc ttatccttta
     9121 attcaagtgt aatatctgac gcttgtgcca cttctttact gtgagttacg acaatcacac
     9181 atttacctgt tttctgggca agtgatttga gtagttcgac aatatctcca gcagttttag
     9241 gatccagatt tcctgttggc tcatcagcta gaataactgg agcttctgag accaaactgc
     9301 gagcaatggc aacacgttgc tgttgaccac ctgataactg gagaacattc cgcttgatct
     9361 ggctttcatc caaaccaagc tcaagaagtg tatccttgct tgcctttttg ttgaccaagc
     9421 ggatattttc cagcggagaa agataatcta tcaagttata attttgaaag accagggaaa
     9481 tatggtgcat gcgatggtaa gaatagccct tcttacgaat atcctctcct tgaaaaagga
     9541 tagaaccttc aacaggacta tctagaccag caagtaggga caagagtgtg gattttcctg
     9601 ctcctgactc cccaataata ctgtaaaatt ttccgggttc aaaattataa tcgatctgat
     9661 ataggactgc ttcagcagta ttcttataac ggtaggtaac atcttgtaat tgtaataaag
     9721 tcatgatttc tccttcttaa ctaatagatg ataaaatttc tttcggtgat tttctaaata
     9781 agaataggaa acaaagggct acagataagc aactaagcag aactagaaaa acataggatt
     9841 ctgcaaaaga taagatgcta gttgataaac tgcttgcttt ggctagtgta tcttgtaagc
     9901 ttgcctgatc tccacttgct agtagagttt ggagtaggta agttgtgatt ccgtttcctg
     9961 caacaaatgc tggaagcaaa gctccaagag ataccaaaac tacctctaaa cagaattgta
    10021 ggaagatcga gctcttgcct tttccaagtg caagtaaaat ccccacttca tagacccgtt
    10081 ctctcaacca gagagacaaa accagaatta aggctccagc tcctgctatc aacatcccat
    10141 aaaggaagat ggtcaggaag gtttggaaag ttgcaactga gtctttgatt tgttcaaaag
    10201 ccttgttttc cttttcgact tggtagcctt gattttccaa ggccaagttt tctacctgct
    10261 tcatgagtcc gtccatttcc ttaggatttt ctacatagaa gcgtgctgca ctgacttgag
    10321 cttcactatt gcccaaaagg gtttggctac tttcatagtc tgtaaagact tgattttcac
    10381 tgaagtcaga agacaagcct gtgaatttct cttgtttttt accagaaaag atgccaataa
    10441 tctcaaactc tactgtttgt ccttttccag attcagactg accagcatcc aagccaatct
    10501 tgtcatgaag tgaaagaccg ttcttcttag ccaattcttc gtggataagg attttcttgg
    10561 aatccccttt ttgaaggtgt cgcccttctt ttagattgaa agccgaactg gtaaaggtta
    10621 catccttgga tgaatcctca agagccgtta agctaaccaa gttattgtct gcagctgata
    10681 aatcatcacg ctccacgctc tgctcgccag tcactgcttc cttgtctttt agttttgcga
    10741 ccgtctcaag ttcaggagag acattttcca gccccttaat cttgcttaca gatgctaggt
    10801 ctgacaactt gaatgtctga ccattctcta tcttcttaat agaaaaagat gtattgagtg
    10861 atttataaag attgctttct actgttttgt tggacttcat cagagtcaaa caggctgaaa
    10921 ttccggccaa taagaccaat aaaatcagaa ataaaataaa acttctcagt cgctttctgc
    10981 tgacataagc ccaagatctt tggattggat tcatttgtca cctccatatt tgtaagacta
    11041 ttataaaacc caaatatgaa atgtttatga aatacgaaaa aaaaaa
//
LOCUS       DAVMEI010000056        10618 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVMEI010000056 DAVMEI010000000
VERSION     DAVMEI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.056"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            32..313
                     /locus_tag="VYQ24_001124"
     CDS             32..313
                     /locus_tag="VYQ24_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1968270.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            445..1389
                     /gene="manA"
                     /locus_tag="VYQ24_001125"
     CDS             445..1389
                     /gene="manA"
                     /locus_tag="VYQ24_001125"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1968271.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1411..2886)
                     /locus_tag="VYQ24_001126"
     CDS             complement(1411..2886)
                     /locus_tag="VYQ24_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV1968272.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFQARSPFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVGNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMNFLVPNLLMPFGALYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVPSFQSSSWSSSPNLCNQKGLE"
     gene            3052..3216
                     /locus_tag="VYQ24_001127"
     CDS             3052..3216
                     /locus_tag="VYQ24_001127"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1968273.1"
                     /translation="MVPLKSENGYRTYSQEDLERLQVILYYKYLGFSLEKIAELLKEE
                     RTDLLLHLTR"
     gene            3217..3723
                     /locus_tag="VYQ24_001128"
     CDS             3217..3723
                     /locus_tag="VYQ24_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEV1968274.1"
                     /translation="MDYLTRERQHLDTLISTLQKTIQEQKGERKMTIEEKFTGFSYQD
                     NQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAFNQVFQTLAQNLQVGLPATATENQ
                     EQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEFKENIDKFGSETAQYTSDAIAAYV
                     QTNAEKIG"
     gene            complement(3743..4198)
                     /locus_tag="VYQ24_001129"
     CDS             complement(3743..4198)
                     /locus_tag="VYQ24_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1968275.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4218..5393)
                     /locus_tag="VYQ24_001130"
     CDS             complement(4218..5393)
                     /locus_tag="VYQ24_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1968276.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5452..6297)
                     /locus_tag="VYQ24_001131"
     CDS             complement(5452..6297)
                     /locus_tag="VYQ24_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1968277.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6422..6979
                     /locus_tag="VYQ24_001132"
     CDS             6422..6979
                     /locus_tag="VYQ24_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1968278.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            6998..7465
                     /locus_tag="VYQ24_001133"
     CDS             6998..7465
                     /locus_tag="VYQ24_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1968279.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7554..8183
                     /gene="upp"
                     /locus_tag="VYQ24_001134"
     CDS             7554..8183
                     /gene="upp"
                     /locus_tag="VYQ24_001134"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1968280.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFIVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8357..8947
                     /gene="clpP"
                     /locus_tag="VYQ24_001135"
     CDS             8357..8947
                     /gene="clpP"
                     /locus_tag="VYQ24_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1968281.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9026..9274
                     /locus_tag="VYQ24_001136"
     CDS             9026..9274
                     /locus_tag="VYQ24_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1968282.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9375..10535
                     /locus_tag="VYQ24_001137"
     CDS             9375..10535
                     /locus_tag="VYQ24_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1968283.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
BASE COUNT         3359 a         2104 c         2273 g         2882 t
ORIGIN      
        1 tagtattatc gtagaaaatg gactcggtgc tatggataca ccggatgaga atggttatgt
       61 agcagatgac tatcggatta cttacttaga ggcccacatc aaggccatgc gagatgccat
      121 ttacaaagac ggggttgact tgcttggtta tacgacatgg ggctgtatcg attcggtttc
      181 agctggcaca ggggaaatga ataagcgcta tggctttatc tatgtagatc gagataatgt
      241 aggaaatggg actctcaaac gtagcaagaa gaagtccttc tattggtaca tgtcatttat
      301 agcaatggtg taagcatcga gtagagcaca tttgtttact atttttataa aatcttctcc
      361 ctgctttata agatgtaaaa aagagttcaa ttcgaactct ttttttgcta taatgagggg
      421 agaaaaatca gacaggagat taagatgtca gaaccattat ttttacaatc agttatgcaa
      481 gaaaaaatct ggggtggtac caagctacgt gatgagtttg gctacgacat tccaagtgaa
      541 aaagtcggag agtactgggc tatctcagct catctaaatg gtgtttctaa agttgccaat
      601 ggtcgttacg agggaacaga tcttgctact ttgtatgcgg aacaccgtga attatttggc
      661 aatcgtcctg agcctgtatt tccgcttttg actaagattt tggatgctaa cgactggctc
      721 agcgttcaag ttcacccaga cgatgcttat ggactagagc atgaaggcga acttggaaaa
      781 acagaatgct ggtacatcat cgctgcggat gaaggttcag agattatcta cggccacaat
      841 gccaagtcaa aagaagaact ccgccagcaa atcgaggata agaactggga tgcgctgttg
      901 acaaaagtgc cagttaaagc aggagatttc ttctatgtac caagcggtac catgcacgct
      961 atcggggcgg gtatcttgat tcttgaaacc cagcagtcta gcgataccac ttaccgtgtc
     1021 tatgactttg accgtaagga tgacaagggc aatttacgtg aacttcacct tgaaaaatct
     1081 atcgatgtct tgaacattgg tgaacccgca aatagccgtc cagtgacaat caaagcagat
     1141 gatctacgtt ccactctcct tgtatccaat gatttcttcg cagtttacaa atgggaaatt
     1201 actggaaaag ttaactttga aaagacagct gactacagct tgttgagtgt cttggctggt
     1261 caaggtcaac taactgttga cgggaaaaac tatccaatcc aaaaagggag acactttatc
     1321 ctaccaagtg atgttgaagc ttggactctg gaagggcaag atttagaatt aattgttagt
     1381 catccataaa aaagaaaggg cctgagttca ttactcaagt cctttttgat tacataaatt
     1441 gggcgatgaa gaccacgatg atgattggaa tgacggaacg aagaaacgaa gaaggaagag
     1501 ccagacttgg aacagtcctt gtttccatgc tctttcatcg agatggagtt cctccattgc
     1561 aagagccttt ttaaagatat agcctgtaaa aagtgaaagg tagagagctc caaatggcat
     1621 gaggagattg ggaaccaaga agttcatagc gtcaaagaag gtcttaccaa aaatgtgaac
     1681 atccgccatg acaccgtaag atagggctga aggaatgcca aagacaaagg tcaaaattcc
     1741 taaaataaca ctccatttgg cacgtttgct gttatcctgg ttggtgatat tgcccacatt
     1801 gatctccagc atcacgacag aagaagtgac agtcgcaaag aggaagagga cgtagaaaat
     1861 ggttccaaaa ggcatcttgt caaagagttg aggcaagacg ataaagagca agctcggtcc
     1921 cccttcagac tggatattga agggggaccg agcttggaaa atggctagac ctgccatgat
     1981 ggatatcgag atattcatgg ctacgatgga gattcctgac tggactagat tggttttctt
     2041 gtccaagtaa gaagcatagg tcaacatgac tgtaacccct agtgagaggg caaagaaaga
     2101 ttgtcccaga gcatagagga gaccagcgct agtcagtttt gaaaagtctg gtttgaggaa
     2161 gtaaagaacc ccttccatgg catttggcaa actgagagag cgaccgatga taacaacaaa
     2221 gatgataaag agcaggggca tcatgacttt cgaagctctt tcaatccctt tttgaacccc
     2281 acgtgataca ataaagatat ttaataagat aaaggctgct tgagctccta gggcaatggc
     2341 tggatttgaa atgattgaag taaataactg agcataatca cccgttccac caagttggaa
     2401 caatttccca aactcaatac ctagatagac tagaatccat cctccgataa cactgtaaaa
     2461 agataagagg ataaagaggg caaaggcgcc aatccaaccg ataaagttgt acttgttatt
     2521 cttgcccagt tttccaaagg ttttgatagc ggaaacgcca gcactacggc caagggcaaa
     2581 ctcagccagc aggagaggga aaccgattaa aatagtggaa atgagaaaga ttagtaaaaa
     2641 gcctccaccg ccattagcag cagtcatgta gggaaacttc caaacggaac caagcccgat
     2701 gaccgagcca gcagatgcta gaataaagcc aagtttcgac ccccattgtg atttttctga
     2761 catagttaaa ctctgatctc ctttttcaaa ataagaaaaa gctacttgag ttgtcaaata
     2821 gccttctgtt tgattgatag acttgactat cctattatgt tttctaaggt ctgtcaagaa
     2881 aaccattttc aagttttcac acctttctca aaaacgcttg actctgacct aaggggaggg
     2941 gttatactat cattgtaagg aggaaatcat gtaccatata aaagaagctg cgcagctttc
     3001 aggtgtctct gtcaagaccc tgccctgcat cactatgaca agataggact cttggtcccc
     3061 ttaaagtcgg aaaacggcta tcgaacctat agtcaagagg atttggaacg ccttcaggtc
     3121 attctttact acaaatatct aggcttttct ttagagaaaa tagcagaact gttaaaggaa
     3181 gaaaggacag atttattgct ccatttgact aggtagttgg actatctaac tcgcgaaagg
     3241 caacatctgg ataccttgat ttccaccttg caaaaaacta ttcaagaaca aaaaggagaa
     3301 agaaaaatga ccattgagga aaaattcacg ggatttagct atcaagacaa tcaaaaatac
     3361 caccaagaag cggtagagaa atatggtcaa gaagtcatgg gacaagcgct cgaacgccaa
     3421 aaaggtcacg aagacgaggc tacggccgcc tttaaccaag tctttcaaac tttggcacaa
     3481 aatcttcaag ttggtttacc tgcaacagca accgaaaacc aggagcaagc agccaagctc
     3541 ttgcaagcca ttcgcactta tggatttgac tgctctattg aggtattcgg tcatatcggt
     3601 aaaggttacg tctacaaccc agagtttaag gaaaacattg acaagtttgg ttctgaaaca
     3661 gcccagtaca cgtcagatgc cattgcggct tacgttcaga caaatgcaga aaaaataggc
     3721 taggaatttc ctagcctatt ttttacttca aatcataaag ccagtcgtca ccgtttttgt
     3781 agtaaaagaa ttcactgaga tcttcttcta gaaacacacg aagcatatca gacatatcat
     3841 cggttgcaag ttttagatga gaaagatttt caaagtcctc ccaccaaact ttcccttcgt
     3901 ctgaagactg gagttcacca gtaaagtgtt ctgtcttgta aaaaaggacg acataacgat
     3961 aatccttgtc gtcataccag tttttgatac cacagagttg gggtttggaa atgatcagac
     4021 cagtttcttc tttcacttca cgaatgacag catcgacaaa ggattcgcca cgttcaacat
     4081 gaccaccagg aaaagtaatg ccagaccagt cgggactaac tcggtcttgg accaggacct
     4141 tatctccgtt tttaatcata cacatgttaa caaattcgac tgcctctctt ctgttcattc
     4201 ttcacaacct ttaatcttta atcataatgc agacttcccg ccacccagcc ggtacagagg
     4261 gcagaagtga tgttaaagcc acccgtgtgg gcattgatat ccataacttc gcctgcaaag
     4321 tggaggccag gtaccagctt actttcaagg gttttaggat tgatttcctt gagactgact
     4381 ccacccttgg taacaaagga ctttgcaagg gacatttttc cagttacagg aattttaagt
     4441 tctttaatgg actggacaag ttgttctcgt tccttttcag tcagttgttt gactttttca
     4501 ggatatcctt gtacaaaaaa ttcggccaag cgttctggta acaaggtttt taaagcgttt
     4561 ttcaaggatt tttcccgatt ttcttctaga aatgtaacca agtccttctc agaaagttga
     4621 ggcaaaacat cgagtgagag aacctcccca cctttgacaa agctagacat gcgtagggca
     4681 gcaggacctg acaaaccaaa gtgggtaaag agtaaatcat gagtgatgac atgcttacca
     4741 taacttaggg tcacatcgtc cagagaaatc ccttgtaagg ctttatgtgg aaaatctgtt
     4801 aataaaggac tttcagcagc ctcaagatcg gtgatggtat gcttaaaatg gcgagcaatc
     4861 tcgtgaccaa aaccagtcga accagtcgaa ggataagact taccacctgt tgtgacaatg
     4921 agtttctcac aagtgaaggt ttgatccgct gacttaagga caaactggtc atctactttt
     4981 ttaacagaaa cgatttctat ttgagtagca acttgaccac ctagttcggt gattttcttt
     5041 tccaaagctt cgataatagt ccgagacttg tcactggctg gaaagacgcg tccgtggtct
     5101 tcgaccttaa gtttaacacc attttctgta aaaaagttga tgatgtcatg attatcgaac
     5161 tgggagaaaa cactgtaaag aaagcgtccg tttccaggaa ttccagctag caggttgtct
     5221 aagctaccat tgttggtcac attgcaacgt cccccaccag tcccagctaa tttttttcca
     5281 agtttccgat ttttttcgat gaggagggtt ttctgtccat aaaagctact ggaaatcgta
     5341 gccatcatac cagcaggtcc cccaccgatg acaatagtat caaaatgttt catagctcta
     5401 ttgtaccaca aaaaaacaag agatgatggt cacctcttgt caagaatgca attaatcaat
     5461 ttcatagccc atcagcaaac cgccctcttc tgcatagaaa ctgcagagac cagaggttgg
     5521 tagaatttta atatccgctt gtgggaaggt ttcacggatt cgctctgaga gctgttgaca
     5581 acatttttcg ttattgcgtt gggccatgac aatacggcca ccagcatatc cagcttttac
     5641 taactcatca taggcagctt gaactgattt ctttgatccc cttgcttttt gtagcaattc
     5701 gagagttcca gtttcactag cttttccgac catacgaatg ttgagaaggc caacgaccgt
     5761 accgataagc ttgctcaaac ggccgttctt caccaagtta tcgactttgg ctaggacaaa
     5821 gagcaactta gttttttctt gataggcagt gatagcttca accacttctt caaaagacaa
     5881 gccctggtca atcaagtcat tcaatttttc tacgagtagg tcaacttcac caccagcaga
     5941 taaactatca atcacatgaa tcttagtgtc aggatggtct tctagataaa tattctttgc
     6001 tagttgagca ctattgtgac tgccagaaag ggtacctgtg atggttacta ggaaaatgtt
     6061 tttggcacct tcaaatgctc gcaaatagtc atctgggctt ggacaagccg attttgaagc
     6121 ttctgcagtt gcatacatgg tttccatcat ttggtcaata tcgagactgg cgtcatcaac
     6181 aaagacctga tcagctactt gaatggttaa ggggacactt acaaaggttg tgttaatagc
     6241 tggtgttggc agttgacgat aatcacaacc agagtcagca ataattttcc aagtcataga
     6301 aattctccat ctttgtcagt tatacattga caaaggttct gtcttttttt acaattatat
     6361 catgaaaacc cttgaaacaa aagcatcatc cgtctgttgt gacaagtaga aagaaaatgt
     6421 tatgtctgaa cgtagaatct ctgaaaagtc tcttgaaaat ctcagaaaat caaaccaaga
     6481 atccaattta ttaaccagag aagccattga aacagccctc ttgcaactct tggaaaaaaa
     6541 ggagctgacc aagattagta tttctgaatt ggtcaaacgt gcaggtgttt cgcgtgcggc
     6601 cttttatcgc aattatgact ccaaagagga aattttagag agcgtcttta aacgaactgt
     6661 ccacaatatt atggaacagc tgcatcatta cgatttaaag acagaccttt atttggtttg
     6721 ggttcacctt ttccgggagg ccagaaagga agctagagtg attcaattgg ccttggatta
     6781 ccatctggaa aaaatctttg tccaagccat gcaggaattt ctagaaaaat accatgggaa
     6841 atcaaaaggt gtcagctctt atcttcattc cttctggagc tcagccatcg tctctgtcct
     6901 tctaaaatgg atcaaggatg gcatgaaggt accagctgaa aagattgcag atttaggttt
     6961 accatttttt aaaaaataga gaaaaaggag aaaagagatg actgaaaaaa gactggcctg
     7021 ggatgagtat tttgcagccc aagccctact aattgcgaat cgttccactt gtaaacgtgc
     7081 taaagtgggc gcgattctgg taaaagataa taaggttatt tccactggtt acaatggttc
     7141 ggtgtcaggg actgagcatt gtattgatca cgaatgtttg gtcattgaag gccactgtgt
     7201 tcgcaccctt cacgctgagg tcaatgctat ccttcaaggt gctgagcgtg gtgttcccaa
     7261 aggctttaca gcctatgtaa cccattttcc ttgtctcaac tgtacaaaac aattgcttca
     7321 ggtcggctgc aagcgcgtgg tttatatcaa ccagtaccga atggacgact acgcccaata
     7381 cctttatcaa gaaaaaggga cagaattgac ccatttacca cttgagacag tacagacagc
     7441 tcttaaagag gctgatctaa tgtaaaaatt atcaaaaata aatggcttag aaagattttt
     7501 aaaccgtttt ttggtataat aagaagaata aattgaaaga aggaattcca aaaatgggaa
     7561 aaattgaagt tattaatcac ccactgattc aacacaaatt gtcaatcttg cgtcgtacag
     7621 atacttctac aaaagctttt cgtgagctag tagatgagat tgcaatgttg atggggtatg
     7681 aagtacttcg tgatcttcca ctagaagatg tggaaatcga aacaccaatt acaaaaacag
     7741 ttcaaaaaca attggcaggt aagaaattgg ccatcgtccc aatcttgcgt gcaggtatcg
     7801 ggatggttga tggtctcttg agcttggttc cagctgctaa agttggccac atcggtatgt
     7861 accgtgatga agaaacactt caaccagttg aatacttggt gaaattgcct gaggacattg
     7921 accaacgtca aatttttata gtagacccaa tgttggcaac aggtggctca gcaatcttgg
     7981 ctgttgattc tcttaaaaaa cgtggcgcat caaatatcaa atttgtctgc cttgtatctg
     8041 ctccagaggg tgtaaaagcc cttcaagaag ctcatccaga tgtagaaatc tttacagcag
     8101 ccttggatga acgtttgaac gaacacggtt atatcgttcc aggtcttgga gatgctggag
     8161 accgcttgtt cggtacaaaa taagatcgaa aagaagaatg acttggaaaa gtatttccag
     8221 tcacgaaagg aggttgggtt tttgtttctg tctaatgaaa gcagagcaaa aatttgacct
     8281 tttttgacca agatattata atagtcttat cttcagtcat ttgaccaaca aaattaaaac
     8341 tcaaaaggag aaatgaatga ttcctgtagt tattgaacaa acaagccgtg gagaacgttc
     8401 ttacgatatt tactcacgtc ttctcaaaga ccgcatcatt atgctgacag gtccggttga
     8461 agacaatatg gctaactctg ttattgccca attgcttttc ttggatgccc aagatagtac
     8521 aaaagatatt tacctttatg tcaatacacc aggtggttct gtttcagctg gtttggcaat
     8581 cgtagatacc atgaacttta tcaaggcaga tgtccaaacc attgttatgg gaatggctgc
     8641 atctatggga acagtcatcg catcaagtgg agcaaaaggc aaacgtttca tgcttccaaa
     8701 tgctgaatac atgattcacc aaccaatggg cggtacaggt ggtggtaccc aacaaactga
     8761 tatggctatc gctgcagaac acttgctcaa aactcgtaat accttggaaa aaatcttggc
     8821 tgaaaattca ggtcagtcaa tggaaaaagt ccatgcagat gcagaacgtg ataactggat
     8881 gagcgcccag gaaacacttg aatatggctt tattgatgaa attatggcca acaattcatt
     8941 gaactaatga tgatagaagg caaactcgac tgggtttgct ttttttggta taatagggag
     9001 agatttctta gaaagaggat ttatcatgtt tgaaaaagtc aatcgctctg gcttgattat
     9061 ctatctttac tataatcgtg atgccaaaaa actgcaggat tatggagata ttacctatca
     9121 ttccaagaaa catcgttact tacaactcta tgttccaact caagaagtgg agcaattggt
     9181 cggacgcttg agcaaggaaa aatttattaa aaaagttagg gtttgtcata tccaagagtt
     9241 ggaaacaccc tttgtgggca atctttatcg ataggaaaac gttatcatcg aaaaagttca
     9301 aggaaaacat tgacaatttt ctgataattc ggtatattct taacagacta tttaagaaat
     9361 aaggagacaa aaagatgaag aaaaaatttg ccctatcgtt tgtggcgctt gcaagtgtag
     9421 cacttcttgc agcctgtgga gaagtgaagt ctggagcagt caacactgct ggtaactcag
     9481 tagaggaaaa gacaattaaa atcgggttta actttgaaga atcaggttct ttagctgcat
     9541 acggaacagc tgaacaaaaa ggtgcccaat tggctgttga tgaaatcaat gccgcaggtg
     9601 gtatcgatgg aaaacaaatc gaagtagtcg ataaagataa taagtctgaa acagctgagg
     9661 ctgcttcagt tacaactaac cttgtaaccc aatctaaagt atcagcagtc gtaggacctg
     9721 caacatctgg tgcgactgca gctgcggtag cgaacgctac aaaagcaggt gttcccttga
     9781 tctcaccaag tgcgactcaa gatggattga ctaaaggtca agattacctc tttattggaa
     9841 ctttccaaga tagcttccaa ggaaaaatta tctcaaacta tgtttctgaa aaattaaatg
     9901 ctaagaaagt tgttctttac actgacaatg ccagtgacta tgctaaaggg attgccaaat
     9961 ctttccgcga gtcatacaag ggtgaaatcg ttgcagatga aactttcgta gcaggtgaca
    10021 cagacttcca agcagccctt acaaaaatga aagggaaaga ctttgatgct atcgttgttc
    10081 ctggttacta taatgaggct ggtaagattg taaaccaagc gcgtggtatg ggaattgaca
    10141 aaccaatcgt tggtggtgat ggattcaacg gtgaggagtt tgtacaacaa gcaactgctg
    10201 aaaaagcatc aaacatctac tttatctcag gcttctcaac tactgtagaa gtttcagcta
    10261 aagctaaagc cttccttgac gcttaccgtg ctaagtacaa tgaagagcct tcaacatttg
    10321 cagccttggc ttatgattca gttcaccttg tagcaaacgc agcaaaaggt gctaaaaatt
    10381 caggtgaaat caaggataac cttgctaaaa caaaagattt tgaaggtgta actggtcaaa
    10441 caagcttcga tgcagaccac aacacagtca aaactgctta catgatgacc atgaacaatg
    10501 gtaaagttga agcagcagaa gttgtaaaac cataatagaa aaatgttgaa atagggaatg
    10561 agcctttgac tcactccctg tttcgatatt taatactctt cgaaaatctc ttcaaact
//
LOCUS       DAVMEI010000057        10599 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVMEI010000057 DAVMEI010000000
VERSION     DAVMEI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.057"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(258..1505)
                     /locus_tag="VYQ24_001138"
     CDS             complement(258..1505)
                     /locus_tag="VYQ24_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1968284.1"
                     /translation="MSHNDSILNILGIKDKNIKIISVEEAEHNNDSVKEYITLITATL
                     SYPINRCRNCGFPTVNKDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITN
                     LTKENQTLSSDLKNQIMLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLP
                     KHLCFDEFRSIKSVMSFICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSV
                     VIDLNAQYQSFIYRLFPNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMK
                     SHWKLFHKKAEDLHPEEVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKAL
                     NERNSELLESTILDYQKTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKT
                     LKRTCYGFANQKFFFLRIDCIFS"
     gene            1685..1906
                     /locus_tag="VYQ24_001139"
     CDS             1685..1906
                     /locus_tag="VYQ24_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968285.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLEIVVIGLYLGFKRKFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            2023..2520
                     /locus_tag="VYQ24_001140"
     CDS             2023..2520
                     /locus_tag="VYQ24_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEV1968286.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            2495..3001
                     /locus_tag="VYQ24_001141"
     CDS             2495..3001
                     /locus_tag="VYQ24_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1968287.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            2985..5432
                     /locus_tag="VYQ24_001142"
     CDS             2985..5432
                     /locus_tag="VYQ24_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1968288.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            5435..7612
                     /locus_tag="VYQ24_001143"
     CDS             5435..7612
                     /locus_tag="VYQ24_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1968289.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFIPATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            7609..8610
                     /locus_tag="VYQ24_001144"
     CDS             7609..8610
                     /locus_tag="VYQ24_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEV1968290.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            8607..9539
                     /locus_tag="VYQ24_001145"
     CDS             8607..9539
                     /locus_tag="VYQ24_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEV1968291.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            9784..9900
                     /locus_tag="VYQ24_001146"
     CDS             9784..9900
                     /locus_tag="VYQ24_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV1968292.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            9916..>10599
                     /locus_tag="VYQ24_001147"
     CDS             9916..>10599
                     /locus_tag="VYQ24_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV1968293.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGID"
BASE COUNT         3353 a         1930 c         2222 g         3094 t
ORIGIN      
        1 attctatgag tcgctttttt aaatttggaa agttacacgt tactaaaggg aatggagata
       61 aattattaga tatactactg acagcttcca agaagctaaa gaggtcccta gcgcctacgg
      121 ggaatttgta tcgataaggg gtacaaattc ccactaagcg ctcgggaccc cttgtaggaa
      181 aatgtcctaa gtgggatatc tgtcaactgg tatggttgac taaaaatact tcctacgaaa
      241 atgtaggggg tattttttta cgaaaaaata caatcgattc ttaaaaagaa aaatttttga
      301 ttggcaaaac cataacaagt tcgttttagg gttttgattt tgcgattgat gccttctaaa
      361 ggaccattag agtattcaaa tttagcgcta tttaagacat attttctgtt ttgacgaagg
      421 gtttgaatag cagtatccat ttctgtattg gttttttggt agtctaagat ggttgactct
      481 agtaattcac tattgcgctc gtttagggct ttcgtgatat cttggtaagt ttggtatact
      541 tcagcgaact tggaaaattt actagtaatg agatcaacag cattttggcg agtcatatat
      601 tgtttaacgc cgcgaagaaa aactacttct tcagggtgaa gatcttcagc ttttttatgg
      661 aatagcttcc aatgtgactt cataatttta tattcttggc tctgtttatc aagttgcttt
      721 aggatagaga tacgacaatt gtccaaagcg cgaccagcta attgtacaag gtggaagcga
      781 tcaataatga tattggcatt agggaaaagg cgatagataa aactttgata ttgagcattt
      841 aaatcaatta caactgattg aacgcattcg cgttcggctt ttgaataacg actttcaaaa
      901 taatcaacaa tggtaggtga tagacgatcc tgtaactttg tgacaatttg gtgggtttca
      961 gcgtcacaac agataaagga catcacagac ttaattgaac gaaactcgtc aaaacataga
     1021 tgcttaggca acttagccac acgatagtgt ggttccatgc gctctaagat tgttcgacga
     1081 acactgctag gagagcagtg acacatttca gcaataagct gaccagataa gcctttacga
     1141 gctaaaagca tgatttgatt tttgagatca ctggataagg tttgattttc tttggttaaa
     1201 ttagtaatag caccaaaagt agtatggcat gatttacatt tatagcgttg tttacgaagc
     1261 tctagttcat atcttctccc atttaaactt gccagtcgta catgagtttt gcgaaagcca
     1321 tccttattaa ctgtgggaaa gccacagtta cgacaacgat taatcggata agaaagagta
     1381 gctgttatta gcgttatata ctctttaaca gaatcgttgt tgtgttcagc ttcttcaaca
     1441 gaaataattt taatattttt atctttaatt ccaagaatat ttaggataga atcattatgg
     1501 gacatttgtt taaccttctt tcatgatttt tgtggtgaat tgattgtata acgaggggac
     1561 agcaaatgtc ctcttttttg tataaaaaaa tctggcatgg aatctctatc cataccagaa
     1621 agtgtatacc ccaaaaaaat aactcaaata caaattcatt gaatatagag aggagaacat
     1681 ttttatgaat tttggacaaa acctttataa ctggtttcta tcaaacgctc aatcactggt
     1741 gcttttagaa atcgttgtga ttggcttgta tcttggcttc aagcgtaagt ttagcaaact
     1801 gattggcttt ttaattattg cgattattgc ggttggctta gtcttcaacg ctgctggagt
     1861 aaaagacatt ttactagagc tattcaatcg cattattggt gcttaaataa aaccgttctt
     1921 ttgtggaata taagtggttt tcttatgttc cgcaaaggaa tggtacacca aacgaagtgc
     1981 ggtagggatt tttgaatctc tacaaagaaa ggacgtgaat atatggacga tatgcaagtc
     2041 tatattgcga atttaggcaa atacaatgag ggcgaattgg tcggtgcgtg gtttaccttt
     2101 cccattgact ttgaggaagt caaagagaaa atcggcttga atgatgaata tgaggaatac
     2161 gccattcatg actacgagtt accctttacg gttgacgaat acacttccat tggcgaactc
     2221 aatcgactat gggaaatggt atcggaatta cccgaagaat tacaatcgga gctatctgct
     2281 ctgctcactc atttttcaag cattgaagaa ctaagcgaac atcaagagga tattatcatt
     2341 cattccgatt gtgatgatat gtatgacgtg gcacgctact acattgaaga aacgggtgct
     2401 ttaggcgaag taccagctag tcttcaaaac tatattgatt atcaagccta tggtcgggat
     2461 ttagaccttt caggaacgtt tatctcaacc aatcatggga tttttgaaat cgtctattaa
     2521 atctgtcggt acattactac tggcagattt tctattttac ggggtggctc aatcagctac
     2581 ccctattttt tatgaaagga ttgattacat gaagaaaata cgaagctata ccagtatctg
     2641 gtctgtggaa aaggtactgt attctatcaa tgattttaga cttccgtttc ccataacctt
     2701 tacgcaaatg acatggtttg tcgtgtcact ctttgcagtg atgatacttg gcaacttgcc
     2761 ccctctttcc atgatagagg gagcatttct caaatacttt gggattcctg tggctttcac
     2821 atggtttatg tctacaaaaa cttttgatgg taaaaagcct tatggatttt tgaagtctgt
     2881 cattgcttat gcactgcgac caaagctgac ctatgcagga aaaaaagtaa cgcttggcag
     2941 aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca tatccaatta
     3001 aatacattga aaacaatctc gtctggaata aagacgggga atgttatgct tactatgagc
     3061 ttgttcctta caattactca tttctaagtc cagaacagaa aatacaagtg catgattctt
     3121 tcagacagct tatcgcacaa aatcgtgatg gcaaaattca tgctttacaa atcagtacag
     3181 aatccagcat acgttctgca caagagcgtt ccaaaaatga agtcactggc aagctcaaag
     3241 cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg attggcgaaa
     3301 atcaagtgaa ctaccgtttc tttatcggct ttaagttgct tctcaacgat caggagtttt
     3361 ctatgaaaag tcttaccgtt gaagcaaaaa atgctttgtc tgattttgtc tatgatgtga
     3421 accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg cgttttcaga
     3481 agatggaaaa gctcttagaa aataaaatct ctcgtcgttt caaaatccgc aggttagata
     3541 aggacgactt cggctatctg attgaacacc tttacggaca gacaggcact gcctatgaag
     3601 agtatgagta ccatctatca aagaaaaagc tggataatga aacgctgatt aaatactatg
     3661 acttgattaa gcctactcgc tgtttggtgg aagaaaaaca gcgatatttg aaaatccagc
     3721 aggaagatga aaccgtctat gtagcttact ttaccattaa cagcattgtc ggagaactgg
     3781 acttcccgtc ctctgaaatc ttctactacc agcaacagca atttacattc ccgattgata
     3841 cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc cgcaataaaa
     3901 agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa accagctcca
     3961 atgtggcgga agctctggaa agtgtgaatg agctggaaac caatttagac caaagcaagg
     4021 aatctatgta caagctgtct tatgtggtaa gggtatcagc aaatgatctt gacgaactca
     4081 aacgtcgttg taatgaagtg aaagattttt atgacgattt aagcgtaaaa ctggtacgac
     4141 catttgggga tatgctcggc ttacatgaag aatttttacc tgccagcaag cgttatatga
     4201 atgattatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt ggtgctactc
     4261 aaatgctggg ggaaaatgag gggatttatg ttggctacag cttagatact ggacgcaatg
     4321 tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc aatgcgttag
     4381 cgtcggcttt tgttggttcg ctgggtggtg gtaaatcctt tgcgaataac cttatcgtct
     4441 attatgcggt gctttatggg gcacaagcag tgattgtaga cccaaaagca gaacgtggca
     4501 gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcact ctgacttctg
     4561 atgagaaaaa caaaggctta cttgaccctt atgtgattat gaaaaatccc aaagattctg
     4621 aatcactggc tattgatatt ctgacattcc ttacggggat ttcctctcgt gatggggaac
     4681 gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagta cgagggttga
     4741 tgaaagtgat tgaggaatta cgggttgaga atacgccact aagtaccagt atagccgacc
     4801 atatcgaaag ttttacagac tatgactttg cacatttatt attcagtaat ggttatgtgg
     4861 agcagtctat cagcttagaa aaacaactga acattataca ggttgcggac ttggtacttc
     4921 ccgacaagga aacttccttt gaggaatata ccactatgga gcttttatcc gttgctatgc
     4981 tgattgtcat tagtaccttt gctttagact ttatccatac agaccgaagc attttcaaga
     5041 ttgtagattt agacgaagca tggagctttt tacaggtagc acaaggaaaa acactatcta
     5101 tgaagctggt tcgggctggt cgtgctatga acgctggggt atatttcgtg acccaaaata
     5161 cagacgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt gcatttcgtt
     5221 ccactgacct taacgagatt aaaaagacct tagccttttt tggtgtagac ccagaggacg
     5281 aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc agtgatttat
     5341 atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc catgcctttg
     5401 ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatag taaacagaat
     5461 aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg ctttcacact
     5521 tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg cgggcttggt
     5581 agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa actatcaact
     5641 ggatttttat gtggataata gctggggctg gcttccgtgg aactggtcgg acgggattgg
     5701 aaaacaggtc atgtatggac tatatgccat taccaatttt atttggacaa tcagtttgta
     5761 tgtttccaat gcgacaggtt acttagtaca ggaagcctat tccttagact tcattcccgc
     5821 tacagcagat tccattggta agaatatgca gaccttagct ggtgtgagtg caaacggatt
     5881 ttcaacagag ggtttctatg ttggattcct cttactcttg attttggttc ttggggttta
     5941 tgttgcctat acgggactga taaagagaga aaccacaaag gcaattcatg ccattatgaa
     6001 ttttgtgctg gtgtttatcc tatcggcttc ctttattgcc tacgctcccg actacattaa
     6061 aaaaatcaat gacttttcat cagacatcag taatgccagt ttatcacttg gcacgaagat
     6121 tgtcatgccc cattccgata gtcaaggcaa ggacagcgtg gacttaatca gagatagcct
     6181 gttttccata caggttcagc aaccgtggct actgcttcaa tacaacagtt cagacattga
     6241 aagtatcggt attgaccgtg tggaaagcct gctctccacc agcccagatt ccaacaatgg
     6301 cgaagacaga gaaaaaattg ttgcggaaga aattgaagac agaagcaata ccaatctaac
     6361 cattacaaag accattaacc gtttaggtac agtcttcttc ctatttgtct tcaatattgg
     6421 gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg tactttttat
     6481 catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat catttgatgg
     6541 tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag ctggaatcac
     6601 attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat cggctggtta
     6661 tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct acttcaagct
     6721 gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg tgggaagtcg
     6781 tgtgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac agcggaaact
     6841 tggacgttcc atgactactc taggggctgg gtctgccatt gttacaggta aaaaaggaca
     6901 gtcgggttcg gggagttctg caaggacaca agcagatcac tcccgaccag acggaaagga
     6961 aaaatcaaca cttggaaaac gtatcggtca aaccatcggt acagtagctg ataccaaaga
     7021 cagaatggta gacactgcta gtggtttgaa agaacaggtt aaagatttgc cgaccaatgc
     7081 aagatatgca gtatatcaag gaaaatccaa agtaaaagag aatgtccgtg atttaaccag
     7141 tagtatttct caaaccaaag cggacagagc cagtggacgc aaggaacagc aggaacaaag
     7201 gcgaaaaacc attgcgaagc gtcgctctga aatggaacag gtcaaacaga aaaaacagcc
     7261 tgcttcttct gttcatgaaa gaccgactac aagacaagaa caatatcatg atgaacagac
     7321 ctcaaaacag tctaatattc agacttcata taaggaatct caacaagcca aacaagagcg
     7381 tccagcagtt aagtccgatt tttcaagtcc aaaagtggaa cgccaaggca ataccgttca
     7441 agaaaaaacc gttcaaaagc cagcaacttc aaccactaca gcagatagaa cttcacaacg
     7501 tccaatcaca aaagaacgtc cgtctactgt tcaaagagta ccactacaaa atacaagaag
     7561 tagaccacca atcaaaaccg ccaccattaa gaaagtcggt aagaaaccat gaagttgaaa
     7621 actttagtga ttggtggttc tggattattc ttgatggtct tctcactgct tctgtttgtt
     7681 gccattttat tttcagatga acaggacagc ggaatttcca atattcatta tggaggtgtg
     7741 aatgtttccg cagaagtgct ggctcataag cctatggtag aaaaatatgc caaagaatat
     7801 ggcgttgaag aatatgtcaa catacttctt gcgattatac aggtggaatc gggcggtact
     7861 gcggaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc attgagtaca
     7921 gaagaatcca ttaagcaagg tgtgaagtat ttcagtgaat tattagccag tagcgaaagg
     7981 ctcagtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg tttcttaggg
     8041 tatgtggcta atcgtggaaa taaatatacc tttgaactgg ctcaaagttt ctcaaaagag
     8101 tattcaggtg gcgaaaaagt gtcttacccc aatcccatag ccatacctat caatgggggc
     8161 tggcgataca actatggcaa tatgttttat gtgcaactgg taacgcagta tcttgtcaca
     8221 acagagtttg atgatgatac ggtacaagcc atcatggacg aagcactgaa atatgagggc
     8281 tggcgatacg tttacggtgg agcttccccg actacttctt ttgattgtag cggactgaca
     8341 caatggacgt atggaaaagc tggaattaac ttaccacgaa ccgcacaaca gcaatatgat
     8401 gtgacccagc atatcccact atcggaagca caagctggcg atttggtttt ctttcattct
     8461 acctataacg ctggctctta tattactcat gttgggatat accttggcaa taaccgtatg
     8521 tttcatgcag gcgacccaat cggttatgcc gacttaacaa gcccctactg gcaacagcat
     8581 ttagtgggag caggacgaat caaacaatga gaaaggaaga tttaatgatg aaatttagaa
     8641 aaaatcagaa taaagaaaaa cagataccaa aggaaaagaa acctcgtgtc tataaggtca
     8701 atcctcataa aaaggttgtg attgccttgt gggtactttt agggcttagt ttcagctttg
     8761 cgatattcaa gcactttaca gctatagata ctcatactat tcacgaaaca actatcatag
     8821 aaaaggaata cgttgatact catcatgtag aaaattttgt agagaacttt gcgaaagtct
     8881 actattcatg ggagcaatcc gataagtcca ttgataatcg aatggaaagt ctaaaaggct
     8941 atctgacaga tgaacttcaa gctctcaatg ttgatacagt acgcaaagat attcctgtat
     9001 cgtcttctgt aagaggattt cagatatgga cggtagagcc aactggcgac aatgagttta
     9061 atgtaaccta cagtgtagac cagctcatta cagagggaga aaatacaaag accgtccact
     9121 ctgcttatat agtgagtgtc tatgtagatg gttctggaaa tatggtactg gttaagaatc
     9181 cgaccattac caacatacct aagaaatcaa gttataaacc aaaagccatt gaaagtgagg
     9241 ggacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg ttcttcaagc
     9301 tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg atattaaaac
     9361 caatcggaaa agagtacatc tttcaagaac tggtaaatcc tattcacaat cgtaaggata
     9421 atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa gcaacgcagg
     9481 tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt gtaaaataac
     9541 aaatattggt acatgattac agatactttg taatcatgta ctctttttga taaaaaattg
     9601 gagattcctt tacaaatatg ctcttatgtg ctattattta agtgactatt taaaaggagt
     9661 taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagcg gaaacaaata
     9721 attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga atacctttat
     9781 catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat ggttatgcat
     9841 aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt gggtttttga
     9901 atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt tgatgcagga
     9961 aaaactacct taacagaaag cttattatat aacagtggag cgattacaga attaggaagc
    10021 gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag aggaattaca
    10081 attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat catagacacg
    10141 ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt agatggggca
    10201 attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt atttcatgca
    10261 cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca aaatggaatt
    10321 gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat tgtaatcaaa
    10381 cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc tgaacaatgg
    10441 gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg taaatcatta
    10501 gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc tctgttccct
    10561 ctttatcatg gaagtgcaaa aagtaatata gggattgat
//
LOCUS       DAVMEI010000058        10597 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVMEI010000058 DAVMEI010000000
VERSION     DAVMEI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10597
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.058"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            157..5067
                     /locus_tag="VYQ24_001148"
     CDS             157..5067
                     /locus_tag="VYQ24_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1968294.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEAASAAKPEEKAGEVVAETPS
                     AEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKTLKPKEIKFNS
                     WEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSNTNSKAKDHAS
                     VGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLFFNWSNSIADQ
                     ERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLGEKMRQFMLYS
                     KEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPADNFFANFNWD
                     KAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGKLRLSLGLFAP
                     DTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADRTSAVGNTFTT
                     SFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAEYDFTDAYNGG
                     NSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAFSTTPDYKFDD
                     ADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNLGQLTISDNHQ
                     EPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKLLTGSSSTTIY
                     LPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFEWGMTVQDTSLPRPLAENIVPG
                     ATVIGSTFPDTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKPRTVVRWVMDH
                     AGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLDKPITAQDWRL
                     NVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQVGFADVPAGA
                     TITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGKEISNVLAVSV
                     PKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIRLTHAGVSVSG
                     FDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQKPKKNYLVGDSL
                     DLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKIGRQTLTLRYQGHEVNFDVLVSP
                     KAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLKDHEISTQDQV
                     NDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLDAKPNHPSGSALAPLLEKNK
                     VLVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSNKEKTVIKGLK
                     VERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFL
                     PEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNTVLPKPTYQPA
                     SDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRL
                     RSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANE
                     ALIAGLASLGLASLALTLRRKREDKD"
     gene            5157..6353
                     /locus_tag="VYQ24_001149"
     CDS             5157..6353
                     /locus_tag="VYQ24_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1968295.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6489..7016
                     /locus_tag="VYQ24_001150"
     CDS             6489..7016
                     /locus_tag="VYQ24_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1968296.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7093..7449
                     /gene="glnR"
                     /locus_tag="VYQ24_001151"
     CDS             7093..7449
                     /gene="glnR"
                     /locus_tag="VYQ24_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1968297.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7486..8832
                     /gene="glnA"
                     /locus_tag="VYQ24_001152"
     CDS             7486..8832
                     /gene="glnA"
                     /locus_tag="VYQ24_001152"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1968298.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(8928..9047)
                     /locus_tag="VYQ24_001153"
     CDS             complement(8928..9047)
                     /locus_tag="VYQ24_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1968299.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            9305..9442
                     /locus_tag="VYQ24_001154"
     CDS             9305..9442
                     /locus_tag="VYQ24_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968300.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            9592..>10597
                     /locus_tag="VYQ24_001155"
     CDS             9592..>10597
                     /locus_tag="VYQ24_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1968301.1"
                     /translation="MPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDT
                     EKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAIS
                     NYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRII
                     EAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLL
                     EKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYGNKDETTSYPIYEIPEAWRYIKF
                     ASLVNFRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDIS
                     P"
BASE COUNT         3464 a         1893 c         2273 g         2967 t
ORIGIN      
        1 aaccttattt tagatttgtg caaacagtta ttgttttttg tctaaaaatg ttttaggata
       61 aaaatgtgat tatcatggtt ctagagtttg ataaaaaact acagaatata aagcgctttc
      121 tttaaaacaa caaaatcaat cttttaggag gagaaaatga agaatccatt ttttgaaaga
      181 cgttgtcgtt acagtattcg taagttatca gtaggagcct gctcgctgat gattggtgct
      241 gttttatttg ctggtccagc cttggctgaa gaaactgcag ttcctgaaaa tagcggagct
      301 aatacagagc ttgtttcagg agagagtgag cattcgacca atgaagctga taagcagaat
      361 gaaggggaac atgctagaga aaacaagcta gaaaaggcag aagcagctag cgcagctaaa
      421 ccagaggaaa aagcaggtga ggtggttgca gaaacaccat ctgcagaagc aaaacctaag
      481 tctgacaagg aaacagaagc aaagcccgaa gcaactaacc aaggggatga gtctaagcca
      541 gcagcagaag ctaataagac tgaaaaagaa gtccagccag atgtccctaa aaatacagaa
      601 aaaacattaa aaccaaagga aatcaaattt aattcttggg aagaattgtt aaaatgggaa
      661 ccaggtgctc gtgaagatga tgctattaac cgcggatctg ttgtcctcgc ttcacgtcgg
      721 acaggtcatt tagtcaatga aaaagctagc aaggaagcaa aagttcaagc cttatcaaac
      781 accaattcta aagcaaaaga ccatgcttct gttggtggag aagagttcaa ggcctatgct
      841 tttgactatt ggcaatatct agattcaatg gtcttctggg aaggtctcgt accaactcct
      901 gacgttattg atgcaggtca ccgtaacggg gttcctgtat acggtacact cttcttcaac
      961 tggtctaata gtattgcaga tcaagaaaga tttgctgaag ctttgaagca agacgcagat
     1021 ggtagcttcc caattgcccg taaattggta gacatggcca agtattatgg ctatgatggc
     1081 tatttcatca accaagaaac aactggagat ttggttaaac ctcttggaga aaagatgcgc
     1141 cagtttatgc tctatagcaa ggaatatgct gctaaggtaa accatccaat caagtattct
     1201 tggtacgatg ccatgaccta taactatgga cgttaccatc aagatggttt gggagaatac
     1261 aactaccaat tcatgcaacc agaaggagat aaggttccgg cagataactt ctttgctaac
     1321 tttaactggg ataaggctaa aaatgattac actattgcaa ctgccaactg gattggtcgt
     1381 aatccttatg atgtatttgc aggtttggaa ttgcaacagg gtggttccta caagacaaag
     1441 gttaagtgga atgacatttt agacgaaaat gggaaattgc gcctttctct tggtttattt
     1501 gccccagata ccattacaag tttaggaaaa actggtgaag attatcataa aaatgaagat
     1561 atcttcttta caggttatca aggagaccct actggccaaa aaccaggtga caaagattgg
     1621 tatggtattg ctaacctagt tgcggaccgt acgtcagcgg taggtaatac ttttactact
     1681 tcttttaata caggtcatgg taaaaaatgg ttcgtagatg gtaaggtttc taaggattct
     1741 gagtggaatt atcgttcagt atcaggtgtt cttccaacat ggcgctggtg gcagacttca
     1801 acaggggaaa aacttcgtgc agaatatgat tttacagatg cctataatgg cggaaattcc
     1861 cttaaattct ctggtgatgt agccggtaag acagatcagg atgtgagact ttattctact
     1921 aagttagaag taactgagaa gaccaaactt cgtgttgccc acaagggagg aaaaggttct
     1981 aaagtttata tggcattctc tacaactcca gactacaaat tcgatgatgc agatgcatgg
     2041 aaagagctaa ccctttctga caactggaca aatgaagaat ttgatctcag ctcactagca
     2101 ggtaaaacca tctatgcagt caaactattt ttcgagcatg aaggtgctgt aaaagattat
     2161 cagttcaacc tcggacaatt aactatctcg gacaatcacc aagagccaca atcgccgaca
     2221 agcttttctg tagtgaaaca atctcttaaa aatgcccaag aagcggaagc agttgtgcaa
     2281 tttaaaggca acaaggatgc agatttctat gaagtttatg aaaaagatgg agacagctgg
     2341 aaattactaa ctggctcatc ttctacaact atttatctac caaaagttag ccgctcagca
     2401 agtgctcagg gtacaactca agaactgaag gttgtagcag tcggtaaaaa tggagttcgt
     2461 tcagaagctg caaccacaac ctttgagtgg gggatgactg tccaagacac aagtcttcca
     2521 agacctctag cagaaaacat cgttccaggg gctactgtta tcggaagtac cttcccagat
     2581 acagaaggtg gagaaggtat tgaaggtatg ttgaacggta ccattactag cttgtcagat
     2641 aaatggtctt cagctcagtt gagtggtagt gtggatattc gtttgaccaa gccacgtacc
     2701 gttgttagat gggtcatgga tcatgcaggg gctggtggtg agtctgttaa cgatggcttg
     2761 atgaacacca aagactttga cctttattat aaagatgcag atggtgagtg gaagctagct
     2821 aaggaagtcc gtggcaacaa agcacacgtg acagatatca ctcttgataa accaatcact
     2881 gctcaagact ggcgcttgaa tgttgtcact tctgacaatg gaactccatg gaaggctatt
     2941 cgtatctata actggaaaat gtatgaaaag cttgatactg agagtgtcaa tattccgatg
     3001 gccaaggctg cagcccgttc tctaggcaat aacaaggtac aagttggctt tgcagatgta
     3061 ccggctggag caactattac cgtttatgat aatccaaatt ctcaaactcc gcttgcaacc
     3121 ttgaagagcg aagttggagg agacctagca agtgcaccat tggatttgac aaatcaatct
     3181 ggtcttcttt attatcgtac ccagttgcca ggcaaggaaa ttagtaatgt cctagcagtt
     3241 tccgttccaa aagatgacag aagaatcaag tcagtcagcc tagaaacagg acctaagaaa
     3301 acaagctacg ccgaagggga ggatttggac cttagaggtg gtgttcttcg agttcagtat
     3361 gaaggaggaa ctgaggacga actcattcgc ctaactcacg caggtgtatc agtatcaggt
     3421 tttgatacgc atcataaggg agaacagaat cttactctcc aatatttggg acaaccagta
     3481 aatgctaatt tgtcagtgac tgtcactggc caagacgaag caagtccgaa aactattttg
     3541 ggaattgaag taagtcagaa accgaaaaaa aattacctag ttggtgatag cttagacttg
     3601 tctgaaggac gctttgcagt ggcttatagc aatgacacca tggaagaaca ttcctttact
     3661 gatgagggag ttgaaatttc tggttacgat gctcaaaaga ttggtcgtca aaccttgacg
     3721 cttcgttacc aaggtcatga agtcaacttt gatgttttgg tatctccaaa agcagcattg
     3781 aacgatgagt acctcaaaca aaaattagca gaagttgaag ctgctaagaa caaggtggtc
     3841 tataactttg cttcaccaga agtaaaagaa gctttcttga aagcaattga agcggccgaa
     3901 caagtgttga aagaccatga aattagcacc caagatcaag tcaatgaccg acttaataaa
     3961 ttgacagaag ctcataaagc tctgaatggt caagagaaat ttaaggaaga aaagacagag
     4021 cttgatcgtt taacaggtga ggttcaagaa ctcttggatg ccaaaccaaa ccatccttca
     4081 ggttctgccc tagctccgct tcttgagaaa aacaaggtct tggttgaaaa agtagatttg
     4141 agtccagaag agcttgcaac agcgaaacag agtctaaaag atctggttgc tttattgaaa
     4201 gaagacaagc cagcagtctt ttctgatagt aaaacaggtg ttgaagtaca cttctcaaat
     4261 aaagagaaga ctgtcatcaa gggtttgaaa gtagagcgtg ttcaagcaag tgctgaagag
     4321 aagaaatact ttgctggaga agatgctcat gtctttgaaa tagaaggttt ggatgaaaaa
     4381 ggtcaagatg ttgatctctc ttatgcttct attgtgaaaa tcccaattga aaaagataag
     4441 aaagttaaga aagtattttt cttacctgaa ggcaaagagg cagtagaatt ggcttttgaa
     4501 caaacggata gtcatgttat ctttacagca cctcacttta ctcattatgc ctttgtttat
     4561 gaatctgctg aaaaaccaca acctgctaaa ccagcaccac aaaacacagt ccttccaaaa
     4621 cctacttatc aaccggcttc tgatcaacaa aaggctccta aattggaagt tcaagaggaa
     4681 aaggttgcct ttcatcgtca agagcatgaa aatgctgaga tgctagttgg ggaacaacga
     4741 gtcatcatac agggacgaga tggactgtta agacatgtct ttgaagttga tgaaaacggt
     4801 cagcgtcgtc ttcgttcaac agaagtcatc caagaagcga ttccagaaat tgttgaaatt
     4861 ggaacaaaag taaaaacagt accagcagta gtagctacac aggaaaaacc agctcaaaat
     4921 acagcagtta aatcagaaga agcaagcaaa caattgccaa atacaggaac agctgatgct
     4981 aatgaagccc taatagcagg cttagccagc cttggtcttg ctagtttagc cttgaccttg
     5041 agacggaaaa gagaagataa agattaaata tcgaaaaatc ttgtgaaatc tttccttata
     5101 tttctaaagt gtgatataat agtttcgaat aaaataaata aaggagtttt tgtaacatgg
     5161 caaaacttac tgttaaagac gttgacttga aaggtaaaaa agtcctcgtt cgtgttgact
     5221 tcaacgtacc attgaaagat ggcgtaatca ctaacgataa ccgtatcaca gcagctcttc
     5281 caactattaa gtacatcatc gaacaaggtg gacgtgcaat tcttttctct caccttggac
     5341 gtgtgaaaga agaagctgat aaagctggta aatcacttgc tcctgtagca gctgacttgg
     5401 cagcaaaact tggtcaagat gttgttttcc caggtgtcac tcgtggtgct gaattggaag
     5461 cggcaatcaa cgctcttgaa gatggacaag ttctcttggt tgaaaacact cgttacgaag
     5521 atgttgacgg caagaaagaa tctaaaaacg atcctgaact tggtaaatac tgggcatcac
     5581 ttggagatgg tatcttcgta aacgatgcat tcggtacagc tcaccgtgca cacgcatcta
     5641 acgttggtat ctcagcaaac gttgaaaaag cagttgctgg tttccttctt gaaaacgaaa
     5701 ttgcctacat ccaagaagca gttgaaactc cagaacgtcc attcgtggct atccttggtg
     5761 gttcaaaagt ttcagacaag atcggtgtta tcgaaaactt gcttgaaaaa gctgataaag
     5821 tccttatcgg tggtgggatg acttacacat tctacaaagc acaaggtatc gaaatcggta
     5881 actcacttgt agaagaagac aaattggatg ttgcgaaagc tcttcttgaa aaagcaaatg
     5941 gtaaattgat cttgccagtt gactcaaaag aagctaacgc atttgctggt tacactgaag
     6001 tgcgtgacac tgaaggtgaa gcagtttctg aaggcttcct tggtcttgac atcggtccaa
     6061 aatctatcgc caaatttgac gaagctttga ctggtgccaa aacagttgta tggaacggac
     6121 ctatgggtgt atttgaaaac ccagatttcc aagctggtac aatcggtgtg atggacgcta
     6181 tcgtgaaaca accaggagtt aaatcaatca tcggtggtgg tgactcagct gccgcagcga
     6241 ttaaccttgg ccgtgcagac aagttctcat ggattagtac gggtggtgga gcatcaatgg
     6301 aacttcttga aggtaaggtt cttccaggac ttgcagcctt gacagaaaaa taagatttta
     6361 taaacaaatc aaagaagaga gggatgaaag ttcctctttt cttttgctta aaataaaaac
     6421 gcttcctctc aactattact cataaaaatc accgatttat gataaaatgg aaatagaaag
     6481 ttgagattat gagttatttt aaaaaatata aattcgataa atcccagttc aaacttggta
     6541 tgcgaacctt taaaacaggt attgctgttt ttctagttct cttgattttt ggcttttttg
     6601 gctggaaagg tcttcaaatt ggtgctttga cagccgtttt tagcctgagg gagagttttg
     6661 atgagagtgt tcattttggg acttcgcgta ttctaggaaa tagtatcggt ggactctatg
     6721 ccttggtctt cttcttatta aatacctttt tccacgaagc cttttgggtg accttggtag
     6781 ttgttccaat ctgcaccatg ttaaccatta tgacaaatgt agccatgaat aacaaagcag
     6841 gggttattgg tggtgtagca gctatgttaa tcattaccct atcaattcca agtggtgaga
     6901 caatttcgta cgtgtttgtg cgtgtatcag aaacgtttat gggagttttt gttgcaatta
     6961 tcgtaaatta cgatattgat cgtattcgtc tctttttaga gaaaaaagaa aaataatgtt
     7021 acattttata acattatcaa ttgacgtttg tcttttttta gactataata gacagaaaga
     7081 aggaaattgt aaatgaagga aaaagaattt cgccgaaata tggctgtttt tcctatcggc
     7141 agtgttatga agttgaccga tctatcggcg cgtcagattc gttattatga agatcaagag
     7201 ttgatcaagc ccgatcgaaa cgaaggaaat cgtcgcatgt attccttgaa tgacatggat
     7261 cgtctgcttg aaatcaaaga ttatatctct gaaggttata atatcgctgc cattaagaaa
     7321 aaatatgctg aacgtgaagc gaaatccaag aaagcggtta gtcagactga agtacgtcgt
     7381 gcacttcaca atgaactcct ccaacagggg cgttttgctt cagtacagtc accttttggt
     7441 cgcggttagg caaccgcaag tagtcataga ttaaggagaa aacttatgcc aatcacagct
     7501 gcagatattc gtcgtgaagt caaggaaaaa aatgttacct ttattcgtct tatgttctca
     7561 gatattttgg gaaccatgaa aaacgtcgaa attcctgcta cagatgaaca gttagataag
     7621 gtcttgtcga acaaggttat gtttgatgga tcttctattg aaggttttgt acgtatcaat
     7681 gagtcggata tgtacttgta tccggacttg gatacatgga cagtcttccc ttggggagat
     7741 gaaaatggaa gtgttgcagg tctgatctgt gatgtctata caacagaagg tgaaccattt
     7801 gcgggtgacc ctcgtggtaa tttgaaacga gctcttcgtc acatggaaga agttggattc
     7861 aaatccttca accttggtcc agagccagaa ttcttcctat ttaagttgga tgaaaatggg
     7921 gacccaacac ttgaagtgaa tgacaagggt ggctactttg atttggcacc tactgacctt
     7981 gcggacaaca cacgtcgtga gattgtgaat gtcttgacca aaatgggatt tgaagtagaa
     8041 gcgagtcacc acgaggttgc ggttggacag catgagattg actttaagta cgatgaagtt
     8101 ctccgtgctt gtgataagat tcaaatcttt aagcttgttg ttaaaaccat tgctcgcaaa
     8161 cacggacttt acgcaacatt tatggcgaag ccaaaatttg gtattgctgg atcaggtatg
     8221 cactgtaata tgtccttgtt tgatgcagaa ggaaataacg ccttctttga tccaaatgat
     8281 ccaaaaggaa tgcagttgtc agaaacagct taccatttcc taggcggttt gatcaagcat
     8341 gcttacaact atactgccat catgaaccca acagttaact catacaaacg tttggttcca
     8401 ggttatgaag cgcctgttta cattgcttgg gctggtcgta accgttcgcc acttgtgcgc
     8461 gtacctgctt cacgtggtat gggaactcgt cttgagttgc gttcagtgga tccaatggcg
     8521 aacccttacg ttgctatggc tgttcttttg gaagttggtt tgtatggtat tgaaaataaa
     8581 atcgaagcac cagctcctat cgaagaaaat atctacatca tgacagcaga agagcgcaag
     8641 gaagctggta ttacagacct tccatcaact cttcacaacg ctttgaaagc tttgacagaa
     8701 gatgaagtgg ttaaagctgc tctcggagat cacatctata ctagcttcct tgaagccaaa
     8761 cgaatcgaat gggcaagtta tgcaaccttc gtttcacaat gggaaattga taattattta
     8821 gacctttact aatactaata tagaagaaag attgcctgtg ggtaaacagt cacacagcag
     8881 tctaagccat caaataccta taacccttaa aatagcgatt tcggtcgcta tttatttttc
     8941 gaaaaatcct cttgactctt ttgcgaatta gatttatcat cagacgtgta gaagaaaatc
     9001 aaggagtcac tatggaaaaa ttcaatttca aaaacaatat cgggcaagaa aacaagttac
     9061 ttcaaatcga aatctacaag tttactaact tttgcaagct tcaaaattac acatctgtaa
     9121 atattttttc gaaagatata tttgaagcca tagtaaattg agactagaat agtacactac
     9181 atctgctaaa aatttctaga aattaattta actttcctaa tcgatttgtt tacatcttat
     9241 ttcaatctac tatattatct caataaatga tgatggcgac tgttttgttt gtgcttgtcg
     9301 atttttgaat cctgatcgag cggaagaata tttctgccga ggttgtcaag gggaaaatcc
     9361 tgaagatatt gaattttatg atgaacaatt acaggcagaa aaggtggagg ctttgaatat
     9421 tcgattggaa gtaaaaaatt gaattatcta caatctcatc aacaataaac ctatttcaac
     9481 cccattatct ataggcgtat ttttacgcct ttttatatac ttgaagcaca taagaaacat
     9541 tatgggaagt ttgctgatgg aagcactcaa gaaattgatg ttccttatga tattcctgat
     9601 acttgggagt gggtgaggtt ttctacattg gttgaaattg tcagaggtgg ctctccacga
     9661 ccaatcaaag attatcttac ttctgaagta gatggaataa attggataaa aataggtgat
     9721 actgaaaagg gtgaaaagta tataaataat gttaaagaaa aaatcaaaaa atcagggctt
     9781 aacaaaacta gatttgtaaa aaaaggtaca tttttgttaa ctaattctat gagttttggt
     9841 agaccttata ttttgaatgt tgatggtgca atacacgatg gatggttggc tatttcgaac
     9901 tatgaaaact cattaaataa agattaccta ttctatattc tttcatcaaa tgtagtttat
     9961 tctcaatttc tatctctaat tagtggagct gttgtgaaaa acttgaatag tgataaagtt
    10021 gcttctattc ttatccctct ccccccacta tccgaacaac aacgcataat agaagcaatc
    10081 gaatcagctt tagaaaaagt agatgaatat gctgaaagtt ataatagact agaacagcta
    10141 gataaagaat ttccagataa actaaaaaaa tctattcttc aatatgctat gcaaggaaaa
    10201 ttagttgaac aagacccaaa tgatgaatca gtcgaagttt tacttgaaaa aatacgagca
    10261 gaaaaacaaa aactctttga agaaggcaag attaaaaaga aagatttgga catttctatt
    10321 gtttcccaag gagatgataa ctcttattat gggaacaaag acgaaacaac ctcttatcct
    10381 atatatgaaa tcccagaagc atggagatat attaaatttg ctagcctagt taacttcaga
    10441 ataggaaaaa ctcctccacg cagtgaagct actttttggg gaactgaaat accttgggta
    10501 tctatatcag atatgcctat atctggttat gtaactaata cgagagaatc tatttctaaa
    10561 ttagcattaa aatcaaagaa aatagacatt tcaccta
//
LOCUS       DAVMEI010000059        10533 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVMEI010000059 DAVMEI010000000
VERSION     DAVMEI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.059"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(36..1460)
                     /locus_tag="VYQ24_001156"
     CDS             complement(36..1460)
                     /locus_tag="VYQ24_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1968302.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2005..4284)
                     /gene="pulA"
                     /locus_tag="VYQ24_001157"
     CDS             complement(2005..4284)
                     /gene="pulA"
                     /locus_tag="VYQ24_001157"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1968303.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4403..6361)
                     /gene="ligA"
                     /locus_tag="VYQ24_001158"
     CDS             complement(4403..6361)
                     /gene="ligA"
                     /locus_tag="VYQ24_001158"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1968304.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6454..7632)
                     /locus_tag="VYQ24_001159"
     CDS             complement(6454..7632)
                     /locus_tag="VYQ24_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1968305.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7712..8575)
                     /locus_tag="VYQ24_001160"
     CDS             complement(7712..8575)
                     /locus_tag="VYQ24_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1968306.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQKAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8578..10479)
                     /locus_tag="VYQ24_001161"
     CDS             complement(8578..10479)
                     /locus_tag="VYQ24_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1968307.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKYSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3160 a         2334 c         1890 g         3149 t
ORIGIN      
        1 ataaaaccag gaaaacaatt tcctggtttt acacgttatt tcacatcaaa aacaatggat
       61 ttgacatttg tcatcgcttc aatgctatat ttaattcctt gcactccagc tccagaacct
      121 ttgacaccaa ggaatgggaa attatctgga ccacgctggg ttttattatt aatgtggact
      181 gtacctactt caagtttttc agcaatttca aaggcttttt tgaaatcatt tgtaaagact
      241 gatgattgaa ggccgaattc agattcgttg gcaaaggcaa tagcttcctc tacactagcc
      301 acacgaatga ttggtaaaac aggaccaaat ggctcttccc atgccacttt catatctttt
      361 gtaacttggt caaaaagcac tggccagaga agattgccct cacgtttgat tggtgtaaga
      421 gcctgagctt ctttttcttg tgcatcctca atcaagcccc aaatgaagtc ggctgaagca
      481 ttgtcaataa caggtgtaat atcagcattg tcaaatggat caccaactgt taatttagaa
      541 acttcttcct gaagcaaagt agctaattta tctgctacac tttcgagaac aatgacacgt
      601 ttaatggccg tgcaacgttg tcctgagtag ctaaaggctc ccgcaacaat ttgcttggca
      661 gcatgttcca aatctgcatc ttctagtaca agagctgcat ctttcccacc aagttccaac
      721 atgataggac gcataccagc taaacgacca atacgttctc caataggagt tgaacctgta
      781 aagttgatga agttgacttc tttgtgctca atgatataat ccccaatttc tgaaccacga
      841 cctgtaatgg tattgaaaac acctgccgga atccctgctt cttcaaatgc tttagccaac
      901 aagagtccag aaatggaacc ttgtgttggt ggcttaaaca tgaccacatt ccctgcaatc
      961 aaggcaggtg caattttaga agcagataaa ttaactggat aattaaaggg agcaatcgct
     1021 agcacgatac caactggttc acgacggaca acagccagtt tgtttttact tgctgcctca
     1081 aaaccaccac cttccattgc ttgtccagtg atacggagac cttcctcagc agcataacga
     1141 atcaagtctg ctgtacgcac tacttctcca attgctgctt taatcccttt tgctacttct
     1201 ttggcaagga tagtaccaat ttcttcctta tcgcgttcta aaatagctgc tgttttatgt
     1261 aaataagccg cacgttcaac tgctgataaa gctcgccatg ctggcagggc tgcacgcgca
     1321 gcttgcatag cctcatcagc ttcagtctga gtcatggctg gaactgtacc caattcttct
     1381 tgattgattg gtgaataaat cgtaatttct tgttcagatg atttccattt tccatttact
     1441 aaattctgat atcttgtcaa attcttgtcc tcctgaaaat gattaagata tattttacca
     1501 aattttcaga aaatcacaac cctttttgca aaatttttga gattattttc acaaacttga
     1561 tttttcaaag tatactcaat aaaaataaaa aaaatccact acgtcaaggc gaggctaatg
     1621 tggtttgaag aaattttcga agagcgtgaa tgagtatcat ctatagtaaa ataaaaaaac
     1681 tgaacaattt ggttggggac agccaaacca atttctcaca atgtttcaga aacaagggtg
     1741 tgctactcca atttcagcct actataactg tcatagattg ctgaaacaaa gtctaggtaa
     1801 aagtcttcat aataaaaaga cctcctatca agtgttcaaa aactttgata ggaggtcttg
     1861 ttttgtgaaa atatttatca aattttctat acaagtgagc tgttagccag gttctttcta
     1921 ttctttcaat ttcaatgaat ggatttttta ctaatactca taactgggaa tttgtctgtg
     1981 taaaaatagc gagatagatg gtatttataa aacactcaag acagctagac taatatcatt
     2041 taaaacatta tcttcttttg agcgactgtt ggttaccaac atagctaaat ttcctgcatt
     2101 ttcaaattga tagggttctg atttagcatt cacaaccacc aagaggtgtt ctttgccgtg
     2161 aacttcatag ataaggtagc cgctatgttc aatcgcagaa tgcacaaaga catgatggta
     2221 aatttcatca tagctagagt aagaaaaggc accagttttt gtcttcaatc ggatgacttg
     2281 acggataaac tcaatactgt cttgacgctc attaatcaag ttccagttca cttggttcac
     2341 actgtcagga gcattatagc tattcatcgc acgctctcta tcatcatggg tcaactcacc
     2401 attttcacca gtcgcaacca gtttggtacg accaaattct tgaccgattt ccataaaggc
     2461 catcccctgc atgagcagat tcatggctgt ggcagtttcg accttgcgca tgatttgctc
     2521 tgaactttgg tctggatgaa gggttgccaa taaatcgtga agattgtaat tgtcatgggc
     2581 ttctacatag ttaagcacct gatttggatg tgtatagctt cctaattcac gacttcctag
     2641 gattgcttta gctagaattg gctctgtcgc agcaccactg acaaaacctg acttgatagc
     2701 accataaact tctccccctt tgacagcatc gcgctgattg tcattaaaga aaccaatatt
     2761 tggcatctgg taggcattgt ccttcttggc cttatcataa ggggcaagac ctgttcccat
     2821 atcccatcct tctccataga ggataatgtt ggagtcgatt tcatccaagc tttgacgaat
     2881 catctgcatg gtcttgacat catgaatccc catcaagtca aaacggaagc cgtcaatatt
     2941 atattcctgc acccagtata gaagagaatc aatcatatac ttgcgaaaca tttcgtgttc
     3001 actggctgtt tcatttccaa cacccgttcc attctggaag gtaccatctg gattcatacg
     3061 ataatagtaa tcagggactg ttgtttggaa tggtgcatca acaactgaga aggtatggtt
     3121 atagactaca tccataatga ctccaatacc cgcatcgtga taagcttgaa ccatcacctt
     3181 caaatcacga atgacctgag ctggatcatc tggattagtt gaaaaactag tttctggcgc
     3241 gttatagttt tgtggatcat aaccccagtt gtaggttaca tttccatcct catcgtattc
     3301 tttatgacgg tctgcaattg gttgcaattg aacataattg tagcccagct tcttgatgta
     3361 atcaaaagca gttgactggc cgtattggtt aactgttcca gcctgagcag cacccaagaa
     3421 agttcctcga agatgttcat ctacacccga tgtaggtgat ttagtcaaat cacgaatatg
     3481 catttcacag ataactgcct tacatggatt ttccaagcgc caagtagcct ccgaaccgtg
     3541 cttaacctcg aagttttcaa cttgcttttc tacatggctc agaatagctg aacgtttgcc
     3601 atcagggctg gtcgcgattg tataaggatc acgtgtcagt gtttggtgat gagggaattg
     3661 gacttgatac tgataagcct tacctaccaa atcttcctca acatccaaac tccagacacc
     3721 gattgtattg tccttatgat tataagagta gctattgcct cttttcatct caaaagtctt
     3781 ccaaacgggt gcatcattag cagctgattc ataaacgaca acttgcactt ctgtcgctgt
     3841 aggtgaccag agggaaaaat gagcctgatt gtcctctaca cggcaaccca attctccttg
     3901 gtaaccccaa tgatggtcaa aactagcact gttaatggcc ttatcaaagg caaaaggatt
     3961 ttgattttta tagaaaggac tggcaatagc aggattttca gagtaataaa tcctatcatc
     4021 gccttccaaa atccagacct ctgttaatag gggatagtga ttaaaacgga tagaatattc
     4081 tttactagtt tgacctgtat gaaccacaaa attcaagctt tctataacat gtgaacttgg
     4141 gtgttcaaag ctaaataaag ctccaaaata atcttctttg taggttagca aatcaattcg
     4201 ttgatcctga ctttttacaa aggagcaagt gtcatattct ccattcttac gatggtaatg
     4261 aatgcgcata gggtagttat acatttttta ttttttattt ttccttttta ctttgtttct
     4321 atttcactaa taaatttttg tcaatctcgt ctcaattaac agacatagtc atattctcta
     4381 aactctgttt ttaaacgatc cattacaaac tttctagcca tgcctcatct ctgacctgga
     4441 taccaagttc ttgtgctttt tgcagtttac ttccagcgtc tgcacctacc acgacgaggt
     4501 cggtcttttt agaaatacta cctgtcactt tggcacccag actttcgagt ttacttttag
     4561 cttctgagcg cttgagtcgt tccaattttc ctgtcaatac cacggtcaaa cctgacaagg
     4621 ccgcatccgc tactaccgtc tgtcctttat agtccagatt gaccccagtt tctttcaatt
     4681 ctctgagcag aatttcagag ccttctgtcg caaaataagt ctgaagactt ttggcaatca
     4741 cgccacctag actttcaata ctagccactt cctctgaatc tgcctgagac agattttcaa
     4801 ttgaatggaa atattgaagt aaaagctgac taaccttgct tccgacatga cgaattccca
     4861 aaccaaataa gagcttctcg gcagaatttt cctttgatgc ttggatagcc tgatacagtt
     4921 tagcagcgga cttttcctta accccctcta aaaggaggaa atcctcttct tgcaaacgat
     4981 aaatatccgc cacatccttg actaaattag cagcaaaaag cttctcaaca atagatggac
     5041 caaggcctgt aatattcata gcatcacgag aagcaaagtg aatcaagcct tccatgattt
     5101 gagcagggca acgcggattg atacaacgta gggccacttc atcttcaaag tgcaacaagt
     5161 cagagttaca acttggacag tttgtaggga tatctagttt ttcttcagaa acccgtttgg
     5221 actctaccac acgtaaaacg gcagggatga tgtcaccagc cttatataca atgaccgtat
     5281 cgtcttttcg gatatctttt tcagcaatat aatctacatt gtgcagggtc gcacggctaa
     5341 cagtcgtacc ggcaagttgt actggtgtta gattagcagt tggagttaca acaccggtac
     5401 ggccaactgt ccagtcaact gataagagtt gagcttcttt ttcttcggca gggaacttgt
     5461 aggctactgc ccactttgga gccttaactg taaaaccaag ttcttcttga cttgctaggt
     5521 cgttgacctt gattaccact ccatcaatat cgtaaggcag attttcccgt tcctgtccta
     5581 cttcttggat aaaattccag atttcatcta tgttttcagc caagattcgc ttaggattga
     5641 ccacaaaacc tagttgttct aggtacttca aacccttttc ttggctatca cgagttgaag
     5701 ggctggcttc ttgatagaga aacgttgcaa gattacgctt ggcaactact gctgtatcca
     5761 actgacgcag agttcctgct gccgcattac gaggattagc aaattcaggc tctccatttt
     5821 cttggcgcac ttggttaact tggtcaaagg aagcgcgtgg catgtaacat tccccacgaa
     5881 ctgtgatatc tagttcttct ggcaaagtca aagggatgtc cttaacacgc ttgaggtttt
     5941 ctgtgatatt ttcaccaatt gaaccatctc cacgtgttac cccagcaacc aaaatcccct
     6001 tttcataagt cagcgagata gataagccat cgattttcag ctcacaaata taggtcggat
     6061 gagccacttc cttacgaaca cgcgcatcaa aagcatctag ctcctcacgt gaaaaagcat
     6121 cctgcaaact ataaagagga tactgatgac tgtatttttc aaaaccatct aaaaccttgc
     6181 caccaacacg atgagtcgga ctgtctgcta gcacttgctc tggataagca gtttctaact
     6241 cgaccaactc acggtaaagg cggtcatact cactgtctgc aaccgaggga ttatcgctgg
     6301 tatagtactc agtcgcatag cgattgagca aagcgactaa ctcattcatt cttttattca
     6361 taagaccatt ttaccataaa acaagccctc ctcacaaacg agaagggcgg aaaaaacact
     6421 tagtttgaaa ttatttttga aactcaagca accttatatc aatttttcaa aatgagttcg
     6481 aacataaata aacgatatac aagacaagat gataacacca cttccaatta tcaggaaaga
     6541 agagagatgt acacttggca agactgtcat aaatcctttt gcaataggca taaatagaat
     6601 agctaaggta aaaattgtac tcagtactct tccaagaaat tcgctctcaa ccttggtttg
     6661 tacttgagta aaaaagtgaa tattaaaaat cgtcataaac aattcacaaa ctaaatttcc
     6721 agaaaaggaa agaaaagttg gaagtggtaa tcccatcata aaaactccga cacctgtcaa
     6781 agccagtaaa atcaaaagat tataaatatt agctttaatt ttactagcta gaagagcccc
     6841 aatgatggaa ccaatagccc ccatagttaa aatacttgca taggctcctt ctgacccgta
     6901 aagctgattc gaaaagggaa gtagaaattc aaaagctgca aaaaagaaat taacgctgga
     6961 agctaccagc aaaaggaaga aaatttcttg ctgatgccag atatagtgta acccatcctt
     7021 gatatctaca aaaatatctc tcccagtaaa agcctttttc tcttgaactt ttgcttcctc
     7081 ttttggaagg aaagccacta gaacaaaagc aatgaaaaaa gtcagcgagt ctagcagtag
     7141 cgtcatattg agacttgcaa actgtaaaac aaggaaggaa agaacaggag agctaacacc
     7201 tacaacctgc aaaaccagct ctaagcgaga attatagatc acaatctcat ctttctccac
     7261 cacttcagtt atgatagctt tattggctgt gcgagaaaag gcaaaagcaa tagcctgcac
     7321 aatgttagca acaatcaaag cgccaatcat ccagctatca ttccttatga aagaaatagc
     7381 cagacaaaga atcccacaaa caagatctgc cgtcattaaa atcttacgac gagaaaaacg
     7441 gtctgaaata actccgccaa agggattgac gagaatagat gtgacgagct cagaaatctg
     7501 atacattcct aaaactgtct gtcctatagt ccccatagaa gccaaccaga cactatttcc
     7561 ataatcatag agcatatttc ccattttatt gatagcccca cggctaatca actgcactgc
     7621 atagcgattc atattaaagc tcctctcaaa ttttgaaact attgtatcaa aaccgaaagg
     7681 agctttttat tttttccctt atttgggaaa attaactttt gacaaatttt tcgtagtgtt
     7741 cctgataata ggctacttgc tctggaagac ctaacacatc aaaaatatgc atggcctttt
     7801 gcatctgctt acagccttct ttacactgtc ctttttgata taaggcaaaa ccttttaaat
     7861 aatggaaaac attacgctca taaagcttaa tacctttgtc aataatcttc tctgtataag
     7921 cctcaaaata gttggcatta taaaaagaag aatgctctaa acaatgctgg taacaattga
     7981 gggccaaaat caaaactaat ctcttatggc gactaatctc ttggtaaaat tcctccctct
     8041 ccataacttc tctaccaatc cgagtgacat agtctacatc gtagaaacta tagaggttac
     8101 cgaaaagaat caactcatac atggtccatt cttctgtttt gaagagataa tctgctacct
     8161 tatccaaatc atcctgcttc atatcataag tcgcatctct ttgacaaatc agaccttgta
     8221 gcaaaatcca gttcagctca aaataaaggg aagtcgtcga actcttagac ttttcaagtt
     8281 gttctctttg aagcttttga aaacctgcaa tatcgtttga atagtaaagt gggataatct
     8341 gtgccatcat agacacatgt tcatgattat gaaaattcct tgccttatcc atgaaatttt
     8401 cgattgttac atgaatgtta tccaaaatct caaagaaacg ggagactgcc aggtcagact
     8461 ccccaagctc aaagcgagat aactgagagg tagagcagga ttcgcctgct gcttccttta
     8521 aagaataatt tccacttgtt cgaaattcac gaaatacttt tccaagatgt tccatcttta
     8581 cacctgatct gataattctt cccactcaag catagcttct tcctgacgat ggctgatttt
     8641 gtccagctca gcctgtaatt ccatgagttt gtcggcatcg tttgtttcca acatttgttc
     8701 agaaatggct tggctttgac tttctagctc ttcaatttca gcttctagac tttcgatttg
     8761 tcgcatgagt ttgcgaactt ctttttgact ttctttctgg gcctgatagt cattgactgg
     8821 acttgcttcc tttgcttgat tgctagttga agcttcctca gtctgactca tttctgctgt
     8881 tgctttcttc tcaacatagt agtcgtaatc tccaaggtag agagttgaac cattctcaga
     8941 caattccaaa acatgagttg ccacacgatt gataaagtaa cgatcatgac tgacaaaaag
     9001 caaggtacca tcaaagtcaa tcaaggcatt ttctagcact tccttactat caatatccaa
     9061 gtggttggtc ggctcatcca gaatcaaaaa gttattgttt tccatagaca atttagctaa
     9121 aagcaaacga gctttttcgc caccagatag catgccgact gattttttaa catcatctcc
     9181 tgagaaaagg aaggccccaa gacggttgcg gatttcaact tctggtgtca gtttgaaatc
     9241 attccagagt tcatccagca ccgtattact tggtgtcagc ttgctttggg tttggtcata
     9301 gtaaccaacc tcaacattag cgccaaagcg cttttcacct ttgataaaag gaatctggtc
     9361 cacaatagac ttgataaagg ttgacttgcc gataccattt ggaccaacga tagcgacagc
     9421 attcatctta cgaagatcta ggttgatagg ttgtgacaaa acttccccgt cataaccaat
     9481 agcagtattt tcaacagtca aaacaacatt gcccgacgtt ttttcagact ggaaggtcat
     9541 gttggctgct ttcttgccag cttcaggctt gtccaaacgc tccatttttt ctagttgttt
     9601 acggcgagat tgagcacgtt tggtcgttga agcacgaact agattgcgat tgacaaagtc
     9661 ttccagagca gcgatttcct tctgttgctt ttcatagttt tttgcctcag tagctagctt
     9721 ttgctccttc aactcaacaa aacgagagta attccccaca tagcgatcca aggaatactt
     9781 ggtcaaatct agcgtaattg tcgcaacctt gtccaagaaa taacggtcgt ggctgacgat
     9841 aatgagggca ccgctatagt ttaccaagta attctctagc caggagatgg tttcaatatc
     9901 caagtggtta gttggctcgt ccaagaccaa gagattgggc ttttcaagga gcattttagc
     9961 tagagccaaa cgagtatttt gaccaccaga aagctcagca attttcatct gccacataga
    10021 ctcgtcaaac ttgaatccat tcaaaatcgc tcgaatatca gcttcatagg taaagccacc
    10081 tgcttggcga aaattttcag ataagcggtc ataatctgac atcagtttat ccaaatcctc
    10141 accagacttt tcacccatct ccagctccat ctgacgcagt tgtttatctg tccgacgtaa
    10201 gtcatcaaag acatgaagca tctcatcata gatggtattt tcagactcaa aacggctatc
    10261 ttgagctagg taagacagag aaatatcttt tttcttattg atttctccac tagttggctc
    10321 ctcttctcca actaaaatct tcaaaagagt agacttacct gcaccatttt tcccaacaag
    10381 ggcaatccga tctcgttcat caacctgcag gttgatatta tcgaaaagaa cctctcctgc
    10441 aaaagaacgt tcaattttat tagcttgtaa aataatcata caagtagtat agcatgtttc
    10501 cctaaggcat tcaagataat cgtaagtctt tta
//
LOCUS       DAVMEI010000060        10439 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVMEI010000060 DAVMEI010000000
VERSION     DAVMEI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.060"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(52..1374)
                     /locus_tag="VYQ24_001162"
     CDS             complement(52..1374)
                     /locus_tag="VYQ24_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1968308.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTIRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSYFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1465..1553)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1717..4053)
                     /locus_tag="VYQ24_001163"
     CDS             complement(1717..4053)
                     /locus_tag="VYQ24_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1968309.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFSDIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4157..4705)
                     /locus_tag="VYQ24_001164"
     CDS             complement(4157..4705)
                     /locus_tag="VYQ24_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1968310.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4702..5004)
                     /gene="zapA"
                     /locus_tag="VYQ24_001165"
     CDS             complement(4702..5004)
                     /gene="zapA"
                     /locus_tag="VYQ24_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1968311.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5091..5972
                     /locus_tag="VYQ24_001166"
     CDS             5091..5972
                     /locus_tag="VYQ24_001166"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1968312.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQETNRFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5976..6590
                     /gene="lepB"
                     /locus_tag="VYQ24_001167"
     CDS             5976..6590
                     /gene="lepB"
                     /locus_tag="VYQ24_001167"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1968313.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6718..9084
                     /locus_tag="VYQ24_001168"
     CDS             6718..9084
                     /locus_tag="VYQ24_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1968314.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGITGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDAEFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(9132..10415)
                     /locus_tag="VYQ24_001169"
     CDS             complement(9132..10415)
                     /locus_tag="VYQ24_001169"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1968315.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLTELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         2954 a         2393 c         2136 g         2956 t
ORIGIN      
        1 ctgataaaaa ttatttatag gcaaaaaaat cacacgagct gtgtgattcc attatttgtc
       61 aaaatacttt ttagtttcag caataacgac tggcgacaag accaagaggg caatcaagtt
      121 tggcagagcc atcaaggcgt taacgatatc tgcgataatc cagaccatat ccaactcgat
      181 aaatcctcct aacaagacca tgagcacaaa aaccacacgg tagagccaga taaagcgagc
      241 cccaaagagg aactcaaaac agcgttctcc gtaatagttc caacctagaa tcgttgtaaa
      301 ggcaaaaagt acaaggaaga tggtcaagag ggcaggccca aagtatgaaa agactgttga
      361 gaaagctgac tgagtcaagg caaccccatt caagtcacca ctccaaactc cagttaccaa
      421 gatggtcaaa ccagttagag tacaaatgat gagggtatca ataaaggttc ctgtcatgga
      481 aatcaaacct tgctctactg gttcatttgt cttggcagct gcagctgcaa taggagcaga
      541 acccagacca gattcgtttg agaacacacc acgcgccaca ccattttgaa tagccatccg
      601 aacgctagca ccagcaaatc cacctaccgc agcaagggga ctaaaagctg aggtaaagac
      661 taaagcgatt gtgccaggga tttttccgat attaaagaaa ataactgtaa gagttcctaa
      721 gatataaatg agggccataa aaggaacaac agtagttgaa accttagaaa tagacttgag
      781 tccaccaaag actgcaatcg ctacaaagac agacaagacg agagctgtga tggctggcga
      841 aatcgtcgtt gtattttgga tagattctgt aatcgagttg acttgggtga aggttccgat
      901 tcccaagaga gcaaccaata ctcctgctac tgcaaacaaa acagcaagtg gtcgccactt
      961 ttctcccatc cctagaagga tatagtgcat gggacctccc gctactgcac catggtcgtc
     1021 cttggtgcgg tatttgatgg ccaagagtcc ttccgcatac ttggtagcca ttccaaagaa
     1081 agccgccatc cacatccaaa atagagctcc tggtccacca accttgatag ccgtcgcaac
     1141 tcctatgata tttcctgttc caacagttga tgccaaggct gtacacagag ctgcaaaact
     1201 ggatacatca ccatgtccct tatcctggat aaaaataagc tgaaaggcct tgggcagacg
     1261 caaaacctgc aagagtccta gccgtatggt taggtaaatc cctgttccga ccaataaaat
     1321 caagaggggc ggtccccaag caaaagcatc gattgattta agcaattcta acatttcctt
     1381 ctcctatcgt ttcaacccca aaagaaagag cacatgcaag atacatgtac tctggaatgc
     1441 ttagataaat gctaaaaagc ggtctatcct agctctgtcc ttttacctga gagtttgagc
     1501 agttgcctgc cttgcccctt cggtgccttt acggtctctc cagagttccg tccatttaca
     1561 gtcatggaaa atcaaacgat tccccacttc tattaaactt cattcggtgt tggtatttaa
     1621 ttgattctaa ttttacaaaa aatgttggct tttgtcaatg tgtttattag taaaaattag
     1681 ttcaacagtt tttactttat aaagtccaga atactgctat cctttaaaag tgacaatagt
     1741 cgcaccactg cctccagcat tttgtggggc atagccgaaa ctcttgacat gtttgtttct
     1801 ttgcaagtat ttggtaactc cttcacggat gactcctgtt ccgataccat ggatgatatc
     1861 aacttgagcc atattgttaa gcaaggcttg gtcgatgaag gtatctagct cattcatggc
     1921 ttcttcatag cgcttgcctc gaagatccag tctagcttga ggtcctcgcc cagaagttcg
     1981 tttcacaaca ttgacctgtt tcttcttgac tggtttttct tgctgggctt gaacaagatc
     2041 aaactctttc tcttccaagg tcatcttaat caagccaact tgggcttccc agcggccgtc
     2101 cttgagttga ctggtcaagg taccacgctg accataactg agaaccacga tatcatctcc
     2161 cacctttgga gctcgttttt tcttggcctt ttgaaggacc ttgtttttag acaagtccac
     2221 tttttcagga gccaattttt tcaacttggc cttggcttca atgatttcgt ggggcttgag
     2281 ttgggattta ctgtggagat ttttgagaat ctggtcactt tcacttaggg ccatatccac
     2341 aatctcagca gcctgttcac gcgccttgtt aagctcggtt tccttttcac gattaagctc
     2401 gttgtagagt ttttttagcg cacggttcat cttgagattt tcttgctcca cctcacggat
     2461 attgtccaag cgtttgcggc tttccagcgt ctgctcttct aattgctcaa tgatacgatt
     2521 gacgtcattg tcctgatcga tctgctgact ggcatctcct acgataactt cagataggcc
     2581 tagacgtttg gcaatttcaa aggcattact tcggccagga acaccctgca taaagcgata
     2641 ggtcgggcga agagttgcag tatcaaactc catactggca ttttgcacaa aggctgtctc
     2701 aataccgtag gccttgagtt ctggatagtg ggtcgtcgcc atggtcttga tttgacgcag
     2761 gcgaaggtcc tccagaatag ccatggcaag ggctgctccc tcttggggat cagtaccagc
     2821 ccccaattca tccaaaagta agagtgaatg ttggttgacc ttgccaagaa tatccacgat
     2881 attggtcata tggctagaga aggtagacaa gctctgctca atagactgct catctccaat
     2941 atcagaaaaa atttcttcaa aaataccaac acgacttccc ttgtctgcta aaatcggcaa
     3001 tcctgactgg gccatgacct gtgtcaagcc cagagttttg agcatgatgg tcttcccacc
     3061 tgtattggga cctgtaatga caatagctgt taaatcttga ccaaaataga catcatttgc
     3121 gacggcattt ttgaccaaag gatggcagac atggagcagt tgaatctctt gattttctga
     3181 cagctgaggc acgactgctt gtctttcttg gataaatcga accttggcac gaatcaagtc
     3241 cagatgaccg ataatccaag catcattagc aatctcagcc gcatgagggc ggacacgctc
     3301 agaaatttct tggagaatgc gaagcatttc atagcgttca tctgctcgca gactagcaat
     3361 ttcttcgctc agtttgacta cctcacgtgg ttcgatatag acggtgtttc cactagcaga
     3421 aatatcatga acgacacctg caatcttatt gcggtaggtg tttttgactg gtaaaacctg
     3481 acggccattt ctgctagcaa caattccttc cgtcaacatc tgcgcttttt gcttgagcaa
     3541 gtcttgtaaa acatcgcgta cctgactctc gctatcatgt atttttcgac ggattcgcgc
     3601 caattcttca ctggcaaaat tttcaatgaa acccgcatca ttaaaggcct gaagatttcc
     3661 ttgtaattgc ggaaaatcat gtaatttctc aaaccaaagg gctaattctt ccaagctgac
     3721 attttccaga ttggcgtaaa aactttgaag ttctcggctg gcaagaagca cgcgtttcaa
     3781 gagtaggaac tcctcgatat tgagatccgc tcccatctcc aacctcttgc agactcctgc
     3841 aatttcctta gttgagagaa tagtaaaatg cggttgctcg acgaaaagag cctgcatttc
     3901 cttcatctca gcaaaagcct gtttgatttt atctgctttg gcagtcggag ccagttgtct
     3961 caattgctcc aagccctgct cggtcaacaa atgaggctca aacaaggcct tgaccttatc
     4021 gaactctaat gtttctaata ttttcttatt catctttatt tccttgtctt tgaatgtcta
     4081 ggtgtggtag ctttttacat tctgatagat tcgagtcaaa tctgtaaaca aagggctagg
     4141 aaaactcctg ccctttttat ccgattaaat ttgtcaccca gatttgtttg agccaactgg
     4201 ttgttatcgg tatgctctgg atgatgtgtt ttgcgacgat actcttttca agaggatttt
     4261 gtataactgc catggggatg gtcgccaaga ttgtcaaggc catttgcaag acaaataagg
     4321 tcaccaacat ggacaagata cctgctgaaa cttggaacaa cttaccaccc agttttttgc
     4381 taggaatcaa gtgtaagaga agaccaagca aacgaccaat gctatagaca atcccaaata
     4441 caagcaagta gccgatgcct gcataaaaga ccttatccag ctgaaagagt tgatccgatg
     4501 ggaaaaagaa agtcccctga ccttcctgcg aatttgcata agggagcaat aaatggaatt
     4561 gctctccaag ccccttataa aactggccag ccataaaagc cgatgccatg gctgaaatca
     4621 ggtaataaac ctgtaagagc aggcctctcc gatagccgat ataaaatccc caaaccaaga
     4681 ccaatagaag aaggaatgaa atcataagga atcctcaatc ttgctctgtt cttgcttaca
     4741 agtcacaagc ttgtgacgga gttcttctag ctcttgctcc ttatcgtcaa attcaatctc
     4801 acggctgagc tgagttgata aacagttgac tgccaacaaa agagcgattg tttcatcatc
     4861 tgcgctaggc atttgttctt taattgcttg gtatttttct gtcgcaacct tagcgatttc
     4921 ctccataaaa aggttgtcat gttcgcttgt caaggttaac gattttttcc cgaatgtaaa
     4981 tttgaatcga tttagatttg ccataaaatt cacctcacga tattatacca aaattcgcta
     5041 attttgtcag tttttacaaa ttttactgct tttgtggtac aatagaaact atggcaagta
     5101 tcacactcac accaagcgaa aaggatattc aggcttttct tgaacactat caaaccagtc
     5161 tggctcctag caagaatccc tatatccgct actttttgaa actacctcaa gcaacggttt
     5221 ctatctatac ttctggaaaa atcttgcttc agggtgaagg ggctgaaaaa tacgccagtt
     5281 tctttggcta tcaagctgta gagcaaacca gcggacaaaa tcttcctttg attgggacag
     5341 atgaggtagg gaatggttcc tactttggtg ggcttgcagt tgtggcttcc tttgtcacac
     5401 ctgaccagca tgccttctta cgaaaactcg gtgtgggaga ttctaagact ctgaccgacc
     5461 aaaagattcg tcagattgct cctattctca aggaaaaaat tcagcaccag gcactccttc
     5521 tctcacccag caagtacaac gaggtcatcg gagaccgcta caatgctgtt tcggttaagg
     5581 ttgccctcca taatcaggct atctatctcc tccttcaaaa aggtgttcag cctgagaaaa
     5641 ttgtgattga tgcctttacc agtgctaaaa attatgacaa gtacttggca caagagacca
     5701 atcgtttcag caatcctatc agcttagaag aaaaggctga gggcaaatac ttggctgtcg
     5761 cagtttcttc tgtcattgcg cgtgatctct ttctggaaaa tcttgaaaat ttgggacgag
     5821 aactgggtta tcagctccca agtggagctg gaacggcttc tgacaaggtg gctagccaga
     5881 ttttgcaagc ctatggtatg cagggactca gcttctgcgc caaattgcac tttaaaaaca
     5941 ctgaaaaagc gaaaaaacgc ttagaaaggt aagttatgaa ttcatttaaa aatttcttaa
     6001 aagagtgggg attgttcctc ctaattctgt cattactagc tttaagtcgt atcttttttt
     6061 ggagcaatgt tcgcgtagaa ggacattcca tggatccgac cctagcggat ggcgaaattc
     6121 tcttcgttgt aaaacacctt cctattgacc gttttgatat cgtggtggcc catgaggaag
     6181 atggcaataa ggacatcgtc aagcgcgtga ttggaatgcc tggcgacacc attcgttacg
     6241 aaaatgataa actctacatc aatgacaaag aaacggacga gccttatcta gcagactata
     6301 tcaaacgctt caaggatgac aaactccaaa gcacttactc aggcaagggc tttgaaggaa
     6361 ataaaggaac tttctttaga agtatcgctc aaaaagccca agccttcaca gttgatgtca
     6421 actacaacac caactttagc tttactgttc cagaaggaga ataccttctc ctcggagatg
     6481 accgcttggt ttcgagcgac agccgccacg taggtacctt caaagcaaaa gatatcacag
     6541 gggaagctaa attccgcttc tggccaatca cccgtatcgg aacattttaa gaaacctaag
     6601 aggccgagaa tcaccaatct cagcctcttc ttctatcgtg agaaaatgat tggtaactat
     6661 ctaaacttac cagaacagaa acacctcaac tctcacctat tcatgcaaag gaattttatg
     6721 gaagtttatt tttcaggaac cattgaacgg attatttttg aaaatcccag caatttttat
     6781 cgcatcctcc tcctagaaat cgacgatacg gacgcagagg attttgatga ttttgaaatc
     6841 attgtcacag gaaccatggc tgatgtaatt gagggcgaag actatacttt ttgggggcaa
     6901 attgtccagc actccaagta tggagaacaa ctgcaaatca gtcgttatga tcgcgcaaaa
     6961 ccaactagta agggcttggt caagtacttt tcaagtagcc atttcaaggg gattggtctc
     7021 aagacagctc agaaaatcgt ggatacctat ggcgaaaata ccattgacga aattttgcaa
     7081 cacccagaaa agttagaagg catcacagga ctctctgcca aaaatcgcga ggctttcgtc
     7141 tccactctcc gtctcaacta cggaacggag atggttttgt ccaaactagc caactacggc
     7201 attcccaaca aactagcctt tcagattcaa gacttttaca aggaagaaac ccttgatgtg
     7261 gttgaaaatt atccctacca gctggttgag gatatcaagg gtttgggctt taccattgct
     7321 gaccaactag ctgaggaact aggcatcgaa agtcaggctc ctgaacgctt ccgcgccggt
     7381 ctagttcaca gtctttttca ggcctgtatg gaaacagggg acacctatgt tgaagcacgg
     7441 gatttgctgg aacaaaccct tactctcctt gagtcttccc gtcccgtgga actagacccc
     7501 agccaagttg cccaagagct ctcctacctg atcgaagaag acaaggttca gcagattgat
     7561 accaagatct ttgacaacag cctctttttc gctgaggaag gcatccgcag tcacttggtt
     7621 cgtatccttg aaaaaggaaa acagaagagt catgatttag aaaccattca aaaacatatc
     7681 actactatcg aggaagagct gggaattgag tatgataaca ttcaaaaaca ggctatctgt
     7741 gatgctatcc agaataaggt ctttatcctg acaggtgggc ctggtactgg taagacaact
     7801 gttatcaatg gaatcattgc tgtttatgcc cttttagaag gacttgactt caggaagaaa
     7861 agcaatctgc ccattcttct tgctgctcca actggtcgag ctgctcgtcg catgaatgaa
     7921 ttgacaggtt tgcctagcgc gaccatacat cgccacttgg gaatgacagg tgacgatgat
     7981 accagtcatc tggaagatta cctagatgct gagtttatca tcgtggatga attctctatg
     8041 gtagatactt ggctggccaa tcaactcttc tccaatatct cttctaacag taagatcctc
     8101 atcgtaggtg acagtgatca gctaccttct gtcagtcctg gacaggttct agcggatctg
     8161 cttcatattc ctttgattcc tcagactcgc ttggaaaaaa tttatcgaca aagcaaagaa
     8221 tcaaccatcg tcaccctagc tagtcagatt cgacagggca tcttgccagc tgatttcacc
     8281 caaaaaaaag ctgaccgttc ctactttgaa attgctagtg gccatattcc tgccaccatt
     8341 gaaaaaatct taggtgccgc cctcagaaat ggtattcccg cccgtgatat ccaagttctg
     8401 gctcctatgt accgagggac ggcagggatt gatgctatca atcagctcat gcaagacctg
     8461 ctcaatcccc cacaaaaaga tcaactcagt tttgaagctc ctcagtgtca ctatcgtaag
     8521 agagacaagg tcattcattt ggttaacgat gctgaaatca atgtctttaa tggggattta
     8581 ggagctatca cagacctgat tcctggtaaa tacaccgaat cgaaacaaga cgagattgtc
     8641 attgattttg atggtaatga agtctcttac ccccgtaacg aatggtacaa gattcgcttg
     8701 gcctatgcca tgagtattca taagtctcag ggaagtgagt tcccagttgt catcctaccg
     8761 attactagtg ctagccgacg tatgctggag cgaaatctca tctacacagc cattacacgt
     8821 gccaaaagca aactcatctt actaggcgaa ttacaagcct tcgactatgc tacccaacac
     8881 atcggaactg cccgaaaaac ctatctgatt gaacgcttca gtgatctact ggagaatgtt
     8941 gaagaaaagc aacaagctgt ctctgaaaca gtcacatcaa gtgcctctga acaatcctac
     9001 atcctaaccg aagaaaactg ggaccgcatc ccagccatga ttgggattac agacacagac
     9061 ctcaaagaga tttttggaaa atagtgcatc agaaaaccca ccaattcaga tgggttttct
     9121 gtttattaag attattttac tgttgctgtg cttgtaatca attcaacagc ttttttgatt
     9181 gtgatatcat gtttcaacat gtcagctgaa agcaagcttt gtacttgagc aacttccatg
     9241 ttgtagtctg ctgccaattg ctcaacttct ttttggattt cttcttctga agcatcaaat
     9301 ccttcagctt tggcaactgc ttcgataaca aggttagtct tagtacgtga ctcagcttct
     9361 gcttggtatt ggttgtgaag gtcttcttga gtagttccag tgatttggaa gtacatgtca
     9421 gggttgatcc cttgacgttg caagttccca aggaattcat ttactgaacg gtgaacttct
     9481 tcgtggatca tttcttctgg aagttctacg atttcagcat tttctacagc tgtatcaatt
     9541 gctgcacctt caactgcatc tttgtaagct tcttctttag cagcagccaa ttctttgcgg
     9601 tatttttctt tcaagtcagc aagtgtttca acttcttcat caatgtcttt tgcaagttca
     9661 tcgtcaagag ctggaacttc tttagctttt acttcatgga tagttgtcac gaatttagct
     9721 tctttacctg caaggtcttc tgcttggtag tcttctggga atgttacgat aacatcaacg
     9781 gtttcgccag ctgagtgacc taccaattgg tcttcgaaac cagggatgaa ttgacctgaa
     9841 ccaagtccaa gtgagaagtt ttcacctttt ccgccgtcaa attcaacacc gtcgatagaa
     9901 ccaacgaagt cgatcacaac agtgtcgccg ttttcagcag cagcttcctt gataaccaat
     9961 tcagtcaggt tgttgcgttc gcgttcgata cgctcttcaa catcagcgtc agttacttct
    10021 ttttctacat caactgatac ttcaaggttt ttgtagtcac ccaattttac ttcaggtttt
    10081 gtaacgactt cagcagcgat aacccaatct tgaccttttt ccattgaagt tacgtcaatt
    10141 tttggttggg caaccacttc aagaccagct tcttttacag ctgcttcata agcgtttggc
    10201 aaaagagcgt tcataacgtc ttggtaaagt gattcttcac caaatttttt atcgaagata
    10261 ggacgtggaa ggtgaccttt acggaaacct ggaacattaa gagatttctt cactgacttg
    10321 aagacacggt ccaattctgg tttgatttgg tcttgagaga tagtgaaagt caagacacca
    10381 cggtttgttt ctttgttttc aaatgataca gacattctgt catttctcct taaaatttt
//
LOCUS       DAVMEI010000061        10390 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVMEI010000061 DAVMEI010000000
VERSION     DAVMEI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.061"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(51..386)
                     /locus_tag="VYQ24_001170"
     CDS             complement(51..386)
                     /locus_tag="VYQ24_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968316.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(477..1787)
                     /gene="der"
                     /locus_tag="VYQ24_001171"
     CDS             complement(477..1787)
                     /gene="der"
                     /locus_tag="VYQ24_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1968317.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(1801..2514)
                     /locus_tag="VYQ24_001172"
     CDS             complement(1801..2514)
                     /locus_tag="VYQ24_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1968318.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(2511..3407)
                     /gene="dnaI"
                     /locus_tag="VYQ24_001173"
     CDS             complement(2511..3407)
                     /gene="dnaI"
                     /locus_tag="VYQ24_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1968319.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(3408..4577)
                     /locus_tag="VYQ24_001174"
     CDS             complement(3408..4577)
                     /locus_tag="VYQ24_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1968320.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQII
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(4578..5051)
                     /gene="nrdR"
                     /locus_tag="VYQ24_001175"
     CDS             complement(4578..5051)
                     /gene="nrdR"
                     /locus_tag="VYQ24_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1968321.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5191..5556
                     /locus_tag="VYQ24_001176"
     CDS             5191..5556
                     /locus_tag="VYQ24_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1968322.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            5561..6256
                     /locus_tag="VYQ24_001177"
     CDS             5561..6256
                     /locus_tag="VYQ24_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968323.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6273..7040
                     /locus_tag="VYQ24_001178"
     CDS             6273..7040
                     /locus_tag="VYQ24_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968324.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(7452..8651)
                     /locus_tag="VYQ24_001179"
     CDS             complement(7452..8651)
                     /locus_tag="VYQ24_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968325.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(8648..9541)
                     /locus_tag="VYQ24_001180"
     CDS             complement(8648..9541)
                     /locus_tag="VYQ24_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968326.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQDQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            9657..9737
                     /locus_tag="VYQ24_001181"
                     /pseudo
     CDS             9657..9737
                     /locus_tag="VYQ24_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            10222..10390
                     /locus_tag="VYQ24_001182"
                     /pseudo
     CDS             10222..10390
                     /locus_tag="VYQ24_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3032 a         2294 c         1913 g         3151 t
ORIGIN      
        1 gattttagag gtaccataag ccacctccta caaatagaaa ccgatataaa tcaatgcctt
       61 ccacccttag acttccctag ttcctgtctc aagcgaaaca tttctttgaa acaggaataa
      121 gttaaccaat tcataccaat agctagcaga ataaaaagaa accaaatgcc ccataacttg
      181 atatctgtca catttctcaa gacggtattg aaaaacagaa ctgaaacaac tgtccaagca
      241 aggctaaaaa gagaatagaa ggggatgtaa aaccagtaaa aataataaaa aattggaaaa
      301 aacttactat ttctgttggc cttctcaatc cagttatcaa aataaaagta cggtgctaaa
      361 agtaagaatt taaacaaatg ttccatcacc gacatcaccc tccttctttt gatagcgttt
      421 tctattattt tattatatca aaaaaatccg gaactgtcat tccagattct acttttttat
      481 ttgcgttttc ttgcgatgag atgaatcggt gttccctcaa aaacaaaggc cttgcggatt
      541 tgattttcca agaaacgcag gtaagaaaag tgcatgagtt cttcttcatt gacaaagatg
      601 acaaaggttg gtggtttggt tgccacttgg gtcgcataga aaatcttgag acgttttcct
      661 ttgtctgtcg gtgttgggtt gatggcaatg gcatccatga tgacatcgtt caagacagct
      721 gatggaatac gtgtattttg actttcgctg atttgcttaa tcatctcagg aagtttgtgg
      781 agacgttgct tggttaaagc tgatacaaag ataatcggtg cgtaaggcag gtattggaac
      841 tgctcacgga tatcttcttc ccagtttttc atagtgtggt tatctttttc aagcgtatcc
      901 cacttgttga ccacgataat catcccttta ccagcttcat gggcaaatcc tgcgatacgc
      961 ttgtcgtact cacgaatgcc ttcttccgca ttgatgacca tcaagaccac atctgaacgg
     1021 tcaatagcac gcatggcacg cataacagag tatttctcag tattttcata aaccttacca
     1081 gacttacgca taccagccgt atcaatcatg gtaaactctt gaccatctgt atctgtaaag
     1141 tgggtatcaa tggcatcacg agttgttcca gcaacaggac tagcaataac acggtcttcg
     1201 cccaagatag cattgatcaa gcttgatttt ccaacgtttg gacgaccaat caggctaaac
     1261 ttaatgacat ctggattttc ttcttcatat tcatttggaa ggttttccac aatcgcatct
     1321 agtacatccc ctgtaccgat accatggaca gatgagatag gcagtggttc acccaaaccg
     1381 agggcataga aatcatagat atcatttcgc atctcagggt tgtccacctt gttaactgcg
     1441 aggataactg gtttgtgggt cttataaagc ttacgggcta cgtattcgtc tgcatcggta
     1501 attccttcct taccggacac gacaaagacg ataacatctg cttcttccat ggcaatttct
     1561 gcctggtgct tgatttgttc catgaaagga gcatcgacat catcaattcc tcctgtatca
     1621 atcatgctaa aagaacgatt gagccactca cccgttgcat aaatacggtc acgtgtcact
     1681 ccttcgacat cttctacaat ggagattcgc tcaccagcga tccgattaaa tagggttgat
     1741 ttcccaacat tgggacgtcc tacaatggca atagttggta gggccataat ttctcacttt
     1801 ctacaataat ttcttctgtt caagattttt tctagttgag cttggttcag cttgaccaaa
     1861 ctgttctgct aggcgctgac tccagcttgt ggtcgcacgc gccccagcat agtcagcctg
     1921 aacacggtca taagcttgga ttgcctcagt tgactgttct tggtattctt cctcaaagac
     1981 aacattctct agtggcagtc tcggtttcat atcatgatgt tgatttggca cacccagtgc
     2041 catcccaaag acagaatagg tgtagtcagg taggttaaag agctctgcca cttcttcaga
     2101 cttgtatcga accaaaccga taatcacacc accatagccc aagctttcag ctgccaacaa
     2161 ggcgttttgt ccagcaagag ctgcatcgac cgaactaatc aagagacctt ccacaccttg
     2221 gggttggaag gtgtcggtat gaagtcgggc tcccttttct gctcggttca aatctccgac
     2281 aaagagaagg aaaacagcag actggcgaat ggcttcttga ggtaccaatt catacaaggc
     2341 atctttcttc tcttgacttc gtaccacaat cacagagtag gattggaaat tcttccaaga
     2401 tgatgccatc tgggctgctg tcaaaatctc atttaagtct acttggggaa tttcttgctc
     2461 tttaaacctg cgcactgaag tatgagcctt catcaattta atggtttctg tcatcgacgg
     2521 tttactcctt ctaaacgagt ctcctcagcc aaataacgga tgcgttccat gacccgtctg
     2581 gcttcccagg tttcgtcatt tccatgtttc actttcgcaa aatgcttctc caaatcttca
     2641 aagttgaagt tggatgtgaa aaaggtcggt aaattttcct gcatccgata ttggagaatg
     2701 acctgcagga tttcgtcacg cacccaagcg gtggattgct cggcaccaat atcatctaaa
     2761 atcaggactt cagaaagctt aatctcatcc actaaggtct taacattgcc atcaccgata
     2821 gcatttttga catcaatgac aaagctagga tagtggagga gagttgatga aacaccacgt
     2881 ttttctgata aatcatgagc taaggccgcc accatgaaac ttttacccac accaaagtct
     2941 ccatataagt aaagaccttt tcgaatagct ggatattgct ccacgaaggc tagtagcttt
     3001 tcaaaaactg gtaagcgccc caaatcatcc aagtcaactt gagccaaact agctttcttg
     3061 agactggctg gtagattgat taacttgaga cggttcttaa tagccgcttc tttttcagcc
     3121 gcgattagct caggagtttc ttcatatgaa acatctgcat agccatgatt cttaaccaaa
     3181 atcggcttgt agcctttggc aatataatcc gtatccccac ggagaaactt gtcacgctcg
     3241 gtgatgtact gattaaactt ggagatactg cgatttaatt cctctggagt caaggattct
     3301 tgctggataa aagccgcaac atcagggtcc ttcatgattt tctggaccaa atcttgataa
     3361 taaaaacggc tgggttgacg tttgagtacg tctccgacac tttccatcta atctcctcct
     3421 ttttctaatc gagctaatag ttcttgcttc ttacgttcta gttccagacg agtttcctcg
     3481 ctggtttcat tcttatattc aggattactc catttaggaa cattggtttt tactggggca
     3541 gtctgattct gtttttgtgt ttttgctttc tgtcctcgct cacggatgcg caagactgcc
     3601 ttttctgccg aatgaatctt ttgataggca tagtcattgg ctaccttcat ggcatatttc
     3661 tcattgatat ttgccgaatc gaccttatta aaggtcaata aaagaatgat attgatgact
     3721 tcgtccagca agcccaagcc agccatctgt tgcaagagtt ctctttctgt ttgggtaatg
     3781 gttcccttgc gtgtttgctt gatttctgcc aagaactgca gggcagtttt acttttggct
     3841 tctttgataa tggtcgcttc cttaagacta aagtcagagg aaactggttt ttgagcaatt
     3901 ttttcacgca tgcgtttggt tgaaataatc tgagaaacag ctgttgactt ggccaattga
     3961 taggtttcaa accaagtcca tttcttctcc tcggcaatag caaagaggtt taagacatcg
     4021 gactgctcat ccgcaaagcg aagtccatct cgagccataa gctgacgaaa atggtccaag
     4081 tcaaaatcgt tggccacttt cttcttgaga ccaaggtctt cttgactgcc tagttctgcc
     4141 aattctggaa agacttgatt gagtgagaca ggtatttctt caccatcagc actttcaact
     4201 ttcaaatcct ccacagctac atcgccaatc tttttctcta agagtctgcg ataaacagga
     4261 tgccccaaga agtcttgact agatagagga gcatggaggg ctagctgata aacatccccc
     4321 ttttgataga gagtcaagag attaaaagca gataagattt tcaatgattt tatcagtcta
     4381 tccatcccaa agttgagatg attgaggata cttgaaaaaa gatattcctt tctaccatta
     4441 tcccaaaaac taatcgtata aagataaagg ctcagtgcct cctgaccgat aatcgggagg
     4501 tagcactgta ccagagatga ggtatcttgc gacacccgat tattctttag ataagaaaaa
     4561 cggtcaattg gcttcattta tctttccttt ttctttttag aggactgggt gatttgttgg
     4621 agcaagctct ctaactcact gacatcctta aaactacgat agacactagc aaaacgtaca
     4681 taggtaatct cgtccaattc agccaactcc tccatgacga gtgaaccaat gtcctcactt
     4741 tgaatttcat tttcatttcg accacggagt ttctgttcga tacgattgac taccatgttg
     4801 atttcatcac ttgacacagg acgtttctgg gctgagcgga taatcccatt aaagatttta
     4861 tctctggaga attgttcccg tgtgccatct tttttaacaa ccactaaggt tctttcttct
     4921 actcgttcgt aggttgtaaa acggtgttgg cattcgtcgc actcacgtct tctacgaatg
     4981 gtgttccctt cttctgcttg gcgactatcg ataacacttg acttggtagc cccacatttt
     5041 ggacaacgca tcctttccct ccttatcgtt ttcttttcat tataccattt tttaagcgat
     5101 tcccaaaaca attcttcttt ttgcttgaca agttttttgt tttgttgtat tatttagtta
     5161 agacaaaagg ataagagaaa ggagactaag atgtcctgga catttgacaa caaaaaaccc
     5221 atctatttac agattatgga gaaaatcaag cttcagattg tttcccatac actggaaccc
     5281 aatcaacaac ttccaaccgt gagggagcta gctagcgagg ctggtgtcaa tcccaatacc
     5341 atccaaagag ccttatcaga ccttgaacga gaaggatttg tctacagcaa gcgaacaact
     5401 ggacgatttg tgactaagga taaggagcta atcgctcagt cacgcaaaca attatcagaa
     5461 gaagaattgg aacacttcgt ttcctccatg atccattttg gctatgaaaa agaagaacta
     5521 ccaggcgtag tcagtgatta tattaaagga gtttaagcct atgtcattac tagtatttga
     5581 aaatgtatcc aaatcatatg gagcaacacc agcccttgaa aatgtttctc ttgacattcc
     5641 agctggaaaa attgtcggcc ttcttgggcc aaacggctca ggaaaaacaa ccctgattaa
     5701 actaattaat ggcctcttac aaccagatca aggacgtgtc ctcatcaacg acatggaccc
     5761 aagcccagca accaaggccg ttgtagctta tttgcctgat acgacctatc tcaatgagca
     5821 aatgaaggtc aaagaagccc taacctactt caagaccttc tataaagatt tcaatcttga
     5881 acgcgctcat catctacttg cagacctggg cattgatgaa aatagtcgtc tcaagaaact
     5941 atcaaaagga aacaaagaaa aggttcaact gattttggtt atgagccgtg atgctcgtct
     6001 ctatgttttg gatgaaccca ttggtggggt ggatccagca gcccgtgctt atatcctcaa
     6061 taccattatc aacaactact caccaacttc taccgttttg atttctaccc acttgatttc
     6121 tgatatcgag ccaatcttgg atgaaattgt cttcctaaaa gacggaaaag tcgtccgtca
     6181 aggaaatgta gatgatattc gctacgagtc aggtgaatcc attgaccaac tcttccgtca
     6241 ggaatttaag gcctaagcaa aggagattat ttatgttttg gaatttagtt cactacgaat
     6301 ttaaaaatgt taacaagtgg tatttagccc tctacgcagc cgtgctagtc ctttctgccc
     6361 tcatcggaat acagacacaa ggctttaaaa atctacctta ccaagaaagt caggctacta
     6421 tgctactttt tctagctaca gtctttggtg gcttgatgct tacacttgcg atttcaacca
     6481 ttttcttgat tattaaacgc ttcaaaggta gtgtctacga ccgacaaggc tatctgactt
     6541 tgaccttgcc agtttctgaa caccatatca tcacagccaa actaatcggt gcctttatct
     6601 ggtcattgat tagcaccgct gtattggctc taagtgctgt tattattctg gctttaacag
     6661 ctccagaatg gattcctctt tcttatgtga ttacatttgt agaaacacat ctccctcaga
     6721 tctttcttac aggtatatcc ttcctactaa ataccatttc aggaatcctc tgcatctacc
     6781 tggctatttc cattggacag cttttcaatg aataccgtac agcactcgct gttgcagcct
     6841 acattggtat ccaaatcgtc attggattta ttgaactttt cttcaatctt agttctaatt
     6901 tctatgtcaa ttcactggta ggactcaatg accatttcta tatgggagca ggtatagcca
     6961 ttgttaaaga actcatattc atagctatct tttatctcgg aacctactac atcttgagaa
     7021 ataaggttaa tttgctttaa ataattttta cctagatatg taacatactc atagaacaaa
     7081 agagaccagg caaaaagtct ttaaaattag aaaacgcata gtatcaagtg ttgaatatga
     7141 actgccccca aaagttagac agaaaaaatc taacttttgg ggggcagttc aaaaaatcct
     7201 tgatattatg cgttttttgt gagctgactt atttcctttc actatatcgc aaaatgaaat
     7261 aagaacggaa cgatgggatt ttggaattca aatcaattta taagaatgtt ttagaagtaa
     7321 tattatccta ttccagattc agttcactat acaattgagt tttcaagcaa cctgtttaca
     7381 taatgtgtac ataattaggt tcgtgattcc acccttttca cctttaaaaa cctcgctttc
     7441 gcaaggctct tctatttata agataaggca cgtttaaagg ttttccaaat ccctaaatca
     7501 tccgtttgaa gaacgagact agcatacatg cgtccgataa atcctgttgc taccaccgca
     7561 aaaatcactg taatagcaag tgaaatccat gcttctgctc cccccgcata gccattaatc
     7621 gttcgaaacg gcataaagaa ggtcgaaata aagggaatat aagaaccaat cttcaagagg
     7681 agattgtcac cagctgcacc tagagctgtc actccaaaaa aaccacccat aatcaaaatc
     7741 atcaaaggcg acaaggcttt ccctgagtcc tcaggacgag aaaccataga tcctaggaag
     7801 gctgccaaga ctacatacat gaaaagactg atcaaaataa agagcaaggt attcagtgag
     7861 atagcatctc ccaagtgatc caaaatacca gactgagcca agaatggcaa atctttaaag
     7921 agcaaaacgg cagccagacc acctacaaca tagatcccaa tatgcgttaa aatcactaga
     7981 aacagagcca tcatccgcgc atagaaatag tgacttgccc ttatgctaga aaaaacgact
     8041 tccataattt tggtgccttt ttcactggca acttcctgag ccgttacacc cgcataggta
     8101 atcagaatca tataaagaaa gaatcctaag gcacctgctg caattgtttg aataaacttt
     8161 ttattttcct tggcttcatc aatcttttct gtgaattgaa ttgtctgcgc taagcgtttt
     8221 tcctgctctt gagacaagga agcagttgaa cgattaagct gattttgcag ttcattgagt
     8281 gtacctgtaa cctcaaattt aattccattt tcaagcgatg tttcgccatg ataaactgcc
     8341 tttagaacac tatcttcttg atcaatggtc aaataacctt ttaatttttc ttctttaatt
     8401 gcttctttgg cacttgcttc atctttatag tcgaagttaa caccatttac attcttcagt
     8461 ccttctgcta cagatggcac tgttgtcact actgccactt tattattttt agccatagaa
     8521 gaaccttgga gatgcccaat tcctacagag attcctaaaa agaggaacgg cgaaatcacc
     8581 ataaagaaga aactccatga ctcgacatgt cgaagatagg tttccttgat tacaacccac
     8641 atatttctca tacttccact cctgattcta gtttaaagat ttcatcgata gttggcgctt
     8701 gttggtcaaa tgttgcgata tattgatctt gagtcaagat tgggaagagt tcccttccag
     8761 cgctctcatc ctccaaaatc aatttccaac tgccttgttt ggtcaagctc acctgtttga
     8821 catgaggaag attttccaat tcttccttgc ttcgttcact tgaaacaaag agacgcgttt
     8881 tcccgtattg attgcggaca tcctgaactg gtccgtgcaa gaccacacgg ccatctcgga
     8941 tcatcagaat atcgtcacaa agttcctcaa cattggtcat gacatggtca gaaaagataa
     9001 tggttgctcc gcgctctttt tcctgaaaaa tgacttgttt gagcaattct gtattaactg
     9061 ggtccaatcc actaaaaggc tcatccaaga taatcaggtc tggttcatga atcagagtaa
     9121 taatgagctg aatcttctgc tgatttcctt ttgacagact cttgatttta tctgtcagct
     9181 ttcctttcac ttccaacctc ttcatccatt gagggagttt ttctttgact tctttggcat
     9241 ccatgccttt tagagtcgcc aagtagcgaa cttgttcaag aactgtcaat ttaggcatga
     9301 gactgcgttc ttcaggcaga taaccaatcc gagcataggt ctcctgacga atatcctgac
     9361 catccagacc gatttctccc tgatattcta ggaattttaa aatactatgg aaaatcgttg
     9421 tttttccagc accatttttt ccgactagtc ccaaaatacg acctggtcgc gcttgaaagt
     9481 caataccaaa caaaacttgc ttggatccaa aacttttctc tagacttctt acttctagca
     9541 tctttcacct ccgaaatttc ttgcactcat tatactcctt tttgatagcc tttacaatgt
     9601 ttttgtccat ttttagaaga ctattgctgt gtaaaatatg gcctggagca cttttatact
     9661 caatgaaaag caaagagcaa actaggaagc tagccgtagg ctgctcaaag tacagctttg
     9721 aggttgcaga taaaactgac gaagtcagct caaaacactg ttttgaggtt gtggatagaa
     9781 ctgacgaagt cagtaactat atctacggca aggcgaagct gacgtggttt gaagagattt
     9841 tcgaagagta ttagtgataa atccattata cagtagcaaa cttaatttat accttccgct
     9901 cctcaactgt ctatttttaa tcctgaattg ttatttgagt aactcctttt tcctcataaa
     9961 gttttcttcc tctaaaactt ctggaaaaaa gcgaatagtt tcagacaaca tttttataag
    10021 aaacaagttc atctgtcatt tcaagaagga gtaatccttt atctactaat ggacggaacg
    10081 gaattcaacc gcttgtccga tatgttttct aaggattata tagtaaaatg aaataaaaac
    10141 aggacaaata gatcgggaca gtcaaatcga tttctaacaa tgttttagaa gtagatgcgt
    10201 actattctag tttcaatcta ttatatctat tatgcacacc actataggat ctaataaaaa
    10261 tcacaacaag ttcattcata gatggttacc tcagggaact aagaaaacga ctacaaagga
    10321 agtcgcattc atcgaaaagt ggattaacaa ctatcctaaa aaatgcttga actacaagtc
    10381 ccccagagaa
//
LOCUS       DAVMEI010000062        10212 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVMEI010000062 DAVMEI010000000
VERSION     DAVMEI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.062"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(83..2341)
                     /gene="glgP"
                     /locus_tag="VYQ24_001183"
     CDS             complement(83..2341)
                     /gene="glgP"
                     /locus_tag="VYQ24_001183"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1968327.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(2367..3884)
                     /gene="malQ"
                     /locus_tag="VYQ24_001184"
     CDS             complement(2367..3884)
                     /gene="malQ"
                     /locus_tag="VYQ24_001184"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1968328.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            4434..5705
                     /locus_tag="VYQ24_001185"
     CDS             4434..5705
                     /locus_tag="VYQ24_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1968329.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            5816..7108
                     /locus_tag="VYQ24_001186"
     CDS             5816..7108
                     /locus_tag="VYQ24_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1968330.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            7110..7952
                     /locus_tag="VYQ24_001187"
     CDS             7110..7952
                     /locus_tag="VYQ24_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1968331.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            8205..9005
                     /locus_tag="VYQ24_001188"
     CDS             8205..9005
                     /locus_tag="VYQ24_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1968332.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            9015..10001
                     /locus_tag="VYQ24_001189"
     CDS             9015..10001
                     /locus_tag="VYQ24_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1968333.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
BASE COUNT         2949 a         2211 c         1943 g         3109 t
ORIGIN      
        1 gaagctagcc gcaggttgct caaagcaccg ctttgaggtt gcagataaag ctgacgtggt
       61 ttgaagagat tttcgaagag tattagttca agtgccagat gtcttcatta tactgagcga
      121 ttgtacggtc agatgagaag aatcctgctt tagaaatgtt aacgatgact ttatccaacc
      181 atgcgtcacg gtcttcgtag tcagcaagca tttgctcttt gactttgatg tagtcttcca
      241 agtcaagaag agtcatgaac cagtctttgt tgatcaattc attgtaaaga cgttccaagc
      301 gctctttgtt tccagctgca agaactgcat cactaacgat gaagtcaacc aatggtttga
      361 tagcttcacg agcgtagaat tcgcttgatt tgtaagctgc ttttgcgtaa aggtcgataa
      421 cagtttctga atcttcaccg aagatgtaga tgttttcttc tccaaccaac tcagcgattt
      481 ccacgttagc accgtccata gtaccaagtg tcaaagctcc gttcaacatg aatttcatgt
      541 taccagtacc tgaagcttct ttagaagcaa gtgaaatttg ttctgagata tcacatgctg
      601 ggataaggaa acttgctgca gtaacgttgt agttttcaac cataactact tgcaagtgtg
      661 gagctactgc tggatcgtta gcaataactt ctgacatgca aaggattaaa tggataatgt
      721 cttgagcgat tgtgtaggct ggagctgctt taccaccaaa gaagattgtg attggacgag
      781 cagggatgtt accagctttg atgtcaaggt atttgtggat cacgtacaaa gcgttcattt
      841 gttggcgttt gtactcgtga agacgtttga tttggatatc aaagatagaa tttgaattga
      901 tttccacacc ttggtgttct ttcaagtgac gagccaattt acgtttgttg tgagccttga
      961 tgctttccaa tttttctttg acagctgctt tgtcttcata agacaaaagt ttttcaagct
     1021 catctgcttc atggtgccaa ccatctccaa gaatctcatc taagtagtga gacaatcttg
     1081 ggttagcatg cataagccaa cgacggaaag tgataccgtt tgttttgttg ttgaactttt
     1141 ctgggtaaag gtcgtagaag gctttcaact cagaattttt caagatttca gtatggagtg
     1201 ctgcaacccc gttaacactg tatccgtagt ggatatccat gtgagccatg tgaacacgtc
     1261 cgctctcatc gatgatttga acagctggat ctttgtactc tgccttcaca cgacggtcca
     1321 attcttcgat gattggtacc aagtgaggaa ccacttcttg caagaattca agaggccatt
     1381 tttcaagcgc ttcagcaagg attgtgtggt tagtgtaggc agtcatgcta cgaacaattg
     1441 agattgcttc gtcaagctcg ataccacgtg cagttaaaag acgaatcaat tcaggaatca
     1501 ccattgatgg gtgagtatcg ttgatttgga caactgcgta gtcagcaagg tcatgcaagt
     1561 tgcttccttt ttcgattgct tcgtcgatga tcaattgcgc accgtttgaa accatgaagt
     1621 attgttggaa gatacggagc aattcacctt gacggtcact atcatctggg taaaggaaga
     1681 gagtcaagtt gcgagcgata tctgtcttgt caaagttgat accatcttta ataatagaag
     1741 aatcaactga atccaagtca aacaaacgca agcggttttt agtcgctgtt tcataaccag
     1801 taacatcaat atcgtaaaga gttgatgtca aagtaaagtc tgcaaatggt acttggtagc
     1861 tacggcttga gcgaaccaac cagttttgct ctgtcaacca tgcatttgga attgtttctt
     1921 gttggttgtt tttaagaact tgttggaaaa gaccaaagtg gtagttaaga ccaacaccgt
     1981 caccattcaa accaagagta gcaattgagt cgataaagca ggcagccaaa cgtcccaaac
     2041 caccattacc aagagatggt tccaattcaa cttcttcaac ttcgatcaag tctttacctg
     2101 cagctgcaag ttctttttta acatcgtcgt aaagaccaag gttaatcaag ttgtttgaca
     2161 agagtttacc aatcaagaac tcagctgaga tgtagtaaac ttttttctta ccagtgttga
     2221 ctggtttttg gctgcttgca agcttgctgt agttaagaag agcaaggtaa agctcttcat
     2281 tgctacattc tgcaatggtt ttattgtaac gattttgtac aaattcttgt agtgataaca
     2341 tgtttagttg tctcctgatt attgtcttat ttctttaaat ctaccaaatt ttcattaatt
     2401 cggcgataaa ttgttgtcaa gtcaagcaaa ccttcctcga cagctggtgt caattgatct
     2461 tcagtcatac gccaagacca gtttccacca agggtagatg ggaagttcat acgagctgcc
     2521 tcatccaatt ctagtaaatc ttgcatagtt gcaattgcca taaagctaac tgatgaaaat
     2581 actgtacgaa gcatagcgtg taccactgtt tcgtattctt tacggttcgt gtaacgagcc
     2641 atgtactcac gagtcgcatc atcaatctca ttacggtacc aaccaagaac cgtattgtta
     2701 tcgtgtgttc ctgtgtacat aactgagtta gcaggtgcca agtgtgggct atcaatgctt
     2761 tcgtcttctg ggttgaaggc aaattgaaga atcttcattc ctgggaagcc agtacgttca
     2821 cgtaattcga tcacttcatc tgtcatgaag ccaaggtctt ctgcgatgat gtttagctca
     2881 ccaagttctt ccttaacggc tgcaaaaagc ttgtagcctg gacctttcac ccactcacca
     2941 ggtgctgctg tatcggaacc agcagggatt tcccaataag attcgaagcc acggaagtgg
     3001 tcgatacgaa cgatatcgta gattttgaag ctttcacgca agcgttcaat ccaccatttg
     3061 tagccgtctt tgtccattgc ttcccagtca tagattgggt taccccaaag ctgaccagtt
     3121 acagaaaact catctggtgg gcatcctgcg atacaagtag ccttaccatt gacatctgtt
     3181 ttgaagagat gtggatttgc ccacatatca cttgaatctt ccgctacgta gattggcatg
     3241 tccccaacga tttcaatgtg gttgtcgtta gcgtaagctt tcaatttcaa ccattgttgg
     3301 aagaagaagt attgagtcac acggtggtaa accaacttgt ctgccaattg ctcacgatag
     3361 ctttcaagtg ctgaagcttt acgagcacga gcatctgcat ctggccattc agtccaagca
     3421 agattgtcaa aatgctcttt gatagccata tactcagcaa agagctcaag ccatgattgg
     3481 ttgtcttgag caaatttctc aaaatcttta acatctccga cttcaaagaa acgtttcacc
     3541 gctttttcta aaagaggacg acgtgcatag tagattttag catagtcaac ttcagacgca
     3601 tcgctaccaa agtcaactcc ttcaaggtca ctcgctccca acaaaccttg ctccaccaag
     3661 atatctaaat cgataaaatg agtgtttcct gcgaaggctg agaaagattg gtaaggagaa
     3721 tccccgtaac tagttgctcc taatggaagg atttgccagt aacgttgttt tgtacggacc
     3781 aagaaatcaa cgaagtcgta agcactttga ccaaatgatc cgattccgta agctcctgga
     3841 agagaagaga tgtgcatcaa cacaccactt tgacgttttt tcataatagc acctcgtgtg
     3901 ttaaaataat ggaacgttgc gtattttgca gacgcaaacg tttgcgttac ttataagtat
     3961 actccttttc aagcataatt gcaagcgttt ttaaaaaact tttttgatta tttttaagga
     4021 gactaactca ttttagcaaa gataacttaa aactatctaa tcctaaagtt tgactctcac
     4081 gagacagtct acaaagtaca aaacctcctt agggagcttg aatttatatg caacaaagca
     4141 caaacgtcta tagaaaatag atagggatta gaaacaggga ggtagcccct cctgatttcc
     4201 ctctttagac agattccata tatttttatt aaaaatactg tattcttatc tatttcatac
     4261 ttttcttcta ttaaaatgca aaattatgct atcaaatcaa gcttaaaaat tttttaaaat
     4321 ttttacaaaa aatacttgca accgttttct atttgtgcta tactaagctc ataaaggaaa
     4381 acgtttgcgt ttccttatca ataaaacttg ctattctttg ggaggaatac actatgtcat
     4441 ctaaatttat gaagagcact gcggtgcttg gaactgttac acttgctagc ttgcttttgg
     4501 tagcttgcgg aagcaaaact gctgataagc ctgctgattc tggttcatct gaagtcaaag
     4561 aactcactgt atatgtagac gagggatata agagctatat tgaagaggtt gctaaagctt
     4621 atgaaaaaga agctggagta aaagtcactc ttaaaactgg tgatgctcta ggaggtcttg
     4681 ataaactttc tcttgacaac caatctggta atgtccctga tgttatgatg gctccatacg
     4741 accgtgtagg tagccttggt tctgacggac aactttcaga agtgaaattg agcgatggtg
     4801 ctaaaacaga cgacacaact aaatctcttg taacagctgc taatggtaaa gtttacggtg
     4861 ctcctgccgt tatcgagtca cttgttatgt actacaacaa agacttggtg aaagatgctc
     4921 caaaaacatt tgctgacttg gaaaaccttg ctaaagatag caaatacgca ttcgctggtg
     4981 aagatggtaa aactactgcc ttcctagctg actggacaaa cttctactat acatatggac
     5041 ttcttgccgg taacggtgct tacgtctttg gccaaaacgg taaagacgct aaagacatcg
     5101 gtcttgcaaa cgacggttct atcgcaggta tcaactacgc taaatcttgg tacgaaaaat
     5161 ggcctaaagg tatgcaagat acagaaggtg ctggaaactt aatccaaact caattccaag
     5221 aaggtaaaac agctgctatc atcgacggac cttggaaagc tcaagccttt aaagatgcta
     5281 aagtaaacta cggagttgca actatcccaa ctcttccaaa tggaaaagaa tatgctgcat
     5341 tcggtggtgg taaagcttgg gtcattcctc aagccgttaa gaaccttgaa gcttctcaaa
     5401 aatttgtaga cttccttgtt gcaactgaac aacaaaaagt attatatgat aagactaacg
     5461 aaatcccagc taatactgag gctcgttcat acgctgaagg taaaaacgat gagttgacaa
     5521 cagctgttat caaacagttc aagaacactc aaccactgcc aaacatctct caaatgtctg
     5581 cagtttggga tccagcgaaa aatatgctct ttgatgctgt aagtggtcaa aaagatgcta
     5641 aaacagctgc taacgatgct gtaacattga tcaaagaaac aatcaaacaa aaatttggtg
     5701 aataaaaaat ttgttcaagg ggggtggaaa tcaaatcccc ctttgaattt atcaatagag
     5761 acacaaataa tttagctttc ttataaaaaa gtagtatcct atgaaaggag ttaatatgga
     5821 aaagcaacaa cctagtaaag cagccctgct gtctatcatt cctgggttag gacagattta
     5881 caataaacaa aaagccaaag gttttatctt ccttggtgta accatcgtat ttgtccttta
     5941 cttcctagca cttgcaaccc ctgaattgag caacctcatc actcttggtg acaaaccagg
     6001 tcgtgataat tccctcttta tgctgattcg tggtgccttc catctaatct ttgtaatcgt
     6061 ttatgtactc ttttatttct caaatatcaa agatgcacat acgattgcaa aacgcattaa
     6121 caatggaatt ccagttccac gcacactcaa agacatgatc aaagggattt atgaaaatgg
     6181 cttcccttac ctcttgatca ttccatctta tgttgccatg accttcgcga ttatcttccc
     6241 agttatcgta accttgatga tcgcctttac caactacgac ttccaacact tgccaccaaa
     6301 caagttgttg gactgggttg gtttgaccaa ctttacaaac atttggagct tgagtacctt
     6361 ccgttctgcc tttggttctg ttctttcttg gactatcatt tgggctttgg cagcttctac
     6421 tttacaaatc gtaattggta tcttcacagc tatcattgcc aaccaaccat ttatcaaagg
     6481 aaaacgtatc tttggtgtta ttttccttct tccttgggct gtcccagcct tcatcactat
     6541 cttgacattc tcaaatatgt ttaacgatag tgtcggtgct atcaacactc aagtattgcc
     6601 aatcttggct aaattccttc ctttccttga tggagctctt attccttgga aaacagaccc
     6661 aacttggact aagattgcct tgattatgat gcaaggttgg ctcggattcc catacatcta
     6721 cgttttgact ttgggtatct tgcaatctat tcctaacgac ctttacgaag cagcttatat
     6781 tgacggtgcc aacgcttggc aaaaattccg caacatcact ttcccaatga ttttggctgt
     6841 tgcggcacct actttgatta gccaatacac cttcaacttt aacaacttct ctatcatgta
     6901 cctcttcaat ggtggaggac ctggtagtgt cggaggtgga gctggttcaa ccgatatctt
     6961 gatctcatgg atctaccgtt tgacaacagg tacatctcct caatactcaa tggcggcagc
     7021 tgttaccttg attatctcta tcattgtcat ctcaatctct atgatcgcat tcaagaaact
     7081 acacgcattt gatatggagg acgtctaaga tgaataactc aattaaactc aaacgtagac
     7141 tgactcaaag ccttacttac ctttacctga ttggtctatc aattgtaatt atctatccac
     7201 tgttgattac cattatgtca gcctttaaag caggtaacgt ctcagccttt aaactagata
     7261 ctaatatcga cctcaatttt gataacttta aaggcctctt cactgaaacc ttgtacggta
     7321 cttggtacct caacactttg attatcgcct taattaccat ggctgttcaa acaagtatca
     7381 tcgtacttgc tggttatgct tacagccgtt acaacttctt ggctcgtaaa caaagtttgg
     7441 tcttcttctt gatcatccaa atggtgccaa ctatggccgc tttgacagcc ttcttcgtta
     7501 tggcgcttat gttgaacgcc cttaaccaca actggttcct catcttcctc tacgttggtg
     7561 gtggtatccc gatgaatgct tggctcatga aaggctactt cgatacagtg ccaatgtctt
     7621 tagacgaatc tgcaaaacta gacggtgcag gacacttccg ccgcttctgg caaattgttc
     7681 taccacttgt tcgcccaatg gttgccgtac aagctctctg ggccttcatg ggacctttcg
     7741 gggactacat cctctctagt ttcttgcttc gtgagaaaga atactttact gttgccgtag
     7801 gtctccaaac cttcgttaac aatgcgaaaa acttgaagat tgcctacttc tcagcaggtg
     7861 ctatcctcat cgcccttcca atctgtattc tcttcttctt cctacaaaag aactttgttt
     7921 caggacttac aagtggtggc gacaagggat aatttatccc cgccaccctt tttcatttta
     7981 tactcttcga aaatctcttc aaaccacgtc agctttatct ccaacctcaa agttgtgctt
     8041 tgagcaacct gtggctagtt tgcactttga ttttcattga ttattagcaa ttgtcactgt
     8101 aaataatatc cttgtagcaa gcaatttttc tcctagactt gaaataaagc gcatttctct
     8161 atataataat actcatatag aaaacacctt ttagaaagat acctatgctt ccatatccat
     8221 tttcctattt ttcaagtatt tgggggtttc gtaagcccct gtccaaacgt ttcgagctca
     8281 actggtttca acttctcttt accagtatct tccttatcag cttgtctatg gtacccattg
     8341 ctatccaaaa cagctcccag gagacctatc cgctagaaac ttttatcgat aatgtctatg
     8401 aacctctgac aaatgaggtt gtccaggatc tctctgaaca tgctacaatt gtcgatggca
     8461 cattaactta tactggaaca gctagtcaag ccccttctgt tgtgattggt ccaagtcaaa
     8521 tcaaggaatt acctaaggac ttgcaactgc atttcgatac aaatgagcta gtcatcagca
     8581 aggaaagcaa ggaactgacc cgcatctctt accgagccat tcagactgag agtttcaaaa
     8641 gcaaagacag cttgacccaa gcaatttcta aagactggta ccaacaaaat cgtgtctata
     8701 tcagcctctt cctagttctc ggtgcgagct tcctctttgg tttgaatttc tttatcgtct
     8761 ctctaggagc tagtcttctc ctttatatca ctaagaaatc acgcctcttt tcatttagaa
     8821 cctttaaaga gtgctatcac tttatcttga actgtttagg attgccgact ctgattacac
     8881 ttattttggg attatttggc caaaatatga caaccctgat tactgtacaa aatattcttt
     8941 ttgttctgta tctggtcact atcttttata aaacacattt ccgtgatcca aattaccata
     9001 aataggagat ttttatgccc gttacgatta aagacgtggc caaggctgct ggtgtttcgc
     9061 cttcaaccgt aacccgtgtt attcaaaata aatcaaccat tagcgacgaa acaaaaaaac
     9121 gcgttcgcaa agctatgaag gaactcaact accacccaaa cctcaacgct cgtagcttgg
     9181 taagcagcta tactcaggtt atcggattag ttcttcctga tgactcagac gccttctacc
     9241 agaatccttt ctttccatcg gttctacgtg gcatctctca agtcgcatct gaaaaccact
     9301 atgccattca gatagcaaca gggaaagatg agaaggagcg tctcaacgct atttcacaaa
     9361 tggtctacgg caagcgtgta gatgggctaa tctttctcta tgcccaagaa gaagaccctc
     9421 tcgtaaaact cgtcgcagaa gaacagttcc ccttccttat cttaggtaaa tctctatctc
     9481 ctttcatccc acttgtcgac aacgacaatg ttcaagctgg ttttgatgcg actgaatatt
     9541 tcatcaaaaa aggctgcaaa cgcattgcct ttatcggagg aagtaaaaag ctcttcgtga
     9601 ccaaagaccg tttaacaggc tatgaacagg cgcttaaaca ttacaaactt accactgaca
     9661 acaatcgcat ctactttgcc gacgagtttc tggaagaaaa gggctataaa tttagcaagc
     9721 gattattcaa gcacgatcca caaattgatg ctatcatcac aaccgatagc ctcctagctg
     9781 aaggtgtttg taactatatt gccaaacacc agctggatgt ccctgttctc agctttgact
     9841 cggttaatcc caagctcaac ttggcagcct atgtcgatat caatagttta gagcttggtc
     9901 gtgtttccct tgaaactatt ctccagatta ttaatgataa taaaaacaat aaacaaattt
     9961 gttaccgtca attgatcgcc cacaaaatta tcgaaaaata agagactggg caaaaagtcg
    10021 ttaaaagcaa aaacgcatag tatcaggtat tgaaaaaact tgatactatg cgttttaatg
    10081 tgggaagatt tacttccttt tctactgaaa ttgagtcttt tcccaagatc tttttatact
    10141 caatgaaaat caaagtgcaa actaggaagc tagccgcagg ttgctcaaaa catggttttg
    10201 aggttgcaga ta
//
LOCUS       DAVMEI010000063        10202 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVMEI010000063 DAVMEI010000000
VERSION     DAVMEI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.063"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(87..1301)
                     /gene="thiI"
                     /locus_tag="VYQ24_001190"
     CDS             complement(87..1301)
                     /gene="thiI"
                     /locus_tag="VYQ24_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1968334.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(1310..2452)
                     /locus_tag="VYQ24_001191"
     CDS             complement(1310..2452)
                     /locus_tag="VYQ24_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1968335.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            2590..3018
                     /locus_tag="VYQ24_001192"
     CDS             2590..3018
                     /locus_tag="VYQ24_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968336.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            complement(3169..5394)
                     /locus_tag="VYQ24_001193"
     CDS             complement(3169..5394)
                     /locus_tag="VYQ24_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1968337.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(5558..7510)
                     /locus_tag="VYQ24_001194"
     CDS             complement(5558..7510)
                     /locus_tag="VYQ24_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1968338.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHSYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQVP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            complement(7507..8418)
                     /gene="pfkB"
                     /locus_tag="VYQ24_001195"
     CDS             complement(7507..8418)
                     /gene="pfkB"
                     /locus_tag="VYQ24_001195"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1968339.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            complement(8415..9155)
                     /locus_tag="VYQ24_001196"
     CDS             complement(8415..9155)
                     /locus_tag="VYQ24_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1968340.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            9317..9404
                     /locus_tag="VYQ24_001197"
     tRNA            9317..9404
                     /locus_tag="VYQ24_001197"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9351..9353,aa:Ser,seq:gga)
     gene            complement(9587..9934)
                     /gene="ssrA"
                     /locus_tag="VYQ24_001198"
     tmRNA           complement(9587..9934)
                     /gene="ssrA"
                     /locus_tag="VYQ24_001198"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2986 a         2316 c         1953 g         2947 t
ORIGIN      
        1 aataaaaaaa gttagttttt tacctgtttt tagagaaaaa actaactttt tttactgatt
       61 ttcgctattc ttttggattt tctgaattag agcagattgt caatcaagtc atcaacttca
      121 tctttttcgg cttgaggtgt gatttcagta atcatgattc cagccactgc tcgctcaacc
      181 aagccttcaa catccatacg cgcttcgtac tgctccgcat tcttaatttt aggatttgtt
      241 tttggacgat ctggtgcaaa aatagtacaa cagtcttcaa acggttggat tgaaatgtca
      301 aaggtatcga tttcctgggc gatgtcaatg atttccaact tgtccatggt aaccacagga
      361 cgaatgatgg gagtgttggt aacagcattg atagccttca tactttcaag ggtttggctg
      421 gctacttgac ctagactttc cccattgatg ataactaaac catttcgtac ctcacgaata
      481 cggtcagtaa tacgcatcat aaaacgacgc gtcaaggtca taaggtaggc ttctggcgct
      541 ttggctttga tttcctcttg aatctctgta aaaggaactt cgataaactg gatatttccc
      601 ccaaatttgg ttaatttacg ggtcaaatct tgggcttttt taagagcacc aggactcgtg
      661 taaggtgggc tggcaaagtg aactgcctct atatctaccc ctcgtttaag cgctagatag
      721 ccagctacag gggaatcgat tccccctgac aacatgagca tgcccttacc agaagttccc
      781 actggtaatc cacctgcccc tcgaacggtt tcataagaaa gataggctgc ttcttcacgt
      841 atctccacct gaagattgat gtcaggactt ttcatttgag cttgtacgtt tggaatggct
      901 tcgaatacag cccctccaag cgtttggttg agttcacgac tgtccaattc aaaggtgtgg
      961 tcgctacgct tgctagagat tttaaaggtc atgccttcct tgtagatgtc ctgcataatc
     1021 tcttggacag cagacttcag aacttctaca gatttttcaa ccttataaac aggggaaaag
     1081 ttttgaattc caaatacttg tttgagcgat tctgctactg ctgtgtaatc tgctccattg
     1141 aggtaagcgt gggcacggtc gcgatctgct gttaccttaa cttggggata gatagacaaa
     1201 acgtctgaaa tattattgcg aagtttattg atgaaacgca tacgattttt gcccttggtt
     1261 gacagctcac cgtagcgaat cataatttct gaatactgca tgaatgctcc tatcttactt
     1321 ttctagtttg attgtaaatt aattttagct tggtcaagaa ctgctcgacc tgactcatat
     1381 cattttcaag gtctagacta agacgcacag ctgactgagc cttatccttg tccactccca
     1441 tagcaatcag ggttccggct ggtttcccag ccttggacga acaagccgag gtcgttgaga
     1501 tgaaaatatc atagtcttca aaggcgtgaa caatgacctc accacgaaca cccttgattc
     1561 caaaagtcag aatatgaggg gcgaagtctt cctcatctga aaagacaaat atatccggat
     1621 agtccagaag tgcttgacga atcactgcct tcatctgccc agtcttgctc ctaaagatat
     1681 ctagcttttc catagataaa cggagagcct tagcggtcgc tgcaattcct gccacatttt
     1741 cagttgtcga acggtaatca cgttcctgac caccacctgt taaaagaggc gtaatcttct
     1801 tgccagactt gatatagata aaaccaacac cacggattcc gtggaacttg tgaccagaga
     1861 aagtcgcgaa atccactcgt tctgtcagat acttttcagt cggaatcttg gcaagcgcct
     1921 gaaccgcatc aacgtggaag gaaatagttg gcttgtctgc caagaattct gaaatagcct
     1981 caataggttg gatagagccg atttcattgt tcacagccat gatggaaacg agggtcgtat
     2041 caggtcttat caaatcttct agcgcctcaa catccacaaa tcctttgtta tcaactggag
     2101 caaaatccac ttcaaaacct tgagctttca accagagggc tgattcctta actgctggat
     2161 gttcaatggc tgatacgatg atgtgcttgc caaactgggc tttttcaaat gctactccct
     2221 tgatgatcca gttatcccct tctgtcccac cagatgtaaa gaagatttca tcacttttct
     2281 taccgattaa atctgcaatc tgttgacggg aagcatctaa aattcgtgtt gcctggtctc
     2341 ccaaacgatg gagactagat ggatttccta aaatttttga agccacctgc atataggttt
     2401 caagggcttc tggatagggc ctggtcgtcg ccgaattatc aaagtaaatc atgtttctca
     2461 cgctttctaa aatcactcct tctattgtat catgaaacgg agcctgcgac aagaaagggc
     2521 aatttctctt tttttaagat ttttttaaag aaacagagta taataaatca taatcaaagg
     2581 agagaatgta tgtctaacta tcgtagaact tcaaaaccga aaacagaaca tatcaaaaaa
     2641 ggctttactg tttttcaaaa aaccattact actatcggta gtatccttgg tttgattacc
     2701 gcgggtatca cgatcatgaa cgccttggat aataataata aaaatactaa aaaagaacct
     2761 acgacaagcc agacgacaac ctttgtcaaa gaaattcaaa aagaatcccc tcaggaaaat
     2821 actactccaa ataaggaaaa taacacttct caagaaaaaa cacaacaaga agaaacgcca
     2881 aaatctagcg tcaaggaaga gaaaaaagaa gatcagaaaa cagcaactca ggactctact
     2941 acacctgcta caagtaaacc tgccactgaa aatgaaaaac agcccaatac tccaacttca
     3001 gaaaataata ctcaatgaaa atcaaagagc aaactaggaa gctagccgta gacagtactt
     3061 gagtacggca aggcgaagct gacgtggttt gaagagattt gcgaagagta taaaagtaat
     3121 caatagccag taaaatagct ccttccaacc ttggaaagaa gctatttttt attgctgcaa
     3181 tacttttctt ggcttggtac cttcagctgg accaatgaca cctgccatct caagctcttc
     3241 catgagacgg gtcgcacggt taaatccaac tgacaaacga cgctgaatca tggatgcact
     3301 ggctttctgt gtttcgataa ccaaagactt agcttcttca aaaagcggat caccaccagc
     3361 atctccatcc gaaaattctc cttcattttc agaaacctca cctggatcaa aactctcatc
     3421 gtagtctgca tctgcctgag tcttgatgaa gttcacaatg cgctcaacat cgtcatccga
     3481 gataaaggag ccttggagac gaactggatg attttcatca atcggtttaa agagcatgtc
     3541 tcctcgacca agaagttttt ctgctccatt ttcatccaaa atcgtacggg agtctgttcc
     3601 tgatgaaacc gcaaatgcta cacgagatgg aacattggcc ttaatcaaac cagagatgac
     3661 atcaacagat ggacgctgag ttgcaagaat catgtggata cctgcagcac gcgccttctg
     3721 cccaagacgg atgatagcat cttccacttc cttgctggcc accatcatga ggtcagccaa
     3781 ctcatccaca atcacgacaa tgaatggtag cggaatttgc ttgtactcag actgggaatt
     3841 gaactcttct accttggcat taaaacctgc aatattccga actcccacct tggcaaagag
     3901 ttcataacgg ttttccattt catccacaac cttttgcaga gccttgctgg ctttgcgtgg
     3961 attggtcacg actggaatca agaggtgggg aatatcattg taaacagata actcaaccat
     4021 cttgggatcg accatcataa atttaacttg atctggtctc gccttcatga gaatgctagc
     4081 aataatgccg ttaactgcta ctgacttccc tgaacccgtt gaacctgcaa ctagcaagtg
     4141 gggcatttta gaaaggtcaa aagctcttgc ggttccatta acagccttcc ctaaaggaat
     4201 ttccaagaaa ttttctgctt tcgtttgcga ttgttcccat agttctcgga aagatacagt
     4261 ggcaatatcg gagttgggca cttcaattcc gattagggat ttcccaggga ttggtgcttc
     4321 aatccggaca tctttggcag ccaaggctag agcgaggtca tctgatagat tggaaatgcg
     4381 gttgaccctt acaccaacag ccggcttgac ttcatacttg gtcactgatg gcccaatttc
     4441 ggcccgttca actgttacct taataccaaa gctagcaaag gttgcttcta agattttgat
     4501 attttctctg acaattttct tctctttaga ctgatctttt ggtttatctg gtgcaaagag
     4561 ttgtaagctt ggaagtttgt attcaagggc ttctttggct gaaaaatcga cctgaacatc
     4621 ttcgtcatct gagtcatctt cctgttcagg gaatttaagt tcagcttgag gcaggatgat
     4681 ttctggttcc acccactttt cttctggaat aggtggaaga ttttgaacag cttcctctgt
     4741 cagaatttca cccgtttcca tatcaacagg aggcaaatcg agtaaggctt tttcagtctc
     4801 ttcttgttct aatctagcct ctttctcagc cttttggcga gctttttctt cttgtttgac
     4861 aaagcgttcc tcttttcgac gctcgtgccc ttcccaccat ttggcaaagc ctctactgaa
     4921 aaattcagca atatcgtaaa cagaccaagg gctgactagg agagaaccca ctaaaatcaa
     4981 gatagaacca ataaagtaag ttccgatatt tgaaaagaga aaagctgttg gaatataaag
     5041 agcgaccccg atcaagcccc ctccagcaaa actagtcgtt cgaaaaccag tcagatctgt
     5101 cacaacctga gccatggtcc cttttagaac ggacttgtcc aaaccatatt tccaaaccaa
     5161 gtaggcttca aaaatcaaga gtaagccagc aaatatggtg aaaaagccag ataagagtcc
     5221 ttcctgtttt cgtatccact tgaaaaaaaa gagatagatt aataggccga atatcgccag
     5281 ataagctagg ctacccacta gcaagcgaat taaattataa agggttatac ctgcagcccc
     5341 taatttgaag gctgcgaaaa tcaataaaat cgcaattcct aacgaaatca acattcgttg
     5401 aatcgcttct tttctttcca gttctgcttt agacggtctc cgtcttgttg tacttgtatt
     5461 cttgtttgcc attcttctat tataccatat ttcacaggca tttcggaaag agaaaaagac
     5521 tgcaccaaac ggtccaatct tttcattttt ctatttttta tgcttgtggt ttgcgtaggt
     5581 aaccataaac cacaccactt acgattgctc ctaccaagac agaaacgagg taaaggagag
     5641 catttgaagt aagggcgata acgaagattc ctccgtgtgg cgccatgagt ttaataccag
     5701 taagaccaac gagtccacct gctactgctg aaccaaggat gaagcttgga atcgcacgag
     5761 ctgggtcagc ggcaccaaat ggaatcgctc cctcagtgat aaatgacaag cccatgatga
     5821 tgtttgtcaa accagagtta cgttcttcct tagtaaattt atctttgaaa agaagagttg
     5881 cgacaaagat tgcaagtggt ggcaccattc ctccagccat aactgctgcc atggctacag
     5941 aaccacctga agaaacagtt gctgcaagcg tacctgtacc aaagacataa gctgctttat
     6001 taactggtcc acccatgtca acagccatca ttccaccaag gacgatacca agaaggacag
     6061 ctgaacctcc tccaagaccg cctaggaagt cattcatagc agtgttgatt gctgccattg
     6121 ggatattcac agctagcata acaaatcctg tcaagattgt tccaagaagt ggcaatagaa
     6181 ggattgattt agcaccttcg agtgaacgag gaactttaac gtatttcttg atggcaagaa
     6241 ccaaggcacc tgcgataaat ccaccaacaa gggcacctag gaaaccagat gagacacctg
     6301 caagagttga agttgcttca ccacctgcgg cataaggaat tttaccaaag gcaaaacctt
     6361 ctttggcaat agcaccagcc acgaaacctg ctaccaaacc tggtttttca gcaatagagt
     6421 aggcaacata acccgcaaag actggaagca tcaaaccaaa ggcagctcca ccaattttca
     6481 tgaacataga agctaactca tggtaagaac caagattgcc aaggttttca tttggaacac
     6541 ccaaagcacc gtcaatcaag aaggcaaggg caatcatgat accaccaccg ataacgaatg
     6601 gtaacatttg agatacacca ctcatcaagt gtttgtagaa ggcaccacca aggctttgtt
     6661 tttcgttaga ggctgttgca gcttttgcac cattagcggc acggtagact tcagtatctc
     6721 ctgaaagagc caagttaatt agctcttctg tcttacggat accgtcagca actggacgat
     6781 tgatcaatgg ttttccatca aatcgatcca tttcaacggc cttgtctgct gcaatgataa
     6841 tagctttagc cttacggata tcttctgcag ttagttgatt tccaacaccg ctagcaccgt
     6901 tggtttcgac cttgatacca acccccattt cagcagctac tttttgaagg gcttcttggg
     6961 ccatgtaagt gtgggcaata cctgttgtac aagctgtaac agctacgata aagtcaccag
     7021 agtcattagc aggtacttga acaagttcct cagttttttc tgaagcttgg tcaaaaagtt
     7081 cgataacttg gtctgcagat gttgcttgac gaagtttgtc tgcaaaaccg tctttcatca
     7141 agtattgaga caattctgcc aaggctgcca agtgagtatc attggcacct tctggagctg
     7201 caatcatgaa gaagaggtca gttgcttgtc catccaagct ctcgtagtca acacccttat
     7261 ttgacttagc aaatagaact gtcgcttctt tgacagcagc gtttttgctg tgaggcattg
     7321 cgattccatc acccaaacca gtagaagtca aagcttcacg cgccaaaatt ccttctttaa
     7381 atgtttcaaa atctgttaca taactgtgct ctgtcaaatt tttaatcatc tcgtcgatga
     7441 cagctgtttt ttcagttgcc tgcaaatcta gcaacatgac atcttttctc aataggtctt
     7501 gaattttcat cttttttcta cctcaacttt tccatatgtt tctttaataa attccgccgt
     7561 tgccaagtca tctgagaagg tagttgccgt tccgcaagcc actccccatt tgaaggcttc
     7621 tactgcgtct tttgatttga caaattcacc tgtgaatcca gcaaccatag aatcaccagc
     7681 tccaactgaa tttttgactg ttcctttgat tggtttagcg aagtaagctc cctcagatgt
     7741 gacaagaagg gcgccatcac cagccataga gataataaca ttttgagcac ccttagccag
     7801 taactcacga gcgtatttct caatttcatc taaactttcg agtttaaccc caaaaatcgc
     7861 tccaagttca tgattgtttg gttttacaag aagaggctgg tagtccaaac tatcaattaa
     7921 ggtctgtcct tcaaagtcac agaccacttg cgcaccagtc tggcgcgtca aggaaatcaa
     7981 atccttatag ataacattgc ctagattttt agcacttgaa cctgcaaaga caactgtatc
     8041 ttctgctgtc agactagata aaatagcttt caattcttct agctgaaccg gttcaacagt
     8101 tggacccgtt ccgttgattt ctgtttcttg gtctgctttg attttaacat tgatacgagt
     8161 atcttctgcc acctggacaa aacgtgtctc gatttcttcc tctgctaaag tatctgtgat
     8221 aaatttacca gtaaagccac cgataaatcc cgtcgctgta tttggtatat tcaaacgttt
     8281 caagacacgg ctgacattga ttcctttccc accagcaaac ttatcatcac tgtccatacg
     8341 atttacacta ccaactttga cttggtccaa acgaacgata tagtcaatgg atggattgag
     8401 tgtgactgta taaatcatac ttctattacc tccgttttct ccttaataac ctgcaagagc
     8461 tcatgccctt gactagtgat aacgatagcg cgtttgagtg gtgctacctt ggcaaagcaa
     8521 gtttgtccaa tttttgacga atccaccaag acgtaggtct gcttggcatt ctccaaaata
     8581 gctcttttca cagctccttc ctccatatca ggagtcgtat aatagccatc gtcaacacca
     8641 ttcattccga taaaggcacg gtcaaagtgc aattggttaa tctggttaag agcaacgcca
     8701 ccgatactag catctgtcgc cgtcttgacg tttcctccaa ccatgacagt tggaatctgc
     8761 ttttcaacca actgagcggc atggtgaatg gagttggtca caactgtaat attcttattg
     8821 accaattcat gaatcaaaaa agcagttgtt gttccagcat cgataaagat gacatctttt
     8881 tctttaatga gagaggctgc tttctgagcc agcaacttct tttcttgaag gtttttgaca
     8941 gatttttctt gaatggtttc ttcttcctgt aaggaatggg ggagttctgc tccaccatgc
     9001 acacgacgaa gcttgttttc cgcttccaac tcatccaagt ctcttcgaac cgttgattct
     9061 gacgtttcta gcaaactaac taatttttct agagaaacta catgatgttg atttaactcc
     9121 tctaaaatca gttgcttccg ctctgttttt aacaccgaat cacctcctgt tatcgtttac
     9181 actattcatt ctatcatact ttcttttgaa gtcaagcatt ttttatcatt ttctttcaaa
     9241 atctatcatt tttcttattt ctagaatttt tattgcaaga taagagcctt tatggtagac
     9301 tatttgagta agtattggaa gattactcaa gaggcttaag aggccgtgtt ggaaacgcgg
     9361 taggcgtgta atagcgtgcg tgggttcgaa tcccatgtct tccgtcgaaa aaagattatt
     9421 tttaggatgc gacaaatgtt gtatcctttt ttctatcctt tttttaaaat agggtctgtt
     9481 tgtgactccc tttttagcaa aagaatgcaa atgaatacca aagataaaaa taaaaaacgt
     9541 tgatttaaca acgttttacc aaggaatgca aaagaatgca aaggaataat ggagccggtg
     9601 ggagtcgaac ccacgtccaa acacctgcca acatatttgt ctacaaccat aggttatgta
     9661 ttattttaac agcccctcga cacataactc aagtctagaa actgcgagtc tatcaatctc
     9721 ttatcaaacc gctagacaag gcttaatcgt atctcgctaa taataagacc tgtcattgaa
     9781 cacgagcaat ccaaatcggg tcacgcctgc tggtttttag gcagctagag cgtaagaagt
     9841 gttatttttt gcagttatat ttaactgagc gtttacgtcg ccacacgagt cgcaaaatat
     9901 gcctcataat gcctgtcgaa tccgtaacga ccccaaaaga caataaaacc attatacctt
     9961 atctaactca aaaatgcaaa aagaaatcac atacctaaaa aagatactat tcgagaaatt
    10021 tagcaaactg gaagactaac tacaactttc tcagtgcata actttacgat tgtaaataga
    10081 gcttacgaaa tccaattaac acatacctaa gatgaagcta tctgttttga agaaattcac
    10141 gaagagaatt attctaaaat actttcagat gagaattttc gtactctctt tattttaatt
    10201 ca
//
LOCUS       DAVMEI010000064        10012 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVMEI010000064 DAVMEI010000000
VERSION     DAVMEI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.064"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(2..358)
                     /locus_tag="VYQ24_001199"
     CDS             complement(2..358)
                     /locus_tag="VYQ24_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV1968341.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(360..2183)
                     /gene="dnaK"
                     /locus_tag="VYQ24_001200"
     CDS             complement(360..2183)
                     /gene="dnaK"
                     /locus_tag="VYQ24_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1968342.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAEAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2663..3187)
                     /gene="grpE"
                     /locus_tag="VYQ24_001201"
     CDS             complement(2663..3187)
                     /gene="grpE"
                     /locus_tag="VYQ24_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1968343.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(3214..4248)
                     /gene="hrcA"
                     /locus_tag="VYQ24_001202"
     CDS             complement(3214..4248)
                     /gene="hrcA"
                     /locus_tag="VYQ24_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1968344.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4412..4921)
                     /locus_tag="VYQ24_001203"
     CDS             complement(4412..4921)
                     /locus_tag="VYQ24_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1968345.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            5529..7862
                     /locus_tag="VYQ24_001204"
     CDS             5529..7862
                     /locus_tag="VYQ24_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1968346.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMNTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTIAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVSEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            7875..9338
                     /locus_tag="VYQ24_001205"
     CDS             7875..9338
                     /locus_tag="VYQ24_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1968347.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            9338..>10012
                     /locus_tag="VYQ24_001206"
     CDS             9338..>10012
                     /locus_tag="VYQ24_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1968348.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDY
                     NFNLLLI"
BASE COUNT         3128 a         1859 c         1930 g         3095 t
ORIGIN      
        1 cttagtattt gcctatataa ttccaattga tcataagaaa tacaactact gtccaaatca
       61 taccaattat gaacggtatt acaaatttat atgtttgtat ttctaatgat atcatagctg
      121 ataccatcgc tgatacaaat gcagacaaca atactgaagt aacaaaattc cttaccgctt
      181 catcattttt gacacaacta tcatgctcag ctaatataat ttctctatat atagtcaaac
      241 attctactgg aatattatct ataaaacctt ttgctgcttc atagttgtca gcttgattac
      301 aaaaatcatt ataaactttt cgtgtatttt ttagatactc atcatccaat acactcatct
      361 tatttttccg taaactctcc gtctacgacg tcatcgcctg cgtttcctgt tgcttgtgcg
      421 ccttctgctc cttcttgagc ttgttgcgct gcttcggctt gttcgtagag tttcacagca
      481 aggccttgag ctttttcgtt caacgcttca agtttcgctt tcatgtcgtc caagttgttg
      541 tcttcttgag ctttcttaag gtcatcaagg gcagcttggg cagcgtcacg ttctgcgtcg
      601 aagcctttac cttcagtttc cttgattgtc ttttcagtcg caaagattgc ttggtctact
      661 tcattacgaa ggtctacttc ttctttacgt ttcttatcgg cttcagcgtt tgcttctgca
      721 tctttcatca tgcggtcgat ttcttcgtca gtcaaacctg agttcgattg gatgacaata
      781 gtttgttctt tttgagttcc aaggtctttg gccttaacag acacgatacc gttcttgtcg
      841 atgtcaaatg ttacttcgat ttgaggaatt ccacgaggtg cagctgggat atcagtcaat
      901 tggaagcgtc caagagtctt gttatctgct gccattgggc gttcaccttg aagaacgtgg
      961 atatcaacgg ctggttggtt gtctgctgct gttgagaaga cttgtgattt agatgttggg
     1021 attgtagtgt tgcgatcgat aagttttgta aatactccac ccattgtttc gataccaagt
     1081 gacaatggcg ttacatcaag aaggacaacg tccttgacat caccagtaat cacaccacct
     1141 tggatagccg cacccatagc aactacttca tcagggttta ctgatttgtt tggttcttta
     1201 ccagtttcag ctttaacagc ttcaacaacg gcagggatac gagttgaacc accaacaagg
     1261 ataacttcgt cgatttctga caagctcaaa cctgcatctg aaagggcttg acgaactgga
     1321 acttttgtac gttcaacaag gtcacgagtc aaatcgtcaa atttcgcacg agtcaaagtc
     1381 atttccaagt gaagaggtcc agcctcacct gcagtgataa atggcaagct gatttgtgtt
     1441 gaagttacac cagaaaggtc tttcttcgct ttttcagccg catctttcaa acgttgcatt
     1501 gccatcttgt cagtagacaa gtcgataccg ttttctttct tgaattctgc taccaagtgg
     1561 tcaatgattt tttggtcaaa gtcgtcacca ccaagtttgt tgtcccctgc agttgacaat
     1621 acgtcgaaga caccgtcacc caattcaagg atagagacgt cgaatgtacc accaccaagg
     1681 tcaaatacca agattttttc ttctttatca gtcttgtcca aaccataagc aagagctgct
     1741 gcagttggtt cgttaacaat acgttctact tcaagaccag caattttacc agcgtctttt
     1801 gttgcttgac gttgagcgtc gttgaagtaa gccggaactg tgataacagc tttggttact
     1861 ttctcaccaa ggtagtcttc agcgtagcct ttcaagtatt gaaggatcat agctgagatt
     1921 tcttgtggag tgtattcttt tccatttgca gaaacttttt cagaagttcc catcttagat
     1981 ttgatagaga taactgtatc tgggtttgtg actgcttgac gttttgcagc atcaccaacg
     2041 atgatttctc cgtttttgaa tgagactaca gatggagttg tgcggtttcc ttctgggttt
     2101 gcgatgattt tgctttcagt tccttcaaga actgcaactg ctgagtttgt tgtacctaag
     2161 tcaataccga taattttaga catgtgtttt tctccttgtt agttaatttt ctatttttct
     2221 ttttgatact cttcttaagt ggcgacgccg tcagagcttg acttcgtcaa tctctttgac
     2281 taaactttga gcctaaggtc tcaaaatttg cgcaaatagc gccacggcga agagtatcgt
     2341 gtttggttcg cttcgctcac tattacaaag ccaaacatat tttatctttc ttcatagttt
     2401 attgtcgttt cggacaagtt ttctattatg taaattgcga cacgaagagt cgaaatcgat
     2461 tttatttcga cgacgagtta gtaaggaagc taggcaaacg ccatagcgat tgccgttttc
     2521 tgacgagttg ctttagctac cgtcagaatt gccgaatcga acacgcccta agctctgagt
     2581 aaaaaagata gattttcttg tgttcatcga acacttcgtt aatctcctat ttttactgcg
     2641 agctttttac gggctttgta tcttagttat acaccactac cattgctggg cgtaggatgc
     2701 ggtcatggag tttgtagcct ttttgaaaga cttgagcgat ggtatctact gggtgttcat
     2761 cgtctgctgg gagagtttgg atggccatat ggtagttatg gtcaaattcg ccatctgctg
     2821 cgatttcttc aattccttct tctttcaaag cgtgaatcaa gctttcttgc accatcccca
     2881 agcccttctt cacatcatct gtcaaacctt caactgcaag tgcacgctca aggttgtcaa
     2941 gagatggtaa gattgctttt gccaagtcct ggctacgata acgttgcaag ttttgacgtt
     3001 cttcattggc acggcgttgg atattttgca tttctgcatg agcgcgaaga tatttgtttt
     3061 cgaactcatc tgcacgttca tttgccaagt ccaactcaga cttttcagga gttgtttctt
     3121 cagctgtttc cacaacttcc tcttcttgaa cttcttctac ttcttcattt tttatatctt
     3181 gggccatgtt cgcctccttt aatgatttca atcttaatgt acttcgtaat gattactgct
     3241 gaggtagcgg taaaaatctg tcaacttcat ggtcaaaaca cggttgacca cattgatttg
     3301 attgattagc tgttggtaat ccagattaac tggaccgata atggctagaa ttccaactcc
     3361 ccgataagga atgaggaact tactactaat caccgctagg tcagctaaac aggactcttg
     3421 accgtctgca atacgaacat tttgcatctg atcctcacgc aacccctcac gaatctccaa
     3481 ggccaccttt tgcggttggt caaagaactg ataggctgct agattggcaa aattcaagag
     3541 attgaccttg cccgccatca caatgttttc gttgaacatt tccttaaaga tgtgttcaaa
     3601 gagatcgatg acattatccg ttgttgtaaa gtaacgctgg ataatctgcg gaatctccgt
     3661 ccgaatcttg tagtgaatat ctaaaacggt gtgaccgagg aaacgttcct gaatgatgct
     3721 cttcagtttc agcaaatcct cctgcaagaa gttccttgga atcagaaact gactagtaac
     3781 cgttcgcgac tcgtctaggg taaataccgc caaggctgta tgttgcccca aaacaacgat
     3841 atcaaaggct gtcaaacgtt gcctgctcgg ctcaacatcc agtgccacta ccgtacagcc
     3901 actcaggtct gttagcaagt tagcagcctc ttgcagaata tcctccaatt tgaagaattc
     3961 ctgatcaaag gctttgacaa tctcatatac ctcattttca gccagccggt gaaaatccag
     4021 tgagtgtttc acatagtact gaaaaccagc aacacttggc atccgaccgc ttgaagtatg
     4081 agccttctca agcaaccctt gtttttctag ttccgccatg tcattacgaa tggttgcact
     4141 gctagagtta atagactctt gcaaggcttt tgatccgaca ggttcgtgcg ttttggtaaa
     4201 gatgtcaata atcagattta aaatatcctg ctgacgctct gtaaccatct catcactcct
     4261 ctctgtctga tcaattagca ctcaatgcat ccaagtgcta acttatgatt ctattataca
     4321 cccttagaag agaatgtcaa gacaaaaact caaaaaatta gcactctttt atcaagagtg
     4381 ctaaaaatca atctgaagac ctagaacctt gctattcatc tactcggtat ttccttttta
     4441 gtctgaaata accatgaatt agataagaaa tcagaaaagc atataaaaca aaaataagaa
     4501 agaaagacat agaaagatcc tctctaaaca aactcgtaca aacaacaaga ccacctaaga
     4561 aagtagttgt acaggaacga agtctagatc gcataacttc ccatttgagc cccttactca
     4621 tatagacttg atcctctgga agtaaatcag aagacgattt acgaagaatc gaacaagaac
     4681 ctgctcctat caattcccaa cctctatctc taaaatcttg cagttcatgc ttatattttt
     4741 taagaaatct agaatcatag atacggtaga tgacatcgtc tggttgacat tggtcaaaat
     4801 agaacaaacc aaaacgactc gttctatacc tccaaccttt caaatgcatc tcatgtaaat
     4861 attcttcttc cttgtccaaa tcaacaatgg tgaaaatccg aaattctact ctgctattca
     4921 ttgtcttacc ccaaaattag aaaacatgcc tggcgttatt tattagataa ttctttccac
     4981 ttttgactca atctccaaaa aatataagaa atctgaatcg caaaaactat caataaaacc
     5041 caatctatta tgaaaatcaa aaacactttc caactgaaag aactacctcc agtgacaaac
     5101 tttgagcaaa acggtagtag agctaaaaag agaagtaaaa taggaagcat ccgcattgtt
     5161 aaaatccgtt tggcataaaa aaatctttat ttaaactaaa atattatagc aaaatttacg
     5221 ccagtttttg aacggctgat gtagatattt tatactttca aaatgtttaa atgtgattat
     5281 ttatttttga aaaatagctc accagcccga ctgaaagtgc ttatagaatg ataataagtc
     5341 gcctgccgaa aacagcggaa aatagcggtg ttatgcggag ataatctgac gcgatgcgaa
     5401 agtatattgc atacttattt tcaacaattt agcagagtat ttttataagt gtgatataat
     5461 agaagtataa tttgttctga tagtttattt tatggagaag tagattttta gaatgcggag
     5521 ggttcaatat ggttgagttt ataaagtcta agaaagaaat gagtgaggag gatattaaag
     5581 caaatttcat cactcctgct attgtatcca aaggatggaa aaatggtgag catatcgctt
     5641 acgaagaata cttcactgat ggtcgaattg aggttagagg agataaggct cgtcgtaaag
     5701 aaggaaaaaa atcagactat tcactgtatt accaatttgg aactcgaatt gcaattgttg
     5761 aggcaaagga taataaacac agcgttcgag caggattaca acaagctatt gaatatggag
     5821 agattttaga tgttccattt gtttattctt cgaatggtga tggctttatt gaacacgacc
     5881 gtatcacgag agaagaacgt gagctggagt tagacgaatt ccctactcgt gaagaattat
     5941 tttctcgtat gacgaaggaa aaaggattga cgtacgaaat tacagaagct atctcaactc
     6001 catactatac agacgccttc tcaatgaaca cgccacgcta ttatcagcaa atagctatca
     6061 accgtactat tgaaacagtt gccagaggac aaaaacgagt aatgtttgtg atggcaacag
     6121 gaacggggaa aacgttcatg gcttttcaaa ttattcatcg ccttcgaaaa gctggtttgg
     6181 ctaaacgagt tttattctta gcagatagaa acatcttagt agaccaaacg atagctgaag
     6241 actttaagcc attcgaaaag gtaatgacga aaattacacc aaaacttttg actgctcctg
     6301 aaaaattaaa ttcttttgaa atttatctag ggctttatca gcaactaact ggtgaagatg
     6361 gaactgaaac acattatcaa aaatttgaca aagacttctt tgatttaatc gtaattgatg
     6421 aagcgcaccg tggttcagct aaggaaaaca gtaactggcg taagataatt gattatttca
     6481 gttctgcgac acagattggg atgaccgcta ctcctaaaga aaccaagaat gcttccaata
     6541 cggaatactt tggtgagcca atctatactt atagtttaaa acagggaatc gaggatggtt
     6601 ttttggctcc atatcgtgtt atgagagtta atttagatgt agatgtggat ggttatcgtc
     6661 cagaaactgg aaaagttgat gctaacggac aattaataga agataggtac tacggcagga
     6721 aagattttga taaaaccatt gtcattgatg atagaacgca aagagttgcc aagtttgttt
     6781 ctgattatat gaagcaaaac aatgcacgat ttgataaaac aattgttttt tgtgttgata
     6841 ttgaccatgc cgagcgaatg cgtgctgcac ttgtaaaaga gaatctagac ttagtccaag
     6901 aagactatcg ttatgtcatg caagtaactg gtgacaacgc tgaaggaaaa gctcaactgg
     6961 ataactttat ggatgtcaat tctaattttc ccgctattgt aacaacgtct aaattattaa
     7021 cgacaggagt taatgctaaa acatgtcgtt tgattgtttt agactctaat atccaatcca
     7081 tgactgaatt taaacaaatt attggtcgtg gcacacgtct ttatcctcaa aaggggaaag
     7141 aattttttac gattattgat tttcgaaatg ttaccaattt gtttgctgac cctgattttg
     7201 atggtgatcc agtgaaggtg ctggaaacag gtgcgaaaac agtcagtggt tctacgcccg
     7261 gtttcgtaag tgaggaaggt gacccagtag aaaaatatat cgttacagac aagcaggtta
     7321 ccattcttaa ttctactgtt caagtattgg atgaaaacgg gaaactgatt accgaaagcc
     7381 tgaccgacta cactcggaag aatatcttag gtagctacgc cactttgaac gattttatca
     7441 cagtttggca tacggcggat aaaaagaagc ttatcttaga cgaactttat aaaaaaggag
     7501 tttatctaga tgctattcga gagtcggagg gaatatcaga acaagaaatc gatgattttg
     7561 atttactcct aaaacttgcc tatggtcaaa aagaattaac caaaacggaa cgtatcaata
     7621 agctcaaaca aagcggatat ttatataaat atagtgagga agcgcgtgct gttttggaaa
     7681 ttttactgaa caaatacatg gataaaggta ttggagaact cgaaagcatt gaaacattaa
     7741 aacttccaga atttcagata tatggtggaa ccttcaaaat catcaatact tattttggag
     7801 ataaaaaacg atatttacaa gcaattaaag aattggagca agagctattt acagtagctt
     7861 aatgaaagga aagtatgtca attacatcat ttgtaaaaag aattcaagat atcactcgaa
     7921 acgatgctgg tgttaatggt gatgctcaac gtattgagca aatgtcttgg ttattattct
     7981 taaaaattta tgatagccgt gaaatggttt gggaattaga agaagacgag tatgagtcaa
     8041 ttatcccaga ggaattaaaa tggcgaaatt gggctcatgc tcaaaatggg gaacgggtat
     8101 tgacaggcga tgaattactt gattttgtca ataacaagtt attcaaagag ttgaaagagc
     8161 ttgaaataac ttcaattatg cctattcgaa aaacgattgt taaatcagct tttgaagatg
     8221 cgaacaacta tatgaaaaat ggcgtcttgt tacgccaagt catcaatgtt attgatgaag
     8281 ttgatttcaa tagccctgaa gatcgtcatt cgtttaatga tatttacgaa aaaattctta
     8341 aagatattca aaatgctggg aactcaggag aattttatac gccacgtgca gcgactgatt
     8401 ttattgccga agttcttaac ccaaaacttg gagaatcaat ggcagacctt gcttgcggaa
     8461 caggaggctt cttgacttcg actctgaacc gtttaagtag tcaacgtaaa actagtgaag
     8521 ataccaaaaa atataataca gctgtttttg gtattgaaaa gaaagcattt cctcatcttt
     8581 tagcagttac aaatctgttt cttcacgaaa ttgatgaccc taaaattgtt catggaaata
     8641 ctttggagaa aaatgttcgt gaatatacgg atgatgaaaa atttgacatt attatgatga
     8701 atccaccttt tggagggtca gaattagaaa caataaaaaa taactttcca gcagaattac
     8761 ggagttctga aacagctgat ttatttatgg ctgtcattat gtatcgtttg aaagaaaatg
     8821 gtcgtgttgg agttatttta cctgatggtt ttctatttgg tgaaggtgta aaaactcgct
     8881 tgaaacaaaa actggtagat gagttcaact tgcatacgat tattaggttg cctcatagtg
     8941 tctttgcacc gtatacagga atccatacga acattctttt ctttgataaa acaaagaaaa
     9001 cagaagaaac ttggttttat cgtttagata tgccagatgg ttataaaaat ttctcgaaaa
     9061 ctaagccgat gaagtcagaa cacttcaatc ctgttcgtga ctggtgggaa aatcgtgaag
     9121 agattctgga aggtaagttc tacaaatcta aatcatttac acctagtgaa ttggctgagt
     9181 tgaattataa tttagaccag tgtggctttc caaaagagga agaggaaatc ttaaatccct
     9241 ttgagttgat tcagaattat caagcggaaa gagcaacttt aaatcataag attgataatg
     9301 tattagctga tattttgcag ttgttggagg acaaataatg acaccagaac aacttaaagc
     9361 aagtattctc caaagagcga tggaagggaa attagtgccg caaaatccca atgacgaacc
     9421 tgcaagtgaa ttattaaaga gaattaaagc tgaaaaagaa aaacttatca gtgaaggaaa
     9481 aatcaaacga gataaaaagg aaactgagat atttcgtggt gatgatggga aacattatgg
     9541 gaagtttgct gatggaagca ctcaagaaat tgatgttcct tatgatattc ctgatacttg
     9601 ggagtgggtg aggataaaat caatttattg gaattttggg caaaataagc cagagaaatc
     9661 ctttaggtat atagatacgt ctagtattga tagaaaaaag aacataatca actacaaaaa
     9721 tctacaatat ctttcacctg aacaagcgcc ttcccgtgct agaaaattag tttcgcagaa
     9781 tagtgtctta ttttcaacag ttagaccata tctaaaaaat attgctgtag ttagagaact
     9841 taaagagtat ttgatagcta gtacagcatt tattgttttg gatactttac ttaacgaaac
     9901 atatttgaaa tattatttat tatcagataa ttttatcaat cgtgttaata ataaatctac
     9961 tggaacaagt tatcctgcaa tcaatgatta taattttaat ctattattaa tt
//
LOCUS       DAVMEI010000065         9965 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVMEI010000065 DAVMEI010000000
VERSION     DAVMEI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.065"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(40..804)
                     /locus_tag="VYQ24_001207"
     CDS             complement(40..804)
                     /locus_tag="VYQ24_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968349.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(900..1303)
                     /locus_tag="VYQ24_001208"
                     /pseudo
     CDS             complement(900..1303)
                     /locus_tag="VYQ24_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1483..2115)
                     /locus_tag="VYQ24_001209"
     CDS             complement(1483..2115)
                     /locus_tag="VYQ24_001209"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1968350.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2149..2850)
                     /gene="pyrF"
                     /locus_tag="VYQ24_001210"
     CDS             complement(2149..2850)
                     /gene="pyrF"
                     /locus_tag="VYQ24_001210"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1968351.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(3083..4291)
                     /gene="divIB"
                     /locus_tag="VYQ24_001211"
     CDS             complement(3083..4291)
                     /gene="divIB"
                     /locus_tag="VYQ24_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1968352.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEDDKEKEEKEEKEE
                     PESKEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYAT
                     MKDIRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQF
                     PTKFTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIK
                     VFVSELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDLDEVLVPLSEMSKKLPYYSK
                     IKPQLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNR
                     NQTTQRSSRR"
     gene            complement(4301..5359)
                     /locus_tag="VYQ24_001212"
     CDS             complement(4301..5359)
                     /locus_tag="VYQ24_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1968353.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLLELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5363..6715)
                     /locus_tag="VYQ24_001213"
     CDS             complement(5363..6715)
                     /locus_tag="VYQ24_001213"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1968354.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLVKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(6819..7460)
                     /locus_tag="VYQ24_001214"
     CDS             complement(6819..7460)
                     /locus_tag="VYQ24_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968355.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7462..9483)
                     /locus_tag="VYQ24_001215"
                     /pseudo
     CDS             complement(7462..9483)
                     /locus_tag="VYQ24_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(9566..9865)
                     /locus_tag="VYQ24_001216"
                     /pseudo
     CDS             complement(9566..9865)
                     /locus_tag="VYQ24_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         2989 a         2204 c         1762 g         3010 t
ORIGIN      
        1 ataaaaaatc ttttttgtaa aattgaacaa attgtttcat tatacgtagt cctctgaatg
       61 tagaaaaaat gtaccataaa caaccaaaca actaacaaat aaaataaaag caagatgccc
      121 actaactaag gaaagactga tatctttctg atatcccaaa gctaatgttg tcacaggttc
      181 taagtaagat agccctaaaa tagcccaaaa aataccacca accatcatat aggcaactgg
      241 gatgaaaata gctcctattt ttttcttcac tagcaaagca ctagctagtc caaaaataga
      301 gaacacagcg ccccaaactc cataccagag agtcgttaca agactataga gaaactgatt
      361 ggaatcaaat aattctttta aggcaccact ataatctcca atataaattt cctgataagg
      421 agtcactaaa agattaattc ctaataataa taaatagggg agaaaaaaga ctagaaaaga
      481 agaaataatt acagtactac ccacaatagc cagatacttc tttttagaaa ttcgacacaa
      541 ttgtgctgtt agaaaatgac tctcagcatc ctctattatc tgactagaat agggcagtgt
      601 acagataagt gcagctacta ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa
      661 aaaaatcgat aaaccttcaa ttttataaat accattgaaa gcaaggaaat ttcctaaact
      721 catgcaaaga agggtcaaaa ataaagacat ataaaatcga ggtgattgaa cgactcgtac
      781 aagattacaa atgaaaaatt ccatccttac tcctccttat aataaaaata gggtgtagca
      841 ttcttttttc atgctacacc cacaatcaac catctctaag gcttactctg acaagtaagt
      901 taataagaat ctggattcca agaacctgaa gtatgaattc ttacatgatt tccaaattgt
      961 ggcgccatag ctaacctagt accagaacca atataattgt caccacctcc attatagtac
     1021 atgacaatcc tagagccaga ccccaatgaa tataccgggg taatatctga cccactatag
     1081 gcgctacgaa tagaggtatt taacctttta ccgccaccag tgctgtcatt attattaatt
     1141 ccagcagagg cgttttcttt ctcaagctca ctagttgtat ccgaacccca acgcggcaac
     1201 gttaaatcat aatctccaat ctgtctagct aaaactgtag ggagaaacaa actagatgca
     1261 attgtaacta cgcaaaaaag gaatatattt ttcttctttt catgacatcg tcctcttaag
     1321 aagtgataaa caagatagtt gattgatcaa tttgatacat attacatttt cattatatca
     1381 ataaaagaaa gcgttgtcaa ttattttatt taaaatattg tcatatttta aataaaatgg
     1441 taaaagagac tcggtagcct cgtctctctt tatcttactg acctaacctt cttgccaatt
     1501 ttcttggtct tctttaaagc gttttagaag atcaaggccc tctggcgtga tgtaaccttc
     1561 ttcttgggct agatggataa gctcgctata gtttgaaagc gtcacaagtt taacaccagc
     1621 atctgcaaag ttcttatctg cttttggcaa ttggtagctg aaaatcgcta caactccaag
     1681 tacatctgct ccttctcgct tggctgctgc tacagcttca agaactgaac cacccgttga
     1741 aataaggtct tcaaccacta ccattttttg accttgagct acgcgacctt cgatttgatt
     1801 accagctccg tggtcttttg gtttgctacg gatgtaggca aaaggcaagt ccatcttatc
     1861 agcaataatg gctccgtgtg gaatccctgc tgttgcagtt cctgcaatca cttctacttc
     1921 aggaaaggct tctttgatag cttccacaaa accattttca attagggtac gagtttctgg
     1981 ataggctagt gtcacacgat tatcagtgta aatcggtgac ttgataccag atgcccaagt
     2041 gaagggttcc tctggtttga ggtaaacggc ttggattttc aagaggtggc tagcgatatc
     2101 tttagcaagt gtcatggtat tctcctttta tttttctaat ctagttcttt aattccagtc
     2161 ctgtgtccat tcatccttga tggcatgata agctgcaaca ggatcctcag cttgggtaat
     2221 gggacgtccc actacgatat agtcactgcc gatttgatag gcatcagcag gtgtcatgac
     2281 tcgtttttga tctccaactg caacaccagc tggacgaatc cctggtgtca gacagataaa
     2341 atctggattg gtagcctgct tgatgacttg tacttcctga gccgagcaaa caacaccatc
     2401 caagccagct tcagctgtct tcttggcata gtgaatcaca gactcttgca gactggtttg
     2461 gatattttga aactcctgca tctgggcttc tgacgttgat gtcagctgcg ttacagcgat
     2521 caatttagct tgactcccaa ggccttcacg cgccgccttc atcatctcta caccaccagc
     2581 cgcatggaca ttagtcatat cgacaccaag ctgagacaag accttcatgg ctgacttgac
     2641 tgtattagga atgtcatgaa gtttgagatc caaaaagaca ctatgaccca aaccttttaa
     2701 gtaggacaca atctcaggcc ccgctgcgta ataaagctcc atccctacct tgagataaag
     2761 gctttcttct gctgggaaaa gagctaaaaa ttccttgacc gcctcaaaac taggaaaatc
     2821 aagagcaatg actggacgat gttctcgcat aggtttctcc ttttaccttt actagtgaga
     2881 gagtctggcc aacagaagcc acgaaaaaag gaacctgcca acagcaaggt tccacgaaaa
     2941 atgggtagtc tccacccttt caccgtctaa ccttagctgc ctctctggac tgctttaaag
     3001 gttttttact attctattat ttctactagc acttgtcaag taaaaacatg gtcactaaag
     3061 aactataaga gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt tggtttcgat
     3121 tgctctcttc ctcttgtttt ttctgttctt cttcttgttt tttctcagct tccttagcct
     3181 cttgtttggc tttttcctca gcttccataa ttaatttatc cgccacagtg taactgtaaa
     3241 ttccagcttc catgtcaatc acactcggtt ctgacaattg tggcttaatc ttactataat
     3301 aaggcagttt cttgctcatt tcagaaagcg gaaccaagac ttcgtccaaa tcattcatag
     3361 tcaatcgaat taaatcggat gtcaccttgc ttggggctaa ttccaccttt tggatagccg
     3421 ccttgagttc tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt tgttcactat
     3481 cattaaagag aactgataaa taagtttctg gtaaactgtt caggctcaca gaactagtct
     3541 caagctgacc actggaaaga ataggataat gattttcacc agaaatatag taggccacaa
     3601 tatcatattc cttgacctta atagtgaact tagttggaaa ttgatagaca agttgagctg
     3661 attcaaccca atagttagac ttaatctgct tttcatattt tgccttgtct agcagaaggt
     3721 taatcgtata atccgaatcc tgaatgcctg aagcctgtcg aatatcatca gctgtagttt
     3781 gcaccgttcc ctcaacacga atatctttca tggtcgcata aggactgagc aagtaggcag
     3841 agacaatcaa taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag atatggatac
     3901 caggaatctt tgctttggct ggtttttcct ttgtagcctt tttagcaagc tttttatcct
     3961 gttcctcctt ctctttagac tctggttctt ctttctcttc tttctcttct ttctctttgt
     4021 catcctctga ggatgctact ttttcttcag actcttcctt agctgattct gaatcttcct
     4081 ggtctgtttc actctcctgg tcctgtttgt cctctgactt ctcagattct tctcccatcc
     4141 gagcttgtct ttccttttcc ttctcctcag ctagagccgc ttcttcttca gccttctttt
     4201 ttagatattc ttggtttcgt ttctgccatt ctgataactc tttcaattct tcgaggattt
     4261 ctttgtcctc atttttctta tcttttgaca tttactttcc ttatgataaa tcttttttca
     4321 acaattgata aaaatctgct agagatttca attccttaga agctttcatc ttagcttggt
     4381 aatcttcctt gtgacttagt aagtgagaaa gcttctcttc caaactatcc aaggtcaaat
     4441 cgctttcttg aaggtcttct gcatagcctt tttttacaaa gtaagctgca ttttcaatct
     4501 ggtcaccacg actagcttca cgaccaagcg gcacaatgac atgcaatttt gctatcgcca
     4561 agagctcaaa aatcgtattg gcaccacctc gtgtcacaac aatatcagcc aattccaaca
     4621 agggttgata gagatcggtc acatagtcaa cacgaaaaag attttggctc aactcattca
     4681 ggctagaatc tccagttaga ttgataatat tgtagcgctc tgttagttct ttcttatggt
     4741 ctgtcaccaa ttggttaaag acacgagcac ctgcagaacc gccaacaaac aatacagtcg
     4801 gcaatttatg attaaagtgg gtttgaatat ccaccaattc atctggttct ggattttttt
     4861 gatctgaaac cttggtcact gctcccacat gctcaacctt agacaaactc gaagcttgct
     4921 caaaggttga atacatctta gtcgcaaatt tataggcgat tttattggcc aagcccatag
     4981 acaggtcaga ttcgtgaata aagacaggca ctcctgacac acgcgctgcg ataacaggcg
     5041 gtactgagac aaagcccccc tttgaaaaaa gggtctgtgg acgaagtcgc aacatgataa
     5101 agagcgattg gacgattccc cagccaactt tgaagacgtc cagcatattt tgccaagaga
     5161 aatagcgacg caatttccca gtcgcaatgg agtggaaagt gacatccaaa cctgacttaa
     5221 ggatttcttg gtgttcgata ccacgcttgt ccccgatata gtggacttcc caaccatctt
     5281 cgatgaactt gggcattaac aaaagattga gggtaacgtg tccaaccgtc cccccacctg
     5341 taaagacaat ttttttcata ttttattctt ttaactccgc tactgtgtcg ataaagaggt
     5401 cgccacgtac ttcaaagtta gcatacatat cccagctggc attggcagga ctaagaagaa
     5461 ccacatctcc ttgagtcgca agctcatagg ccttgcgggt cgcatctgca atatctgtcg
     5521 cctccacata agcgacacca gccttgtctg ctgcccgttt gacacgttct gcagattgac
     5581 ccaggatgac catcttcttg agtccagtaa tgtctggcac caattcgtca aactcattgc
     5641 cacggtccaa accacctgca atcaagacga ccttgctgtt gtcaaatcct gataaggctt
     5701 tttgagtagc caagatatta gttgatttac tgtcgttata gaatttaaca cccttgatgt
     5761 catccacaaa ctggagacgg tgtttgacac caccgaaggc tgaaagagtt tccttgatgg
     5821 tttgattgtc cacatcacga agcttggcta cagcaatagt cgcaagggca ttttccacat
     5881 tgtggctacc tggaacaccg atttcattcg ctgccatgac tacttcacca cggaagtaga
     5941 gttgaccatc ttccagataa gctccatcaa ccttttcaag tgttgaaaat ggtacaacag
     6001 tggcttctgt cttggaagcc aagtctttta ccaagtcttg attaaagttc aagacaagga
     6061 aatcagctgc tgtcatcttg ttctggatat tccacttggc tgctacatat tcctcaaata
     6121 atccatgata atcgatatga gttggcatga ggttggtaat aaccgcaatc tctggatgga
     6181 attcttgaac acccatgagt tggaaagaag aaagttccat aacaagcgtg tccttatctg
     6241 atgctatttg agcaacctga ctggcaggat agccgatatt ccctgataaa agaccatgtt
     6301 gcccagcagc agtcaaaact tccccaatca tagtcgttgt ggttgtctta ccgttcgatc
     6361 ctgtgatacc aataatcggt gcttctgaaa tcaaataagc caattccacc tcagtcaaga
     6421 ctggaattcc cttggccaaa gccttttcaa tcatgggatt gctgtagggg atacctggat
     6481 tttttaccat gagggcaaac tcttcatcca agagttccaa aggatggcca cctgtaatga
     6541 ccttgatccc ttcttccagc aaacattggg cagctggatt gtcctcgaat ggtttcccat
     6601 catttactgt cacaatggca cctaacttgt ccaacaaacg agccgcagat tcaccagact
     6661 tggccaaacc taaaacaaga actttcttat ttttaaattg atctattact ttcatgtctc
     6721 gaactccatt tctactccta ctattttacc atttttatgg aaataaaaaa gccacaaagt
     6781 gtgtttgtga ctctttcttc taactgaatc ttaccatatc atctatgtga taaatcggta
     6841 actcgaatga cctgatccac ttgctcccaa atcagaggat tgtgggtcgc aataataatg
     6901 gtccgattcg gattttttaa agattctagg atggaaagta attcctcaga atttttgggg
     6961 tctaaggaag cggttggttc atctgcaaga atcaaaggcg gatcctttaa aattatcttc
     7021 gctagtgcaa cacgttgtgc ttctcctcct gataactcaa atataggttg cttcaaatcc
     7081 aaataagaga ggtttacaca gtttagagct tgtttcatca aagagatttt ctctttttcc
     7141 ttcaactttt taccaactaa acccagattg agattctctt tgacggtttg gctttcaatt
     7201 aagccaaaat cttgaaataa gtatcctaag taatctctaa agaaaacaga aggcttgatg
     7261 tccttaagag aagtgccatc atagatgatt tgccctttgt catatggctc caatcgtcca
     7321 atcatattca agagtgttgt cttaccacag ccacttgtac cgattaaggc ataaattttc
     7381 ccaccttcaa aatgaagatt cgtatctgaa aatagctgac ggcttccaaa ttttttagat
     7441 atattcttta gttcaatcat cctattttcc tttcataatt gtcatagaaa cacgagattc
     7501 tttctgcgct tgacggtaaa gcgtcaaaac tgcactagct agaaagacca ataaagtgag
     7561 caagccaatc accaagtctc gactgcttaa aataaagaga ctagcaccaa atacaaaact
     7621 ggcaaattgg ctaaccatat actgagcatg tgtttcaaaa aatcgtaaac ctgaaattcg
     7681 tttaatcaag atatctcggc ggaattgctc gaaatataga agattgacag aataaaatag
     7741 taataaggaa ctagctatcc ccacaatagc tcctaagatt aaagttgctg tttcagtttg
     7801 aacttcatta taacgagtta gataaacact tcttccttct ttaagatagg atacttgctc
     7861 ataaattcca gctttcttca agagttctag cccactctca tatcctttga taaagagttg
     7921 ttttccagca ttgatagacc aactagataa ggatataaaa ctatcacctg tagaagtcgg
     7981 cgtgaatacc actaaaatcg gatcagtcaa atactgagta gatacgggat tctcaccgtt
     8041 attataaaca aaccgctttt ctcccattga aagataacta acgtgcgctt tcatctcata
     8101 atccaaagga gcacttgcct cctcaccaga ttttccataa taactcaatc tttcttcaaa
     8161 aactttctta agttctgctt ctcgagagcg caaatgttct gggagcaaga ggataaactc
     8221 acctttttgg agatgggcta acttctgttt ggtctcagca tctaccacga ccttttcctt
     8281 gtccaaataa ctgggactaa catagagcgt attagcatct ggactatagg tatccagtgt
     8341 ctctccctgt tcattttttc cttgtggatt ggcaaaatgg agcagattat cctttacata
     8401 aagagcttgt tcctcttcga ttgcttcctt ggcaaaggca taccacttgc tctgattttc
     8461 tgtatctttt cctctatcac ctaagccaaa agaaatttga taatagtctg ctctatcctg
     8521 ccatgcttgt tttgaaattt caagttcttt caatcgttgg taagacgtca aacctgtctt
     8581 aacagcgtat cctactgtaa agacagcaac caactgacac aatagggtta aaatcatcag
     8641 gcgtttaagg ggtaatctcc ccttaataac gggaactaat gctttgtaac tcaaactcat
     8701 tagataaaga agcattagta aaattgaaat cgccaataaa aacaacagat agaaactaat
     8761 cccaaaacca taggtggcta acaagatagg ataaaacaaa ccttgactaa aaagaacgac
     8821 tcccccacct aggaaggaaa ggagggctga tagaaggagc tatttgatat cagtagataa
     8881 agaatgcccc atgatggata agagagtctg accagaaaag agttttatac ctgctgctct
     8941 catttcctta atccgagtga taatcactaa agcaaagaaa gataagccaa atattgctaa
     9001 actaattaaa ataaggggat ttagtaatat tcgaaaagca agaaaatagg gcggtatctt
     9061 tcggtcagca cttgctttat aacccaaatc tcctaattta tcggcaagct tttctttcgt
     9121 caaggagcct gacaaaagga gataactatt tagcggatca ctacgttcac gactttcttg
     9181 gctagcttct tggaattctt ttggtaaagt tccctgacca taagttgcat aagtaaagtg
     9241 agtcgtccca tccttactcg gctctacaat tcttctagct attaaactct gttctgagtt
     9301 tgcaaaattc tccaattcct gttcaaatac ctcacgcgtc ggttcctgag tatctttttt
     9361 gacacgaagt aaagaaacgg aatcatagct tgcatataaa tattgtggcg cacgtaagac
     9421 aataatccaa gcaaggaaga agctgagaaa aaaagttgat aataatatga atagtttctt
     9481 catagtagac tccttggtag actccttgta aacaaaattc cccctgtaat ttcttacaag
     9541 gggaacgatt taaatcaatg aacgattagt cataatcaca gtaaaatgct acttgttctc
     9601 cccatttagt ccaaatccat gcataggagg tttttccagc aggagcttct cctttatcag
     9661 aatgaccatc ccatctgctc acgatggatg aataatgata ttttttacca tgatagtaat
     9721 ttgaaaaagc ctaaccacct cctgaacctt ctccatatgt ccatactcct ccatctggat
     9781 attatacagc agctgatgca gctcccaata atgtaaaact tgaaataaga gctagagcaa
     9841 gtaatctatg ttttttcgtt ttcattttat tttttctttc aaaaaaatca caccttgagc
     9901 aacaatgcaa caaaataaat cctcctctct cttttattga aaccgctttc ttatgtgata
     9961 agaat
//
LOCUS       DAVMEI010000066         9921 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVMEI010000066 DAVMEI010000000
VERSION     DAVMEI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9921
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.066"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            63..311
                     /locus_tag="VYQ24_001217"
     CDS             63..311
                     /locus_tag="VYQ24_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968356.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            442..1614
                     /locus_tag="VYQ24_001218"
     CDS             442..1614
                     /locus_tag="VYQ24_001218"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1968357.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1614..2888
                     /locus_tag="VYQ24_001219"
     CDS             1614..2888
                     /locus_tag="VYQ24_001219"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1968358.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQIANPKLAQEKIASKKAELLELANQAYPSIVKRGGGASDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(2923..3888)
                     /locus_tag="VYQ24_001220"
     CDS             complement(2923..3888)
                     /locus_tag="VYQ24_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1968359.1"
                     /translation="MVTKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(3869..5323)
                     /locus_tag="VYQ24_001221"
     CDS             complement(3869..5323)
                     /locus_tag="VYQ24_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1968360.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGIYYELDYGHKTN"
     gene            5700..7583
                     /locus_tag="VYQ24_001222"
     CDS             5700..7583
                     /locus_tag="VYQ24_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1968361.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     SQGNKVKAGDVLGKFDSNKIAAAGLDDTTMVIVTNTGDYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7732..8619
                     /locus_tag="VYQ24_001223"
     CDS             7732..8619
                     /locus_tag="VYQ24_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1968362.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVVRHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8939..9556)
                     /locus_tag="VYQ24_001224"
     CDS             complement(8939..9556)
                     /locus_tag="VYQ24_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1968363.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
BASE COUNT         2866 a         1975 c         2229 g         2851 t
ORIGIN      
        1 caggaagtct actactttcc ttattgtctg taaatctact gaccttgttg tttatcccag
       61 tcatggtttc tagttcgggc tcagagtttc aaagtggatg gcaagagcat caattgattg
      121 ctgagaaggt tagtaaaaca cttgataaga catttgataa ggatgtcaga gaaattccga
      181 ccagtcagtt ttatcaaaaa tttgtagatg agatgggaag gacttactca ggaaatttga
      241 tcctccagga gctgataact gtgaatggag cttataaagc tacttatatc ggtgaactct
      301 ctagcaacta atcattcttt aaaatgatga ccaataagag gcttgatttg gtgaatcaag
      361 tcttttttct ctcttttact gccatttgtg ataaaatagt atagaacgat tttttacatg
      421 aatgataaaa cagaggtaaa tatgacaatc ggtattgata agattggttt tgcgaccagt
      481 caatatgtct tgaaattaca agacttagca gaagcgaggg gaattgaccc tgaaaaatta
      541 agtaaaggac tcttactcaa ggaattgagt attgcgcccc taactgagga cattgtgacc
      601 ttggcggcca gtgctagtga ctctatttta actgagcaag aaagacaaga agttgacatg
      661 gtcattgtgg ctaccgagtc aggaattgac cagagtaagg ctgcggccgt ctttgtgcat
      721 ggcttgctgg gcatccagcc ctttgctcgt agtttcgaga ttaaagaagc ctgttatgga
      781 gcgactgctg cccttcatta tgccaaattg catgtggaaa attctccgga gtccaaggtc
      841 ttggtcattg ccagtgatat tgccaaatac ggtattgaaa ctccaggaga accaactcaa
      901 ggtgctggaa gtgtagctat gttgattaca caaaatccac gcatgatggc ctttaataat
      961 gacaatgtag ctcagacccg tgacatcatg gatttctggc gaccaaatta ctcgacaact
     1021 ccttatgtaa atggtgttta ttctacccaa caatacttgg atagtttgaa aacgacttgg
     1081 cttgaatatc aaaaacgcta ccagcttact ctggatgatt ttgcggctgt ttgtttccac
     1141 ttgccttatc ctaaattagc gctaaaaggc ttgaaaaaaa tcatggataa gagcctgcct
     1201 caagagaaaa aagacctctt acaaaagcat tttgaccagt ctattctcta cagtcaaaag
     1261 gtggggaata tctacacagg ttcacttttc cttggacttt tgtctctctt ggaaaataca
     1321 gatagcttga aagctgggga taaaatcgcc ctttatagtt acggaagtgg agctgtggct
     1381 gagttcttca gtggtgaatt ggttgaagga tatgaagctt atttggataa agaccgcttg
     1441 aacaagctca accaacgaac tgccctatcc gttgcagact atgaaaaggt cttttttgag
     1501 gaagtaaact tggatgaaac aaactctgcc cagtttgctg gctatgaaaa tcaagatttt
     1561 gccttggttg aaattcttga ccaccaacgc cgttatagca aggttgaaaa ataatgaaga
     1621 taagttggaa tggattttct aaaaaatcat accaagagcg cctcgagctg ctaaaagctc
     1681 aggcgctcct tagtcctgag agacaagcta gtctggagaa ggatgaacag atgagtgtga
     1741 ctgtggcaga ccagctgagt gagaatgtgg tgggaacttt ttctctgcct tattcgctgg
     1801 ttccggaggt acttgtcaac ggtcaggaat acaccgttcc ctatgtgaca gaagaaccct
     1861 ctgtggttgc ggcggccagc tatgccagca aaatcatcaa gcgtgcaggt ggttttactg
     1921 cacaagtcca tcagcgccag atgattgggc aggtagccct ttatcaaatt gctaatccta
     1981 aactagcgca agagaagatt gccagcaaga aagcggagct cttggagctt gccaatcaag
     2041 cctatccttc tatcgttaaa cgtgggggtg gggcgagtga cctgcatgtc gagcagataa
     2101 aaggtgaacc agactttctc gttgtttata ttcatgttga tacccaggaa gccatgggtg
     2161 ccaatatgct caacaccatg ctggaagcct tgaaaccagt cttagaagaa ctcagtcagg
     2221 gacagagtct catgggaatc ctgtccaact acgcgaccga ttctctggtg actgcaagct
     2281 gtcgcatggc ctttcgctac ttgagccgcc aaaaggatca aggacgagag attgcggaga
     2341 aaattgcatt ggctagtcag tttgcgcagg ctgatcctta ccgagctgct actcataata
     2401 aaggaatttt taatggtatt gatgcgattt tgattgccac tggtaatgac tggcgtgcca
     2461 tcgaagctgg ggcccatgcc tttgccagtc gagatggacg ctatcaaggt cttagctgct
     2521 ggacgctgga ccttgaaaga gaagaattgg tcggtgagat gaccctgccc atgcctgtag
     2581 cgactaaggg tggctctatc ggcctcaacc cacgtgtagc tctcagtcat gatctactag
     2641 gaaatccttc tgccagagaa ttagcccaga ttatcgtgtc catcggtctt gctcaaaatt
     2701 ttgcagccct caaagccttg gtaagtacgg gcatccagca aggtcacatg aaactacagg
     2761 ccaaatccct agctctccta gctggggcta gtgaatctga agttgctccc ctagtagagc
     2821 gcctcatctc agataaaacc tttaacctag agacagccca gcgctatctc gaaaatttaa
     2881 gatcataaaa actcagacga atcggtctga gttttcttgt gtttaaatac tttttcctgg
     2941 taatagtgta actggtaaga agtaaccagt tgtggcgact tccttgcctt caatcttttg
     3001 caagagaaga tcaatagtga gacaagcaat ctcttccaaa ggttgcttga tagtagccaa
     3061 ttgagggtag taattttcga taaagtaggt cccatcatag ccgatgacct tgagctcttt
     3121 tgggacagaa atgcccaatt cttgagcgat tttaatgacc agaatagctg tcaaatcatc
     3181 cgaagcaaaa atggcatctg gtttttcccg ggtcaagata ttcttgattt ccatttcttt
     3241 tctgacggga gaaaagtcac tggaaacatt gataatagga gcttttggga gtacggatgc
     3301 aaaaccagcg tggcgcagtc cggttggcga attagaattg tcattccctg taatcatgat
     3361 gatagactgg gcacctgtct tgaccaaggt ttgggcagca agaaccccac cagcatagtt
     3421 gtcagaggag acaacaggga tgtctggcga taggtttcgg tcaaaggaaa taatcggcgc
     3481 tgtcacacga ttgtagtctt cgattcctag gttgtgacta ccagaaatga tgccgtctac
     3541 ctgattggct tccaacattt cgatgtattc gcgttccttc tcagaatcat gttcactgtt
     3601 gcagatgatg gtcttgtaac catttttgaa gagttggtgt tccaatttat caatcaattc
     3661 tgcatagaaa acattggaaa tattggggaa aatcaagccg attaacttag ctgattttcc
     3721 ttgcagacta cgagccaggt tgttgggttt atagcccaat tctctcatgg cttcattgac
     3781 tttttggatg gttttctcag atagataccc ttttttattg ataacccgag aaacggtagt
     3841 aggactgacg cctgcaagtt tggcgacatc agttagtttt gtgaccataa tctaattcat
     3901 agtaaattcc agttgggttt ccagatttga tcaggatacc attttggtcc gcatgtggga
     3961 agacacgacc agaaaatact ttttctcctt tattgatgaa aatttcaaag acagagttat
     4021 cgatgaagat tgtagcagta gtagcctgat tctcgatagg gcaagaacga gttgtcccaa
     4081 attcttgggc atactgttct ccagcctggc tacgatccac tgtcacttga ccgtttacaa
     4141 ggtcaaagtt gattgaaagt cccttacctt ctttatcagc aagtaagaca atctcgctct
     4201 ggctattagc ttccaagttg agttcaagtt cgtaagtgtt cttggtttgg gaacggtttg
     4261 agaaggcttc ttcagaagca cgaaggtcct taatagcagc gactggatac tggtagagct
     4321 tgtcgtcttt gatagtgagt tccttgacca aagagaaggt tccttggtgg tcaaaacggt
     4381 cagatgggta agaaacatct ggcaaaccaa gccagctaac tgctagagca cgcccatcag
     4441 gagcgttgaa ggcttgagtt gcataggctt cgaaaccgta atccatgttt tgaagttgag
     4501 acacatctac cattttggca tttttagggt caaaggaagc cccaatctta tacatatttg
     4561 gaaagatatt atcgtagtct agaactttct tatccaatcc ttgtggacag tagagaagga
     4621 caggatgttc ctctacaaag accagattag gacattccat catgtaggca gtacggtcgt
     4681 tagcaaagtc aaggtcgcca actgcttgcc agtttgtgta gtcgttattg acagccttgt
     4741 agagacggac gaagcctttt ttctccaagt cctgtccacc gacgacagca tagtattgac
     4801 ctttaaagtt aaaaatttgt ggatcgcgga agtggtcagt agagtctgct ggctggtcaa
     4861 tcaagatctt gtcaatcttt gtaatcttac cctccttgtc catcaaagca ccgatctggt
     4921 atgggtgacg gatccagttt tcatcgcgaa catttcctgt ataaaatagg aataagttat
     4981 cgccaaattg catggcagaa ccagagtagg caccgtggct atctaatgga gtatctggta
     5041 aaactttgat tccagtttct ttaaagtgaa ccaaatcatc actttctagc tgtgcccaag
     5101 atttcaaacc gtgggctgca ccaaaaggaa aattctggta aaagaggatc cacttgccat
     5161 caaagtaaga aaagccattt gggtcgttga gaagtcctgt ttttggctca acatggtaat
     5221 gagtatgcca tggagattgt gccatatttt cctttatatg ttgaatttct tcttgcgtcc
     5281 aatcttgata aagtctgtaa cgacgctcag ttgtccattc cattcattcc tccaaaaatc
     5341 ttatcacttt taatagtaac tgttttcgat aaaaaatgca agccttttat attaaaaaag
     5401 agaaaaaact gtcaaacgat tatcataaaa taaatacagg aaatcaatca aatttttacg
     5461 aaaatgtgaa agataatgaa ataaaagact ttttaaccaa ggtttcaaac ttatttttca
     5521 agaaattcct gatgaaacag tcaaaaacaa tcaatctaaa gcttgcccac taagtagata
     5581 gaatattttt tctgcaaaac gcttgacata tttttaaaac atgataaaat aatagtcgta
     5641 agggcgaata aaatcgcttt caaacaagaa caaaatgtta tataaggaga tttttgcaaa
     5701 tgaacaatca ggaaattgca aaaaaagtca tcgatgcctt gggcggacgt gaaaatgtca
     5761 atagtgttgc ccactgtgcg actcgtctac gtgtcatggt caaagatgaa gagaaaatca
     5821 ataaagaagt gattgagaac ttggaaaaag ttcaaggtgc tttctttaac tcagggcaat
     5881 accaaattat ctttggtaca ggtacagtta acaaaatgta cgatgaagtt gttgtacttg
     5941 gcttaccaac atcatctaag gatgacatga aagcagaagt tgctaaacaa gggaactggt
     6001 tccaacgtgc tatccgtact ttcggtgatg ttttcgttcc aatcatccca gttatcgtag
     6061 cgacaggtct cttcatgggt gtgcgtggtc ttttcaacgc tcttgaaatg ccacttccag
     6121 gtgactttgc aacttacaca caaatcttga cagatacagc cttcatcatc ttgccaggtt
     6181 tggttgtgtg gtcaaccttc cgtgtatttg gtggaaatcc tgccgttggt atcgttcttg
     6241 gtatgatgct tgtctctggc tcacttccaa acgcttgggc agttgctcaa ggtggtgaag
     6301 taacagcgat gaacttcttt ggtttcatcc ctgttgttgg tttgcaaggt tctgttcttc
     6361 cagccttcat catcggggtt gtcggagcta aatttgaaaa agctgtccgc aaggttgttc
     6421 cagatgtcat tgacctcttg gtaacaccat tcgtgacact tttggtcatg tctatccttg
     6481 gactctttgt cattggacca gttttccacg ttgttgaaaa ctacatcctt attgctacaa
     6541 aagcgattct tagcatgcca tttggtcttg gtggtttctt gattggtggg gttcaccaat
     6601 tgatcgtcgt gtcaggtgtg caccacatct tcaacttgct tgaagtgcaa ttacttgctg
     6661 ctgaccatgc taacccattc aacgctatca tcacagctgc tatgacagct caaggtgctg
     6721 ctactgttgc ggttggtgtt aaaacaaaaa atccaaaact gaaaacactt gctttcccgg
     6781 ctgctctttc tgccttccta ggtattacag agcctgctat cttcggggtg aacttgcgct
     6841 tccgtaaacc attcttcctt tcattgattg ctggtgcaat cggtggtgga ttggcttcta
     6901 tccttggact tgctggtact ggtaatggta tcaccatcat ccctggtaca atgctttatg
     6961 tgggtaacgg acaacttcca caataccttc ttatggtagc tgtatcattt gcccttggtt
     7021 ttgctcttac ttacatgttt ggttacgaag atgaagtaga cgcaactgca gctgcaaaac
     7081 aagctgaagt ggctgaagaa aaagaagaag ttgcgccagc agctcttcaa aatgaaacac
     7141 ttgtaactcc tatcgtcggt gatgttgtcg ctcttgctga tgtcaatgac ccagtcttct
     7201 caagtggagc tatgggacaa ggtatcgctg tgaaaccaag ccaaggcgtg gtctatgcac
     7261 cagctgatgc tgaagtttca attgcctttc caacagggca cgcttttggt ttgaaaacaa
     7321 gaaatggtgc tgaagttttg attcacgttg gtattgacac tgtatctatg aacggtgacg
     7381 gttttgaagc aaaagtttct caaggtaata aggtgaaagc tggcgatgtt ctgggaaaat
     7441 ttgactcaaa caaaatcgct gcagctggac ttgatgatac aacaatggtt atcgttacaa
     7501 atacaggtga ctacgcttca gtagctccag tcgcaacagg ttcagtttcg aagggggatg
     7561 ctgtgatcga agtgaaaatc taatcaatcc tctctaatgt gaaaacgaac aaatgacatg
     7621 tttgttcgtt tttattagat agtaagattt acagaggaaa atctaaaaaa tagagacatt
     7681 tagactttcg aagtatgcta taataaagaa aataaaaaca agaggtttat catgacaaaa
     7741 ttatatggaa gcttggaagc gggcggtaca aagtttgtct gtgctgtcgg tgatgaaaac
     7801 tttaacgttg tagaaaaaac acaatttcca acaacaactc caatcgaaac aatcgataaa
     7861 accattgagt tcttctcaaa attcgataac cttgctggtc ttgcagttgg ttcatttggg
     7921 ccgattgata ttgacaaaaa ctcaaaaact tatggcttta tcacgacgac tccaaaacca
     7981 aactgggcaa atgtggactt gcttggtgcc cttcgtcgcg ccctaaacgt gccaatgtac
     8041 ttcacaacag acgtaaacag ctctgcttac ggtgaagtgg ttgcccgtaa caatgctggt
     8101 ggtcgtatcg aaaacttggt ttactacaca atcggtacag gtatcggtgc aggtgtcatc
     8161 caacgtggtg agtttatcgg tggtgtgggt caccctgaaa tgggtcatta ttatgttgtt
     8221 agacacccaa tggatattga aaaagagttt aagggtgttt gtcctttcca taagggatgt
     8281 ctggaaggct atgcagctgg tccaagtttg gaagctcgta caggtgtacg tggggaaaat
     8341 attgaactca acaaccctgt ttgggatgtt caagcctact atatcgctca agctgcggtt
     8401 aatgcgacag tgactttccg cccagacgtg attgtctttg gtggaggggt catggctcaa
     8461 caacatatgc tggaccgtgt ccgtgagaaa tttacatctc ttcttaatgg ttacctacca
     8521 gtaccagatg tgcgtgacta tatcgtgacg ccagcagtcg caggaaatgg ttctgccaca
     8581 cttgggaact ttgttcttgc aaaagaagtt tcaaaataaa gcacaaaatc cgcttgggaa
     8641 accaaacgga ttttgtggtt gaaaacaata tttttgagcc tttccttaag tgattacgga
     8701 cggtagcgac tttcttcgaa attccatacc taaactttga gacactaggc tcaaagtttc
     8761 cgaatacctg aaaccattag atttcaggtg tttctatcac ggcgggaagg ctaggtaagt
     8821 cctcgcctca ctatcgaaaa atatgcatga tttccatacc aaagtatatt tagtatcaag
     8881 ctatagttaa tctacgcttt aaaaatccac ttgggaaacc aaacggattt tttacgtgtc
     8941 aaagcatttg ccagaaaaag tcaataatat aggtcagacc gtaggtataa accacgaggg
     9001 taaagggttt ggtcagaatg atgatggtca tgtagcgctt gaaactcatc ttggtcaggg
     9061 cagccagcat acagagaaag tcagctgggc taatgggcca aatcatcata aaaataaaga
     9121 agcggtcaaa acgattgccc ttatctagcc agccgatgta cttgtcgtag gtgcgcttgc
     9181 tgacgacaga ctggacaaag gcagctccgt ataggcgcac tagataaaag ataatggcac
     9241 agccaatcac gatgccgata tagttgtaga tagtcccgat gatgtgcccg tagataaaga
     9301 ccccagccac cgaggtcaag gcccctggaa tgatagggac gacagtctgt aaaatctgta
     9361 aaaagataaa gagaggtgga ccccagatgc ctgcctgctg gataaaggca gagagggttt
     9421 ccttggattg taaaatccca gcctgataag cccaaataca gaaaatcaaa ctcccaaccc
     9481 caccgacaat cgatgagata ttgataactc gctgcagaag gggagaaaca gctttcattt
     9541 gtttatcctg tgacatgtaa actcctcata gatagtatat ttctactata ccatattttg
     9601 gaggagaaag ggggaatgag tcttttttga taaggaatag aaagtaaata gcccttcgag
     9661 acttagaata ggtctgtcaa ctaaaggtta tatgcattct aggtttcgaa taatcgcaat
     9721 tccataaatt taaaagcatt ctaagtttcg aatagcttat ctgagataat tttgaggcat
     9781 tcgcgctttc gaatagtcat tttccctaaa gtttagatgt attctgaagc tagaaagact
     9841 aaaatctaaa agtagtaaga gtctctctct gatggacgta atttatggct agtatgaatc
     9901 ctttgaattg ttagttaatt t
//
LOCUS       DAVMEI010000067         9919 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVMEI010000067 DAVMEI010000000
VERSION     DAVMEI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.067"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(98..610)
                     /locus_tag="VYQ24_001225"
     CDS             complement(98..610)
                     /locus_tag="VYQ24_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1968364.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNFPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            complement(635..1615)
                     /locus_tag="VYQ24_001226"
                     /pseudo
     CDS             complement(635..1615)
                     /locus_tag="VYQ24_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            complement(2091..2900)
                     /locus_tag="VYQ24_001227"
     CDS             complement(2091..2900)
                     /locus_tag="VYQ24_001227"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1968365.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            complement(2924..3457)
                     /locus_tag="VYQ24_001228"
                     /pseudo
     CDS             complement(2924..3457)
                     /locus_tag="VYQ24_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285096.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            complement(3459..4670)
                     /locus_tag="VYQ24_001229"
     CDS             complement(3459..4670)
                     /locus_tag="VYQ24_001229"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1968366.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="VYQ24_001230"
     CDS             complement(4684..5367)
                     /gene="rpiA"
                     /locus_tag="VYQ24_001230"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1968367.1"
                     /translation="MENLKKMAGIKAAEFVKDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQIIAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRRFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            complement(5665..6504)
                     /locus_tag="VYQ24_001231"
     CDS             complement(5665..6504)
                     /locus_tag="VYQ24_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1968368.1"
                     /translation="MKVIDQALLEKVIIERSRTSHKGDYGRLLFLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEAMSFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDAFGEDLVKQVFAGLRQNQILIVDGGALTILARTSLSFSSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASALTSFPQGTILVEKGSATRIWQAGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFAGQFKQASLYERVAVATHLHSAIAQELAQEQYVVLPTEISNCLPKVMK
                     RYV"
     gene            complement(6518..7339)
                     /locus_tag="VYQ24_001232"
     CDS             complement(6518..7339)
                     /locus_tag="VYQ24_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEV1968369.1"
                     /translation="MILKIYNREYSLQWDGIYYLALIDYPNIQEWELEKIAKFIAYEK
                     LHKRQTSIECADSCLKKEILDYICQHPFLPPFTPTDKRVASTYDLHKRLVTSDYCSHT
                     TTIDAAISIFKTGQLLSAVKAFRRDAEELVLDSRNAASDPIDYFDYVMLGWSNTSSGY
                     RLAMERLLGRAPSEKELQDKFIPGVSFHFIYTDLIKVTGYIFDGYHVAKIKDMLNLFS
                     ELYICIIPTHNKSQFENIIPTKIQDRVHYLDYAGEDLEEWTKKVYQVVLKQSDKG"
     gene            complement(7476..8333)
                     /locus_tag="VYQ24_001233"
     CDS             complement(7476..8333)
                     /locus_tag="VYQ24_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1968370.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIELEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            complement(8487..9221)
                     /locus_tag="VYQ24_001234"
     CDS             complement(8487..9221)
                     /locus_tag="VYQ24_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1968371.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            complement(9221..9904)
                     /locus_tag="VYQ24_001235"
     CDS             complement(9221..9904)
                     /locus_tag="VYQ24_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1968372.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRLKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
BASE COUNT         2913 a         2303 c         1711 g         2992 t
ORIGIN      
        1 aattttgttc tttcacagag agctacctga gttctattca agctcagata gtactttctt
       61 ataaactaga caaactaact gtcattgtac catcagatta caagacatca tcgtcactca
      121 ccttggaatt caatgtcgta ccccaatggg taattttacg gtggggttga gctaaaattg
      181 gtctgttttc atagattgtt tgccatctat tccatagtag gcccgtcttt ttctcaatct
      241 taactcgcag atttctcata ttttctttga ttgggaagtt gaggacaaaa cctgcagtct
      301 ggttgcaacc gtttccttcc caagaatgac tacgaacaac ttggtttcca tctttatcta
      361 ctggaacttc ttcccaagtt atggagtagc gggcaatgta agctccactg tgttgaattg
      421 tcaatgtttt atctttcaca ggagtctgac tgattggttg aactggctta gaaacttgtg
      481 tcgccgtttc agcattcgta gctataaagc ggaaaacttc aacttctgca agactgaggg
      541 cattatagcc ttctagccca atccgaacat agcgcgcttt tttatggtta atcgtaattt
      601 gtgctgacac atctttagga gatattatac gttttcactc aatttcttta ccagaactgt
      661 ctaaaagaat gacatcaaag tttgccaatc tatcctgggc agtgtctgtt cggttgtaaa
      721 tattgatttg gcgaatggtt tcttctttag ccaaatctac ttgccaccaa ggcttagatt
      781 ggaagtttgt atgagtgaca gaattgtgac catagttacc atcgactttg ccatccacag
      841 ctcttctagc atctcctccg aaagctgtcg aactttgagt aacctgtttt cctaaggcaa
      901 tattttccat tggttcagcg cttggttgac tagaagattg aggtagagcc tgtttcttaa
      961 agaatcgcac tttatccaat ttcggatcac gataaccatg tttatccaag gtagcttgtt
     1021 taccaatatt aaagttagga atatcattca ttggactttc aaaatagcca cgagaacggt
     1081 gtttaaagcg tgaattacct gtaaatgtaa caatatcacg attattatga atcaagggag
     1141 tcacaacatt gtcattatca accacataat gcttaatttt tccactaaca gctaatttac
     1201 gactttccaa accaacatct cagaaaccat tcttagcatc ccaaacagtt ctggaagtaa
     1261 tcactttagg agcactgaaa gttgtcactt tcctcaatac atggttataa aaatcaggat
     1321 atttttggta atcttcaacc gctgcaatct gagctaggta gcctccaaga gaatgacctg
     1381 tcatatacaa cttagtaata cttatatcct tggctaattc cccaacaacc ttgcggatgt
     1441 catccgcttg agctggctct gttatctcta cttttgactc ttcccttttg ttttcaagaa
     1501 cttcacttgc ttccggaagc gtttgaggag ctagtcttgg ctcctctcta agaggagttt
     1561 ctagttttgg agcatcattt tctgactctt tcaattcctc cttagaatct agcacaccta
     1621 ttaccacttc agttgttaca tcttgcttta cttcattggc tgaaattcca gctggagcta
     1681 gaaatcaaag ccgactacaa cagatacaac tccgatactt aattttttat gccaaagcgc
     1741 ataattcgtt gaccaatttt cattatttct tatctttctt ttcgtttatt tacaagcaga
     1801 ctcaagcgaa atctcattgt gctatatatg ataagactga gaaattcaga aagtcaattc
     1861 attctcatct ctccttatca tactagtctg cttggctagg aagctaatgc tagtatccta
     1921 gaatcttgtc tttcacaatg ctggctatca gatcagacac acctattctt ccgcttcttt
     1981 cagttataag agcaggaaga agctcatagt attcctctgt ttcttcttta atacggatag
     2041 gcagtcaatc ctgaaaagac agaggcactc ccccttttaa atcttttttc ttacaattca
     2101 gcaagaatcg ctttaagcaa gcctttaaaa tcacctttaa cacgttcagt cacttctaca
     2161 acttcttcgt gattgagttc ttcttggaaa ccagccgcaa agttagtgat acatgaaatt
     2221 cccagaactt tcaagccaga gtgggctgcc acgataactt caggaaccgt agacatacca
     2281 actgcatctg ctcccagtgt cttataggaa cgaatttctg ctggtgtttc ataagtcgga
     2341 ccagtaactc cgatatagac accttcatca agcttgatat taagtttttt agccacttca
     2401 tgggcagtag cacggtattc tggtgtgtag gccctagaca tatctgggaa acgtgggcca
     2461 aagtcatcca agttttcacc catcaatgga ttttgccccg tcatgttgat atggtctgag
     2521 atagccatca aggtaccagg accaaatccg ataccgccag ctgcattggt tacaataaca
     2581 ccttcacatc caagaacttt catcacacga actgggaaag tcaccacttc cagaggattc
     2641 ccttcataga aatggaaacg cccttgaaga gccaagacct tgcgacctgc cagttcacca
     2701 tataccaatt taccagcatg accgactact gttgaacggc cccagtttgg aatctcagca
     2761 tagtctacta caactggatt ttcgatttct tctgccaatt ctccaagtcc tgacccaagg
     2821 attagaccga actcaggggc tgcaattccc ttttctttca ggaaagtagc agtttcatgg
     2881 attttgttta aaaatgtcat tgtgtgtctc ctttattatt tttttagcat ttgaccaatt
     2941 ttgtcaaagg ttttagcatt acgaatagta atgtggcggt agaaaggcac ctcagcaagt
     3001 acttatgata ggcagtctta gaataggatt cttcagaaaa tttcccctag aaaatcccaa
     3061 gttttccaaa ataaagcact tcatctttca gctctaaact ttcaactgtc gcgatgactt
     3121 ggtccacatc caaaccctca gtgtaaaaga gaaaatcttt tcgtgccaaa tctctgctcc
     3181 accaagctgg tagattttca agttccgcct caaagtcctc tagactcagt aaagaaaagc
     3241 tctgaataaa tggataatgg actgcaaaga aagtctctag cttttcaacc aattgggctt
     3301 tggaatctat cgaagtaaag aaaatattgc cactgttgat gtagctctca accttttcca
     3361 gtcccaagtt tgtcaattct tgacgaagct ccgccatgac gaccttattc ttaccaccaa
     3421 cattgatacc tctcaccagc aaagcatagc gcgtcatctt ataccaattt atctaagaaa
     3481 ctttccccaa tcatagcagt ttccacacca aagttatcgg caacagtcgc tgaaatatct
     3541 gcaaaatgtc ctactggaat gagaccattt cctttaaagg cagggctata ggccaacaat
     3601 ggaatatatt cccgagtgtg atccgttcct gcatacgttg ggtcatttcc atggtccgca
     3661 gtaatcaaga gaaggtcatt ctctctcata gctgcgataa tttcaggtaa gcgttcatca
     3721 aactcatgca agcaatcacg gtaaccgtga gcattacgac gatggccgta aagggcatca
     3781 aagtcaacta ggtttgtgaa tgagaatcct ttttcaaact cagcaagtcc catagtcttc
     3841 aatagtgtat caattccatg actatttgac ttgttgtgac ccatgtcatg gttgatacca
     3901 gcaccgttaa agatatcgtt gattttaccc acagcataag tatcgatacc agcctcattc
     3961 aatttatcca aaacagttgg ggaaaatgga gatacagcca agtcacgacg gtttgccgta
     4021 cgagtgaagt tacctggttc acctacataa gggcgagcaa tgatgcgacc aagaagggca
     4081 ggacgctcaa gggtaatcga acgagcgtat tcacagatac ggtacaattc atccaaagga
     4141 ataatgtctt cgtgggcagc aatctgcaaa acagggtcag ctgaagtata gataatcaac
     4201 tctccagttt ccatctgacg tggtccaaaa tcatcgataa cagccgttcc tgaataaggt
     4261 ttgttggctt cacgaataac cttgcgtcct gagaattctt cgatttttgt caggatttct
     4321 tctgggaatc cgttccagaa agtatcgaaa ggctcagtaa tgttgagtcc catgatttcc
     4381 cagtgtccag tcatagtatc cttaccaaga gatacttcct ctaattttgt tgcatatcca
     4441 gttggattgc tttcagctgc tacagtctta agaggagttt cacgaggaat atttccaaga
     4501 cctattttag ccatgtttgg gacattcaaa ccaactgttt ttgaaatgtg tcccagtgtg
     4561 tcagaagctc catctggaac ccctgcattg acaaagttat tagcatctgg tgctgcaccg
     4621 attcctacag aatccagtac caccaaatga atacgattaa atttagacat cttatgcccc
     4681 cttctatttt cctttttttg aagttgaaat ctgaactcca tcccgtccag caacgatgac
     4741 cttatccacc atttggttga ataaaccatg ctccacaaca ccaacgacat ggtccaattc
     4801 ttgtccaaaa gcaattggat tttcaatgac atccaaggcg aggtcaatga taaaattctg
     4861 catatccgtc acaaaacgtt ggccgtcttt ttcacggaaa cttggtttgt agccagctcg
     4921 ttcaaaacga cgaaagacct gttctgcacc atactgaacc acttctactg gcaatttaaa
     4981 agcacctagt ttttcgacca gcttgctttc atccaccacc caaatgtatt cttttgatgg
     5041 tgtcgcaaca accttctcca tcagaagagc accaccacca cctttgattc cattaaactg
     5101 actatccact tcatctgcgc catcaactgt cacatcgaca aagtctactt ggtcaataga
     5161 tttgagtggg atattaaggc cttctgcctg tttagtggtc acactagaag tcgttacagc
     5221 tataatctgc aatccttctt ccttgatacg acgaccgatt tcttcgacaa aataataggc
     5281 agtcgagcct gttccaagtc caacgaccat tccatccttg acaaactcag cagccttgat
     5341 acctgccatt ttcttcagat tttccactca aacacctcca ttaaagagct acctctatta
     5401 taccatgtaa aggcttttat ttcatctaaa agtaccttta gaatactgct tatttatcct
     5461 tgaaattaaa tttttttaat aaaagcagaa ctctgacaaa tcactttaag tacggatgta
     5521 aaccaaatca actctaacaa aaatagattt acaagagtaa aaaaacgaac aaatttagtt
     5581 tcttagcgag aagaatacta aacgttcgtt ttttgtgaat taagaataat gatacaaatt
     5641 atcaatattt tttgtctaac tattttagac atatcttttc attactttag gaagacaatt
     5701 actaatctcc gttggcaaaa ccacatactg ttcttgagct agttcttggg ctatggctga
     5761 atgaagatgg gtcgctactg ccacacgttc gtaaagacta gcttgcttaa actggcctgc
     5821 aaatcctgca atcatcccag caagcgtatc tcccattccc ccagtcgcct gatagggacc
     5881 gccaacctgt aactggtaat aatctgattg gccagcttgc caaatacgag tagctgaacc
     5941 tttctccacc aaaattgttc cttgagggaa ggaagttagg gcactagccg ttgcatcttc
     6001 cttttgcttt tcaatcgtaa ttccagataa tttttcccat tctttttggt gaggagttag
     6061 gataagctgg cttgaggaaa atgacaaact tgtcctagca agaatggtca aggcacctcc
     6121 gtctacaatc aaaatctgat tttgtcttaa gccagcaaag acctgtttaa ctagatcttc
     6181 tccaaaagca tcgtctcgta aacccggccc cagcaagaca acttctgcct tctccaattg
     6241 ctcttttaac aactgctggt cttgaagaga aaaggacata gcctcaggta aatggctgtg
     6301 cagggctgga atattttccc tatctgttcc aacggtcacc aatcctgcac cgctttttac
     6361 agctgccaaa gcagccatga tgatggcacc accataagga taagttccac caagaaacag
     6421 cagacggcca tagtctcctt tatgactagt acgagaacgt tcaataataa ctttttctag
     6481 taaggcttga tcaatcactt tcatcgttct tcctcaacta tcctttatct gattgtttta
     6541 aaacaacttg atagactttc ttagtccact cttctaagtc ttctccagca tagtcaagat
     6601 aatgcaccct atcttgtatt ttagttggaa taatattttc aaattggctc ttattatgag
     6661 ttggaataat acaaatatac aactcactaa ataaattcaa catgtcctta atttttgcaa
     6721 catggtagcc atcaaaaata taaccagtaa ctttaatcaa atctgtatag ataaaatgaa
     6781 aactcactcc aggaataaac ttgtcttgta attctttctc tgaaggagct cgacctaata
     6841 aacgctccat cgctaatcga taaccagaac ttgtatttga ccaccctaac atgacataat
     6901 caaaataatc tatcggatcc gatgcagcat ttctactatc caaaaccaac tcttcagcat
     6961 ctcgcctaaa ggctttcaca gcagataaga gctgacctgt tttaaagata gaaatcgctg
     7021 catctatagt cgtagtatga ctacagtagt ctgaagtcac taacctctta tgtagatcat
     7081 aagtcgaggc tactctttta tctgtagggg taaatggtgg cagaaaggga tgctgacaga
     7141 tgtaatctaa aatttctttt tttaaacaag aatcagcaca ctcaatactt gtttgacgtt
     7201 tatgaagttt ttcgtaagct ataaatttag caattttttc taattcccac tcttgaatat
     7261 ttggataatc aattagtgct aggtagtata ttccatccca ttgtaaacta tattccctat
     7321 tataaatttt taaaatcatc taatcacctc actccattat acaacaaaaa ggaggcgcag
     7381 acctcctttt gtaatcttat atctaaaatt taatattcat ttctgccatt ttaaatatag
     7441 ctatagaaaa tacactctct tcagcgattt ttctcttatt ttctatccaa tgtccgaagt
     7501 gctgcttgat aagtttgctc catcagcata gtaatggtca tagggccgac acctccaggg
     7561 actggcgtaa tatagctagc aagtggggca actgcctcat aatccacatc cccacagagc
     7621 ttaccatttt catcacgatt catcccaacg tcaatgacta ccgcacctgg tttgacaaag
     7681 tcagcagtca caaacttggc acgaccgatt gcgaccacca gaatatctgc tttagcagcc
     7741 accttagcaa gattatgggt gcgtgagtgg gtcaaagtca ctgttgcatt cttggccaaa
     7801 agaagctgag ccatgggttt tccaacgata ttagaacgac cgatgacaac cgcattttta
     7861 ccttctaatt caatcccata ttcatgaaac atttccataa ttcctgcagg tgttgatgga
     7921 atcatgactg gatgtccaga ccaaaggcgt cccatattta gaggatggaa accatccaca
     7981 tccttttctg ggtcaatagc caatagaacc gcctcttcat caatgtgttt tggtaatggc
     8041 aactgaacca aaatcccatg ccaagctgga tcctgattgt atttagcaat caggtctaac
     8101 aattcctctt gagtaatggt ctctggaact cgcactactt cgctacggga accagccgca
     8161 agagctgacc tctccttgtt gcgaacgtag acttggctgg ctggattatc cccaaccaaa
     8221 atcactacca aaccaggcac tagagctgtt tcttccttta attttgcagt cttttcagcc
     8281 aactgcccct gcaatttggc cgctaaagct ttcccatcaa taatctgtgt catatcactt
     8341 ctcttttctt tcaaatatct tccattatac caaaaactgc tagtgccctc taacacttct
     8401 ttcctaggtt atcctatagt ttcaaactat aagaaaaagc tctggttgaa cttttcacta
     8461 atcttgtctt gaaattttaa aatagattat aaaaccttac tcaagaaatc tttagtccgt
     8521 tgttcttggg tttgttcaaa aatctgctca ggtgtcccat cttcaacaac cacaccgtct
     8581 gccataaaga taacacgatc tgccacctca cgggcaaatc ccatctcatg tgttacgata
     8641 accatagtca tccctgactt ggcaaggtct tgcataacag ccaatacctc acctaccatt
     8701 tcaggatcca aggctgaagt tggttcgtca aagagcaaaa catctggttc catagccaac
     8761 ccacgcgcga tggcaatccg ttgttgctgc ccacccgaca gactctgtgg ataagcgtct
     8821 gccttatctg gcaaaccaac tttttccaaa agctcttggg ctctcttctc agcaacttcc
     8881 ctgctttcac ctttagtctt gataggtgac aaggtaatat tttccatcac agtcatatta
     8941 ggaaagaggt taaattgttg gaaaaccatg cccatcttct cacgcatggc aaacaggtca
     9001 ttcttcttgt ccgtaatatc gactccctca aagataagct tccccttggt tgcttcctcc
     9061 aacaaattca tagagcgaag caaggtagat ttcccactcc ctgaaggacc gatgataacg
     9121 acaacttctc ctcttttaat ctcgaggttg atgcccttca atacttcatt ctttccaaaa
     9181 gatttatgta aattttcaat ttttatcaag gtttctgtca ttatttctta tctccttgtc
     9241 ccatatgttt ttcaaaagct ttcaaggcta ctgtcagaat agaggtcata atcaagtagt
     9301 aaaatgctgc aaataaaagt ggtgttaaag gtagataggt tgttgtagaa actgttgtag
     9361 ccccattcca caactccatg accccaatag ctgataagag ggagctgtcc ttgataatgg
     9421 tgataaattc gttccccaat gctggcaaga tatttttgac tgcttgtggc aaaatcacat
     9481 aacgcatcgc atttttaaga cgaatcccta gcgaataagc cgcctctagc tgaccttttg
     9541 gaaccgcatt gattccggca cgaacagtct ccgaaacata agcaccacta ttcatagaga
     9601 taatcaaaat ccctggaatc agacgcgaaa aatcaacacc taaaattcca atctgaatag
     9661 tcggagcatt gatatgcata agagcaaagg caatcataat ttgaaccatc atcggtgtcc
     9721 cacggaaaat ccaaacgtac aagttggcca accaaacaag cggtttaaac tttgaacgtt
     9781 gcccaaaagc caagacaaca cccaaaatag ttcccaaaaa gataacacag atagaaataa
     9841 gaatcgtcac aacagcccca tagttaaaat aaggtaaata cttaggtaaa aaagaaaaat
     9901 tcatagtcac actgctttc
//
LOCUS       DAVMEI010000068         9757 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVMEI010000068 DAVMEI010000000
VERSION     DAVMEI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.068"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..168
                     /locus_tag="VYQ24_001236"
     CDS             <1..168
                     /locus_tag="VYQ24_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1968373.1"
                     /translation="LLCEEFGHKLLPLPPYSPEYNLIEKTWAHIKKHLKRVLPSCNTF
                     YEALLSCSCFN"
     gene            134..514
                     /locus_tag="VYQ24_001237"
     CDS             134..514
                     /locus_tag="VYQ24_001237"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1968374.1"
                     /translation="MRLFCLVLVSIDYINCLSETTDKNWHKSDRIFVTNTGKPVHSSI
                     LSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTSI
                     YTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(652..1956)
                     /gene="eno"
                     /locus_tag="VYQ24_001238"
     CDS             complement(652..1956)
                     /gene="eno"
                     /locus_tag="VYQ24_001238"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1968375.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2120..2566
                     /locus_tag="VYQ24_001239"
     CDS             2120..2566
                     /locus_tag="VYQ24_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1968376.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAKPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2563..3366)
                     /locus_tag="VYQ24_001240"
     CDS             complement(2563..3366)
                     /locus_tag="VYQ24_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1968377.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3375..3704)
                     /locus_tag="VYQ24_001241"
     CDS             complement(3375..3704)
                     /locus_tag="VYQ24_001241"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1968378.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(3649..4020)
                     /locus_tag="VYQ24_001242"
     CDS             complement(3649..4020)
                     /locus_tag="VYQ24_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1968379.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4079..5512)
                     /gene="glgA"
                     /locus_tag="VYQ24_001243"
     CDS             complement(4079..5512)
                     /gene="glgA"
                     /locus_tag="VYQ24_001243"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1968380.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYCWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSANITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5509..6648)
                     /gene="glgD"
                     /locus_tag="VYQ24_001244"
     CDS             complement(5509..6648)
                     /gene="glgD"
                     /locus_tag="VYQ24_001244"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1968381.1"
                     /translation="MKIDKYSAILGNTVGFHDMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVELGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6638..7780)
                     /locus_tag="VYQ24_001245"
     CDS             complement(6638..7780)
                     /locus_tag="VYQ24_001245"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1968382.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(7770..9698)
                     /gene="glgB"
                     /locus_tag="VYQ24_001246"
     CDS             complement(7770..9698)
                     /gene="glgB"
                     /locus_tag="VYQ24_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1968383.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELREKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         2942 a         2174 c         1729 g         2912 t
ORIGIN      
        1 ctcttgtgtg aagagtttgg gcataaactt ttacctcttc ctccctactc gcctgagtac
       61 aatcttattg agaaaacatg ggctcatatc aaaaagcacc tcaaaagggt attaccaagt
      121 tgcaatacct tttatgaggc tcttttgtct tgttcttgtt tcaattgact atataaattg
      181 tctaagcgaa acaaccgata agaattggca caaaagcgac cgtatttttg ttaccaatac
      241 aggaaaacca gttcatagtt ctatcttgag caagtctctc cagcgagcaa acgaacgcct
      301 taaaaaacca attcccaaac atctgtcccc tcacatcttc agacacacca ctattagcat
      361 cttatcagaa aataaaattc ctttaaaaac aatcacggac agggttggtc attccgactc
      421 tgaagtcact acttccatct acacccacgt cacaaagaac atgaaagatg aagcaatcaa
      481 tgtactggat aaagttatga aaaagatttt ttaaaaagtt ttgtcccttt tttgtcctct
      541 aaatacaaaa atagcccttc ggataaaatc cgaggggcta gaaacgttgt taaatcaacg
      601 gccgaacttt tgaatttcat ggttcgggat aaaatagttc actgaactat tttatttttt
      661 aaggttgtag aatgatttca atccacgata ttcagctact tcaccaagtt ggtcttcgat
      721 acgaagcaat tggttgtatt tagcgatgcg gtctgtacgt gaaagtgaac cagtcttgat
      781 ttgtcctgcg ttagttgcaa ctgcaatatc agcgattgtt gaatcttcag tttcacctga
      841 acggtgtgat acaacagcag tgtaaccagc ttctttagcc atttcgatag cttcaaaagt
      901 ttcagtaaga gtaccgattt ggttaacttt gataaggatt gagttagcag caccttcttg
      961 gataccacgt gcaaggtagt cagtgtttgt tacgaagaag tcgtcaccaa caagttgtac
     1021 tttcttacca agacgttcag taagagcttt ccaaccatcc cagtcgtttt catccatacc
     1081 atcttcaata gtgatgattg ggtatttgtt aaccaattct tcaaggtagt cgatttgttc
     1141 tgcagatgta cgaacagcag caccttcacc ttcaaattta gtgtagtcgt aaactttacg
     1201 ttctttatcg tagaattctg atgaagcaca gtcaaatccg ataaatacgt ctttacctgg
     1261 tacatatcca gcagcttcaa tcgcagcaag gatagtttca acaccatctt cagttccttc
     1321 gaaacgagga gcgaatccac cttcgtcacc tacggcagtt tccaaaccac gtgatttaag
     1381 gattttctta agagcgtgga agatttcagc accgtaacga agggcttctt taaatgttgg
     1441 cgcaccaact ggcaagatca tgaactcttg gaaagcgatt ggagcgtcag agtgagaacc
     1501 accgttgatg atgttcatca ttggagttgg aagaacttta gtgttgaatc caccaaggta
     1561 gctgtaaagt gggatttcaa ggtagtcagc agcagcacga gctacagcga tagacacacc
     1621 gaggattgca ttcgcaccca atttaccttt gttaggagta ccgtcaagtg caatcatagc
     1681 acggtcaata gcttgttgat cacgtacatc gtagccaatg atagcttcag caatgatgtt
     1741 gtttacgttg tcaacagctt tttgtgtacc aagaccaccg taacgagatt tgtcaccgtc
     1801 gcgaagttca actgcttcgt gttcaccagt agaagctcct gatggaacca taccacgtcc
     1861 gaaagcacct gattcagtgt aaacttctac ttcaagtgtt gggttaccgc gtgagtctag
     1921 gacttcgcga gcgtaaacat cagtaataat tgacattttt tactctcctt atgagttaaa
     1981 ttttttacac ctctataata ccttaaaacc cctccttttt caagaaaaaa cgttatcttt
     2041 gtgcaacttt tccttaactt tataaagtaa tcgctttctt ttgtctgttt tattctaact
     2101 tttatgatat actgttttca tgacagattt atcaaaacaa ttacttgaaa aagctcatgg
     2161 tgggttaaaa ataaatccgg atgagcaaag acgctatctt ggtacttttg aggaaagagt
     2221 tcttggatat gtagatattg acacagcaaa tagccctcag ttagaaaaag gctttttatt
     2281 tattttagaa aaccttcagg aaaaagcaaa gccactattt gtgaagattt caccaactat
     2341 cgaatttgat aagcaagttt tctacttaaa agaagcaaaa gaaactgata gtcaagccac
     2401 catagtatct gaagagcata ttacttctcc ttttggcctg gttattcata gcaatgcacc
     2461 agttcaagta gaagaaaaag accttcgact tgcttttcca aaactttggg aagttaaaaa
     2521 ggaagaacca gccaaaacat ccttatggaa gaaatggttt agctaaatct tgcacatatt
     2581 taataagtgc ccaatattgg cagccgtgcg ctccagatag agactggcat ttttcaaact
     2641 atcttctaaa ggttcacttt tctccaaaat agaaaagaca gcttggatat tttcaaatgg
     2701 taggggaggt aaatcttcag caagactacc gcaaatagca ataacaggaa ctccaacagg
     2761 ggttcttttt gcaacaccta taggcgcttt cccagcaaag ctttgactat caagtcttcc
     2821 ttctccaaca acaaccaagt cagcatctga aactttctta tcaaagttga ttaagtccaa
     2881 gcaggtatca attccagaca cgatacttgc ctgagcaaag gcacacaaac cagcagcaat
     2941 ccccagtcct ccatcattac tggccgtgcc accaacaccg atataaatat ctttaatccc
     3001 tttagaaatg agatggagaa tcaactctcc aataccacaa gtttggattt gaagtggatt
     3061 tcgtttctct agcggaattt ttccaagacc aaccaagtca gctacttcaa atagtgccag
     3121 ttcccctttt tgaaaatagc gcatggcttc tttttgtcca aaagggtctg tcacttggat
     3181 ccatttttct tttaggtcaa gagaatgtcg gatagcatct acagtacctt ctcccccatc
     3241 accaacaggg cagaggagac attctacatc tgctatcgat tgttggaagc ctctttttat
     3301 tgcttcagct acctgttgag ctgtcaagct ttccttaaac gaatccggtg caattacaat
     3361 cttcatattt tccctcattc taaacagtca atcaaaggaa gaacttctaa aaaatccctc
     3421 ttgtcaacat gatgtggtat ttcttttttg agcatttctt tggaacaaaa ggcgattcct
     3481 aactccgccg acttcaacat taatagatta ttaaccccat caccgattgc caccgttctt
     3541 tctttagaaa gttttagttt ctttctccat ttttccagag tttctttttt gacctgggga
     3601 cttataattt gtccaactaa ttttcctgtt aaaagacctt ctttgacttc aagctggttg
     3661 gcagtgaaat aggcaatacc aagggatttt gctaatctct ccaactattg gtgtaaatcc
     3721 accagacacc agaccaacta ggatgccatt cttttggaga atagagatga actctgggac
     3781 atttagcgat agatgaattg agttgaagac gttatcaaag accaaaatag gaagaccttc
     3841 caacaaggac actctttttc ttaaactgct ttcaaagacc aactctcctc gcattgctcg
     3901 acttgtaatc tgcgaaattt ccgcctcatg acctgcctct ctccctaaaa gatcaatcac
     3961 ttcttctagg attaaggttc catctacatc caaaacacac aagcctttta cttgagacat
     4021 cagttctcct ctctaaacag cctaaaaatc gtatgaagtc atcatacgat tttatctatt
     4081 aattaactaa actatggtac aagtcaaggt atgacttgca ggctgtatcc catgagaagt
     4141 cactctccat agcttgtttt tgtaggtttc tccaaatgtc tggatggttt ctatacaagt
     4201 ccaatgctgt ttggaaagtc caatttaacc aataaggaga tagattgtca aagctaaagc
     4261 cagtaccgct tccttcgatt ggattgaaag cgcgaactgt atctcgcaag cctccaactt
     4321 catggaccaa tggcaaggtt ccataacgca tagccatcat ttgagacaag ccacacggtt
     4381 caaaacgact tggcatgagg aagaggtcac aagcagcgta gatttcctga gcaagtttga
     4441 catcaaaagt gatatttgct gatagcttgt ctgggtaaat ctgagcaaac catgagaaag
     4501 ctccttcaaa ggctggatcg ccagttccca aaagaacaat ctgaacatct tcttgcaaga
     4561 tatggtgaag actttcgacc accacatcaa aacctttttg acgtgtcaaa cgagaaacaa
     4621 ttcccaccag tggaacgtct gctctaacag gcaagccaac tctttcttgc aattttgcct
     4681 tatttttggc tttcccagac aaatcttcct gattgaaatg atagtctaaa agagcatccg
     4741 tctgaggatt ataaagatca gcatcaatcc cattcacgat accagatact ttaccagact
     4801 ccattttaag aatctgatcc aaattacatc caaactgact agtcataatt tcatgagcat
     4861 agctaggtga aacggttgaa acacggttcg catagagaat acctgccttc atccagttca
     4921 gacagttgtt ccatcgaagg gtgccatcag cgtaacgttc aaggccaact ccaaacaaat
     4981 cacccaacat tccttctgaa aattgtcctt ggaattctaa attatgaatg gttaaaactg
     5041 tttcaatgtc ctcataggct tgaatccaac agtatttttc cttcaacaag aaaggaatca
     5101 tagctgtatg gtagtcatga acatggagaa gatcaggaat aaagtcaatc ctttccatag
     5161 cctcaatggc agccagttgg aaaaaggcaa agcgttctcc gtcatcaaaa tcaccgtaaa
     5221 catgaccacg gaagaaataa tattgattgt caataaagta gaaggttaca ccatttaata
     5281 ctgttttctt aattccacaa tactgtctac gccaaccaac gctcacctca aaatgaagca
     5341 catcttcaat ctgatttcca aatttagcct ctaccatatc atagtagggt aaaatcactg
     5401 caacttcgtg cccagctttt accagtgatt ttggaagagc gccaatgacg tctcccaaac
     5461 cacctgtttt tgaaaagggt gcaccctctg ctgctacaaa taaaattttc atgaatgaat
     5521 atcctctgtt actttagcac ctttcttaac cacaactgga tgttctgcag ttcctcgaat
     5581 cacaacacca agctcaactt caaccccttt gtccaagata gcatattcga cctgagcccc
     5641 ttctccaata acaacacgag ggaagagcag gctatcttta accaagctat ccttatggac
     5701 atgaatatta cgtgatagaa cagaattagc tacttgacct tcaataatac taccagaggc
     5761 aaactgagaa gtgcttacct tagatgtatt agcatagtaa gttggctctt cgtttttgac
     5821 ctttgtataa atcttttggt ttggtgagaa aagagaatag aatttttgtg attcaagcat
     5881 atcgatattc gcttgataat aagatttaac agagtgaata ttggctagat agcccgtgta
     5941 ctcgtaggca aaagctccct cttttacagc caaatcccgt aaaacatagc gcaatttctc
     6001 tggatgttct tttttagctt cttcttccaa gtgttcaatc aaccaaggtg tatcaacgac
     6061 aaagatatct gtagacatat tgaacgtttc agctgttgac ttgctatcaa agagtttatg
     6121 agaaagaaca tggtctgttt catctacatc caagattgca tttacttctg aaatatcttt
     6181 cttagctagt tttttataaa ctacagtgat aggctctttt gttgtactat gtaggtggaa
     6241 aacttggttc aaatcaatgt taataagaac atcgcagttg agggcaaccg tttggtttga
     6301 gccagaacgt ttcaaataag taagaagctg ttggtagtat tcttttccaa ctgtactact
     6361 ttctacacgg gtattgtaaa ttcctagata gtaatggcta agaagggttg ataagcccca
     6421 ctcgcgccct gaacgaatat ggtcaaagac tgagctgata ttatcctgct ggaaaatacc
     6481 aaagacacta cgaacacctg cattagcaag gcttgaaagt gggaagtcaa tcaaacgata
     6541 tttcccacca aatggcaaac ttgctactgg acggtggtcc gtcaatgtcg acatatcgtg
     6601 aaaaccaact gtatttccta aaatggcaga atatttatca atcttcatct gttgctaccc
     6661 ccactacttc attatatcct acaacttgta cttcatctgt tccatcaatt tcgacaccgt
     6721 cagaaataat cgcaccttca ccaataatgg cacgtttaat cttagctcct tgaccaatga
     6781 tagctccact catgataact gaatcaagga cttccgctcc ttcgcgaact tgcgcgcctg
     6841 ttgaaaggat agaatgttta acagttccat caacgaaaca tccgtctaca actaatgagt
     6901 cttccacatg agcatttgcc ccgaggaagt ttggtggtga aatcaagttt cttgagtaaa
     6961 tcttccattg acggttacga ctatccaagg cattttctgg agaaatatac tccatgttcg
     7021 cttcccaaag tgactcaata gtaccaacat ctttccaata accactaaat tcataagcat
     7081 aaacactttc acctgactca aggtaatttg gaatgacatt tttaccaaag tctgacatgc
     7141 caaccttgct cttttcagca gcgactaaca tattacgaag gcgttgccaa tcaaaaatgt
     7201 agattcccat agaagctttt gtagatttag gttgagctgg tttttcttca aattcaacaa
     7261 tacgattgtt agcatctgtg ttcatgatac caaaacggct tgcttcttta agagggacgt
     7321 ctaaaactgc tactgtcaag ctggcattat tatccttatg agactggagc atatcatcat
     7381 agtccatttt gtagatgtga tccccagaca aaatcaagac atactcagga ttgacactgt
     7441 cgatatagtc gatattttgg taaatagcgt gactagtccc ctcaaaccaa cgatttcctt
     7501 cacttgcaga ataaggttga agaatagaga cacctgaatt aataccgtct agtccccagc
     7561 ttgaaccatt cccaatatgg ttgttgagag caagtggttg atactgtgta acgaccccaa
     7621 cattatgaat ccctgagttg gcacagtttg atagggcaaa gtcaatgata cggtagcgcc
     7681 caccaaattg cacagctggt tttgcgatgc tttgagtgag tttaccgaga cgagttcctt
     7741 gcccaccagc aagaatcaaa gctaacattt catttttcat tttctactcc tttttggttt
     7801 ttatttgtga cggttttagt agatttcaag cgacgtttga ttttccatac acttgctccc
     7861 atagccggta gggtaaaggt taaggtctgc tcataatctt tccatagtcc ttcttgcgtt
     7921 tgaacagttt gattatgttc tttccaaacg cctccccact cttccaactc agtattccat
     7981 acttcttcgt aaattcctgc aacgggtagt ccgattgtaa aatctttccg ctcaacaggt
     8041 accatgttaa agatacagac taacatttct ccctttttac ccttacgaat aaaggaaaga
     8101 acactctggt ctcgattatc cgcatcaatg atttcaatac catcatagct ggtatcgatt
     8161 tcccacagac agcgatgatc tttgtaaaac tggtttagct gagaagtgaa atacttcatc
     8221 ttagcattca ttgggtcttc taggttagac cattccaact gttcttcaga tttccattct
     8281 aggaattgac cgtattcgct acccatgaag agcaatttct taccaggatg acaaatttgg
     8341 tacgtataga gattgcgcaa gcctgcgaat tgattgtaac gatctcccca catcttatgc
     8401 atcatactct tcttgccatg aaccacttca tcgtgcgaga atggcaagag ataattctcc
     8461 ttgaaaacat acataaagct gaaagtcacc aggttaaagt catatttacg atagatcgga
     8521 tcttcttcgt agaaacggag gatatcattc atccagccca tgttccattt gtagtcaaat
     8581 cctagaccac caatctcttt cattcccgta atcttggtcg cagacgaact ttcttctgca
     8641 atcatcatca catctggata ttctaactta ataacctcat tcaagcgctg aaggaaataa
     8701 taaccttcat agttgagatt tccgccatct ttattaggtg tccatggagc atcatcatag
     8761 tccaaataga gcatgttgct aacagcatcc acacgaatac catccaaatg atagacatca
     8821 atccaatgct taatgcaaga aattaagaag gactggactt cattttttcc aaggtcaaaa
     8881 ttaagggcac cccaaccatg gttatgagcc ttattatggt cttggtattc aaaagtcggt
     8941 gtcccatcat aataggctaa ggcatcatcg ttgatggtaa agtgacctgg tacccagtcc
     9001 acaataaccc caatattatg ggtatgacac tcctcgacaa aatcttgaaa ctcctctggt
     9061 cggccataag catgctctaa agcgaagtaa cccataagct gataccccca actcaagccc
     9121 aaaggatggg acatcaaggg cataaactca atatgagtat agttcatttc aacgagataa
     9181 ggaatgagtt catccttgag ctgggcaaaa ctataaggac tgccatcaga atttcttttc
     9241 catgatccag cgtgaacttc ataaatattg acaggacgct cttcaaagcc ccaacgtttt
     9301 cttcgtgcca gccaaagtcc atccttccat ttcttctcac gaagctctgt taagattgcc
     9361 cctgttcctg gacgagcctc atacctgaca gcaaaagggt caatcttcat cagttgatga
     9421 ccattttgac gtgtgacatg atatttgtaa atatgccctt cttgagccat attggtaaag
     9481 acttcccaga ccccaaaatc atttcttacc attggaatct gattttcaat ccagttggta
     9541 aaatcaccaa ccaagtgaac agcctgagca ttaggtgccc aaacacggaa ggtatagcca
     9601 tgctctccat ttagttcttc cctatgtgct cctagataat gttggagata aaaattttca
     9661 cccgtcataa aggtttttaa tgcttctcta ttatccatat actcctcttc ccctgtaagc
     9721 gttttctatg tttttattat actacctttt tagagaa
//
LOCUS       DAVMEI010000069         9633 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVMEI010000069 DAVMEI010000000
VERSION     DAVMEI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.069"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            26..370
                     /locus_tag="VYQ24_001247"
     CDS             26..370
                     /locus_tag="VYQ24_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968384.1"
                     /translation="MLDILLGTFLQISIVSIGWLVLYSGLFEAGVFLLANKGVAVKIK
                     EVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFIVFNALMYLLSS
                     SQVSLKKNKLSS"
     gene            422..1111
                     /locus_tag="VYQ24_001248"
     CDS             422..1111
                     /locus_tag="VYQ24_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1968385.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            1153..1386
                     /locus_tag="VYQ24_001249"
     CDS             1153..1386
                     /locus_tag="VYQ24_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968386.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1537..2148
                     /locus_tag="VYQ24_001250"
     CDS             1537..2148
                     /locus_tag="VYQ24_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1968387.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2309..3103
                     /locus_tag="VYQ24_001251"
     CDS             2309..3103
                     /locus_tag="VYQ24_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1968388.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3100..3735
                     /gene="trmB"
                     /locus_tag="VYQ24_001252"
     CDS             3100..3735
                     /gene="trmB"
                     /locus_tag="VYQ24_001252"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1968389.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            3861..4340
                     /gene="rimP"
                     /locus_tag="VYQ24_001253"
     CDS             3861..4340
                     /gene="rimP"
                     /locus_tag="VYQ24_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1968390.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4384..5520
                     /gene="nusA"
                     /locus_tag="VYQ24_001254"
     CDS             4384..5520
                     /gene="nusA"
                     /locus_tag="VYQ24_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1968391.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5542..5835
                     /locus_tag="VYQ24_001255"
     CDS             5542..5835
                     /locus_tag="VYQ24_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1968392.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            5828..6127
                     /locus_tag="VYQ24_001256"
     CDS             5828..6127
                     /locus_tag="VYQ24_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1968393.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6144..8936
                     /gene="infB"
                     /locus_tag="VYQ24_001257"
     CDS             6144..8936
                     /gene="infB"
                     /locus_tag="VYQ24_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1968394.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9187..9537
                     /gene="rbfA"
                     /locus_tag="VYQ24_001258"
     CDS             9187..9537
                     /gene="rbfA"
                     /locus_tag="VYQ24_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1968395.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         2936 a         1786 c         2216 g         2695 t
ORIGIN      
        1 attttttgtt attttcttga gtagtgtgtt ggatattctt ttagggacat ttttacaaat
       61 ctctatcgta tctattgggt ggcttgttct ttacagtggt ttgtttgaag caggagtttt
      121 ccttcttgct aataaagggg tggcggtaaa aatcaaggaa gtagatattc gaaatcgctt
      181 taaatttatt tttggaaaaa ccttatggtt tcaaattctt ttgctcatct ttttgataat
      241 caaactttat cttggtttgg atgcgaggtt gattttattc tatggacata ttttcattgt
      301 ctttaatgcc ttaatgtatc tattatctag tagtcaggtt agccttaaaa aaaacaaact
      361 gtcttcttaa tcttacagca gtagagcctg acaaggtgag gtcatagtag ggaggatttc
      421 tatgaaaaag tatcaacttc tattcaaaat aagtgcagtt ttctcttact tatttttcgt
      481 atttggtctt tctcagatga cgcttattat tcaaaattat tggcaatttt cttcccagat
      541 tggcaatttc gtctggattc aaaatttctt gagtttgcta tttagcggag tcatgatttg
      601 gattctggtt aagacaggtc atggttatct ctttcacatt ccaagaaaaa aatggctttg
      661 gtattcgatt ttgacagtat tagtggtagt gctccagatc tcttttaacg ttcagacagc
      721 taaacatgtt cagtcaactg ctgaaggttg ggctgtattg atcggttata gtgggaccaa
      781 ctttgctgag ctaggtatct atataacttt gttctttctg actccactta tggaagagct
      841 aatctataga ggattactgc aacacgcctt ttttaagcat tcgagatttg gccttgattt
      901 gcttcttccg tcaattttat ttgctcttcc tcatttttca agcctgccta gtttgttaga
      961 tatcttcatc tttgcaacat ctggcatcat ctttgctagt ttgacccgct ataccaagag
     1021 catttatcct tcctatgcgg tgcatgtgat caataatatt ttcgcaacat taccattttt
     1081 gctgactttt ttacataggg tcttggggta aaaaaacaaa aggcttgctt ttcagccata
     1141 gaggagatta tcatgtataa acacttattt ttcctagatt ccaaaacttt agatcggttg
     1201 acaccctata ttctagtctt ggcttctgac accattgctt ttaatgtttt tgtgctaacc
     1261 tttgtatctg cggtggtttt taatttccta aattccatgc tagctttaat ggctatattc
     1321 ataggggctg gctatgtggt cggattttgg ttactaatac tcaatgaaaa tcaaagagca
     1381 aactaggaaa ctagccgcag gctgtacttg agtacggcaa ggcgacgttg acgtggtttg
     1441 aatttgattt tcgaagagta taaaatggtt tgttttggaa aggctagaac taaagaatga
     1501 cgcgtaggaa gtctgtttgg tgaggaggat agttttatgg agttttttga taaatttcat
     1561 gccttgtgtt ttggattttt agtactaata attgtcatta cagttcctta tacgattaac
     1621 catgggggtt tttttcaaaa tgaatctgca ttgattcttg taagtcttct tgtaacctcg
     1681 ctgagtgttg cttatgctag aaagtttgaa atgatttctt ttgggatgtt aagcaagaaa
     1741 caacttttgc ttttcattgc aatctttctt ctaagtgtac ttgagacgct ggtttatatt
     1801 catttcttcg ctgtttcttc tggctcaggg gtccaacact tggcggaagt cagcagagga
     1861 atttccctgt ctttgatttt gactacctca gtttttggcc ccatccagga ggaactcatt
     1921 ttcagaggac ttcttcaagg tgcggttttt gacaattctt ggttagggct tgtgctaact
     1981 tcctctctct tttctttcat gcatggacct tctaatgtcc cttcgtttat tttttatcta
     2041 cttgggggtt tgttgctggg ctttgcttat aaaaagagtc aaaacctatg ggtttctact
     2101 ctagtccaca tgttttacaa cagttggcca ctcctatatt atttataaaa attatgaaaa
     2161 ggtaagcaga agatggtgct tacctttttc aagctataat gatacccaag ccaatccaga
     2221 ggcttttctt tgagaatcgg gttagagtgg ttgacgaata ggccaaaaac tagtagaata
     2281 gtaaggaaac tttatacgga ggaaagaaat ggatttgggt gataatgagc taacactgac
     2341 tcccatacct gggaaaagtg gcaaggctta tatgggtagc tatcctgacg ggaagcgcat
     2401 ctttgtaaaa atgaacacct ctccaatcct acctggtcta gctagagaac aaattgctcc
     2461 acaattatta tggagtcgtc gtttggcaga tgggcgtgat atgtgtgctc aagaatggtt
     2521 gacaggcaag atattgaccc cctatgatat gaatcgtaag caaatcgtca atattttaac
     2581 ccgtcttcat cgctcacgtc cgttgatgac acaattgagt cgtttgggct atgccatgga
     2641 aacacctgta gatttactac agtcttggca ggaaacggct ccagatgctt tgcgtaaaaa
     2701 tcattttatc agtgaagtga tggctgattt acgtcagact attccaggat ttagagagga
     2761 ccatgcgacc attgtccatg gagatgtacg acatagtaat tggattgaga cagatagtgg
     2821 cttgatttat ttagtagatt gggattcggt tcgcttgacc gatcgcatgt ttgatgtggc
     2881 ccatatgctc tgccattata tttcagaaca tcagtggaag gaatggttga cctactacgg
     2941 ttacaagtac aatcaaacgg tattaagtaa attgtattgg tatggtcaat tgtcttattt
     3001 gagtcagatt tccaagtatt atatgaacca agatttagaa aatgtcaatc gggagattca
     3061 tggtttgcgt catttccgag acaagtatgg aaagagaaga tgagagttag aaatcgtaaa
     3121 ggggcaacag aattactaga ggcaaatccc cagtatgtgg tcctcaatcc cttggaagcc
     3181 aaggcaaaat ggcgggactt gtttggcaat gataatccca ttcatgtgga agttggaagt
     3241 ggaaagggtg cctttgtttc aggtatggcc aagcaaaacc ctgacatcaa ctatatcggg
     3301 attgatattc aaaagtctgt tttgagctac gctttggaca aggtgcttga agttggagtg
     3361 cctaacatca agctcttgtg ggtagatggt tctgacttaa ctgactattt tgaagacggt
     3421 gagattgatc gcttgtatct gaacttttca gatccatggc cgaaaaaacg ccatgaaaag
     3481 cgtcgtttga cctacaagac cttcttggat accttcaaac gtatcttgcc tgaaaatgga
     3541 gaaattcatt tcaagacgga taaccgtggc ttgtttgagt acagtttagt gagcttttct
     3601 caatatggca tgaaactcaa tggtgtctgg ttagatttgc atgccagtga ttttgaaggt
     3661 aatgtcatga cagaatacga gcaaaaattc tcaaacaagg ggcaagttat ctaccgagtt
     3721 gaggcagaat tttaagagat agcctaaaat taggctgtac aagtgctttt gctttacata
     3781 agttggcaaa cgtgctatac tgatagtaag aatatgaaaa gtgaggcggg gaaatatctt
     3841 cgcctcttgc ttatgaggag gtggacgcaa tcgcaacaat cgtagaatta gtcagagaag
     3901 ttgtagaacc tgtcatagaa gctccttttg aactcgtgga tatcgagtat ggaaagattg
     3961 gcagtgacat gattctcagt atttttgtag ataaacccga aggaattacc ttgaacgaca
     4021 cggcagactt gacagaaatt atcagtcctg tcctagacac catcaagcca gatcccttcc
     4081 cagaacaata tttcctagaa attaccagtc caggtttgga acgccctttg aaaaccaagg
     4141 atgccgtcgc tggagcggtt ggaaaataca tccatgtcgg gctctaccaa gccatcgata
     4201 agcaaaaggt ctttgaagga accttgttgg ccttcgaaga ggacgagttg actatggaat
     4261 atatggacaa gacgcgtaag aaaaccgtcc aaattccata cagtttagta tcaaaagcac
     4321 gtttagcagt taaattatag aaaaagaaag gatagctttt gaggattcaa aagtgaagaa
     4381 aacatgagta aagaaatgct agaggccttc cgcattttgg aagaagacaa gggaatcaaa
     4441 aaagaagata tcatcgacgc agtagtagag tcgcttcgtt ccgcttatcg cagacgctat
     4501 ggtcagtcag acagcgtagc tattgacttc aacgaaaaaa caggtgactt tacagtttat
     4561 actgtccgtg aagttgttga tgaagtattt gatagccgtt tggaaatcag cttgaaagat
     4621 gctcttgcca ttaattcagc ttatgaactt ggagacaaaa tcaagtttga agaagcacca
     4681 gctgagtttg gtcgtgtagc agcccagtct gccaaacaaa ccatcatgga aaaaatgcgc
     4741 aagcaaacac gtgccatcac ttacaatact tacaaagaac atgagcaaga aatcatgtct
     4801 ggtacagtag aacgctttga caaccgcttt atctatgtca accttggtag catcgaagcc
     4861 caattgtcaa aacaagacca aattcctgga gaagtttttg cttctcatga tcgtatcgaa
     4921 gtttatgttt acaaggttga agacaaccct cgtggtgtga atgtctttgt tagccgtagt
     4981 catccagaaa tgatcaaacg tttaatggag caagaaattc cagaagttta tgatggaact
     5041 gttgaaatca tgagcgtggc tcgtgaagca ggtgaccgta cgaaggttgc tgttcgtagc
     5101 cacaatccaa acgtggatgc tatcggtaca atcgttggac gtggtggtgc taatatcaag
     5161 aagattacta gcaaattcca cccagctcgt tacgatgcta aaaatgaccg catggtacca
     5221 atcgaagaaa atatcgatgt tatcgagtgg gtagcagatc cagctgaatt tatctacaat
     5281 gccatcgctc ctgctgaggt tgaccaagtt atctttgatg aaaacgacag caaacgtgcc
     5341 ttggtggttg ttccagataa caagctttct cttgccattg gtcgtcgtgg acaaaacgtg
     5401 cgcttggcgg ctcacttgac tggttaccgt atcgatatca agtctgctag cgaatttgaa
     5461 gccatggaag acgctgcttc agtagagttg gaagtagaaa acgatactgt agaagaataa
     5521 aagctgctag aggagggaaa gatgaaaaca agaaaaatcc ctttgcgcaa gtctgttgtg
     5581 tctaacgaag tgattgataa gcgtgatttg ctccgcattg tcaagaacaa ggaaggacaa
     5641 gtctttattg atcctacggg caaggccaat ggccgcggcg cttatatcaa actagacaat
     5701 gcagaagccc tagaggcgaa aaagaagaag gtctttaacc gcagctttag catggaagtg
     5761 gaagaaagct tttatgacga gttgatcgct tatgtggatc acaaagtgaa aagaagagag
     5821 ttgggacttg aataagcaaa agataagtaa tctcttgggg cttgctcagc gagcagggcg
     5881 catcatatcg ggtgaagaat tggtggtcaa ggccattcaa gacggcaagg ccaagttggt
     5941 ctttctagct catgatgctg gacccaatct gaccaagaag attcaagata aaagtcatta
     6001 ttatcaagta gaaattgtaa ccgtgttttc aacactggaa ttaagcatag cagtcgggaa
     6061 atcgagaaag gttttggctg taacagatgc tggatttaca aagaaaatga ggtctcttat
     6121 ggaatagaag aggaggacat gatttgtcta agaaaagatt gtacgaaatc gcaaaagaac
     6181 ttggaaaaga aagtaaagaa gttgtagcgc gtgcaaaaga gttgggcttg gatgtgaaaa
     6241 gccactcatc aagtgtggaa gaagctgtcg ctgcaaaaat tgctgccagc tttaagcctg
     6301 cagctgctcc gaaagtagaa gcaaaacctg cagcaccaaa agtaagtgca gaaaagaaag
     6361 ccgaaaaatc tgagccagct aaaccagctg tagctaagga agaggcaaaa ccggctgagc
     6421 cagttgctcc gaaaacagaa aaagtagcag tgaaaccgca aagtcgtaat ttcaaggctg
     6481 agcgtgaagc ccgtgctaaa gagcaggcag aacgacgcaa gcaaaataag ggcaataacc
     6541 gtgaccaaca acaaaacgga aaccgtcaga aaaacgacgg ccgtaatggt ggaaaacaag
     6601 gtcaaagcaa ccgcgacaat cgtcgcttta atgaccaagc taagaagcag caaggtcagc
     6661 aaaaacgtag aaatgagcgc cgtcagcaag aggataaacg ttcaaatcaa gcggctccac
     6721 gtattgactt taaagcccgt gcagcagccc taaaagcaga gcaaaatgca gagtacgctc
     6781 gttcaagtga ggaacgcttc aagcagtatc aggctgctaa agaagccttg gctcaagcta
     6841 acaaacgcaa ggaaccagag gaaatctttg aagaagcggc taagttagct gaacaagcac
     6901 agcaagttca agcagtggtt gaagtcgtcc ctgagaaaaa agaacctgca gtggatacac
     6961 gtcgtaaaaa acaagctcga ccagacaaaa atcgtgacga ttatgatcat gaagaagatg
     7021 gtcctagaaa acaacaaaag aatcgaagta gtcaaaatca agtgagaaat caaaagaata
     7081 gtaactggaa taacaacaaa aagaacaaaa aaggcaataa caagaacaac cgtaatcaga
     7141 ctccaaaacc tgttacggag cgtaaattcc atgaattgcc aacagaattt gaatatacag
     7201 atggtatgac cgttgcggaa atcgcaaaac gtatcaaacg tgaaccagct gaaattgtta
     7261 agaaactttt catgatgggt gtcatggcca cacaaaacca atccttggat ggggaaacaa
     7321 ttgaactcct catggtggat tacggtatcg aagccaaaca aaaggttgaa gtggataatg
     7381 ctgacatcga acgtttcttt gtcgaagatg gttatctcaa tgaagatgaa ttggttgagc
     7441 gtccaccagt tgttactatc atgggacacg ttgaccacgg taaaacaacc cttttggata
     7501 ctcttcgtaa ctcacgtgtt gcgacaggtg aagcaggtgg tattactcag catatcggtg
     7561 cctaccaaat cgtggaaaat ggtaagaaga ttaccttcct tgatacacca ggacacgcgg
     7621 cctttacatc aatgcgtgcg cgtggtgctt ctgttaccga tattacgatc ttggtcgtag
     7681 cggcagatga cggggttatg cctcagacta ttgaagccat caaccactca aaagcagcta
     7741 acgttccaat catcgtagcc attaacaaga ttgataaacc aggtgctaac ccagaacgcg
     7801 ttatcggtga attggcagag catggtgtga tgtcaaccgc ttggggtgga gattctgaat
     7861 ttgttgaaat ctcagctaaa ttcaaccaaa atatcgaaga attgttggaa acagtccttc
     7921 ttgtagctga aatccaagaa ctcaaggcag acccaacagt gcgtgcaatc ggtacggtta
     7981 tcgaagcgcg cttggataaa ggaaaaggtg cggtcgcaac ccttcttgta caacaaggta
     8041 ccttgaatgt tcaagatcca atcgttgtcg gaaatacctt cggtcgtgtc cgtgctatga
     8101 ccaacgacct tggtcgtcgt gttaaagttg ctggaccatc aacaccagtc tctatcacag
     8161 gtttgaacga agcaccgatg gcgggtgacc actttgccgt ttacgaggat gaaaaatctg
     8221 cgcgtgcagc aggtgaagag cgtgccaaac gtgccctcat gaaacaacgt caagctaccc
     8281 aacgtgttag ccttgaaaac ctctttgata cccttaaagc tggggaactc aaatctgtta
     8341 atgttatcat caaggctgat gtacaaggtt ctgttgaagc cctttctgcc tcacttcaaa
     8401 agattgacgt ggaaggtgtc aaagtgacta tcgtccactc agcggtcggt gctatcaacg
     8461 aatcagacgt gacccttgcc gaagcttcaa atgcctttat cgttggtttc aacgtacgcc
     8521 ctacaccaca agctcgtcaa caagcagaag ctgacgatgt ggaaatccgt cttcacagca
     8581 ttatctacaa ggttatcgaa gagatggaag aagctatgaa agggatgctt gatccagaat
     8641 ttgaagaaaa agttattggt gaagcggtta tccgtgaaac cttcaaggtg tctaaagtgg
     8701 gaactatcgg tggatttatg gttatcaacg gtaaggttgc ccgtgactct aaagtccgtg
     8761 ttatccgtga tggtgtcgtt atctatgatg gcgaactcgc aagcttgaaa cactacaaag
     8821 acgacgtgaa agaagtgaca aacggtcgtg aaggtggatt gatgatcgac ggctacaatg
     8881 atattaagat ggatgatgtg attgaggcgt atgtcatgga agaaatcaag agataagatt
     8941 ttttgctcct ttcttaggtg gtgagggacg caagcaaacc gatggtttca ttgcttattt
     9001 ttgagcctag ggtctcaaaa atcccctgtg atgggactga taaatcagtt ccatcacttt
     9061 caccacggcg aaagaagcag atgacttcaa attgaacttc gtttcaattt aaactgaaaa
     9121 tcaagaagtt taaaatagct aggtctgctg gcctagcttt tggttcaaag tagagaaagg
     9181 aatatcatgg caaatcattt ccgtacagat cgtgtgggca tggaaatcaa gcgtgaagtc
     9241 aatgagattt tgcaaaagaa agtccgtgat ccacgtgtcc aaggtgtgac catcacagat
     9301 gttcagatgc tgggtgactt gtctgttgcc aaggtttatt acaccatttt gagtaacctt
     9361 gcttcggata accaaaaagc ccaaatcggg cttgaaaaag caactggtac catcaaacgt
     9421 gaacttggtc gcaatttgaa attgtacaaa atcccagatt tgaccttcgt caaagacgag
     9481 tccatcgagt atggaaacaa gattgacgag atgctacgca atctggataa gaactaaaga
     9541 agaggggttg cccctctttt ttggtggagg aaaataggtt gaatttgaaa tggaaaaata
     9601 ttcttttaaa atatattgaa actagaatag tac
//
LOCUS       DAVMEI010000070         9470 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVMEI010000070 DAVMEI010000000
VERSION     DAVMEI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.070"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..1700
                     /locus_tag="VYQ24_001259"
     CDS             27..1700
                     /locus_tag="VYQ24_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1968396.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEQEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            1812..2903
                     /gene="ulaG"
                     /locus_tag="VYQ24_001260"
     CDS             1812..2903
                     /gene="ulaG"
                     /locus_tag="VYQ24_001260"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1968397.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            3017..4993
                     /gene="tkt"
                     /locus_tag="VYQ24_001261"
     CDS             3017..4993
                     /gene="tkt"
                     /locus_tag="VYQ24_001261"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1968398.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            5110..5409
                     /gene="yajC"
                     /locus_tag="VYQ24_001262"
     CDS             5110..5409
                     /gene="yajC"
                     /locus_tag="VYQ24_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1968399.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            5455..5883
                     /locus_tag="VYQ24_001263"
     CDS             5455..5883
                     /locus_tag="VYQ24_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1968400.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNVSDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            5885..6295
                     /locus_tag="VYQ24_001264"
     CDS             5885..6295
                     /locus_tag="VYQ24_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968401.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILTVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            6595..9246
                     /gene="adhE"
                     /locus_tag="VYQ24_001265"
                     /gene_synonym="adhC"
     CDS             6595..9246
                     /gene="adhE"
                     /locus_tag="VYQ24_001265"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1968402.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         3028 a         1752 c         1978 g         2712 t
ORIGIN      
        1 tcaatagatt tgggggattt tttaatatgg tactggataa ggcaagttgt gatttacttc
       61 aatatttgat ggatcaagaa acgtccaaaa cgattatggc gatttcgaaa gatttgaaag
      121 agtcaagaag gaaaatttat tatcacattg acaaaatcaa tgctgctctg ggtgacgagg
      181 cgcttcacat cattagtatt ccacgaattg gtattcactt aacggaagag cagagagatg
      241 cttgttgtaa actattatcg gaagtagatt cgtacgatta tatcatgagt gcacatgaac
      301 gtatgatgat aatgttacta tggataggta tttctaaaga acgtattacg attgaaaaat
      361 tgatagagtt aacagaggta tctaggaata ctgttctcaa tgatttgaat agtattcgtt
      421 atcaactaac tttggaacaa tatcaggtga tcttgcaagt gagcaagtca cagggatacc
      481 accttcatgc ccaccctctt aataaaattc agtatcttca atcgcttcta tatcatattt
      541 ttatggaaga aaatgccact tttgtatcta ttttagaaga taagatgaaa gagaggttag
      601 atgatgagtg tttgctttct gttgaaatga accaattttt taaggaacag gttcctttag
      661 ttgaacaaga tttagggaag aaaataaacc atcatgaaat aacttttatg ttgcaggttc
      721 taccttattt gctgttaagc tgtcataatg ttgaacagta tcaagaaaga catcaggata
      781 tagagcaaga attttctttg ataagaaaaa gaatagagta tcaggtgtct aagaagttag
      841 gagaacggtt gtttcaaaag tttgaaattt ctttgtcagg acttgaagtt tctcttgtag
      901 ctgttctcct cctctcctat cgtaaagatt tggatattca tgcagaaagt gatgattttc
      961 ggcaattaaa acttgcttta gaagaattta tctggtattt tgaatcacaa atccgaatgg
     1021 agattgagaa caaggatgat ttgttacgaa atttgatgat ccactgtaaa gccttgttat
     1081 ttagaaagac ttacggtatt ttttctaaaa atcctctaac aaaacaaatt cgatccaagt
     1141 atggagaatt atttttagtc actagaaaat ctgcggaaat tttagaagga gcatggttta
     1201 ttcggctaac agacgatgat attgcctatt tgacgattca tattggagga tttttaaaat
     1261 atacaccatc gtctcaaaaa aatatgaaaa aagtttatct cgtttgtgat gaaggtgttg
     1321 cggtttcgag acttttgctg aaacaatgca aactttattt tccaaatgag caaattgaca
     1381 ctgtatttac aacagaacaa tttaagagtg tggaagatat tgcacaagtt gatgtagtga
     1441 ttactactaa tgatgatttg gatagcagat ttccgatttt aagggttaat cctatccttg
     1501 aagcagaaga tattttgaaa atgctagact atcttaaaca caatatattt cgtaataaga
     1561 gcaaaagttt cagtgaaaat ctttctagtc ttatttcgtc ttatattgta gacagcaagt
     1621 tggctagtaa gttccaagaa gaggttcaaa cacttataaa tcaagaaata gtagttcaag
     1681 cttttttgga agatatttga aggacagtcc aatgatgaac acaaacctgt gtttttcttg
     1741 gtctttttta gtgttttgaa gggtggtata ctaatctcaa agataacaat tatatccaaa
     1801 ggaggcaaca tatgccaaac gtcaaagaaa ttacaagaga gtcatggatt ttagccactt
     1861 tcccagagtg gggaacatgg ttgaacgaag aaatcgaaga agaagtcgta cctgaaggca
     1921 actttgccat gtggtggcta ggcaactgtg gtacttggat taagacacca gctggtgcta
     1981 acgttgtcat ggacctttgg tcaaaccgtg gaaaatcaac caaaaaagtg aaagatatgg
     2041 ttcgtgggca ccaaatggca aatatggcag gtgttcgtaa gttgcaacca aacttgcgtg
     2101 ttcagccaat ggttatcgat ccatttgcta tcaacgaact agactattac ttagtttcac
     2161 acttccacag tgatcatatc gacccataca cagctgcagc aattctcaac aatcctaagt
     2221 tagagcatgt taagtttatc ggtccttacc actgtggacg aatctgggaa ggatggggtg
     2281 ttccaaaaga acgtatcatc gttgttaaac caggtgacac tatcgaatta aaagatatga
     2341 agattcatgc agtagaatca tttgaccgta cttgcttggt aactctccca gtgaacggtg
     2401 ctgatgagac aggcggtgaa cttgctggct tggctgttac agatgaagaa atggctcaaa
     2461 aggctgttaa ctatatcttt gaaacaccag gtggaaccat ctatcatggt gcagattctc
     2521 acttctcaaa ctattttgca aaacatggta aagactttaa aattgatgtt gctttgaata
     2581 actatggtga aaatccggta ggtatccaag acaaaatgac atctatcgac cttcttcgta
     2641 tggcagaaaa tctgcgtacc aaagtcatta tcccagttca ctatgatatc tggtctaact
     2701 tcatggcttc tactaatgag attctagaac tttggaaaat gcgaaaagat cgcttgcaat
     2761 acgatttcca tccatttatc tgggaagttg gcggtaagta cacttatcct caagatcaac
     2821 acttagtaga ataccatcat ccacgtggtt ttgatgattg ttttgaacaa gactctaaca
     2881 ttcaatttaa agctttgcta taatataaaa atattctttg gaggttatcc gtatgttgca
     2941 tgatacggat aattttcata taatagtaaa atagaatgtg tgattcaata atcacctcaa
     3001 atagaaagga aattctatgt caaatctatc tgttaatgca attcgttttc taggtattga
     3061 cgccattaat aaagccaact caggtcatcc aggtgtggtt atgggagcgg ctccgatggc
     3121 ttacagcctc tttacaaaac aacttcatat caatccagct caaccaaact ggattaaccg
     3181 cgaccgcttt attctttcag caggtcatgg ttcaatgctc ctttatgctc ttcttcacct
     3241 ttccggtttt gaagatgtca gcatggatga gattaagagt ttccgtcaat ggggttcaaa
     3301 aacaccaggt cacccagaat ttggtcatac ggcagggatt gatgctacga caggtcctct
     3361 agggcaaggg atttcaactg ctactggttt tgcccaagca gaacgtttct tggcagccaa
     3421 atataaccgt gaaggctaca atatctttga ccactatact tacgttatct gtggagacgg
     3481 agacttgatg gaaggtgtct caagcgaggc agcttcatac gcaggtttgc aaaaacttga
     3541 taagttggtt gttctttatg attcaaatga tatcaacttg gatggtgaga caaaggattc
     3601 ctttacagaa agtgttcgtg accgttacaa tgcctacggt tggcatactg ccttggttga
     3661 aaatggaaca gacttggaag ccatccatgc tgctatcgaa acagcaaaag cttcaggcaa
     3721 gccatctttg attgaagtga agacggttat tggatacggt tctccaaaca aacaaggaac
     3781 taatgctgta cacggcgccc ctcttggagc agatgaaact gcatcaactc gtcaagccct
     3841 cggttgggac tacgaaccat ttgaaattcc agaacaagta tatgctgatt tcaaagaaca
     3901 tgttgcagac cgtggcgcat cagcttatca agcttggact aaattagttg cagattataa
     3961 agaagctcat ccagaactgg ctgcagaagt agaagccatc atcgacggac gtgatccagt
     4021 cgaagtgact ccagcagact tcccagcttt agaaaatggt ttttctcaag caactcgtaa
     4081 ttcaagtcaa gatgccttga atgttgtggc agctaaatta ccaaccttcc taggtggatc
     4141 agctgacctt gctcactcaa acatgactta tatcaagacg gatggacttc aagacgatgc
     4201 caatcgcttg aatcgtaaca ttcagtttgg tgttcgtgaa tttgcaatgg gaacaatctt
     4261 gaacgggatg gcccttcatg gtggacttcg tgtatacggt ggaactttct tcgtcttctc
     4321 tgactatgtg aaggcagctg tccgcttgtc agccttacaa ggacttcctg tgacttatgt
     4381 ctttacccat gattcaatcg cagttgggga agatggtccg actcacgaac cagttgagca
     4441 tttagcaggt cttcgtgcta tgccaaatct aaatgttttc cgtccagcag atgcgcgtga
     4501 aacgcaagca gcttggtacc ttgcagtgac aagtgagaaa acaccaactg cccttgtctt
     4561 gacacgtcaa aacttgactg ttgaagatgg aacagacttc gacaaggttg ctaaaggtgc
     4621 ttatgttgta tatgaaaatg cagccgactt tgataccatc ttgattgcga caggttcaga
     4681 ggttaatctt gctgtctcag ctgctaaaga attggctagt caaggcgaaa aaatccgcgt
     4741 agtcagcatg ccatctacag atgtctttga taaacaagat gcagcttaca aggaagaaat
     4801 tcttccaaat gcagtccgcc gtcgtgttgc agtcgaaatg ggtgcaagtc aaaactggta
     4861 caaatatgtt ggtctcgatg gtgccgttct aggtattgat accttcggag cctctgcccc
     4921 agcaccaaaa gtattggcag aatatggctt tactgtcgaa aatcttgtaa aagttgttcg
     4981 aaatttgaaa taatcctaaa aatcagggcg taagctctgg tttttcttac cagaaaagta
     5041 aggtacaatc ttgtaaaaga agctgaaatt tgatatagta gtcctatgta aaagacaaag
     5101 gagaaaacaa tgaatccaaa tattactttt ttaatcatgc ttgtaggtat gatggccttg
     5161 atgttcttta tgcaacgttc tcaaaagaaa caagctcaaa agcgtatgga aagcttaaat
     5221 aaactacaaa aaggctatga agtgattaca atcggtggac tttatggaac agtcgatgaa
     5281 gtagatacgg agaagggaac aatagttctt gacgtagatg gagtttactt gacttttgaa
     5341 ctagctgcta tcaagacagt attaccgctg aaagaaacag cttcactaga aggcgcaatt
     5401 gaaaaataag acgggatcac gaactcccgt tttctataaa agaaaggaaa tgggatgaaa
     5461 aaattagtct ttgtctgtct gggaaatatt tgccgtagcc ctatggccga gtttgttatg
     5521 aaatcaatga cagataacta cgaaatccaa agtcgagcaa cttcctcttg ggaacatggc
     5581 aatccgattc ataaggggac tcagggaatt tttcaagagt atgagattcc ttatgacaag
     5641 aacaagacat cgcttcagat tagtaaggaa gattttgaag cctttgatta tattatcgga
     5701 atggacgctt caaatgtttc cgacttacgt cagatgtgtc cagtagactg tcaagataag
     5761 atttactcat tttcatctga aagtgttcca gacccttggt atacaggaga ttttgaagaa
     5821 acctatcgac gtgttcaaga gggctgtcaa gcttggttag aacgtttaga aaaggagagt
     5881 taaggtggaa aatcttacga atttttacga aaagtatcgt gtttatctga ctcgtccacg
     5941 tttagagatt ttgacagtag ttaccattgt tttctgtgct gtactcgtct tttttctaaa
     6001 tattccagga aaaggtgtct taaaactcga taatggaacg attgtttatg atggcagtct
     6061 tgtccgcggt aaaatgaatg gccaaggtac cattaccttc caaaatggag accaatatac
     6121 aggtggcttc aacaatggag ccttcaacgg aaaaggtacc tttcaatcta aagaaggctg
     6181 gacctacgaa ggtgattttg taaatggtca ggctgaagga aaagggaaac taacaacaga
     6241 acaagaagtc gtttatgaag gaacttttaa acaaggcgtt tttcaacaaa aataaagcct
     6301 ccttatcaaa ggaggtatta ttagaattac aaggtaagcg cttacctgta aatccctttc
     6361 tttccaaatc cctcttccaa gcaagtttgt gaaataaaaa atatttgaaa taaatttcac
     6421 aaactttaaa gataaaacct gataagaaaa gaaaatgaga aaagtttcac aagagtttaa
     6481 aaattctttg tgaaatgttt atgaaactgt ttacaaagtt gaaataatgc gttataataa
     6541 acttgtgaaa aaattaacaa aggatatcga tccttgaaag ctatggagga aaatatggct
     6601 gataaaaaaa ctgtgacacc agaggaaaag aaactcgttg ctgaaaaaca cgtagatgag
     6661 ttggttcaaa aagctctagt tgcccttgaa gaaatgcgta aattggatca agagcaagtt
     6721 gactacatcg ttgccaaagc atcagtagca gctttggatg cccacggaga attggcttta
     6781 catgcctttg aagaaacagg acgtggtgta tttgaagata aagcaactaa gaacttgttt
     6841 gcttgtgaac acgtagtaaa caacatgcgc cacactaaga cagttggcgt tatcgaagaa
     6901 gacgatgtaa caggattgac tcttattgct gaaccagttg gtgttgtttg tggtattact
     6961 ccaacaacaa acccaacatc aacagcaatc ttcaaatcat tgatttcatt gaagacacgt
     7021 aacccaatcg tctttgcctt ccatccatca gcacaagaat catctgctca tgcagctcgt
     7081 atcgtccgcg atgcagctat cgcagctggt gctcctgaaa actgtgtgca atggattact
     7141 caaccatcta tggaagcaac aagtgccctt atgaaccacg aaggtgttgc gacaatcctt
     7201 gcaacaggtg gtaatgccat ggttaaggcg gcttattcat gtggtaaacc agctcttggg
     7261 gtaggtgccg gaaacgttcc agcttatgtt gaaaaatcag caaacattcg tcaagcagca
     7321 cacgatatcg tcatgtctaa atcatttgat aacggtatgg tctgtgcatc tgaacaagca
     7381 gttatcattg ataaagaaat ttacgatgaa tttgtagcag agttcaaatc ttaccacact
     7441 tactttgtaa acaaaaaaga aaaagctctt cttgaagagt tctgcttcgg cgtcaaagca
     7501 aacagcaaaa actgtgctgg tgcaaaattg aacgctgaca tcgttggtaa accagcaact
     7561 tggattgcag aacaagcagg atttacagtt ccagaaggaa caaacattct tgctgcagaa
     7621 tgtaaagaag ttggcgaaaa tgagccattg actcgtgaaa aattgtcacc agttattgca
     7681 gttttgaaat ctgaaagccg tgaagatggt attactaagg ctcgtcaaat ggttgaattt
     7741 aacggtcttg gacactcagc agctatccac acagctgacg aagaattgac taaagaattt
     7801 ggtaaagctg ttaaagctat tcgtgttatc tgtaactcac cttctacttt tggtggtatc
     7861 ggggacgttt acaatgcctt cttgccatca ttgacacttg gatgtggttc ttacggacgc
     7921 aactcagttg gggataacgt tagtgccatt aacctcttga atatcaaaaa agtcggaaga
     7981 cggagaaata acatgcaatg gatgaaactt ccttcaaaaa catactttga acgtgattca
     8041 attcaatacc ttcaaaaatg tcgtgacgtt gaacgtgtca tgatcgttac tgaccatgcc
     8101 atggtagagc ttggtttcct tgatcgtatc atcgaacaac tggaccttcg tcgcaataag
     8161 gttgtttacc aaatctttgc ggatgtagaa ccggatccag atatcacaac tgtaaaccgt
     8221 ggtactgaga ttatgcgtgc cttcaaacca gataccatca tcgcactcgg tggtgggtct
     8281 ccaatggatg ctgccaaagt aatgtggctc ttctacgagc aaccagaagt ggacttccgt
     8341 gaccttgtcc aaaaattcat ggatatccgt aaacgtgcct tcaagttccc attgcttggt
     8401 aagaagacta aattcatcgc gattccaact acatctggta caggatctga agtaacacca
     8461 tttgccgtta tctctgataa agcaaacaac cgtaaatacc caatcgctga ctactcattg
     8521 acaccaactg tggcaatcgt agatcctgct ttggtattga cagttccagg atttgttgct
     8581 gctgatactg gtatggacgt attgactcac gcgacagaag catacgtatc acaaatggct
     8641 agtgactaca ctgatggttt agcacttcaa gctattaaat tggtctttga aaatctcgaa
     8701 agctcagtta aaaatgcaga cttccactca cgtgagaaaa tgcataacgc ttcaacaatc
     8761 gctggtatgg cctttgccaa tgccttccta ggtatttctc actcaatggc ccataagatt
     8821 ggtgcgcaat tccacacagt tcacggacgt acaaatgcca tcttgcttcc gtacgttatc
     8881 cgttacaacg gtacacgtcc agctaagaca gcaacatggc ctaagtacaa ctactaccgc
     8941 gcagatgaaa aataccaaga tatcgcacgc atgcttggac ttccagcttc tactccagaa
     9001 gaaggggttg aatcttacgc aaaagctgtc tacgaactcg gtgaacgtat tgggatccaa
     9061 atgaatttta gagaccaagg aattgacgaa aaagaatgga aagaacattc tcgtgaatta
     9121 gccttcctgg cttatgaaga ccaatgttca ccagctaacc cacgtcttcc aatggtagac
     9181 catatgcaag aaatcatcga agatgcatac tatggctaca aagaaagacc aggacgccgt
     9241 aaataattgt ttatcagtct agaagcaaga caaaaactca atttgaggga aagatccagt
     9301 aatttttcta tgataaaagg catcctatca aggtttttga acacctgata ggatgccttt
     9361 ttatgatatt gaggcctttt tgcccttttt gaaaaactag aatagaaaca aaatatataa
     9421 tagattgaaa ctagagtagt acacatctgc ttctaaaaca ttgttagaat
//
LOCUS       DAVMEI010000071         9320 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVMEI010000071 DAVMEI010000000
VERSION     DAVMEI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.071"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            454..1497
                     /locus_tag="VYQ24_001266"
     CDS             454..1497
                     /locus_tag="VYQ24_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1968403.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1664..3751
                     /gene="topA"
                     /locus_tag="VYQ24_001267"
     CDS             1664..3751
                     /gene="topA"
                     /locus_tag="VYQ24_001267"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1968404.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3857..4216
                     /locus_tag="VYQ24_001268"
     CDS             3857..4216
                     /locus_tag="VYQ24_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1968405.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4309..4941
                     /locus_tag="VYQ24_001269"
     CDS             4309..4941
                     /locus_tag="VYQ24_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1968406.1"
                     /translation="MIYEFCAENMTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4985..5641
                     /locus_tag="VYQ24_001270"
     CDS             4985..5641
                     /locus_tag="VYQ24_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1968407.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5660..6292
                     /locus_tag="VYQ24_001271"
                     /pseudo
     CDS             5660..6292
                     /locus_tag="VYQ24_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
     gene            6320..6754
                     /locus_tag="VYQ24_001272"
     CDS             6320..6754
                     /locus_tag="VYQ24_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEV1968408.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTIDAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGCSVSYHDMPSV"
     gene            6766..7803
                     /gene="leuB"
                     /locus_tag="VYQ24_001273"
     CDS             6766..7803
                     /gene="leuB"
                     /locus_tag="VYQ24_001273"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV1968409.1"
                     /translation="MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAWPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDHILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVSMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7800..8069
                     /locus_tag="VYQ24_001274"
     CDS             7800..8069
                     /locus_tag="VYQ24_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1968410.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8073..8432
                     /gene="leuD"
                     /locus_tag="VYQ24_001275"
     CDS             8073..8432
                     /gene="leuD"
                     /locus_tag="VYQ24_001275"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1968411.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8594..9217
                     /locus_tag="VYQ24_001276"
                     /pseudo
     CDS             8594..9217
                     /locus_tag="VYQ24_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2877 a         1644 c         2069 g         2730 t
ORIGIN      
        1 tttagtgtaa ttgagaaagg agaaatgatt gtgattgatg ttggctaggt tatgttcaat
       61 gattcctacc gtctcaaatc ttgtcagtaa ggaaaaataa attcttcaaa agtagagatt
      121 acaaggcttg tttaagaaag aattcaaaga ccttgacaaa taaaaataaa atggttatta
      181 taaaaaatgg tctaaaatag atgatgatac ttttcgaaaa tctttttacg tcagctcagc
      241 tttgccttgc tgtgttttga gcaagctacg gttagcttcc gagtttgatt ttcatttact
      301 agaaatgaaa ctgatgagag atatcagtag acatttgagt caggatatta tggaaaatga
      361 taaaaagagc tcgtgagatt ggcatatcag actactaaag tattgagttt gttaggattt
      421 tagcgactag ttagctggga aaggaagata tttgtgacaa ataataaact gtattcgttg
      481 atagaattta gaaataaaat atatgaagaa ttagaacttt ccagaagtga tttagcgatt
      541 ttactatgtg ccatgcttat cgcctctatc ggattaaata tggattcgac tcccgtgatt
      601 attggagcca tgttaatctc tcctttgatg acacctattc tgggagtggg gctctctcta
      661 gctatatttg attttaaatt gttaagaaaa tcttttaaaa tattagctat tcaaattctt
      721 gccagtctaa tagcttcaac actttatttt tatctttctc ccatttcgta tgctagttcg
      781 gagattgttg ctagaacctc tccgactatt tgggatgttc tcattgcttt tgtaggaggg
      841 atagcaggta ttattggtgc taggaaaaaa gagaccaata atattgttcc tggtgttgct
      901 attgcaaccg ccttgatgcc tcctctttgt acagtaggtt atgctattgc ttctgctaat
      961 ctaaaattta tcataggctc ctcttaccta ttcctcatca attgtagctt tattgtcatt
     1021 gcgacttata taggtgttag gttgatgatg gttaagaaac attattttaa agataatgaa
     1081 gaagactcta aaatgcgtag gattttgctt ctagttgctg ttttgctgat gattccgagt
     1141 ttcatctctg caacgacttt agtgagagaa acgttgaaaa aagagtccct taagaaattt
     1201 atatcagagc agtttcaggg gtataatatt ttgaaaaaaa cctattctaa aaagactcat
     1261 accctaaagc taaccatttc aggaaattat ttgacagaag aagaactcga tatgatttca
     1321 agtaagagag gtgactatgg tttaagtgat gtttctgttc aagtttcaca attgtctgat
     1381 tcagaacaac ttagtaagga agagctggtg gagtatttct tccagtatat caaggataag
     1441 gaagcaaaag aaaaggagaa agccaataaa tttgatacag agtctgagga gcaataattt
     1501 cttgagaata gctggtttct ctcgtgagtc ttctatgtat atcaaaggaa gactgaggtt
     1561 ttaagtataa aacttttctt ctattatagt agctaagtct tgacagggtt caaaaaatgg
     1621 tttacacttt ataaagttta ttactttgaa aaggtgtgat actgtggcta cggcaacaaa
     1681 aaagaaaaaa tcaacagtta aaaaaaatct agtcatcgtg gagtcgcctg ctaaggccaa
     1741 gacgattgaa aaatatctag gcagaaacta caaggtttta gccagtgtcg ggcatatccg
     1801 tgatttgaag aaatccagta tgtccgtcga tattgaaaat aattatgaac cgcaatatat
     1861 caatatccga ggaaaaggcc ctcttatcaa tgacttgaaa aaagaagcta aaaaagctaa
     1921 taaagttttt ctcgcgagtg acccggaccg tgaaggagaa gcgatttctt ggcatttggc
     1981 ccatattctc aacttggatg aaaatgatgc caaccgtgtg gtcttcaatg aaatcaccaa
     2041 ggatgcagtc aaaaatgctt ttaaagaacc tcgtaagatc gatatggact tggtcgatgc
     2101 ccaacaagct cgtcggatct tggatcgctt ggtagggtat tcgatttcgc ctattttgtg
     2161 gaagaaggtc aagaagggct tgtcagcagg tcgcgttcag tccattgccc ttaaactcat
     2221 cattgaccgt gaaaatgaaa tcaatgcctt ccagccagaa gaatactgga cagttgatgc
     2281 tgtctttaaa aagggaacca aacaatttca tgcttccttc tatggagtag atggtaaaaa
     2341 gatgaaactg accagcaatg acgaagtcaa ggaagtcttg tctcgtctga ctagtaaaga
     2401 cttttcagta gatcaggtgg ataagaaaga gcgcaagcgc aatgctcctt taccctatac
     2461 cacttcatct atgcagatgg atgctgccaa taaaatcaat ttccgtactc gaaaaaccat
     2521 gatggttgcc caacagctct atgaaggaat taatattggt tctggtgttc aaggtttgat
     2581 tacctatatg cgtaccgatt cgactcgtat cagtcctgta gcgcaaaatg aggcggcaag
     2641 cttcattacg gatcgttttg gtagcaagta ttctaagcac ggtagcaagg tcaaaaacgc
     2701 atcaggtgct caggatgccc atgaggctat tcgtccgtca agtgtcttta atacaccaga
     2761 aagcatcgct aagtatctgg acaaggatca gctcaagcta tataccctta tctggaatcg
     2821 ttttgtggct agccagatga cagcggccgt ttttgatacc atggctgtta aattgtctca
     2881 aaatggagtt caatttgctg ccaatggtag tcaggttaag tttgatggtt atcttgccat
     2941 ttataatgat tctgacaaga ataagatgtt accggacatg gttgttggag atgtggtcaa
     3001 acaggtcaat agcaaaccag agcaacattt cacccaaccg cctgcccgtt attctgaagc
     3061 aacactgatt aaaaccttag aggaaaatgg ggttggacgt ccatcaacct acgcgccaac
     3121 cattgaaacc attcagaaac gttattatgt tcgcctggca gccaaacgtt ttgaaccgac
     3181 agagttggga gaaattgtca ataagctcat cgttgaatat ttcccagata tcgtaaacgt
     3241 gaccttcaca gctgaaatgg aaggtaaact ggatgatgtc gaagttggaa aagagcagtg
     3301 gcgacgggtc attgatgcct tttacaaacc attctctaaa gaagttgcca aggctgaaga
     3361 agaaatggaa aaaatccaga ttaaggatga accagctgga tttgactgtg aagtgtgtgg
     3421 cagtccaatg gtcattaaac ttggtcgttt tggtaaattc tacgcttgta gcaatttccc
     3481 agattgccgt catacccaag caatcgtgaa agagattggt gttgagtgtc caagctgtca
     3541 tcagggacaa attattgagc gaaaaaccaa gcgtaatcgc ctattctatg gttgcaatcg
     3601 ctatccagaa tgtgaattta cctcttggga caagcctgtt ggtcgtgact gtccaaaatg
     3661 tggcaacttc ctcatggaga aaaaagtccg tggtggtggc aagcaggttg tttgtagcaa
     3721 aggcgactac gaggaagaaa agattaaata agaggagggg cccgaaagta aatttcaggc
     3781 tccttttggt tagaacttga caaaatacat cattttatgc gaaactagaa gaagattatt
     3841 ttaactagga gaagttatgc gtcttatcta tctgataatt ggttttttat cactggcctt
     3901 ggctattgtt ggggttgttt tacccttgtt gccgacaacg cctttccttt tgttgtctat
     3961 tgcttgtttc tccagaagtt ccaagcgttt cgaagactgg ctttatcata ccaagctcta
     4021 tcaaacatat gtagctgatt ttcgcgagac taagtctatt acgcgtgaac gaaagaaaaa
     4081 aatcatcgtc tctatctacg tcttgatggg aatttctatt tattttgcac ctcttttacc
     4141 agtcaaaatc ggtctgggtg ctttgaccat ctttatcact tattatctct tcaaggtcat
     4201 tccagacaaa gaatagttaa aacagtagtt atttgccttg ataaaattga aagcatattc
     4261 ataacaatat gatataataa atttgaagta atattcaagg agaatcaaat gatttacgaa
     4321 ttttgtgctg aaaatatgac tttacttgaa aaagcgatgc aggctggagc tcgtcggatt
     4381 gaactctgtg ataatctagc agttggtggg acaacaccca gctatggagt gactaaggca
     4441 gcggttgaac tggcagctaa ctacgataca accatcatga ccatgattcg gccacgtggt
     4501 ggtgactttg tctataatga cctagaaatt gctatcatgc tagaagacat tcgtttgact
     4561 gctcaggctg gaagtcaagg ggttgtattt ggagctttaa ctgctgataa aaagttggat
     4621 aagcctaatc tggaaaagtt aattgctgca tcaaaaggaa tggaaattgt ctttcacatg
     4681 gcctttgatg aactaagtga tgaagatcaa ccggaagcta ttgactggct cagtcaagcc
     4741 ggtgtcactc gtatcctaac tcgtgctggt gtgtctggcg actccttaga aaaacgtttt
     4801 gttcactatc acagaatttt ggagtacgct aaaggtaaaa ttgaaattct accaggtggg
     4861 gggattgacc ttgaaaaccg tcaaaccttt atcgaccagg tgggggtaac acaattgcat
     4921 ggtactaagg ttgtttttta aaaaatagaa aggaactgct agcttttggt agcagttttc
     4981 acttatgttt gaaattttta aatcctatca atttaatcaa gaaaaggctc atgattatgg
     5041 ttttatagaa aatagcgaag tctggacata tagttgccag attttgcaag gtgactttgt
     5101 catgactgtg tccatcactg ctgataatgt gaactttcaa gtctttgacc aagagactgg
     5161 tgacctctat cctcacgttc atatggaaag catgagggga agttttgtcg gaaatgtccg
     5221 tgaggcttgt ctggagattc tttaccagat tcggaaggct tgttttgatg tgcaagattt
     5281 tatctgtcat cagactaagc gtatcatgac tcaagttcag gaaaagtatg gaaaccagtt
     5341 ggagtatctg tgggaaaaat cgcctgatac agctgtattg cgccatgaag gcaatcaaaa
     5401 gtggtatgcc gtcttgatga aaatctcttg gaataagctg gaaaagggca gagaaggaca
     5461 agtggaagca gtcaacctca agcatgacca agtagctaat ttgctttcac aaaaggggat
     5521 ttatccagcc ttccatatga gcaagcgcta ctggattagt gtgtcccttg atgatacttt
     5581 atcagatgaa gaagtactgg aattgataga aaaaagttgg aacttaacct ctaaaaaatg
     5641 aaatatttta ataattttca tgaactttca attagctaaa tattctttac tgaagaaatt
     5701 ttcagaaaat atagaattta ccacaccaga ggaatatggt gccatcttca aatacctgat
     5761 tgagaatgtc aagacggatc gtcagatcat ctattcgcct cactgtcatg atgaccttgg
     5821 aatggcagtg gcaaatagcc ttgctgctgt caagaatggt gcaggacgtg ttgaagggac
     5881 tatcaatggt attagggagc gagctggaaa tgctgctttg gaagaaattg cagtggctct
     5941 caatattcgc caagattact accaagtaga aaccagtatt gtcctaaatg agaccatcaa
     6001 tacgtcagaa atggtttctc gcttctctgg tattccagtt cctaaaaaca aagccgtcgt
     6061 tggtggcaat accttctccc acgaatctgg tattcactaa gatggagtcc ttaaaaatcc
     6121 tctcacttat gagatcatca cacctgaatt ggttggtgtt aagattccgc ttggaaaatt
     6181 atctggtcgc catgcttttg ttgagaaact gagagaattg gccctagatt ttacagaaga
     6241 ggatatcaaa tcactctttg ctaagttcaa ggcactggcc gataagaagt aagaaatcac
     6301 agatgcagat attcgagctt tggtagctgg aaccatggtt gaaaatccag aaggcttcca
     6361 ctttgatgat ttacaacttc aaactcatgc agataatgac attgaagcgc tcgttagcct
     6421 agccaatatg gatggtgaga aagtcgaatt taatgcgaca gggcaaggtt ccgttgaagc
     6481 aatctttaac gctatcgata agttctttaa ccaatctgtt cgtttggtgt cctacactat
     6541 cgatgcagta acagatggaa tcgatgccca ggatcgggtt ttggtcactg ttgaaaacag
     6601 agatacagaa accatcttta atgcagcagg gcttgatttt gatgtgttga aggcttctgc
     6661 tattgcctat ataaacgcta atacctttgt tcaaaaagag aatgcaggtg agatgggatg
     6721 cagtgtttct taccatgata tgcctagtgt gtaaaggaga aggctatggc aaagaaaata
     6781 gtagctctag caggagacgg aattggccca gaaatcatgg aggttggttt agaagttctg
     6841 gaggctctag ctgaaaaaac aggttttgac tatgagattg acagacgacc gttcggaggt
     6901 gcagatattg atgcagcatg gcctccctta cctgatgaaa cccttaaggc aagtagggaa
     6961 gcagatgcta tcctactagc agctatcggt agtcctcagt atgatggagc agtggttcgc
     7021 cctgaacaag gcctgatggc tctccgtaag gaactcaatc tttacgctaa tattcgtcct
     7081 gtaaaaatct ttgacagtct caagcatttg tcaccactca aactggaacg aattgctggt
     7141 gtagactttg tcgtggtgcg tgaattgaca ggcgggattt actttggaga tcatattctt
     7201 gaagagcgca atgcgcgtga tatcaacgac tatagctatg aggaagtgga gcggattatt
     7261 cgcaaagcct ttgaaattgc aagaaatcgc agaaaaatcc ttactagtat cgataagcaa
     7321 aatgttctag cgacctcaaa actctggcgg aaagtagctg aggaagtcgc acaggatttc
     7381 ccagatgtaa ccttggaaca ccagctggta gactcagctg ctatgcttat gattaccaat
     7441 cctgctaagt ttgatgttat tgtaacggag aatctttttg gagatatttt atctgatgaa
     7501 tcaagcgtct tatctggtac acttggggtt atgccatcag ccagtcattc cgaaaatgga
     7561 ccaagtctct atgaacctat tcacggttca gcacctgata ttgcaggtca aggaattgcc
     7621 aatcctattt ccatgatttt atcagtttcc atgatgttga gagatagttt cggacgttat
     7681 gaggatgcag agcgtatcaa acgtgctgtt gagacaagtc tggcggcagg aattttaacg
     7741 agagatatag gaggtcaggc ttcaacaaag gaaatgacgg aagctattat tgcaaggtta
     7801 tgaagttaga cgaaaaaatt actctagtcc ttttgatttg gaatgtcatc attttcttga
     7861 tttatggcat tgacaaattc aaggcaagaa gaagagcttg gcgcatccaa gagaaaatct
     7921 tactcatttt agcccttact tgtggtggtt ttggggcctg gttagctgga atcacttttc
     7981 accacaagac tcgaaaatgg tactttaaaa cagtttggtt tcttgggatg gtgaccacac
     8041 tagtagcctt atattttatt tggaggtaat ggatggcagg gtcttcgagg gaacacgctg
     8101 cttgggctct agcggactat ggttttaagg tcgtgattgc aggatctttc ggtgacattc
     8161 attacaataa tgaactcaat aatggcctgt tgccaatcgt tcagcctaga gaggttagag
     8221 agaaactagc ccagctaaaa ccaaccgacc aggtaactgt ggacttggaa caacaaaaaa
     8281 tcatctcacc agttgaagaa ttcaccttcg agatagatag cgagtggaaa cataaactcc
     8341 taaatagttt ggatgatatc ggtattacct tgcagtatga agagttgatt gctgcttatg
     8401 aaaaacaacg accagcctac tggcaggatt agaaaaaata gaaaaggaaa tatagtaaac
     8461 tgaaataaga tgtaaacaaa tgaattagag cttaacatcc atttccagca attttttaga
     8521 aactacagtg gactattctg gattcaacac attataaaat tatgacaaaa cacattcaca
     8581 agaaggctac gacattttaa aaggtgaggg cggatgtatc gtttgcccta ctaaagttgg
     8641 ttacattatc atgaccagca acaaggcagg acttgagcgt aagttcgcag ccaaagaacg
     8701 taagcgtaac aaaccaggtg ttgttctctg cggtagcatg gatgaacttc gcgctttagc
     8761 gcaactcaac ccagaaattg aagcattcta ctaaaaacat tggatgaaga tattcttctt
     8821 ggttgtatcc ttccttggaa accagaagcc tttgaaaaac tcaaagcata cggggatggc
     8881 cgtgaagaac ttatgactga tgtacgtggt actagctgtt ttgttatcaa gtttggaaaa
     8941 gcaggtgaac aattggctgc caagctttgg gaagaaggta aaatggtcta cgcctcatat
     9001 gcttcaatga caaaacgatt gaaactcgct atgagagcaa ggtgtaatgg tgtctatggt
     9061 cgataaggac ggcaaactca tcccagaaca aggaggagca cgttcaactt caccagcacc
     9121 agttgtgatc cgtaaagggc ttgacattga taaaatcatg atgcacctgt cagatacttt
     9181 taactcatgg gactaccgtc aggttgagta ttattaagat aaaaaaagaa gtctagtgtt
     9241 atgagatatt aaagctccta acactggact ttttgtttag aatttctttt ctttttctat
     9301 aggatatggt attctatgta
//
LOCUS       DAVMEI010000072         9298 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVMEI010000072 DAVMEI010000000
VERSION     DAVMEI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.072"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            12..1232
                     /locus_tag="VYQ24_001277"
     CDS             12..1232
                     /locus_tag="VYQ24_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1968412.1"
                     /translation="MLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILYEKDATQP
                     VEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPMEARNYTVE
                     ELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTTGLNNETL
                     GDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTITSTNYML
                     EGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPYARFTATS
                     SLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERVGNQSSQS
                     VQFTPDSKAIPAPLEAGTVVGHLTYEDKNLIGQGYITTERPSFEMVADKKIEKAFFLK
                     VWWNQFVRFVNEKL"
     gene            complement(1463..2875)
                     /gene="sufB"
                     /locus_tag="VYQ24_001278"
     CDS             complement(1463..2875)
                     /gene="sufB"
                     /locus_tag="VYQ24_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1968413.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(2929..3369)
                     /locus_tag="VYQ24_001279"
     CDS             complement(2929..3369)
                     /locus_tag="VYQ24_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1968414.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQTSLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            complement(3356..4582)
                     /locus_tag="VYQ24_001280"
     CDS             complement(3356..4582)
                     /locus_tag="VYQ24_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1968415.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILAEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            complement(4593..5855)
                     /gene="sufD"
                     /locus_tag="VYQ24_001281"
     CDS             complement(4593..5855)
                     /gene="sufD"
                     /locus_tag="VYQ24_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1968416.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGDDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            complement(5883..6653)
                     /gene="sufC"
                     /locus_tag="VYQ24_001282"
     CDS             complement(5883..6653)
                     /gene="sufC"
                     /locus_tag="VYQ24_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1968417.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            complement(6803..6997)
                     /locus_tag="VYQ24_001283"
     CDS             complement(6803..6997)
                     /locus_tag="VYQ24_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1968418.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            complement(7025..8680)
                     /gene="dnaX"
                     /locus_tag="VYQ24_001284"
     CDS             complement(7025..8680)
                     /gene="dnaX"
                     /locus_tag="VYQ24_001284"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1968419.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            complement(8680..9177)
                     /locus_tag="VYQ24_001285"
     CDS             complement(8680..9177)
                     /locus_tag="VYQ24_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1968420.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
BASE COUNT         2712 a         2080 c         1754 g         2752 t
ORIGIN      
        1 tatttttaac tttgttaact gtctctcttt tagggggtgc ttctactgct gttgctcaag
       61 attttaccat tgccgctaaa catgcgattg ctgttgaggc aaatactggt aaaattctct
      121 atgagaagga tgcaacgcaa cctgtcgaaa ttgcttccat aacaaaattg attactgttt
      181 atctggtcta tgaagctttg gaaaacggaa gtattaccct ctccactcct gtagatattt
      241 ctgattatcc ttatcaattg acgacaaatt ctgaagccag taatattcct atggaggccc
      301 gtaattatac tgtcgaagag ttgcttgaag caactctggt atctagtgcc aacagcgccg
      361 ctattgccct agctgagaaa attgctggct cagaaaaaga tttcgtcgat atgatgcggg
      421 caaaactctt ggaatgggga attcaggatg ccactgttgt caatacgaca ggtcttaaca
      481 atgaaactct aggggataac atttacccag gttctaaaaa agatgaggaa aataagctta
      541 gtgcttatga tgtcgctatc gttgctcgca acctcatcaa aaaataccca caagtcttag
      601 aaatcaccaa aaaaccttct tctacttttg ctgggatgac aatcacttca accaactaca
      661 tgttagaagg tatgcctgct taccgtggtg gttttgatgg gctaaaaaca ggaacaacag
      721 ataaggctgg agagtctttt gttggtacta ctgtcgaaaa aggcatgaga gtcatcacag
      781 ttgttttaaa tgcagatcat caagacaata atccttacgc tcgatttaca gctacatctt
      841 ccctaatgga ttatatttct tctacattta cacttcgcaa aatcgttcaa caaggcgatg
      901 cctatcaaga tagcaaagcc cctgtacaag atggaaaaga agatacagta actgcagtgg
      961 ctccagagga tatctatcta atagaacgtg ttgggaatca atcttcccaa tctgttcaat
     1021 tcacacctga ttccaaagca atcccagcac cacttgaagc tggaacagtg gttggccatt
     1081 tgacttatga agacaagaac ttgattggtc aaggttacat caccacagag cgccctagtt
     1141 tcgaaatggt agcagacaag aaaattgaaa aagccttctt cttaaaagtt tggtggaatc
     1201 agtttgtccg ctttgttaac gagaaattat aaaaaatcgc gattttaaac cgcgattttt
     1261 tgatacaagt tcatcttcaa aatccttgat tttgagtaaa tctaatactc aaaatcaaag
     1321 aataaactag gaagctagcc gcaggttgct caaaacactg ttttgaggtt gtagatgaaa
     1381 ctgacgaagt cagtaaccat acatacggta aggcgatgct gacgtggttt gaagagattt
     1441 tcgaagagta taaaatcaaa ttttatccaa ctgatccctc catttcatag ctaatcaagc
     1501 ggttcagctc aactgcgtat tccattggaa gttcttttgt aaagggttct acaaatccca
     1561 tgacaatcat ttcagttgcc tcagattctg acaatccacg gctcatgaga taatacaatt
     1621 gctcttctga aatcttagat actttggctt cgtgttccaa agccacttgc gagttgtgaa
     1681 tttcattaaa tggaatagta tctgatgctg acaagtcatc catgataatg gtatcacatt
     1741 caatgtggga aacagatttc ttagagttct tgttaaaggt gacttgtcca cggtagtcaa
     1801 cctttcctcc acctttagcg atggatttag acacaataga cgagctggta tgtggagcat
     1861 tgtgaatcat cttagcaccc gtgtcttggt gttgccctgc attagcaaag gcgatagaga
     1921 gcatggtacc acgcgctcct tctccatcaa ggtaaacaga tggatatttc atagtcgttt
     1981 tggcacccaa gtttccatca atccactcaa cagtggcatc cttttgagcc ttagcacgct
     2041 ttgttaccaa gttatagacg ttatcagacc agttttggat agttgtataa cgcatataag
     2101 ctccgtccaa agcaaaaatt tctacaatgg cagcgtgtaa gctattgctt gaatatgttg
     2161 gtgctgtaca tccttctacg tagtggacgc ttgctccctc atcaacgata atcaaggtac
     2221 gttcgaactg acctatattt tcgttattga tacggaaata agtttgaagt ggaatatcta
     2281 ccttgacacc ttttggcacg tagataaaag ttccacccga ccatactgct gagttgaggg
     2341 ctgccaactt gttatctgtc ggcggtacca acttcgcaaa gtattgttta aataagtctg
     2401 ggtattcctt gagtgcggaa tctgtatctg taaagataat acctaatttt tggaactctt
     2461 ccttcatgtt gtggtaaacc acttctgact cgtactgggc agaagcccct gctaaataag
     2521 cacgttcagc ttctggaatc ccgatacgtt caaaggtttc tttaatcttt tcaggtacat
     2581 catcccaaga acgggctggt ttgtcagatg gtttttggta gtagattaag tcatcaaagt
     2641 caatctctga caagtctgct ccccaagttt gcatgggcat ttttttgaag gtttcataag
     2701 acttcaaacg gaactccaac atccactcag gctcaccctt agcagcagat aattcacgaa
     2761 taacaccttc gttgagtcct tttcctgtcg ataagacagg ctctacatcg tcatggaaac
     2821 caaatttata ttcaccaagg tcaattggtt ttggttctac tctttcttca gccatagtat
     2881 cctttctttc ttcatttcta ataattcata agacaaaaga aacttgtctt actgtttttc
     2941 ttgattttca attgttttct taagggcatt ccaagctagg gttgcacact tgattctttg
     3001 agggaatttg gcaacacctg acaagaatgc cgcgtctcca agttggcctt gacgctcatc
     3061 tttttgcccc tgaaccattt cagaaaaaat agtcgccagt tctaaaattt cttgtttggt
     3121 ttttcctaaa acggcatctg tcatcatact agcagaagca gttgaaatcg tgcatcctga
     3181 atttagaaaa gcaatatctt ccaaacggtc ctctgcatca aacttgacag agaggttgat
     3241 gacatcccca caagtcggat tgttgagact ggtttgctca gcatcttcta acttcccttg
     3301 gtgatgtgga tttttcgaat ggtctgctac cactgccata taaaggctat ctagtttaga
     3361 aagtgccatt gaaaaactcc tttgtctttt gtagggcatc gactagtttg tcgcaatctg
     3421 ccttggtatt gtagatataa aaacttgcac gagctgttgc tgggacttcc aaatactgaa
     3481 gcaagggttg cgcacaatgg tgaccagcac gaacagccac tccttcataa tccagagccg
     3541 tcgcaagatc gtgaggatgg agatcaccta ggttaaaggc aataacaccc gaacgttgag
     3601 ccaaatcctg agaaccgtaa atggtcaatc cctcaattgc ctgcagtttt ggatagacgt
     3661 acgcaatcaa ttcctgttca tgagcttcaa cggcatccat accaatcttt tccagataat
     3721 caactgcagt cgcaagtcca atagctcctg ccatatttgg cgttccagcc tcaaatttcc
     3781 aaggcaattc cttccaacta gcaaattgct cgtagacaaa atcaatcatc tcgccgccaa
     3841 attctactgg agacatttgc tcaagatact tttctttgcc gtaaaggaca ccgataccag
     3901 tcggaccagc catcttgtga cccgaaaagg cgaaaaagtc cagatccaag tcctggacat
     3961 caatcttcat atgaggtgta gattgagcac catccactac cataattgcc ccaacttggt
     4021 gggctaattg agtgatttcc ttgatcggat tgaccacacc aagaacattg gaggcatgag
     4081 ctagggaaac aaatttaacc ttatcagtca atttagctcg caaatcctcc atatccaagg
     4141 caccgtcttt aagatagaca tagacaagct ctgctccagt ctttcgacaa gcttcctgcc
     4201 atggaatgat attagaatgg tgttccatta ctgaaatcaa gacctggtct ccctcagcga
     4261 gaatttcctc agcaaagcgt gccacccagt taaggctggt tgtcgttcct ctggtaaaga
     4321 gaacttcctt tgtagagcct gcattaataa acttacgaat ggtttcacga gcagcttcat
     4381 aagaagctgt cgctcgttcc gctaaggtat ggacaccacg gtgaacattg gcattgtcct
     4441 gctcatagta gctgttaata gctttcagaa ctactagtgg tttttgtgtc gtcgcagcat
     4501 tgtccagata gaccagaggt tcatcattga caatctgatc taaaattgga aaatccttgc
     4561 gaatcgcttc tacatctaac ataggcttcc ccttagcgtt ttgacaattt ctcttcgata
     4621 gttgcaatca tttcatcacg aacttccttg actggaatct ccacgataac agatccaagg
     4681 aaaccacgaa caaccaaacg ctctgcagtt gccttatcca agccacgact catgaggtaa
     4741 tacatgtctt ctggatctac ctgaccaata gaggctgcat gtcctgcagt tacgtcattt
     4801 tcatcaatca aaagaattgg gttagcatct gaacgcgctt ggtctgaaag cataagaaca
     4861 cggctctctt gttgcgcatc tgctccctta gcacccttga tgatgtggcc gataccattg
     4921 aaagtcaaag ttgctttttc aaggataacc ccatgttgta ggatatttcc gatagagttg
     4981 cagccatagt tagttacacg agtatcaatc ccttgtacct gacgaccact tgaaagagct
     5041 acaaccttca ggtcagcatg gctaccatta ccaatcaagt cactatcaaa atcagcaacg
     5101 acatttcctt cgttcatgac accgatagcc cagtcaatac ttgcatcgtc gcctaattta
     5161 ccacgacggc taatgtaggc agtgacgttt tcacctagac ggtcgatagc agcaaacttg
     5221 acttgcgcac cagaacgtgc aatcacttcc actgtgatat tggcagttgc tttgtcactt
     5281 ccttcaccgc gtgactctaa acgctccaga taactaatct tagaattttt accagcgata
     5341 atcataatat gcttgttaaa cggcacattg ctatcgctat cttggtagaa aattccttca
     5401 attggctctg taatttctac gttatctgga atatagagta cagcaccact gttaaagtaa
     5461 gctgtgtggt aagccgccaa cttgtcatca tcatacttaa cagatgacat gaagaattct
     5521 tcgatcagct ctggaatttc ttctaaagct gagtgaaagt ctgtgaagac aacaccctgt
     5581 tcagctaact caactggagt ttgttcgaaa acagtttgag ttcctacttg caccaacttc
     5641 aagtgatgat ctaaagctgt gaaatctgga acatttgctg atggctcatt ttctgtaatc
     5701 gttccatcac ccagattcca acggtggaat ttgacacgct caataactgg taattccaaa
     5761 gtctcaatct tgtcaaaagc tttttgacgg agatcagcca accagcttgg ttcagcgtgc
     5821 atttctgaaa aaagtttaat attttctcta gtcatttact tctcctaaaa gatacgaggg
     5881 aattacaatt cttccttgta gtcgtagcca agttcttcag ctagttttgc gtatccttca
     5941 cgttccaaac gcgcagccaa ttctggacca ccagaaagga caacacgacc ttccatcatc
     6001 acgtgtacca catcaggtgt gatatagttc aaaagacgtt ggtagtgagt aatgatcata
     6061 gcaccaaaac cttcaccacg catggcattg acacctttag acacaacttt aagagcgtca
     6121 atatcaagac ctgagtcaat ctcatccaaa agagcaaatg ttggctccaa catcaaaagt
     6181 tgaagaattt cattgcgttt tttctcacca ccagagaagc cttcgttgag gtaacgctct
     6241 gccatttctt ctttcatgtt gagcaattcc attttttcat ctagcttagt aataaactca
     6301 cgaactgaaa tcttctcatc atcttcttta cccgcattca tagcggcacg aagaaactca
     6361 gcattggtaa ttccagggat ttctgatggg tattgcatag caaggaaaag tcccatacgc
     6421 gcacgctcat ccacttccaa ctcaaggatg tttacgccat caaacaaaac ttcaccttta
     6481 gttacttcat agtttggatt tcccatgata gcggcagaaa gagtcgattt acctgtacca
     6541 tttggtccca tgatagcggc aatttctcct gttttcaggg tcaggttaac cccttttaaa
     6601 atttcttttc cttcaatctc aacatgaaga tctttgatct ctaatactga catgatagtt
     6661 cctttctttt tcaaaacttt tacagtactt actagaaaaa cgtctgattt ccccaaaaat
     6721 agagcaaaaa ttctataaat atacttttct agtataacaa aaaaaagcct aaaaggcttt
     6781 gattttgtct ggaagctgag ggctagtctt tcctattaaa atgctgatcg atgacatcaa
     6841 cgattttgcc catcacataa ctgactcgga aatttctaaa gagtaaaatt gcccaaaagg
     6901 ctgccataat atagcggcca gtcatccagg cttcattgaa aaaataggtc tcagcagctg
     6961 taaatagcgc cataataata aattgttgtt tattcataaa cttattgtat catgaaatct
     7021 ttctttagtc ttcctctacc ttcactttat cagccaaaaa ttcaaatcct tctggaatca
     7081 ggctttcttc tacttctttt tcagtttgag aagatttggc tttggctgaa aaggctgcgc
     7141 gaacttcttt ccattcctcc atggaaatag ctaaaatctc aggtgaaaaa cctgccgcct
     7201 gactgaggat attaccaaac atggtattga gattgtctcg tttcatagtt tgaccagcat
     7261 tgaagttaga ctcaaaagca agaatagcat ggtgttcatt ggcagcaacc ggttgagaac
     7321 caactagcag agccttgtcc ggcccaccta gactttcaat tacctctccc caggcattct
     7381 gcaaacgaat tagattttga cgtgctaaat caggattttc gacggcctct tgtaagatag
     7441 attgcacttt attgcgatcg acacgataga ctgttttgcc cgtagctggt cgactaggag
     7501 ctggtgcaac ttgtttagga accgcacctg cattagaaag ctcttgtttg agacgggcaa
     7561 cttcctgtct cagcgtagca atttcatttt caaccgctcc tgatagagct ggttcgggct
     7621 tgatttccgc caaacggacg gtcatcattt cagcataaat cttgggctgc aaactagact
     7681 taatatctgc taaattcact gttgctaagc gaatcatttc aaacagattt ttttgaggaa
     7741 gtgccaaatt ttctacaaag actgaactat gatgagtatt ttctccccct gtttgaacaa
     7801 ttaacaagtc tcttaaatag tgcaaaagat cggtcacaaa acgagtcatg ctcttaccat
     7861 tgtcaaaaag aagattcaag caagacaaag ctttgggaac atcctgttga gacaaggccg
     7921 ccacataatc atccaaggct gataggctaa tggtgccagt aatttcttca gagatagcag
     7981 tcgtcagctc atttccctgt gtcaaactca gggcttgatc caaaatagac aaggcgtccc
     8041 gcattccacc ttccgcccgt ctggcaatga tttccacagc ctctggttca gaactgatat
     8101 tttctttttc taagatatag tgaatatgtt ccttaatatc ctgtgtctta attgatttaa
     8161 actcaaaacg ttgcacacgg gatagaatag tagcaggaat cttgtgcaat tcagtagtgg
     8221 ccaaaataaa gactacattc tgtgttggtt cttccagcgt ctttaggagg gcattaaaag
     8281 cccctgtaga cagcatgtga acctcatcta tgatataaac cttataacga gcaaggctag
     8341 gcgcataggt agatttatca cgaatttcgc gaatttcatc taccccatta ttagaagctg
     8401 catccatttc aatgacatct tctaaactac cgtccgtcac tgcttgacaa atatagcagt
     8461 tattgcaagg ttcgccaccc acttgattgg gacagttcat agccttggca aagattttag
     8521 caacactggt ttttcccgtt ccacgaggac cagaaaaaag ataagcgtga cttattttct
     8581 cttgctccac cgcttgttta agagtcttag ccacaacttc ttgaccaact aactgggaga
     8641 agttttgact tctatatttt cgataaagtg cttgatacat taagattttt cctcaaacat
     8701 cgtaaagtcc catgctgtct tttcaagcaa aatagcgaca aattgttcca aataatctcg
     8761 atccatagca tcgtaatcct caatctctga agaatccaga tccagaactc caagtaactg
     8821 accattcttc atcatcggca ccacaatttc acttttagct agactatcac aagaaatata
     8881 gttgagatag gtcgtcacat ctccaacaat aacagtttcc tgaaagtgag ctgcctcacc
     8941 acaaacaccc ttgcctagtg caatacggat gcaggaaaca cctccttgga aggggcctaa
     9001 aaccaattcc tttccatcga acaaataaaa gcctgcaaat acggtattag gaaaacgtga
     9061 ttttatgaga gcactggcgt tggaaagatt agccaaaaca ttggtttcgc cttccaatag
     9121 gaaggacaat tcttcattta acatttgata acgtgattgt ttttctgatt ttaacataga
     9181 actattatat caaaaaagga gcagagacaa aagaaaaatc ccttgtttag taaacaaagg
     9241 attttgggct ctataatatt tgtagtgggt aaatccccta tggatattat ggagccta
//
LOCUS       DAVMEI010000073         9099 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVMEI010000073 DAVMEI010000000
VERSION     DAVMEI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.073"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..289
                     /locus_tag="VYQ24_001286"
     CDS             <1..289
                     /locus_tag="VYQ24_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968421.1"
                     /translation="KKDMYKEREDKFGKLNKVSFIDPNGSWARQQKVTIDNINRLEKM
                     IEDAVKFDAEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            333..5546
                     /locus_tag="VYQ24_001287"
     CDS             333..5546
                     /locus_tag="VYQ24_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1968422.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNMDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            5818..6669
                     /gene="ylqF"
                     /locus_tag="VYQ24_001288"
     CDS             5818..6669
                     /gene="ylqF"
                     /locus_tag="VYQ24_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1968423.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6656..7435
                     /locus_tag="VYQ24_001289"
     CDS             6656..7435
                     /locus_tag="VYQ24_001289"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1968424.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7451..9001
                     /locus_tag="VYQ24_001290"
     CDS             7451..9001
                     /locus_tag="VYQ24_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1968425.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNILITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3049 a         1542 c         1888 g         2620 t
ORIGIN      
        1 caagaaagat atgtataaag aaagagaaga taaatttgga aagttaaata aagttagttt
       61 tatcgatcca aacggttctt gggctcgaca acagaaagtg actattgata atattaatag
      121 acttgaaaaa atgattgaag atgcagtaaa atttgatgct gaggatgaag ttgccaaatt
      181 atatcctgag acaaatagta gagttcttaa actgaaaaaa gcaatcttta aagcttatct
      241 tgaccaaacc ggtgatttta gaagttcaat ttttgagaat aaaaaatagt gtttactatt
      301 aggaaataaa aagtgaaggt ggaggattag aattgtcatt attaaaaaaa gataaatttt
      361 ccattcggaa aataaagggt attgttggtt cggtattctt ggggagtctt ttatttgcac
      421 cgtcagttgt tggtgcatcg acttatcatt acttagatta tagtagtttg acacaaactg
      481 aacgtgatca actcaaacaa ggtagacctg atgaatccaa agagtcatat gctttggatt
      541 atgaaaaaga cgcactacca aacacaggaa gttctcaatc tattatgact gcattgggtt
      601 tgttagctat cgggagtctt attgtgatta tcacaaaaga taatagaaat aaaaaaatag
      661 ctacattttt gattgtaggg gcgacaggtt tagttacact atcaactgct tcagcactta
      721 atttgaatgc taacatccat gagtctggac gtgatggtgt gttgcaaatt tctggttaca
      781 gatatgttgg ttacttggag cttgatgata aaactgtttc gtcggtttct ccagcatcaa
      841 ctgtttcgcc agttgagcaa cccaaagttg taacagagaa aggcgaaccg gaagttcatg
      901 agaagcctga ctatacgcaa ccaataggtg caaaccttgt tgagcctgaa gttcatgaga
      961 aactagcata cactgaacca gtagggacaa caggagtaga tgaaaatgga aacttgattg
     1021 aaccacctgt taacgacatc ccagaatata ctgagccagt aggtacaaca ggagtggatg
     1081 aaaatggaaa cttgattgag ccacctgtta gcgacattcc agagtatact gaaccaatat
     1141 ctacagtttc agaagttgca tcagaaagag aagagttgcc ttctttacat accgatattc
     1201 ggacagagac tattcctaaa accactatag aagaatctga tcctagcaaa tttataggcg
     1261 atgattctgt tagacaagtt ggtgaggatg gcgaacgtca aattgttact agctatgaag
     1321 agttacatgg caaaaaaatc agtgacccag ttgaaacagt aactattttg aaagaaatga
     1381 agcctaaaat tcttgtaaaa ggtaccaaag aaaaacctaa agaaaaaacg gctcctgttt
     1441 tgaccttaga tagaacaaat acaaatgtat taaaccgatc cgcaactctt tcttatcatt
     1501 tagttaatac agatggagtc acaatcaata aaattactgc gacaattaaa gatggaaatg
     1561 aaattgtcaa aacagtagat ttgacttcag agcaattaga taagcaggta gaagatttaa
     1621 aattctacaa ggactataag atagagacca ctatgaccta tgaccgtggc aagggagaag
     1681 aaactgcaac attagaagag aaaccacttc gcttggattt gaaaaaagtt gaaattaaaa
     1741 atatcgcatc tactaattta gtgaaggtaa atgacgatgg tactgaaaca cctagtgatt
     1801 ttatgactga aaaaccttca gatgaagatg tgaagaaaat gtacttaaaa attactagtc
     1861 gtgataataa agtaacacgt ttagcagttg ataaaattga agaagtgact gaagaaggta
     1921 agaaacttta taaaatcact gccgaagctc aagatttgat tcaacataca gacccaacga
     1981 aggttcgtaa caagtatgtt cattatattg aaaaaccagt tccaaaagtt gatgacgttt
     2041 attataactt taaagagtta gtagatgcca tgaatgctga taaaaatggt acatttaaaa
     2101 tcggagcaga tttgaatgca acgaatgtac caacacctaa caaacaatat gttccaggta
     2161 cgtttaaggg acatctatca agtgttgatg gtaaacaata tacaattcat aacattgccc
     2221 gtcccttatt tgaccgtgtt gaaaatggtt ctgtgaaaaa tattaattta ggtaatatgg
     2281 atatcaatat gccttgggct gacggaatag cacctgttgc caatatggtg aaaaatgcaa
     2341 ctgttgaaga tgttaaggta actggtaatg tagttgctaa taacaatatt gcgggtatcg
     2401 tcaataaaat cgatagtggt ggtcagttga caaatgtagc cttcatcggt aatcttacag
     2461 gtgttggtga taaaggtcaa tatatggctg gcattgccgg tgagatttgg agaggtaatc
     2521 ttgcaaaagc atatgtcgag gctgatattg ttgctaacag agcccgtata ggtggtttag
     2581 ttgctaaaac agataatggt aacgactcta tgggaattgg taaatacggt tcaattcgta
     2641 aatcagtcac caaaggaacc attaagacta aagttttgtt tgaaacaggt ggatttatca
     2701 atagcaatct tccttttggt aaacttgaag ataatatttc aatgatgaga gttgaaaatg
     2761 gtgaagagtt ctttggttct tccgatttgg actacgatgg tggttatttc acaaatggct
     2821 ggttagagcg taactttgtt gtcaaaggtg tcagttcagg taagcattca tacaaacgtt
     2881 ctcgtgacaa aattaaagaa attagtcaag atgaagctaa taagagaatt gcaaattttg
     2941 gacttactgc tgataaatac gaaatcaacg aacctgtggt caatcgctta aatcgtttaa
     3001 ccagaagaga agatgaatat aagtcaactc aagactacaa atccgaacgt gatttagctt
     3061 atcgtaatat tgagaaactt caaccatttt ataataaaga atggattgtc aaccaaggta
     3121 acaaacttgc agaagattca aacttagcta agaaagaagt cctttctgtt acggggatga
     3181 aggatggtca attcgtgacc gatttatcag atattgataa gattatggtt cactatgctg
     3241 atggtactaa ggaagaaatg gatgttacta agaatactga ttctaaagtc caacaagtgc
     3301 gtgaatatag tgtatctggt cttggtgatg ttgtttatac accgaatatg gttgttaaaa
     3361 atcgtgataa attgattgct gatgttaaat ctcaattatc aagtgtggaa ttgatttcac
     3421 aagaagtaag agatttaatg agtagacgtg ataaacctgc agagaatacg gatgaacgta
     3481 agaatggtta tattaaagat ctatacttag aagaaagctt tgccgaagtg aagcaaaatc
     3541 tagataagtt agtcaaatcc ttggtcgaaa atgaagacca tcaattgaat ggtgatgaag
     3601 ctgcgataaa atcacttctc aagaaagtgg aaaccaataa agctaaaatc atgatggctc
     3661 tgacttatct caatcgttac tatgatatca aatacggtga cataagtata aaaaacatca
     3721 tgatgttcaa acctgatttt tatggtaaaa caccaagtgt tattgacaga ctcatcaaca
     3781 tcggttcaag tgaaaagaac ttgaaagggg atcggactca ggatgcttac cgtgaaatca
     3841 tcgcaggtaa tactggtaaa agtaatttgc gtaacttctt agaatacaac atgcgtctgt
     3901 ttacagagga taaagacatc aatgattggt ttatccactc agctaagaat gtttatgttt
     3961 cagaacctaa gacaacaaat acagagttga aagataaacg tcatcgtgtg ttcgatggtt
     4021 tagataacgg tgttcatggt cgtatgattt taccacttct aacgcttaaa gatgcccata
     4081 tgttcttaat ttcaacatat aacacgatgg cgtacagttc atttgagaaa tatggaaaac
     4141 atacagaaga agctcgtaat gagttcaaaa ctaagattga tgaagttgca catgctcaac
     4201 aaacttattt agatttctgg tccagattag ctctgcctaa tgttcgtgat agacttctta
     4261 agagtcaaaa tatggtaccg acaccagttt gggataacca gacctacaat ggttctcctg
     4321 taggtcgtcg aggatttgac agtaaaggta accctatagc tccaattcgt gaattatatg
     4381 gtccaacatg gcgacaccat gatagagact ggcgtatggg cgctatggca tcaatattcc
     4441 ctaatccaaa taatgatgac aaggtcttgt ttatggtgac ggatatgatt agcccatttg
     4501 gtatttcagc ctttactcac gaaacaacac acgtaaacga tagaatgctt tacttcggtg
     4561 gtcataagca tcgtcaaggt acagacgttg aagcctatgc tcaaggtatg ttgcaaacac
     4621 ctgacagctc aacgactaat ggtgaatacg gcgcacttgg aattaacatg gcttaccatc
     4681 gtccaaatga tggtaaccaa tggtacaacc cagatcctga caaattaaag acacgtgatg
     4741 acattgaccg ctacatgaga aactacaatg aagctatgat gttgcttgat catgtagaag
     4801 ctgatgctgt acttcctaaa attaaaggag acaatagtaa gtggttcaag aagattgaca
     4861 aagaaatgcg ctctaagatt cagtataatg atcttttagg tccaaaccaa tgggatagta
     4921 tccgtgattt gaaagatgaa gaaaaggtaa tgactttatc tagcgtcaat gatttagtgg
     4981 ataataactt catgacaaaa catggaaatc caggtaatgg acgttataga ccagaagatt
     5041 ttacaccaaa ctcagcttac gtaaatgtca atatgatggc aggaatttac ggtgggaata
     5101 cgagtcaagg tgctcctggt tcattatcat ttaagcacaa tgctttccgt atgtggggct
     5161 attatggtta tgaaaatggc tttattagtt atgtatcaaa taaatacaaa gcagaagcag
     5221 ataaaaacaa ccacggttta ctaagcgata agctaataat taataaagta tcgaaaggaa
     5281 actttaatac tcttgaagag tggaagagac attggtacgg ggaagttctt gctaaagcta
     5341 agaaaggttt tgaggctatc gatattgatg gcgtccatat cagtaactat gatgagttaa
     5401 ggcctttatt tgataaagca gttgaagaag atttgaagaa accagatgac ttctctcaca
     5461 ctgttgctct taaatctaag gtcttcaaag cccttcttaa aaacacagac ggtttcttta
     5521 ataaactgtt taaagaagac atttaaatgt ttagaatata gaaggaacag ttttttgctc
     5581 cctctgaaaa gtcatcattt gatggctttt ttctatatag ggtaaaagat aggctaaaag
     5641 atagggtaaa aggctatcat cggacaaaat aaagaaggca tgatataata taaagtagat
     5701 ttctatgtca taaaacaaga actgtttgga catcattcat ttgaaaactc tctatgttca
     5761 aactatagta aaataaaata ggggatctaa atccttgcta cgaaaggaaa aaactcaatg
     5821 gctactattc aatggtttcc tggtcacatg tctaaagcgc gtcgacaggt gcaggaaaat
     5881 ttaaaatttg ttgattttgt gacgatttta gtagatgcac gcttgcctct atctagtcaa
     5941 aatcctatgt tgaccaagat tgttggtgat aaaccaaaac tcttgatttt aaacaaggcc
     6001 gacttggctg atccagcaat gaccaaggaa tggcgtcagt attttgaatc acaaggaatc
     6061 cagacgctag ctatcaactc caaagagcaa gtgactgtaa aagttgtaac agatgcggcc
     6121 aagaagctca tggctgataa gattgctcgc cagaaagaac gtgggattca gattgaaacc
     6181 ttgcgtacca tgattattgg gattccaaac gctggtaaat caactctgat gaaccgtttg
     6241 gctggtaaaa agattgctgt tgttggaaac aagccagggg tcacaaaagg tcaacaatgg
     6301 cttaaaacca ataaagatct ggaaatcttg gatacaccgg ggattctctg gcctaagttt
     6361 gaggatgaaa ctgttgcact taagttggca ttgactggag ctatcaagga tcagttgctt
     6421 cctatggatg aggttaccat ttttggtatc aattatttca aagaacatta tccagaaaag
     6481 ctggctgaac gcttcaaaca aatgaaaatt gaagaagaag cgcctgtgat tattatggat
     6541 atgacccgcg ccctcggttt ccgtgatgac tatgaccgtt tttacagtct cttcgtgaag
     6601 gaagtccgtg atggcaaact cggtaactat accttagata cattggaaga cctcgatggc
     6661 aacgattaaa gaaatcaaag aactccttgt gacagtcaag gagttagaaa gccctatttt
     6721 tttagagctt gaaaaggata atcgctcagg agttcaaaag gaaatcagca agcgtaaaag
     6781 agccattcaa gctgaattag atgaaaattt gcgcttggaa tccatgcttt cttatgaaaa
     6841 agaactttat aagcaaggat tgaccttaat tgcaggtatt gatgaggttg gtcgtggtcc
     6901 tcttgctggt cctgtagtcg ctgcggccgt tattttacct aaaaattgta agattaaagg
     6961 tctcaacgac agcaagaaaa ttcctaaaaa gaaacatctg gagattttcc aagccgttca
     7021 agaccaagcc ttgtcgattg gaattggtat catagataat caggtcatcg accaagtcaa
     7081 catctatgaa gcaaccaaac tagccatgca agaagcaatc tcccagctca gccctcaacc
     7141 agagcacctt ttgattgatg ccatgaaact ggacttgccc atttcacaaa cctccattat
     7201 caaaggagat gccaactccc tctctatcgc agcagcatct atagtagcca aggtaacacg
     7261 tgatgaattg atgaaagaat acgatcagca gttctctggc tatgatttcg ctactaatgc
     7321 aggatatggc acagctaaac atctggaagg cctcacaaaa ctaggagtta ccccaattca
     7381 ccgaaccagc tttgaacccg ttaaatcact ggttttaggt aaaaaagaaa gttaattgaa
     7441 aggaaatacc atggaggaac agtcggaaat agtccgttct aagaaagaat tcgcctttgc
     7501 atccagcact atactatccc aagttggtcg aggaatcatt gtcggcctca tcgttggaat
     7561 tatcgtcgga tcctttcgtt tcttaattga aaagggcttc cacctgatac aaggagttta
     7621 tcaagatcaa gggtacttag tgcgcaatct ttttgtactg gttttgtttt atatactcat
     7681 ctgttggctc agtgccaaac taacacggtc agaaaaagat attaaaggct caggaattcc
     7741 tcaagtcgaa gccgaactga aaggcctcat gtccctcaac tggtggggca ttctttggaa
     7801 aaaatatgtg ctaggtattc ttgctattgc cagtggactc atgctgggtc gagagggacc
     7861 cagcattcaa cttggagcag ttggtggtaa aggaattgcc aagtggctca aatccagtcc
     7921 agtagaggaa cgttccttga ttgccagtgg agctgcagca ggtttagccg cagcctttaa
     7981 tgctcctatt gcagcacttc tctttgttgt agaagaagtc tatcaccatt tttcgcgctt
     8041 tttctgggtc tcaactctag cagccagcat cgtagcaaac tttgtgtctc tactcatgtt
     8101 cggtttgaca ccagtattgg atatgccaga taacattcct cccatgaccc tagaccagta
     8161 ttggatatat ctcgtcatgg gaattttcct tggattttca ggttttctct atgagaaagc
     8221 tgtattaaac gttggaagag tttatgactt gattggtcaa aaaatccatt tggatagggc
     8281 ttattatccc atcttggctt ttatccttat cataccagtc ggaatcttct tacctcaaat
     8341 cattggtggc ggaaatcagc ttgtcctttc tttaactgaa caaaatttta gtttccaagt
     8401 tttattagct tactttttaa tccgctttat ttggagtatg attagctatg gaagtggact
     8461 gccaggagga attttcctcc ccattttagc tcttggttct ttgcttggtg ccttagttgg
     8521 tgttatctgt gtcaatcttg gacttgtcag tcaagagcaa ttccctatat ttgtcattct
     8581 aggaatgagt ggctattttg gagccatatc aaaagctccc ttaaccgcta tgatcctcgt
     8641 aactgagatg gtaggagata ttcgcaacct tatgccactt ggtcttgtca ctcttgtttc
     8701 ttatattatc atggatttgc tcaaaggtac gccagtctat gaagccatgc tggaaaaaat
     8761 gcttccagaa gaagtatcta gcgaaggaga agttacactt atcgaaatac cagtttctga
     8821 taaaattgct gggaaacaag ttcatgaact caacttacca cacaacatcc tcatcacaac
     8881 tcaagtccat aatggcaaga gccaaacagt taacggctca accagaatgt atctgggtga
     8941 tatgattcac ctggttattc caaaaagtga aattggaaaa gtcaaagatt tgttgttgta
     9001 gtatgatcat ttacataatt tatgttatgt aaatgatcgg tttgatttat ttagaaaacc
     9061 gattctcaga aatgagatcg gttatttttt actgatgag
//
LOCUS       DAVMEI010000074         9094 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVMEI010000074 DAVMEI010000000
VERSION     DAVMEI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.074"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            86..406
                     /locus_tag="VYQ24_001291"
     CDS             86..406
                     /locus_tag="VYQ24_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1968426.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            410..1225
                     /locus_tag="VYQ24_001292"
     CDS             410..1225
                     /locus_tag="VYQ24_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968427.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSIFTISLVIGIWNWKEAVFRPFIS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            1300..2226
                     /locus_tag="VYQ24_001293"
     CDS             1300..2226
                     /locus_tag="VYQ24_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1968428.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDVLMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            2292..2558
                     /locus_tag="VYQ24_001294"
     CDS             2292..2558
                     /locus_tag="VYQ24_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1968429.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            2551..3249
                     /locus_tag="VYQ24_001295"
     CDS             2551..3249
                     /locus_tag="VYQ24_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1968430.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKSLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            3260..3739
                     /locus_tag="VYQ24_001296"
     CDS             3260..3739
                     /locus_tag="VYQ24_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1968431.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQYRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            3747..4475
                     /locus_tag="VYQ24_001297"
     CDS             3747..4475
                     /locus_tag="VYQ24_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968432.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            4517..5386
                     /locus_tag="VYQ24_001298"
     CDS             4517..5386
                     /locus_tag="VYQ24_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1968433.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLDKEVWYRFSELTNTEANCLAGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            5361..5801
                     /locus_tag="VYQ24_001299"
     CDS             5361..5801
                     /locus_tag="VYQ24_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1968434.1"
                     /translation="MIPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLGDLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            5924..8470
                     /locus_tag="VYQ24_001300"
     CDS             5924..8470
                     /locus_tag="VYQ24_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1968435.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(8528..>9094)
                     /locus_tag="VYQ24_001301"
     CDS             complement(8528..>9094)
                     /locus_tag="VYQ24_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV1968436.1"
                     /translation="LDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQ
                     DSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFF
                     GLIEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSRA"
BASE COUNT         2732 a         1534 c         2062 g         2766 t
ORIGIN      
        1 tatgctttac aaactagtgt agaagtaata tactataggt gaagttacta gtaggtatta
       61 caaaatagat tagaagggag aagtgatgaa agaaactcag ctattaaaag gtgttcttga
      121 aggttgtgtc ttggatatga ttggtcaaaa agagcggtat ggttatgagt tggttcagac
      181 tttgcgagag gctggatttg atactatcgt tccaggaact atttatcctt tgttgcaaaa
      241 gttagaaaaa aatcaatgga taagaggcga catgcgcccg tcgccagatg gtccagatcg
      301 gaagtatttt tcattaatga aagaaggaga agagcgtgtc tcagtctttt ggcaacaatg
      361 ggacgatttg agtcaaaaag tagaagggat taagaatggg ggttaaacca tgaagaaaat
      421 gaagtattac gaagaaacaa gcgctttgct acatgagttt tctgaggaga atcaaaagta
      481 ttttgaggag ttgtgggaaa gttttaatct tgctggattt ctctatgatg aagactatct
      541 cagagagcag atctatttga tgatgctaga tttctcagaa gcagaacgag atggcatgag
      601 tgcagaggat tatctaggta agaatcctaa aaaaataatg aaagagattc tcaagggagc
      661 acctcgcagt tctatcaaag agtccctttt gacgccaatt cttgtcctgg cggtattacg
      721 ttattatcaa ctactaagtg atttttctaa aggtcctctc ttaacagtca atttgctcac
      781 atttttaggg caacttctta tttttctgat tggatttgga cttgtggcca caattttacg
      841 aagaagttta gtccaagatt ctcctaaaat gaaaattggc acttacattg ttgttgggac
      901 tatagttctt ctagttgttt taggatatgt aggaatggca agcttcatac aagaaggagc
      961 cttttatatt ccggctccct gggatagttt gtctatcttt acgatttcgc tagttatcgg
     1021 tatttggaat tggaaagaag cggtctttcg tccatttatc agtatgatta ttgcccatct
     1081 tgtggtgggt tctctgctcc gttattatga gtggatggga atttcaaatg ttttccttac
     1141 aaaagttatt cctttagctg tcctctttat tggaatcttt gtcttgttcc gtgggtttaa
     1201 gaagataaaa tggagtgaag tatagtcaaa aagtcgttgc aaagcggttt tttgttataa
     1261 tgaagggaag aatgttagaa attaaaggag aaaaatttga tgagatacat aactcttggt
     1321 caagatgaca aagaattatc agaaattgtt ctcggaatga tgagaataaa agataaatct
     1381 gtaaaagaag ttgaagagct tgtagaaaca gcactttctg ttggaatcaa tgccttcgac
     1441 ttggctgata tttatggtcg tggtcgttgt gaagaactgt taggtcttgt cctaaaaaat
     1501 cgtccagatt taagagaaaa gatgtggatt cagtccaaat gtggcattcg cattgaagaa
     1561 tttacctatt ttgatttttc taaggactat attataaaat cagtagacgg tattttgcaa
     1621 agattgaaga ttgatcatct agatagcttg ctccttcatc gaccagatgt tttgatggaa
     1681 tctgaccaag tagcagaagc ctttaatctc ctttataaac aaggtaaagt tcgagatttt
     1741 ggagtttcta atcaaaatcc tatgatgatg gagttgctta aaaaagatgt caagcagccg
     1801 ttagctgtta atcagctaca attgagtgcg gcttttactc caggatttga atcagctttt
     1861 catgttaata tggaagatag tcaagcagct atgcgagatg gcagcatttt tgaatattgc
     1921 caattacacg atgtggtcat tcaagcatgg tctgtcttac aattcgggta ttttaaaggg
     1981 aattttgttg gaaatgagaa atttcaagct ttaaatcaag tacttgatcg tttagctatt
     2041 aaatatggag taacctcttc aactattgcc atttcttgga tattgcgtta tccagcaaaa
     2101 atgcaggcag tcgtaggtac aacaaatcct aagcacttga gagaagttag ccgagcggca
     2161 aactttagct taacaagaaa agaatggtat gaaatttatc ttgctgcagg gaataatttg
     2221 ccgtaagtag tagcaggttt gaataactca cattcaaaaa gccgtataaa gcggtttttt
     2281 gctataatta aatgaagaat gtagaattaa aggagaaaaa catgacattt gaagagatcc
     2341 tgcctgggtt aaaggctaag agaaaatatg tacgaactgg ttggggaggt gcggaaaact
     2401 atgtccaact ctttgatacc atcgagcaaa atgggcttgc acttgaaatg acaccttatt
     2461 tcctaatcaa cgtttctggc gaaggagaag gtttttccat gtggagcccg acagtttgtg
     2521 atgttttggc aacggattgg gtagaagtgc atgactaaac gtgtactcat tacaggagtg
     2581 agttcaggga tcggattggc tcaagctcgc ctctttttag agaagggcta tcaagtttat
     2641 ggagttgacc aaggtgaaaa gtcactctta gagggtgatt ttcacttttt acagagagat
     2701 ttgaccttgg acttggaacc tatttttgac tggtgccctc aggtggatgt tttgtgtaat
     2761 actgctggag ttttggatga ttacaaacca ctgttggaac aaacggcgca ggacattcaa
     2821 gagatttttg aaatcaacta cattattcca gtagagttga ctcggtatta tttgacacaa
     2881 atgctggaaa ataaaaaggg aatcatcatc aatatgtgtt ccattgcttc tagcctagca
     2941 ggtggaggtg gtcacgccta tacttcatcc aagcatgcct tggctggctt caccaagcag
     3001 ttggctctag actatgctga agctgggatt caggtctttg gtatcgctcc aggagcagtc
     3061 aagacagcta tgaccgctgc ggattttgaa ccaggtggtt tggctgactg ggtggctagt
     3121 gaaaccccaa tcaagcgctg gattgagcca gaggaaatag cagagcttag tcttttttta
     3181 gcaagtggaa aagcgagcgc catgcaagga caaatcttga caatagatgg tggctggtct
     3241 ttgaagtagg aggagttgga tggaaatcaa aaaacacttt ggagtctacg ctgtttgctt
     3301 agaaaatggg aagttactct gcattgaaaa aacgagaggc ccttatcaat atcggtatga
     3361 tctacctgga ggcagtcagc aacttggtga aggactgacg gaaacgctga ctagagaagt
     3421 tatggaagag acgggattta ctgttagaag ctactctaat cctcgaatct acgatgtttt
     3481 cgttagggaa gagttaaaaa attttatggt tcaccatgtc atggccttgt atgatgttga
     3541 aatgaatgag agtgcacctc aagttacgac ttcggaagct gtgtctgatg gtgcgaatga
     3601 ttcacttgga tatatttgga tggatattca agagattaca gaagaaaatg catcgccatt
     3661 agtcttgaag gttaagtctg aattattagg atttccagaa ctggacaaga cttcttacat
     3721 gaattggaag gtgaagtaga gggataatgg aactatttaa aacatggaag aaaaatatgg
     3781 ttctctatgg tcttaaatct caaattggaa ctgtctaccg aaacaatgat aggacaacaa
     3841 gcttttatga tgttgggaat tttctatacc tagcagggga gttggattcc agattttggg
     3901 aagattttgt tagaaaatat ggtttagatt ataagattat tatttcagaa aatactaatt
     3961 ggcaagattt tctgcatcga aaagtggggc taaattcttt tactcgctat tcttttaaag
     4021 ataaggcaaa ttttcaagtt gaatttttaa ataatctagt tactcattta gaggaaggtt
     4081 acaatattgt gcctattgat aatcatattt ataactgctt ttctacggaa gaatggtcac
     4141 aggatttaca gggggatttt gagtcctatc aggattttgt tttaaaaggt ggatttggct
     4201 ttgtgattct taaaaataat gaactgattg ctgggatttc ctcagggtta gtttaccgta
     4261 aagcagttga agtggaagtt gcaactagac caaacgaaca aggaaatgga tttgctaaaa
     4321 agcttggtgc tgcaatgatt ctagagagtt taaatagaga tatgtttcca ctttgggatg
     4381 ctcataatga ggcttccaaa aaagtagcag aatttttagg atatgagtta tctgaacctt
     4441 atgaagcttt tgaactagag gaaattttga tataattata tatgatattg tatcttttaa
     4501 gacgaggtga atggctatga gaaatcaact tgcccttagt ggcgaaaaaa ttcttgaaaa
     4561 aatttatcct caactatttc accacgtcgg catgattcgt ggggaatact tattgagaga
     4621 gcttaatcaa aacatcatat tagcaagttg tcagcaattt gtaaaagatt atcttgatac
     4681 tatttgctcc ttttacttag ataaggaagt ttggtatcgt ttttctgaat taacgaatac
     4741 agaagccaat tgtttagcgg ggactagaga gttttttgac gaagggcatc ccttgtttgg
     4801 ctatagagga accaggcgtt tactggcgtg tttggatgaa tttcaggctg aagcacatgt
     4861 cgttacagaa gtttatcaaa ccaatcccaa tctgtctctt atttttcctt ttgtcaatga
     4921 tgccgaccaa ttaaaacaag ctattacagt attgcgtcag tatggcttta ctgggaaagt
     4981 cggaacgatg attgaattac cgtcagcgta ttttgactta tctagtatac tggaaacggg
     5041 catttcaaag attgtagttg gaatgaatga tttgacttcc tttgtttttg cgactatgag
     5101 aaacagtcaa tggcatgata tggaaagtcc aataatgtta gatatgctaa gagatatgca
     5161 ggataaagca agaaagaaca agattaattt tgctgtagca ggctatctga atacttcttt
     5221 catacaaaaa atgaatcagt tgggtatcaa gtgcattatc cactacagtt ctattccaga
     5281 gatttttgat ttagagattg accatccaga tcatcttaaa cacataaaag aagaaagtaa
     5341 aaaattacaa aggagtaccc atgataccgc aagaaatgtg gaataaatac aagcaaatca
     5401 atccttctat cggagatgaa atcgatgctt gggcttttgg agttgaacca gaccttttag
     5461 gggatttggt gtttaaagga gaaaagacag caacagcatc agcctatgac ctctatgtac
     5521 tagaagacga accacttccc caagtaggga ccttcgatgt cattttagat agtcaaaatc
     5581 aggctgtctg cattgtcgaa attacaaagg tttctgttga actcttcaat caagtttctg
     5641 cgcaacatgc ctttaaggaa ggtgagggag acaaatcact tgcctattgg cgccaggttc
     5701 atgaggactt tttcacagac tgtttgggtg aagtagggct gacttttaca cctgaaagca
     5761 aggttgtttt agaagaattt cgcaaggtct acccactgta gactattaga aggaagaaag
     5821 ttttggaaat cgctgtccaa tccttttttc tcaagcaaaa tatgatataa taagtttgtt
     5881 tgaagaagag cagcagctct taaacttaga ataggagaaa actatgcaag cagttgaaca
     5941 ttttattaag caatttgttc ctgaacatta tgatttattt ttagatttga gtcgtgagac
     6001 caagactttt tctgggaaag tgaccatcac tggtcaagca cagagtgacc gcatctccct
     6061 ccaccaaaaa gacttggaaa tcacctctgt agaagttgca ggtcaagctc gtccatttac
     6121 agttgaccat gacaatgaag cccttcatat cgaattggct gaggctggtc aagttgaatt
     6181 ggttcttgcc ttttctggta aaattacaga caacatgaca gggatttacc cttcttatta
     6241 tacagttgat ggagtcaaga aggaggtctt gtctactcag ttcgagagcc attttgcgcg
     6301 cgaagctttc ccatgtgtgg atgagcctga agccaaagca acttttgacc tctctcttcg
     6361 ctttgaccaa gcagaaggtg aattggcctt gtcaaacatg ccagaaatcg atgttgaaaa
     6421 ccgtaaggaa acaggtatct ggaagtttga gacaacacct cgcatgtctt cttacttgtt
     6481 ggcctttgtt gctggtgatt tgcaaggggt gaccgctaaa actaaaaatg gtaccctggt
     6541 aggtgtctac tcaaccaaag cacatccact ttcaaatctt gatttctcac tggatatcgc
     6601 tgttcgctct atcgagtttt acgaagatta ctatggagtt aagtacccaa ttcctcaatc
     6661 tctccacatc gcccttcctg acttctcagc tggtgctatg gaaaactggg gtcttgtgac
     6721 ctaccgtgaa gtttacttgg ttgtcgatga gaactctaca tttgctagcc gtcaacaagt
     6781 tgcccttgtt gtggcccatg aattggctca ccaatggttt gggaacctcg tgactatgaa
     6841 atggtgggat gacctttggc tcaatgaaag tttcgctaat atgatggaat acgtctgtgt
     6901 ggataccatc gaaccaagct ggaatatctt tgaagatttc caaacaggtg gagtacctct
     6961 tgctcttgaa cgtgacgcta ctgatggcgt tcagtctgtc cacgtcgaag ttaaacatcc
     7021 agatgaaatc aatacactct ttgacggcgc tatcgtctat gccaaaggaa gccgtctcat
     7081 gcacatgctt cgccgttggc taggagatgc tgattttgct aaaggtttgc atgcctactt
     7141 tgaaaaacac caatacagca acaccattgg tagtgacctt tgggatgccc ttggtcaagc
     7201 gtcaggacgt gatgtcgcag ccttcatgga ttcttggttg gaacagcctg gttacccagt
     7261 tcttactgtc aaagttgaaa atgatgtctt gaagatttca caaaaacaat tcttcatcgg
     7321 tgagaacgaa gacaagaacc gtctctgggt ggtgcccctc aatagcaact ggaaaggctt
     7381 gcctgataca ctcgaaactg aaagtatcga aatccctggc tacgcagctc ttcttgctga
     7441 aaatgaagga gctcttcgtc tcaacactga aaatactgcc cactatatta ccgactatca
     7501 aggagacttg ttagaagctg ttcttgctga gctagagaca cttgataaca caagcaaact
     7561 gcaaattgtt caagaacgtc gtttgttggc tgaggcaggg cacatttctt atgcagactt
     7621 gcttccagtt cttgataaac ttgctaagga agagtcttat ctggtggttt cagctgtttc
     7681 tcaagtgatt tcagcccttg agcgctttat tgatgaagga acggatgctg aaacagcctt
     7741 taaaggactg gttgctaaat tggctcgtca taactatgac cgtcttggtt ttgaagctaa
     7801 agacggagaa tcagatgagg atgaattggt tcgtcagctg gctgtttcga tgatgattcg
     7861 ctcaaatgat gcagaagcta gtcaagtcgc tagccaaatc ttcgcaacac acaaggagaa
     7921 tcttgcagga ctcccagcag ctattcgctc acaagttctg attaatgaga tgaaacatca
     7981 tgagactaaa gacttgttag cactttatct tgatacttat actcacgcaa cagatgctgt
     8041 ctttaaacgc cagttggcag cagctcttgc atacagtaca gatgcggata atatccaaaa
     8101 cctgattact tcttggaagg acaaatttgt ggtcaaacca caggacttgt ctgcttggta
     8161 ttaccaattc ctagctcatc aagcaactca gaaaacagct tggtcttggg cgcgtgaaaa
     8221 ctgggcttgg attaaggcag cccttggagg agatatgagc tttgatagct ttgttatcct
     8281 tcctgctcat gtatttaaga ctcagcaacg cttggcggaa tacaaggagt tctttgagcc
     8341 gcagctttct gaccttgctc ttagccgtaa catcggtatg ggaatcaagg aaattgcagc
     8401 acgtgttgac ttgattagcc gtgaaaaagc tgcagtcgaa gcagttgttc ttcaatacgg
     8461 aaatgcataa ataagcctaa aataaaaaga aaaggctctt tgtcaactgt agtgggttga
     8521 aaaaaagcta agctcgagaa aggacaaatt ttgtcctttc ttttttgata ttcagagcga
     8581 taaaaatccg ttttttgaag ttttcaaagt ttcgaaaacc aaaggcattg cgtttgataa
     8641 gtttgatgag attattggtc gcttccaatt tggcgtttga atagggtagt tgaagggcgt
     8701 tgacgatttt ctctttgttc tttagaaagg ttttaaagac agtctgaaaa ataggatgaa
     8761 cctgcttcag attgtcctca atgagtccga aaaatttctc agggtctttg ttctgaaagt
     8821 gaaaaagtaa gagttgatag atctgatagt ggtgtttcaa gtcttctgaa tagcttaaaa
     8881 tcttgtcaag aatttcttta tttgttaagt gcatgcgaaa agtagggcga taaaaacgtt
     8941 tatcgctgag tttacgacta tcctgttgga tgagtttcca gtaacgcttg atagccttgt
     9001 attcatgaga ttttcgttca aactgattca taatttgaac acgaaaacga ctcatggcac
     9061 ggctgagatg ttggataata tggaaacgat ctag
//
LOCUS       DAVMEI010000075         9065 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVMEI010000075 DAVMEI010000000
VERSION     DAVMEI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9065
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.075"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..183
                     /locus_tag="VYQ24_001302"
     CDS             <1..183
                     /locus_tag="VYQ24_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968437.1"
                     /translation="TFLIELFTSYFNSLYFPLYSMKIKAQTRKLATGCSKHCFEVVDK
                     TDEVSSKYCFEVADGS"
     gene            complement(367..1215)
                     /gene="dprA"
                     /locus_tag="VYQ24_001303"
     CDS             complement(367..1215)
                     /gene="dprA"
                     /locus_tag="VYQ24_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1968438.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1311..2000)
                     /locus_tag="VYQ24_001304"
     CDS             complement(1311..2000)
                     /locus_tag="VYQ24_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1968439.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2012..2890)
                     /locus_tag="VYQ24_001305"
     CDS             complement(2012..2890)
                     /locus_tag="VYQ24_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1968440.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2880..3749)
                     /gene="licA"
                     /locus_tag="VYQ24_001306"
     CDS             complement(2880..3749)
                     /gene="licA"
                     /locus_tag="VYQ24_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1968441.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(3766..4788)
                     /locus_tag="VYQ24_001307"
     CDS             complement(3766..4788)
                     /locus_tag="VYQ24_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1968442.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4793..5500)
                     /locus_tag="VYQ24_001308"
     CDS             complement(4793..5500)
                     /locus_tag="VYQ24_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1968443.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5836..7323
                     /gene="tacF"
                     /locus_tag="VYQ24_001309"
     CDS             5836..7323
                     /gene="tacF"
                     /locus_tag="VYQ24_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1968444.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIIKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7333..8136
                     /locus_tag="VYQ24_001310"
     CDS             7333..8136
                     /locus_tag="VYQ24_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1968445.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYELLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKI
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8138..8947
                     /locus_tag="VYQ24_001311"
     CDS             8138..8947
                     /locus_tag="VYQ24_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1968446.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2850 a         1827 c         1525 g         2863 t
ORIGIN      
        1 actttcctga tagagttgtt cacatcttat ttcaattcac tatactttcc cttatactca
       61 atgaaaatca aagcgcaaac taggaagcta gccacaggtt gctcaaagca ctgctttgag
      121 gttgtagata agactgacga agtcagctca aaatactgtt ttgaggttgc agatggaagc
      181 tgacgtagtt tgaagagatt ttcgaagagt attatatgag aaaaatcctc tactctacta
      241 atccacttag aaaaaacgca tattatcaag tgttaaaaac ttgctaatat gcgttttatt
      301 atggaaatgt tgggttcatt ttctcttaaa attgattttt tcttagaatt ttagcttagg
      361 tcatttttaa aattcaaatt ccgcaagaac atcttgccca ctggtgacca attttgctcc
      421 ttcttgaatc aaatgatggc aaccgtctga tagtccatct aaaatgctac caggaatagc
      481 aaagacatcg cgtccttctt ccattgctcg ctcacacgta atgagactac ctgaacgcat
      541 cttagcctct gctacaatca caccacgaca aagtccagca atgatgcgat tacgggcagg
      601 aaaatgaaat ttcagaggtt gttcgccagg tccatattca cttagaacca gatgatcatt
      661 gccgatgtag tcttgcaagc gtttattggc tttaggataa aacacatcca gtcctgttcc
      721 aatcactgca atggtttttc cgccattctg aagagctgcc atatgagctg ctgtgtcaat
      781 gcccttggcc agaccactga caataaccag ttcattttcc aagccttgaa tgactttttc
      841 aactgactta gctccctgtt tgctacaagc acgactgccc acgaccgcta ccttcgggaa
      901 tttcaggagg tcaagatttc ccttgtaaaa taaaagtaca ggcgcatcat atatttcact
      961 caaatcccaa ggataacagt catctaaaat agagaaagat ggaaattttt gaaactcttt
     1021 cgacaaatgc gcatcgtcta tctgaaaata acgttccata aaaacggctg gattacggca
     1081 acctgatata tctgcaatat cacccaacaa aagctcctga tcaacatttt caccgtattc
     1141 tagcactttc aaaatctgtt gattggtcaa acctgatttt tttaacttat agatttcata
     1201 gtttgtgatt ttcataaata actccatttc tttttctact catctatcta ttcgtaaaag
     1261 ttcgtaaaaa atcaaaaaac atccgactat tttgtcagat gttggaatct ttaattttcg
     1321 tttttaagaa tttcttctaa tttacgatag tcttggacac tatcgatctc ataaatgcta
     1381 ttgccttcta attcttcaac atagacatct agctctttga tattatcctt aaccatattg
     1441 tcccaataga gatcaacaaa ttcaccactt gcataagcct tgtcgataaa gctgacaatc
     1501 ttttctgcag ttggagcatc ccagaaggat acaccactaa ggatgcgacc tgccttgcta
     1561 tcaacaataa tgtcttgaac cttgtagtca tctccataga ccaagaacca ttcgttggta
     1621 caatcttcac gataaacact aaaataagtc gaacgtgtca aatcattgcg gaacatattt
     1681 ttaaagagat aattgtcagc atcaataaca tagctgttgg ccaattcttc ttttacaaga
     1741 tagagagagt aaaagttatt gtagtcagcg tatttatcat tgaaaacgag gcgaacaccg
     1801 tatttttctt ttaagtaatc gaattgttct ttaagataac caacaatgat gatgatgtca
     1861 ttgattcctt tttctttgag aaactcaatt tggtattcaa tcaaaggttt ttgattaacc
     1921 tgaaccaagg ctttaggggt attttcagtc ataggacgca agcgagttcc caatcccgct
     1981 gctaagataa tggctttcac acgaatctcc tttattcttt aataataata taaactccag
     2041 caatgacgac aagtgacgtc ataatggtca gcatatctag cggtgcaccc aaaaaaacaa
     2101 ctgcaaacaa gaccgtccat actacatagc tcacgttcaa gcctgtagcc ttggctggtt
     2161 gcaagcgatt gatagcgata taataagcca agtaggaaat catatcaaag gctgcaaaaa
     2221 caatcatgag acctagcaat tgtccattgg ctactgcagt aaatgactga tgagagaaga
     2281 gcacaatcac aagataggac aagaacgaag tcacttgacg gattaagagg gcttcgattt
     2341 cactcagttc actttccata gcaaaggagc taagaacact ctcacttccc catgcaatag
     2401 cacaaaccaa agcacagaga atcccaatgt agaaggaatt aacctgttca accttatagg
     2461 tctgggcaat aatccctcca ataatcaaga caatcccaaa tacagtattt ttcgaaatct
     2521 tgtgcttcaa aaagaaaaaa gccaatagaa ctgaaatcgc agggtaaata gccgatacag
     2581 atgaagctaa agaacttccg atatacttaa ctgcataaag attggcctgc ataccgatag
     2641 ggcctgctag caaggctccg atgataacac tgacattgcg aatatttaag aaaattgaga
     2701 ggcgaacttt cccttctttt accaagagaa aagctagtaa gataaagatg ctcaaaaaat
     2761 catgagctgc agccaccaca aagggtgaca aatctgtaaa aatcgaaaag atataagcac
     2821 taaccgttag acccaagccc cagaaaatac ctgagagaag gccaaaagga actccgtttt
     2881 tacttttcat ctgaacctcc ataagaagcc aaacctttaa tagctctttg gtaacgattc
     2941 acaccatagt caccaaaatc ttcaccttgc tcttccttat agacagtcca tagactccaa
     3001 atagtatctt gtaaaatttt ataaatagca atcttttcat gagaaaccgg tgtttggtca
     3061 ctctcatagt gagataagaa agtttcttcc tcttgggaag tgaattcaga ctctaaaaag
     3121 agggcagcca aatcccacat tggatcattc attgatgaat attcccagtc aatcaaataa
     3181 agtcgtcctt gaggtgattc gataaagttt tcaggcacca aatcgatatg acaagatttt
     3241 ctgtcaacac ctaagtcagc cagtcttttc tctaaggaga agactgcatt tctaacagat
     3301 tcatagttgg cataaggaat ttgttcttca atcaaggatt cgtatttttt gatttcttca
     3361 aaaggagcaa attctcctct taattcctta gcagacgtat gaatagtttg taatattgga
     3421 gtaattttgt cgaacttggt cttgattgac gttgaatcaa gcgtaatcgc agattcgata
     3481 tactcattta ctttgatacc agcttcaata tcaaaaagat aattttttac atctaagcct
     3541 aaatccttta gtagttcaag attgtacttt tcatcttgtc gattgataag cttttctgtc
     3601 cctttaccaa agaatttaac aatgtattgc ttatttgttg ttttggccaa atagttttga
     3661 ttggtcattc cacccagttg ttcaacactg aggacttcct cttcttgact aagtaaggaa
     3721 gaaatttttt ctttaatgat tttctccaca attaacctcc agtacttata cttcccactt
     3781 aaacactgtt ttaaaggctg tgtttaaatc ggttgcaaag acacgatgaa tatctttaat
     3841 ttctcttaca ggttcttcta gataaaggat atttttaaga cgattagcaa atttcttgac
     3901 ttccatcatt tggatagcat tttcaaaatc aatgcgacca gaacgagatg acccaaccaa
     3961 aatcaagccc ttttctaagg catcgcgagt attgagattg actttatatt cgctaactcc
     4021 catcatgaga atcgttccct gaggacgaat gtagcgaatc aagtcattaa tagctggtcc
     4081 agtaccatca ccaccacaac attcaaaagc atggtcaaag gccaaatctt caggaatatt
     4141 atccgtaata tagcattctt tggcaaatga gaagagttcc aacttttccc aatgacgacc
     4201 aataaccaca atctctgctt ctggcaaagt atagttgata atattggcaa ccacaaaagc
     4261 taaacttcca tctccaataa cggcgatccg ctcccgcttg ctatgagcaa gagtcaatag
     4321 acgattcata gcgtgcatgc ccacactgac aaactctgta atggctgcaa ccgtatcttc
     4381 aatagcatca taagccacca cacgatcttt agggagagaa acaaactctc tcataaagcc
     4441 atcaaatcca ctagacaaga aatgggtccc tgtcatgtag ttttcataga attcttcatc
     4501 actctgcata ggagactgat tgggaatcat gacaactttt tgaccaacct cgtaggttcc
     4561 ggtcgggtca gaaatgacgg ttccacatga ctcgtgaatc attgccattg gaagcttttt
     4621 attcaaaatc ttgggatcac gttttccctg atagtaacgc tgatccgcat gacagacagc
     4681 catgtagttg ggacggataa ggatatgatt ctcttggtca atagcctctt cctgatattt
     4741 gacattgata aacttaggct tagttagttg ataaatttga ttaatcattt tactagtctt
     4801 tctcaatcat actttttgca atcttcaaat ctgttacggt tgtaatcttc agatttgagt
     4861 attcaccttt ggccaaagcc acatcttttc ctttgatcac aaagatttta catgcatctg
     4921 tcaagatttc cttctcttca tcagaaagag atccataaag gtccatgaag tccttgcaac
     4981 ggaatgtttg aggtgtttgt ccttgataaa ggtgagcacg atttggaata tctgtaataa
     5041 attgaccatt ggtactttca acgatagtat caaccgcttc taccactgtg tccactgcgt
     5101 catgattttg ggcaagttgg atattgtcct gaatcatgcg aagtgtaata aatggacgaa
     5161 cagaatcgtg ggtaacaacg atatcctctg gagtaagcgg acgataagca tcaatggctt
     5221 caatgatgtt cttaatactt gtattgcggt cagcaccacc ctttgtaatg atgatacgtt
     5281 ccttataaag aggaagatat ttatctacaa gatcttctgc atgagaaacc cagtctccat
     5341 gaacaccaac tacaattttt tcaatacttg gctccaagac aaatttttca attgtatgaa
     5401 tcaaaatagg tcgatcacct agctctaaaa attgttttgg caagttactg atccccatgc
     5461 gtgtgccagt tccaccggca agaattcctg cataaatcat ctattttctc ctttgtctta
     5521 ctaaaaaaac ttattctctc tattatacca caatctagtc ctatcataaa agaaatactg
     5581 gtcctcccct tctagaatag gagaattatg cagttctatt attcaaagaa actagtccat
     5641 ttctagtaat gactaggaat tctgtagcaa ttactaataa atggctgcga aattttagag
     5701 tccttcagaa taatatactt tccttaaaga aaacttaaaa cttaagattt gaaacttcaa
     5761 agtgattact tgacttactg cccttcattt cttattagct aactttatga tataatgaaa
     5821 aacgaggtaa attgaatgaa aagtataaaa ttaaatgctc tatcttacat gggaattcgt
     5881 gtcttgaata ttatttttcc catcctaact ggaacctatg tcgcgcgtgt cttggaccga
     5941 actgactatg gttacttcaa ctcagtcgac actattttgt catttttctt gccctttgca
     6001 acttatggtg tctataacta cggtttaagg gctatcagta atgtcaagga taacaaaaaa
     6061 gatcttaaca gaaccttttc tagtcttttt tatttgtgca tcgcttgtac gattttgacc
     6121 actgctgtct atatcctagc ctatcctctc ttctttactg ataatccaat catcaaaaag
     6181 gtctaccttg ttatggggat tcaactcatt gcccagattt tttcaatcga atgggtcaat
     6241 gaagctctgg aaaattacag ttttctcttt tacaaaactg ccttcatccg tatcctgatg
     6301 ctggtctcta ttttcttatt tgttaaaaat gaacacgata ttgttgtcta tacacttgtg
     6361 atgagtttat cgacgctgat taactacctg attagttatt tttggattaa aagagacatc
     6421 aaacttgtta agattcacct aagtgatttt aaaccactct ttctccctct gacagccatg
     6481 ttagtctttg ccaatgccaa tatgctcttc acttttttag atcgcctctt cctcgttaaa
     6541 acagggattg atgtcaacgt tagttactat accatagctc agcgaattgt gaccgttata
     6601 gctggggttg taacaggtgc aattggagtg agtgtgcctc gtctcagtta ctatctgggg
     6661 aaaggagaca aagaagccta tgtttctctg gttaatagag gtagtcgaat ctttaacttc
     6721 tttatcattc cactgagttt tggactcatg gttttaggac caaatgccat cctactttac
     6781 ggtagtgaaa aatatatcgg aggcggcatc ttgacctctc tcttcgcttt tcgtacgatt
     6841 atcctggcct tagataccat tcttggttcc caaattctct ttaccaatgg ctatgaaaaa
     6901 cgtatcacag tttatacagt ctttgctggg ctactcaatt tgggcttgaa tagtctcctc
     6961 tttttcaacc gtatcgtggc tcccgaatac tacttactga caactatgtt atcagaggct
     7021 tctctacttg ttttctatat cattttcatc catagaaaac aactcatcca cttgggacat
     7081 atctttagct atactgttcg atactctctc ttttcacttt cctttgtagc aatttatttc
     7141 ctgattaatt tcgtgtatcc tgtggatatg gtcattaatt tgccattttt gattaatact
     7201 ggtttgatta tcttgctatc agctatctct tatattagtc tacttgtctt cacaaaagat
     7261 agcattttct atgaattttt aaaccatgtt ctagccttaa aaaataaatt taaaaaatca
     7321 taggagttta aaatgaaaca actaaccgtt gaagatgcca aacaaattga attagaaatt
     7381 ttggattata ttgatactct ctgtaaaaag cacaatatca actatattat taactacggt
     7441 actctgattg gggcggttcg acatgagggc tttatccctt gggacgacga tattgattta
     7501 tccatgccta gagaagacta ccaacgattt attaacattt ttcaaaagga aaaaagcaag
     7561 tatgaactcc tatccttaga aactgataag aactacttta acaactttat caagataacc
     7621 gacagtacga ctaaaattat tgatactcga aatacaaaaa cctatgagtc tggtatcttt
     7681 atcgatattt tccctatgga tcgctttgat gatcctaagg tcattgatac ttgttataaa
     7741 ctggaaagct tcaaactgct gtctttcagt aaacataaaa atattgtcta taaggatagc
     7801 cttttaaaag attggatacg aacagccttt tggttgctcc ttcgaccagt ttctcctcgt
     7861 tattttgcaa ataaaatcga gaaagaaatt caaaaatata gtcgtgaaaa tgggcagtat
     7921 atggctttta tcccttcaaa atttaaggaa aaggaagtct tcccaagtgg tacctttgat
     7981 aaaataatcg atttaccctt tgagaattta agccttcctg cacctgaaaa atttgatact
     8041 attttgacac aattttatgg agattatatg accctaccac cagaagaaaa acgcttctac
     8101 agtcatgaat ttcacgctta taaattggag gattaggatg caatatttag aaaaaaaaga
     8161 aattaaagaa attcaactag ccctgctgga ctatattgat gagacttgta agaaacatga
     8221 tattccttat tttctcagtt atggaaccat gcttggagcc atccgccaca aaggtatgat
     8281 tccttgggat gatgatattg atatttccct ttatcgtgag gattatgagc gtttactgaa
     8341 gattattgaa gaagaaaatc accctcgcta caaggttctt tcctacgata catcttcttg
     8401 gtacttccat aatttcgcat cgattttgga cacttctact gttatagaag accatgttaa
     8461 gtacaagcgt catgatacca gccttttcat cgatgtcttc ccaattgatc gatttacaga
     8521 cttgagcatt gtcgacaaga gctataagta tgtggctctt cgtcaactag cttatatcaa
     8581 aaaatcacga gcagttcacg gtgatagcaa actaaaagat tttcttagat tatgtagctg
     8641 gtacgctctc cgatttgtca atcctcgcta cttttacaag aaaattgatc aactagtcaa
     8701 aaatgctgta accaacattc ctcaatatga aggaggagtt gggatcggta aggaagggat
     8761 gaaagaaatc ttcccagttg atacctttaa agaactgatt ttaactgagt ttgagggccg
     8821 tatgttgcct gttcccaaaa aatatgacca atttttaacc cagatgtatg gcgattatat
     8881 gacaccacca tcaaaagaaa tgcaagagtg gtatagtcat agcattaaag cttatcgcaa
     8941 aaactgattg agggggatta tacaaactac taagatagag gttattcaaa aacataattt
     9001 tagtagaaaa tgaaatacat attcccacaa taaaacgcat catatcaagg tttttgaaaa
     9061 acctt
//
LOCUS       DAVMEI010000076         8992 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVMEI010000076 DAVMEI010000000
VERSION     DAVMEI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.076"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(32..1366)
                     /locus_tag="VYQ24_001312"
     CDS             complement(32..1366)
                     /locus_tag="VYQ24_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEV1968447.1"
                     /translation="MTYLFLLCLTLFLLTFFYFFAFNQDLIAPPVVMSVMFLISSVFA
                     LINVQNWNIEYSGLAYLLIISGIIVFSMPLLALNSPILNTKIKVTDRLIDIQFWKIAL
                     TIIVDLIILYLYRREIHNLALSHGYTGSNFQWFFRNATSYEGELTVRTSIRVLIRIID
                     VSAYIFGYTFINNFFIYSHKRSKDLLLLVPFLIFISKTLLSGGRLDIIKILIAYVVMA
                     YIQQKRKVGWDKVISHKYMRLGFVGLIAGIPTFYYSLFLSGRSTTRTVFESISTYLGG
                     SIQHFNQYIQNPIGVAEVFGDESFVAIMNILGNLGFVNYNSTVHLEFRQLGITMGNVY
                     TFFRRPWHDFGLVGMYIFSFVVGVFFAIFYLKLRKSRAGFKLDIHTIIYSYFFYWIFL
                     SSIEQYSFTTISLFTLVFIVLVYLMAFFYWNLDFHRGKLVIKLSDTSIKSEN"
     gene            complement(1367..2245)
                     /locus_tag="VYQ24_001313"
     CDS             complement(1367..2245)
                     /locus_tag="VYQ24_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1968448.1"
                     /translation="MFCYIILHYKVLEETISCVKSIKEGNSNTKQIVIIDNFSNNGTG
                     EKLQELYESDSEIDVLINHENAGFARGNNVAYQFAKEKYKPDFMVIMNNDIEIETEEF
                     EKIVTDIYRKEKFHLLGPDIFSTTYQLHQNPKRLTHYTYEEVKALNEKFKKGSQVSLA
                     LKIKCWLKSSKVLRTAIYQNRRKKKSVDYRKQVENPILHGSFIVYSRDFIEKEEYAFN
                     PNTFFYYETEILDYEAELKGYKRIYTPKIKVLHHQNVATNQVYTNLVEKTLFSNKCNF
                     ESTSYFLKLMKENEGV"
     gene            complement(2247..3071)
                     /locus_tag="VYQ24_001314"
     CDS             complement(2247..3071)
                     /locus_tag="VYQ24_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV1968449.1"
                     /translation="MNQTEIVKLIQKVELDAIKEFQKICKENNIDFFLRGGSVLGAVK
                     YDGFIPWDDDMDIAVPREGYDKLPGIFKDRIIAGKYQVLAYQYCDTLHCYFPRLFLLE
                     DERKRLGLPRNTNLGLHLIDIIPLDGAPNHSFLRKLYFGKVYWYRFLASLGTTYVGDH
                     VDMHSAKQKLIIGFFKKLGFAKLFPQNYVYRRLDNLYKKYDWKKQKYAGTINASLFAK
                     EVMPVEIWGEGVEKPFEDAFFKVPTEYDRYLKRLYGENYLHEEPSDDEKKSHLGGQ"
     gene            complement(3068..3811)
                     /locus_tag="VYQ24_001315"
     CDS             complement(3068..3811)
                     /locus_tag="VYQ24_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV1968450.1"
                     /translation="MNERIQILGITIDPLTMKETVDAVEQYVLKKHPLHLMGVNADKI
                     NQCYEDEKIKKIVNESGIINADGASVVLASKFLGTPVPERVAGIDLMKHLLELSNEKG
                     YSVYFFGAKEDVLQDMLKIFEERYPALNVVGYRNGYFSPEDEKHIQEDIKEKKPDFVF
                     VGITSPKKEYIIQSFMDNGINAVFMGVGGSFDVLSGHIKRAPLWMQNAHLEWLFRVAN
                     EPKRLFKRYFVGNVSFIRKVLRAKRGYKL"
     gene            complement(3817..5178)
                     /locus_tag="VYQ24_001316"
     CDS             complement(3817..5178)
                     /locus_tag="VYQ24_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1968451.1"
                     /translation="MSEKLAKPSVAIVQSFLVILLTYLLSAVRETEIVSTTAIVLYIL
                     HYFVFYISDYGRNFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LLLHVFLVYVLNPFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESDDVVGEL
                     VAVSVLDKPDFQHDDLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDWARNKQICEMAGLNVVTFSTTFYKTSHVIAKRVIDIVGSLVGLILCG
                     LVSIVLVPLIRKDGGSAFFAQTRIGKNGRHFTFYKFRSMCVDAEEKKRELMEQNTMQG
                     GMFKVDDDPRITKIGHFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPEQK
                     RRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVLMKD
                     GAK"
     gene            complement(5193..5882)
                     /locus_tag="VYQ24_001317"
     CDS             complement(5193..5882)
                     /locus_tag="VYQ24_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEV1968452.1"
                     /translation="MPTLEISQAKLELVKKAEEYYNALCTNLQLSGDDLKVFSITSVK
                     AGEGKTTTSTNIAWAFARAGYKTLLIDADMRNSVMSGVFKSRERITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIVVDTAPVGVVI
                     DAAIIMQKCDASILVTKAGETKRRDLQKAKEQLEQTGKSCLGVVLNKLDTSVEKYGSY
                     GSYGNYRKQKK"
     gene            complement(5892..6584)
                     /gene="cpsC"
                     /locus_tag="VYQ24_001318"
     CDS             complement(5892..6584)
                     /gene="cpsC"
                     /locus_tag="VYQ24_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1968453.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKHKLIILLVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGTYLVKDYREIILSQDVLEKVATNLKLD
                     IPAKTLTSKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPAMTPSSPNVRRNTLVGFLGGAAVTVITVLLIELLDTRVKRPEDVEDVLQMP
                     LLGVVPDFDKMK"
     gene            complement(6593..7324)
                     /gene="cps4B"
                     /locus_tag="VYQ24_001319"
     CDS             complement(6593..7324)
                     /gene="cps4B"
                     /locus_tag="VYQ24_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEV1968454.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTSDVLDKLEKNRIPTLNNS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELINMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLERDLVHIIASDMHNVDSRPPHMAEAYDLVS
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            complement(7326..8771)
                     /locus_tag="VYQ24_001320"
     CDS             complement(7326..8771)
                     /locus_tag="VYQ24_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1968455.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VDQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVYNDQ
                     DFTSLHGKFHFPVGNVHLDSEQALGFVRERYSLADGDHDRGRNQQKVIAAILQKLTSS
                     EALKNYSRIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGMGRTDLPSY
                     AMPDSNLYMMEINDSSLASVKTAIQDVLEGR"
BASE COUNT         2849 a         1892 c         1439 g         2812 t
ORIGIN      
        1 tcatcaacta tcctctctat ttttattgtt ttcagttttc cgatttaata cttgtgtcag
       61 agagtttaat tactagtttt cctctgtgga aatccaaatt ccaatagaag aaagccatta
      121 ggtaaaccaa gactataaat acaagtgtaa acaaactgat tgttgtaaac gagtattgct
      181 caatggacga taaaaaaatc caataaaaga aataagagta aataattgta tgaatatcca
      241 atttaaaacc agcacggctc tttcttaatt tcaaataaaa aatagcaaaa aacacaccga
      301 caacaaagga aaagatatac ataccgacta acccaaagtc atgccagggt cttctaaaga
      361 aagtataaac gttacccata gtaatcccta actgccgaaa ttctaagtga acggtactat
      421 tgtaattgac aaagccaaga ttacccaaaa tattcataat agctacaaat gactcatcgc
      481 caaaaacctc agctactcca ataggatttt gaatatactg attaaaatgc tgaatcgaac
      541 ctcctaaata ggttgaaata ctttcaaata cagttctagt cgtagaacgt ccagataaga
      601 ataaagaata gtaaaaggta ggaatcccag ctatcaagcc tacaaaacca agtctcatat
      661 atttatggga gatgacctta tcccagccaa cttttcgttt ttgctgaata taggccatta
      721 caacatacgc aattaaaatt tttataatat ccaatctacc cccagataat aaggttttag
      781 aaataaaaat caagaatgga actaagagca gtaaatcttt agagcgttta tgactataaa
      841 tgaagaaatt attaataaaa gtatatccaa aaatataagc agatacgtca atgatacgaa
      901 tgaggacccg aatcgaagtt cgcactgtta gctcaccttc ataactggta gcatttctaa
      961 agaaccactg aaaatttgaa cccgtataac catggctaag tgcaagatta tgaatctctc
     1021 tcctataaag atatagaata atgaggtcaa ctataatagt aagagctatt ttccagaatt
     1081 ggatatcaat gagccgatcc gtcactttaa tcttggtatt tagaataggc gaattaagag
     1141 ccaagagagg cattgaaaaa acaataatgc cactaataat caaaagataa gctaaaccac
     1201 tatactcaat attccagttt tgcacattaa taagggcaaa tacagaacta atgagaaaca
     1261 taacagacat gacaactggc ggagctatca aatcttgatt gaaagcaaaa aaatagaaga
     1321 aagttaataa gaataaggtc aggcagagta aaaataaata agtcatttac acaccctcgt
     1381 tttctttcat caacttcaaa aaataactag tggattcaaa gttgcatttg tttgaaaaca
     1441 aggttttttc tactaagttt gtatagactt gattggttgc aacattttga tggtgcaaga
     1501 ccttaatctt cggtgtataa attctcttat atccttttaa ctcagcttca taatctaata
     1561 tctctgtttc ataatagaag aaggtattgg gattaaaagc atactcctct ttctcaataa
     1621 aatctctaga atatacaata aacgaaccat gaagaattgg gttttctacc tgttttctat
     1681 agtctactga tttcttttta cgcctatttt gatagattgc tgtccgaaga actttactag
     1741 acttcaacca acatttaatt tttaatgcta gactaacttg gctccctttc ttaaattttt
     1801 cattgagagc cttaacctct tcataagtat aatgcgtcaa ccgttttggg ttttggtgaa
     1861 gctgatacgt cgtcgaaaag atatctggtc ctaacaaatg gaatttttcc ttccgataga
     1921 tatctgtcac gattttttca aactcttctg tttctatctc aatatcatta ttcatgataa
     1981 ccataaaatc aggtttgtac ttttccttag caaactgata tgctacatta ttacctcgag
     2041 cgaaaccagc attttcatgg ttaatcaaga catcaatctc tgaatctgac tcatacagct
     2101 cttgtagttt ttcacccgta ccgttattag aaaaattatc aataataacg atctgctttg
     2161 tattggaatt gccttctttt attgatttaa cacaagaaat agtttcctct aagactttgt
     2221 aatgcaaaat aatataacaa aacaaattat tgtcctccta aatgcgattt cttttcatca
     2281 tcactaggct cttcgtgaag atagttttct ccgtaaagtc ttttcagata gcggtcatac
     2341 tccgtgggaa ctttaaagaa ggcatcctca aaaggcttct ctactccttc tccccaaatc
     2401 tctactggca tgacttcttt ggcaaacaga gatgcattaa ttgtcccagc atacttctgc
     2461 tttttccaat catacttttt atagagatta tccaagcgtc tgtacacata attttgaggg
     2521 aatagttttg caaatcctag ctttttaaag aaaccaatga ttagtttttg cttagcggaa
     2581 tgcatatcca catggtctcc aacataggta gttcctaaac ttgctaaaaa acgataccag
     2641 tatactttac cgaagtaaag ctttcttaaa aatgaatgat ttggggcacc atccaaagga
     2701 atgatatcaa tcaaatgcaa acctaaattg gtatttcgtg gcaatcccaa acgttttctt
     2761 tcatcttcta aaaggaataa gcgaggaaag tagcaatgta aagtatcgca atattgataa
     2821 gcaagaacct gatatttgcc tgcgataatt ctatccttaa agatacctgg aagtttatcg
     2881 tagccttcac gaggcacagc aatatccatg tcatcatccc atggaataaa gccatcatat
     2941 ttgactgccc caagtacact ccccccacga aggaagaaat ctatattgtt ctctttacaa
     3001 attttttgaa actcttttat agcatccaat tcaactttct gaatcaattt aactatttct
     3061 gtttggttca aagtttatat ccccttttcg ctcttaaaac ttttcttata aatgaaacat
     3121 tccctacaaa ataacgctta aagagacgtt taggctcatt tgccacacga aacaaccact
     3181 ctaggtgagc attttgcatc cataatggag ctcgtttgat atgccctgat agtacatcaa
     3241 aacttccacc aacccccata aacacagcat taattccatt atccatgaat gattggataa
     3301 tatattcttt cttaggagaa gtaattccta caaacacaaa atctggtttt ttctctttaa
     3361 tatcttcctg aatatgtttt tcatcttcag gcgaaaaata tccatttcta tagccaacaa
     3421 catttagagc tggatatctt tcttcaaaaa tttttaacat atcttgtaaa acgtcttctt
     3481 ttgctccaaa aaagtagacc gagtatcctt tttcattaga caactctagt aaatgtttca
     3541 tcaagtctat tccagcaact cgctcaggaa ctggtgttcc taaaaactta ctcgctaaaa
     3601 ctactgaagc accgtcggca ttaattattc ctgattcatt aacgattttt ttgattttct
     3661 catcttcata gcactgatta attttatccg cgttaactcc catcaagtgc aaagggtgct
     3721 tctttaaaac atactgttca acagcatcca ctgtttcttt catcgttaat ggatctatag
     3781 taattcctaa tatttgaatt ctctcattca tatgaactat ttcgctccat ccttcatcaa
     3841 tacaacctta actgtcttca ataaaatttc aatatctttc cagattgtcc aaccatcaat
     3901 ataggccaca tctaatttga caacttcatc gaaatttttg atttcacttc gtccgctgac
     3961 ctgccataaa cctgttatgc caggtttaaa acttaggcga cgtttttgtt ctggggtata
     4021 gtgctcatac tcgtccactg ttggtggtcg tgtacctacc aaactcatat ctccctttag
     4081 aacattataa aactgtggta gctcgtccaa gctcgtcttc cgtataaaat gaccaatttt
     4141 cgtgatacga ggatcatcgt ccaccttaaa cattccaccc tgcatggtat tttgttccat
     4201 gagttctctt tttttctcct cagcatccac acacatagag cgaaacttat aaaaagtaaa
     4261 atggcggcca ttttttccta tacgcgtctg agcaaaaaaa gcagaacccc catcctttcg
     4321 aatcaaagga accagcacaa tactgactag accacataat atcaaaccta ccagggaacc
     4381 gacaatatca ataacccgct tagcaatcac atggctagtc ttataaaatg ttgtagaaaa
     4441 agtcacaaca tttagtcctg ccatctcaca aatttgcttg ttacgtgccc aatcgaaagc
     4501 atttagattg actgttacat caattcccat cgtttcaaac tgagagacaa gctctccaat
     4561 attgtatttt tcacttggaa gattgataaa gacttcatcg accacctcat gagtcgcaaa
     4621 atttactatc tccccctctg ctacaacctt taaatcatca tgctgaaaat ctggtttatc
     4681 taaaacactg acggctacca actccccaac aacatcatct gattctatta gtctatccag
     4741 taccttttca acacgagaag ttgctgtaag taggagaatc ttcttacttc ctttaaagtt
     4801 gggataagcc cgcttccaat accacttgat aaatgggttt agcacataga ctaagaaaac
     4861 atgtaataag aggaagtaaa tcatgcctct tctggaaata ctaaatcgat cttctaagaa
     4921 aaaattagaa atactaatcg ctagcgcaaa gaataggata tatttcaatg tctggacaag
     4981 ttcaatcaaa tatccccttt taaagaaatt ccgtccataa tcactgatat aaaagacaaa
     5041 atagtggagg atataaagta caatagctgt tgttgaaaca atctctgttt ctctcacagc
     5101 gctaagtaga taagtcaata aaataacaag aaaactctgg actatggcta ctgaaggctt
     5161 tgctagtttt tcactcattt ctatccctca acctattttt tctgtttcct gtaattccca
     5221 taactcccat aagaaccgta tttctctact gaagtatcga gtttattcaa cacaactcct
     5281 aaacacgact tcccagtttg ttccaactgt tctttcgctt tttgaagatc ccgtcgtttt
     5341 gtttcacctg ccttcgtcac taaaatagaa gcatcacatt tctgcataat gatagccgca
     5401 tcaatcacga caccgacagg ggcagtgtct acgacgatat agtcaaaata tttacgcaag
     5461 gtttcaagca ttgtactgaa attcttactt tgaagaaggg ctgtcggatt tggtgacaca
     5521 gagccagcct gaatcacaaa gagattctca atattggtat cgcaaagccc ctgtgacagg
     5581 tctgttgtcc ctgatagaaa ttctgttagt ccagtaatcc tttcccttga tttaaagaca
     5641 cctgacataa cggagttacg catgtctgca tcaatcagaa gcgttttgta acctgcacgc
     5701 gcaaaagccc aagcgatatt ggtggaagtc gttgtttttc cttctcctgc tttcacagaa
     5761 gtgatagaaa atactttcaa atcatctcca cttaactgta ggttcgtgca caaagcatta
     5821 taatattcct ctgccttttt cacaagttct aatttcgcct gtgagatttc taacgttggc
     5881 ataacttcct cctatttcat tttatcaaaa tctggaacga ctcctagaag tggcatttgc
     5941 agtacatctt caacatcttc aggacgtttt acacgggtat cgagcaactc aatcaaaaga
     6001 acagtaatta ctgttacggc ggctcctcca agaaaaccaa ctaaggtgtt gcgtcgaaca
     6061 tttggagaag agggagtcat agctggtcgc gcttcttcaa gcgtcgttac atcagatact
     6121 cgcgttacag cgatgatctt ttctgcagca acttctcgta gagaattagc aatgcgactg
     6181 gcttcctctg gttgtttatc cttgacagag attgagacga tacgagtgtc ggctggtaca
     6241 gtcacttgca ctttgctagt taacgttttt gctggaatat ccaatttcaa atttgtcgct
     6301 accttttcca atacatcttg cgaaaggata atttcgcggt agtcttttac cagataagtt
     6361 cctgcctgca agtcctgatt cgtcagtccc ggcttatctc cttgattacg gttgactacg
     6421 taaatacgcg tggtgcttgt atattctggc ttaacaataa aagtgctata tgcaaaagct
     6481 cccgcccctg tcacaagtgc cactaataaa attattagct tgtgtttcca caaggtttta
     6541 agtaattgaa atacatcgat ttctatcgtg ttttgttctt tcatcatttc tcctaaatta
     6601 gttgatccat tacaattttt cgaggattat ctataaaaag ttcctgagcc ttcgcttctc
     6661 cgtatttttg ggaaacaagg tcatatgctt ctgccatatg gggaggtctg ctgtccacat
     6721 tatgcatatc acttgcaatg atatgaacca agtcacgctc caaaaaatac tgagctcttt
     6781 ttttcatgaa tttataacgt tctccaaaaa gtttggattt gaggacatgt gaactattta
     6841 cttgcgtgta acagcccata ttgatcagct ctcgaacgcg tttttcatta ttttcaagtg
     6901 catcatagcg ctcaatgtgg gcaatgactg gagtaattcc caacatcaat attttactca
     6961 aggcactatg aatatcgcga taaggggtgt tcatactaaa ctctatcaag gcataacgac
     7021 tattattgag ggtcggaatc cgattttttt ccagcttatc caaaacatct gatgtgtagt
     7081 aaatttcagc cccgtaagca atgaccaagt cactcgccac ttccttagct atttcccgaa
     7141 cctgaagaaa gttttctgct atcttctctt ccggagtttc aaacatgccc ttgcgacggt
     7201 gagaggtaga gacaatggtt cgcaccccct gcctgtagga ttctgccaag agagccttgc
     7261 tttcctctct tgacttggga ccatcatcta catcaaagac gatgtgcgaa tgaatatcaa
     7321 tcatttcatc tgccctccaa cacatcctga atagcagttt tgacagatgc aaggctactg
     7381 tcgttaattt ccatcatata gaggttacta tctggcatcg cataggaagg aagatccgtc
     7441 cgtcccatac ccttcaagtc ttgcgaattc actttgtacg ttccaccact ttctaactga
     7501 gcattgacca agtttatcat ggtctcaagt ggcatgtttg tttggataga atcttgcaag
     7561 ctatcaatga tcctactata atttttcagc gcttcgctcg aagttaattt ttgaaggatg
     7621 gccgcaatca ccttttgttg attgcgcccg cggtcatgat caccatccgc tagggaatag
     7681 cgctcacgaa caaatcccag agcctgctca gaatctaagt ggacatttcc aactgggaaa
     7741 tggaacttcc cgtgtagaga tgtaaaatct tggtcattat aaacatctac cccacccaac
     7801 aaatcgatca atttcaagaa agaagtgaag ttcaatcgca catagtaatt gatatccact
     7861 ccatagagat tttctaaagt gtggatggac gaatcaactc catagatgcc cgcatgggtc
     7921 aatttatctt tttgattatt cccaccatct gcgattggta cataagcatc acgtggcgtt
     7981 gtggtcaaga ggattttctt ggtatctcga ttgacagtca tcaggatatt gacatctgat
     8041 cgcgacaccg aactaatagg accataggtg tcaattccac taacatagat attgaaagac
     8101 tgattcttag acgtcttagg agcttctact tttttagtga atcccttggt ataaatcttt
     8161 tttatcttcg atgcgtagtc tggatactct gactcgatga tattttcaaa gacactattt
     8221 aagacaatgg ccttagtctc ccctgcaatc aaactcttgt aagctgccaa gtaagacgaa
     8281 ctctggtcga ccgtcaaatc ggtattctga cttgacttaa tatcagctag tagtttttga
     8341 atattttcat tatcagtccc agtcggtgct gtcacactcg tcagttgcgt aacattttcg
     8401 atctcactat ctgctaaaac agcgacactg attgaatatt ctgagtaatt agaagtcgca
     8461 tttaaacgat tggtcagtcc aacaaactgc tgtactgcaa agagcgacac agagctgaca
     8521 aggatagaga acagcaacag aaaaatagta aacttttcag cttttttata gataatcaag
     8581 agtagcccta ccaaggcaac tagtaggact aacgcagtta ccactagatt aagatatcta
     8641 aaagcaagga tattgtactt aaagattaag aacaataaaa aacaaattaa caataaataa
     8701 atagtcagca aaacgatatt aacacttcgc ttcactttct gtgaacgtga ttttttaaaa
     8761 cgtctactca tgattaacac ctataccttg aacatcatac gattatatca cttttttacg
     8821 gtaatgtcta catctttttt actatctgca tctttaagta tcttaagtag acttcccgcg
     8881 aaacaaaaat atagtagatt gaaacaagaa taggataaat caatcaggac agttaaatcg
     8941 atttctaaca atgttttaga agcagaggtg tactactcta gtttcaatct aa
//
LOCUS       DAVMEI010000077         8922 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVMEI010000077 DAVMEI010000000
VERSION     DAVMEI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.077"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..487
                     /locus_tag="VYQ24_001321"
     CDS             <1..487
                     /locus_tag="VYQ24_001321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968456.1"
                     /translation="AMSTLILGELLSSLIISRKKQETSPLKNGYSIMLGSTLAMSFLG
                     FSTFFSNKPIAFSFILLSLFSMTFIESFFTIPLQVWYVTEIPEKIQGRIFAFLGAIIS
                     FFSPLSTLMFSGLYEIKTLPLQQINAIIIFSAVSIRLISTIYLKFIKKVNLADAHVLK
                     Y"
     gene            703..845
                     /locus_tag="VYQ24_001322"
                     /pseudo
     CDS             703..845
                     /locus_tag="VYQ24_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1044..1454
                     /locus_tag="VYQ24_001323"
     CDS             1044..1454
                     /locus_tag="VYQ24_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HEV1968457.1"
                     /translation="MEAIYQRDSDQDGLTDAQELALGTNPLSADSDGDGRSDLVEVEE
                     GTNPLEKDLQDIDQTSITEPSSVFMEMKQKISDMMESHYKEFIQALISIETGIENQQD
                     LEDLYTYYMRTDSVSLLSSDLETSPQEVEMEIEL"
     gene            1502..7726
                     /locus_tag="VYQ24_001324"
     CDS             1502..7726
                     /locus_tag="VYQ24_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1968458.1"
                     /translation="MEVMQLLAMFRGTIPKDREKMDLFLRYQAQHFDEKWQDLVENFL
                     TEEGKIEEIPHVYSFDQDIVSFLEASSENNDQDLESYTRSFGQAGLGKLSQLSNFEKN
                     LVLEVAIYNLSTRFYIQSEKEKLTPLSELVCHQNQDVNLVNVYRVANNLSDRISRDIE
                     EFLLMVDSKEVKKEVLEIHFEGKEGDVLAYLGSELMATLDSVTELVHHEENYQQLPLT
                     QKLKIITHFDDVKAKIEKSKRVAEVVLPLDNIEQVEENVEVHSLSKVDKIVEEALREY
                     PIGSQVSYRGQVFQLVSIENAQLNDLVRLELFNDSNQLFEENPILYLNSLEEIEQVLS
                     LVELEKEDSEIEIESPSESQEIDLFSYLEEENEKDKDKETKPLISGIEETEVPVQDFV
                     FPDDLEDFYPKTNREKIETNIAAIELVKRLEKEGRQANPEEQELLAKYVGWGGLANEF
                     FDELNPKYETERLTLRSLVSKSEYSTMKQSSLTAYYTDPMIIRQIWQKLLDDGFGGGR
                     ILDPSMGTGNFFAAMPRSIRDKSELYGVELDSVTGAIAKQLHPNVHIEVRGFEEVPYQ
                     NNSFDLVLTNVPFGNFRIADKNYDKPYMIHDYFVKHSLDLVRDGGQVSIISSIGTMDK
                     RTDNVLQEIKSNTHFLGGVRLPDTAFKKIAGTRVTTDLLFFQKDQAKNLNEEELVFSG
                     SIPFEEDKRVWINPYFDGKYNTQVLGKYEVRNFNGGTLNVKGGSETLATDIMKALENV
                     EEPKPIDNSLKAPVFIQEEVDNSIPSRIREDLALYSFGYEGNQIYYRDTHGIRKSSKV
                     DEISYYVDEKGDFKAWDSSLSEHKIDRFVKLHLTDEEALDVYKSEEASKRGKYKGLFK
                     KTVFYENPLSDKDISRIKGMVDLRETYQSLIEIQRHQDYSRTDFQVLLSKLNHDYDRF
                     VSQFGYLNASVNRNLFDSDDKYSLLASLEDEYIDSKDQKVKYKKSLAFEKALVRPERV
                     ITRVSTALDALNSSLSDGRGVDLDYMVSIYPEHSQAAILDELGDQVLMDPESYLRGER
                     KYLSKNQFLSGDILNKIEVVQLLVEENNQEYDWNHALDLLESVRPPRIHLADIEFKIG
                     SRWIPQSVYGKFAFECFTNREFELSSPDVEQVIEVNPVDGQVHLRTSFAYRYPSAKDS
                     SLGVSGSRYDTGRKIFENLLNSNQPTITMTFTEGEKKKTITDLEKTSVLRAKEQHLQE
                     LFQEFVSRYPEVQQVIEESYNRLYNRTVSREYDGSHLVIDGLAQNISLRPHQENAIQR
                     IVEEKRALLAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLSAQFGQEIMKFFP
                     TKKVFVTTKKDFVKARRKQFVSRIITGDYDAIVIGDSQFEKIPVSKERQMNYIEDKLN
                     ELREIKTHSENKYTVKEAEQSISGLEKQLEELQRFNRDSFIDFENLGIDFLFVDEAHH
                     FKNIRPITGLGNVAGITNTTSKKNVDMEMKVRQIQEEHDFKNIVFATGTPVSNSISEL
                     YTMMNYIQPDILKRYQVDYFDSWVGAFGEIQNSMELAPTGDKYQPKKRFKKFVNLPEL
                     MKIYKETADIQTQDMLDLPVPEAHIIPIESELTENQKLYLEELVMRSDMVKCGTVDPS
                     QDNMLKITGEARKLAIDMRLLDSSYSLADNHKLLQVVDNVERIYREGMENKATQMIFS
                     DIGTPKKKDNGFDVYSEIKALLVDRGIPSKEIAFVHDANSDEKKNSLSRKVNAGEVRI
                     LLASTEKGGTGLNVQSKMKAVHHLDVPWRPSDIQQRNGRIIRQGNENKEVDIYHYITK
                     GSFDNYLWATQENKLRYIKQIMTSKEPIRAAEDIDEQTMTASDFKALATGNPYLKYKM
                     ELENDLTLLENQRRAFQRSKDHYRHTISYCEENMPILEKRLSKYEGDIQQSEMSKDQA
                     FSMTVGKQAFEQRAEAGESLHRLIRHNQSDSKEFRTLASYRGFDIKMLSLPTNQPLPE
                     TFSVKIVGENQYSVSLDLYSPLGTIQRLQHTIDHIKEDQVKTQNLLDELQDKLTTAKV
                     EIEKNFPKEEDYQTKKAEYDVLAPLIETETDLDIIDQALRQFHEKGKEKQEQLSFELD
                     "
     gene            7801..8085
                     /locus_tag="VYQ24_001325"
     CDS             7801..8085
                     /locus_tag="VYQ24_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968459.1"
                     /translation="MMEDTYYQLEEALVQGFQTPEEYQAYKELKEHYEEVTGDYSFSK
                     RELTSQLEIALQNHRGVDFEDYEKEDYLELVQKLEEFDSSLATHYRQLID"
     gene            8099..8419
                     /locus_tag="VYQ24_001326"
     CDS             8099..8419
                     /locus_tag="VYQ24_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968460.1"
                     /translation="MNDLLLIPVIFLAVGGILILLWRIFLIASGLFLIGFVSFLIFVE
                     VYGIYLFFTETELYTEDLAQNGLFGFTTFFIIFNLTLLVLAGWAGYKRKRHNNVLANQ
                     RDFW"
BASE COUNT         3037 a         1414 c         1808 g         2663 t
ORIGIN      
        1 tgcaatgtca acactgattc ttggtgaatt actatccagc ttaattattt cacgaaaaaa
       61 acaagaaact tcaccattaa aaaatggtta ttccattatg ctaggctcaa cgctggcaat
      121 gtcattttta ggattcagca catttttttc taataaacct atagcattta gttttattct
      181 gctctctctt ttttctatga catttattga atcttttttt acaatacctc ttcaagtttg
      241 gtatgtgact gaaattccag aaaagattca aggaagaata tttgcctttt taggagctat
      301 aatttcgttc ttttcacccc tctctacatt aatgtttagt ggattatatg aaataaagac
      361 attacctctg caacagatca atgctataat tattttttca gcggtctcta tacgactgat
      421 atcaacaatc tatttaaagt ttattaaaaa ggtaaatttg gcggacgccc atgttctaaa
      481 atattagtca gtgatatgtt taaaaaataa ccaatagaag cgtataaaag tttaaattca
      541 aaattttact ggcctctctt tttcatgaac acaaatctcc tctaatcgtc tctctgactt
      601 tttaggactg aatcaccttc aaagaccacg aagacgactt ttttgaatgt ccatctggtt
      661 catcatctta ggaggtttat ccactatgct tagacgtttt gtaacccaaa agcatctcac
      721 gctctacttt ctagcctaga atctttaaaa atccgaatcg aactattatt attgcgaccc
      781 acaatcctct tatttgggaa caagtggacc aagttattcg agttaccgat ttatcacata
      841 gatgatatgg caagattcag ttagaagaaa gtttttgatt atatacagca taaatttcta
      901 aaatctgtag tacttgttta taatcacaat attatttgag aacgcagttt tggctcgttc
      961 tctttttttg ttgtgatact tctttcaagg aatatttggt aggattggag tgaaaaaaga
     1021 ttaactagga aagaaggaag tatatggaag ccatttatca acgtgattcg gatcaagatg
     1081 gactgactga tgctcaagaa ttggcgctag gaaccaatcc tcttagcgca gattctgatg
     1141 gtgatggtcg ttcagattta gtggaagtag aagaaggaac caatccctta gaaaaggatt
     1201 tacaagacat agaccaaaca agtataactg aaccgtcttc agtatttatg gaaatgaaac
     1261 aaaagatttc agatatgatg gagagtcact acaaggaatt tatacaggct ctgattagta
     1321 ttgaaacagg gattgaaaac caacaagacc tagaagactt atatacttac tacatgagaa
     1381 cagattctgt ttctcttttg tctagtgatt tagaaaccag tcctcaagag gttgaaatgg
     1441 agatagagtt gtaggggaat catgtgattc tcctattttc gtatagatga aaggagcgag
     1501 tatggaagta atgcaattat tggctatgtt tcgtggaaca attccaaaag atagggagaa
     1561 aatggatcta tttcttcgtt atcaagcgca acattttgat gagaaatggc aggatttggt
     1621 agagaatttt ttgactgaag agggcaagat agaagagatt cctcacgtct attcttttga
     1681 tcaagatatt gtttctttcc tagaggctag ttctgaaaat aatgaccaag atttagaaag
     1741 ttacacaaga agttttgggc aagcgggtct aggtaaatta tctcaattaa gtaattttga
     1801 gaaaaacttg gtgctagaag tcgcaatcta taacctttcc actcgatttt acatacaatc
     1861 tgaaaaagaa aagctaacac cattaagtga gcttgtatgt catcagaatc aggatgtcaa
     1921 tttagtcaat gtctatcggg ttgcgaataa tctatctgac cgcattagta gagatataga
     1981 ggagtttctt ctaatggttg attccaaaga ggttaaaaaa gaagttcttg agattcattt
     2041 tgaaggaaaa gagggagatg ttctagccta tttgggttct gaattaatgg ctactttaga
     2101 tagtgttacg gaacttgtcc atcatgaaga aaactaccaa caactcccac tgacacaaaa
     2161 gctgaagatt attactcact ttgatgatgt aaaagctaaa atcgaaaagt ctaagcgagt
     2221 agcggaagtg gtcttacctt tagataatat tgagcaggta gaagaaaatg tggaagttca
     2281 ttccctatct aaagtagata aaattgtaga agaagctttg agggaatatc caatcggttc
     2341 acaagtaagt tatagaggac aagtatttca gttggtttcg attgaaaatg cacagttaaa
     2401 tgacttagtt cgcctagagc tattcaatga ttccaaccag ttatttgaag agaatcctat
     2461 tttatattta aacagtttgg aagagattga acaagtattg tctctggtag aacttgaaaa
     2521 agaagattca gagattgaga ttgaatcacc aagtgaaagc caggaaatag atttgttttc
     2581 ctatctggaa gaagaaaatg aaaaagataa ggataaggaa acaaaacctt taatttcagg
     2641 tatagaagag acggaagtcc ccgttcaaga ttttgttttt ccagatgatt tagaggactt
     2701 ttatcctaag acaaatcgtg aaaaaattga aacgaatatc gccgcaattg aacttgttaa
     2761 aagattagaa aaagagggac gacaagcgaa tccagaagaa caagagctac tagccaagta
     2821 tgtcggctgg ggtggtcttg ccaatgaatt tttcgatgaa ctcaatccaa agtatgaaac
     2881 agaacgttta actcttagga gtttagtaag taaatcagaa tactccacca tgaaacaaag
     2941 ttctctcacg gcctattata cagacccaat gattattcgc cagatttggc aaaaattact
     3001 ggatgatggt tttgggggag gaaggatatt agatccttct atggggactg ggaacttctt
     3061 tgcggctatg cctagaagta tacgagataa atcagaactc tatggggttg aattagacag
     3121 tgtgacaggc gcaatcgcaa aacaactcca tccaaatgtc catattgaag tgcgaggatt
     3181 tgaagaagtt ccctatcaaa ataatagttt tgatttagtc ttaacgaatg taccatttgg
     3241 aaattttcgc attgccgata aaaactatga taaaccttat atgattcatg attactttgt
     3301 caaacactca cttgatttag taagagacgg aggacaagtg tcgattatct catctatcgg
     3361 gacaatggat aaacggacag ataatgtctt acaagagatt aaatccaaca ctcatttttt
     3421 agggggagtt cggttgccag atacggcttt taaaaagatt gcaggtaccc gagtgactac
     3481 agatctcctc ttctttcaaa aggaccaagc aaagaatctt aatgaggagg aacttgtctt
     3541 tagtggctct attccctttg aggaggataa gcgtgtctgg atcaatcctt attttgatgg
     3601 gaaatacaat acacaagttt tgggtaaata tgaagtacgt aattttaatg gaggaaccct
     3661 caatgttaag gggggatcag aaacattagc tactgacata atgaaagcat tagagaatgt
     3721 ggaagaacct aaaccaattg ataattcttt gaaagcacct gtttttatcc aagaagaagt
     3781 ggataattca atcccaagtc gtatacgtga ggacttagcg ctctattctt ttggatatga
     3841 gggaaatcaa atttattacc gagatacgca tggcattcgg aaaagttcaa aagtagacga
     3901 aattagttat tatgtagatg agaagggaga ttttaaagct tgggacagtt ctttgtctga
     3961 acataaaata gatcgattcg tgaaacttca tttgacagat gaggaagcac tagatgttta
     4021 caagtcagaa gaagcgagta aaagagggaa atataagggc ttgttcaaaa aaactgtctt
     4081 ttatgaaaat cccttatcgg ataaggatat tagtcgtatt aagggcatgg ttgatttgag
     4141 agagacctat caatccttaa ttgaaattca acgtcatcaa gattatagtc ggacagattt
     4201 tcaggtatta cttagtaaac tcaatcatga ctatgaccgc tttgtaagtc aatttggata
     4261 cttgaatgcc tcagtcaatc ggaacttatt tgatagtgac gataagtatt ctttactagc
     4321 aagtttagaa gatgaataca ttgattctaa agatcagaaa gtaaaatata aaaaatcttt
     4381 agcctttgag aaagcattgg ttaggccaga gagagtgatt acaagagtat caacggcttt
     4441 agatgcctta aactctagtt tatcggatgg tagaggggtt gatttagact atatggtatc
     4501 aatttatcct gaacatagcc aagctgctat tttggatgag ttaggagacc aggttttaat
     4561 ggatccagaa agctatttaa gaggagaaag aaaatatctt tctaagaacc agtttttatc
     4621 aggagacatt ctcaacaaga tagaagtagt tcaactatta gtagaggaaa acaaccaaga
     4681 atatgattgg aaccatgctt tagatttgtt agaatctgtt cgccctccac ggattcatct
     4741 ggcagatatt gagtttaaaa tagggtcacg ttggattcct caatctgttt atggtaaatt
     4801 tgcttttgaa tgttttacca atcgtgaatt tgaattgtct tcgccagatg ttgaacaagt
     4861 cattgaagtg aatcctgtcg atggccaggt tcatttaagg acatcatttg cttatcgcta
     4921 tccaagtgca aaagatagta gtcttggagt cagtgggtca cgttatgata caggaagaaa
     4981 gatttttgag aatttactta attcgaacca accgacgatt accatgactt ttacggaagg
     5041 agaaaagaaa aagaccatca cagatttgga aaaaacctct gttctaagag cgaaagagca
     5101 gcatttacaa gaactctttc aagaatttgt ctcacggtat cccgaagtcc aacaagtcat
     5161 tgaggaaagc tataatcgtc tttataatcg aacggttagt cgagagtatg atggtagcca
     5221 tctagtcatt gatggcttgg cacaaaacat cagtcttcgt cctcaccaag agaatgccat
     5281 tcaaagaatc gtagaagaaa aaagagcctt gttagctcat gaggtaggtt caggaaagac
     5341 cttgaccatg cttggtgctg ggtttaaatt aaaggagttg gggatggttc ataagccctt
     5401 gtatgtggtg ccttctagtt tgtctgctca atttggtcaa gaaatcatga aatttttccc
     5461 tactaaaaag gtctttgtga ccactaagaa agattttgtg aaggcgagaa gaaaacaatt
     5521 tgtatcacgt attattacag gagattacga tgccattgtc attggggatt ctcaatttga
     5581 aaaaatccct gtcagtaaag aaagacagat gaattatatt gaggataagc tcaatgaact
     5641 acgagagatt aaaacacatt ctgaaaataa gtatactgtc aaagaagcag aacaatcaat
     5701 aagtggtcta gagaaacaat tggaagaact gcaacgcttt aatcgtgata gttttattga
     5761 ttttgagaac ttaggaattg attttctctt tgtggatgaa gcacatcact ttaaaaatat
     5821 tcgtccgatt acgggacttg gaaatgtagc agggattacc aatacaacct ctaagaagaa
     5881 cgtggatatg gaaatgaagg ttcgacagat tcaggaagaa catgatttta agaatattgt
     5941 ttttgcgaca ggaacacctg tttccaattc tattagtgag ttgtatacta tgatgaacta
     6001 cattcaaccg gatatcttaa aacgctatca agttgattat tttgactctt gggtaggtgc
     6061 ttttggagaa attcaaaact ctatggaatt agctcctaca ggggataagt accaacctaa
     6121 gaaacgattt aaaaagtttg tcaatctacc tgagttgatg aaaatctata aagaaacagc
     6181 cgacattcaa acacaagata tgttggattt acctgttcca gaagctcata ttatccctat
     6241 tgagagtgag ttaactgaaa accagaaact ctatttagaa gaattagtta tgaggtcaga
     6301 tatggtcaaa tgtggaacag ttgatccgag ccaggataac atgttaaaga taacaggtga
     6361 ggcacggaaa ctagctattg atatgcgttt attggactct agctatagtc tagcagacaa
     6421 tcataaactg cttcaggtag ttgataatgt tgaaagaatt tatcgtgagg gaatggaaaa
     6481 taaggctact cagatgattt tttcagatat tggcacccct aagaagaagg ataatggctt
     6541 tgatgtctat tctgaaataa aggctttatt agtggataga ggaatcccta gtaaggaaat
     6601 tgcctttgta catgatgcca atagtgatga aaagaagaat agcttgtctc gaaaggtcaa
     6661 tgcaggagag gtgcggattc tccttgcttc aactgaaaaa ggaggaacag gtttaaatgt
     6721 tcagagcaag atgaaagcag ttcatcatct ggatgtcccg tggagaccaa gtgacattca
     6781 gcaacgcaat ggccgtatta tccgacaggg aaatgaaaac aaggaagtgg atatttacca
     6841 ctacattaca aaaggttcat ttgataatta tctatgggca actcaggaga acaaactccg
     6901 ttatattaag cagattatga cttctaagga gccgattcgt gctgcagaag acattgatga
     6961 acagactatg acagcttctg attttaaggc actagcaaca ggtaatcctt atctcaaata
     7021 taagatggaa ttggagaatg atctaaccct attagaaaat caaagacgcg cctttcaacg
     7081 cagcaaggat cactatcgtc atacaatctc ttactgtgaa gaaaatatgc ccattcttga
     7141 gaaacgatta agcaagtatg aaggcgacat tcaacagtct gaaatgtcga aagaccaggc
     7201 attttctatg acggtaggta agcaagcttt tgagcaacga gctgaagcag gtgaatctct
     7261 acatcgcctc atccgtcata accaatctga cagcaaagaa ttccgtaccc tagcaagcta
     7321 tcgaggattt gacattaaaa tgcttagtct tccaacaaac caacctcttc ctgaaacctt
     7381 ctctgttaag attgtaggag aaaatcagta ttctgtcagt ttagatttgt attctccttt
     7441 ggggacaatt caaaggcttc agcatacgat tgatcacatt aaagaggacc aagtgaaaac
     7501 tcagaattta ttggatgaat tacaggataa attgactact gctaaagttg aaattgagaa
     7561 aaattttcca aaggaagagg attatcaaac taaaaaggcc gaatacgatg tactcgcgcc
     7621 attgattgaa acagaaacgg atttagatat tattgatcag gccttaagac aattccacga
     7681 aaaaggaaaa gaaaagcaag aacaactttc ttttgaatta gattaaaaaa tagatacaaa
     7741 tagcggacaa gaaaagaaaa gcgcggtaaa ataaagatag aaagaaacga ggtaacgatt
     7801 atgatggaag atacctatta tcaattagaa gaggctttgg tacagggatt tcaaacacct
     7861 gaagaatacc aagcctacaa ggagttaaag gaacattatg aggaagtgac aggggattac
     7921 agtttttcca aacgagaact tactagccaa ttggaaatcg ctcttcagaa tcatcgaggt
     7981 gtggactttg aagattatga gaaagaggac tatctggaat tggttcaaaa actagaagag
     8041 tttgattcct ctcttgccac ccattatcgt cagttaattg attaggaagg aacaagtcat
     8101 gaatgattta ctccttatcc cagtaatttt tttagcagta ggaggaattc tcattctttt
     8161 atggaggatc ttccttattg ccagtggact tttccttatt ggttttgtca gttttcttat
     8221 tttcgtggag gtctatggaa tttatttgtt ctttacagag accgaacttt acacggaaga
     8281 tttagcacag aatggattat ttggttttac gactttcttt atcatcttta accttacttt
     8341 gcttgtcctg gcaggctggg cagggtataa gcggaagagg cataataacg ttttagcaaa
     8401 tcaacgcgat ttttggtgag ttcttcattt tcggtatctt cctatctatt atttttatgc
     8461 ttggtacagt attagttatc tactataaat ctctgaggat tatgaagact gcaaacgctt
     8521 tatcctcttg taaaatatag gtctagatca aaagcaaatc ttgggagaat atcctatatc
     8581 caaaagttac tagatataaa gaattgttga tgaggattcg atggtggcgg gttaaccaat
     8641 cactactagc tcagcttaac taaaatataa aaaatagtct gggataatat tcccttatct
     8701 ccagaaaaag aaactgcaaa tccattgcta tttaacatgg ttgcgatagt cttggtaaaa
     8761 tagaattgcc caataaacca tttacagatg ccgctttgat gccgataaaa gaaaaccata
     8821 tgaagagtgg taaacttaag gctaaaacta aaaatttttg tactttagtt aactcttgat
     8881 aaaactgggt taagtcttaa tatatactct ttaaaaaatg ta
//
LOCUS       DAVMEI010000078         8822 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVMEI010000078 DAVMEI010000000
VERSION     DAVMEI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.078"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            408..3683
                     /gene="rexB"
                     /locus_tag="VYQ24_001327"
     CDS             408..3683
                     /gene="rexB"
                     /locus_tag="VYQ24_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1968461.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3680..7330
                     /gene="addA"
                     /locus_tag="VYQ24_001328"
     CDS             3680..7330
                     /gene="addA"
                     /locus_tag="VYQ24_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1968462.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7545..8723
                     /locus_tag="VYQ24_001329"
     CDS             7545..8723
                     /locus_tag="VYQ24_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968463.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLLLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2720 a         1853 c         1879 g         2370 t
ORIGIN      
        1 cacggtgtac tattatagtt tcaatttact atagttcaat cattctatca agaatattga
       61 atatataaag gcacgtaata ttaaaacttc ttctgatatc gaattagagt ccgatgttga
      121 tggcgataaa tcagacaatc taccagtcga cattaagata ctgggacaac acattgaagt
      181 gtattccgta cctaagaaac aatcataaaa ctcaaagcct tgttaccgag gcttttttat
      241 tatgtgattt tcagtagcca gacttattct atctaataca agtaaagcta agtatttaag
      301 atttagcaga tgatgaagaa aagaaaggat ggcctaaata ttttaacaga tttacgaggc
      361 taatcatggt ataatatagg gtgatgaaaa attccaacga ggctgagatg aaattacttt
      421 atactgatat tcggacttct ttgacagaaa ttctaacaag agaggcagaa gagctagttg
      481 cagctggcaa gcgggtcttc tacattgccc ccaactctct ttcttttgaa aaggaacgcg
      541 ccgtgctgga atgcttgtcc cagcaggctt ctttttcgat taccgtcacg cgctttgctc
      601 aaatggctcg ttatctggtc ttgaatgatt taccagctaa aactactctt gatgatatcg
      661 gtcttgggtt ggccttttac aaatgccttg ccgaactcga tcccaaggac ttgcgtgttt
      721 atggcgctat taagcaggat cctcaattga tccagcagtt aattgagctt taccatgaga
      781 tgaccaaatc ccagatgagt tttttggact tggagaattt aacagatgag gataagaggg
      841 cggatttact cttgattttt gagaaagtaa cagcctatct taatcaaggt cagttagccc
      901 agggaagtca gttgtcccat ttgattgagg ctattgagaa tgacaaggta agtagtgatt
      961 ttaatcaaat caccttggtc attgacggct ttactcgttt ttctgctgag gaagagcggg
     1021 ttgtggactt acttcacggc aaaggtgttg agattgttat cggggcttat gctagtaaga
     1081 aagcctatac cagtcctttt agcgagggca atctctacca agccagcgta aaatttctcc
     1141 atcatctggc ttctaaatac caaacgcctg ctcaggactg ttctcaaact catgagaaga
     1201 tggatagttt tgacaaggcc tctcgtttgt tggagtcttc ttatgacttt tcagaactcg
     1261 ctttggatgt cgatgagaaa gaccgtgaaa atttacaaat ctggtcttgt ttgacgcaaa
     1321 aggaggagtt ggagctagta gcccgtagta ttcgtcagaa attacatgag aactcagacc
     1381 tgagctacaa gcattttcgt attctcttgg gggatgtagc ttcttaccag ttatctctca
     1441 aaaccatttt tgaccagtat cagattcctt tttatcttgg tagaagcgaa gccatggctc
     1501 atcatccctt gactcagttt gtcgagtcta ttttagcttt aaaacgttac cgttttcgtc
     1561 aggaggattt gattaatctt cttagaactg atttgtatac tgacctcagt cagtctgata
     1621 ttgatgcttt tgagcaatat atccgctatc ttggtatcaa tggcttgcca gcctttcagc
     1681 aaatcttcac caaatcccac catggaaaat ttaatcttga gcgtttgaat gtcctccgcc
     1741 tgagaatttt agcacctctt gaaaccctct ttgccagccg aaaacaaaag gctgaaaacc
     1801 tcctacaaaa atggagtgtc tttctaaaag aaggagctgt gaccaagcag ttacaagatt
     1861 tgacaaccac tttggaagct gtagaacagg aaagacaagc cgaagtttgg aaggctttct
     1921 gccatgtttt agaacaattt gcgactgttt ttgctggttc acaggttagt ctggaagact
     1981 tcctagcctt gctccattct ggaatgagtt tgtcccaata ccgtaccatt ccagcaacag
     2041 tggacactgt tctggtgcag agttacgatt tgattgcacc attgactgct gactttgtct
     2101 atgctattgg actaactcag gacaatttac caaaaatttc tcaaaacacc agtcttctga
     2161 cagatgaaga aaggcaaaac ctaaaccaag cgaccgaaga aggcgttcaa ttactgattg
     2221 ccagcagtga aaatctcaag aaaaatcgct acactatgct ttccttggtc aattctgctc
     2281 gtaagcagtt gttcttgtcg gctccaagcc tttttaacga aagtgaaagt aaggaatctg
     2341 cctatcttca agagttgatc cattttggat ttaggcggag agagaagagg atgaatcaca
     2401 aaggactgtc taaggaggat atggggtcct atcacagtct tttgtctagt ctggttgcct
     2461 atcaccagca gggtgagatg agcgatactg agcaagattt gacttttgtc aaggttctgt
     2521 cgcgtgtcat aggtaaaaaa ctagatctgc aaggtctgga aaatccagct atcccaacca
     2581 gtccaagcag caagacctta gccaaggaca ccttgcaagc tctctatcct gccaaacagg
     2641 agttttacct gtctacgtcg ggtttgacag agttttatcg caatgaatac agttatttcc
     2701 tacgctacgt tttaggcttg caggaggaat tacgtttgcg tcctgatgcc cgtagtcacg
     2761 ggaatttctt gcatcgtatc tttgaacgcg ccttacagtt gcctaatgaa gattcctttg
     2821 accaacgtct agaacaagct attcaagaaa ccagtcaaga acgcgaattt gaagctattt
     2881 atcaagaaag tttggaagcc cagtttacca aggaagtttt gcttgatgtt gcacggacga
     2941 ctggacatat tctccgacac aatccagcca tcgaaaccat caaagaagaa gcaaattttg
     3001 gtggaaaaga ccaagccttt attcaattag acaatggacg cagtgtcttt gtacgaggca
     3061 aggtggaccg gattgaccgt ttgaaagcta atggagcgat aggagtagta gactacaaat
     3121 ccagtctgac tcagttccag tttcctcatt tctttaatgg gctcaattct cagttaccaa
     3181 cctatcttgc tgccctaaaa agagaaggga agcagaactt tttcggcgcc atgtacttgg
     3241 aaatggctga acctgtccaa tctctgatgg cggtaaaaag tctggcagga gcagtggtag
     3301 aagccagcaa atctatgaaa taccaagggc tcttcttgga aaaagaaagc agttatttag
     3361 gcgaatttta taacaaaaac aaggctaatc aactgacaga tgaggaattt cagctcctac
     3421 tggactacaa tgcctatctt tacaagaaag ctgctgagaa gattttagca ggccggttcg
     3481 ccatcaatcc ttatactgaa aatggcagaa gcattgcccc atacgtccag caacatcagg
     3541 ctattacagg ctttgaagcc aattaccatc tgggccaagc ccgtttccta gaaaaattgg
     3601 acctagctga tggcaagcgt ctggtcggag aaaaactcaa gcaagcttgg tttgaaaaaa
     3661 taagagagga gttaaatcga tgaagcttat tcccttttta agtgaggagg agattcaaaa
     3721 actgcaagaa gcagaagcaa attcgagcaa ggaacagaag aaaactgccg aacaaatcga
     3781 agctatctac acttctggtc agaatatcct ggtctcagca tcggctggtt ctggaaagac
     3841 ctttgtcatg gcagagcgca ttctggacca attggcgcgt ggtgtcgaaa tttctcaact
     3901 ctttatctca acctttaccg tcaaggctgc aactgaactt aaagaacgtt tagagaaaaa
     3961 aatcagcaag aaaatccaag aaacagatga tgtcgatctc aaacaacact tgggtcgcca
     4021 gttggcagac ctacccaacg ctgccatcgg aaccatggac tctttcacac aaaaattcct
     4081 tggcaaacat ggttatctgc ttgatattgc acctaatttc cgtattttac aaaaccaaag
     4141 cgagcaactt cttctcgaaa acgaagtctt tcatgaggtc tttgaagcgc attaccaagg
     4201 taaacagaaa gagagcttta gtcatttgct gaaaaacttt gctgggcgtg gcaaggacga
     4261 acggggtctg cggcagcaag tctataaaat ctatgacttc ctccaatcca ccagtaatcc
     4321 tcaaaagtgg ctgagtgaat ctttcctcaa aggctttgag aaagctgatt ttaccagtga
     4381 aaaagaaaaa ctgactgagc aaatcaaaca agccctctgg gatttggaaa gctttttccg
     4441 ttaccatctg gataacgatg ccaaggagtt tgcaaaggct gcctatttag aaaatgttca
     4501 gttaattctg gatgaaattg gctccctaaa tcaggagttc gatagtcagg cttatcaggc
     4561 agtacttgcg cgtgttgtcg ccatctctaa ggagaaaaac ggtcgtgctc tgactaatgc
     4621 cagccgtaag gctgatttga agcccctggc tgatgcctac aacgaagaga gaaagaccca
     4681 gtttactaaa ctaggacaat tatcagacca gatagcgatt ctcgactatc aagaacgtta
     4741 tcatggagac acttggaaac tagctaaaac cttccaatct ttcatgagcg attttgtaga
     4801 ggcttatcgt cagagaaaac gacaggaaaa tgcctttgaa ttcgctgata tcagccatta
     4861 caccattgag attttagaaa atttcccaca agttcgtgag tcttatcagg agcgcttcca
     4921 tgaagtcatg gtcgatgagt atcaggatac caaccatatt caagaacgga tgctggaatt
     4981 gttgtctaat ggccacaatc gctttatggt gggagatatc aagcaatcca tctatcgttt
     5041 ccgtcaggca gacccgcaga ttttcaatga gaaattccaa cgctatgcgc aaaatcccca
     5101 agaaggcaag ctcattctcc tcaaggaaaa tttccgtagt agttcagaag tgctgtcagc
     5161 aaccaatgat gtctttgaac gtctcatgga ccaagaggtc ggcgaaatca actatgataa
     5221 caagcaccag cttgtttttg ccaataccaa actgactccc aatccagaca acaaggcaga
     5281 atttctcctc tacgacaagg acgatacagg tgaggaagaa gagagtcaaa cagaaacgaa
     5341 actaacaggc gaaatgcgct tagttatcaa ggagattctg aaacttcatc aagaaaaagg
     5401 tgttgccttt aaggaaattg cccttctgac ctccagccgc agtcgtaatg accagattct
     5461 cctcgccctg tctgagtacg ggattcctgt caaaactgac ggagagcaaa acaattatct
     5521 ccaatcccta gaagtgcaag tcatgctaga cactcttcgt gtcattcaca atcccctgca
     5581 agactatgcc ttggttgccc ttatgaagtc tccaatgttt ggttttgatg aggatgagct
     5641 agcacgtttg tcccttcaga aagcagagga taaagtccac gaaaatctct atgagaaact
     5701 ggtcaatgca caaaaaatgg caagtagtca aaaaggcttg attcacacag ctctagctga
     5761 aaaactaaag caattcatgg atatcctagc ttcttggcgc ttgtatgcca aaacccactc
     5821 tctctatgac ttgatttgga agatttacaa cgaccgtttt tattatgact atgttggggc
     5881 tttgccgaat ggtcctgcta ggcaggccaa tctctatgcc ctagcactgc gtgctgatca
     5941 atttgaaaag agcaatttca aaggtttgtc gcgttttatt cgtatgattg accaagtctt
     6001 agaagcccag cacgatttgg caagcgtggc cgtcgcaccg ccaaaagatg cagtagagct
     6061 catgaccatc cacaagagta aagggctgga gtttccttac gtctttatcc tcaatatgga
     6121 tcaagatttc aacaagcaag actctatgtc agaagtcatt ctcagtcgtc agaatggtct
     6181 tggtgtcaaa tatattgcca agatggagac aggggcagta gaagaccact atcctaaaac
     6241 catcaaactc tccattccta gtctgaccta taggcagaac gaagaggaat tacagctagc
     6301 aagctattct gagcagatgc gtttgctgta tgttgctatg acgcgggctg agaaaaagct
     6361 ctatcttgtc ggcaagggtt ctcgtgaaaa gctggaagtc aagcaatacc cagcagccaa
     6421 aaatgggaaa ctaaatagca atactagact gcaagcacgg aatttccaag attggctttg
     6481 ggctatcagt aaagtgttta ctaaggacaa gctcaacttt agttatcgtt ttattggcga
     6541 agatcagttg accagagaag ctatcggaga gttggaaacc aagagtcctc tccaagatag
     6601 ctcccaagca gacaatcgtc agtcagatac catcaaagaa gctctggaaa tgctgaagga
     6661 ggtggaagtt tataatactc ttcaccgcgc agctattgaa cttcctagtg ttcaaacccc
     6721 aagtcaaatc aagaaattct acgaaccagt tatggatatg gaaggtgtcg agattgctgg
     6781 tcaaggtcag tcagtaggca agaaaatcag cttcgatttg ccagattttt caaccaaaga
     6841 aaaggtaact ggagctgaga ttggtagtgc tactcacgaa ctcatgcaga gaattgacct
     6901 cagccagcaa ctaacccttg ctagcctaac agaaacactc aaacaagttc aaactagcca
     6961 agctgtcaga gacaagatca atcttgataa aattcttgct ttctttgaca cagtactcgg
     7021 tcaggaaatt cttgctaata ccgaccatct ctatcgcgag caacctttct ccatgctcaa
     7081 acgagaccaa aagagtcagg aagactttgt tgtccgtggt atccttgatg gctatctgct
     7141 ttacgaaaac aaaattgttc tgttcgacta caagacagac cgctatgatg aaccaagtca
     7201 actcgtagac cgctatcgtg gtcagttagc tctatacgaa gaggctttat cacgagccta
     7261 ttcgattgaa aatattgaaa aatacttgat tttactcggt aaagacgagg ttcaagttgt
     7321 aaaagtataa cttatactct ttgaaaatct cttcaaacca cgtcagcttc gccttgctgt
     7381 atatatggtt actgacttcg tcagttctat ccacaacctc aaaacagtgt tttgagctga
     7441 cttcgtcagt tctatctaca acctcaaaac agtgttttga gctgattgcg gctagctttc
     7501 tagtttgctc tttgattttt attgagtatt agaaaggaga cctcatgaca cttccagtta
     7561 gaaaatccct gcacgatgca gttttacagg cttcgaaagc ggatacttgg gaacaagcta
     7621 ccaaggaatg gaatgaagtt tccttgattt ttaacggtat cggccgtagc aattgtgtct
     7681 gtggaaatgc catcaaatac gcttatgaac tctttaacgg tgtcacaggc caacgcctct
     7741 ttccaattgg tagcgactgt gttcgtcatt ttcatcgatt gtcccttgac cagcaattgg
     7801 aagaggaaga aaaactgctc agaaaggttg aaaatctaac cagaaaagct cagaaaaagg
     7861 aaaaaatcaa ggtcaataaa agtgactttg acgagcgact tctaaaatgg ctctgggaaa
     7921 aaggtgtttt caaacccaat cgtggcaatc aatttgcgcc tgagagagac taccagcttt
     7981 tactagaagt ctttcaggga ggaagttgga ccaaggcgga accaaagaaa aaggctcgga
     8041 tggaagaagt ccttgaaaag tgtatcaaac cctttttact tgggaaatcg gatgaccaac
     8101 tctaccttgt caagctaggc aaggagaaaa ttgactacga acaagaactc cgtatccagg
     8161 cagagaaaga acgcaagaag agggataaaa tcgccaagca atatgcagac aatctggttc
     8221 tagccatggg acccgcagag cgtgcttatc aagattactt tggctttaca gagaccctga
     8281 ctcaagaaga acgcaagtgg gaaaaaattc tttttggtaa aaatagagca gaacgggcta
     8341 tcaaggccaa acaataccaa aaagagctgg aaaaagacca acgaattgcc agtcaggatc
     8401 caattgaaag aaaacagaag cagacttggc tcctcaattc ctattttcgt gagcttcctg
     8461 aagaaaaagc tcgattttct aggctcttat tagaatatcg aaaaagtggt gaagtaccct
     8521 tttcaactga atacctgtcc gatcatctca tcgacttttt ctataaaatg aaagcctttg
     8581 aatttgaaat cgcaccagaa caagtccgag attttctaaa aaaaagcctg caggaagacc
     8641 atcgctcatc agcacaagga agctggatag aaggaattct cctcaactgc ctcaaaccat
     8701 ttttagaacg attagtgata tgagtaacac ctaccgtgta aatttcatgg taggtgttat
     8761 tgtaccccca aataatataa aacaaagtat gaaagcgcat ctcaatttta agactttttt
     8821 gt
//
LOCUS       DAVMEI010000079         8593 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVMEI010000079 DAVMEI010000000
VERSION     DAVMEI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.079"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            90..494
                     /locus_tag="VYQ24_001330"
     CDS             90..494
                     /locus_tag="VYQ24_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968464.1"
                     /translation="MKILKRYILELCFILSFALPFTKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFICSILIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            710..1291
                     /locus_tag="VYQ24_001331"
     CDS             710..1291
                     /locus_tag="VYQ24_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1968465.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            1288..2565
                     /locus_tag="VYQ24_001332"
     CDS             1288..2565
                     /locus_tag="VYQ24_001332"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1968466.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYCPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            2583..3245
                     /gene="deoC"
                     /locus_tag="VYQ24_001333"
     CDS             2583..3245
                     /gene="deoC"
                     /locus_tag="VYQ24_001333"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1968467.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            3232..3621
                     /locus_tag="VYQ24_001334"
     CDS             3232..3621
                     /locus_tag="VYQ24_001334"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1968468.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3711..4763
                     /locus_tag="VYQ24_001335"
     CDS             3711..4763
                     /locus_tag="VYQ24_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1968469.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4907..6442
                     /locus_tag="VYQ24_001336"
     CDS             4907..6442
                     /locus_tag="VYQ24_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968470.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            6435..7493
                     /locus_tag="VYQ24_001337"
     CDS             6435..7493
                     /locus_tag="VYQ24_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968471.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KEFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            7496..8452
                     /locus_tag="VYQ24_001338"
     CDS             7496..8452
                     /locus_tag="VYQ24_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968472.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2438 a         1518 c         2036 g         2601 t
ORIGIN      
        1 caatttattg atgaaatagg agatagattc tactgattta taagcttata aagggggctt
       61 tttagttcac aacttatagg aggtgtacta tgaaaatctt aaaacgttac atattggaac
      121 tctgttttat tttaagtttt gctttacctt ttacaaaagg agccaatgca gataatggta
      181 gatgctttgt ggaaacctat tacggtttta cttttttgat ggaacatgct attgtaacag
      241 ctgtctttat ctgttcgatc ttaattgctt tcttactaaa aaaacgatgg gcgaaatgga
      301 ttgctgctgg tagttattgc tttttagtcc tatggattgc gacagagggt tatttcttcc
      361 gcatgtcact agaagatttg atacgacttt ggacaagttt ggaaatcctg acacaaacgt
      421 atcagttggg cttttatcta aacattttgt tgggaatttt gttgataata aagtatttta
      481 aggttaagca atagaaataa taatctgcct gattatagat gataaacttt ttgtttttat
      541 ctggactgtg atatttatag ttctaaatgg tttattattt ataagtgttg aaagagattg
      601 aagaaaatgg cttcgtgact cgctttcttc ggtctttttc gttttattat caatttgtaa
      661 acgatttaca atttgagtca aattatggta aaatagtgtc atgagtaaaa tgtattatgc
      721 agaaaatcct gacgctgctc acgacattca tgagttgaga gtagagttgt tgggacaaaa
      781 aatgaccttt ttaacggatg cgggtgtttt tagcaagaaa atagttgact ttggaagtca
      841 actcttgctc aagtgtcttg aggtcaacca aggagaaact gtccttgatg taggctgtgg
      901 ttacggacct ttgggtttgt ccttagctaa ggcttatgga gttcaggcta ctatggttga
      961 tatcaacact cgtgccttgg atttagctcg gagaaatgct gaaaaaaata atgcaaaagc
     1021 gacgattttc cagtccaaca tctatgaaca agttgaggga cattttgatc atgtcatttc
     1081 caatcctcct atccgtgcgg gcaagcaagt cgttcatgaa atcattgaga aaagtaaaga
     1141 tttcttggaa actggtgggg atttgacaat cgtcatccag aaaaaacaag gggctccaag
     1201 tgctaagtcc aagatggagg atgtgtttgg taattgtgaa atccttaaaa aagacaaagg
     1261 atactatatc cttagaagtg tgaaagaatg agagcagttg atttaataca aaaaaaacga
     1321 gacggtcaag aactgacttc aagtgaaatt gaatggctga tagaaggcta tgtgtcagga
     1381 actgttcctg attatcagat gtctgccttt gctatggctg tttattttaa aggaatgacg
     1441 actcgtgaaa tttctgattt aacgatgaat atggttaaga ctgggcaaga gtttgacctg
     1501 tcagccattg atggtgtgaa agttgacaag cattctactg gtggtgtcgg cgataaggta
     1561 accttgattt tagctcctct tgttgctagc ttcggtgtgc ctgtagcaaa aatgagtggt
     1621 cgtggtcttg gccatacagg cggaacaatt gataaattgg agtccattaa gggctatcaa
     1681 gtagaacgta gtcaagagga ttttattcgt caggttcagg atattggagt atctgtcatt
     1741 gggcaatcag accagctggt caaagcagat aagcttctct atgcccttcg tgatgtgacc
     1801 gcaactgtcg acacgattcc tttgattgcg agttcggtga tgagcaagaa aattgcggca
     1861 ggggcggatg ctattttgct agacgtgact gtcggtgagg gtgccttcat gaagacggtt
     1921 gatgaggcgc gtgagttggc tcaaactatg gtagatcttg gtaaggttgt tggacggaag
     1981 acagtagcag tgattacgga tatgagccag cctttgggac gagcgattgg aaatcgtctg
     2041 gaaatccttg aagcattgga gattttgcaa ggtcaaggac gtcaggatat tacccacttt
     2101 atttgtgaat tggctcaaat tatgcttggt ttggcaaatg ttaacaaaac tgttgaagaa
     2161 gttcgccaac atcttgagaa tggtcaagca ctggctaagt ttgaggaaat ggtgcaagcc
     2221 caaggtggag acttggaaga cctctattgt cctgtaaatg tagctcatgt ggtggaaatc
     2281 cctgctcagg aaacgggtgt catttcagct cttccagcta tggactttgg tctttatgcc
     2341 atgaggctgg gtgctggtcg tgcagttaag tctgatgcct tggactatga aacaggaatt
     2401 gtttttgaaa agaagattgg agactccgtt caaaagggtg aaattgttgc aaaagtatac
     2461 acaaatgaaa aaattccacc tcaactagtt acagatttcc aaaaatgtgt taaaataagt
     2521 gatgaagtga aaaaaatccg tgaaattgta gaaatcatct cctaaatgga gggaaaaccg
     2581 aaatgaaatt aaataaatat atagatcata cgcttttaaa acaagatgca aagaaaaaac
     2641 aaattgatag tttgttgtct gaggctagag agtatgactt tgccagtgtt tgcgttaatc
     2701 cgacctgggt tgaacatgct aaaaaaggac ttgaaggcac agatgttaag gtttgcacag
     2761 tagtaggttt ccctttggga gcaacaactt cagccgtgaa agcatttgag acaaaagaag
     2821 ctatccaaaa tggtgcagat gagattgata tggtgatcaa tgttggagct ctcaaatcag
     2881 gtaatttagc cttggttgag tcagatattc gcgcagtagt ggaagcaagt ggtgataagt
     2941 tagtgaaagt cattattgaa gcttgccttc tgacagacca agaaaaaatt gttgtttgcc
     3001 aattggccca aaaagctggg gctgactttg ttaaaacatc tactggtttt tcaactggtg
     3061 gtgctacgat agcagatgtt acattaatgc gtgaaacagt tggatctgat atgggagtca
     3121 aggctgctgg tggagctcgt tcttatgcag atgctcttgc ctttgtcgaa gcaggtgcga
     3181 cccgtatcgg aacgtcagct ggggtagcta ttttaaaagg agaattggca gatggcgact
     3241 actgagttga ttgaactggc aattgaaacc agcaaacatg cctatgtccc ctattctcac
     3301 tttcctatcg gagcagtttt agtagccaaa gacggaagtg tttacacggg agtgaatatc
     3361 gaaaatgcta gctatccttt gaccaattgc ggtgagagaa cagctgtttt taaggctata
     3421 tctgaaggcc aaagagaatt ttcagaattg attgtctatg gtcaaactga aaaaccgatc
     3481 tcgccatgtg gtgcttgtcg ccaagtaatg gtcgaatttt ttgaacaaga tttaaaagtg
     3541 accttagtcg caaaagataa atcgacggtc gagatgacgg tcggggaatt acttccatac
     3601 tcttttacag acttaaacta gtctgagtcg ctctctgagt ggcacggtcc ttgtggccaa
     3661 ccaatccata cttgcaacat cgttgcacat cttatttagg aggttcagta atgaacaaga
     3721 aacaatggct aggtcttggc ctagttgcag tggcagcagt tggacttgct gcatgtggta
     3781 accgctcttc tcgtaacgca gcttcatctt ctgatgtgaa gacaaaagca gcaatcgtca
     3841 ctgatactgg tggtgttgat gacaaatcat tcaaccaatc agcttgggaa ggtttgcagg
     3901 cttggggtaa agaacacaat ctttcaaaag ataacggttt cacttacttc caatcaacaa
     3961 gtgaagctga ctacgctaac aacttgcaac aagcggctgg aagttacaac ctaatcttcg
     4021 gtgttggttt tgcccttcac aatgcagttg aagaagcagc taaagaacac actgacttga
     4081 actatgtctt gattgatgat gtgattaaag accaaaagaa tgttgcgagc gtaactttcg
     4141 ctgataatga gtcaggttac cttgcaggtg tggctgcagc aaaaacaact aagacaaaac
     4201 aagttggttt tgtaggtggt atcgaatctg aagttatctc tcgttttgaa gcaggattca
     4261 aggctggtgt tgcgtcagta gacccatcta tcaaagtcca agttgactac gctggttcat
     4321 ttggtgatgc ggctaaaggt aaaacaattg cagccgcaca atacgcagcc ggtgcagata
     4381 ttgtttacca agtagctggt ggtacaggtg caggtgtctt tgcagaggca aaatctctca
     4441 acgaaagccg tcctgaaaat gaaaaagttt gggttatcgg tgttgatcgt gaccaagaag
     4501 cagaaggtaa atacacttct aaagatggca aagaatcaaa ctttgttctt gtatctactt
     4561 tgaaacaagt tggtacaact gtaaaagata tttctaacaa ggcagaaaaa ggagaattcc
     4621 ctggcggtca agtgatcgtt tactcattga aggataaagg ggttgacttg gcagtaacaa
     4681 acctttcaga agaaggtaaa aaagctgtcg aagatgcaaa agctaaaatc cttgatggaa
     4741 gcgtaaaagt tcctgaaaaa taatggatgg aagtcatttc ttaggaagcg gccaagggcc
     4801 gcttttttaa gagttgagac aaagtcattt tgtctcagta aagcttgcta ctagacaaac
     4861 tagcaagttt tattgaaata aaataagact ctgaaaggaa gagcacatgg cacacgaaaa
     4921 tgtcattgag atgcgtgata ttaccaaggt gtttggtgga tttgttgcca acgacaaaat
     4981 caacttgcac ctacgaaaag gtgaaattca tgcactttta ggagaaaatg gggctggtaa
     5041 gtccacgcta atgaacatgt tagcaggcct tcttgaacca actagtggtg aaatcgcggt
     5101 caacggtcaa gttgtcaatc tcgactcccc atctaaagca gctagcttgg gaatcgggat
     5161 ggttcaccag cactttatgt tggttgaagc cttcacagtg gctgaaaaca tcattttagg
     5221 tagtgaattg actaaaaatg gtgtgctaga tatcgctgga gctagcaaag aaatcaaggc
     5281 tctttctgaa cgttatggct tagctgttga cccttctgcc aaggtagcag atatctcagt
     5341 tggagcccaa caacgtgtag aaattttaaa aacactttat aggggggctg atatccttat
     5401 ctttgacgaa ccaacggctg ttttgactcc atcagaaatt gatgagttga tggctattat
     5461 gaaaaatctt gtcaaagaag gaaaatcaat tatcttgatt acccacaaat tggatgaaat
     5521 tcgcgcagtt tctgaccgtg ttacagttat ccgtcgtggg aaatcaattg aaaccgttga
     5581 aattgcaggg gctaccaatg ctgatttggc ggaaatgatg gtaggacgtt ctgtttcctt
     5641 taaaacagag aagcaagcct ctaaaccaaa agaagtggtt ttgtctatca aagatttggt
     5701 ggtcaatgaa aaccgtggtg ttccagctgt taaaaatctg tccttggatg ttcgtgctgg
     5761 agagattgtt ggtattgcgg ggattgatgg aaatggtcag tctgaactga ttcaagccat
     5821 tacaggtctt cgtaaggttg aatctggtag cattgagcta aaaggagatt caattgtagg
     5881 cttgcaccca cgtcagatta cagaactaag tgttgggcac gttccagaag accgtcaccg
     5941 tgatggcttg attttggaaa tgatgatatc tgaaaatatt gcccttcaaa cctactataa
     6001 agaaccacat agtaaaaatg gaattttgaa ttattcaaat attacttctt atgctaaaaa
     6061 gctgatggaa gagtttgatg ttcgcgctgc cagtgaatta gttcctgcag ctgcactctc
     6121 aggaggaaat caacaaaaag caattattgc tcgtgaaatt gatcgagatc ctgatctcct
     6181 tatcgttagc cagccaactc gtgggttgga tgtcggtgcc attgagtata tccacaaacg
     6241 cttgattgaa gagcgtgata atggcaaggc tgtccttgtt gtcagctttg aattggatga
     6301 gattttaaac gtctcagacc gtattgccgt tatccacgat ggtaagattc aaggtattgt
     6361 atcaccagaa acaaccaata aacaagaact tggtgtcttg atggctggtg gaaacttggg
     6421 aaaggagaag agtgatgtct aaaaaattac aacaaatttc ggttcccttg atttctgtat
     6481 ttctaggaat tttactcgga gccattgtca tgtggatctt cggttatgat gctatttggg
     6541 gctacgaaga attgttctat acagcctttg gcagtctgcg tgggattgga gaaatcttcc
     6601 gtgctatggg tcctctggtc ttgattggtc ttggttttgc ggttgccagt cgagctggtt
     6661 tctttaacgt cggacttcct ggtcaggctt tggcaggttg gattctcagt ggttggtttg
     6721 cgctgtcgca tccagatatg ccccgtccct tgatgattct agcaaccatc gtgattgcct
     6781 tgattgctgg tgggattgtc ggagcgattc caggtattct tagggcctat ctagggacgt
     6841 cagaggttat tgtaaccatc atgatgaact acattgtctt gtatgtaggg aatgccttta
     6901 tccatgcttt ccctaaagaa ttcatgcaaa gtacagattc gaccattcgt gttggggcta
     6961 atgcaaccta tcagacacct tggttggctg agttgactgg taactcacgg atgaatattg
     7021 gtattttctt tgccatcatt gccgttgcag ttatttggtt catgctcaag aaaacaactc
     7081 ttggttttga aatccgtgca gttggtctta atccacatgc ttcagaatat gctggtattt
     7141 ctgccaagcg gactattatc ctatctatga ttatttcagg tgccttggca ggtcttggtg
     7201 gagctgttga aggtttggga accttccaga acgtctatgt tcaaggttcg tcattagcta
     7261 tcggatttaa cggaatggcg gttagtttgc ttgcggccaa ctcaccaatt ggtatactct
     7321 ttgcagcctt cctatttggc gttctccaag ttggggctcc tggtatgaat gcggcgcagg
     7381 taccatctga gcttgtcagc attgtaacag cgtctattat cttctttgtc agtgttcatt
     7441 accttatcga acgctttgtc aaaccgaaaa aacaagttaa aggaggtaag taaagatgtc
     7501 tataataacc ttgctccccc tcttggtgtc ttctatgctg atttactcag cacctctcat
     7561 ctttacaagt atcggtggtg ttttctctga acgtggtggt gtggtaaacg ttggccttga
     7621 aggaattatg gttatgggtg ccttttctgg agttgtcttt aaccttgaat ttgcagaaca
     7681 atttggagca gcaactccat ggctatcctt gcttgtagca ggattggttg gtagtgtttt
     7741 ctctatcatc cacgcagcag cgacggttca tttccgtgca gaccatgttg tcagcggtac
     7801 ggtattgaac ttgatggcgc ctgccttggc tgttttctta gttaaagttc tttataacaa
     7861 aggacaaacc gacaacctaa gtcaaacttt tggacgcttt gatttcccag tcttggcaaa
     7921 tatcccagtg attggtgata tcttcttcaa gtcaactagt ctacttggtt atctggcgat
     7981 tgccttctca ttccttgctt ggtttattct ctttaagact caatttggtc ttcgtctccg
     8041 ctctgtcggt gaacatcctc aagcagcgga taccttggga atcaacgtct acaagatgag
     8101 atatttaggg attattattt caggttttct aggtggaatt ggcggagcga tttatgcgca
     8161 atcaatctca gttaacttct cagtgacaac tattgttgga cctggattta tcgcccttgc
     8221 tgcgatgatc tttgggaaat ggaatccaat cggtgctatg ctttctagtc tcttctttgg
     8281 actttcacaa agtttggctg ttatcggttc tcaattgccg ttcctacaag gagtgccagc
     8341 ggtttatctt caaattgcac cttatgtttt gacaattctt gtcttagcag ccttctttgg
     8401 aaaagcagtc gcaccaaaag cagatggtat caactacatc aaatcaaaat aagcatacaa
     8461 aaaaacgtca gtccaatgac tgaactgccc cccaaaagtt agacagaaaa aatctaactt
     8521 ttggggtgtt tttattatga aattgagttg tgatgataaa gttcagatct atgaacttag
     8581 aaaacaagga tat
//
LOCUS       DAVMEI010000080         8560 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVMEI010000080 DAVMEI010000000
VERSION     DAVMEI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.080"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            87..539
                     /locus_tag="VYQ24_001339"
     CDS             87..539
                     /locus_tag="VYQ24_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1968473.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(574..1962)
                     /locus_tag="VYQ24_001340"
     CDS             complement(574..1962)
                     /locus_tag="VYQ24_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1968474.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     SASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            2028..2990
                     /locus_tag="VYQ24_001341"
     CDS             2028..2990
                     /locus_tag="VYQ24_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1968475.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(3196..4206)
                     /gene="ccpA"
                     /locus_tag="VYQ24_001342"
     CDS             complement(3196..4206)
                     /gene="ccpA"
                     /locus_tag="VYQ24_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1968476.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(4631..5230)
                     /locus_tag="VYQ24_001343"
     CDS             complement(4631..5230)
                     /locus_tag="VYQ24_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1968477.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSEKLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEMVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(5232..6329)
                     /locus_tag="VYQ24_001344"
     CDS             complement(5232..6329)
                     /locus_tag="VYQ24_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1968478.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILTVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(6329..7066)
                     /locus_tag="VYQ24_001345"
     CDS             complement(6329..7066)
                     /locus_tag="VYQ24_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968479.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTAIFSFPVVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFSVGHFVRGVDMSLGNWIGAALLLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(7068..7952)
                     /locus_tag="VYQ24_001346"
     CDS             complement(7068..7952)
                     /locus_tag="VYQ24_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968480.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENIIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFILTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(7939..8133)
                     /locus_tag="VYQ24_001347"
     CDS             complement(7939..8133)
                     /locus_tag="VYQ24_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968481.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(8130..8336)
                     /locus_tag="VYQ24_001348"
     CDS             complement(8130..8336)
                     /locus_tag="VYQ24_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968482.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         2602 a         1973 c         1525 g         2460 t
ORIGIN      
        1 attcttttga aaaatacttt tttttgcttt acttctatag tgaaaaggtt tacaattata
       61 gtaagaaaat ttcaggaggt ttcattatgg cacaacgtta ccaaaatatc atggtcgcaa
      121 tcgatggttc taaggaagcg gacttggctt ttgtcaaggg agttcattct gctctacgaa
      181 acgacgctaa actcaccatc gcacatgtca ttgacacacg cgctctccaa agcgtatcca
      241 cctttgatgc tgaagtttac gaagaactcc aagtcgacgc tgaaagtctg atgaaagagt
      301 acgaaaaacg tgctaaagat gctggggtag cagatgttca tatcgtcatt gaaatgggaa
      361 atccaaagac cctgctagca cgtactattc cagatgccga ggaagtggac ctcatcctcg
      421 ttggcgcaac tggtctcaac gcctttgaac gcctcttagt cggctcttca tctgaataca
      481 tactccgcca tgctaaggtc gatttgctgg ttgtgagaga acaagaaaaa accttataat
      541 cacaaagaaa aggagcccct agctcctttt tgtttacgat ttatttctct ctttatggcg
      601 ttcgtaagcc ttgagctggc gctgcagttc ctttttaata gcaggttctg gagcatattt
      661 ttcttcccaa ttatctggtt ttaagatttt atgggtcact ggatcaaaat gagccttgcc
      721 atctggaaaa attttcccca tattggcctg atggacaata tcaaaaatac gttctgggtc
      781 cacccccatc aagacaaaac tgccgtaggt gaagtaaagc gtgtcaatca aggcatccac
      841 ttgccctatc aaatcttgct gagcaggtgt cttcttggct actttacctg ctgccttatc
      901 aagggcctga tgaagttgcg atacagcttg accaaaatct tcttcagaag gactggctga
      961 tcgaacaaac tccaccaatt cttctatttt aaaaccagcc ctatgggttg caccctctaa
     1021 atcccaagct cgaggttctt cttgggttcg ttcatccatc atgtggtgga aggtcttgac
     1081 cttattgaaa tgatagtcac ggctgacaaa aactttttct gaagacaaaa caccaaaatg
     1141 ttccaaggcc ttgtggatcc catcttgttg attgctagtg gtaatgtgct tggcagcttc
     1201 cttgacactg ctactaccat ttcccatagc gaccgacata ccgacgccag ccagcatttc
     1261 aaggtcatta tctgagtcac caaaagccat gacttggttg aggtcaaagc catattcttt
     1321 cccaactcgg cgaatgcctt ctaatttaga atttccctga ttgatgacat ccgatgcaaa
     1381 aggattgcta cgtgtcaatt tcaagtcttc aaaatcagct gccgccttct cagattcttc
     1441 tggtgtcatc agcatcaaaa cttggtagat aggctgattc atcaggtgaa gcaggtcctc
     1501 ttccttttgg ggaacaacct tgctgaccat gcgattaaaa gactgactca ccgtccgagt
     1561 taaaacagag ggaacgaagc gactaattcg ttgggaaaaa gaacccagac caaaggacat
     1621 gattttagaa cccaacatgg catccttggt ccctagagca atctccgtgc cctctttttt
     1681 agcatagcta attagatggc gcaaatgtaa cttggaaata gggctcgtga acaagactct
     1741 gtctttacta aagatatact ggccattata ggttaccgca aaatccagat ccaaatcgtc
     1801 catcaattcc ttaacaaaaa aaggtcctcg ccctgtcgct acgccaacta gtaccccttg
     1861 ttctttgaca atcttaatcg catccttagt ggatttcaaa acactcttgc gattgttgac
     1921 caaggttcca tcgatatcaa aaaaaacagc tttgacttcc atcctatccc aatctcccct
     1981 tttgtgatac aatgattata ccacatttca gaaagagtga gtaaatcatg cctaagaaaa
     2041 tccttgtttt acatacgggt ggaactattt ccatgcaggc cgatgcttct ggcgctgttg
     2101 tgacgagttc agataatccc atgaaccatg tgtccaaccc acttgaagga atccaagtcc
     2161 acgccttgga cttttttaac cttccaagtc cccatatcaa acccaaacat atgctggtcc
     2221 tctaccagaa aattaaagag gaagcagata actacgatgg agtggtgatc acacacggaa
     2281 ccgatacttt agaggaaaca gcctatttcc ttgataccat ggaagttccc catatgccta
     2341 tcgttctaac aggagccatg cgtagctcca atgagctcgg tagtgatggt gtttataatt
     2401 acctaagtgc tttacgagtg gccagcgatg acagggctgc tgacaaagga gttttggtcg
     2461 ttatgaacga tgaaatccac gctgccaagt atgtcaccaa aacacatacg actaatgtca
     2521 gcaccttcca gactccaaca catggccccc tcggtctcat catgaaacag gaaatcctct
     2581 acttcaaaac agctgaacct cgtgttcgct ttgaccttga tcacatacaa ggtttagtcc
     2641 ctatcatctc ggcttatgct ggtatgacag atgagctgat tgatatgctg gatttagaac
     2701 acttggacgg tttgattatc caagccttcg gagctggtaa tattcccaaa gaaacggctc
     2761 aaaaattaga aagccttctg caaaaaggaa tcccagtcgc tctggtatca cgatgcttta
     2821 acggtattgc cgagcctgtt tatgcctacc agggtggggg cgtacagttg caaaaagcag
     2881 gcgttttctt tgttaaagaa ctcaatgccc aaaaagctcg cttgaaactc ctcatcgccc
     2941 tcaatgccgg actaacagga caggctttga aagactatat ggaaggctaa tactcttcga
     3001 aaatctctgc aaaccacgtc acgtcgcctt accgtatgta tggttactga cttcgtcagt
     3061 ttcatccaca acctcaaaac agtgtgttgc gcagccttta accagccgcc tagtccgctc
     3121 tatggtattc attaagtcaa catctcttgt ttaagagcac aaaatcagga aatcttctcg
     3181 attccctgat tttttctatt tacgttttcg tgttgagcta cgttctgtca aaccatgagg
     3241 taagagaact tcacgttctt ccaactcttc cttatgcata atcttggtca acatacgcat
     3301 actaatggca ccaaggtcat aaagaggttg ggcaatcgtt gtcaagtttg gacgggtaaa
     3361 gcgtgagatt tgtgaatcat cactagtaat aatttcaaaa tcttctggca cagaaacacc
     3421 cttatcagcc aaaccgttca agactcctgc tgccaactca tcacctgtca caactgctgc
     3481 agttgcattt gatgaaatca aacgctctgc taaggcgtaa ccatcatcat agctatattt
     3541 agattcaaat accaaaccct cactataagt gattcctgct tttttcaagg tttccttgta
     3601 gccaactaaa cgaaccttac cattgatgtc atccactagc ggaccgctaa cgaaagcaat
     3661 acgctcattt tctttagcaa ggtaactcac tgcatcaatt gttgcttgct tatagtcaat
     3721 attgacactt ggcaactggt gctcaacatc gacagttcct gcgagaacaa tcggagtacg
     3781 tgaacgcgaa aattctgagc gaattttatc tgtcaagtga taccccatat agataatgcc
     3841 atctacctgc tttgaaaaga gggtattgac aacagaaact tctttctcgt tatcttcatc
     3901 gctattagct aggacaatat tgtacttgta catttctgca atatcatcaa tccccttagc
     3961 caaactcgaa aaataaccat tggtaatatt tggaatcacg acaccgacag tggttgtctt
     4021 tttacttgca agaccacgcg caactgcatt tggacgataa tccaaacgat caattacctc
     4081 tagcactttt ttacgggtat tctcttttac atttttattg ccattgacca cacggctgac
     4141 cgtcgccatg gaaacacctg cttcacgagc gacatcataa atggttactg tatcatctgc
     4201 attcattcct tttcctgtcc tttctatctc acacattctt ttacaagtag aggtactgat
     4261 tgaagctcta tatctactta caaaagtgaa gatgtgaaaa tttcgttttc atatttctac
     4321 ttattccatt ctatcactaa ttgtaaacac tttcaagtgt tttttgaaga ttgattgaaa
     4381 aaatttcata gaaaacctag gtttagctcc ttgctaccac cttagactaa acaaaaagga
     4441 ggaaactaag ccctcctaaa gttatagtaa aatgaaataa gaacaggata aatcgatcag
     4501 gacagtcaaa tcgatttcta acaatgtttt agaagtagag gtgtactatt ctagtttcaa
     4561 tctactatag gtattgttcc attcactacc gtcaatttta gcacatagtc ttcatgaaaa
     4621 tattatatca tcataaccaa ccagattctt ttgcgatatt agctgcctct gttcgattac
     4681 cagcatccag ttttgaaaga atattggtga catagtttcg aatagttccg tttgagaggt
     4741 aaagctgatc tgcgatttct tggttggata agccccgggc gattcccttt aagacagcaa
     4801 tctcttgttc tgtcaacgga ttgggacggg tcatcaccat ttccatcaat tcaggcgaat
     4861 actccttgcg tccttcgagg acggtgtgca aggtttgcat gaggtctgca atgcttcgtt
     4921 cctttaatac ataagcatcc actccagcct tgaccgcacg ttcaaaatac ccagcacgct
     4981 tgaaggtcgt caccacaacc acctttgttt caagcttttc tgatcgtatc cactccaaga
     5041 cttcaagacc tgtcttaaca ggcatttcta cgtcaaggat ggcgatatct acagactcct
     5101 tttctaatag ttggattgct tcttgcccat tcttggcttg aaggacagac tccacatctg
     5161 gttgaagcgt gagcaattga cacatggcat ctcgcaacat actttgatct tctgcgacta
     5221 atactttcat cttagtttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt
     5281 ttctgactga ttacacttac ttctcctgaa aatggaaaaa cacgatctcg gactgtatgg
     5341 agctcatctc cttttagaaa agcaaagccg cagccatcat cacttactgt taaaatgagt
     5401 tccttctctg tccgttctaa ttttaagtaa gctttagacg ctttggcatg tttgatgata
     5461 ttggtcacca actcaagcaa aatcatagaa gccattgact ccaattcttg agtaaggcta
     5521 gcagtatcta gttggttatc cgtttccacc tcaattccag caatttctaa catctttttc
     5581 acagtctcta gttcggatgt caaagttcta gacttaagat tttccacgat agttcgaact
     5641 tcattcatgg aatccttgct gatctggtga atttctttta attccttttc cacctgtgga
     5701 taagcctcca tctgaaataa ctgcaaggct agatcagttt taacactaag catagcaaag
     5761 gtatgcccca gactatcatg caaatcttga ccgatacgat tgcgttcatt ttcagcaagc
     5821 aatagattta tctgggcatt ttgcttagcc tgtgcttctt tcaaatcctc cacaattcga
     5881 atccgaacca agccaaaagt cattaaatcg acaaaagcaa gaattacaag tagatagaat
     5941 agaaactcaa cttcgattct ctgaaaaatc aacagttgac ccacaacaag gacttgagca
     6001 agaagaaaag tccagacatg taaagacttt aaaccaccta cgctgaaatg atagcttaag
     6061 agattggata ggaaaaagaa aaaccagata taattaacag ccacaaaggc agtattccca
     6121 actacataag tcagcaggat gccccaatat agccaagata gacgctggct cttagttgtt
     6181 aaaacaccca aatacgccac tacaaataga atatcaatca ataaatgcca ggcagaaagc
     6241 cacccagtca ctacagttag gatggggaaa accataaaaa ttaaactgat ccaaaacata
     6301 taatgtatgc ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa
     6361 tggtcaaacc aaacaaaact actgaaaaaa caagtaaata aactgtggct gatagattga
     6421 tgccaccctc atttaagaag gtcttgagca actccatcaa ctgataggtc gggagacact
     6481 tacctactac ttgcatccag tctggaaata aagagatagg catccagagt ccacctaaaa
     6541 cagccaaccc tagataaaga agattgccca cgacagacat caactgacta gttggtaaga
     6601 gagtcaaggt caaaccaagc gctacgaagg caatacttcc tactatcagc aaaagtgcag
     6661 ccccaatcca atttccaaga gacatgtcca cacctcttac aaaatgccca actgagaaaa
     6721 ccaccaagat tgaaaccaaa taatcaacca gcatacttgt tatctttgat agataatatt
     6781 ctaccatatt tacagggcta tgacgcaatg ttttctgcca gttgttgatc ttgtcggtat
     6841 gtaaaacaac tgggaatgag aagatagctg ttgacatcat ggaaaatgca gtcatggaga
     6901 taagataatc acgcataaaa ttcgcgagtt cacctggtgt gtcctgatag ataccagaaa
     6961 aaaataaata gaaagccgtc ggcatcccta ccgacaatag ataatagatt aattgtcgtt
     7021 tggtcaataa aaattctatc ttactaagtg ctatccatcg tttcatctta gttatctccc
     7081 ttctgcgtct cttcaaagat tgtattcaac aaactacgat tattaacttc gatttcttgc
     7141 atcctacatc ctgcttgggc taatagttcc cagaaagcat ctgcttctcg agtgactact
     7201 tgtaaagaat cttgctttag ggtccagttt tcaaccaaat ttgactgctc tacgacttcc
     7261 ttgtaagcta taggaaggat aaagtgcttt tctatttcct cgctacgcat agctagaggc
     7321 gtcgtatctc gaatcaactc tcccttattt aagagcaaaa tgcggtctgc agtgtgttcc
     7381 acttcctcaa tataatggga cgaatagaga atggtgactc cttgcgcttt taactcctgg
     7441 acaatttccc aaaaacgttg acgagttgag gtatccatgg acgcagtagg ctcatctaaa
     7501 aaaacaatct ttggtcgccc aattaaggtc aagatgaaag aaaagagacg cttttgaccg
     7561 cctgataatt tttctgccaa ctgttctttt tgttgcttga caaactgcaa tagttgatca
     7621 atttcctgat tgctcaaagg atttggatag atacgttgaa agaaagcaat caactcttta
     7681 acttttaatt tctgaacgat tatattttct tgagggaggt aagccctagt atagtctaac
     7741 ttagaactcg tcactggtaa accttgaata gatacttgac cgcttgtgac cagtttatct
     7801 ccaagcaaac aatctaagag tgttgtcttt ccggcaccat ttggaccaat taaggcaaca
     7861 cattcaccat cgttgatttc aaaggagatg ttccgcaaaa tttccttgtc ttttattttc
     7921 ttactcaatt tttcaacttt aaccacagtc atgagactct cctttcaacc actccattcc
     7981 catagggaaa acgacgaaaa tcataaatcc aaaccccaaa gcaccacaaa tgaattggcg
     8041 aagcaaggct tgatcaaacc aatcagtaaa catttccact aaccatacca agagtgacag
     8101 gccgataaag aaatagatga tccctctctt cattcctcaa actccttttt cacatctctg
     8161 actaatttca aaccttctct aacaagccaa gacatgattc caaagccagc aaagaccttc
     8221 caaggaaaat ggtagaaatc ctcatccaat cccgaaaaca tgagataggt cataactcct
     8281 gctactacta aactcattgc aataatcatt ttatttctca tctcttcttc ccccatttca
     8341 tactacaatt atagtctttt aaaatcagag gaggcagaag cttctgtcac tagaaaatat
     8401 gacaaatgtc ataaaaattc tgttcaaaac aagcaagata cactatacaa taaaacacaa
     8461 tcagaaaaat ctaaggcaac ttcctcaaaa gagatatcaa acccaattca caccataatg
     8521 taaactaata cttatttaaa atcaaaaaga gtagaaattt
//
LOCUS       DAVMEI010000081         8208 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVMEI010000081 DAVMEI010000000
VERSION     DAVMEI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.081"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..390
                     /gene="psaA"
                     /locus_tag="VYQ24_001349"
     CDS             <1..390
                     /gene="psaA"
                     /locus_tag="VYQ24_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1968483.1"
                     /translation="DKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWE
                     INTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTDSI
                     AEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            513..1004
                     /gene="tpx"
                     /locus_tag="VYQ24_001350"
     CDS             513..1004
                     /gene="tpx"
                     /locus_tag="VYQ24_001350"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1968484.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(1100..3808)
                     /locus_tag="VYQ24_001351"
     CDS             complement(1100..3808)
                     /locus_tag="VYQ24_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1968485.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(3810..4511)
                     /locus_tag="VYQ24_001352"
     CDS             complement(3810..4511)
                     /locus_tag="VYQ24_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968486.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(4761..7172)
                     /locus_tag="VYQ24_001353"
     CDS             complement(4761..7172)
                     /locus_tag="VYQ24_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1968487.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(7225..7917)
                     /locus_tag="VYQ24_001354"
     CDS             complement(7225..7917)
                     /locus_tag="VYQ24_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1968488.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(7968..>8208)
                     /locus_tag="VYQ24_001355"
     CDS             complement(7968..>8208)
                     /locus_tag="VYQ24_001355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968489.1"
                     /translation="IFDDYFTKAEEGFKGTAMENNDSFTKLKESTQKLFDAQKKRLNN
                     EDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
BASE COUNT         2364 a         1931 c         1545 g         2368 t
ORIGIN      
        1 gacaaacttg ataaagaaag taaggataaa tttaataaga tccctgctga aaagaaactc
       61 attgtaacca gcgaaggagc attcaaatac ttctctaaag cctatggtgt cccaagtgcc
      121 tacatctggg aaatcaatac tgaagaagaa ggaactcctg aacaaatcaa gaccttggtt
      181 gaaaaacttc gccaaacaaa agttccatca ctctttgtag aatcaagtgt ggatgaccgt
      241 ccaatgaaaa ctgtttctca agacacaaac atcccaatct acgcacaaat ctttactgac
      301 tctatcgcag aacaaggtaa agaaggcgac agctactaca gcatgatgaa atacaacctt
      361 gacaagattg ctgaaggatt ggcaaaataa gcctctgaaa aacgtcattc tcatgtgagc
      421 tggcgttttt tctatgcaca catttccggt caaatcattg gaaaattctg actgtttcag
      481 atacaatgga agaaaaaaga ttggagtatc ctatggtaac ttttctcgga aatcctgtga
      541 gctttacagg taaacaacta caagtcggcg acaaggcgct tgatttttct cttactacaa
      601 cagatctttc taaaaaatct ctggctgatt ttgatggcaa gaaaaaagtc ttgagtgtcg
      661 ttccttctat cgatacaggc atctgctcaa ctcaaacacg tcgttttaat gaagaattgg
      721 ctggactgga caacacggtc gtattgactg tttcaatgga cctacctttt gctcaaaaac
      781 gttggtgcgg tgctgaaggc cttgacaatg ccattatgct ttcagactac tttgaccatt
      841 ctttcggacg cgattatgcc ctcttgatca acgaatggca cctattagca cgcgcagtct
      901 ttgtcctcga tactgacaat acgattcgct acgttgaata cgtggataat atcaattctg
      961 agccaaactt cgaagccgca attgcagctg ctaaagccct atagaaaaaa tcctctgtca
     1021 caaagagttc cctgctcttt gaaacggagg atttttgaat attagtaaat agaagttcaa
     1081 tcagctaaaa ataactacct tactctacag atttcagggc ttctaacatg tcaacctttc
     1141 tcagataata attgacgaag aaaccaagca aggtcaaaat gatgcttact gctgccactg
     1201 ggattacata gacttcccag cctacctgcg gataaaagag aatagtcgca ggcgaaatca
     1261 tttgaatcaa aaattggtgt aaatagaaac cagctaccag accaagtacg attcccacaa
     1321 gggacagcac aatcgtctca cggtaaatgt agagggtgac ttcattatta tgaaaaccga
     1381 gaaccttgat agtggagagt tcacggattc tctcagctac gttgatattg gtcagattgt
     1441 aaaggatgac aatagctaat agaaccgata cgatgaccaa gatggtcatg gtctgattga
     1501 gtgagctagc gatagagtcg aagagtcgaa tggctgaagc attttggaca acactggaca
     1561 ccgcagattg attcataagc aagcccgcct gactttcgat actagttgca ctggtatccc
     1621 ttaatgagac cagataagtg ttggcttggg gtagctgtcc gtaaagttgc tcatagctag
     1681 cctgactcat ataaataaag tgaccaacgt agttctcagt aatagcagcg acctttagtt
     1741 ccttaccttc aatttctaaa gtctgcccaa ccttgacacc tgccagctgg gcgagtttag
     1801 ctgtaataac gatgccatct tttaatgtca gctcctgctg atgatgttga agatggataa
     1861 agggagtcaa atcttccttc tctatcatca taagagtaat gttttgaaga ccagccctgc
     1921 ccttgaaatc cttgtctagc gttttagaat agattttctg gtaggctagt atctcctgcc
     1981 ctttcaacac ttctgctagc tctaccttgt cctgattggt cgcactagga ttttcagaga
     2041 caagcatctg atactgttgg atttgttgaa actgtttaga cggaactcct gctacagaag
     2101 attggattcc caaacctgca aagagcagag ctacagaacc tgccacacca aagattgtca
     2161 tcaacattct ctgcttataa cgaaagatgt tgcgggctgt taccttatga gtaaaactga
     2221 gacgacgcca gataaaaccg atacgctcca ataagatttt agctcctttg acaggaggtt
     2281 taggaagtag aagctgggct gcttcgtcat gaagttccct ccaagccacc agataggctg
     2341 gtaacacact cgccaaccag ctcaagacaa gagctagtaa gctataggtc caatagaact
     2401 gaatctgggt ttctcccacc accatgcctt ttgtaatgac acttgaaatt acactggcta
     2461 gcaaataatg accaagtata ctacctagag ccgttccgac agtcccagct actagtccat
     2521 aaaggagaaa cttggcgata atatccttac tacgataacc caaggcctta aaaatccctg
     2581 catgagttcg ctcttcgtct acaaagcgag tcatggtcgt aaaggtcacc atggctgcta
     2641 cggcataaag taccacagga aagatattgc ccactgctcg aatactggat gaagcattgc
     2701 tatacataag atagccctga ccacctggca tggtctgacg attataaacc tgataccttg
     2761 gctccgccaa atcatccaag acttgctgat gtttttctaa tttttccttt tcttgggcta
     2821 gattttgttc agcttgcttt agtttgtcct cttccttgcc cagttcctgc ttggcttggg
     2881 taagttgagc actagcctgc tctctctgag cttgaggaaa caaggctagt tgactttctt
     2941 gagcctgtat acgagcttga gcagctgcta aacgacgctt gccttcctgc aaattagtct
     3001 gagccttgtc aagagtctct tgccccttgt ctagagactc ttgtccttct tttttcaaaa
     3061 gttgcagacg taccttgcca ttatctgata aagtttgttc aaggtcttcc tgatgttgct
     3121 tggatttttc ttcataagct gatgaaaagg catttaaacc tgctaaatct tgatatttca
     3181 aacgagctat attgtagact ttctgatcaa attgactagg taaaatcacc ccataggctg
     3241 tcagagtccc acttccactt cctgcgtagc ccatatctcg ctgggagagg atttcagccg
     3301 aatccacaaa accagtaatg gtataagtat ggtcttttaa agaggaatga ccctcttctt
     3361 tttctttaaa actaatctcc tgtcccacgc tgtattggcc ttgcaaatga gtggccaaag
     3421 cgatttccct gtctgactga ggaagtcgtc cctttcttag ctgaaaggtt gaaattcgct
     3481 ctggtttgga gtacagtcga atggcatcct gcccattatc catagtcaca tctgtcaaat
     3541 agccaaactc gacctctgcg ccctccgtct gttttagttc ttcttggtct gcttgatcca
     3601 agccatagtt agacatgact gccaaatcca aggtttgagc agttgttaaa taagcattag
     3661 ctgtcgcctc catgttggga ctggttactt tgaggcctac taaggctaga gatcccaaca
     3721 tcatcaggat caagatggat aaaaaacgcc ccttggagtc tgtgaaggac tgaattaagt
     3781 ccttccaata agtttttcgc ttgatcatgc tagtactcca aactgtcaat atcctgagga
     3841 tgctggttga gcaccacatc cttgacactg gcatcgtgca tatgaatcac gcgatcagca
     3901 atgggcgcca aagctccatt atgagtcacg atgatcaccg tcgctccctt ttgacgagac
     3961 atgtcttgga gaattttcaa aacctgcttg cccgtctgat aatccaaggc tccagtcggt
     4021 tcatcacaaa ggagaatttt aggatttttg gctaccgcgc gtgcaatgga gactcgctgt
     4081 tgctcccctc cagaaagctg ggctggaaag ttattgagac gatgagccag acctacatct
     4141 gtcaagacct gatcaggatt caaggcatct gtcacaattt cagaagccag ttccacattt
     4201 tccttagctg tcagattaga aactagatta taaaactgaa aaacaaaccc cacatcattt
     4261 ctacggtaat tggtgcgctg gtgggaacta taatccgcaa tattaacacc atcaatccag
     4321 atttcccctt catcattggt atccattccc ccaagaaggt taagaactgt tgacttgcct
     4381 gcacctgaag caccaaggat aataaccagc tccccctttt caatctcaaa attcacatca
     4441 cgattggcca caatctccgt gtccccaacc tgataacgct tgtaacagtg tttcatctca
     4501 atataagcca tcagacccac cctctatcaa acaaattata ctcctcgaaa atctctcttc
     4561 aaaccacgtc agcttccatc tacaacctca aaacactgtt ttgagcaacc tgcggctagc
     4621 ttcctagttt gtgctctttg attttcattg agtattactt cctattatca ttataacgct
     4681 ttttcaacta gttggaaact gcctatattc ttaaatcctt attaaaagga agtttcaaat
     4741 tactgcctta taacatcttc ttaaaaataa aattgaacaa gatcaccttc tgctgttgcc
     4801 accgaaatac aatctttccc catgcatttc gcttttattt tttcttgatt cattttaatc
     4861 acactcttct caatatctgg ataagaaact cgcaaatctc ctccactcct tgataaaatg
     4921 gtcaactgta agagtttttt atcttcccag ctcatgctca cttcaaaatg tccacgtgcc
     4981 attaagcctg aaacagaacc tgttgaccaa gcatcaggta gggcagctag aggtaccaga
     5041 taagctgcat gagactggag taacatttct gccatgccac tagtagcacc aaaattacca
     5101 tctatctgaa aaggaggatg gctacaccaa agatttggca aggtggatgt ttttaactgc
     5161 tctgccaata atttatgggc tcgattgcca tctcccaaac gcgcccagag attgatctta
     5221 ttagccttgg accagcctgt gccgccatct ccacgatcat tgaggctagc acgtgccgct
     5281 tcaatatact cttgtccctt gtagctaaag agattgccag gatagagtcc cactagatgg
     5341 gaagcgtgcc gatgctgggc ctccactttc tcattttgaa aatactgctc ttcctcctca
     5401 taccactccc tgattcgacc agattgagtg atttgcaaag gatttagtaa atcagacttc
     5461 tccttaacct cagtcaacaa gtcctcatcc agtcccaatt cctgagcagc ctgaataaaa
     5521 tcatgaaata actgccaaat cagagattgg tcataagtat tgccaatcga aatcggccca
     5581 tgttctgggg aataagacgg agaagacacc caacgctgcg cctgctgatc cttatgtaaa
     5641 aaggcattcc aaaaacgaac cgtttccctc aacatgggat aaattttctc cctgagatag
     5701 tcttggtccc tataaaatga ataggcttca taaacagttt gcatcatcca cgcattggca
     5761 gctggtgacc aaccccaata gtaatcccaa ccaggtgccg tccaaccaaa gggagtcgct
     5821 tgagtatgaa ccaaccaacc attttcctca cctttttgag agacgattcc tgcatacttt
     5881 acagccgcta gacgaccata gacacgcaaa tcatctacat agttgatgac cggaaagacc
     5941 gcctctagga gattggtaac ataggctggc caataattca tctgcagatt gacatttaag
     6001 tgatagtccg aattccaagg aggattgtcg accgcattcc agactccctg taggttagct
     6061 ggtagagcat ctgggcagtc tctggacgaa ctaatcaata aataccgtcc atactggaag
     6121 aacagctcct ccaaagcctg cccttcttgt ggcttataat tttttaacaa atcatctgta
     6181 gtgaatgcgt caacattagc ttccaaatcc aattgaacac gctggaataa ggcttggtag
     6241 tcctcgatat gccttgattt caattgggta tagccctttt ctttagctgt gtccaccaag
     6301 tctatcacct gttgctctaa atctagtttc ttgcgataat tgctagcagg attttgggca
     6361 aaatccgtct tagcggccaa gaagagattg gcataactgg ctcctgatat ctgaacccta
     6421 tctgaccaaa ctctaatatc tccatccgtt tcccaagcta gataactagc aaaccgcaga
     6481 tcattatcct taactcttcc cttcatcaag atatgagaat cagtaatatc caacttacac
     6541 tccttgtaat cagatttttc ctgctcatac tttccatcag aagccaaatc acaggtcaag
     6601 gatagttcta tagtaaaatc tagagtttcc gccccttcct tagtaaaacg ctgaaccaag
     6661 agatcatctg gaaaactcgc aaaagcttca cgttcaaatc gcgttccctt atagacataa
     6721 gaagtcgtca caagtgcctt actaatattc agctgtctct gatagtccgt cacctgagac
     6781 aaagtcgtac cttgctggct gaactcaatg tgaatatccc caaaagacag ataggtccca
     6841 tattgactcg tttttggccc aattaggtgc tgctcagcca gttccttagc cagattgtaa
     6901 tctctcttct ccaaagcctg ccgaatctca gctaaaaaaa catactgatc ctgaagattt
     6961 ccaccctgat aatctgaact atcaggaagt ggacctccag accagagact tttttcatta
     7021 aattgaatcc gttcagcccc tataaggcca aatacttttg ctcctaaaga accattgcct
     7081 atcggtaaag cttcttcttc ccaacccatg taagtggttg aaactggttg cttgtaggcc
     7141 agtacataat cttgtttttt attccttatc atacttccat actaacataa aaagcctaga
     7201 aatcaattct agacttacaa ttttttattt tccaaggtag tattcagaaa cgacgttcaa
     7261 tttttcgtcg aattcgaata ccaatggtgg gaagttaggg atttccacgt ccatgatctc
     7321 gtcatctgac aaacctttga tgtgttttac aagggcacgg attgagttac cgtgagctcc
     7381 tacgaatacg tttttaccat ctttaagagc tggagcgatt ttatcttccc agaatggaag
     7441 agcacgttcc aaagtcactt tcaagttttc agcatctggg attactgagt cgtcaagtga
     7501 agcataacga cggtctgtgt gagctgagtg ctcatcatca cggtccatat ttggaggcaa
     7561 tacatcgtat gaacgacgcc agatgtgaac ttgctcatca ccaaattgtt cagcagcttc
     7621 agctttattt ttaccagtca aaccaccgta gtgacgttcg ttcaagcgcc atgatttttc
     7681 aactggaacc cacaattggt cagaagcttc aagagccaag ttagttgttt tgatagcacg
     7741 tttcaatact gaagtgtaag cttggtcaaa tttgatacca gcttctttga tcaatttacc
     7801 agcgtcaatc gcttgttgtg tacctttttc agacaaatca acatcagccc aaccagtgaa
     7861 aaggttagct ttgttccatt cagactcacc gtggcgagca aaaaccaatt ttaccattag
     7921 atggattctc ctttaaattt tccgaggttt cccctcgttt atctattcta ttttacacaa
     7981 tttttcacaa aaaagctagt caaaaccaaa aaggaaagga ctgtttgaca atgtttggca
     8041 atcacattgt ttttagttgt ttctattcta tcttcattat ttaacctttt tttctgcgca
     8101 tcgaataatt tttgagttga ctcttttagt ttagtaaacg agtcattatt ttccatagct
     8161 gtgcctttga aaccttcctc agctttagta aaataatcat caaaaata
//
LOCUS       DAVMEI010000082         8050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVMEI010000082 DAVMEI010000000
VERSION     DAVMEI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.082"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(88..1428)
                     /locus_tag="VYQ24_001356"
     CDS             complement(88..1428)
                     /locus_tag="VYQ24_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1968490.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(1442..2179)
                     /locus_tag="VYQ24_001357"
     CDS             complement(1442..2179)
                     /locus_tag="VYQ24_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1968491.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(2184..2522)
                     /locus_tag="VYQ24_001358"
     CDS             complement(2184..2522)
                     /locus_tag="VYQ24_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1968492.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            2798..3127
                     /locus_tag="VYQ24_001359"
     CDS             2798..3127
                     /locus_tag="VYQ24_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1968493.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(3295..4344)
                     /locus_tag="VYQ24_001360"
     CDS             complement(3295..4344)
                     /locus_tag="VYQ24_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968494.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(4341..5072)
                     /locus_tag="VYQ24_001361"
     CDS             complement(4341..5072)
                     /locus_tag="VYQ24_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968495.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            5140..5550
                     /locus_tag="VYQ24_001362"
     CDS             5140..5550
                     /locus_tag="VYQ24_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1968496.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            5560..5847
                     /locus_tag="VYQ24_001363"
     CDS             5560..5847
                     /locus_tag="VYQ24_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968497.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            6009..6203
                     /locus_tag="VYQ24_001364"
     CDS             6009..6203
                     /locus_tag="VYQ24_001364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968498.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSNHTHD
                     KPTLTWFEEIFEEYHSPFHN"
     gene            complement(6537..7673)
                     /gene="dnaJ"
                     /locus_tag="VYQ24_001365"
     CDS             complement(6537..7673)
                     /gene="dnaJ"
                     /locus_tag="VYQ24_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1968499.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         2559 a         1806 c         1503 g         2182 t
ORIGIN      
        1 ctcattacat cacataagaa cttcatcatc caatacaact ataaaaaaat aagaccgaga
       61 aacaagtact ctcggtctta tttttcatca ttctgtatgt atcacagtaa gtacctgacg
      121 aaagacttga ttttgacagg tggtatttag actggtatta ggatggcttt ccacaatctt
      181 catgacggta tagagaccaa ctcctctctc ctccccttta gaactggctc caaaggagaa
      241 gatttcagaa atatcgatgc cctcttcttt gatggagttt tcgatgataa aggtctcctg
      301 tgctccattt tttaaaaagg cgattgaaac atgaggttga ctagcttcca cactggcttc
      361 aatagcattg tcacaaagga tagacacaat ggttagaaaa tcaagtagac tcatcccctc
      421 gacctgaatc tcctcaggaa cttcgacatt aaagacaatg ttcttatctc tggcttttaa
      481 aaatttccct gctagaagac ttttgagggc tttatcacga atatttacca atctgcccag
      541 gtcatattta ttgttctgca atttctgact ggaatccttt aagacggagc catagacctc
      601 ttttatctgc tccatatcct cctcttcaat gcccagacgt aagctagtca agaggttggt
      661 ataatcatga cgaaagctcc gtacttcctt gtaaagctcc tctatatgcc gactatagcg
      721 ttccatatct ctatagcgca gggcctgctc ttgttccaat ctctcataga gtttttcctt
      781 caaataggta tccaatttct tgataacccc cataaaaaag agtaggtaaa agactaggat
      841 gagatggcga acagtctttg attgaatact ttgttcatat tcaaaaaaag acagactttc
      901 catgactaga tagtagccac ccattatcca gttaatctga gtcagggact tttgaaaggc
      961 tttatcgaga atctcctttc tcaagctagt aaaatcgtag tccaaccatt tcaaaaaagc
     1021 tagagaaatg aagaaattga aaattattat acataaccca gtaaatgagt agccatcata
     1081 tacttgccct tgtcccaaaa atggaagcac aaaataggag actcctctat aaaagagatt
     1141 caccaatatc attggaaaga gaccataaaa gaaaaggagt tttttaggaa gccctctcaa
     1201 taataagaaa gataagccta tgccgtacaa gggttccata aaataagata ggtaaacatt
     1261 tcctactata tagctaatca tcacaaaaac aaaggccaac agtatcttaa aaagaaaggc
     1321 cttaaaaatc ctctcgaaag taagatcaat tccatccacc ttaaagaaga tgacaatttc
     1381 tagtccatta gtaacaagtg tatacaacaa tatccaagca atgttcataa attctcctag
     1441 ctcagtgtaa tttattgatg gcctcagaca cttccctgac cttataacgg gcgattagac
     1501 aacttccacc attgggaaag aaaagcagtt tttctttctt atccaaatgc accacatttg
     1561 caggattgat gagaaaagag cggtggcact gcaagagacg gggctcctgc ttgaaaacct
     1621 cctctaaact cgctgtaaat tccagcctgt ctgtcttggt atagagaata acacgatggg
     1681 ctctgggcga cgtttcgaga tagtaaacct ctttaaaagg atactgaaat tgggcaaatt
     1741 ttgatttaaa gtaaaagcaa tcttccgcca gacttttact atcttgacta ttggcataga
     1801 ggagggctgt ctcgatccga gattcaaact cctctgctga caaggcctta tcaatgtagt
     1861 ccaaagcaga cacttggtag cgaaaagaca ggggcataaa ctccgagtga gtcgtcacaa
     1921 agacaatcag ggcataagga tcccgatccc gaatctttct agccacttcc agtcccttca
     1981 tctcttcatt tcgaatctca atatccaaaa agaatagctg atgggccccc ttctcatgca
     2041 cttcagccag cagttggtcc ggcttgccaa atacctcaaa agagctagga atgatatgat
     2101 gtgctttcaa aagtttctca atcgtcgttt caattctagt ctgttgggaa aaatcatcct
     2161 ctaaaacaaa tattctcatc ttcttactct ccctctttca accatttttg acgaatttct
     2221 ctatagcgac gtctggcata cttgacagcg tattgacctt cttctccgag aaaaaggatt
     2281 ctttcttgaa aatcaatcga ttttaattta tcaagattaa ccaaacaatt tcgatgacat
     2341 ctcatcaagg tttccccaca ttggttctca agattactta aattttgaag catattaaaa
     2401 ctagcttctt ctgtaacaat ctgtacggta tgggctttag ttggatgtgt ttggatatag
     2461 tagatatcgt cgatgtttac tttatagact gttttctgcg tctgaataat catatatttc
     2521 attggattct cctcctaaga aaagaataac agaaaaggtc tagcccaagc acacccaatc
     2581 ctaaacagtc tttgaaaact cctaaaagga ttattttcta caacatcatg atgagcttac
     2641 aactatttta tcataaaatt cactcagcgc aattcaagac atttcaatga caattaaaga
     2701 tttgaataaa aaaaaagaat taaaagtgat aaactaacag tatcaaaaca cagttgccag
     2761 aagtaagact ggcacccaga ttaaaggagg cattcccatg acagatacag accccatcaa
     2821 aagagctcat actttgatta ctgacttaaa caaagcctac caagcatgca aacaggcaag
     2881 cgctgacgac gtccgctttc aggagcaatt aaactctatt cttggttttc tagccaaggc
     2941 tgaaacagtg gataatcgat tcttgattga actagaaaaa ttttaccaga cttccagtct
     3001 tctcatggga ctcagcgctc ttgatccaga tgctccaact cgcgccgctt ggcgggccta
     3061 tgaccgcttc cactttgacc aagtcaagac caagttaata ctcaatgaaa atcaaagagc
     3121 aaactaggaa gctagccgca agctgtactt gagtacggta agacgacgct gacgtggttt
     3181 gaagagattt tcgaagagta taagtctcta cggaccaacc attatcctgt agtaacaaaa
     3241 aagaagttga gacagactga actcaacttc ttttttagtg tcgtatcagc aatgttagtc
     3301 ctgcatctga cgttttacct gataaggcaa atacaagact agtaaaacca aaccagctcc
     3361 tagtaaggct agaagggcta gtttttcttg gaaggtaatc aacccaacaa ctaattccac
     3421 aagtaaaaca aaactggtca atcctcgaac tacctcctgt aagccttttt ccttttgccc
     3481 cttgtccagc ggaaagagtt gggtcaaata ctggtagtca aaggcatgat agagggccag
     3541 caactggaag agcaagaggt agttaaagag aactaccact gctgtcgcaa tccaagcttg
     3601 ctcgataaaa acctgcgcca gtacggaaag taagagaaga cgaagactga gagcaaagag
     3661 gtcgccattt cgcagataag aacgcagata gagattttgc caaatcttcc caggcacctt
     3721 ctgaacagcc tttaagataa agtccagata ggcacgacgc ttaacgctgt ttgaaattcc
     3781 cttgacctgc gtaaagaggg caaagaaacg aagcaagact tgcttacgct tgctttcttg
     3841 ggaaatgaca tagtcccaat ccagtccagt ttcagtgaaa aatttgctgg ccttttgacg
     3901 aaagtggaaa tattttccta cccccaataa aagcacatag agcagaaaaa ctggcaagcc
     3961 ataacccatt gctaaaaata aaggcgcaaa taacagcaag aaaagggtct gtacaaagag
     4021 ccaaaagact agggaaatgc cagtttgacg cttgagatgg agcttgattt cctcttctcc
     4081 aactaagaga aagagcttgt ctggagcctc catataggtg gcaattcctc cccaaagtaa
     4141 aagtaaaaca gacgtaattc ctacaaacaa aaggataggc caatgatttt caggaaaatg
     4201 ttgtaagagt tgactgtact ggtaggctag aaagcccaac aggacaagca ggaacaagac
     4261 aaagtggtca ttgagcacat agcgcagata accaagacac tccttacgaa aggcctgctt
     4321 tctctttaaa aacaagtctt tcataggtcc tcctcttttg tcagagccaa gtaaatatca
     4381 ttcaaactag cctcaggcat atcaaaggct tcgcgtagtt gcaggagatt gcctttggca
     4441 cgcacctctc ccttgtgaag aatgacaaag gcatcacaca tcttctccgc cgaatccagc
     4501 acgtgggtac tcatgagaat agacttgccc ttttgcttct ccacttccaa aagctgaatc
     4561 aaatcagaaa tagccagcgg atcaagacca aggaaaggct catccacgat gaaaagactt
     4621 ggatccgcca caaaagcaca gataatcatg accttctgct tcatcccttt tgaaaaatga
     4681 acagggaacc agtctaattt ctgttccaaa cggaacattt ttaacaaggg ctctactcgt
     4741 tcgaaagcca ctttttgctc aataccgtaa gccatagcaa ccgtttcgat atgctctctg
     4801 agggtcaatt cctcatacag actaggcgtc tcaggaatgt agccaatctg cttgcggtag
     4861 ctagtcgcat ctccttgcag agtcaggcca ttgatattga tggagccact ataaggtgcc
     4921 aacagaccga taatctcatt gatcgtcgtt gatttcccag caccattgag accaatcaaa
     4981 ccgaccaact gcccactttc aacagtaaag gacacatctt tcaaaacagg aacatgaaca
     5041 tagccacctg tcaggttttt aatttctaac atattttctc caaatctggt ataatgtagc
     5101 tatattatat caaaattcag tacagtagag gtagatttta tgtcagattg cattttttgt
     5161 aaaatcatcg caggggaaat tcctgcttca aaagtatatg aagatgagca ggtccttgcc
     5221 tttcttgata tctctcaagt aacactagga cacaccttgg tcgtgccaaa agaacactat
     5281 cgcaatcttt tggagatgga tgctacgagc gccagccaac tctttgccca agtaccaaaa
     5341 gtagctcaaa aagtcatgaa agtcactaag gctgctggta tgaatatcat ttccaactgt
     5401 gaagaagtcg ctggtcaaac agtttttcat actcacgttc accttgtacc tcgctacagt
     5461 gctgacgatg acctcaagat tgattttatc gcccacgaac cagactttga caaacttgct
     5521 caagtcgctg aaaccatcaa aaacgcttaa ggagttgcta tgaaattatc caacctactg
     5581 ctatttgcag gagctgcagc cggaagttat ctggtcacaa aaaatcgcca aaccatcaca
     5641 gatgaagtct tgaataccac tgaccgcgtt caagctatca aggacgatgt ggatattatc
     5701 caaaacagcc tgcaaatcat tgaccagcaa aaagaactta tcaaggaata ccaagaagac
     5761 ttgacttaca agtttaaggt cttggaaaag gatatccaga ctagactagc tgtgataaaa
     5821 gaaatgcagg gaactgaaga taagtaaaaa gagcccgacg gctctttttt aaaacgtaaa
     5881 caagttatag tgaattgaat ttagaatagc acatcatagt ttctaaagca ttgttagaga
     5941 ttactttaaa atcctcttat caatttgttc atattctatt tcaatctact atatatatcc
     6001 catcaactat gcatcataat ttaggtaact catactcaat aaaaatcaaa aagcaaacta
     6061 gaaagctagg cacagactgc tcaaaacacc gttttaaggt tgtggataga actgacgaag
     6121 tcagtaacca tacccatgac aagccgacgc tgacgtggtt tgaagaaatt tttgaagagt
     6181 atcattcacc atttcacaac taaatcgaag actttctgcc gtaatgaaaa cacctgaaac
     6241 agcttggttt cggctatccg gaaactttaa gacttaggtt caaagtttag gtatggaact
     6301 tcgaaggagg tcgctaccgt ccgtcattac ttaggaaagt cttaaaaaat ctataaagta
     6361 aaaagagccc gacagctctt tttgtttata ctcaatgaaa atcaaaaagc aaactaggaa
     6421 actagccacg ggctgctcaa aacactgttt tgaggttgta gatacgactg acgaagtcag
     6481 tcacatatat acggcaaggc aacgctgacg tggtttgaag agattttcga agagtattat
     6541 tctccatcaa aggcatcttt aatatggtca aagaagcctt ttttctttgg atttactttc
     6601 aagtcaccag cagccgcgaa ttctttcaag gctacttttt ggcggtcgtt caagcctgtc
     6661 ggtgttacga cattaacagt aacgtattgg tcaccaactg caccgccacg aaggctcggt
     6721 gcccccttac tacgtaggcg gaacttctta ccagtctgag ttccctctgg aataaccaat
     6781 tcaacatcac cgtgaacagt tggaatatct actgtatcac caagagccgc ttggacaaag
     6841 ttgaggttga gattgtagaa gatagtcgtt ccttcacgtt caaacttgtc gctagcttcc
     6901 acagaaacta ctacatacaa gtcaccataa ggtccaccgt taaagcctgc ttcaccttga
     6961 ccagcgaggc gaatttgttg acctgtttcc acaccagcag ggattttcac atgtacgcta
     7021 tgagcttgtt tctcatgacc tgttccatga caggttgtac atggatattt gatttctttt
     7081 cctcgaccgt gacagacatc acaggttact tggcgacgca tcataccaag aggagtctgc
     7141 gtatcgacgt taatgacacc agcgccatga cagcgtccac aagtgactgg acttgtccct
     7201 ggcttagcac cagatccatt acatgtacga cagccagctt cacgatgata cttaacttcc
     7261 ttctcagttc cgaagatagc ttcttcaaag gtcaaattga cacgatactg gagatcatct
     7321 ccttggcgag gagcgtttgg attgcgcgaa gaaccgcctc cgccgaagaa acttgagaaa
     7381 atatcctcaa aaccaccgaa gccacctgcc ccattgaaac cgccgaaacc accagctcca
     7441 ccaaaaccac cattggcgcc tgcagcacca tactggtcat aggcagcacg tttttggtcg
     7501 tcactcaaag tctcataggc ttcttgaact tccttgtact tgtcctcagc accaggctcc
     7561 ttgttgatat ctgggtgata ttttttggaa agcttacgat aagccttttt gatttcgtct
     7621 gccgaagcgt ttttggatac ccccagacga tcataaaatt cagtattgtt cataattcaa
     7681 gatacaaagg gcgtcaaacg gacacagcca tctaggagtt ttgacgacgg acgcttacgt
     7741 cctagaaaaa actatctttt tggcacagtc catagcccgt gttcagttac gaacaccctt
     7801 attcctttct attgatgttc tgaatcgaac caccatttag gtcgggttca attcctttct
     7861 tttatattta gtaagaaatt ctgaaacccg tgttcagtta cgaacactct ttgttccttt
     7921 ctattgatga gacaaaaccc aaacaaatga gattccgtcc ctttttatcg aaaaacagag
     7981 gctgggttgc aacctctgca aataagtaaa ttaactttat tttgatttta aatatcctat
     8041 aagtacagaa
//
LOCUS       DAVMEI010000083         7864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVMEI010000083 DAVMEI010000000
VERSION     DAVMEI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.083"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            197..433
                     /locus_tag="VYQ24_001366"
     CDS             197..433
                     /locus_tag="VYQ24_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1968500.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCHRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            664..1950
                     /locus_tag="VYQ24_001367"
     CDS             664..1950
                     /locus_tag="VYQ24_001367"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1968501.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            2151..2618
                     /locus_tag="VYQ24_001368"
     CDS             2151..2618
                     /locus_tag="VYQ24_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1968502.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            2651..2744
                     /gene="ffs"
                     /locus_tag="VYQ24_001369"
     ncRNA           2651..2744
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYQ24_001369"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2827..3968)
                     /locus_tag="VYQ24_001370"
                     /pseudo
     CDS             complement(2827..3968)
                     /locus_tag="VYQ24_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4023..5093)
                     /locus_tag="VYQ24_001371"
     CDS             complement(4023..5093)
                     /locus_tag="VYQ24_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HEV1968503.1"
                     /translation="MEIDKVKADLKQVGKRVADLSQSITNEEQTKNAFIMPFFQALGY
                     DIFNPLEFVPEFTADVGIKKGEKVDYAIILDGEPQILIECKSITENLTKHDSQLFRYF
                     VTTKSKFGILTNGREYKFFTDLDEPNKMDTTPFLTIDVTDIKENQFTEIIKFHKENFD
                     IDNIVSSASELKYLNNLKAFLTENITTPSDSFLRYLTSEIYEGRVTQNILTTFSPIIV
                     KGFNQFITERVNEKLSAALNTSVETKVTTDIPKVEAEAEEIVEVTDEIITTPAELEVY
                     TVVKMLARDVVSPERVFYRDNRSYFNVLVDDNIKKWVLRYRSNSKKSTIEIRDKGIFP
                     VSTPLEVANYANEILEVIKKFS"
     gene            complement(5110..5490)
                     /locus_tag="VYQ24_001372"
     CDS             complement(5110..5490)
                     /locus_tag="VYQ24_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1968504.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHNSGFYNPDTNVLAIDHNLTPE
                     QQIQVALHELGHKDHTRSEHQNARLRCENEADRNMIHHLVKDALENLDDPTEFDYLKF
                     MSYYNLKTMTNEIMVKEEYLALVN"
     gene            complement(5503..5766)
                     /locus_tag="VYQ24_001373"
     CDS             complement(5503..5766)
                     /locus_tag="VYQ24_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1968505.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(5766..5999)
                     /locus_tag="VYQ24_001374"
     CDS             complement(5766..5999)
                     /locus_tag="VYQ24_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968506.1"
                     /translation="MTITINFTEKNSYITDYLNKHGIDTTTMDFDDFMALMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLTREDIA"
     gene            complement(5999..6367)
                     /locus_tag="VYQ24_001375"
     CDS             complement(5999..6367)
                     /locus_tag="VYQ24_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968507.1"
                     /translation="MFETFEKIKELAKKRGKALGQVEEDLGYGRNTLYKIKNSTPNAE
                     RIAEIANYFNVSTDYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            6940..7131
                     /locus_tag="VYQ24_001376"
     CDS             6940..7131
                     /locus_tag="VYQ24_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1968508.1"
                     /translation="MPDIANGRERVIAFLKEKGIKKATLAVAYGFKRQEVTNILSGTT
                     KGPRANSFILQVIEDYGIE"
     gene            7154..7357
                     /locus_tag="VYQ24_001377"
     CDS             7154..7357
                     /locus_tag="VYQ24_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968509.1"
                     /translation="MRPKRYPYSGQKESTFVKADPELVEKLLRNTSFLECLQKKPINF
                     QIDSEEFKRLSYEAIHDTSQVTQ"
     gene            complement(7512..7679)
                     /locus_tag="VYQ24_001378"
     CDS             complement(7512..7679)
                     /locus_tag="VYQ24_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HEV1968510.1"
                     /translation="MANTPIKPGTDNQKPGRYVEVGPRGGKVTNGHTATIGKGDRLPP
                     TSAKGNGWKKV"
     gene            7699..7864
                     /locus_tag="VYQ24_001379"
                     /pseudo
     CDS             7699..7864
                     /locus_tag="VYQ24_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
BASE COUNT         2222 a         1227 c         1660 g         2755 t
ORIGIN      
        1 ttacccaaaa gttttaaggc ggtcttttta gaactttaat tgtttgaaat ttaggtagca
       61 aatttgtttc tattttgtca acttttccta tttttatctt gttgaggctg gtattttaac
      121 aattcaggaa ttgatagtga atgtgtaaaa ttttttgtta gaataagttt ataaaaaaga
      181 aaaggagtat ttgattatgt tacaaaaaat ttatgagcag atggctaatt tctatgatag
      241 tattgaagaa gagtatggtc ctacatttgg tgataatttt gactgggaac atgttcattt
      301 taaattttta atttattatt tagtgagata tggcattggt tgtcataggg attttatcgt
      361 ttaccattat cgtgttgctt atcgtttgta tcttgaaaaa ttggtaatga atcggggttt
      421 tatttcttgt tgaggtaatt ttagtaaatt tccgaactaa tttactcttt tatgtaaaga
      481 taatagtaaa tagctagtaa tttttctaaa tcatttttta atagttggaa atagcaaatc
      541 tttctattgt ttcttcttga taaaaaggcg attttttatt ataataaatt gtaagatata
      601 attgcaggtg agagtcctgc catgtatgtg agaaaggaag agcctgaggg ctcagacaaa
      661 attatgactt cagttgttgt tgtaggtacc caatggggtg atgaaggtaa agggaagatt
      721 acagacttcc tttcagcgaa tgcagaagtg attgcacgtt accaaggtgg tgataatgct
      781 ggtcacacga ttgtgattga cggtaagaaa tttaagttgc acttgattcc atctgggatt
      841 ttcttccctg aaaaaatatc tgtcattggg aatggtatgg ttgtaaatcc taaatctctt
      901 gtaaaagagt tgagctatct tcatgaggaa ggtgtaacaa ctgataactt gcgtatttct
      961 gatcgtgcgc atgttatttt gccttatcat atcgagttgg atcgcttgca agaagaagct
     1021 aagggcgaca ataagattgg tacgacaatt aagggaattg gtccagctta tatggacaag
     1081 gctgctcgtg ttggaattcg tattgcagat cttttagata aagatatttt ccgtgagcgt
     1141 ttagaacgta accttgctga aaagaatcgt ctttttgaaa aattgtatga cagtaaagcg
     1201 attgttttcg atgatatttt tgaagaatat tacgaatatg gtcaacaaat caagaaatac
     1261 gtgatagata catctgttat cttgaatgat gcgcttgata atggcaaacg tgtgcttttt
     1321 gaaggtgcac aaggtgttat gctagatatc gaccaaggta cttatccatt tgttacgtca
     1381 tcaaaccctg tagctggtgg tgtgacaatt ggttctggtg tcggtccaag caagattgac
     1441 aaggttgtag gtgtatgtaa agcttatacg agtcgtgtag gagatggtcc tttcccaact
     1501 gagttgtttg atgaagtggg agaacgtatc cgtgaagtgg gtcatgagta tggtacaaca
     1561 actggtcgtc cacgtcgtgt aggttggttt gactcagttg tgatgcgtca tagccgtcgt
     1621 gtttctggta ttactaacct ttctttgaac tctattgatg ttttgagcgg tttggatact
     1681 gtgaaaatct gtgtggccta tgatcttgac ggtcaacgta ttgactacta tccagctagt
     1741 cttgagcaat tgaaacgttg caagcctatc tatgaagagt tgccaggttg gtcagaagat
     1801 attaccggag ttcgcaattt ggaagatctt cctgagaatg cgcgtaacta tgttcgtcgt
     1861 gtgagtgaat tggttggcgt tcgtatttct actttctcag taggtcctgg tcgtgaacaa
     1921 acaaatattt tagaaagtgt ttggtcctaa gagattttta agatttgttt aagataggtc
     1981 gggtatacta tagacagtta caagaagacc tcctaacttg ttgtaacaaa tatcctaaac
     2041 ttttcttttt cataataatc tccctataga gtcaccgcat tcggtggctt cttttgtgtt
     2101 gggattcatg atataataat aaaatcgata agtaggaaaa gagaaaagag atgtattata
     2161 cgcttgaaga aaaagaagtc tttatgaggg aggctttgag agaggctgag attgctcttg
     2221 aacacgatga aattccaatt ggttgtgtga ttgtcaaaga tggggaaatc attggtcgtg
     2281 ggcataatgc gcgtgaggaa ttacagcgag cggttatgca tgcggaaatt atggctatag
     2341 aggatgcgaa cttgagtgag gagagctggc gcttgctgga ttgcacactt tttgtgacca
     2401 ttgaaccttg tgtcatgtgt agtggggcga ttgggcttgc ccgcattcca aatgtggtct
     2461 atggggctaa aaaccagaaa tttggcgctg ctgggagttt gtacgatatc ttgacagatg
     2521 agcgtctcaa tcatcgtgtg gaggttgaaa taggaatttt ggaagatgaa tgcgcagcta
     2581 tcatgcagga tttttttaga aatagacgga aaaaataatt ttgcttttaa aatgaatagg
     2641 aatgtgatat aataaatagt ggagcaacag ttctgcgtga agcgggtcag gggaggaatc
     2701 cagcagccct aagcgatttg aattgtgtgc tcttttttcg tgcactttaa aaacccttta
     2761 aaatcaacac tttaaggggt ttttgtttgt cttgtataag aaaaaggggc agacgagggg
     2821 cacaatttaa aattttatct tgtctaactt gctagatatg tctgatacca ttttttgagt
     2881 aacgtgagaa taaatctcta gtgtggtctt tgagtcacta tgccctactc tgtccatgat
     2941 ggcagtcaag ggaatgccca gctcagcaag tagggatatg tgagaatgtc taaatgtatg
     3001 tgtagttatg tttttttcta tgccgatttt ttgaccatgt cttttcaatg cacaaataac
     3061 cctggcattt gttattggtt ctcctagagt attgatgaaa ataaaatctg tatcaaatcc
     3121 atttgtcgca ttctctatta tttgctcttt gataatatct aacacttttt gaggtgctgt
     3181 tataacccta tcggacttga ttgtctttgg tgtagttctc tctttttgtc tgaaatcgta
     3241 tgtatgcttg atgtgaatag tctttttaga aaaatctata tcttccttgt agtttaaggc
     3301 tgccagctct ccatacctca tgccagtaag aaaaagaact ttagctattc ggatatactt
     3361 tgtaattcga taatcacata gggcctcgtc ttttaaattt tggatgaata acttaaactc
     3421 tttttggtct aagtattttg tatttttctt tctgagttcg tcggatgtaa ttacttttct
     3481 aggcgtttca acaaatagca tttcatttgt atcaatataa ttcattctga tagcaaattt
     3541 cattatctga ttgagcttga atttgatttt agaaacatag ttatgagatc tcccatcttg
     3601 taatagttga tctattactt tttgtaataa acgtctatca aatttctaac taggtagtcg
     3661 ccctctatct gctttaaaat ctcttttttt acattctttg aagcgtagac ggttgagttt
     3721 tttacgccgt gtttccaatt ctcctcaaat tcttcatata gtttttcaaa agttatatta
     3781 gaaacagaat gttgtttttc tcctaacttt tgttttatct tttcctgaag taaaatcgca
     3841 gcttgatttc ttgcttgggg agtttttttc tccatcgtta ctgagacttt ttttaatttc
     3901 tcagtatatg gatctttgta tctctcaaaa aatttatatt tgccgtttgg caattcttcc
     3961 atccacattg attttacctc acttttttga taaaatgggt atagtaaaga gggctttttt
     4021 tattatgaga atttcttgat tacttctaaa atttcattgg cataatttgc gacttcgaga
     4081 ggggtagaca ccgggaagat gcctttatca cggatttcga tagtgctttt ttttgaattt
     4141 gaacgataac gtaataccca ttttttgatg ttatcgtcaa ccaaaacatt gaaatagcta
     4201 cggttatctc tgtaaaatac acgttctgga gaaactacat ccctagcaag cattttaaca
     4261 acagtataaa cttctaattc agcaggagtt gtgataattt cgtcagttac ttcgacaatc
     4321 tcttcagctt cagcttcaac ttttggaata tcggttgtta ctttggtttc aacgcttgta
     4381 ttaagtgcag cacttaattt ttcattaact cgctctgtga tgaattgatt aaaccctttc
     4441 acaataatgg gagaaaatgt cgttaagata ttttgagtaa cacgcccttc atagatttca
     4501 gaagttagat atctgaggaa gctatctgac ggtgtagtga tattttcagt aaggaacgct
     4561 ttaagattgt tgaggtattt caattcagaa gctgatgaaa caatattatc aatatcaaaa
     4621 ttctctttgt ggaatttgat gatttcagta aattgatttt ctttgatatc agtcacatcg
     4681 attgttaaga atggagttgt gtccatttta tttggctcat ctaaatcagt aaagaattta
     4741 tattctctac catttgtcaa gataccgaat tttgatttag tagttacgaa atatctgaat
     4801 agttgagagt cgtgcttagt aaggttttct gtaattgatt tacattcaat taggatttga
     4861 ggctcgccat ccaaaatgat cgcatagtcg actttttcgc cttttttaat gcccacgtcg
     4921 gcagtaaatt ccgggacaaa ttcaagtgga ttgaagatat catatccaag cgcttggaag
     4981 aacggcataa tgaaggcatt ctttgtttgt tcttcattcg taatgctttg gctgaggtct
     5041 gctacacgtt tgccgacttg ttttaaatcg gcttttactt tatcaatttc catattgata
     5101 ctcctttttt taatttacta atgctaaata ctcttcttta accatgattt catttgtcat
     5161 ggtttttaga ttgtagtaag acatgaattt gaggtaatca aactctgtgg ggtcgtctaa
     5221 gttttctaga gcgtctttta cgagatgatg gatcatattc ctatcagctt cgttttcaca
     5281 gcgtagacgg gcgttctggt gctctgagcg tgtgtggtct ttgtgtccaa gttcgtgtag
     5341 ggcgacttgg atttgttgtt caggagtcaa gttgtggtca atagcgagca cgtttgtgtc
     5401 tggattgtag aaaccgctat tgtgccagtt tgaaccgtcg aagagacaaa gttctacttg
     5461 atattcttcg cagagtttag cgagtgtcat agttctcctt cgttatttat agacatctct
     5521 gcggtgggcg atttccacgg ctaggacgac tagtttatcg tcttggatat cacagatgat
     5581 gcggtaattc tctactctgt atcgccagta acctgcaagg ttggctttta gtgcttttcc
     5641 gtgttgtcgt ggattggtcg tgttttcaat gtttttagca agccaggata ggatttgttt
     5701 tctggttggg gtatctagtt ttttaagttg cttgagagct tttttatcaa tatctagccg
     5761 atacattaag caatatcctc tcgagttagt cctagttcat ccaagacctc atccatggta
     5821 taagtagctg ggtcggctaa atactccata taggcttggt cggctgctcg tgcgtcttcg
     5881 atatcttcca tgagtgccat gaagtcgtca aaatccatgg tcgttgtgtc gataccgtgt
     5941 ttgtttaggt agtccgtaat gtaggaattt ttttctgtga agttgatagt gatagtcatt
     6001 agcgttctcc tttgcttttg aagtgggcgg ataggacgga tgtaatgaag tcgatatcat
     6061 cttcatttag tggtttcccg tcaaataaca tggtgttggc tgctgctttg cgtaagtcta
     6121 tgatttgtcc gtttacttgg gcaaattcat cactcccagc gatagcaggg ttatctgttc
     6181 gtccgagcag gtagtcggtg gatacgttga aataattagc aatttctgcg atacgttcag
     6241 cgtttggcgt agaatttttt atcttataga gtgtatttct accataacct aagtcttctt
     6301 ctacttgtcc aagagctttt ccacgctttt ttgccaattc ttttattttt tcaaatgtct
     6361 caaacattgt taaatcaacc tttctaagac atcacaaaaa aaaatttaac aaatttggtg
     6421 taaaaaggtt gactaattat cccaaaaggt gtaaaatgtt ttttgtaagt gataaacaac
     6481 taaaaaaaca actaagaaat aaattataaa aatgttttgg cgaacggtat ttatagattt
     6541 attggtgttt ttattatgct ttaattttag ccgatttggt gtaagttgtc aagtataatg
     6601 cagaaaaata gttacatttt tagttgtttc ttatttacaa ataagtaaag aggaggaacg
     6661 tgctgatggt aagtattctt aaaaatttag aacaagaaaa agaccacctt gaaaaagtca
     6721 ttaaggtagt cagcgctggt ggtaaatttc tgagattgcc atatcaaaaa agtcacgctc
     6781 gattagtgag aatctgaaat tgatttctca aaatctttac catcatcgta gatgtcgcgt
     6841 ttgcttgctt tgagtaaata ttctttaaaa gtcataaggt taactccttt ctgcttatat
     6901 tatagcagaa aaagaagaaa gaaaaagaaa ggagaaaata tgccagatat cgcaaacggt
     6961 cgcgaaagag ttattgcttt cttgaaagag aaaggcatta aaaaagcaac tctagcggtt
     7021 gcttacggct ttaaacgaca ggaagtgaca aacattctaa gtggaacgac taaaggtcca
     7081 cgagcgaaca gtttcattct tcaggttatt gaagattatg ggattgagta ggaaagattt
     7141 gaggagtagg aaaatgagac caaaacgata tccgtatagt ggacaaaaag agtccacctt
     7201 tgtaaaggca gaccctgagt tagttgaaaa acttttaaga aacactagtt ttcttgagtg
     7261 tttacaaaaa aagcctatca attttcagat agactcagaa gaatttaagc gtcttagcta
     7321 tgaagccatt catgatactt ctcaagtaac tcaataggag gaaggaataa aatgattcac
     7381 cattatataa ctcactatgc cagcaatggg aaagattacg ccgaagcatg gattcaaatt
     7441 gattttttgg gaatgtgctt ttgtgtatgg aaaaagcgta caaccattga acgattgtac
     7501 gcaaacgaag attagacttt tttccaaccg ttgcctttag cagatgtcgg agggagccga
     7561 tcaccttttc cgatagttgc ggtatgacca ttagtaactt ttccgccacg aggtcctacc
     7621 tctacatagc gaccaggttt ctgattatct gttccaggtt ttattggagt atttgccata
     7681 ctatctcctc ctttctataa gtttgagaaa atcaacggta tctggtatta tttcgatggc
     7741 tcaggctata tgctttcaga ccgctggaag aagcacacag acggtaattg gtactacttt
     7801 gaccaatcag gcgaaatggc cacaggctgg aagaaaatcg ctgacaagtg gtactatttt
     7861 gatg
//
LOCUS       DAVMEI010000084         7496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVMEI010000084 DAVMEI010000000
VERSION     DAVMEI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.084"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..900
                     /locus_tag="VYQ24_001380"
     CDS             <1..900
                     /locus_tag="VYQ24_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1968511.1"
                     /translation="LGKVPQTIHTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRK
                     RSVKKSSLTKELKEKILHYHNQKFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDL
                     LYPRKGKALKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVL
                     TDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLSNVFSEEHIY
                     YAHPYASWERGTNENHNRLIRRWLPKRTKKMTPKEVAFIEKWINNYPKKCLDYKSPRE
                     DFWMANLNLKFSKMEIIFIKRFQ"
     gene            975..1175
                     /locus_tag="VYQ24_001381"
     CDS             975..1175
                     /locus_tag="VYQ24_001381"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1968512.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            1210..1926
                     /gene="atpB"
                     /locus_tag="VYQ24_001382"
     CDS             1210..1926
                     /gene="atpB"
                     /locus_tag="VYQ24_001382"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1968513.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            1940..2434
                     /gene="atpF"
                     /locus_tag="VYQ24_001383"
     CDS             1940..2434
                     /gene="atpF"
                     /locus_tag="VYQ24_001383"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1968514.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            2434..2970
                     /locus_tag="VYQ24_001384"
     CDS             2434..2970
                     /locus_tag="VYQ24_001384"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1968515.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            2985..4490
                     /locus_tag="VYQ24_001385"
     CDS             2985..4490
                     /locus_tag="VYQ24_001385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1968516.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVHFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            4506..5384
                     /locus_tag="VYQ24_001386"
     CDS             4506..5384
                     /locus_tag="VYQ24_001386"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1968517.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            5470..6876
                     /gene="atpD"
                     /locus_tag="VYQ24_001387"
     CDS             5470..6876
                     /gene="atpD"
                     /locus_tag="VYQ24_001387"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1968518.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            6887..7306
                     /locus_tag="VYQ24_001388"
     CDS             6887..7306
                     /locus_tag="VYQ24_001388"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1968519.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDNKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(7348..>7496)
                     /locus_tag="VYQ24_001389"
     CDS             complement(7348..>7496)
                     /locus_tag="VYQ24_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1968520.1"
                     /translation="AKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFV
                     LSRA"
BASE COUNT         2261 a         1411 c         1645 g         2179 t
ORIGIN      
        1 cttggaaaag ttcctcaaac tatccacact gaaatcaagc gtgggacagt ccgacaatgt
       61 cttggaaaag ggcgcttcaa agaggtttat tctgccgact atgctcaaca gtcttatgaa
      121 aataatcgca agcgctcggt caagaaatca agcttgacca aggaactaaa ggaaaagatt
      181 ctccactatc ataaccaaaa attttcgcct gaaatgatgg tgatggctaa aggggttaac
      241 gtgggaattt caaccattta ctattggatt catcatggaa aattggggtt aagcaaacag
      301 gatctgcttt accctagaaa aggaaaagcg cttaagaaac aggctagcac caactttaaa
      361 cctgctggtc aatccatcga acagcggtct gaagctatca atcttcgctt ggagaatggg
      421 cattatgaga ttgatacggt tctacttacg agagcgaaaa actactgctt gcttgtcttg
      481 acggatcgaa aaagtagaca tcagatcatc cgattgattc caaataaaag tgctgaggtg
      541 gtcaatcagg ctctaaaact catcttaaaa caacacaaga ttctttccat cacggcagat
      601 aatggaacgg aattcaatcg cttgtctaat gtattttctg aggagcacat ttattatgca
      661 catccctatg cctcttggga aaggggaact aatgagaatc acaacaggct cattcgtaga
      721 tggttaccta agagaaccaa gaaaatgact cccaaagaag tcgcattcat cgaaaagtgg
      781 attaacaact atcctaaaaa atgcttggac tacaagtcac ccagagaaga cttctggatg
      841 gctaacttga acttgaaatt tagcaaaatg gaaataattt ttattaaacg cttccaatag
      901 cggttaaaag ttgacaaatt taaattttag gactacacta agtaagtaaa ttttatttta
      961 aaggagaatt tgttatgaat ttaacatttt taggcttatg tattgcctgt atgggcgtat
     1021 ctgtcggtga aggtttattg atgaatggac tgtttaaatc agtagcacgc caaccagata
     1081 tgctttctga gtttcgtagt ttgatgtttt taggtgttgc ctttattgaa ggaactttct
     1141 ttgtaactct tgtcttctca tttattatca aataaataca tggaacgaga agaaaaggga
     1201 ggattttaga tggaagaaag tattaatcca atcatctcta ttggtcctgt tatcttcaat
     1261 ctgactatgt tagccatgac tttgttgatt gtgggagtta tttttgtctt tatttattgg
     1321 gcaagccgca atatgacctt gaaacccaaa ggaaagcaaa atgtacttga gtatgtctat
     1381 gactttgtta ttggatttac agaacctaac attggttcgc gctacatgaa agattactca
     1441 ctctttttcc tttgtttatt ccttttcatg gtgattgcca ataaccttgg cttaatgaca
     1501 aagattcaaa cgatcgatgg gactaactgg tggagttcgc caaccgctaa tttacagtat
     1561 gacttaacct tatcttttct tgtcattttg ttgacacata tagaaagcgt tcgtcgtcgt
     1621 ggatttaaaa aaagtataaa atcttttatg agtcctgttt ttgtcatacc gatgaatatc
     1681 ttggaagaat ttacaaactt cttatctttg gctttgcgga tttttgggaa tatctttgca
     1741 ggagaggtca tgacgagttt gttacttctt ctttcccacc aagctattta ttggtatcca
     1801 gtagcctttg gagctaattt ggcttggact gcattttctg tctttatttc ctgcatccaa
     1861 gcttatgttt ttactctttt gacatctgtg tatttaggga ataagattaa tattgaagag
     1921 gaatagaaag gagtaactga tgcacgtaac agtaggtgaa ttaattggta attttgtttt
     1981 aatcactggc tcttttattc ttttgctagt cttgattaaa aaatttgcat ggtctaatat
     2041 tacaggcatt ttcgaagaaa gagctgaaaa aattgcttca gatattgaca gagctgaaga
     2101 agcccgtcaa aaagcagaag tattggctca aaaacgcgaa gatgaattgg ctggtagccg
     2161 taaagaagct aagacaatca ttgaaaatgc aaaggaaaca gctgagcaaa gtaaggctaa
     2221 tatcttagca gatgctaaac tagaagcagg acacttaaaa gaaaaagcca atcaagaaat
     2281 tgctcaaaat aaagtagaag ctttacagag tgttaagggt gaggtcgcag atttgaccat
     2341 cagcttagct ggtaaaatca tctcacaaaa ccttgacagt catgcccata aagcactcat
     2401 tgatcagtat atcgatcagc taggagaagc ttaatggaca agaaaacagt aaaggtaatt
     2461 gaaaaataca gcatgccttt tgtccaattg gtacttgaaa aaggagaaga agaccgtatc
     2521 ttttcagact tgactcaaat caagcaagtt gttgaaaaaa caggtctgcc ttctttttta
     2581 aaacaagtgg cagtagacga gtcggataag gaaaaaacaa ttgctttttt ccaagattct
     2641 gtgtcacctt tattacaaaa ctttatccag gttctggcct acaatcacag agcaaatctt
     2701 ttttatgatg tgcttgtaga ttgcttgaac cgacttgaaa aagaaacaaa tcgatttgaa
     2761 gtgacgatta cgtctgctca tcctctaact gatgaacaga agactcgttt gctccctttg
     2821 attgagaaaa aaatgtctct gaaagtaagg agtgtaaaag aacaaatcga tgaaagtctc
     2881 attggtggtt ttgtcatttt tgccaatcac aagacaattg atgtgagtat taaacaacaa
     2941 cttaaagttg ttaaagaaaa tttgaaatag aaagtggtgt tcttttggca attaacgcac
     3001 aagaaatcag cgctttaatt aagcaacaaa ttgaaaattt caaacccaat tttgatgtga
     3061 ctgaaacagg tgttgtaacc tatatcgggg acggtatcgc gcgtgctcac ggccttgaaa
     3121 atgtcatgag tggagagttg ttgaattttg aaaacggctc ttatggtatg gctcaaaact
     3181 tggagtcaac agacgttggt attatcatcc taggtgactt tacagatatc cgtgaaggcg
     3241 atacaatccg ccgtacaggg aaaatcatgg aagtccctgt aggtgaaagt ctgattggtc
     3301 gtgttgtgga tccgcttggt cgtccagttg acggtcttgg agaaatccac actgataaaa
     3361 ctcgtccagt agaagcacca gctcctggtg ttatgcaacg taagtctgtt tcagaaccat
     3421 tgcaaactgg tttgaaagct attgacgccc ttgtaccgat tggtcgtggt caacgtgagt
     3481 tgattatcgg tgaccgtcag acagggaaaa caaccattgc gattgataca atcttgaacc
     3541 aaaaagatca agatatgatc tgtatctacg tcgcgattgg acaaaaagaa tcaacagttc
     3601 gtacgcaagt agaaacactt cgtcagtacg gtgccttgga ctacacaatc gttgtgacag
     3661 cctctgcttc acaaccatct ccattgctct tcctagctcc ttatgctggg gttgctatgg
     3721 cggaagaatt tatgtatcaa ggtaagcatg ttttgattgt atacgatgat ctatcaaaac
     3781 aagcggtagc ttatcgtgaa ctgtcgctct tgcttcgtcg tcctccaggt cgtgaagcct
     3841 tcccagggga tgttttctat ctccacagcc gtttgcttga gcgctcagct aaagtttctg
     3901 atgaacttgg tggtggatca attacagccc taccatttat cgagacacaa gcaggagata
     3961 tctcagccta tatcgcaacc aacgtgattt ctatcactga tggacaaatc ttccttggcg
     4021 atggcctctt caatgcaggt attcgtccag ccatcgatgc gggttcatct gtatctcgtg
     4081 taggtggttc tgcacaaatc aaagccatga agaaggttgc tggtacactt cgtatcgacc
     4141 ttgcttcata ccgtgagttg gaagccttta ctaagtttgg ttctgacttg gacgcagcaa
     4201 cacaggctaa gttgaaccgt ggacgtcgta ccgttgaggt cttgaaacaa cctgttcaca
     4261 aaccattacc tgttgagaaa caagtaacca ttctttatgc tttgacacat ggtttcttgg
     4321 atactgttcc agtagatgat attgttcatt tcgaggaaga gttccatacc ttctttgatg
     4381 ctcaacatcc agagattttg gaaaccattc gtgatacaaa agacttgcca gaagaagcag
     4441 tcttggatgc tgcgattaca gagtttctca atcaatctag cttccaataa gaatagaggt
     4501 gtcagatggc agtatctcta aatgatatta aaacaaaaat cgcctcaaca aaaaatacga
     4561 gtcaaatcac taatgccatg caaatggtat cggctgctaa gctaggtcgt tctgaagaag
     4621 ctgctcgcaa cttccaagtt tacgctcaga aagtgcgtaa acttttgaca gatatccttc
     4681 atggtaatgg agctggtgct tcaactaatc cgatgttgat tagccgttct gtgaagaaga
     4741 caggctatat cgttatcact tcagaccgcg gtttggttgg aggttataat tcctctattt
     4801 tgaaagctgt tatggagttg aaagaagaat accacccaga cggtaaaggt tttgaaatga
     4861 tctgtatcgg tgggatggga gctgatttct ttaaggctcg cggtattcaa ccactttatg
     4921 aattacgtgg cttggcagac caacctagct ttgatcaagt tcgtaagatt atttcaaaaa
     4981 ctgttgaaat gtaccaaaat gaactctttg atgagcttta tgtttgctac aaccaccatg
     5041 tcaatacgct aaccagtcaa atgcgtgtgg aacaaatgct tccgattgtt gacttggatc
     5101 caaatgaagc ggatgaagag tacagcttga cttttgaatt ggaaaccagc cgagaagaaa
     5161 ttctggagca gttgttgcct cagtttgcag aaagtatgat ttacggtgcc attatcgatg
     5221 ccaagacagc tgagaatgct gcgggtatga cagccatgca aacagcgaca gataatgcta
     5281 agaaagtcat caatgatttg acaattcagt ataaccgtgc cagacaggcg gcgattacac
     5341 aagaaattac agaaatcgta gcaggtgcta gtgccttaga ataggctcta gtccagctcg
     5401 tatgaaaatg aacttaggac ctagttgagc taggaaccga cagtatctta tatagaatag
     5461 gagaaggaga tgagttcagg taaaattgct caggttatcg gtcccgttgt agacgttttg
     5521 tttgcagcag gggaaaaact tcctgagatt aacaatgcac ttgtcgtcta caaaaatgac
     5581 gaaagaaaaa caaaaatcgt ccttgaagta gccttggagt taggagatgg tatggttcgt
     5641 actatcgcca tggaatcaac agatgggttg actcgtggaa tggaagtatt ggacacaggt
     5701 cgtccaatct ctgtaccagt aggtaaagaa actttgggac gtgtcttcaa cgttttggga
     5761 gataccattg atttggaagc tccttttaca gaagacgcag agcgtcagcc aattcataaa
     5821 aaagctccaa cttttgatga gttgtctacc tcttctgaaa tccttgaaac agggatcaag
     5881 gttattgacc ttcttgcccc ttaccttaaa ggtggtaaag ttggactttt cggtggtgcc
     5941 ggagttggta aaaccgtctt aatccaagaa ttgattcaca acattgccca agagcacggt
     6001 ggtatttcag tatttactgg tgttggggaa cgtactcgtg aggggaatga cctttactgg
     6061 gaaatgaaag aatcaggcgt tatcgagaaa acagccatgg tctttggtca gatgaatgag
     6121 ccaccaggag cacgtatgcg tgttgccctt actggtttga caatcgctga atacttccgt
     6181 gatgtggaag gccaagacgt gcttctcttt atcgataata tcttccgttt cactcaggct
     6241 ggttcagaag tatctgccct tttgggtcgt atgccatcag ccgttggtta ccaaccaaca
     6301 cttgctacgg aaatgggtca attgcaagaa cgtatcacat caaccaagaa gggttctgta
     6361 acctctatcc aggctatcta tgtgccagcg gatgactata ctgacccagc gccagcaaca
     6421 gccttcgctc acttggattc aacaacaaac ttggaacgta aattggtaca attgggtatc
     6481 tacccagccg ttgacccact tgcttcaagc tcacgtgcct tggcacctga aatcgttgga
     6541 gaagagcact atgcagttgc tgctgaagta aaacgtgtcc ttcaacgtta ccatgaattg
     6601 caagatatca ttgctatcct tggtatggat gagctttctg atgaagaaaa gaccttggtt
     6661 gctcgcgccc gtcgtatcca gttcttcttg tcacaaaact tcaacgttgc ggaacaattt
     6721 actggtcagc caggttctta tgttccagtt gctgaaactg tacgtggctt taaggaaatc
     6781 cttgatggta aatacgacca cttgccagaa gatgccttcc gtggtgtagg ttctatcgaa
     6841 gatgtgattg caaaagctga aaaaatggga ttttaagagg tgatctatgg ctcagttaac
     6901 tgtccagatc gtgacaccag atggtctcgt ctatgatcac catgccagct atgtatcggt
     6961 tcgaactctg gatggtgaga tggggatctt gccacgacat gaaaatatga ttgcagtttt
     7021 agcagttgat gaagtaaagg taaaacgtat cgataataaa gatcacgtga actggattgc
     7081 agtaaacggt ggcgttattg aaattgccaa tgatatgatc acaatcgtcg ctgactctgc
     7141 agaacgtgct cgtgatatcg atatcagtcg tgcagaacgt gccaaacttc gtgcagaacg
     7201 tgcaattgaa gaagcacaag acaaacattt gattgaccaa gaacgtcgtg ctaagattgc
     7261 tttgcaacgt gctattaacc gtattaatgt cggaaataga ctataagaaa aaatggctct
     7321 ttgtcaactg tagtgggttg aagtcagcta agctcgagaa aggacaaatt ttgtcctttc
     7381 ttttttgata ttcagagcga taaaaatccg ttttttgaag ttttcaaagt tccgaaaacc
     7441 aaaggcattg cgcttgataa gtttgatgag attattggtc gcttccaatt tggcgt
//
LOCUS       DAVMEI010000085         7258 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVMEI010000085 DAVMEI010000000
VERSION     DAVMEI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.085"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(80..160)
                     /locus_tag="VYQ24_001390"
                     /pseudo
     CDS             complement(80..160)
                     /locus_tag="VYQ24_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(204..2201)
                     /gene="metG"
                     /locus_tag="VYQ24_001391"
     CDS             complement(204..2201)
                     /gene="metG"
                     /locus_tag="VYQ24_001391"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1968521.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPEGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHAHMEAVDYPRALEAVWTLISRTNKYIDETAPWILAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2325..3584)
                     /locus_tag="VYQ24_001392"
     CDS             complement(2325..3584)
                     /locus_tag="VYQ24_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968522.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3586..4287)
                     /locus_tag="VYQ24_001393"
     CDS             complement(3586..4287)
                     /locus_tag="VYQ24_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968523.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4271..5470)
                     /locus_tag="VYQ24_001394"
     CDS             complement(4271..5470)
                     /locus_tag="VYQ24_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1968524.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5735..7081
                     /gene="gor"
                     /locus_tag="VYQ24_001395"
     CDS             5735..7081
                     /gene="gor"
                     /locus_tag="VYQ24_001395"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1968525.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         2112 a         1608 c         1366 g         2172 t
ORIGIN      
        1 tcgccctacc gtatgtatgg ttactgactt cgtcagtttc atctacaacc tcaaaacagt
       61 gttttgagct gacttcgtca gtttcatcta caacctcaaa gcagtgcttt gagcaacctg
      121 cggctagctt cctagtttgc tctttgattt tcattgagta taaataaaaa aacagaacta
      181 gtctgaacta gtcctgtcta cttttaccca atcacacttc catttggtac agctggatca
      241 actgtgagaa gggttaattt gccatcatgt tcagctgata gaatcatacc ctggctgaca
      301 tattttttca tcattttacg tggtttgagg ttagcaacga tttgaacttt cttgccgacc
      361 aattcttgtt catttggata gtattttgca attcctgaaa gaatctgacg atcttctcca
      421 tcaccagcat ccaagcggaa ttgaagcaac ttatctgaac cttctacttt agacacttct
      481 ttgacttctg ccacacggat ttcaaccttg tcaaagtctt caaacttgat ttcatccttg
      541 tttagtttga gctcaacttc gtccggattc cattcttttt cgactgctgg tttattgcct
      601 tccatttgtt ccttgatata ggcgatttct tcttccatat ttagacgtgg aaagataggt
      661 gttcctttgg caactacagt cacatctgct gggaagtcag ccaaactcaa gttttcaaga
      721 ctagaaactt cttccaaacc aagttgagtc aaaactgcac gactagtttc catcataaat
      781 ggttcaatca agtgagcaac tacacgaatg ctggctgcca agtggctcat gacacttgcc
      841 aattggtcac gaagagcttc atccttggcc aagatccatg gtgcggtctc atcgatgtat
      901 ttattggtac gagagatcag agtccagact gcttcaagcg cacgtggata gtcaactgct
      961 tccatgtgtg catggaagtc tgcgattgat tgttctgcaa cctcagcaag aacatggtca
     1021 aattcagtca caccttctac ataggcaggg atttgtccat caaagtactt attaatcatg
     1081 gaaaccgtac ggttaaggag gttcccaagg tcattagcca attcatagtt gatacggccg
     1141 acatagtctt caggagtaaa ggttccgtct gaaccaactg gaaggttacg catgaggtag
     1201 taacgaagtg gatctagtcc ataacgctct accaacattt cagggtaaac gacattccct
     1261 tttgacttag acatttttcc gtctttcatg acaaaccaac catgggcaat caaacgatca
     1321 ggtaatttaa catccaacat cataagaagg attggccagt agatagagtg gaagcgaagg
     1381 atgtcttttc ctaccatatg gaagactgtt ccattccaga acttgtcaaa gttaccatgt
     1441 tcgtcttgag cgtagccaag agctgtcgca tagttaagaa gggcatcaat ccaaacgtag
     1501 acaacgtgtt ttggatttga tgggacaggc actccccatg taaaggttgt acgagatacc
     1561 gccaaatctt ccaaacctgg ctcgatgaag ttgcgtagca tttcattaag gcgaccctct
     1621 ggcgtgataa attcaggatg agctttgaaa aattcgacca aacggtcttg gtatttgcta
     1681 aggcgaagga agtatgattc ttcagaaacc cattcaacct catgacctga tggagcaata
     1741 ccaccagtca catttccagc ttcatcacgg aaaacttctg ccagctggct ttctgtaaag
     1801 aattcttcgt ctgatactga ataccaacca gagtattcac ccaagtagat atcatcttga
     1861 gcaagtaagc gttcaaagac ctgtgcaaca actttttcat ggtagtcatc agttgtacgg
     1921 ataaatttat cgtatgagat atctagtaat tgccagagtt ctttaactcc aaccgccatt
     1981 ccatcaacat aggcttgagg tgtaatacca gcttcttccg ctttctgctg gattttctga
     2041 ccatgttcat caagacctgt cagataaaat acatcgtagc ccatcaggcg tttgtaacgt
     2101 gctaggacat cacatgcgat agttgtgtag gcagaaccga tatgaagttt cccagatgga
     2161 tagtaaatcg gcgttgtaat ataaaaattt ttttcagaca taatttttcc tttccaggca
     2221 aatgaaacct gtttttctaa cacttcatta tatcacattt ttaatgaatt tcgataggga
     2281 aatccataca aaaacaagat agacgagtgt ccatcttgtt gatctcattc ataacgaagg
     2341 gcttcaattg gatcaagttt cgatgccttg ttggctggca agactccaaa aatcatacca
     2401 acactagccg aaactgcaag actaaatagg gcaactggga ttgatactcc aacttctata
     2461 ccttctatta aaccttgcag taacaaacct gctaaggcag ttaaaccact tgcaattgtc
     2521 aagccaatta agccacctaa caaggtcaaa atcatggatt caatcaaaaa ctgaattaaa
     2581 atattggcac gtgttgcgcc caaagcctta cgaagaccaa tctcacgagt gcgctctgtc
     2641 accgaaacca gcatgatgtt catgacacca gttcctccaa caaagagaga aatccctgcg
     2701 atggaactaa taatcgtcgt cataaaacta aacgattgtt gaatttctgc aaatacaacg
     2761 gactcatctg ccacctggta ttctccctgt tgtaagcctg caagctctgt catttttcgt
     2821 gccagttctg gacccagagt tggggttaaa ctggtatcat tcactcgaaa gacaatacta
     2881 gctatttcat ctatattaaa attcgcagca agggagatat tggtagtaat aggcaagcca
     2941 ccaaacccat atatttttga ccttttagcc tccggactag tataaacccc aatgacccgg
     3001 taactaaatc cattgacttc tacaaccttg ttaatagcct cttgaggaga ttcaaataaa
     3061 ctaatggaca attcctcatc tagcaaaata acacttgcaa actctttgaa atcttgctct
     3121 ctcagactac gacctgcaat aatttcattc ttaacagcgt ccatgtaagt tctgtttcca
     3181 cctgtcaaat tagcattctc aaccttttta tcttgatagg tcaagatggc attcgttgaa
     3241 ttggttacat agtaactatc cactcccttc agtttagctg cctcttggac ccaggattct
     3301 tgcggttttg gcggttcaac aggaatttcc tcttcctttc cagaaaccgt aaaagatgat
     3361 tgtttctgag taaaagaacc atctttactt tttttaggag agaaaaagac gctaatattt
     3421 ttctgagatt tagtcatatc tttattgact tgacgagata gggaatcacc caaagccata
     3481 atcacaacaa ctgatgaaac accgataata atcccaatca tagtaagcaa agaacgcatc
     3541 ttgtgagcca tgatagatga aaaggcaaat ttcagattct gcatcttagt tttcctcctt
     3601 tcctaactga gcactgtcag acgaaatgac cccatcccga atgacaatct gacgtttagc
     3661 ataggcagca atctcaggct catgcgttac catgataatg gtttttcctt ctttattcaa
     3721 atcaaccaat aattgcataa tttggttacc tgttttggta tccaaggctc ctgtcggttc
     3781 atccgctagg ataatagaag gattgtttac caaggcacgc gcaatggcta cacgttgctt
     3841 ttgaccacca gataattctg aaggtaaatg gtgactacgt tctgtcaatt caaccttgtc
     3901 taaatattcc tcagccaact tgcgacgttt tgaagacgaa actcctgcgt aaatcaaggg
     3961 caattctaca ttttgcagag cattgagctt cgatagaaga aagaactgct gaaagacaaa
     4021 accgatttgt tggttacgga ccttagccag ttgtttttca ccaagcccag ccacttcttg
     4081 accttcaaga taatattctc cactggttgg tgtatccaac atgccaatcg tattcatcag
     4141 agtggactta ccagacccag atggtcccat gatggctaca aattcaccct cattcacttc
     4201 tagattgata tttttgagaa cctgcagttc ttggtcacca ttacggtagc ttctgaagat
     4261 attttttaga ctaattagtt gcttcatcag ccttcacctc ttttccttct tccaaggaag
     4321 atgttggatt actgatgacc ttagcaccat tcgttaaacc agaagtgatt tcttgatttt
     4381 ctgcgtcagc atttcccaat gaaacctcaa cttttttagc cttttgttgt tcatccacaa
     4441 tccagacata atttttacta tcatccatta ctagactgct aacaggaaca agaatagcct
     4501 tagttttgct tttaacctca atgttgacag aaaaaccctg tttcaaatca ccaacctcgc
     4561 ctgtcacatc aatagtataa gggtatttag aacctgtatt attcccggct gctggactag
     4621 ctgcttcacc attgttttta ggatagtcag aaatatagct taatttccca gtccattttt
     4681 tatcaggata cactttagaa gtaaagctta cttcttgacc tacagaaagg ttggctagat
     4741 tgtactcaga caattctccc ttgacttgta aattttcatt gctgacaata tgaaccataa
     4801 cttggctcgc ccctgttgga gatttagaaa cattgctatt gacttcgact acagttccct
     4861 ctagggtact gagaacagtt gttgcatcca attgactttg agccttgctt aattgcgctg
     4921 cagcatctgc acgcgcatca cgggcatcac ccaattgagc gtcaatagaa gcaacagaat
     4981 ttccagccac tggagttggg ctttgcaccg ttgcatcttc tcctcctact ggcgctggta
     5041 actgtggagc cggagctgaa gcggcttcat ttcgtgcttg attgagttca ttgatatgac
     5101 gatctgcctt agctactgct cgactagctg aatcataggc cgcctgcgct tctgaactac
     5161 tgtacttgac taaagcctgc ccttcgctga ccttatcgcc cacagaaaca aggatttcat
     5221 ctaaatcacc cttactagca tcaaaataaa catattgttc attttttgct gttactgtcc
     5281 ctgacaataa aacagaggag gccacgcttc cttccttggc aacaacaaga tgagtaggct
     5341 catcttttag agcagtctga gaaggttgtc taaagagtaa aatcccccca gcacccaata
     5401 caactacact cgcagcaccg attgctgcat acagttgcca ctttttagct ttaccattct
     5461 ttttcttcat aatgaaactc cttttctttt ttacaatact ttgctattat accaaatttc
     5521 cctccagcaa acaatacagt tcaggattaa acaatcgttc ggaattttgc ttttctattg
     5581 tactagatat ataatacact atcttgactt atttggcaag cttttttgca attttttgtg
     5641 cgtagcagat ttttgcaatc aaatcaaaaa aagaataaaa tataactatc aaaaaagacg
     5701 ctctactatt taaaagatta caaaggagtt ttcaatgaga gaatatgata tcattgctat
     5761 cggtggaggt agcggaggaa tcgctaccat gaaccgtgct ggtgaacatg gagccaaagc
     5821 agccgttatt gaggaaaaga aattaggtgg aacctgtgtc aacgtcggtt gtgttcctaa
     5881 aaaaatcatg tggtacgggg cgcaaatcgc tgagactttc catcaatttg gagaagacta
     5941 cggctttaag actactgatc ttaactttga ctttgcaacc ctacgtcgca atcgtgaagc
     6001 ctacattgat cgcgctcgtt cttcttatga tggtagtttt aaacgcaacg gtgtagactt
     6061 gattgaaggt catgctgaat ttgtagattc tcatactgta agcgtaaatg gtgaactgat
     6121 tcgtgctaaa catatcgtga ttgctacagg tgcccatcca agtattccta atattcctgg
     6181 tgctgagcta ggtggctctt ctgatgatgt atttgcctgg gaagaacttc cagagtcaat
     6241 tgccattcta ggcgctggtt atatcgccgt tgaattagct ggcgtactcc acacttttgg
     6301 tgtcaagaca gatctctttg ttcgccgcga tcgtccttta cgtggttttg attcctacat
     6361 cgttgaaggt ttggtcaagg aaatggaaag aacaaactta ccacttcaca ctcacaaagt
     6421 ccctgtcaag ttagaaaaaa ctactgacgg cattaccatt catttcgaag atggtactag
     6481 tcacacagct agccaagtta tctgggctac aggtcgccgt ccaaacgtta agggcttgca
     6541 acttgaaaaa gctggagtga ctctgaacga acgtggcttt atccaagtgg atgaatacca
     6601 aaatactgtt gttgagggaa tctatgctct aggtgatgta acgggcgaga aagaactgac
     6661 tccagttgca atcaaggccg gacgtaccct atctgaacgt ctctttaacg gaaaaactac
     6721 tgcaaaaatg gattactcaa ctattccaac tgttgtcttt tcacaccctg ctatcggaac
     6781 tgttggtttg acagaagagc aagctattaa agaatacggt caagaccaaa tcaaggttta
     6841 taaatcaagc tttgcatcta tgtactctgc ttgcactcgc aaccgtcaag aatcccgttt
     6901 caaactcata acagctggtt cagaagaaaa agttgtcgga cttcatggaa ttggctacgg
     6961 cgttgatgaa atgattcaag gatttgccgt tgctatcaaa atgggagcaa ccaaggctga
     7021 ctttgatgca actgtggcaa ttcacccaac tgcatctgaa gaatttgtaa ccatgcgtta
     7081 atcgaaacaa aagaagcaaa gcaaaaaaca cttctaaata tcaaaaagcg agtatttccg
     7141 tagttggaga tactcgtttt cttatgtttt attttatagt atgttgaagt aagatatgca
     7201 aaaatcaatt aacaaaccat agcaaaatac aaggattata atacaatcat ttattctt
//
LOCUS       DAVMEI010000086         7192 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVMEI010000086 DAVMEI010000000
VERSION     DAVMEI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.086"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..227)
                     /locus_tag="VYQ24_001396"
     CDS             complement(<1..227)
                     /locus_tag="VYQ24_001396"
                     /inference="COORDINATES: protein motif:HMM:NF020296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1968526.1"
                     /translation="MIDWDFADTCWEKDPREYQYVAANYLKAMQSYLKETDLPKLEQL
                     VVTKSWWDTVDILDRVVGSLVYEHSELEEII"
     gene            complement(149..390)
                     /locus_tag="VYQ24_001397"
                     /pseudo
     CDS             complement(149..390)
                     /locus_tag="VYQ24_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(391..1023)
                     /locus_tag="VYQ24_001398"
     CDS             complement(391..1023)
                     /locus_tag="VYQ24_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1968527.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(1037..2032)
                     /locus_tag="VYQ24_001399"
     CDS             complement(1037..2032)
                     /locus_tag="VYQ24_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1968528.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FFFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(2029..2727)
                     /locus_tag="VYQ24_001400"
     CDS             complement(2029..2727)
                     /locus_tag="VYQ24_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1968529.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(2804..3814)
                     /locus_tag="VYQ24_001401"
     CDS             complement(2804..3814)
                     /locus_tag="VYQ24_001401"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1968530.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(3798..4805)
                     /locus_tag="VYQ24_001402"
     CDS             complement(3798..4805)
                     /locus_tag="VYQ24_001402"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1968531.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRADIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFFAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(4792..5745)
                     /gene="mvaD"
                     /locus_tag="VYQ24_001403"
     CDS             complement(4792..5745)
                     /gene="mvaD"
                     /locus_tag="VYQ24_001403"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1968532.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(5727..6605)
                     /gene="mvk"
                     /locus_tag="VYQ24_001404"
     CDS             complement(5727..6605)
                     /gene="mvk"
                     /locus_tag="VYQ24_001404"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1968533.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLSHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(6727..7056)
                     /locus_tag="VYQ24_001405"
     CDS             complement(6727..7056)
                     /locus_tag="VYQ24_001405"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1968534.1"
                     /translation="MIRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVR"
BASE COUNT         2063 a         1745 c         1345 g         2039 t
ORIGIN      
        1 aagattattt cttctagttc agagtgttca tacaccaaac tccctactac tcgatctagg
       61 atatctaccg tgtcccacca ggattttgtc acgactaact gctctagctt aggcaaatcg
      121 gtttccttta gataagactg cattgctttc aaatagttag cagccacata ttggtattct
      181 ctaggatcct tttcccagca agtgtctgca aaatcccaat cgataatctt tgtttttttc
      241 gcttctggaa aatattttta tagagtttat ttctttcagg caccgcaata cctagaaaag
      301 aaaattgatg gcgcatatag gcttccatgg gccttgcttt ttagagtctt ttgctgcttc
      361 tagctcctca agtaaatctg ctaaactcat ctaaaactcc tcatgcccca ccaaatggtg
      421 ctgaaaggca tagacagccg cctgggtacg atcgctgact tcaagtttgg caagaatgtt
      481 ggacacgtgg gtcttgaccg tcttgagaga gataaagagg tcatctgcga tgcgctgatt
      541 ttcgtagccc ttggcgatga gttggagaac atctcgctca cgcgcagtca attcttcatg
      601 aagttccata tgattgcggt ggtattcaac cttcttgcta acctcttgct caatggccag
      661 ctcgccagca gctaccttac tgacggcatg aagcaattca tctgcactag aagtcttgag
      721 catatagcct ttggcaccag catctaagac tggcatgatt ttttcattgt ccaaataaga
      781 ggtcacaatc aaaatcttgg cttcaggcca ttctttaagg attgctaagg tcgcgtcaat
      841 cccattcatc tcaggcatga caatatccat gacaatgaca tctggacgca gttccaaggc
      901 caagtcaatc ccttgagacc cgttggacgc ctcacctaca acttctacat cgtcttggag
      961 gtcaaagtag cttttcaagc ccaatcggac catttcatgg tcatctacta gtaaaatttt
     1021 catctttact cctttatcat tccttatcta acaggggaat acggatatca accgccagcc
     1081 cttgcttggg agctgtcaag agttgaactg ttccagccat atcttcaacc cgctccttga
     1141 tatttcgcag tccataactc aagtcgtcta agctccctaa ctggaaacca atcccattgt
     1201 ccaccacctt cagttgcaat tcaacatctg tctgatagag gtagacatct aggcaagatg
     1261 cctgggcatg gcggagcgta ttgctaatca actcttgcag gatacggaag atatgctcct
     1321 cgattttctt aggcaatttc gtcatattct gcttgagact aaccctaaga tcactcttgt
     1381 cctcaagctc ttttaaaaga atttgaatcc cttctatcaa gctcttctgc tccagttcaa
     1441 ctggtcgcaa atgcaagagc aaaacccgca aatccttctg ggctgtttct aaaatagctg
     1501 tgacactctg caactgggtc tgcatctttt ctctatccaa tttcaaagcc tgctgactga
     1561 tacccgataa aatcatgtgg gccgcaaaca actcctgact gactgtatcg tgcaaatccc
     1621 gagcaattcg cttccgttcc ttctcgatga tttcctcttc ctgagcaagg ctatgatttt
     1681 cagctttttg aagagcctct gtcaaaaggt taagtttacc tgataaggac ttgaaactgg
     1741 catccaaatc tggatctaca acctgaacca cttcttgccc tgccaataaa cgcttgagat
     1801 tagcctgcat ttttcttaga gaaagctctt cgatccctcg ccaaaacagg gctaagagac
     1861 aggtcatgga catgctgaaa accaacaata aaaagaaaaa tttttctgtt ttttcgacat
     1921 cgtgcaaaaa gatagaccag tcaaaatcaa gtatttccag caagctatgg gagaaaaaaa
     1981 agacaaacag gaaggaggtg agagcgataa tgacataggc ttgttttttc atcctctaac
     2041 cacctcaaca tcaccaatca tagtggtcaa gaaaatcttg acactcttgt tactcttgag
     2101 atagtctttt gtttcttgat gatagtgttc attgcggagg gctcgcttgg gctggttgaa
     2161 aaaagtcaaa tccccataga gacagttaac gctgagactg acttccacat ctacaggtac
     2221 gatgattttg gtcgttccta ccatctttct gaggataatg acattgtcat gattggttaa
     2281 gatgaccctc tccagatgaa tagtgtcctt gcccatgaag cgaaagagat tgatatcatc
     2341 aaattggcaa gtctggtagc ttgaaaaatg atgaagattt ccaaaccaac gatttttctc
     2401 cttcttaacc gtcacaacct cttcaaaaac caaattggtc tgctcttttt cctggttcat
     2461 catcgggtaa agaagaaaga gactatagat aaccgcaaca aaaatagcta gaatcacaaa
     2521 aggattgagc ataacgatga aaaagaagag aatggttgcc gctactaaaa gaagattatt
     2581 cccctcttta ccagtgtagt agcgaatcaa aagcaaaaag aggaatagta tcagcagaaa
     2641 acgcgaaaaa tgctctgata ccatcaaaat cagagctcct gtcagaagac aggcttcgat
     2701 aaataaaaag attttaaatt ttctcatagg ttcatcctct cccttctatt ttatcacaat
     2761 tcaaaaaagt cacctcagtc tgaggatgga aaaaaggcgc tggttacgcc tttttcatct
     2821 gatcctttgc ttcttttaat tttccataaa gaagatagtc tactttttgt agatctgcta
     2881 tggtggcaca gttaagggaa cacataatca agcgtaggtc tgctttccag ccttggacaa
     2941 tgccaatcac ttcttcaact gtgtaggttt caaccaattc cagaacggtt cgtgacaatc
     3001 ccacagactt agcaccaaaa accaagcact taatcatatc cagcggattc cgaacccctc
     3061 cactaaccaa gagttcgacc ttatctttcc attcttgggc attgagaagg gcctgcatgg
     3121 tagactgacc ccattgattg aggtaatcac gctggccact acgacggttt tcgatatagg
     3181 caaagctggt gccaccacga cccgataggt ccactgtacg aacaccgaat tcataggctc
     3241 tttcgattgt cttggcatcc attccaaagc ccacttcctt gaggacaata ggaacgggaa
     3301 tttgctcgct ataatctgct agatgcgatt gccagcttct aaacttcctt tctccctcgg
     3361 gcatgagtaa ttcctgcatg acattgacat gcacttgcaa tagaacagga ttcatctctt
     3421 ctacagtctg aagtcctaac tcgacaggct tgtccaatcc aatattggtt ccaaggagga
     3481 gattgggatg actagacttg acagaaaaag aatcatccgt tggatttttg agggctgcgc
     3541 tataagaacc cgttacaaat aaaataccac aggattccgc cacctgagcc agcttttgat
     3601 tgatttctct tcccttatta cttccaccag tcatggcatt gatataaaaa ggaaagtccc
     3661 actttcgacc agcaaactct gtcgaaagat cgatttcatc cagattgtaa agaggcaagg
     3721 aagaatgaat cagctccacc tcatcaaagc tattatagga acttttctgc tcaagggcat
     3781 agaggatatg ctcgtcctta cgatttgtcg tcatgtccta tcctttcttg atataagagc
     3841 tcaatcccca gatcggccca acgatttttt aaggtttcgg ttgattgcgc atcaaaactc
     3901 aaggcgatgc cacagtcacc accaccagca ccactactct tggcaacggc ctgcaaatct
     3961 tgactggctt ctttcaactg tctaagcaaa ggcgtgtaaa tatctgtact caagccttct
     4021 aaaagcttgc tggctacttc tacttgctcg ataacttttt cggctttccc ctgctccaag
     4081 gcttcgacca aagaaaccac cgtttctttt gaggaactta aaaaattttg attgatattt
     4141 tgcttgattt gctggaccat gtgactcgat acagccactt ccttggtcca tcccactaag
     4201 aaatcacatt ctaaagttgg tttcacttgt gagataaaaa atccccaatc acgctccaga
     4261 actgtcgcca agttttcttc ttctaaccaa gcagccacct tctggcgatc aaatgactgg
     4321 tagagaacca aatcctctgc cacaatacag gcaaggtcgc ccatggaacc attgtctcct
     4381 cgcttgagca agacagcgct agtcagcttg aacaagagct cctgatcaac agaaacatca
     4441 tacagagcca gtaaagcctt gacaaccaag acaacgacgc tgccactaga acctagacca
     4501 aactttttcc cttctcgttc cattttgcca cagatttcta gggaaaaagg tcttaaattc
     4561 tgaccgcgaa cagcgaagaa gtctcccatc aaagcaatcg tttcttgaat caagctgtag
     4621 tcaggattgg gccttaagtc cactgcgaaa tcaaacatat ctgaatagat acggtagctg
     4681 tcagaaaaag caatatcagc cctcatatag atgggaatat cctttatcaa agctaactgc
     4741 cctggctcta aaatagcata ttcacctgcc caatagagtt ttccgcaagt tttaacagca
     4801 atcatcttga ctcaaatcct ttgtttttga cacaatcaag cgataacgct gaccgaaaat
     4861 ttctgataaa tgctccaagt ctttctcctg acagaagacc ttaacattgg gaccagcatc
     4921 catggtaaag tagcaggcct ctcctttctc acgaagctgg cgaacaaagt ccatagcctc
     4981 ataagaggca tccgtcagat aagaaaaggc tggactagca gtctttgtcg tagcatgcat
     5041 agccagggca tttttctccg ttaattctcc aatcttggca aaatcatttt ccttgagata
     5101 aatcagcata tcctgatagt ccttctcaga ctgacgaacc cagtcgtcaa aagtcgtcga
     5161 ggtttccaca caaagtttca tcccatcacg gctagagatt ggttttttct tgtcctctag
     5221 caccaacata atcatagcta gtttcaagtc tgtctctaca gggtaaattt ctccactatc
     5281 cttatcccag gctcctagtg gtccataaaa actccgagaa gaagagcctg aggcaaactt
     5341 ggcttcctgc gctaactgac ttctatccaa tccaagcttg aaataagcat tacaagcctt
     5401 gaccagggcg gacaaaccac tagaacttga ggacaggccc gctgccgtag gcatattgtt
     5461 ttgagtatcg atacggacaa agccctcacc agctggacga taacggtcaa taatcttact
     5521 catcttggca tgctcgacct cattttgtag ctgaccattg atgtaaaatt cgtcagctgt
     5581 tacattggct ggtaaaggcg acaaggtcgt ctctgtatac atattttcca aagttagaga
     5641 aatactgcta gtagcaggca ccatctcttt ttcttttttc tttccccaat atttgataat
     5701 agcaatattt gcgtaggaac gtactgttac aggctctcta tccatgtctg aacagctcct
     5761 ttctcttcta atctttctgc tagttcttgt gcgtgtgtca aattggttac caaggctatg
     5821 atacaacctc ctagcccacc accgctcatc ttggcaccca gagcaccatg gctaagagtc
     5881 gtttcaacca aaaagtctgc ctcagggcta ctgactccaa tttcttttaa atgtaaatgc
     5941 gcttgactga ggatttgtcc cagtccttca gcatcttttt gtgaaattgc aacttctgct
     6001 tgctgggtta attctcccaa ggcatgcaaa aacggtaggg catccttgcc cttattttga
     6061 accacttgga tggcttcacg agtatgacca taaacacccg tatcggcaat caccaaatag
     6121 gcggataaat ccatctcaag ttctgtaaat cctacgttct tgataaagcg aataggttgg
     6181 tcactgagac aggtcttagc atccaaacca ctaggattca tatgggcaat catttcagct
     6241 cgattgacca agatttctag tacatcatga gacagatcag cctgatagta gtcaaatacc
     6301 gcacgaatgg ccgctatgct gatagccgct gacgaaccca tcccccgttt ctcagggata
     6361 gccgagtcaa tctcacaacg aatgcaggct tctgtgatat ccaaatactc cagtgaggca
     6421 taaaccgcca tggacaaggt atcctcctca taaaggcgcc aaggactctc tgcaggaact
     6481 accttacagg tcacctccac ctccaaaaga ggcagggaaa tggcaggata accgtaaacg
     6541 accgcatgtt cccctattaa aattatctta ctatgtgcct gaccgacacc aacttttttt
     6601 gtcatttttt ccttttacta gacgaaaaaa cgtcttattt ttcatacaag tattaattct
     6661 ttcctatcta ttttattata ttttcacaaa aaaagcgatt gtttccattc acaatcgctt
     6721 ctttcattac cgaacccatt caccattata gttgacagaa tagccatcta cagtcgtatt
     6781 cactgccaag gcacctgagc tataagcgta gtaccatctg ccattgacct ggaaccaacc
     6841 tgtcgtcata gaacgacgaa agaaactcca taccattaag taaagaggaa agtcgtgagg
     6901 gagcatgcgc cattgacaac ctgttttagt gacgtacaaa gtctcattaa caagtactcg
     6961 tttcggccat ttataggtgc ggtgtttgga gaaatagggt tcaatcttcg cccactcttg
     7021 atcgtttaaa tcagtatcat atgctttgcg tatcatatct ctagtttaac atttttttgt
     7081 gaatacaggt tctaaataat cgaccacgaa aatttcttaa gtggaaaacg ccttatgaag
     7141 tatgctacgg taaagttata cacttaattt gacaattcaa gatgtaaaaa ta
//
LOCUS       DAVMEI010000087         6850 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVMEI010000087 DAVMEI010000000
VERSION     DAVMEI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.087"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..3370
                     /locus_tag="VYQ24_001406"
     CDS             20..3370
                     /locus_tag="VYQ24_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1968535.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSLLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3641..3841
                     /locus_tag="VYQ24_001407"
     CDS             3641..3841
                     /locus_tag="VYQ24_001407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968536.1"
                     /translation="MKIKEQTRKLAASCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(3958..4428)
                     /locus_tag="VYQ24_001408"
     CDS             complement(3958..4428)
                     /locus_tag="VYQ24_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1968537.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4445..6718)
                     /locus_tag="VYQ24_001409"
     CDS             complement(4445..6718)
                     /locus_tag="VYQ24_001409"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1968538.1"
                     /translation="MRFNQYSYINFPKENILSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSENELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSSQDVIREVVYVESRVDTDQKGLPDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVSLPHKIELEKPQLNLVQPQGKAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFATLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNDRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         2099 a         1159 c         1487 g         2105 t
ORIGIN      
        1 tgtttaaagt gagaaaaaaa tgggaaattt tagctttctt ttaaaaaatg acgaatatga
       61 atctttttca aaaccttgca ttgaagctga gaatatgatt gctacatcaa ctgtggctac
      121 tgcctttatg gcgcgtcgtg ctttagagca ggctgtccat tggatatata gtcacgattc
      181 atatttagaa gctccctatc gtgctactct atcttcttta gtatgggatg atgattttag
      241 ggatatcgta gattctgaac tccacaagca gatagttctg ttgattcggt ggggaaacca
      301 tgctgctcat ggtggtgaaa ttaaggaacg agaagcgatt ttagctttgc atcatttgta
      361 tcagtttgtt aattttatcg attattgtta cagcaatgag tttgtggagc gttattttga
      421 tgagaagtgc ttaccacttt cagcaaacat caaataccga gaaactccac aatctatgat
      481 aaagttacaa gacagtttac cagaactgcc tgattttcat gaacagatgg ctgctcagtc
      541 cgtagaagtt caagagactt atactgaaaa acgtgagact gcagcgcaac ggcaagatgt
      601 gcctttccat attgatcaat tatctgaggc agagacaaga aagctcttta ttgatatcga
      661 tctccgttta gcaggatgga tatttgaaga aaactgtcgt gttgagatag ccgttgatgg
      721 tctcaagcac ggttcaggaa ttggttactg tgactatgta ctttatggta aaaatgggaa
      781 aattttagcg attgtagagg ctaaaaaagc ctctgtcaat ccagaagtag gggaagtaca
      841 ggtcaaagaa tatgctgaag ctcttgagaa acatatcggc tatcagccaa tttgctttat
      901 tacaaatggc ttgaagcact atatacttga tggtccgaac cgccgccaga ttgcaggctt
      961 ttactctcaa gaagaattgc aattagtgat ggatagacgt catcttcaaa aaccacttga
     1021 ggatatttct agtaaaatta gggacgatat ttccgggcgt cactaccaaa aacatgccat
     1081 tgcaagcgtt tgtgaagctt tctctaatca tcgtagacag gcacttttgg ttatggcaac
     1141 tggggctggg aaaactcgta cagcagtttc tctagttgat atcttatcac gtcataactg
     1201 ggtaaaaaac gttctcttct tagccgatag aacttccttg gttaagcaag cctatgattc
     1261 gtttagaaaa ttactcccag atctttccgt ttgtaacttc ttagaagata aagaaggagc
     1321 tcaatcaagt cgcatggtct tttcaactta tccgaccatg attggagcga ttagtggtca
     1381 agaagaagta aatcaacgcc ctttcactgt tgggcatttt gaccttatca taattgacga
     1441 atctcaccgt tctatttatc agaaatacaa gtccattttt gattattttg atgcaagaat
     1501 tgtaggctta acagctactc cgcgtcaaga tttagataaa aacacctatg gattctttaa
     1561 tttggagaat ggggttccaa catatgcata tgatttggaa gaggctgtta aagacggata
     1621 tttagtagcc tatcattcta tcgaaaccaa actgaaacta cctacggatg gtctacatta
     1681 tgatgatttg tccgaagaag aaaaggaaca ttttgatagc aaatttgaag acgatagctg
     1741 tgaaaaagat attgatggga gtgtatttaa ttcctttgtt ttcaataaaa gtacagtaga
     1801 aattgtttta aatgaactca tgacaagagg aattcagaca gcctcgggtg atgaaattgg
     1861 taaaactatt atttttgcta aaaatcatga tcatgcggaa tatatcagag gtatttttaa
     1921 caaccgctat cctgaaaaag ggagcgacta tgctcaggtg attgattata gtattaagca
     1981 ttatcagacc ttgattgatg attttaaaat taaggagaag tatcctcaaa ttgcgatttc
     2041 tgtcgatatg ttagatacag gtattgatgt accagaggtt gttaatttag tcttcttcaa
     2101 gaaagtacgc tctaaaacta agttttggca gatgattggt cgaggaaccc gtctatgtaa
     2161 agatttattt ggacctgagc aggataagga aaacttcttg gtatttgatt atggggacaa
     2221 ttttgattat tttcgtgcag atccaagaga tggagagggt cgtcacattg tttcgttgac
     2281 tcagcgttta tttaatatca aagtggactt gattcgagaa cttcagggac tccaatacca
     2341 agaagatcag tttgcgagag cataccgtca gcagcttgtc tcggaacttc aaggtcgtat
     2401 agagagctta aatgagttgg acttcagggt tcgtatggtt ttagatacag tttatagcta
     2461 taggaaattg gaaagttggc agaatctaac tgctgttaca agtgaaacca ttcaaaaaaa
     2521 tctctctctg cttttatttg atgaagataa agaagatgag atggcgagga gatttgattt
     2581 gtggttgctt catattcagt tggggcaact gacagctaaa tcttccactg ttcatatttc
     2641 ccaagtgatg aagacggcta gagctctttc tgctattggc aatatcccgc aggtttttga
     2701 gcaggctgaa attatcagga aagtacagga gcctgaattt tggaaagaag ttaacttgtc
     2761 tgatttggaa aaaattcgtc ttgctattcg agatttatta cagtttttgg ataaaacaga
     2821 ccgtaaaccc tactatgtta actttgaaga tcgtatactc tccactgttc acgagaccac
     2881 agcatttttg caggtcaacg atcttcggtc ttacaatgaa aaagttgagc attatttgaa
     2941 aactcatctg gatgaggagt ccatttctaa gctataccat aataaaaagt tgacatctga
     3001 tgatatgctt gcacttgaaa aattgctttg ggaaaaatta ggtagtaaag cagactacca
     3061 aagtcattat gaaaataagg caattccgag attggttcgt gagattattg gcttagatag
     3121 agagtctgcc aatcgtattt tttctaaatt tttgtcggat gagaatctta atgccaggca
     3181 gatttcattt gtaaaattga ttgtagacta cattgtagaa aatggttttt tagagacgaa
     3241 agtgttaacg caagagccgt ttaaatctta tggttctgtt caactactct tccaacacca
     3301 actaccagta cttcgtaata ttgttcaaat cattgaactt atcaataatc gagctggaga
     3361 agcggcttaa attctaaagt gattgccatg ctgagactca tttaaaatta aaaagagtag
     3421 aaatttatgc tatatatgag aagttttatt aggaagaatg tcatcgtttt cctagaatac
     3481 agtatcagtt gttaagtggt tgataaattt caaagtagat acttgtacca cgatgtttgt
     3541 tgatcgagtt attaacaaaa gagctacttt gattttaaag aaatagaaaa caaaaagccg
     3601 agcaagaatt caattgcagg agaaaatgaa ataatactca atgaaaatca aagagcaaac
     3661 taggaaacta gctgcaagct gctcaaaaca ctgttttgag gttgtagata agactgacga
     3721 agtcagctca aaacatggtt ttgaggttgt agatgaaact gacgaagtca gtaaccatac
     3781 ctacggcaag gcgacgttga cgcggattga agagattttc gaagagtata aatcttccta
     3841 ggataaagca aaacgcatag tatcaagggt tttcagcact tgatactatg cgttttctga
     3901 tgttaaagac tttctaccag gttttttaaa agcataattg ttagttgtag tcatttatta
     3961 ttcttcaaag aaaaatggtg gggcgaattt tttcagttct tcaaagcact tttgagcagt
     4021 atctgcatct tcacagatga taagacagac atcattacca caaagggtag cgatagcgtc
     4081 agggaagctc aaagtatcaa tgatagaacc aaaggattga gccagtccag gaagggtttt
     4141 tagtaggact tggtgttgaa ctgggcgcat ccagacaagg gcgtcttcca tgtagagttc
     4201 gagacgtttt tcccattttg agatggaacc attgttaaga acataataag cgctatcttc
     4261 ttcgcggact tttgataggt tcatattttt gatgtcgcgt gagagggttg cctgggttac
     4321 ttgaatgtcg ttctcagcaa gaagggcttg caactcagcc tgtgtatgaa tcttgttttt
     4381 tgtgataaga gcgcgtataa gttggtggcg gtgttctgat ttattcataa taatgtaact
     4441 cattttagca aggtaaggta agcatggact gagcgaggtc gacagtcaag tggtagtctg
     4501 tattgtcacg gatggtgatt tcaaagtcag tagtatagag gactaaacgg agagtgtctc
     4561 cttcttttag cttgtaaata gttggctgca gttcaaattg aacgtccatc cattcatctg
     4621 cagtaatatc ctctactaac agtaaatcat ttctattttg taaattaagg taaccttttg
     4681 tcacgactcg ttgtgcctct ggtctaaatg gcaattcaca gagattttcc aacatgtgat
     4741 agcgaccgtt atcaatggtt ctagcactta aaatagctgg ataaggttgt aggtatttct
     4801 tttgcccaaa ttctagcagt tgggcagata agagcccctt gtttgtactg gatttgatac
     4861 gaagattgag ctgagcgcga ccgtttaggt ggagatcttt agtcacagga aggttaatag
     4921 taatctgatt ggctttccct tgatagagct ctgtattgaa ggtttggtat gtcttaccat
     4981 agcgctcaaa atccttatct gggtactggt tttgaatagc ttgctcttct tgaccaagtg
     5041 agaaggtttc acagttttct tgcccaccga agttatcaag tgataaccaa gtctgtggag
     5101 ctgtattgtc ctgccagata acagtaggaa gttgaaagtc tgtttcctgt cctagtaatt
     5161 tcttggtcaa taaggcattt atggactcac ggaagtcaat tgattgccaa ttgttcatgt
     5221 aaacatgagc accattatgg aaaaagagat gcttgtgtat atgagtagga agagcatgga
     5281 acatctggta aacatgaagt ggtttgacat tccaatcctg agaaccatga gtaaagacaa
     5341 cctctgcctt tactttatgg gcattgagca gataattgcg gtcatgccaa aactgattgt
     5401 agtccccagt ttttcggtct agctgagctt tcactttttc taagtcagct tggtgagctt
     5461 cattgccacg gatatagtcg ccagctaaga gattacgaga ataggttaac tcagcaaggg
     5521 agtcaaagtc ctcacctgga taaccacctg ggctagtcac cagaccgttt tcacggtagt
     5581 agttgtacca tgatgaaatt cctgcctcgg caatgataac ttctaaacca tcgactcctg
     5641 tagtcgcaag accattggac atggtaccta gataggaaag tcccgttgta gcaacttttc
     5701 cgtttgacca atcagccttg acttgacgct ggcgcgtgtg atcagtaaag gcacggcaac
     5761 ggtcgttaag ccaatcgatg acatttttat aagcctcgat ttgctggtag tctccattag
     5821 tcatgaaacc tgtcgagtct ttggtaccaa cacctgagac atagagagtg gcaaagcctc
     5881 tcggaaggaa gtagtcgttt agtgtatagc tagagttgat gtgagttagc ttttcctcag
     5941 cctctgctac aagctcagct ttaccttggg gttggacgag atttagttga ggtttctcta
     6001 gctcaatctt gtgaggaaga gaaacctcaa gctcgccctc catcttgtag agagccttgt
     6061 cactagcctt atcattggtt ccctgatgat aagggctggc tgtcatgatg gcagggattt
     6121 ttccatcaaa acgagggcga ataatgctaa cctttactag gtctggtagc cctttttggt
     6181 cagtatcgac acgagactca acgtaaacga cttcacgaat gacatcctgg ctagaaaaag
     6241 tagccaaact cttgccgtta aagtagtggt agtcattatc ctccggaata agaccatcac
     6301 taacaagttg gtcgataaga gtatttcctt ttttggtgcg agtattgagt aactgataga
     6361 gattttcaat caagtcacca tatataatgg gaaatccagt ttctttacga aaaacgtcac
     6421 tatcttcgaa gtcaaccaaa taagaaaagc ctaaaagttg aaaagcaaca gtataaaaaa
     6481 tatctgctgt cagttcattt tccgattgaa aaaatgtcag caggtctgtt tttttatcag
     6541 ctgctaggat ggaaagtggg tagttggtgt cttgataagt gaaaaagaaa cgacgtaaaa
     6601 aggtttcaag tgagtctttg tgattggctg tattttgtaa atcaaagcca cattttttta
     6661 gttcagataa gatattttct tttggaaaat tgatataact atattgatta aaacgcatag
     6721 aacctccata tagaatgaca gttaaggtta ttatatcaaa aaaaaagcag aaagggaatt
     6781 gttaacttca aaaggaaata atccaataaa aatgaataaa gtactaaatt cagtatagag
     6841 aacagagtaa
//
LOCUS       DAVMEI010000088         6844 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVMEI010000088 DAVMEI010000000
VERSION     DAVMEI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.088"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            8..757
                     /locus_tag="VYQ24_001410"
     CDS             8..757
                     /locus_tag="VYQ24_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1968539.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSE"
     gene            827..1345
                     /gene="rimM"
                     /locus_tag="VYQ24_001411"
     CDS             827..1345
                     /gene="rimM"
                     /locus_tag="VYQ24_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1968540.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            1335..2054
                     /gene="trmD"
                     /locus_tag="VYQ24_001412"
     CDS             1335..2054
                     /gene="trmD"
                     /locus_tag="VYQ24_001412"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1968541.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            2066..2404
                     /locus_tag="VYQ24_001413"
     CDS             2066..2404
                     /locus_tag="VYQ24_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968542.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(2435..2755)
                     /locus_tag="VYQ24_001414"
     CDS             complement(2435..2755)
                     /locus_tag="VYQ24_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968543.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            2853..3068
                     /locus_tag="VYQ24_001415"
     CDS             2853..3068
                     /locus_tag="VYQ24_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1968544.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(3137..3217)
                     /locus_tag="VYQ24_001416"
                     /pseudo
     CDS             complement(3137..3217)
                     /locus_tag="VYQ24_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3273..3809)
                     /locus_tag="VYQ24_001417"
     CDS             complement(3273..3809)
                     /locus_tag="VYQ24_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1968545.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            4235..4573
                     /locus_tag="VYQ24_001418"
     CDS             4235..4573
                     /locus_tag="VYQ24_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968546.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            4639..6621
                     /locus_tag="VYQ24_001419"
     CDS             4639..6621
                     /locus_tag="VYQ24_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968547.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIQGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYSIVF
                     ILIYQYVQLFITLKQNEYVKKIILGLSKTYIAISSLKYFMMTITMVILFTFLMTGQIE
                     LLYIGAASLLVLMLSIIMSFRKLSESYTKILKGDES"
     gene            6618..>6844
                     /locus_tag="VYQ24_001420"
     CDS             6618..>6844
                     /locus_tag="VYQ24_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1968548.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFF"
BASE COUNT         2339 a         1058 c         1351 g         2096 t
ORIGIN      
        1 tttctttatg aagaggtttg aagtatctac agaaattggt agcctctctg ttacttatca
       61 aaagcaaaag aaagtgctag tttgtctaaa cggagcaggt ttgctaccaa gttatgaaaa
      121 tttttcactt atacttgaaa aacctcctcc tacaattggt tatttgacaa ttgattttcc
      181 gaacacaggt aggagtccga ttcatgacca agctggaaaa aatctagata atcttgcaga
      241 tgcggtttat gaagtacttg aagaattagg aatttctgaa tatatacttt gtgcacatag
      301 ttggagtgga attttagctt gcaaattact cgagaaacca attaagcgtc agactttagt
      361 agcaattgaa ccgacaacta aaaaagtcat gtttgctgat ttttcagaaa atccttatcc
      421 agaaatggaa gagcagatga ggctgattga cgagtgtggt cctgaacttt attttaagaa
      481 cttaactcaa gcaacattta gtcctgaaac gaataaaaaa atctgggaat taatgcaaga
      541 aaaaggctta gagttggaaa atcaagatcc agaatttcag atatctggag agattactga
      601 ggaagatttt gagaatttgt cgatagaatc tcatgtccct gtatttattt tttgtcagac
      661 ttatagagaa aaagagtaca gagaatcaga atattggact tccaatacta aactcatttt
      721 aggaaggaat caccattatt tacaatggtc agaataggaa aaaattgcgg ctattattcg
      781 agaattgtca gaataagatg gaaaaaagga gattacagga gacaagatga actactttaa
      841 tgttgggaaa atcgttaata cgcagggatt acagggtgag atgcgagtct tgtctgtgac
      901 ggattttgca gaagaacggt ttaaaaaagg agctgagctg gctttgtttg atgaaaaaga
      961 tcagtttgtc caaacagtga ccatcgctag ccaccgtaaa cagaagaact ttgacattat
     1021 taaattcaaa gatatgtacc atatcaatac tatcgaaaag tacaagggat acagtctcaa
     1081 ggtcgctgag gaagatttga atgacctaga cgatggtgaa ttttactatc acgagattat
     1141 cggtttggaa gtctatgagg gtgatagctt ggttggaacc atcaaggaaa tcctgcaacc
     1201 aggtgctaat gatgtctggg tggtcaaacg aaaaggcaaa cgtgatttgc ttttacctta
     1261 tatcccacca gtggttctca atgttgatat tccaaataaa cgggtcgatg tggaaatctt
     1321 agaagggtta gacgatgaag attgatattt taaccctctt tccagagatg ttttctccac
     1381 tggagcactc aatcgttgga aaggctcgag aaaaagggct cttggatatc cagtatcata
     1441 attttcgaga aaatgctgaa aaggcccgtc atgtagatga tgagccctac ggaggcggtc
     1501 agggcatgtt gctcagagca caacctattt tcgattcctt tgatgctatt gaaaagaaaa
     1561 atccgcgcgt tattctcctc gatcctgctg gaaagcagtt tgatcaggct tatgctgaag
     1621 atttggctca agaggaagag ctaatcttta tctgtgggca ctatgagggt tatgatgagc
     1681 gcattaagac cttggtaaca gatgagattt ccctaggcga ctatgtcctc actggtggag
     1741 aattggcagc tatgaccatg attgatgcta cagttcgcct gattccagaa gtgattggca
     1801 aggagtctag ccaccaagat gatagttttt cttcaggtct tttagaatat cctcagtaca
     1861 cacgtcccta tgattatcga ggcatggtcg tgccagatgt attgatgagt ggccaccatg
     1921 aaaagattcg tcagtggcga ttgtacgaga gtttaaagaa aacctacgag cgcagaccgg
     1981 atttacttga acattatcaa ctgacagtag aagaagaaaa aatgctggca gaaatcaaag
     2041 aaaacaaaga ataaaggaga aacctatgca agtaatcaaa cgtaatggcg aaattgctga
     2101 atttaatcca gataagattt accaagccat cttgaaggca gcccaaactg tttatgtttt
     2161 gacagatgat ttgcgtcaaa atcttgctca agtcactaag aaggtagttt tggatttaca
     2221 agaagccaag gtggaacgtg cgactatcag tatgattcaa tctatggttg aacatcgttt
     2281 attgggcgca ggttacatta ccattgcaga acactacatt tcctatcgtt tacaacgtga
     2341 cttggaaaga agtggttatg gagatcatat tgcagttcat ttacattttg aacaaattcg
     2401 ctaagaaaaa agagtgggat gaagcaacta catctcactc tttcttaaat ctattttttt
     2461 gggctgtttg gacagttgag gggacaaatc gcaggcgttg aatatcgctt atacggataa
     2521 tccggctcac atttttggca aaatttttca cgataatttg ctggccttgc tgatcatatt
     2581 tgatgatatc acctgtaaaa cttgtctcag acaagataag gtgaacagcg gtttgtttct
     2641 ggatagcctc ttcaatagtg gctgtaaggg agttggtttc agtctggtca ggttggtcat
     2701 gtccatttaa aaaatcatgt attttatcta gaacttgctg gaatttatgt ctcatattgg
     2761 cctcccttct tttatatcaa tattatataa aattttccta aaatctacaa gattttacga
     2821 ataaactaat gaaactaaaa aaggagaaga aaatggcaga atttacattt gaaatcgaag
     2881 agcacttgtt gactctttct gaaaacgaaa aaggttggac caaggaaatt aaccgtgtga
     2941 gctttaatgg tgcccctgca aagtttgata ttcgtgcttg gagtccagac catactaaaa
     3001 tgggcaaagg gattactctc tcaaatgaag aatttcaaac gatggtggat gcctttaaag
     3061 gcaactaata ctcttcgaaa atctcttcaa accgcatcag ctttgccttg ccgtatgtat
     3121 ggttactgac ttcgtcagtt tcatccacaa cctcaaaaca gtgttttgag cagcctgcgg
     3181 ctagcttcct agtttgctct ttgattttca ttgagtataa gttttcaaaa taaaaaaaag
     3241 gatattgagt catgataact cggtatcctt ttttagttag taaagtaagc ctgattttta
     3301 aggcgttgaa gtaggggacg gctaataaaa ctaatagcca gaagtttgcc aaggtctgga
     3361 atgataaagg gaagaacccc cacaacaaga gctttttcaa atgccattcc agctaggaaa
     3421 tgcaagctga gaatcccgcc gacaaagaca agggcatcac ccaagaggtt tgcaagaaaa
     3481 atcttaacaa caccactctt gctgttggtt agagaggaag taagtccaga gtaaacgaga
     3541 taaaaccaaa gatagcctgc agtagggcca actaaagcct gaaaaccagc tccacctcct
     3601 gcaaagacag gaagaccgat agcacctaga agaagataga gtccagcaga aagtacagcc
     3661 tctctcggtc taaagacagt agcaatcaag ccgattgcaa agttttgcag agtgaaggga
     3721 acaggtccaa ttggaagact gatttgtgcc aaaacagcaa tgagagcagc cccaatagca
     3781 gggatagcat aaacgtgagc ttttttcaaa atagttaacc tcttttctaa tgtatagtaa
     3841 ctattatact catataggag tcgatgtcaa ccatttttta aaaacaaggt aacaaaaact
     3901 gtaacagccg attttcgtga ctagtcaagt tggataattt ttttgttgat tcgaaaattg
     3961 tctgtataga taatcctatt ttattaataa aaaatatact agtaaatact tgacaaatta
     4021 aaaaataagt gttacattaa aaatgtaaac ggatacattt agatgtgttc aattttaaaa
     4081 ggaggggtag ttatgagata ttttaacgac tctagatacc ataacggtga ttaaaaacct
     4141 aaatataatc aattcatatt aaagtaatat ggagggacta aaaagagact attatttaat
     4201 cttcatcaaa tatttaaatt caaggagaag taaaatgaaa aaaagaatta ataagaaagc
     4261 atctgttttt ctagttgcaa gtgttttcag ttttagcttt gctactacag ctagtgtcgt
     4321 taacgcagta acggcacacc ctgctccaag tcccattatt gatgtatggg aatatggtgt
     4381 aaatgataac tatggttatt cgaattactt tgttagttct ccaagtaata ttggatcaaa
     4441 atcttcagta acgaattttt ggggaacagt taaagctcag gataaaaaag attatggatg
     4501 ggcatattca agtgcaacaa aatcttggaa cgatgttcgc ttaaatgcat attgggatta
     4561 ttatagattt taaaaataat ttatgaaaac aagagaccca attataagat tgggtctctt
     4621 gtaatatggt aattgcttat gaaattaaaa ttatgtatca ttggattctt cttttgtttg
     4681 attgctacaa ttggtctggt cactattagt gataccgaaa tccccatacc tttaccaatt
     4741 gatggagctt tttctatcca aggaaaaagt aatctttcta ataacgaaat atacgaaatg
     4801 gttcgtgact tatcaaaaac ggaaaaggtt accatttaca agccaatcgt tcagagttcg
     4861 ggtcagttga agtatgtaaa ttttgatgat gtaaataatg aacaattaaa gtcagcacca
     4921 atagtaggga tgtattacac acttgggaaa atggatgtag acagtttgaa accacttacg
     4981 atgacgggac tacaaacagt ctatatggct tatccttggt atataggagg aatattacaa
     5041 ttcactggaa ctttaagaat actgttaatg ggttcaattt atttaacttt attagttgtt
     5101 ttatttgttg ttaggacgag gcagatcaaa gaaggagtga tacgacgttc tttgggcttg
     5161 cctatatacg acttaagaag agagtatggc atttctctta tttttgaact gattatgatg
     5221 gctttattga tgatttctta cagttctttt ttgggtaatg gtttcttcac ctatagttct
     5281 aagttatttt tctctctgct tttaacgaat tttatactat ttcaaatcat tgatcttatt
     5341 acttttgttt tattttggtt gacgattcag attgaaaagc ctattgaaat tattaaaaac
     5401 aaggcaaaaa acaagcttat ttttgtcgta tggcttgcta ttatttcgat tatcatcctt
     5461 gtctcaggag tttttttaca agaaacaaag agtagtcaat ctagtattaa catacagata
     5521 cagaatttag taccatggga tacagtaaaa gactggagaa ggattgagtt ccttggaatt
     5581 gaaagtaact ctactaaaaa tagagaagtt aatgattcag atggtcagta tctacagata
     5641 gttgctgcct taaaaaactt agatttctta tatatagaac ggtcttcagc atatgttcca
     5701 gatttcatga aaacttcaca tgttatagaa aatttttcta aacaattaga aaatgacggg
     5761 ataacgaatc cagaaataaa taaagagtta atttatatca accaaacagg tgctaaccta
     5821 caaaataaag tgaatggaac aaactatcat cttttagata ataagatagc aaccatctat
     5881 attcctgaga agtggaaaga aaaccagaaa tctattgaga atacagttgt agcagaacaa
     5941 tttattggaa caaactatac aagagaacag cttgcagttc aaataattcc tgatggggaa
     6001 aaaatttttt attttaatga ggatgctgat aataatctta aaatgaaaga tattttaccg
     6061 ctggcaaatg tagcagatag caaagataat atagttgttg tgttagatac ggacaaaatg
     6121 atggaaaata ataagttttc tttggctagt aacatactat ataaatctct ttttagtcct
     6181 gaagcggtaa aaaaaataaa tgagatgact gtcctgttga atttctcaat gaatccagta
     6241 gatgtttacc aaattgtgaa attaaagatt caatccttag agcaccaaat tttactttca
     6301 caaatcttgc agaagataat ttacagtatt gtctttatac tcatttatca atatgtccaa
     6361 ctttttatta ctctaaaaca gaatgaatat gtgaaaaaaa tcattttggg tctgtcaaaa
     6421 acatatatag caatttcaag tctcaaatat tttatgatga ctattacaat ggttattcta
     6481 tttacatttc ttatgacagg gcaaatagag ttgctatata ttggagcggc ttctctgcta
     6541 gttttgatgt tgtcaatcat aatgagtttt agaaaattat ctgaaagcta cactaaaatt
     6601 ttaaaaggag atgaatcatg acaattgacc ttttgaacgt ttcaaaaagt ttcggctcaa
     6661 agaagatctt tacagactta aatttaatat ttgaatctgg aaaaagctat gcattgattg
     6721 gaggttctgg ctccggtaaa agtacccttc ttaatattat aggaagatta gaaaaaattg
     6781 acagtgggaa tgttttagtt gataaacaag atatttggaa gataaaagaa agaacttttt
     6841 ttaa
//
LOCUS       DAVMEI010000089         6757 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVMEI010000089 DAVMEI010000000
VERSION     DAVMEI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.089"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(76..339)
                     /locus_tag="VYQ24_001421"
                     /pseudo
     CDS             complement(76..339)
                     /locus_tag="VYQ24_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            537..2303
                     /gene="patB"
                     /locus_tag="VYQ24_001422"
     CDS             537..2303
                     /gene="patB"
                     /locus_tag="VYQ24_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1968549.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     NQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2442..3131
                     /locus_tag="VYQ24_001423"
     CDS             2442..3131
                     /locus_tag="VYQ24_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1968550.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3135..3314
                     /locus_tag="VYQ24_001424"
     CDS             3135..3314
                     /locus_tag="VYQ24_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968551.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3301..4650
                     /locus_tag="VYQ24_001425"
     CDS             3301..4650
                     /locus_tag="VYQ24_001425"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1968552.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAVVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4774..6234
                     /locus_tag="VYQ24_001426"
     CDS             4774..6234
                     /locus_tag="VYQ24_001426"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1968553.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            6248..6679
                     /locus_tag="VYQ24_001427"
     CDS             6248..6679
                     /locus_tag="VYQ24_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1968554.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERATLFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNE"
BASE COUNT         2122 a         1295 c         1453 g         1887 t
ORIGIN      
        1 ttctaactta gtcttaagat aggccttaaa agcaggtgaa aagcgagggc atgctttggc
       61 agcttgtatg agtacctacc gcagatgagg ggaaccccgt ttgaccattc ttcctgctaa
      121 atcaatctga cctgactgat aaatagaaga atccagtcca gcgaaagctt gtaattgagc
      181 aggattatca aaggcatgaa tatttcgaat ctcggctaaa atgaccgccc ctaaacgatt
      241 ctcaatccca gtaaccgtcg tgatgaccga gttgaactca gccatcaagt cattgacaca
      301 ttttttcgcc ttgtcaatga gcctcttgta atgtttgatg ttttcattac aagagataaa
      361 acgtttatgc gttatcaaac tcattaccaa ttgaaacaaa aagctgtggt tagatccttt
      421 cggaaattgt caagcgattg gaggaaatga actaatccac agcggcttat tccaagtata
      481 ccacttgggc tttggcagta gctaactgcg ctaaatataa tataaggagg agtaaaatga
      541 agacagttca atttttttgg cattatttta aggtctacaa gttctcattt gtagttgtca
      601 tcctgatgat tgttctggcg acttttgccc aagccctctt tccagtcttt tctggacaag
      661 cggtgacgca gctagccaat ttagttcaag cttatcaaaa tggcaatcca gaacttgtat
      721 ggcaaagcct atcaggaatc atggtcaatc ttggcctgct ggttttggtt ctatttatct
      781 ctagtgtaat atacatgtgt ctcatgacgc gcgtgattgc agaatcgacc aacgagatgc
      841 gcaaaggcct ctttggtaag cttgctcagt tgacggtttc tttctttgac cgtcgacaag
      901 atggcgatat cctgtctcat tttaccagtg atttggataa tatcctccaa gcctttaacg
      961 aaagcttgat tcaggtcatg agcaatattg ttttatacat tggtctgatt cttgtcatgt
     1021 tttcgagaaa tgtgacgctg gctctcatca ccattgccag caccccattg gctttcctta
     1081 tgctgatttt catcgtgaaa atggcacgta aatacaccaa cctccagcag aaagaggtag
     1141 ggaagctcaa cgcctatatg gatgagagca tctcaggcca aaaagccgtg attgtgcaag
     1201 gaattcaaga ggatatgatg gcaggatttc ttgaacaaaa tgagcgcgtg cgcaaggcaa
     1261 cctttaaagg aagaatgttc tcaggaattc ttttccctgt catgaatggg atgagcctga
     1321 ttaatacagc catcgtcatc tttgctggtt cggctgtact tttgaatgat aagtctattg
     1381 aaacaagtac agccctaggt ttgattgtta tgtttgcaca attttcacag cagtactacc
     1441 agcctattat ccaagttgca gcgagttggg gaagccttca gttggccttt actggagctg
     1501 aacgaattca ggaaatgttt gatgcagagg aggaaatccg acctgaaaag gctccaacct
     1561 tcactaagtt gcaagaaagt gttgaaatca gtcatatcga tttttcatac ttgcctgata
     1621 aacctatttt gaaagatgtc agcatttctg cccctaaagg ccagatgaca gcagttgttg
     1681 ggcctacagg ttcaggaaaa acgactatta tgaacctcat caatcgcttt tatgatgttg
     1741 atgctggtgg tatttatttt gatggtaaag acattcgtgg ctatgactta gatagtctta
     1801 gaagcaaggt gggaattgta ttgcaagatt cggtcttgtt tagcggaacg attcgagaca
     1861 atatccgatt tggtgtgcca gatgctaatc aggaaatggt tgaggtagca gcaaaagcaa
     1921 cccacattca cgactatatc gaaagtttgc ctgataagta cgatactctt attgatgatg
     1981 accagagcat cttttcaaca gggcagaagc aattgatttc aatcgctcga accctgatga
     2041 cagatccaga agttctcatt ctcgatgaag caacttcaaa cgtagatacg gtgacggaaa
     2101 gcaagattca gcatgccatg gaggtggttg tagcaggtag aactagtttc gtcattgccc
     2161 accgcttgaa aaccattctc aatgcagatc agattattgt ccttaaagat ggagaagtca
     2221 ttgaacgtgg taaccaccat gaacttttga agctaggtgg cttttattca gaactctatc
     2281 acaatcaatt tgttttcgaa taagaaagaa gttgtcctat gtgggcagct ttttcttgtc
     2341 cataaaaaat gtttatcaca gccttaaaaa aaacatatta gacgaaagtc attttgagtg
     2401 atatgatagg actatcgtta gtattcgaaa ggagaggcat catggctaga acggttgtag
     2461 gagttgctgc aaatctatgt cccgtagacg cagaaggcaa aatcattcat tcatctgtat
     2521 cttgtagatt cgcagagatc attcgtcaag tcggtggtct ccctttagtc attcctgttg
     2581 gtgatgagtc agttgtacgt gattatgtgg aaatgattga caaactcatt ttgacaggag
     2641 gccaaaatgt tcatcctcag ttttatggag agaaaaagac cgtcgaaagc gatgattaca
     2701 atctggtccg tgacgaattt gaattggcac tcttgaagga agcgcttcgt cagaataaac
     2761 caattatggc aatctgtcgc ggtgtccaac ttgtcaatgt tgcctttggt ggaaccctca
     2821 atcaagaaat cgaaggtcac tggcaaggac tacctttcgg gacatctcac tctattgaga
     2881 cagtagaagg aagcgtggtg gctaagctat ttgggaaaga aagtcaggtc aactcagtcc
     2941 atcgtcaaag cattaaagat ttggcaccta atttccgtgt aactgctatt gattcaagag
     3001 accaaactat tgaagcgatt gagtctatcg acgagcaccg cattatcggt ttacagtggc
     3061 atccagagtt tctggttaat gaagaagatg gcaatttaga attatttgag tatttattga
     3121 atgaactgta acgattggaa catctggtcg ttctttcttt tattttttca aaatttttag
     3181 aatgtggcat tttacgcaaa cgtttgaatt ctgataaaaa ttggagaaat tcgacaaaaa
     3241 aacttgaaaa aaacgaaggt aagcgttatg atagaaaaga agaaatattg gaggaagaac
     3301 atgtcacata ttaaatttga ttattcaaaa gttttagaca aatttgttgc accacatgaa
     3361 gtggaataca tgcaatcaca agtaacagca gcagatgaat tgatccgtaa aggaactggt
     3421 gctggtagcg acttcttggg atggttggac cttcctgaaa aatacgaccg cgaagaattt
     3481 gaccgcatct tgaaagctgc tgagcaaatc aagtcagaca gcgatgtttt ggttgtgatc
     3541 ggtattggtg gatcttacct tggtgcaaaa gcagcaatcg acttcttgaa tcaccatttt
     3601 gctaacttgc aaacaaaaga agaacgcaaa gctccacaaa tcctttacgc aggaaactca
     3661 atctcatcta cttaccttgc tgacttggta gagtatgttg cagacaaaga cttctcagta
     3721 aacgtaattt ctaaatcagg tacaacaact gaaccagcga ttgctttccg tgtctttaaa
     3781 gaactcttgg ttaagaaata cggtcaagaa gaagctaaca aacgtatcta tgcaacaact
     3841 gaccgccaaa agggtgctgt taaggttgaa gcagacgcta acggttgggg aacatttgtt
     3901 gttccagatg atatcggtgg acgcttctca gtattgacag ccgttggttt gcttccaatc
     3961 gcagcatcag gagctgacat caaagctctt atggaaggtg cgaatgcagc tcgcaaagac
     4021 tacacttcag acaaaatctc tgaaaacgaa gcttaccaat acgcagtcgt tcgtaacatc
     4081 ctttatcgta aaggctatgc aactgagatc ttggtaaact atgagccatc acttcaatac
     4141 ttctcagaat ggtggaaaca attggctggt gaatcagaag gaaaagacca aaaaggtatc
     4201 tacccaactt cagccaactt ctcaactgac ttgcactcac ttggtcaatt tatccaagaa
     4261 ggaactcgta tcatgtttga aacagttgtc cgtgttgaca aacctcgtaa aaacgtgctt
     4321 attcctactt tggaagaaga ccttgacgga cttggttacc ttcaaggaaa agacgttgac
     4381 tttgtaaaca aaaaagcaac tgacggtgtt cttcttgccc acacagatgg tgatgtacca
     4441 aacatgtatg tgactcttcc agagcaagac gctttcactc ttggttacac tatctacttc
     4501 ttcgaattgg caattgccct ttcaggttac ttgaatgcta tcaacccatt tgaccaacca
     4561 ggtgttgaag cttataaacg taacatgttt gcccttcttg gaaaaccagg atttgaagaa
     4621 ttgagcaaag aacttaacgc acgtctataa tagaagaaaa gagtggtttg cccactcttt
     4681 ttactctctt tatccataga aattggactc agccaagact tgtgatataa tatagaaagc
     4741 aaaaaggcag acgcctagat aataggagaa actatgtcaa aagatatccg cgtacgttac
     4801 gcaccaagtc caacaggact actacacatc ggaaatgccc gtacagcatt gtttaactac
     4861 ttgtatgcgc gccatcatgg tggaacattt ctcatccgta tcgaagatac tgaccgtaaa
     4921 cgccatgtcg aggatggtga acgttcacaa cttgaaaacc ttcgctggtt aggcatggat
     4981 tgggatgaaa gtccagaatc acatgagaat tatcgccagt ctgagcgttt ggacttgtat
     5041 caaaaataca ttgaccaact attagctgaa ggaaaagcct ataaatctta cgttacagaa
     5101 gaagagttgg cagctgaacg tgaacgtcaa gaagtagctg gcgaaacacc acgctacatc
     5161 aatgaatacc ttggtatgag tgaagaagaa aaagcggctt atatcgcaga acgtgaagca
     5221 gctggtatca taccaacagt tcgtttggct gtcaatgagt caggtatcta caagtggcat
     5281 gatatggtca aaggtgatat cgaatttgaa ggtggcaata tcggcggtga ctgggttatc
     5341 caaaagaaag acggttaccc aacttacaac tttgccgttg ttatcgatga ccacgatatg
     5401 caaatctctc atgttatccg tggagatgac catattgcta atacaccaaa acagcttatg
     5461 gtctatgaag ctcttggttg ggaagctcca gagtttggtc acatgacctt gattatcaac
     5521 tctgagactg gtaagaagtt atctaaacgt gatactaata cacttcaatt tatcgaagat
     5581 taccgaaaga aaggttacct tccagaagca gtctttaact ttattgctct tcttggttgg
     5641 aacccaggtg gtgaagatga gattttctct cgtgaagaac tcattaaact ttttgatgaa
     5701 aaccgcctca gcaagtcacc agcagccttt gatcagaaaa aattagactg gatgagcaat
     5761 gattatatca agaatgcaga ccttgaaacc atctttgaaa tggcaaaacc attcttagag
     5821 gaagcaggcc gtttgactga caaggctgaa aaattagttg agctctataa accacaaatg
     5881 aaatcagtag atgagattat cccattgaca gatcttttct tctcagattt cccagaattg
     5941 acagaagcag agcgcgaagt catgacgggt gaaacagttc caacagttct tgaagcattc
     6001 aaagcaaaac ttgaagcgat gacagatgat gaatttgtga cagaaaatat cttcccacaa
     6061 attaaagcag tccaaaaaga aacaggtatt aaagggaaaa atcttttcat gcctattcgt
     6121 atcgcagttt caggcgaaat gcatgggcca gaattaccag atacaatttt cttgcttgga
     6181 cgtgaaaaat caattcagca tatcgaaaac attctaaaag aaatctctaa ataaagagga
     6241 tacaataatg gacatctggg aaaagatgta cgaagaagca cagaaactat acaatccaca
     6301 tgaagtatca gactttgttt atgccaatca tgttgtagct gcagtagaag caggagatgg
     6361 acaaatattt acaggattct gtatggaggg aacctgtggt gttttccatc tctgcgcaga
     6421 acgggcgacc ctcttcaata tgtaccaatt ttcaggacaa actaaggtta agaaagtatt
     6481 agcctttcga gacaaaccac cttatggtgg aagttcagcc atgccttgcg gtgcttgtag
     6541 agaattcctt ttagagctta atgctgaaaa taaagatgca gaattcatga tggactataa
     6601 tataagaaaa acagttaaag tcgcagaact aatcccttat tggtggggag aagaacgtgc
     6661 ttctaagttt aatgagtgat agaagagtca gtataattct ggctcttttt actataagtt
     6721 gaaaaaataa aaaaaattag aaaaagtagt tgacaaa
//
LOCUS       DAVMEI010000090         6717 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVMEI010000090 DAVMEI010000000
VERSION     DAVMEI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.090"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            40..849
                     /locus_tag="VYQ24_001428"
     CDS             40..849
                     /locus_tag="VYQ24_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1968555.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            854..1555
                     /locus_tag="VYQ24_001429"
     CDS             854..1555
                     /locus_tag="VYQ24_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1968556.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1580..2497
                     /locus_tag="VYQ24_001430"
     CDS             1580..2497
                     /locus_tag="VYQ24_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV1968557.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2525..4066
                     /locus_tag="VYQ24_001431"
     CDS             2525..4066
                     /locus_tag="VYQ24_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV1968558.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4085..4543
                     /locus_tag="VYQ24_001432"
     CDS             4085..4543
                     /locus_tag="VYQ24_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1968559.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4545..>6717
                     /locus_tag="VYQ24_001433"
     CDS             4545..>6717
                     /locus_tag="VYQ24_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1968560.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMK"
BASE COUNT         2245 a         1029 c         1310 g         2133 t
ORIGIN      
        1 ataaagaatg taaacgttta cttcaactaa ggagctcata tgttactgca aaaagaacta
       61 attccaatga tagaagctaa cttaccaaat atggcatatg ctgaaaaaga cattgctaaa
      121 ttcttcttaa aacagcaacc tctgaatgat tattcatcta aagcattgtg cgaatacctt
      181 aatgtatcca aagcaacatt gactcgattt gcgaaaaaat gtggttttaa aggttttaga
      241 caattcattt tcaaatacca agagatgatt catgagaaag aaaagttggc attatataca
      301 gaggcaacag aaaaagtttt atccgactat gaggaaatgt tgagaaaaac ttacacggtt
      361 cttgatgaag ttcaacttga gcgtattgct gagatgatag aaactgctga gcgtgtatat
      421 ctctacggta aaggaagttc tgttcttgct ttacaagaaa tgaagatgag atttatgcgt
      481 ctcggagtga ttggtgaagt attatcagac gaggatatga ttttgtggag tagcttacta
      541 cttaatgaaa attgccttgt cattggagca tccatttcag gtcaaactga tattgtacta
      601 gaaggtctac aaaaagctgc agataaaggc gctaaaacag ttttaatgac tacaagaaaa
      661 tttgacgaag aagattgttt ctttgatgaa ctattgttat tagcttcgac cgatcatctc
      721 tcgtatggca atcgcatatc acctcagttt ccaatacttt taattacaga ctgcttattc
      781 tctaattatc tggaaagtcc agagagacaa tattattaca atcaaactat tatccataag
      841 gaggaataat aaaatgagcc aaatttggac taaagaaaaa tttataagcc aagttcaagg
      901 tggagtcatt gtttcttgtc aagctttacc tggtgaagcc ctttataatg aagaatttag
      961 cttgatgcct tttatggcta aagcagcttt agaggcagga gcagtgggca ttcgcgcaaa
     1021 ttctgtgcgt gatattaaag caattcagaa agtagtagat ttaccaataa ttggaattat
     1081 caaaagggat tatccacctc aagaaccata tattactgct acgatgaaag aagtagatga
     1141 acttgtagaa tgcggaacaa cagtcattgc atttgatgca actttaagac caagatatga
     1201 tggcttagtt gtcagtgaat ttatcaaaaa aataaaagaa aaatatccga atcaattgct
     1261 gatggcggat gtaagtaatt tagatgaagg tctctatgca tttaaatcag gcgttgattt
     1321 tgttggtaca acattatcag gttacacaag tacaagtgta caatcagatg agcctgattt
     1381 tgaactaatg aaaaaattgg ctgattttaa tattccggta attgccgaag gaaaaattca
     1441 ttatccagaa caattaaaaa aagcttatag tttaggtgtt accagtgtag tcattggtgg
     1501 agcgattaca cgtccaaaag aaattgctca gcgatttatt aatgtcatca aataaaaaac
     1561 aatataaagg agaaatctga tgaaagattt aactaaatac aaaggcgtta tccctgcatt
     1621 ttatgcttgc tatgatgaaa atggtgaaat tagccaagat cgtgtaaaat ctctggtaca
     1681 atatttcatt gacaaaggtg taaaaggtat ctatgtaaat ggttcttcag gtgaatgtat
     1741 ttaccaaagt gtagaagatc gtaaacaaat tattgaagct gttatggaag ttgctaaagg
     1801 taaattaaca gttatcaacc atattgcatg taataacacg aaagatagta tcgaattggc
     1861 aaaacattca gaaagtgttg gagtcgatgc tattgcagct atcccaccta tttatttcaa
     1921 attgccagag tattcaatcg cagcatattg gaatgcaatg agtgaagctg cgtcaaatac
     1981 agattttatt atctataata ttccacaatt ggcaggggtt gcgttgactg gtagtttgta
     2041 tgcaacaatg cgtcaaaatc ctcgtgtgat tggagttaaa aattcttcta tgcctgtaca
     2101 agatattcaa atgtttgtag ctgcaggtgg agaagattac attgtattca atggtccaga
     2161 tgaacaatat cttggtggtc gcttgatggg agcagaagct ggtattggtg gtacttatgg
     2221 cgttatgcca gatttgttct tgaaattgga aagtttgatt caagaacgag atttagatac
     2281 agctaaaaaa cttcaatatg ctatcaatga agttatctat aagatgatat caggtaaggc
     2341 aaatatgtat gctgtagcaa aagaagtttt gcgtctaaat gaaaaacttg atttaggttc
     2401 tgttcgtcaa cctttagaag cattggcaga aggtgacttg gaagttgcaa aacaagcagc
     2461 agaacttatt caacaagcac gaaaagaatt tttataataa ataaaatcaa tatggagata
     2521 tgttatgggc acaacaggat ttacaataat tgacttaatt atcttgattg tttatttact
     2581 tgcggtgttg gttgcaggta tctatttctc taaaaaagag atgaaaggaa aagagttctt
     2641 taaaggagat ggttcggttc cttggtatgt tacttcggta tccatttttg ccacaatgct
     2701 cagtccgatt tccttcttgg gactcgctgg tagctcttat gcaggtagct ggattttatg
     2761 gtttgctcaa ttagggatgg tagtagctat tccactgaca attcgtttta tcttacctat
     2821 ctttgcacgg atagacatcg atacggcata tgattacttg gataaacgtt ttaattctaa
     2881 agcacttcgt attatttcag cactcttgtt tattatttat caattgggac gtatgtctat
     2941 cattatgtac ctcccatcag ctggtttatc agtattgaca ggaattgaca tcaatatttt
     3001 gattattttg atgggtgtag ttgcaattgt ttattcttat actggtggtc taaaatccgt
     3061 attatggaca gactttattc aaggtgtgat tctgattagt ggtgtcgttt tagctttatt
     3121 tgtactgatt gctaatatta aaggtggctt tggtgcagta gcagaaacat tagcaaacgg
     3181 gaaattcctt gctgcaaatg aaaaactttt cgatcctaac ttgctttcaa actccatctt
     3241 tttaattgtg atgggttcag gctttacaat cttgtcttcc tatgcttcat ctcaagattt
     3301 ggttcaacgt tttactacaa cacaaaatat taagaaactt aataagatgt tgttcacaaa
     3361 cggtgttttg tcacttgcaa ctgcaacagt cttttacttg attggtacag gcttgtacgt
     3421 attctatcaa gtacaaaatg cagatagtgc agctagcaat atccctcaag accaaatctt
     3481 tatgtacttt attgcatacc agttaccagt aggtatcaca ggtttgatct tggcagcgat
     3541 ttatgcagca tctcaatcaa ctatttcaac aggtttgaac tctgttgcaa cttcatggac
     3601 attggatatt caagatgtca tttctaaaaa tatgtcagac aatcgtcgta cgaaaattgc
     3661 acaattcgta tctctagcag taggtttatt ctcaattggt gtttccattg tcatggctca
     3721 ctcagatatt aaatctgcat acgaatggtt caatagtttc atgggacttg tacttggtct
     3781 acttggtggt gtatttattc ttggatttgt ttctaaaaaa gcaaataaac aaggtgctta
     3841 tgcagcgctg attgtatcaa ccatcgtcat ggtatttatt aaatacttcc ttcctccaac
     3901 agctgttagc tactgggcat attcattgat ttcaatctct gtatcagtag tttcaggtta
     3961 tattgtatct gttcttactg gaaataaagt atctgcacct aaatatacaa cgattcatga
     4021 tattacagaa attaaagcgg attcaagttg ggaagttcgt cactaataga aaagagaaaa
     4081 taatatgatt atttctgaac aatctgattt taaacgatat gcttctgtca ataaatattt
     4141 ttcaaaagtc tgtgattttt tagaaaatac aaatttgaca gatttagttg atggaaaaat
     4201 tgatattgac ggggaaaatg tttttgcaaa ttgtatgact tatctagctg atggagttcc
     4261 aggagatatt tttgaaactc ataagaaata tttagatatt catattgttg ttgaaaatac
     4321 tgaaaaaatg gcagtaactt ccccagttcg tgcccagtca cgtgtacctt ttagcgaaga
     4381 gaaggatatt gctttttacg atagcaaaga ctatcaaatt gttgaattgc ttccagggaa
     4441 tatgttggtg acttttgaag aagatcttca ccaaccaaag attcattgca atgatgaaac
     4501 tgttaaaaaa cttgttatca aagttttaaa cgaggaaaaa taaaatgaaa aaaaatatta
     4561 aacaatatgt aaccttaggt actgtagtgg tattatcagc atttgttgct aactcagttg
     4621 cagctcagga gactgaaact tctgaagtat caacaccaaa gttggtgcaa cctgttgcac
     4681 caacgactcc gatttcggaa gtacaaccta catcggataa ctcttcggaa gttactgtac
     4741 aacctcgaac agttgaaact actgttaagg atccatcttc tacagcggaa gaaactcctg
     4801 tcttagaaaa aaataatgtt actttaacag ggggcggaga aaatgttact aaagagttaa
     4861 aggataaatt tactagcggt gactttactg tagtgattaa gtacaatcag tcaagtgaga
     4921 aaggcttaca agctctgttt ggaatatcta attccaaacc cggtcaacaa aatagttatg
     4981 tagatgtgtt ccttagagac aatggtgagt tggggatgga agcgcgtgat acttcttcca
     5041 ataaaaataa cctagtatcc agacctgctt cagtttgggg taagtacaaa caagaggctg
     5101 tgactaacac tgttgcagta gtagcagatt cagtcaaaaa aacatattct ttatacgcaa
     5161 atggtacaaa agtagtagaa aagaaagtgg ataatttcct aaacatcaag gatattaaag
     5221 gtattgatta ctatatgctt gggggagtga aacgtgcagg aaaaacggcg tttggtttta
     5281 acggaacact agaaaatatc aaattcttta atagtgcatt ggatgaagaa actgttaaaa
     5341 agatgacaac aaacgctgtt actggacatt taatttatac ggctaatgat acaacaggtt
     5401 ctaactattt ccgtattcca gttctgtata cttttagcaa tggtcgggta ttttcaagca
     5461 ttgacgctcg ttacggtgga actcatgatt tcttgaataa aattaatatt gctacaagtt
     5521 atagtgatga taatggtaag acatggacta aaccaaaatt aacattggca ttcgatgatt
     5581 ttgcgccagt accattagaa tggcctcgtg aagttggtgg acgtgactta caaatcagcg
     5641 gtggtgcaac ctatattgac tctgttattg ttgaaaaaaa gaacaaacaa gtactcatgt
     5701 ttgctgatgt gatgcctgct ggagtaagtt ttagagaagc aactagaaaa gattcaggtt
     5761 ataaacaaat tgatggtaat tattacctta aattaaggaa acaaggtgat actgattaca
     5821 attatactat tcgtgagaat ggtactgtat acgacgatcg taccaacaga ccaactgaat
     5881 tttcagtaga taaaaatttc ggtattaaac aaaatggtaa ttatttgacg gtagagcaat
     5941 attcggtttc atttgaaaat aataaaaaga ctgaatatcg taatgggact aaagtccata
     6001 tgaatatctt ctacaaagat gcattgttta aagtagtgcc aacgaactat attgcatata
     6061 tttctagcaa tgatcatggc gaatcttggt ctgcaccaac tttattacct cctataatgg
     6121 gacttaatcg gaatgcgcca tatttaggtc ctggacgtgg aatcattgaa agctcaactg
     6181 gacgtattct tattccgtct tacactggta aagagtctgc gttcatttat agtgacgata
     6241 atggagcatc ttggaaagtt aaagtagtgc cacttccttc tagttggtca gcagaagcac
     6301 aatttgtaga attgagtcca ggagtaattc aagcatatat gcgtacaaat aatggtaaaa
     6361 ttgcatattt aacaagtaaa gacgcaggta ctacttggag tgcaccggaa tatttgaaat
     6421 ttgtttcaaa tccaagttat ggaacacaat tatcaatcat caattatagc caattgattg
     6481 atggtaaaaa ggctgtcatt ttaagtactc caaactccac aaatggtcgt aaacacggac
     6541 aaatttggat tggtctaatt aatgatgata atacaattga ttggcgttat catcacgacg
     6601 ttgattatag taactatgga tactcatatt caacattgac agagttacca aatcatgaaa
     6661 ttggattgat gtttgaaaaa tttgattcat ggtctcgtaa tgaacttcat atgaaaa
//
LOCUS       DAVMEI010000091         6662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVMEI010000091 DAVMEI010000000
VERSION     DAVMEI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.091"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..191
                     /locus_tag="VYQ24_001434"
     CDS             <1..191
                     /locus_tag="VYQ24_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1968561.1"
                     /translation="KEKIVNALQLPYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRI
                     FIALNIKKERTNFVLSRA"
     gene            complement(246..1787)
                     /locus_tag="VYQ24_001435"
     CDS             complement(246..1787)
                     /locus_tag="VYQ24_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1968562.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(1789..2271)
                     /locus_tag="VYQ24_001436"
     CDS             complement(1789..2271)
                     /locus_tag="VYQ24_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1968563.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNVLNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(2271..3173)
                     /locus_tag="VYQ24_001437"
     CDS             complement(2271..3173)
                     /locus_tag="VYQ24_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HEV1968564.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFADEGVQVNLAVSLHA
                     PNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAE
                     LLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGTDID
                     AACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(3194..3724)
                     /locus_tag="VYQ24_001438"
     CDS             complement(3194..3724)
                     /locus_tag="VYQ24_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1968565.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            complement(3882..4487)
                     /locus_tag="VYQ24_001439"
     CDS             complement(3882..4487)
                     /locus_tag="VYQ24_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1968566.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(4628..5665)
                     /locus_tag="VYQ24_001440"
     CDS             complement(4628..5665)
                     /locus_tag="VYQ24_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1968567.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKAFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(5699..6634)
                     /locus_tag="VYQ24_001441"
     CDS             complement(5699..6634)
                     /locus_tag="VYQ24_001441"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1968568.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFTYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT         1936 a         1483 c         1248 g         1995 t
ORIGIN      
        1 acaaagaaaa aatcgtcaac gctcttcaat taccttattc caacgcaaaa ttggaagcga
       61 ccaataatct catcaaactt atcaaacgaa acgcctttgg atttcggaac tttgaaaact
      121 tcaaaaaaag gatttttatc gctctgaaca tcaaaaaaga aaggacgaat tttgtccttt
      181 ctcgagctta gctgacttca acccactaca gttgacaaag agccgtattt cttttactct
      241 cattcttaag ttaaattatt aaaatcccat atttggtcta tccaaccttc ataaaagtct
      301 ggctcgtggc agaccataag gatagatccc ctgtattctt tgagagcgcg tttgagctca
      361 tcctttgcat ccacatccaa atggttggtc ggctcgtcca gcactaaaac gttgttttca
      421 cgattcatca agagacagaa acgaaccttg gcttgctctc cccctgataa tacttgaatc
      481 tggctttcaa tatgtttggt tgtcaaacca caacgggcaa gggctgcacg gacttctgct
      541 tgattaaggg caggaaaggc attccagaca gcttcaagag gagtttggcg attaccacct
      601 tctacttcct gctcaaaata accaagttct aaataatctc cacgctccac ttccccagcg
      661 attggcgaga taatgcccaa gagactcttc aagagagttg tttttccaat accattagca
      721 ccaataatcg caaccttttg attgcgttcg aaggtaagat ttaaaggctt agtaagagga
      781 cggtcgtaac caatttgcaa gttcttggct tggaagataa agcgccctgg tgtacgagct
      841 ggtttgaaat caaaggatgg ttttggtttc tcactttgga gttcgataat atccatctta
      901 tccaatttct tttgacgaga catagccata ttacgagttg caacacgggc tttattacga
      961 gccacaaagt ccttgaggtc tgcaatctct ttctgctggc gttcgtaggc tgcctctagc
     1021 tgagatttct tcatagcata aacttcttgg aactggtagt agtcaccaga gtaacgcgtc
     1081 agctgttgat tttccacatg atagacaata ttaataacgt cattgaggaa tggaatatcg
     1141 tgcgaaatga gaacaaaggc attctcatag ttttggagat agcgcttgag ccaatcaata
     1201 tgctcagcat ccaagtagtt ggtcggctcg tccaacagca agatatcagg cttttcaagg
     1261 agaagttttg ccaaaagcac cttggttctt tgcccacctg acaaagaagt tacatccgta
     1321 tccatgccaa agtccataac accaagagca cgcgctactt cgtcaatctt agcatccaag
     1381 gtatagaaat cacgactctc cagacggtct tgaagttctc ctacttcttc catgagagca
     1441 tcaacatccg cgccgtcttc agccattttc atatagaggt cattgatacg agcttcagct
     1501 ttgaaaagct catcaaaagc cgtacggaga acatcacgca ccgactgtct ttcagcaagg
     1561 acagagtgct gatccaagta accagccgtc acatatttgg accactcaac ctttccttca
     1621 tctggcagca ttttaccagt cacgatactc ataaaggttg attttccttc accattggca
     1681 ccgaccaggc cgatatgttc tcccttgagg agacggaagg acacatcttc aaaaattgca
     1741 cggtcaccaa aaccgtgact cagattttta acttctaaaa tactcatttt aattccttat
     1801 cttgttttta tgtaatcgtt tataaaggag ccaagccaga tagccaccca aagtattggt
     1861 ccacaaatca tcaatctcaa agacgcgatt gaaatcaaag aaaaagtcca agattaattg
     1921 cgtacactcg attccaagac tcacaagaaa actaaaaaga aggacctttt ttgttttccg
     1981 caaatttgga aatagataaa ggagttggaa aatcagagga aaaaacaaga agacattgag
     2041 gacattttgt aaaaaaatcc aacataattg tccaatgtca ctcacttcgc ccagtttcca
     2101 gagagaattg aaaggagtca aaagaaaaac caggcgtcca agatgctgaa tacctggagt
     2161 tcccactccc acggtagatt gttcttgagg agtaaagcaa aaacagacaa tgcaaatgct
     2221 atagaaaatg actccccaga ccaaaatatg attataagtc ttcttcatca ttaaggattt
     2281 accgctgcga ctgccttctg gcggtcacgt ttcattgtgt tagagcgcaa ttgtccacaa
     2341 gctgcgtcaa tatctgtacc atgctcttga cgaaccacac agttgacccc ttttttctta
     2401 agcgtatcat agaaagccaa cacgcgctct ttgggactac ggctatattg gtcatgctca
     2461 ctaactgggt tataaggaat caagtttaca taagacaatt tcttgatgtt cttgagcaat
     2521 tcagccaatt ccaaggcttg ttctacaccg tcgttgactt cattaagcat gatatattca
     2581 aaggttacac gacggtttgt tgtctcaatg tagtattcaa tagcagcaaa gagtttttca
     2641 atcggaaagg cacggttaat cttcatgata cttgaacgaa gttcattgtt gggtgcgtga
     2701 agagacacgg caagattgac ctgaacccct tcatcagcaa agtatttctg aaccagcatg
     2761 atttgcgcta cgatttcacc gttattgagg tcacgctgct tcttaatcaa accagaggca
     2821 cagaaggtac aaccgatatt acagccgacc tgagtggtca cacagacaga taaaccatag
     2881 tgttgacgca tgagtacagt ctcaattaac ataccgtcag gcaattcaaa gagatatttg
     2941 actgtaccat cagcagactc ttgcacaata cgttgtttca agggattgac cacaaactgg
     3001 tcattgagct tagcaatcaa atccttggaa aggttggtca tttcttcaaa tgactgcaca
     3061 cgtttacggt agagccattc ccagatttga tctgcacgga atttcttttc tccctgctcc
     3121 aatacccatt cctgcatggt ttgatgtacc aaactatgaa ttgagggttt catttcttct
     3181 ccttattctc tactcacttc tgacgaatga caaaatgacg ttgttccttg tcctctttct
     3241 gagaatgctg gtctttctga cgacgtctat ttttcttatc tgcattcgac tttcgtttag
     3301 tttgagtcgg tttctttcct tttctagaag gtgtttcttc ttccgtctta cgcattttct
     3361 tgtcaaatga tgctcgctta ggggcttcat tttctaagac aaaataggca caaccataac
     3421 tacaatactc taaaaggtag tcttgtaaac gactgatttt ttcaagtttt tcttctgttc
     3481 ggtcatcctt gtaaaaacct cgtaggcgaa gctgttcgtt gctccagtcc cccacgatat
     3541 aatcaaactt ggttaagact tctgaaaaac gctgattaaa agtcgtcaca tcaaaggcat
     3601 ccttgatatt ttcaaccaag gaaaaagcta tcccttccgt ttcgaccttg tccccgtgta
     3661 aatggaactc cggaccagga aacttgttat agttgtataa ttcaggtgca atttcttttc
     3721 gcatagatat cctttttcca cgattactta atactttatt ctaccataat ttctagcagt
     3781 tagcacgttt ctcataaaaa atgaaaaaag tctaacgatt ttgtcagacc agaatcttat
     3841 aacctaaaaa agagaagaac aattcttccc tccaactatc attatttagc agctgcgtac
     3901 aattcatcta ctttattcca gttgattact gaaaagaaag ctttgatgta gtcaggacgc
     3961 acgttgcggt atttcacgta gtaagcatgt tcccaaacgt ccaagcccaa gattggtttt
     4021 ttaccttctg agattggtgt gtcttggttt gctgttgaag tcacttcaag tttcccttct
     4081 ttgttgacaa ccaaccatgc ccaacctgaa ccaaaacgag ttgttgctgc tgcagtgaag
     4141 gctgcttgga attcttcaaa tgaaccaaat gttgcatcga ttgctgctgc cagttctgct
     4201 gaaggagctg ttttctcggg agtcatcaat tcccagaaaa gagcgtggtt caagtgtccg
     4261 ccaccattgt tgataagtgc ttgacggata tcagctggga tagattctac atcagcaagc
     4321 aaggcttcaa ggtcttcacc gatttcaggg tgtttttcta aagctgcatt ggcattgttg
     4381 acataagttt gatggtgttt gtcatggtgc aaatgcattg tttccgcatc gatgtatggt
     4441 tccaaagcgt cgtatgcata tggaagttct ggtaagataa tagccatctg taatacctct
     4501 ttttctttct atatgaaaat gataacgcaa acattcactt ttttcaagtt ttttgcttat
     4561 agataagcaa atcagcaaga aacccacgac aagataatcc tgacgcgggt tgtagtttca
     4621 acaaatgtca attgacctga ctagcaatct gtaagagtgc cttttcaaaa aggaaacctt
     4681 tttcataaag acctgtctta atctgatagt ctgtctcaat caaataggaa atagcttgct
     4741 tcagaaagct caaagaaagt cctctcgaat ctcttaatgc aaacttgatt tgataaggat
     4801 ttgggttacg tcccagataa ctacctaaac tacttgcaat ctgcgattct gtttggccag
     4861 actccgccaa aatcttcacc tgagtaaaag tccgaaattg tcccagcatg actgcaatta
     4921 gtttgatttc atcttcccct tgcaaggtca agtctctcac caaatcgcgc gcctgatcca
     4981 tctttttagt cagaataaac tgagttaaat caaaaatatt gtcctgcaag gtcttgggaa
     5041 ttgcgttaac aatatcctct tcctcgataa cagaatccgc cttataggac tgtaaaaaga
     5101 gaagattttt ctggatttcg ctaaattgaa accccgactt gatgaggaga ttttcaaaag
     5161 aatgattggt aaactgcaga ccttgtttct gactccactt ttggaaatac tggcgcaatt
     5221 cttgttcttt tacttctact gcatcgaagg ccttggcatc acgcttaagt aatttgacta
     5281 accgtctttt gctatccagc tttccttctg caaagattat caacttggtt gttggagaag
     5341 gattgtcaag gtattcctca aatgacttaa gctcatcatc tgtcaaaaag cgtttcttag
     5401 cagtcgtgat atccataaaa taatctaata tcacgatttt ctcatccgca aagaaaggaa
     5461 ggctgaccaa ctccagttcc acatccttgt aaactacttc tttcatatca aagtaggcaa
     5521 agttgaggtc agcagaatca tacccaatct gtttcaacac ttgactcttc atcacttcaa
     5581 actgaccctg atctgtccct gtaaataggc tcaggctcgg taaatttgat aaagtcaact
     5641 tctgactttc ttcaatggct agcatcgtct ctcctttctt cagatttttc gatttaattt
     5701 agtcaatata gcgcaatttc ccacggaaat cttctaagct ctcgtagcct ttttccacca
     5761 tgattgcttt cagttcattg gtaatgcggt caaaagcact gacgccttct ttgtgaaggg
     5821 tcgttcccac ctgcaccata cttgctccac agaggatgtg ttcaaaggca tctcgaccag
     5881 tcagaacgcc acctgttccg ataatttgga tttgaggatt taaacgttga taaaaggcgt
     5941 gaacattggc tagagcagtc ggtttgatgt attctccacc aattccacca aaaccattct
     6001 taggccgaat aacgacagat tcgtcttcta tatagaggcc gtttccgata gagttaacgc
     6061 agttgacaaa cttgagcgga tatttgttga aaatagctgc cgcttgatca aagtgaacaa
     6121 tatcaaaata aggtggcaat ttaattccaa gaggtttggt gaagtaagta aacacttctg
     6181 ccaaaatccg gtctgttgtc tcaaaatcat aggcaatctg aggtttacct ggaacatttg
     6241 gacaggaaag atttagctca gtcagaccac gaaaatcact ctcttgaact tttttcaaaa
     6301 tagtatgggt ttcctctgga gacatgccga ccagagataa gaagaaagtt cggttcgact
     6361 ctttttcctg caaatctaaa agataatcca aataatagtc taagccatta tttggcaagc
     6421 ccatagagtt gatggaacca agtggaacat cttggtagcg tggctcagga ttcccctgac
     6481 ggaagtccaa ggtcgctgtc ttagtaacaa aggttcctgc cgctgagttt ttgacctctt
     6541 ctaactcctc tatcgtcata caagccacac ctgctgcatt catcaagcaa ttgtcaaact
     6601 caaaaccagc aatttgtgtt ttcgttgata ccatgattct aatcctccag atttctttta
     6661 tt
//
LOCUS       DAVMEI010000092         6658 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVMEI010000092 DAVMEI010000000
VERSION     DAVMEI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.092"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1077
                     /gene="dnaN"
                     /locus_tag="VYQ24_001442"
     CDS             <1..1077
                     /gene="dnaN"
                     /locus_tag="VYQ24_001442"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1968569.1"
                     /translation="AISSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNED
                     AGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQ
                     YPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVAT
                     DSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENI
                     SFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKD
                     GVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAV
                     RPFTLVPADTDEDFMQLITPVRTN"
     gene            1142..1336
                     /locus_tag="VYQ24_001443"
     CDS             1142..1336
                     /locus_tag="VYQ24_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1968570.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1420..2535
                     /gene="ychF"
                     /locus_tag="VYQ24_001444"
     CDS             1420..2535
                     /gene="ychF"
                     /locus_tag="VYQ24_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1968571.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2607..3176
                     /locus_tag="VYQ24_001445"
     CDS             2607..3176
                     /locus_tag="VYQ24_001445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1968572.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3177..>6658
                     /gene="mfd"
                     /locus_tag="VYQ24_001446"
     CDS             3177..>6658
                     /gene="mfd"
                     /locus_tag="VYQ24_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1968573.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIVASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIHDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEI"
BASE COUNT         2253 a         1021 c         1359 g         2025 t
ORIGIN      
        1 gctattagtt ctaaaaatgc cattcctatt ttatcaacag taaaaattga cgtgaccaat
       61 gaaggtatta ctttaattgg ttcaaatggt caaatttcaa ttgaaaattt tatttctcaa
      121 aaaaatgaag atgctggttt gttaattact tctttaggtt cgatccttct tgaagcttct
      181 ttctttatca atgtagtatc tagtttacct gatgtgactc ttgattttaa agaaattgaa
      241 caaaatcaaa ttgttttaac cagtggcaaa tcagaaatta ccctaaaagg aaaagatagc
      301 gaacaatatc cacgaatcca agaaatttca gcaagcactc ctttaatact tgaaacaaaa
      361 ttactcaaga aaattattaa tgaaacagcc tttgctgcaa gtacacaaga gagtcgtccg
      421 attttaacag gtgtccactt cgtattgagt caacacaaag agttaaaaac agttgcaaca
      481 gactctcatc gcctaagcca gaaaaaattg actcttgaaa aaaatagtga tgattttgat
      541 gtcgtaattc ctagccgttc tctacgcgaa ttttcagcgg tatttacaga tgatatcgaa
      601 actgtagaga ttttctttgc caataaccaa atcctcttta gaagcgaaaa tattagcttc
      661 tatactcgtc tcctagaagg aaactatcct gatacagatc gcttgattcc aacagacttt
      721 aacactacta ttacttttaa tgtggtaaac ttacgccagt caatggagcg tgcccgtctt
      781 ttatcaagtg cgactcaaaa tggtactgtg aaacttgaaa ttaaggatgg ggttgttagc
      841 gcccatgttc actctccaga agttggtaaa gtaaacgaag aaatcgatac tgatcaggtt
      901 actggtgaag atttgaccat tagtttcaac ccaacttact tgattgattc tcttaaagct
      961 ttaaatagcg aaaaggtgac cattagcttt atctcagctg ttcgtccatt tactcttgtg
     1021 ccagcagata ctgacgaaga cttcatgcag ctcattacac cagttcgtac aaattaagtg
     1081 aaagaggttg agcctggctc gcctctttta tgatataatc gaaaaagaaa aggagagtag
     1141 tatgtatcaa gttggaaatt ttgttgagat gaaaaaatca cacgcttgta caatcaagtc
     1201 gactggtaaa aaggctaatc gttgggaaat tacacgtgta ggagcagata tcaaaataaa
     1261 atgcagtaat tgtgagcatg ttgtcatgat ggggcgatat gattttgagc gaaaaatgaa
     1321 taaaattatt gactgagaac ccttagttag agggttagcg ctttatccct ttttgtgtta
     1381 taatattagg gattgaaatg aaaacggaga atgagaaata tggctttgac agcaggtatc
     1441 gttggtttgc caaacgttgg taaatcaaca ctatttaatg caattacaaa agcaggagca
     1501 gaggcagcaa actacccatt tgcgacgatt gatccaaatg ttggaatggt ggaagttcca
     1561 gatgaacgcc tacaaaaact aactgaaatg ataactccta aaaaaacagt tcccacaaca
     1621 tttgaattta cggatattgc agggattgta aaaggagctt caaaaggaga agggctaggg
     1681 aataaattct tggccaatat tcgtgaagta gatgcgattg ttcacgtagt tcgtgctttt
     1741 gatgatgaaa atgtgatgcg cgagcaagga cgtgaagacg cctttgtaga tccacttgca
     1801 gatattgata caattaatct ggaattaatt cttgctgact tagaatcagt gaacaaacga
     1861 tatgcgcgtg tagaaaagat ggcacgtacg caaaaagata aagaatcagt agcagaattc
     1921 aatgttcttc aaaagattaa accagtccta gaagacggga aatcagctcg taccattgaa
     1981 tttacagatg aggaacaaaa ggttgtcaaa ggtcttttcc ttttgacgac taaaccagtt
     2041 ctttatgtag ctaatgtgga cgaggatgtg gtttcagaac ctgactctat cgactatgtc
     2101 aaacaaattc gtgaatttgc agcgacagaa aatgctgaag tagtcgttat ttctgcgcgt
     2161 gctgaggaag aaatttctga attggatgat gaagataaaa aagagtttct tgaagccatt
     2221 ggtttgacag aatcaggtgt agataagttg acgcgtgcag cttaccactt gcttggattg
     2281 ggaacttact tcacagctgg tgaaaaagaa gttcgcgctt ggactttcaa acgtggtatg
     2341 aaggctcctc aagcagctgg tattatccac tcagactttg aaaaaggctt tattcgtgca
     2401 gtaaccatgt catatgaaga tctagtgaaa tacggatctg aaaaggccgt aaaagaagct
     2461 ggacgcttgc gtgaagaagg aaaagaatat atcgttcaag atggcgatat catggaattc
     2521 cgctttaatg tctaaaaatt aataaatggt gtcaattagg ttggaaaaaa aattccaacc
     2581 cttttggctt ttgaaaggaa aaataaatga ccaaattact tgtaggcttg ggaaatccag
     2641 gggataaata ttttgaaaca aaacacaatg ttggttttat gttgattgat caactagcga
     2701 agaaacagaa tgtcactttt acacacgata agatatttca agctgaccta gcatcctttt
     2761 tcctaaatgg agaaaaaatt tatctggtta aaccaacgac ctttatgaat gaaagtggaa
     2821 aagcagttca tgctttatta acttactatg gtttggatat tgacgattta cttatcattt
     2881 acgatgatct tgacatggaa gttgggaaaa ttcgtttaag agcaaaaggc tcagcaggtg
     2941 gtcataatgg tatcaagtct attattcaac atataggaac tcaggtcttt aaccgtgtta
     3001 agattggaat tggaagacct aaaaatggta tgtcagttgt tcatcatgtt ttgagtaagt
     3061 ttgacaggga tgattatatc ggtattttac agtctattga caaagttgac gattctgtaa
     3121 actactattt acaagagaaa aattttgaga aaacaatgca gaggtataac ggataaatgg
     3181 tgaccttatt agatttattc tcagaaaatg atcagattaa aaaatggcat caaaatttaa
     3241 cagataagaa aagacaacta atacttggtt tatcaacatc tactaaggct cttgcaattg
     3301 caagcagttt agaaaaagaa gataggattg tgttattgac gtcaacttat ggagaagcag
     3361 aaggacttgt tagtgatctt atatctatct tgggtgagga actcgtctat ccatttttgg
     3421 tagatgatgc tcctatggtg gagtttttga tgtcttcaca ggaaaaaatt atttcacggg
     3481 ttgaagcctt gcgttttttg actgattcat ctaagaaagg gattttagtt tgtaatatcg
     3541 tagcaagtcg attgatttta ccgtctccca atgcattcaa agatagtatt gtaaaaatct
     3601 cagttggtga agaatatgat caacacgcgt ttatccatca gttaaaggaa aatggctatc
     3661 gaaaagttac tcaagtacaa actcagggcg aatttagtct tcgaggagat attttagata
     3721 tttttgaaat atcccagtta gaaccttgtc gaattgagtt ttttggtgat gaaattgatg
     3781 gtatcaggtc atttgaagta gaaacacaat tatcgaaaga aaataagaca gaactcacta
     3841 tctttccagc tagtgatatg cttttgagag aaaaggatta tcaacgagga cagtcagctt
     3901 tagaaaaaca aatttcaaaa actttatcac ctattttgaa atcataccta gaagaaattc
     3961 tttcaagttt tcaccaaaaa caaagtcatg cagactctcg gaagttttta tctttgtgct
     4021 atgataagac atggactgtc tttgattata ttgaaaaaga taccccaata ttctttgatg
     4081 attatcaaaa attgatgaat cagtatgaag tctttgaaag agacttagcg cagtacttta
     4141 cagaagaatt acagaatagt aaagcatttt ctgatatgca gtatttttct gatattgaac
     4201 aaatctataa aaaacaaagt ccagtgacct ttttctctaa tcttcaaaag ggtttaggaa
     4261 atctcaaatt tgacaaaatt tatcaattca atcaatatcc tatgcaggaa tttttcaatc
     4321 agttttcttt tctaaaagaa gaaattgaac gatataaaaa aatggattac accattattc
     4381 tgcagtctag caattcaatg ggaagtaaaa cattggagga tatgttagag gaatatcaga
     4441 ttaaattgga ttctagagat aagacaaata tctgtaaaga atctgtaaac ttaatagagg
     4501 gtaatctcag acatggtttt cattttgtag atgaaaagat tttattgata actgaacatg
     4561 agatttttca aaagaaatta aagcgtcgtt ttcgaagaca acatgtttca aatgcagaga
     4621 gattaaaaga ttacaatgaa cttgaaaaag gggactatgt tgtccatcat atccatggga
     4681 ttggtcaata tctaggaatt gaaaccattg aaatcaaggg aattcatcgc gattatgtca
     4741 gtgtccaata ccaaaatggt gatcaaattt ctatccccgt ggaacagatt catctactgt
     4801 ccaaatatat ttcaagtgat ggtaaagctc caaaactcaa taaattaaat gacggtcatt
     4861 ttaaaaaggc caagcaaaag gttaagaacc aggtagagga tatagctgat gatttaatca
     4921 aactctactc tgaacgtagt cagttgaagg gttttgcttt ctcagctgat gatgatgatc
     4981 aagatgcctt tgatgatgct ttcccttatg ttgaaacgga tgatcaactt cgtagtattg
     5041 aggaaatcaa gagggatatg caggcttctc agccaatgga tcgactttta gttggggatg
     5101 ttggttttgg aaagactgaa gttgctatgc gtgcagcctt taaagcagtc aatgatcaca
     5161 aacaggttgt cattctagtt ccgacgacgg ttttagcgca acagcactat acgaatttta
     5221 aggaacgatt ccaaaatttt gcagttaata ttgatgtgtt gagtcgcttt agaagtaaaa
     5281 aagagcagac tgcaacactt gaaaaattga aaaacggtca agtcgatatt ttgattggaa
     5341 cacatcgtgt tttgtcaaaa gatgttgtgt ttgctgattt gggcttgatg attattgatg
     5401 aggaacagcg atttggtgtc aagcataagg aaactttgaa agaactgaag aaacaagtgg
     5461 atgtcctaac cttgaccgct acgccaatcc ctcgtaccct ccatatgtct atgctgggaa
     5521 tcagagattt atctgttatt gaaactccgc cgactaatcg ctatcctgtt cagacctatg
     5581 ttttggaaaa gaatgatagt gtcattcatg atgctgtctt gcgtgaaatg gagcgtggag
     5641 gtcaagttta ttatctttac aacaaagttg acacaattgt tcagaaggtt tcagaattac
     5701 aggagttgat tccggaggct tcgattggat atgttcatgg tcgaatgagt gaagtccagt
     5761 tggaaaatac tctattagac tttattgagg gacaatacga tatcttggtg acgactacta
     5821 ttattgagac aggggtggac attccaaatg ctaatacttt atttattgaa aatgcggacc
     5881 atatgggctt gtcaacctta tatcagttaa gaggaagagt cggtcgtagt aatcgtattg
     5941 cttatgctta tctcatgtat cgtccagaaa aatcaatcag tgaagtctct gaaaagagat
     6001 tagaagcgat taaaggattt acagaattgg gctctggctt taagattgca atgcgagatc
     6061 tttcgattcg tggagcagga aatcttttag gaaaatccca gtctggtttc attgattctg
     6121 ttggttttga attgtattcg cagttattag aggaagctat tgctaaacga aacggtaatg
     6181 ctaacgctaa cacaagaacc aaagggaatg ctgagttgat tttgcaaatt gatgcctatc
     6241 ttcctgatac ttatatttct gatcaacgac ataagattga aatttacaag aaaattcgtc
     6301 aaattgacaa ccgtgtcaat tatgaagagt tacaagagga gttgatagac cgttttggag
     6361 aatacccaga tgtagtagcc tatcttttag agattggttt ggttaaatca tacttggaca
     6421 aggtctttgt tcaacgtgtg gaaagaaaag ataataaaat tacaattcaa tttgaaaaag
     6481 tcactcaacg gctgttttta gctcaagatt attttaaagc tttatccgta acgaacttaa
     6541 aagcaggcat cgctgagaat aagggattaa tggagcttgt atttgatgtc caaaataaga
     6601 aagattatga aattttagaa ggtctgctga tttttggaga aagtttatta gagataaa
//
LOCUS       DAVMEI010000093         6606 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVMEI010000093 DAVMEI010000000
VERSION     DAVMEI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.093"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            270..1130
                     /gene="speE"
                     /locus_tag="VYQ24_001447"
     CDS             270..1130
                     /gene="speE"
                     /locus_tag="VYQ24_001447"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1968574.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            1127..2386
                     /locus_tag="VYQ24_001448"
     CDS             1127..2386
                     /locus_tag="VYQ24_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1968575.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            2386..3513
                     /gene="nspC"
                     /locus_tag="VYQ24_001449"
     CDS             2386..3513
                     /gene="nspC"
                     /locus_tag="VYQ24_001449"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1968576.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            3510..4595
                     /gene="aguA"
                     /locus_tag="VYQ24_001450"
     CDS             3510..4595
                     /gene="aguA"
                     /locus_tag="VYQ24_001450"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1968577.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPVVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVAIDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            4605..5480
                     /gene="aguB"
                     /locus_tag="VYQ24_001451"
     CDS             4605..5480
                     /gene="aguB"
                     /locus_tag="VYQ24_001451"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1968578.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(5661..6470)
                     /locus_tag="VYQ24_001452"
     CDS             complement(5661..6470)
                     /locus_tag="VYQ24_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1968579.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         1929 a         1310 c         1483 g         1884 t
ORIGIN      
        1 cctacataaa gcatgggagt acctccagat atagtatttc aagtctactt ggttatccac
       61 gaactttttg ccttgttacc ttagacgaga taaaacgtct atgcgttatc aaactcatta
      121 ccaattgaaa caaaaagctg tggttagagc ctttcggaaa tcgtcaagcg attggaggaa
      181 atgaactaat ccacagtgac ttattccaag tataccactt gggctttggc agtagctaac
      241 tgcgctaaat ataatataag gagaaataga tggatttatg gttttctgaa gttcatactc
      301 cagatgtcaa attgtctctg agaacagcca agcaacttta cgctggaaaa agtgaatggc
      361 aggatatcga agtcttggat acgccagctt ttgggaaaat actgatttta aatggccatg
      421 tcttgttctc agatgcggat gatttcgtct acaatgaaat gaccgtccac gttcccatgg
      481 ctgtccaccc aaatccaaag aaagtattgg ttattggggg tggtgacggc ggtgttgccc
      541 aagtattaac actctatcct gaactggaac aaatcgatat cgtggaaccg gatgagatgt
      601 tggtcgaggt ctgtcgtgag tatttcccag actttgctgc agggctagat gatcctcgtg
      661 ttaccattta ctaccaaaat gggctacgct ttttgcgaaa ctgcgaagat gattacgata
      721 ttatcatcaa cgatgcgaca gatccatttg gccatacgga aggactcttt accaaggaat
      781 tctacggcaa tagttatcga gctctgaagg aagacggcat catgatttac cagcatggga
      841 gtcccttctt tgacgaggat gagtcggcct gccgaagcat gcaccgcaag gtcaatcaag
      901 cctttccaat cagtcgggtt tatcaggccc atattccaac tagcccagct ggctattggt
      961 tgtttggatt tgcatcgaaa aaataccacc ctgtcaaaga ttttgacaag gaaggttgga
     1021 aaaaacgcca gcttttcaca gaatactaca ctgcaaactt acacgtggga tcctttatgt
     1081 tgcccaagta tgttgaggac attttagaag aagaggaagg aaaaaaatga gtcgtttact
     1141 agttattggt tgtgggggcg ttgcccaagt tgctatttca aagatttgtc aagatagcga
     1201 aacatttaca gagattatga ttgctagccg taccaagtca aaatgcgatg acttgaaagc
     1261 gaagctagaa ggcaaaacaa gtactaaaat tgaaactgca gcacttgatg ctgacaaggt
     1321 tgaagaagtg attgccctga ttgaaagcta caaaccagaa gctgttttga atgtagctct
     1381 gccttatcaa gatttaacca ttatggatgc ttgtttggca acaggtgttc actatatcga
     1441 tacagccaac tacgaagcag aagacacaga agaccctgag tggcgtgcta tctacgaaaa
     1501 acgttgtaag gaacttggtt ttacagccta ctttgactac tcatggcagt gggcttatca
     1561 agagaaattc aaagaagcag gcttgactgc tcttcttggt tctggttttg acccaggtgt
     1621 aactagtgtc ttttcagctt atgccctcaa acactatttt gatgaaatcc attatatcga
     1681 cattttagac tgtaatggcg gtgaccacgg ttatccattt gcaaccaact ttaatccaga
     1741 aattaatctc cgtgaggttt ctgcgccagg ttcttactgg gaagatggga aatgggtcga
     1801 agtcgaagct atgtctatca agcgtgagta tgatttccct caagttggac aaaaagatat
     1861 gtatctcctt caccatgaag aaatcgaatc attggccaag aacattccag gtgtcaaacg
     1921 cattcgtttc tttatgactt ttggtcaatc ttacttgacg cacatgaaat gtcttgaaaa
     1981 tgttggactc cttcgtacgg ataccattaa ctttaacggc caagaaattg ttccaattca
     2041 atttttgaaa gccttgcttc cagatcctgc tagccttggc ccacgtactg taggtaaaac
     2101 caatattgga tgtatcttta caggtgtcaa agatggcgtt gaaaagacta tctacatcta
     2161 caatgtttgc gaccatcaag agtgctatgc agaagtcggg tcacaagcaa tttcatacac
     2221 gacaggagtt ccagccatga ttgggacaaa attagtcatg aacggaactt ggaaacaagc
     2281 tggagtgtat aaccttgagg agttagatcc agatccattc atggaagctt tgaatgagta
     2341 tggtttgcca tgggttgtgg ttgaaaatcc acaaatggtg gactaatgaa gttagaacaa
     2401 gtaccaacac cagcctatgt tattgacttg gccaagttag aagctaattg ccgcattcta
     2461 caatatgtac aagaagaggc cggttgcaag gtcttgcttg cccagaaggc atattccctc
     2521 tacaaaactt atcccttgat tagccagtat ctatcaggta cgacagctag tggactctat
     2581 gaggccaaat tggcaaggga agaatttcct ggtgaagtcc atgtatttgc gcctgctttc
     2641 aaggatgcag acttggagga attgcttggg ataacagacc atatcgtctt taactcagag
     2701 agacagttgc gtaagcatgg ggctcgttgt cgagaggctg gtgtcagtgt tggtttgcgc
     2761 ctcaaccctc agtgttcaac tcaaggagat cacgcgctct atgacccttg tgcaccaggt
     2821 tctcgctttg gagttactat agacaagatt ccgagtgatt tgctagattt ggttgacgga
     2881 cttcattttc ataccctttg cgagcaggga gcagatgatt tacaaacaac tttgaaagca
     2941 gtagaagaac aatttggtcc ctacttacat gaggtaaaat ggctcaatat gggtggtggt
     3001 catcatatta caagagaagg ttacgatgtg gatttgctga tttcagaaat caagcgtatc
     3061 cgaaaaactt acaatcttga aatctatatc gagcctggtg aagccattgc gcttaatgcg
     3121 ggttatttag caactgaggt attagatatt gtagaaaacg gtatggaaat cttggtttta
     3181 gacgcctctg cgacctgcca tatgcctgat gtacttgaga tgccctatcg tccacctttg
     3241 agaaatggct ttgaggcaca ggaaaaagcc catacctaca gactttcttc taatacctgt
     3301 ctgactggcg atgtgattgg tgattatagt tttgaaaatc cagtccaaat cggagacaga
     3361 ctttattttc aagacatggc catttattct tttgtcaaaa ataatacctt taatggtatt
     3421 ggattgccaa gtctctatct catggacgaa cagggagact gtagcttact caaagctttt
     3481 ggctatcaag actttaaagg gagattatca tgatggacag tccaaaaaaa ttaggctatc
     3541 acatgccagc agagtacgaa ccccatcatg gtaccctcat gatatggccg actcgaccag
     3601 gatcatggcc ttttcaagga aaggctgcta aaagagcatt tactcagatc atcgagacca
     3661 tagcagaagg ggaaagagtc tatcttttgg tggagcaggc ctatctatct gaagcccaat
     3721 cctatcttgg agacaaggtt gtttatttag acattcccac caatgatgcc tgggcgcgtg
     3781 atactggccc aaccattctc gtcaatgata aaggtaagaa attagccgtg gattgggcct
     3841 tcaatgcttg gggaggcacc tatgatggtc tttatcaaga ttatgaagag gatgaccaag
     3901 tagccagtcg ttttgctgag gctttggaaa ggcctgtcta tgatgctaaa ccttttgtac
     3961 tggaaggagg cgcaatccat agcgatggtc aaggaactat tctcgtaact gaaagttgct
     4021 tgcttagtcc tggtcgcaat cctaacttga ctaaagagga gattgaaaac acattattag
     4081 aaagtcttgg tgctgaaaaa gttatttggc ttccttatgg tatttatcag gatgaaacca
     4141 atgaacacgt cgataatgtt gctgcctttg ttggtcctgc tgagcttgtt ttggcttgga
     4201 cagatgacga aaatgatccc cagtatgcca tgtcaaaagc agatctcgaa ctcttagaac
     4261 aggaaacaga tgcaaaaggt tgtcacttca ccattcataa attgcctatc cctgtagttc
     4321 gacaagttgt gacagaagaa gatttgccag gctacatcta tgaagaagga gaagaagagc
     4381 gatacgcagg tgaacgacta gcagcttcct acgtaaactt ttatatcgcc aacaaggctg
     4441 tcttggttcc acagtttgag gatgtaaacg accaagtggc catagatatc ctcagcaagt
     4501 gtttcccaga ccgtaaagtt gtcggaatac cagccagaga tattctctta ggtggtggta
     4561 atatccactg tatcacccaa caaattccag aataggagaa aaagatgaga aatgtaagag
     4621 ttgcaaccat tcagatgcaa tgcgctaagg atgtggcaac aaatatccaa accgcagagc
     4681 gtttagtacg tcaggctgct gagcaaggag cccaaattat tctcttgccc gagttgtttg
     4741 aacatcccta tttctgtcag gaacgtcagt atgactacta ccagtatgcc caatctgtag
     4801 cggaaaatac tgccattcag cattttaagg tgattgctaa ggaactacaa gttgttttac
     4861 caatcagttt ctatgaaaaa gatggtaatg tcttgtataa ctctattgcc gtcattgatg
     4921 cagatgggga agtgctgggc gtttatcgaa agacccacat accagatgac cattattatc
     4981 aagaaaaatt ctatttcacg cctggtaaca ctggtttcaa ggtctggaat actcgctatg
     5041 ctaagattgg tatcggtatc tgttgggatc aatggttccc tgaaacagcg cgctgtcttg
     5101 cattgaatgg tgctgaattg ctcttttatc ctacagctat cggttcagag ccaattttgg
     5161 atacagatag ttgtggtcac tggcaacgta ctatgcaagg gcacgcagca gcgaatattg
     5221 ttccagtcat cgcagccaat cgttatggtt tagaggaggt tactcctagt gaggaaaatg
     5281 gcggacagag ttccagtctt gacttctacg gttcctcctt tatgacggat gaaacaggag
     5341 ctattctaga acgagctgaa agacaagaag aagctgttct gttagctact tatgacctag
     5401 acaagggagc aagtgaacgc ctaaactggg gcttgtttcg agatagaaga ccagaaatgt
     5461 atagacaaat tacagattag tgtgggagaa atgagagatt cattctgcta gactaacttc
     5521 ttattagcaa ctataagata ctatggcatc tagtaaatcg atttttatga ttcgctattc
     5581 ttgtctattg attagtccgt attttaaaat attagcaaaa aagcaaatag cagtaacttc
     5641 tgtctatttg cttttctttt ttatagaata tatttctcaa tagcacgcgc aacgccgtct
     5701 tcttcgttgc ttgaggtaac ggcatccgca agagatttga cataatcgct ggcatttccc
     5761 attgcaatcc caagccctgc aaactggagc atttcgatat cgttattagc atcgcccatg
     5821 gccataatct ctgaggaatc aatcttcaaa atctcagcta gtcgtgaaag agcagtagcc
     5881 tttgtcgttc caagtggcat tgcttcataa atgacaggct gcgaacgaac tccactgaat
     5941 cgttggcaaa gctcttcagc aaaacgctgc tcaaaatcgt ctgtttgttc ttttgttcct
     6001 aaaaacatac cttggaacat ccggaacttt ccactagtcg cttcctcaag agaaatttca
     6061 gtcaggtctg aaaatactag tttagcatca ttttcaataa cttgattggg cttgccaccg
     6121 agaacaaaat aatgtgactc gtcaaaaagt gtcaactgaa catcactctt ttcagcaagg
     6181 tcatagaggt attcgatgtc agctggactc agttctttcc agtcaactag accccaatca
     6241 ctggtttggt gagttgaaca accgttgtta acaataacat attcgttctg gaggtcaagc
     6301 cccagttttt tgtagtaggg gaggacaccg aaaagggggc gacccgtaca gagaaccagt
     6361 ttgacacctt tttcaatggc tttgtgaata gcagtaatgt gagcttgtgg gatttccttg
     6421 gcttcattga ggagggttcc atccatatcc aaggctagta gtttaatcat aggtcttcct
     6481 tctttatctt ttggtagtat tatagcatat tttggagaag aaattgatag aaagcttgag
     6541 actaattgat tttatagttt aagatgtttt gatgacaatt catgatttga agaggatatt
     6601 tggcaa
//
LOCUS       DAVMEI010000094         6596 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVMEI010000094 DAVMEI010000000
VERSION     DAVMEI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.094"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            13..876
                     /locus_tag="VYQ24_001453"
     CDS             13..876
                     /locus_tag="VYQ24_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968580.1"
                     /translation="MSTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIQAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELGTV"
     gene            1259..1879
                     /locus_tag="VYQ24_001454"
     CDS             1259..1879
                     /locus_tag="VYQ24_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968581.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYTGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            1965..2408
                     /gene="tsaE"
                     /locus_tag="VYQ24_001455"
     CDS             1965..2408
                     /gene="tsaE"
                     /locus_tag="VYQ24_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1968582.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2398..2916
                     /locus_tag="VYQ24_001456"
     CDS             2398..2916
                     /locus_tag="VYQ24_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968583.1"
                     /translation="MEYELLIREAEPKDAAELVTFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            2924..3940
                     /locus_tag="VYQ24_001457"
     CDS             2924..3940
                     /locus_tag="VYQ24_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1968584.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4025..5281
                     /locus_tag="VYQ24_001458"
     CDS             4025..5281
                     /locus_tag="VYQ24_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1968585.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5336..6502
                     /gene="recA"
                     /locus_tag="VYQ24_001459"
     CDS             5336..6502
                     /gene="recA"
                     /locus_tag="VYQ24_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1968586.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
BASE COUNT         2094 a         1080 c         1489 g         1933 t
ORIGIN      
        1 ttattatctt taatgagtac actcgcagag aaattcagat tgaaaagaaa agagttgaga
       61 ctctcccaac aaactcttgc agaaggaatt tgtgaacaaa gccagattag taaaattgag
      121 agagggcatt tcattccctc cgcagacctt ttgttcaaac tctctcaacg acttgaagta
      181 ccattagatt atttttttaa tgaacaaatt gaaattaaat ctaacctctc taatttcaag
      241 caattgtctg ctcgactatt agacgataga aattatgacg atttggaata tatttataga
      301 atagagattg aacgaagtac ttttctaaca ctagaagacc gaacttacct tgaatggatt
      361 caagctatta ttgacttcta tcaatatgac agtaagtgtg aggcaatttc ttcattggaa
      421 aatatattat taaaagtctc ttcaaatact ctgatttatt taaaggcgtt gaatactcta
      481 tctaatttct attccttagt gggtcgtgaa caagaatatg aggcaaacta ctctcattta
      541 atagagttgt atcagacaaa aaatttggac catcaagagt ttttatttgg ctacatcaga
      601 gttcgttaca actacgctca ctacctagta tcaaaggaaa aatataatga agccatccaa
      661 gaagctcttg aaacgattga actctgtaaa caaagacaga caagctacca attggctcct
      721 ctacttattc ttgtaggaaa tgctggagcc aaatttctag acagagaaca agtcaaaaat
      781 tattatatag aagcaagaga gttatgtaag atttataaca atcctttaat gttgatgaag
      841 atagaaaatt atttgaagga actaggtact gtttagttaa tcttgacatt ataatttttc
      901 tgaaacgtta aataacctgt aaggctgata gttagattaa tactatcagc cttataatta
      961 tgtgactctg agtccgtctc gctccgtgag gtgcgagaca aaacaaccac ccgtttgacg
     1021 gattttcatg atttcaaatt tatattcttc gaaaatctct tcaaatcacg tcagctctat
     1081 ctgcaatctc aaaacactgt tttgagcaac ctgtggctag ctacctagtt tgatctttga
     1141 ttttcattga gtattagtct ctagtgtttg tttagcgtct taaagagaca tttgagaaga
     1201 ttagaaacag ttctctcttt tctcctagta taatgattgg taggaaggga gagagaagat
     1261 gaaatcaatg agaatcttat ttttgttagc tttaattcaa atcagtttga gtagctgttt
     1321 cctatggaag gaatgcatct tgtcctttaa acaaagtaca gcttttttca tcggaagcat
     1381 ggttttcgtt tcaggaatct gtgctggagt aaattatctt tatacccgta agcaagaagt
     1441 ccatagtgtc ctagccagta agaagtcggt gaagcttttt tacagtatgt tactcttaat
     1501 taatttgtta ggagctgttc ttgttttgtc agataacttg ttcatcaaaa atacgctgca
     1561 gcaagaatta gttgactttt tattgccatc cttctttttc ctatttgggc tagatttgct
     1621 gattttttta cccttgaaaa aatacgtgcg cgattttctt gctatgctag acagaaaaaa
     1681 gacagtgttg gtgactattt tagcaacact tcttttctta agaaatccaa tgaccattgt
     1741 ctcacttctg atttatactg gactgggctt gttttttgca gcctatcttg tcccaaattc
     1801 ggttaagaag gaagtttcct tttatggtca tattttccga gatcttgtat tggtcattgt
     1861 tacgctcatt ttcttttaga gaaagcctgt tttttatgga tgttatgatg ataagtttag
     1921 taggaaaata gacacaaaag aaaagttttt ggtataataa gagtatgtac acaaaaaatg
     1981 aagaagagtt gcaagcctta ggggagcgtt tgggccatct attagcaaag aatgatgttt
     2041 taatcttaac tggagaactg ggtgcaggta aaacgacctt tactaaagga cttgcaaaag
     2101 gattacagat ttctcaaatg attaaaagtc ccacctatac tatcgtgaga gagtatgaag
     2161 gtcgacttcc actttatcac ctagatgttt atcgtattga aggagatgct gattctatcg
     2221 acttggatga gtttatcttt ggtggcggcg tgactgttat tgagtgggga aatctcttag
     2281 gagatgcctt gccagatgct tatttagaat tggaaattct aaaagaagca gatggacgcc
     2341 gtttaaattt tcaggcaaag ggtttgcgtg ctgagaaatt gttagaggag cttcaatatg
     2401 gagtatgaat tgctcattag ggaagcagag cccaaagatg cagctgaatt agtgaccttt
     2461 ttaaatcgtg tgagtttgga gacagacttt accagcctag acggagatgg tattctcttg
     2521 actagtgagg agatggaaat attcctcaac aagcaagcta gttcggacaa tcagataacc
     2581 ttacttgcat tcttaaatgg caaaattgct ggtattgtaa atattacagc tgatcagcgt
     2641 aagagagtcc gtcatattgg agatctcttc attgtgattg gaaaaagata ttggaataat
     2701 ggcttgggaa gtttgttgct agaagaagcg atagagtggg cacaagcaag tggcattctg
     2761 cgtcgtctcc aactgactgt ccaaactcgt aatcaagcag cagtccatct ttatcaaaag
     2821 catggctttg tcattgaagg tagccaagag cgtggggcat atatagaaga agggaaattt
     2881 atcgatgttt acttgatggg taaactgata gattagtaga aatatggtta aaaaaattat
     2941 tggaatggtg ctagctttac tttctgtaac tgtagtagga gtaggtgttt ttgcttatac
     3001 tatttatcaa caagggacag aaaccttagc taaaacctat aaaaaaatcg gcgaagaaac
     3061 caaggttatt gaagcgactg aacctctaac cattctgtta atgggagtgg acaccggaaa
     3121 tgctgaacga actgaaactt gggtcggtag aagtgatagc atgatcttga tgacagtgaa
     3181 tcctaaaacg aaaaaaacaa caatgatgag tttagagcgg gatattctga cgcgcattga
     3241 atcagggaat ggtcaggctc atgaagcgaa actgaactca gcatatgcag atggtggagc
     3301 agagcttgct atagaaacca ttcaaaaaat gatgaatatc catattgatc gctatgtgat
     3361 ggtcaatatg agagggttgc aaaaactagt ggatgcagta ggaggtatta cagtcaataa
     3421 tatcctaggt ttcccaattt ctatcagtga ccaagaagaa tttaatacta tttctatcgg
     3481 tgttggggag caacatattg ggggagaaga agccctagtc tatgcacgaa tgcgttacca
     3541 agatcctgag ggggattatg gtcgtcaaaa acgtcaacgt gaagttattc aaaaagtcat
     3601 ggaaaaagct ctcagtttaa atagcgttgg tcattatcaa gagattctaa aagctttgag
     3661 tgacaatatg cagaccaata ttgatttgtc tgcaaaaagt atccctaact tgctaggcta
     3721 taaagattca tttaaaacca ttgaaactca gcagttgcag ggtgaaggag agatacttca
     3781 aggtgtttct taccagattg tttcgagagc acatatgttg gaaatgcaaa atatactccg
     3841 acgttctttg ggacaagaag aagttactca gcttgaaacc aatgcggttt tatttgaaga
     3901 tttatttggc agagcacctg ttggtgatga agataattaa cttttgatac aaataaaaaa
     3961 atcaatcgta ggaaaattcc tgcgattttt tcaaaaaaat acgaatagat aggtaggagg
     4021 aaacatgaaa gcagaaatca ttgctgttgg aacagagatt ttgacaggac agattgtcaa
     4081 caccaatgcc cagtttttgt cggaaaaact agctgagatt ggggtagatg tatattttca
     4141 gacggctgta ggagacaatg aagttcgtct cttgtctttg cttgagattg ccagtcaacg
     4201 tagcagtctg gtgattttga caggcggttt ggggccaact gaggacgacc taaccaaaca
     4261 aaccctagct aaatttttag ggaaagcatt agtctttgat cctcaggctc aggagaagtt
     4321 ggatatcttt tttgccctgc gaccagacta tgcccgaaca ccaaataacg aaagacaagc
     4381 tcaaattgta gaaggagcga ttccactgcc aaacgaaaca ggactggctg ttggaggaaa
     4441 attagaagta gacggagtga cctatgtcgt ccttccaggt ccgccaagtg aattgaaacc
     4501 catggtctta aaccaacttc tacccaagtt gatgacaggg agcaagctgt attcccgagt
     4561 tcttcgtttc tttgggattg gcgagagcca gttggttacg attttggctg atttaattga
     4621 taatcagatc gatcctacct tggcccctta tgccaagaca ggagaagtca ctctacgtct
     4681 gtcaacaaag gctagcagtc aagaagaggc gaatcaagcg ctggatatct tggaaaatca
     4741 aatcttggac tgccagactt tcgaaggaat ttctttacga gacttttgct atggttatgg
     4801 ggaagaaact agtttagcca gcattgtggt agaagaactg aaaaggcaag ggaaaaccat
     4861 cgcggctgca gagagtttga cggcaggtct tttccaagct accgtggcga atttttctgg
     4921 agtttcaagt atatttgagg gtggttttgt gacctatagc ttggaggaaa aatcaaggat
     4981 gttggatatt cctgccaaga atttggaaga acatggtgtg gtgtctgaat ttacagctca
     5041 gaagatggct gagcaggcac gaagcaagac ccagtctgat tttggaatta gtttgactgg
     5101 agtggcagga ccagatagcc tagaaggaca cccagttggg acagtcttca taggcttggc
     5161 gcaagatcaa ggaactgagg ttatcaaggt taatattgga ggcagaagcc gagcagatgt
     5221 acgtcacatt gcggttatgc atgcctttaa cctagttcgc aaggctttat taagtgacta
     5281 acttttgata taatagtaga taggtctgag gatcattaga atgtaggaga atagaatggc
     5341 gaaaaaacca aaaaaattag aagaaatttc aaaaaaattt ggggcagaac gtgaaaaggc
     5401 cttgaatgac gctcttaaat tgattgagaa agactttggt aaaggatcaa tcatgcgttt
     5461 gggtgaacgt gcggagcaaa aggtgcaagt gatgagctca ggttctttag ctcttgacat
     5521 tgcccttggc tcaggtggtt atcctaaggg acgtatcatc gaaatctatg gcccagagtc
     5581 atctggtaag acaacggttg cccttcatgc agttgcacaa gcgcaaaaag aaggtgggat
     5641 tgctgccttt atcgatgcgg aacatgccct tgatccagct tatgctgcgg cccttggtgt
     5701 caatattgac gaattgctct tgtctcaacc agactcagga gagcaaggtc ttgagattgc
     5761 gggaaaattg attgactcag gtgcagttga tcttgtcgta gtcgactcag ttgctgccct
     5821 tgttcctcgt gcggaaattg atggagatat cggagatagc catgttggtt tgcaggctcg
     5881 tatgatgagc caggccatgc gtaaacttgg cgcctctatc aataaaacca aaacaattgc
     5941 catttttatc aaccaattgc gtgaaaaagt tggagtgatg tttggaaatc cagaaacaac
     6001 accgggcgga cgtgctttga aattctatgc ttcagtccgc ttggatgttc gtggtaatac
     6061 acaaattaag ggaactggtg atcaaaaaga aaccaatgtc ggtaaagaaa ctaagattaa
     6121 ggttgtaaaa aataaggtag ctccaccgtt taaggaagcc gtagttgaaa ttatgtacgg
     6181 agaaggaatt tctaagactg gtgagctttt gaagattgca agcgatttgg atattatcaa
     6241 aaaagcaggg gcttggtatt cttacaaaga tgaaaaaatt gggcaaggtt ctgagaatgc
     6301 taagaaatac ttggcagagc acccagaaat ctttgatgaa attgataagc aagtccgttc
     6361 taaatttggc ttgattgatg gagaagaagt ttcagaacaa gatactgaaa acaaaaaaga
     6421 tgagccaaag aaagaagaag cagtgaatga agaagttacg cttgacttag gcgatgaact
     6481 tgaaatcgaa attgaagaat aagctgttaa agcagtggag aaatccgcta ctttttcgat
     6541 ttttgattca agtttttaga ttatatatag tagcttgaaa taagatatga acaact
//
LOCUS       DAVMEI010000095         6561 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVMEI010000095 DAVMEI010000000
VERSION     DAVMEI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.095"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     regulatory      28..126
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            210..1001
                     /gene="thiD"
                     /locus_tag="VYQ24_001460"
     CDS             210..1001
                     /gene="thiD"
                     /locus_tag="VYQ24_001460"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1968587.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(1211..1843)
                     /locus_tag="VYQ24_001461"
     CDS             complement(1211..1843)
                     /locus_tag="VYQ24_001461"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1968588.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(1836..2639)
                     /locus_tag="VYQ24_001462"
     CDS             complement(1836..2639)
                     /locus_tag="VYQ24_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1968589.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(2639..3163)
                     /gene="thiW"
                     /locus_tag="VYQ24_001463"
     CDS             complement(2639..3163)
                     /gene="thiW"
                     /locus_tag="VYQ24_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1968590.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(3168..3860)
                     /gene="tenA"
                     /locus_tag="VYQ24_001464"
     CDS             complement(3168..3860)
                     /gene="tenA"
                     /locus_tag="VYQ24_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1968591.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(3871..4521)
                     /locus_tag="VYQ24_001465"
     CDS             complement(3871..4521)
                     /locus_tag="VYQ24_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1968592.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(4523..5908)
                     /locus_tag="VYQ24_001466"
     CDS             complement(4523..5908)
                     /locus_tag="VYQ24_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968593.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLNGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(5909..6469)
                     /locus_tag="VYQ24_001467"
     CDS             complement(5909..6469)
                     /locus_tag="VYQ24_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1968594.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRIGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         2029 a         1433 c         1308 g         1791 t
ORIGIN      
        1 aaatatgcta tactaaagaa aaaagaaaac aaccactagg ggtgcgtaaa gctgagatta
       61 acgactgtta gatccctctg actcaatcta ggtaatgcta gctgatggaa gtggaaatga
      121 taatggggac tagcagtctt ctattgcctt tctaaaacag actagcttgt tcttaagaat
      181 acaaacttca gttggttggg aggttttaga tgacttattt acccgttgct ttgaccattg
      241 cagggactga ccctagtggt ggtgctggca ttatggcaga tttaaagtca ttccaagcga
      301 gagatgtcta tggaatggct gttgtaacca gtcttgtcgc tcaaaatacc agaggtgttc
      361 agctaatcga gcacgtttct cctcaaatgt tgaaagccca attggagagt gtcttttctg
      421 atattccacc tcaggctgta aaaactggaa tgttggctac tactgaaatc atggaaatca
      481 tccaacccta tcttaaaaaa ctggattgtc cctatgtcct tgatcctgtt atggttgcta
      541 caagtggaga tgccttgatt gactcaagtg ctagagacta tctcaaaaca aacttactac
      601 ctctagcaac tattattacg ccaaatcttc ctgaagcaga agagattgtt ggtttttcaa
      661 tccatgaccc cgaagacatg cagcgtgctg gtcgcctgat tttaaaagaa tttggtcctc
      721 agtctgtggt tatcaaaggc ggacatctca aaggtggtgc taaagatttc ctctttacca
      781 agaatgaaca atttgtctgg gaaagcccac gaattcaaac ctgtcacacc catggtactg
      841 gatgtacctt tgctgcagtg attactgctg aactagccaa gggcaagagt ctttaccagg
      901 cagttgataa ggccaaggcc tttatcacaa aagctattca agatgcccct caactcggtc
      961 atggttctgg tccagtcaac catacaactt ttaaagatta agaaaaaaaa ctctctagtt
     1021 cccactttaa gggaattaga gagtttttat actcttcgaa aatctcttca aactacgtca
     1081 gcttccatct gcaacttcaa aacactgttt tgagctgact tcgtcagtct tatctaaaac
     1141 ctcaaggcag tactttgagc aacctgcgac tagctttcta gtttactctt tgattttcat
     1201 tgagtattaa ttaggaaaga atgtcatgca actttgttaa aaaggcttgc gtttttgcct
     1261 caatatcttc tgcttgcatc aaatcacgta caacagctac accagctatg ccagtgccca
     1321 taagctgatc aatattctcc gaagtcaagc caccaatagc aactattgga atggcaaccg
     1381 tttggcaaat tgttttcaag gttgaaatca gggtgatggg tgcattttcc ttggtggttg
     1441 tcggaaaaat ggctcctgtc cccaagtaat ctgcacctga tttctccgct tccagagctc
     1501 ttttaaccgt tttagcggtg acaccgagga ttttttcagg acccaagact tgtcgggcaa
     1561 ccgaaactgg gagttcgtca tctccaatat gcagaccagc agcatcaacc gcaagacaaa
     1621 catccaaccg atcatcgatt atcaagggta cctgataagc atctgttatt tccttgactt
     1681 gttttgccag ttgataatat tgattggttg tgagattttt ttctcgcaat tggactatgg
     1741 taacccctga acggcaggcc gtctcaactt ttgcaagaaa gctttccacg gaatcttgat
     1801 agcgattggt taccagatat agtctaagtg cttctctatt cataaacctc tcctttgatg
     1861 gtatctagcc aattttcatc tcttcttagg agcgaaagct gattgagtac ttggtaacga
     1921 aattcttcca atcccattcc ttgaacaact attttctcag cagcgatatt gagataagag
     1981 actgctaagc aagaagcttc aaaaccagtc tttccttggc tgagaaaaac ggctgttaag
     2041 gctccaacca aatctcctgt ccctgttatc cagtctaatt ctgcacagcc atttcccagt
     2101 acagcgacct gatttttcga aacgacgagg tccttgggac ctgtgactaa gaaagacata
     2161 ccatgatagg tctgacacca gtctttcaag acttgaagca aatcctccgt ttcttgatct
     2221 ttagcactcg catcgacccc aacgccgtgg tgctttaatc caacaagact tcgaatttct
     2281 gacatgtttc ctttaaggac cgtaggtcta tagtctaaaa ggtctctgac taagctctta
     2341 cgaatggatg aagctgttac gccaaccgca tctactacca ttgggagaca aacttgagct
     2401 gcataagaag ctgctatacg gattgctttc tccttctcag ctgacaaatg ccccaaattg
     2461 atgaagagag cctgactttg cttagtaaaa tcaagaacct cacggggatc atctgccatg
     2521 acaggtttgc atcccagagc caaaatccca tttgccagca tctcacaaga aatctcattg
     2581 gtcatacagt gaatgaggga actagagcct ataggaaagg gatttgtaaa ttcctgcatc
     2641 agtctatcct ttcactaaag aaatatccct gcactttttt aaagaattcc tgcttgatta
     2701 aaaatcgaaa ggcaataaag gaaatcgctg taccaatcaa ggttgctccg aaaaatcgag
     2761 gcgtgtagat aaaccagcta agcttagcag ctgatcctgt aaagagtacc ataacaggat
     2821 aggaaacaat agaaccaata atacctgttc ccacaatttc tcccaaggca gaaaagtaaa
     2881 attttcgacc gtacttataa aagagacctg ctagaagggc tccaaaagtc gctcctgtga
     2941 gagctaaagg cggaatccct tgagtcgtca tacggataaa ggctgtcact gtagccatag
     3001 ccaaggcata aacaggtccc atcatgattc ccgctagaat attgactaca ctggacatcg
     3061 gtgccattcc ctcaatccga aagataggtg taaggactac atcaagggca atcatcatag
     3121 ataaaatggt caatttgtga acttgtagtt ggtgctttct catgtttcta ttcttctcct
     3181 ttttctaaag actgtaaatc gctcttccat gtctggtgtt ggtaagccat ctcccaaaac
     3241 ttggcttcca tatgaacact gatgtggaag gcatctagca ttttttgctt atctgtctca
     3301 tcactttctc gatagagctg attgaccagt gctccctcct ctctgatctg ttgctctaac
     3361 tcatccgtaa tataagtttc aatccattgt tgatagagag gatttggtga tggtttaaga
     3421 ttaagtgatt tgcctatatc atggtataac caaggacaag gaagcaagct tgcaaaagcg
     3481 atggctaagt tcggttctgc aaattgccta taaatatgag aaatgtaatg ataacaggtt
     3541 ggagcgattg gatgttgctc catttcctgg tcgctgattt ccaattcctt gaaaaattgt
     3601 tggcgaataa ataactcacc ctccactaaa ccctgagcat tttgtttcaa gagtcttttc
     3661 atctcttggt ttgaagtctt atcagccaaa agatgataga tttctgagaa agccttcaga
     3721 tagtaggcat cctgaatcag gtaatagcgg aaaatggcag gttctaaatt cccctcttgt
     3781 aattgtaaaa taaagggatg atgaaaggaa gcctgccaag ctttcttgga taattccatc
     3841 gcaatatctg taaattccat aataactcct ttataaaaat agactggttt gaagcaataa
     3901 aaagaaaagc aggtagatta attttgtttt tttaggaata taaaaagtcc gatagctatt
     3961 cttcaactgt gcatgttcgt catatccgtg agcagataga gctctcaggt aaagatggcg
     4021 ccacctaaag actgtcatca gaaccttact gtaaatcaag ggcgaccaaa aatgtagttc
     4081 ttgaccacgt aataggcaag cttctttgag ggacttgatt tcttgctgaa tgagaggaaa
     4141 agaattgaat accacaatca aggcatagga ccaagagcgt gatagcccct tttgagccaa
     4201 gtacaagaga agctctttta gtgaaataga ggaaacaaag acaaggccga tacaaactgt
     4261 cacaaaggcc ctcgttccaa gcatgactgc ctgtgaagca tctccgtgta actgaactgc
     4321 ccagtagttg gcaaaagatg gtaaaatggc aagtatgatc atccaagcta acattttaaa
     4381 tcgacggtaa tagagcataa agagaataca aaatgcgact accgaaagat tcagagcaat
     4441 cgaaggaatg aaagatgttt ccaaggataa aatcagcaag aagagactga taatcggtgt
     4501 ctgggttgct actttgacca tactatctca cctccccttg ggtattgcta ctctgagatg
     4561 taagtggttt ggtaatggtc acttctttca catgccgaag accctgacta gtcatctcaa
     4621 tccaataatc aaccacagaa atcaaagggt ctaaacgatg actaatgagc agaaaacttc
     4681 ttccttgatt cctctcctcc acaatccact tgcaaaaata atggcaggct ctatcatcca
     4741 aacctgcaaa aggttcatct agcaagatca cggaagcctt actggtcaag atggtcagga
     4801 gctgaagaat tttttgctga ccaccattta attgataggg actcttatcg actgcctgct
     4861 ccaaatcaaa atatcgtaaa gcttgaaaaa tccgctgatt tctttcagaa tcaggtccat
     4921 ctaattgaag ctcctctcgc agactgactc ggataaactg cttctcagct tcctgaacaa
     4981 caccagtcag atcacgatac aaactctttt tctttttcag gaccgaaccc ttccatgcaa
     5041 tgcgcccctt atacttttga aattgaagaa tagaccgaaa gagggttgat ttcccgacac
     5101 cattgtcacc caggatacag gaaatccctt gatagaatgt gaaatcagca attgaaaaga
     5161 gggggcgatt tccaaactca caagtcgttc tatccatatg gaatagttcc gggctagaag
     5221 caacttcctt tgaagcaacc tgtgtcatct cataggaagg gatttgaaac acttccctta
     5281 gttttccgtc tcttagctca accatatggt cgatataggc tttatagtca gataaatcat
     5341 ggtcgcacaa aataactgtc tttccatcaa gagccaactc ttttagaatc tccaatatct
     5401 cgattctgct cttgcggtca atggaagcaa aaggttcatc caagagatag accctaggat
     5461 tcatggcaaa gaggacagcc agcgctgctt tttgcttttc cccacctgat aggtgatgaa
     5521 tgggacggtg taagattgcc tcgcaacgac attgctggac aacctctgct attttagaat
     5581 caatttcctg aaggtgatag ccgatattct ccaaggtgaa aatcagctcc tcaaacaagc
     5641 tctccatggt aaattgatga ttgggatttt gaaagagaat gccaaccttc tggacacgtg
     5701 cgatgataga aagttgactg acctcgctcc catctatcag gacttgacca ctatagggaa
     5761 gagaactgac ttgggcaatc atttgaaaga ggctggattt tccagaccca ctactcccaa
     5821 ctaacaaggt aaaggcttgc gcatgaaaag taaaatcaaa cggctcagag aagattgggg
     5881 actgaatcgc tcgtagttcc agacccatct atgcttttcc tccagttgca aactgatgat
     5941 agagtttgac aatggcacga accaagatgg tacagaagaa ataaacagaa ataaaacgta
     6001 ccacaagtaa ggaaaggaca aacggaaggg aaaaggcgta gtaacctaac ttaatgtatt
     6061 catagacaaa gctaacaagc gtaatcccaa tactattagc agttagagag agccaacttt
     6121 catagcgatt cttagttacg ataaaaccaa attcacttcc caaaccttga actaagccag
     6181 acaaaagagc tcctagacca aattggctac cataaaggac ttcagcaagc gcagctagca
     6241 cttctccaat cgttgcactt ccgattctcg gaacaaagat ggcagcaatg ggcgcagcca
     6301 tacaccagag accgaagagg atttcattgg caaaggcctg caaaccaaga ggtgttaaga
     6361 gtagactgag aatattatac acatatcctg aaccaacgaa aaccccacca aaaaagatag
     6421 acaagaaagc aagcaagata acatctttta actgccattt tttcaacata aaaaactcct
     6481 tttttaaaag aaaagtgggg cactcaagaa gaccgaccta aatactttgt atagcagatt
     6541 gaatttagaa cagtacacag g
//
LOCUS       DAVMEI010000096         6549 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVMEI010000096 DAVMEI010000000
VERSION     DAVMEI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.096"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(53..1057)
                     /locus_tag="VYQ24_001468"
     CDS             complement(53..1057)
                     /locus_tag="VYQ24_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1968595.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(1170..1387)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1470..4166)
                     /gene="ppc"
                     /locus_tag="VYQ24_001469"
     CDS             complement(1470..4166)
                     /gene="ppc"
                     /locus_tag="VYQ24_001469"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1968596.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(4186..5415)
                     /locus_tag="VYQ24_001470"
     CDS             complement(4186..5415)
                     /locus_tag="VYQ24_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1968597.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFAGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(5543..5796)
                     /locus_tag="VYQ24_001471"
                     /pseudo
     CDS             complement(5543..5796)
                     /locus_tag="VYQ24_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(6313..6534)
                     /locus_tag="VYQ24_001472"
     CDS             complement(6313..6534)
                     /locus_tag="VYQ24_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968598.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
BASE COUNT         1976 a         1367 c         1124 g         2082 t
ORIGIN      
        1 tctatattca tctttttaaa aaatgggaag agtctcctcc tccctacctt atttattcga
       61 tgacttgtcc tgcttctttg agaacagact caggaatagt aatacctagt tcttgtgcta
      121 tttttttatt gatgactgac ttaccagttg aaaagacatt gactggggta tcggctggtt
      181 ttgcaccttt caagacttgc acaatcattt tacctgttgc cacaccaagg tcatgttggt
      241 caattacaac tgatgccaaa ccacctactt ctaccatagc tgtcgcactg ggataaattg
      301 gtttcttaga actttgattg ctagagacaa ccgttggaaa tcctgatgca atggtgttat
      361 caattggaac ccaaatagca tctaccttgc tagtcataac agtgacagtt gaggcaattt
      421 catttgttga aggaactgca aatgtttcca ctgtcagacc tgctttttca gcataagcct
      481 taaattcttc gacctgtgtt tttgaattgt cttcgctact tgagtaaaga gctccgattg
      541 ttttcacatt cggtgtcaga gccttgatga gttcaacttg ttgttgagct ggattgtggt
      601 cagatacccc tgtaatgttg ccacctggtt ttttcaaatc tttaaccaag ttagcaccaa
      661 ttgggtctgt aatagcggcc atgataaccg gtaggtcttt tgttgcacta gccaaccctt
      721 gggctgctgg tgttgcgata ccaaccacaa ggtcattccc atttgcaacc aattgtttac
      781 tcattgtcgc aaccttactt tggtcacctt ctgagttcat aaaatcaatt ttaacttgat
      841 catctttata tccttcttct gcaagtccat cttggatccc tttataaatc aaatcaaggg
      901 atggatggct cacaaattga agcacaccaa tcttagttat tttcttctca tccttattat
      961 tctgagcttc tgatttattc attgaagaat aaatcaagct tcctgctact aagactgcta
     1021 atgcagcaat aattccaatt aaacgtttat ttttcattct gtttctcctt tttatttcct
     1081 ttaacatatt tcacttatct aaacaagcga cgccatcgtt ttctttccat actaacctcc
     1141 atcaaaaaaa tcctcacaca aaaaacttgt gtgaggacgt cgatgcgcgg taccacctca
     1201 attataggga atttccctat cgctcagtct cgcaataacg agatgcactg taaggtgtgc
     1261 ttaccgaatt tttataattt caaattctaa atagcattca gcccaatttt catcatcgcc
     1321 acttatctgt tttcagctac cacagactct ctaaaaagtt tgtatgatta cttttctgaa
     1381 tggttaaatt atatcatttt taaactactt gtcaatagtc tattagaatt tttttgatat
     1441 tcacttttag cattcactct tgaaaattat caacctgaat tacgcaatcc tgtcgcaatt
     1501 ccgttgatgg tgatatgaat caatcgttct tgatcactgg acaattctcc acgacgttgg
     1561 cgtttaatca actctaactg aatatagttg agaatattaa agtaaggcat acggtaatcc
     1621 agactagctt ttagatatgg attgtcagct aagagttcgt catgtccttc aatagccaag
     1681 ataacgttct tagtaacttg ccattcattt aaaatagtct catagatggc cttaacttgc
     1741 tcgtcttcac aaagtttagc atattcaaaa gcaatattca tatttgattt tgacaaaacc
     1801 atatcaacat ttgaaagaag cgattggaag aaaggccaat tttggtacat atctcgtaag
     1861 atagcaatat tctctggatt tttattgata aattccttga agcttgaacc aaccccgtac
     1921 catccaggga acataacacg actctgtgac catgagaata cccaagggat ggcacgcaaa
     1981 ccaccgattt cagtaatagt cttacgagcg gctggacgag aaccaatatt aaaacttgaa
     2041 atagccttga ttggacttga ctcgaagaaa taatcataga aatgctcatt accaaagacc
     2101 aaatcacggt agatatcgta actacggtcc actacttgat ccataatggc ttcataacga
     2161 tttggggtat tggtatcgct cttcttctga gtaatcatac ggttaatagc tgccgatact
     2221 agcatttcaa ggttatagta agcggcgtct ttgttaccgt atttattccc aattacttca
     2281 ccctgctccg tcaagcggat acgatccttg atagacttga gcggttgaga tgtaatggct
     2341 tcataggttg gcccaccacc acgaccgaca gtaccaccac gaccatggaa gaaggtaacc
     2401 ttaacgccaa attcatctcc aatagcagtc aattgttgtt gagccttgta gagggtccaa
     2461 catgatgaca agtaaccgcc atctttatta ctgtcagagt agccaagcat gatttcttgg
     2521 tagttatttc gtgagtcaat ccattttttg gcaaggctaa gagaaagata ttttctcatt
     2581 gtttcttctg aatgatccaa gtcttcaatt gtttcaaaaa ggggaacaat ctgaacacgc
     2641 gccctttccg tatccaccag tcctacttct tttaacagaa tagctaattc tagcatatca
     2701 gaaaggctgg ttgcatgtga aatgatggtc tgacggatga catcatctcc caacttatct
     2761 ttcaaaacac gagccgtctt aaaaatagct aattcttttg ctaataattc tgatttttct
     2821 gcgtgagtcg cagaaagaat tcggggatct tcttctaatt ctttcaagag aaggtcacac
     2881 ttttcttctt cgctcaactc gctataacga gaatgaattc ctgctgattt caagagttct
     2941 gccacacagg cttcatagac gctagagtct tgtcgcatat caattgatgc taggtaaaaa
     3001 ccaaatatct ctactgcctg caataattcc acaaaatcac ctgaaatcaa ggactcaccc
     3061 ttattttcta gtagagaatc tcgaatggca atcaaatcct tgtagaaatc attggcggtt
     3121 tcataacgag tcccaacttc ttcatcctca atcagatagg ttttagttgc ctgaattttt
     3181 gattgaatat caaacaaggc acgacggtaa agctcttttt cgcggtaaat cgagttatcc
     3241 ttggattgac gagccatttc tctgacttgc ttgctgacgt tgacaatgct agttgaaaga
     3301 gaaaattcac gataaagttg gtaaatcttt ttatcatagt agttcatgat gacttcacat
     3361 tgagtgagtg cagactgctt caaggtctct gctgtaacaa atggatttcc atcacggtca
     3421 ccacctatcc acatacccat ggtgattggt ttagcctgtt ttaaattcag accatgcgct
     3481 tgcgctaagc gcttatactc cgtcgtcaaa tgaggtacag ctttcaaaaa ggagctgtta
     3541 taatattcca tagcattcgt gatttcgtta gtcactttta attttttctc acgaatcatg
     3601 tctgtctgca tgataatttc gatgtaacga cgcaaatcat tgtaccattt atctttattg
     3661 atcaacccca acttaacatc acggtattta cgcaaaagac tatgaatatg atttgttaaa
     3721 tccaacatac ttttgcgttg cacttgtgtt ggatgggctg tcaaaacagg gacaacattc
     3781 aagtgttcta ggatctcaac ggcattttcc ttttctgcta ccaatttaat cgttgtagat
     3841 aatttaccta aatagtcctg atcaatatta ttttgatgat tgatttcata agctaaatcc
     3901 acatcctctg aaatattgat caaaagaggt aagatagaga aatagcgtga aatatagacc
     3961 atttcatcat ttgataagct agtcactaga cggtttagac cttgataatc ttcctgcgtt
     4021 gataattctt tcaactgtat tattttttca aaggtctctg gggcaagcat atttttagta
     4081 atatcttcca gtaattctgt tagaatcaag acttcttctt gcacaacact tttattacta
     4141 taattttcta atttttgaag agacataaac tatcctttct ttatcctact tcaacagaag
     4201 gttcattggt tgattttcca attctcggta caacttagcg cgtttttcac tggcatcaat
     4261 atttaagaca aaggctactg ccactgataa gactagaaga ctatttccac cctgggataa
     4321 gaaggggaag gttactcctg tagatggaat caagcccgaa atccctccga tattgacaaa
     4381 tacctgaacc aacatcatcc ctccgacacc gagtgcaacc atggcattga aaggattctc
     4441 cgctcgaata ccgaccaaga taatccgcaa aatcatgaaa aacaagagag ctaaaataag
     4501 actggcacca gcaaagccaa attcttcaat cacgatagaa aagacaaagt ctgtatgagc
     4561 ttctggcaaa taacctcgtt tttcaatcga gtttccaaga cctagaccaa accaaccacc
     4621 attgaccatg gcaaaataag aattagctaa ctggtgacct gcatcagcac gatcggcaaa
     4681 aggattaaaa aaggcactaa agcgcttggc tacatagcca aatactggaa tttttgaaaa
     4741 ggtctcaaca ccgattaggc tgatagtggt caagacaaag acagaagcgg cagatacgag
     4801 cgccagaatg gttgaaaacc agcgataagc gattccacta actgtataca taatcaagga
     4861 aaccaagact aaaatagtcg catttcctaa atcagggaaa attcccaaac ttccaatcag
     4921 aactaggaga acgaatcgcc aatcattaaa agcacgggga agccattgat tttgagtcaa
     4981 aacttgaaaa tcataagtag ctatttcttc ttgctgtttg gagaatcggt gagctaaata
     5041 ccaaataata atgattttta agtactcagc tggctgaata gttactcctg caaccgaaat
     5101 ccaaccgtat gccccgttta cggaaatacc aataaaacga gccaagaaca ataaaagcat
     5161 ttctattaat ataactaaaa tgattagtcg ctcatttctc aaaaaatcta gtctcaattt
     5221 ataaattaag gcaatcagta tcaaactaac aatccaaaag attccttggt ttcgaaccaa
     5281 ctgcaaggcg ctcttgcctt cttcaattaa aatagcactg gtggtcgaat agaccacaat
     5341 caagcccaaa atagataaaa gcaagtaggg aatcaagatg gaataattta ataagtgcct
     5401 cttactaatc ttcatagcat caccactcta ctaggataca gacaaatctg ttccaaattc
     5461 cgttttattt tctagttttg attgctatta taccattttt tcagaaagaa aaaaactctt
     5521 accttacaat ccttcgaatt ttactgtttt tcataattag ggcagaattt gaaatcgtaa
     5581 aaccattttg aacaagagtt acttcaaccc attggctccg acggattaag ttgctttcgt
     5641 gaatgccaaa atcagccgca atttgttcat aagtgcgcta ttctcgcatg tattgaatag
     5701 ttaccatgag gagatcgtct aggcttaatt tggtttttcg tccacctttt gcgcgtttac
     5761 gttgcgttga taagctgttt ttaacacagc taacatctcg tgcgctgaac acctacaagg
     5821 ctcttgaaac ggctatcgct taattgttta cttgcttcat catccatagt tttattgtat
     5881 tgtatttttg tttcgcagga agtctattat tcctttcgtt ccaaggtttt tacgctttta
     5941 gcaacaagct tacacactcc acgtgatgcg tctgtggaac tcaaaaggtt cggggaacct
     6001 tttgaggatt aattgcgttt ctctaacaaa ctcacacatt taacgtggca tgactgatga
     6061 agtcatcata agaatttcac ttgctgctct tttacgatgg cagcctgaac ggtcagaagt
     6121 atcattgtat atcatttgta tcatggcata taaagtatcg ctctatttta ttgatggttt
     6181 taaaatcgct cttatcatgg cgagccattc aaatttgctc cacaaatgag gaaagtacca
     6241 ttttggacta tttaattttc aaagaacaaa taaaaaatct cctcattttc acactctatg
     6301 aaattgagga gattattttc taaagcgaat tttatcagga aaatctgtca attccaatag
     6361 gtagtctgta ctgacatcgt aaaagtttga gagagttacc aatacatctg ctgttaacgt
     6421 atgctcaccc cgttcaattt tagcataagc tgagtttgta aacgaaagta ttttagctat
     6481 ttgtttttgg gtcaaatcgt gatcttccct caaatctctc aaacgtctgt acatctagct
     6541 ttctcctga
//
LOCUS       DAVMEI010000097         6505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVMEI010000097 DAVMEI010000000
VERSION     DAVMEI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.097"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            169..1119
                     /locus_tag="VYQ24_001473"
     CDS             169..1119
                     /locus_tag="VYQ24_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1968599.1"
                     /translation="MGKKRWARNGSESNDASYAQVVSLYDDTSISVSNNETDKVLTGS
                     LYTETNEQGLTIPSSLLKNWNEQTGKNLTTNDLIGKSVSASIVESAAETSKIAQFQTK
                     IVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQK
                     NKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIG
                     VMKAVGYQNRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFE
                     LNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1296..2303
                     /gene="gap"
                     /locus_tag="VYQ24_001474"
     CDS             1296..2303
                     /gene="gap"
                     /locus_tag="VYQ24_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1968600.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2371..3207)
                     /locus_tag="VYQ24_001475"
     CDS             complement(2371..3207)
                     /locus_tag="VYQ24_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1968601.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVRWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGKYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3333..5528
                     /locus_tag="VYQ24_001476"
     CDS             3333..5528
                     /locus_tag="VYQ24_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1968602.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQSLAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5581..5733
                     /gene="rpmG"
                     /locus_tag="VYQ24_001477"
     CDS             5581..5733
                     /gene="rpmG"
                     /locus_tag="VYQ24_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1968603.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5743..5919
                     /gene="secE"
                     /locus_tag="VYQ24_001478"
     CDS             5743..5919
                     /gene="secE"
                     /locus_tag="VYQ24_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1968604.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5974..>6505
                     /gene="nusG"
                     /locus_tag="VYQ24_001479"
     CDS             5974..>6505
                     /gene="nusG"
                     /locus_tag="VYQ24_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1968605.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2025 a         1133 c         1410 g         1937 t
ORIGIN      
        1 atatcaaaaa gcacctcaaa aaggtattac caagttgcaa taccttttat gaggctcttt
       61 tgtcttgttc ttgtttcaat tgactatagt tcacggatac agttgggaaa gaagctatta
      121 aatatttgga gagtcctttt gggatgaaca tgtcaaatat tactatagat gggcaagaaa
      181 agatgggcaa gaaatggatc tgagtcaaac gatgcttctt atgctcaagt agtgagtctt
      241 tatgatgata caagtatctc tgttagtaat aatgagacgg acaaagtgtt gactggatcc
      301 ctctatactg aaacaaatga acagggatta acgattccaa gcagtttact aaaaaattgg
      361 aatgaacaga caggaaaaaa tttgacaact aatgatctta ttggcaaatc agtctcagcc
      421 agcattgtag aaagtgctgc cgaaactagt aagattgctc aatttcaaac gaagattgta
      481 cgtgtaatca atgatgaaga tgacatggag gacagcaaca gtttcatgct gtctcatcaa
      541 atggaaacga ttttgaaaga ggctggattt acgaaagctg tatcttattt tatcttggaa
      601 ctcaaagatc catcacagac aaaagtagta acagaagaat tacagaaaaa taagaagtat
      661 actgtgcttt ctcaacagag agttcttgat attgtgatta cctttattcg tgttattcag
      721 ggattattga ttgtgctttc atcacaagct attgtggtag cggcggttat gattggtatc
      781 attatttaca tcaatatcat gcaacgttcc aaggaaatag gtgtcatgaa agcagttggt
      841 tatcagaatc gtggtgtcaa aggaattttt atttacgagg ctatctggat tgtaggcatc
      901 gccttgctgc tggcattttt ggttgcacaa ggggtgggaa gtttggcgaa tgcgattgta
      961 agtcactttt acccatccat cactaaggtt tttgaattaa atcttttatc tgttttagga
     1021 actctagttt tcgctctatt acttggttat gtctcagcct acttcccggc gcgtaagatt
     1081 agtaaaatgg atcctgtaga atcgttacgc tatgaataat aagggattcc ttgatttaca
     1141 gtcttaaaaa gaaactcaca tagaaaaaca atggccatac ttgcaattca actcaaatag
     1201 ttgtataata ggtttgttga aaggtgattt gtagtgattc aagttactct tttcacaata
     1261 aaaatttcat gattttcata aggaggaaat cactaatggt agttaaagtt ggtattaacg
     1321 gtttcggacg tatcggtcgt cttgctttcc gtcgtatcca aaacgtagaa ggtgttgaag
     1381 ttacacgcat caacgacctt acagatccag ttatgcttgc acacttgttg aaatacgaca
     1441 caactcaagg tcgtttcgac ggtactgttg aagttaaaga aggtggattc gaagttaacg
     1501 gtaaattcgt taaagtttct gctgaacgtg atccagagca aatcgactgg gctactgacg
     1561 gtgtagaaat cgttcttgaa gctactggtt tctttgctaa gaaagaagca gctgaaaaac
     1621 accttaaagg tggagctaaa aaagttgtta tcactgctcc tggtggaaac gacgttaaaa
     1681 cagttgtatt caacactaac cacgacgttc ttgacggtac tgaaacagtt atctcaggtg
     1741 cttcatgtac tacaaactgc ttggctccaa tggctaaagc tcttcaagat aactttggtg
     1801 ttgttgaagg attgatgact actatccacg cttacactgg tgaccaaatg atccttgacg
     1861 gaccacaccg tggtggtgac cttcgccgtg ctcgcgctgg tgctgcaaac atcgttccta
     1921 actcaactgg tgctgcaaaa gctatcggtc ttgtaatccc agaattgaat ggtaaacttg
     1981 atggatctgc acaacgcgtt ccaactccaa ctggatcagt tactgaattg gtagcagttc
     2041 ttgaaaagaa cgttactgtt gatgaagtga acgcagctat gaaagcagct tcaaacgaat
     2101 catacggtta cacagaagat ccaatcgtat cttcagatat cgtaggtatg tcttacggtt
     2161 cattgtttga cgcaactcaa actaaagttc ttgacgttga cggtaaacaa ttggttaaag
     2221 ttgtatcatg gtacgacaac gaaatgtcat acactgcaca acttgttcgt actcttgaat
     2281 acttcgcaaa gattgctaaa taattcttga gttgatagaa agcaaggctt tgtggtcttg
     2341 cttttttata tggaaaaatg gatgacacga tcatccattc ttttttaatt ctttttcaaa
     2401 tgtatttgaa agggtagtga aagttagcct ctctaaagta agtgggtggg taaaggaaag
     2461 tcggaaggca tgaagcataa gccggcttgt ctttgattta ctattataga gagggtctcc
     2521 caggatagga agattatgat gcaaaaggtg cacacgaatc tgatgggttc gccctgtctt
     2581 tagcttgcaa tgagccaagg aagtcttgtt tgagaattgc tttaatctgc ttacatgcgt
     2641 ttcagcatat ttcccatttt ttgcatcaac tattcttttt ctacgatcat ggcgatcacg
     2701 tccaattttg tctctgaaaa caagttcttt tctgttgata tttccatcaa ctagagccca
     2761 atattctcta gaaatctctt ttttctccaa taagcgattg agaatgggca ggataaaagg
     2821 atttttggca aagagaacta agccactggt ttccatgtcc agacgatgaa cgacatagca
     2881 ggtttggcca acataggtac tgacatggtt aagaagggca atttcgtttg gttgattacc
     2941 atgcgttttc atcccctctg gtttgtttac aataatcaag tgttgatctt gataaacttc
     3001 ctgcactaag tctgggttgc cccaagggat cgtcttttgg ggataatctt cctcgtcaaa
     3061 agtcaactgg caaacatctc caggatttac gatttcgttc cagcggactt cttcttgatt
     3121 tatcaaaata tgtttcttga ttctcaaaaa atgacggatt tttctaggga tgaggagttg
     3181 ttcctcaagt aattgcttta ccgtcatttg aggtagagag gcgggtaatg taaatgtgaa
     3241 ttgcatacag atattgtaac aaaaaaagcc ctatttggat aggaaatagc taaattcttg
     3301 tcttcctatg atgaagatga taaaataaac gcatgaaatt agataaatta tttgagaaat
     3361 ttctttctct ttttaaaaaa gaaacaagtg aactagagga ctctgattct actatcttac
     3421 gtcgctctcg tagtgatcga aaaaaattag cccaagtagg tccgattcga aaattctggc
     3481 gtcgttatca tctaacaaag attatcctta tactaggttt gagtgcaggc ttgctagttg
     3541 gaatctattt gtttgctgta gccaagtcga ccaatgtcaa tgatttgcaa aatgccttga
     3601 aaactcggac tcttattttt gaccgtgaag aaaaagaggc tggtgccttg tctggtcaaa
     3661 agggaaccta tgttgagctg actgacatca gtaaaaactt gcagaatgct gttattgcga
     3721 cagaagaccg ttctttctat aaaaatgacg ggattaacta tggccgtttc ttcttggcta
     3781 ttgtcactgc tggacgttca ggtggtggct ctaccattac ccaacagctg gctaaaaacg
     3841 cctatttatc gcaggatcaa actgttgaga gaaaagcgaa agaatttttc cttgccttag
     3901 aattaagcaa aaaatatagt aaggagcaaa ttctaaccat gtaccttaac aacgcttatt
     3961 ttggaaatgg tgtgtggggt gtagaagatg cgagtaagaa atactttgga gtttctgcat
     4021 cagaagtgag tctggatcaa gctgcgactc tggcagggat gctcaagggg ccggaactgt
     4081 ataatccctt gaattccgta gaagattcta ctaatcggcg cgatactgtc ttgcagaata
     4141 tggttgcagc aggatatatt gataaaaacc aagaaaccga agctgctgaa gttgatatga
     4201 cttcgcaatt gcacgataag tatgaaggaa aaatctcaga ttaccgttac ccctcttatt
     4261 ttgatgcggt ggttaatgaa gctgtttcca agtataatct aacagaggaa gagattgtca
     4321 ataatggcta ccgcatttac acagagctgg accaaaacta ccaagcaaat atgcagattg
     4381 tttatgaaaa cacatcgcta tttccgaggg cagaggatgg aacgtttgct caatcaggaa
     4441 gtgtagctct cgaaccgaaa acagggggag ttcgtggagt tgtcggtcaa gttgctgaca
     4501 atgataaaac tggattccgg aatttcaact atgcaaccca atcaaagcgt agtcctggtt
     4561 ctacaattaa gcctttagtt gtttatacac cagcagttga agcaggctgg gctttgaata
     4621 agcagttgga taaccatacc atgcagtatg atagctataa ggttgataac tatgcaggga
     4681 tcaaaacaag tcgagaagtt cctatgtatc aatccttggc agaatcgctt aatctacctg
     4741 ctgttgccac tgttaatgat ttgggtgttg acaaggcttt tgaggcaggc gaaaaattcg
     4801 gactcaacat ggaaaaggtc gaccgtgttc ttggtgtcgc cttgggaagc ggtgttgaaa
     4861 ccaaccctct tcaaatggct caagcatacg ctgcctttgc aaatgaaggt ttaatgcctg
     4921 aagctcattt tattagtaga attgaaaatg ctagtggaca agttattgcg agtcataaaa
     4981 attcacaaaa acgggtgatt gataagtctg tagctgacaa gatgaccagt atgatgttgg
     5041 ggactttcac caacggtacc ggtattagtt catcgcctgc agactatgtc atggcaggga
     5101 aaactggaac aactgaagca gttttcaatc cggagtacac aagtgaccag tgggtaattg
     5161 gttatactcc ggatgtagtg attagccact ggcttggctt tccgaccact gatgaaaatc
     5221 actatctagc tggctctact tcaaacggtg cagctcatgt ctttagaaac attgccaata
     5281 ccattttacc ttatacgcca ggaagtacct ttacggttga aaatgcttat aagcaaaatg
     5341 gaattgcacc agccaataca aaaagacaag tacaaaccaa tgataatagc cagacagatg
     5401 ataatttgtc tgatattcga gggcgtgcgc aaagtctagt agatgaggct agccgggcta
     5461 tctcagatgc gaagattaag gaaaaggctc aaacaatatg ggattcgata gtcaatctat
     5521 ttcgctaaga tgcttgtcaa agcctagctt tcttgttata atggataaga tggaggcgtt
     5581 atggcactaa aaaaagcaag cctagcttgt gcggtttgtg gttcgagaaa ctattcaatc
     5641 aagatcagcg gaaaccccaa gcctacacga ctagaagtaa ataaattttg taagcattgt
     5701 ggcaagtaca ctacacacag agaaacgaga taggagagag cgatgcgttt tattggagat
     5761 atttttagac ttcttaaaga cacaacatgg ccaactcgca aggaaagctg gagagatttt
     5821 cgttctatca tggaatacac agctttcttt gtagtaatta tttacatttt tgaccagttg
     5881 attgtttcag gtttgattcg atttattaac attttttaga agattagtgg agttaattac
     5941 actagaaatc ttctatttat gaaaggaaat atcatggata gttttgataa aggatggttt
     6001 gttttacaaa cttattctgg ttatgaaaat aaggtgaaag aaaatctatt acaacgtgca
     6061 caaacctaca atatgttgga taatattcta cgcgttgaaa ttccaacaca aacagtgcaa
     6121 gttgaaaaaa atggaaagag aaaagaagta gaagaaaatc gctttccagg ttatgttctt
     6181 gtagaaatgg tcatgacaga tgaagcttgg tttgttgttc gaaacacacc aaatgttaca
     6241 ggatttgtcg gatcacacgg gaacagatca aaaccaactc cattattgga acaagaaatt
     6301 cgtgacattt tggtatctat gggacaaact gttcaagaat ttgatttcga tgttgagatt
     6361 ggtcaaactg tacgtattat tgatggtgct tttgcagact acactggtaa gattacagaa
     6421 attgataata acaaagtgaa aatgattatc tctatgtttg gtaatgacac agttgcagaa
     6481 gtaaacctaa accaaatcgc agaat
//
LOCUS       DAVMEI010000098         6349 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVMEI010000098 DAVMEI010000000
VERSION     DAVMEI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.098"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            212..448
                     /locus_tag="VYQ24_001480"
     CDS             212..448
                     /locus_tag="VYQ24_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968606.1"
                     /translation="MNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKL
                     PKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            432..728
                     /locus_tag="VYQ24_001481"
     CDS             432..728
                     /locus_tag="VYQ24_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968607.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLLEDNKID"
     gene            950..1609
                     /locus_tag="VYQ24_001482"
     CDS             950..1609
                     /locus_tag="VYQ24_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1968608.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1634..2701
                     /locus_tag="VYQ24_001483"
     CDS             1634..2701
                     /locus_tag="VYQ24_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1968609.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDAKEMPKDWT
                     ELASKYKGQYSIYGLTGGTGRAIYASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESTVVKVLDKENAIQYGMIWGSGALVGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            2804..3814
                     /locus_tag="VYQ24_001484"
     CDS             2804..3814
                     /locus_tag="VYQ24_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968610.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINILTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3826..5517
                     /locus_tag="VYQ24_001485"
     CDS             3826..5517
                     /locus_tag="VYQ24_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1968611.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5520..6230
                     /locus_tag="VYQ24_001486"
     CDS             5520..6230
                     /locus_tag="VYQ24_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1968612.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2168 a          870 c         1217 g         2094 t
ORIGIN      
        1 taatgcaaat gggaaagaac ctgttatacg tgatagcgat ggtaatttca atattgtaac
       61 ggagaatggg ctttacaaaa ttgtaacaaa taatttaagt gataaaaacg aaaaagaaaa
      121 aaacaaagag gaaaaacaat ctaattcaaa taatgtaatt gatagtaacc agaagaacgg
      181 agagtttaac tcaagtaaag ataatagaca aatgaatgac aagatcgaca ataaacaaga
      241 taataagaca gaagaagtaa actataaaat agttggagat ggcagagaaa ctgaaaatca
      301 tattaataaa tctaaagaaa tagtagatgt aaaacaaaaa ttaccaaaga ctggttcgaa
      361 caagattatg gaactattct taacagtgac aggaattggt ttacttttga cactaaaagg
      421 gttgaagtat tatggtaaag ataaataaaa tttgttcgat acaaggaagt tccgtagaaa
      481 atgaggatat tgtaggttca caaaatcagt atttttggat tattggtgga gctacagatt
      541 tatataattc taaagaggag ataggttatt cagtctcaga agttgtacat attttatcag
      601 aaagtttgtc ggttaattgt aaagaatcaa aaactcttaa acaaatattt gaaactgctt
      661 tactcgaggt taaagatgaa atcggtttaa actcatataa attattggag gacaacaaaa
      721 tcgattgaag acatgtgtag agatacatgg aattggcaat caaaaaatcc caatggctat
      781 aattgatgat actgtctata tgagtgctgt attttggaaa aattacttaa ccttttaatg
      841 aaagataaat taaaaaaaat gtttgacagc gcttgacaaa ggaaacggtt tcattgtata
      901 atgtaaacac gatgacaccg atgtcaaata taaaaaagaa ggtctttata tgaaaaactt
      961 aacaaaaatt aagttcaaag agaatgggga atttaatcat ttccctggga atacagttgt
     1021 agcaaatctt tatactaaac aagatttgat ggaagttgtt gatattattc aatcacgtta
     1081 tagagaatta ccatttatcg ataagtttac tttaactcca aggaattcta ttcacatgac
     1141 tgtaattgaa ttgttgtgcc atgaaaatcg tgaaacggag ttttggagca gtaatcttcc
     1201 tctagataca cctttacagg aaatacatga ttactttgct aaacaacttg aaatttttcc
     1261 attgttggat gaagaaattc atatgcgtat aactgaaatg ggaaaacaaa acatactagt
     1321 tgaacctgca gatgaagctt ctgcaaagag attagaagaa attcgtactt atgtctcaga
     1381 aaaagcaggt gtttgtttcc ctaatcatga tagatatcaa ttccatattt caattgggta
     1441 tcttcggatt cctctaaccg aagaggagga agaagagttt actaaagtca gagcagaatt
     1501 aactgaaatt ttattagaga agattccaac tattactgta aaccgtattg actatactgt
     1561 atttgaagat atgagacaat ttgttccata tcacgaaaaa tttaaataaa ataataaaaa
     1621 aggagaactt atcatgaaaa aaatgaaagt ttggtctact gtacttgcaa cgggagttgc
     1681 tcttactaca cttgctgctt gctctggagg ttcaaattct acgactgctt cttcatctga
     1741 agaaaaagct gataaaagtc aagaattagt aatctattcg aactcagtct caaatggtcg
     1801 tggtgattgg ttaactgcta aagcaaaaga agctggtttt aatataaaaa tggttgatat
     1861 cgctggcgct caattagcag accgtgttat tgctgagaag aataatgcag ttgcagatat
     1921 ggtatttgga attggtgctg ttgactcaaa taaaattaga gatcaaaaat tactagtaca
     1981 gtacaagcct aaatggttag ataaaattga tcaatcttta tcagataaag ataattatta
     2041 taacccagtt attgttcaac ccttggtatt gattggagct cctgatgcaa aagaaatgcc
     2101 aaaagactgg acagagttag ctagtaaata taagggacaa tattccattt atggcttaac
     2161 tggtgggact ggacgcgcca tttacgcaag tattctagtt cgttatcttg atgaaaaagg
     2221 tgatttagga gtatcggaaa agggctggga agttgcaaaa gaatactttg caaatgccta
     2281 tacactacaa aaaggtgaaa gcacagttgt taaagtatta gacaaagaaa acgctatcca
     2341 atacggtatg atctgggggt ccggggctct agttggtcaa aaagaacaaa atgttgaatt
     2401 caaagttatg actcctgaga ttggtgtacc atttgtaact gaacaaacta tggttttaag
     2461 cactagtaaa aaacaagcgt tagctaaaga atttattgat tggtttggtc aatcagaaat
     2521 tcaagtagaa tatagtaaaa aatttggatc tattcctgca aataaagatg ccctaaaaga
     2581 gttacctgaa gatacgaaaa aatttgttga tcaagtgaaa ccacaaaata ttgactggga
     2641 agctgttgga aagcatttgg atgaatgggt agaaaaagct gaattagaat acgtacaata
     2701 agaaataaga gttatttttt atgtccaagt ctctctatta tttctaaatt taaggaagaa
     2761 ataaagagag gcttttggtt tagtaataga gggagttagt gagatgatta aatttgataa
     2821 tattcaaatt aaatatggtg attttgttgc aattgataat ctgaatttag atatacatga
     2881 aggggaattc tttacatttc ttgggccttc aggatgtggt aaatcaacta ctttgagagc
     2941 attggtaggt tttttagatc catcatcagg aagtattgaa gttaatggaa cagatgtcac
     3001 tcatttggaa cctgaaaagc gtggaattgg tattgtattt caatcttatg cgctatttcc
     3061 aactatgact gtttttgata atattgcatt tggtttaaaa gttaagaagg tagctccaga
     3121 tgttattaaa gctaaagtat cagcagtggc agcaaaaatt aagatctctg atcaacagtt
     3181 acagcgtaat gtatcagaat tatctggggg tcaacaacaa agggtagcat tggctcgtgc
     3241 tctggttctt gaacctaaaa ttctttgtct agatgaacca ttgtcaaacc ttgacgcaaa
     3301 attacgtgta gatttgagaa aagagttgaa aagacttcaa aaagagttag gtattactac
     3361 tttatatgtt actcatgatc aagaggaagc cttgacttta tctgatagaa ttgcagtctt
     3421 taacaatgga tacatcgaac aggtcggtac accagtagag atttatcata attctcaaac
     3481 tgaatttgta tgtgatttta ttggagatat taatattttg accgatgaaa cagtccacga
     3541 agtattattg aaaaatacaa gcgttttctt agaggataaa aaaggataca ttcgattaga
     3601 gaaagttcga ttcaatcgtg aaactgaaca agattttatt ctaaaaggga caattattga
     3661 tgttgagttt tctggagtta caattcacta tacaataaaa gtttctgaaa gtcagattct
     3721 taatgtaaca agtattgata gtcaggctgc tattagatct gtcggagaaa gtgtggaatt
     3781 atttatcaca ccatcagacg ttctgcaatt ttaaggaggt gcagtatgcg tcataaatta
     3841 aatttaaaag attggcttat tcgtttaggg ttaatctggt tcttagtaac atttattatt
     3901 tatccaaact ttgatctagt agtgaatgta tttgtaaaag gaggagaatt ttcccttgat
     3961 gctgtacatc gtgttctaaa atctcagagg gcacttcaga gtattatgaa cagttttaag
     4021 ttagcatttt cactcattat tacagttaat gtcgtaggta ttctttgtgt tctatttaca
     4081 gagtactttg atattaaagg tgctaaaatt ttaaaattag gttatatgac ctctttaatt
     4141 tatggaggag tggttttagc gactggatat aaatttgtct atggtcctta tggattgatt
     4201 acaaaatttt tacaaaatgt tatcccttct ttagactcta actggtttat tgggtatggt
     4261 gcagtcttat tcattatgac attttcagga actgctaatc atacattgtt tttaacaaat
     4321 acaattcgaa gcgttgacta tcacactatt gaggctgctc gaaatatggg agcaaaacca
     4381 tttactgttt tccgaaaagt agtgttacca accttaattc caactctatt tgcacttact
     4441 attatggttt ttcttagtgg tttatctgca gtagcagcac ccatgattgt tggtggtaaa
     4501 gaatttcaaa ctataaatcc aatgattatt acatttgcag ggatggggaa ttctcgtgat
     4561 ttagctgcct tacttgcaat tattttaggt attgcaacta caattttgct tactatcatg
     4621 aataagatag aaaaaggtgg aaattatatt tctatctcta agactaaagc gcctcttaaa
     4681 aaacaaaaaa ttgcgtctaa gccttggaat atcattgctc acattgtagc atatggattg
     4741 ttcacagttt tcatgcttcc actaattttt atagtattat actcatttac agatccagtt
     4801 gcaattcaaa caggtaactt aacattatca aactttactt tagaaaatta tcgcttattc
     4861 tttagtaata gtgcggcatt ctctccattc ttggtcagct ttatttattc tattattgct
     4921 gcgacaacag caacaattct cgcagttgta tttgctcgtg ttgtcagaaa acataaatct
     4981 cgttttgatt tcttatttga atatggtgct ctacttcctt ggttactacc aagtacgctt
     5041 ttagcagtaa gtttattatt tacttttaat cagccacaat ttcttgtctt gaatcagatt
     5101 ttggtaggta gtttggtaat tctacttatt gcatatatag ttgtaaaaat cccattttct
     5161 tatagaatgg tacgtgctat tttatttagt gttgatgatg agatggaaga tgcagcaaga
     5221 agtatgggtg cttcaccttt ttatactatg atgaaggtta tcattccatt tattttaccg
     5281 gttgttctct ctgttattgc tttaaacttt aactctttat taactgactt cgacttatct
     5341 gtattccttt accatcccct agctcaacca ttaggtatta cgattcgatc tgcaggtgat
     5401 gaaacagcaa catctaatgc acaagctctg gtatttgttt atacaattgt tctgatgatt
     5461 atttctggaa cggtattata cttcacacaa agaccggggc gtaaagtaag gaaataatca
     5521 tgacagccac tagtcttggg ttatcaaata ttgaaatagt tgtcaggatt gttttatcag
     5581 tagtcattgg tagtataatt ggtttagaga gagggagcaa atcccagcct gcaggcatcc
     5641 gaacttatag tattgtttgt ctagctgcat gtttgattat gatgacgaat gaatacgtat
     5701 cttataaatt tgggacagga gatcctacac gattaggagc tcaagttata tcaggtgtgg
     5761 gttttctagg cgctggaacg attcttatta cagataaaaa gaaaattaca ggtctgacaa
     5821 ctgcagcagg catttgggct tcggcaggaa ttggattagc tattggagta ggtttttatg
     5881 agggagctct tttagtagcc atttctgttt ggggtgtgat atccatgttc caaccactaa
     5941 aaaaatatct gcaaaatcgt tctaaaatga ttgaattgta tatagtggtt aaatctacag
     6001 aagcctataa tcgtgtattg gtttattgtg cagaaaatgg tatcagaatg accgattcaa
     6061 gaacagcgtt tggagatgtt aattctgaca gaattgaata ctttgatgtt ccggacaaaa
     6121 aaatagcgtc atttattact ctggaattat caggtagatt tgaacatctt cgactgatgg
     6181 aagaaattgc taatattgta ggtgttattt atgtcgaaga aattagctga aatttagtaa
     6241 acaaagggac agatgttttt gtccttttgt tttttagatg ttttaatcat ctaatttttg
     6301 agtagatttt tgctcagagg tttttatgaa agattataaa atcaatttt
//
LOCUS       DAVMEI010000099         6281 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVMEI010000099 DAVMEI010000000
VERSION     DAVMEI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.099"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            123..1502
                     /gene="glmU"
                     /locus_tag="VYQ24_001487"
     CDS             123..1502
                     /gene="glmU"
                     /locus_tag="VYQ24_001487"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1968613.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAEQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            1512..2057
                     /locus_tag="VYQ24_001488"
     CDS             1512..2057
                     /locus_tag="VYQ24_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1968614.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            2069..2383
                     /locus_tag="VYQ24_001489"
     CDS             2069..2383
                     /locus_tag="VYQ24_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1968615.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            2400..3092
                     /locus_tag="VYQ24_001490"
     CDS             2400..3092
                     /locus_tag="VYQ24_001490"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1968616.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            3169..3660
                     /gene="rocS"
                     /locus_tag="VYQ24_001491"
     CDS             3169..3660
                     /gene="rocS"
                     /locus_tag="VYQ24_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1968617.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            3758..4348
                     /locus_tag="VYQ24_001492"
     CDS             3758..4348
                     /locus_tag="VYQ24_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1968618.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            4357..6120
                     /locus_tag="VYQ24_001493"
     CDS             4357..6120
                     /locus_tag="VYQ24_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1968619.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         1943 a         1063 c         1373 g         1902 t
ORIGIN      
        1 aatcaaaaaa aatcaaaaaa atgggttaag gaagagactt tagagcattt tttcattcaa
       61 gagtgcggaa tgatttgaaa tatggtataa taaaagggaa tttctacaga aaagagaaga
      121 ttatgtcaaa ttttgccatt attttagcag cgggtaaagg gactcgcatg aaatctgatt
      181 tgccaaaagt tttgcacaag gttgcgggta tttctatgtt ggaacatgtt ttccgtagtg
      241 tgggagctat ccaacctgaa aagacagtaa cagttgtagg acacaaggca gaattggttg
      301 aggaggtctt ggctgaacag acagaatttg tgactcaatc tgaacagttg ggaactggtc
      361 atgcagttat gatgacagag cctatcttag aaggtttgtc aggacacacc ttggtcattg
      421 caggagatac tcctttaatc actggtgaaa gcttgaaaaa cttgattgat ttccatatca
      481 atcataaaaa tgtggccact atcttgactg ctgaaacgga taatcctttt ggctatggac
      541 gaattgttcg taatgacaat gctgaggttc ttcgtattgt tgagcagaag gatgctacag
      601 attttgaaaa gcaaatcaag gaaatcaaca ctggaacata cgtctttgac aacgagcgtt
      661 tgtttgaggc tttgaaaaat atcaatacca ataacgctca aggcgaatac tatattacag
      721 acgtcattgg tattttccgt gaaactggtg aaaaagttgg cgcttatact ttgaaagatt
      781 ttgatgaaag tcttggggta aatgaccgtg tggcgcttgc gacagctgag tcagttatgc
      841 gtcgtcgcat caatcataaa cacatggtca acggtgttag ctttgtcaat ccagaagcaa
      901 cttatatcga tattgatgtt gagattgctc cggaagttca aatcgaagcc aatgttatct
      961 tgaaagggca aacgaaaatt ggtgctgaga ctgttttgac aaacggtact tatgtagtgg
     1021 acagcactat cggagcagga gcggtcatta ccaattctat gattgaggaa agtagtgttg
     1081 cagacggtgt gacagtcggt ccttatgctc acattcgtcc aaattcaagt ctgggtgccc
     1141 aagttcatat tggtaacttt gttgaggtga aaggatcttc aatcggtgag aataccaagg
     1201 ctggtcattt gacttatatc ggaaactgtg aagtgggaag caacgttaat ttcggtgctg
     1261 gaactattac agtcaactat gacggcaaaa acaaatacaa gacagtcatt ggagacaatg
     1321 tctttgttgg ttcaaattca accattattg caccagtaga acttggtgac aattccctcg
     1381 ttggtgctgg ttcaactatt actaaagacg tgccagcaga tgctattgct attggtcgcg
     1441 gtcgtcagat caataaagac gaatatgcaa cacgtcttcc tcatcatcct aagaaccagt
     1501 aggagcctat catggagttt gaagaaaaaa cgcttagccg aaaagaaatc tatcaaggac
     1561 caatatttaa actggtccaa gatcaggttg aattaccaga aggcaaggga actgcccaac
     1621 gggatttgat tttccacaat ggggctgtct gtgttttagc agtaacggat gaacaaaaac
     1681 ttatcttggt caagcagtac cgcaaagcta tcgaggcagt ctcttacgaa attccagccg
     1741 gaaaattgga ggtaggagaa aacacagccc ctgtcgcagc tgcccttcgt gaattagagg
     1801 aagaaacagc ctatacaggg aaattagaac tcttgtacga tttttattca gctattggct
     1861 tttgtaatga gaagttaaaa ctatatttag caagcgattt gacaaaagtg gaaaatccgc
     1921 gtccgcagga tgaggatgaa accttggaag tccttgaagt gagcttagaa gaagcgaaag
     1981 aattaatcca atcaggtcat atctgtgatg ccaagacaat tatggctgtt cagtattggg
     2041 agttgcagaa aaaatagagg aggtcagtat gggtaaatct ttattaacgg atgaaatgat
     2101 tgaaagagct aatagaggcg aaaaaatttc aggtcctcct ttgctagatg ataatgagga
     2161 gactaagatt ttaccaacct cttcttcccg ttttggttat gccaatccta aggatcatgg
     2221 ttttagccag gaaaccttga aaattcaggt cgaaccatct attcataaaa gccgtcgcat
     2281 tgaaaatacc aagagaaatg tcttcaattc taagttgaat aaaatcttat tcgcggtcat
     2341 ctttctcttg attttgcttg ttttagcaat gaaacttttg taatagaaaa ggaattgaaa
     2401 tgaaaatagg aattattgct gctatgccag aagaactggc ttatctggtc cagcatttag
     2461 ataataccca ggagcaagtt gttttgggga atacctatca tacaggaacc attgcttctc
     2521 atgaagtcgt tcttgtagaa agtggaattg gtaaggtcat gtctgctatg agtgtggcga
     2581 ttttggctga tcatttccag gtggatgccc ttattaatac gggttcagct ggggcagtag
     2641 cagaaggtat cgctgttggg gatgtcgtga ttgctgacaa attagcctat catgacgtgg
     2701 atgtcacagc ttttggctat gcttatggac aaatggcgca acaaccgctt tatttcgaat
     2761 cagacaaaac ctttgttgct caaatccaag agagtttatc tcaattggac caaaactggc
     2821 atcttggttt gattgctaca ggagatagtt ttgttgcagg aaatgacaag atagaagcga
     2881 ttaagtccca tttcccagaa gttttagccg tggagatgga gggggcagct attgctcaag
     2941 cagcgcatgc cctcaatctc ccagtcttag tcatcagagc tatgagtgac aatgccaacc
     3001 atgaagcaaa catctttttt gatgagttta ttatcgaagc tggacgtcgc tctgcccaag
     3061 tcttgttgac ctttttgaag gctttagatt aagcggaaat ttgacagttt ttctagctta
     3121 tgataagatt taagtaaaga aaagctagaa aacgtttcag aggatattat gagtattgaa
     3181 atgaccgtca gtgagattgc agaggtctta ggattatctc gccaagcaat caataaccgt
     3241 gtcaaagaat taccagaaga agacacagat aaaaatgaca aaggggtaac agttgttacc
     3301 agaagtggct tgattaagct agaagaaatc tataaaaaaa cgatttttga agatgagcct
     3361 gtcagtgaag atgtcaaaca acgtgaactg atggagattc tagtggatga gaagaatgca
     3421 gaaattcttc gtctttatga acaattaaaa gccaaggatc gtcagttatc agaaaaagac
     3481 gagcagatgc gtatcaaaga ccgtcagatt gctgaaaaag acaaacaatt agatcagcaa
     3541 caacaattga cccttcaagc catgaaggat caagaaaatc ttaagctaga gctggaccaa
     3601 gcaaaagaag aagtccaatc cactaagaaa ggcttttttg ctcgtttatt tggaggataa
     3661 tcaaggagct gtttaggtta aatgctaacc tgatggcttc ttttgtttgt gaatagtata
     3721 gttgattaga acagagacag tagaggagag gtacagaatg gaacgattag aagattacat
     3781 tgcttttgac ttagaattta atcagcacga aggacaaaca cacttgattc aagtatcggc
     3841 agtgcgtttt agagatggtc gggaaatcga tgcctatgat tcctatgttc ataccagcgt
     3901 ccctctaaaa agctttatca atggtttgac cgggattaca gctgagacct taaaagatgc
     3961 tcctccagtt gagaaagtct tagaagaatt ccaagagttt gtgggttctt tgctgatgat
     4021 aggctacaat gctgctaaaa gtgatttacc tattttagca gagcatggtt tagactatag
     4081 ccaacagtac aaggtggatt tgtatgatga agcttttgaa cgtcggagtt cagatttaca
     4141 tggtattgcc aatctcaaat tgcaaactgt tgcctcattt cttggatttc aaggtcagtc
     4201 tcataatagt ttagaggatg ctcggatgac ggcgcgtgtt tacgaatcct tcttagagtc
     4261 ggatcaagct agggaattgt tagagacaga aagtagctta tcaaacaatc cttttggtgg
     4321 cttggacttg tctcaattat ttgactagga gtgcctatga aacctgaaaa acctaaaaaa
     4381 ttgggtctta aacagattta cctcaatctt gataaaatcc tctttctatt tttcttgatt
     4441 tttatgatag tggagtattt gtgggtacca ttcaattcat ggttggctag cttcttatta
     4501 gatcagacag gttatctttt tatttcctat aacaatgtct tagccatcct tactcattct
     4561 ccattgatta gtctagcttt tttagccttg attgtaatta accttctggt tgcttatttt
     4621 caaataggac tcctctttat tggtgctcgt catctcctct ataatgaaaa gcgaaggcta
     4681 attgagtata tccgcaaggt tttccatgaa agttttggat ttatgaaaaa gctaacttgc
     4741 gctaaagcct tgtttatctt tttctatata gctatgcttt ttccttttat acggaaaatg
     4801 ctgaagattt attatcttaa taaaatcgtc attccagagt ttatccaagc ttatttagaa
     4861 gataaatatt ggatgtggtg gctgagcatc ctacttctat ctcttatctt tctctatgtc
     4921 tctgtcagat tgatgtttgc ccttccgaag attgtatatg atcagctgac tgttcgagaa
     4981 gcagtgatgt ttagcttgga gaaaaccaag aagagagtta ctttttatgc ttggaattta
     5041 ttttacatct tgctcaaagc acatttatta ttttatcttc ctttgattcc tttattattg
     5101 gctcaaactt tggtagatga tatcactcag agagagtctc tgattcttgg tattttgaat
     5161 tttgtcgtga ttaaaaatct ctattatatg gctctgactt attttttggt taaatttgtt
     5221 tcatttttga caggaaagga gctggatatg cttcctagga gagaaaaaga tcacatcgtg
     5281 cgatggggtg tcatgacttg tgccagtctt ttctttgcct tagaaggtta tatttatctg
     5341 gaggctccca tggttcatct acctcaactt atttctcacc gaggggtttc caatgcaaat
     5401 gggattcaaa atacagtaga gtccttggaa actacagcac aactcaaacc agacttggtg
     5461 gagacggacg tgcaggaaac aaaagatggg cagtttgtca tgatgcatga tgctaacttg
     5521 aaaaatctag caggtatcaa taaaagtcct caagacttaa acttggagga gcttaaaggg
     5581 attgatattt ttgaaaatgg ctaccagact aaaatttcaa gctttgaaga ttatctcagt
     5641 cgagccaacg aacttggtca aaaattacta attgaaatta aaaccagtaa aaaagatagt
     5701 ccagacatga tgaaccgctt tttagcccgt tatgctgcaa agctcaagat ttatggacat
     5761 caaatccagt ctttagacta ccatgttgtc gaaaaagtaa gacagtatga tgccgaactg
     5821 ccagtttatt tcatcatgcc ctacaattct gtctttccta aaacaagagc gacaggatat
     5881 actatggagt actcaacctt ggatgaatat tttgtaagca aactatggac aacggatcag
     5941 aaactttatg tctggactgt caatgattca gaagctatca gcaaatctct tcacttagga
     6001 gtagatggag tgattacaga tgatttggaa aaagttcagc gggaaataga agtagcccaa
     6061 gaagatccag aatatacgga tttgctcttg aaaaaagcat tggaattctt tgaattttag
     6121 taataaaaaa gaggaacggt tgttcctccc atgataggta agtattcttg tgaatgcttg
     6181 cttttttgtg gttgcaatcc gtttacgaga attgtttgta ttgttcttgt ttcaatttac
     6241 tttatagtgc tttgaagttg gaatgctaca ctataacttc t
//
LOCUS       DAVMEI010000100         6208 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVMEI010000100 DAVMEI010000000
VERSION     DAVMEI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.100"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(38..532)
                     /locus_tag="VYQ24_001494"
     CDS             complement(38..532)
                     /locus_tag="VYQ24_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968620.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVDLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(529..993)
                     /locus_tag="VYQ24_001495"
     CDS             complement(529..993)
                     /locus_tag="VYQ24_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968621.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKVIRLESPDELLSYFGMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(1003..2337)
                     /locus_tag="VYQ24_001496"
     CDS             complement(1003..2337)
                     /locus_tag="VYQ24_001496"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1968622.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(2348..2965)
                     /locus_tag="VYQ24_001497"
     CDS             complement(2348..2965)
                     /locus_tag="VYQ24_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1968623.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKLVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(3013..6111)
                     /locus_tag="VYQ24_001498"
     CDS             complement(3013..6111)
                     /locus_tag="VYQ24_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1968624.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREKDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
BASE COUNT         1740 a         1437 c         1190 g         1841 t
ORIGIN      
        1 aagtcaaaaa aaagcaatct atgatacttt tcctaactta ttttttcatt tcccagacca
       61 tatcatacca tgtttcccct gcaaaggttg actgggactg gccttcgttg acaaatccat
      121 gtttttcata ataggcgatg agatagtcat gacaggttag attaatgcca tctctctcat
      181 cttcaagagc aacttctttc aaggctgtca gcaatttctg acccagtccg agtccctgtg
      241 cctccttggc aatagacaga caggtcacag agatataacc accaggctca tgactatagt
      301 cttttatttc ttctgtaaaa gactggtctt gcagatggcg gtgcaggtca actggtcctt
      361 cgatataacc catgattctt ccttcttttt cagcaaccag aaaagaggtc tgaatttctc
      421 tcaaatgtgc ttcaaagaca gaaggaggaa tggcttcttc gaccgaaaaa ttttcaaatt
      481 caagttcaac aatccgatcc aaatcttcta atcttgcttg tctgattttc attgttcctc
      541 cagataaaag ggattaaacc aaatcatact atagccctgg ctagttgcat agagcaaagt
      601 ttcttcttca tcaacaaaac cgttcattcc aaaataggaa agcagctcat caggactctc
      661 caaacgaatc actttgtaat ccagctcaac tgccacctct ttcaaggctg caagaagaag
      721 tgttcccagg ccctgtctct gatggtcaga ctcgatgact aaagaatgta cttttagaca
      781 ttgcggattg tctgactggg gacttgataa aatatagcct aaaagttgat tttcatccct
      841 agctagaaga aaggtatccg cacacttacg gatactttct tctaaaatat gggaaagttg
      901 ctgcttttca gctggaaaag acgaggtctg aagtgcccct atctcaggca aatcagactt
      961 gcttgcctga ataatcttaa ttggaatttc catgggaaca tcctattgaa cattgcttgt
     1021 caagttagac aagagacgct caaatgagta ttcataggtt tggatgtctc ctgctcccat
     1081 aaagacgtaa acagcattgt catggtctag gagcggagaa acattttcaa cagtaatcac
     1141 ttggtgtttt ttgttgattt tgttggctag gtcttctacc ttaacgtcac catgatctac
     1201 ttcacgagcc gagccataaa tttgcgctag ataaacagca tctgcttggt ttaaagcatg
     1261 ggcaaagtcg tccaacaagg caatggttct tgtaaaggta tgcggttgaa agactgctac
     1321 aatttccttg cttgggtatt tctgacgagc cgcatccaag gtcgcaataa tttctgttgg
     1381 atgatgggca aagtcatcaa taatcactgt atcattgaca attttctcag tgaaacgacg
     1441 tttaacaccg gcaaatgttt tcaagtgctc acgcaccaag ttcaaatcaa atcctgctgt
     1501 gtaaagaaga ccaataacgg ctgtcgcatt catgatattg tgacgaccaa aggttggaat
     1561 gtggaattgc cccaagtttt gtccacggaa atgaacggtg aaggttgaac cagttgttga
     1621 acgaagaaga tcactagcta caaagtcatt gccttcagct tcaaaaccat aataataaat
     1681 tggtgcatca gacgtaatct tacgcaattc agcatcttca ccatagacaa aaagaccctt
     1741 ggtaatttgt ttggcatagt cgttaaaggc attgaaaaca tcctcgagac ttgtgaaata
     1801 atctggatgg tcaaagtcaa tgttggtgat aatagagtat tctgggtggt aaggcatgaa
     1861 gtgacgctca tattcgtcag attcaaagac aaaatatttg gcattagccg aaccacgacc
     1921 tgtcccatct ccaatcaaga agctggtatc tgtaatgtga gacaagacat gagacaacat
     1981 acctgtcgtt gaagtttttc catgtgctcc tgctactccc atgctaacaa agtcacgcat
     2041 aaagctacct agaaactcat ggtaacgttt gtagctgata ccattttggt ccgcataggc
     2101 aatttcgacg ttgttatctg gacgaaaggc atttccagcg ataatttcca tatcaccgtc
     2161 tagattcttt tcatcaaaag gaagaatggt aattcctgcc tgctcaagac cgcgttgggt
     2221 aaagtagtac ttttcaacat ctgatccctg aaccttgtgc cccatctggt gcaacatcaa
     2281 ggccaaggca ctcatccctg atcccttaat tccgataaaa tgatatgtct ttgacatgtt
     2341 ttctccccta ttctgtcatt ctggtcagat tcaactcttg ggcaacccga cgttcttgtt
     2401 ctgtttgttt acttttttta ttgtagattt ggctcttctt tagaaaatca taattgtttt
     2461 tctttggagc aggtgctgac acttcttcat tcttggtagg gatagaatga acttcttccg
     2521 ccaagatata atgagactgg gtcaattttt ggctatattt gacaaattca ccaggatttt
     2581 ccttttggaa aggagttgtc ggttgattgc cctgtctaac tagactgggc tgagaatgac
     2641 gtctcgcaag gctgaaatcc tgagttaggt agttagcaga gcgtttcttt ttcaagtccg
     2701 cacgcgcttc ttcacgcgcc acctccgcat agctctttcc ttctttttta acccctaaag
     2761 gagccttttt aggtttttcg acttgctttt caatcggttt tactagtttt tcttcagtaa
     2821 taggagccca ttctaaataa tttttatctc gatactcacc cttgatatta ctgatcagat
     2881 cagactcatc atagagattc atgactggca tttcagtcaa catgacctcg tcatctgaca
     2941 ccaatggaaa tcgttcttgt ttcattttct atttcctttc aacacttcat tatagcgtat
     3001 tgtcttgatt tttcaagtgc tggcttcaga aattcccaaa atttctctaa tttctgctag
     3061 ggtcagacta ccacgtgact ctgtaccatc caatacttgt gacaccagat gtttcttttg
     3121 ttcttggagt tcctgaattt tttcttcaat ggttcccttg gtcaccaagc gatagacctc
     3181 aaccgtttct tcctgaccca tccgatgggc acggccaatg gcttgcgctt ccaccgcagg
     3241 attccaccaa aggtcaacca agatcactgt atctgcacct gtcaggttca gaccgacccc
     3301 accagccttg agggaaatca gaaaggcatc tctttctcct tggttaaagg ccttggtcat
     3361 gtcttgtctt tccttggctg gggttgaacc cgtaatttta aaggatgtca ggcccaagtc
     3421 tggcagttct tgttcaattt tttccaacat tcccttgaac tgagagaaaa tcaagacacg
     3481 gtgtccgccg tctgccacct gtaccagtag gtctcggaga ctatctagtt tgccgctggc
     3541 tccctgataa tcttccataa acagggcagg agtgtcacat atttgacgca agcgcatcaa
     3601 accagataaa atttccacac gacttcgctg aaattcctgt tctgacactt gagccagatg
     3661 gtctcgcatc tgttgtaact gggcaaggta aatagccttt tgctggtctt ccagttcatt
     3721 tttataaacc acctcaatca agtctggcaa ttcagtcaga acttcttctt tcttgcgtcg
     3781 catcacgaaa ggcttgataa actgagccac tcgctctgct ggcaatttca taaattcttt
     3841 cttgcttggc aaaagtccgg gcatgacgat ttggaaaata gaccacaact cacccagatg
     3901 gttttcaatc ggagttcctg acaaggcaaa gaccgacggc accacaaatt gtctcaaggt
     3961 ctgggcaatc ttggtctggg catttttcat gacctgagcc tcatctaaga aaaggaagtc
     4021 aaaggccatc ccctgataaa actcactgtc ctgacggaag gtggcatagc tagtcacata
     4081 gatttgatgg ctctcggcaa gaatctcctc acgacttgct ttcaaaccat gaacaacagc
     4141 cacatccaac tgtggggcaa atttctgaaa ctcatctgcc cagttgtaaa tcaaacctga
     4201 cggagcgaga atcaaaaccc gactttcttt tgtcacttga ctagtcaaaa aagcaatggt
     4261 ctgaagggtt ttcccaagtc ccatatcatc agccaaaatc ccaccaaaac cataatgatg
     4321 gagcatctgc aaccagccaa ttcccttttc ctgataatct cgcaagtcag cctgaacctg
     4381 agttgcctgt cgaggaaagt cctctggatg tgtcaaatcc tgggccagat tctggaattc
     4441 ttgtgaaaaa gaaacacggt ctcgcccttc aaagagatga gctaaactgt aggccaagga
     4501 tttccgagcc tgcaaggtcc catcttttaa ttcaaattgc cccagttcct gtagattttg
     4561 ccgaattttc ttggtttctt catcgaaaaa gtaaacttga ttagacgaat caatataaaa
     4621 atcctgattg gcaaccaagg cctgcatggc ttggtcgatt tcctcctggg caatattttg
     4681 aaaatcaaac tggatttcca agagacctcc cttggaggca atctgcacct gaggactcgc
     4741 taggctataa agctcttcta gtttatctga taggtcaaca tgcccgagtt tttcaaagac
     4801 tggaatgata tcatgaaaaa aatgatagac agactccgct tttaaggcct gacgccaaga
     4861 ttgaaaatcg gcttcaaagc ccgcagccaa acagacttgg aaaattcttt cttctaagtc
     4921 cgcgtcactt gaaaatggta attcttctag ctcttgtcgg ctagatacct gtctatttcc
     4981 ataatcaaac tgaatttcta aacgaatccg attatctttt tccctgtcaa agtaaaaaga
     5041 gggcgcaaaa gttttgattt gtagacgttc tggagctgaa acggtgccca tctggataaa
     5101 aagagtcaga caggaggcca atttgtctcg atcactgcta tcaaattgca agtatttctt
     5161 tccttgttga cccacaggta acactttaat ttccttgaga agacgcatct gctggtctgt
     5221 taaaaaataa acctgacctt tatggaaaag tactgctccc tgataaaaga cattaaccct
     5281 aggactctca ctgatttcca tttcaaaata atccgagtat tctgttactg taaaggcaaa
     5341 tagattggca tcagcatgca tatcctgaaa aagcagggtt tggtagctat ccacttgatg
     5401 gtcaaattga aaatggggca aggccatcag taaattcaca ccctgctcaa aaaaggtcag
     5461 agggaaaaag aggtgccgac cttggttttg gaaaaagagg tctggaacca gcccttcctc
     5521 cgttagtccg tgcaagaaag tcaaaagttc ttggctggca tcatcaaagg cttcccaaga
     5581 aagagactcc tcataaatct tgccaatcat atacgacttt ctctgctcga caatccttaa
     5641 aaaaagtgga atatcccgaa tgacatagta tttttggcta ttgatttggc cgattctcag
     5701 agtccacaag atatgattgg ttcctgcttc cacctgaccc acagctgata actcataggc
     5761 gcattctgat tttggagata aaattcgatc caaaaacttg ccacccaagg tcaccttggt
     5821 ttcaacagcc tctttttctt catgaccttc ttccagactc cacaagattt cctgaccacg
     5881 ctcatcattt ttcagaaaat gctctagcgc tgccaaatgc acacagtagc ccctcttttg
     5941 aaaaaaatca caggcacaaa aaaccaaatc atcctctaaa ctatagcgca gttcttcttc
     6001 tgcaacgcga gcgtagagcc gattgttctt ttccttgatg atatcaacct taccagtttc
     6061 ataaagggca acaccttcga tacgaacttt ccccggaatc aatttagcca tattttcacc
     6121 tttaccttat ctttttatta taccatattt tctcctatga aaatagcctt ctaggaagac
     6181 ttttctccta gaaggctgga tttttaac
//
LOCUS       DAVMEI010000101         6063 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVMEI010000101 DAVMEI010000000
VERSION     DAVMEI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.101"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(266..1108)
                     /locus_tag="VYQ24_001499"
     CDS             complement(266..1108)
                     /locus_tag="VYQ24_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1968625.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(1184..1261)
                     /locus_tag="VYQ24_001500"
                     /pseudo
     CDS             complement(1184..1261)
                     /locus_tag="VYQ24_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(1364..2755)
                     /locus_tag="VYQ24_001501"
     CDS             complement(1364..2755)
                     /locus_tag="VYQ24_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1968626.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKMEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(2990..3367)
                     /locus_tag="VYQ24_001502"
                     /pseudo
     CDS             complement(2990..3367)
                     /locus_tag="VYQ24_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            3600..4577
                     /locus_tag="VYQ24_001503"
     CDS             3600..4577
                     /locus_tag="VYQ24_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1968627.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFRKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            4574..5656
                     /locus_tag="VYQ24_001504"
     CDS             4574..5656
                     /locus_tag="VYQ24_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1968628.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     SEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(5720..>6063)
                     /locus_tag="VYQ24_001505"
     CDS             complement(5720..>6063)
                     /locus_tag="VYQ24_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1968629.1"
                     /translation="CNTEWMNVFLEELSQAYPDDYLLFVMDNAIWHKSSTLKIPTNIG
                     FTFIPPYTPEMNPIEQVLKEIRKRGFKNKAFQTLEDVMNQLQDIIQGLEKEVIKPIVN
                     RRWTRMLFESR"
BASE COUNT         1802 a         1352 c         1037 g         1872 t
ORIGIN      
        1 tccttgataa attctatata gtataaaatt caaaaagaac cacctttcaa cacccaagcc
       61 ttgcgactag gggcgtcaga aacgcggttc caccctaatt tattacttca ttgtttttga
      121 aaatactaca gaaagcgcca gctcattatt ttgccctact tggctcccac tgtcccaagc
      181 tcgcttgaag aacgccataa gactttcttt cctgctgact attatatcaa aaattagaat
      241 aatgtacaat tctttttatg attttctaga aatccacatc atcaactcgt ggagcacccg
      301 attgaacagc aatcgttttt aaggtttctc tctcctcatg actcagttca attccaaagc
      361 aatcaagatt agcttgaata cgagaggttg tgacagattt tggaagtggt aaaaatcctt
      421 ctgccaagct ccaggccaag gctatctgag caacagactt tccgtgattt gctgctattt
      481 cttggacttg cttgctatca aacagttctc cttgtccaaa aggcccccaa gcttccaata
      541 aaattccctt ttcacgacag taagctacga cttcctcttg atacacacct ggcgccaagc
      601 gaacttgatt gaccgcagga acgatagttg cagtttcaag caaggcatcc aaatgatggg
      661 gaagaaaatt gctaacgccg atagcacgga ttttcccttc ttgatagagg tcttccatcg
      721 ctctccaaac ttccgcattg cgagttttcc atgcgtcatt ttctctgagc ggttttgggt
      781 tcggccaatg aatcaaatac aaatccaaat aatccaagcc cagtttttct atagattttt
      841 ccaaagcttg acgagtttgc tcataggttt gctgactatt ccaaagcttg gtagttacga
      901 acatttcttc acgtggaact ccgctatctt tgattgcttg accaacactt tcttcattct
      961 gataaatcgc cgccgtatca atatgacgat aaccagcctt caaggcttct aacactgcac
     1021 gataggcttc ttctccatcc ttagccttaa aagttccaaa tcccaatact ggaatttcta
     1081 ctccattatt taattgatag gtattcatat gcttctcctt ctattttata ctctcttcaa
     1141 accacatcaa cgtcgccttg ccgtaggtat tgttactgac ttcgtcagtt ctatccacaa
     1201 cctcaaaaca ctgttttgag caacctgcag ctagtttcct agtttgctct ttgattttca
     1261 ttgagtatta ttcatcaatt atagcataaa acatttttta agtagtacta tcttttgtaa
     1321 aaaataagtc tatcacatat ctgacagacc tattttttct tgcttattca tcacgactat
     1381 aatacagctc atgagctttt aggcgagtat tgagcatctc tggtatggtc acaaaggtat
     1441 aaccttgatt tttcaaatac tcaatgaccc ttggcaaggc attgactgtc ggactgtgaa
     1501 tatcatgcat caaaacgata gagccgttag ctacttgatg ctgaatttcc gtcaaaatag
     1561 ctgcttcatt tttactcttc cagtccagac tatccacatc ccacataata aagctcaaat
     1621 cgaggctatt gcgaatatca tctgtaatag ctccataagg cggacgcatg agtttggaac
     1681 tagaacccag cactttagtt agcacatcct cagtatcagt aatctgcttt ttagcttcat
     1741 caagagatag ttgcgagaga ataggatggc tccaactatg gtttccaaca acatgacctt
     1801 cagattttat cctcttcacc aagtcctcat tcccagaaac atttttccca agcacaaaga
     1861 aagtcgcttt aatatcatat ttagctaggg tctctaatac ctgcggggtc gttgctggat
     1921 ttggaccatc atcaaaggtt agagcgacaa ctttttgatg tttcttatca aagtaagatt
     1981 ggtacaaggc cgcatctttt tcgagtaagt acgaagattg gataacatca aagaaagcag
     2041 atactggcaa ggctatctct tctaaatttt caaccacagg acttggataa aggataatct
     2101 gactatcctt gtaatcaaaa ttccatgcag acaagtcttg gtcagagaag ttttttacaa
     2161 tctgctcact ctggtcttgc tccatttttt tatcctctat gaaggaggtc aactctttta
     2221 tcagctgttc cttagcctta ctagcatctg aaaacagttg gtcaagtgta aaaggttgcc
     2281 catcttctgt taagtgaacc tttcctagac tggtcttttc agtctcttca atcttaaaag
     2341 caactaaatc atagatttgt ttggtcacat tacgattaac gactcccttt aaacctgact
     2401 cttcttgctc tgtatagtag aaaacaagat tgtccttact ttctagcttg tccttaacat
     2461 cttgatttat cagctcccta acagaggaaa tcactttctc cccttggaga ggataatagg
     2521 caatcacttc ggcttgcccc tgacgaaaat gctccttctg atttccctca ctcaattgat
     2581 catctttctc ttttttgagc gattcaatct tttgctcaaa acttttttgc tggtagatct
     2641 taaaagcaat aaatccgcct aataggcaaa tagaaattgc taaaatacca atcaaagcca
     2701 ataatacatg tctcgttttc ccgtgtctgc cacgtcctag tctactttta ttcatattta
     2761 tatcataaca aatttgagca ataatttcaa tgatacaatt tggcaatcca caattcctct
     2821 agcaagccaa agcatcttac atcaactcca acatccgtat ctgtagagat tatgtagttt
     2881 tctatctatg gaatttctag tagattgaag ctagaatagt agacctatac ttctaaaaca
     2941 ttgttagaaa tcgatttgac tgtcctgatc gatttgtcct gttctcattt cattttacta
     3001 taaaactatg aaaagctagg tcatctgaga agtaattact atcgacaatt gcgatagcgt
     3061 atactagtgc gatttgtttg tagctctggt gggtatcacc ttcacgttgg cgaatttttt
     3121 ctaggttttg attaacctaa ctgcacagat aagcccatag gcgattgatg aaaaaattct
     3181 gatgatgaac ctgaatttcg atgataactt ggattccatt atctaactcc gccaagacgt
     3241 ctatactagt atagaaatcc tgcgctgagt agggcatgga aggcaagacg tgaatgttac
     3301 ttccctccaa aatcgtcaca ttttttgctg gcaagtccag catatcgcgg ataaattgac
     3361 aaatcattag ttgggctgat acccggatgt ctgagaatcc tccttttcct ccttctatta
     3421 tagcacattt ttaaatctag gtttgctaga ttctatgctt ctatctattt attcggaaaa
     3481 gaaagctttt cttcagaaga aagctaatag cttatttgaa attttctcct ctctgttgga
     3541 tagtcccgtc tatctatgtt ataatgaaag aaggatttaa aatcggaaaa ggagtattta
     3601 tgctaaaatt aggtgtcatc ggaactggcg ctatcagcca tcatttcata gaagcagccc
     3661 ataccagtgg agaataccaa ctggtcgcaa tctattctag aaaactagaa actgcagcaa
     3721 cctttgcttc tcgctatcag aatatccaac tattcgatca attagaagtc ttcttcaaat
     3781 cttcttttga tttagtctat atcgccagtc caaactcctt acattttgct caggcaaaag
     3841 ctgccttgtc tgctggtaaa catgttattc tcgaaaagcc agctgtcact caaccacaag
     3901 aatggtttga tttgattcaa acagctgaaa aaaataactg ttttatcttt gaagcagctc
     3961 gtaattacca cgagaaagcc tttactacta tcaaaaactt tttagcagat aagcaagtgt
     4021 taggagcaga tttcaactat gccaaatact cttccaagat gcctgacttg ttgactggac
     4081 aaacaccaaa tgtcttttca gaccgttttg ctggtggagc cctcatggat ttggggattt
     4141 atcccctcta tgctgctgtt cgtcttttta gaaaagccaa tgacgcgact taccatgctc
     4201 aacagcttga caatagcatt gacctaaatg gagatggtat cctcttctac ccagactatc
     4261 aagttcacat caaggctgga aaaaacatca cttccaatct tccttgcgag atttatacaa
     4321 cagatggaac cttgactctc aacacgattg agcatattcg ctcagctatt tttaccgacc
     4381 accaaggaaa tcaagtccag cttcctatcc aacaggctcc tcatacgatg actgaggaag
     4441 tcgctgcatt tgcacacatg atccagcaac cagatctgaa tctctaccaa acttggctgt
     4501 atgatgcagg atctgttcat gagctactat ataccatgcg ccagactgct ggcattagat
     4561 ttgaggcaga aaaatgaaaa ccaaactacc gactgaatgg caagaactga gtgaccaact
     4621 cggtttccaa gaattcaccc ccattcaaac tcaactattt gagccccttc ttgctggaga
     4681 aaacctccta ggagtgagcc aaaccggaac tggtaagacc ctagcgtatc tcctaccaag
     4741 tcttctcaga ctacaaaaga aaaaagccca acaactcttg attctagcac caaatacgga
     4801 acttgctgga cagatttttg atgtatgtaa aacgtgggca gaagctatcg gattgactgc
     4861 tcaactcttc ctttcaggtt caagtcagaa acgacaaatt gaacgcctta aaaaggggcc
     4921 agaaattctg attggaactc ctggccgtat ctttgaactc attaaactta aaaaaatcaa
     4981 gatgatgaat gtggaaacca tcatcctgga tgaatttgac caattactag atgattctca
     5041 gattcacttt gtagaaaaaa tcactcacta cgcacctcgt gaccaccaac tggtctacat
     5101 gagtgccacg accaagtttg accaagaaaa gattgtgccg aatacacgca ctattgatct
     5161 ctctgaccaa aaattggaca atatccagca tttctacatg caggtagacc aacgtcatcg
     5221 agtggatatg ctacgaaaac tggctcatgt agaggatttc cgtggtctag tcttcttcaa
     5281 tagcctgtca gaccttggga atgccgagga aaaactacag tatcgtgata tattgactgt
     5341 ttccctcgct agtgatgtta atgttaaatt tagaaaaatc atcttagaaa agtttaaaga
     5401 taaccaacta accctgcttc ttgcaactga ccttttggct cgtggaattg atatcgatag
     5461 cctagaatgt gtcgtaaact ttgatattcc tagagatagc gaaacctaca cacaccgtgc
     5521 tggccgtact ggtcgtatgg gcaaggaagg atatgttatc actctcgtca ctcattcaga
     5581 agaaatcaaa aaacttaaaa agtttgcaag tatccgagaa attgtcctaa aaaaccaaga
     5641 actctatatc aaataaagcc ccattgctct atacacatcc agtgtatgga gcttttttat
     5701 aagcaattca attttatact catctgcttt caaaaagcat tctagtccat ctccgattaa
     5761 cgatgggctt tatcacctcc ttctccagtc cttgtatgat atcttgaagt tgattcatga
     5821 catcttccaa agtttgaaag gctttattct taaatccacg tttacgaatc tctttcaaca
     5881 cttgttcaat ggggttcatc tctggtgtgt atggaggaat aaaggtaaaa ccaatattag
     5941 tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg aataaaagat
     6001 aatcatctgg ataagcttgt gaaagctctt ctaaaaagac gttcatccac tcagtattac
     6061 atc
//
LOCUS       DAVMEI010000102         6027 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVMEI010000102 DAVMEI010000000
VERSION     DAVMEI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.102"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            120..1889
                     /locus_tag="VYQ24_001506"
     CDS             120..1889
                     /locus_tag="VYQ24_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEV1968630.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHINVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATKAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
     gene            1913..3868
                     /locus_tag="VYQ24_001507"
     CDS             1913..3868
                     /locus_tag="VYQ24_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1968631.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQTYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKLEEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            3870..4307
                     /locus_tag="VYQ24_001508"
     CDS             3870..4307
                     /locus_tag="VYQ24_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1968632.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            4369..5505
                     /locus_tag="VYQ24_001509"
     CDS             4369..5505
                     /locus_tag="VYQ24_001509"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEV1968633.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
BASE COUNT         1852 a         1015 c         1232 g         1928 t
ORIGIN      
        1 aagccttttt gcccagcctc gtcaaaagta atgttttgac acaaaatctg tgacaaaact
       61 ttagttttaa aggtttttaa ctttgtatat actagtttta agaaaaggag gatgatctaa
      121 tggaagaaaa agtatcattg aaagtcaggg ttcaaaaact agggacatcg ctttcaaata
      181 tggttatgcc caatattgga gcatttattg cttggggagt attgactgcc ctctttatcg
      241 ctgatggcta tctgccaaat gaacagttag ctactgttgt tggtcctatg ttaacgtatt
      301 tattgccaat cctgattggt tacacaggtg gatatatgat ccatggccaa cgtggtgccg
      361 ttgtaggagc tattgctact gttggtgcaa ttacaggttc tagtgttcct atgtttatcg
      421 gagctatggt aatgggccca ctgggaggat ggactatcaa gaaatttgat gagaagttcc
      481 aagaaaaaat tcgtcccgga tttgaaatgt tagttaataa cttctcagct ggtctcgttg
      541 gttttgcatt attgcttttg gctttctacg caatcggtcc agtcgtatcg actcttactg
      601 gagctgttgg gaatggtgtt gaggctattg tcaatgctcg cctccttcct atggctaata
      661 ttatcatcga accggctaaa gtccttttcc tcaataatgc cctcaatcat ggcattttta
      721 ctcctctggg agtagaacag gtagctcaag ctggtaagtc aattctcttc ctattggaag
      781 ctaatcctgg accaggcctg ggaattctat tagcttatgc tgtattcggt aaaggttctg
      841 ctaaatcttc ttcttggggg gcaatggtta ttcatttctt cggagggatt catgaaattt
      901 actttcctta tgttatgatg aagcctactc tatttttagc tgctatggcg ggaggtatct
      961 ctggaacttt tacttttcaa ctcttagacg ctggtcttaa atctccagct tcaccaggtt
     1021 ctattattgc gattatggct acggcgccaa aaggtgtttg gccccatata aatgttcttc
     1081 taggtgtttt agtagcagca gttgtttctt tccttgtagc agcccttatt cttcatgcag
     1141 acaagtcaac tgaggattcg ctcgaagctg ctcaggcggc taccaaagca gctaaggctc
     1201 agtctaaagg tcagttagta tcaacttctg ttgatgcagt tgtttcgaca gactcagtgg
     1261 aaaaaatcat tttcgcctgc gatgctggta tgggaagctc tgctatggga gctagtattc
     1321 ttcgagataa ggttaaaaaa gcaggtctag agattccagt atctaatcag gcaatctcaa
     1381 atttgcttga tacaccaaaa acattaattg ttactcagga agaactgaca ccaagagcta
     1441 aagacaagag tccaagtgct attcatgttt ctgttgataa tttcttagcg tcccctcgtt
     1501 atgatgaaat tgtagcttca ttaacaggag cttctccaat agcagaaatt gaaggagata
     1561 taccaacttc agcaccagta gatagtcagg aaattgacct taaccatatt gatgctgtag
     1621 tagttgctta tggtaaagca cagggaactg caactatggg ctgtgaaacg attcgggcta
     1681 tctttagaaa caagaatatt cgtattccag tttctactgc caaaatttca gaattaggtg
     1741 aatttaattc taaaaacata atgattgtaa caactatttc tttacaggca gaagtgcagc
     1801 aagcagcacc gaattctcaa tttcttattg tggatagttt agtaacaaca ccagaatatg
     1861 acaaaatggc tgctagaatg tacaaataga actagaggtt tctaaattac gaatgctatt
     1921 aaccaaacga gaagaacaat tattgaaggc tttcctacat gtagggaagc tttcaatgca
     1981 agatatgact gaaatcttac aggtttcatc tagaacaatt tatcgaactt tatcagattt
     2041 gacagatagc atggagcaat atggaatcga aataacgaag catgggaaat actatatttt
     2101 gactggagag ttggatgatt tgccgacaga acttgaagtg ttagttgagt atagtcccca
     2161 agaaagacaa gagttgatta cctatcgcct tctgactgag agtggttttg tcaccaatga
     2221 agcattgcaa gagtgcacga aagtcagtaa tgtaactatt attcaggata tttcagatat
     2281 tgataagcgt cttttagact ttgatctgaa aattgaacga caaaaaggtt atcggatttc
     2341 tggtgattca gttggtaaga gaagattttt ggctatttta ctgacaaact gtatctcagt
     2401 agcagatttt tcaaccggta attttgggag ctttgatatt ttagaagcag atagaactag
     2461 gctggccagt cagattgtta ataagcaact gtcaggtttt ccagatatgg atgctaggat
     2521 gaagatgttt tttgcgatct tgttatctct tataggtcag gagcaaaaca ttgaaaattc
     2581 acctaatact agtaagcagg ctttggaaat ttctcaaaaa atttttcaaa cttactctaa
     2641 gcagactgca caattttata gtattcagga aattatctat tttgcgagca tcttggatga
     2701 attaatcatt aaacgtcagg acaatccgct ctttacggag aaatttgatg gtgaattttt
     2761 ctacaatatt tcaaatctga ttgatacggt ttccatgtat accaagattg acttttttaa
     2821 ggacaaggtt ttattcaatt ttcttttcca tcatattcgg ctcagtttag gcgtccctat
     2881 cctttttcag ggtgaaaatt tgccagaatc tgtccagatt ttagttgaaa ggaataaatt
     2941 tctttataca gtcatcagtc ttttagtgaa tgatattttt ccgaaatatc ttcatacaga
     3001 gtatgagtat ggcatgattg ccctacattt tatctctagc ttaggccgta gtccagagat
     3061 ttatccagtc cgtgttttgc ttttaacgga tgaacgtcgg gtcactagag atttattagt
     3121 cagtaaaatt aagagtgttg ctccttttgt agagttgata gatattcagt ctctagtaga
     3181 ttaccacagt attgatctca gtcagtatga ttatatttta tctaccaagc cgctgactaa
     3241 tcaggaaatc gatgtaattt ctagttttcc aaccgtcaaa gaattgcttg aattacagga
     3301 acgacttcag tatgtacagg cacatcgtac aattgtcgcg cgtgatgcta tcgctccaga
     3361 gaaaagttat gacttgcaag attatttaat atctagtagt cagcttttga gtcaattcga
     3421 gttggttcaa ttggagaata atcaatcatt tgagcacacg gtagaacaaa tcatccaata
     3481 tcagaagaat gtgagtgaca gagattacct aacaagaaaa ttgttatctc acttccagaa
     3541 tagtcctatg gctattccta atactggtct ggtgctttta catagtcagt ctagcaaagt
     3601 aacaacaaat agttttacta tgtttgaact caaactacct atctccgcat tgtcaatgaa
     3661 actagaggaa gaagaggtca aaaggtgtct gctaatgcta atgtctaaag aagctagcga
     3721 ggaagcgaga gatttaatga cagctattag tcagtcgatt attgaaaatc atctttatac
     3781 agagatttac aagacgggaa atcaatccat tatttatcag atgctaaata ctatttttaa
     3841 cgaaaaaatt aagaaattgg agaactaata tgaaacttga aaaacatttg attaagctta
     3901 ataaacaatt ttctaacaag gaggaagcta tttgttattg tgggcaagtt ctttatgagg
     3961 gtggatatgt taatgaagac tatattgaag ccatgattga gcgagataaa gagctatctg
     4021 tttacatggg taactttatc gccataccgc atgggacaga tgcagcaaaa aatgatgtcc
     4081 tcaagtctgg tattacagtc gttcaagtcc ctagaggggt tgattttggg aatgtatcta
     4141 accctcaagt ggcaacggtt ctttttggta ttgctggtat tggtaatgaa cacttagaaa
     4201 ttattcagaa aatttctatc ttctgtgcag atgtagataa tgttcttaaa ctagcagatg
     4261 ctcagtcaaa agaggaagta ttgcgcttat ttgatgctgt tgaataattg aatttcgtca
     4321 tttgtcatct agtatatatg tccctcaaat aggaaaagga gaaattgaat gaaacattct
     4381 gttcattttg gtgccggtaa tatcggtcgt ggttttatag gtgaaattct atttaaaaat
     4441 ggtttccata ttgattttgt ggatgtcaat aatcagataa ttcatgctct aaatgaaaag
     4501 ggcaagtatg aaattgaaat tgcacagaaa ggacagtctc gtatagaagt aactaatgtg
     4561 gctggcatta atagcaaaga acatcctgag caagtcattg aagcgattca aaagacggat
     4621 attattacta ctgcaatcgg acctaatata ctccctttta tcgccgaact tctagccaaa
     4681 ggaatcgaag ctcgccgagt tgcaggaaat acacaggcat tggatgttat ggcctgtgaa
     4741 aatatgattg gcgggtctca atttctttat caagaagtca agaaatattt aagtccggaa
     4801 ggtttgacat ttgctgataa ctacataggt tttccaaatg ctgcagtaga caggattgtt
     4861 ccagcacaaa gtcacgaaga ttcccttttt gttgtggtcg agccctttaa tgaatgggtc
     4921 gtggaaacca agcgtcttaa aaatccagat ttacgtctaa aagatgtgca ttatgaagaa
     4981 gatttagaac cctttattga gcgaaaactt ttttcagtca attctggaca tgcaacttca
     5041 gcttacattg gtgcgcatta tggtgccaag acaattttgg aagctcttca aaatcctaat
     5101 atcaaatctc ggattgaatc tgtattagct gaaattcgga gtctcttgat tgccaaatgg
     5161 aactttgata aaaaagaatt ggagaattat cacaaagtca ttatagaacg gtttgaaaat
     5221 cctttcatag tggacgaggt tagtcgcgta gctcgtactc caatccgaaa attaggctat
     5281 aatgaacgat tcatccggcc gatacgtgaa ttgaaagaac tcagtttgtc atataaaaac
     5341 ctacttaaaa cagttggcta tgcctttgac tatcgcgatg taaatgatga agaaagtatt
     5401 cgattaggtg aattgttggc taaacaatca gtcaaagatg ttgttataca agttacaggt
     5461 ttagacgacc aagaattgat tgagcaaatt gtagagtata tttaatcttt ttcgaaaatc
     5521 tcttcaaatc aggttagcat ccatctgcaa cctcaaaaca gtattttgag ctgacttcgt
     5581 cagttctatc tacaacctca aagcagtgct ttgagctgac ttcgtcagtc ttatctgcaa
     5641 cctcaaaaca ctgtttgagt tatctgcggt aatctttcta gcctgtcttt gatttttgtt
     5701 gttatttata aggtaaaaga agctggacaa aaagtcttca aaatcggaaa aaggcagcct
     5761 atcgggtgtt caaaaatctt gataggatgt cctttattat ggaaagcctt attggatttt
     5821 ctcctcagat tgagtttttg atcagcttta tgagataggt tttgctagag atgtagccca
     5881 tcatgttatt tttatggacg gtgggaaaat tgttgaaaaa aataatgccc atcaattctt
     5941 tagtcgtcca agagaagaac gaaccaagca attttggaac gaattctttc gaatgcgatc
     6001 tatatagtaa aatgaaacaa gaacagg
//
LOCUS       DAVMEI010000103         5930 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVMEI010000103 DAVMEI010000000
VERSION     DAVMEI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.103"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            57..824
                     /locus_tag="VYQ24_001510"
     CDS             57..824
                     /locus_tag="VYQ24_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1968634.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDSYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            909..1247
                     /locus_tag="VYQ24_001511"
     CDS             909..1247
                     /locus_tag="VYQ24_001511"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968635.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKS"
     gene            1275..1589
                     /locus_tag="VYQ24_001512"
     CDS             1275..1589
                     /locus_tag="VYQ24_001512"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1968636.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(1690..4242)
                     /locus_tag="VYQ24_001513"
     CDS             complement(1690..4242)
                     /locus_tag="VYQ24_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1968637.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4304..5926)
                     /locus_tag="VYQ24_001514"
     CDS             complement(4304..5926)
                     /locus_tag="VYQ24_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1968638.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         1988 a          980 c         1100 g         1862 t
ORIGIN      
        1 aaactaagtt taaagaatgg aaagcaattt aaaaaaaacc aacctttatt attgtcatga
       61 tcgggatttc tcttattcca gatctgtaca atatcatatt tttgtcatca atgtgggatt
      121 catatgggca attgtctgac ttacctgtgg cagttgtaaa taatgataaa gaggcttcct
      181 ataatggtaa tactatggca ataggaaaag acatggtgtc caatttaaaa gaaaataaaa
      241 ccttggattt tcattttgta gatgaagagg aaggaaagaa gggattggaa gatggcgatt
      301 actatatggt agtgacttta ccaagtgatt tatctgaaaa aacaactaca ttatccaata
      361 ttcaatcgac agcagcttat caatcattga caagtgagca acaaactgag ataagtgatt
      421 ctgtatctca aaattcaact gatagtattc aatcggctca gtcaattgta gctttagtac
      481 aagatttaca gggaagttta gaaaacttac aaaatcaatc ttctaatctt tcgactttaa
      541 aaaatcaatc taatcaagta tcacctatta cttctacttc tttgatagga ttgtcaagtg
      601 gattaacaga gatacaagga gatgttacta gcaaattagt tcctgccagt cagtcgattg
      661 catcaggtgt aaacgcatat actacaggtg ttgataaagt ttctcagggc gcaagtcaac
      721 taagtgaaaa aaatgccacc ttgacaggta gtttggataa actagtttca ggctcaaaca
      781 ccttgacaca aaaatcttct agattgacag caggagttgg ttaattacaa tcaggatctg
      841 ggcaattagc agacaaatcc agtcagttac tttcaggtgc ttctccatta gagaatagag
      901 ctaataaatt ggcagatgga tctgggaaac tagcagaagg tggaacaaag ttaacttctg
      961 gattggaaga tttacagaca ggacttgctt ctttaggaca aggactaggt aatgctagtg
     1021 atcaactcaa atcagtatca acagaatcta aaaatgcaga gattttgtca aatccactca
     1081 atctttcaaa aacagacaat gatcaagttc ctgtaaatgg aatcgcaata gctccttata
     1141 tgatatcagt tgctcttttt gttgcagcaa tatcaacaaa tatgatattt gcgaaattgc
     1201 cttcaggacg tcatccagag agccgttggg cttggttgaa atcttgagct gaaataaatg
     1261 gtattatagc tgttttggca ggaattttgg tatatggagg agttcagctt attggtttaa
     1321 ctgctaatca tgagatgaga atatttattc tcatcatcct aacaagttta gtattcatgt
     1381 ctatggtgac cactttagca acgtggaata gccgtatagg agcttttttc tcacttattt
     1441 tgcttttact acagttagca tcaagtgcag gtacttatcc acttgctttg acaaatgatt
     1501 tctttagatc tattaatccc tggttaccaa tgagctattc agtttcggga ttacgacaaa
     1561 caatctctat caacaagtca ttttcctagc tgtcatacta gttctattta ctagtttagg
     1621 tatgctagcc tatcaacata agaaaatgga agaagattaa aaaaatcgac cgattaactg
     1681 gtcgattttt tatgccttag atgactttcg tctgtgatta tagattccaa atagtaagag
     1741 agaagtaaag gaacagattg ctccagtaat aaaaccattg ggaatgaagg aaagtgtaat
     1801 agttcctttc cccttgggaa tgtcaacttt cataaatcca gtttgagctt gtttaatttc
     1861 tattttctta ccatcttggt aggcagacca acctttgtca taaggaatgg tgaagaaaat
     1921 agatgtatct tgttggacat catatgtagc aaaaaccttg tttttagaag ttgatactgt
     1981 gacaggttgt tctttaattt tttgaattgc ctcggtgaaa gttttggtat ctaaacgata
     2041 gaaggtagga gattcaaatg atacttgtga atttccaggg aaactaacat tgatattgaa
     2101 agtttttttc tctttagtat atcctagatt aaagaaggag aagacattat cagttgtaaa
     2161 agtctttttt tcaccattta caaggatgtc aaccttcttt tgtttatcgt tagaaaagtg
     2221 aaggtttatg aaagagagat aaacttggct gttttctgga acttcaattt gatactggat
     2281 tgctgcatct tcatttgaag aacttgtgac actaatcaaa tcattagtat tttctatttt
     2341 ttctgttttt tcataaggta ttggagaaaa ataatcaaaa ttgacgttag caagttgatt
     2401 taaaaatgag gcctgattat ccaaggtatg ttcattgaac ttgacatcat tgtaaacaga
     2461 ttgactagca actgcaatcg gaagagagta ttgattttca tatagggtaa gattatcttt
     2521 ttgatagata tctttaaagc catacttatc aataggactg tctgagatat tgtactggat
     2581 accaaataaa ctatcagcca aaatactatt atttgcatat cggagattga gattagtccc
     2641 agaggattta aaaccaagtt tatctaaagt agagcttgat gaacgatttc gaacagatga
     2701 aaattgagag attccattgt agttgaattt catactgtca tttcctgtct gagtttgtag
     2761 tttttcagta cgagtaaatt gatttccaat atatgttgag aaagattcca tagctgggat
     2821 atctcgacta taagcacttc gagaagcaaa tccccattcc ttagcaattc cgtccatttg
     2881 agatgaagca tttaaactca tttcaaccag tataaataaa gagattagaa tggcaaatag
     2941 attcacagat ataaactttt tgataactgc aaggagtaaa agagaataga caaccaaaaa
     3001 ttcaagagta agcagaatat tcaaatctgt taaaaaagaa taatgcgatt ttagatagat
     3061 ggtagctaaa aatcctgtta ctacaagaaa aagcgaaact aaaaaattcc agactttaag
     3121 ttctttcaga cgctttaaga cttctgctgc tgtgtaaatt aacaaggtag agaaaatcca
     3181 agcatagcga tgtaaaaaca tgtttggagt atgcatgcct tgccaaaata agtcaagagc
     3241 ttctatgtaa aagcttgcaa ttagaaatgc aaagaatatt acatatatga gtttcacgtg
     3301 aaacttaata gatttcagcg taaaaaataa aatggtcaaa ataaagggaa atagtccaac
     3361 aaaaatcatt gggatggccc catactttgt tgtgtcaaag gaaccaatga attgcttagc
     3421 aaagagatca agataccagc tactttcagt ttgaaacttt gtaacttcag tcaatttttc
     3481 cccatgtgtc tgtaaatcaa atagagtggg aagagtcata atcaaactag ccataccagc
     3541 taaaaaggag ataactatga aatcaagaac agatgatttt cgagtcttaa agtcccacga
     3601 aatttgacag agataccaga aaataagaaa caatactgtc atatatccaa aataataatt
     3661 ttgaataaat aagattgaca gacttgtaaa gtacaatagg agtttctttt cagttatcag
     3721 tagatgtaaa ccagttataa ttaaaggaat caagataaaa acatctagcc aggtttttat
     3781 ctctaattga ctgacagtga aactcatcag agcataggaa gtagataagg ctagttttaa
     3841 agtctgaggg atagattgaa acaatttatt caaactaaaa aaggttgaca gaccaatcaa
     3901 tccaaatttt aagagagtgg tcagatagac agcatctggc atattcgtta gatcaaaaaa
     3961 gtaaactaga ggcgcgagaa aactacccaa gtaataacta gatagggcat agaagtttag
     4021 ccctagacca cttgtaaagg tgtaaaacag actactattt ccatgtagga tatttcgtaa
     4081 ggctacatca aaaataacgt attgatgaaa gccatctcct aatagaggag agttgtcgct
     4141 attccagtag ataccttgag atagatatac tccagacata atcactacag gaatgatgaa
     4201 agaaataaaa taggttcgat atgtttttaa aaatgatttc atgttacctc gtagaatgat
     4261 agaaaactca gttggttaac ccaactgagt tttgaagttt tatttagtct ttccaaagtt
     4321 ctttaacttt tgcttgtact tctgcatttt ctaggaattc atcgtaggtt tcatcgatac
     4381 ggtcaatgac gccattttta gataagacaa tgatatggtt agccaaagtt tgaataaact
     4441 cgtggtcatg gctggcaaag atgattgatt ctttaaagtt tttcaatcca tcattcaagc
     4501 ttgagataga ttccaagtcc aagtgatttg ttggatcatc aagtacaagg acatttgatt
     4561 ttaagagcat gagttttgaa agcatgacac gaactttttc tccccctgac aagacattta
     4621 caggtttgtt aacttcatct ccagagaaga gcatacggcc gaggaaacca cgtaggaaag
     4681 tattgtcatc atcttcttta cttgcgaatt gacgcaacca gtcaagaatt gattctcctc
     4741 ctgcaaaatc agctgagtta tcttttggta ggtaagattg actagttgta actccccact
     4801 ttacagttcc ttcatagtca atatctccca tgattgcacg aattaatgca gtcgtttgaa
     4861 tatcattttg tccaataagt gctgtcttat catctggacg caagatgaaa ctaatattat
     4921 ccaagatagt ttcaccatca atctttacag ttagattttc tactgtcaag agatcattac
     4981 caatctcacg ttccgcttta aagttgataa atggatattt acgactagat ggtacaatct
     5041 cttctagctc aatcttatca agcattttct tacgtgatgt tgcctgcctt gacttagaag
     5101 cattggcaga gaaacgagca acgaattctt gcaattgttt aattttttct tctgctttag
     5161 cattacggtc tgctagcaat ttagcagcaa gctcagaaga ttccttccag aagtcgtagt
     5221 ttccgacata gagtttgatt tttccaaagt caaggtcggc catgtgagta caaactttgt
     5281 ttaagaagtg acggtcgtgg gatactacga taactgtgtt atcaaagtca attaagaagt
     5341 cttctaacca agtaatcgat tggatatcca aaccgttagt aggctcgtcc aagagaagaa
     5401 catctggttt accaaaaagt gctttggcga ggagaacctt tactttttca ccgttggcca
     5461 attcgctcat gttttggtag tgtaattctt ctggaatgtt taggttttga agtagttgag
     5521 aggcttcact ctctgcttcc caacctccaa gttcggcaaa ctctccttcg agttcggcag
     5581 cacgaacccc gtcctcgtct gagaaatctt ccttcatgta gatagcatct ttctctttca
     5641 tgatgctata aagtttttca tttcccatga taacgacatc aatggcacgt tcatcttcgt
     5701 agtcaaagtg attttgacga agaacagaga gacgttcatc tggaccaaga gagatgtgac
     5761 cagtagtagg ttcgatatct ccagctaaaa tttttaaaaa ggttgatttt ccggcaccat
     5821 tagcaccgat taatccgtaa gtatttcctt ctgtaaattt gatattgaca tcatcaaaaa
     5881 gtttgcgatc actaaaacgt agtgaaacat cagatactgt aagcaatatt
//
LOCUS       DAVMEI010000104         5917 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVMEI010000104 DAVMEI010000000
VERSION     DAVMEI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.104"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            21..1871
                     /locus_tag="VYQ24_001515"
     CDS             21..1871
                     /locus_tag="VYQ24_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1968639.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(1957..2169)
                     /locus_tag="VYQ24_001516"
     CDS             complement(1957..2169)
                     /locus_tag="VYQ24_001516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968640.1"
                     /translation="MKIKEQTRKLAAGSSKHCFKVVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFEVVDRTDEVSNHIRQGDVDVV"
     gene            complement(2219..2851)
                     /locus_tag="VYQ24_001517"
     CDS             complement(2219..2851)
                     /locus_tag="VYQ24_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1968641.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(2873..3745)
                     /gene="sdaAA"
                     /locus_tag="VYQ24_001518"
     CDS             complement(2873..3745)
                     /gene="sdaAA"
                     /locus_tag="VYQ24_001518"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1968642.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(3754..4425)
                     /gene="sdaAB"
                     /locus_tag="VYQ24_001519"
     CDS             complement(3754..4425)
                     /gene="sdaAB"
                     /locus_tag="VYQ24_001519"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1968643.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            4667..5254
                     /locus_tag="VYQ24_001520"
     CDS             4667..5254
                     /locus_tag="VYQ24_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1968644.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(5307..5627)
                     /locus_tag="VYQ24_001521"
     CDS             complement(5307..5627)
                     /locus_tag="VYQ24_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1968645.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
BASE COUNT         1699 a         1158 c         1281 g         1779 t
ORIGIN      
        1 ttaaggagga gtagatttct atgaataaaa aactaacaga ttatgtgatt gatctggtgg
       61 aaattttaaa taaacaacaa aagcaggttt tctggggaat atttgatatt ttcagtatgg
      121 tggtttccat cattgtatct tatattttat tttatgggct gattaatcca gcacctgttg
      181 actacattat ctatacgagt ttggccttcc tgttctatca attgatgatt ggtttttggg
      241 ggttgaacgc gagcattagt cgttacagca agattacgga tttcatgaaa atcttttttg
      301 gtgtgactgc tagcagtgtc ttgtcatata gtatctgtta tgccttcttg ccactcttct
      361 ccatccgttt catcattctc tttatcttgt tgagtacctt cttgatttta ttgccacgga
      421 ttacttggca gttaatctac tccagacgca aaaaaggtag tggtgatgga gaacaccgtc
      481 ggaccttctt gattggtgcc ggtgatggtg gggctctttt tatggatagt taccaacatc
      541 caaccagtga attagaactg gtcggtattt tggataagga ttctaagaaa aagggtcaaa
      601 aacttggtgg tattcctgtt ttgggctctt atgacaatct gcctgaatta gccaaacgcc
      661 atcaaatcga gcgtgtcatc gttgcgattc cgtcgctgga tccgtcagaa tatgagcgta
      721 tcttgcagat gtgtaataag ctgggtgtca aatgttacaa gatgcctaag gttgaaactg
      781 ttgttcaggg ccttcaccaa gcaggtactg gcttccaaaa aattgatatt acggaccttt
      841 tgggtcgtca ggaaatccgt cttgacgaat cgcgtctggg tgcagaactg acaggtaaga
      901 ccatcttagt cacaggagct ggaggttcaa tcggttctga aatctgtcgt caagttagtc
      961 gcttcaatcc tgaacgcatt gtcttgctcg gtcatgggga aaactcaatc taccttgttt
     1021 atcatgaatt gattcgtaag ttccaaggga ttgattatgt acctgtgatt gcggacattc
     1081 aagactatga tcgtttgttg caagtctttg agcagtacaa acctgctatt gtttatcatg
     1141 cggcagccca caagcatgtt cctatgatgg agcgcaatcc aaaagaagcc ttcaaaaaca
     1201 atatccgtgg aacttacaat gttgctaagg ctgttgatga agctaaagtg tctaagatgg
     1261 ttatgatttc gacagataag gcagtcaatc caccaaatgt tatgggagca accaagcgcg
     1321 tggcggagtt gattgtcact ggctttaacc aacgtagcca atcaacctac tgtgcagttc
     1381 gttttgggaa tgttcttggt agccgtggta gtgtcattcc agtctttgaa cgtcagattg
     1441 ctgaaggtgg gcctgtaacg gtgacagact tccgcatgac ccgttacttt atgaccattc
     1501 cagaagctag ccgtctggtt atccatgctg gtgcttatgc caaagatggg gaagtcttta
     1561 tccttgatat gggcaaacca gtcaagattt atgacttggc caagaagatg gtgcttctaa
     1621 gtggccacac tgaaagtgaa attccaatcg ttgaagttgg aatccgccca ggtgaaaaac
     1681 tctacgaaga actcttggta tcaaccgaac tcgttgataa tcaagttatg gataagattt
     1741 tcgttggtaa ggttaatgtc atgcctttag aatccatcaa tcaaaagatt ggagagttcc
     1801 gcactctcag tggagatgag ttgaagcaag ctattatcgc ctttgctaat caaacaaccc
     1861 acattgaata aaaaagaaaa acgcatagta tcaagttaca caaccttggt aatatgcgtt
     1921 ttattatgta gagacttata ctcttcgaaa atctcttcaa accacgtcaa cgtcgccttg
     1981 ccgtatatgg ttactgactt cgtcagttct atccacaacc tcaaaacagt gttttgagct
     2041 gacttcgtca gttccatcca caaccttaaa acagtgtttt gagctgactt cgtcagttcc
     2101 atctacaacc ttaaaacagt gttttgagct gcccgcagct agtttcctag tttgctcttt
     2161 gattttcatt gagtattact tcattttctt ctgaaatgga attgttaccc agtctatgct
     2221 attgaaaata cgccaaaact tctaagggtt tgtgagcgat ataatcaggt tgatagttta
     2281 gtagatctgc ttgctctcca aatccccaag tgatggccaa tttctgaata cctgtttctc
     2341 gagctcccag catatcaaac ttggtatctc cgatgatgat ggcttgttct ggtgctagtt
     2401 gatgtgtctg caaggcttgg tgaatgacat ctgccttatg gggtgcttca gggctagaac
     2461 cataaatgcc atcaaagaaa tgatggattt ccaagttttt tgccatgtct tgagcagtag
     2521 atgtatcctt tgtcgtggtg atgtagagtg gataactgct cgataactcc tcaagcaagt
     2581 ctataatctg aggaaagagt tgagcttcat agatgccttt tgccttatag taagaacgat
     2641 atatctgcac ggcttcagaa atttggtctt tggacaggca ggtcgcaaaa ctactttcga
     2701 gaggtggtcc cataaaacca cgaatagttt tggcatcagg gctaggcacc cccagctctt
     2761 taaaggtata ggtaaaggca ttgtgaatcc cgatagaact atcaacgagg gttccatcca
     2821 aatcgaaaaa aatcgctgtg atagaggtca tggtttctcc tatttgataa gcttattctc
     2881 cgaaaatttc tttttggagg cgacgaccag taggggtggt agcgagtcca ccttcagctg
     2941 tttcacgaaa ggcagttggc atgcttgctc ctacttggta catggcatcg atcacttcat
     3001 ccacagggat tttagattcg atacctgcca aggccatgtc tgctgcgatg aaagcaaagc
     3061 tagctcccat ggcattacgt ttgacacagg gaacttcgac caaacctgca acagggtcac
     3121 agatgaggcc tagcatattt ttaatgacaa aggcaatagc ttgactggcc tgataaggtg
     3181 ttccacctgc agccagagtc aaggcggcag cactcatagc agaggctgaa ccaacttcag
     3241 cttgacaccc accctcagca cctgagatgg aggcattgtt tgcgatgact agtccaaagg
     3301 caccagcagc aaagaggaaa tccaattgtt gctcgtggct gaggtctaat ttttcaatag
     3361 cagcagtgag aacggatggc agacagccag cacttccagc ggttggagtg gcacagacca
     3421 agcccatttt ggcattgtgt tcattgactg cgatggcatt tcgggcagcc gagagaatcg
     3481 tataatctga cagagttttt ccgttttcga tgtagtgatc caatttggca gcatctccac
     3541 ctgtcaggcc actacgagat ttattttcat tgaggccaag ttggacagag gctttcataa
     3601 cttccagatt gcgttccatg agaaggaaga cttcttcacg ttcgcgaccg gtcaattcaa
     3661 actctgttgt aatcatgagt tctgcgacat ttccttgaaa gtccagatct gcttgctcga
     3721 ccaattcttt gatagaataa aacatgcttc ctcctattta aagaaattga cattgtggag
     3781 atgagggatt tttcgaattt cttcgatagc ctcatcacag ttgcgactgt caacttcgat
     3841 aatcataatg gctttttcac cagctttttc acgagtgaca ttcatctggg cgatattgat
     3901 accatagcgg gaaagcgcct ctgtaacaag ggcaatcata cctggaatat cttgatgaac
     3961 gatgatgata gtcggtgtat tcatattgag agagacggca aaaccattga gttcggttac
     4021 ctgaatattt cctccaccga tagaaatacc agtcacgctg atggtcttgt gggcattttt
     4081 aacagtaatt ttagtggtgt tagggtgagg ggcattgctg tctttctgaa tggtccagac
     4141 aatcttgata ccacgcttgt gggcaatttc cagactattt ggaatttcag gatcatctgt
     4201 atccattcct aaaatacctg caacaagggc taggtctgtt ccgtgaccac gataggtctt
     4261 ggcaaatgag ttaaaaagtt ggaattcaac ttctgtcgga gtatcatcaa aaatggaaga
     4321 gacaatcttc ccaatacgaa cagcaccagc ggtatggcta ctagatgggc caatcataac
     4381 tggtccgatg atatcaaaga cagattgaaa acgaagtgat ttcatcagtt tccccttata
     4441 aaaattctta tctctattat atcaaagaat gaggggcttg gctttaattg tggatgaaaa
     4501 cctttctaat acctcaaata gcataaaaat agtatctttt atgacaaaaa acaccttatt
     4561 tagggaaata aaaaataatt ttgtaatatt tctacataaa agtgtcaaga aacggtaata
     4621 tttaaagggt atgatagaac tatagaaaga aggagaattt tcgaatatga aatcaataac
     4681 taaaaagatt aaagcaactc ttgcaggagt agctgccttg tttgcagtat ttgctccatc
     4741 atttgtatct gctcaagaat catcaactta cactgttaaa gaaggtgata cactttcaga
     4801 aatcgctgaa actcacaaca caacagttga aaaattggca gaaaacaacc acattgataa
     4861 cattcatttg atttatgttg atcaagagtt ggttatcgat ggccctgtag cgcctgttgc
     4921 aacaccagcg ccagctactt atgcggcacc agccgctcaa gatgaaactg tttcagctcc
     4981 agtagcagaa actccagtag taagtgaaac agttgtttca actgtaagcg gatctgaagc
     5041 agaagccaaa gaatggatcg ctcaaaaaga atcaggtggt agctatacag ctacaaatgg
     5101 acgttatatc ggacgttacc aattaacaga ttcatacctg aacggtgact actcagctga
     5161 aaaccaagaa cgtgtagcag atgcctacgt tgcaggacgt tacggttcat ggactgctgc
     5221 taaaaacttc tggcttaaca atggctggta ttaagaacta acgaacaaat aatatgtaaa
     5281 gagtcgagga aatccctcga ctttttttat aatttctttg gttgataatg ggtctctact
     5341 tcttttttga gatgagacag catagaagtt tcctcggtca gctctaggag tgagaaccct
     5401 ttttggataa gtttcgcatc atccctacaa atcccaatac ggacatagca gatagccagc
     5461 ctatcgtagc ttttcttatt cttggcgtcc tccagtaaag tgtagcagat ttgcttacac
     5521 atgtttttat cttgattgta tagatagagt gtggaaaggt ttaatagaat tgtcattcgc
     5581 aagtcatata aatgttgata gtttttatag acttctaagc gttgcaagat tttctcggat
     5641 tattgacaaa taaacggaca ttctgataga cgattgccct agcggcttag tgtaaacttt
     5701 acaaatttgg cttgtaattt ttttataaaa ttcacttcag ttacttgaca gttattatat
     5761 tttcgcttat gcttattata tagataaaag gacgggatag gagtttaaaa atttgaaagc
     5821 gctatccata atcataacgc ataattttct ccttttcgtg aaaaaagtta acgtaatttt
     5881 gaaatgagct taataagagg tactatgtag gtagaaa
//
LOCUS       DAVMEI010000105         5835 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVMEI010000105 DAVMEI010000000
VERSION     DAVMEI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.105"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..1727
                     /locus_tag="VYQ24_001522"
     CDS             27..1727
                     /locus_tag="VYQ24_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1968646.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            1720..2196
                     /gene="ilvN"
                     /locus_tag="VYQ24_001523"
     CDS             1720..2196
                     /gene="ilvN"
                     /locus_tag="VYQ24_001523"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1968647.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            2262..3284
                     /gene="ilvC"
                     /locus_tag="VYQ24_001524"
     CDS             2262..3284
                     /gene="ilvC"
                     /locus_tag="VYQ24_001524"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1968648.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            3391..3627
                     /locus_tag="VYQ24_001525"
     CDS             3391..3627
                     /locus_tag="VYQ24_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968649.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            3865..4221
                     /locus_tag="VYQ24_001526"
     CDS             3865..4221
                     /locus_tag="VYQ24_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1968650.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEIFGDE"
     gene            4271..5521
                     /gene="ilvA"
                     /locus_tag="VYQ24_001527"
     CDS             4271..5521
                     /gene="ilvA"
                     /locus_tag="VYQ24_001527"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1968651.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
BASE COUNT         1786 a          994 c         1341 g         1714 t
ORIGIN      
        1 tttttaagag gaaagaaagg ggaataatgg agaaaatcag tttagaatct cctaagacgg
       61 ggtcggacct agttttggaa acacttcgtg atttaggagt tgataccatc tttggttatc
      121 ctggtggtgc ggttttgcct ttttatgatg cgatatataa ttttaaaggc attcgccaca
      181 ttctagggcg ccatgagcaa ggttgtttgc atgaagctga aggctatgcc aaatcaactg
      241 gaaagttggg tgttgccgtc gtcactagtg gaccaggagc aacaaatgcc attacaggga
      301 ttgcggatgc tatgagcgat agcgttcccc ttttggtctt tacaggtcag gtggcgcgag
      361 cagggattgg gaaggatgcc tttcaggagg cagacatcgt aggaattacc atgccaatca
      421 ctaagtacaa ttaccaagtt cgtgagacag ctgatattcc gcgtatcatt acggaagctg
      481 tccatatcgc aactacaggc cgtccagggc cagttgtaat tgacctacca aaagacatat
      541 ctgctttaga aacagacttc atttattcac cagaagtgaa tttaccaagt tatcagccga
      601 ctcttgagcc gaatgatatg caaatcaaga aaatcttgaa gcaattgtcc aaggctaaaa
      661 agccagtctt gttagctggt ggtggaatta gttatgctga ggctgctacg gaactaaatg
      721 aatttgcaga acgctatcaa attccagtgg taaccagtct tttgggacaa ggaacgattg
      781 caacgagtca cccactcttt cttggaatgg gaggcatgca cgggtcattc gcagcaaata
      841 ttgccatgac ggaagcggac tttatgatta gtattggttc tcgtttcgat gaccgtttga
      901 cggggaatcc taagactttc gctaagaatg ctaaggttgc ccacattgat attgacccag
      961 ctgagattgg caagattatc agtgcagaca ttcctgtagt tggagatgct aagaaggcct
     1021 tgcaaatgtt gctagcagaa ccaacagttc acaacaatac tgaaaagtgg attgagaaag
     1081 tcactaaaga caagaatcgt gttcgttcgt atgacaagaa agagcgtgtg gttcaaccgc
     1141 aagcagttat tgaacgaatt ggtgaattga cgaatggaga tgccattgtg gtaacagacg
     1201 ttggtcaaca ccaaatgtgg acagctcagt attatcccta ccaaaatgaa cgtcagttag
     1261 tgacttcagg tggtttggga acaatgggct ttggaattcc agcagcaatc ggtgctaaaa
     1321 ttgctaaccc agataaggaa gtagtcttgt ttgttgggga tggtggtttc caaatgacca
     1381 accaggagtt ggctattttg aatatttaca aggtgccaat caaggtggtt atgctgaaca
     1441 atcattcact cggaatggtt cgccagtggc aggaatcctt ctatgaaggc agaacatcag
     1501 agtcggtctt tgataccctt cctgatttcc aattgatggc gcaggcttat ggtattaaaa
     1561 actataagtt tgacaatcct gagaccttgg ctcaagacct tgaagtcatc actgaggatg
     1621 ttcctatgct aattgaggta gatatttctc gtaaggaaca ggtgttacca atggtaccgg
     1681 ctggtaagag taatcatgag atgttggggg tgcagttcca tgcgtagaat gttaacagca
     1741 aaactacaaa atcgttcagg agtcctcaat cgctttacag gtgtcctatc tcgtcgtcag
     1801 gttaatattg aaagcatctc tgttggagca acagaagatc cgaatgtatc gcgtatcact
     1861 attattattg atgttgcttc tcatgatgaa gtggagcaaa tcatcaaaca gctcaatcgt
     1921 cagattgatg tgattcgcat tcgagatatt acagacaagc ctcatttgga gcgcgaggtg
     1981 attttggtta agatgtcagc gccagctgag aagagagctg agattttagc gattattcaa
     2041 cctttccgtg caacagtggt agacgtagcg ccaagctcga ttaccattca gatgacggga
     2101 aatgcagaaa agagcgaagc cctattgcga gtcattcgcc catacggtat tcgcaatatt
     2161 gctcgaacgg gtgcaactgg atttacccgc gattaaaaat ccaacttaaa tttgttaaac
     2221 cagcttaaaa ggcaataaat aatagaaaag agagaaaagc tatgacagtt caaatggaat
     2281 atgaaaaaga tgttaaagta gcagcacttg acggtaaaaa aatcgccgtt atcggttatg
     2341 gttcacaagg gcatgcgcat gctcaaaact tgcgtgattc aggtcgtgac gttattatcg
     2401 gtgtacgtcc aggtaaatct tttgataaag caaaagaaga tggatttgat acttacacag
     2461 tagcagaagc tactaagttg gctgatgtta tcatgatctt ggcgccagac gaaattcaac
     2521 aagaattgta cgaagcagaa atcgctccaa acttggaagc tggaaacgca gttggatttg
     2581 cccatggttt caacatccac tttgaattta tcaaagttcc tgcggatgta gatgtcttca
     2641 tgtgtgctcc taaaggacca ggacacttgg tacgtcgtac ttacgaagaa ggatttggtg
     2701 ttccagctct ttatgcagta taccaagatg caacaggaaa tgctaaaaac attgctatgg
     2761 actggtgtaa aggtgttgga gcggctcgtg taggtcttct tgaaacaact tacaaagaag
     2821 aaactgaaga agatttgttt ggtgaacaag ctgtactttg tggtggtttg actgccctta
     2881 tcgaagcagg tttcgaagtc ttgacagaag caggttacgc tccagaattg gcttactttg
     2941 aagttcttca cgaaatgaaa ttgatcgttg acttgatcta cgaaggtgga ttcaagaaaa
     3001 tgcgtcaatc tatttcaaac actgctgaat acggtgacta tgtatcaggt ccacgtgtaa
     3061 tcactgaaca agttaaagaa aatatgaagg ctgtcttggc agacatccaa aatggtaaat
     3121 ttgcaaatga ctttgtaaat gactataaag ctggacgtcc aaaattgact gcttaccgtg
     3181 aacaagcagc taaccttgaa attgaaaaag ttggtgcaga attgcgtaaa gcaatgccat
     3241 tcgttggtaa aaacgacgat gatgcattca aaatctataa ctaattagaa atatatagcg
     3301 ctggagatga ttttatgaaa aagattatga gaaaaattgc atcgttatta ttggttctag
     3361 ttgtataatg taattacacc gtcggtaata gtgctagcag accaaaataa agcagattgg
     3421 tcgtatgatg aaaatgctgt aattaacatt tatgatgatg ctaattttga agatggtagg
     3481 ttgcatatga cctttgaaca attcttcaaa ttggcacaaa tagctagaga agaaggtctt
     3541 gaaattcatt ctccgtttga gagagctggt gcgactaaat ctgctcgtta tatagcgaaa
     3601 tggattttga gaaataaaaa atattaacaa atatagttgg taaatcatta ggacctaaat
     3661 cagctgttag attcggagaa gctttatcct atattgaagg tcctcttcgc agaataaatg
     3721 agacgataga tggcggttta tatcaaatag agcaaattat tgcatctgga ttgaaagaat
     3781 cgggtttaaa tgactggact gcgaaaactt tagcttcagc tattcgtggg atattagatg
     3841 tacttattta ggggttgaaa tcatatgaat attaccaatt tgttttctat caagacagga
     3901 tgtgatgaaa ctgataggca actgcaaaaa ctattttttc agttggattt acaattggga
     3961 gaattgacag atcaactaag aaaattagat tctaattttg ttcctcgtag tcaatttgta
     4021 gacacgttgg atttgaatga tgtagaatat aaagaaattt taaactattt tatcttccat
     4081 cgtaatgata gtgaagaaag tttggtagaa tggttatatg attggatttc cacaaatcgt
     4141 tatgaacttc ctaaagagtt ttcgattcgt atggctcata aataccatga aagtgttact
     4201 gaaattttcg gagatgaata actaaaaaac agtcattagt gactgttttt tatagaaaaa
     4261 gaggttttat atgttaagtt caaaagatat aatcaaggct cacaaggtct tgaacggtgt
     4321 ggttgtgaat actccactgg attacgatca ttatttatcg gagaagtatg gtgctaagat
     4381 ttatttgaaa aaagaaaatg cccagcgtgt tcgctccttt aaaattcgtg gtgcctatta
     4441 tgccatttcc cagctcagca aggaagaacg tgaacgtggg gtagtttgcg cttctgcggg
     4501 aaatcatgcg cagggagtag cctatacttg taatgaaatg aaaattcctg ctactatctt
     4561 tatgcccatt actacgccac aacaaaagat tggtcaggtt cgcttttttg gtggggattt
     4621 tgtaactatt aaactagttg gagatacctt tgatgcctca gccaaagcag ctcaagaatt
     4681 tacagtctct gaaaatcgta cctttattga tccttttgat gatgctcatg ttcaagcagg
     4741 tcaaggaaca gttgcttatg agattttaga agaagctcga aaagaatcga ttgattttga
     4801 tgctgtcttg gttcctgttg gtggtggcgg tctcattgcc ggggtttcta cctatatcaa
     4861 ggaaacaagt ccagagattg aggttatcgg agtagaggcg aatggagcgc gttccatgaa
     4921 agctgccttt gaggctggag gtccagtcaa actcaaggaa attgataaat ttgcggatgg
     4981 gattgctgtg caaaaggtag gtcagttgac ctatgaagca actcgtcaac atattcaaac
     5041 tttggtaggt gtcgatgagg gattgatttc tgaaaccttg attgaccttt actctaagca
     5101 agggatagtc gcagaacctg ctggagcagc tagtatcgcc tctttagagg ttttagctga
     5161 atatattaag gggaaaacca tttgttgtat catttctgga ggaaataatg atatcaaccg
     5221 tatgccagaa atggaagagc gtgccttgat ttatgatggt atcaaacatt attttgtggt
     5281 caatttccca caacgtccag gagctttgcg tgagtttgta aatgatatcc tggggccaaa
     5341 tgatgatatc acacgttttg agtatatcaa acgagctagc aagggaacag gcccagtatt
     5401 gattgggatc gctttagcag ataagcatga ttatgcaggt ttgattcgta gaatggaagg
     5461 ttttgatcca gcttatatta acttaaatgg taatgaaacg ctttataata tgcttgtctg
     5521 aggactaata aaaaaatatc atattatttg tacaactgat gtatatatgc tataatgaag
     5581 atgaagaaag gggacgattc ctatggtttt agaaaactta tcgtgcaatt cttacttctg
     5641 ttcattatag cacttatgat gtttggctca gatttccatc cttcattttg atttcctatc
     5701 tattgacaag catagtcaca ctgtctttaa tactcttcga aaatctcttc aaaccacgtt
     5761 agctctatct gcaacctcaa aacagtgttt tgagcaactt gcggctagct tcctagtttg
     5821 ctctttgatt ttcat
//
LOCUS       DAVMEI010000106         5823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVMEI010000106 DAVMEI010000000
VERSION     DAVMEI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.106"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1058
                     /locus_tag="VYQ24_001528"
     CDS             <1..1058
                     /locus_tag="VYQ24_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968652.1"
                     /translation="SMIITNLRTLYVYVLQMTNRLKDSSIILLSDRVLYVLLLFLFIV
                     FGWHEYKVMILADILGRSFSLILSFWICKDIVFQPLSKFIFNIKESFDNIRVGINLML
                     SNIASSLIIGIVRLGIQWNWNIETFGKVSLTLSVSNLLMTFINAIGLVVFPLLRRTKA
                     ENLPKIYSNLRNVLMLIMFAILLIYYPLKIVLDLWLPAYQDALIFMTLIFPMSVYEGK
                     MALVINTYLKALRMERDILRVNALVMLISMGVTLVTTYLLNSLELTVVSIVVLLALRS
                     IIAELILSKKLDVSVKKDIVLEFLLTLVFISSSWYLPIGLAVIVYTIAYGLYLYLKHE
                     DIKTYLAYFKASKKTSN"
     gene            1123..2211
                     /gene="wecB"
                     /locus_tag="VYQ24_001529"
     CDS             1123..2211
                     /gene="wecB"
                     /locus_tag="VYQ24_001529"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEV1968653.1"
                     /translation="MKKIMLVFGTRPEAIKMCPLVNELKKHEDMETIVCVTGQHKEMV
                     SPVLDLFGVVPDYDLEIMKANQTLFSITTSILEKIKPVLEKEQPDIVLVHGDTTTTYA
                     AALAAFYLGIKVGHVEAGLRTYNLQSPFPEEFNRQSTSIIATYHFAPTELAKENLLKE
                     GRENVYVTGNTVIDALTTTVQEDYTHTHLDLNANNRLILLTAHRRENLGEPMRHMFRA
                     VKRVLNEYEDVKVIYPIHKNPLVRETAAEIFGDTERIQIIEPLDVLDFHNFMNQSYMI
                     LTDSGGVQEEAPSLGKPVLVMRDTTERPEGVAAGTLKLVGTDEETIYQNFKLLLDDSG
                     EYKKMSQASNPYGNGDASQQIVQILRGI"
     gene            2374..3243
                     /gene="rfbA"
                     /locus_tag="VYQ24_001530"
     CDS             2374..3243
                     /gene="rfbA"
                     /locus_tag="VYQ24_001530"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1968654.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFKDLLLDGSEFGIKLSYAEQPSPDGLAQAFLIGEEFIGDDSV
                     ALILGDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKQIKPSARGELEITDVNKAYLNRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEISYRMGYISREDVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            3244..3837
                     /locus_tag="VYQ24_001531"
     CDS             3244..3837
                     /locus_tag="VYQ24_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1968655.1"
                     /translation="MSDNFFGKTLVVRKIDAIPGLLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADDGKVLGSWVDLREG
                     ETFGNVYQTEIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPALGIEWENLAEAEVSEADKHHPLLRDVKPLTKDEL"
     gene            3849..4895
                     /gene="rfbB"
                     /locus_tag="VYQ24_001532"
     CDS             3849..4895
                     /gene="rfbB"
                     /locus_tag="VYQ24_001532"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1968656.1"
                     /translation="MTEYKKIIVTGGAGFIGSNFVHYVYNNFPDVHVTVLDKLTYAGN
                     RANIEEILGDRVELVVGDIADAALVDKLAAEADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGLGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQPAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKETIKWYTDNQDWWKSEKE
                     AVEANYAKTQQVIK"
     gene            complement(4957..5811)
                     /gene="rfbD"
                     /locus_tag="VYQ24_001533"
     CDS             complement(4957..5811)
                     /gene="rfbD"
                     /locus_tag="VYQ24_001533"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1968657.1"
                     /translation="MILITGANGQLGTELRYLLDERNVDYVAVDVTEMDITNSEMVEK
                     VFAEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKASEKYGAILVYISTD
                     YVFDGKKPVGQEWEVDDIPDPQTEYGRTKRMGEELVENLTSQHYIIRTAWVFGNYGKN
                     FVFTMQSLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYVTENQKEYGYYHLSNDAAEDT
                     TWYDFASEILKDSDVEVVPVDSSKFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALKE
                     FYKQEVRK"
BASE COUNT         1713 a         1090 c         1244 g         1776 t
ORIGIN      
        1 taagtatgat aatcacaaat ttaagaacgc tctacgtgta tgttttgcag atgacaaacc
       61 gcttgaagga tagttccatc attctactca gtgatcgcgt tttatatgtg ctccttttat
      121 tcctgtttat tgtatttggt tggcatgaat acaaggttat gattttggca gatattctag
      181 gtcgatcatt ttctctcata ctttccttct ggatttgtaa agatattgtg tttcagcctt
      241 tgtcaaaatt catctttaat ataaaagagt cttttgataa tatccgagtt ggtatcaatt
      301 taatgttatc caacatcgca agtagtttga ttatcggtat tgttcgtcta ggaatccaat
      361 ggaactggaa tatcgaaaca ttcgggaaag tatctctgac cttgagtgtt tccaatctat
      421 tgatgacttt cattaatgcc attggactag tcgtctttcc gctattaaga cgaacaaaag
      481 cagaaaactt acctaagatt tattctaatt taagaaacgt tttgatgctt atcatgttcg
      541 cgattttgct catttactat cctttaaaaa ttgtattaga cctctggttg ccagcctatc
      601 aagatgcctt gattttcatg acccttattt tccctatgtc agtctatgaa gggaaaatgg
      661 cattggtcat taatacttac ttaaaggcat taagaatgga aagagatatc cttagagtca
      721 atgccttagt aatgttgatc agtatgggag tgaccctggt tacaacatac ctgttaaata
      781 gtttggagct gactgttgta tcgatagttg ttttgctagc tttgagaagt atcatagccg
      841 aattaattct gtctaaaaaa ctggatgttt cggttaagaa ggatattgta ttagaatttc
      901 ttttgacgct tgtctttatt tcttcaagtt ggtatttgcc gattgggctg gcagtaattg
      961 tctacacaat agcctacggt ttatatctct atttgaaaca cgaagatatc aaaacctatt
     1021 tagcttactt taaagctagt aaaaaaacat caaattaaaa aattatataa tgattaagtg
     1081 gtagattcta tttctaccgt tttagatatt cgggaggtaa tgatgaaaaa aattatgtta
     1141 gtattcggta cacgtccgga agccatcaaa atgtgtccat tagtcaatga gttgaaaaaa
     1201 cacgaagata tggaaacaat tgtgtgtgtt actggacaac acaaagagat ggttagtcct
     1261 gttttagatt tatttggtgt tgtaccagat tatgatttag aaattatgaa ggctaaccaa
     1321 accttgttct ctatcacaac tagtatcttg gaaaagataa aaccagtttt agagaaggaa
     1381 caaccagata ttgtcctagt tcacggtgac actacgacaa cttatgcagc agccttggca
     1441 gcattctatt tgggaattaa agtaggacat gttgaagctg gtttgcgaac gtacaattta
     1501 caaagtccat ttcctgaaga atttaacagg caatcgacat caatcattgc aacttaccat
     1561 tttgctccaa ctgagttggc taaagaaaat ctcttaaaag aaggtagaga gaatgtttat
     1621 gtgactggaa atactgtcat tgatgctctt acaactactg ttcaagagga ttatacacac
     1681 actcatttag atttaaacgc taacaatcgt ctcatcttat tgactgctca tagacgcgaa
     1741 aatctcggcg aaccgatgag acatatgttt agagcagtta aacgagtatt gaatgaatac
     1801 gaagatgtta aagtcattta tccaattcat aagaatcctt tggtacgtga aacagctgca
     1861 gagatctttg gagatacaga acggattcaa attattgaac ctttggatgt tcttgatttc
     1921 cacaacttca tgaaccaaag ttatatgatt ttaacagatt ctggcggagt tcaggaagaa
     1981 gcaccttctt taggaaaacc tgtattggtt atgcgcgata ctactgaaag acctgaagga
     2041 gtagcggcag gaaccttgaa attagtagga actgacgaag aaacaattta tcaaaacttc
     2101 aaactacttc tagatgattc tggagaatac aaaaaaatga gtcaggctag caatccatac
     2161 ggtaatggtg atgctagtca acagattgtt cagattttgc gtgggattta agaagattct
     2221 ctaaaagagt ctaagaggaa tccactccac aaacataaaa ctcttatgct atcatttatg
     2281 attgagatag actcataact taatattttt atcctaagaa aattcgttca tattcaatat
     2341 gaatgaaaaa caacgaagaa aggttatttc attatgaaag gtattattct tgcaggcggc
     2401 tcaggtaccc gcctgtaccc acttactcgg gctgcgtcaa aacagctgat gccggtttat
     2461 gataaaccta tgatttatta tccgttgtcg acattaatgt tggctggaat taaagatatt
     2521 ttgattatct caactcctca agatttgccc cgttttaagg acttgctctt ggatggttcc
     2581 gaatttggga tcaagctttc ctatgcggaa caacctagtc ccgatggact tgctcaggct
     2641 tttcttatcg gtgaagaatt tatcggtgac gatagcgttg ccttgatttt aggcgacaat
     2701 atctaccatg gtcctggtct gagcacaatg cttcaaaaag cagccaagaa agagaaaggt
     2761 gcgactgttt ttggctacca agtgaaggat ccagagcgtt ttggtgtggt tgagtttgat
     2821 acagacatga atgctatttc tatagaagaa aaaccggagt atcctcgctc caactatgca
     2881 gtgacaggac tgtatttcta tgataatgat gttgtggaga ttgctaaaca gatcaaacct
     2941 agtgctcgtg gcgagttaga aattacagac gttaacaagg cttacctaaa tcgtggtgac
     3001 ctttctgttg agctgatggg gcgtggtttt gcctggttgg atacgggaac ccatgaaagc
     3061 ttgctagaag cttctcagta tattgaaacg gttcagcgta tgcagaacgt tcaagtggca
     3121 aatctagaag aaatttccta tcgtatgggc tacattagtc gtgaagacgt gctggaattg
     3181 gctcagcctc ttaagaaaaa tgaatacgga caatatttgc tccgtttgat tggagaagca
     3241 tagatgtcag ataatttttt tggaaagaca cttgtggtac gcaagattga tgctatacca
     3301 ggactgctag agtttgacat tcccgttcat ggagacaatc gtggttggtt taaggaaaat
     3361 ttccagaagg aaaagatgct accgcttggt tttcctgaaa gcttctttgc tgcagggaaa
     3421 ctgcaaaata acgtcagctt ttctcgcaaa aatgttcttc gaggactcca tgctgaacct
     3481 tgggacaagt atatctctgt tgcagacgat gggaaggttt taggatcttg ggtagatttg
     3541 cgtgaaggcg agacctttgg aaatgtttac cagacagaga ttgatgctag caagggaatt
     3601 tttgtccctc gtggcgtagc caacggtttt caagtcctct cagatacagt ttcctatagc
     3661 tatctggtca atgactactg ggctcttgaa ctcaaaccca aatatgcctt tgttaactat
     3721 gctgacccag cattgggaat tgagtgggaa aacctagcgg aagctgaggt ttcagaggca
     3781 gacaaacacc atcctctatt aagggatgtc aaaccactta cgaaagacga gttgtaaaag
     3841 gaaacattat gactgaatac aaaaaaatta tcgtgacagg tggagctggt tttatcggtt
     3901 ctaactttgt ccactatgtt tacaataact ttccagatgt ccatgtgaca gtgctggaca
     3961 agctgactta tgcaggtaat cgtgccaata tcgaggaaat tttaggcgac cgtgttgagt
     4021 tggttgttgg agatattgct gatgcagcct tggtagacaa gttggcggct gaagcggatg
     4081 ctatcgttca ctatgcggca gaaagccaca atgataactc gctcaatgac ccgagtccat
     4141 ttatccacac caactttatc ggaacttaca cacttttaga agcggctcgt aaatacgaca
     4201 ttcgtttcca ccatgtatcg actgatgaag tctatggtga cctgcctctg cgtgaagatt
     4261 tgccaggtca tggggaaggc ctaggtgaga aatttaccgc tgaaaccaag tacaatccaa
     4321 gctcgcctta ctcatcaacc aaggctgctt cagacttgat cgttaaagct tgggtgcgct
     4381 catttggtgt taaagcaacg atttctaact gttcaaacaa ctatggtcca taccagcata
     4441 tcgagaagtt cattccgcgc cagattacga atatcttgag cggtatcaag ccaaaactct
     4501 acggagaagg taagaatgtg cgtgactgga ttcacaccaa tgaccattca tcaggcgttt
     4561 ggacgattct gaccaagggt caaatcggtg aaacttactt gatcggtgct gacggtgaga
     4621 agaacaacaa ggaagtgtta gagctcattc tcaaggagat ggggcaaccc gctgacgctt
     4681 atgaccatgt gaccgatcga gctggtcacg acttgcgtta tgcgattgat gctagcaagc
     4741 tccgtgatga gttggggtgg aagccagagt ttaccaactt tgaagcaggc ctcaaagaga
     4801 ccatcaagtg gtacacagat aaccaagact ggtggaaatc tgaaaaagaa gcagtagagg
     4861 ctaactatgc taaaacgcaa caagtgatta aataataaga tactaaaaaa gcaagagacc
     4921 tcgaggtctc ttgcttttct agtatgtagt aagcttttat tttcttactt cttgtttata
     4981 aaactcttta agggcatctt gccaggttgg gatgacgaaa cctgttgcct ttgcctttgc
     5041 caaactcata gttgagttga gaggtcgttt agccttagcg gggaacttgc tagagtctac
     5101 tggcactact tcaacatcac tgtccttaag aatctcgcta gcaaagtcat accaagtggt
     5161 gtcttcagct gcgtcatttg ataagtgata gtagccatac tctttttgat tttcagtcac
     5221 ataggtcatg aattcggcca aggtccgtgt ccaagttggg cgaccgtatt ggtcgttgac
     5281 cactgtgagc gttttatggg ttttggctag gctttgcatg gtaaagacaa agttttttcc
     5341 ataatttcca aaaacccaag cagtacgaat gatgtaatgc tgtgacgtaa ggttttcaac
     5401 aagttcttct cccattcgct tggtacgtcc gtactctgtt tgcggatcag gtatgtcatc
     5461 gacttcccac tcttgtccta ctggtttctt tccatcaaag acgtagtctg ttgagatata
     5521 gaccagaata gctccgtatt tctcagaggc cttggctaca ttttcagttc cagttacgtt
     5581 gatggcaaaa tccagttctt tcccttcatc ttcggctgca tcaacagcag tgtaggctgc
     5641 acaatgatag actagagttg gcttaacctc agcaaagact ttttcaacca tttcagaatt
     5701 agtgatatcc atttcggtca catccactgc aacatagtcc acatttcgct catctagtaa
     5761 ataacgtagt tcggtaccaa gttgaccatt tgcacctgta attaagatca tatttttctc
     5821 ctt
//
LOCUS       DAVMEI010000107         5746 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVMEI010000107 DAVMEI010000000
VERSION     DAVMEI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.107"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(227..1126)
                     /locus_tag="VYQ24_001534"
     CDS             complement(227..1126)
                     /locus_tag="VYQ24_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968658.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(1198..1758)
                     /gene="rnmV"
                     /locus_tag="VYQ24_001535"
     CDS             complement(1198..1758)
                     /gene="rnmV"
                     /locus_tag="VYQ24_001535"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1968659.1"
                     /translation="MKEKISQVIVVEGRYDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1758..2531)
                     /locus_tag="VYQ24_001536"
     CDS             complement(1758..2531)
                     /locus_tag="VYQ24_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1968660.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMIISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2836..3501)
                     /locus_tag="VYQ24_001537"
     CDS             complement(2836..3501)
                     /locus_tag="VYQ24_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEV1968661.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLETTPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKNKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRLVAPNAA
                     PKTTSAPQATAGTMQNVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            complement(3764..3898)
                     /gene="rpmH"
                     /locus_tag="VYQ24_001538"
     CDS             complement(3764..3898)
                     /gene="rpmH"
                     /locus_tag="VYQ24_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1968662.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(4043..5257)
                     /locus_tag="VYQ24_001539"
     CDS             complement(4043..5257)
                     /locus_tag="VYQ24_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1968663.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5413..5706)
                     /locus_tag="VYQ24_001540"
     CDS             complement(5413..5706)
                     /locus_tag="VYQ24_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968664.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVVKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
BASE COUNT         1642 a         1324 c          998 g         1782 t
ORIGIN      
        1 ttctaaaatt atttgttttt ctctaaattg tcaaaatagc accctgaaat gaacttttat
       61 agtcattata ctccatcttt atcacactat caaggacatc aatgtcctta aaatctaact
      121 atttcatatt atttttagct tatatctaaa aaaacagttc taaagacgaa ctttagaact
      181 gttttgatac gaggacaagg acatttttgt cactgattca ctatgtttat aaatattttt
      241 tgacatcctc ttgccattcc tctgtcaatt tctttactag aaatccatct ccaattagct
      301 tcgcaaatgt tttagaagac tgataaaatg attcagctaa actaaaatct ctttcttctc
      361 ttaatgcttg cttccacaaa aacataaata caagaggttt cttttgataa tcttgagttt
      421 tatccataat ttcttgtaaa cacgatagat aatcattaaa acaatcagtt actcctacct
      481 tttcaagaca acacattcct gaaataagag tatctctaat tataaagaag ttttctaatt
      541 tagttacctt tttctgttcc aaaagaaaga gcattagctt ttcgatttga tttataaatt
      601 tggcaagatc ttttctaatg agcatttgat aaaaaaacaa acgaatcaac aacatatcat
      661 ttgttgaata aacttctttt tctatgatct gagacatatg ttcctcaagt attaactctg
      721 catattcagg ccttctagtt ctagaagtat ccaaacacgc ctgcaatact tcacatgcta
      781 attgttcttc tttaggaaga ttatcaaagt atgttttata aatctcttcc aaacacgctt
      841 ccttcttatc gtactcctct tctttaccat agattggttc acgtaaaatc tggtatttca
      901 attctaaata agcaggagga agagctgaaa aatccggcat aaggctataa actggaactc
      961 ctagccggcg agcaatatag tctaacttgg gcaaactcgg ttgcgaagct ccactttcaa
     1021 ttcgcgacag ttgacgaact gtcagttctt gctcatctcc acaaaaatct ggacgactaa
     1081 ttcctttttc tatccgaatc tctttaattt tttgaccaat taacatcaga cgcctcctct
     1141 tctacctgct tcgaaaaata ctgtgaaact taaaaaattg taacggctac atctttccta
     1201 ctcataagat ttcatagctt cttccacttc tgccaaagta accccaaaca actctaggcg
     1261 tttgaggagt tgcttgccgt tggaatagcc gattcggaga gtctctccga gatattctct
     1321 acgcttacgg ctgtctgccc ctgctagaaa accaaggcga atcaaatcgc tacgactaat
     1381 gtcaaactga ctctcatgtt caaattgttc tgtcacctga gctagagccg ttttcaggtc
     1441 ttcatagctg gcatgctcaa ttcccagaga acgccccttg gtcttggact tgggaacagc
     1501 ttcatctcgc ttgagaaagg catgctgaac tgttggaatg accgtcatga tcatgcgccg
     1561 aatccgttcc ccattaaaat ctgggtctgt aaagacaatg actccatgac gttggtgtag
     1621 acgctgaatt cgctctatat cctgatcatt gatggcagaa cctcgtgtct cataggtctc
     1681 cacatcgaaa taacgcttga gattgaccgt atcatagcga ccttcaacca cgataacttg
     1741 agaaattttc tctttcatta cttgctgtcc aatccaaaaa ttcgttctgc atttgcagtc
     1801 gttgctaccg ccagctcttc tgtcgtcata ccacgcaagt cagcgataaa gtcgaccaca
     1861 tagcgagtat aggctgtttt attttcacga ccacgcttgg gtacaggtgc taagtaaggc
     1921 gcatctgttt ccaccaacat cttgtccaaa ggtaactctt tagctgcttc ttggaggtca
     1981 gttgccttct tgaaagtcac cactcctgag aaggaaatga tcataccaag atccacaaac
     2041 ttctctgccc actcaagcgt ccctgaaaat gaatgcatga taccaccacg aggaccaacg
     2101 ccctcactct tgataatctc ataggtatct tccagcgcat cacgggtatg gacaacaaaa
     2161 ggcaaatcca agtccttaga tagctgaatc tgacggcgaa aaacctgctc ctgcacctct
     2221 ttgggcgctg tcatccaatg gtagtctaag ccaatttcac ctaaagccac aaccttggga
     2281 tgttttaact tatccaacaa gtaagcctca acttcctctg tataagtacc agcttctgta
     2341 ggatgccaac caatagtcgc atagagctgc tcatactcat ctaccaactc caaggcacgc
     2401 tcaatcgtcg gtttatcaaa accaacaata ttcatctgta tcacacccat ctcagcagcc
     2461 aaggcaattt cttctgcctc acgacctgca aattcttcta cattcaagtg tgtatgtgta
     2521 tcaaaaatca tctcttctaa cctcgttttc tatcttctat tataccaaaa aagaggaggg
     2581 gcacctaatt tttcggtttc ccctcctctc ttcaatagag agctattctg ctatcttttc
     2641 tatccgatat tgcctatctc ctattccaca gttagagaca gaagagattg gctacattga
     2701 tagaatattc ctattcagct aaagtatgtg attatcttgg gttctcactt ttctcaccca
     2761 tcactaggat tgatgacttc tcaaaatccc ccttataatc taactttaag tagagaatag
     2821 aattttctag ctgttctatt ttctacgtct agtctttctt cctagcaaag caaaaccact
     2881 tgttgccacc actagaccaa gactagcaag tgctgcactt gatgctgaac ctgtgttagg
     2941 taattgtttg ccagcaggtg actggtaggt aacattttgc attgttcctg cagttgcttg
     3001 cggtgcactt gttgtcttag gagcagcatt aggagccact agtctcttag gaactgtaac
     3061 catggcaatg gcttgcactc caatcgtttc tgcttccttc acatcttcca tagttttcgc
     3121 attttcaatc tctttgagag ctgcttgttt ttctgcttcc actcttgcta aaagttctgc
     3181 tttttcttta tcagaaagcg gtgcgccttt gatttcatcc tgcttgttct tggctgcttt
     3241 ttcaatggca tttttagctg attctttttc agtagctaat tgcttagaag cttgtgattg
     3301 agctgagttt tgtgaacctg tgtgagctga tccacccgcc tggctaccac cgccttcatt
     3361 cgcattagca tctgagcttc cagtattgcc tcccttatct ccacctggtg tagtttctag
     3421 cttatactct ggtgtagctt cttgagttac agattcacct ttcgcagatt gttgaactgt
     3481 atcttttcct agaatcgtca ctactgaacc atctgggaat gtgattgttg ctgtaccatc
     3541 tccacctact tcaactttat agtaaaataa aataataaca ggacaaatcg atcaggacag
     3601 tcaaatcgat ttctaacaat gttttagaaa cagaggtgta ctattctagt ttcaatctac
     3661 tgtaacatcg cttccgaatc catttgcatc tactgaactt ttttacaaaa ataaaaatca
     3721 gtagactcga gttcctactg atttttgata gaattcgttt ggattatgca gccaaaactt
     3781 tgcgtccttt acgacgacga gctgccaata cgcgacgacc atttttagtt gacatacggt
     3841 tacggaatcc gtgtttgcgc gcacgacgaa gtttacttgg ttgataagta cgtttcacga
     3901 tgaatacctc ctcatagatt ttgtattcgt ttagccggct ataaagtgat cagttactaa
     3961 acatacttta ctattctatc tgattctttc acttttgtca atagctctag gcaaatgttt
     4021 ccagcttttt cgaatgcaag ccctatctac gatactgttt caagaaacga gtcatctttt
     4081 cttggatttg ggctaactca tcaacacgag gaaggtaaac gatacggaag tggtctggct
     4141 cctgccagtt aaagccccga ccatgaacca agagaacctt ttcctgcttc aagaaatcaa
     4201 ggacaaactg ctcatcatca tcgatacggt acatattgcg gtcgattttt gggaagatat
     4261 agagccccgc cttgggttta acggcagaca aacctggaat atcttgaatg gcattataga
     4321 tgaaatttct ttgctcgtag attcgtccac caggaagaag caattcatcg actgattggt
     4381 gcccccccaa ggaagtttgt acgacttgtt gggccaaaac gttagagcaa aggcgcatat
     4441 tggacagcat attgagccct tcgatatagc ccttaacatg agtcttaggg ccagacaaga
     4501 ccatccaacc cacacgaaaa cctgctatgc ggtgggattt tgacagacca ttcatgctga
     4561 cacagaagac atctggtgcc aagctcgcca caggcgtatg cacatgtccg tccattacca
     4621 tgcggtcata gatttcatcc gcaaagataa tcaaatcgtt ttgacgggca atctcaataa
     4681 tctccagcaa gagttcctta ggataaaggg ctccagttgg gttatttgga ttgataagga
     4741 cgattgcctt ggtattggaa gtgatttttg acttgatatc gtcaatatct gggtaccatt
     4801 caacagcttc atcacagata tagtgaacgg catttcctcc agctaggctg acagccgccg
     4861 tccatagagg atagtctggc attggcacta agacctcatc cccattgtcc aaaagcccct
     4921 gcatggacat gacaatcaac tcactgacac catttccaag gtaaatatca tcaatatcta
     4981 cgtttgggaa tttcttcagc tggcaatact gcatgatggc cttacgggct gagaaaatcc
     5041 ctttggagtc agaatagcct tcactatcac gcgcattcat aatcaagtca tgaatgacct
     5101 cgtctggcgc tgtaaagcca aattctgctg gatttcctgt attcagacgt aaaatctttt
     5161 ctccattcgc tcgcatccgc atggcttctt ccaatacagg accacggata tcataagcaa
     5221 catgctctaa cttactagac ttgttatatt ctttcatctt gtttcctcaa ttcatcagga
     5281 tagtaacatt ataccactag aaagagaatg cgacaagttt actgaaatgt gttactagtt
     5341 tcgtatagaa gaaaaacctt gctaaaggcg aggttaagaa cgtagagata gcaggtattt
     5401 tgcaaagaca catcacatac caaaagataa acctagaaaa agcaagccta gaaaggagtc
     5461 ataaagaaga ttaaacatga ggaaaagccc ccatatcatc aagtaatccc aacgcccact
     5521 tcgttttaca actagaaata agattagatg gtagagtaca tgaaagacta aaaatccaat
     5581 aaaacttgga taaagaagcg gtaccagact ctctaattga cagatcatga tcacttctac
     5641 caagactgaa actaggataa ttccataata aaggaaattt gatttttgaa aagagttttt
     5701 catcattctc ccttccttca cttctttcct gctatccttt ctttta
//
LOCUS       DAVMEI010000108         5714 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVMEI010000108 DAVMEI010000000
VERSION     DAVMEI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.108"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(54..2525)
                     /gene="parC"
                     /locus_tag="VYQ24_001541"
     CDS             complement(54..2525)
                     /gene="parC"
                     /locus_tag="VYQ24_001541"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1968665.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQIGIVPILSSYIAHRREVILARSRFDKEKSEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(2945..4888)
                     /gene="parE"
                     /locus_tag="VYQ24_001542"
     CDS             complement(2945..4888)
                     /gene="parE"
                     /locus_tag="VYQ24_001542"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1968666.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            5024..5665
                     /gene="plsY"
                     /locus_tag="VYQ24_001543"
     CDS             5024..5665
                     /gene="plsY"
                     /locus_tag="VYQ24_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1968667.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         1523 a         1334 c         1113 g         1744 t
ORIGIN      
        1 tcagaaaatt tctccaataa aaaccagctc ttagaactga ttcttcattt cacttattta
       61 tcttcagtaa ctacttcctg aagataagcg tcaaaaactt cttcatctga aatcgtgtca
      121 gaaatgaagc ttccattgct agtgcgttct gacaagttca agtcttgcaa tcggctttca
      181 tagattgttc ctttattgga ttggacaagc agagtttggt cgttcacatc cacttccgta
      241 ctgaagaaat cgccaacaaa tccttgctct gcaactgctc ctgccaagaa gacacgatgc
      301 ggtttgtttt tcaactcacg caagacttgt aatcctcgtt tggcacggct ggttgctaga
      361 atttcctcaa tggaaacacg tttcaagctt ccacgctggg tcaagaggta gaaggacgaa
      421 gtattacaga taaagccaga ttggaggaca tcatcttctt tcaaattcat agccttgaca
      481 cctgctgcct tagcaccgac aaccggaacc tcttcgatat tgaaacgcag ggcataacca
      541 ttttgactaa ccaagacaac atcatctagt ttaatcggag ccactgctac aatctgatct
      601 gtatcgtctt tgagcttagc atacttgaca gacttagatc tataggtccg ccatggagtg
      661 aattcttttc gctctacccg tttgatttga ccaaggcgag tcactgcaaa gtaggttgtc
      721 gcatcgtcaa actgatccag tacttccaca taaaggattt cttcattcgt ttcaaagttt
      781 gtgatggttt ggctcagatg ctctccgatg tccttccaac gaatatctgc caactcatgg
      841 attggtctgt agatgacatt tccaagactt gtgaacatca agaggtgctg ggttgtcttg
      901 gcagattgaa caaaaatcaa acggtcatca tcacgcttgc caatttcttc caaggtggaa
      961 gccgcaaagg aacgtggact ggtacgcttg atgtaacctg ccttggtcac gctgacgtag
     1021 gtatcttcct cagcgataag actagctgta tcaatctcaa ttgctttcgc agtgtcttct
     1081 aaagaactca aacgaggagt tgcaaatttc ttcttgacct cacgaagttc tttcttcatg
     1141 agattgtaca tagtcctttc atcaccgata atagccgcca gcatagcaat cttctcacga
     1201 agctctgctt cttcttcctg caagacaacc acatcggtat tggtcaaacg gtacagttgc
     1261 aaagttacga tagcctcagc ctgttcttcc gtaaaatcat agctaacttt gaggttttcc
     1321 ttggcgtccg ccttattctc agaagcacgg ataagagcaa tgacttcatc caaaatcgaa
     1381 atcacacgaa tcaaaccttc gacgatatgg agacgtttct cagacttttc tttgtcaaag
     1441 cgtgaacgcg ccaaaatcac ttctcgacgg tgagcgatat agctagacag gattggaaca
     1501 atcccaatct gacgaggtgt gaaattgtca atcgccacca tattaaagtt gtagttgatt
     1561 tgtaggtcgg tgtacttaaa taagtagttg agaacaagct cagtattagc gtctttctta
     1621 agttcgatag cgatacgaag accatcacgg tcagactcat cacgaacctc agcaatccca
     1681 gctaccttgt tattaacacg aacatcatcg attttcttga ctagattggc cttattgatt
     1741 tcataaggaa tctcagtaat aacgatttgt tccttaccac cttttagctt ttcaatttca
     1801 gtcttggaac gaacaaccac gcgccctttc ccagtctcat aagctttctt gatttcatca
     1861 cgaccctgaa taatagcccc tgtagggaag tctggtccag gcaagaattc catgagttta
     1921 tcaatctttg cagttgggtg gtcaatcatg taaactgcag catctatgac ctcagctaaa
     1981 ttatggggag gaatgtctgt ggcataacca gccgaaatcc cagtcgaacc attgaccaag
     2041 aggtttggaa aggctgctgg caagaccgtt ggttctttct ccgtatcgtc aaagttccat
     2101 gcaaaaggaa ctgtcttttt ctcgatatcc tgaagaaggt agcctgcaat ttcagacaaa
     2161 cgtgcctcag tataacgcat agccgcagga ggatctccgt ccatagaacc gttattaccg
     2221 tgcatttcaa ctagaatctc acgatttttc cagttctgtg acatacgaac catggcatca
     2281 tagatagaag aatccccgtg tgggtggaaa ttccccatga tgttcccgac tgacttggcc
     2341 gacttacggt agctcttgtc aaaagtattg ctatccttat tcatagaata aagaatacgg
     2401 cgttgaaccg gcttcaaccc atcacgaata tctggcaaag cccggtcttg aataatgtac
     2461 ttggagtagc gaccaaagcg ctctcccatg atgtcctcca gggacatgtt ttgaatgtta
     2521 gacataagat acaaagccca taaaatacca agtgaaaata gaaaattctt gaagtaagca
     2581 aactcacaag agaatttatc tttttcacac agtatctagg gcgtgttcaa ctcctttcaa
     2641 agaatgtaga gtaggttttt atgcagtaaa agatatttta cgggaattcc tcccgtgttc
     2701 agttacgata agtaaccaaa ctatcctgtt tgtatttttc aatatgaaaa tctggttttc
     2761 caaaattagt cttagtttgt gtcttaaccg ctcccttaag cgcctcttta agataagcac
     2821 tcatagcaga ttcttcatta ataatcctgc aattttttca aaccaagatt ttcaaactgc
     2881 tttttcacat agtcattcac atccgactct aatttccagt ttactaacat attattttct
     2941 ttcattaaaa cactgtcgct tcttctagcg taaacttgac attatcttca atccatttac
     3001 ggcgtggttc taccttatct cccatgagaa cattgacgcg gcgttcggcg cgcgctaaat
     3061 cttcaattgt gacacggatg agggtacgtg tttctgggtt catggttgtt tcccagagct
     3121 ggtccgcatt catctcacca agtcctttgt atcgttggag ggtagcgcct ttaccgaact
     3181 gtttacggag ttcttctagt tctccatccg tccaagcgta ggccacttct tctttcttgc
     3241 ctttaccttt ggacatcttg taaagaggtg ggagggcaat atagacatga cctgcctcga
     3301 ctagcggacg catgtaacgg tagaaaaatg tcaagagcaa ggtctggata tgggcaccgt
     3361 cggtatccgc atcggtcatg ataatgatct tatcatagtt ggcatcttca atagagaagt
     3421 ctgctccaac acccgcacca atggtataaa tcatggtatt gatctcttca tttttgagga
     3481 tatccgccat cttggccttg gctgtattga caaccttacc acgaagaggt agaatagcct
     3541 ggaacttgcg gtcacgacct tgtttggcag aaccaccggc agagtccccc tcaactagat
     3601 agagttcatt cttagcagga ttcttagatt gggctggggt caatttccca gacaacaagc
     3661 ccttatcttt cttgtttttc ttcccatttc ggctctcatc acgcgcctta cgtgctgctt
     3721 cacgagcatc acgggccttg atagccttgc ggatgaggtt agaagctaat tccccatttt
     3781 ccataaggaa aaaggtcaac ttatcagcca ctattccatc cacaactggg cgagctaggg
     3841 ggcttcctag tttatccttg gtctgtcctt caaactgcaa gtgttcttca ggaactaaga
     3901 tagaaagaac ggccgctagt ccctcacgat agtctgaacc ttcaaggttt ttatcttttt
     3961 ccttgagaag acctgtttta cgtgcatagt cattcatgac cttggtaatg gcagacttga
     4021 gtcctgtctc gtgcgttcca ccgtccttgg tgcgaacgtt attgacaaag gatagaatgt
     4081 tatctgagaa tccgtcattg tactggaggg ctacttccac ttgaaaacca ttgtcttccc
     4141 cttcaaagta aagaactggc gtcaagattt ccttatcttc gttgagataa gaaacaaaat
     4201 cttgtactcc attctcatag tggaactcaa tcgcttcatc tgttcgcttg tccgttaaag
     4261 acaaggtcac atttttcaag agaaaggctg attcattaag gcgctctgaa atggtattgt
     4321 acttgaaatc tgtcgtagaa aagatagtcg cgtcaggcat aaaagtaact ttggtgcctg
     4381 ttttagactt gggtgctgta ccgattttct tcaaagtcgt gacaggtttt ccaccatttt
     4441 caaaacgttg cttgtaaact gcgccatcac gggtaatttc aacttctaac cagctagaaa
     4501 gggcgttaac aacggaagaa cccactccgt gaagtccacc tgatgtctta tagccacctt
     4561 gaccgaattt ccctccggca tgaagaatgg taaagataac ctcaacagtt ggaattccca
     4621 tagcgtgcat acctgtcggc atcccacgtc catggtcttg aaccgttaga ctaccgtctt
     4681 tattgatagt tacatcaata cgatcaccaa acccagacaa ggcttcatcg actgcattat
     4741 caacgatttc ccaaactagg tgatgaagac cagcgccatc ggtcgatcca atatacatcc
     4801 ctggacgttt tcggaccgca tccaaccctt ctagcacctg aatagcatca tcattataat
     4861 tgttaatatt gatttccttt tttgacacaa ggaacctcct attcgttcat ctttactatt
     4921 ctacaggttt tccaaggatt ttgcaaaatt tttctttctc cgatgtgaca attttagcag
     4981 agattctctg cttttctttc ccaattcatg atataatagg agtatgatta caatagtttt
     5041 attaatccta gcctatctgc tgggttcgat tccatctggt ctctggattg gacaagtatt
     5101 ctttcaaatc aatctacgcg agcatggttc tggtaacact ggaacgacca ataccttccg
     5161 cattttaggt aagaaagctg gtatggcaac ctttgtgatt gactttttca aaggaaccct
     5221 agcaacgctg cttccgatta tttttcatct acaaggcgtt tctcctctca tctttggact
     5281 tttggctgtt atcggccata ccttccctat ctttgcagga tttaaaggtg gtaaggctgt
     5341 cgcaaccagt gctggagtaa ttttcggatt tgcgcctatc ttctgtctct accttgcgat
     5401 tatcttcttt ggagctctct atcttggcag tatgatttca ctgtctagtg tcacagcatc
     5461 gatcgcggct gttatcgggg ttctgctctt tccacttttt ggttttatcc tgagtaacta
     5521 tgactctctc ttcatcgcta ttatcttagc acttgctagt ttgattatca ttcgtcataa
     5581 ggacaatata gctcgtatca aaaataaaac tgaaaatttg gtcccttggg gattgaacct
     5641 aacccatcaa gatcctaaaa aataaaacgc cagttctgta ctgcccccaa acagttagac
     5701 aattaattta tccg
//
LOCUS       DAVMEI010000109         5707 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVMEI010000109 DAVMEI010000000
VERSION     DAVMEI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.109"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            117..512
                     /locus_tag="VYQ24_001544"
     CDS             117..512
                     /locus_tag="VYQ24_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1968668.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            523..894
                     /locus_tag="VYQ24_001545"
     CDS             523..894
                     /locus_tag="VYQ24_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1968669.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMWEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            904..3147
                     /locus_tag="VYQ24_001546"
     CDS             904..3147
                     /locus_tag="VYQ24_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1968670.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            3354..5129
                     /gene="spxB"
                     /locus_tag="VYQ24_001547"
     CDS             3354..5129
                     /gene="spxB"
                     /locus_tag="VYQ24_001547"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1968671.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            5240..5587
                     /locus_tag="VYQ24_001548"
     CDS             5240..5587
                     /locus_tag="VYQ24_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1968672.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
BASE COUNT         1696 a         1061 c         1312 g         1638 t
ORIGIN      
        1 caaatgatta aaattctatt tttcgaagaa aatattattt ctataattga caaatgtaga
       61 ttttgggagt atactgttga gtgtaattga caaatgtaga ttttggaggt atggtaatgc
      121 agatttcaga tgcagaatgg caggtcatga agattatttg gatgcagggg gagcagacca
      181 gtacagattt gattagggtt ttggcagagc ggtttgactg gtccaagtcc acggttcaga
      241 cacttttggc tcgtctagtt gagaaagagt gtttgactcg gaaaaaagaa ggcaagtcct
      301 ttgtttattc agcgctttta actctagacc aaagtcggga tttacttgtc caagatatca
      361 aagacaaggt ttgttcccgt aggattagga atttgttggc tgacttgatt gtagaatgtg
      421 aatttactca gactgacttg gaagacttgg aagctgtgat ttcagagaag aaatcaagtg
      481 ctgtaacaga agtaagatgt aattgtatgt aaaggagacg tcatgttaaa tagtattgta
      541 accattattt gtattgccct tatcgcgttt atcttgtttt ggtttttcaa aaagcctgaa
      601 aaatctggac aaaaagccca gcaaaaaaac ggataccaag agattcgagt ggaagtcatg
      661 ggaggctata ctcctgagtt gattgtcctc aagaaatcag tgccagcccg cattgtcttt
      721 gaccgcaagg atccttcacc atgtctggat caaattgttt ttccagattt tggtgtacat
      781 gcgaacctgc caatgtggga agagtatgta gtggaaatca cgcctgaaca ggctggagag
      841 tttagctttg cttgtggtat gaacatgatg cacggcaaga tgattgtaga gtaggtggag
      901 actatgacag aaattgtgaa agcaagctta gaaaatggca ttcaaaaaat ccgtatccaa
      961 gctgaaaaag gctatcatcc agcccatatc cagcttcaaa agggaattcc agctgagatt
     1021 acctttcatc gtgctactcc ttcaaactgt tataaggaaa ttctgtttga agaagaaggt
     1081 atcttggaac caatcggcgt agatgaggag aaagtcattc gttttacacc tcaagaatta
     1141 gggcgacatg aattttcttg tggcatgaag atgcaaaagg gaagctatac agtcgttgag
     1201 aagactcgaa aatctctatc tctcctgcaa cgtttttgga ttactagtat ctgtactgtg
     1261 cctcttgtga ttctcatgat tgggatgttg gcaggtagca ttagtcatca agtcatgcat
     1321 tggggaacct ttctagcaac aacgcctatt atgttagttg caggtaagcc atatatccag
     1381 agtgcttggg ccagttttaa aaagcacaat gccaacatgg ataccttggt tgcgctggga
     1441 actctagtgg cttatttcta tagcctagtt gctctctttg ctggtctccc tgtttacttc
     1501 gaaagtgctg gatttatcct ctttttcgtt cttttgggag cagtttttga ggaaaaaatg
     1561 agaaaaaata cgtcccaagc tgtggagaaa ttactggact tgcaagctaa aaccgcagaa
     1621 gtcttgagtg atgatagtta tgtccaagtt cctttggaac aagtcaaggt aggcgacctg
     1681 attcgagtgc gtcccggtga aaagattgct gttgatggtg tcgtagtaga aggtgtctct
     1741 agtattgacg aatccatggt gacaggtgag agtctgcctg tggacaagac agttggagat
     1801 actgtcattg gctcaaccat caatcatagt ggaacgcttg tctttagagc agaaaaagtt
     1861 ggctcagaga ctgttttggc tcagattgtg gattttgtga agaaagctca gacaagtcgt
     1921 gcgccgattc aggacttgac ggataagatt tcagggattt ttgtcccagt agttgtcatt
     1981 ttaggaatca tgaccttttg ggtttggttc gtcttgctca gggatagtgt ggttgtgctt
     2041 ggagctagct ttgtgtcctc tcttctctac ggagtggcgg ttttgattat cgcctgtcct
     2101 tgtgccttgg gacttgcaac accgacagcc cttatggtgg ggacaggacg tagtgccaag
     2161 atgggggttc tcctcaaaaa tggaactgtc ttacaggaaa tccagaaagt tcaaaccctt
     2221 gtctttgata agaccgggac tttgacggaa gggaaacctg tggtaacaga tgtcatcggc
     2281 gacgaagtag aagtgtttgg attggcagcc tccttggaag atgcttctca acacccactg
     2341 gctgaggcta tcgttaagcg agcgagtgaa gctggacttg agtttcaaac tgttgaaaat
     2401 tttcaggcct tgcacgggaa aggtgtttca gggcgaatca atggaaaaca agttttactt
     2461 ggaaatgcta aaatgctgga tggcatggat atttctaata cttatcaaga taaactagaa
     2521 gaactagaaa aagaagctaa gacagttgtg ttcttagctg ttgacaatga aatcaaaggc
     2581 ttgcttgctt tgcaagatat tcctaagaaa aatgctaagc tagccatcag tcagctaaaa
     2641 aaacgtggtc tcgggacagt catgctgaca ggagacaatg ctggtgtggc gcgtgctatt
     2701 gcagatcaaa tcggaattga agaggtcatt gcaggcgtct tgccagaaga aaaagcccat
     2761 gaaatccata aactgcaaca gtcaggtaaa gtagcctttg ttggtgatgg gatcaatgac
     2821 gctcctgccc ttagtgtagc agatgtgggg attgctatgg gagctggaac agatatcgcc
     2881 atcgagtcag cagatttggt gttgacaacc aataatcttt taggagtggt gcgtgccttc
     2941 gacatgagta agaaaacctt tcatcgtatt ctgctcaatc ttttctgggc ttttatctac
     3001 aatgttgtcg gaattccgat tgcagcagga gtcttttcag gtgttggact ggctctcaac
     3061 ccagaattgg caggtctagc catggccttt agttctgtat ctgttctgac cagttccctt
     3121 ctgttaaatt ttagtaaaat agactaaaag taatgactag atttttttgt tataaaacag
     3181 aaatgacgac actttcaaaa ctgtattaga atagagaata gagagttttg agcagatttt
     3241 tagaaaagtc agcataaata tgatacagtg gaatagtaaa aatttggaga acgtttccaa
     3301 ttctatgtaa tcgtattctc caagtctaaa aaaattgaag gagagttatc attatgactc
     3361 aagggaaaat tactgcatct gcagcaatgc ttaacgtatt gaaaacatgg ggcgtagata
     3421 caatctacgg tatcccatca ggaacactca gctcattgat ggacgctttg gctgaagaca
     3481 aagatatccg cttcttacaa gttcgccacg aagagacagg tgctcttgca gcggttatgc
     3541 aagctaaatt cggcggctca atcggggttg cagttggttc aggtggtcca ggtgcgactc
     3601 acttgattaa cggtgtttac gatgcagcta tggataacac tccattccta gcaatccttg
     3661 gatcacgtcc agttaacgaa ttgaacatgg atgctttcca agagcttaac caaaacccaa
     3721 tgtacaacgg tatcgctgtt tacaacaaac gtgtagctta cgctgagcaa ttgccaaaag
     3781 taattgacga agcctgccgt gctgcagttt ctaaaaaagg tccagctgtt gttgaaattc
     3841 cagtaaactt cggtttccaa gaaatcgacg aaaactcata ctacggttca ggttcatacg
     3901 aacgctcatt catcgctcct gctttgaacg aagttgaaat cgacaaagct gttgaaatct
     3961 tgaacaatgc tgaacgccca gttatctatg ctggatttgg tggtgttaaa gctggtgaag
     4021 tgattactga attgtcacgt aaaatcaaag caccaatcat cacaactggt aaaaactttg
     4081 aagctttcga atggaactat gaaggtttga caggttctgc ttatcgtgtt ggttggaaac
     4141 cagccaacga agtggtcttt gaagcagaca cagttctttt ccttggttca aacttcccat
     4201 ttgctgaagt ttacgaagca ttcaagaaca ctgaaaaatt catccaagtt gatatcgacc
     4261 cttacaaact tggtaaacgt catgcccttg acgcttcaat ccttggtgat gctggtcaag
     4321 cagctaaagc tatccttgac aaagtaaacc cagttgaatc aactccatgg tggcgtgcaa
     4381 acgttaagaa caaccaaaac tggcgtgatt acatgaacaa actcgaaggt aaaactgagg
     4441 gtgaattgca attgtatcaa gtttacaatg caatcaacaa acatgctgat caagacgcta
     4501 tctactcaat cgacgtaggt aacactactc aaacatctac tcgtcacctt cacatgacac
     4561 ctaagaacat gtggcgtaca tctccactct ttgcgacaat gggtattgcc cttcctggtg
     4621 gtatcgctgc taagaaagac aatccagatc gccaagtatg gaacatcatg ggtgacggag
     4681 cattcaacat gtgctaccca gacgttatca caaacgttca atacgacctt ccagttatca
     4741 accttgtctt ctcaaatgct gagtacggct tcatcaagaa caaatacgaa gatacaaaca
     4801 aacacttgtt tggtgtagac ttcacaaacg ctgactacgc taaaattgcg gaagctcaag
     4861 gagctgttgg attcacagtt gaccgtatcg aagacatcga tgcagttgtt gcagaagctg
     4921 ttaaattgaa caaagaaggt aaaactgttg tcatcgatgc tcgcatcact caacaccgtc
     4981 cacttccagt agaagtactt gaattggatc caaaacttca ctcagaagaa gctatcaaag
     5041 ccttcaagga aaaatacgaa gcagaagaac tcgtaccatt ccgtctcttc ttggaagaag
     5101 aaggattgca atcacgcgca attaaataat tcctctcgcc gaaaatcaaa tatgaaactt
     5161 gtagacttta ttctttgatt ttcatagagc agaactttaa aagatcggag caatccggtc
     5221 tttttatgga ggagagtaaa tgaatttaaa tcaattagat attatcgttt caaatgttcc
     5281 ccaagtctgt gctgacttgg agcatatttt ggataaaaag gcagattatg ctgatgatgg
     5341 ttttgctcag tttacgattg gtagtcactg ccttatgttg tcacaaaatc atttggttcc
     5401 tttggaaaac tttcagtcag gaatcattat tcatatcgag gttgaggatg tagaccagaa
     5461 ctacaaacgg ttgaacgagc ttggtataaa ggttttacac ggaccaactg taaccgattg
     5521 gggaacagag tccttattag ttcaaggacc tgctggtcta gtgcttgatt tttatcgtat
     5581 gaaataggtc gcgtagtcat ttgacgttaa ccttagctat ttttaaaata gaaattgaga
     5641 atatgaacta ttgaaagatc ggagcaatcc ggtcttttta tataaagaaa ttgcatataa
     5701 tactcat
//
LOCUS       DAVMEI010000110         5507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVMEI010000110 DAVMEI010000000
VERSION     DAVMEI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.110"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(133..1101)
                     /locus_tag="VYQ24_001549"
     CDS             complement(133..1101)
                     /locus_tag="VYQ24_001549"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1968673.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVAVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1246..2029)
                     /locus_tag="VYQ24_001550"
                     /pseudo
     CDS             complement(1246..2029)
                     /locus_tag="VYQ24_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(2054..2551)
                     /locus_tag="VYQ24_001551"
     CDS             complement(2054..2551)
                     /locus_tag="VYQ24_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1968674.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2624..3985)
                     /gene="radA"
                     /locus_tag="VYQ24_001552"
     CDS             complement(2624..3985)
                     /gene="radA"
                     /locus_tag="VYQ24_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1968675.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPQNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(3999..4514)
                     /locus_tag="VYQ24_001553"
     CDS             complement(3999..4514)
                     /locus_tag="VYQ24_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1968676.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4516..4959)
                     /locus_tag="VYQ24_001554"
     CDS             complement(4516..4959)
                     /locus_tag="VYQ24_001554"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1968677.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            5264..5413
                     /locus_tag="VYQ24_001555"
     CDS             5264..5413
                     /locus_tag="VYQ24_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968678.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT         1721 a         1325 c          971 g         1490 t
ORIGIN      
        1 atgacgtaca gtcatatttc ttctcttttt attatatcac agttttaaat ctagctttac
       61 tagattcacc gctactatct atttattcgg aaaaaagacg aaaaaacctg agaatcatct
      121 caggcttggt cattaaattt ttttctcaat atcgaaaagt ggagaaagtg gtcgtttttc
      181 atgaatacgt acgatagcat cccctaggag atgagcgatt gaaatctgct caatcttatc
      241 aatcaaacgc tcttctggaa gatagatggt atccaaaaca accaatttct taatagctga
      301 tttttggata ttgtccgtag caggaccaga aagaactggg tgcgtacagc ttgcatagac
      361 tgcaacagca ccagcttccg caagagcatc tgccgcatga caaatcgttc cagcggtatc
      421 aatcatatca tcaatcaaga tacaagtctt gccttcaacc ttaccgatga tgttcataac
      481 ttcactagta ttcatcttat caacgctacg acgtttatca ataatagcga tagatgtttt
      541 caaaaattct gccaacttac gagcacgagt cacccctcca tggtccgggc tgacaaccac
      601 atagtcagaa ccaaccatac cacgccgctc aaaataatct gcaattagag gagcacccat
      661 caaatgatcc acaggaatat caaagaatcc ttgaatttgc gcagcatgca agtcgatggt
      721 caataaacga tccactccag ctacttcaag catatttgcg acaagttttg aagtgattgg
      781 ctcacgcgct ctcgcctttc tatcctgacg tgcataccca tagtaaggca tgacaacatt
      841 gacagactct gcactcgcac gcttcaaagc atctaccata atcaaaattt caagcagatt
      901 gtcatttaca ggcgaactag ttgattgtaa gataaagacg tgtttcccac ggattgattc
      961 ttcaatgttg acctgaatct ctccatctga aaattggcga acacttgatt tccccaactc
     1021 tatcccaatc tcctgcgcca cacgttctgc caattcttta ttagaagaaa gggcaaacag
     1081 ctttaaatca gaaaaagaca tgatttcctc cggtatatat gtatcgcttg tgcttttcac
     1141 aagattttcc atctaccatt gtagcgcttt ttgcactatt tttcaatcaa aaataaaaga
     1201 agggcaccat atttgtaccc ttgcatcatt cttttgaaaa atattctagg tcatcaactc
     1261 attgtgtttc tcaacaaagc aataagcatg ataataacca tagagagcaa tagccgtaac
     1321 cactggaatc gctaaaggca actctgtttc caactccaca aaaggagagt taaacaataa
     1381 gaccctgttt cttgccaacc ttctgtcctt tataggctct gtaaagcaag taaacaccta
     1441 ctacagctag acctgaaaaa gtccagtgag aggcaattcc tgagatgata cgctctaaaa
     1501 ttcgcgaaat agtaaagtca aagccctctg gcaaatccgt acgaatataa ccaatatcct
     1561 caatcatttg gaatcccaaa ccggaggcaa ttccaagtaa aaacaaaaat tttaattttc
     1621 gcacaggaat caaagccaaa acaaaaacaa gtggcaataa tttcaagggt tcttctacca
     1681 aaggagccac aatagcactt tcaaaggcat ttaaaaatgg actatctggg aaaagaaccc
     1741 ccagtaaatc atgtatataa gtattagcaa aactagacaa ccagcctgaa aggaacatcc
     1801 ctcccaataa agacagaatc aaaaccttct ttggcaattc ccattttttc ccaatacgga
     1861 agagaaaata aagagccgga atcatgtaaa agagagctag aaagatagaa actcccatta
     1921 gtccatattc cgcacctgac ctcgaaccgt ccgtatagta gatggtttca tactgtaaac
     1981 caatacatag caataaaata aaaataaata aaatactgct tttcttcata cactttcttt
     2041 ctaaatgaag tatttataat tctacgactg tcatacttcc tgtatcaaca ttgtaaatag
     2101 caccagagat aatgacatcg tctggtatta ggggagactc gataagcagt tgcatatcct
     2161 cgcgtacact ctcttctata tcttggaagg gcaagaagtc ctggtctgac acatctacac
     2221 ctaattcctc ttttaaatac tcctgaaaag gttcattttc aaaggtctga gcaccacagt
     2281 ctgtatggtg caataccaca atctctcttg tccccatttg ttgctgggaa ataactagcg
     2341 aacgaatcat gtcttcagtc actcgaccac ctgcattccg caagatatga gcatccccaa
     2401 gtgccaagcc cagagcttgc gcaacgtgca aacgtgagtc catacaggtc acaatggcta
     2461 ctctggtttt aggtttaagt ggcagattta actgcccatg tagggcaaca taagcctgat
     2521 tggcttgcat aaactgttca aaatacgaca cgattccctc cttgaaaatt tgatagtcaa
     2581 atatttctcc tatcttatca tttttaagag aatttgtcac ggattatgca aagacctttt
     2641 tcaagacttc ctgaatcgtt gtcacgccaa tgacctgaat ttccttaggc agagtgattc
     2701 ctgtcaagga attctgaggt acataaatct tagtaaagcc cagtttagca gcttcgttga
     2761 tgcgttgctc aatacgattc acgcgccgaa tctctcctgt caagcccagt tctccgacaa
     2821 aacattcctg aggattagtt ggcttgtctt tgtagctcga agcaatagca actgcaacag
     2881 ccaagtcaat ggcaggttca tccaatttaa caccaccagc agatttgaga taggcatcct
     2941 gattttgcaa gagaagccct gcccgttttt ccaaaacagc cataatcaag ctagcacggt
     3001 taaaatcaag tcctgtcgta gtacgcttgg catttccaaa catggtcggt gttaccaaag
     3061 cctgaacctc cgccaaaatc ggacgcgtcc cttccatggt tacaacgatg gaggaaccag
     3121 tcgccccatc caaacgctct tctaggaaaa cttgactcgg attgagtacc tcaaccaagc
     3181 cgcccgactg catctcaaaa atcccaatct cattagtgga accaaaacga tttttgaccg
     3241 ctctcaaaat acgaaaggtg tggtgacgct ccccttcaaa gtaaagcacc gtatccacca
     3301 tatgctccaa catacgaggc ccagccaagg ttccttcttt ggtcacatga cctacgataa
     3361 agatggcaat gttattggtc ttggccaact gcatgagttc agcggtcact tcacgtacct
     3421 gagaaacaga cccctgcacc cctgaaatct caggagacat gatggtctgg atggaatcaa
     3481 taatgagaaa gtctggctgg atacgctcca cttctgcacg aacactctgc atattggtct
     3541 ctgcatagag ataaaactca ctatcaatat cacctaagcg ctctgcacgt agtttaatct
     3601 gctgggcaga ctcctcccca ctgacataga gaactgtccc cacttgggac aactgggttg
     3661 agacttgtag gagaagagtt gatttcccaa tcccaggatc cccaccgatg aggacgagac
     3721 ttcctggtac cactccgcct ccaagcacac ggttgaattc ctccatctcc gtcttggttc
     3781 gattgacatt gatggaagtc acctcagcta gtttcatggg cttggttttc tcacctgtca
     3841 aggacacacg cgcattcttg acctcggcaa cctcaacctc ttccacaaaa gaagaccaag
     3901 acccacagtt ggggcaacgt cccagatatt taggggaatt atacccacaa ttttgacata
     3961 caaatgtcgc ttttttcttt gcgatgacaa acctctttct atatctctaa ctcacactca
     4021 atcacttggc aaaaatcaat cttctcattt ggcacaaact ggcgcatgag cattcgatga
     4081 gcaacaacta ccacagtctg atgttctcga tacttagaca tacattctag aaaccgagac
     4141 ttcatttccg tagctgtctc atattgaata ggactattag gaagcaactc ccccttgttt
     4201 tctaaaaaca gtcttctagc tgtttcaaag ttttctattc ctgttttata gacctgccat
     4261 tcatgtaata aaggctctac tcttaaagga agacccgtag cacagaccac atacgaagcc
     4321 gtttctaaag ctcttgtgac tgcagaagat acgattattt cagctgacga gagtaaagga
     4381 tttttgctca atttctggac ttgctgccgt cccatctcag acaagggtgc caaatctatc
     4441 ccaaatccta tataagaacg ctcctctaac tcacggtaat ctggctcccc atgacgtaca
     4501 aagataatct tcattctagt gccctgtcga tccaaatcca ccagttcgaa cgccatcagc
     4561 tgcatctcca tctgcaatta agaaagtagc aaaaacagcc tggacaatac gttccccaac
     4621 ttcaagaaca acctcttgat ctgtgatatt cttcatctgc gcaaaaatat gcccttcatt
     4681 tccaggattt ccataataat ccccatcaat gaccccaact gagttaatta aaaccaagcc
     4741 cttcttacga ggatttgaag aacgatcata gaggtagaga acctcagtcg gctgcatata
     4801 agccttaacc cctgtcggaa ccaagacaat ctctcctggc gcaacaactg tacgcacagc
     4861 aacctttaag tcgtaaccag ccgcatgcgc tgtctcacgc ttgggcaata aattttcatc
     4921 tgtaaaactc gaaaccaatt caaaaccacg aattttcata attttctctt ttctattatc
     4981 atttattcta gattattcta tcttatttat tcggaaaaag cacgaaaaaa agagcacaca
     5041 acaattatag gcgatacgat aatttacact gtttcacaat acgttgaaat ttagagcttt
     5101 aaagcgaggg caaagttgat tttttacatc gttttacaga ggtttacgac acttttgccc
     5161 cttttttgcc cctttgtttt tcaaaaaaac ttcatcaaat cgcttgactt tcacggttta
     5221 ccgtgatata atataatcaa gataaggaaa ggaggtgagg aagttgaaca aagaagattg
     5281 gcttaggtta ctggaaaagg cgatagataa tatccctgaa acggtaactg ctatagcaag
     5341 tctggtgact gcaataacgg tcgcaaggca aaacaaaaag cgtaaaccga aatcccgtca
     5401 aagaaaaagg tgaacgctaa gaggttgggg cgaaagcccc tcacacctct attttatcaa
     5461 atgaaaagag gaaaagcaat ggttagtgca atagctattt ttataat
//
LOCUS       DAVMEI010000111         5472 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVMEI010000111 DAVMEI010000000
VERSION     DAVMEI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.111"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..915)
                     /locus_tag="VYQ24_001556"
     CDS             complement(<1..915)
                     /locus_tag="VYQ24_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1968679.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     SVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSSLCELFSDDGMTKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLE"
     gene            complement(912..1769)
                     /locus_tag="VYQ24_001557"
     CDS             complement(912..1769)
                     /locus_tag="VYQ24_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1968680.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRLTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(1773..3119)
                     /locus_tag="VYQ24_001558"
     CDS             complement(1773..3119)
                     /locus_tag="VYQ24_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1968681.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(3132..3416)
                     /locus_tag="VYQ24_001559"
     CDS             complement(3132..3416)
                     /locus_tag="VYQ24_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1968682.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(3420..5450)
                     /locus_tag="VYQ24_001560"
     CDS             complement(3420..5450)
                     /locus_tag="VYQ24_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1968683.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         1840 a         1164 c          737 g         1731 t
ORIGIN      
        1 ttctagaacc ttctctttta tattcgattc actcaaacca tactcattaa gaagataatc
       61 cattttccct acttgaccaa atctttcttg aacacccatc cgatgaattt ttgtcattcc
      121 atcatcagag aataattcac ataaagaact gccaattcca cctatctgat tgtggttttc
      181 tacagtaaat atagtttttc cacttaacat tgtttttatc tgttctggta tcggtttgat
      241 tctaaataaa tctatcacac ttactgaata acctaattta gacagttcat ctgcaactcg
      301 aatacttgga gcaaccatta taccagaagc aacgattaca agatcttcac catgccttaa
      361 ctcaatgtag cctttagaaa aatcttctcc accttgatac acaggaactg gagcttttct
      421 aattgttcga atatatttta gtccttttaa gtccaatgtc tggttcaata tttcacgaaa
      481 ttggatatca tcagttgctt caaaaatgat cgatttagga attaaacgta acaatccaat
      541 ttcttcaaat ggcatatgtg tcccaccatt catttctgca gttactcctg catctgatcc
      601 aatcacagtg gcatccaatt gtgcgtatcc aagagaaata aataattgat caaatactct
      661 tcgtgaagca aaaggaccaa atgtatgaag ataaggtcta aacccctgaa tagacaagcc
      721 tgctgcaagg ccgaccattt ctgcttccat aatcccaaca ttcacataac ggtctccaaa
      781 gtccttttca agattatgag tagccatcga acttgacaaa tcggcttcta agactactat
      841 atcagaataa ctttgattag cctctaaaag gaagtctcta tatacatgcc gtaattcttt
      901 cgtacttctc atcattctgt ttcctccaat tcctgactta atctttctac aactgaagtt
      961 aacatttgtt tctcctctac agtaaggcga agatgatgat tggatttcat ttcttccagc
     1021 tcttgaaccc cttgaccttt aatagtatct aatacaatgc acttaggtga tgaattattt
     1081 gactgtttta attggacaat cccttcataa atttctctaa tatctgaacc cttgacccta
     1141 atggattcaa atccaaatgc tgaaaatttt tctacgaaat cacctggatt acaaatatcc
     1201 tttgtaaaac catctaattg ttttttgtta tcatcaacaa atacaattaa gttggataac
     1261 tgttgatgag aagcaaactg tatagcctcc caacattgtc cctcatttaa ctcaccatct
     1321 ccaacaatag cataagtata aaagggactc tttcttattc tctgaccata tgcaagtcca
     1381 gttgcaacac taattccttg tcctaaagag cccgttgtca tatctatgcc tggcgttaga
     1441 tttctatcag gatgagacgg taatttggtt ccatttgtat ttaaagaata taggaattct
     1501 ttgtcaaaga aaccattcaa atagagtgta ctgtatagag ctggtcctcc atgacctttt
     1561 gataatatga aataatctct atctcgtgct gcaaatattt ctggagtcat tggcattatt
     1621 tcaccataaa gcactgctaa aacttctacg atagacagac ttcctccgta atgtccgaat
     1681 ccaagatgat tcaatgttct aagagtattt aatcggatgt tagtcgcaaa ttttcttaac
     1741 ccatcttctc tatttttact taaaatcatc ccttattcct cctttgcaga tggcttttta
     1801 ataaaggata ctccaaacat aactgctaga ataagaacaa gaccaatcac aatgcctgct
     1861 tgtgagccaa attgatttaa cattcctaaa ataattcctg atagaccaaa atctgcatct
     1921 gagaaagttg atccttggaa accaagtcct cccaaaactg gcattaaaaa gactggaaga
     1981 aaactgatta aaataccttg taaaaatgct ccaatagtgg ctccacgaac accaccagat
     2041 gcattcccaa tgacacctgc agtcgctcca cagaagaaat gaggcacaac acctggtaag
     2101 ataacaaccg ttcctgaagc aatcataatt gccatactta ctaaaccacc aacaaaacta
     2161 gagataaatc caattagaac tgcattgggt gcataagtat aaacaatcgg acaatccaaa
     2221 gcaggttttg aattaggtac aagacgctct gaaatacctt taaaggctgg aacaatttca
     2281 cccaaaataa ggcgaacacc tgctaaaata acaaataccc ctgctgcaaa ttgacctgct
     2341 aattgtaaag cataaactag accacttgta ccactactga tttctttttc tatatattct
     2401 gaccctgcaa agatagctac aataatgtaa ataactgcca tggataaagt gatactaaca
     2461 gtactatcac gtaaaaaagc taaactcttt ggaaatttaa tgtcctctgt tgattttgat
     2521 ttgtcaccga taaggctacc agtaaaacca ctcaaccaat atcccaaaga actgaaatga
     2581 cctaaagcta ccttgtcatt tccagttaat tgaaccatat atttttgcac aaatgctggg
     2641 gaaatactca taataatacc gagtgctaat cctcctagta agatgagagg caagctagta
     2701 aagccagcaa ctgataaaat gaccgcaatc atacatgcca tatatagagt gtggtgccct
     2761 gttaaaaaaa tatatttaaa tcgagtaaaa cgagcgatta agatattgaa caccatgcct
     2821 gcaaacataa tcattgcagt agctgagcca tatgttgtta aagctacagc tacaattgct
     2881 tcattattcg gcacaacgcc agataaatga aaagcatgct caaacatggt accaaatgga
     2941 tttaaagaat tttgtacaat tcctgcacca ccagatacaa ctaagaaacc aacaaaggtc
     3001 ttaattccac ctttaataat atcaggtaat ttcttcttct gaagaactaa tcctaagatt
     3061 gcaattaaag ctactaaaat agctggtgta ctaacaatat ccaatatgaa cttcatcatg
     3121 acgctagcct cctatataag tcctttttct tcacaaagtt tagtaattaa ttctcgtagt
     3181 tcatccatat caataatact atttaagata cgaacatctc caagatgact agctgaatca
     3241 gctagatcac gaccaacaat ccaaatatca gctgcatttg gatctgctcc acctaaatca
     3301 taatgttcaa cttctacatc cgaaacattc aaatcactca atacagattc aatattcatc
     3361 tgtaccataa aacttgaacc taatcctgaa ccacaagctg taccaatttt taacattatc
     3421 taatcctcct gtttaattat cattttaatg tcatcatagt tttttgatga tattaaagtt
     3481 tgaacatgat ttttatctct taaaattgtt gttaaatgtg acaaagcctt taaatgactc
     3541 tcattatcaa tggctgcaat acaaatcaac aatcttacct cttgttctgg attatccaat
     3601 aaataaatcg gttcttccaa aactaacatt gacattccta tttcattcac accttcatct
     3661 ggccgagcgt gaggaattgc tactcccttc cctaaattaa taaaaggccc aaactcttct
     3721 actttttgaa tcattgcctc agggtagttc tcagttatct tatcttgatc caaaagcggt
     3781 ttagctgcta aacgaatcgc ctccttccat cctaattttt gcgaactaac ctgataggtt
     3841 tctttggtaa taagttgttc tagcactggt acaatttcct ttctatcatt tttttgataa
     3901 agataattct ttaacgccaa tcttaattcc aattcttgtg taataattcc atatcttttg
     3961 acaatattca ggatttgttc aatctcaaaa tccccatact ctaaattcgg aaaatctttt
     4021 aacactagtt ctactagttg tattgcttgc tcttcagtca tcataaccga aactagataa
     4081 tttggctttt ctgtctccac ctttatggta gaaaaaacca tatcatagtc actactagct
     4141 ttcacctgta aatcatcaat ctttgaggtt cctataaact caatttgagg aaataatgct
     4201 aatagattct cttttaacat caatgaagaa ctaacaccat taggacaaat gattgctgct
     4261 ttataccatt tttgaggcaa agtatctgct ttctttaaat aacctccgaa atggataaca
     4321 aaatatgctg tttcactatc aggtatggga ttgtcaatag cgtccatcaa gggcatcaaa
     4381 gaatctttga ctaattcaaa taaatcagga taatgttctt taacatgcaa tacatattca
     4441 tttgaactag gtaggccgaa ctttaatcta tagtaagccg gtataaggtg gcgacgaaga
     4501 ttttctctca atccttccct ttgtttaaaa tgtaacaaag aaatatcttc cattctactt
     4561 ataatagcct ctgttaattg attaaagtaa accggagcaa catctacttc accttcaaag
     4621 caacttgata ataaaactgt gatatagcga taatcatcct cagaaaacac cgtatctata
     4681 attcccaaat caaccactgt atccaataaa atagtcgtta tatcttgaat aacaggagat
     4741 actaatgtct ctgaaagaca tactctttca acatcccttt gatacctaca cagaatgaat
     4801 actaaaccga aaaggtaaac ttttaattga ttaacaatag gtactagctg tagcttctca
     4861 taataatctt taactacctg atcaatcaaa tcataagtta atgaataccc ccaactggat
     4921 aaaacataat ccaaacccca aatccctatg gaggattcca gcaactcact aaccatttga
     4981 aaagctaagc ggtgcttatt ccactctgaa ccgtgtaaag tataaccttt tgctctactg
     5041 taccctagct ccaaatcatt atctaacata atctttctta atgattgaat atcagataag
     5101 gttgtattct tacttacttt caaaaagtct tggtaatgat tattcgatat aaaatctaat
     5161 cggcaaaaag tgtaaagata gattaaagct aagcgagtcg actttggtaa aaccaattca
     5221 tccgacttaa taatatctgt caaagactgc ttcgtacgat ttgataaact atagcgacct
     5281 tgctttttat ccagcactat ccctttatta gctaaataag gcactaaata atctattcct
     5341 tctttgactt cctttatagg taagctcacc ttaacagata actcatataa tgatagctca
     5401 caatgatcca tcaaagtcat caaaataact agtgctctat aatcaaacat ttctcttcct
     5461 ttctaactac aa
//
LOCUS       DAVMEI010000112         5468 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVMEI010000112 DAVMEI010000000
VERSION     DAVMEI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.112"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            78..566
                     /locus_tag="VYQ24_001561"
                     /pseudo
     CDS             78..566
                     /locus_tag="VYQ24_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809258.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            582..1062
                     /locus_tag="VYQ24_001562"
                     /pseudo
     CDS             582..1062
                     /locus_tag="VYQ24_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1448..1657
                     /locus_tag="VYQ24_001563"
     CDS             1448..1657
                     /locus_tag="VYQ24_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968684.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1647..2135
                     /locus_tag="VYQ24_001564"
     CDS             1647..2135
                     /locus_tag="VYQ24_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968685.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            2251..3711
                     /locus_tag="VYQ24_001565"
     CDS             2251..3711
                     /locus_tag="VYQ24_001565"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1968686.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3708..4532
                     /gene="nadE"
                     /locus_tag="VYQ24_001566"
     CDS             3708..4532
                     /gene="nadE"
                     /locus_tag="VYQ24_001566"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1968687.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4882..5433
                     /locus_tag="VYQ24_001567"
     CDS             4882..5433
                     /locus_tag="VYQ24_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968688.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
BASE COUNT         1673 a          954 c         1189 g         1652 t
ORIGIN      
        1 atcaagaaag atttagatgt ttgatccaag agttagattt gactcagtct attaataaaa
       61 aagtaaagac ctattctttg ggtacaaaac aaaaattagc tttgcttcta actctcgtta
      121 cggaacctga tatattgatt ttagatgaac cgactaatgg tttagatatt gaatcatcac
      181 aaatagtttt agcggttcta aaaaaattag ctttacatga aaatgtggga attttaatat
      241 cgagtcataa attagaagac attgaagaaa tttgtgagag agttcttttc ttggagaacg
      301 ggcttttgac atttcaaaaa gtaggaaaag atagtcataa tttcttgttt gagatagctt
      361 tttcatcagc tacagataga gacattttca ttaccaaaca agaattttgg gatattgttt
      421 aggaagaggg attgagaatt actatgtctg ggaatattca aaatagtgag ctttttaaat
      481 tttttaacga aaactctatt aaagtagttg attttgaaac taaaaaagag acgcttaaag
      541 atatttacct aaatcgttca aaataaagga aggttataat catgaaatta aataaacaga
      601 agaatcggat gatttacgtc ttgtctaatt ttctatatgc tatctcagtt tccattattt
      661 atgctttgaa tggcattgtg ttactagtca tagtaagtaa attgggtatt ccaggtgatt
      721 taggattaaa ttttatagta gctattgtag tcaatacaat tttgttagtc ctgttttatt
      781 ttctattatc ttacattttc tatttataca aattgaaaag tggcttggta tttggtattt
      841 tagtagcttt actactcttt atctctaata tattaaatac gatgatgatg aatactagta
      901 atgatttgtt tatcaaagca attgaacttc ttcctttttt attctttacc tgtatttgtg
      961 gcttcaaata cgatgtctat tagtcagtat cttgtgctaa tcactacaat cattttattg
     1021 tactttttca ctctcaagaa aagcaagaag tattcatttt agtttcattt agtattattt
     1081 tgcataccta aaatacaata aaaaaatcag tcatcttggt atgctcctgc tttcactatt
     1141 caacacgttt ttgacttata ctaggctcat ttccaaaagc attatataat agtgatatga
     1201 aaccaactaa actaaacaag aaatataagc aataaaaact cgtttaaaag atcctactaa
     1261 agctaatact aaataaaaat aaaagagtaa actaggaagt ttatttcaaa caacctaaaa
     1321 tactgatttt cggctgaaga taatactgga gtgcaaatta atggggttat aataaatagc
     1381 tgatagcttg tgttggtttt ggatttttta agagtagatg agtattaaaa ctataaggag
     1441 gacgaaggtg gctaaaaatt taaaattaaa attagctcgg gtagagcgtg atttaacaca
     1501 aggtcaactg gcagaggctg tcggggtgac gcgccagact attggtttga tagaagcggg
     1561 gaaatacaat cccagtctct cgctctgcca gtccatttgc agatgtttag ggaaaaccct
     1621 agaccaacta ttttgggagg aagaagatga aaaatagatt ttattattat caattactag
     1681 acgaaagaga agaacaactg ttcaataaag cgggctctga aagtttctat atctgcatcg
     1741 ctttgtcgct cctatcttat atcatttcag tattagcacc aagccttttt aattctaata
     1801 tgctgctaat cgttatcatc atagggacat tttacttttt caatcgtgcc cgttatctgg
     1861 gagtgaccta ctatagtcgt tttcatttta cgattttggg ttgttttttc ctaaccttgg
     1921 ctattacggc tcttttgatg ttgcagaatt atcaattcaa catagaaatt tatcagcaca
     1981 atcctttgaa ttttaaatac ctgtctgctt gggtcattac ttatatcatt taccttccgt
     2041 ggatctttat tggcaatctt ggtcttaaga gctatggcga atgggctcag aaaaaatttg
     2101 aacaagatat ggatgaattg gagagtggag aatagcttgt tactcttttc tcaatccagc
     2161 taaaatgtga tataatagta ctaatttatt ggaatacatg aaagttcttg aaaattttca
     2221 tgggtttcta gctaaggaag taggaaaagt atgtatccag atgatagttt gacattgcac
     2281 acggacttgt accagatcaa catgatgcag gtttactttg accaagggat tcacaataag
     2341 aaggcggtct ttgaggtgta tttccgccaa cagcctttta agaacggcta tgcggttttt
     2401 gcaggtttgg aaagaattgt gaactatctt gaagacttgc gtttttcaga tagtgatata
     2461 gcctatttgg agtcgcttgg ttatcatggg gcgttcttgg attaccttcg caatttcaag
     2521 ttggagttga ccgttcgttc tgcccaagaa ggggatttgg tttttgctaa tgaaccgatt
     2581 gtgcaggtgg aaggacctct agcccaatgt cagttggtcg aaacggctct tttgaacatc
     2641 gtcaactacc agaccttggt ggcgacgaag gcagctcgta ttcgttcggt tatcgaagat
     2701 gaacccttga tggagtttgg gacacgtcgg gctcaagaaa tggatgcggc catctgggga
     2761 acacgcgcag ctgtgattgg tggcgccaat ggaactagca acgtgcgtgc gggtaagctc
     2821 tttgacattc ctgttttggg aacccatgcc catgccttgg tacaggttta tggcaatgac
     2881 tatgaagctt tcaaggctta cgctgcgacc cacaaaaatt gtgtctttct tgtggatacc
     2941 tatgacaccc ttcgcatcgg tgtaccagct gccattcagg tggcgcgtga gctgggtgat
     3001 cagattaact ttatgggtgt gcggattgac tctggggata ttgcctacat ttctaagaaa
     3061 gtccgtcagc aactggatga ggctggattt acagaggcta agatttatgc ttctaatgat
     3121 ctagatgaaa ataccatcct taacctcaag atgcaaaagg ccaagattga tgtctggggt
     3181 gtgggtacca agctgattac agcctatgac cagccggctc ttggggcggt ttacaagatt
     3241 gttgcaatcg aagatgaaac tggtcagatg cgcaatacga ttaagctgtc taataatgct
     3301 gaaaaagttt ctacgccagg taagaagcag gtgtggcgca ttaccagtcg tgaaaaaggc
     3361 aagtcagaag gcgactatat cacttatgat ggtgtggata ttagcgacat gacagaaatc
     3421 aagatgttcc atccgaccta tacatacatc aagaagacgg ttcgtaattt tgatgccgtt
     3481 cctctcttgg tggatatctt caaagaagga atattagttt acaacttgcc tagtttgact
     3541 gacattcagg attatgcccg taaggaattt gacaagttgt gggatgagta taagcgtgtg
     3601 ctcaatccgc agcactatcc agtggatttg gcgcgtgatg tatggcaaga taagatggac
     3661 ttgattgata agatgcgcaa ggaagccctt ggtgaaggag aagaagaatg agtttgcaag
     3721 aaacgattat ccaagagctg ggtgtcaaac cagtgattga tgcccaggaa gaaatccgtc
     3781 gttctattga tttcttaaaa agatatctga aaaaacatcc cttcctaaaa acctttgtac
     3841 tagggatttc tgggggacaa gactcaacct tggcaggacg tttggcgcaa ttagctatgg
     3901 aagaactgcg agctgaaacg ggagacgata gctacaaatt tatcgctgtc cgcctgccat
     3961 acggagtgca agctgatgaa gcagatgctc aaaaagccct agccttcatc cagccagatg
     4021 tcagcttggt tgtgaatatc aaggaatcag ctgatgccat gacagctgca gttgaagcga
     4081 caggtagtcc tgtttcagac ttcaacaagg ggaatatcaa ggcacgttgc cgtatgattg
     4141 ctcagtatgc ccttgctggt tcccatagcg gagcggtcat tggaacagac cacgccgcgg
     4201 aaaatatcac aggtttcttt accaagtttg gtgacggcgg tgcggatatt ctccctcttt
     4261 accgcctcaa taaacgccaa ggaaaacagc tcttgcagaa acttggcgca gagccagccc
     4321 tttatgaaaa aatcccaacg gcagacctag aagaagataa accaggccta gctgacgaag
     4381 tcgcacttgg agtcacctac gcagagattg acgactacct agaaggcaaa acaatcagcc
     4441 cagaagctca agcgaccatt gaaaactggt ggcacaaagg ccaacacaaa cgccacttac
     4501 ccatcaccgt atttgatgac ttttgggagt aaaaaggtcc gggggacctt tttagcttct
     4561 tgccctgaaa ttaaaaagca agaaaaacct ccactggagg ttttcagcct ctcatcttga
     4621 aataagaaag tgagagaagg tctgggggat cttgaacccc gagtttagaa ataagaaaat
     4681 gaggcagatt cagtaactcg aagagttcga tttcatcgtc ttacccctgc aacgatgact
     4741 aggtttgaaa aagcttgcta gagcgcattt caaaccaggc agcaactgcg tcaagaaatt
     4801 agaagacaaa ctcgttttct agctgttact gagttgagcc tttttactac gagtatagaa
     4861 ataaggaagt gaggtagcat catgaaatct atcggtacgc aaatattaca gacagaacgt
     4921 ttgattttaa gaagatttgt ggagagtgat gcagaagcca tgtttcaaaa ttgggcttca
     4981 tccgctgaga atctgaccta tgttacctgg gatccccatc ctgatgtcga aatcactcga
     5041 aactcgattt gcaattgggt tgcttcctat actaatctca actattataa atgggccatt
     5101 tgtctaaaag aaaacccaga gcaagtaata ggagatatca gcattgttaa gatagacgag
     5161 gctgatttaa gctgtgaaat tggctatgtg ttaggcaagg cttactgggg aaatggtatg
     5221 atgacagaga ctttgaaagc tatcttggac ttttgtttta ctcaagcagg ttttcaaaag
     5281 gtcagagcac gttatgccag tctcaaccca gcttcaggtc gtgtcatgga aaaggctgga
     5341 atgtcctatc tacaaaccat tgttaatggt gtagagagaa aaggctatct tgcggatctt
     5401 atttattatg gtataagtag ggaagaatgt tgaattctat tttctgtttc tatcgaagtc
     5461 aactattt
//
LOCUS       DAVMEI010000113         5459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVMEI010000113 DAVMEI010000000
VERSION     DAVMEI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.113"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            17..1903
                     /locus_tag="VYQ24_001568"
     CDS             17..1903
                     /locus_tag="VYQ24_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HEV1968689.1"
                     /translation="MTIDISEESLAKESADLLKILLKDRTTKKSIVWATHSYELLGKG
                     FAPSDRINPSKVTGNFANLIQPRSEKSKYEQKDRTKIRAEVFTPTWLVAKQNGYVESK
                     LGSLSLEEYVDLRWLEVTCGEAPYMVTRYDTVTGEEIPLSERVGFVDRKLQRISREVS
                     DEVTFYKLVKDAYHASYGYEYQGDSLLLARENLLATFEDYYLAKTGNQPTLEQKKEIA
                     TIISYNVFQMDGLKKSSPYSAKQKQSQQLSLFADELEVQEVEESKTHIKDWKKNRMIG
                     FERLSSEESEMKFDVVIGNPPYQETGEARDEPIYHYFIDEAYKIADKSILITPARFLF
                     KAGQTPKNWMEKMLEDKHLKVQFYELKSGKVFTGTDIKGGVAITYRDVDKDLGPIGVF
                     TIFETLNGIVKKIEKHPEFETFSDLVYPQGIYRFSNKLFSDYPNAEKLQGKGTKNKIV
                     SKSLTQLDFVFKNNEEKDHVALLGRIGSERVYRYINKKYLDLPETFENYNVFVPEANG
                     SGALGEVLSTPLIGEPLIGEPLIGHTDTFLSIGNFKTKFEADACIKFIKTKFARVLLG
                     VLKVTQHNSRKTWYYVPLQDFTVNSDIDWTQSVTDIDRQLDQKYDLSPEEIAFIENHV
                     REMD"
     gene            2068..5322
                     /locus_tag="VYQ24_001569"
     CDS             2068..5322
                     /locus_tag="VYQ24_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1968690.1"
                     /translation="MPVEIKTTKEIHPKIYAYTTPTVTSNEGWIKIGYTERDVTQRIK
                     EQTHTAHIATDVLWTGDAAYTEEPDKGKTFKDHDFHHFLSFHDVERRPKTEWFYFNGT
                     PEKSKNLFDKFVQHDLSGYQPGKGQDYTLRQEQEEAVAKTLAYFQEHAGGKFLWNAKP
                     RFGKTLSTYDLARRMEAVNVLIVTNRPAIANSWYDDFETFIAGQTTYKFVSESDSLKS
                     RPILSRQEFLGILADDVRQLAFISLQDLKGSVYLGGEHDKLKWVTDLHWDLLVIDEAH
                     EGVDTFKTDQAFNKIRRNFTLHLSGTSFKALAKGDFTEEQIYNWSYADEQAAKYSWSL
                     EQEEENPYESLPQLNLFTYQMSQMIGEKLEKGAQIDGENIDYVFDLSEFFATDDKGKF
                     IHEHDVRNWLDTLSSNEKYPFSTKELRNELKHTFWLLERVASAKALKALLEEHPIYEN
                     YEIVLAAGDGRMSEEDDKVKLKSLDLVRKAIAENDKTITLSVGQLTTGVTIPEWTGVL
                     MLSNLKSPALYMQAAFRAQNPYSWSDNKGNHFRKERAYVFDFAPERTLILFDEFANNL
                     SLVTAAGRGTSATREENIRELLNFFPIIAEDRAGKMVEIDAKAVLTTPRQIKAREVLK
                     RGFMSNLLFDNISGIFQASQTVLDILNELPVEKEGKVQDSSDLLDFSDVTVDDEGNAV
                     VDHEIVVNQQMRLFGEKVYGLGESVAELVTKDEERTQKQLVNDLSKTVSSVIVEELKA
                     DYSLKTRETEQIKKQITATLENEIRKNDIERKISEAHIKQELQQQLKEANDKAQKDKI
                     QEDLEKRLEENKLIHKEKLEQTLKKEVEKMPEKFIEQVEIKRVEQLKQSAQDEIRDHL
                     RGFARTIPSFIMAYGDQTLTLDNFDAFVPEHVFYEVTGITIDQFRYLRDGGQDFAGHL
                     FDKATFDEAIQEFLRKKKELADYFKDQKEDIFDYIPPQKTNQIFTPKRVVKRMVDDLE
                     KENPGIFDDPSKTFIDLYMKSGLYIAELVKRLYNSNGLKEAFPNPEERLKHILEKQVY
                     GFAPSEIIYNISTNFIFGNLSKDISRKNFVLADTIPAAKEGSIQKLVDSYFENN"
BASE COUNT         1888 a          884 c         1113 g         1574 t
ORIGIN      
        1 ataagagagg ttgctgatga caatagatat tagtgaagaa agtttggcta aagaatcggc
       61 tgacttatta aaaattcttt taaaagatcg aacgacaaag aaatcaattg tttgggctac
      121 gcattcttat gaattgttag gaaagggatt tgctccaagt gatcgcatca atccaagtaa
      181 ggttactgga aactttgcca acttgattca gcccagatct gaaaaatcca aatatgaaca
      241 aaaagatcgg acaaaaataa gggcagaagt gtttacacca acgtggttgg tcgcaaagca
      301 aaatggatat gtagaatcga aattaggctc gttaagtctt gaggagtatg tggacttacg
      361 atggttagag gtaacttgtg gtgaagcacc ttatatggta acgagatatg ataccgtaac
      421 tggtgaggaa attccattat ctgagcgagt tggttttgtt gatagaaaac tgcaacggat
      481 tagtcgtgag gtttcagatg aagtcacatt ttataaactt gtaaaagatg cttatcatgc
      541 ttcgtatggt tatgagtatc aaggagattc tctccttttg gcgcgtgaga atcttttggc
      601 aacttttgag gactattatc tagctaagac tggaaatcaa ccaacattag agcaaaagaa
      661 agaaattgcg actattatct cttacaatgt ctttcagatg gatggattga aaaaaagtag
      721 tccttattca gctaaacaaa agcaatctca acaattgagt ttgtttgcgg atgagctaga
      781 agtccaagaa gtagaagaat ctaaaaccca tatcaaagac tggaaaaaga atagaatgat
      841 aggttttgag cgcctatcta gcgaggaaag tgaaatgaaa tttgatgtcg tgataggaaa
      901 tcctccgtat caggaaacag gagaagccag agatgagccg atatatcact attttattga
      961 tgaagcttat aaaatcgcag ataaatctat actaatcacc ccagcaaggt ttttatttaa
     1021 agctggtcaa actcctaaaa attggatgga aaagatgctt gaagacaagc atttaaaagt
     1081 tcaattctat gaacttaaaa gcggtaaagt atttacagga acggatatta agggtggagt
     1141 agcaataact tatcgtgatg ttgataaaga tttgggacca atcggagtat ttactatttt
     1201 tgaaactttg aatggtattg taaaaaaaat cgaaaaacat ccagaatttg aaactttttc
     1261 tgatttagtt tatcctcaag gtatttatag attttcaaat aaactttttt ctgattatcc
     1321 aaatgctgaa aagctgcaag gaaaagggac aaaaaataaa attgtttcga aatcactaac
     1381 tcagttagat tttgttttca aaaacaacga agaaaaagac catgttgctc tacttggaag
     1441 aattggctcc gaacgtgttt atcgatatat taataaaaaa tatttagatt taccggaaac
     1501 attcgaaaat tataatgttt ttgtaccaga agctaatgga agtggtgcct taggtgaagt
     1561 cttatcaaca cccctaatcg gggaacccct aatcggggaa cccctaatcg ggcatacaga
     1621 tactttttta tctattggta attttaaaac aaaatttgaa gccgatgctt gtattaaatt
     1681 tattaaaact aaattcgcta gagtattatt aggtgttttg aaagttactc agcataattc
     1741 acgcaaaact tggtattacg tccccctcca agactttacg gtcaattcgg acattgattg
     1801 gacacaatca gtgactgata ttgaccgcca gcttgatcaa aaatatgacc tttcccctga
     1861 agaaattgcc tttattgaga atcatgtaag ggagatggat tagaaaagta tttttatttg
     1921 acaaatagtg ctcaatgatc taaaatgact atataggatt aggtcaggaa gcatacgatg
     1981 ccctgaccct ttttgtactt atgagatgag aaagtcattt gttagataaa ttgacttcgt
     2041 tagcaaacgt tcaaaaaagg aaaacttatg ccagtagaaa ttaaaaccac taaagaaatt
     2101 catcctaaaa tctatgccta caccacaccg acagtaacca gtaatgaagg ctggattaag
     2161 attgggtata cagaacgtga tgtcacacaa cgtatcaagg agcaaacgca tacagctcat
     2221 atagctacag atgtcttatg gactggtgat gcagcttata cagaagagcc tgataagggg
     2281 aaaactttca aggaccatga tttccaccat ttcctttctt tccatgatgt agaacgtcgt
     2341 cccaagacgg aatggttcta ttttaatgga actcctgaaa aatcaaaaaa tctttttgat
     2401 aagtttgttc agcatgattt gtctggttat cagcctggaa aaggacagga ctatactctg
     2461 cgacaagagc aagaagaagc agttgctaag acattagctt atttccaaga acatgctgga
     2521 ggcaagtttc tctggaatgc caagccacgc tttggtaaaa ccttgtctac ctatgaccta
     2581 gctcgacgga tggaagctgt caatgtccta attgtaacaa accgccctgc cattgctaac
     2641 tcatggtatg atgattttga aacattcata gcaggtcaaa cgacttacaa gtttgtttct
     2701 gaatcagata gccttaagag tcgtccaatc ttgtcacgac aagaatttct tggtatttta
     2761 gctgacgatg taagacaact tgcttttatc agtctccaag acttgaaagg atctgtttat
     2821 ttaggtggag agcacgataa actcaaatgg gtaactgatc tgcattggga cttgttggtt
     2881 attgacgagg ctcatgaagg agttgatacc ttcaagactg accaagcctt taataagatt
     2941 cgacgaaatt ttactctgca tttgtcaggt acatcattta aagcattggc taaaggagat
     3001 tttacagagg aacaaatcta caactggtct tatgctgatg agcaggctgc taagtattcg
     3061 tggtctcttg agcaagaaga ggaaaatcct tatgaaagct tgcctcagtt gaatctcttt
     3121 acctatcaaa tgtctcagat gattggcgaa aagttagaaa aaggcgctca gatcgatggt
     3181 gaaaatattg actatgtttt tgacttaagt gaatttttcg ctacagatga taaagggaaa
     3241 tttattcatg agcatgatgt cagaaattgg ttagatactc tatcaagcaa tgaaaaatat
     3301 ccattttcaa ccaaagaact ccgtaatgaa ctcaagcata ctttttggct tttagaacgt
     3361 gtcgcttcgg ccaaagcatt aaaagcccta ctagaagaac acccaatcta tgaaaactat
     3421 gagatcgttc tagctgctgg tgacggacgt atgtccgaag aagacgataa agtcaaactc
     3481 aaatccttgg acttggttag aaaagcgata gcagagaatg acaaaaccat taccctatcc
     3541 gttggtcagc tgacgacagg tgtcactatc cctgaatgga caggtgtatt gatgttatca
     3601 aatttgaaat caccagctct ttatatgcag gccgccttcc gtgctcaaaa tccttactca
     3661 tggagcgata acaaaggaaa tcactttcgc aaagaaagag cctatgtatt tgactttgcg
     3721 ccggaaagaa ccttgattct ctttgatgag tttgccaaca acttatcgct tgtaactgca
     3781 gctggtagag gaacttcagc tacacgcgaa gaaaatatta gagaattatt aaacttcttt
     3841 ccaattattg ccgaagaccg tgctggtaag atggttgaaa ttgatgcaaa ggcagttcta
     3901 accactcctc gccagataaa agctagagaa gttcttaaac gaggttttat gtccaatctc
     3961 ttatttgata atattagtgg tattttccaa gcaagtcaaa cagttttaga tattttaaat
     4021 gagctgccag ttgaaaagga agggaaggta caagatagtt ctgatttatt agatttttca
     4081 gatgttacag tcgatgatga gggaaatgca gtagtagacc atgaaattgt agttaatcag
     4141 caaatgcgac tttttggtga aaaagtttat ggacttggtg aatctgttgc tgagttagtc
     4201 acaaaagatg aggaacgaac tcaaaaacag ctggtcaatg acttgagtaa gaccgtttct
     4261 tcagtgattg tagaggaatt gaaagcagat tattctctaa aaacaaggga aactgagcaa
     4321 attaagaaac aaattacagc aacacttgag aatgaaattc gaaaaaatga tatcgaaaga
     4381 aaaatttctg aagctcatat caagcaagag ttgcaacagc agctcaaaga agcaaatgat
     4441 aaagcgcaaa aagataagat tcaagaagat ttggaaaaac gtttagaaga aaataaactc
     4501 attcataaag aaaaactaga acaaacactc aaaaaagaag tggaaaaaat gcctgagaaa
     4561 tttatcgaac aggttgagat aaaacgtgtg gaacagttga aacaatcagc tcaagatgaa
     4621 attcgtgacc atttacgagg gtttgcaaga acaattccaa gttttattat ggcttacggt
     4681 gatcaaactc taacacttga taattttgat gcctttgttc ctgaacatgt tttttatgaa
     4741 gtaacaggga ttacgattga tcagtttaga tatttgcgag atggtgggca ggattttgca
     4801 gggcatctct ttgataaagc aacatttgac gaagctattc aagaatttct tcgcaagaaa
     4861 aaggagttgg cggattattt taaagatcaa aaagaagaca tttttgacta tattccaccg
     4921 cagaagacca accaaatttt cactcctaaa cgagtggtga aaaggatggt agatgatttg
     4981 gaaaaggaaa atccagggat ttttgatgat ccatctaaga cttttattga tttatatatg
     5041 aagtcaggcc tctatattgc agaacttgtg aagcggttat ataatagcaa tggcttgaaa
     5101 gaggcctttc caaatcctga agaacgctta aaacatattt tggaaaagca agtttatgga
     5161 tttgctccgt ctgagattat ctataacatt tccactaatt ttatatttgg taatctttct
     5221 aaagatatca gtaggaagaa ttttgtttta gcagatacca ttccagcggc taaagaaggg
     5281 agcattcaaa agttggttga ttcctatttt gaaaataatt aaaaagaagg ccgagtcaaa
     5341 attctttgaa atcagaaaaa acgcataata ttgagtgctt ttgtactgcc ccccaaaagt
     5401 tagacagaaa aaatctaact tttggggggc agttcagaca atccttggta ttatgcgtt
//
LOCUS       DAVMEI010000114         5443 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVMEI010000114 DAVMEI010000000
VERSION     DAVMEI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.114"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1047
                     /locus_tag="VYQ24_001570"
     CDS             <1..1047
                     /locus_tag="VYQ24_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1968691.1"
                     /translation="KIEDAKVVAVFDPNHGEEVAQELGSDVCASLDELVAREDIDCVI
                     VASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKAMVDACKENNVIFMAGHIMNFF
                     NGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSWKKLRSQSGGHLYHHIHELDCI
                     QFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLEYSDDRYAVLEYGNAFRWGEHY
                     VLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQEEDDDRTAIYTGRGMDGAIAY
                     GKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEKLLNGVAALESIATADACTLSV
                     KEDRKVSLSEITNA"
     gene            1204..2094
                     /locus_tag="VYQ24_001571"
     CDS             1204..2094
                     /locus_tag="VYQ24_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1968692.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            2119..2442
                     /locus_tag="VYQ24_001572"
     CDS             2119..2442
                     /locus_tag="VYQ24_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968693.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            2432..4423
                     /locus_tag="VYQ24_001573"
     CDS             2432..4423
                     /locus_tag="VYQ24_001573"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV1968694.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            4439..4915
                     /locus_tag="VYQ24_001574"
     CDS             4439..4915
                     /locus_tag="VYQ24_001574"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV1968695.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            4949..>5443
                     /locus_tag="VYQ24_001575"
     CDS             4949..>5443
                     /locus_tag="VYQ24_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968696.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESGLLI
                     SIGKI"
BASE COUNT         1866 a          730 c         1094 g         1753 t
ORIGIN      
        1 aaaattgaag atgcaaaagt ggttgcggta tttgacccaa atcatggaga agaagttgct
       61 caagagttgg gatcagatgt ttgtgcaagt ttagatgaac ttgtagcacg tgaagatatt
      121 gattgtgtga tcgtagcttc acctagctac cttcaccgtg aaccagttgt gaaagctgct
      181 caacatggca aacacgtatt ttgtgaaaag ccaattgcat tgtcttatga agattgtaaa
      241 gccatggttg acgcatgtaa agaaaataat gtcatcttta tggctggtca catcatgaac
      301 ttctttaacg gtgtacacca tgctaaagaa ttgattactc aaggtaaaat cggtaaagtt
      361 ctttattgcc atgctgctcg tacaggttgg gaagaacaac aaccaactgt atcatggaag
      421 aaacttcgtt ctcaatctgg aggacatttg taccaccata ttcatgaatt agattgcatt
      481 cagtttatca tgggaggact tcctgaaaaa gcgacaatgg taggaggcaa tgtatatcat
      541 aaaggtgaaa actttggtga tgaagatgat atgctcattg taaacttaga atactctgat
      601 gatcgttatg ctgttttgga atatggtaat gctttccgtt ggggtgaaca ctacgtcttg
      661 attcaaggaa ctgaaggagc tatcaaactt gacttgttca atactggcgg tactcttcgt
      721 gttaaaggtg aaggagaatc acacttctta gttcatgaaa ctcaagagga agatgatgat
      781 cgtacagcta tctataccgg tcgtggtatg gatggagcaa ttgcgtacgg taaaccagga
      841 gtacgttgcc cattatggtt gcaaacatgt attgataaag aaatggaata tctacatgac
      901 atcattaaag gtggagaaat tacagaagaa tttgaaaaac ttctcaatgg tgtagctgct
      961 ttagaatcaa tcgctaccgc tgatgcatgt actttatcag ttaaagaaga tcgaaaagta
     1021 agtctttcag aaatcacaaa tgcttaactt ttgtaaaaca gaatagtaaa ttcttgtcat
     1081 tatataattt ctaaagttct gtgatacaac tcattgaata aagaaataga gatgggactg
     1141 ggataatgcc cagtcccatt ttttatcaaa aagtaatgag atcaaaaatg tgggagtgtt
     1201 gaaatgaaga ttataggtat cgatattggc ggaacaacaa ttaaggcaga tttatacgat
     1261 gagtttggaa cgagtttgaa tcatttcaaa gagatagaaa caattattga ctatgatttg
     1321 ggaacgaatc agatattaaa tcaggtctgt gatttaattg gtgagtatac tttaaatcat
     1381 tcaattgatg gtgttgggat ttccactgct ggagttgtta atgctaatac tggagaaatc
     1441 atctatgcag gctatacaat accagggtat atcggagtaa actttactgc cgaaatagaa
     1501 aaacgttttg ggttgtatac ttttgttgaa aatgatgtta attgtgctgc attaggtgaa
     1561 ttgtggaagg gacaagccaa agataagaaa aatgtagtaa tggttactat tggaacaggt
     1621 ataggaggca gtattattgt caacggacaa attgttaacg gatttaacta tactgctggt
     1681 gaagtaggtt atattcctgt aggtaattcg gattggcaaa gtaaagcctc aacaaccgca
     1741 ttgattcatt tatatcaaaa aaagagcttg aaaactaatc aaactggacg tactttcttc
     1801 actgatttaa gatctggaga taaagttgct gaagaaactt ttgaaatttt tgtagaaaat
     1861 ctaacaaaag gtttattaac gatttcttat ctacttaatc cagaaattct catattagga
     1921 ggtgggattc tggatagtaa ggatattttg ttacctgaaa ttcaaagttc tttagctaaa
     1981 aatgcaatgg ataataggtt tttacctaaa aatcttgtgg cagctacatt aggaaatgaa
     2041 gctggtcgta taggagctgt aaaaaatttc ttagatagaa tttctaataa atagtatgta
     2101 agataaggag gtgtcacaat gactaactct gtattttcga caatgcaaga tattgagaat
     2161 gttgcaaccg atattataaa atcatatgat aatgagattt atacttataa agctgtttcc
     2221 caagaagaat tggaaaaact agaaaaaagt tatgatgaaa aaagtcacga agaattagtt
     2281 tcaatagaaa gcaatttaga aatgaaacaa cagaacctta ttgatgaggt taataaaaca
     2341 atcaaggaaa atgatgcaaa tattcagtat atttcatcaa gtaggagagg agaatttgta
     2401 gaaaaaatta ttggtagggt ggtagaaaaa tatggccatt agtcagatga aaagaatctc
     2461 tctactattt tctaaaagta gtcttgatga tgttttaaaa actattcaag aactagagtc
     2521 agtgcagttc cgtgatttaa aggttcagga taactggtca gaagctctag aaaaagatga
     2581 agttgtattt ccaactattc aaatttttca tacttctaat tccaatcatg gggttattga
     2641 gggaaatgat gccttgactt atttgatgaa tcaacaacaa catttagaag caactgtaga
     2701 gaaattacaa gaatacctac cgaaagaaaa cacgtttaaa ttattgcagc aacctccgat
     2761 aactacctct tatgaagaat tagagaaatt tggtaaagct aatgttgctg agggtgttct
     2821 taaaaaagtg aatcatcaaa ttaacagagt tcatgaatta gaaagacaca ttcaaagtaa
     2881 taatgaggaa atagagcgat taataaagtg ggaaaaatta gaaattgttc ctgcgaattt
     2941 agaacaattt tctttctgta aaggaaaagt cggaacaatt ccaaggactg aagataatcg
     3001 cttatacaat agtcttttag aaaacaatat tgaagttcaa gaaatatttt ctaatgatag
     3061 agagtacggt gttgttgttt tctatcagtc tagttactct atagattttg atgaatactt
     3121 atttgaacca tttgattatt ctagaaagga attaccgaag cagcgagtag tagatttaga
     3181 tcaagaaaac atgcagttaa taactgaaaa agagaatatt atcgcatcgt tgcaagattc
     3241 aaagaaatat ttgatagatt tacaatggca aatagactat attttatcta tctatgctcg
     3301 tcaaatctct aagaataact ttttgtgcac tccgcatcta gttgcattag aaggatggat
     3361 agaagaaact cgtattttat attttataaa agttatggat gagcattttg gacattctat
     3421 ttatatttat gaatcggaaa cattgacgga taatcaagat gaaataccta tcaaattaac
     3481 gaatcattct ttaattgaac catttgaatt attgacagaa atgtatgctc tgcccaaata
     3541 ttatgagaaa gatcctacac ctgtattagc accattttac tttacatttt ttggaatgat
     3601 ggttgctgat ttaggctatg gtttactatt gtttttagga acaatgttag cattaaaaat
     3661 ttttcatcta ccttcagcaa ctaagagatt tttaaaattc tttaatatat taggggtagc
     3721 cgttgcaatt tggggtggaa tctatggctc attttttgga tatgagttgc catttcatct
     3781 gatatctaca acctctgatg tcatgactat attagtagtg tcagttgtgt ttgggtttat
     3841 tacagtattt gcaggtttgt tagcttcagg actacaaaaa gtaagaatga agaaatatgc
     3901 agaagcatat aattcaggat ttgcgtggtg tgttattctg cttggcttgt tatttattgc
     3961 tgttggaatg ttgatgcctg atatgagacc gttatttgta ttagggaaat gggtatctat
     4021 ttttaatgct gtggggattt tgattgtttc tattattcaa accaaaagct tgtcaggtat
     4081 tggagcagga ttgtttaatc tatataacat ttcatcttat ataggtgatt tagttagttt
     4141 cactcgattg atggcattag gattatctgg agcaagtata gcatcagctt tcaatttaat
     4201 tgttggtttg tttccgggaa tattggctaa actgacaatt ggattagtat tattcattct
     4261 tttacatgcg atcaatattt ttctatcgtt actatcagga tatgttcatg gagcacgtct
     4321 gatatttgtt gaattttttg gtaagtttta tgagggtgga ggaaaaccat ttcaaccttt
     4381 gaaggcttct gagaaatata ttaaggttat tacaaagaat taatggagga tatatataat
     4441 ggaacattta gcaacttatt tttcaaccta tggaggagct ttcttcgctg cattgggaat
     4501 tgtattggcg gttggattaa gcggtatggg gtctgcttat ggagttggta aggctgggca
     4561 atctgccgca gctttactga aagaacagcc tgaaaagttt gcctcagctt tgatattgca
     4621 attattgccc ggaacacaag gattatatgg ttttgttatt ggaattttaa tttggttgca
     4681 attaactcca gaacttcctt tagaaaaagg cgttgcttat ttctttgtag ctcttccaat
     4741 tgctattgta ggatactttt cagctaagca tcaaggaaat gtagcagtag cgggaatgca
     4801 aatcttggct aaaagaccaa aagaattcat gaagggagca attttagctg ccatggtaga
     4861 aacctatgca attcttgctt ttgtcgtatc attcattttg acccttcgtg tataagaaat
     4921 aaatttgcaa ttcaaaggag gtgtctaaat gagcaattta gaaaacttac gagagtctgt
     4981 tattgaacaa gctcatgaaa aagggcgtat gaaattattg gattccaaaa agaagattga
     5041 tgatgaattt gaaatgcaaa agtcgctcat tataaagaaa aaagaagctg aacatgaacg
     5101 aaagttaaaa gaattgcaac agaaatatca aataattttt caacaattaa aaaataagga
     5161 acgccaatca acgttagtat caaaacagaa aatattaaaa gaactttttc aatctgcttt
     5221 actagaaatg gaatcttgga gtgcagataa agaaatggag ttcatctatc gaattctgga
     5281 acgatattca caacaagagg tcatagtaac ctttggggaa cggactttag ctaaattcaa
     5341 tttggaacaa ttagagaaat tgaaattctc ttttccaaat tatttattta gtgaacaacc
     5401 tatctcaaat gaatcaggct tacttatttc aataggtaaa att
//
LOCUS       DAVMEI010000115         5239 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVMEI010000115 DAVMEI010000000
VERSION     DAVMEI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.115"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            210..1976
                     /locus_tag="VYQ24_001576"
     CDS             210..1976
                     /locus_tag="VYQ24_001576"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1968697.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            2122..3273
                     /locus_tag="VYQ24_001577"
     CDS             2122..3273
                     /locus_tag="VYQ24_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1968698.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFRDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGRPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            3418..4302
                     /locus_tag="VYQ24_001578"
     CDS             3418..4302
                     /locus_tag="VYQ24_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1968699.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            4700..4951
                     /locus_tag="VYQ24_001579"
     CDS             4700..4951
                     /locus_tag="VYQ24_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1968700.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            4939..5229
                     /locus_tag="VYQ24_001580"
                     /pseudo
     CDS             4939..5229
                     /locus_tag="VYQ24_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1657 a          903 c         1156 g         1523 t
ORIGIN      
        1 catgaagttt ttaaacaaca tattgacagt ttagaaagca ataatctatc catagactta
       61 aaagggctga agggaaaaaa ggttagaatt tctttgagaa acgcaggaat tcctttaagt
      121 ttggcagagg tagaggttta tacttataag taattttaaa aattatcatc ggtagtatga
      181 aagaaacaga aaaataagag gaaaatagta tgattcaaca tccacgtatt gggattcgtc
      241 cgactattga tggtcgtcgt caaggtgtac gcgaatcact tgaagtacaa acaatgaaca
      301 tggctaaaag tgtggcagat ttgatttcaa gcacattgaa atatccagat ggggaacctg
      361 tggaatgtgt gatttctcca tctaccattg gtcgtgttcc agaggctgca gcttcccatg
      421 agttgtttaa aaaatcaaat gtttgcgcaa caattacagt tacaccatgc tggtgttatg
      481 gtagtgaaac tatggatatg tctccagata ttcctcatgc tatttgggga tttaatggga
      541 cagaacgccc aggagctgtc tatcttgcag ctgtactagc ttcacatgct caaaaaggga
      601 ttccagcctt tgggatttat ggtagagatg ttcaggaagc taatgataca gatattccag
      661 aagatgtcaa agaaaaactt ttacgttatg cgcgtgcagc tcttgcaact ggcttgatga
      721 gagacactgc ttacctatca atgggtagtg tttcgatggg gattggcggt tctattgtaa
      781 atccagattt cttccaagaa tacttaggaa tgcgaaatga atcggtagat atgacggagt
      841 tcacgcgccg tatggaccgt ggtatttatg acccagaaga gttcgaacgt gctatggttt
      901 gggtaaaaga acatatcaaa gaaggggttg accgaaatcg tgaggatttg attctttcta
      961 aagaagagaa agaaaaacaa tgggaatttg ttattaagat gttcatgatt ggacgtgact
     1021 taatggttgg taacccaaga cttgctgaac ttggttttga ggaagaagcg gttggtcacc
     1081 atgctttagt agctggtttc caaggtcaac gtcagtggac agaccatttt ccaaatggag
     1141 attttatgga aactttcctc aatactcagt ttgactggaa tggtattcga aaaccatttg
     1201 tatttgcgac agagaatgat tcactaaatg gtgtgtctat gctctttaat tatctattaa
     1261 caaatactcc acaaatcttt gctgatgtgc gtacttattg gagcccagag gctgttaaac
     1321 gtgtaacggg acatacttta gagggtcgtg ctgcagctgg cttcttacat ctaatcaact
     1381 ctggttcttg tacattggat ggtacaggtc aagctactcg agatggcaaa cctgttatga
     1441 aaccattctg ggagttggaa gaaagtgaag tgcaggctat gcttgaaaat acagacttcc
     1501 caccagcaaa ccgcgaatac ttccgtggag gaggattctc aactcgtttc ttgacgaagg
     1561 gggatatgcc agtaacaatg gtacgtctca atcttctaaa aggggttggt ccagtgctac
     1621 aaattgcaga aggttataca cttgaacttc ctgaagatgt tcaccatact ttagataatc
     1681 gtacagatcc aggatggcca actacttggt ttgctccacg tttgacagga aaaggtgctt
     1741 tcaagtctgt ctatgacgtc atgaataatt ggggagctaa tcacggagcc ataacatatg
     1801 gacacattgg agcagacttg attaccttgg cttctatgtt gagaattcct gtcaatatgc
     1861 ataatgtacc tgaggaagat atctttagac ctaaaaattg gtccttattt ggaacagaag
     1921 atctagaatc agcagactat cgtgcatgtc agttgttggg accactacat aaataaaact
     1981 tgtttatata ggaggtgaac ttacgtccct cctatccttt taaaaagatt tgttaaacaa
     2041 ttcacaaata attgaaaacg aatacaaaaa gtaatataat gatgttaaat agatagcgca
     2101 aaggcgcagg aggaaaatta tatggctaca ttttatgttc cggcagtcaa ccttattggt
     2161 aaaggtgttg taaatgaagt gggtccttat atcaaggaac ttggctataa aaaggcactt
     2221 ttggtgacag ataagtacat cgaaggcagt gacattttac ctaaggtttt aaaaccgcta
     2281 gatacagaag gaatcgaata tgtcatcttt agggatgtag agccaaaccc tacttgtaaa
     2341 aatgtcacag atggggtagc tgctttgcaa gaacatggat gtgattttat catcagtctt
     2401 ggtgggggtt ctccacagga tgcagctagt tgtatttcta tcatggctac aaatggtgga
     2461 agaccacagg attatgaagg gcttcataag tctgctaaaa aaggcttgcc agttgtggct
     2521 atcaatacaa cggcaggaac ttctgcagaa atcactatta actatgtgat tactgatgaa
     2581 gaacgcaagg ttaagatggt aatggttgac aagaatagcc ttgctcttat ctctgtcaat
     2641 gacccggaac tcatgctttc caaacctcaa gccttgactg ctgctactgg tatggatgct
     2701 ctgactcacg ctgttgaagc tttggtaaca cctggtgctt atgatgtaac caagaaactg
     2761 tctattggtg ctattgagct tatcaaggaa tatcttcctc gtgctgtaga aaatggacat
     2821 gatattgaag cgcgtgaagg tatggtcaat gccatcttcc ttggtggtat gagctttaat
     2881 aatgctggtc ttggttatgt tcactcaatg gctcaccaac tcggtgcagt atataattta
     2941 ccacatggtg tttgctgtgc catgttgcta ccagttatag aacgtgaaaa tgctaaacgt
     3001 gtaccagaag ctttccgcaa tgttgccaaa gccttgggac ttcatgtaga aggtaaatca
     3061 gatcaagaat gtgccgatta tgcgattgct gagattgaga agctttctga gacagtaggt
     3121 attcctaaga aactgactga acttggtatt gaagaaaaag atttcgactt tgaatacctt
     3181 tctaagaatg ccttgattga tgcctgcgca ccaggaaatc catttatgcc aaccttagaa
     3241 gaaacgattg ccttttataa agagttattt tagcaagaga ttggacgaca ataggacaat
     3301 tcacctctaa ttattgacat ttcaggaaaa atcgcagtaa aaacggcaca gccttcttta
     3361 aatgtgctat aatactagaa aaatacttgt ggaggttcca ttatggcaat atttttcatg
     3421 atttttctga ttgtttgtgt gctcctattg gtgatagtca cactgagtac agtttatgtg
     3481 gttcgtcagc agtcggtggc gattattgaa cgctttggga aataccaaaa ggttgctaat
     3541 agcggtattc atattcgctt gccttttggg attgactcga ttgcagcacg gattcagttg
     3601 cgcttgttgc aaagtgatat tgtggttgag actaagacca aggacaatgt gttcgttatg
     3661 atgaatgtag cgactcagta ccgtgtcaac gagcagagcg tgacagatgc ttactataaa
     3721 ctcatacgtc cagaatctca gattaaatct tatatcgaag atgctcttcg ctcttctgtt
     3781 ccaaaattaa ccttggatga attgtttgag aaaaaagatg agattgccct tgaagttcaa
     3841 caccaagtag cagaagaaat gaccacttac ggctacatta tcgtgaaaac cttgattacc
     3901 aaagtcgaac cggatgcaga agttaagcaa tccatgaatg aaatcaatgc ggcgcaacgt
     3961 aagcgggtcg cagcacaaga attggcggaa gctgacaaga ttaaaattgt cactgcagct
     4021 gaagcagaag cagaaaaaga ccgccttcat ggtgtgggga ttgcccaaca acgtaaggcg
     4081 attgtggatg gattggcaga gtctatcacc gaactcaagg aagccaatgt tggcatgaca
     4141 gaagaacaaa tcatgtctat cctcttgacc aaccagtatt tggatacctt gaataccttt
     4201 gcctctaaag gaaatcaaac catcttttta ccaaatacgc caaatggtgt ggatgatatc
     4261 cgaacacaaa tcttgtcagc ccttcgcgct gagaagaaat aatagactaa tactcttcga
     4321 aaatctcttc aaaccacgtc agcgtcgcct tgccgtatat ttgagctgac ttcgtcagtt
     4381 ctatctgcaa cctcaaaaca gtgttttgag ctgacttcgt cagtcttatc tactacctca
     4441 aaacggtgtt ttgagcaacc tgtggctagt ttcctagttt gctatttgat tttcattgag
     4501 tacaaagagc ttggcaacag gctctttttg tattgcgttt attaagaaaa acaaaaaaca
     4561 tagatataca tttaactaaa aattattgta tgttacctcc cttaattagg aatgataagg
     4621 gaataactag aaagatttgt gaatacaaac tatttctgat atacaaaata tacagtaata
     4681 atgaatgatg ggagatggga tgaaagaatt tcaatttgag agaaagcagc gtttttcttt
     4741 gaggccatat gcaataggag cttgttcggt cttgctagga acgagtttat tttttgctgg
     4801 tatgggtgat cagcctgtac aggatacaga aacgagttca gcactaattt caagtcatta
     4861 tttggatgag caggatttat ctgaaaagct gaaatctgag ttgcaatggt ttgaattaga
     4921 aaacaagctt ttgaacttat gggagcatta ggttactatg aaggatttgt tccttatgtt
     4981 tcaaattaat acaaaaacca agctgaagaa gaagacaaac cgctatctga taaatatatt
     5041 tttgaaaaaa tcttaggaaa aacatatgca gccttcaaaa aagatcaaat taatgagcgt
     5101 gtcgagaaat taggtaagtt aaaacctatt acaataaatt acaacggaaa atcagaagta
     5161 attgatagta aagaaaaatt acaagagctt atgaataaag ccgttaaaga cgaagtggct
     5221 caaatatagt aaaatgaaa
//
LOCUS       DAVMEI010000116         5127 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVMEI010000116 DAVMEI010000000
VERSION     DAVMEI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.116"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            36..362
                     /locus_tag="VYQ24_001581"
     CDS             36..362
                     /locus_tag="VYQ24_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1968701.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            349..942
                     /locus_tag="VYQ24_001582"
     CDS             349..942
                     /locus_tag="VYQ24_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1968702.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            935..1846
                     /locus_tag="VYQ24_001583"
     CDS             935..1846
                     /locus_tag="VYQ24_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1968703.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTDNHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            1908..2972
                     /locus_tag="VYQ24_001584"
     CDS             1908..2972
                     /locus_tag="VYQ24_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968704.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKFETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(3150..4010)
                     /locus_tag="VYQ24_001585"
     CDS             complement(3150..4010)
                     /locus_tag="VYQ24_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1968705.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(4132..5118)
                     /locus_tag="VYQ24_001586"
     CDS             complement(4132..5118)
                     /locus_tag="VYQ24_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1968706.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
BASE COUNT         1516 a         1169 c          962 g         1480 t
ORIGIN      
        1 ataaaaatct tacacattag aaaaggaggt ttcccatgta ctttccaaca tcctctgcct
       61 tgattgaatt tctcatcttg gctgtactgg agcagggtga ttcttatggt tatgagatta
      121 gccaaaccat taagctgatc gctaatatca aagaatccac actctatccc attctcaaaa
      181 aattggaagg caatagcttt ctgacaacct attctagaga gttccaaggt cgcatgcgca
      241 aatactactc cttgacaaac ggtggtatag agcagctctt gaccctaaaa gatgaatggg
      301 cactctatac agacaccatc aatggcatca tagaagggag tatccgccat gacaagaact
      361 gaatacctga ctcagctaga actctatctc aagaaactac ctgaagctga ccgtatcgaa
      421 gccatggact atttcagaga gctctttgac gatgctggag tcgaaggaga agaagaactc
      481 atcgctagtt tgggaactcc caaagaagcg gcccacgaag ttctatccaa tcttctcgat
      541 aaaaaaatca atgaagcacc cgctcaaaaa aataaccgac aaattttaca tatcgccttg
      601 ttagccctcc ttgcagcacc tatcggcatt cctctgggaa tcgccatcct cgtgaccctg
      661 ttcgcaatcc ttgtagccgc tttgactgtc attctggctt tctttgcagt ttccatactg
      721 ggtatcatcg gcggattcct atttttagtt gaaagtttca ctatcctcgc ccaagccaaa
      781 tcagccttta tcttgatttt tggttctggt ttactggcta tcggtgcttc ttcgctagtt
      841 ttacttggca tttcctatgt agctcgcttc ttcggtctac tcattgttcg tctggtacaa
      901 tttgttctta aaaaaggaaa gagaggtaat cagcatgcgt aaatggacaa aaggatttct
      961 catctttggt gtggtgacta ccgttatcgg ctttatcctg ctttttgtag gtatccaatc
     1021 tgacgggatt aagagcctac tttccatgtc caaagaacct gtctatgata gccgtacgga
     1081 aaagctaacc tttggcaagg aagtcgaaaa cctagaaatt actctccacc aacacacgct
     1141 caccatcaca gactctttcg atgatcaaat ccacatttct taccatccat ctctttctgc
     1201 tcaccatgat tttatcacca atcagaacga tagaactctg agtctcactg ataagaaact
     1261 gtctgaaact ccgtttctct cttctggaat tggtgggatt cttcatatcg caagtagcta
     1321 ctctagtcgt tttgaagaag ttattctccg actaccaaaa gggagaactc taaaagggat
     1381 caacatctca gccaatcgcg gacaaaccac catcataaat gctagccttg aaaatgcgac
     1441 cctcaataca aacagctata tcctccgaat tgaaggaagt cgtatcaaaa acagtaaact
     1501 cacaacgccc aatatcgtta atatctttga tacagttctt acagatagtc agctagagtc
     1561 aacagataat cacttccacg ctgaaaatat ccaagtccat ggtaaggttg aactgactgc
     1621 caaagattat ctcagaatca tcctagacca gaaagaaagc caacgaatta actgggacat
     1681 ctcaagtaac tacggttcta tcttccaatt cacaagagaa aagcctgaat caagaggtac
     1741 ggaattaagc aacccttaca aaactgaaaa aaccgatgtc aaggatcaac tcattgcgag
     1801 atctgatgat aatattgatc taatatccac accaagcaga cgttgacaaa aacgcttaca
     1861 tctgctacta tggaagcata tttatctaac caaaaggagg ttctaccatg aaacaagaat
     1921 ggtttgaaag taatgatttt gtaaaaacaa caagcaagaa caagcctgaa gagcaagctc
     1981 aagaggttgc agacaaggct gaagaaacga tagccgattt cgatacacca attggaaaaa
     2041 atactcaagt agaggaggaa gtctctcaag ctgaagtcga actggaaagc cagcaagaag
     2101 agaaaattga aactcctgaa gtcggtgaag cgagaacaga aatagaagaa aagaaggcat
     2161 ctaattctac tgaagaagag tcagaccttt ctaaagaaac agaaaaagtc actatagctg
     2221 aagagagcca agaagctctt cctcagcaaa aagcaaccac gaaagagccg cttcttatca
     2281 gtaaatcttt agaaagtcct tatatccccg accaagctcc aaaatctagg gataaatgga
     2341 aagagcaagt gcttgatttt tggtcttggc tagtggaagc gatcaaatct cctacaagta
     2401 agtttgaaac aagtatcaca cacagttaca cagcctttct cttgctcatt ctgttttctg
     2461 catcttcctt tttctttagt atctatcaca tcaaacatgc ttactatgga catatagcaa
     2521 gcattaacag tcgcttccct gagcagctag ctcctttaac tcttttttct atcatctcta
     2581 tcctagtagc gacaacactc ttcttctttt cattcctctt gggtagtttc gttgtgagac
     2641 gatttatcca ccaggaaaag gactggacac tagacaaggt tctccaacaa tatagtcaac
     2701 tcttggcaat tccaatcttc ctcactgcta ttgctagttt ctttgctttc tttgatagcc
     2761 tacgatttac agccctcttg tgtgtgatta gcattggaat cattctgctt gccagtctcc
     2821 atatcattac aagaccaagt caagcaagtg aaaccgactc cttctatcaa ttattcttgt
     2881 ctgtccttgt gaacggagtt attatcctcc tcttctttgt agctgaagtg gcactgattg
     2941 gagattatct tcgtatcttg gcctttcttt aaacttcata ctcttcgaaa atcaaattca
     3001 aaccacgtca gcttcgcctt accgtactca agtacagcct gcggctagct tcctagtttg
     3061 ctctttgatt ttcattgagt agcaatcctg tcatccttgg caggattttt tctatgtcaa
     3121 aaaagcaagt cgattgacct gcttctgctt tacttttctt gtgctaatgt tttaaaatct
     3181 tgtcctagga tgggttgtat ttctatttga ttggcgtcta gttggtggta aaaagatggg
     3241 ggtaatacct ctaagaattt tgcatagtcc aagcgagcta tggattcata gtcttctggt
     3301 ttagagccgt ctcctgtgat ttgaaccaag taaatctccc actggacttc cttatccacc
     3361 aattgctcaa tataggctgc aggaacaaac ggttctctcg gtaaaactgt cttgactcct
     3421 tgagctaaat ggtaaacgcc atgcactttt ccttctccat cctgatagaa ggaaactctt
     3481 gcaaagtagg catctgtctc ttgaaccgca cgcacacgcg cttcaaactg agccaagcca
     3541 tctgcctcta aaaagctttt caagttctca cgactaaaga aaataggatt aaaaattcct
     3601 tggcaaatcg taaaacgagc tacagggtct gctagatagg cttgaatact tgcttggaaa
     3661 taagcttcaa aatcaaaacc atctagccct tcaatatcag tccctgtata agcaagcaaa
     3721 gcatctaaaa atgacttgat aatctgccag tctgtctttg ttagtaggtc aggaagatcg
     3781 acacggtaag ccttttgacc atcagcataa ctgaccttaa agagaaattg tgattgcccc
     3841 actatcgcac actcaatata ctcgagacga ttaagaggct gacgaagata gactacatca
     3901 tagctatgtg actccaaacc gtctagcaag gccaagatcg acttggcatt caaaatctct
     3961 tgttctccta aaatgctctg tttatttgga ataaaaaatg tttttgccat aactggactc
     4021 ctttgaaatc gtttacatac agtatacctt attttcctga aagatacaaa aacaaacttt
     4081 agatttttga gtacaaagca tagaaaaaca gcccctgagg actgtttgat cttatacagt
     4141 agctgcctga tccaagcttt caccgatagc ggctaggcgc tcaatcactt cagcttgtgt
     4201 caattcattt tttgaaacat agcggttacg tgggtgaaca cggcactcgt gtgagcatcc
     4261 acgaaggtac ttgtcttcat tttcttctga tgtcaagata cgacggttac agaagggatt
     4321 tccacagttg acataacgtt cacatggtgt tccatcaaac cagtctttcc ctacgatggt
     4381 tgggttgaca tggttgacat caacggcaat acgctcgtca aagacgtaca ttttcccatc
     4441 ccaaagctca ccttgaactt ctgggtcttt accgtaagtt gcgattcctc cgtgcaattg
     4501 gccgacatct ttgtagcctt cacggaccat ccagcctgag aatttctcac agcgaacgcc
     4561 acctgtacag taaaccacga cacgcttgtc catgaatttt tccttgttat cacggaccca
     4621 ttgtggtaac tcacggaagt tgcgaatatc tgggcgaata gccccacgga aatgtcctag
     4681 gtcgtactca taatcgttac gtgtgtcaag gacaacggta tctttatcaa gaagcgcttc
     4741 tttgaactct tttggagaca agtaagcacc tgttgtttca agtgggttga tgtcattgtc
     4801 aaagtcgttg tcttccaaac caaggtggac aatttctttc ttgtagcgaa caaacatctt
     4861 cttgaaggct tgttcatttt cttcgtcaat cttgaaccag agatcttcca tacctgggag
     4921 gctgtgaacg tagtccatgt atttttgagt tgtttcatag tcacctgaaa ctgttccgtt
     4981 aattccctcg tcagcgacta ggatacggcc tttaaggccg attgatttac agaaagccaa
     5041 gtggtctgca gcaaattgct ctgcattttc aattggagta taaaggtagt aaagtaagac
     5101 acgaatatct tttgccataa gatttgt
//
LOCUS       DAVMEI010000117         5001 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVMEI010000117 DAVMEI010000000
VERSION     DAVMEI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.117"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..888
                     /locus_tag="VYQ24_001587"
     CDS             <1..888
                     /locus_tag="VYQ24_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1968707.1"
                     /translation="LGKAPQTIHTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRK
                     RSVKKSSLTKELKEKILHYHNQKFSPEMMVIAKGVNVGISTIYYWIHRGKLGLSKQDL
                     LYPRKGKALKKQASTNFKPAGQSIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVL
                     TDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIY
                     YAHPYASWERGTNENHNRLIHRWLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPRE
                     DFLMANLNLKFSVRNKSRN"
     gene            914..1558
                     /locus_tag="VYQ24_001588"
     CDS             914..1558
                     /locus_tag="VYQ24_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1968708.1"
                     /translation="MLRAGVLDANNGIISIAGVVIGVASTTTNIWIIFLSGFAAILAG
                     AFSMAGREYVSVSTPKDTEEAAVLREKLLLDQDRELAKKSLYAAYIQNGECKTSAQLL
                     ANKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSEYR
                     IPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTRGVTFLLEQLFSI"
     gene            complement(1600..3588)
                     /locus_tag="VYQ24_001589"
     CDS             complement(1600..3588)
                     /locus_tag="VYQ24_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968709.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3590..4348)
                     /locus_tag="VYQ24_001590"
     CDS             complement(3590..4348)
                     /locus_tag="VYQ24_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968710.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPTRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSATLLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQLFQEISDTLTVMASEVN"
     gene            4776..4967
                     /locus_tag="VYQ24_001591"
     CDS             4776..4967
                     /locus_tag="VYQ24_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968711.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
BASE COUNT         1541 a          995 c          993 g         1472 t
ORIGIN      
        1 cttggaaaag ctcctcaaac tatccacact gaaatcaagc gtgggacagt ccgacaatgt
       61 cttggaaaag ggcgcttcaa agaggtttat tctgccgact atgctcaaca gtcttatgaa
      121 aacaatcgca agcgctcggt caagaaatca agcttgacca aggaactaaa ggaaaagatt
      181 ctccactatc ataaccaaaa attttcgcct gaaatgatgg ttattgctaa aggggttaac
      241 gtgggaattt caaccattta ctattggatt catcgtggaa aattggggtt aagcaagcag
      301 gatctgcttt accctagaaa aggaaaagcg cttaagaaac aggctagcac caactttaaa
      361 cctgctggtc aatccatcga acagcggcct gaagctatca atcttcgctt ggagaatggg
      421 cattatgaga ttgatacggt tctacttacg agagcgaaaa actactgctt gcttgtcttg
      481 acggatcgaa agagtagaca tcagatcatc cgattgattc caaataaaag tgctgaggtg
      541 gtcaatcagg ctctaaaact catcttaaaa caacacaaga ttctttccat cacggcagat
      601 aatggaacgg aattcaatcg cttgtttgat gtattttctg aggagcacat ctattatgcg
      661 cacccctatg cctcttggga aaggggaact aatgagaatc acaacaggct cattcataga
      721 tggttaccta agggaaccaa gaaaatgact cccaaagaag tcgcattcat cgaaaagtgg
      781 attaacaact atcctaaaaa atgcttgaac tacaagtcac ccagagaaga cttcttgatg
      841 gctaacttga acttgaaatt tagcgtcaga aacaaatcac gaaattgatt caaattttgc
      901 aggtcgttta aatatcctgc gtgcgggtgt tcttgatgct aacaatggaa ttatttccat
      961 tgctggtgtg gttatcggag ttgccagtac cacgaccaat atctggatta tctttttatc
     1021 aggttttgcg gctatcttag ctggtgcctt ttcaatggct ggtagagaat atgtatccgt
     1081 ttcaactcca aaagataccg aggaagctgc cgttttgcga gaaaaactct tgctagacca
     1141 agatagggaa ctagccaaaa aatccctcta tgctgcttat atccaaaatg gagaatgcaa
     1201 aacttctgcc caactcttgg ctaataagat ctttcttaaa aatccactca aggctctggt
     1261 agaggaaaaa tatgggattg agtatgaaga atttaccaat ccttggcacg ctgccatttc
     1321 tagcttcgtt gcctttttcc ttagaagttt gcctccaatg ctgtcagtga ccatattccc
     1381 aagtgaatat cgcatccctg ctactgtcct tattgtcggt gtggcccttc ttctcactgg
     1441 ttacactagt gccagacttg gaaaggatcc gactagaaca gctatgattc ggaaccttgc
     1501 tattggtctc ttgaccaggg gagttacctt cctgctcgaa caacttttca gcatttagaa
     1561 tacaagaaat acctcgattt tgaagtcgag gtatcttttt tacatttgca caatcttgcg
     1621 ataacttctt gaagtaatca taaaaatcag cacataggcg atgaggaaga tagcgcagat
     1681 agacaaggtc acaatcaaca tcatagtcgt atccagtaca ccaatcactt ttaaaatcag
     1741 gctaagcata tggtaggcaa aggcgagatg tatgaaggca aagagcaaag gaaggaagaa
     1801 aacagttaaa acctgtttgt tgatggtttg cttgatttgc ttttggtcca aaccgacttt
     1861 ctgcaagata ataaagcgtt cacggtcttc gtagccttca gaaatttgtt tgtagtagat
     1921 gaccagaaca gttccgacca taaagataat ggataggaaa ataccgataa agaagacacc
     1981 accaaagagg gcactcatct gagaactagc atctgctaga ttgctaccat aaacatagct
     2041 accttctgtg tctaattgag cattaaattg attgaggtag ttttcatact cctcagcgac
     2101 cttgagttgt tcttcttcgc tggcatctac attcatacca ccgtaaaact gattatagat
     2161 agccgaatct gggaattgat ccaaaaaggc ttgtaaatca ggaacaacaa ggtaattgta
     2221 atcagtagtc aagatattaa acttatttgg aacatggttg acaatgaaat ctttattaaa
     2281 ttcttctttg acagaaaatt gatgatcatt tagagttaga gctttctgtc ctttcagtcc
     2341 gtcatttttg gcaaagagac cgacctcatt tcctgataga gacagttttt gaccagtcat
     2401 attttcataa tctttttggt caaataccat gaaaactgtt tttggttgga cacggttttg
     2461 tcctttttca aaaatagtta acttggttcc ttcttgattt gcaataccaa agttactgta
     2521 acgaagcact tctttctctt taatcttata acctttgtca cttgcaaact ggctcaagag
     2581 tttgtccaaa tcttcttttt caacattttg ccctgaaacc ccaaaatcat gaggatttag
     2641 aactttttta aaggattctg cggaattgaa aatgcttgtc gctgctgaca tggttaccaa
     2701 aaccattgtt gacaaaatag cgatagttgc tagtccaact gcatttttct tcatacggaa
     2761 aatcaagtta gaaacagata tgagattatt aggttggtaa tagtatttct tatttttctt
     2821 taagatttga aggaaaacgg taatccctgc attgaacaaa agataagtcc caaagataac
     2881 cagtaaaaca gctatgaaga aagttgttaa ggctgtaaga ggatctttta ccgtaagggc
     2941 aagataatat ccaatcccta aacttatgga accaagaata gtttggagag gaaggaagcg
     3001 accttttttc tctccgctag ctttctcacg tgagagctgg agggcattca tacgggcgat
     3061 tcgaagggca ttcaggaaca tgaggcctag gaaaatcaaa ccgaagacaa gaagtactgt
     3121 aatgacaact ttcatctgga aggtagcgac cagttccacc ttcaatttca tcagtttgag
     3181 taggaaagcg aaaatcaact tgtcaaacaa ggctccaata ccgattcctg ctccaacagt
     3241 tagaatccca aatagcacta actccttaaa ggtcatactg attagatggc gcttctccaa
     3301 gcctaacatg ccatatatac ccagttcctt ggaacggttt ttcatgacaa aactattggc
     3361 atagaggacg ataatggctg acgcaagggt aacgacaaac ataccaaatc caagtgttgc
     3421 ttgaatggtg gttcctccac ggatttccgc aatcttggga ttgaaggtta gggagtaaaa
     3481 gagataggtg atggtgactg ccaagagaac agccagtgca aagggatagt agagtttgcg
     3541 gtttttaatc aagttcgata ccgctaactt attggttaat cgaaacatac taattcacct
     3601 cgcttgccat gacagtcaag gtatcagaga tttcttggaa caactgacgc tctgtcttct
     3661 ctccacggta gatttggttg taaagaatgc cgtctttgat aaagagaaca cgcttggccc
     3721 tgctagcagc tgctgttgag tgggttacca tgaggatggt ttgcccacgc tcattgattt
     3781 cattaaagac atcaagtaag gttgcagatg acttggaatc aagggctcct gttggctcgt
     3841 ccgcaaggag aatttcaggt tctgtgatga tggcgcgggc tactgctaca cgctgtttct
     3901 ggccaccaga aatctcgtaa gggtacttct cttgcaattg gttaataccc agattctcag
     3961 ctgtcaccac caatttcttc atcatctccg ttataggtct tcttgacaag acaagcggaa
     4021 gcaagatatt gtccttaaca gacagagtat ctagcaagtt aaagtcttgg aagacaaatc
     4081 ctagcttttc acgccggaaa ctagaagcct gtgaattttt aatagttgcg gtgtcagttc
     4141 cattcaagta aacctgccca cgagttggtt tatccaacat agctagaata ttgagaagag
     4201 ttgatttacc agaaccagac tcacccatga tggcaacgta gtcacccttt tctacggtaa
     4261 agtgaatatc cttgagggct tctacttggt tgccctgaaa acgtgtttta taaatttttt
     4321 gaacgtgttt tacatctaaa agtgtcatga gaatctcctt tcttaatatc ccatcaaatg
     4381 aaatgtagct tgcatcatag tgcatcccag ctgaacgcgc tcaatttctt tgtgacttgg
     4441 ttttctagtt ttcttatgta agatttgttt catgatgtta ctcctttgtt ctttatgagt
     4501 ctagtttaca tcaaaaaaag accgcttgcc ataacctaac cttacaaaag agactttaat
     4561 cttacatttt tgtaagattg ggtgaaatat aagcaaaatt acttagaaaa tgatttgatt
     4621 tcctattttt aaagttgtaa aatataagtg tgaaaaggct tactttgaaa aggaggaagc
     4681 gggaactaac attttagtaa gcttacctag ataacatacc attcaattta acacttgcaa
     4741 gtcgttcaac ctataaggaa agaaggtgcg ctttaatgaa acacaaagaa catatactca
     4801 tcggacttct ctatctgctc tctccattca tcggtcagct cttagttgaa cacacacact
     4861 ttatcagtac agaatttacc ggcactgctt atgttatttg ctggttatca gttgttatca
     4921 gcatccacca tttttccaaa aatgtattgt ctcaacagca aaaatagatt aaaaaactgc
     4981 gtttaaatta ctgcttttat a
//
LOCUS       DAVMEI010000118         4958 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVMEI010000118 DAVMEI010000000
VERSION     DAVMEI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.118"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            220..483
                     /locus_tag="VYQ24_001592"
     CDS             220..483
                     /locus_tag="VYQ24_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1968712.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            489..2222
                     /gene="ptsP"
                     /locus_tag="VYQ24_001593"
     CDS             489..2222
                     /gene="ptsP"
                     /locus_tag="VYQ24_001593"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1968713.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2477..4927
                     /gene="phtA"
                     /locus_tag="VYQ24_001594"
     CDS             2477..4927
                     /gene="phtA"
                     /locus_tag="VYQ24_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1968714.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
BASE COUNT         1675 a          936 c         1046 g         1301 t
ORIGIN      
        1 ttgtgaaata gcaatgatta tcctataaat tgtttttgaa gaaactatat cctgtgtttt
       61 gttatctagg atagaagatt ttcagcaagt tatctgaaag gaaattgaag gaaaatccag
      121 aaaatgcttg aaaaaaatcc tagaagatgg tataatacta aattgtaagg gttatcacat
      181 ataactcaaa aaaagaaaga acaaaaggag agtcaaacta tggcttctaa agatttccac
      241 gtagtggcag aaacaggtat tcacgcacgt ccagcaacat tgttggtaca aactgctagc
      301 aaatttgctt cagatatcac tcttgagtac aaaggtaaat cagttaacct taaatcaatt
      361 atgggtgtta tgagtcttgg tgttggccaa ggtgctgacg taactatctc agctgaaggt
      421 gcagatgcag atgacgctat cgctgcaatc tcagaaacaa tggaaaaaga aggattggca
      481 taagggaaat gacagaaatg cttaaaggaa tcgcagcatc tgacggtgtt gcagttgcaa
      541 aagcatatct actcgttcag ccggatttgt catttgagac tattacagtc gaagatacaa
      601 acgcagaaga agctcgcctt gatgccgctc tacaggcatc acaagacgag ctttctgtta
      661 ttcgcgagaa agcagtaggt acgctcggtg aagaagcagc tcaagttttt gatgctcact
      721 taatggttct tgctgaccca gaaatgatca gccaaatcaa ggaaactatc cgtgcgaaga
      781 aagtgaatgc agaagcaggt ctgaaagaag ttacagatat gtttatcact atctttgaag
      841 gcatggaaga caacccatac atgcaagaac gcgcagcgga tatccgcgac gtgacaaaac
      901 gtgtattggc aaaccttctt ggtaaaaaat tgccaaaccc agcttctatc aatgaagaag
      961 tgattgtgat tgcgcatgac ttgacaccat ctgatacagc tcaattggac aaaaactttg
     1021 taaaagcttt tgtaaccaac attggtggac gtacaagcca ctcagctatc atggcacgta
     1081 cacttgaaat tgctgctgta ttaggtacaa ataacatcac tgaaatcgtt aaagacggtg
     1141 acatccttgc tgttaacggg atcactggag aagtgattat caacccaaca gatgaacaag
     1201 cggcagaatt taaagcagct ggtgaagcct atgcgaaaca aaaagctgaa tgggcacttt
     1261 tgaaagatgc tcaaacagtg actgctgacg gtaaacactt cgagttggct gccaatattg
     1321 gtactccaaa agacgttgaa ggtgttaaca acaacggtgc agaagctgtt ggactttacc
     1381 gtacagagtt cttgtacatg gattctcaag acttcccaac tgaagatgag cagtatgaag
     1441 catacaaggc tgttcttgaa ggaatgaacg gtaaacctgt tgtcgttcgt acaatggata
     1501 tcggtggaga taaggaactt ccttacttcg atatgcctca cgaaatgaac ccattccttg
     1561 gattccgtgc tcttcgtatc tctatctctg agactggaga tgctatgttc cgcacacaaa
     1621 tccgtgctct tcttcgtgcg tctgttcacg gtcaattgcg tatcatgttc ccaatggttg
     1681 cgctcttgaa agaattccgt gcagcgaaag cagtctttga tgaagaaaaa gcaaaccttc
     1741 ttgctgaagg tgttgcagtt gcggataaca tccaagttgg tatcatgatc gagattcctg
     1801 cagcggctat gcttgcagac caatttgcta aagaagttga cttcttctca attggtacaa
     1861 acgacttgat ccaatataca atggcagcag accgtatgaa cgaacaagtt tcataccttt
     1921 accaaccata caacccatca atcctacgct tgattaacaa tgtgatcaaa gcagctcacg
     1981 ctgaaggtaa atgggctggt atgtgtggtg agatggctgg tgaccaacaa gctgttccac
     2041 ttcttgtcgg aatgggcttg gatgagttct ctatgtcagc aacatctgta cttcgtacac
     2101 gcagcttgat gaagaaactc gacacagcta agatggaaga gtacgcaaac cgtgccctta
     2161 cagaatgctc aacaatggaa gaagttcttg aacttcaaaa agaatacgtt aattttgatt
     2221 aatcgaaaag tccctgcaac tcagttacag ggactttttt tgatatttta aaaagaattt
     2281 tcaagaaaat ctttcttata aaaagtccaa ccttgaaaaa gtagtggtca gaacaaaaaa
     2341 tacttaaatg gttcataaaa ttcttgacaa gttggagatt taggagtaaa ctattaacca
     2401 gttaagtaat agagaggagt ttctgcaatt tagaaatgaa ttgcaactag aaatatcaaa
     2461 tagaaagaga gtttcgatga aaattaataa gaaatacctt gttggttctg cggcagcttt
     2521 gattttaagt gtttgttctt acgagttggg actgtatcaa gctagaacgg ttaaggaaaa
     2581 taatcgtgtt tcctatatag atggaaaaca agcgacgcaa aaaacggaga atttgactcc
     2641 tgatgaggtt agcaagcgtg aaggaatcaa tgctgagcaa atcgtcatca agataacaga
     2701 ccaaggctat gtcacttcac atggcgacca ctatcattat tacaatggta aggttcctta
     2761 tgatgcgatt ttcagtgaag aattactcat gaaagatcca aactataagc taaaagatga
     2821 ggatattgtt aatgaggtca agggtggata tgttatcaag gtagatggaa aatactatgt
     2881 ttaccttaag gatgctgccc acgcggataa cgtccgtaca aaagaggaaa tcaatcgaca
     2941 aaaacaagaa catagtcaac atcgtgaagg tgggactcca agaaacgatg gtgctgttgc
     3001 cttggcacgt tcgcaaggac gctatactac agatgatggt tatatcttta atgcttctga
     3061 tatcatagag gatactggtg atgcttatat cgttcctcat ggagatcatt accattacat
     3121 tcctaagaat gagttatcag ctagcgagtt ggctgctgca gaagccttcc tatctggtcg
     3181 gggaaatctg tcaaattcaa gaacctatcg ccgacaaaat agcgataaca cttcaagaac
     3241 aaactgggta ccttctgtaa gcaatccagg aactacaaat actaacacaa gcaacaacag
     3301 caacactaac agtcaagcaa gtcaaagtaa tgacattgat agtctcttga aacagctcta
     3361 caaactgcct ttgagtcaac gacatgtaga atctgatggc cttgttttcg acccagcgca
     3421 aatcacaagt cgaacagcta gaggtgttgc agtgccacac ggagatcatt accacttcat
     3481 cccttactct caaatgtctg aattggaaga acgaatcgct cgtattattc cccttcgtta
     3541 tcgttcaaac cattgggtac cagattcaag gccagaacaa ccaagtccac aaccgactcc
     3601 ggaacctagt ccaggcccgc aacctgcacc aaatcttaaa atagactcaa attcttcttt
     3661 ggttagtcag ctggtacgaa aagttgggga aggatatgta ttcgaagaaa agggcatctc
     3721 tcgttatgtc tttgcgaaag atttaccatc tgaaactgtt aaaaatcttg aaagtaagtt
     3781 atcaaaacaa gagagtgttt cacacacttt aactgctaaa aaagaaaatg ttgctcctcg
     3841 tgaccaagaa ttttatgata aagcatataa tctgttaact gaggctcata aagccttgtt
     3901 tgaaaataag ggtcgtaatt ctgatttcca agccttagac aaattattag aacgcttgaa
     3961 tgatgaatcg actaataaag aaaaattggt agatgattta ttggcattcc tagcaccaat
     4021 tacccatcca gagcgacttg gcaagccaaa ttctcaaatt gagtatactg aagacgaagt
     4081 tcgtattgct caattagctg ataagtatac aacgtcagat ggttacattt ttgatgaaca
     4141 tgatataatc agtgatgaag gagatgcata tgtaacgcct catatgggcc atagtcactg
     4201 gattggaaaa gatagccttt ctgataagga aaaagttgca gctcaagcct atactaaaga
     4261 aaaaggtatc ctacctccat ctccagacgc agatgttaaa gcaaatccaa ctggagatag
     4321 tgcagcagct atttacaatc gtgtgaaagg ggaaaaacga attccactcg ttcgacttcc
     4381 atatatggtt gagcatacag ttgaggttaa aaacggtaat ttgattattc ctcataagga
     4441 tcattaccat aatattaaat ttgcttggtt tgatgatcac acatacaaag ctccaaatgg
     4501 ctataccttg gaagatttgt ttgcgacgat taagtactac gtagaacacc ctgacgaacg
     4561 tccacattct aatgatggat ggggcaatgc cagtgagcat gtgttaggca agaaagacca
     4621 cagtgaagat ccaaataaga acttcaaagc ggatgaagag ccagtagagg aaacacctgc
     4681 tgagccagaa gtccctcaag tagagactga aaaagtagaa gcccaactca aagaagcaga
     4741 agttttgctt gcaaaagtaa cggattctag tctgaaagcc aatgcaacag aaactctagc
     4801 tggtttacga aataatttga ctcttcaaat tatggataac aatagtatca tggcagaagc
     4861 agaaaaatta cttgcgttgt taaaaggaag taatccttca tctgtaagta aggaaaaaat
     4921 aaactaatga aaaatgaaag tctcgataaa gaggcttt
//
LOCUS       DAVMEI010000119         4932 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVMEI010000119 DAVMEI010000000
VERSION     DAVMEI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.119"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            172..597
                     /gene="lacA"
                     /locus_tag="VYQ24_001595"
     CDS             172..597
                     /gene="lacA"
                     /locus_tag="VYQ24_001595"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1968715.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            628..1143
                     /gene="lacB"
                     /locus_tag="VYQ24_001596"
     CDS             628..1143
                     /gene="lacB"
                     /locus_tag="VYQ24_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1968716.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQIDANFFTEF
                     LEKWDRGEYHD"
     gene            1154..2083
                     /locus_tag="VYQ24_001597"
     CDS             1154..2083
                     /locus_tag="VYQ24_001597"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1968717.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            2085..3065
                     /gene="lacD"
                     /locus_tag="VYQ24_001598"
     CDS             2085..3065
                     /gene="lacD"
                     /locus_tag="VYQ24_001598"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1968718.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3299..4135
                     /locus_tag="VYQ24_001599"
     CDS             3299..4135
                     /locus_tag="VYQ24_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1968719.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYCQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4171..4488
                     /locus_tag="VYQ24_001600"
     CDS             4171..4488
                     /locus_tag="VYQ24_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1968720.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            4490..>4932
                     /locus_tag="VYQ24_001601"
     CDS             4490..>4932
                     /locus_tag="VYQ24_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1968721.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAF"
BASE COUNT         1616 a          823 c         1037 g         1456 t
ORIGIN      
        1 atagaagttt tataaaaaag taaaaaataa ataacaaaaa caaacacaaa ctgttgactt
       61 cttcaaacaa tagtagtata ttatgtttgt gaggactaat tgactgtttt ttaaagacag
      121 ccaatacact gctggaatcc agcatagaaa aaataaaaag gagtatacaa tatgtctatt
      181 gttatcggtg cagatgctgc aggtttgaga ttgaaagaag ttgtgaaaga cttcttagaa
      241 aaagaaaact tccaccttgt ggatgttaca gctgaaggtc aagactttgt tgatgtgact
      301 ctcgctgttg ctgcagaagt aaacaaagaa gaacaaaacc ttggtatcgt gattgatgct
      361 tatggagctg gtccatttat ggttgcaact aagatcaaag gaatggttgc tgcagaagtt
      421 tctgatgaac gttcagctta tatgactcgt ggccacaaca actcacgtat gatcactatg
      481 ggtgcacaac ttgttggtga tgaattggct aaaaacatcg ctaaaggatt tgttaatggt
      541 aaatacgacg gtggtcgtca ccaaatccgg gttgacatgt tgaacaaaat gggctaatta
      601 gattatagta agaaaggtaa gttaaaaatg agaattgcaa ttggatgtga ccacatcgta
      661 acagatgaaa aaatggcggt ttcagaattt ttgaaatcaa aaggatatga agtcattgac
      721 tttggtacat atgaccatac acgtactcac tacccaatct ttggtaaaaa agtaggggaa
      781 gctgtaacta gcggtcaagc tgatcttgga gtatgtatct gtggtactgg tgttggtatc
      841 aacaacgctg taaataaagt tccaggtgtt cgttctgcct tggttcgtga tatgacaaca
      901 gccctttatg ctaaagaaca attgaacgct aacgttattg gttttggtgg taagattact
      961 ggtgaattgc ttatgtgtga tatcatcgaa gctttcatcc atgctgaata caaaccaact
     1021 gaagaaaaca aaaaattgat tgcgaaaatt gaacatgttg aaagtcacaa tgctcaacaa
     1081 atagacgcaa acttcttcac tgaattcctt gaaaaatggg atcgcggaga ataccacgac
     1141 taagaggtaa cctatgattt taacagttac aatgaaccca tccattgata tttcctatcc
     1201 cttggatgag ttgaagattg atactgtcaa tcgtgtggtg gatgtaacca aaacggctgg
     1261 tggtaaggga ctcaatgtta cccgagtact ttcagaattt ggcgattctg ttcttgctac
     1321 tggtttagtg ggtggcaaac ttggtgagtt tttggttgaa catatcgata atcaagtaaa
     1381 gaaagatttc ttctcaattc agggagaaac tcgtaactgt atcgctattc tccacggaga
     1441 caaccaaaca gaagttcttg aaaaaggtcc tgaagtattg gaacaggaag gtcaagactt
     1501 tttggaacat ttcaaaaaac tcttggagtc agttgaagta gtggccatct caggtagtct
     1561 gccagctggc cttccagttg attactatgc gagcttggta gaacttgcta atcaagctgg
     1621 caagcctgta gtcttggact gctcaggtgc agcacttcag gctgttcttg aatcacccca
     1681 taaaccaaca gtcatcaaac caaataatga agaattgtct cagcttcttg gaagagaagt
     1741 ttctgaggat ttggatgaat taaaagaagt acttcaagaa cctttgtttg cagggattga
     1801 atggattatc gtttcacttg gtgccaacgg tacttttgcc aaacatggtg acactttcta
     1861 caaggtagat attcctagaa ttcaggtggt aaatcctgtt ggatctggag actctactgt
     1921 ggcaggaatt tcttcaggac ttcttcacaa agaatcggat gcagaattac tcatcaaggc
     1981 aaatgtcctt ggtatgctca atgctcaaga aaaaatgact ggtcatgtca acatggccaa
     2041 ctatcaagtt ctatatgatc aattaatagt aaaagaggta taaaatggct ttaacagaac
     2101 aaaaacgtgt acgcttagaa aaactttctg atgaaaatgg tatcatctca gctcttgcat
     2161 ttgaccaacg tggtgctttg aaacgcctca tggctcaaca ccaaacagaa gaaccaactg
     2221 tggcccaaat ggaagaactt aaagtcttgg tagcagatga attgactaaa tacgcttcat
     2281 ctatgcttct tgaccctgag tatggacttc cagcaactaa agctcttgat gaaaaagctg
     2341 gtcttctcct tgcttatgaa aaaacaggtt atgacacaac aagcacaaaa cgcttgccag
     2401 actgcttgga tgtctggtct gcaaaacgta ttaaagaaga aggtgcagat gcagttaaat
     2461 tcttgcttta ctatgatgta gatagctcag acgaactcaa tcaagaaaaa caagcctaca
     2521 tcgaacgcat cggttctgag tgtgtggctg aagatatccc attcttcctt gaaattcttg
     2581 cttacgatga aaaaattgcg gatgcaggtt ctgtagaata cgcgaaagta aaaccacaca
     2641 aagttatcgg cgctatgaaa gtcttttcag acccacgctt taacattgat gtcttgaaag
     2701 ttgaagttcc tgttaacatt aaatatgttg aaggcttcgc tgaaggtgaa gtagtttata
     2761 cacgtgaaga agcagcagcc ttcttcaaag cgcaagatga agcaacgaac ttgccataca
     2821 tctacttgag tgctggtgta tcagctaaac tcttccaaga tactcttgta tttgctcatg
     2881 aatcaggtgc aaactttaac ggagttcttt gtggccgtgc aacatgggct ggatcagttg
     2941 aagcatacat caaagatggt gaaacagcag ctcgcgaatg gcttcgcaca actggatttg
     3001 aaaacattga tgagctcaac aaagttcttc aaacaacagc aacttcatgg aaagaacgtg
     3061 tgtaagaaag tcctcctagt ttaggaacat gaatctaaaa aaatttaaaa aaagttgtat
     3121 gtaaaggttt acaaaataac ttacttgtgc tatacttaaa tcacaagtta atacaaggtg
     3181 agtgttacta agtaatatta ggcatgatca caggtgaatt agaaatcagc tgattttcta
     3241 gttcatttgt ggtcattttt tgtactcata tacctttaag atataaaagg aggttgacat
     3301 gtatcgaatt ctaaatccaa tgaatcacaa tgtctcgctt gtcagaaatg ataagggaga
     3361 agaggtgatt gtaattggta aggggattgc attcggaaag aagaaggggg atttgattgc
     3421 tgaaaatcag gttgagaaaa tctttcggat gaagaccgaa gagtccagag aaaactttat
     3481 ggctcttctc aaagatgttc cgcttgattt tatcacagtg acctatgaaa tcattgataa
     3541 gctatcaaag aaatatcatt atccgattca agagtatctc tatgtaacct tgacagatca
     3601 tatttactgt tcttatcaag ctctaactca aggaaggtac aaggatagta atctgccaga
     3661 tatttctgct aagtatcctg tcgcttttca aatcgcaaat gaagcttttg aaatttactg
     3721 tcagaagcta gcagatcatt ttcctgagga cgaaattatt cggattgctt atcatttcat
     3781 taatgctgaa ggtgaaaatg aagtggaact tgtggagtcg attgataaga ggaaagaaat
     3841 tctcaggaat gttgaagaag ttttaacgga ctatgcaatt caacgaacta aaaagaataa
     3901 ccatttctat gatcgcttta tgatccattt gaattatttc ttggattatt tagacagatc
     3961 tagagatgat aaccaatcac ttctggatat ggaagatcat attaaacaat cctatccaaa
     4021 agccttcgag attggttcca agatctatga tgtgattacg caacatacgg gtcttgattt
     4081 gtataaaagt gaacgagttt atctagttct acatatccaa cgtttattgt cataaaaatt
     4141 tatttaaaac tatataagga gaattctatc atgaatagag aagaagtaac attgttaggt
     4201 tttgaaatcg tagcctatgc tggcgatgct cgttcaaaac tattggaagc cttgaaggct
     4261 gctgaagctg gtgattttga aaaagcggac gctctggtag aggaagctgg tagctgtatt
     4321 gcagaggctc accacgcgca aacaagtcta ttgactaagg aagcttctgg tgaggactta
     4381 gcgtatagtg tgaccatgat gcatggtcaa gatcacttga tgacaactat cttgttaaaa
     4441 gacctaatgc accatttaat tgaactttac aagagaggag ttaaataaaa tggataagtt
     4501 aataattttt attgagaaag gaaagccttt ctttgagaaa ttatcgagaa atatatactt
     4561 aagagcgata aaagatggtt ttatttccag tatgccagca gtattattct caagtatttt
     4621 tatactaata gctgctgtgc ccaatatttt tgggtttaaa tggtctgatg aacagttagc
     4681 ttttattttg aaaccttaca actactcaat ggggatttta gctctcttag tagctgggac
     4741 aactgcaaaa tcgttaacag attcagttaa tacacgtagt atggagaaaa ccaaccaaat
     4801 caactacatg tcaacttttt tagcagctgt tgttggattg ttaatactgg cagctgatcc
     4861 aattgaaggt ggatttgcta atggtctttt aggtactaga ggattactta ctgcattttt
     4921 ggcagctttt at
//
LOCUS       DAVMEI010000120         4917 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVMEI010000120 DAVMEI010000000
VERSION     DAVMEI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.120"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..97
                     /locus_tag="VYQ24_001602"
     CDS             <1..97
                     /locus_tag="VYQ24_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1968722.1"
                     /translation="IVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(214..1017)
                     /locus_tag="VYQ24_001603"
     CDS             complement(214..1017)
                     /locus_tag="VYQ24_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1968723.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1294..2049)
                     /locus_tag="VYQ24_001604"
     CDS             complement(1294..2049)
                     /locus_tag="VYQ24_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1968724.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNSRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYVEIVKL
                     LLASEGKQTGITIIEKPIIRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDACLY
                     GDGYDFQVDIQEHSYLAEVKGIRKPKGRVRLTAYEFEKAKEFQSDFILSLVTNLDDIP
                     KLVLVDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2119..4770)
                     /locus_tag="VYQ24_001605"
     CDS             complement(2119..4770)
                     /locus_tag="VYQ24_001605"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1968725.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAINPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPELGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     EKDKQADYQAKYDVTVARIDEMKKLVK"
     gene            complement(4782..>4917)
                     /locus_tag="VYQ24_001606"
     CDS             complement(4782..>4917)
                     /locus_tag="VYQ24_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1968726.1"
                     /translation="ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN
                     "
BASE COUNT         1434 a         1058 c          947 g         1478 t
ORIGIN      
        1 tattgtagac tatattgatt actacaacaa taaacgaatt aaggtaaaac taaaaggact
       61 tagccctgtg cagtacagaa ctaaatcctt cggataaatt aattgtctaa cttttggggg
      121 gcagtacata ttcaacacct gatactatgc gttttattgt gggaatgttt gcttcatttt
      181 ttcctaaaat tcaatgttta ctcagtattt ggattatgaa aaatcgaggt ctaaatctag
      241 atacattttt tctgaagaca aatcattttg accaccgagc aagagatttt caaaaaaagc
      301 tgttaaaaac tcaggacgtc gctgtaaaat ctttgcatta tctaatacca aggcatcacg
      361 aaaatatttg gaatgttgct gaaatggtat attatcaata tcaaaaccaa actcacgaag
      421 atactgaatc aaaaagaccg ttactgtccg agtgtttcct tcgcgaaatg gatgaatctg
      481 ccagattcct gaaataaaat gctggatttg tttaaccaca tccgcctgag ttagtgtcgc
      541 atatgcaact tgtttttcct gattaaaatc ataatctaag gtcatttgaa tcatggagta
      601 atcagagtac acaacacttt caccattcaa aacaggttca ttctttgtga tattggtctg
      661 acgaaattgc cccaccggaa tagagggttc aaatatatct tgaaacaact ccttatgaat
      721 agcaagtaag gtcgcaggac taaagctaaa gcctcttcga gacaatagtt ctacaatacg
      781 tagagaaacc aagtctgcct cttccgtact tgcatcaata gtatggtgat aagccgttgc
      841 atcctcataa acctgctcat aagtcagttc tccccgggac tgtttctcag ccaaagattc
      901 catatacgct gatggcacta gattgtcaac tttctgcaga ccaaaaccta tccgccataa
      961 atcacgcttc gcttcataag acaagtttgg attgtcaatg ttgtaagttg gttgcataga
     1021 aatatccttt caattgtttt tcttcattac cagtaaatca cgaaattggt tggaaactgg
     1081 cttaaactat aaaaagtcct cacaataggt tctatgatat atatatatac ttcatgtaaa
     1141 taataaaaga gttataatca gcactactga taaattcaat accgatttcg ccatcttaaa
     1201 acttagtcaa acgaataccg ttgatttcca gttcattaag tcgttgacct gtcaagactt
     1261 ctcgttcctt caactggaaa agttaagggg gaactaatac aaatcctcta cgcttctata
     1321 ttcaatgatt tcatttttta taatattctt tttaaactca aaatgtttta gaggattatc
     1381 aactaatact aactttggaa tatcatctag gttggtgacc agagataaaa taaaatctga
     1441 ttgaaactct ttagctttct caaattcgta ggcagttaaa cggacacgac ccttaggttt
     1501 tcgaatccct ttaacttcgg caaggtagga atgttcttga atatctactt ggaaatcata
     1561 tccatcacca taaagacatg catcggttaa ctgtccacct tgaaattttt cttctttatc
     1621 aaaatgaagg ataaaataat tctctgcctc catccccgtt tcctgtagac gtttgaattt
     1681 agttcgaata attggttttt caattatagt aatgcctgtt tgctttcctt cactagcaag
     1741 taacagttta acaatttcaa cataactatg aacatcttca tttccaaaca tcatatcaat
     1801 taaatcttta cgaaaacggt aaacttcagc tttctgccac caaccttttc gactattgtc
     1861 aaaataagga tcaaataaat ccattctatt ttttacaact ccagttgttt cagcaattcc
     1921 cagagaaacc acatagcgat aaaactccga cttgctagag cattgaaatt cttttataaa
     1981 aagcttgtca aacttggcaa gaccgtagcc aattaaattt aacaattcat aatgagggtg
     2041 tttggacatc actttctcca tcaatagata ttgatttcat tataccaaaa aacacggctc
     2101 atcaccgtgt ttctgtgttt atttcaccaa cttcttcatc tcatcaatac gtgctacggt
     2161 cacatcatac ttggcttggt agtcggcttg tttgtctttt tctttttgga caacttctgg
     2221 tttagcgttg gcgacgaagc gttcgttgct gagttttttg ccaaccatgt cgagttcttt
     2281 ctgccatttg gcaagttctt tttccagacg tgccaattct tcttcgacat tcaagaggtc
     2341 taccagtggc aagaagattt cggctcctgt aataacgctt gacatagcca gttcaggtgc
     2401 agggatggtt gatgcgattt ccaagtgttc tggatttgta aagcgtttga tatagttgac
     2461 attgctgtta aagaaggctt ccaagtcgct atcgcttgtc ttaacaagga tggtgatagg
     2521 cttgcttggt gctacattta cttccgcacg cgcattccga acagcacgaa tcaagtcttt
     2581 gagactttcc acaccagtgt gagccgcaag gtcttcaaag gctagattaa cagttgggta
     2641 tgcagctgtc acgatagaac cttctgagat ttgtccaaag atttcctctg tcacgaatgg
     2701 catgattggg tgaaggagac gaaggatctt gtccagcgta tagaggagaa cagatcgagt
     2761 aatgacctta tcgtcttcat tgtcgctgta tagaacttcc ttggtcaact caacatacca
     2821 gttggcaaat tcttcccaga tgaagttgta aaggatatga ccagccacac caaactcgaa
     2881 cttatcaaag ttttcagtaa cttttgcaat ggtttcgttg agattgtgga gaatccagcg
     2941 gtccgtcaca ttaccagcct cacctgttgc aacttttgtg acattgtcat gcgccacatc
     3001 cagcgtcaaa ccttcattgt tcatgaggat atagcgagaa atgttccaaa ttttgttaat
     3061 aaagttccat gaagcatcca ttttctcgta agagaaacga acgtcttgac ctggtgcgga
     3121 accgtttgaa aggaaccaac gaagggcatc agcaccgtat ttctcgatga catccattgg
     3181 gtcaatcccg ttaccgagag atttagacat cttgcgtcct tgctcgtcac ggatgagacc
     3241 gtggataagc acgttttgga atggctgacg accagtaaat tccaaggact ggaagatcat
     3301 acgagatacc cagaagaaga tgatgtcgta acctgttacc aaggttgaag ttgggaaata
     3361 acgtttaaag tcttctgagt cgacttcagg ccagcccatg gttgaaaatg gccagagggc
     3421 agaactgaac caagtatcca agacgtcttc gtcctgagtc catccgtcac cttctggagc
     3481 ttcttcgccg acatacattt caccatcagc attgtaccag gcagggattt ggtgacccca
     3541 ccaaagctga cgagagataa cccagtcgtg gacattttcc atccattgaa ggaaggtatc
     3601 gttgaaacga ggtgggtaga attcgacctt gtcctctgtg tcttggttag caatggcgtt
     3661 cttagccaat tggtccatct tgacgaacca ttgagtagac aagcgtggct caactacgac
     3721 acctgtacgt tctgagtgac caacactgtg gacacgtttt tcgattttga caagggcacc
     3781 gatttcttcc aacttagcaa cgactgcctt acgagcttca aaacgatcca tgcctgaaaa
     3841 ttcaaaggca agctcattca tagttccgtc gtcgttcatg acgttgactt gtggcaagtt
     3901 atgacgttgg ccaaccaaga agtcatttgg atcgtgggca ggtgtgattt tcacgacacc
     3961 agtaccaagc tcaggatctg cgtgctcatc tccaacgatt gggatgagtt tattagcgat
     4021 tggaaggatg acgtttttac caatcaagtc cttgtagcgc gggtcttctg gattaatcgc
     4081 aaccgcaacg tccccaaaca tagtctcagg acgagttgta gcaacttcaa gggcgcgtga
     4141 accatcttcc agcatgtaat tcatgtggta gaaggcacct tctacatcct tgtgaatcac
     4201 ctcaatatca gaaagggctg tgcgagctgc tgggtcccag ttgatgataa actcaccacg
     4261 atagatccag cctttcttgt aaaggttcac aaagacctta cgaacagctt ttgacaaacc
     4321 ttcatcaaga gtgaaacgct cacgagaata gtctacagaa agccccatct tgccccattg
     4381 ttccttgatg gtagtggcat attcgtcttt ccattcccag accttcgtca agaaagactc
     4441 acgacctagg tcataacgcg taataccctc accacgtaag cgctcctcaa ccttagcctg
     4501 agtcgcaata ccagcgtggt ccatacctgg aagccaaagg gtatcaaagc cttgcatgcg
     4561 tttttgacgg atgatgatat cctgcaaagt cgtatcccaa gcgtgaccaa ggtgaagttt
     4621 cccagttacg tttggtggtg gaatcacgat tgaataaggc ttagcctttt gatcgcctga
     4681 aggcttgaaa acatccgcat caagccattt ttggtaacga ccagcctcaa cctcggctgg
     4741 attgtattta gatgaaagtt ctttagacat gtgtgttctc cttaattact tttattgaca
     4801 attttaacca aaccaatttt tgaaagttca atataaatct cttcaatttc agcaaatgct
     4861 gcaagtggtg acatagtata atttccagga ttatatttaa gatcatactt gcttatc
//
LOCUS       DAVMEI010000121         4843 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVMEI010000121 DAVMEI010000000
VERSION     DAVMEI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.121"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            400..582
                     /locus_tag="VYQ24_001607"
                     /pseudo
     CDS             400..582
                     /locus_tag="VYQ24_001607"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1194..1478
                     /locus_tag="VYQ24_001608"
     CDS             1194..1478
                     /locus_tag="VYQ24_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968727.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1471..1709
                     /locus_tag="VYQ24_001609"
                     /pseudo
     CDS             1471..1709
                     /locus_tag="VYQ24_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1805..2023
                     /locus_tag="VYQ24_001610"
     CDS             1805..2023
                     /locus_tag="VYQ24_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968728.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2390..4714)
                     /gene="pflB"
                     /locus_tag="VYQ24_001611"
     CDS             complement(2390..4714)
                     /gene="pflB"
                     /locus_tag="VYQ24_001611"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1968729.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT         1552 a          889 c          879 g         1523 t
ORIGIN      
        1 tatatgaaat tctcattttt gtttttacaa ttctccttag ttaaatcttg tttaatatat
       61 gttttacata tagtatttag cgccacatag tactgaactc tctccaaaaa cggttattcc
      121 tctttgaata gggcgttatc acaagaaaag catctccacg tttcaacttc atatggctca
      181 aaaacaatca attgatgcta aaacctgtac ctagatgttt cggttcataa aaccatgaaa
      241 ctgtaaaagt ggatgaaatt gatagcgata gtcaaatcaa gaggcatcat aactctaaaa
      301 agtcacaata tataagttca tcctcggaaa aatatcattc taattgttga aatgcctaca
      361 tgaaaagaaa cgtcaaatgc tcatgaaaca acgaatacag gtatcaaaac tatgacaaaa
      421 caagtcccta aatttactaa agacactgct caactttaca cctgtaaatg gttgttgtat
      481 aataaagtta caaagatgta cgaccacact gttgtaaatc atagtgttcg cgaatatatt
      541 actgatagca tttctacaaa tacaattgaa gagactggat agactttaaa cgaaatatgt
      601 cagtgttttg gcattcctag ccttcatatc atttaaagaa ttctatagac aaaatttttc
      661 ccaatacaga cactcgtaac aactgcttca tttttctacc aacatattta ggaacaggat
      721 aagatacaag aggattaatc catagatcag ttctatacca atctaagaca aataagctaa
      781 aaaaacgatt gataataagc aaatagattc caaattttct ctatctgctc attttaataa
      841 acaatactag tgtaactatc cttccagtca gaagcttgtc aaatcacacc gaaaattctt
      901 ctaaaattta tctcgttagg caatcaagca aaaactcgac gatagtacaa acattatcat
      961 acagaattga cttcctaaat tatatacttt agtaaggttt tcgtataaga aaaaagattc
     1021 attttacatt tctaaacatt cttttctaag atgaaaaaca gaatttttcg attgtgattt
     1081 aaagcaacaa gaagattttc agtatcatcc tatagatacg agctaattaa gaaaaactac
     1141 attttcgaat ataaactaca ataatataaa ctaaatttta taggaggaag acaatggatt
     1201 ggtacgatta tatgatacag gcatccaaac aatcacaatt caacgcaagc cattggtttc
     1261 gctatttgcg aaaagttatt tttgaagact attcttattt aacaaaccaa gatgtagaaa
     1321 agttgctaga ctccaaagaa ctaacccgtt ttcaaaaaat tagcttgaag tatgcctttc
     1381 aagagcatac tccaactcat aaatatgtga tttcattaaa taaacctgct aagttaacca
     1441 atgttcaaaa attgatggag aaatacaaac atggataaaa tgaaaccggt cttccaagcc
     1501 ctaaataaga aattaattca ggaaaatctg actttaacaa ttatggttat gtcttagaat
     1561 atcatggttt acgtgccaca caagatgttg atgcttttta tgaccaaaat cagaaaatca
     1621 atgaaattat tgctcgtgta ggaaaacagt ttaatcttaa catccacgaa gagttatggc
     1681 tcaacaatca tgttgcaaag caaatatgaa caaacaacct cccttaagtt tatgcgaatc
     1741 cttgtactca tttgagaatc tgaccgtttt agtagtccct attgagtatg tgttaggaat
     1801 gaagatgatg agtatcagag agcaggattt gaaagatatt ggtgcaatta ttaaatacaa
     1861 aaactttcat tcaccattcg atacctttaa atatttaaaa gacatgggat ttgatactat
     1921 tgacctctct gttctattag aaggttttag ttatgcttat ggtatggatt ggttagaaaa
     1981 attttttaaa gaaaatcaag ataaacttcg cgaattttat taacagtatc aaacaagatg
     2041 attgtcaatt agccagtcgt cttattttta tgatggaatg aaataaaatc ggaacaaatc
     2101 acttagagaa ttccagtcaa tttctagcgc tgtttgagac gtttcagtgg actatagtaa
     2161 aatgaaataa gaataggaca aatcgatcag gacagtcaaa tcgatttcta agaatgtttt
     2221 agaagtaaag gtgtactatt ctagtttcaa tctattatat ttcagattcg gtccactatt
     2281 ccttcccaaa aacattgcat attgcgccga aggaattgct cacactatcc caagcacaaa
     2341 accctcagtc gtttgaccga gggttccttt ttattattca agaacttgat tagctcaatg
     2401 catccaaggc gtcatccatt gaaagaactt cgtggaagac acgttgtgtc aattcagttt
     2461 tttgttctgg agtgaggtat ttagtgttta cacagtatcc agagatacgt acgataacgt
     2521 cttcgcctga catgattttt tcgtaaacat cgttcaagtc cataacgttc aagttaacgt
     2581 gttgtccacc gttttcgaag taaccatcaa ggattgttac caagttatca acttgttcat
     2641 cacgagtctt accaagagca cgaggtgata cttgtgtagt caatgagata ccgtcagctg
     2701 cataactaaa gtcaaggcta gaaagtgagt tcaagttttg caaccaacca cctttagctt
     2761 tgttagatgg gttagcacct ggtgagaaga attcaagttt agacaagttc acagaaccat
     2821 cttcgttgag gtatacacct ttgtgaactg gtgagttacc agtttgttta gagtaagcaa
     2881 cgttagatgt gattgtcaaa agtgatactg tagcttctgc gtctttgtat agtttgtggc
     2941 tacgtagacg agttgtgtaa gcttcgatca accattctgc caattcgttt gaacgtgggt
     3001 catcttcacc ccagcgtggg tagtcaccga ttgtttcgta atcgtagatg tagccatctt
     3061 cgtcacggat tggtttaact gtagcgtatt tgatagctga caatgtatca acagtgttag
     3121 caaatccaca gataccgaat cccatgttgg cacgttgttt agttggcaag aaggccattt
     3181 gaacagcttc gtagttgtac ctatcagtca tgtagtggat gatgttcaag gcatctacgt
     3241 aagtgtcagt caaccagtca agagattttt caaagttcgc tttaactgat tcgaattcaa
     3301 gaacttcgtc acggattggt tcgatatcaa atactttgta gtctttgtga acatcgtcgt
     3361 aaccaccatt caaaccagta agaagggctt taagaacgtt tacacgagca ccgaagtact
     3421 ggatgttgtg gcgttgttct tcattttctg gatcaagtgg agacacacag catgagatac
     3481 agctcatttc accatatccg tctttagcca ttgttgttac accttcgtat tggatagaag
     3541 agtgtttgtg gctcatgtgc atacagtagc gacggaagtt gtatggcaat ttgtcagtcc
     3601 aaagaactgt caagtttggt tctggtgagt taccgatgtt gtcaagagtg ttcaagaaac
     3661 ggtagtccat cttagtaaca cggtgacgac cgtcgttacc cataccagcc atagaagttg
     3721 tgataaaggt tgggtcacct gagtacaatt ggtcataagc ttttgtacga gcaaatttaa
     3781 ctgtacgaag tttcataacg aaatcatcaa cgaattcttg gatttctgat tcagtaaatg
     3841 taccacgagc aaggtcacgt tctgcaaaga tgtccaatac gattggtaca cgacctagag
     3901 atgtagcagc accgttaatc acacggcaga cagccatgaa agcaatgtta acccattgga
     3961 ttgcttcttt cacgttcatc gctggtttgc gaacatcaac cccgtaaagg tcacccaggc
     4021 gaacaacttg ttgcaatgct tggtattgaa ggtttacttc ttcacgaaga cggattgttt
     4081 cttcatcgat ttctttgatt gcattccagt cgtttacttt ttcttgcatc aagtagtctg
     4141 caccgtaaag agcaagacgt gcgtaaacac cgatgatacg tccgcgtgag tatgcatctg
     4201 gaagaccagt tacagtgtgt gcgtgacgag cgcgacgaat atttgaagtg taggcacgga
     4261 aaataccgtc gttaactgtt gttacatatt tagtgaagat ttcgtgaaca gctgggtctg
     4321 gttcgtatcc attttctttc aaagtagttt cagccatacg gataccacct tttggcatga
     4381 agttcaattt gaagagttca tcgttttgga taccgaagat aacttcattt tctttgtcga
     4441 taaatccagc agggatatca gcgatagatg ttggacgagt gtccattggg aaacgagttt
     4501 cttcgtagtg tgctttagtt tcttctacaa ttttcttgat gtgaagtgaa cgctctgttg
     4561 gtcctgcaag gaagctttcg tctccatcat aaggtgtgta gttagcttgt acaaatcgtg
     4621 atacacttgc tttttctttc caatctacgc ctttgaagcc ttcccaagct ttgtcaaaaa
     4681 tatcttgtgc ttcaacaact gtcttaacaa ccatattaat gtcctctttt ttctagtaac
     4741 agtcatctgt tacattcata agacaagtat accatatagt aaccgatttc aacaagtgaa
     4801 aattccctat ttttacactt tcttttctaa aacagtctat att
//
LOCUS       DAVMEI010000122         4787 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVMEI010000122 DAVMEI010000000
VERSION     DAVMEI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.122"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            84..1259
                     /gene="mutY"
                     /locus_tag="VYQ24_001612"
     CDS             84..1259
                     /gene="mutY"
                     /locus_tag="VYQ24_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1968730.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1314..2018
                     /locus_tag="VYQ24_001613"
     CDS             1314..2018
                     /locus_tag="VYQ24_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1968731.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2011..3360
                     /gene="vicK"
                     /locus_tag="VYQ24_001614"
     CDS             2011..3360
                     /gene="vicK"
                     /locus_tag="VYQ24_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1968732.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVILLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3362..4171
                     /locus_tag="VYQ24_001615"
     CDS             3362..4171
                     /locus_tag="VYQ24_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1968733.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4262..4504
                     /locus_tag="VYQ24_001616"
     CDS             4262..4504
                     /locus_tag="VYQ24_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1968734.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMIHDLGLKDE"
     gene            4506..4760
                     /locus_tag="VYQ24_001617"
     CDS             4506..4760
                     /locus_tag="VYQ24_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1968735.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
BASE COUNT         1466 a          833 c         1095 g         1393 t
ORIGIN      
        1 atagaaaagt tatatactat ctattgaaaa tattataaat cagatacaaa gacaggtgaa
       61 accttctgtg ttatactaga gatatgttag atttgaaaga atacggtatc gtcatgtggc
      121 cggaggagaa agtcatttct ttccgtgaga aacttctcgc ttggtatgat gaaaacaaaa
      181 gagatttgcc ttggaggaga agtaaaaatc cttatcacat ctgggtatct gaaatcatgc
      241 ttcagcagac cagggtggat acagttatcc cttactacga aagatttttg gactggtttc
      301 caactgtcga aagtctggca actgcgcctg aggagagttt actgaaagct tgggagggct
      361 tgggctatta ttctcgagtt cgcaatatgc aggctgcagc ccagcagatt atgactgact
      421 ttggtggcca atttccaaat acctatgaag gaatttccag cttgaaaggg attggacctt
      481 acacagcagg agccatttcc agtattgctt ttaacttgcc tgagccagct gtagatggta
      541 atgtcatgcg ggtcttggcg cgtctgtttg aagtcaacca cgatattggg attccaagta
      601 atcgaaaaat ttttcaggca atgatggaga tcttgattaa tccagatcgt ccgggtgatt
      661 ttaatcaagc cttgatggac ttagggtctg atattgagtc tcctgtaaat cccagacctg
      721 aagaaagccc agtcaaggac tttagtgcgg catatcagaa tggaaccatg gaccgttatc
      781 caattaaatc tcctaagaaa aagccggttc ctatttatct taaagcctta gttgtgaaaa
      841 attctcaagg acaattttta cttgaaaaaa atgaaagcga aaagctcttg gcaggttttt
      901 ggcatttccc ctttatagaa gttgataact tttcgcaaga agagcagttt gacctctttc
      961 atcaggttgc ggaagaaagt gtgaactctg gccccagtcc agaagagagt ttccagcagg
     1021 actatgacct agatgttgat tggcttgatg tttgttttga tacagtccag catgttttta
     1081 gtcatcgaaa atggcatgta cagattgtag caggtcaggt gagtgacttc catgattttt
     1141 cagataggga agttcgttgg ctttcaccag aagaattcaa gaattaccca cttgccaaac
     1201 cccaacaaaa aatctggcag gcttatgcac aagccaactt agacagtagc caagactagc
     1261 tattgtgtct tctgactatt ttttggtata atagtagaga aaaaggtgaa catatgaaaa
     1321 aaatactaat tgtagatgat gagaaaccaa tctcggatat tatcaagttt aatatgacca
     1381 aggaaggtta cgaagttgta actgctttta atggtcgtga agcgctagag caatttgaag
     1441 cagagcaacc agatattatt attctggatt tgatgcttcc agaaattgat ggtttagaag
     1501 ttgctaagac cattcgtaag acaagcagtg tgcccattct tatgctttca gccaaagata
     1561 gtgaatttga taaggttatc ggtttggaac ttggggcaga tgactatgta acgaaaccct
     1621 tctccaatcg tgagttgcag gcgcgtgtta aagctcttct gcgtcgttct caacctatgc
     1681 cagtagatgg gcaggaagca gatagtaaac ctcaacctat ccaaattggg gatttagaaa
     1741 ttgttccaga cgcctacgtg gctaaaaaat atggcgaaga actagactta acccatcgtg
     1801 aatttgagct tttgtatcat ttagcatcgc atacaggtca agtcatcacg cgcgaacact
     1861 tgcttgagac tgtctggggt tatgactatt ttggtgatgt ccgtacagtt gatgtgactg
     1921 tacgacgtct gcgtgagaag attgaagata cgcccagccg accagagtat atcttgacgc
     1981 gccgtggtgt agggtattac atgagaaata atgcttgatt tactgaaaca aaccattttt
     2041 accagagatt ttatctttat cctgattttg ttaggtttca tccttgttgt gatcctctta
     2101 ttactggaaa atagacgtga taatattcag ttgaagcaaa tcaatcaaaa ggttaaagat
     2161 ttgattgcag gagattattc caaggttctt gatatgcaag gtgggtctga aatcaccaat
     2221 attaccaata atttgaatga cttgtcggag gttattcgtc tcactcagga aaatctagaa
     2281 caagagagta agaggctaaa tagtattctg ttttatatga cagatggggt tcttgcgacc
     2341 aaccgtcggg gtcagattat catgattaac gatacagcca agaagcaact ggggttggtt
     2401 aaggaagatg ttctgaatag aagcattttg gaattgctca agatagaaga aaactatgaa
     2461 ttgcgtgatt tgattaccca aagtccagaa ttgttgctag attcccaaga tatcaatggc
     2521 gaatatttga accttcgagt tcgctttgcc ttgatacgtc gagagtctgg ctttatttca
     2581 ggtttggtgg ctgttttgca tgatacgacg gagcaggaga aggaagaacg cgaacgaaga
     2641 ctctttgttt ccaatgttag ccatgagtta cggactcctc tgactagcgt aaaatcctat
     2701 cttgaagcct tggatgaggg ggctttgtgt gaaactgtag caccagactt tatcaaggtt
     2761 tctcttgatg agaccaaccg tatgatgcgc atggtgacgg atctcctcca tctttcacgt
     2821 attgataatg ctaccagtca cctagatgtg gaactgatta acttcactgc ttttattacc
     2881 tttatcctca atcgttttga caagatgaaa ggacaggaaa aggagaaaaa atatgagttg
     2941 gtgagagatt atcccatcaa ttctatctgg atggaaattg atacagataa gatgacgcag
     3001 gttgtcgaca atattttaaa taatgctatt aagtattcgc cagatggggg taaaatcact
     3061 gtcagaatga agacaactga agaccagatg attttatcca tttctgacca cggtttgggg
     3121 attcctaagc aggatttacc acgtatcttt gaccgtttct atcgtgtgga tcgtgctaga
     3181 agtcgtgcac aaggtggtac aggtctagga ctgtctatcg ctaaagaaat tatcaaacaa
     3241 cataagggct ttatttgggc caagagtgaa tacggcaagg gttcaacctt taccattgtg
     3301 ctcccttatg ataaggatgc agtgaaagaa gaagtatggg aggatgaagt agaagactag
     3361 aatgagtgaa ataggcttta aatacagtat tttagcgtcg ggttccagtg gaaattcttt
     3421 ttatctggaa acctcaaaaa agaagctttt agtagatgca ggcttgtctg gcaagaaaat
     3481 taccagtctg ctagctgaaa ttaaccgtaa gccagaagac ctggatgcca tcttgattac
     3541 ccatgagcat tcagatcata tccatggagt aggcgttttg gctcgcaagt atggtatgga
     3601 tctttatgcc aatgaaaaga cctggcaagc tatggaaaat agtaaatatc ttggcaaggt
     3661 ggattcttcg caaaagcata tttttgaaat gggtaaaacc aaaacctttg gagatatcga
     3721 catcgagagt tttggtgtaa gccatgatgc agtcgcaccg cagttctatc gctttatgaa
     3781 ggatgataag agttttgtcc tcttgacaga tacaggttat gtcagtgacc gtatggcggg
     3841 cattgtcgaa aatgcggatg gttatcttat cgaggccaac catgatgtag agattttgcg
     3901 atcaggttct tacgcttggc gactcaaaca acgaatccta tctgaccttg gtcacctttc
     3961 taacgaggac ggtgctgaag ctatgattcg gacgctagga aatcgtacta agaagattta
     4021 ccttgggcat ttatctaagg aaaacaatat caaggaactg gctcatatga ccatggtcaa
     4081 tcagctggct caagctgatc tgggagtcgg agtagacttt aaggtttatg atacctcacc
     4141 agataccgca acaccattga cagagatata gaaagaacgc tgagaaggtg ttctttttat
     4201 attgactgga cacctaaaaa gtaatacaat ggtgttacca ttaaaaaagg gagcacaaaa
     4261 gatgactact attacattaa aagtttctga agctgataaa acatttatga aagcaatggc
     4321 taagtttgaa ggagtttccc tttcggaact tattcgcacc aaaactcttg aagccctaga
     4381 agatgaatac gatgctcgtg tggcagattt agcctatcaa gagtatttag aagacttgga
     4441 aaaaggagtt gaacccatta cttgggaaga aatgatccat gatttaggct tgaaggatga
     4501 ataatttgta taaattagtt ccaacaagac gttttatcaa gcaattgaaa aaattggacc
     4561 gttatacgca gaagctaatt acaaactatt tacaaaccaa tgttttggaa gacccaagac
     4621 gacacggaaa ggctttggtt ggtaatcgcg ttggtcaatg gcgctataga attggtaatt
     4681 atcgagttat cgtacaaatt gtagatgatg aattagtcgt tgctactcta gaagttggtc
     4741 atcggagaga tatttattga attacttttt tcaagtaaat tttcatt
//
LOCUS       DAVMEI010000123         4729 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVMEI010000123 DAVMEI010000000
VERSION     DAVMEI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.123"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            28..243
                     /locus_tag="VYQ24_001618"
     CDS             28..243
                     /locus_tag="VYQ24_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968736.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETAVGLIDFYEKNYEKFED"
     gene            complement(352..1092)
                     /locus_tag="VYQ24_001619"
     CDS             complement(352..1092)
                     /locus_tag="VYQ24_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1968737.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(1092..2657)
                     /locus_tag="VYQ24_001620"
     CDS             complement(1092..2657)
                     /locus_tag="VYQ24_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1968738.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            2795..4684
                     /locus_tag="VYQ24_001621"
     CDS             2795..4684
                     /locus_tag="VYQ24_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1968739.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRGA
                     KAADEKRIQARGIQLKSSFEAVLKEMQEGVRKRVVFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
BASE COUNT         1310 a          863 c         1104 g         1452 t
ORIGIN      
        1 ttataaagaa gaaaatagga gaaaaacatg gctaagtcaa actttgaaaa agtagaatca
       61 gttgttggct gggttcgtga taagaaaatc acaggctacc gtatctctaa agaaacgaat
      121 gcgcgtgaaa tgtctatcat tgctctggcg cagggtcgtg caaaagtaaa aaatatttca
      181 tttgaaacag ccgtaggctt aattgatttc tatgaaaaaa attatgaaaa atttgaagat
      241 taatctttgg ataacggcgg attcttgacc ttcaagtagt agagatagag aatctgcctt
      301 ttcattttga ggacagcaaa aagactgcac ggttgatgca gccttttctt tttatttgag
      361 atagcgttga aggaactctt ttgttcggtc ttctttagga ttggtgaaga ggtcttctgg
      421 tttaccttct tcagcgatca cgcccttatc cataaagata acacggtgag agacatcacg
      481 ggcaaattcc atttcatggg ttacgacaat catggtcaag ccttcctgag ccaggtcctg
      541 catgattttg aggacttctc caaccatttc tggatcgaga gctgatgttg gttcatcaaa
      601 gagaatagcg tccggattca tggagagggc acgagcgatg gccacacgtt gtttttgacc
      661 acctgagagt tgttttggtt tggcttgcca gtagcgttct cccatgccga ccttttccag
      721 gttttctttg gcaatctttt cagcttctgt gcgttcgcgt tttaggacag ttgtctgagc
      781 gacgattgtg ttttcaagaa cattgagatt ttcaaagagg ttaaaggatt ggaaaaccat
      841 ccccaacttt tcacggtatt gcgtgaggtc atagcctttt tcgaggacgt tttgtccatg
      901 ataaaggatt tgtccatcag ttggtgtttc aagtaggtta atggagcgta ggaaggtcga
      961 ttttccgctt ccagagcttc cgatgataga gatgacctct cccttgtgga cagtgagtga
     1021 aatgtctttt agcacttcgt tttgtccata ggattttttg aggtgtttaa tttcaaggat
     1081 tgcttgtgtc attatttcaa atcctccgtt tgcatttggt tagcacctgt agtataggta
     1141 tccatgtcca ttcggcgctc gataaatcgt aggatacgtg ttacggtgaa ggtgaggaca
     1201 aagtaaatca cggcgatgat tgtaaatgtc tggaagtatt gataggtttg tgttgccacg
     1261 gtatttcctg agaaataaag ttcgacaaca gagataacgt tcaatacaga tgtatctttg
     1321 atattgatga caaattcatt accagttgca ggtaggatgt tacggactac ctgaggtagg
     1381 acaatcttac gcatggtctg gttatgggtc ataccaagag cagtcgcagc ttcaaattgt
     1441 cccttgtcaa ctgctaggat accaccacgg acgatttcag tcatgtaggc accggtattg
     1501 attgaaacga tgaagatagc agccagtgta cggtcaaggt tgatcccgaa agcttgggca
     1561 gttccatagt agataaccat cgattgaaca atcattggcg taccacggaa aatttcaatg
     1621 tagacattga gaacccagcc gactagtttt tgaaggccat aaatggcttt gttttcagag
     1681 agaggagcag tacggaagac accaatggca agtccaataa tgagacctat gatggttccg
     1741 acgatagaga ttaaaagagt gataccagca ccacgcaaga gttgttgcca gttttcagaa
     1801 agaattttag cgacttggct aaagaaacta ctgctagtct cttcagttgt tgtagcttcg
     1861 gcaggttgtt ccttgatcat acgatccatc aaggcaactt ggtcatcttt tgaaatggtt
     1921 tcaatgctgg cattgatttg gctaatacga ttgtcatttt tacgaagccc gatagcgata
     1981 gctgtatctt cttccccagt tttgaaacca ggttctactt gaatcatctt gaacttagag
     2041 ttcgcagctt cagcagtcag tgcttctgga cgttcagaaa cataagcatc aatgacacca
     2101 gcctcaagag cttggcgcat ttgagcgaag tctcccatgg ctgtttcttt tttagcacct
     2161 gggatttgtg caatcaagtc gtagaggtag acaccttgtt gagaagtgat tttcgcacca
     2221 ttaaagtcat ccaaagattt agcacttgcg taggcagaat cttttttgac aagcaaaact
     2281 ggttcgctag tgtagtaact gctcgaaaag gcaatttctt gtttgcgttc tgcagttgga
     2341 ctcatacctg cgataatcat gtcaatctta ccagaagtaa gggcagggac tagaccttcc
     2401 cacttggttt taacaaccaa aggttcttta cctaagtcct tagcgatttt cttggcgatt
     2461 tgaacatcgt atccgttggc atactgattg gtcccatcga ttttgacagc tccgttgcta
     2521 tcatcatcct gggtccagtt aaagggagca tatgctgctt ccatcccgat gcgtaaatat
     2581 tcatcggctt gagcaacatt gacaagtcct agcatcagca agagacttgt gaaaatagat
     2641 aagtatattt ttctcatgat ttctcctatt ctgatctatt aaaaaataac tgtctcctat
     2701 tttatcgaaa aatgcgtaat ttttcaacat aagtaagcct ttacttacga aaaaatgcta
     2761 taatgataag aaagataaaa agggggctta gttgatgaaa aaaacttttt tcttactggt
     2821 gttaggcttg ttttgccttc ttccactctc tgtttttgcc attgatttca agataaactc
     2881 ttatcaaggg gatttgtata ttcatgcaga taatacggca gagtttagac agaagatagt
     2941 ttaccagttt gaggaggact ttaagggaca aatcgtggga cttggacgtg ctggtaagat
     3001 gcctagcggg tttgacattg accctcatcc aaaggttcag gccgcgaaaa acggtgcaga
     3061 actagcagat gtgactagcg aagtaacaga agaagcggat ggttatactg tgagagtcta
     3121 taatccaggt caggagggcg acatagttga agttgacctc gtctggaact taaaaaattt
     3181 acttttcctt tatgatgata tcgctgaatt aaattggcaa cctctgacag atagttcaga
     3241 gtctattgaa aagtttgaat ttcatgtaag gggagacaag ggggctgaaa aactcttttt
     3301 ccatacaggg aaacttttta gagagggaac gattgaaaag agtaaccttg attatactat
     3361 ccgtttagac aatcttccgg ctaagcgtgg agttgagttg catgcctact ggcctcggac
     3421 cgattttgct agcgctaggg atcagggatt gaaagggaat cgtttagaag agtttaataa
     3481 gatagaagac tcgattgtta gagaaaaaga tcagagtaaa caacttgtta cttgggtcct
     3541 cccttcgatc ctttctatct ccttgttatt gagtgtctgc ttctatttta tttatagaag
     3601 aaagaccact ccttcagtca aatatgccaa aaatcatcgt ctctatgaac caccaatgga
     3661 attagagcct atggttttat cagaggctgt ctactcgacc tccttggagg aagtgagtcc
     3721 cttagtcaag ggggctggca aattcacttt tgaccaactt attcaagcta ccttgctaga
     3781 tgtgatggac cgtgggaatg tttctattat ttcagaagga gatgcagttg gcttgagatt
     3841 ggtgaaagaa gatggtttgt caagatttga gagagactgt ctaaatctag ccttttcagg
     3901 caaaaaagaa gccactcttt ccaatttgtt tgcggattac aaggtatctg acagccttta
     3961 tcgcggagct aaagctgctg atgagaaacg gattcaagca agagggattc agctcaaatc
     4021 ttctttcgaa gcagtattaa aagagatgca agaaggagtg agaaagcgag ttgtcttctg
     4081 gggactccca gattactatc gtcctttaac tggtttggaa aaggctttgc aagtgggtat
     4141 gggtgtcttg actatcttgc ccctatttat cggatttggt ttgttcttgt acagtttaga
     4201 cgttcatggc tatctttacc tccctttgcc aatacttggt tttctagggt tagttttgtc
     4261 tgttttctat tattggaagc ttcgactaga taatcgtgat ggtgttctaa atgaagcggg
     4321 agctgaggtc tactatctct ggaccagttt tgaaaatatg ttacgtgaga ttgcacgact
     4381 ggataaggct gaattggaaa gtattgttgt ttggaatcgt ctcttggtct atgcaacctt
     4441 atttggctat gcggacaagg ttagtcattt gatgaaggtt catcagattc aagttgaaaa
     4501 tccagatatc aatctctatg tagcttatgg ctggcacagt atgttttatc attcaagcgc
     4561 gcaaatgagc cattatgcta gtgtcgcaaa tacagcaagt acctactctg tatcttctgg
     4621 aagtggaagt tctggtggtg gcttctctgg aggcggaggt ggcggcagta tcggagcctt
     4681 ttaaagagag ctaccataga ctgaaaaagt atgatataat ggaagatag
//
LOCUS       DAVMEI010000124         4654 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVMEI010000124 DAVMEI010000000
VERSION     DAVMEI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.124"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            143..902
                     /locus_tag="VYQ24_001622"
                     /pseudo
     CDS             143..902
                     /locus_tag="VYQ24_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            942..1706
                     /locus_tag="VYQ24_001623"
     CDS             942..1706
                     /locus_tag="VYQ24_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1968740.1"
                     /translation="MISLFGLVAAKPVQANTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1719..2396
                     /locus_tag="VYQ24_001624"
     CDS             1719..2396
                     /locus_tag="VYQ24_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1968741.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPFYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPGFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2406..3065
                     /locus_tag="VYQ24_001625"
     CDS             2406..3065
                     /locus_tag="VYQ24_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1968742.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALVEI
                     IRGGLNAVDKGQYEVGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLVKRWQQAA
                     E"
     gene            3360..4550
                     /locus_tag="VYQ24_001626"
     CDS             3360..4550
                     /locus_tag="VYQ24_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1968743.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKIIHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
BASE COUNT         1318 a         1081 c          837 g         1418 t
ORIGIN      
        1 attgttagaa atcgatttga ttgtcccgat cgatttttcc tgttattata tcactatagt
       61 gaattttttc agatatgatt tgacaagaac attcttttat cgtagaatca tgttataaaa
      121 tctaacacaa gggagatctc tcatggcttt agtagaattt aaaaacgtcg aaaaatatta
      181 cggagactac cacgcactcc gcaacatcaa tctccgtttt gaaaaaagga caagttgttg
      241 tcctgcttgg accttctggc tctgggaagt ccactcttat ccgtacgatc aatggtttag
      301 aggctgttaa caaaggaagt ctcctagtca atgggcacca agttgctggt gccagccaga
      361 aagatttggt acctcttcgc aaggaagtcg gcatggtttt tcaacatttt aacctttatc
      421 cacacaaaac agtgttagaa aacgtaacac ttgcacccat tgaagttcta ggaattgata
      481 aaaaagaagc tgaaaaaacc gcccaaaaat atctggaatt tgtaaatatg tgggacaaga
      541 aagattccta tcccgccatg ctatctggtg gacaaaaaca gcggatcgcc atcgctcgtg
      601 gtcttgctat gcatccggaa ctcctcctct ttgatgaacc aacatctgct cttgatcctg
      661 agactatcgg agatgttcta gcagttatgc agaaactggc gcatgatggg atgaacatga
      721 tcatcgttac ccacgaaatg ggctttgctc gagaggttgc ggaccgcatt atctttatgg
      781 ccgacggaga agttttagta gatatgacag atgtcgatag cttttttgac aatccaagcg
      841 aacctcgtgc ccaacaattc ctcagcaaaa ttatcaacca cgaaagtgac aaagtcaaat
      901 aaggaggcgc ctatgaaaaa aaattctttt tatcagcatt attgattagc cttttcggcc
      961 ttgttgctgc caaaccagtc caggctaata ctagtatcgc agacattcaa aaaagaggcg
     1021 aactggttgt cggcgtcaaa caagacgttc ccaattttgg ttacaaagat cccaagaccg
     1081 gtacttattc tggtatcgaa accgacttgg ccaagatggt agctgatgaa ctcaaggtca
     1141 agattcgcta tgtgccggtt acagcacaaa cccgcggccc ccttctagac aatgaacagg
     1201 tcgatatgga tatcgcgacc tttaccatca cggacgaacg caaaaaactc tacaacttta
     1261 ccagtcccta ctacacagac gcttctggat ttttggtcaa taaatctgcc aaaatcaaaa
     1321 agattgagga cctaaacggc aaaaccatcg gagtcgccca aggctccatc acccaacgcc
     1381 tgattactga actgggcaaa aagaaaggtc taacctttaa atttgtcgaa cttggttcct
     1441 acccagaatt gattacttcc cttcacgctc atcgtattga tgccttttcc gttgaccgct
     1501 ctattctgtc tggctacact agtaaacgca cagaactact agatgatagt ttcaagccat
     1561 ctgactacgg tattgttacc aagaaatcaa atacagagct caacgactat cttgataact
     1621 tggttactaa atggagcaag gatggtagtt tgcagaaact ttatgaccgt tacaagctca
     1681 aaccatctag ccatactgca gattaaggag gacaccccat gacagattta tcatcttgga
     1741 cagcctattt tcaggatttt ggacaatttt tcaatggttt cctctttact cttgccctag
     1801 cgattggatc ctttatcctc gccatggtct taggaatctt ctttggtgcc atgtcaacca
     1861 gtaaacgtcc aattttacgc attttagctc gcatctttgt tgaattttac caaaatactc
     1921 ccctcttggt gcagtttgtt attgtttttt atggcctacc tcttatcagt gaccatatca
     1981 tcatgattcc attttattgg acagccgttc tctgcgtggg actctatcac ggcgcttata
     2041 tcgctgaggt tattcgttca gggattcagt ctattcctag tggtcagatg gaggccgcct
     2101 tgtcgcaagg ttttacctat atcagtgcca tgcgcttgat tatcttgcct caggccttcc
     2161 gcatcattct ccctccattg accaaccaaa ttgttaacct catcaagaac acctctactg
     2221 tagctatcat ctctggagta gacttgatgt ttgtgactaa gtcttggtcg gctctcaacg
     2281 gaaactatat cccaggcttt ttaggtgctg ctcttctcta ctttgcccta tgcttccctg
     2341 ttgcccagtt tggtcgcaag atggagcaag ccaacaaaaa agcctattca ctttaggagg
     2401 ttactatgga atctatttta gaagttttaa ccccagataa cctagtcttt atctttaaag
     2461 gatttggctt gaccctctat atttctctga ttgccatcat cctctctact atcatcggta
     2521 cggtgctagc tgtcatgaga aatggcaaaa atcctgtctt gcgaatcatc tccagtattt
     2581 atatcgagtt tgtgcgcaac gttcccaacc ttctctggat ttttactatc tttttggtgt
     2641 tcaaaatgaa atccacacca gcaggtatta cagcctttac tctctttaca tcagcagcct
     2701 tggttgagat tattcgaggc ggtctcaatg ccgtagacaa gggacaatac gaagtaggaa
     2761 tgtcacaagg cttcacctca gcccaaatcc tctactacat cattctccca caagccatcc
     2821 gcaaaatgct accagccatc atttctcagt ttgttaccgt gattaaggat accagtctcc
     2881 tctactctgt tatcgcccta caagaactct ttggagccag ccaaattctc atgggccgtt
     2941 atttcgaacc agagcaggtc ttcagtcttt acatcctgat tgccctcatc tacttcagct
     3001 ttaacctagc aatttctagc ctgtctcata tgctagtcaa acgttggcaa caagctgcag
     3061 aataatactc ttcgaaaatc tcttcaaacc acgtcagctt taccttgccg tatatatgtt
     3121 actgacttcg tcagttctat ccacaacctc aaaacggtgt tttgagcagc ctgcggctag
     3181 cttcctagtt tgctctttga ttttcattga gtataaacag ccactctcca gttcaattgg
     3241 agagtttttt gatgatagta gatatttata gacctcaatt tgacaccata cattttctat
     3301 gataaaatgt aacaaattta tttacaacaa atcgaaatct aaatgaaatg gaattttcta
     3361 tgaaatcagt caaaggacta ctctttatca tagctagttt tatcttgact cttttgactt
     3421 ggatgaacac ttctccccaa ttcatgattc caggactagc tttaacaagc ctatctctga
     3481 cttttatcct agccactcgt ctcccactac tagaaagctg gtttcacggt ttggagaagg
     3541 tctacaccgt ccacaaattc acagcctttc tctcaatcat cctactaatc tttcataact
     3601 ttagtatggg cggtttgtgg ggctctcgct tagctgctca gtttggcaat cttgccatct
     3661 atatctttgc cagcatcatc cttgtcgcct atttaggcaa atacatccaa tacgaagctt
     3721 ggcgatggat tcaccgcctg gtttacctag cctatatttt aggactcttt cacatctaca
     3781 tgataatggg caatcgtctc cttacattta atcttctaag ttttcttgtt ggtagctatg
     3841 cccttttagg cttactagct ggtttttata tcatttttct atatcaaaag atttccttcc
     3901 cctatctagg gaaaattatc catctcaaac gcttaaatca cgatactaga gaaattcaaa
     3961 tccatcttag cagacctttc aactatcaat caggacaatt tgcctttcta aagattttcc
     4021 aagaaggctt tgaaagtgct ccgcatccct tttctatctc aggaggtcat ggtcaaactc
     4081 tttactttac tgttaaaaat tcaggcgacc ataccaataa tatctatgat aatcttcaag
     4141 ccggcagcaa agtaacccta gacagagctt acggacacat gatcatagaa gaaggacgag
     4201 aaaatcaggt ttggattgct ggaggtattg ggatcacccc cttcatctct tacatccgtg
     4261 aacatcctat tttagataaa caggttcact tctactatag cttccgtgga gatgaaaatg
     4321 cagtctacct agatttactc cgtaactatg ctcagaaaaa tcctaatttt gaactccatc
     4381 taatcgacag tacgaaagac ggctatctta attttgaaca aaaagaagtg cccgaacatg
     4441 caaccgtcta tatgtgtggt cctatttcta tgatgaaggc acttgccaaa cagattaaga
     4501 aacaaaatcc aaaaacagag cttatttacg aaggattcaa gttcaaataa gtaatcatac
     4561 tctcctggga tttcccagga gaatttttta tagccaaatg aaataagacc ggaacgatgg
     4621 aattttggca ttcaaatcaa ttgataagaa tatt
//
LOCUS       DAVMEI010000125         4632 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVMEI010000125 DAVMEI010000000
VERSION     DAVMEI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.125"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(192..1178)
                     /locus_tag="VYQ24_001627"
     CDS             complement(192..1178)
                     /locus_tag="VYQ24_001627"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1968744.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1372..3840
                     /gene="gyrA"
                     /locus_tag="VYQ24_001628"
     CDS             1372..3840
                     /gene="gyrA"
                     /locus_tag="VYQ24_001628"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1968745.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3840..4583
                     /gene="srtA"
                     /locus_tag="VYQ24_001629"
     CDS             3840..4583
                     /gene="srtA"
                     /locus_tag="VYQ24_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1968746.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         1420 a          814 c         1031 g         1367 t
ORIGIN      
        1 aggaaaattg atttatagaa atgttttagt agtcgatgcg tactgttata gattcaacga
       61 actataaaat ctgaagaaag caattttaac cagatcttag aagcagttgt gtactactct
      121 aacttcaata gactatatca tatagataaa aaacaactcc ctgatgattc aaggagttgt
      181 ctatagttaa attagttttt agaagcttct tggaattctg gatttttcca tgcttcgtca
      241 ataatagctt gcaattcttt agcagatgct tgcatttttt gagtttctgc atcgttcaat
      301 ggaatgttta ctggacgaac gataccatgt gcaccaacaa cagctggttg accgataaag
      361 acattctcaa ctccgtattg accttcttgg aatactgaaa gtggaagtac tgcgttttca
      421 tcgtcaagga ttgctttagt gatacgagca agggctactg cgataccgta gtatgttgca
      481 ccttttttgt tgatgattgt gtaggctgca tcacgaacac cttcgaacaa ttcaatcaat
      541 tcagcttctt gaacattttg agtgtcttta aggaattctt caaggtttac accagcgatg
      601 ttagcgtgtg accaaacagc gaactcagag tcaccgtgtt cacccatgat gtaggcgtgc
      661 actgaacgag catccacatc caatttttca gcaagtgctt gacggaaacg agctgagtca
      721 agtgaagtac ctgaaccgat aacgcgttct ttagggaaac cagagaattt ccaagttgag
      781 taagtcaaaa cgtcaactgg attagcagca acaaggaaga tacctttgaa accagattca
      841 acaacttgag ttacgattga tttgttgata gcaaggtttt tacctacaag gtcaagacga
      901 gtttcacctg gtttttgagg tgcacctgca gtgatcacaa caaggtcagc gtctgcacag
      961 tcagagtatt gagctgcata gattttttta ggtgaagtga aggcaagggc gtgactaagg
     1021 tcaagcgcat caccaacagc tttttcatgc aattgtggaa tttcgataat tccaagctct
     1081 tgtgcaattc cttggttaac aagtgcaaaa gcgtaagatg aacctacagc accatcaccg
     1141 acaaggataa cttttttgtg ttgtttagtt gaagtcattg ttttaaacat ctccttaatt
     1201 ttattagggg attttcccta gacaacttca ttctatcact tttaaaaaac tttgtcacga
     1261 atatgcctta tagttctcga tgtaaacgtt ttagtggttt agaggctgaa atagatggga
     1321 atttatggta taatgttgtt acttactaat tgtgaaatga ggcatttatt aatgcaggat
     1381 aaaaatttag tgaatgtcaa tctgacaaag gagatgaagg caagttttat cgactacgcc
     1441 atgagtgtta tcgtagcgcg agctcttcct gatgttcgag atggcttaaa acctgttcac
     1501 cgtcgcattc tctacggaat gaatgaattg ggtgtgaccc cagacaaacc ccataaaaaa
     1561 tctgctcgta ttacagggga tgtcatgggt aaatatcacc cacacgggga ttcctctatt
     1621 tatgaagcca tggtccgtat ggctcaatgg tggagctacc gttacatgct tgtagatggt
     1681 catgggaatt ttggttccat ggatggagat agtgctgccg ctcaacgtta taccgaggca
     1741 cgtatgagca agattgctct ggaaatgctt cgtgatatca acaaaaatac agttgatttc
     1801 gttgataact atgatgccaa tgaacgggaa cccttggtct tgccagcgcg ttttccaaac
     1861 cttttggtta atggagcaac tggtatcgcg gttgggatgg caaccaatat tccacctcat
     1921 aatctgggtg aaaccattga tgcagtgaag ttggtcatgg ataatcctga agtgactacc
     1981 aaggacttga tggaagtctt gcctggacca gattttccaa ctggtgctct tgtcatgggg
     2041 aaatcaggta tccataaggc ttatgaaaca ggtaaaggtt cgattgtcct acgttctcgt
     2101 acagagattg aaacgactaa gactggtcgt gagcgcatcg ttgtaacaga atttccttac
     2161 atggtcaata aaaccaaggt gcatgagcat attgttcgct tggttcagga aaaacgcatt
     2221 gagggtatca cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt tgttattgaa
     2281 gtcaagcgcg acgcctcagc caatgttatt ctcaataacc tcttcaaaat gacccaaatg
     2341 caaaccaatt ttggtttcaa tatgctcgct atccaaaatg gtataccgaa aattttgtct
     2401 cttcgtcaga ttttggatgc ttatatcgag caccaaaaag aagtggttgt tcgtcgtaca
     2461 cgttttgata aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct cttgattgcg
     2521 ctagaccata tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga tgcggaagct
     2581 caagctgagt tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc tatccttgat
     2641 atgcgtcttc gtcgtttgac aggtttggaa cgcgataaga ttcaatctga gtatgatgac
     2701 ctcttggctc tgattgcgga tttagcagat attcttgcta agcctgaacg tgtttctcaa
     2761 attatcaaag acgaattgga tgaagttaaa cgtaaatttt ctgataaacg ccgtacagag
     2821 ttgatggttg gacagatttt gagtctcgag gatgaggact tgattgaaga atcggatgtc
     2881 ttgattaccc tttctaacag aggctacatt aagcgtttgg atcaggacga gttcactgct
     2941 caaaaacgtg ggggtcgtgg tgtccaagga acgggagtga aagatgatga ctttgttcgt
     3001 gagttagtgt caactagcac acatgatcat ctgctcttct tcacaaacaa gggacgtgtc
     3061 tatcgtctta aaggttatga aattcctgag tatggtcgga ctgccaaagg gctaccagta
     3121 gtcaatctct tgaaattgga tgaagacgaa agtattcaga cggttatcaa tgttgagtct
     3181 gatcgcagtg atgatgctta tctcttcttt acaacccgtc acggtattgt gaagagaact
     3241 agtgttaagg agtttgccaa tattcgtcaa aatggtctca aagcgctgaa tttaaaggat
     3301 gaagatgagt taatcaatgt cttgttggca gaaggagata tggatattat cattggtacc
     3361 aagtttggtt atgcagttcg ctttaatcaa tcagccgttc gtggtatgag ccgtatcgcc
     3421 actggtgtga aaggtgttaa ccttcgtgaa ggagacacag ttgttggtgc cagcttgatt
     3481 actgatcaag atgaagttct tattatcaca gaaaaaggat atggtaagcg tacagtcgct
     3541 actgaatacc caacaaaagg tcgtggtggt aagggaatgc agacagctaa aattaccgaa
     3601 aaaaatggct tgctggccgg tcttatgact gttcaagggg atgaggattt gatgattatc
     3661 actgatacag gtgtcatgat tcgaaccaat cttgccaata tttcacaaac aggacgtgca
     3721 actatgggag ttaaagtaat gcgcctggat caagatgctc agatagtgac tttcacaacg
     3781 gttgcggtgg cagaaaaaga agaagttggg acagaaaacg aaacagaagg tgaagcataa
     3841 tgtctcaaaa aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca aatatcctag
     3901 caggatttct gatattactg tcactggctt tgatttttaa tactcaaatt cgaaatattt
     3961 tcatagtctg gaataccaat aagtatcaag ttagccaggt atcaaaagaa aaattagaag
     4021 aaaatcagga tacagaaggc aattttgact ttgattctgt caaagctatc tcttcggaag
     4081 ctgttctaac ttctcaatgg gatgctcaaa aattaccagt tattggggga attgcaattc
     4141 ctgaattgga aatgaatttg ccgattttta aaggacttga taatgttaat ctcttctacg
     4201 gagctggtac aatgaaacgc gagcaagtaa tgggagaagg aaattatagt ctagctagtc
     4261 accatatctt tggtgttgat aatgctaata aaatgttatt ttctccttta gataatgcta
     4321 aaaatggcat gaagatttat ctaaccgata aaaataaagt ttatacttat gaaatacgtg
     4381 aagtcaaacg tgtgacaccg gatcgtgttg atgaagttga tgatagagat ggggtcaatg
     4441 aaatcacatt agtaacctgt gaagaccttg ctgctacaga acgtattatt gtcaaaggtg
     4501 atttgaaaga aacaaaagat tattcacaaa catctgatga aatcctaaca gctttcaatc
     4561 aaccatataa acaattttat taatacaaat cagtgaaatc atggatttca ctgatttttt
     4621 aagattttca ta
//
LOCUS       DAVMEI010000126         4595 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVMEI010000126 DAVMEI010000000
VERSION     DAVMEI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.126"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..645)
                     /locus_tag="VYQ24_001630"
     CDS             complement(<1..645)
                     /locus_tag="VYQ24_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1968747.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNK"
     gene            complement(933..1895)
                     /gene="nrdF"
                     /locus_tag="VYQ24_001631"
     CDS             complement(933..1895)
                     /gene="nrdF"
                     /locus_tag="VYQ24_001631"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1968748.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(2083..4242)
                     /gene="nrdE"
                     /locus_tag="VYQ24_001632"
     CDS             complement(2083..4242)
                     /gene="nrdE"
                     /locus_tag="VYQ24_001632"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1968749.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDEEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWTELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4323..4541)
                     /gene="nrdH"
                     /locus_tag="VYQ24_001633"
     CDS             complement(4323..4541)
                     /gene="nrdH"
                     /locus_tag="VYQ24_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1968750.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         1336 a          857 c          946 g         1456 t
ORIGIN      
        1 cttattgaac ttactatgat ctgcgagtaa atatttttta ttagaattat ttaaagcgat
       61 gcgttgagcc tctccctctt cctcgctaaa agtagctaga gctccgtttt gaataccatt
      121 acagctaacg aaagctttag aaaattggag attagagaga ttttgtaggg tcaatgtgcc
      181 aacaaaagca cctgtaatat cgcgataatt tccacctatc aaaatcaaat ctgttaattt
      241 tcgttcgctt aaaatcagaa aaacaggtag actgttggtt acgacgcgga tattgtcaat
      301 aggcaactca cgcgcaaaaa actctaatgt tgttcctggt ccaatgaaaa tagtttctct
      361 ttcttctact agactgcctg caaaatgggc tatttcttgt ttttctgccg tttggagggc
      421 ttgtttttca atatttgatc gctcattagt caaaagggag ttggttcgaa gtttttcagc
      481 tccaccatgc acacgaatca gcaaatcttt atcagctaat tcctgtaaat agcgccttgc
      541 agtcatatct gaaacggcta tttcgtccat aatctgttta actgttatgg ttcctttact
      601 atttactatc tctaaaattt tggttaattt ttcttgtttg agcatattat cacctcgttt
      661 cctactacta tcttaccata aacaaactca tcattcaaat acaaaaacaa caaaatgaaa
      721 caaaaacaaa aatatcgaag tttgttttca aaactttcga tatttttgtt gggttattat
      781 tttgttgttt ctagtttact ttttgatggt taagagtggt ggagaattat actcaatgaa
      841 aatcaaagag caaactagga agctagccgc aggctgtact tgagtacggc aaggcgaagc
      901 tgacgtggtt tgaatttgat tttcgaagag tattagtcca aaccgtagtt gtagtcatca
      961 tcttgcatgg cttcaacttc gccaagaagg taaccatttc cgacttgaga gaagaagtca
     1021 tggttggaag ttcctgttga aataccgttc ataacgattg ggttgacatc ttcagctgaa
     1081 tctgggaaaa gtggatcttg tcccatgttc atgagagctt tattggcatt gtagcgaagg
     1141 aaggttttaa cctcttcagt ccaaccaaca ccgtcataaa gactctctgt gtagccttct
     1201 tcattttcat aaagagtata gagtaggtcg tacatccatt ctttgagttt ttcttgctct
     1261 tcttcaggta attcattgaa accaagttgg aatttgtaac caatgtaggt tccgtgaaca
     1321 gactcgtcac gaataatcaa tttaatgatt tctgcaacgt tggcaagttt gttgttaccg
     1381 agatagtaga ggggagtgaa gaaaccagag tagaagagga aggtttcgag gaagacgctg
     1441 gcaactttct tttcaagtgg gctgccgttt aggtagattt cgttgacaat ctcagccttc
     1501 ttttgtaggt aaggattggt attggtccat tcgaaaattt cttcaatctc agccttagta
     1561 ttcaaggtag aaaagattga tgagtaagat ttagcgtgga cagattccat aaattggatg
     1621 ttattgaaaa cagcttcctc atgtggtgta cggatgtctg cgcgaagggc ttgaacccca
     1681 gtttcagatt gcatagtgtc aagaagggtt aaaccaccaa aaacttttcc gaccaagtct
     1741 ttctctttgt tagatagctt tctccagtca tccaagtcgt ttgataaggg aatacgtgta
     1801 tcgagccaaa attgctccgt cagtttttcc caagttgatt tgtcgatgac atcttcgatg
     1861 gcattccagt taatggcttt gtagtaagtt tccatttaaa atctctttct gtgtttagta
     1921 ttgcgaactc acaattattt ctactttacc ataattctat aggagtatcg cacaaaaagt
     1981 cggaagcccg acttttaaaa tgttacataa attatgttat gacatagtag atttgatttt
     2041 atcagtgctg cttagggaaa aatagtgttt ctatgctaga aactaaatca cacagctttc
     2101 acattggttg gcgccgactt ctccaccgtc atctgtaaag gtacggacgt agtagataga
     2161 cttgattccc ttgttaaagg catagttacg aaggatggac aagtcacgtg tcgtttgttt
     2221 attttccctc ttccattcgt aaaggccttt tggaatgtca ctacgcatga agagggtgag
     2281 tgaaagtcct tgatccacgt gttcagtcgc agcagcgtaa acatcgatga ctttacgcat
     2341 atccatatcg taggcagaag tgtagtaagg aatggtttct gtagacaagc cagcagcagg
     2401 gtagtagatt ttaccaattt tcttttcctg acgttcttca atacgttgcg taatcgggtg
     2461 gatagaagca gaaacatcgt tgatgtagct gatagaacca tttggcgcta cagcaaggcg
     2521 attttggtgg taaagaccat cctcttgaac cttgtcgcga agttcagtcc aatcagcaac
     2581 accagggata aaaacatttt tgaagagttc tttaacacgg tctgatgttg gaacaaattc
     2641 accagttaca tacttgtcaa agtaacttcc gttagcatag tctgattttt caaagttgtg
     2701 gaaggtaata ccacgttcac gcgcaatatt gtttgactct accaaggtcc agtagttcat
     2761 aagcataaag tagatgcttg taaattcaac agactcaggt gaaccatatt caatgagttg
     2821 ttgggcaagg tagctgtgca gtcccatggc accgagacca aaggtgtggg cttggctatt
     2881 tccatggtca atcgttggta cagctacgat atgtgaacta tctgtaacga aagtaagggc
     2941 acgaaccata gcacggatag aacgaccaaa atcaggtgaa gtcatcatgt taaccacgtt
     3001 ggttgaaccc aggttacatg aaacatctgt tcccatttga aggaattctt gagcatcgtt
     3061 gatcaagctt ggttcttgaa cttgaagaat ctcagaacac aagttactca tgataatctt
     3121 tccatcgaca ggatttgcac ggttagccgt atcgatgttg actacataag gatagccaga
     3181 ctcttgttgc aatttagaga tttcagtttc caaatcccgc gccttgattt ttgtcttgcg
     3241 aatatttgga tttgcgacca attcatcgta tttttcagta atgtcgatgt aattgaatgg
     3301 cacaccgtat tctttttcta cagagtaagg gctgaagagg tacatttctt catttttacg
     3361 agccaattcg tagaatttat caggtactac aacaccaagt gatagagtct tgacacgtac
     3421 tttttcatca gcgttttctt tcttagttga aaggaaagcg atgatatctg ggtgaaagac
     3481 gttgaggtag acaacgccgg caccttgacg ttgccccaat tggttggagt aagagaagct
     3541 gtcttcaaaa agcttcataa caggaacgac acctgaagca gctccttcat agcctttgat
     3601 aggtgcacca gcttcacgaa ggttgctgag ggtaattccc acaccaccac caatacgtga
     3661 aagttgaaga gctgagttga tagaacgtcc gatagagttc atatcatccg tcacttggat
     3721 taggaaacaa gataccaact ccccacgacg agcacgtcca gcattcaaga aggaaggagt
     3781 agcaggttgg tagcgttggt ggatgatttc attggcaata tcgattgcaa cagcttcatt
     3841 cccatcagcg aaataaaggg cgttgaagaa gacacggtct tccatatttt caagatagta
     3901 ttcaccgtca ttagtcttta aggcatattg attgtaaaat ttataagctg ccatgaatga
     3961 cttgaattgg aagttttggt ctttgataaa ttgagccaat tcttccaaga actctggacg
     4021 gtatttcttg ataaaggctg tttcgatgta gttgtgttca atgaggtaat tgattttgtc
     4081 tttgattgaa tcaaaaacca tagtgtttgg aactacattt tctttaaaga aagcatccaa
     4141 ggcttcctca tctttatgaa gcatgatttg tccattaaca ggacggttaa tttcgttatt
     4201 aagacggaag taagtcacgt cttcaagatg ttttaatccc ataaaatttc ccttatctaa
     4261 ttacaaaaga aaggcttcta agttagccct aaaagcagtt tcttctggat gatgtactaa
     4321 gattatgcta attgtttcag ttttcctggt tggaaacctg aaaagacttc agttggtgtt
     4381 tggataacag gagctgcgct aaaaccgagc tctttaactt gatcgacgta ctcaggttgc
     4441 tcatcaagat tgatttcacg ataagagaca ttattactgt ccaagaaacg cttggtcatt
     4501 ttacattgga cacaattgtt tttagaataa acggttacca ttgtgtaact cctcttcaaa
     4561 atttaatact atcttagtat atcagaaaat aaaat
//
LOCUS       DAVMEI010000127         4594 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVMEI010000127 DAVMEI010000000
VERSION     DAVMEI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.127"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..170)
                     /locus_tag="VYQ24_001634"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="VYQ24_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(216..449)
                     /locus_tag="VYQ24_001635"
     CDS             complement(216..449)
                     /locus_tag="VYQ24_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1968751.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(455..1447)
                     /gene="plsX"
                     /locus_tag="VYQ24_001636"
     CDS             complement(455..1447)
                     /gene="plsX"
                     /locus_tag="VYQ24_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1968752.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1444..2214)
                     /gene="recO"
                     /locus_tag="VYQ24_001637"
     CDS             complement(1444..2214)
                     /gene="recO"
                     /locus_tag="VYQ24_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1968753.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2211..3380)
                     /locus_tag="VYQ24_001638"
     CDS             complement(2211..3380)
                     /locus_tag="VYQ24_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1968754.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3529..4539
                     /locus_tag="VYQ24_001639"
     CDS             3529..4539
                     /locus_tag="VYQ24_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1968755.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
BASE COUNT         1197 a         1182 c          874 g         1341 t
ORIGIN      
        1 ttctcaacct tgcttctctc cttagatagc gcatgattac aggctttatc ttcaactgtt
       61 agcggcttga gtttgctgga tttacgtgga gtttgtgctt gaggatatat cttcatgagc
      121 ccttgataac cactgtcagc caagattttt ccagcttgtc cgatatttct catttctaaa
      181 aaccatctac ttccgttgac ttggaactcc gattgctatt ttccttgaat gattttaacc
      241 acatctccta cgttttggag ttggtcaatt tcttcatcgc tgatttcgat actaaattca
      301 tcttccagag tcaagataaa ctccatcaag tcaacagaat ccgcatccaa atcgtctttc
      361 agactcaagg attctgtcac gacaaagtcc tctccctgtc gctcttggat aatggtcaca
      421 atacggtcaa aaatttcttt ttctgtcatc tcttttattc tcctgaaaat tcacgcgcag
      481 tctgggcaac tacgtctgtt tctagcatgg tacgaatctg gcgaatcgta ctataaacag
      541 ccttggcatc gcttgagcca tgagtcttga caacaggtgc cttaacacca aacaagaccg
      601 ctccaccaac atctgaataa ttgagctgtt ttttcaaacc actgaggctg tccttgagaa
      661 ggagggcacc tagtttcgct cgaagaccac cacctgtaat agctgtcttg agcaagccca
      721 tgattcccat agctgtccct tcgatggatt tgagcacagc gtttcccgtg aaaccatctg
      781 ccacaacaac atctgcaacg ccattcatca aatcacgcgc ttccacgttt ccgataaagt
      841 tcaaactttc atcagccgcc agtaattcat aagtttcctt acgaagcggg tcgcccttgc
      901 tactctctgt tccgttgttg agcaaaccaa cgcgtggttg cgcaatgcca cggacatttt
      961 tagcatagaa ggaacctaga accgcatatt gatggaggtg ctgggctgta ttttctgcat
     1021 tggcaccaag gtctagcatg tcaaaacctt ttccatcaac ggtaggcaag gtagacatga
     1081 gtccaggacg gtcgatattc ttgatacgac ccacgatgaa gaatccagca gccaacaagg
     1141 cacctgtatt cccagccgaa aggacagcgt ctgcttcacc atctttgaca gccttggctg
     1201 ccaataccat actggcattt ttcttattcc taatagctct cgtaggttca tcatccgaat
     1261 caatcttctc atccgtatgg ataatgctga cgcgctctgt cgctgtcaga tattgcttga
     1321 ttttagcttc atctccgtaa agttgaacct cgatatctga aaagtcagat agggcttgat
     1381 tgacaccctc aacaatggcc tgaggtgcgt aatcgccccc catggcatct actgcgattt
     1441 ttttcatttc ttttcctctt ttagtaattg tccccagtct gctagggaat caataaattt
     1501 ctttgatttt aggtgaatcc caacgtactc ttcatataat tgatccataa attggcgtag
     1561 ctcttgcttg attccaggct tgagcgaaat ggtctccaaa gtctcaaaat caatagcttg
     1621 aaattgattg agcagatagg ggatattggg attgagatga caacgtctct tatcctcatg
     1681 ataatgctct ggacagaggc aggctccata tttgaaagaa aagtcaaaag cctgaccaac
     1741 ccgatggcag aagacacact cattaaaatt gaggctgatt ccaaatcgag tcaaaatttg
     1801 aatttcaaaa atattggtca aaacctgata atccaagcct gcttccatca actccaaagt
     1861 cttttgcaaa aaagcaaaca agggagcatc ctgctgattg tcctgcaaac tagcatctgc
     1921 aagagctgcc acataggtcg cataggccat gacaaagagg tcactattaa tcttgggaaa
     1981 agtcatgacc tcatgatagt cttcgatgta actgagtccg tcatcattga ttcgcaatag
     2041 aaatcgtgcc agcaccaagg gctgaataac aggcgccagc ttagactgac cagcgtgttt
     2101 gacaaaaaac atgcgtttgc caacctgctc tgtaaaaatt ttgacgagct tgtcatcctc
     2161 acgaaaattg cgattgtaaa gcaccaagcc ttgactcgtg atagactgaa tcatgcttct
     2221 ctcatgtact cctcaagtcg tttcatggct tctttgatag tctccatgct ggctgcataa
     2281 gataggcgga cgtagccttc cccgtaacgt ccaaaggctg caccagggat aaaggcaacg
     2341 gccttcttct gagcaaaatc cttcagaaaa gcaaaggagt cttgattgta gcccgctgga
     2401 attttagcaa aaatatagaa ggcaccgtct ggtttgataa tctcaaaacc aagagcagtc
     2461 attttttcga tgatatagtc ccgacgctgg atatattcct tcttcatggg ctccgcatca
     2521 tttttaccag ccgtcaaggc ttctaccgca gcatgttgcg ccatggtatt tgcggcagtg
     2581 accaagtatt ggtgactctt gattaactgg gctgtgaagg ccgcaggagc gaaaatcagc
     2641 cccaaacgcc aacctgtcat ggcatgcgat ttagacaaac cattgataat aatagcctgg
     2701 tctctcaaca tcgttcctag agacacatgg gcttcgcctg tgtaggtcaa ttctgagtaa
     2761 acctcatcac agacaacaaa aatttcgtac ttgcgtaaaa cagctgccaa ggcctctaac
     2821 tgctctcgac tgtaggtaat tcctgtcgga ttggctggat agttgagaat aaccgccttg
     2881 agcttatcac cctgctccaa aatggccttc tccaacatct caggagtcaa gacaaaacca
     2941 ttttcagtcg tatcaatctc aacaatttct gccccaacta agttaacaat cggttcatag
     3001 cctggataag caggagctgg caaaagtacc ttgtcgccct cttccaaaat agccgtcaaa
     3061 gtcgcagata aagcctctgt cgccccaatt gtaaccaaga tttcattttc aggagcatag
     3121 tccagttggt acttttcctt aacaaagtca ctggctgcct gacgtagagt cagcagacca
     3181 ctcatccctg tatagtagga ttggttctga tcaatcgctc gcttggccgc ctccttgaca
     3241 tggtctggcg ttgtaaaatc aggttccccc aaggtcaaac gcaagacccc aggaatctcc
     3301 gaaatagcct ggtcaaactg acgaatcaac gaaacttgaa ttttatctaa ctgtttatta
     3361 aagcgcttag ttaagtccat agatacctcc tactttcaaa acgtatctct attatactac
     3421 aaaagccccc cgaccgcaaa aatacattag agaatagctt tccactttct attttacttt
     3481 tcctgtttac aggtctataa tggtaacaga ttctatttgg aggtttttat gtcatttcta
     3541 tcaaaaaatg gagcaggcat cttggtctgc cttctcattt ccatcctatc ttggtactta
     3601 ggaggattct tccctgtgat tggcgcaccc gtttttgcga tttttatggg catgctccta
     3661 catccctttc tctcgtctta taaacaactg gatgctggat tgacctttag ttccaagaaa
     3721 ttgctccagt atgccgttat cttacttggt tttggtctca atatctctca agtcttcgca
     3781 gttgggcaat cttcactccc tgtcatcctt tccaccattt caatagcctt gattgttgcc
     3841 tacctcttcc agcgcttctt tgccctggat acaaaactgg ctaccttgat tggagtaggt
     3901 tcttctatct gtgggggttc tgccattgca gcgacagcgc ctgttatcca tgctaaggaa
     3961 aaggaagtag cccaagccat ctccgttatc tttttcttca atgtcttggc tgcgctcatc
     4021 tttccaaccc tcggcacctg gcttcatcta tccaatgacg gcttcgccct ctttgcagga
     4081 actgcggtca atgatacttc ctctgtaacc gccacagcta gcgcttggga cagtctctac
     4141 cagaccaata ccctcgaatc tgcaaccatt gtcaaactca cgcgcaccct agctatcatc
     4201 cccatcactc tctttctctc ctactggcaa agtcgcgagc aaaaaaataa gcaaggctta
     4261 caactgaaaa aagtcttccc actttttatc ctttacttca tccttgcatc gctcctaacc
     4321 acccttctga cctctctcgg tgtgtctagt agtttcttta cccctctcaa acaactctct
     4381 aaattcctca ttatcatggc catgagtgcc atcggtctca aaactaatct ggtcgctatg
     4441 gtcaaatcca gtggaaaatc cattgttctc ggagccgtct gctggattgc catcatcctc
     4501 accagccttg gtatgcagac ccttatcggc attttctaat actcttcgaa aatctcttca
     4561 aaccacgtca acgtcgcctt gccgtatata tgtt
//
LOCUS       DAVMEI010000128         4577 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVMEI010000128 DAVMEI010000000
VERSION     DAVMEI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.128"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            101..334
                     /locus_tag="VYQ24_001640"
     CDS             101..334
                     /locus_tag="VYQ24_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1968756.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            334..1668
                     /locus_tag="VYQ24_001641"
     CDS             334..1668
                     /locus_tag="VYQ24_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1968757.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTIAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1678..1932
                     /locus_tag="VYQ24_001642"
     CDS             1678..1932
                     /locus_tag="VYQ24_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1968758.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            2028..2423
                     /locus_tag="VYQ24_001643"
     CDS             2028..2423
                     /locus_tag="VYQ24_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1968759.1"
                     /translation="MSKKLNRKKQLRNGLRRSGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            2835..3284
                     /locus_tag="VYQ24_001644"
     CDS             2835..3284
                     /locus_tag="VYQ24_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEV1968760.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3271..3828
                     /locus_tag="VYQ24_001645"
     CDS             3271..3828
                     /locus_tag="VYQ24_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1968761.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEE"
     gene            3830..>4577
                     /locus_tag="VYQ24_001646"
     CDS             3830..>4577
                     /locus_tag="VYQ24_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEV1968762.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDINFTYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDETVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALTELYGNYNKLFLK"
BASE COUNT         1408 a          815 c         1050 g         1304 t
ORIGIN      
        1 tatctacaac ctcaaaacac tgttttgagc aacctgcggc tagctttcta gtttgctctt
       61 tgattttcat tgagtattag aacatataat ggaggtcgtc atggacaata tcatcgatgt
      121 gtcaattcct gttgcagaag tggtggacaa gcatccagaa gtcttggaaa ttctagtgga
      181 gctgggtttt aaaccccttg ccaatccctt gatgcgcaat acagttggtc gtaaagtatc
      241 acttaaacaa ggttctaagc tagcaggaac tcctatggac aagattgtac gcacactgga
      301 agcgaatggc tacgaagtga ttggattaga ctaatggcag atgaacggat tcatgtccta
      361 cgggatattt tattagaatt gcataatggc gcctctcctg agtcggttca ggagcgtttt
      421 gatgcgacct ttgcgggtgt ctcagccatt gagatttccc ttatggagca cgagctgatg
      481 aactcagact caggtgtcac ctttgaagat gttatggaac tctgtgatgt ccatgccaat
      541 ctttttaaaa atgctatcaa aggtgtcgaa gtttcagata ctgagcatcc aggtcaccca
      601 gttcgtgtct tcaaagaaga aaatctggct ctccgtgcgg ccttgattcg cattcgtaga
      661 ttgttagata cctatgagtc tatggaagac gaggaaatgc tggcggagat gcgtaagggt
      721 ttggtgcgtc agatgggact tgtgggtcaa tttgacatcc attaccaacg taaggaagaa
      781 ctcttctttc ctatcatgga gcgctatgga cacgattcac ctcccaaagt tatgtgggga
      841 gtggatgatc agattaggga actctttcaa acagctctaa cgatagccaa gtcactacca
      901 gaagtgtcaa ttagcagtgt aaaggaagct tttgaagctt ttgcgacaga gtttgaaagt
      961 atgattttca aggaagagtc catcctcctc atgattctcc ttgagtcttt tactcaggat
     1021 gactggcttc agattgcgga ggagagcgat gcctatggct atgccatcat ccgtccgtca
     1081 gagaaatggg tgccagaacg acagagcttt attgaggaaa agattgcaga ggagcctgta
     1141 cagctagata cggcagaagg tcaagttcaa caagtcatag atacgccaga aggccatttt
     1201 accattacct ttacccctaa ggaaaaggaa gctgtgctgg accgccatag tcaacaggct
     1261 tttggtaatg gctatctttc agtcgagcag gccaatctca tcctcaatca cctccctatg
     1321 gagattacct ttgtcaataa agatgatatt ttccagtatt acaatgacaa tacgccagct
     1381 gatgagatga ttttcaaacg gacgccgtct caagtcgggc gcaatgtcga actctgccat
     1441 ccgcctaagt acttggacaa ggtcaaaact atcatgaagg ggcttcgtga ggggaccaag
     1501 gacaagtatg aaatgtggtt caagtctgag tcgcgaggta agtttgtcca catcacctat
     1561 gctgcagtac acgatgaaga cggagaattc caaggagtgt tggagtatgt tcaggatatc
     1621 cagccctacc gtgagattga tacggactat tttcgtggat tagaataagg agaaaaaatg
     1681 agttacgaac aagaatttat gaaggaattt gaagcttggg tcaataccca aatcatgatt
     1741 aacgacatgg cgcacaagga aagtcaaaaa gtttacgaag aagaccagga cgagcgtgcc
     1801 aaagatgcca tgattcgcta cgagagtcgc ttggatgctt atcagttctt gcttggtaag
     1861 tttgaaaact tcaaagcagg caagggattc catgatttgc cagaaggctt gtttggtgag
     1921 cgaaattatt aaacgagaaa gattcttgat ttttcactaa aatcttgata gaatgtttat
     1981 gttaaatcct tgtcagagca gggatttttt attgaaagga ttttatcatg tcaaagaaac
     2041 tcaatcgtaa aaaacaatta cgaaatggcc tccgtcgctc aggtgccttt tcaagtactg
     2101 tgactaaggt tgtagatgag acaaaaaaag tcgtgaagcg tgcagaacag tcagcaagcg
     2161 cagctggtaa ggctgtttct aaaaaagttg aacaagcagt agaagctacc aaagagcaag
     2221 ctcaaaaagt agctaattct gtagaagatt ttgcagcaaa cttgggtgga cttccacttg
     2281 atcgtgccaa gactttctat gatgaaggaa tcaagtctgc ttcagatttc aaaaactgga
     2341 ctgaaaaaga actccttgcc ttgaaaggaa tcggcccagc taccatcaag aaattgaaag
     2401 aaaatggcat caagttcaag taatttttct tgagccttgc atttccgaga aaaacttgct
     2461 acaatagagc cattagaggt gttttgaatc ccacatttta cagaaagtgg cggcgctgag
     2521 aagtccacaa atgtgtcaaa actggttgct aatgactgaa aaaatgaaat atttctgtct
     2581 ttttatttta aagactaaag atgcgggctt ggcccgaaat tgggtggtac cgcggataaa
     2641 cacattcgtc cctgtcatgt agatggcagg gcttttcttt tgtgtcttag tcaaaggagg
     2701 ttgttataaa acaatcattt aaaactaata aactatatta tggttttcct gtttttattt
     2761 taggatatca agattagaat tttggctata acatcacgac ctgtagttcc tcttatagtc
     2821 taggagattg ggttgtgatt ggagtcgttg cgagagagaa tgccgcagag cagatcaaac
     2881 agtatcaaaa atttactgtg aatatttctg atgaaacttc tatgcttgcg atggagcagg
     2941 ctggttttat cagtcatcag gagaaattgg aacgtttggg agtgcattat gaaatttctg
     3001 aacgaactca gattcctatt ttagacgcct gtccacttgt tttagattgt cgggtagata
     3061 ggattgttga ggaagacggt atttgccaca tctttgccaa gattcttgag cgacttgttg
     3121 ccccagaact cctggatgaa aagggacatt ttaaaaatca actgtttgcc ccaacctatt
     3181 tcatgggaga tggatatcag cgcgtttatc gctatctgga taagcgtgta gatatgaagg
     3241 gcagtttcat caaaaaagcg aggaagaagg atggcaagaa ctgagctgcc agataaaatc
     3301 gaaacagaac gtctcgtttt acgagtccgt actgtggcgg atgctgagga tatctttgac
     3361 tatgctagtt tgccagaggt cgcctatcca gcaggttttc ctccagtcaa gaccttggaa
     3421 gatgagattt attatctgga gcacattctt ccggagcgta atcaaaagga aaatctccca
     3481 gcaggctacg ggattgtcgt caaagtaacg gataagatcg ttggctctgt cgatttcaac
     3541 catcgccatg aagatgatgt gctggaaatc ggctatacct tacacccaga ctattggggg
     3601 cgaggttatg tgccagaagc tgcgcgtgcc ttgattgact tagcctttaa agatttgggt
     3661 cttcacaaga ttgaactaac ttgctttgga tataaccttc aaagtaaacg agtcgcggaa
     3721 aagcttggct ttaccctcga agctcgcata agagaccgaa aggatgttca aggaaaccgc
     3781 tgtgacagtc tgatatatgg cttgctgaag agcgagtggg aggaataaga tgagcgatgt
     3841 aaaagaggaa gtaagctctc tatctgagaa gcagttacgc caaattgatg ttgagtatgc
     3901 tgaattaaat gatagtgata ttatcgagcg attagcttat ttagaaatta acaacaatga
     3961 aaaacgtatt gtcatctctg atattgaacc aaccaaggaa ataatgtcag tctcggacca
     4021 aatttttgag attcaaaaga attttcaaaa aatcaagaat atgttcgaat tgtttatttc
     4081 agatgtctcg gattttttaa gtattaaaaa taaacttgag tcaaaggaat tagaaattga
     4141 agaggcagat gtaaatcgtt tcatgattca tttattatcc agtggaaaat tgtttgtcga
     4201 ttttaacgaa aaccaaatta aacaaaaata ttccaaagat agtgaggagt ttgattgtat
     4261 acatggtttt gcaagctatc agtatgatat caattttact tatcgatttt gccattcttt
     4321 gagaaattat tcgcaacata ctgacttacc aataaatgag gtaaaagctg ttagcccaga
     4381 tgatgagact gttataatag atttctatat tgatttagat tatcttttaa attccaactt
     4441 taaatggaaa aaattgaaag gggagttgat aaaactaaac caagaaacat ctaagattga
     4501 cgctatagct ctagtgaaag aatactttaa tgctcttaca gagctctatg gtaattataa
     4561 taagttgttt ttaaagt
//
LOCUS       DAVMEI010000129         4544 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVMEI010000129 DAVMEI010000000
VERSION     DAVMEI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.129"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(204..1679)
                     /locus_tag="VYQ24_001647"
     CDS             complement(204..1679)
                     /locus_tag="VYQ24_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1968763.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKATDSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(1937..2860)
                     /locus_tag="VYQ24_001648"
     CDS             complement(1937..2860)
                     /locus_tag="VYQ24_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1968764.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(2874..3803)
                     /locus_tag="VYQ24_001649"
     CDS             complement(2874..3803)
                     /locus_tag="VYQ24_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1968765.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(4122..4472)
                     /locus_tag="VYQ24_001650"
     CDS             complement(4122..4472)
                     /locus_tag="VYQ24_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968766.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRNILQTVKDAI
                     VSLESIKESQSATKDGGQTVGKETVDKDVLENSQEELQKAVLFLADEKGSEHTAAELI
                     DNLKEVIAKLKANA"
BASE COUNT         1379 a          864 c          897 g         1404 t
ORIGIN      
        1 tgagaaaacg aatgcaattt atttgactgg aaattgtaga ggattctcga aatttcctga
       61 atggttaaaa taaggacaag agaaaacagg gatatctata tccttgtgcc aaaaaaacca
      121 ctgccctccc cagatcaacc tgaggaaagc agtgattctt attttaggag ttaggaatga
      181 atacacgaaa tcaatttagc tgattatttt ttgtttttca agaattcatc gtattgtttt
      241 tgcatttcgt tcaatacttt ttcgtaggca ccttcagatt tcaatttttc catcaattct
      301 ggaatcgctt tatctgggtc tacagtacca gtgttgatag ctgtatcaaa ttgttgcatt
      361 gtgttagcaa tagctgagat ttcagatttc acattgtcag tattaaagat aaatccaagc
      421 gctggagatt ctttagcttc tgccaattct ttcttagaat tttcgatttg ttggtctgta
      481 acgttttcgt tgatgtaaag gatccagttg ttaccagtgt tccatccacc catgtgagtg
      541 tttcctttgt agccatcaag aacgcgaaca cggttttctt taccttcaat tttttcccag
      601 ttcttgcctt ctggaccgta aacaagaccg ttcaagagtt ctgggttcgt attcaagagg
      661 ttcaagattt ccattgattt ttctttgttc ttagagttgt ttgagatgac aaagttagca
      721 acttgtgttg tttggttttt cttgatgaag ttagtaattg gtttgatttg gatatctttg
      781 ttggcaacac gtgaaagcaa gctgttaccg tagtcagctg gtcctactgt ttcttcacga
      841 acgaaccaag tatcttgttg aaggtcaaag gaagtatcgc ttgttgcgac gtcttttgga
      901 atgtagccag cttcatagaa tttgtgaaga gtcttcaagt gttctttgaa acgaggtact
      961 tcgtaacggt ttacaacttt agtagtatcg ccttcaaggt caataacgaa tggaagaccg
     1021 tttgctactg ggtagtcaaa gttatcagat gggatgaaaa ctttaccaat agcaaatggt
     1081 actacgtctg gagctttttc tttgatttgt ttcaagactg gctcaagagt ttcgtaagaa
     1141 gtaacacctg aaatatcgat accatatttg gcaaggagag ttccgttgaa ggcaaagttt
     1201 tgagatgatg ctacgttagc tgcaactggt acagcataaa tcttaccatt tacagtatta
     1261 cccttgatat aagctggatc aagtgccttg taaaggtctt taccttcttt tttgtacaat
     1321 tctgtcaagt cagcataagc acctttttga gcatttacaa tatagttatc tgcaaaggcg
     1381 atatcatagt tttcaccaga tgatgtgata actgacattt tcttaccata gtcaccccag
     1441 ccaaggtatt ggatatccaa tttagcacca actttttctt cgatgatttt gttggcattt
     1501 gctaacaatt catccaagtt atctggtttg tcaccgattt ggtacatttt gataacaggt
     1561 ttgtcacctg aatcagtagc ttttttgctg ttacctgtca aatttccaca agcagcaaga
     1621 cctgcagcca aagcgactac actagcagat gcaaaagcat attttttcca gtttttcatg
     1681 atgaaaactc ctttttttat ttttaaactt ataaacaatg taatgatctt atactcaatg
     1741 aaaatcaaag agcaaactag aaaactagcc gcaggttgct caaaacactg ttttgaggtt
     1801 gtggataaga ctgacgaagt cagttacata tatctacggc aaggcgacgt tgacgcggtt
     1861 tgaatttgat tttcgaagag tattaacttc acacaaggga agttgggaac tgagaaatgt
     1921 tctttttcaa taagcactat tctttcacac caccgatagt caaacctttt acaaagtagc
     1981 gttggaagaa tggatataaa atcgcgattg gaagggttgc aaccacaacc atggccatac
     2041 gacctgtttc tttcggtaga gcaactccca gttgaccagt caagccgacc gctttggcaa
     2101 tgtagtccat attttgttgg atttgcatga gcaaatattg caatggatac aagttatcac
     2161 tcttgatgta aagaagggcg ttgaaccagt cattccagaa accaagagct gtcaaaagcg
     2221 tgatggttgc gatacctggt agtgacaatg gcaaacagat ttggaagaaa atccgggcct
     2281 cactggcacc atcgatacga gccgattcga gaatggcttc tggaatggtc ttcttgaaga
     2341 aggaacgcat caagatgata ttgaatggtg agagaagcat tggaacaatc aaggcccaaa
     2401 cagtgtcacc aagctgaagt acacgggtca ccatgatata acctggtacc aaaccagcgt
     2461 tgaacaacat actgagaagg acgaagatgg taaaaaatct gcgatactta aaggttgtcc
     2521 gtgaaatagc gtaggcatag gttgttgtga taaagacatt tgtcaatgtc ccaactacgg
     2581 ttacaaagac agagatgaag agggcttgta ggattttatc cttaaactgt gccaaaaact
     2641 caaaaccgtc taagccaaat tgggatggga agaagctata gccgtattgg aggaggcttt
     2701 tctcgtctgt cactgaaata atgataacga atacaaaagg taggatacaa gagagggcaa
     2761 tcaaacccga aatgatactg aagaagatat ctgctttctt actgaaggag tgaatgccga
     2821 cattatcaat tttttctttt ttaattttct tttttgccat attctcctcc tttctagaac
     2881 aaagctgagt ttggatcgac tcgtcttgca agcaagtttg ataggataac cagaatcaaa
     2941 ccaacaacgg attggtaaag accggctgct gcagccatac cgatatctgc tgtctgagtc
     3001 aaaccattaa aaacatatac gtccaaaacg ttggttacat tgtaaagctg accagcattg
     3061 tgtgggattt gatagaagag accgaagtct gcgcggaaga tatttccgac tgcaaggatg
     3121 gtcaatacag ttacaagcgg agtcaactga ggaatggtta cgttgcgaat acgttgccac
     3181 ttgctagctc cgtccactgt cgctgcttcg tagtaggttg gatcaattcc catgatcgtc
     3241 gcatagtaca tgacactgct atatccaaaa cctttccaaa tacctaggaa aagtaggaga
     3301 tagggccaga tgcccaggtc agcgtagaaa ttgacttctt tgagaccaag actttccaat
     3361 agatgattga acaccccttt atcaatattt aggaaggcat ctgtaaagaa actgatgata
     3421 acccaagaca agaagtaagg gaacaacata gaagtttgga aaatcttcac cattctctta
     3481 gaacggagct cgctgaggat aatggcaatc cctacagata caactaaacc tagaaagata
     3541 aagccaagat tgtagaggac agtatttcgt gtgataataa aggcgtctct tgaactaaat
     3601 aagaatctga aattatcgag tccgacccat ttactattta tgatactatc tatgaaacca
     3661 ttactggtca tgtggtagtc tttgaaggca accacgttcc caaatactgg aatgtaaaag
     3721 aatagaatca accagagtgc ccctggcaaa accatcaaga gaaagatcca gttgtctctc
     3781 aatgtttttg aaaacttttt cataatttcc tcccttttta ttttgatatc catctaaaaa
     3841 ttctttttta gacttttgat aacgattaca ttattagtat actcctattt gcaggttagg
     3901 ttaaactcct aattatagaa aaaactccac aaattatgta gcagatttaa aactttatca
     3961 ccactatcaa acaaatgtcc taaatcaatt gtttatttta tctctattag cccagtgatg
     4021 gcgtcactct gttataagca tccaacaacg gggtatactg aaaaatctcc agactaggga
     4081 actcagcgat agttcctaat ctggagattt ttaatatgtt attaggcgtt tgctttcaac
     4141 ttagcaataa cctctttaag attatcaatc aactctgctg cagtatgctc agagcctttt
     4201 tcatctgcca agaacaaaac tgctttttga agttcttctt gagagttttc aaggacatcc
     4261 ttatctactg tttctttgcc cactgtttgt cctccatcct tagttgctga ctggctttcc
     4321 ttaatggact ctagggaaac aatggcatct ttgactgttt gcaagatatt acgtaaacct
     4381 tgctctgtca aactatcatc tgcaaaagct ttcttagcct ctgccaaaac cagacgtgct
     4441 gaatctgtgg taggattcga tacacctgtc aatgatctca aaagattttc taaggtttga
     4501 gtctgcttac taatactaga ctaaaatcaa aaagtattat ataa
//
LOCUS       DAVMEI010000130         4504 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVMEI010000130 DAVMEI010000000
VERSION     DAVMEI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.130"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(142..1368)
                     /locus_tag="VYQ24_001651"
     CDS             complement(142..1368)
                     /locus_tag="VYQ24_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1968767.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1440..2105)
                     /locus_tag="VYQ24_001652"
     CDS             complement(1440..2105)
                     /locus_tag="VYQ24_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1968768.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2117..2794)
                     /locus_tag="VYQ24_001653"
     CDS             complement(2117..2794)
                     /locus_tag="VYQ24_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1968769.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            complement(2816..3049)
                     /locus_tag="VYQ24_001654"
     CDS             complement(2816..3049)
                     /locus_tag="VYQ24_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968770.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            complement(3335..4198)
                     /locus_tag="VYQ24_001655"
     CDS             complement(3335..4198)
                     /locus_tag="VYQ24_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1968771.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTALFE
                     LRLQQYKKLID"
BASE COUNT         1574 a          891 c          794 g         1245 t
ORIGIN      
        1 aaatgtttta gcaattgttt cgtactattt tagattcagt ctattataac acattcagaa
       61 aagagaaaaa agtctgttga ttttgaccat cataaaaaga ctggcaatcc agtctcaaac
      121 atatattata gaaattctcc actaaatact ttcacgaata ttcagaagca taacaaaggc
      181 aactagaaga aatagcaata aaacaaagct aactgccaga gttccaaagc tagtagcaat
      241 ggttaccaaa gctattgtaa ataagctagg taaaacaacc gtaatggcac cgatagagga
      301 ttgaactgct cccattgact cctcaggtat ttgtttaaag acgagttctt gcaatctagg
      361 agagagaaca cctgcgaaaa aggcatccaa ggtactaaag atgagaatcc agtcaaaacg
      421 aactgtggca aatcctacta gaagaagcaa ctggatgaca agtgaggcat agagagctgt
      481 ttttatggaa atggtatgtt gcagatagcc acttacaagg cttccgacaa tcagggctga
      541 cagttcaaaa gtggatagca aggcaagaga ttgacctgtt tgtaaattca aaaagggttg
      601 attcctcaaa aatagagtag agacaggaac tgtaacattt atcactgctt gactagtaga
      661 gataataaac aaaaccaaga gcaccttatt catattccat atcaatttcg atgattggag
      721 caaatgctgg caaaaggatt ttacagagag tccttcttga tagttaatcg ttttttctac
      781 tttcaagagg tcagttttta tgaagaggat acctaaaaat gcgattaaaa aggtaagagc
      841 gttcagcaag gaaatgaact ggatggagag aatccctagc aagactcctc ctagaatatt
      901 actgattgtt ttcactaaac taacagttga ctgtttaaat ccaatagctt ctgccagatg
      961 gtcttgccca ataattctaa tgaaaatcgg agtgagcatg gctcctgaaa agtaactcaa
     1021 tgtgtcagac aagaggttaa gcagacaaat aaatgctact agcaacaagg agaaagactg
     1081 ccctgaaagt gataaagaaa cgatagagta aagcaaaaat tttgcaaaac taatgactgt
     1141 gtatttcaag acacgatgat gttgaaaatc tgccaaaact cccagaaaga tttgtaaaat
     1201 ttggggaagg gtttctgaaa tcgtaatgag taaaatcgcc aaaggtgcaa aagacgcatc
     1261 tgccacataa ttcagaaagg ccagataaaa aatcgtatcc ccaagcgttg aaatccactg
     1321 gttgatagtt aattgcctaa aatctctatt ttgaagaaat actttcatca caactccttc
     1381 ttaagttcaa atgggaatct ttccccaagg atagaccgcg atactactaa caaccaaaat
     1441 tacagtaaca tcaaaagctg accaatgcca ttgtagacta tatgcagtcc aataggccaa
     1501 taaattgact ttgtcattct aaataagact gcaaatataa gacctccacc catatagaag
     1561 acaaagtctg tcaagaccca accgtgatta ctaatgtgcg agaccccaaa taaaacagcg
     1621 gaaccaagta catctagccc ccatttcttt cctttttcca gagcagtcat cactaatcca
     1681 cgataaatca tgtcttcaaa aatgggacct gcaatcacag gataaaaaaa atacatcaaa
     1741 aatgccgtag ctcctgtaaa agtaggagca gcatgttgat aagaaatttc atttcgagta
     1801 ggtgggaaaa gaaaaaaggt gacaaaattc caagcaacaa aagcaagcag agctaggaag
     1861 gaatagaaaa gataggatcc tttaaacttt ctactattga ttttctgcca tttccccgac
     1921 caaatcatag caataagagc aaataaaacc acaagaaaat tcaacatcat atccgacaga
     1981 taataggcaa agtcagatag cccagcaaca aggtcgctgc gtaaaactaa aacactgaac
     2041 ttctggtcag caataactag tagaaaaact ataataaagt agcggtgtga gattatcttt
     2101 ttcatatatc acctttctaa tatccaaata ccaataaagt aacaatgagt aagaaactat
     2161 tccatgaagc atgcagagct atagcccaat agatggatcg ggtgtagcga aacatcatac
     2221 aaaatatcaa gcccattcca aaatacttta tgaaatctgt cgttatccaa ccatactgca
     2281 aaacatgcat agcgccaaat atggcagcgg aaacaagaac atcaagatag tatctcttaa
     2341 ctttagataa acttgtcatc aaaagaccac gacaaacaac ctcttctgat acaggtgcga
     2401 taatactagt ataaagtatt cgcgtaacaa aatagctaat tcctgttaaa ttggtggcta
     2461 cttctacgac tgtacttcca ttctgggtac gaggaaagat ataggttgtt agatttgccc
     2521 acacgaacaa taagaaaaaa gaaagaagga aaacacccag gtaagaccaa cgaaactgga
     2581 aacgaccaca ctctttccaa tgttcacttt tgacaaaagc aattgtagct atagttccca
     2641 gaataagtac caataaaact tggaacacat agtacatatt atcagacaaa gcaaccataa
     2701 aatctaagtc tgatgtgaca ttaaaaatga ggtaataagt caaaatcaac aagccagttg
     2761 ctaggtgaaa tttcacttct ttcattttct tcatcctatt atctcctata agagcctatc
     2821 ttctacggcg gccaaacaat ccatctgcta aatctatagt ccaatcaaaa gctccacgat
     2881 taggactcat cccttgattg ccccaaccag ggtaaattcc tgggacgccc caaccagata
     2941 taccacttct tccaccacct cccatagaat ttacgaggtt gcctcctcta acatcttgca
     3001 actcagcttc tgtcaattcc attgtttctg caaattgtaa atttaacatc ttttacactc
     3061 cttcaattat cttcatttgt aaaccacttc tgcgacctag gatttgcttc aagtgcttta
     3121 caagtacagt ataacacgaa aattggctta ttttagaaaa tcgcatattt gatatttttt
     3181 cttatagaaa tttcagattt gcgattttgg tgaatttgat tacttctctg gtataataaa
     3241 gttactacta atgaggagtg gagaaatatg aagaaacaaa ttttaacatt attgaaaatc
     3301 gttgctgaga ttattattat tttgccattt ctaactaatc gataagcttt ttgtattgct
     3361 gcaaacgcaa ttcaaaaagg gctgtcaatt gtggattttc taatacttgc agagattgga
     3421 taaagcgttc aatctctttt tgattgcttc cctttgtttg aagaaagaca ctcatcttct
     3481 ttaaaaattg ccacgatact ttttcaaaaa catcatacgg tcgtaacatc ctctccaact
     3541 cggcttcgaa gattgggatg taggagaaaa gttttcgctc catgagttct gataagatat
     3601 ttaagagtcc ttgcttcata tacaatcgat tgtgtactaa ctctttaaat tctttggatt
     3661 tttcgagtaa ggaggttgat aaaaaaatca gatcttgatt gctcaagaag ggcatggtat
     3721 tgcaaaagag atagagttca aaccaggtcc aagactcgat agcatagaga taggtggtca
     3781 aaaactcgct atcctcctct gctagtgggt agcttttatt tagtgaatgg atggcatctt
     3841 taatcacgat ggcattcaaa cgacgatagg tctgcgccat ctgttcttga tcgacttcct
     3901 ccaatagctg ctctaaagca gctatatcct gatgggcaaa gcgattcaca acctttcgac
     3961 cgattcgcat atgtggagat tcttgatagt tgttgagctt gtgcccaaac tcatcaaagg
     4021 tcacatttat accttggata gctagaatca acttatccgc agacagcata gactgcccta
     4081 gttcaaactt ggacaactga gaagctgtta gaccctcaca agccacatct gtctgcttaa
     4141 gctttctcgc caaacgtaat tccttgtaaa attcccccag ttccattttt tcaatcatcc
     4201 taccacctcc tagcttttgc atattatatc attatttgtg ttcatttgtc aaaacattct
     4261 gacacttttg tgattgaaaa agccagcctt aagctgactc taaaaggacc gagccttgta
     4321 caatacagaa ctcaatcctc gttctaaata gaatgtctaa cttttttggg cagtacacga
     4381 gcttggggaa cagccactgg aacgatgagg tgtgagctca aaatatcctc cagttatgtt
     4441 tttcctaata gtataccgga agagtgaaag gattttataa tggagcggtt acaaagaacc
     4501 tact
//
LOCUS       DAVMEI010000131         4475 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVMEI010000131 DAVMEI010000000
VERSION     DAVMEI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.131"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            70..1332
                     /locus_tag="VYQ24_001656"
     CDS             70..1332
                     /locus_tag="VYQ24_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1968772.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            1335..2231
                     /locus_tag="VYQ24_001657"
     CDS             1335..2231
                     /locus_tag="VYQ24_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1968773.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            2246..2563
                     /locus_tag="VYQ24_001658"
     CDS             2246..2563
                     /locus_tag="VYQ24_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1968774.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLPGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            2580..2897
                     /locus_tag="VYQ24_001659"
     CDS             2580..2897
                     /locus_tag="VYQ24_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1968775.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            2907..4262
                     /locus_tag="VYQ24_001660"
     CDS             2907..4262
                     /locus_tag="VYQ24_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1968776.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
BASE COUNT         1318 a          630 c          993 g         1534 t
ORIGIN      
        1 aacttgacgt caaaaaataa agataattga taacttttga taataaaata tatttaagag
       61 gaaaaggtta tggaaaaatt attacaagaa aagttacttc cagtagcggc tagattgggt
      121 aataataaag cgttggtttc tattcgagac ggtattactc taactatacc tttattgttg
      181 ataggttcat tactaatggt tattgcaagt tttcctattc caggatggga aaaatatctg
      241 ggtgatattg gtgttgctga ctatttatgg aagggtgttg atagtagttt tggattactt
      301 ggtcttgtag ctagttttgg tatcgcttac tttatggcta gacaatacaa agttgatggt
      361 attcctgctg gtattgtgtc attatcatct tttattacag ttacgccttt cattagagga
      421 gaggctggag caggaatgcc taccgctttc atggcatcaa aaggtttatt tgttgctatg
      481 attttaggat tgattaatgg ctatatctac caatggttta taaatcataa tatacagata
      541 aaaatgccgg atggtgttcc accagcagta tctaaaagct ttagtgccat tattcctggt
      601 gcagtgacta ttgttggttg gttgattgtt tatgcaactt tagataaatt aagtttacct
      661 aatcttcatg aaattgctca agtagctttg ggaggtccac ttggactttt aggaaataat
      721 gttattggcc ttcttatctt aattttcctt aatagtagtt tttggtttgt aggattacac
      781 ggaggaaatg ttgttaatgc agttatgaaa ccgttatggt tagctaatct agatgcaaat
      841 aaagtagctt atcaaactgg agaaactctt ccaaatattt ttactagcgt atttatggat
      901 aattttgtat tcataggtgg cggtggtgca accataggtt tagttcttgc gttaggatat
      961 ttagcacata agaagaaagc tagtaaacaa ctaaaaactt tggcacctat aactgttata
     1021 cctggattat ttaatataaa tgaaccagca atgtttggcg ttcctattgt tttgaacatt
     1081 ttgttactag ttccatttat cttagctcca atgtttaact tactagtagc gtggggagca
     1141 atggcatcag gtttggttcc gctaacttat acagatccag gttggactat gccacctgtt
     1201 ataagtggtt tacttgctac aggaagtatt tctggttcat tattacaaat tgtattgatt
     1261 gttttggatg tcttacttta tttaccattt gtaatagcta ttgaaaaacg gtttaagtta
     1321 ttggaggatt gagaatgaaa cgtttaatta gtgcaaatcc atctgagata ttacagatga
     1381 atgctgaaga attaaaacaa agtattttag caagtgaagg tagagttgtt ctatctgaaa
     1441 atgtagttac tcgtgagaca tttgttgggg atataactaa ttctgaaatt gctagagctt
     1501 ttggagctga tatgatttta ttgaattgtg ttgatgtttt tgagcctaaa atttatgctt
     1561 tggatagttc aggtgatgat gttattcatc gcttacacca gcttgttgct tgtccaattg
     1621 gtgtaaattt ggaaccgatt gacccatctg caaagatgct agaggaaaca caggaaattg
     1681 ttgcaggtcg tgttgctagt gttgaaacat tgaatcgaat agaggagtta ggttttgact
     1741 ttgtctgttt gactggaaat cctggaacag gagttagcaa tcaagagatt atcaaagcag
     1801 ttcaatctgc taaagagaat ttctcaggtt tgattatagc tggtaaaatg cacggggcag
     1861 gagtgaatga gcctgtggca gagctttctg tcgcagagca attgttggaa gcaggtgcgg
     1921 atgtgatact tgttccagca gttggaaccg ttccagcctt tcatgaccaa gagttgcgtg
     1981 aagtcgttga tctcgttcat agtaagggtg gtcttgtatt gagtgctatt ggtactagcc
     2041 aagaaacatc tgatacagat actatcaagg aaattgcact tagaaataaa atttgtggag
     2101 ttgatattca acatataggt gacgcaggat atgggggact ggcaacagtc gataatattt
     2161 atgcattgag caaggcaatt agaggagtga gacatacagt atctcgcttg gctaggtcag
     2221 taaataggtg ataaaggagt aagctatggc taaagtaaca attatgttag catgtgcagc
     2281 aggtatgagt acaagtctgc tagtgacaaa gatgcaaaag gcagcagaag ataaggggtt
     2341 ggatgcagaa atttttgcag ttccagctcc tgaagcagaa gaaattgtag caacaaaaga
     2401 agtaaatgtg ttgcttttag gtcctcaagt tcgctattta ccaggggact ttcaagaaaa
     2461 actaaaagat agacagattc ctgtggcggt tattccgatg acagattacg gaatgatgaa
     2521 tggttctaaa gttttagatt tagctgaaag tttattagac taagattgag gagaatgtta
     2581 tggatgaaag taatttagaa tctgtaatgg ggctaattat gtatggtggg gaagccaaaa
     2641 gtaatgctat ggaggctatt caggcagcaa aaaaagggga tttctctaaa gccaatcgaa
     2701 gattagctga tgcgaatgct gccttattac aggcgcataa ggctcaaaca gaaatgttga
     2761 caagagaggc acagggggaa aaaacatcaa ttagcctctt gatggtacac gcgcaagatc
     2821 atttaatgac gagtctgact tttgttgact tggcgaaaga agtggtagaa gtatacgaac
     2881 gatttgaaaa aaattaggag tttggtatga atacaatgtt ggataaaatg caagaaaaac
     2941 tttctccaat tgcaatgaaa gttgggaatc agaagttttt agttgcttta cgtgattcat
     3001 ttgtggggac tatgcctgtt attatgacag gttccattgc tttgttatta aatgcttttc
     3061 tagtggattt accacaacaa tttcacctag aaagtattac gaaaaccttt cagtggcttg
     3121 ttgacatcaa taatttggta ttcaagggaa gtataccaat tgtttccttg ttgttcatct
     3181 attgcttggg agtgaatatc gctaagattt acaaggttga tacagtttct gctggtttgg
     3241 tatcacttgc ttcgtttgtc atttcgattg gaagtacagt tacaaagagt ttccctttgg
     3301 caaatgtagg agatgtgaaa ttagatcaaa tcttacaagg aattgataat ctagcatttg
     3361 atggtaaaaa tctgatggta actattggga atgtgattcc aggaaaccat attaatgcca
     3421 gaggctactt tacagccatg atgattggat ttttagcctc tattattttc tgtaaagtaa
     3481 tgaaaaagaa ctgggtaatc aagttaccag attcagttcc ccctgcgatt gctaagccgt
     3541 tcacttctat cattccagga tttatggcga tgtatatagt agccatcttg acttatgttt
     3601 tccatttact tagcaatgac ttattgattg attgggttta caaggtgtta caaacgccat
     3661 tactaggttt gtctcaaagt ttctttgcag ttattctgat gatctttttg aataagttat
     3721 tctggttttt tggtctccat ggaggaaatg tattggctcc catcatggaa gggctgtttg
     3781 gagttgctat gttagcgaat ctggatgctt tccaaaaagg tgaaccgatt ccttatatat
     3841 ggacaagtgg gtcttttggg gcgtttgtct ggtttggagg attaggatta gtacttgcta
     3901 ttttaatttt ttcaagaaac agtcattatc gaaaagttgc taaacttggt ctagcaccag
     3961 ttctgtttaa tattggtgag ccggtcaatt atggtttacc agttgtcttg aatcctttgc
     4021 tatttatacc atttgtactt agtccggttt tcatggcaac ggtagcttac tgggcaacaa
     4081 gctggggtct tgtttcacct gttacacaaa acgttacttg ggtaatgcct ccaattttat
     4141 atggtttctt ctctacagca tttgattggc gtgccatcat cttatcagtt gtttgtttga
     4201 ttattagtgt cttgacttat ttcccatttg tgaaaatggc atataaaact gaattgagtt
     4261 aatggtctta cttgcgtttt ttgtgataaa atagaaatag attacgtgta agatgagtta
     4321 ggtgtaggat ttcttatctc cactaaaatt gttattagac aaaggtttat agtttattga
     4381 agttggaata gtacactgta acttctaaaa cagtactaga aaatgatttg aatttcctaa
     4441 tcgatttagt catattttag ttcatttttc tataa
//
LOCUS       DAVMEI010000132         4352 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVMEI010000132 DAVMEI010000000
VERSION     DAVMEI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.132"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(26..199)
                     /locus_tag="VYQ24_001661"
     CDS             complement(26..199)
                     /locus_tag="VYQ24_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1968777.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(358..1965)
                     /locus_tag="VYQ24_001662"
     CDS             complement(358..1965)
                     /locus_tag="VYQ24_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1968778.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAHEALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2118..3602
                     /locus_tag="VYQ24_001663"
     CDS             2118..3602
                     /locus_tag="VYQ24_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1968779.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVSEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3697..4179
                     /locus_tag="VYQ24_001664"
     CDS             3697..4179
                     /locus_tag="VYQ24_001664"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1968780.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWARLILEQGISDDAFERHVI
                     "
BASE COUNT         1263 a          996 c          830 g         1263 t
ORIGIN      
        1 gaggctcttt tgtcttgttc ttgtttcaat tgactatatt atcagcgtaa tagtctaata
       61 gatagttaaa tgtagttgga tttccactaa aggttgatga gtaagttttt gtatctgaac
      121 ctgattggcc gcaagcagct aaaagcaaag cagatgcaaa agtcaggcct gcactaagga
      181 cacgcttctt tatgttcatc ttctttctcc ttaatagtgg gaatttgtaa agttaattga
      241 atttcgagaa tgaaagttta taaaccttgg ttataaaaaa caaaggattt ctgcttttta
      301 tacagtcctc ccttgttttt atatgatttc aattttaaat ttttctgcaa aaaatattta
      361 tagtaattcc acacagaaag catcccatgg aactaagatt tgtttttcaa agacttcttg
      421 agctagggtg ttttcaatca agacagattt gacttttcct tctactgtca agtcttgctc
      481 ttcattggac aagttagcca caactaggaa gcgacggtcg ccatccttac gtatataagc
      541 aaagacctta tcagccgtat caagcaattc aaagtcagct cgaattagcc agctattctc
      601 cttgcgaatt tggaccagtt tctgataggt atagaaaata gaatctggat ttgccagcgc
      661 ttcttggaca ttgatcatct cgtaatttgg attaaccgcc aaccaaggtt gacctgttga
      721 gaaaccagcg tttttgctct cgtcccattg cataggggta cgagcattgt cacgtccaat
      781 aacacggata ctgtccatga tttcttcaat cggaacacct ttttcaagag cctcatgcgc
      841 atagttgaga gattcaatat cttctacttg atccagtgtt tcaaacggat agttggtcat
      901 cccaatctcc tcaccttggt agatataagg agttcctctc ataagatgaa gcaagattgc
      961 aaaggctttg gcagattttt cgcggtattc ttggtcattt ccccagattg agacgatacg
     1021 agggaggtca tgattgttcc agaagaggga attccaaccg tcctcaactc ctaactctgt
     1081 ctgccatttg ttgaagattt cttttaactt agcgatattc agctcttttt gatagtgcca
     1141 tttaggctga ccttcctgat actgaagacc gatatgttca aactggaaga ccatagacaa
     1201 ttcttgcccc tttggatcag agtagagctt ggcaatctct ggcgttgctc cccaagtctc
     1261 ccctactgtc aagagatcct tatctccaaa ggtcgcctga ttcatttcct tgagataggg
     1321 atggagcata ggaccattat tgactacctt ctcgtcagga attttgccaa tcatgtcaat
     1381 aacatccata cggaaaccac caataccttt atcaatccag aagttcatca tctcataaat
     1441 tttctggcga agtttttcat tttcccagtt gagatccggc tgttttttgc tgaaaaagtg
     1501 gagatagtat tgacctgact tttcatcgta ttcccaagca gacccactaa agatagaatc
     1561 taggtcattg ggttcatcgc gccagatata gtagtctcgc tcagggctgt cagtattttc
     1621 acaggcttcg acaaaccaag catgttcatc tgaggtatga ttaaccacca agtccatgat
     1681 gatacgaatg tcacgcttct tagcttctgc aatcagctga tccatgtcct ccatggttcc
     1741 aaaaatagcc gcaatcgctt gataatcagc aatatcgtag ccattatcat ccatagggct
     1801 gtcataaacg ggagaaagcc aaatcgctgt gattcctaac ttagctagat agtccaactt
     1861 actggtaata cctggcaaat caccaactcc atctccatta ctatccataa aactctttgg
     1921 atagacttga tagactacgg cattatgcca ccatttttct tgcatcttct tacctcatta
     1981 ttttcataat ctatagtcta tgatagcact ttttaaaatt ttgtgcaagc gtttgcctaa
     2041 tctttttgat tcctttttta actccatagt ttcctaactt ccaatttttt attataatag
     2101 aggtcagagg taaaaaaatg aaaaaacaag cttttagttc tgaacaatat ttgaatttac
     2161 aacgcgacca tattttggag cgcattaacc aatttgacgg caagctctac ttagagtttg
     2221 gcggtaaaat gttagaagat ttccacgctg ctcgtgtcct tcctggttat gaacctgaca
     2281 acaaaatcaa gctcttgcaa gaattgaaag agcaggttga ggttgtgatt gccattaatg
     2341 ctagcaacat tgaacattcc aaagcacgtg gcgacttagg catttcttat gaccaagaag
     2401 ttcttcgttt gattgacaaa ttcaatgaat tagggatttt tgttggttcc gttgtcatta
     2461 cacaatacgc tggccaaccc gctgcagatg ccttccgcaa ccaacttgag aaaaacggaa
     2521 ttgattctta tcttcattat ccaatcaaag gatatccgac ggatatggat cacatcattt
     2581 ccccagaagg catgggcaaa aacgactaca tcaaaaccag tcgcaacttg attgtcgtaa
     2641 ccgctcctgg acccggttct ggaaaattgg caacgtgtat gtccaatatg taccacgacc
     2701 aaatcaatgg catcaaatct ggctacgcta aatttgaaac cttccctgtt tggaatcttc
     2761 cccttcatca tccagttaac ttggcttatg aagctgcaac agctgacctt gatgatgtca
     2821 acatgattga ccccttccat cttcaaacct atggagaaac cactgtcaac tacaaccgtg
     2881 atatcgaaat cttcccagtg ctcaagcgca tgttggaacg tattctcgga aaatcaccat
     2941 acgcttcacc gacagatatg ggtgtcaaca tggttggttt cgctattaca gatgacgaag
     3001 ctgctgtcga agcttctaaa caagaaatca tccgccgtta ctatcaaaca gttcttgact
     3061 tcaaagctga aaaagttagc gaagctgccg tcaagaaaat tgagttgctc atgaacgacc
     3121 tcggtatcac acctgcagac cgtaaggttg ctgtcgttgc gcgccaaaaa gcagaagaaa
     3181 ctggtggacc agccctagcc tttgaattgc caaatgggga aatcgtcact ggtaagaact
     3241 cagaactctt tgggcctaca gccgctgcct tgatcaacgc catcaaaaaa tcagctgaca
     3301 tcgctaaaga agtaaaacta atcgagcctg aagttgttaa gccaatccaa ggtcttaaaa
     3361 tcgatcatct cggtagccgc aatccacgcc ttcattcaaa tgaaatcctg attgcacttg
     3421 ctatcacagc tacagaaaat cctgatgctg cccgcgctat ggaagaactc ggcaacctca
     3481 aaggaagcga agcccactca accatcatct tgactgatga agacaagaat gtccttcgta
     3541 aactgggtat caacgtaacc tttgacccat actaccaata cgaccgctta tatcgtaagt
     3601 aaagatttca acctctccag cctcggagag gttttctctc tgtctcagga gcaaccttta
     3661 tgatataatg agagaagaaa actgaaagga tttaccatgt caaaagaagt tattgtcgaa
     3721 agttttgaac ttgaccacac cattgtcaaa gcaccctatg ttcgcttgat tggggaagaa
     3781 acaggaccaa aaggagacat catctccaat tatgatattc gcttggtgca accaaacgaa
     3841 gactctatcc ctactgccgg ccttcacact atcgagcacc tcttagccaa actcatccgt
     3901 acccgcattg acggcatgat tgactgttca ccatttggtt gtcgcacagg cttccacatg
     3961 attatgtggg gacgtcacac cagtgctaaa atcgcagctg ttatcaagga ttcgctcaag
     4021 gaaatcgctg aaactactac ttgggaagat gtcccaggga caaccatcga atcttgcgga
     4081 aactacaagg accacagcct cttctctgct aaagaatggg caagactcat cttggaacaa
     4141 gggatttcag atgatgcctt tgaacgtcat gtgatttaaa atcgagtagg ggataatctc
     4201 tagcccctct cacaccaccg tacgtgccgt tcggcatacg gcggttcaac taacttttaa
     4261 cgcatgtcgt tcaaggtaat aatccaaaca cgaaaccagt ccacgttttt caaggactgg
     4321 ttttgatata gcacgtttaa gtaccgactt ct
//
LOCUS       DAVMEI010000133         4209 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVMEI010000133 DAVMEI010000000
VERSION     DAVMEI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.133"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            213..986
                     /locus_tag="VYQ24_001665"
     CDS             213..986
                     /locus_tag="VYQ24_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1968781.1"
                     /translation="MKKMTASTMKVSTWNINQRSGQGKQIPEMIAQELAKQNSEIICL
                     TEYVKKTDNHSQFCSILQTNGYTIFEDVRTIDFGNEILIAIKSDLVNDCQVITIDNDD
                     NNPNFLQVRVTIEGQELNIIGVRIKIGGENIFEDFKNRKSQLDKLIFNLPTHENTIVL
                     GDFNNGYYKQNDDTHSYQGKPREFYSYPLLQSEMNKAGLTVHTPSKENSWKYCKLDHI
                     IGNIPISNVCYSWRFLENPDYQNKVGYPDHAILSATISL"
     gene            1028..1246
                     /locus_tag="VYQ24_001666"
     CDS             1028..1246
                     /locus_tag="VYQ24_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968782.1"
                     /translation="MSDYLKAYLSLLGFVILASIGIFILPQGHFFSVSALVLGCIPLL
                     ISTYYFAKIKLIGVYEFLKDIMDKIKGI"
     gene            1248..2567
                     /locus_tag="VYQ24_001667"
     CDS             1248..2567
                     /locus_tag="VYQ24_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="otitis media-associated H10"
                     /protein_id="HEV1968783.1"
                     /translation="MFKLRTVADLTRNYIISSSKLKVAHWSILVLSICTLLTIIANIR
                     APDLIKIPLFSLAGFVTYKFHRKLEYFRYNLDYYLIIRESLLYVLYTNKLYTIYKDNT
                     GHEKISRSAVLQYELDKQKGHIFIKAIITGDDFTTRVQSLDDVLAGALGIELEEKIIR
                     PSYVEYHFYYKKPDRLVLQSHNQRQEIDSLEIDLGYGVNYNPIKCPHILVSGGTGSGK
                     SVFISFLILELLKRQSTVYIADPKTSDLGSLSYYFGEKYVATTPNNIARIVRLVVEEM
                     QSRYQYMRDNFQYGSNFTDHGFKPVWLIFDEMGAFQASGTDKKSKEIITEVMDGIKQI
                     ILLGRQSGIFILVSAQQMRSETLNTDLRDNLQLRISLGENSSEGRRMVFGSATPDKFK
                     PIEVKGAGYLYMQGSGKETAQYWESPYLDTTQFDFIKELQLYLTETN"
     gene            2657..2971
                     /locus_tag="VYQ24_001668"
     CDS             2657..2971
                     /locus_tag="VYQ24_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968784.1"
                     /translation="MKLNDFLKPELLGNRFFAVKGYTEVLDRETNKPTALRLNVSIQD
                     ESSDFFMEMIQVKVNTLTPTASVQEMVNNKTCPVSLKDLNVGQYNGNLWFTCSDVILI
                     TK"
     gene            3009..3398
                     /locus_tag="VYQ24_001669"
     CDS             3009..3398
                     /locus_tag="VYQ24_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968785.1"
                     /translation="MQIFTYNDFLKEKEIVTFEQAEIILDELIKSSNIYDPEFQAYWK
                     ELIEYSAKYAEMRGKWRILTKEEQDTLDETRTNIHNRIRDNLISIRGLAQINNKDASW
                     FDKFHNDRQRMGDFANYINYIYAVNSR"
     gene            3417..4187
                     /locus_tag="VYQ24_001670"
     CDS             3417..4187
                     /locus_tag="VYQ24_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1968786.1"
                     /translation="MAIYYSVSDIHGHYNEFIKSLNKIDLSDKQSKLILLGDYIDYGS
                     QSFQVISKIIELEKIYPKQIITLFGNHEEWFYDWLILDNPTASAFPETIKSFFSTEEL
                     NYILKSNPNNFETGVRNEIRNNDKYIPFINWFQKRYEDSRYFETENQIFVHAGIDEEA
                     NNLWKDLTSPEIFTNKFPITTGRFYKDIISGHFASWEVAKDKSYLGKIYHDNKSHYFI
                     DGDVKNSKIIPVICYDTVKKSYQYSKNTSLLPKKFDRA"
BASE COUNT         1526 a          661 c          694 g         1328 t
ORIGIN      
        1 agaggaatat cccacaaata tactatgtaa aaggaggggg ctagtatgga cgaattggaa
       61 caagaagctt tagattactt ctatgaagaa ttggaaagct attatgatga tgaagacgaa
      121 caaggagaaa tctttgacgg cttgaacgac tagcaggagg agttcttctc ctcttgctga
      181 tttactaata gaatagaaag gtttatttat atatgaaaaa aatgacagca tccacaatga
      241 aagtatccac ttggaacatc aatcaacgtt caggacaagg aaaacaaatt cctgaaatga
      301 ttgcacagga attagctaaa caaaattcag aaattatatg tctgacagag tatgttaaaa
      361 aaacagataa tcattctcaa ttttgttcta tcctacaaac taatggttat acaatctttg
      421 aagatgtgcg tactattgat tttggaaatg aaattcttat tgcaataaag agtgatttag
      481 ttaatgattg ccaagtcatt actatcgata atgatgataa taacccaaat tttctacaag
      541 tcagagtaac tattgaagga caagaattaa acattattgg agttcgtatc aagataggag
      601 gggaaaatat ttttgaagat ttcaaaaacc gcaaatcaca actcgacaag ctaatcttca
      661 atttacctac acatgaaaat acaattgttc taggtgactt taacaatggc tattacaaac
      721 agaatgacga tactcattcc tatcaaggca agcctagaga gttttactct tatccattac
      781 ttcagtctga aatgaataaa gcaggtttaa ctgtacatac cccgtctaag gagaactctt
      841 ggaaatattg taaattagac catattatag gaaacattcc tatttctaat gtgtgctatt
      901 cttggagatt cttagaaaat cctgattatc aaaataaagt cgggtatccc gaccatgcta
      961 ttttaagcgc gactatatct ttatagtttt ccttaaaata attgctcatt aaaggaggtg
     1021 atgtaaaatg agtgattact taaaagcata tctatctctt ttaggatttg tgatactagc
     1081 tagcatagga atttttattc ttcctcaagg gcatttcttt tcagtttctg cccttgttct
     1141 agggtgtatt ccacttctta tttctactta ttatttcgca aaaattaagc ttataggagt
     1201 ttacgaattt ctaaaggaca tcatggataa aataaaaggt atctaacatg tttaaattac
     1261 ggacagtagc agatttaaca agaaattata tcatttcaag tagcaaatta aaggtggctc
     1321 attggtcaat actggtactt tctatctgta ctctgcttac tataattgca aatattagag
     1381 ctcctgacct tattaaaatc cctttgttta gtctagcagg atttgtcaca tacaaatttc
     1441 atcgtaaatt agaatatttt agatataatt tggactatta cctaattatc agagagagct
     1501 tactttatgt actatataca aacaagctct acaccatcta taaagataat actgggcacg
     1561 agaaaatctc aagaagtgcg gtcttacaat acgaactaga taaacaaaaa ggacatattt
     1621 ttatcaaagc aattataaca ggtgatgatt ttaccacaag agtccaatct ctcgatgatg
     1681 tgcttgctgg agctcttgga atagaactag aagaaaaaat tatccgacct agctatgtag
     1741 aataccattt ttattataag aaacctgata gattggttct acaatctcac aaccaaagac
     1801 aagagataga tagccttgag atagatttag gatatggtgt aaattataac cctatcaaat
     1861 gcccgcatat tttagtatca ggaggaactg gaagtggaaa atctgtcttc atatctttct
     1921 tgattttaga acttctcaaa cgtcaatcaa cggtatatat agcagaccca aaaacttctg
     1981 acctaggcag cttatcgtat tactttggag aaaaatatgt agcaaccact ccaaataata
     2041 tagctcgtat tgttcgtcta gtagttgaag aaatgcaaag tcgataccaa tatatgcgtg
     2101 ataactttca atacggctca aattttactg accacggctt taaaccagtc tggttaatct
     2161 ttgatgaaat gggagccttc caagcttcag gcacagataa aaaatccaaa gaaattatca
     2221 cagaagttat ggacggtatt aaacaaatta ttctactcgg aagacaatct ggcatattta
     2281 tccttgtatc cgctcaacaa atgcggtctg aaacgctgaa tacagacctc agggataacc
     2341 tacagctacg gatttcatta ggtgaaaatt caagtgaagg taggcgcatg gtctttggct
     2401 cagctactcc tgataaattt aaacctatcg aggtaaaagg agcaggatat ctttatatgc
     2461 agggttctgg taaagaaact gcccaatact gggaaagtcc ttaccttgac accactcaat
     2521 ttgactttat caaagaatta caactttatt taaccgaaac aaactaattt caacggatgt
     2581 tgttgcttaa attcgataga ttttcagcaa caatatctgt tcctcatcgc tctgcgatga
     2641 tagaaaagag gtacacatga aactaaatga ttttttgaaa ccagaattac taggcaatcg
     2701 tttctttgct gtaaagggct atacagaagt tttggataga gaaactaata aaccaactgc
     2761 cttacgacta aatgtatcaa tccaagatga atctagtgat ttctttatgg agatgattca
     2821 ggtaaaggta aatactttaa ctcctactgc ctctgttcaa gagatggtga ataataaaac
     2881 ttgtccagtt agtcttaaag atttaaacgt aggtcaatat aatgggaact tatggtttac
     2941 ttgttctgat gtaattctaa ttactaaata atgtgttata attatttcaa aaattaaagg
     3001 agcttactat gcaaattttt acttataacg attttctaaa agaaaaagaa atcgtaactt
     3061 ttgaacaagc tgaaataatt ttagatgaac ttataaaatc atcaaacatt tatgaccctg
     3121 aatttcaagc atactggaaa gaacttatcg agtacagtgc gaagtatgct gaaatgagag
     3181 gaaaatggcg aatacttaca aaagaagagc aagatacact tgatgagaca agaactaata
     3241 ttcataatag aattagagat aaccttatat ctattagagg cttggctcaa atcaataaca
     3301 aagatgcctc ttggttcgat aagtttcata acgatagaca acgtatgggt gattttgcta
     3361 attatataaa ttacatatat gctgtaaatt cacgttgatt atagaaagaa tcttatatgg
     3421 caatttatta ttccgtatct gatatacatg ggcactacaa tgagtttatt aaatctctca
     3481 ataaaataga tttatctgat aaacaaagta aacttatctt acttggagac tatattgatt
     3541 acggttctca atcttttcaa gtcatttcta aaataattga acttgaaaag atttatccaa
     3601 aacaaattat tacacttttt ggaaatcatg aagaatggtt ttatgattgg ttgattttag
     3661 ataatcctac tgcttctgcg tttccagaaa caataaagag tttcttctct acagaggaat
     3721 tgaactatat tttaaaatca aatcctaaca actttgaaac aggtgttaga aacgaaatta
     3781 gaaataatga taagtatatt ccatttataa actggttcca aaaacgatat gaagatagtc
     3841 gttattttga aacagaaaat caaatttttg tccatgctgg cattgatgaa gaagctaata
     3901 atctttggaa agacctaacg agtccagaga tatttacaaa caaatttcct attacaactg
     3961 gtagatttta taaagatatt atctctgggc attttgcttc ttgggaagta gctaaagata
     4021 aaagttacct aggtaagatt taccatgata ataaaagtca ttactttatt gatggagatg
     4081 taaaaaatag caaaataata cctgttatct gctatgatac agttaaaaaa tcctatcaat
     4141 attcaaaaaa cacttctcta ttgcctaaaa aatttgatag agcttaattc tattcttaga
     4201 ctaatatca
//
LOCUS       DAVMEI010000134         4136 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVMEI010000134 DAVMEI010000000
VERSION     DAVMEI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.134"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            720..1712
                     /locus_tag="VYQ24_001671"
     CDS             720..1712
                     /locus_tag="VYQ24_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1968787.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQKIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VYQ24_001672"
     CDS             1714..2532
                     /locus_tag="VYQ24_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1968788.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VYQ24_001673"
     CDS             2534..3319
                     /locus_tag="VYQ24_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1968789.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3417..3635
                     /locus_tag="VYQ24_001674"
     CDS             3417..3635
                     /locus_tag="VYQ24_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1968790.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3620..3781
                     /locus_tag="VYQ24_001675"
     CDS             3620..3781
                     /locus_tag="VYQ24_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1968791.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNP"
BASE COUNT         1153 a          703 c          896 g         1384 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gaatgataaa attaatcaat taatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta caaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cggtgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tatttcatga
      541 gagaaatcct aatttcacaa tctataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttctgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aacattttgt gaagattgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccaggaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgtatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg agcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaatca agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca aaagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctatc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttcg tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttaatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagga acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaggc agttgtcgtt gaggttttga
     3421 aggatttgcc agaatggttt ggaatcccag aaagcacaca agcctatata gaaggaacca
     3481 cgacactgca agtttggacc gcctatcagg agagtgattt gactggattt gtaaggttat
     3541 cctattcgag tgaagattgt gcagagattg attgtctcgg cgtaaaaaag cttatcaagg
     3601 tagaaaaatt gggagccaat tgcttgctac tttagagagt gaagctcgta aaaaagttgg
     3661 ttatctgcag gtcaaaacag tggcagaagg ttctaataaa gattatgatc gaacaaatga
     3721 cttttatcga ggtcttggct ttaaaaagtt agagattttt tctcagctat ggaatccgta
     3781 aaatccttgt cagattttga ttaaaaagct tgaataatat tacttgacat ctattctcag
     3841 agtgccatac tgtaagtgta atcgccgatt tagctcagtt ggtagagcaa ggcactcgta
     3901 aagcctaggt tataggtaga taaacgactg aggagttgaa aaaatagata ggtagaagat
     3961 aaccgttaag ccttattctt agcggttatt tatattgttt aatagcgctg atatttcatc
     4021 aattatgcct gttttcgtgt ttctggtagt tgttcaagtt tattgctact atttttgatg
     4081 gtatgaatgt gcttataatg tatcccggtt aacgaaagtt ttggacttat actcat
//
LOCUS       DAVMEI010000135         4075 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVMEI010000135 DAVMEI010000000
VERSION     DAVMEI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.135"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..753
                     /locus_tag="VYQ24_001676"
     CDS             1..753
                     /locus_tag="VYQ24_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1968792.1"
                     /translation="MGDLHLKSQLILPIVDKIIQTHNIKRIIFLGDYVDLHGQTNNIQ
                     LYAKDLTFLYSWKIDKEQNGIDVINLIGNHDAYYLLGEQAPFSIQNLEVFFAVKELLQ
                     NLKLQIAYQLDDYLVSHAGFNLIFDPKEWYFNLYTKEHEEELDILAYTIGPMRGGKAL
                     GGSPLWAHFRELELLPNQDYPKQIVGHTPKESIDISKNVIGIDTFSLYIDKDNKYQFI
                     GNGDLLVYDQGHFEVISTDWATDKILKQLPRL"
     gene            943..1731
                     /locus_tag="VYQ24_001677"
     CDS             943..1731
                     /locus_tag="VYQ24_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEV1968793.1"
                     /translation="MTSYNKKIVRTSSYIEIWEYEKPIFSKGKSILDTNTDNKEKSKR
                     RTFDELTPEEQEQRLERMKKTRLEAKWNLLRLVDCNFDDTTSFLTLTTKENISDRNHF
                     KDLLKTFIKRFNYHIFQTKKSKLKYIAVLEKQKRGAWHAHILLFSVPYVPHRQLLELW
                     GHGAVRINKVDVDSKENRGRYVTKYFEKGIGQELLENFGKQAYLSSRNLKKPDEDKFY
                     TYETFDYDSSTVLYETEYTSKVYKDGQYFDNRVTYKKIKLDKKE"
     gene            1737..1943
                     /locus_tag="VYQ24_001678"
     CDS             1737..1943
                     /locus_tag="VYQ24_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEV1968794.1"
                     /translation="MIATVNKNDLVALGFSEGTSKRIIRQGKELLVARGFRVYQNKRI
                     GTIPATIATELLGFDVQNTPLIRG"
     gene            1947..3080
                     /locus_tag="VYQ24_001679"
     CDS             1947..3080
                     /locus_tag="VYQ24_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1968795.1"
                     /translation="MAIRKDKDNSWIVDISNGFDPITLKQRRIVRKGFKTKKEATEAE
                     HYLRSVELKERFYGAKITVPMLYDMLKEEDSINHRKISYINTQENNYNKHIKEYFINV
                     DNVGKLTYEDIYQFREQLRQKIAQNTDKPLSTNTINKIMILLKKIFDVGLRKGYYTTN
                     PVKLVKKLPIEKTKMQFWTVKEFQQFLTLFEPEEYNIKLLFTVLFFTGLRLGEALALT
                     WQDIEFPSNTIYITKSVYVNRGVSHISTTKTKAGTRRIVINKKLSRELQDWQQHQKHL
                     LEQFTNDSLSLQVFQNSPIIITKNSIEKQYKKILERDNTLKKIRIHDFRHSHASLLIN
                     NGEDYLVVKERLGHASITTTIDTYSHLYPSKQKDLADKLDDLL"
     gene            3122..3577
                     /locus_tag="VYQ24_001680"
     CDS             3122..3577
                     /locus_tag="VYQ24_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1968796.1"
                     /translation="MDKIQKSVSQQAYEVAKKAHLGQVDKAGVDYIKHPKTVASFVET
                     DEEKAVAYLHDVVEDTKLTLLDLEELGFSKMIIEAVDILTKKKGQDYQSYLNLVKTNE
                     LARVVKLADLRHNSDLTRLSKITTKDIERNKKYRSAIAFLSQVEKNSNF"
     gene            complement(3647..3730)
                     /locus_tag="VYQ24_001681"
     tRNA            complement(3647..3730)
                     /locus_tag="VYQ24_001681"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3694..3696),aa:Leu,seq:caa)
     gene            complement(3740..3811)
                     /locus_tag="VYQ24_001682"
     tRNA            complement(3740..3811)
                     /locus_tag="VYQ24_001682"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3777..3779),aa:Gln,seq:ttg)
     gene            complement(3825..3897)
                     /locus_tag="VYQ24_001683"
     tRNA            complement(3825..3897)
                     /locus_tag="VYQ24_001683"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3862..3864),aa:His,seq:gtg)
     gene            complement(3912..3982)
                     /locus_tag="VYQ24_001684"
     tRNA            complement(3912..3982)
                     /locus_tag="VYQ24_001684"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3948..3950),aa:Trp,seq:cca)
     gene            complement(3987..4067)
                     /locus_tag="VYQ24_001685"
     tRNA            complement(3987..4067)
                     /locus_tag="VYQ24_001685"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4031..4033),aa:Tyr,seq:gta)
BASE COUNT         1530 a          737 c          714 g         1094 t
ORIGIN      
        1 attggtgact tacatttaaa atctcaacta attttaccca ttgttgacaa aattatccaa
       61 acacataaca ttaagagaat catttttctt ggtgattatg ttgatttaca tggtcaaact
      121 aacaatatcc aactttatgc caaagacctc acttttttat actcttggaa aatagataaa
      181 gagcaaaacg gaatagatgt aattaatctt ataggtaatc atgatgctta ttatttactg
      241 ggagaacaag ctcctttctc aattcaaaat ttagaagtgt ttttcgcagt aaaagaattg
      301 ttgcagaatt tgaaattaca aatagcttat caactagatg attatttagt cagtcacgct
      361 ggtttcaatc tgatatttga cccaaaagaa tggtatttta acctctatac caaagagcat
      421 gaagaagaat tagatattct agcttataca ataggaccaa tgcgtggtgg gaaagctctt
      481 ggaggttctc ctttatgggc acattttaga gaattagaac tgcttcccaa ccaagactat
      541 cctaaacaaa ttgttggaca tacccctaaa gaatctattg atatttctaa aaatgttatt
      601 ggtatagata ctttttcatt atatattgac aaagataata aatatcaatt tattggtaac
      661 ggagatttgc tagtttatga ccaaggacat tttgaggtca tttcaacaga ctgggcaaca
      721 gacaaaatat taaaacaatt accacgacta taaatttaat agctacttct catcatgtac
      781 ctaagcaaaa ctgccgacaa gcagtcggca agctggaagt gtgtttagcg gagcggtgct
      841 taggatcaca tgatagaagt agggaggagc tttgctcctc ctagtttatt tattatataa
      901 acaatctttt ggcgaaagct aatctaaaag gaactatttt ttatgacctc atataataaa
      961 aaaatagtcc gcacttcttc ctatattgaa atttgggaat atgaaaagcc aatattttca
     1021 aaaggaaaat ccattttaga tactaatact gataacaagg aaaaatcaaa acgaagaaca
     1081 tttgatgaac taacacccga agaacaagaa caacgtcttg aacgtatgaa gaagactcgc
     1141 ttagaagcta agtggaactt actccgtcta gttgattgta attttgatga tacaacttct
     1201 tttcttacat taaccactaa ggaaaatatc agcgatagaa accacttcaa agacttactc
     1261 aaaaccttta ttaaacgttt taactatcac atatttcaaa ctaaaaaaag caaattaaaa
     1321 tatatagcag tattagaaaa gcaaaaacgt ggagcatggc atgctcatat tctgcttttc
     1381 agcgttcctt atgtaccaca ccgtcaactc ttggaacttt ggggacatgg agcagtacgg
     1441 ataaataaag ttgatgtcga cagcaaagaa aaccgcggtc gatatgttac aaaatatttt
     1501 gaaaaaggta tcggacaaga gctattagaa aattttggaa aacaagccta tctgtcatct
     1561 cgcaacttga aaaaacctga tgaagataag ttttatacct acgaaacatt tgactatgac
     1621 agctcaactg tcctatatga aacggagtac actagtaaag tctataagga cggacagtat
     1681 ttcgataatc gcgttaccta caagaagata aagctagata aaaaggagta agtcacatga
     1741 tagcaactgt aaataagaat gacctagttg cccttggttt ctctgaggga acttcaaaac
     1801 gcattatccg acaaggaaaa gaacttctag tagcacgagg atttagggtg tatcaaaaca
     1861 aacggattgg aactatacct gctactatag ctactgaact actcgggttt gatgttcaaa
     1921 ataccccact tattaggggg taacagatgg caatacgaaa agacaaagac aatagctgga
     1981 tagttgacat cagtaacggt ttcgacccca tcacgttaaa gcaacgtaga attgttagaa
     2041 aaggatttaa gaccaagaaa gaagccaccg aagcagaaca ctacttacga agcgtagagc
     2101 taaaagaacg cttctacgga gcaaaaataa ctgttcccat gctttatgac atgttgaaag
     2161 aagaagacag cataaaccat agaaaaataa gctatatcaa tactcaagag aataactaca
     2221 acaagcatat caaagagtat tttataaacg tggataatgt tggaaaactg acctatgaag
     2281 atatctatca atttagagaa caactccgtc aaaaaatagc acaaaatact gataagcctt
     2341 taagtacaaa tacaattaac aaaatcatga tattgcttaa aaagatattt gatgtaggtt
     2401 tgcgtaaagg ctattacact acaaatcctg taaagctagt aaaaaaatta ccaattgaaa
     2461 aaactaaaat gcaattttgg acagtcaaag aatttcaaca gtttctaaca ctgtttgaac
     2521 cagaagaata caatatcaaa ttactcttta cagtcctatt ctttacaggt ttaaggcttg
     2581 gagaagcact tgcactaaca tggcaagata tcgaatttcc aagcaataca atttatatca
     2641 caaaatctgt ctatgtcaat agaggggtta gccatatcag cacaactaag actaaagcag
     2701 ggacaagaag aattgttatc aataaaaaat taagtcgaga attacaagat tggcaacaac
     2761 atcaaaaaca tctgttagag caatttacca atgatagcct aagtctacaa gtgtttcaaa
     2821 atagcccaat cataattact aaaaactcca ttgagaaaca gtacaagaaa atacttgaac
     2881 gtgataacac actaaaaaag atacgcatac acgattttag acactcacat gcttctcttt
     2941 taatcaataa tggagaagat tacctagtgg ttaaagaacg cctaggacac gcttctataa
     3001 cgaccacaat cgatacctat tctcacttgt atccaagcaa gcagaaagac ctagcagaca
     3061 aactagatga tttattgtaa agtagcttgc atttttaaaa actaaaatat aaaataaaga
     3121 tatggataaa atacaaaaat cagtaagcca acaggcttat gaagtagcta aaaaagctca
     3181 tttaggacag gttgataaag caggtgttga ctatattaaa caccctaaga ccgttgcaag
     3241 ttttgtagaa actgatgagg aaaaagcggt tgcttatctt catgatgttg tagaagatac
     3301 taaactaacc ctactagacc tagaagagct aggcttttca aagatgatta tagaagccgt
     3361 tgatatactt acaaagaaaa aaggacagga ttatcaatca tatctcaacc tagtaaagac
     3421 aaatgaactc gctagggttg taaaattggc tgacctacgt cataattcag atttaacacg
     3481 actttcaaag ataactacaa aggatataga gagaaataaa aaatatcgtt ctgctatcgc
     3541 ttttctaagc caagtagaaa aaaatagtaa tttttagtaa ctcaatcaag aacgattcaa
     3601 aatataaaag aaaagacaag gcttagaaac cttgtcttta ttactatacc ggcggccggg
     3661 gtcgaaccgg cacgtccttg cggacactgg attttgagtc cagcgcgtct gccaattccg
     3721 ccacgccggc aaatagtaac tggggtagct ggattcgaac caacgcatga gggagtcaaa
     3781 gtcccttgcc ttaccgcttg gctatacccc aataatataa aataggcgag tgatggggat
     3841 cgaacccacg catgccagag ccacaatctg gtgtgttaac cacttcacca cacccgccat
     3901 aattctatta acacgggcag taggaattga acccacactg aaggttttgg agaccttagt
     3961 tctaccttta aactatgccc gtaaaatgga aggggaggga ttcgaacccc cgaacccgaa
     4021 ggagcggatt tacagtccgc cgcgtttagc ctcttcgcta cccttccaaa atata
//
LOCUS       DAVMEI010000136         4044 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVMEI010000136 DAVMEI010000000
VERSION     DAVMEI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.136"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(26..223)
                     /gene="blpI"
                     /locus_tag="VYQ24_001686"
     CDS             complement(26..223)
                     /gene="blpI"
                     /locus_tag="VYQ24_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV1968797.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            512..1099
                     /locus_tag="VYQ24_001687"
     CDS             512..1099
                     /locus_tag="VYQ24_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1968798.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1026..2669
                     /locus_tag="VYQ24_001688"
     CDS             1026..2669
                     /locus_tag="VYQ24_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1968799.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLVQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2680..4041
                     /locus_tag="VYQ24_001689"
     CDS             2680..4041
                     /locus_tag="VYQ24_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1968800.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
BASE COUNT         1331 a          817 c          638 g         1258 t
ORIGIN      
        1 aatactatat ttaagaatca taatcttaca aagaggacat aattcctgaa cctacacaaa
       61 taagtgttgc tgctccccca gttatcggac cagtcgcagc agctaatagt actgctccaa
      121 tacaaccacc gattgcagat cctaaattgc ctcttcctcc actaactatt tcgagttctt
      181 cattatccat aacagaaaat tgttccatca tttttgtatt catgacaaat actccttttt
      241 tcttttttct ttttttattt ttgtcttgtt gtaactttga taagtttagt atatcatcgt
      301 tttttaaaat ttttcatcca gatcttgaat tgtcatcgaa acgtcttgaa ttaggttttt
      361 tatttcaagc cacctctaaa tgtttaaaaa aaataatttc taatcacttt tttaccattc
      421 aggaagtttt aatgactatt caagatttca taaaatatga acttagtttt atgacataat
      481 agacctatcc actatatgaa aggaattgcc aatgacttct tataaacgta catttgttcc
      541 tcaaatagat gcgagagact gtggtgtcgc tgccttagcc tcgattgcta aattctatgg
      601 ttcagatttt tctctagctc acttgagaga acttgcaaag accaataaag aagggacgac
      661 tgctcttggc attgtaaaag ccgctgatga aatgggcttt gaaacaagac ctgttcaagc
      721 agataaaacg ctctttgaca tgagtgatgt cccctatcca tttatcgttc acgttaacaa
      781 agaaggaaaa ctccaacatt actatgttgt ctatcaaaca aagaaagact atctgattat
      841 tggtgatcct gacccttctg taaaaatcac taaaatgtca aaagaacgct ttttctatga
      901 atggactgga gtagctattt ttctagctac caaacccagc tatcaacccc ataaagataa
      961 aaagaatggt ctactaagca agcttccttc ctctgatttt caaacaaaaa tctctcattg
     1021 cttacattgt tctctcaagc ttattggtca ctattatcaa tataggtggt tcttactatc
     1081 tccaaggaat cttggatgaa tacattccaa atcagatgaa atcaacttta ggaatcatct
     1141 cagttggtct ggttatcacc tatatcctcc aacaagtcat gagcttctcc agagattatc
     1201 tcctaaccgt tctgagtcag agattaagta ttgatgtgat tttatcctat attcgccata
     1261 tttttgaact tcccatgtct ttctttgcga cacgtcgtac aggagaaatc atttcacgat
     1321 tcacagatgc taactctatt atagatgcct tggcttctac cattctttct ctttttctgg
     1381 atgtttctat tctgattctt gtaggaggcg tcttactggt acaaaaccct aatctcttcc
     1441 ttctttctct tatttccatt cctatataca tgttcatcat cttttctttt atgaaacctt
     1501 tcgaaaaaat gaaccatgat gtcatgcaaa gtaattctat ggttagctct gccattatcg
     1561 aagatatcaa cgggattgaa actataaagt cgctcacgag tgaagaaaat cgctatcaaa
     1621 atatagacag cgaatttgta gattatttgg aaaaatcctt taagctcagt aaatattcta
     1681 ttttacaaac gagtttaaag cagggaacaa aattagttct gaatatcctt atcctatggt
     1741 ttggcgctca attagtcatg tcgagtaaaa tttctatcgg tcagctgatt acctttaaca
     1801 cacttttttc ttactttaca actcctatgg aaaatattat caacctccaa accaaactcc
     1861 aatctgcgaa ggtcgctaat aaccgtttga acgaagtcta tctagtcgaa tctgaatttc
     1921 aagttcaaga aaaccctgtc cattcacatt ttttgatggg cgatattgaa tttgatgacc
     1981 tttcttataa gtatggtttt ggacgagata ccttaacaga tattaatctc acgattaaac
     2041 aaggagataa ggttagccta gttggagtta gtggttctgg taaaacaact ttagccaaaa
     2101 tgattgtcaa tttctttgaa ccctacaaag gacatatttc tatcaatcat caggatatta
     2161 aaaacattga taaaaaagtc ttgcgccgtc atattaatta cctaccacaa caagcctata
     2221 tctttaatgg ctctatcttg gaaaacttaa ctttgggcgg taatcatatg attagccaag
     2281 aagatattct aagagcttgt gaattagctg aaattcgtca agacattgaa agaatgccta
     2341 tgggctatca aactcagctc tctgatggag ctggtctatc aggaggacaa aagcaacgaa
     2401 tagccctcgc tcgtgctctt ttaactaaag ctcctgtttt aatactagac gaagctacta
     2461 gcggtcttga tgtcttgact gagaaaaagg ttatagataa tcttatgtct ctaactgata
     2521 aaaccattct ctttgtagcc catcgtctca gtatagccga acgaaccaac cgtgtcattg
     2581 ttcttgacca ggggaaaatc attgaagttg gtagtcacca agagttaatg caggcgcaag
     2641 gtttctacca tcatctattc aataaataag gagaatgtca tgaatcctaa tctttttaga
     2701 agcgtcgagt tttatcagag acgttaccat aactatgcga cagtgttcat tatacctctt
     2761 tcattactat ttactttcat cttgattttc tcccttgttg ccacaaaaga aattactgtt
     2821 acttcccaag gagaaatcgc ccctacaagt gtcattgcct ccattcagtc aaccagtgat
     2881 aatcctatcc tagctaatca tttagtggca aatcaagtag ttgaaaaagg ggacttactc
     2941 atcaaatact ctgaaacaat ggaagaaagt cagaaaactg ccttagcaac tcaattacaa
     3001 agacttgaga agcaaaaaga aggacttgga attttgaaac aaagcttaga aaaagcgact
     3061 gatctttttt ctggcgagga tgaatttggc taccataata cctttatgaa ttttactaaa
     3121 caatcccatg atattgaact gggtatcaca aagactaaca ccgaagtttc aaatcaagct
     3181 aatctttcca atagcagttc atcagctatt gaacaagaaa ttacaaaagt tcaacaacaa
     3241 attggagaat atcaagagtt gagagatgct atcataaata acagagcacg cttaccaact
     3301 ggcaatccgc accagtcaat tttgaatcgt tatcttgtag cctcacaagg acaaacacaa
     3361 ggaactgcag aggagccatt tttatctcaa attaatcaaa gtattgcagg tcttgaatca
     3421 tctatcgcaa gcctcaaaat tcagcaagct ggtatcggaa gtgtagcaac ttatgataac
     3481 agtttagcaa ccaaaattga agtactccgc actcagtttt tacagacagc ctcacagcaa
     3541 caactaactg tggagaatca attaacagaa ttaaaagtac aactagatca agccacacag
     3601 cgtttggaaa acaatacctt aacctcccca agtaaaggta tcgttcatct gaacagcgaa
     3661 tttgaaggta aaaatagaat tccaactggt acagaaattg ctcaaatatt ccctgtcatc
     3721 acagatacaa gagaagtact aatcacttac tacgtatctt ctgactatct acctctacta
     3781 gataaaggac aaactgtaag attaaaactg gagaagattg gaaatcacgg caccaccatc
     3841 atcggccaac ttcagacaat tgatcaaact cctaccagaa cagagcaagg aaatctcttt
     3901 aaattaaccg ctcttgcaaa actatctaac gaggatagta aactcatcca atatggctta
     3961 caaggtcgcg tcactagtgt aactacaaag aaaacatatt ttgattattt caaagataaa
     4021 attttaaccc attctgatta attt
//
LOCUS       DAVMEI010000137         4007 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVMEI010000137 DAVMEI010000000
VERSION     DAVMEI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.137"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(162..1832)
                     /locus_tag="VYQ24_001690"
     CDS             complement(162..1832)
                     /locus_tag="VYQ24_001690"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1968801.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2062..2751
                     /gene="coaB"
                     /locus_tag="VYQ24_001691"
     CDS             2062..2751
                     /gene="coaB"
                     /locus_tag="VYQ24_001691"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1968802.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2763..3314
                     /gene="coaC"
                     /locus_tag="VYQ24_001692"
     CDS             2763..3314
                     /gene="coaC"
                     /locus_tag="VYQ24_001692"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1968803.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3298..3861
                     /locus_tag="VYQ24_001693"
     CDS             3298..3861
                     /locus_tag="VYQ24_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1968804.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         1215 a          891 c          719 g         1182 t
ORIGIN      
        1 gaactgacag agtcagtatc atatacctac ggcaaggtga agctgacgta gtttgaaaag
       61 attttcgaag agtataaatc ttattgatga actgcttgca gtctgagaaa aaatgagctt
      121 ggatattatt tccaaactca cttaaagtca atttcaatcc actagaacaa gcctagtaca
      181 gttccatcgc tttcaacatc catgttgaga gctgctggac gttttggaag acctggcatg
      241 gtcataacat caccagttaa ggcaacgatg aagcctgcac ctaattttgg taccaattca
      301 cgaatggtaa tttcaaagtt ttctggtgct ccaagcgcat ttggattgtc tgagaaactg
      361 tattgagttt tagccataca aattggcaat ttgtcccaac cgttttgaac gatttgagca
      421 atttgtgttt gagctttctt ctcaaagttc actttgctac cacgatagat ttcagtgaca
      481 attttttcaa tcttttcttg gacagaaagg tcattatcgt acaaacgttt atagttagct
      541 ggattttcag caattgtctt aacaactgtt tcggcaagtg ctactccacc ttctgctcca
      601 tcagcccaga cactagccaa ttcaactggt acatcgattg aggcacagag ttcttttaag
      661 actgcaattt cagcttctgt atcagataca aattcgttaa tagctacaac tgctggaata
      721 ccgaacttac ggatattttc aacgtggcgt ttcaagttag caaaacctgc acgaactgcc
      781 tctacatttt cttcagtcag agcgtcttta gccacaccac cattcatctt aagggcacga
      841 agggttgcga caataacaac tgcatctgga gatgttggca agtttggtgt cttgatatca
      901 aggaatttct cagcaccaag atccgcacca aaaccagctt cagtaacagt gtaatcagcc
      961 aagtgaaggg ctgttgtcgt cgccaaaaca gagttacagc catgagcgat attggcaaat
     1021 ggaccaccat gtacaaaggc aggtgtaccg taaattgtct gaaccaagtt tggcttaata
     1081 gcatccttca aaatcaaagc caaggcaccc tcaacctgca aatcacctac agaaacaggc
     1141 gtacggtcat agcgataacc aataacgata ttcgccaaac gacgtttcaa gtcctcgatg
     1201 tccgttgcca agcaaagaat tgccatgatt tctgaagcaa ctgtaatatc aaaaccatcc
     1261 tcacgtggaa taccgtttag aggaccacca agaccaacag tcacatggcg gagcgcacgg
     1321 tcgttcaagt ccacaacgcg tttccagagg atacgacgtt gatcaatttc cagctcattc
     1381 ccttggtgca agtggttgtc aatcaaggca gaaagggcat tgttggcagt tgtaatagca
     1441 tgcatatctc cagtaaagtg gaggttgatg tcttccattg gcagaacttg tgcataccca
     1501 ccaccagcag caccaccctt gatccccatg actggaccaa gagacggttc gcggatagca
     1561 atcatggttt tcttgccaat cttgttcaag gcatccgcaa gaccaatggt aagcgtcgac
     1621 tttccttcac ctgcaggtgt tgggttgatg gcagtaacca agatcaattt accgactgga
     1681 ttgctctcaa ctgcacgaat tttatcaaag ctgagtttag ccttgtactt tccgtacaac
     1741 tccaaatcgt cgtaagaaat accaagtttc tctacaacat caacaattgg cttcaactca
     1801 atactctgtg cgatttcaat atctgttttc attcaaaatt cctctaacct cttatatgat
     1861 aattcattat atcacaaaac aagattttta acatcctaaa actctctaaa cgttcgtaaa
     1921 tatctctgtt tttaagactt ttagagtcct ttcttaaatt ttatatggct ttatagtttg
     1981 aaactataat aaatcttcgt ttttaccaaa aatttatcac tttcatttta cttaccgctt
     2041 atttttgtgt acaatagtgc tatgaaaatt ttagttacat cgggcggtac cagtgaagct
     2101 atcgatagcg tccgctctat cactaaccat tctacaggtc acttggggaa aattatcaca
     2161 gagactttgc tttttgcagg gcatgaagtt tgtttaatta cgacaaaacg agctctgaag
     2221 ccagagcctc atcctaacct aagtattcga gaaattacca ataccaagga ccttctaata
     2281 gaaatgcaag aacgtgttca ggattatcag gtcttgatcc actcaatggc tgtttctgac
     2341 tacactcctg tttatatgac agggcttgag gaagttcagg ctagctccaa tctaaaagaa
     2401 tttttaagca agcagaatca tcaggccaag atttcttcaa ctgatgaggt tcaggttttg
     2461 ttccttaaaa agacacccaa aatcatatcc ctagtcaagg aatggaatcc tactattcat
     2521 ctgattggtt tcaaactgct ggttgatgtt accgaagatc atctggttga cattgcacga
     2581 aaaagtctta tcaagaatca agcagattta atcatcgcga atgacctgac tcaaatttca
     2641 gcaggtcagc accgagctat atttgttgag aaaaatcagc ttcaaacagt ccagactaaa
     2701 gaagaaattg cagaactcct ccttgaaaaa attcaagcct atcattctta gaaaggaaaa
     2761 ctatggcaaa cattctcttg gctgtaacgg gttcaatcgc ctcttataag tcggcagatt
     2821 tagtcagttc tctaaaaaaa caaggccatc aagtcactgt cttaatgact caggctgcta
     2881 cagagtttat ccaacctttg acactacagg tactctcaca gaatcctgtc cacttggatg
     2941 tcatgaagga accctatcct gatcaggtca atcatatcga acttggaaaa aaagcagatt
     3001 tatttatcgt ggtacctgca actgctaaca ctattgcaaa actagctcac ggatttgcgg
     3061 acaacatggt aaccagtaca gctctagccc taccaagtca tattcccaaa ctaatagctc
     3121 ctgctatgaa tacaaaaatg tatgaccatc cagtaactca gaataatctg aaaacattag
     3181 aaacttacgg ctatcagctg attgctccta aggaatccct actagcttgt ggagaccacg
     3241 gacgaggagc tttagctgac ctcacaatta ttttagaaag aataaaggaa actatcgatg
     3301 aaaaaacgct ctaatattgc acccattgct atcttttttg ctaccatgct cgtgatacac
     3361 tttctgagct cacttatctt taaccttttt ccatttccaa tcaaaccgac cattgttcat
     3421 attcctgtca ttattgccag cattatttat ggtccacgag ttggggttac acttggattt
     3481 ttgatgggat tacttagctt gacggttaac acgattacga ttctaccgac aagctacctc
     3541 ttctctccct tcgtaccaaa cggaaacatc tactcagcta tcattgccat tgtcccacgt
     3601 attttgattg gtttaactcc ttacttagtc tataaactga tgaaaaacaa gactggtctg
     3661 attttagctg gggcccttgg ttccttgaca aatactatct ttgtccttgg aggaatcttc
     3721 ttcctatttg gaaatgttta taatggaaat atccaacttc ttctggcaac cgttatctca
     3781 acaaattcaa ttgctgaatt ggtcatttct gcaattctaa ccctagccat tgttccacga
     3841 ctacaaacct tgaaaaaata aaaacaatct ccgctttcaa acctgaaggt ggagattttg
     3901 ctctatacag tttcttttta atgaaatctt tactattagt aaaacgaaat aagaacagga
     3961 caaatcgatc aggacagcca aatcgatttc taacaatgtt ttagaag
//
LOCUS       DAVMEI010000138         3931 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVMEI010000138 DAVMEI010000000
VERSION     DAVMEI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.138"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(68..139)
                     /locus_tag="VYQ24_001694"
     tRNA            complement(68..139)
                     /locus_tag="VYQ24_001694"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(104..106),aa:Arg,seq:cct)
     gene            complement(182..529)
                     /gene="rplS"
                     /locus_tag="VYQ24_001695"
     CDS             complement(182..529)
                     /gene="rplS"
                     /locus_tag="VYQ24_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1968805.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(648..977)
                     /gene="crcB"
                     /locus_tag="VYQ24_001696"
     CDS             complement(648..977)
                     /gene="crcB"
                     /locus_tag="VYQ24_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1968806.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(971..1345)
                     /gene="crcB"
                     /locus_tag="VYQ24_001697"
     CDS             complement(971..1345)
                     /gene="crcB"
                     /locus_tag="VYQ24_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1968807.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1342..1608)
                     /locus_tag="VYQ24_001698"
     CDS             complement(1342..1608)
                     /locus_tag="VYQ24_001698"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1968808.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKCSRDYQDQNIK"
     gene            complement(1724..2167)
                     /locus_tag="VYQ24_001699"
     CDS             complement(1724..2167)
                     /locus_tag="VYQ24_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1968809.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            2271..3206
                     /locus_tag="VYQ24_001700"
     CDS             2271..3206
                     /locus_tag="VYQ24_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1968810.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3260..3544
                     /locus_tag="VYQ24_001701"
     CDS             3260..3544
                     /locus_tag="VYQ24_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1968811.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3601..3819)
                     /locus_tag="VYQ24_001702"
     CDS             complement(3601..3819)
                     /locus_tag="VYQ24_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968812.1"
                     /translation="MSISPRFETLEQAIAFKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3816..>3931)
                     /locus_tag="VYQ24_001703"
     CDS             complement(3816..>3931)
                     /locus_tag="VYQ24_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1968813.1"
                     /translation="TLEDVMNQLQDVIQGLEKEVIKSIVNRRWNRMLCESR"
BASE COUNT         1220 a          882 c          711 g         1118 t
ORIGIN      
        1 aacttgagaa aattgccttc aaataaacat tttgaatttt ctttgtcttt ctttacatta
       61 ctaaaaatgc cccctgcagg aatcgaacct gcaactactc cttaggaggg agttgttata
      121 tccattgaac taagggagct agataaaaac tctgctgaaa aagcagagtt ttttagtcga
      181 attaacgacg gatttctttg atacgagctg ccttaccttg aagagcacgc aagtagtaca
      241 atttcgcacg acgtacttta ccgtaacgaa caacttcgat tttttcaaca cgtggagtgt
      301 ggattgggaa gatacgctca acacctacac cgttagagat tttacgaact gtgtagtttt
      361 ctgagattcc agcaccttta cgtgcgataa caacaccttc aaaaatctgg atacgttcac
      421 ggttaccttc gacaactttc gcatgtacac gaacagtgtc accaggacgg aatgatggga
      481 tatctgtacg aagttgacct tcagtcaagc tttggattaa tggattcatt ttattctcct
      541 atctttgtca atcttgagga aacttcctca gcggataaac tgtatttttg tgcgtccatc
      601 acacacaaga tacagtctac caaattttca caaaaaagta aagaaattta tagcaaaatt
      661 cctaaaaaaa tcgcaaccaa accacctatg taagtcaaag tcaggtaaga ataaaagacc
      721 ttcttatcac ttagcagtct ttgaagttcg tcattcaagg tcgaaaaagt tgttaaaccg
      781 ccacaaaatc ctgttgctag aatagcatat acttccttag actctacatg attgtagaat
      841 actccaatta aaaaacaacc taaaagatta gctatgagcg ttccgagtgg caatttagaa
      901 gcttgattgt agcgggaaaa gaaataccgt acgagtgctc ccaagccaca ggcgattgca
      961 agatagacga ttaccatttc ttcctcccta gaaaataagc taagagtagg cctccaccga
     1021 tgctcaaaag caaatacagt atcaaactaa gataacgccc tgtatcaagc agcttcacag
     1081 tatcaagcat taggctggaa aaagttgtta aacctccgca aaaacccgtc cccagtgcta
     1141 aaatcaagcc cttactagtc cccttataga ccagatagcc cttgacaaga taaatcaagc
     1201 agaaaattcc cagatagttg acaaagagag tgccccaagg gaaatctgga ctggctggta
     1261 accaagtgga aactaggtag cggacaagtc cgcccaacat agcagctaga aaaatcccta
     1321 gcggataaaa ttgttctttt ttcatttgat attttgatcc tgataatcac gcgaacattt
     1381 gagtatgtcc gaaaaagtcg cgacaatagt ctcctgatag cgcttgtcct ctacacgact
     1441 tctaaccttt tcaaaaataa ctgcttctct cttagtatct aaaatcggtt tccctgaagc
     1501 cttcttataa gcgacaactc cttcaactaa gtgcattcgt tcttctaaca gtttgacaat
     1561 ttggtcgtcg atttgatcga tttcttgacg aataatatct aaatccatac agtctcctcc
     1621 tttatttgaa ttattgtatc aaaaagccat caattaggct agtaaaatcc caactctcga
     1681 taagaaaaat gcactgattg attacatcag cgcactttta tgcttatcct actttagcaa
     1741 ccaattcttc tgcgaattgt tccaagcgtt caatatcttc ttcctcggca gaaagatcaa
     1801 ctttaacaca ctctgaacct ttttctgctc ctgttgacac aaagacacgg tcaaagtcat
     1861 caacagcctt acagaattca tcgtagaagg tatctcctga gccgaccact ccgtagattt
     1921 tgccattcaa gttgagatct gctaggtctt cgtagaagtc catcatctca tctggcaatt
     1981 caccatcacc ataagtatag gtcgcaacga tagcgatgtc tgcttccaag aagtctgaag
     2041 cgtcaacagt tgtacattca tcaacatcaa catccaagcc caagtcacgt aatttgtctg
     2101 ctacaatatc tgcaatttct tcggtattac cggtcatact ggcaaataca atttttgcta
     2161 atgccatatc gtcctcctca atttatcttt ctccattata tcacatcttt ttcattttaa
     2221 aaataaaaat tcttgtgcta caatgaaatg agaaagaatt gaggttattt atggagattt
     2281 gccaacaaat tttagaaaaa attaaagaat acgacaccat tatcattcat cgtcatatga
     2341 aaccagaccc tgatgccttg ggaagtcagg tgggattgaa agccttgcta gaacatcatt
     2401 tcccagaaaa aaccatcaaa gccgtcggtt ttgatgaacc aactcttact tggatggctg
     2461 agatggatct tgttgaagat agagcctacc aaggcgcact tgtcatcgtc tgtgatacag
     2521 ctaatactgc tcgtatcgat gataagcgct atagtcaagg tgattttctc attaagattg
     2581 accaccatcc aaatgatgat gtatacggtg acctgtcttg ggtcgatact agttcaagta
     2641 gcgctagcga gatgattacc ctatttgccc aaacaaccca actagccttg gcagatcgcg
     2701 atgctgagtt gctctttgca ggaattgtcg gtgatacagg tcgcttcctc tacccttcta
     2761 ccactgcacg gactcttcgc ctggctgctt atttgagaga acataacttt gactttgcag
     2821 ctctcactcg caaaatggac actatgagct acaaaattgc taaactgcaa ggctacatct
     2881 acgaccatct ggaagtggat gaaaatggtg ctgctcgcgt tatcctgagt caagaagtct
     2941 tgaaacaata caatgtaacc gatgctgaaa ctgcagccat tgtcggtgca cctggacgca
     3001 ttgacagagt gagtctctgg ggaatttttg tcgaacaggc tgatggccac taccgagttc
     3061 gcttacgcag taaaatccat cctatcaatg aaatcgccaa ggagcatgat ggtggaggcc
     3121 accctctagc aagtggtgct aattcctata gcctagaaga aaatgaaatc atctaccaaa
     3181 agttaaaaaa cttgcttaaa aactgataaa atacttgcca aacttttcag aatctgatag
     3241 actagtatgg taacaatcta tggctcgcaa agagaccatg gcagaaagga aatattgcaa
     3301 aatgaaaaaa gatatccatc cagaatatcg cccagttgtc ttcatggaca caactactgg
     3361 ttaccaattc cttagcggtt caacaaaacg ctctaacgaa acagttgagt tcgaaggcga
     3421 aacttaccca ttgatccgtg tggaaatttc atcagactca cacccattct acactggacg
     3481 tcaaaagttc actcaagcag atggacgcgt ggatcgtttc aacaaaaaat acggtctcaa
     3541 ataatgataa aagaacagtt tcgactgttc ttttttgttt cttgaaatca actgctgttt
     3601 tcatgttcca gactcatctg taggttcggt ttccatgcta ctaggcagga aggaaatagc
     3661 tgtttcaaca cgtccataat gagctgtact attgtcacga accacacttt cattgatggt
     3721 ccaagtggaa ttcattttct taaaagcttc tcggactttt tccaaatctt tgaaggcaat
     3781 ggcctgctct aaggtttcaa aacgaggact tatactcatc tgctttcaca aagcattcta
     3841 ttccatctcc gattaacgat ggactttatc acctccttct ccagtccttg tatgacatct
     3901 tggagttgat tcatgacatc ttccaaagtt c
//
LOCUS       DAVMEI010000139         3904 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVMEI010000139 DAVMEI010000000
VERSION     DAVMEI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.139"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            78..245
                     /locus_tag="VYQ24_001704"
     CDS             78..245
                     /locus_tag="VYQ24_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEV1968814.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(305..658)
                     /locus_tag="VYQ24_001705"
     CDS             complement(305..658)
                     /locus_tag="VYQ24_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1968815.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1163..1585
                     /locus_tag="VYQ24_001706"
     CDS             1163..1585
                     /locus_tag="VYQ24_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1968816.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            1582..1812
                     /locus_tag="VYQ24_001707"
     CDS             1582..1812
                     /locus_tag="VYQ24_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1968817.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            2273..2476
                     /locus_tag="VYQ24_001708"
     CDS             2273..2476
                     /locus_tag="VYQ24_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEV1968818.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            2558..3775
                     /locus_tag="VYQ24_001709"
     CDS             2558..3775
                     /locus_tag="VYQ24_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1968819.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
BASE COUNT         1360 a          658 c          770 g         1116 t
ORIGIN      
        1 aagatgaaat attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta
       61 taaactacaa gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg
      121 aagatactga attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa
      181 ttgaaataaa gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc
      241 gataaaatga gattaataca atctcatttt atcggctctt tccgttatgt atggattctt
      301 ttaattagtc ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattaagg
      361 atagttcttt gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt
      421 ttaccaagtt attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa
      481 ggtcataaag aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc
      541 gtgggtcaat ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag
      601 cttctttaat ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag
      661 tagaccacct ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt
      721 gttatatggt ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa
      781 ccgttatatg gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg
      841 tttgttggag gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg
      901 gttcttgtca aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt
      961 ataaatcata tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg
     1021 cttttttggc tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc
     1081 cctttcacat tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct
     1141 agacagaaag ggggtgagaa acatgaaacc atcttctttt cagaccacaa tagaaaatca
     1201 gtttgactat atctgtaaac gtgctatgga agacgagcga aagaattata tgctttatct
     1261 ttcaaggatt gcaaagcgtg aggtgtcctt ttcggatgtt ggcgattatc ttgttagcca
     1321 gtttgcgaca acagataact attcaactga ctttcagatt tttacactca atgggttatc
     1381 agtaggcgtt gaaaatgatt tgttgagtga agcattacgt gagttgccag acaagaaacg
     1441 tgaaattcta ctgctgtttt actttatgga catgagcgat tcagaaattg cagacctgtt
     1501 gaaattgaac cgttctactg tctatcggca tagaaccagt ggactagcct taattaaaaa
     1561 gtttatggag gaatttgaag aatgaaaaca caatatccta tgattccctt tcctctcatt
     1621 gtaaaggcaa cagatggcga taccgaagcg attaaccaga ttctacatca ttacagaggg
     1681 tacataacga agcgttccct acgacttatg aaagatgaat atggcaatca aagtatggtc
     1741 gttgatgaag tcttacgtgg aagaatggaa accagactga ttacaaagat tttgtcattt
     1801 gaaattaagt aatatcctct ctcctttcgt ggaagcgtgc taaaccattc cacgcttccc
     1861 gaacagggag gtttgttatt ccaccaaagc atattgagct ttcaatgtgt tttgataggc
     1921 taacgagcca ttgttctttg aaaactgaat aaaagtaatc gaatacgttt cgataagaaa
     1981 agagccaacg gaactaaccg ccatgaccta tcttataaag atagcgagcg attcatgtta
     2041 gtgatccgag aagcaatctt tagcaggatt gcctgcaacg acattcttat cgtgataatg
     2101 atactcccat acagtcaata gtccgagcgt gataaaaccg tcgcaggcaa tgagtatggc
     2161 tacatgagaa ccatgcaggg gtggaactcc cgtgagcttt gctaaagctg ttcgattgct
     2221 ggtaaaacaa cttttatgaa atccaaataa gtgatttgga aaggaggatt ttatgaagca
     2281 gactgacatt cctatttggg aacgttatac cctaaccatt gaagaagcgt caaaatattt
     2341 tcgtattggc gaaaacaagc tacgacgctt ggcagaggaa aataaaaatg caaattggct
     2401 gattatgaat ggcaatcgta ttcagattaa acgaaaacaa tttgaaaaaa ttatagatac
     2461 attggacgca atctagcgtc gccaaagggt cttgtatatg ataaaatagt attaagtcgt
     2521 atcaaggctc tttccataaa ggaaaggagc aaatgccatg tcagaaaaaa gacgtgacaa
     2581 taaaggtcga atcttaaaga ctggagagag ccaacgaaaa gacggaagat acttatacaa
     2641 atatatagat tcatttggag aaccgcaatt tgtttactcg tggaaacttg tggctacaga
     2701 ccgagtacca gcaggaaagc gtgattgtat ctcacttaga gagaaaatcg cagagttaca
     2761 gaaagacatt catgatggta ttgatgttgt aggaaagaaa atgacactct gccagcttta
     2821 cgcaaaacag aacgctcaaa gaccaaaggt tagaaaaaac actgaaactg gacgcaaata
     2881 tcttatggat attttgaaga aagacaagtt aggtgtaaga agtattgaca gtattaagcc
     2941 atcagacgct aaagaatggg ctattagaat gagtgaaaat ggttatgctt atcaaaccat
     3001 caataactac aaacgttctt taaaggcttc attctatatt gctatacaag atgattgtgt
     3061 tcggaagaat ccatttgact ttcaactgaa agcagttctt gatgatgata ctgtccctaa
     3121 gaccgtacta acagaagaac aggaagaaaa actgttagcc tttgcaaaag ctgataaaac
     3181 ctacagcaaa aattatgatg aaattctgat actcttaaaa acaggtcttc gtatttcaga
     3241 gtttggtggt ttgacacttc cagatttaga ttttgagaat cgtcttgtca atatagacca
     3301 tcagctattg agagatactg aaattgggta ctacattgaa acaccaaaga ccaaaagtgg
     3361 cgaacgtcaa gttcctatgg ttgaagaagc ctatcaagca tttaagcgag tgttagcgaa
     3421 tcgaaagaat gataagcgtg ttgagattga tggatatagt gatttcctct ttcttaatag
     3481 aaagaactat ccaaaagtgg caagtgatta caacggcatg atgaaaggtc ttgttaagaa
     3541 atacaataag tataacgagg ataaattgcc acacatcact ccacatagtt tgcgacatac
     3601 attctgtacc aactatgcaa atgcaggaat gaatccaaag gcattacagt acattatggg
     3661 acatgctaat atagccatga cgctgaacta ttacgcacat gcaacattcg attctgcaat
     3721 ggcagaaatg aaacgcttga ataaagagaa gcaacaggag cgtcttgttg cttagtagta
     3781 caaatgaatt tactacttat ttaccacttc tgacagctaa gacatgagga aatatgcaaa
     3841 gaaacgtgaa gtatcttcct acagtaaaaa tactcgaaag cacatagaat aaggctttac
     3901 gagc
//
LOCUS       DAVMEI010000140         3900 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVMEI010000140 DAVMEI010000000
VERSION     DAVMEI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.140"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            37..1326
                     /locus_tag="VYQ24_001710"
     CDS             37..1326
                     /locus_tag="VYQ24_001710"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1968820.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRAYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            1317..1397
                     /locus_tag="VYQ24_001711"
                     /pseudo
     CDS             1317..1397
                     /locus_tag="VYQ24_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            1469..1816
                     /locus_tag="VYQ24_001712"
     CDS             1469..1816
                     /locus_tag="VYQ24_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968821.1"
                     /translation="MIDKVVRNLLLTFFLCKMTKIINFLTTILVKKKKMCYNVSKLRE
                     KKKGNAEMSRLLQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQ
                     RIEDIQTVEFDGE"
     gene            1826..2032
                     /locus_tag="VYQ24_001713"
     CDS             1826..2032
                     /locus_tag="VYQ24_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968822.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            2032..2229
                     /locus_tag="VYQ24_001714"
     CDS             2032..2229
                     /locus_tag="VYQ24_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968823.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            2232..2966
                     /locus_tag="VYQ24_001715"
     CDS             2232..2966
                     /locus_tag="VYQ24_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1968824.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            2978..3619
                     /locus_tag="VYQ24_001716"
     CDS             2978..3619
                     /locus_tag="VYQ24_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1968825.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            3637..3867
                     /locus_tag="VYQ24_001717"
     CDS             3637..3867
                     /locus_tag="VYQ24_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968826.1"
                     /translation="MKRVILVAVIQAVILFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT         1123 a          730 c          853 g         1194 t
ORIGIN      
        1 gtaaaataag actattaagt ttaaagagga ttccctatga aattacaaaa accaaaagga
       61 acgcaggata ttttacctgc tgaatctgcc aagtggcagt acgttgaggg ctttgcccgt
      121 gagattttca agcgctataa ctatgcagaa gtgcgcacgc ctatttttga gcattacgag
      181 gttatcagtc gctctgtcgg agatacaacg gatatcgtaa ccaaggaaat gtacgatttt
      241 tatgacaagg gtgaccgcca tattaccctc cgtccagaag gaactgcgcc cgttgtccgt
      301 gcctatgtgg aaaataaact cttcgcccca gaagtgcaaa agccaagcaa gttctactat
      361 atgggaccta tgttccgtta tgagcgtcca caggcagggc gcttgcgcca attccaccag
      421 attggtgttg agtgttttgg ctctagcaat ccagctaccg atgtggaaac aatcgttatg
      481 gcagcccatt ttttgaagga aatcggtatt caaggtgtca aattgcacct caacactctt
      541 ggaaatcctg agagccgtgc agcctaccgc caagccttga ttgactattt gacaccgctc
      601 aaggagacct tgtctaagga tagccaacgt cgcttggagg aaaatcctct tcgtgtcttg
      661 gactctaagg aaaaagaaga caaggtggca gtagagaatg cgccgtctat cttggacttt
      721 cttgatgaag aaagccaagc tcattttgat gctgtgcgtc agatgttgga aaatcttgga
      781 gtagattaca tcatcgatac caatatggtg cgtggtctgg actactacaa ccacaccatt
      841 ttcgagttta tcacagagat tgagggcaat gacttgacag tctgtgcggg tggtcgctac
      901 gatggtttgg ttgcttactt tggaggccct gaaactgctg gatttggttt tgggcttggt
      961 gtagagcgcc tgcttctcat ccttgaaaaa caaggcgtgg ccctccctat cgaaaatgcc
     1021 ctagatgtct atatcgcagt cttgggtgat ggagcaaatg tcaaagccct agaactagtc
     1081 caagctcttc gccaacaagg tttcaaagca gagcgtgatt acctcaaccg taagctcaaa
     1141 gctcagttca agtcagccga tgtctttgcg gctaagaccc tcatcaccct aggagagagc
     1201 gaagtcgaaa gcagacaagt gacagtcaag aacaaccaaa cccgagaaga agtgcaagtg
     1261 tcacttgaga caatcagcca aaacttctca gaaatctttg aaaaattagg attttatact
     1321 caatgaaaat caaagagcaa actaggaagc tagccgcagg ttgctcaaaa cactgttttg
     1381 aggttgtaga tgaaactgac gaagtcagta accataccta cggcaaggtg aagctgacgt
     1441 ggtttgaaga gattttagaa gagtattaat gatagataaa gtggtcagga acttactcct
     1501 gacctttttc ctttgcaaaa tgacaaaaat cataaacttt ttgacaacta tacttgtcaa
     1561 aaagaaaaag atgtgttaca atgtaagcaa gttaagagaa aagaagaaag gaaacgcaga
     1621 aatgtcgaga ttattacaag aaatgattgg aaaggaacca attataacgg gagtgtatat
     1681 tgggccagat aactgggaag ttgtggatgt tgacgaggaa tgggtcaaac tacgtagtgt
     1741 tgataaaaag gggaaagaaa aattcaagtt gcaacgtatt gaagatatcc aaaccgttga
     1801 atttgacgga gagtaggtgt gtaacatgca actaaaaaat cgtctaaaag agcttcgagc
     1861 tcgcgatggt ctcaatcaaa ccgacctagc caaactggca ggggtttcca gacagaccat
     1921 tagcctacta gaacgggatg agtacacccc atccattatc attgccctga aaatatctca
     1981 aattttcaac gaaacagtcg aatcggtatt tcgtttggag gaggacgagt gatgaacaag
     2041 tataaagtga tttattatgt agtggtcata gctttattag tcagcgtatt tctactgatt
     2101 gggatggacc taagctggtt taatccctat caaagcgacc aatttgtttg ggtctacttt
     2161 gctctcatcc cagtaactga atggattgaa aagaaatcca aaaatctagc aagtgaaaaa
     2221 ggagaatgaa tatgaaaaag aaacgtagat tgttattttt gatgtctatt gtcttggggg
     2281 ggttcttggg catgtttgta gggatgttta aggcacgtgt cgaatcccac gaaattattt
     2341 tagatgtaaa agccttgatg ccatggatat cagctatttg tttactgata ggtttcatta
     2401 gtatgttttt gactttcaat ttcttaaaga aaagcagaaa atttcattcc ttgtatcaag
     2461 aggaaatgga tgacgatctg aatgaaacct attatgtgca aatgtatcgg aatcttgagt
     2521 ttggaaccat tgcttttaat attacaggtg tagcgattcc attggctatt tttatttcat
     2581 taagtgaggt gattatattg catacaaacc ctcaaacatt tttcctttct ttcttactct
     2641 ttgtggtatt cttagtcgct caaaaatctc tttttaaaac cattgcgatt gttcgtcagt
     2701 ttgatttgga atttttcgct acaccaaagg atgtcttgaa ctatataaat tcttatgatg
     2761 aaggggagcg tcaggctaat ttggaacaga gttttcgaat tttattccaa ttacaccaat
     2821 atgtcttacc agccttatat atttttctta ttatcatttc tttcttgaca ggagagattc
     2881 agttactagc tttcttgctt gtaggagcca tccatgttta tatcaatgtg atgcagttac
     2941 ctatggtaaa acgttatttc aaataaagga gttatctatg aaattactta aaaaccttgg
     3001 ctggattctt ctagcccttc tatccttttt atttatctat ggctttattc aggggctcgc
     3061 gactgcgtcg cttgctttag gcgcttcacc ctatgctgtt actctactct atgtggcctt
     3121 ggctggagta tatgtgtacg gcatttacaa atggtatcag aaggctcctg tttatattga
     3181 gaagagcggc tttaaccgat ttatttggct tcctgccttg gtttggttct tatctctggt
     3241 cgttcaattc ttcttgccag atgatccttc agtaaatcaa caaatagcga cagacttgac
     3301 cttgtctcaa ccacttttct cattctttgc tgtggttatt tttgctcctt tgacggaaga
     3361 gattgttttt agagggatgt tagcacgcta tctctttcct aagcaggaca atagtaaacg
     3421 aaccctgatt tttcttctgg tatctagtct tctatttgcc ttgattcatt ttccaggtga
     3481 tgtgcaacaa ttttttgtct attttagcct tggttttagt ttgggtttgg cttacattag
     3541 cagaaaaggt ctggtctaca gtatttctct tcacgctttg aataatttag tcggcttttt
     3601 gatgattctc atgctataat agagtcagga ggtcacatga aacgagtaat tttagtagca
     3661 gtgattcagg cagtcattct atttttcatc attggagcgc tagcttatgc cttcaaaggc
     3721 gatttcttct ataactatct agcagttgtc tttgctccta ttgcaggtgt actgcgtttt
     3781 gggacggctt acataacgga aattgtcttg cctcgaaagg cagccgaaat cgctgaaaag
     3841 cgtaaagcag gcaaaaattc aaaataaaag agtccggtga agttcatcgg atttttgtat
//
LOCUS       DAVMEI010000141         3886 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVMEI010000141 DAVMEI010000000
VERSION     DAVMEI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.141"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..579)
                     /locus_tag="VYQ24_001718"
     CDS             complement(<1..579)
                     /locus_tag="VYQ24_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1968827.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTE"
     gene            complement(879..3662)
                     /locus_tag="VYQ24_001719"
                     /pseudo
     CDS             complement(879..3662)
                     /locus_tag="VYQ24_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         1266 a          832 c          554 g         1234 t
ORIGIN      
        1 ctcagtacaa tagaaatctt gaatgatttt ctcaattccc ataattattt cagtgatttt
       61 atacgactct tgaaaattat tttctaaatt agcattaaca aaatgtagcg ctatgaatgc
      121 agcttcgtca attggaagtt caatacctaa ctcttttttt attatttgta aagccctttt
      181 cccaagttca aattcttctt tataatattt cgatatttcc cagcgtaggg gattcgaaat
      241 aataatccct tctttataac gttctatgct cgaagaaata tgatccgtta aattaatata
      301 gataatttca tcaaaattat tacccaatgt tattttacct aggttaatta ttttttcggt
      361 gatgtacacc aactccaaag gaacgttaat aaacatctct gtaaaacggg tcatattatc
      421 tctatttttg agaaaaaaac ttttctcaat atcaattgga tttactttat ctccagtttt
      481 tcttccaaaa gctattcccc ttccaaaaag aagaatatcg atatcttttt tactttgcgc
      541 aattacggca ttgtggttta gtattcgttt tatgatcata aaatttccct tttaaaataa
      601 aaggaaccta cttaaaaaca tcaagaaact tcttctgatg tgattaaata ggttcctgct
      661 cacttgtatt agtgataaca tccttttatt tcaattttta atggtgacat gaagcttaaa
      721 taattaataa ctctgtaacc gtttttattt tatgatattt caatctattt gtcaagtact
      781 tataaaaatt tttttatttt gtgtgattcg aatgtgataa tggcaacagt ctcactaaag
      841 aaatgaaaat catacataga aatttttaaa aattttctct atcgtaaacc tgccactagt
      901 tcagaaacta tactagctga ttccaaacgt aatatggtca tctcatctac cttccctaat
      961 aatggcattg caccatacca aacaatagtc ttattcacca aaagaatagg cgttactatt
     1021 tttgattgag taaaagacac tttaattttc tggctcttca actggtctag ccatgcttta
     1081 tttgcaaccg tctctggtat acatatctca atttgactaa tcctaaattc ttttagaaaa
     1141 ttcatcagtt ttgtctggtg cacataaggt aaaataagca gacattcctg tctttcccct
     1201 gctaagtcct ctctcaacac cttctcatat ctactatcaa cataaacgaa ttgtttctcc
     1261 tcaccctcga tgacacgata atccatcttt cgataagcta cttgtcgttt ctgaaacatc
     1321 ttttctaaat aaggaacatg aatatccaca taatcgaaaa tacgcaccaa agacttatcc
     1381 ttgtagtttc tatgaatccg acctgcatac tgaattaaat tatttttcca agaaaagggt
     1441 gctgccaaga taagcgtgtc caactgaggt aagtcaaaac cttcgccaat gtattttcca
     1501 gtagacaaca aaacaaaccc tttatctaac tgttctaacg tctccagtaa actcgttctt
     1561 tctcggactt tggtttttcc gctaataatg taacagccat caacctcttt ctctttcaat
     1621 aacttttcaa agacatctat ctgttgaatt cgattaacta aaaccaagat attccgtcct
     1681 tctgccactt gggctagaat atccttgaga atcaactgat tcctcgctga gtctgtagca
     1741 atccaatcac tgagctgtat aaaattactt gctttggtct tttcaatctc caaatgacca
     1801 aaagaagtga atcttaattg caattgccgt ttaaaatccg tttccctctt atcagcagta
     1861 tggagtatct caccaattct ctgaaaaaca ataggctcat gaccattctt acgctcaggc
     1921 gtagccgtca aaccgtaaag atacttccct ctaaactgag caacaacttt ttcaaacatc
     1981 aaggcagaga catgatgaca ctcatccaca atcatcatct catactcatc caaaagactt
     2041 tgactatttt ccaacttaaa tagagattga atcataacga catcaaccag tttactcagc
     2101 catttcttag tcccaccgta ctgtccaaca tagccgatta ccttttcacg acctgatgcc
     2161 gtataacgga tagcctcctc ctcttcgaaa gtcaaaaagc agtttaagcg atctagccat
     2221 tggtctaaga gttgcttatt atggactaga ataattgttt ttgttttccg ttcagagata
     2281 agagcagcac ctaaaacggt cttcccaaaa ccagtctccg catgaagtaa accattttct
     2341 ttagaattca tatctgacag ggctaattct tgctccaaag taagctctcc cttaaatgct
     2401 actctaatag acctttgtac ttttctccta tcctccacaa ctacctgctt aaatttatct
     2461 tgcaatggat ataacaagcc tcttggcagc cataaataat aatcggattc tccaaataaa
     2521 tacattcgct caggaatttg ataggttggc tgtcgcatag cctgctttaa atatagtaaa
     2581 atgaaataat aacaggacaa atcgatcagg aaagtcaaat cgatttctaa caatgtttta
     2641 gaagcagagg tgtactattc tagtttcaat ccactataaa attcgggatt agaaaaggaa
     2701 gccatatttt tcaagaaaaa aagtgccttg gatgtcacag acgatttttc aagttggatc
     2761 atattggata aaacgacctt caactcctta tccaattctt gcttgcctaa ctcctcttgg
     2821 attaacagtg ccactttagc agttgaaatc ctctgaattt cttgtagata cctccattga
     2881 tcttcataag gctgaaaatg ttcatccaca aagaccgttc gcccttgatg ataagcttct
     2941 ccttgaaaag gaagggaaat taaattatca aatccacctt taggaagaac atcctgattt
     3001 ggaaacatgc catcaaaaga atcaaaggac agttgcatac tttcctgcat agccaattct
     3061 aatagtttct ttccaaacag gcgagccttt cgactcagaa tcgcttcctc aaagaagaac
     3121 caaatatgga gtccgtaacc cgaacgagaa atctctaaat gggcttccat ctggcgttcc
     3181 ctggctattc ttcgaatggt taaaccagct tctttccaat ctccttcatc aaaatccagt
     3241 accaaaaaat gacagctatc atctaagtgc ataggaaaga taccgatagc tgcctcccca
     3301 cgaaaatgag atttcaaaac ggagttcgtc aatgtctgga aactccgttt ttcaggtggc
     3361 aactgcttcc aaccataatc ataggaagga aaatactgaa ttttcccttg ttcattgata
     3421 aaattcttag cataaacatc ataacgtcca gcaaacacgg aggtaaacaa ttgtaatttt
     3481 tcttgcgtcg tcaagcgact actttgaagt tggaacatct cctcgaactg agagcgttcc
     3541 ataacaaact gactcttgtc tttaagacgt ttacacaaga aaaactcact atccccatta
     3601 aaagaagaaa agacatcaag tacttctacc acaattccat ccaaagacac aaaaacagcc
     3661 ataagagtca cctccttgat tcctataggc tgattataac aagaatagct gaaattgtac
     3721 acgaaaataa aatcctaata gtactcattt tgtatgtggc taatattccg tctcgctccg
     3781 gaatgtacga ggtaaataga gtttaaacga gcagtctata aaattcaaaa aacgcatcat
     3841 atcaggtgta catgaactac accccaaaag ttagacagaa aaaatc
//
LOCUS       DAVMEI010000142         3854 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVMEI010000142 DAVMEI010000000
VERSION     DAVMEI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.142"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            7..273
                     /locus_tag="VYQ24_001720"
     CDS             7..273
                     /locus_tag="VYQ24_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1968828.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            266..634
                     /locus_tag="VYQ24_001721"
     CDS             266..634
                     /locus_tag="VYQ24_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1968829.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            754..2022
                     /locus_tag="VYQ24_001722"
     CDS             754..2022
                     /locus_tag="VYQ24_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1968830.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFSIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2019..3296
                     /gene="tilS"
                     /locus_tag="VYQ24_001723"
     CDS             2019..3296
                     /gene="tilS"
                     /locus_tag="VYQ24_001723"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1968831.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVENLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3300..3842
                     /gene="hpt"
                     /locus_tag="VYQ24_001724"
     CDS             3300..3842
                     /gene="hpt"
                     /locus_tag="VYQ24_001724"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1968832.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
BASE COUNT         1389 a          543 c          747 g         1175 t
ORIGIN      
        1 ataaggatga gattagataa atatttaaaa gtatcgcgaa ttatcaagcg tcgtacagtc
       61 gcaaaggaag tagcagataa aggtagaatc aaggttaatg gaatcttggc caaaagttca
      121 acggacttga aagttaatga ccaagttgaa attcgctttg gcaataagtt gctgcttgta
      181 aaagtactag agatgaaaga tagtacaaaa aaagaagatg cagcaggaat gtatgaaatt
      241 atcagtgaaa cacgggtaga agaaaatgtc taaaaatatt gtacaattga ataattcttt
      301 tattcaaaat gaataccaac gtcgtcgcta cctgatgaaa gaacgacaaa aacggaatcg
      361 ttttatggga ggggtattga ttttgattat gctattattt atcttgccaa cttttaattt
      421 agcgcagagt tatcagcaat tactccaaag acgtcagcaa ttagcagact tgcaaactca
      481 gtatcaaact ttgagtgatg aaaaggataa ggagacagca tttgctacca agttgaaaga
      541 tgaagattat gctgctaaat atacacgagc gaagtactat tattctaagt cgagggaaaa
      601 agtttatacg attcctgact tgcttcaaag gtgataaaat ggaaaattta ttagacgtaa
      661 tagagcaatt tttgagtttg tcagatgaaa agctggaaga attggctgat aaaaatcaat
      721 tattgcgttt acaagaagaa aaggaaagga agaatgcgta aattcttaat tattttgttg
      781 ctaccaagtt ttttgaccat ttcaaaagtc gttagcacag aaaaagaagt cgtctatact
      841 tcgaaagaaa tttattacct ttcacaatct gactttagta tttattttag agaaaaatta
      901 agttctccca tggtttatgg agaggttcct gtttatgcga atgaagattt agtagtggaa
      961 tctgggaaat tgactcccaa aacaagtttt caaataaccg agtggcgctt aaataaacaa
     1021 ggaattccag tatttaagct atcaaatcat caatttatag ctgcggacaa acgattttta
     1081 tatgatcaat cagaggtaac tccaacaata aaaaaagcat ggttagaatc tgactttaaa
     1141 ctgtacaata gtccttatga tttaaaagaa gtgaaatcat ccttatcagc ttattcgcaa
     1201 gtatcaatcg acaagaccat gtttgtagaa ggaagagaat ttctacatat tgatcaggct
     1261 ggatgggtag ctaaagaatc aacttctgaa gaagataatc ggatgagtaa agttcaagaa
     1321 atgttatctg aaaaatatca gaaagattct ttctctattt atgttaagca actgactact
     1381 ggaaaagaag ctggtatcaa tcaagatgaa aagatgtatg cagccagcgt tttgaaactc
     1441 tcctatctct attatacgca agaaaaaata aatgagggtc tttatcagtt agatacgact
     1501 gtaaaatacg tatctgcagt caatgatttt ccaggttctt ataaaccaga gggaagtggt
     1561 agtcttccta aaaaagaaga taataaagaa tattctttaa aggatttaat tacgaaagta
     1621 tcaaaagaat ctgataatgt agctcataat ctattgggat attacatttc aaaccaatct
     1681 gatgccacat tcaaatccaa gatgtctgcc attatgggag atgattggga tccaaaagaa
     1741 aaattgattt cttctaagat ggccgggaag tttatggaag ctatttataa tcaaaatgga
     1801 tttgtgctag agtctttgac taaaacagat tttgatagtc agcgaattgc caaaggtgtt
     1861 tctgttaaag tagctcataa aattggagat gcggatgaat ttaagcatga tacgggtgtt
     1921 gtctatgcag attctccatt tattctttct attttcacta agaattctga ttatgatacg
     1981 atttctaaga tagccaagga tgtttatgag gttctaaaat gagggaacca gattttttaa
     2041 atcattttct caagaaggga tatttcaaaa agcatgctaa ggcggttcta gctctttctg
     2101 gtggattaga ttccatgttt ctatttaagg tattgtctac ttatcaaaaa gagttagaga
     2161 ttgaattgat tctagctcat gtgaatcata agcagagaat tgaatcagat tgggaagaaa
     2221 aggaattaag gaagttggct gctgaagcag agcttcctat ttatatcagc aatttttcag
     2281 gagaattttc agaagcgcgt gcacgaaatt ttcgttatga tttttttcaa gaggtcatga
     2341 aaaagacagg tgcgacagct ttagtcactg cccaccatgc tgatgatcag gtggaaacga
     2401 ttttgatgcg cttgattcga ggaactcgct tgcgctatct atcaggaatt aaggagaagc
     2461 aagtagtcgg agagatagaa atcattcgtc ccttcttgca ttttcagaaa aaagactttc
     2521 catcaatttt tcactttgaa gatacatcaa atcaggagaa tcattatttt cgaaatcgta
     2581 ttcgaaattc ttacttacca gaattggaaa aagaaaatcc tcgatttagg gatgcaatct
     2641 taggcatcgg caatgaaatt ttagattatg atttggcaat agctgaatta tctaacaata
     2701 ttaatgtgga aaatttacag cagttatttt cttactctga gtctacacaa agagttttac
     2761 ttcaaactta tctgaatcgt tttccagatt tgaatcttac aaaagctcag tttgctgaag
     2821 ttcagcagat tttaaaatct aaaagccagt atcgtcatcc gattaaaaat ggctatgaat
     2881 tgataaaaga gtaccaacag tttcagattt gtaaaatcag tccgcaggct gatgaagagg
     2941 aagatgaatt tgtgttacac tatcaaaatc aggtagctta tcaaggatat ttattttcct
     3001 ttggacttcc attagaaggt gaattaattc aacaaatacc tgtttcacgt gaaacatcca
     3061 tacacattcg tcatcgaaaa acaggagatg ttttgattca aaatgggcat agaaaaaaac
     3121 tcagacgttt atttattgat ttgaaaatcc ctatggaaaa gagaaactct gctcttatta
     3181 ttgagcaatt tggtgaaatt gtctcaattt tgggaattgc gaccaataat ttgagtaaaa
     3241 aaacgaaaaa tgatataatg aacactgtac tttatataga aaaaatagat aggtaaaaaa
     3301 tgttagaaaa cgatattaaa aaagtcctcg tttcacacga tgaaattaca gaagcagcta
     3361 aaaaactagg tgctcaatta actaaagact atgcaggaaa aaatccaatc ttagttggga
     3421 ttttaaaagg atctattcct tttatggctg aattggtcaa acatattgat acacatattg
     3481 aaatggactt catgatggtt tctagctacc atggtggaac agcaagtagt ggtgttatca
     3541 atattaaaca agatgtgact caagatatca aaggaagaca tgttctattt gtagaagata
     3601 tcattgatac aggtcaaact ttgaagaatt tgcgagatat gtttaaagaa agagaagcag
     3661 cttctgttaa aattgcaacc ttgttggata aaccagaagg acgtgttgta gaaattgagg
     3721 cagactatac ctgctttact atcccaaatg agtttgtagt aggttatggt ttagactaca
     3781 aagaaaatta tcgtaatctt ccttatattg gagtattgaa agaggaagtg tattcaaatt
     3841 agaaagaata atct
//
LOCUS       DAVMEI010000143         3837 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVMEI010000143 DAVMEI010000000
VERSION     DAVMEI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.143"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            59..913
                     /locus_tag="VYQ24_001725"
     CDS             59..913
                     /locus_tag="VYQ24_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1968833.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EHEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            1025..1909
                     /locus_tag="VYQ24_001726"
     CDS             1025..1909
                     /locus_tag="VYQ24_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1968834.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            2072..3811
                     /locus_tag="VYQ24_001727"
     CDS             2072..3811
                     /locus_tag="VYQ24_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEV1968835.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHI
                     QQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWR
                     LDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIK
                     DYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSA
                     LAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIWKYA
                     SVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELE
                     NQLVISPDGFVIF"
BASE COUNT         1233 a          629 c          826 g         1149 t
ORIGIN      
        1 aaataaatat ggtatataac caatataaaa agcatcaaaa tgaacaagga ggaatctgat
       61 gcttggagca attattggag atattgtcgg ttctgtttac gaatggaaca atatcaaaac
      121 aaaggacttt cctttatttc ggaaggactg cttttttaca gatgacacgg ttatgacctg
      181 tgctgttgca gaagcaatta tgaatggtgg acaaaaagac gatttcattg acgcgatgaa
      241 gaaatatggc agaatgtatc cgaatgctga ttacggtgct cggtttaatg catggctaaa
      301 cagcgataac cgtgagcctt ataatagctt tgggaatgga tcagctatgc gtatttctcc
      361 atgtgcttgg atcatggact gtggttttta tgcgaaaact ggtatgtggc catcatctag
      421 aggacttacg agtctttctg cagaggtaac tcataatcat ccagaaggtg tcaaaggcgc
      481 tatggctaca gctgatgcta tctttctatg tcgtttttac tttggtggtt attgtagaga
      541 gcacgagcaa tcaattaacg acaatcctac agagtgtaaa agacgaatta aggattatat
      601 agaaaaagaa tacgattaca atctatctca aactttagat gaaatccgtc ctaactatcg
      661 ttttaacgaa acatgtcagg aaactgtccc tcaagccatt atcgcctttc ttgaaagtag
      721 agacttcgaa gatgcgataa gaaatgccat ctcacttggt ggcgacagtg atactctcgc
      781 agcaatcact tgtagcatag cagaggcagc ttacggtatt cctgattgga tcaaggataa
      841 ggcctattct tacttggatg aacccttaaa ggatgtagtt aggcgatggg aaaatagaat
      901 aaaagcgtat taatctccag tacgtcagat ttctcttttc tgtattttca ttgtataata
      961 ggattacatt tacttgcata cgacgaaatg taaacatttc tgtttaaaat taaggataaa
     1021 atcaatgaaa aaagcaatgg taattatcaa ccctacttct ggtggcgaga aggctttgga
     1081 ttacaaagaa aagctggaga ataaagcaaa agaatacttt gaatatgttg aaaccaaaat
     1141 taccgaaaaa gcgctggatg caacacattt tgctgaagaa gcttctcgtg agcagtatga
     1201 tgcagtggtt gtgtttggtg gagatggaac tgtcaatgaa gtcatttcag gtattgatga
     1261 gagagactac attcctaagt tagggattat cccaggcggt acgggtaacc tcattacaaa
     1321 acttttggaa atcaatcaag acatcgatgg cgcaattgac gaactggatt ttgatttaac
     1381 caataagatt gatatcggta aagcaaatga caactatttt ggttatatct ttagtatcgg
     1441 ttctctgcct gaggcgattc acaatgttga aatagaggac aaaacaaaat tcggtatttt
     1501 aacctatgct gtaaatacca tgaagtctgt catgacagat caggtcttta acattaaggt
     1561 tgagacagaa aatggaaatt atgttggtga agctagccat gttttggtcc ttttgacaaa
     1621 ttacttcgct gataagaaaa tctttgaaga aaacaaggac ggctatgcca acattttgat
     1681 tctgaaagat gcctctatat tctccaaatt atccgtcatt cctgatttat taaaagggga
     1741 tgttgtcgca aatgataata tcgagtatat caaagcgcgt aatattaaaa tctcttcaga
     1801 tagtgaattg gagtcagatg ttgacggaga taaatcagat aacctacctg tagaaatcaa
     1861 agtcctagct cagcgagtag aagtattttc aaaaccgaaa gaggattagt atatagagaa
     1921 agcctttttt aaggcttttt gtatacttta aaagatagtt cctttaacaa cggacattcc
     1981 ttgcaaatag tttacaaaaa tagtatactg gatttattga gtttgaaaac gtttgcgtaa
     2041 aatttgaatg aatactttag gagacaaatt gatggaattg agtgctattt accataggcc
     2101 tgagtcggag tatgcctatc tttataagga taagaaactc catattcgaa ttcgaactaa
     2161 gaaaggggac attgaaagca tcaacttgca ctatggggac ccttttatct ttatggagga
     2221 gttttatcag gatacaaaag aaatggtcaa gataacttct ggtaccttat ttgaccattg
     2281 gcaggttgaa gtgtcagttg actttgcacg tatccagtat ctctttgagc tcagagatac
     2341 agaaggtcaa aatattttgt atggcgataa agggtgtgtg gaaaattctc tagaaaatct
     2401 tcatgcaatt gggaatggat ttaagttgcc ttatcttcat gagattgatg cctgcaaggt
     2461 tcctgactgg gtttcaaata cggtatggta tcagatattt cctgaaagat ttgccaatgg
     2521 caatgctcta ttaaacccag aagggacttt agactgggat tcatctgtca cacctaagag
     2581 cgatgatttc tttggtggtg atttacaggg gattattgat catatggatt acttgcaaga
     2641 cttgggtatt actggactat atctttgtcc catctttgaa tctacaagca atcacaagta
     2701 caatacgaca gattactttg aaattgaccg tcattttgga gacaaggaga cctttcggga
     2761 actggtggat caagcgcatc atcgtggcat gaaagtcatg ctggatgcgg tatttaatca
     2821 tattggttcg caatctcttc aatggaaaaa tgtcgtcaaa aatggtgaac agtctgctta
     2881 taaggattgg ttccatattc aacaattccc agtgacaact gaaaagctag ttaataagag
     2941 agacttaccc tatcatgttt ttggtttcga ggactatatg cctaagctaa atacagccaa
     3001 tccagaggtc aagaattatc ttttaaaggt tgcgacttat tggattgaag agtttaatat
     3061 cgatgcttgg cgtttggatg tggctaatga gattgaccat cagttctgga aggattttcg
     3121 taaggcagtt ttagctaaaa atcctgatct ttatatccta ggagaagtct ggcatacatc
     3181 tcagccttgg ctaaatggag atgagttcca tgccgtcatg aattatcctt tatctgatag
     3241 tatcaaggac tatttcttac gaggaattaa gaagacagac cagttcatcg atgaaatcaa
     3301 tggagagtct atgtattaca agcagcagat ttcagaggtc atgtttaatc tcttggattc
     3361 acatgataca gagcgaatcc tgtggacggc caatgaagat gttcaactgg ttaaatcagc
     3421 cttagccttt ctctttttac aaaaaggaac accgtgcatt tattacggaa ccgagctagc
     3481 cttgactgga ggaccagatc cagattgtcg tcgttgtatg ccttgggaac gtgtatcaag
     3541 tgacaatgat atgctgaact ttatgaagag gctgattaag atttggaaat acgcgtcagt
     3601 aatcatttcg catggcaagt atagccttca agaaatcaaa tctgatctag tagctctgga
     3661 atggaaatac gaaggacgga tcctcaaagc aatattcaac caatcaacag aagattatct
     3721 tttagagaaa gaagcagtag cactagcaag caattgccaa gaattggaga atcagcttgt
     3781 catctctcca gatggatttg tgattttcta aaaactagtt gatgaagatt atggtac
//
LOCUS       DAVMEI010000144         3830 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVMEI010000144 DAVMEI010000000
VERSION     DAVMEI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.144"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            70..1329
                     /locus_tag="VYQ24_001728"
     CDS             70..1329
                     /locus_tag="VYQ24_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1968836.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGAASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1405..1540
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1579..2079
                     /locus_tag="VYQ24_001729"
     CDS             1579..2079
                     /locus_tag="VYQ24_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1968837.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2155..2523
                     /gene="rplL"
                     /locus_tag="VYQ24_001730"
     CDS             2155..2523
                     /gene="rplL"
                     /locus_tag="VYQ24_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1968838.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2710..2880
                     /locus_tag="VYQ24_001731"
     CDS             2710..2880
                     /locus_tag="VYQ24_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968839.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2880..3128
                     /locus_tag="VYQ24_001732"
                     /pseudo
     CDS             2880..3128
                     /locus_tag="VYQ24_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3142..3482
                     /locus_tag="VYQ24_001733"
                     /pseudo
     CDS             3142..3482
                     /locus_tag="VYQ24_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3475..>3830
                     /locus_tag="VYQ24_001734"
     CDS             3475..>3830
                     /locus_tag="VYQ24_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968840.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMA"
BASE COUNT         1219 a          681 c          819 g         1111 t
ORIGIN      
        1 acctacggca aagtgaatct gaagtggttt gaagagagta caacttgtct tttagaaaag
       61 gagcctataa tgaaagtctt tcagcatgta aatatcgtga cttgtgatca agatttccat
      121 gtttatcttg atggaatctt agcagtcaag gattctcaaa tcgtctatgt cggtcaagat
      181 aagccagcgt ttttagagca agctgagcag attatagact atcagggagc ttggattatg
      241 cctggtttgg tcaattgtca cacccattct gcaatgacag gtctgagagg gatccgagat
      301 gacagcaatc tccatgaatg gctcaatgac tatatttggc cagcagaatc tgagtttact
      361 cccgacatga ctaccaatgc ggtcaaagaa gccctgacag agatgctcca gtcaggaaca
      421 acaaccttta acgatatgta taatcccaat ggtgtggata tccagcaaat ttatcaggtg
      481 gtgaaaactt ccaagatgcg ttgttatttt tctccgactc tcttttcttc agagacagaa
      541 acaactgctg agactataag tagaactcga tccatcatag acgaaatctt aaaatataaa
      601 aatccaaatt tcaaggttat ggtagcacct cattctccgt atagctgcag tagagacttg
      661 ctggaagcga gtttggaaat ggcaaaagag ctaaatattc cgctccatgt ccatgtggcg
      721 gagaccaagg aggagtcagg aattatcctc aaacgatacg gcaaacgccc ccttgctttt
      781 cttgaagaac tgggttattt agatcatccg tccgtatttg ctcacggggt cgaattaaac
      841 gagagagaaa ttgaacgctt ggcatcttct caagtggcta tcgcccacaa tcctatcagt
      901 aacctcaaac tggcatcagg aattgctcca attatccagc tccaaaaagc gggagtagca
      961 gtcggaattg cgactgactc ggttgcttcc aataacaatc tagatatgtt tgaggaagga
     1021 aggactgcag ctcttcttca gaagatgaaa agtggggctg ccagccagtt tccaatcgaa
     1081 acagctctca aggtactgac aatcgaaggg gctaaggtcc ttggaatgga aaatcagata
     1141 ggaagtctgg aagtcggcaa gcaagcagat tttctggtca ttcaaccaca agggaaaatt
     1201 catctccaac ctcaggaaaa tatgctgtct cacctggttt atgcagttaa atctagtgat
     1261 gtagatgatg tttatatcgc cggagaacag gttgttaagc aaggtcaagt cctgacagta
     1321 gaactttaaa agaaaaatta cgaaaaattt taaaaaaagt ttgcaaaaat cttgcattct
     1381 ttttttgact atgctatact tatatacggt ttaaaaaaac tgcctaagac agtaggggag
     1441 ctcgactcat aaatatccta ccgaggacaa aacgtatcat gtaaaaagaa gcgtattgta
     1501 ctttcgtgtc taggtttggg cgcgtttttc tttttgaaaa attccccaag caaaataatt
     1561 acggaggtga acacactaat gagtgaagca attattgcta aaaaagcgga actagttgac
     1621 gtagtagctg aaaaaatgaa agctgctgca tctatcgtcg ttgtagacgc tcgtggtttg
     1681 acagttgagc aagatacagt tcttcgtcgt gagcttcgtg gaagcgaagt tgagtataaa
     1741 gttattaaaa actcaatctt gcgtcgtgca gctgaaaaag ctggtcttga agatcttgca
     1801 tctgtatttg ttggaccatc tgcagtagca ttttctaatg aagatgttat cgcaccagcg
     1861 aaaatcttga acgacttttc taaaaacgct gaagcacttg aaattaaagg tggtgcaatc
     1921 gaaggcgctg tcgcatctaa agaagagatt cttgcacttg caactcttcc aaaccgcgaa
     1981 ggacttcttt ctatgctcct ttctgtactt caagcgccag tgcgcaacgt tgctcttgca
     2041 gtcaaagcgg ttgcagaaag caaagaagac gcggcttaat cttaagctac acagcgtagc
     2101 ctagctacga aaaaaactat tataaaattt aaaacttatt tggaggaaat aacaatggca
     2161 ttgaacattg aaaacattat tgctgaaatt aaagaagctt caatccttga attgaacgac
     2221 cttgtaaaag ctatcgaaga agaatttggt gtaactgcag ctgctcctgt agctgttgct
     2281 gcagctgatg cagctgatgc tggtgctgct aaagattcat tcgacgttga attgacatct
     2341 gcaggcgaca aaaaagttgg cgttatcaaa gttgtacgtg aaatcactgg tcttggtctt
     2401 aaagaagcta aagaacttgt tgacggtgca ccagcacttg ttaaagaagg cgttgcaact
     2461 gcagaagctg aagaaatcaa agctaaattg gaagaagctg gagcttcagt tactcttaaa
     2521 taatagagta tcgcaatttg attgcggaat ccctatttac cagtctttga gagcgataag
     2581 cgattgatag aaactgataa attgatttgg tttcctgcca aaaattcttt ggcaggattt
     2641 ttgttttaat aaactttcgg gggtagacac tagattgtct accccttttt ttgtgtttcg
     2701 gaggtaatta tgcaaaagga aggacagtca tcacgtcaat tcgtgaagag tcatctcatg
     2761 actatcatgg gagttgatat tgtaaaagca actcagttag ttgatgaaat ggaacaagta
     2821 ggtttaatcc gatttgatga atttggaaat gttggaattt tagtcttgga gggacaataa
     2881 tgaagcgtat taccgctaat caatatcaga catcagagcg ttattataag ctccccaaac
     2941 ttttatttga gagtgaacgg tataagaata tgaaactgga ggttaaggtg gtttactcag
     3001 ttttaaaaga tcggttggag ttgtctttga gcaaaggttg gattgatgag gatggggcta
     3061 tttatttgat ttattccaat tcaaatttga tggcactttt aggctgttca aagtcaaaat
     3121 tactctccat gtgagtttga agtgacattt ttagatgatt accataaaaa acataactac
     3181 ccactatttt acgaatccta tcttcaaaac gttatggaat tccttgaaag tcaagacata
     3241 aagaatgggg ttgatgcctt tgtagatgat catcaaaatc tcgtttttgt tttatatgga
     3301 caaggctatc gagccgaggg aaaagaggga atacttacaa cccaagtaac tgtaaaagct
     3361 tatgatgaag acaagaaacc gattaacttc gcaaatttat tagattcctt aatttattag
     3421 attccttaat cgtgtcagaa tatcaaatgg aaccgaatct ttgggaggtc tcccatgatt
     3481 gatctctatc taagtaaaaa tagacaaaga aatcaacttc ttttagattt cttccaaaac
     3541 tatggcatag aggtatcttg tcattcagtt tctgaaatga caaaggacaa attaattgag
     3601 atgatgagct attcttcaga ttgttttgag tttttatctc ccaatttatt acgatttaag
     3661 aaccgtgaca atctaagatt aacggatttc gttgagataa ttttaaaaaa tcctgaacta
     3721 accatcagac ttcctcttgc ggtttcaaat aagcgagttt atccaagtct gaatttggaa
     3781 gaggctagag ctctattgcc aagagatacg aaacaattga tttacatggc
//
LOCUS       DAVMEI010000145         3823 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVMEI010000145 DAVMEI010000000
VERSION     DAVMEI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.145"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            38..1813
                     /locus_tag="VYQ24_001735"
     CDS             38..1813
                     /locus_tag="VYQ24_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV1968841.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            1814..3199
                     /locus_tag="VYQ24_001736"
     CDS             1814..3199
                     /locus_tag="VYQ24_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV1968842.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            3222..>3823
                     /locus_tag="VYQ24_001737"
     CDS             3222..>3823
                     /locus_tag="VYQ24_001737"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV1968843.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIM"
BASE COUNT         1232 a          567 c          848 g         1176 t
ORIGIN      
        1 tattctataa tttttggact tagtaaggag aataactttg actcaaggga agattataaa
       61 agtatcggga cctctagtta ttgcatcagg tatgcaggag gctaatattc aagatatttg
      121 ccgtgtaggt aagctagggt taatcggtga aattattgaa atgagaagag atcaggcatc
      181 tatccaagtc tatgaagaaa catctggtct tggtccggga gaacctgttg ttacaactgg
      241 agaacctctc tcggttgaat tagggccagg attgatttct caaatgtttg atggcataca
      301 acgcccatta gatcgattta aattggctac tcataatgat tttctagttc gtggggtaga
      361 agttccaagt ttggatagag atattaagtg gcattttgat tccactatag caattggtca
      421 aaaagtgagt acgggtgata ttcttggaac tgtcaaggaa accgaggtag ttaatcataa
      481 aattatggtt ccttatggag tatctggaga agtcgtttct attgcatctg gcgattttac
      541 aattgatgaa gttgtatatg aaataaaaaa attggacggt agtttctata aaggaacgct
      601 tatgcaaaaa tggcctgtcc gcaaggcgcg tcctgtttct aaacgtttaa ttccagaaga
      661 accattaatc acaggtcaac gagttattga tgcattcttt ccagtaacca aagggggagc
      721 tgcagcagtt cctggaccgt ttggagcagg aaagacagtt gtacaacacc aagtagctaa
      781 atttgccaat gttgatattg ttatttatgt cggttgtgga gaacgtggaa atgaaatgac
      841 ggatgtactg aatgagtttc ctgagttgat tgaccctaat accggacaat caattatgca
      901 acggacagtt ctgattgcta atacttcaaa tatgcctgtt gctgctcgtg aggcttcaat
      961 ttatacagga attaccatgg ctgagtattt tcgtgatatg ggctactctg tcgccattat
     1021 ggctgattca acttcacgtt gggcagaagc gctacgtgaa atgtcaggac gtctagaaga
     1081 aatgcctggt gatgagggtt atcctgctta tctgggaagt cgtatcgctg aatattatga
     1141 aagagcagga cgttctcagg ttctagggct tccagaacgt gaaggaacga ttactgctat
     1201 tggagctgta tcgccacctg gtggagatat ttcagaacca gttactcaaa acactttacg
     1261 gattgtgaaa gttttttggg ggcttgatgc tccgttggca cagcgacgtc attttcctgc
     1321 aattaactgg cttacatctt attcactata taaagacagt gtgggcactt atatagatgg
     1381 taaagagaag acagattgga atagtaaaat aactcgtgcg atgaactact tacaacggga
     1441 atctagttta gaggaaattg ttcgtcttgt tggaattgat tctctgtctg ataatgaacg
     1501 actaacgatg gaaattgcta aacaaattcg agaagattat ttgcaacaga acgcttttga
     1561 ttcggtagat acattcactt cgtttgcaaa acaagaagca atgctaagta atattctcac
     1621 ttttgctgat caggcaaatc atgctttaga gttgggttct tactttacag agattatgga
     1681 aggtaccgtg gcagttcgag accgtatggc gagaagtaaa tatgtttcag aagatagatt
     1741 agatgaaatc aaaattatat caaatgagat tacacatcaa attcatttga tattagaaac
     1801 aggaggtcta taaatgagtg ttataaaaga atacagaact gctagtgaag ttgttgggcc
     1861 tcttatgatt gttgaacaag taaataatgt gtcttacaat gagttagttg aaattcaact
     1921 tcataatgga gaaattcgtc gtggacaagt tttagagatc cacgaagata aagcaatggt
     1981 tcagcttttt gaaggatcta gtggaataaa tttagaaaag tctaaaattc gttttgctgg
     2041 tcatgcatta gaattggctg tatctgagga tatggttggt cgtattttta atgggatggg
     2101 aaaaccaatt gatggtggac cagatttaat tccagagaaa tatttagata ttgatggtca
     2161 agctattaat cctgtatcta gagattatcc agatgaattt attcagacag ggatctcctc
     2221 tattgatcat ttgaatactc ttgtacgtgg tcaaaaatta ccagtatttt caggttcggg
     2281 cttacctcat aatgaattag ctgctcagat agcaagacaa gcgactgttt taaattctga
     2341 tgaaaatttt gcggttgtat ttgcagcaat gggtattact tttgaagaag ctgagttttt
     2401 tatggaagaa ctcagaaaaa caggagcgat cgatcgttcg gttttattta tgaacttggc
     2461 aaatgatcct gcaattgagc gtattgcaac tccccgcatt gctttaactg cggcagagta
     2521 tctagctttt gaaaaagata tgcacgttct agttatcatg acggatatga ctaactattg
     2581 tgaagcgtta cgtgaagtct cggcagctcg ccgtgaagtt ccagggagac gaggctatcc
     2641 gggatattta tatacaaatt tatcaactct atacgaaagg gctggtcgct tagttggtaa
     2701 aaaaggttcg gtgacacaga ttcctatttt aacaatgcca gaagatgaca taacacatcc
     2761 aattcctgat ttaactggat acattactga agggcaaatt attttgtcgc atgagttgta
     2821 taatcaaggt tatcgtccac caatcaatgt tttaccttct ctctctcgat taaaagataa
     2881 gggatctgga gaaggtaaaa ctcgtggaga tcatgctcca actatgaatc aactgtttgc
     2941 agcctatgcc caagggaaaa aggttgaaga gttagcagta gtattaggag aatcggcttt
     3001 atctgatgta gataaattgt atgtgaggtt tacaaagcgt tttgaagaag agtacataaa
     3061 ccaaggattt tataaaaatc gaaatataga agatacgttg aatcttgggt gggaattact
     3121 atcaattctt cctagaacag agttaaaacg tatcaaagat gatttgcttg ataaatactt
     3181 acctttggta gaagtttaat ccggaaatgg agtgattatc tatggtacgt ttgaatgtaa
     3241 aaccaactcg tatggaattg aataacttaa aggaacgttt gacaacagct gaacgtggac
     3301 ataagttatt aaaggataaa agagatgaat tgatgaggcg atttatttct ttgattcgtg
     3361 agaataatca acttcggaaa gaagtggaaa gttatctaat tgataatcta aaatcctttg
     3421 cagttgctaa atcattaaag aattctcaaa tggtggagga attattttca attccatcga
     3481 aagaaattga attatttgtt gagaaagaaa atatcatgag tgtaacagtt cctagaatgc
     3541 atatgaatat tacttctcaa aatgagaaca gtgaatacag ctatttatct tctaatagtg
     3601 aaatggatga tgtatttgct acaatgaata gtttaattta taaattacta agactggcag
     3661 aagttgaaaa aacgtgtcag ttaatggctg atgaaataga aaaaacacgt agacgtgtaa
     3721 atggtttaga atactcgatt attccaaact tgtcggaaac tattcattat atagaattga
     3781 aactagagga ggcagaaaga gccaatttag ttcgtattat gaa
//
LOCUS       DAVMEI010000146         3815 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVMEI010000146 DAVMEI010000000
VERSION     DAVMEI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.146"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            219..887
                     /locus_tag="VYQ24_001738"
     CDS             219..887
                     /locus_tag="VYQ24_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1968844.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LSQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGDSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIDLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            847..1524
                     /locus_tag="VYQ24_001739"
     CDS             847..1524
                     /locus_tag="VYQ24_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1968845.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            1525..2283
                     /locus_tag="VYQ24_001740"
     CDS             1525..2283
                     /locus_tag="VYQ24_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1968846.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDIFLDGQSIVENKKNFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKTQGRDKKEVTEEALQLLDRVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADRIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            2295..3156
                     /locus_tag="VYQ24_001741"
                     /pseudo
     CDS             2295..3156
                     /locus_tag="VYQ24_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(3236..3790)
                     /locus_tag="VYQ24_001742"
     CDS             complement(3236..3790)
                     /locus_tag="VYQ24_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968847.1"
                     /translation="MIGITGANGSGKSVLFKLIAGLYSPSYGEVLINGENIVPERKIP
                     ANLGALIEEPGFINYYSGFKNLQYLASIRGVVGNQEISDTLKIVGLYEQKDQKVKTYS
                     LGMRKKLGIAQAIMENPSILLLDEPMNALDKSSVENMRTLFRKLSSEKGTTILIASHS
                     EEDIRILCDKVYAIEDKVCTLCSD"
BASE COUNT         1114 a          837 c          692 g         1172 t
ORIGIN      
        1 cctccttaat aagtaagcgt ttactttgtg tattacaaaa atatcttaac tctttttcaa
       61 aacttttaaa atattttgtt tggaaatttc agaaatttta tgtctatgat aaaaatcctt
      121 ataacggcaa taaaaaatag atattatcca aagaagattt taagtgctac aataactgta
      181 ttatttctag atgggaggtt ctatttttgg attgatccat tgttgaacaa tatctaccac
      241 tatatcaaaa ggcattcttt ctgaccttgc atattgcagt ttggggaatt ttgggatcct
      301 ttctgctcgg tttaatcgtt agtatcatcc gacattatcg aatccttgtt ttgtcgcaag
      361 tagcgacagc ctacattgaa ttgtcacgta atacgcccct tttgattcaa ctcttctttc
      421 tctacttcgg tcttccccga atcgggattg tcctatcttc agaagtctgt gcaacgcttg
      481 ggcttgtctt tttaggagac tcctatatgg cagaatcttt ccgaagtggg ctggaagcca
      541 tcagtcaaac ccagcaggag attggcctcg ctattggtct gacacctcta caggtctttt
      601 actatgtggt tcttccgcaa gcaacagcgg tggcactccc ctcctttagt gccaatgtca
      661 ttttccttat caaggaaacc tctgttttct cagcagtggc tttggccgac ctcatgtacg
      721 tcgccaagga tttgattgat ctctactatg agacagacat tgcgctagct atgttggtag
      781 ttgcttatct gatcatgctg ctacccatct cactggtctt tagctggata gaaaggaggc
      841 tccgccatgc aggattcggg aatccaagta ctctttcaag gaaataatct cctgagaatc
      901 ttacagggat tgggcgttac gattgggata tccatcctgt ctgtcctctt atccatgatg
      961 ttcggaacag tcatgggaat catcatgacc tcccattcta gaatcatacg atttttaaca
     1021 cgattgtatc tggaatttat ccgtatcatg ccccagctgg tgctactctt catcgtttac
     1081 tttggcttgg ctcgaaactt taatatcaat atctcaggtg agacttcagc tattatcgtt
     1141 tttaccctct ggggaacagc tgaaatggga gacttggtac gtggagctat cacttctctc
     1201 cctaaacatc agtttgaaag tggacaggca ctcggcttga ctaatgttca actttactac
     1261 cacatcatca tcccacaagt cttgagaaga ctgctaccgc aggctatcaa tcttgtcact
     1321 cggatgatta aaaccacttc attagttgtt ttgattgggg ttgtggaagt gaccaaagtt
     1381 ggacaacaaa tcatcgatag caatcgcctg accatcccaa ctgcttcatt ttggatttat
     1441 ggaaccattc tagtcttata tttcgcagtt tgctacccta tttccaaact atccactcac
     1501 ttagaaaaac attggagaaa ctaaatgtct gaaactatct tagaaatcaa ggaactaaaa
     1561 aaatccttcg gagacaatcc catcctccaa ggactttctc tagaaatcaa aaaaggggaa
     1621 gttgttgtca tcctagggcc atctggttgt gggaaaagta ccctccttcg ttgcctcaac
     1681 ggcttagaaa gtattcaagg tggagatatt tttctggatg gtcagtctat cgttgaaaat
     1741 aaaaaaaatt ttcacctagt tcgccaaaag attggcatgg tctttcaaag ttatgaactc
     1801 tttccccatc tggatgtctt acaaaacctc atcctaggcc ctatcaaaac tcaaggaagg
     1861 gacaagaaag aagtaacgga agaagctttg caattactag atcgtgtcgg tttgctggat
     1921 aaacaacata gctttgcccg tcaattatct ggtggacaga agcaacgtgt tgcaattgtc
     1981 cgtgccctcc taatgcatcc agaaatcatc ctttttgacg aggtgactgc ttcgctggat
     2041 ccagaaatgg tgcgtgaggt gctggaactt atcaatgatt tggcccaaga aggccgtacc
     2101 atgattttag taacccacga aatgcagttt gcccaagcca ttgctgaccg gattatcttc
     2161 ctcgaccaag ggaaaatcgc tgaagaagga acagctcaag ccttctttac caatccgcaa
     2221 accaaacgag cccaggaatt tttaaacgtc tttgacttta gccaatttgg ctcatatcta
     2281 taaaggagat tcttatgaaa ctattcaaac cactcttaac tgttttagca cttgcctttg
     2341 cccttatctt tatcactgct tgtagctcag gtggaaacgc tggttcatcc tctggaaaaa
     2401 caactgccaa agctcgcact atcgatgaaa tcaaaaaaag gcggtgaact gcgaatcgcc
     2461 gtgtttggag ataaaaaacc gtttggctac gttgacaatg atggttctta ccaaggctac
     2521 gatattgaac tagggaacca actagctaaa gaccttggtg tcaaggttaa atacatttca
     2581 gtcgatgctg ccaaccgtgc ggaatacttg atttcaaaca aggtagatat tactcttgct
     2641 aactttacag taactgacga acgtaagaaa caagttgatt ttgcccttcc atatatgaaa
     2701 gtttctctgg gtgtcgtatc acctaagact ggtctcatta cagacgtcaa acaacttgaa
     2761 ggtaaaacct taattgtcac aaaaggaacg actgctgaga cttattttga aaagaatcat
     2821 ccagaaatca aactccaaaa atacgaccaa tacagtgact cttaccaagc tcttcttgac
     2881 ggacgtggag atgccttttc aactgacaat acggaagttc tagcttgggc gcttgaaaat
     2941 aaaggatttg aagtaggaat tacttccctc ggtgatcccg ataccattgc ggcagcagtt
     3001 caaaaaggca accaagaatt gctagacttc atcaataaag atattgaaaa attaggcaag
     3061 gaaaacttct tccacaaggc ctatgaaaaa acacttcacc caacctacgg tgacgctgct
     3121 aaagcagatg acctggttgt tgaaggtggc cattaactct taaaagggac tggattttaa
     3181 gctccaatcc ctttttaaga ttttacctat aacatcctga gtctatctaa gatgttcaat
     3241 ctgaacacag tgtacatact ttatcttcta ttgcatatac tttatcacat aagatacgaa
     3301 tatcctcttc actatgacta gcaatcaaaa ttgttgtccc tttttcacta gagagctttc
     3361 taaacaatgt tctcatattt tctacacttg atttatccaa ggcattcata ggttcatcta
     3421 gtaaaagaat agagggattc tccataattg cttgagcaat ccctagcttt ttcctcatac
     3481 ctagcgaata agttttaact ttctggtctt tttgctcata tagaccaact attttcagtg
     3541 tatcactgat ttcctgatta ccaactactc ctcgtatgct tgccaaatat tgtaaattct
     3601 taaagccact ataataattt ataaaaccag gttcttcaat caaagctccc aaattagctg
     3661 gaatttttct ctcaggaaca atattttccc cattgattaa cacttctcca taagacggac
     3721 tatataaacc agctattaat ttaaacaata cacttttccc tgagccattc gcaccagtaa
     3781 ttcctataat ttccccctgt ttacaactaa aatta
//
LOCUS       DAVMEI010000147         3717 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVMEI010000147 DAVMEI010000000
VERSION     DAVMEI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.147"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(138..3050)
                     /gene="nanA"
                     /locus_tag="VYQ24_001743"
     CDS             complement(138..3050)
                     /gene="nanA"
                     /locus_tag="VYQ24_001743"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1968848.1"
                     /translation="MNRSIQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDASAPRFYNLFSVSS
                     DTKENEYFTMSVLDNTALIEGRGANGEQFYDKYTDAPLKVRPGQWNSVTFTVEQPTAE
                     LPHGRVRLYVNGVLSRTSLKSGNFIKDMPDVNQAQLGATKRGNKTVWGSNLQVRNLTV
                     YDRALSPEEVQTRSQLFERGDLEKKLPEGAKISEKEDVFEGGMNNKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKEIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKERTIREEF"
     gene            complement(3338..3565)
                     /locus_tag="VYQ24_001744"
     CDS             complement(3338..3565)
                     /locus_tag="VYQ24_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968849.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
BASE COUNT          979 a          847 c          628 g         1263 t
ORIGIN      
        1 tactatattt tatagaacag actactctga aagtagtttc agacctctta tgatttcgta
       61 tcagcctgaa tgtcatcaaa aaaagatagc aggcttgaaa acctgctatc tccttctatt
      121 tttacaaaat caaatgttta gaattcttct cttattgttc tctctttttt ccctagcgta
      181 aacagaccaa ggaagaaagc tgttagtcct agtgaagcta ggaggtcact ctccttgttc
      241 cctgtttcag gaagtgcctg ctgagcaagt ggagctttgt aagtataatc ttcttttgta
      301 gtaagagtta caagcgaatc agatccctta tactctgcga tttcatgaac agctggctct
      361 gtaccattaa ctccccctgt aaattctgga acttcatgca ccgctgcttt gctaccattt
      421 actccgccag gaactctggc acctgctaga tttacaggaa gattatgcat actttcgatg
      481 cttcctttag ctataccaat aatttcctgt ccgatatctt ccttatctac tgcaaacaac
      541 aaggttttgc tatcatactg ggttaggaaa gtcgctgttt taccatttgc caattgaaga
      601 gttggagcct tgttgaccaa tacttctgag tcgaactcca agccaataac tcctttgccc
      661 atctctcctt tgcccatctc tctagttcgc ttcactttcg cttcggtagg agaaatcaga
      721 tttttgctca aaaattccca attaaatttt ctaaatgata gggtataggc attttgtcct
      781 ttttcagtat gttcatacaa gatgccatac tccccatttc ctaattcttg gagcgaatta
      841 taggcaaact ctcctttttg aattggattg tgtttgagcc aagtcaactc accattttct
      901 tcgacacgtg ccaagtggac catcccattt tcacgtttcg gtccacctgc attactgagg
      961 atgatgtatt cttttccttc gtgcatcgta tggatagcag acatttgaac atagacatct
     1021 ttaacctgtg gataacgttt gatatccttc tcccaagtca ctcctccgtc tttacttgta
     1081 gcaacctgaa gatctccagt caaaccacgc ataaagagtt taacatctcc attgtttagt
     1141 tgtaccaccg ttgattctgt attttgcgca cgtttattgt tcatcgtaga agagtggatc
     1201 ttttgaccgt ctacctgacg gttatcgttg accgcttctc cagcatgcca agtttttcca
     1261 tgatcatctg aatagatgac acgagaagat tgcgagccat ttaagtgaga tacattatta
     1321 gtcgtataaa ccggtatcaa aatccgtccc ttgtgaggcc cattccgaag tacaattcct
     1381 gttccaggac ctacacccaa gaatttcatc caatcggctt tgaccatcgg agtaatatct
     1441 tgaggagctg accatgtctt cccgtcgtca tcactgtagg acatccatag atagctatcc
     1501 ttggcaattc taaatgggga agttttgttt gttgtgaagt agatattacc tagtaattgg
     1561 tcacccttgt atagatcacc cttgtcgcta taggctggtt taacaggatc tacaacaacg
     1621 cgatagtctg tcgccttgcc atctggtgtg tagacagtac cattttcacg aatggtataa
     1681 gctccctttt ctccttcacg atagaggatt tgataggttt ttccgtcaat ttctttgtag
     1741 gcttcttctt tttgcgaaga cattccaaag attcccttcc cttctgggaa catgtcatag
     1801 atggaaaaga ttcgtttggt ttcaggatct tgaaccaaca ccatatcgat attcactggt
     1861 gaaccgatcg atggatcaga agcttttgga ttgtcacgta agttggtaat ggttactcgg
     1921 tctccccaag ttttaccatt gtcttcacta cgtctgataa ccataccgat atcaccccag
     1981 tcactagaat gaagacggcg ttcatctgca cctgcaatca gagttccttt atctgtcttg
     2041 agaagagctg gaatacgata actcttaatt ccatctttat ttggtttgtt gttcattccc
     2101 ccttcaaaga cgtcttcttt ctctgagatt ttcgcacctt ctggaagttt cttctccaaa
     2161 tcacctcttt caaatagttg gcttctcgtc tgaacttctt ctgggcttaa ggcacgatca
     2221 tagacggtta ggtttcgaac ttgtagattg cttccccaaa ctgtcttatt gccccgtttg
     2281 gttgctccaa gttgagcttg attaacatct ggcatatctt tgataaagtt accagatttg
     2341 agacttgttc gagataagac accgttcaca tagagacgaa ctcgtccatg aggtaactcc
     2401 gctgtcggtt gttcgacagt aaaggtcacg gaattccatt gtcctgggcg aactttcaaa
     2461 ggagcatctg tatacttatc atagaactgc tctccgttag cgccacgccc ttcaataaga
     2521 gctgtattat caaggaccga catagtaaag tactcatttt ccttggtgtc actggataca
     2581 gagaagagat tgtaaaagcg tggagctgat gcatctggct taaactccat gtgaactgtt
     2641 gcgttttcaa gtttctttag tttatctagt tcacttgata aatcaactct ctgaccattt
     2701 gaagcattgg tttcaacatc ttctttttct atcactgttt cgacattttc caaatctcgt
     2761 gcatagtaat ctcttggaat tttttcttct tgcttatctt tcctttcttg ttcttgctta
     2821 ttgccagaaa gttcagtctc tgaagaaggc tggctctttt ctgtatcagt tagggttgag
     2881 ctcccccccg aaagttgagt ttcatttgcc agaggttgct cacttgcccc ttcttgagct
     2941 aaaacaggag acgttccaaa taccactgct cctacaatca tagaaaccgc tcctaccgat
     3001 agtttcctaa tgctataacg acacttacgt tcttgaatac tccgattcat actgatcctc
     3061 tctatatcta tatcccgatt tctgaaataa gacatttact ccagaaaatg cacttagttt
     3121 gtcttttatt aactttcttt tactacagtt catttaaatg acaaaaatga actaaacaaa
     3181 ctatctcaaa ttggtgccaa ttttcccctc tcctttctcc aatcagactc ctgccaatct
     3241 agtttatcta ccagggcacc gctgcaatta aactaaaata tctctttata agcaaatgat
     3301 ggacatcagc ttatgctatt tataccatac ttcctgctca atccttatcg aacacttttt
     3361 caaatagctt ttcataagca taaatacttg tagaaataat gggaaatata atcaagctca
     3421 caatcagaat cttatccagc ttaaagggga caatcccaaa taagtgaaga aataaatcaa
     3481 tcagaaaact gtcgaaaaaa actgtcaaaa gtagttcaac ttgtcttttt ctctgctttt
     3541 ttgtcttgaa tgccttaaat atcatattat tccccttttc taagcttcaa tcccttctct
     3601 ctatctgtta tttcgcatag ctactactat ttttacagca atctatcttt aaatatacat
     3661 aaagatagat tctatcactt tttataagcg ttagtaaatt tattatacct cattttt
//
LOCUS       DAVMEI010000148         3643 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVMEI010000148 DAVMEI010000000
VERSION     DAVMEI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.148"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            183..1412
                     /gene="arcA"
                     /locus_tag="VYQ24_001745"
     CDS             183..1412
                     /gene="arcA"
                     /locus_tag="VYQ24_001745"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1968850.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1470..2486
                     /gene="argF"
                     /locus_tag="VYQ24_001746"
     CDS             1470..2486
                     /gene="argF"
                     /locus_tag="VYQ24_001746"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1968851.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2651..3598
                     /gene="arcC"
                     /locus_tag="VYQ24_001747"
     CDS             2651..3598
                     /gene="arcC"
                     /locus_tag="VYQ24_001747"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1968852.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         1148 a          710 c          760 g         1025 t
ORIGIN      
        1 atttgtatct atgtattcta tcctatgtta tgtataataa cataaaattg tgctaaacat
       61 cataatttat attattttcc gagaaattat agccattgac attacctagg aattgcgtta
      121 taatatacat gtaagcggta cccaaaatgg tgccaagtca aaatttttaa ggaggaaaat
      181 acatgtcttc tcatccaatt caggtcttct cagaaattgg gaaactgaaa aaagttatgt
      241 tgcaccgtcc aggcaaggag ttagaaaact tgttgccgga ctatcttgaa aggcttcttt
      301 ttgatgatat tcctttcttg gaagatgctc aaaaagaaca tgatgcattt gcccaagctc
      361 ttcgcgatga aggaattgag gttctctacc tagaacaact cgctgctgaa tcattgacct
      421 ctccagaaat ccgcgatcaa tttatcgagg aatacttaga cgaagccaac atccgtgatc
      481 gtcaaactaa ggttgctatt cgtgaattgc ttcacggcat caaggacaac caagaattgg
      541 ttgaaaaaac aatggctggg attcaaaaag ttgaattgcc agaaattcct gacgaagcta
      601 aagatctaac tgacttagtt gaatcagatt atccatttgc aattgacccg atgccaaacc
      661 tctatttcac tcgcgaccca tttgcaacaa ttggaaacgc cgtatcgctt aaccacatgt
      721 ttgcagacac tcgtaaccgt gaaacactct acggtaagta tatcttcaaa taccacccaa
      781 tctatggcgg aaaagtggat ttggtctaca accgtgaaga agatacgcgt atcgaaggtg
      841 gagacgagct agttctttct aaagacgtcc ttgcagtagg tatctctcaa cgtacagacg
      901 cagcttctat cgaaaaactt ttggtcaaca tcttcaagaa aaatgttggc ttcaagaaag
      961 ttttggcctt tgaatttgct aacaaccgta aattcatgca cttggatact gtcttcacta
     1021 tggtagacta tgacaagttc actattcacc cagaaatcga aggcgacctt cacgtttact
     1081 cagttactta cgaaaacgaa aaacttaaaa tcgttgaaga gaaaggtgac ttagctgaac
     1141 ttcttgctca aaaccttggt gtagaaaaag ttcatttgat tcgttgcggt ggtggcaata
     1201 tcgtagcagc tgcgcgtgaa caatggaacg acggttctaa cactttgacc atcgcacctg
     1261 gtgtggtagt tgtttatgac cgcaataccg tgaccaataa gattttggaa gaatacgggc
     1321 ttcgcttgat taagattcgc ggaagtgaat tggttcgggg ccgtggtgga cctcgttgta
     1381 tgtctatgcc atttgaacgt gaagaagtgt aatcgctgtt cggtattcgt caatagaaaa
     1441 tgtaaaaaat agaaagagga aataataaaa tgacaaattc agtattccaa ggacgcagct
     1501 tcttagcaga aaaagacttt acccgtgcag agttagaata ccttattggt ctttcagctc
     1561 acttgaaaga tttgaaaaaa cgcaatattc aacaccacta ccttgctggc aagaatatcg
     1621 ctctcctatt tgaaaaaaca tctactcgta ctcgtgcagc ctttacaact gcggctatcg
     1681 accttggtgc tcacccagaa tacctcggag caaatgatat tcagttgggt aaaaaagaat
     1741 ctactgaaga tactgctaaa gtattgggac gtatgtttga cgggattgaa ttccgcggat
     1801 tcagccaacg tatggttgaa gaattggcag aattctcagg cgttccagta tggaacggtc
     1861 taactgacga atggcaccca actcaaatgc tcgctgacta cttgactgtt caagaaaact
     1921 tcggtcgctt ggaaggcttg acattggtat actgtggtga tggacgtaac aacgttgcca
     1981 acagcttgct cgtaacaggt gctatccttg gtgtcaatgt tcacatcttc tcaccaaaag
     2041 aactcttccc agaaaaagaa atcgttgaat tggcagaagg atttgctaaa gaaagtggcg
     2101 cacatgttct catcactgaa gatgctgatg aagcagttaa agatgcagac gttctttaca
     2161 cagacgtttg ggtatcaatg ggtgaagaag acaaattcgc agaacgtgta gctcttctta
     2221 aaccttacca agtcaatatg gacttagtta aaaaagcagg caatgaaaac ttgatcttcc
     2281 tacactgctt gccagcattc cacgatactc acactgttta tggtaaagac gttgctgaaa
     2341 aatttggtgt agaagaaatg gaagtaacag acgaagtctt ccgcagcaag tacgctcgcc
     2401 acttcgatca agcagaaaac cgtatgcaca ctatcaaagc tgttatggct gctacacttg
     2461 gtaaccttta tattcctaaa gtataatttt agataataaa ccgtctacca acagctatga
     2521 gggctgcgac taatagcttt agtccggtcc tcttttatgt aatggtaatc tattatttct
     2581 tataaaatat gtgaaaaatc attaaattga aatctaaacg cattctattg agtgtgataa
     2641 aggagaattt atggcaaatc gtaaaattgt agtagctttg ggaggaaatg cgattctttc
     2701 ttctgaccca tcagcaaagg ctcaacaaga agctttagtt gaaacagcta agcatcttgt
     2761 aaaattgatt aaaaatggag atgatctgat tatcactcac ggtaatggac ctcaagttgg
     2821 gaatctcttg ctccaacatt tggcatcaga ctctgaaaag aaccctgcct tcccactcga
     2881 ctcacttgtc gctatgacag aaggtagcat cggtttctgg ttgaaaaatg ctttgcaaaa
     2941 tgctctcttg gatgaaggca tcgaaaaaaa tgttgcctct gttgtaacgc aagttgtcgt
     3001 agataaaaat gatccagctt ttgttaactt gagtaaacca atcggtcctt tctattcaga
     3061 agaagaagca aaagcagaag ccgaaaagag cggagcgact ttcaaggaag atgctggccg
     3121 tggctggcgt aaggtcgttg cctcaccaaa acctgttgac atcaaagaaa ttgaaaccat
     3181 ccgtactctt ttaaataatg gtcaagtcgt cgtagctgca ggtggtggcg gtattcccgt
     3241 tgtcaaagaa aacaatggac atttgactgg tgtcgaagcg gttattgata aagacttcgc
     3301 ttcccaacgt ttggcagaat tggttgatgc agacctcttc atcgttttga caggtgtaga
     3361 ttatgtattt gttaactaca acaagccaaa ccaggaaaaa ttggaacatg tgaatgttgc
     3421 ccagctggaa gaatatatca aacaagatca gtttgcacca ggtagcatgc ttccaaaagt
     3481 agaagcagct atcgcttttg tcaatggtcg tccagaagga aaagcagtta ttacttccct
     3541 tgaaaatcta ggcgccttga ttgaatctga aagcggaaca attattgaaa aaggataagt
     3601 tgttttacta ataagatgta ttctatttct agtatcttta tat
//
LOCUS       DAVMEI010000149         3600 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVMEI010000149 DAVMEI010000000
VERSION     DAVMEI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.149"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(122..1258)
                     /gene="lctO"
                     /locus_tag="VYQ24_001748"
     CDS             complement(122..1258)
                     /gene="lctO"
                     /locus_tag="VYQ24_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1968853.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDKRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGTQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1884..3374)
                     /gene="lysS"
                     /locus_tag="VYQ24_001749"
     CDS             complement(1884..3374)
                     /gene="lysS"
                     /locus_tag="VYQ24_001749"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1968854.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYTNLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3502..>3600)
                     /locus_tag="VYQ24_001750"
     CDS             complement(3502..>3600)
                     /locus_tag="VYQ24_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1968855.1"
                     /translation="TYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
BASE COUNT         1025 a          774 c          708 g         1093 t
ORIGIN      
        1 atttaactgt cctgattgat ttgtcctgtt tttattccat tttactatgg ttttcgatag
       61 cgatttattc ttccaataca cgaagaaaaa cctccacatt cagtggaggc aatctctttt
      121 atcaatacaa ttttaagtca cgagggtcaa ctgggaaagt tgggttgtat gggttgtgac
      181 ggagtttgaa gtgtttaaca tcttcaatag tctgagttcc agacaactgc ataactgtct
      241 tcaattccgc attcaagtgt tcaaagactt gacgcacacc aacactaccg ccgagagcca
      301 agccatagat aaccgggcga ccaatagcta ccaagtctgc tcctgaagcc aaggctttaa
      361 agacatgttg accacgacga acaccagagt caaagacaat tggcacacgt ttatcaactg
      421 cttctgccac ttcttgaagc gagtcaaagg cagctggtcc accgtcgatt tgacgaccac
      481 cgtggttggt tacccagata ccagaagctc ctgcagcaag cgaacgttca acgtcctcac
      541 ggcattgtgg tcccttgaca tacacaggaa gtcctgagta ttcagcgata aattctacat
      601 cacgtggaga caagcgttgt ttagctgatt tgtaaacaaa gtccattgat ttaccagcac
      661 cttctggcag gtattcttca acaatcggca tgccaactgg gaagacaaaa ccattacgct
      721 tatccacttc acgattcccc cctacagtag catctgccgt caagacaatc gctttataac
      781 cttcagcctt cacacgatcc atgatgtggc ggttgatacc gtcatcctta ctaaagtaaa
      841 attggaacca atggggtgtc ccttgaaggg cttcagaaat ctctggaagg tcaacagtag
      901 agtaagaact ggttgtataa agagaaccaa actcatgcac accacgcgca gtcgccactt
      961 ccccctgttc attcgctaat ttatgggccg caacaggcgc cataatgatt ggtgaagata
     1021 gtttttcacc tgcaaattca atctctgtac ttggattttc aacatcgcaa agtgtatgag
     1081 gaacgatgag tttgtggtta aaggcgcgga tattctctct taaagtgaaa gtatcttccg
     1141 ccccactagc gatatagcca aatgctgctt taggaataac ttgttgcgcc attggctcca
     1201 aatcataggt attgatgaag tctacatgac cttctgcatt gcttgttttg tatgacataa
     1261 aatgccctcc ttgattaagt aagcgtttgc tttttactac acaactataa taactctttt
     1321 ttcaatcatt ttcaagagtt ttgcttaaaa accatcttat tttttcatac aatatactag
     1381 ccctactttc cttaaactct aaaccttagt ctatattaag tgaatcgttg agattttttg
     1441 ctgccaggca tttgaactac ttttttattt gcggattcta tctactaata ctataaaaaa
     1501 aacaagtgaa taagacacca gaacatcctc taataggacc tatggactta tatcagtccc
     1561 atatatactg tttaagcaag atactatcct agttgacgct aacttcctat cccacttatt
     1621 aattttttat ttatagtgaa tctgaacaaa tcaattgggg aatccaagtc aatttctagt
     1681 actttttgaa cagtttcagc gtactatcgt aaaatgaaat aaaacatgca caaattgctt
     1741 agggaattta aaccaatttc taacaatgtc ttagaaatca aagtgtacta tttcaacttc
     1801 aatatactac acttcatttt ctccttaaat tcagtctctt tttgaatcaa aaaatcccct
     1861 ggtaagagcc agaggataag aatttatttc attgttggga agagcaatac atcacggata
     1921 gttgttgtat cagtgaggag catgcagaga cggtcgatac cgattcccaa accacctgtt
     1981 ggtggcatac cgtattcaag agcttcaatg tagtcatagt cgattcctgt cgcttcatca
     2041 tcaccaagtt ctttggcttt agcttgggct tcaaaacggc taagttggtc gattgggtcg
     2101 ttcaactcag taaaggcatt accgtactcc ttagtcatga taaagagctc gaaacggtca
     2161 gtaaagcgtt ggtcttcagg atttttctta gcgagtggag atacagctac tggatgtcca
     2221 tagacaaagg ttggttggat taaagtttct tcaacaaact cttcaaagaa ggcattgatg
     2281 atgtgaccaa cctcagtgta gtgtttctca actggaactt tcttctcagc agcgatagct
     2341 ttagcttctt ccaaagtcat gtcttgccag aaatcgacac cagtaatttc tctgatagca
     2401 tccaccatat gaacacgctt aaatggttcg ttaatcttga tttcagtacc ttggtagttg
     2461 actgggccat cacctttgac tgatttagca gcgtgttgga taatgccttc agtcaagtcc
     2521 atgatgtctt ggaagtctgc ataagcttgg taaacttcga tagaagtgaa ctcagggtta
     2581 tgagtagcgt ccattccttc gttacggaag atacggccaa tttcatagac acgttccata
     2641 ccacccacga taaggcgttt taagtgaagc tcagtcgcga tacgaagcac catgtcaatg
     2701 ttttgggcat tgtggtgggt gataaatgga cgggcagcag caccaccggc ttcattatga
     2761 agaacaggtg tttccacttc aaggaatcct ttttggtcaa ggtaacgacg gatttcagag
     2821 atgatttttg aacgagtgac aaagcgttca aagctttcac gattagaaat caagtcaagg
     2881 taacgtttac ggtaaattgt ttcaacgtct gtcaaaccat ggaatttctc aggaagagga
     2941 cgaagagcct tagacaagtg tgtgatgtgg gttgccttga tagagagttc tcccatatcc
     3001 gtacgcatca cttcaccttc gacaccaagg aagtcaccaa ggtctgcttt tttgaagatt
     3061 tcgtagtttt cttcaccgac agcatcctta cgaacgtaga tctgaatctg gccttcgcgg
     3121 tcttgaaggt gggcaaaacc aacttttcct ttaccacgtt tggttaccaa gcgtcctgcg
     3181 atagtagctg tttcgttttt atcgtgtaat tgttctttat cgaggttggt atatttatct
     3241 tttaattctt gtgaatttgc agtacgttca aaacgttttc cgaaaggatc gattccttgt
     3301 tcgcggagcg cagccatttt ttcacggcga acgatctgct ggtcatttag ttcttccata
     3361 tgttctgtag acatgggatc ctcctagttt tactcaaaat tgaatacgat gagtcatttt
     3421 ttgcgatact cccattttat cataaataag caagaaattc acggataatc tttaaaaact
     3481 aaaaagaacc tgacgctaaa atcactggaa agaagtgaaa gtatctcccg ccccactagc
     3541 gatatagcca aatgctgctt taggaataac ttgttgcgcc attggctcca aatcataggt
//
LOCUS       DAVMEI010000150         3558 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVMEI010000150 DAVMEI010000000
VERSION     DAVMEI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.150"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(59..358)
                     /locus_tag="VYQ24_001751"
     CDS             complement(59..358)
                     /locus_tag="VYQ24_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1968856.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            760..1464
                     /locus_tag="VYQ24_001752"
     CDS             760..1464
                     /locus_tag="VYQ24_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1968857.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    1523..1610
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            1646..1960
                     /gene="rplU"
                     /locus_tag="VYQ24_001753"
     CDS             1646..1960
                     /gene="rplU"
                     /locus_tag="VYQ24_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1968858.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1976..2320
                     /locus_tag="VYQ24_001754"
     CDS             1976..2320
                     /locus_tag="VYQ24_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1968859.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            2337..2630
                     /gene="rpmA"
                     /locus_tag="VYQ24_001755"
     CDS             2337..2630
                     /gene="rpmA"
                     /locus_tag="VYQ24_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1968860.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2941..3146)
                     /locus_tag="VYQ24_001756"
                     /pseudo
     CDS             complement(2941..3146)
                     /locus_tag="VYQ24_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1070 a          615 c          713 g         1160 t
ORIGIN      
        1 catttttgca cctttcttag cctttgttat cgacaagcat tgttctagct ccttgttttt
       61 agaaaggtaa tttcccagcg aaagcaccca gttttttctt ggtagtttca tcgatttgtt
      121 caagagcaga gttgatggct tgaacggtca tatcagaaag agtctcaagg tcctctgggt
      181 caacgacagc tggattgaaa tcaatgctga caactttctt atcgccagtt aaggtcgctt
      241 ggacaagatc ttgagcagat ttgccaacaa attgcatagc agcaagttca gcttggcttt
      301 gttccatttg tttttgaagt ttttgtgctt gacgcatcat gttttgcatg ttcatcataa
      361 gatttctaaa gttcctttca tctcaagggg ataatccttg ttgttaacaa gtgcctgatt
      421 ttctctccta attgttatta gtagcatagc catcaagccc aagtattgat tttattatac
      481 cattttttta agacatagtc ttttattggt gagagttatt tatgtcaagg agtgattaga
      541 aataaattaa aataatgatt tttgctaaga gggctctatt ctagtttcat ggaaatgatt
      601 ttactaaagg ttcgtatgct ttgtttgcaa acatgttaca tctatctatt tttggataaa
      661 tagttgtcta ttattatttt tatgctaata gtaaaatcta aatggtagcc tacgattttt
      721 tagaaagaaa atggtacaat atttctaaga gaaaatacaa tgggaggtaa aatgaggtta
      781 ttacctataa gaaaaatatc acgtcagtct aaaaggttag cacttttttt gacgttttgt
      841 gctggatatg tggatgctta cacttttatt gttcgcggga atacccttgt agctggacaa
      901 actggaaatg ttgtctttct ttcagtagaa ttaattaaaa ataatgtttc ggatgttagg
      961 gacaaggttc tcaccttgct agcgtttatg atgggagtct ttttattaac gatttataag
     1021 gaaaaattga gaattgtgaa aaaacctatt ctgtccttga ttcccttggc aatcttatca
     1081 atcattattg cttttgtgcc gcaaactgtg gataatatct atctagtgcc gcccttggcc
     1141 ttctgtatgg gactggtgac aactgctttt ggagaagtgt cgggtattgc ctataataac
     1201 gcttttatga cagggaatat caaacggacc atgctggctt ttggagatta tttccgaacc
     1261 aagcacactc cttttttgcg tgaaggattc atatttgtta gcctgcttag tagttttgtc
     1321 cttggcgttg tcttttcagc ctatttgacg attttctatc atgaaaagac cattcttggt
     1381 gttcctatta tgatgagcgt tttttacctc agcatgcttt ttgcctcttg gcagaaaaaa
     1441 gtaaaagaaa aagcttcatt ttaggtgtta ttacttgtaa gaaaatagga gatatgctat
     1501 acttgaagag ttgactatgc acgatcctgt gcaaccgcac gaacatcgtt gcttgattat
     1561 tcaagtttaa gtggttcgtc ccacgatgct aggcgagtct tcacaaaaaa tcggggaaac
     1621 ccataaaaat cataggaggt gcataatgag cacatacgca attatcaaaa ctggcggaaa
     1681 acaagttaaa gttgaagttg gtcaagcagt ttacgttgaa aaattgaacg ttgaagctgg
     1741 tcaagaagtt acttttaacg aagttgttct tgttggtggt gaaaacactg ttgtcggaac
     1801 tccacttgtt gctggagcta ctgtagttgg aactgttgaa aaacaaggaa aacaaaagaa
     1861 agtggttact tacaagtaca aacctaaaaa aggtagccac cgtaaacaag gtcaccgtca
     1921 accatataca aaagttgtca tcaacgcaat caacgcttaa ttttaaggag aacacatgat
     1981 acaggcagtc tttgagagag ccgaagatgg cgagctgagg agtgcggaaa ttactggaca
     2041 cgccgagagt ggcgaatacg gcttagatgt cgtgtgtgca tcggtttcta cgcttgccat
     2101 taactttatc aattctattg agaaatttgc aggctatgaa ccaatcctag aattaaacga
     2161 agatgaaggt ggctatctga tggttgaaat accaaaagat cttccttcac accagagaga
     2221 aatgacccag ttattctttg aatcattttt cttaggtatg gcaaacttat cggagaacta
     2281 ttctgagttc gtccaaacca gagttatcac agaaaactaa cacggaggaa aacattatgt
     2341 taaaaatgac tcttaacaac ttgcaacttt tcgcccacaa aaaaggtgga ggttctacat
     2401 caaacggacg tgattcacaa gcaaaacgtc ttggagctaa agcagctgac ggacaaactg
     2461 taacaggtgg atcaatcctt taccgtcaac gtggtacaca catctatcca ggtgtaaacg
     2521 ttggtcgtgg tggagacgat actttgttcg ctaaagttga aggcgtagta cgctttgaac
     2581 gtaaaggacg cgataaaaaa caagtttctg tttacccaat cgcaaaataa aaaggttcat
     2641 tgaacctttt atcccgaacc ttgaaatgta aaggtgagga agctagaaac agcttaaaat
     2701 aaggctttcc gagttttcgg agagcctgtt tttttatttt gatacccata ctttttaata
     2761 tgtatagtgg attgatactc ttcgaaaatc aaattcaaac cacgtcaatt tcaccttgcc
     2821 gtactcaagt acagcctgcg gctaacttcc tagtttgcac tttaattttc attgagtatg
     2881 aatctggaat agaacactgt taaaaattga tatgagtttc ctactcgatt tcttcagatt
     2941 ttatttcaat tgactataag aactgctatc tggtttcaaa tattggcttg tcgtcccttc
     3001 acttgtctga atggtttgag ccgttccttc tgataaggga aaagatattc tttagctgaa
     3061 acggtctgag aatccaagct aacagatgga gagaaaaata gcccagtctg aagcacacta
     3121 gatgggagtt gttggaaagc agtcatctag tttttctttg ctgttttggc ttttttcttt
     3181 gtcatcaggg aaagtgactt gcttcaaatc agggagttta ggaaattctt caaacatctt
     3241 gcccaagttg tctcattttg taaattttac agccataggt gtacctcgat tctaaattct
     3301 acctttttgg caccaattag taatttaaag ttctcaggag ttagttgcat tgctttttca
     3361 tacataacat agtatgacac tttatggtta ccgaacaagt ctaatcactc cttgcaaaat
     3421 tttgaagatg tcctacaagt ttttgagaag aatattgcaa agttcatagt taagcaacga
     3481 tagaataaag tgctttttga aaataggtac acttcatgtg aaaaaaacaa catttcagat
     3541 tttctgttcc agaaatag
//
LOCUS       DAVMEI010000151         3515 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVMEI010000151 DAVMEI010000000
VERSION     DAVMEI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.151"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            26..1435
                     /locus_tag="VYQ24_001757"
     CDS             26..1435
                     /locus_tag="VYQ24_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1968861.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKSIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1453..2748
                     /locus_tag="VYQ24_001758"
     CDS             1453..2748
                     /locus_tag="VYQ24_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1968862.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVNRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2753..3061
                     /locus_tag="VYQ24_001759"
     CDS             2753..3061
                     /locus_tag="VYQ24_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1968863.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3058..3366
                     /locus_tag="VYQ24_001760"
     CDS             3058..3366
                     /locus_tag="VYQ24_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1968864.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1183 a          600 c          684 g         1048 t
ORIGIN      
        1 tagaataata gaaaggaggt tgagcatgaa aaaaaagtta gtatttccta atctgttttg
       61 gtggggagca gcttctagcg gacctcagac agaaggtcaa tatggaaaag tacatgaaaa
      121 tgtgatggac tactggttca aaacgcatcc agaagatttt ttcgataatg tcggacctct
      181 tgtagccagt aacttttttc atacttacac cgaagatttc cacttgatga aggaaattgg
      241 agttaattct ttccgcactt ccatccaatg gagtcgactc atcaagaatt tagagacagg
      301 tgagcctgat ccaaaaggta ttgctttcta caatgccatc attgaagaag ctaaaaagaa
      361 ccagatggat cttgtgatga atttacatca ttttgattta ccagtggaac ttcttcaaaa
      421 atacggtggt tgggaaagca aacatgtagt ggagttattc gtgaagtttg ccaagactgc
      481 tttcacatgc tttggagata aggttcatta ctggacaact ttcaatgagc caatggtcat
      541 tccagaagca ggatacttat atgctttcca ttatccaaat ctaaaaggaa agggaaaaga
      601 ggccgtacaa gtcatctata atctaaacct tgctagtgca aaagtgattc aactatatcg
      661 ctcattagaa cttgatggaa agattgggat tattttaaac ttgacacctg cttatccaag
      721 aagtaattct ccagaagact tagaagcaag tcgatttaca gatgacttct ttaacaaagt
      781 cttcttgaat ccagctgtta aaggaacttt cccagaaaga ttggtaaaac agctagagag
      841 agatggcgtg ttatggagtc ataccgaaaa agagcttcaa ctgatgaaat caaatacggt
      901 tgattttctt ggagtaaact actaccatcc aaaacgtgtt caagcacaag caaatcctga
      961 ggaatatcag acgccctgga tgccagacca atacttcaaa gagtatgaat ggccggagcg
     1021 tcgcatgaat ccatatcgtg gttgggaaat ttttccgaaa tccatttatg atattgctat
     1081 gattgtgaag gaagaatatg gtaatatccc atggtttatc agtgaaaacg gaatgggtgt
     1141 tgaaaacgaa gcacggttta tcgatgaaaa tggagttatc gatgacgtgt atcgtattga
     1201 attttatgaa gaacatttaa gatggctaca taaagccatt gaagagggaa gtcactgttt
     1261 tggataccac gcttggaccg catttgattg ctggtcttgg aataatgcat ataagaatcg
     1321 ttacggattt atctccgttg atttagaaac gcaaaagaga accatcaaga gctcaggaag
     1381 atggtatcgc aaagtaagtg acaataacgg ttttgaagta gaaattgagg agtaaagaga
     1441 ggagatattc atatgaaaaa ctcaaaattt atagaccaat ttgccacctt tgctggtaaa
     1501 ctagggaacc aaattcattt aaaaacccta agagatgcat tcgtaacagt aatgccatta
     1561 tatattttgg caggtttaat cgttcttttg aacaacacgg tatttaagtg gattttccaa
     1621 ggggatacat taacaagatt ccaatattgg ggaataacaa ttgcaaacgg tactttaagt
     1681 atttcaggta tgattattgc tgtaatggtt ggttatttct tagctaaaaa cagagatttc
     1741 gaaaacccgt tagcagcatc aatgctatca ttagtttctt taattgtgat gatgccaaat
     1801 acagtttctg tagttcctga cggagcaaaa gatgctgtaa acatttcagg tgttctttca
     1861 ttcaacaaca caggtacagg cgcaatgttc gccggggtta tcgtagcgat tattgcaaca
     1921 gaattattca ttgaattatc aaatgttaaa gctttacaaa tgaaccttgg tgaaaatatt
     1981 ccaccagctg ttaatagatc atttagcgta ttacttccag tcatgaccgt catctcctta
     2041 tttggggttg tttcagcatt attattcaat ataactggaa tgaacttaat ctcaatcatt
     2101 acaatcttta ttcaagaacc aattcgtcat attggtacaa gcttaatcgg ggtcattatt
     2161 atttactctt taggaaatat gttatggcta tttggtattc accaagcagt tatttacagt
     2221 gccatcctag aaccattact attaattaac attactgaaa acatcactgc agcaaataat
     2281 ggacaagcca ttccacacat catcaaccta tcacaaatac aaacattcgc tttaatgggt
     2341 ggtagtggat ctacattatg tttattaata gcaacattct tagtgagtcg caatgctgtc
     2401 tctaaaaacg tggctaaatt atcttttgga cctggtatct tcaatatcaa tgaaccagta
     2461 ttattcggtt acccaatcgt ttataacatt tcattagcta ttccatttat cacagttcca
     2521 gtccttggta ttttaatcag ctacttagca acagttacag gattcatgag tcctgcattt
     2581 atacaagttc cttggactac accagtattc ttaaatgcat ggttagcaac agcaggggac
     2641 gtgagagcag ttctagttca attcatcatc tttgcacttg gagttcttct atacattcca
     2701 tttatcaaag ttaatgacaa agttgttgaa caagaaatgg aaggttaaga ttatgaaaca
     2761 aattttatta gtatgtaatg cgggaatgtc gacaagtatg cttgttaaaa aaatgcaaca
     2821 aagtgcgact gaacgtggga ttgaaatctc tatccaagca aaatcaatga cagaagctaa
     2881 aaagaatatc catgaagcag atgtcattct aattgggcca caaattcgtt atgagttact
     2941 tgctgtgaaa gaaattgcag gaaatatccc tgtagatacc attgatatgc gggattacgg
     3001 aatgatgaat ggagcaaaag ttctggaaca agcacttgca tggataggag aaataagatg
     3061 acagaagaaa tggaatatat ctgttttcag ctcattgcca atagtggagc agccaaatca
     3121 tcctttattg aagccatcca actagctaag gcaggaaatt taaaagaagc gaaaattaaa
     3181 gttgaagaag cggaagactc cttagtagaa gcgcacaaga ttcactctaa tctcatacaa
     3241 aaagaagcta ctggagaaaa aataggattc tccttgctat ttatgcatgc agaagaccaa
     3301 atggcttcaa cagagattat tcagttgcta tctaaagaat tcatcgatct ctatcaaaat
     3361 aaataggaac actgtcattc ccatctgtct ttattaatga cgactacatt gtaggaacct
     3421 ccaaaagttg gacaaaatga ttgaaaggat tggatcctgc attacacaag atccaatcct
     3481 tttcttttac tctagttttt ataatatgtt gaatc
//
LOCUS       DAVMEI010000152         3496 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVMEI010000152 DAVMEI010000000
VERSION     DAVMEI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.152"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1225
                     /locus_tag="VYQ24_001761"
     CDS             <1..1225
                     /locus_tag="VYQ24_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1968865.1"
                     /translation="KNNVTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIF
                     GTNVAESVGKILAPLFSATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANI
                     EANVQLVQAGMHADKILNPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASV
                     VPTFFGVNEPILFGAPIVLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTT
                     PAPIGIVMGTALAPLSFVLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEK
                     VAANFNTAKADAILEKAGVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNV
                     PVKAAAGGYGAHREMLPEFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLT
                     RDGKGALAFVQEQFD"
     gene            1440..2846
                     /gene="lacG"
                     /locus_tag="VYQ24_001762"
     CDS             1440..2846
                     /gene="lacG"
                     /locus_tag="VYQ24_001762"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1968866.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDAPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            2936..3148
                     /locus_tag="VYQ24_001763"
     CDS             2936..3148
                     /locus_tag="VYQ24_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1968867.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         1092 a          596 c          693 g         1115 t
ORIGIN      
        1 taaaaataat gtaaccatta gacttcctga agaggttcca ccaaatattg cacaagtttt
       61 taaagatgtt attccgtttg ccttatctgt tctttcaata tatggattgg atttaattgt
      121 tagaaatatt tttggtacaa atgtagcaga atctgttggt aaaatattag cccctctatt
      181 ttcagctact gatggttata tcggtttagc tattgtattt ggtgcttatg ctttcttttg
      241 gtttgttgga attcatgggc catctgttgt agaaccttta attgtagcta tcagttatgc
      301 aaatatagaa gctaatgttc agcttgtgca agctggtatg cacgcagata aaattttaaa
      361 tccagttact caaacttttg ttgtaacaat gggtggtact ggtgcaacac tagtagttcc
      421 atttatgttt atgtggttat gtaaatcaaa aagaaataga attgtcggac gtgcgtctgt
      481 agttcctact ttctttggcg taaatgaacc aatcctattt ggtgctccaa ttgttttaaa
      541 tcctatcttc tttattccgt ttgttactgc tcctattatc aatgtttgga ttatgaaatt
      601 ttttgtagat gttctacaaa tgaatagttt cagtattatt ttaccttgga caacacctgc
      661 tccaattggt attgttatgg gaacggcgct ggctccattg tcattcgtct tggctataac
      721 tttaatcatt atcgacactt taatctatta tccatttgtt aaagtttatg accatcaaat
      781 tttagaagaa gaacggaaag gaaactcttc atctgaattg aaagaaaaag ttgctgcaaa
      841 cttcaacact gcaaaagcgg atgctattct tgaaaaagca ggtgtcgatg cagcacaaaa
      901 taccatcact gaagaaacaa atgtcctcgt tctctgtgct ggtggaggta caagtggact
      961 ccttgcaaat gctttgaata aggcagcagc agaatacaat gtccctgtga aagcagcagc
     1021 aggcggctat ggtgctcacc gtgaaatgtt accagagttt gatttggtta tccttgctcc
     1081 tcaagttgct tcaaactttg aagatatgaa ggcagaaaca gataagctcg gtattaaact
     1141 agcgaaaaca gaaggcgctc aatacatcaa attaactcgt gatggaaaag gtgccctagc
     1201 attcgtacaa gaacaattcg attaaggcta ggaactgtga aacagtctcc tatcgttacg
     1261 gaaatcgcta tggcgaattt cctaatcgcc ttattaattc gtcggtaaaa agatatcgtt
     1321 tttacctcct catgtcacaa ttcggtgact tggtacaaga agtgagatgg agaaggatgg
     1381 ctcactgact cctctcctct cacttttact ttatttaaat caagaaatag gtgaaaaaaa
     1441 tgacaaaaac acttccaaaa gactttattt ttggtggcgc aacagctgcc tatcaagcag
     1501 aaggtgctac acatactgat ggtaaaggac cagtagcgtg ggataaatat cttgaagata
     1561 actactggta cactgcagaa ccagccagtg atttttacaa tcgatatcca gttgatctca
     1621 agctagcaga agagtatggt gtcaatggca ttcgaatttc tattgcctgg tcacgtattt
     1681 tcccgactgg ttatggtcaa gtaaatgcta aaggtgttga gttttatcat aatttatttg
     1741 cagagtgtca caaacgtcat gttgagccct ttgtaactct tcatcacttt gacgcgccag
     1801 aagctctcca ctcaaatgga gacttcttaa accgtgaaaa tatcgaacat tttgtagact
     1861 acgctgcctt ctgttttgaa gaatttccag aagtaaacta ttggacaacc ttcaatgaaa
     1921 ttggaccaat cggtgatggt caatatttgg ttgggaaatt ccctccaggt atccagtacg
     1981 accttgccaa agtctttcaa tcacaccaca atatgatggt gtctcatgca tgcgcggtaa
     2041 aattgtacaa agagaaaggc tataaagggg aaattggtgt tgttcacgcc ctgcctacta
     2101 aatatcctct agatcctgaa aatccagcag atgttcgtgc agcagagttg gaagatatca
     2161 tccacaataa attcatctta gacgcaactt atctaggtcg ctattcagct gaaaccatgg
     2221 aaggtgtcaa ccatatctta tcagtcaatg gtggtagttt ggatcttcgt gaagaagatt
     2281 ttacagcatt agaagctgca aaagacttga atgatttcct aggaatcaac tactatatga
     2341 gtgactggat ggaagccttt gatggagaaa ctgaaattat ccataatggt aaaggtgaaa
     2401 aaggaagctc taagtatcaa atcaaaggta ttggtcgtcg tgtagctcct gactatgtat
     2461 cacgcacgga ttgggattgg attatctacc ctcaaggttt gtatgaccaa atcatgcgtg
     2521 tgaagaaaga ttatcctaac tacaagaaga tttacatcac tgaaaatggt ctcggctata
     2581 aagatgagtt cgttgataac actgtttacg atgatggtcg tattgattac gtgaaacaac
     2641 acttggagat cttgtctgat gcgattgcag atggagctaa tgtaaaaggt tacttcattt
     2701 ggtcattaat ggatgtcttc tcatggtcaa acggttatga gaaacgttat ggtctcttct
     2761 acgtagattt tgaaactcaa gaacgttatc ctaagaaatc agctcactgg tacaagaaag
     2821 tagcggaaac tcagattata gactagtaga attagtcatt agatatagaa ttttagtgag
     2881 tcaaaaagat gttcaaagat tttatccaat ctatttatga aaaaaagttt atattataaa
     2941 tttcgaaaaa tgctctcaaa taccgtgttt gacgagtgaa gaattgaaaa gtcttggaaa
     3001 atggtatgtc tcgactggta aagaatggat ttgtcattca gatgatgagc tggaagaatt
     3061 taaaaatcta tttttaaatt ttatcaatcc tgaagaatgg gatactatct cctttgattc
     3121 agattttatg ccgtttcaac aatcgtaacc aatttctcaa aaaagttaaa tcttatattt
     3181 agtactctgt aaaactctta tctaatcacg ttgcttatac tcaatgaaaa tcaaagagca
     3241 actttaaact aggaagcgag tcgcagattt ctcaatgcat agctttgagg aattgggcaa
     3301 aaagtctttg atatagaaaa aagcatagta tcaggtgttg aaacacctga tactatgctt
     3361 tttattgtgg gaagatttac tttttttctt ctgaaattga gttgttaccc aggctctttc
     3421 agtttattaa ggcttgatga ctttaatgtg tttagatagc ttaaaaagga ttgaatcact
     3481 tagtttagaa tctgaa
//
LOCUS       DAVMEI010000153         3495 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVMEI010000153 DAVMEI010000000
VERSION     DAVMEI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.153"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            2..>3495
                     /locus_tag="VYQ24_001764"
     CDS             2..>3495
                     /locus_tag="VYQ24_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1968868.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VLDKETENKEKHEDIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFK"
BASE COUNT         1357 a          523 c          692 g          923 t
ORIGIN      
        1 aatgaaaaaa agtacagtat tgtcattaac cacagctgca gttattttag cagcctatgc
       61 ccctaatgag gtagtcttag cagacacatc tagctctgaa gatgctttaa acatctctga
      121 taaagaaaaa gtagtattag ataaggaaac agaaaataaa gagaaacatg aagatattca
      181 taatgctata gaaacttcaa aggatactga agaaaagaaa acaacagtta ttgaggaaaa
      241 agaagttgtt agtaaaaatc ctgtgataga cactaaaact agcaatgaag aagcaagaat
      301 caaagaagat tccaatcaat cccaaggaga taatgcacac tcgtctgcaa ataaaggcac
      361 agaaaatccg aaaaaagaag atagacttgt ctatattgct gaatttaaag ataaagaatc
      421 tggagaaaaa gcaatcaagc aactatccaa tcttaagaat acaaaagttt tatatactta
      481 tgatagaatt tttaacggta gtgccataga aacaactcca gataacttgg acaaaattaa
      541 acaaatagaa ggtattacat cggttgaaag ggcacaaaaa gtccaaccca tgatgaatca
      601 tgccagaaag gaaattggag ttgaggaagc tattgattac ctaaagtcta tcaatgctcc
      661 gtttgggaaa aattttgatg gtagaggtat ggtcatttca aatatcgata ctggaacaga
      721 ttatagacat aaggctatga gaatcgatga tgatgccaaa gcctcaatga gatttaaaaa
      781 agaagactta aaaggaactg ataaaaatta ttggttgagt gataaaatcc ctcatgcgtt
      841 caattattat aatggtggca aaatcactgt agaaaaatat gatgatggaa gggattattt
      901 tgacccacat gggatgcata ttgcagggat tcttgctgga aatgatactg aacaagacat
      961 caaaaacttt aacggcatag atggaattgc acctaatgca caaattttct cttacaaaat
     1021 gtattctgac gcaggatctg ggtttgcggg tgatgaaaca atgtttcatg ctattgaaga
     1081 ttctatcaaa cacaacgttg atgttgtttc ggtatcatct ggttttacag gaacaggtct
     1141 tgtaggtgag aaatattggc aagctattcg ggcattaaga aaagcaggca ttccaatggt
     1201 tgtcgctacg ggtaactatg cgacttctgc ttcaagttct tcatgggatt tagtagcaaa
     1261 taatcatctg aaaatgaccg acactggaaa tgtaacacga actgcagcac atgaagatgc
     1321 gatagcggtc gcttctgcta aaaatcaaac agttgagttt gataaagtta acataggtgg
     1381 agaaagtttt aaatacagaa atataggggc ctttttcgat aagaataaaa tcacaacaaa
     1441 tgaagatgga acaaaagctc ctagtaaatt aaaatttgta tatataggca aggggcaaga
     1501 ccaagatttg ataggtttgg atcttagggg caaaattgca gtaatggata gaatttatac
     1561 aaaggattta aaaaatgctt ttaaaaaagc tatggataag ggtgcacgcg ccattatggt
     1621 tgtaaatact gtaaattact acaatagaga taattggaca gagcttccag ctatgggata
     1681 tgaagcggat gaaggtacta aaagtcaagt gttttcaatt tcaggagatg atggtgtaaa
     1741 gctatggaac atgattaatc ctgataaaaa aactgaagtc aaaagaaata ataaagaaga
     1801 ttttaaagat aaattggagc aatactatcc aattgatatg gaaagtttta attccaacaa
     1861 accgaatgta ggtgacgaaa aagagattga ctttaagttt gcacctgaca cagacaaaga
     1921 actctataaa gaagatatca tcgttccagc aggatctaca tcttgggggc caagaataga
     1981 tttactttta aaacccgatg tttcagcacc tggtaaaaat attaaatcca cgcttaatgt
     2041 tattaatggc aaatcaactt atggctatat gtcaggaact agtatggcga ctccaatcgt
     2101 ggcagcttct actgttttga ttagaccgaa attaaaggaa atgcttgaaa gacctgtatt
     2161 gaaaaatctt aagggagatg acaaaataga tcttacaagt cttacaaaaa ttgctctaca
     2221 aaatactgcg cgacctatga tggatgcaac ttcttggaaa gaaaaaagtc aatactttgc
     2281 atcacctaga caacagggag caggcctaat taatgtggcc aatgctttga gaaatgaagt
     2341 tgtagcaact ttcaaaaaca cggattctaa aggtttggta aactcatatg gttccatttc
     2401 tcttaaagaa ataaaaggtg ataaaaaata ctttacaatc aagcttcaca atacatcaaa
     2461 cagacctttg acttttaaag tttcagcatc agcgataact acagattctt taactgacag
     2521 attaaaactt gatgaaacat ataaagatga aaaatctcca gatggtaagc aaattgttcc
     2581 agaaattcac ccagaaaaag tcaaaggagc aaatatcaca tttgagcatg atactttcac
     2641 tataggcgca aattctagct ttgatttgaa tgcggttata aatgttggag aggccaaaaa
     2701 caaaaataaa tttgtagaat catttattca ttttgagtca gtggaagaaa tggaagctct
     2761 aaactccaac gggaagaaaa taaacttcca accttctttg tcgatgcctc taatgggatt
     2821 tgctgggaat tggaaccacg aaccaatcct tgataaatgg gcttgggaag aagggtcaag
     2881 atcaaaaaca ctgggaggtt atgatgatga tggtaaaccg aaaattccag gaaccttaaa
     2941 taagggaatt ggtggagaac atggcataga taaatttaat ccagcaggag ttatacaaaa
     3001 tagaaaagat aaaaatacaa catccctgga tcaaaatcca gaattatttg ctttcaataa
     3061 cgaagggatc aacgctccat catcaagtgg ttctaagatt gctaacattt atcctttaga
     3121 ttcaaatgga aatcctcaag atgctcaact tgaaagagga ttaacacctt ctccacttgt
     3181 attaagaagt gcagaagaag gattgatttc aatagtaaat acaaataaag agggagaaaa
     3241 tcaaagagac ttaaaagtca tttcgagaga acactttatt agaggaattt taaattctaa
     3301 aagcaatgat gcaaagggaa tcaaatcatc taaactaaaa gtttggggtg acttgaagtg
     3361 ggatggactc atctataatc ctagaggtag agaagaaaat gcaccagaaa gtaaggataa
     3421 tcaagatcct gctactaaga taagaggtca atttgaaccg attgcggaag gtcaatattt
     3481 ctataaattt aaata
//
LOCUS       DAVMEI010000154         3408 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVMEI010000154 DAVMEI010000000
VERSION     DAVMEI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.154"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            915..1274
                     /locus_tag="VYQ24_001765"
     CDS             915..1274
                     /locus_tag="VYQ24_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968869.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1284..1649
                     /locus_tag="VYQ24_001766"
     CDS             1284..1649
                     /locus_tag="VYQ24_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1968870.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYEQLVFELRRIGNNINQIARAINQSHLISQNQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            1636..>3408
                     /locus_tag="VYQ24_001767"
     CDS             1636..>3408
                     /locus_tag="VYQ24_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1968871.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSHAEMVEMYNVNFSNNDKLYESRNDRQEKHQQTIHAHYLIQSFSPEDNLTPEEINR
                     IGYEIMMELTGGRFKFIVATHTDKDHVHNHILINAIDRNSDKKLIWNYALERNLRMIS
                     DRISKVTGAKIIEKRYSYRDYKKYRESSHKFELKQRLYFLLQQSKSFDDFLEKAKQLH
                     VQIDFSQKHSRFLMTDRTMIKPIRGRQLSKRDLYDEDFFKTYFAKQEIESRLKFLLKS
                     VYSLEELHVKAKELNLTIELKQKNVMFTLEEDGKKISLSHKKISDKKLYDVQFFNRYF
                     EDREVRDIQALENLQEDFQTFREEQHKEKVSAEEIEEAFKKYKEKRDAIHEFEIELTD
                     NQIEKLVDDGVYIQVSFGIKQSGLIFIPNYQLDIFEEDNHKKYKVYIRETSSYFVYNK
                     ENMDNNCFIKGRTLIRQLSNDSQKLPYRRPTLKSLKEKISEINLMIELSNTNKQYQEI
                     KDELVLEIAEIDMQLEETQEKIATLNKMAEVLINLKSEDHETRKLAKYDFDQMNMTES
                     IMLDRLNTDILKLQQELGNEINKYE"
BASE COUNT         1230 a          532 c          690 g          956 t
ORIGIN      
        1 aaatatataa aaaatcatat catcaatcaa ttagagggag gaaaagattc cttcaagcat
       61 aaaatgtcta tatatgagac ggctttatca gagtagagtc agaaaaagta ggttagaaat
      121 ttagcctact tttttctttg tccgacaggc atagtgtgca tctgaggtcc aagtcctctg
      181 tggatatttg ctgcagatga aaccaatagc gactcctaag cctgaatatc gtgaggtagg
      241 ggggatagga aggaattagc gaaatcaagg ttctacaaac agaatcgtga cttgaagcca
      301 tatatagcgg atgaggaact ctaaaatcca aataggtgtc gtaacctata tacgtaaatt
      361 acgagagtaa actaggaaag atgtacggct tattccgtga gcgtttagga cgtagtacaa
      421 cgaatcatgg gagtcagctg aacacatagt attgaagaaa tttctgtaat ggaaatggag
      481 cgaagaagtg aacaattaaa tgaatacctc tctaattaaa tttgtcaatt ctaattcctg
      541 gtatgaaaag acagtgacct gaaaatgtaa acgatgggag ctgattataa atataggacg
      601 gtacatgcag tggtgttaga gattagtccc tacttgattt gtgataactt ccccaaattt
      661 cttctgctat acttttctca acttttaaaa atccaactaa gaattttacc tgggggtttg
      721 ggggcgaagc caccaagtta tcttatcgtt ggctgtcaaa actggaaggt tttggttagc
      781 tggcgatatg attttttggg tattgtaacc acaatgcttg agctcgcaaa gaccgaacaa
      841 ggactttaag aaatttcccg tatgacacac ggggtcgggg tttacccgag aaaggatgaa
      901 agtgaggaaa agagatgtct gaacagtacc gtgacattcg aaaagaggta aatctaaccg
      961 cagatgagct aaaacagatt gaaaaaatga tggaggttga taattatcgt cacttttcac
     1021 cattcgtcag ggataaaatt ttgatgactg atgacaaaca gttagctgcc aaagaatggt
     1081 tttccctttg gcagtctcaa aagtttgaac agatcagtcg agatgtgcat ttggttttga
     1141 ttatcgcaag agagaatcac caagtgactc aagaacacgt gtcaatctta ttgacctgcg
     1201 ttcaagaatt gattgcggaa gtcaatcaag tacagtcact cagtcgtggg tttcgtgaaa
     1261 aatacatgag ataggaggct actatggttt atcgttatcg taccaatctc aaaaaagtat
     1321 ttctaacaga ttcagaatta caccaattga atgaacggat tgctaagagc aattgtcaaa
     1381 atttctcagt ctatgccaga aaagttttgc ttaatcccaa tatgtcattt gtcacgatta
     1441 acactgatac ttatgaacag ttagtgtttg aattgaggag gattggaaat aacatcaatc
     1501 aaattgcgcg tgcgattaat caaagccatc tgatttctca gaaccaatta caagaattga
     1561 gtaaaggagt cggagagtta attaaggaag tggacaaaga atttcaagtg gaggtgaaaa
     1621 gactgaagga gtttcatggt agttactaaa cactttgcga cgcacggaaa aaaatatcgt
     1681 aggcgtttaa ttaagtatat tctcaaccct gataaaacgg acaatttgaa attggtatct
     1741 gattttggca tgagcaatta cttggacttc cctagtcatg cagaaatggt agagatgtac
     1801 aatgtcaact tcagcaataa tgacaagttg tacgaatcca gaaacgaccg acaagaaaaa
     1861 caccaacaaa ctattcatgc tcattacctc attcaatcat tttctcctga agataatctg
     1921 acacctgaag aaattaaccg cattggttat gagatcatga tggaattgac aggaggtcgt
     1981 tttaagttta tcgtagcaac tcacacagac aaagatcatg ttcataatca catcctaatc
     2041 aacgctattg accgcaattc agataaaaag ttgatatgga actatgcctt ggaacgaaac
     2101 ctccgtatga tttcagaccg tatttctaaa gtgactggtg caaaaattat tgagaaacgt
     2161 tattcctatc gagattataa aaaatatagg gaatctagtc ataagtttga attgaaacaa
     2221 cgtctttatt ttttgctaca gcaatctaaa tcgtttgatg attttttaga gaaagcaaag
     2281 cagttgcatg ttcagattga ttttagtcaa aagcatagcc gttttttgat gacagataga
     2341 acgatgataa aaccaattcg aggacgccaa ctcagcaaac gagatttata tgatgaagac
     2401 ttttttaaaa catattttgc caaacaagag attgaaagtc gtttaaaatt tttactaaag
     2461 tctgtttatt cattagaaga attgcatgta aaggctaaag agttgaatct aaccatcgag
     2521 ttaaaacaga aaaatgtgat gtttacccta gaagaagatg gcaaaaagat aagcctgagt
     2581 cataaaaaaa ttagcgataa gaaattgtac gatgttcaat tttttaatcg ttattttgaa
     2641 gatagagaag ttagagatat tcaagcatta gaaaatcttc aggaagattt tcagactttt
     2701 cgagaggagc agcataagga gaaagtctcc gctgaagaaa tcgaggaagc cttcaagaaa
     2761 tacaaggaaa aacgagatgc tatccatgag tttgagattg aacttacgga taatcagata
     2821 gagaagttgg ttgatgatgg tgtctatata caggtttcgt ttggtatcaa acagagtgga
     2881 ctcatcttta ttcctaacta ccagctagat atttttgagg aagacaatca taaaaaatat
     2941 aaagtctaca ttcgagaaac gtcttcttac tttgtctata ataaagaaaa catggataat
     3001 aattgtttca tcaagggtag gaccttaatc agacaattga gcaacgatag ccaaaaactc
     3061 ccctatagac gaccaacttt aaaaagtctt aaagagaaga tttctgaaat taatcttatg
     3121 attgaactat ctaacacgaa caaacaatac caagaaatca aagatgagct tgtattagaa
     3181 attgcagaga ttgatatgca gctagaagaa acccaagaaa aaattgccac cttaaacaag
     3241 atggcggaag tccttatcaa tttgaagagt gaagatcatg agactagaaa attagctaag
     3301 tatgattttg accaaatgaa catgacggaa tcaataatgt tagatcgact caatacggat
     3361 attttaaaat tacaacagga acttggtaat gaaattaata aatatgag
//
LOCUS       DAVMEI010000155         3371 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVMEI010000155 DAVMEI010000000
VERSION     DAVMEI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.155"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..2738
                     /gene="polA"
                     /locus_tag="VYQ24_001768"
     CDS             105..2738
                     /gene="polA"
                     /locus_tag="VYQ24_001768"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1968872.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEF
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKSYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2823..3260
                     /locus_tag="VYQ24_001769"
     CDS             2823..3260
                     /locus_tag="VYQ24_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1968873.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          912 a          594 c          865 g         1000 t
ORIGIN      
        1 actaaattct ctggctctat ctatttattc ggaaaaagtt tgaaaaatac ttgctctagc
       61 tcttcccaat ggtatttttt gattctttcc tttataatgg gtgtatggat aagaaaaaat
      121 tattattgat tgatgggtct tctgtagctt ttcgggcgtt ttttgcgctg tatcagcagt
      181 tggaccgttt taagaatgcg gctggtttgc ataccaatgc gatttatggt tttcagttga
      241 tgttgagtca tttgttggag cgggttgagc cgagtcatat tttggtggct tttgatgcgg
      301 gaaagacgac cttccggaca gagatgtatg cggactataa gggtggtcgg gccaagactc
      361 ctgatgagtt tcgtgagcaa tttcctttca ttcgtgagtt gctggatcat atggggattc
      421 gtcactatga gctggctcag tatgaggcgg atgacatcat tgggacgctg gataagctag
      481 cagagcagga tggttttgat attaccattg tcagtgggga caaggatttg attcagctga
      541 cggatgagca tacggtggtt gaaatttcca agaaaggtgt ggctgagttt gaggccttta
      601 cgccagatta cctcatggaa gaaatgggcc tcacaccagc tcagtttatc gatctcaagg
      661 cgctcatggg tgataagtcg gataatatcc ctggggtgac caaagtcggt gaaaagacgg
      721 gtattaagct cttgctggag catggttcgc ttgaggggat ttatgaaaat attgatggaa
      781 tgaagacttc taagatgaag gaaaatctca tcaatgacaa ggaacaggcc tttttgtcta
      841 aaacactagc gaccattgat accaaggcac cgattgcgat tggtttagag gacttggtct
      901 atagtggtcc agatgttgaa aatcttggga aattctatga tgagatgggc ttcaaacagc
      961 tcaagcaggc tttaaatgtg tcgtcagctg atgtgtctga gagtttggat tttactattg
     1021 ttgaccaaat cagtcaagat atgctgagtg aagagtctat cttccacttt gagctttttg
     1081 gtgagaatta ccatacggat aatttggttg gatttgcctg gtcttgtggg gataagctct
     1141 atgccacaga caagcttgag ctgttgcaag acccgatttt caaggatttc ttagaaaaaa
     1201 catctctgag agtttatgac tttaagaagg ttaaagttct tttgcaacgt tttggtgtgg
     1261 atttgcaggc gcctgctttt gatatccgtt tggctaaata cctcctttcg actgtggagg
     1321 acaatgaaat tgcgaccatc gctagtcttt atggtcagac ttacttggtt gatgatgaaa
     1381 ctttctacgg taagggtgtt aaaaaggcca ttcctgaacg tgagaaattc ttggaacact
     1441 tagcttgtaa acttgctgtt ttggtagaaa cagagcctat tttacttgaa aaactcagcg
     1501 aaaatgggca attagagctt ctttatgata tggagcaacc tctggctttt gtccttgcca
     1561 agatggaaat tgctgggatt atggtcaaga aagagacctt gcttgagatg caggctgaaa
     1621 atgagtttgt cattgaaaaa ctgactcaag agatttacga gctggccggt gaggagttta
     1681 atgtcaactc gcctaagcag ttgggcgtgc ttctctttga gaaattggga cttcctctag
     1741 aatacactaa gaaaaccaag acaggttatt cgacagcagt ggatgttttg gagcgtctcg
     1801 ctcctattgc tccgattgtt aagaaaatcc tggattaccg tcaaattgct aagattcaat
     1861 ctacttatgt aattggcttg caggactgga ttttggctga tggaaagatt catactcgct
     1921 atgtgcagga tttgacccag accgggcgtt tgtctagtgt ggatccaaac ttgcaaaata
     1981 ttcctgcccg attggaacaa gggcgcttga ttcggaaggc ttttgtgcca gagtgggagg
     2041 atagtgtgct actcagctct gattattcac agattgaatt gcgcgttttg gcgcatattt
     2101 ctaaggatga gcacttgatt aaggccttcc aagagggggc agatatccat acttcgacag
     2161 ccatgcgggt ctttggcatt gagcgtcctg atgatgtgac tgcaaacgac cgtcgcaatg
     2221 ccaaggcagt taactttgga gtggtttatg ggatttcaga ctttggcttg tctaataatt
     2281 tgggaattag tcgtaaggaa gccaaagcct acattgatac ctactttgaa cgttttccag
     2341 gtattaaaaa ctacatggat gaagtggtgc gggaggcgcg tgataagagc tatgtagaga
     2401 ccctctttaa gcgtcgccgt gagttgccag atatcaattc gcgcaacttc aatattcgtg
     2461 gttttgcgga gcgaactgct atcaactcac ctatccaggg ttcggcagca gatattctca
     2521 agattgccat gattcagctg gataaagcct tggttgcagg tggttatcag accaagatgc
     2581 tgttacaagt gcacgatgaa atcgtccttg aagtgcctaa atctgaattg gtagagatga
     2641 aaaaattggt gaaacaaacc atggaagaag ccattcaact cagtgttcct cttatcgcag
     2701 atgagaatga aggggcaacc tggtacgagg ctaaataaaa agggggctag tcctcctttt
     2761 ttgtagtaga attctgcaaa ccttttcaaa atgtgctata ctgatgaaaa aggaggattt
     2821 ctatgagtca agaatttatc aatccaagtg atggcgtgat tcgtcagtat ctcgcaacga
     2881 gtaaaaccct tgctgtggtg ggtttgtctg accgtgaaga aacaaccagc aatcgagtaa
     2941 caaaggaaat gcaggctcgg ggctataaaa tcatcccagt caatcccaag gcggcaggtg
     3001 gcgaaatctt gggtgaaaag gcttatgcta gcctcgctga aattcctttt cctgtagata
     3061 ttgtcaatgt ttatcgtcgc agtgaatttc tgcccgatgt ggcgcgtgat tttctcaaag
     3121 ctgatgccaa gattttttgg gcacagctag gacttgaaag tttagaagcg aaagagattt
     3181 tgcgtgatgg tggatgtgat gatatcgtga tgaatcgctg tatcaagaga gaacatactc
     3241 gcttgattga ggaagcataa gaaaaaggta gctgatgggc taccttttgt gttatactca
     3301 atgaaaatca aagagcaaac taggaaacta gctgcgggtt gctcaaaaca ctgttttgag
     3361 gttgtagata c
//
LOCUS       DAVMEI010000156         3310 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVMEI010000156 DAVMEI010000000
VERSION     DAVMEI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.156"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(219..830)
                     /gene="rpsD"
                     /locus_tag="VYQ24_001770"
     CDS             complement(219..830)
                     /gene="rpsD"
                     /locus_tag="VYQ24_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1968874.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1025..2077)
                     /locus_tag="VYQ24_001771"
     CDS             complement(1025..2077)
                     /locus_tag="VYQ24_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1968875.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2074..2772)
                     /locus_tag="VYQ24_001772"
     CDS             complement(2074..2772)
                     /locus_tag="VYQ24_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1968876.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2939..>3310)
                     /locus_tag="VYQ24_001773"
     CDS             complement(2939..>3310)
                     /locus_tag="VYQ24_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1968877.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT          926 a          743 c          631 g         1010 t
ORIGIN      
        1 aactttttta cgaaaatcta ttgaatatgc cataaaagga ttataccaca ttgtgtacta
       61 tttttggttc attttactat atcactttgg actatatcgg tctagttatg tctcaacgat
      121 tatagctgga tactaagaga aatcaaaaaa gcactaaggg agaacgtccc aaagtgctta
      181 atatcaaggc ctcaaagtct atcttattca ataaaatatt acaacatttt gttgtagaat
      241 tcaacgacaa gtgcttcgtt gatttctggg ttgatttcgt cgcgttctgg caagcgagtc
      301 aatgaacctt ccaacttttc agcgtcgaat gatacgaatg ctggacgtcc aagagtagct
      361 tctactgctt caaggattgc tggaactttc aatgattttt cacgaactga gatcacttga
      421 cctggagtta cgcggtatga tgggatatca acgcgtttcc cgtcaacaag gatgtgaccg
      481 tggtttacga attgacgagc ttgacgacga gtagtcgcga gaccaagacg gtaaacaacg
      541 ttatccaaac gacgttccaa aagaagcata aagttgaaac ctaggattcc gcctttgatt
      601 tttgtagctt gtacgaacaa gttacggaat tgtttttcac ctacaccgta agtgaaacga
      661 agtttttgtt tttcagccaa ttgcaaaccg tattctgaca atttagaacg gttgtttggt
      721 ccgtgttgtc ctggtacgta gttacgacgt gccaattctt tacctgtacc tgtaagtgaa
      781 aggccaaggc gacgagcttg tttccaagat ggtcctgtat aacgtgacat atgaatgtcc
      841 tcctgatata aataatattt cggtggaaat agtcacttag aaagccctga ttcgtgcaga
      901 tgcccttcgc ctaaacagcc aaggttactt gtcataagac acctgttgac gagcttcatg
      961 ctttcctgct gatatttcac acaaaatcta ttctaccatg aatagctgga tttgtaaagg
     1021 ggttttaggc tttattttca ctaccagaga ggttgagaac gagggtaaag gtacttccta
     1081 gaccgtactg gctgctgact gtgatttccc cacccaattg atgggccaat tcacgcgcaa
     1141 tcgcaagtcc taatccatga ccacctgtct tcatgttacg cgaagtttcg acacgataaa
     1201 gacgtttgaa aatattttcc aaatcctctg gggcaatacc ctgcccttca tcggtcacac
     1261 tgattgaaag ctggtccttc tccagcttag ccaccacttc cagcttggtt cctggagcag
     1321 aatatttaaa agcgttatcg accagattca ccaagatacg agaaagctta gcataatctc
     1381 cctcaatccg ggcagactct gggattacct gcaagtggac atctcttctc tcctgctcaa
     1441 tcaaaaactg aaattcactc atgcactcaa ttaagagctt gtccagaaaa atactgtctt
     1501 tactggtagt ttccacctga ttcctagctg tgtttagggt caaaaaattc aactcctcaa
     1561 ccagtttatt gagcctctcc gtctggcgtc caatggttgc tagataatga gcttgctccg
     1621 actccttgat aatcccatcc aaaatccctt ctaccgtcgc ttggatcgaa gtgataggag
     1681 tcttaatatc atgcgacaac tgggcaatca tcaagccctt ttctcgttcg ctttcttcca
     1741 aggaatcaaa gcttacctgc aaatcatggg acatctcatt aaaagtttgc cctaattgct
     1801 gaaattctac aggaccttga acctccaaat ttgaaggaaa atccttggcc gctacccgct
     1861 tggcatgctc cttgagtttg cccaacgacg taaagactgg caataggaga aagagactaa
     1921 tcccagcacc gacaaggctg gcaacgatgg tcatcccaag caaaaagtaa atctcgcctt
     1981 tcgcaatcag cattttttga acagcccaaa aaacaaccaa aatggttaag agggttgaaa
     2041 taatatatcc aaccaaaata taacttttta gtttcatgtt tgtcctctcg gtttctctat
     2101 cttatatccc aacccccaaa ctgtcttaat agtgggcgtt tggtcactac tatattttgc
     2161 cagctcctgt cgaagagcat ggatatgcac attcaaggta ttggtgtcat ccacgtagtc
     2221 ttctttccag atcttttcat agaggtctgt cttggagaaa actcgctctg gattgctagc
     2281 taaaatccac agcaattcaa atgatttaac agttaaatcc agcatttctt ctcctatttg
     2341 aacttcatga ctactatgat tcatttttag attgccaagg gaaatcagct ctgtttcgcc
     2401 cccacgatga aggcggcgca aaatattgtg gacacgcaaa accagctcac gtgggctaaa
     2461 aggcttagca ataaaatcat ctgctcccaa gctcaggccg taaatcttgt cctgttcact
     2521 ggtcttagca gtaataaata ggaaaggctg ctctggtgat aagtattgaa cctcactgat
     2581 taaatcataa ccatccatcc gaggcatcat gacatctgtg ataatcaaat caatcggttt
     2641 tttcttgaac agctctagcg cttccagtcc atcatgggct accaagacct gataacctgc
     2701 ctgaattaag tatctctgat gaatatctgt gatttctacc tcgtcgtcaa cgagtaaaat
     2761 tgtctttccc atctgtctct cctttgataa aaacagtgct atactgcttt aagtataaca
     2821 ctatttttat tgttttacga tgattttaat ctgctattga aagaaagtcc tgattgctga
     2881 ttgagttgtt aaactaatct tagtcagccc actatccatc atttatttcc ttagctgatt
     2941 agttttcttc ttttttgttt ttcaaaccta gaccaccgag taaacctgca agcgcaagcc
     3001 caaggaaacc aatacttgcc attgatgttt gagtctcacc agtatttggt agcatagctt
     3061 tatcctctga catcatcgta tcagacatct tgttagcaga agcagccatg ttttcacctg
     3121 ccatcgtgtt ggtagaactt gtcatggtgt cagcaggcat gctatctgta gtacctgtag
     3181 catgattgtg attcatcgga gtcacgccag aaccagagtt agaaggtgat aatgaaccat
     3241 ttgctgtgtc tgatgtttct ttaacattta tcttaatagt gactttttta gttgctacga
     3301 tgttgtccaa
//
LOCUS       DAVMEI010000157         3295 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVMEI010000157 DAVMEI010000000
VERSION     DAVMEI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.157"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            92..3211
                     /gene="phtE"
                     /locus_tag="VYQ24_001774"
     CDS             92..3211
                     /gene="phtE"
                     /locus_tag="VYQ24_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1968878.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSISKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1231 a          542 c          641 g          881 t
ORIGIN      
        1 attaaatggt taaataaccg gtttagaaaa ctatttaata aagtaaaaga agttgagaaa
       61 aaacttcatc atttattgaa atgagggatt tatgaaattt agtaaaaaat atatagcagc
      121 tggatcagct gttatcgtat ccttgagtct atgtgcctat gcactaaacc agcatcgttc
      181 gcaggaaaat aaggacaata atcgtgtctc ttatgtggat ggcagccagt caagtcagaa
      241 aagtgaaaac ttgacaccag accaggttag ccagaaagaa ggaattcagg ctgagcaaat
      301 tgtaatcaaa attacagatc agggctatgt aacgtcacac ggtgaccact atcattacta
      361 taatgggaaa gttccttatg atgccctctt tagtgaagaa ctcttgatga aggatccaaa
      421 ctatcaactt aaagacgctg atattgtcaa tgaagtcaag ggtggttata tcatcaaggt
      481 cgatggaaaa tattatgtct acctgaaaga tgcagctcat gctgataatg ttcgaactaa
      541 agatgaaatc aatcgtcaaa aacaagaaca tgtcaaagat aatgagaagg ttaactctaa
      601 tgttgctgta gcaaggtctc agggacgata tacgacaaat gatggttatg tctttaatcc
      661 agctgatatt atcgaagata cgggtaatgc ttatatcgtt cctcatggag gtcactatca
      721 ctacattccc aaaagcgatt tatctgctag tgaattagca gcagctaaag cacatctggc
      781 tggaaaaaat atgcaaccga gtcagttaag ctattcttca acagctagtg acaataacac
      841 gcaatctgta gcaaaaggat caattagcaa gccagcaaat aaatctgaaa atctccagag
      901 tcttttgaag gaactctatg attcacctag cgcccaacgt tacagtgaat cagatggcct
      961 ggtctttgac cctgctaaga ttatcagtcg tacaccaaat ggagttgcga ttccgcatgg
     1021 cgaccattac cactttattc cttacagcaa gctttctgcc ttagaagaaa agattgccag
     1081 aatggtgcct atcagtggaa ctggttctac agtttctaca aatgcaaaac ctaatgaagt
     1141 agtgtctagt ctaggcagtc tttcaagcaa tccttcttct ttaacgacaa gtaaggagct
     1201 ctcttcagca tctgatggtt atatttttaa tccaaaagat atcgttgaag aaacggctac
     1261 agcttatatt gtaagacatg gtgatcattt ccattacatt ccaaaatcaa atcaaattgg
     1321 gcaaccgact cttccaaaca atagtctagc aacaccttct ccatctcttc caatcaatcc
     1381 aggaacttca catgagaaac atgaagaaga tggatacgga tttgatgcta atcgtattat
     1441 cgctgaagat gaatcaggtt ttgtcatgag tcacggagac cacaatcatt atttcttcaa
     1501 gaaggacttg acagaagagc aaattaaggc tgcgcaaaaa catttagagg aagttaaaac
     1561 tagtcataat ggattagatt ctttgtcatc tcatgaacag gattatccaa gtaatgccaa
     1621 agaaatgaaa gatttagata aaaaaatcga agaaaaaatt gctggcatta tgaaacaata
     1681 tggtgtcaaa cgtgaaagta ttgtcgtgaa taaagaaaaa aatgcgatta tttatccgca
     1741 tggagatcac catcatgcag atccgattga tgaacataaa ccggttggaa ttggtcattc
     1801 tcacagtaac tatgaactgt ttaaacccga agaaggagtt gctaaaaaag aagggaataa
     1861 agtttatact ggagaagaat taacgaatgt tgttaatttg ttaaaaaata gtacgtttaa
     1921 taatcaaaac tttactctag ccaatggtca aaaacgcgtt tcttttagtt ttccgcctga
     1981 attggagaaa aaattaggta tcaatatgct agtaaaatta ataacaccag atggaaaagt
     2041 attggagaaa gtatctggta aagtatttgg agaaggagta gggaatattg caaactttga
     2101 attagatcaa ccttatttac caggacaaac atttaagtat actatcgctt caaaagatta
     2161 tccagaagta agttatgatg gtacatttac agttccaacc tctttagctt acaaaatggc
     2221 cagtcaaacg attttctatc ctttccatgc aggggatact tatttaagag tgaaccctca
     2281 atttgcagtg cctaaaggaa ctgatgcttt agtcagagtg tttgatgaat ttcatggaaa
     2341 tgcttattta gaaaataact ataaagttgg tgaaatcaaa ttaccgattc cgaaattaaa
     2401 ccaaggaaca accagaacgg ccggaaataa aattcctgta accttcatgg caaatgctta
     2461 tttggacaat caatcgactt atattgtgga agtacctatc ttggaaaaag aaaatcaaac
     2521 tgataaacca agtattctac cacaatttaa aaggaataaa gcacaagaaa actcaaaact
     2581 tgatgaaaag gtagaagaac caaagactag tgagaaggta gaaaaagaaa aactttctga
     2641 aactgggaat agtactagta attcaacgtt agaagaagtt cctacagtgg atcctgtaca
     2701 agaaaaagta gcaaaatttg ctgaaagtta tgggatgaag ctagaaaatg tcttgtttaa
     2761 tatggacgga acaattgaat tatatttacc atcaggagaa gtcattaaaa agaatatggc
     2821 agattttaca ggagaagcac ctcaaggaaa tggtgaaaat aaaccatctg aaaatggaaa
     2881 agtatctact ggaacagttg agaaccaacc aacagaaaat aaaccagcag attctttacc
     2941 agaggcacca aacaaaaaac ctgtaaaacc agaaaactca acggataatg gaatgttgaa
     3001 tccagaaggg aatgtgggga gtgaccctat gttagatcca gcattagagg aagctccagc
     3061 agtagatcct gtacaagaaa aattagaaaa atttacagct agttacggat taggcttaga
     3121 tagtgttata ttcaatatgg atggaacgat tgaattaaga ttgccaagtg gagaagtgat
     3181 aaaaaagaat ttatctgatc tcatagcgta aggaatagca gtagaaaaag tctgaatcaa
     3241 aaatgaagtt ctctcaaaag ttagaaataa aactctgact ttgggagaat ttcat
//
LOCUS       DAVMEI010000158         3282 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVMEI010000158 DAVMEI010000000
VERSION     DAVMEI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.158"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            120..1631
                     /locus_tag="VYQ24_001775"
     CDS             120..1631
                     /locus_tag="VYQ24_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1968879.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1653..2984
                     /locus_tag="VYQ24_001776"
     CDS             1653..2984
                     /locus_tag="VYQ24_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1968880.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICQDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLYGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAIYRLAT"
BASE COUNT          898 a          679 c          702 g         1003 t
ORIGIN      
        1 actagttttt tagactttaa tatggtaaaa caaatataga tggaaagcgt tttcttaaat
       61 gtttatttaa gaaagtagtt ggttttttac actttgttag acatcaggag gaaaaacaaa
      121 tgagtgaaaa agctaaaaaa gggtttaaga tgccttcatc ttacaccgta ttattgataa
      181 tcattgctat tatggcagtg ctaacttggt ttatccctgc gggggccttt atagaaggta
      241 tttacgagac tcagcctcaa aatccacaag ggatttggga tgtcctcatg gcaccgattc
      301 gggctatgct aggtactcat ccagaggaag gttcgctcat taaagaaacg agcgcagcga
      361 ttgatgtagc cttcttcatc cttatggttg gtggtttcct tggcattgtc aacaaaactg
      421 gtgctcttga cgtagggatt gcctctatcg tgaagaagta taagggccgc gaaaaaatgt
      481 taattttggt attgatgcct ttgtttgccc tcggtggtac aacttatggt atgggtgaag
      541 aaacaatggc cttctatcca ctccttgtgc cagttatgat ggccgttggt tttgatagcc
      601 tgactggtgt tgcaattatt ttgctcggtt ctcaaatcgg ctgtttggca tctactctga
      661 atccatttgc gacaggtatt gcttcagcga ctgcgggagt tggtacaggg gacggtatcg
      721 tacttcgtct gatcttctgg gttaccttga ctgctcttag tacttggttt gtttaccgtt
      781 atgcggataa gattcaaaaa gatccgacta agtcactggt ttatagtact cgcaaagaag
      841 atttgaaaca ctttaacgta gaagaatctt catctgtaga atctacactt agcagcaaac
      901 aaaaatcagt tctcttctta tttgtgttga cattcatctt gatggtattg agcttcattc
      961 catggacaga ccttggcgtt accatttttg atgactttaa tgcttggttg actggtcttc
     1021 cagttattgg taatattgtc ggttcatcta cttctgcact aggtacttgg tacttcccag
     1081 aaggcgccat gctctttgcc tttatgggta tcctgattgg tgttatttat ggtcttaaag
     1141 aagataagat tatctcttcc ttcatgaatg gtgctgctga cttgctcagt gttgccttga
     1201 tcgtagcgat tgctcgtggt attcaagtta tcatgaacga cggtatgatt accgatacaa
     1261 tcctcaactg gggtaaagaa ggcttgagcg gtctatcttc acaagtcttt atcgttgtaa
     1321 cttatatctt ctatctacct atgtcattct tgatcccatc ttcatctggt cttgccagcg
     1381 caactatggg tatcatggct ccacttggag aatttgtaaa tgtccgtcct agcttgatta
     1441 tcactgctta ccaatctgct tcaggtgtct tgaacttgat tgcaccaaca tctggtattg
     1501 tgatgggagc tcttgcactt ggacgtatca acattggtac ttggtggaaa ttcatgggca
     1561 aactcgtagt cgctattatt gtagtgacca tcgcccttct tctccttgga accttccttc
     1621 cattcctata aaatagtgag tgaggtgatt ccatgaaaat agatataaca aatcaagtta
     1681 aagatgaatt tcttatatca ttaaaaacct tgatttccta tccttcagta ctcaatgaag
     1741 gagaaaatgg aacacctttt ggacaagcaa tccaagatgt cctagaaaaa actttagaga
     1801 tttgtcaaga cataggtttc actacctatc ttgaccctaa aggttattac ggatatgcag
     1861 aaatcggtca gggagcagag cttctggcca ttctctgtca tttggatgtt gttccatcag
     1921 gtgatgaagc agattggcag acaccgccat ttgaagcaac tatcaaagac ggctgggtat
     1981 tcggacgtgg tgtccaagat gataaaggcc cttcgctcgc agctctctat gcagtaaaaa
     2041 gcttgctgga ccaaggtatt cagttcaaaa agcgcgtacg ctttatcttt ggtaccgatg
     2101 aggaaaccct ctggcgctgc atggcacgct acaataccat cgaagaacag gccagcatgg
     2161 gctttgcacc tgactcatct tttcctctga cctatgctga aaaagggctt ctacaggtca
     2221 aactttatgg ccctggatcg gatcaactag agcttgaagt aggaggcgcc tttaacgttg
     2281 taccagacaa ggccaactac caaggtcccc tctatgaaca ggtttgtaac ggtctcaaag
     2341 aagctggtta tgattaccaa accactgaac aaaccgtaac ggttctcgga gtgccaaagc
     2401 atgctaagga tgctagtcaa ggtatcaatg ctgtcatccg actagctacc attcttgctc
     2461 ctctccaaga acaccctgct ctcagttttc ttgcaacaca agcaggtcaa gacggcacag
     2521 gaagacaaat ctttggtgat atagcagatg aaccttctgg tcacctatcc tttaatgtcg
     2581 caggtctcat gatcaatcat gaacgttctg aaatccgtat tgacattcgg actcctgtct
     2641 tagctgacaa ggaagaacta gtagagttgc ttacaagatg tgcacaaaac taccaactcc
     2701 gctacgaaga gtttgactat ctagcgcctc tatacgtcgc aaaagacagt aaactcgtta
     2761 gcacactgat gcaaatctac caagaaaaga ctggcgataa cagtcctgct atttcatccg
     2821 gtggtgccac ttttgctcgc accatgccaa attgtgtagc cttcggcgcc ttattcccag
     2881 gagcgaagca gacagaacat caggcaaatg aatgtgccgt tctagaagat ttgtaccgtg
     2941 ctatggatat ttatgccgaa gccatctatc gacttgcaac ttaatcaggc aactgtttct
     3001 acaaaaaaaa atcgaccgat taatgaactg caccccaaaa gttagacaga ataaatctaa
     3061 cttttggggt gttttattat gaaattgaat tatgaagata aagttcagat ctatgaacta
     3121 agaaagcaag gacaaagctt caaacagctt tcaaaaagat ttggtgtgga tgtttctggt
     3181 ctaaagtcat ctgaatcttt gagatgagct ttataaatcg cttttttcag tttttgcact
     3241 ggtgtttcga taaactcaaa ctttttagcc gtggtattgc ct
//
LOCUS       DAVMEI010000159         3272 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVMEI010000159 DAVMEI010000000
VERSION     DAVMEI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.159"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(3..968)
                     /locus_tag="VYQ24_001777"
     CDS             complement(3..968)
                     /locus_tag="VYQ24_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1968881.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VYQ24_001778"
     CDS             complement(1086..1499)
                     /locus_tag="VYQ24_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1968882.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VYQ24_001779"
     CDS             complement(1496..1732)
                     /locus_tag="VYQ24_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1968883.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(1792..2853)
                     /locus_tag="VYQ24_001780"
     CDS             complement(1792..2853)
                     /locus_tag="VYQ24_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1968884.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYG
                     GNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVF
                     LLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVALVDKSQLAIQ
                     KSLEELETLKKSLMQEYFG"
     gene            complement(2850..>3272)
                     /locus_tag="VYQ24_001781"
     CDS             complement(2850..>3272)
                     /locus_tag="VYQ24_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1968885.1"
                     /translation="NNLKFTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTIT
                     VLKKNERYKEKIISDYLGVFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQ
                     ENIICILNTIKGLITKRKLQLDELNLLVKSRYSEEVVA"
BASE COUNT          979 a          638 c          466 g         1189 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagtctc
      241 tgggcttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtccgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctggta ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaacccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga gacttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcagata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
     1861 caattgtgat ttgtcgacca gggctacaaa gtctgcgaac tcattttgga gggctagagg
     1921 ggggaggata tatttctgat tttctaatgg tttttgagtt atctttggtt gacctgagaa
     1981 atcagcaaat ttactaaaat caattacttt cattaattct aataaaaata caagattgaa
     2041 atcggaattc ttaaattctt ttatatatat agcattgtca cttatccata ccttaccgtg
     2101 ggtagttctt acattaccac aatatgcacc tacacgacca atgatgatag taggattatc
     2161 aattaaatat tttcgacttt tccaagcaat gccatttcca ccataggcag gataaccttc
     2221 aaaaactcta tcatgtttat caagaaattt tccacttgaa aatttaaaac actgttgtcc
     2281 cgtttttact gcaaatttct tgttattatt taaaggatcc ccaaacatct cgttaaatcg
     2341 ggattttaca agtagattaa gctcttcaag ttgttcttgt ctccttaaaa tcagcttgct
     2401 taacaaatcc agctcacttg caattctttg ttgctctccc aaatttgtat atggaactat
     2461 aatattatca aaatattttt tagttaaatg tttcattgtg gaaccatgag tatgttttac
     2521 agcatcttgt aaaccttttt caacaacata tttaaaatac gatttatcta tatctatctt
     2581 atcaaaaacc actttaaaaa tgtgctgatt taatactgcg gatcttccac accactgaaa
     2641 aacccctaat gtacccgacc acgaaataag aatatcacca gcttcaacaa tgtatttttt
     2701 atcaatcgta cccgaataat aatttatccc ttttgaagtt ttagtcagat tctgtattct
     2761 gataatctct ttcccttcag atgaccaatc ttgaggttta aaagcatagc cattgataaa
     2821 agtagccact tgccctaatt tcactttctt catgcaacca cctcctcgga atatcgggat
     2881 ttgacgagca agtttagttc atctaactgc aattttcttt tagtaataag ccctttaatc
     2941 gtattaagaa tacagataat gttctcttgt tcttcgatac ctagcaattc taattgtaaa
     3001 tcaagtaata tattcttgtt taaatgagga attgttgcac ctgttgaatg ttctcgtaaa
     3061 tactgcgatt tactttccaa aaagactccc aagtaatctg atataatttt ttctttgtat
     3121 cgctcattct tttttaagac cgtaattgta ctaccaacag ctcccgataa tccataacca
     3181 actgttcctg cattagctcc atcccatgct atcagaatat catctgggag tgcttcagtc
     3241 atatttaaac tttcagtgaa ttttaaatta tt
//
LOCUS       DAVMEI010000160         3195 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVMEI010000160 DAVMEI010000000
VERSION     DAVMEI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.160"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     regulatory      complement(154..242)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(451..1080)
                     /locus_tag="VYQ24_001782"
     CDS             complement(451..1080)
                     /locus_tag="VYQ24_001782"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1968886.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(1082..1864)
                     /gene="thiM"
                     /locus_tag="VYQ24_001783"
     CDS             complement(1082..1864)
                     /gene="thiM"
                     /locus_tag="VYQ24_001783"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1968887.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALSVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(2153..2821)
                     /locus_tag="VYQ24_001784"
     CDS             complement(2153..2821)
                     /locus_tag="VYQ24_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1968888.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLIMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(3036..3124)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT          991 a          680 c          455 g         1069 t
ORIGIN      
        1 tagaaattaa tttgaatttt ctaattgatt tgttcgcatc ttatttcaat ctactatatc
       61 atcttcatcc agtttcgtaa aagaaaaaac tctaattaca gatacgaatt agagttcagc
      121 ttacaagatt agacagttct tttcgacata cgaaaaaaac atttcacatt tcccttcgcc
      181 agtcttaact gtatcaggtt caatgggtat catctcagcc taaagcaccc caaatgcctt
      241 tattatttaa ttatgtgatt attataacac acattttata ctagttcaag aaattgaact
      301 ggaaatacag ccttgcactc acaaagacag cagatctttc ttttgcaaaa aacaaatgac
      361 ctgtttgatg aattagccat tcaagctgaa tctagacata gctttttaaa aaaggaaaat
      421 cctacttact tagaatccaa ggatagatat ctattgttca ctcatttccc gaacagtttt
      481 ttctatattt tttgcatacg atattgccga aatgattgaa acgccgtcaa cattggtctt
      541 tataatgtct ttaatatgtt tcgtctgtat cccaccaatt gcaactaaag gcatttgtgg
      601 caatagtttt ctcatcaatt caagaccttc ataacctata gcaccaccag catcatcttt
      661 tgactgggta tcaaatacag gcccaacacc aacataatct acatattcaa cttttgattg
      721 ttgaaattct tcctcgtttt ttatagaaag accaattatt ttatctggca tcaattttct
      781 aatttcatca acaccaatat catcttgacc tacatgtacg ccatcagcgt caatttccat
      841 tgctaaatct atatcgtcat taacgataaa tggaacattg tattttttac aaagttcttt
      901 aatttggata gctagctcaa gtttttctaa gccttctaaa gcaccctcac ctttttctcg
      961 aaattgaaat aaggttatac caccttttaa ggcttcctca acgactgtat atagattttt
     1021 tccttggcaa gtagtcgttc cacaaataaa atatagtttt agtaattctt tatgaaacat
     1081 cttacttcac tcttttgaat tcctttacat cttcatctgt aatctcgtat aaggcattta
     1141 taaattcaac tttaaatgtc ccaggaagat gtccatttgg acgtttttct gccatttctc
     1201 cagcgatatt gtaaaccaac attgctgttt ctaatgattt caattcttga cctttttcta
     1261 gtccgataaa gcttgctact acagctccta ataagcatcc tgtcccaatg actttcggca
     1321 tcatagcact accattatga atcgttacca cttctccatt aacagcaatg gcatccactt
     1381 cacctgttac tactattgga atattgaact tctcatttgc tgctagagca atttcgtcaa
     1441 tattatctac gcccgcacta tctactcctt tagatgccac atctattcct actaaagagg
     1501 caatctcgcc agcatttcct ctaatcgctg ctagtttata attgttgatt agatcatctg
     1561 ctactttttt tctatattct cctgctccac aggctacagg atctaaaact gctgggacat
     1621 tatatttctc cgcaattttc agagcagctt ggtataattt ccaattttca tctgtcaatg
     1681 ttcctatgtt tattaataaa ccaccagcat actttaacaa atcctctaaa tctgctggaa
     1741 actcactcat ggctggtgag acgctcagtg ctactaatcc atttgctgtg aaatttttta
     1801 ctacatcatt ggtgatacaa atgaccaatg gtgctttttc ttttaataat tttaaacttg
     1861 tcatattgaa atccttcctt tacactttat acgatctact gatttcgatt tatctctatc
     1921 tttcgttaag aaattttttt catttacgtt gaatgattta tactcaatga aaatcaaaga
     1981 gcaaactagg aggctaaccg caggttgctc aaaacactac tttgagcctg tagataagac
     2041 tgacgaagtc agctcaaaac actgttttga ggttgtggat agaactgacg tggtttgaag
     2101 agattttcga agagtcttac ctcatcaaat ttgtaaatat catgagcctt ctctagacat
     2161 cgtaaccaat atcaaaaaaa gctaattcta aagcgactgc ttgattccag cgttgctgaa
     2221 gttctgtcaa atcttctcga tttttaccga cacgattgag ttcgtcaacc agaaattgaa
     2281 cccactctgc aaagaaagga cctctgtgga gattgatcca ttccgaatga atatagactt
     2341 caggtagagc caaatcttta gaaccccagt ctaaatagag accttctgca atgaccagca
     2401 taatcaaaag atgggcatag tctgatgaag ccactgccga atacattaga tcctgaaagg
     2461 cttttgttac agggtgcaaa gtcacttcta gatagtcatt ctctgctact tttaactctt
     2521 taaaagcctt ttggaaataa ccatcttcat ctgcttcaag aaagcctagt tgcttggcaa
     2581 aacgaagctt ggattcaagt ttatctgcgt gagctacgca ggcacccagc atggataaga
     2641 aggcatcaaa gaagtgataa tcttgaatca gatagtcctt taagacctta ttctcaattg
     2701 tccccgcaaa aagttcctta acaaaacgat gattgattgc agcctgccaa tccttctgac
     2761 tgctttttaa taattctcca acagtcaaac ctggctgaaa tgcatagtct tgtgtttcca
     2821 tatttacttc tcctctcttt acttgttagt aattaataaa acaccaagaa atatcaagca
     2881 aaatcgtaat tccacttgat ccttttaaag cacatcgaga gcatttgcag agagctaact
     2941 aaacaagcct atccagttta tataaacaaa aaactccaat tacaatcaag aattagagtt
     3001 gacttacaag attagatcgt tcatttcgtc atacgaaaaa acgtttcaca tttcccttcg
     3061 ccagtcttaa ctgtatcagg ttcaatgggt atcatctcag cttaaagcac cccaaatgtc
     3121 tctattattt aattactgaa ccagtatagc aaaaaatgaa agccctagca agatatttga
     3181 ccgaaaaata tcttt
//
LOCUS       DAVMEI010000161         3188 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVMEI010000161 DAVMEI010000000
VERSION     DAVMEI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.161"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..201
                     /locus_tag="VYQ24_001785"
     CDS             <1..201
                     /locus_tag="VYQ24_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV1968889.1"
                     /translation="KWVNREYRQLFTKNQFHQAMKYAKVNDLSTVTYEQVLSIFNSYL
                     LFNGRKFYLLLIILIRLFAMSC"
     gene            141..542
                     /locus_tag="VYQ24_001786"
                     /pseudo
     CDS             141..542
                     /locus_tag="VYQ24_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            539..1081
                     /gene="sat4"
                     /locus_tag="VYQ24_001787"
     CDS             539..1081
                     /gene="sat4"
                     /locus_tag="VYQ24_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEV1968890.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1174..1968
                     /locus_tag="VYQ24_001788"
                     /note="aph(3')-IIIa"
     CDS             1174..1968
                     /locus_tag="VYQ24_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEV1968891.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2244..2817
                     /locus_tag="VYQ24_001789"
                     /pseudo
     CDS             2244..2817
                     /locus_tag="VYQ24_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            3132..>3188
                     /locus_tag="VYQ24_001790"
     CDS             3132..>3188
                     /locus_tag="VYQ24_001790"
                     /inference="COORDINATES: protein motif:HMM:NF019384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEV1968892.1"
                     /translation="MIVGNLGAQKEKRNDTPIS"
BASE COUNT         1099 a          499 c          725 g          865 t
ORIGIN      
        1 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
       61 aaatacgcca aagtaaacga tttaagtacc gttacttatg agcaagtatt gtctattttt
      121 aatagttatc tattatttaa cgggaggaaa ttttatttgc tattgatcat tttaatcaga
      181 ttgttcgcca tgagctgctg agaatgatat catggaaggt cggcatcgaa acaggcttta
      241 aattaagtgt aggcaagaac tataagttta ttgaaaggta tatatccgag gatttgtggg
      301 agaaactttt gtccacctac cggatggatt cctatgaaaa catatgggaa gcattatttc
      361 tatgccatca attgttcagg gcggtatccg gtgaggtggc ggaaaggctt cattatgcct
      421 atccggagta tgataggaat ataacaaaat ataccaggga catgtataaa aaatacactg
      481 gtaaaaccgg ctgcctggat agcacatatg ccgctgatat agaagagagg cgggaacagt
      541 gattacagaa atgaaagcag ggcacctgaa agatatcgat aaacccagcg aaccatttga
      601 ggtgataggt aagattatac cgaggtatga aaacgagaat tggaccttta cagaattact
      661 ctatgaagcg ccatatttaa aaagctacca agacgaagag gatgaagagg atgaggaggc
      721 agattgcctt gaatatattg acaatactga taagataata tatctttact accaagacga
      781 taaatgcgtc ggaaaagtta aactgcgaaa aaattggaac cggtacgctt atatagaaga
      841 tatcgccgta tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc
      901 tatagaatgg gcaaagcata aaaacttgca tggactaatg cttgaaaccc aggacaataa
      961 ccttatagct tgtaaattct atcataattg tggtttcaaa atcggctccg tcgatactat
     1021 gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta
     1081 gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt
     1141 taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa
     1201 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
     1261 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
     1321 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
     1381 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
     1441 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
     1501 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
     1561 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
     1621 gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc
     1681 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
     1741 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
     1801 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg
     1861 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
     1921 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta cctagattta
     1981 gatgtctaaa aagctttaac tacaagcttt ttagacatct aatcttttct gaagtacatc
     2041 cgcaactgtc catactctga tgttttatat cttttctaaa agttcgctag atagagttct
     2101 atattagaga tgtaaagagt tttggtgaag aggtttgtga gtatggcttt tataaccagg
     2161 gtgcaatgag aagcactaac agcagctagc ttaagaacgc ataggatata ctttttatgg
     2221 aagtcacaga ggaggaataa aatttgaaca ggattaacag agtaacctct attcttattc
     2281 agctgcaatc aaaaaaaaat aattcctgcc aaagaaattg cacagcgatt taatataagc
     2341 ttaaggacag tttacagaga tatccggaca cttgaagaag ccggaatacc aattggatct
     2401 gaggccggaa aaggatattt tcttgtagag ggcttcctac ttccgccggt aatgtttaca
     2461 gcggcggaag tgggtgcatt gattacagca gggaaatttt taaattgcca tggagatgaa
     2521 tcatttataa aggattttga ttcagctatg tataaaatca aatctatttt gaagcatggt
     2581 gaaaaaaact atgcacagga gttggagaac agtattaacg tgtacagcac atctggacag
     2641 aaaaatacat tggcggataa cgttattgca gcaattcaga ctgcaatctg caataaaagg
     2701 gtaatatcaa ttcaatatcc tgcatcagga gggcaagaac cggaaagcag aatgatagag
     2761 cctatatcac tggggtttta tgaacagaac tggtacttaa tcggttttgc aggctaagaa
     2821 atgaatatag gaattttcgt gtggatcgga ttaaaagcat ttgcatagaa gaagagctct
     2881 gtcaaagcca tgatggctct ttggaacaaa tattaaaaca aatgctttca tataaaaaac
     2941 tatatgccct acaatgccgt tgtaaaaatt ggggaaaagg gatatattga ttttatatct
     3001 ttctcaaaat aaatataaaa gccctttaaa gagggctttt atatattaat cacaaatcac
     3061 ttatcacaaa tcacaagtga tttgtgattg ttgatgataa aataagaata agaagaaata
     3121 gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga aatgatacac
     3181 caatcagt
//
LOCUS       DAVMEI010000162         3146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVMEI010000162 DAVMEI010000000
VERSION     DAVMEI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.162"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..737)
                     /locus_tag="VYQ24_001791"
     CDS             complement(<1..737)
                     /locus_tag="VYQ24_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1968893.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMG"
     gene            complement(752..1600)
                     /locus_tag="VYQ24_001792"
     CDS             complement(752..1600)
                     /locus_tag="VYQ24_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1968894.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIANTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHSAGAPWWLQVPNVCWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(1597..3072)
                     /locus_tag="VYQ24_001793"
     CDS             complement(1597..3072)
                     /locus_tag="VYQ24_001793"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1968895.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
BASE COUNT         1090 a          691 c          441 g          924 t
ORIGIN      
        1 cccatgacaa agaataacat ccctttaaaa cagtatctta cccaattttc aggtaagact
       61 tttgatacaa ccgtccccaa tacccctccg catatagaag caagaaaaat aactggaaca
      121 ggtgctaaat gatactgatt taaacctgta accacaacaa ttgtagccaa atgagacagc
      181 tgggaaaaga aaataattgc aagagaatac atcgttgctt cttttatcga aatagaaaaa
      241 aacaccatca ataaagatac attgagcgga ccaccaccaa tccctaaaaa actagaaatc
      301 aagcccaata gaagaccaca aatcatatag aatcctatct tatcaaattg ataagttttt
      361 ttaaaatctg ttaaaacaaa tgatacaagt agcatcacaa atagtaaaat catttgaaca
      421 atggatacta gccgtactga taattgagat aacaaaactt gaaaaataag atgacctaga
      481 aatcctccta aaacagaaga aaaggatacc gtaaaaatca atctccattt tatctcttta
      541 ctttgtgaaa atcttttgat tgttgaaata atcgccatta taaaaacaga gaaactagaa
      601 taaaaagcaa tatcactgac cgaatggtac ccaaaactat ccataagtgg cttaattata
      661 attccaccac ccatgcctga tatagaaccc aatccactag ctattataat gattagaaaa
      721 taaataatct caaccacttt ttaagtccat cctattcttt caaacatcta aaacctagat
      781 ggctactagt ggaagtagaa atacatccat ttctagcagc tactcggtat cgattacaat
      841 aagaacaatg acataaaaag gaacctcctc tgatagcata ctcctcatct tgtatcccat
      901 attttggcaa ctcatagtca tcttcattaa aactagctaa taaggtatat ctcggattgc
      961 gacaccattc ccaaacattc ccaatcattt gatatagacc aaaatcatta ggtaaaaatt
     1021 catagacagg agccgttcct ataaatccat ctaaggcagt attttcataa ggaaatcttc
     1081 cttgccaagt attggcatga taatatccac cttctaaaag agtatctccc caaggatatt
     1141 cagattgtct tcctcctcta gcagcatatt cccactgtgc ttcagtcggt aaagacattc
     1201 cgctccaatt acagaaagcc aatgcatctt ctaaagcaac atgaactact ggatggtctt
     1261 ctaaacccac tagattacta ttctcaccgt agggatgttt ccagcaaacg ttcggaactt
     1321 gtaaccacca cggagcccca gcagaatgag gatatccctc cctttctgcc tctggaacga
     1381 acaatataaa aacaaaagac cactcttgtc gctcagctag tgttttatag cctgtttctt
     1441 tcacaaattg agcaaattct tgattcgtaa ctggagtatt cgcaatggaa aaatccttta
     1501 cactaacagc gacttgagga ccttcaaaat catctaaaaa gccctcttca gaatttgttc
     1561 ccatcataaa tgtcccaccc ttgatttttt tcattttcat gattgactat cccccgtttt
     1621 cgttagagtt ggcgttattt tagaaagttc aacaggaact aactcttcat ccacaacaaa
     1681 accttcttct ctatatctaa gaaagtctac taattttttc ttcatctgac gaacaatagg
     1741 ttggtatttt tcacttggat acaaatcatt catttcatgc ggatcttttt tcatatcaaa
     1801 taactgataa tgatttaaaa caggaaacca gataaatttc cactggtctg tcaagatata
     1861 ttgactagaa tcttttccta aagcatgttc accatgaaat tctgttcgcc acccctcgta
     1921 ctgtccaaat aataaattct taacactccg tccatctagt tcatctgtag tagttcctcc
     1981 tgccaaatcc acgagtgaag gaaaaatatc ttgaattttc actaattgct tgattgttcc
     2041 cctattacca gctatcaatc cagccggatc gtaaataaac gaaggaatgt ggatacttcc
     2101 ctgataagga tatccctttc taaacagata atgttcacca agctgatctc catgatccga
     2161 aacaaaccag ataatcgtat ctttatcatg acgaaattct tttagggcag tcaaaaagcg
     2221 actaatttga tggtctatat gagtaatcaa tccaaaataa gcagcaacca ttcttctttg
     2281 atcatcttcc tttaatttcc ctcttaaagc ataaatagac ggaatttgct tttctaatac
     2341 ttcccaattc ccgatatgca aatcgagaaa ttgaggcaag cgttccatat acatatcaaa
     2401 atagtatttt gggggattta atggagcatg tggtttttca aaagacattt tgagaaaaaa
     2461 aggaaccgtt ggatctcttc tttgtaaaaa tgaaatggac tcactcacaa cccagtttgt
     2521 tggatgtaac ttctcatctt tatcccaagg tctagcttcc caagaattac aatccattcc
     2581 gtcatcaatc aaatcaacat cataaccaac tttacccttt aaaaaggcta gatagtcact
     2641 agcataatca aattgacttc cataagcctt atcatacttt ctatcaacat gtagataccc
     2701 atcgtgaagt aagacatgat caaacccaag tctctgtctg gaaggaaaaa catgcatctt
     2761 accaatacac tctgtttgat aacccatatc tttaaagact tttggaagag tattagtaaa
     2821 attccaaggc acctcatctt gataaccaac tcgtccactt ttatcttggt ctaagccagt
     2881 caataaagct gccctagcag gaacgcaaga aggaactgga gaataggcat tttcaaaatt
     2941 ataacctaca ctagccatca tatctaatgt tggagtgcta actagcttat ccttactatt
     3001 caaggataag gcgtctgctc tcatttgatc tacaacaatc aaaataatat ttggctgttt
     3061 tgtctgaacc ataaaatctc ctttctaata tggcaaaaga ggcacaagaa gatatctacc
     3121 tttactgcac ccctttctat atcaat
//
LOCUS       DAVMEI010000163         3103 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVMEI010000163 DAVMEI010000000
VERSION     DAVMEI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.163"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..729
                     /gene="ccdA2"
                     /locus_tag="VYQ24_001794"
     CDS             22..729
                     /gene="ccdA2"
                     /locus_tag="VYQ24_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1968896.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLSVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            740..1297
                     /gene="sdbB"
                     /locus_tag="VYQ24_001795"
     CDS             740..1297
                     /gene="sdbB"
                     /locus_tag="VYQ24_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1968897.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKIVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            1671..3062
                     /locus_tag="VYQ24_001796"
     CDS             1671..3062
                     /locus_tag="VYQ24_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1968898.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT          847 a          541 c          650 g         1065 t
ORIGIN      
        1 attaagaaag ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca
       61 ggtgttttat cctttttttc tccttgtatt tttcctcttc tgtcagtcta tgctgggatt
      121 ttattggatg atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg
      181 tcaggcttga ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta
      241 ggctttggtg ctggttactt tggtcatatt ctctatgcaa attggtttcg atatggcatg
      301 ggagctatta ttatcatttt gggtcttcac cagatggaaa tttttcattt gaagaaatta
      361 gaagttcaaa aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt
      421 ttacttggta ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct
      481 gttttagcac ttgcggcttc tggaggcaat ggcgcttggc aaggagcgat ttatactctc
      541 atttacactc tgggcatggc ccttcctttc ttggtattgg cactagcttc aggtctagtc
      601 atgccatatt ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc
      661 ctcattgttt taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt
      721 tttgaataaa ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt
      781 agttgtattg atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc
      841 aaaacaacaa acgactgtac aacaaattgc tgttggaaaa gatgctccaa acttcacatt
      901 gcaatccatg gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt
      961 gaagttttgg gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact
     1021 agcggcgaaa ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg
     1081 tgaaaaaatt gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc
     1141 agttctttat gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac
     1201 agaatattta attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa
     1261 tgcggatgca gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag
     1321 atgtagtcag attttggtaa cttctaaaac taacttccag tttcccacaa cctagctttt
     1381 agtatgagaa aaacgcgtga catcagtgga aatccgtctt agactaactt ccactggttt
     1441 tctttttctt gatatataag ggttcaaaag cgaaaagact cagaaaaagt gcacgacaaa
     1501 tagccctaaa acagagtcgt cttagagtaa cttccagttt tgggagagag atggaagtta
     1561 ctttgagaag ttacggtagt cagatttttt tagaaaaaca ttttataaat attcacatat
     1621 ctcctatatt tatggtaaaa tagaatcatc agtttatttt ggagtcaaag atgaatatat
     1681 ttagaacaaa gaatgttagt ttagataaaa cagagatgca taggcatttg aagttatggg
     1741 atttgatttt gctgggtatc ggagccatgg tagggacagg cgtctttaca atcacaggta
     1801 ctgcagctgc aacacttgct ggcccagccc tagtgatttc aatcgttatt tctgccttgt
     1861 gtgtgggatt atcagccctc ttttttgcag aatttgcctc gcgagtaccc gctacaggag
     1921 gtgcctatag ttacctctat gctatcttag gagaattccc tgcctggttg gctggttggt
     1981 taaccatgat ggagttcatg acagccatat caggcgtagc ttcgggttgg gcagcttatt
     2041 ttaaaggctt gctctctcaa tacgggatag cctttcctca ggctttaaat ggtaccttta
     2101 atcctcaagc agggacattt gttgatttat tgcctattct tgtcttggtc ttggtgactt
     2161 cgcttgtttt actaaatgcc aaggcagcct tacgctttaa ttcgattcta gttattttga
     2221 aattttccgc tttagctctc tttgttttag taggaatttg gaatattaag tttgataatt
     2281 ggagcaattt tgctccttac ggttttggac aaatctatgg agctagcact ggtattatgg
     2341 ctggtgcgtc cttgatgttc ttcggttttt tgggatttga atccatctct atggctgtag
     2401 atgaagtcaa gactcctcaa aaaaatattc ctagagggat tgtcttatcg ctttctatcg
     2461 taaccattct ctatgccttg gtgacccttg ttttgactgg tgtggttcac tatagtcatt
     2521 taaatgtcga tgatgccgtt gcctttgccc ttcgtagtgt tgggattagt tgggcagcca
     2581 actatgtgtc attagtggct atcttgacct tgattacagt ttgtatttcg atgacctatg
     2641 ccctatcgcg tatgatttac agtttagcac gtgacggctt agtgcctgct gcctttaagg
     2701 aactgacgaa gactagcaag ataccaaaga atgctactat cttgacaggt ctagcttcag
     2761 cagtagcagc aggaatgttc ccactagcca gtattgcagc ctttttaaat atttgtacct
     2821 tagcctactt gatcatgttg gcttatggtc tgattcgctt acggaaagaa aaaggaatgc
     2881 ccaaagctgg ggaatttaaa acaccattgg tacctctgtt accaatttta tcgatcatca
     2941 tttgtttatc cttcatgtta cagtataata tgaatacctg gattgctttc ctagttgcgt
     3001 tgttagtagg aagtattatt tacttcactt atggctataa gcattctacc atagaagaat
     3061 aaagtgaaag tctattgagt tgttgacact cagcagattt tta
//
LOCUS       DAVMEI010000164         3095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVMEI010000164 DAVMEI010000000
VERSION     DAVMEI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.164"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(69..273)
                     /locus_tag="VYQ24_001797"
                     /pseudo
     CDS             complement(69..273)
                     /locus_tag="VYQ24_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(541..780)
                     /locus_tag="VYQ24_001798"
     CDS             complement(541..780)
                     /locus_tag="VYQ24_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1968899.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(800..1072)
                     /gene="rpsP"
                     /locus_tag="VYQ24_001799"
     CDS             complement(800..1072)
                     /gene="rpsP"
                     /locus_tag="VYQ24_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1968900.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            1242..1451
                     /locus_tag="VYQ24_001800"
     CDS             1242..1451
                     /locus_tag="VYQ24_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968901.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            1930..2151
                     /locus_tag="VYQ24_001801"
     CDS             1930..2151
                     /locus_tag="VYQ24_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968902.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            2235..3038
                     /locus_tag="VYQ24_001802"
     CDS             2235..3038
                     /locus_tag="VYQ24_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1968903.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
BASE COUNT          954 a          622 c          546 g          973 t
ORIGIN      
        1 aaaatcctta tcttttattt tatcacgttc caaggatttt ctcaagttgg aggaagggga
       61 caacatctct actttccctt caaaaatcct tccaaatcat gtttatgttg gtaattaacg
      121 gctgcggttt tgtctttctc aaagacagtc ttggtaaggt caatatgatt aatagctacg
      181 attgcgacgg tgtagtaaat gatatcagcc agttctctgg caagttcctc gttcgaatcc
      241 tatcccttct tttcgaccag agcgcctatt caaaacctcg gctacttctc cgacttctcc
      301 actaacttca taaagagacc ttcatcagtc cgagactgct gttaatgttc gagtaagtag
      361 tcttggaatt gcctaaacgt tcaatctttt atagtatatt gaaactagaa tagtacacct
      421 ttacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttg tcctgttatt
      481 atttcatttt actatatctt ctattccaca caaaaaagcg agacatccgt cccgcccttc
      541 ttatttttcg tcaataacga ttcttacttt tttgtattca gttgggacag agtagacaat
      601 cgttcttatc gcagaaatag tgcgaccctt acgaccgatt acacgaccca catcgctttg
      661 atcaagattc aaatgatatt ccaaaaattc tggtgtatcc tcaatcttga tagttaaggc
      721 atctggttgt gaaattaagg gtttcacaat cgcaataatg agattttcaa tcgtatccat
      781 ctgtcaacct actttaaact tattttgaaa atttagaatc gtggaatttt ttcaatacgc
      841 cttcttttga aaggatgtta cgtactgtgt ctgaaggttg agctccatta gccaaccatg
      901 caagaacgcg gtcttctttc aaagttactt ggttttcagc aacaagtggg ttgtaagttc
      961 caactgtttc gatgaaacgt ccgtcacgtg gtgaacgtga atctgctacg ttgatacggt
     1021 agaaaggttt tttcttagaa cccatacgag tcaaacggat tttaactgcc atttttaaag
     1081 tctcatttct ttaatttttt atttcggtga aatagctgag ctatctagca catgttctat
     1141 tatagcagat ttctggcatg tgtcaagaaa aaaacttgac agactataaa tttcttaaag
     1201 tatttagaaa tttgttagaa taggaaataa atgtttgaaa gatgctatcc gtgaatacta
     1261 ttttagaaaa attttataaa gaacaccaag tcaaaccctt catatcgcct gaacgagagt
     1321 tggatacttg gcttctaagt cccaagcccg ttcccaagcg aaatatggac ttattagtag
     1381 atgattcact agcaggagat atcattttac tatggagaat ccagtttggt acttttacca
     1441 ctgaaacatg attttaaaaa ctcccattcg aatatgattc aaaattcgct tagaatcaat
     1501 gttttcccac tttttcaaaa aataaatttt catctaattt aaataacttt caattaagtg
     1561 tgaattttgc ttaaaatcat taaaaaacac cccatggtgt gaaccatgaa gtgttgtaaa
     1621 tgctgtgttg tagctagttc ttagcgttta gagaactggc tagctttacg agctttctta
     1681 agatctggtt tccaaaatac tttgagtaca cttatggtat tgatgcccct aaacatctaa
     1741 aaactctggt agaaaaaggg tatgctcttg tcgaaacttc ttttgactca cttgaccatc
     1801 taaatactac catgaaaaag aatatcctga agaagaaggg agttactgga ctttctaaaa
     1861 tgaaatcttg cgacctggat caagcccttc atgagcattt ttcagaagaa gaattagctg
     1921 gtcactttca tgtccttcta tggacttttt tacaatggca ttgctatcac acccaatacc
     1981 tatctaagcg cctggttcgt aaactttatt gcagctcttc ctctaaattt cctaattgtt
     2041 gaaccaattg cccgttttat actaagttct tttcagaaac catttactgg ggaagaagtt
     2101 gaagaagttg aagattttca agatgatgat gaaatcccaa ctattatcta agccagttct
     2161 gtaaactact aatatttgaa atccacttcc ttttagggta caatggttat aaatgaattt
     2221 ttgagaggat cagaatgaaa aaactagcaa cccttctttt actgtctact gtagccctag
     2281 ctgggtgtag cagcgtccaa cgcagtctgc gtggtgatga ttatgttgat tccagtcttg
     2341 ctgctgaaga aagttccaaa gtagctgccc aatctgccaa ggagttaaac gatgctttaa
     2401 caaacgaaaa cgccaatttc ccacaactat ctaaggaagt tgctgaagat gaagccgaag
     2461 tgattctcca cacaagccaa ggtgatattc gcattaaact cttccctaaa ctcgctcctc
     2521 tagcggttga aaatttcctc actcacgcca aagaaggcta ctataacggt attaccttcc
     2581 accgtgtcat cgatggcttt atggtccaaa ctggagatcc aaaaggggac ggtacaggtg
     2641 gtcagtccat atggcatgac aaggataaga ctaaagacaa aggaactggt ttcaagaacg
     2701 agattactcc ttatttgtat aacatccgtg gtgctcttgc tatggctaat actggtcaac
     2761 caaacaccaa tggcagccag ttcttcatca accaaaactc tacagatacc tcttctaaac
     2821 tccctacaag caagtatcca cagaaaatta tcgaagccta caaagaaggt ggaaacccta
     2881 gtctagatgg caaacaccca gtctttggtc aagtgattga cggtatggat gttgtggata
     2941 agattgctaa agccgaaaaa gatgaaaaag acaagccaac tactgctatc acaatcgaca
     3001 gcatcgaagt ggtgaaagac tacgatttta aatcttaaaa accaaaaaaa tacagtatcc
     3061 acattcggta ctgtatttct ggctctataa tatct
//
LOCUS       DAVMEI010000165         3093 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVMEI010000165 DAVMEI010000000
VERSION     DAVMEI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.165"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..267)
                     /locus_tag="VYQ24_001803"
     CDS             complement(<1..267)
                     /locus_tag="VYQ24_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968904.1"
                     /translation="MIGKNIKSLRKTHDLTQDDFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANL"
     gene            complement(338..610)
                     /locus_tag="VYQ24_001804"
     CDS             complement(338..610)
                     /locus_tag="VYQ24_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968905.1"
                     /translation="MFTKLFKKNQDNSDIFKKLIHRLSDMSVQDLEKIDRLLDIIFTP
                     DQGSEQLKTESTYREETLDDTLKEAKNQLHKEQLEKNLERFRKNSQ"
     gene            complement(648..1031)
                     /locus_tag="VYQ24_001805"
     CDS             complement(648..1031)
                     /locus_tag="VYQ24_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEV1968906.1"
                     /translation="MTKDWNFNQPLESKSENQEDPDKIAALFGNHQGGNDVNYEAAFQ
                     KRKQAPVTESNSSSKPKVTEVRTGKETDITTSYQQHLKRLIADNNSDIQSSQKKIEEL
                     HTLIDTKNKDNKKLQSIYDAISELH"
     gene            complement(1028..1261)
                     /locus_tag="VYQ24_001806"
     CDS             complement(1028..1261)
                     /locus_tag="VYQ24_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968907.1"
                     /translation="MTIIERLEEKVTRQENKVARETEKLAAYKEQLETAMFATFKRRQ
                     SISHMSFEEALDHAFGRERQFDDSEFRKDEMSE"
     gene            complement(1312..2397)
                     /locus_tag="VYQ24_001807"
     CDS             complement(1312..2397)
                     /locus_tag="VYQ24_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEV1968908.1"
                     /translation="MEIEECKQISILDVASRLGISFKQVSSSIYELPEHDSFRIFSNT
                     NTFKWFSRDIQGDVIDFVRLVKGISFKEALGFLSQEPFQKEAVQEKRERPFYYPLKRI
                     EDSNCSLARYYLTECRGISEEIIQKMIQQGLIAQASWKTNETVEPVIVFKSFNHRHKL
                     QAASLQGIYKNPALSRERLKTILKGSHGNVGISFDIGKPNRLVFCESFIDLMSYYELH
                     QQSLSDVRLVSMEGLKRSVVAYQTLRLIAEENQKLEFLDTVTPSKLLPLINTIRDTTT
                     YFNNHPDLLTLAVDCDDAGKDFSDKLSQEGFPVLLDLPNNEFGKEKIDWNDILREKKS
                     DLQLMIETAKETLRNQPVRQTSQCLEL"
     gene            complement(2407..3045)
                     /locus_tag="VYQ24_001808"
     CDS             complement(2407..3045)
                     /locus_tag="VYQ24_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1968909.1"
                     /translation="MLNKVKTKALISVGAVATTSFILMMGYTVGQHSTAKQSRKEIEL
                     AAAKLVEDKQAEDKASILSSDTVKEFLTQYYTKEKLGENNTRIQPYMTESAYSQELTS
                     QNDAMNQVYKDYILDYHFEKADIFVNQTTNQAIAMVSYNVTYVSDLKNANQSKTNQTE
                     TRTVQLSYSKLPGKLLVNQVQVWKSGLDDLDKATTKTLEESSIPSLPNTTTK"
BASE COUNT          881 a          581 c          464 g         1167 t
ORIGIN      
        1 tagattagca cctctttctt tcacaatttc aatttttaaa gtcaattcat aatcttcaac
       61 aggattaagc attttatctt ctcctacaat atcgacataa gatacattaa acttctgaca
      121 aatgatgtct attagttccg tagagactga actcgttcca ttttcatacc ggctcaagct
      181 atttcgagaa attcctacaa ttcgggcaaa gtcgtcttgt gttaagtcat gtgttttacg
      241 taaggatttt atgttctttc caatcatggc aaactccttt tatttttata ccaccattat
      301 aacatttaga aaaagtaaat gcaccatttt tggtgcatta ttggctattt ttcctaaatc
      361 tctctaaatt cttttccaat tgttccttat gaagttgatt ctttgcttcc tttaatgtgt
      421 cgtccaaagt ttcttctcta taagttgatt ctgtttttag ttgttctgat ccttgatctg
      481 gagtgaaaat gatatctagc agtcggtcta ttttttcaag atcttgaacg gacatatcag
      541 atagtcgatg aattagcttc ttaaatatat cactattgtc ttgattcttc ttaaaaagtt
      601 ttgtaaacat ctatagcctc tcaaaaaaag cagcctctta ggactgctta atgtaattct
      661 gaaatcgcat cataaattga ctgcaatttc ttattatctt tattctttgt gtcaattaac
      721 gtatgtagct cttcaatttt cttctgactg ctttgaatat cgctattgtt atccgcaatc
      781 agtcttttaa gatgttgctg ataacttgta gtgatatctg tttcttttcc tgttctaacc
      841 tctgtaactt tgggtttaga actagaattt gattctgtca caggtgcttg ttttctcttt
      901 tgaaaagctg cttcataatt tacatcatta cctccttgat gatttccaaa taaggccgca
      961 attttatctg gatcttcttg attttctgat ttactttcta atggttgatt aaaattccaa
     1021 tcttttgtca ttcactcatt tcatcctttc taaattcaga atcatcgaat tgtctttctc
     1081 taccgaaggc atggtcaaga gcttcttcaa aactcatgtg actaatgctt tgacgccttt
     1141 tgaacgtcgc aaacatcgct gtctccagtt gctctttgta agcagcaagt ttttctgtct
     1201 ctcttgctac cttgttttct tgcctagtga ccttttcttc taggcgttca ataatggtca
     1261 tatacgaacc tccttcattt taatattagc taaaacatct tgattttgtt atcacaattc
     1321 taaacattga gaggtttgtc ttactggttg attcctcaat gtctcttttg cagtctcgat
     1381 catcaattgt aaatctgatt tcttttctcg aagaatatcg ttccagtcta ttttttcttt
     1441 cccaaattca ttattaggta aatccagtaa aacaggaaat ccttcttgag ataacttatc
     1501 agaaaaatct tttcctgcat catcacaatc taccgcaagt gtcaataaat caggatgatt
     1561 attgaaatag gtagtggtat cacgaattgt attgatcaaa ggcaataact ttgaaggtgt
     1621 tactgtatct aagaattcca acttctgatt ttcttcagct atcagtcgta aagtttgata
     1681 agcaacaaca gaccttttta atccctccat agataccaag cgaacatctg atagactttg
     1741 ttgatgaagt tcgtaatagc tcatcaagtc gataaacgat tcacaaaaga ccaatctatt
     1801 tggtttacca atgtcaaagg atattccaac atttccatgg cttcctttta gaatcgtttt
     1861 taacctctct ctagaaagag cgggattctt ataaatccct tgtaagcttg ctgcctgcag
     1921 cttgtgacga tgattaaagc ttttaaaaac gataacaggt tcaactgttt catttgtttt
     1981 ccagctggct tgtgctatta aaccttgttg aatcatcttt tgtatgattt cttctgagat
     2041 tcctctacat tctgttaagt aatatctggc cagactacag tttgaatctt ctattctctt
     2101 taaaggataa taaaatggtc tctctctctt ttcttgaaca gcttcttttt gaaaaggttc
     2161 ttgagaaaga aaacctaaag cttctttaaa agagattccc ttaacaagtc gaacaaaatc
     2221 aatgacatca ccttgaatat ctcttgaaaa ccatttaaaa gtattggtat tcgaaaaaat
     2281 ccgaaatgaa tcgtgttcag gaagctcata tatactgcta gaaacttgtt taaaggagat
     2341 acctaaacga cttgctacat caagaattga aatttgctta cattcttcta tttccatgca
     2401 atatcatcat tttgtcgtag tatttggcag tgatggtatt gatgattcct ctaaagtttt
     2461 agtagttgcc ttatctaaat catctaaccc agatttccaa acctgcactt ggttgaccaa
     2521 taacttacca ggaagtttag aataagataa ttgaactgtt ctggtttctg tctgattggt
     2581 ctttgattgg ttggcattct ttaaatcaga tacataggtt acattataag acaccatggc
     2641 aatggcttga ttcgtagtct gattgacaaa aatatcagct ttttcaaaat gataatccaa
     2701 aatatagtcc ttataaactt ggttcatagc atcattttga cttgtcaatt cttgagaata
     2761 agcagattca gtcatatagg gctgaatgcg tgtattattt tctccaagct tttctttcgt
     2821 ataatactgt gtcaaaaatt cttttacagt atctgatgac aaaatgcttg ctttatcttc
     2881 tgcttgtttg tcctctacaa gtttagctgc agccaattcg atttctttgc gactttgttt
     2941 agcagtagaa tgttgcccaa cagtatatcc catcatgaga ataaagcttg ttgtggctac
     3001 tgctccaaca ctaattaagg ctttagtttt gactttattt aacatcttag accttctttc
     3061 tataaaaact aggtcaagaa taacaaaaag aac
//
LOCUS       DAVMEI010000166         3061 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVMEI010000166 DAVMEI010000000
VERSION     DAVMEI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.166"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            415..2520
                     /locus_tag="VYQ24_001809"
     CDS             415..2520
                     /locus_tag="VYQ24_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1968910.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQNKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
     gene            2971..3061
                     /locus_tag="VYQ24_001810"
                     /pseudo
     CDS             2971..3061
                     /locus_tag="VYQ24_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          977 a          548 c          715 g          821 t
ORIGIN      
        1 taatatatag ttttgagatg gttgatagaa ctgacgaagt cagctcaaaa catggttttg
       61 aggttgtaga taaggagaag ctgatgtggt ttgaagaggt tttcgaagag tgtaaaaaaa
      121 tattggtcag ttaaagccaa aactattgac cttttctgac caatgtagta tattatgaac
      181 gtaaggtaaa tgaaagcctt actagaacac ttatttaaag taaaatagaa agaggtgggt
      241 ctatgtttca tctagaaatc ttcagaagta aagatggtct actcctgctt gaaaaagaaa
      301 aaccagaaat agtacgtagg gtagcggttt aactagtcta aattttttaa aacaatggtc
      361 aaagatagtc aatatcagta atcataacta agtaaacaaa aagaggtaaa gaatatgaat
      421 aacaacttta ataattttaa taacatggat gatttattta accaattgat gggtggtatg
      481 cgaggatata gttctgaaaa tcgccgttac ttaattaatg gacgcgaagt cacacctgag
      541 gaatttgctc actatcgtgc gactggtcaa ttaccaggaa atgcagaaac tgatgtgcaa
      601 atgccacaac aggcatcagg tatgaaacaa gacggtgtcc ttgcaaaact aggtcgaaac
      661 ttgacagcag aagcgcgtga gggcaagttg gatcctgtta tcggacgaaa caaggaaatt
      721 caagaaacat ctgaaatcct ctcacgccgc accaagaaca atcctgtttt ggtcggagat
      781 gcaggtgttg gtaagacagc agttgtcgaa ggtctagcgc aagccattgt gaacggagat
      841 gttcctgctg ctatcaagaa caaggaaatt atttctattg atatctcagg tcttgaggct
      901 ggtactcaat accgtggtag ctttgaagaa aatgtccaaa acttagtcaa tgaagtgaaa
      961 gaagcaggga atattatcct cttctttgat gaaattcacc aaattcttgg tgctggtagc
     1021 actggtggag acagtggttc taaagggctt gcggatattc tcaagccagc tctctctcgt
     1081 ggagaattga cagtgattgg ggcaacaact caagacgaat accgtaacac catcttgaag
     1141 aatgctgctc ttgctcgtcg tttcaacgaa gtgaaggtca atgctccttc agcagagaat
     1201 acttttaaaa ttcttcaagg cattcgtgac ctctatcaac aacaccacaa tgtcatcttg
     1261 ccagacgaag tcttgaaagc agcggtggat tattctgttc aatacattcc tcaacgtagc
     1321 ttgccagata aggctattga ccttgtcgat gtaacggctg ctcacttggc ggctcaacat
     1381 ccagtaacag atgtgcatgc tgttgaacga gaaatcgaaa cggaaaaaga caagcaagaa
     1441 aaagcagttg aagcagaaga ttttgaagca gctctaaact ataaaacacg cattgcagaa
     1501 ttggaaagga aaatcgaaaa ccacacagaa gatatgaaag tgactgcaag tgtcaacgat
     1561 gtggctgaat ctgtggaacg aatgacaggt atcccagtat cgcaaatggg agcttcagat
     1621 atcgaacgtt tgaaagatat ggctcatcgc ttgcaaaaca aggtgattgg tcaagataag
     1681 gccgtagaag ctgtagctcg tgctatccgt cgtaaccgtg ctggttttga tgaaggaaat
     1741 cgcccaatcg gcagcttcct ctttgtaggg tctactgggg ttggtaagac ggagcttgct
     1801 aagcaattgg cactcgatat gtttggaacc caggatgcga ttatccgttt ggatatgtct
     1861 gaatacagtg accgcacagc tgtttctaag ctaattggta caacagcagg ctatgtgggt
     1921 tatgatgaca atagcaatac cttaacagaa cgtgttcgtc gcaatccata ctctatcatt
     1981 ctcttggatg aaattgaaaa ggctgatcct caagttatta cccttctcct ccaagttcta
     2041 gatgatggtc gtttgacaga tggtcaagga aatacagtaa acttcaagaa cactgtcatt
     2101 attgcgacct caaatgctgg atttggctat gaagccaact tgacagaaga tgcggataaa
     2161 ccagaattga tggaccgttt gaaacccttc ttccgtccag aattcctcaa ccgctttaat
     2221 gcagtcatcg agttctcaca attgactaag gaagaccttt ctaagattgt agatttgatg
     2281 ttggctgaag ttaaccaaac cttggctaag aaagacattg acttggtagt cagtcaagcg
     2341 gctaaagatt atatcacaga agaaggttac gacgaagtca tgggggttcg tcctctccgt
     2401 cgcgtggttg aacaagaaat tcgtgataag gtgacagact tccacttgga tcatttagat
     2461 gctaaacatc tggaagcaga tatgaaagat ggcgttttgg ttattcgtga gaaagcctaa
     2521 gacagaattt tgaggataaa aaagaaggag ccagctgaaa aaactggttc cttttgaggt
     2581 acgacgggca tgtcgtacag taggttgaaa ctagaatagt acacatctac ttctaaaata
     2641 ttgttagaaa tcgatttgac tgtcctgatt gatttgtcct gttcttattt cattttacta
     2701 tatatctgag gtgtaagtcc tcggtgggta cctgctaccg gtgctcccaa tagtgattcc
     2761 caagcctgac tatcgtgagg tagcggatta aaacggtctg gggatagacc gttttaagtc
     2821 tgacgctgga aataagaatc gtcagaggaa gggatagcga aatcgtggct ctacgaatag
     2881 gaacgtgata ataaggcgta tatagcggat aaggggcctc aaactctaaa gttcaaaaag
     2941 gtagtcgtag cctatatgtg taaatcacga gagcaactga atttgtctgt tcgaggatgg
     3001 ataaactatt tctcattggg aaatatgaaa agtatagtca ctagcataga tgagcgcttg
     3061 c
//
LOCUS       DAVMEI010000167         3050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVMEI010000167 DAVMEI010000000
VERSION     DAVMEI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.167"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(233..766)
                     /locus_tag="VYQ24_001811"
     CDS             complement(233..766)
                     /locus_tag="VYQ24_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1968911.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENTIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(855..1631)
                     /gene="recX"
                     /locus_tag="VYQ24_001812"
     CDS             complement(855..1631)
                     /gene="recX"
                     /locus_tag="VYQ24_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1968912.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1669..3024
                     /gene="rlmD"
                     /locus_tag="VYQ24_001813"
     CDS             1669..3024
                     /gene="rlmD"
                     /locus_tag="VYQ24_001813"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1968913.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT          972 a          535 c          552 g          991 t
ORIGIN      
        1 tattttttgt attaaacgat ataagtttgt tgtaaactta ctaggaataa agacattaaa
       61 aaataatagt atatctattt gttttatata ttttacgaat tctgcataaa tctctttcta
      121 gtaatgtgtt gtaactctgc tataatggat ttattccttt ttgtatttac acaatttatt
      181 ttatactacc aaaaaaacaa ggtcaggatt ttgttcctga cctttgacaa ctttaccgat
      241 tctttagttc tacatagcgc ttgtaccaaa tgtttacata ggcttctgag aaaggaccac
      301 gtccattgtt aatccaatca acaagaattt tgacatgttc ttttaaaata tagtccaagt
      361 catcagaata attcattttg cgtttgtgac gctcgtattc ttcaacgtcc aagagacgtt
      421 tttccccatc tgtaaaaatt ttaacatcca aatcgtaatc aatatacttc agtgcttctt
      481 catccagata gtaggggcta gccatattgc aatagtaaga agttccatta tcacgaatca
      541 tggcaatgat attaaaccaa tatttcttgt gaaagtaaac aatagccggt tctcgagtga
      601 cccaacgacg accatcactt tcggtaacaa gtgtatgatc gttgacacca ataatggtgt
      661 tttctgttgt ttttagtacc atggtgtccc gccaagttcg gtggagactc ccatcatgct
      721 tataactttg aattgtaata aagtcgcctt cttttggaag cttcataact aaccaacttt
      781 ctacaattta taagtttatc atttactatt gtaccataaa attacctaaa atctgtgaat
      841 ttcacttgga aatattaaag atattctcta agagcgcttg ctatatccga aaaatcgtag
      901 ccctttcgtg ctaaaacttg agttaaacgc tgcttcagtt cgtatccttc atactttcga
      961 gcatacttag tatattgctt atcaagttcc ttgaagatga gttcttgagt cgtttcttgg
     1021 tcaacttggc tatctaagtc gtcaaaggca attttagcat cagaataaga gaagcccttg
     1081 ttagtcaagt tctggataat cttatcttgc agggcacgag ctggaagttt tccctcatat
     1141 tttttcaata gtttattggc tacacgttga gcaacttcag aaaaatcaaa ttctttcaag
     1201 ttctcttcta tagtagattt tgaaatccct ttttgagata gtttctgagt cagcacataa
     1261 ggacccttgt ctcctgaaag ttgattggta ttgatgatag cataagcgta ctgaccatca
     1321 ttaatccact tatcttcttt aagattagca ataacttgag aaacgatgtt tttatcaata
     1381 tcgtattttt tcagatattc tctgacttct ttttcagtgc gtgctttaaa ggataagtgg
     1441 tagagagcga gattcttacc ataagaaaat tgagcaaagt cttgaatctc tttcaattcc
     1501 tcttcgctta tcaccttatc tctcgataac ataaaacgaa caattgtatc ttcggtgata
     1561 tagcatttgt cgccattatc aagctccatc aggtagagtc tttttttctt ttcaagtttt
     1621 gtgattttca tagctctatt ataactcaaa atgtgataag ataggggtat gaatctgaaa
     1681 gtgaaacaaa aaataccatt aaaaatcaag cgcatgggaa ttaacggtga gggaatcggc
     1741 ttttaccaaa aaacattagt ctttgtacca ggagctctca aaggcgaaga tatctattgt
     1801 cagattactt ctattagacg caactttgtt gaagcaaaat tactgaaggt caacaagaag
     1861 tctaaatttc gaattgtgcc atcttgtact atttataatg aatgcggagg ctgccaaatc
     1921 atgcacctgc attatgataa gcagctggag ttcaagacgg acttacttca tcaagcgctg
     1981 aaaaaatttg ctcctgcagg atatgaaaat tatgaaattc gtccaactat tggaatgcag
     2041 gaaccaaaat attacagagc taagttacaa tttcagactc gaaaatttaa aaatcaggtc
     2101 aaggcgggct tatatgcaca aaactctcac tatttagtag agttgaaaga ctgcctggta
     2161 caagataagg aaacccaagt gattgctaat cgcttagcag aattacttac ttatcaccag
     2221 attccaatca cggatgagag aaaagttcta ggtgtccgta ctattatggt ccgacgcgcg
     2281 agaaagaccg gacaggttca gattattatt gttacaaacc gccagcttaa tttaactcaa
     2341 ttggtaaaag agttggttaa agatttccca gaagttgtga cagtagctgt taatacaaat
     2401 acagctaaaa ccagtgagat atatggtgaa aagacagaga ttatctgggg gcaagagagt
     2461 attcaagaag gtgtactcaa ttatgaattt tcactatccc ctcgagcttt ttatcaacta
     2521 aatcctgagc aaacagaagt cctctatagc gaagcagtaa aagcgctgga tgttgataaa
     2581 gaagaccatt tgattgacgc ttattgtgga gttggaacga ttggatttgc ctttgcaaag
     2641 aaagttaaaa cactcagagg tatggatatt attccagaag ctattgaaga tgccaagcga
     2701 aatgctaaaa gaatgggatt tgacaatact cattatgaag ctggaacggc agaagagatt
     2761 attcctcgtt ggtacaagga aggctaccga gcagatgctt tgattgttga cccaccacgt
     2821 acaggtctgg atgataagtt attagatact attcttactt atgtaccaga aaaaatggtt
     2881 tatatttctt gtaatgtttc gaccttggct cgtgatttgg tacgcttagt agaagtctat
     2941 gatcttcatt atatccagtc ggtcgatatg ttcccacata cagctcgaac tgaagctgtt
     3001 gtaaaattaa taaaaaaagt ttaaaaaagt agttgacaaa gtttgaaaag
//
LOCUS       DAVMEI010000168         3047 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVMEI010000168 DAVMEI010000000
VERSION     DAVMEI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.168"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="VYQ24_001814"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="VYQ24_001814"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1968914.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="VYQ24_001815"
     CDS             complement(1169..1693)
                     /locus_tag="VYQ24_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1968915.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /locus_tag="VYQ24_001816"
     CDS             complement(1746..1982)
                     /locus_tag="VYQ24_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1968916.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /locus_tag="VYQ24_001817"
     CDS             complement(2050..2970)
                     /locus_tag="VYQ24_001817"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1968917.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
BASE COUNT          880 a          557 c          627 g          983 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaccccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttgatgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 tgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa aaaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga tacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttggaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagttctctc tgatttcgaa gaatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgcgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tactttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgcagatttg tgagattgat
     2161 actggtccag acctgatggg caaaggactc cacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc taggctcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttaattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctt ttcgggtacg atgtcgtaaa cttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctagatattt tgtctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt ggtaagt
//
LOCUS       DAVMEI010000169         3006 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVMEI010000169 DAVMEI010000000
VERSION     DAVMEI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.169"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(85..1026)
                     /locus_tag="VYQ24_001818"
     CDS             complement(85..1026)
                     /locus_tag="VYQ24_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1968918.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VYQ24_001819"
     CDS             complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VYQ24_001819"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1968919.1"
                     /translation="MKRSMYAGRVREEHIGQEIALKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          874 a          708 c          598 g          826 t
ORIGIN      
        1 agatagaatt gacgaagtca gtaacatata tacggcaagg cgacgctgac gtggtttgaa
       61 gagattttcg aagagtatta atcactaatt atctatctca acaaatcttc ctagaatatg
      121 aacattttcc gagacagaga caaaggagct tggatccact tgtgtcataa tctgtttaaa
      181 ttcattaaac tctgcacgtg taatgacagt gattaaaact gcctttctct cgtgattata
      241 ggttccttct gcatcgtgga tcatggttgc tccgcggtgc aattttttat ggattttttc
      301 aattaccttc tctggatgat ttgtcacaat catggcctgc atacgctttt gcttagtaaa
      361 gactgcgtct gtcacacggc tagagacaaa gatggtaatc atagaataaa gagcgtattt
      421 ccaaccaaag gtcaaacctg ctatcagcat gatagttcca tttaccaaga aagaaatact
      481 accgacattc ttacccgttt tcttacgaat agtcaggctg acgatgtccg tcccaccact
      541 ggagatattg tttcgaagag caaaaccaat ccccaaaccc ataacaacac ccccaaaaag
      601 ggcattgata atgggatcct ctgtcaaggt tgccacaggg acaaactgga taaagaagga
      661 actcatagat accgtgataa aggtaaagac ggtgaactta tggccaatct gataccaagc
      721 taagaccatc aaagggaagt taatggcgta aaagcttagc gaaatcggaa tatgaaaacc
      781 aaaccagtga ttactcaagg cagagataat ctgtgccaga cctgttgcac cactcgaata
      841 cacatgccct ggttggaaaa agaaattaac tgctactgct gataaaaaac catagaccag
      901 agaggccgaa atcttctcat catacttttc tcgagagata ctttgtaaga cacgtaaaat
      961 ttttatctga taagcaaagc ggcgcagata atagcgccac cgcttaattc gttttgtttg
     1021 tttcatcttc ttctacttgt aagctgagtt cctctagttg tttgagagcg actgttgatg
     1081 gagcttgtgt cattgggtca gttgccttgt tgttcttagg aaaggcaatg acttcacgga
     1141 tattttcttc tccagcaagc aacatgacaa aacggtcaag cccgatagcc aaaccaccgt
     1201 gtggtgggaa accatagtcc atggcttcaa gaaggaaacc aaactggtca ttggcttctt
     1261 cagttgagaa accaagagcc ttgaacatgc gttcttgaag gtctttttgg ttgatacgaa
     1321 ggctaccacc accaagctca taaccgttca agacgatatc gtaagcaatg gcacgaacct
     1381 tagccaaatc accttctaat tcgtgagcag tctcttcctg tggaagtgtg aaaggatggt
     1441 gggcgctcat gtagcggcct tcttcttcag accattcaaa catcggccag tcaaccaccc
     1501 aaaggaagtt gaacttatca ttatcaatca agccaagctc tttagcaata cgtccacgaa
     1561 gggcacccag tgttgcatta gccacttcaa gcgtatccgc cacaaagaga accaagtcct
     1621 tatcttcaag aacaagcgct gttgtcaatt cttcttggat accagtcaag aacttggcaa
     1681 ctggtccgtt taattctcca tcaaccacct tgacccaagc aagacctttg gcaccatact
     1741 gtttggctac ttccgtcatc ttgtcgatgt ctttacgtga atagttgtcc gcagctcctt
     1801 tgaccacaat cgcttttaca gcaggtgctt ctgaaaagac tttaaagtct acacctttga
     1861 ccacttctgt caagtcctga agcaacatgt caaaacgagt atctggcttg tcagaaccgt
     1921 aaagagccat agcatcatcg tatttcatac gagggaacgg tagcgttact tcgatgcctt
     1981 ttgtttcctt catcacgcgc gcgatcaagc cttctgtaat atcttggatt tctttctcag
     2041 taaggaagga cgtttccaag tcgacctgag taaattcagg ctggcggtct ccacgcaagt
     2101 cctcgtcacg gaaacattta acgatttggt agtaacggtc aaaaccagca ttcatcaaga
     2161 gctgtttcgt gatttgtgga ctttgaggaa gagcgtaaaa atgcccctta ttaacacgag
     2221 acggcactaa ataatcacgc gccccttcag gcgttgactt agaaaggaat ggtgtctcca
     2281 cgtcgataaa ctccaactca tccaaatagt tgcggataga gtgggtcacc ttggcacgaa
     2341 gtttaagatt ttccaacatt tctggacgac gaaggtcaag gtaacggtaa cgcaaacgtg
     2401 tatcgtcatt tgcctcaatg ccatccttaa tctcaaatgg tgttgtctta gctgtgttaa
     2461 gcacaataag agctgtcacg tttaactcaa ccgcaccagt tggcaactta tcattggctt
     2521 gctcacgcgc agcgacctga ccagtcacct caataacaaa ttcgctacga aggctttcag
     2581 ctgttgccat aacctctgca gatacttttt cagggttgat aaccaactgc atgattcctt
     2641 cacggtcacg aagatcgata aagatcaaac caccaaggtc acgacgacgg ccaacccatc
     2701 ctttcaaggc tatttcttgt ccgatgtgtt cctcacgaac acgaccagca tacatactac
     2761 gtttcattat ttctctcctc ttttattctg ttactatttt accataaaag cgcaggctct
     2821 tcatgaaaat catcagaaaa gtttgccagt ctttaaaagt cagatgaaag ccctaaaaat
     2881 tagcgctaat actcttcgaa aatctcttca aaccacgtca gcgtcgcctt accgtaggta
     2941 tggttactga cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg
     3001 tcagtt
//
LOCUS       DAVMEI010000170         2994 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVMEI010000170 DAVMEI010000000
VERSION     DAVMEI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.170"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            47..796
                     /locus_tag="VYQ24_001820"
     CDS             47..796
                     /locus_tag="VYQ24_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1968920.1"
                     /translation="MFILIINRPLNDSQSFKTKSNIAQIEAQALKHLDKPIIDLSGWQ
                     RPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKTYKSHITELQKRNVPVA
                     VYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMNEGVENFRAKLASL
                     GAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKTDLDYDIHQYTSKG
                     KIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            891..1733
                     /locus_tag="VYQ24_001821"
     CDS             891..1733
                     /locus_tag="VYQ24_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1968921.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            1733..2215
                     /locus_tag="VYQ24_001822"
     CDS             1733..2215
                     /locus_tag="VYQ24_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1968922.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            2215..2835
                     /locus_tag="VYQ24_001823"
     CDS             2215..2835
                     /locus_tag="VYQ24_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1968923.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
BASE COUNT          905 a          617 c          558 g          914 t
ORIGIN      
        1 aaaacaaatt catccactta ttttatttag tttttttggg attatgatgt tcattttaat
       61 catcaatcgc ccacttaatg acagccaatc gtttaaaaca aaatccaata tagcacagat
      121 tgaagcacag gctttgaaac acttagacaa accgattatt gacctctctg gctggcagcg
      181 tcctgaggaa atcaactacg atgccctctc acaaaatatt tcaggtgcta ttgttcgtgt
      241 tcacagtggt gctcaaacaa caaaagaaaa tgatgcttca tttatcaatg gaatagacaa
      301 gacctataag agtcatatca ctgaacttca aaagcggaat gtcccagttg ctgtctacgc
      361 ttatgtagct ggaaaaagtg tccaagaaat ggaaaaggct gccgaagttt tctacaatgc
      421 tgcctctcct tacagcccta gttactattg gcttgacgtg gaagacaaaa ccatgtctaa
      481 tatgaacgaa ggtgtcgaaa actttcgagc aaaactagca tcactaggtg ctaaaaacat
      541 cggcatctac gttggtgtct acttcatgga agaacacagt attgatacag gcaagtttac
      601 ctctgtttgg attccgtcct acggctcaga ctcaggattt ttggagagca gtcctaaaac
      661 tgatttagat tatgatattc accaatacac atctaaagga aaaattgccg gctttgacca
      721 cgatttagat atcaacgtta tctctccctt aaaaaataaa gaagaaacct ttcgaaaact
      781 ttttttaaaa ccttaaaagc atttgtttat catttgcttt ttctttttgt gtctgattgt
      841 gatacaatat agtaaggatt ttttgaaata gaaatagttg gaggaaatat atgctctcat
      901 ggttagcacg cgttattaaa gggattgtaa ttgctcttgg atttatccta ccgggaattt
      961 ccggaggggt tctagcagca atcttaggaa tctatgaacg aatgattggc tttctggccc
     1021 atccctttaa agactttaaa gaaaatgttt tgtactttat tccagttgcc atcggtatgc
     1081 ttctgggaat cggcttattt tcctacccga ttgaatacct gcttgaaaat tatcaggttt
     1141 ttgtattatg gagctttgcg ggagctatta tcggtacagt tcctagcctc ctcaaagaat
     1201 caactcgaga atctgaccga gacaagattg atttagcttg gttatggaca acctttatca
     1261 tttctggatt aggactctat gccttaaatt ttgtcgttgg aaccttaagc gccagctttc
     1321 ttaacttcgt cctagcaggc gcactattgg cccttggcgt cttggttcct ggcctcagcc
     1381 catcaaattt acttttgatt ttgggactct atgctcctat gttgactggt tttaaaactt
     1441 ttgatttctt gggaaccttc tttccgattg gaattggtgc aggtgcaact ctcatcgttt
     1501 tttcaaaatt gatggattat gccttaaaca actaccactc acgtgtctat catttcatca
     1561 tcggtatcgt cctatcaagt acccttttga tcttaattcc aaatgcagga aacgctgaaa
     1621 gtatccaata cacaggactt tcacttgtcg gttatgtcat catcgccttc ttctttgcgc
     1681 tgggaatctg gcttggtatt tggatgagtc aattggagga taaatataaa taatggcaaa
     1741 aaaagttaaa atcaaaaaaa cattggtgga acaaatccta tctaaagcag ctatccctca
     1801 tcaggggatt caaatcaatg ccctagaagg agagcttcct caaggttatg aacgagatca
     1861 gattttcaaa accttggcgc ttttgggaga caagaccgga ccgattatcg gaattgtccc
     1921 tatcactcag cacttgtctg aaaagaaact agctaaaatt tctggcaata aaaaagtgag
     1981 catgattcca caaaaggact tagaaaaaac aactggttac attcatggag ccaataatcc
     2041 tgtcggaatt cgtcagaaac acaattatcc catttttatc gataaaattg ctctagattt
     2101 ggatcaaatg attgtctctg caggcgaagt cggacacagc attatcgtcg cgccacaaga
     2161 cttggctagc tttgtaaagg cagactttgt agatattttg gaggacatca agtaatgaaa
     2221 ctctactttg tccgccacgg tcgtaccctc tggaaccaag aaggtcgttt tcaaggtgct
     2281 agtggtgatt ctcctcttct tcctgaatcc attgaaaccc taaaacgact tggtcagtat
     2341 ctcaaggaaa ttccttttga tcagatttat tcaagtgatt tacctcgagc ggtcaaatct
     2401 gctgagatta tccaaagtca actctatacc ccctgttctt tagaaatcgt tcctaatctc
     2461 cgtgaatggc aacttgggaa gttagaaggt ttgaaaattg caaccttgga agctatttac
     2521 ccgcaacaaa tccaggcttt tcgttcaaat cttgctcaat ttgacactcg aatgttcgga
     2581 gccgaatccc tctatagtac aactcaacgg accatccaat ttatcaaatc attaaaagat
     2641 agtccagctg aacgtattct aattgtcgga cacggcgcca atcttactgc cagtcttcgt
     2701 actctcctag gctataaaga accacttctt cgtaaagatg gcggtctagc aaatgccagc
     2761 ctgaccatta tagaaaccca tgattttgaa acattcactc tcaatacttg gaatgatact
     2821 tcttatcaat cataattata gcctagcctt ctgaactaga accataggag atatagtcag
     2881 ttgaaacaaa aataagacaa aagaaccttg taaaaaggta ttgcaacttg ccaatacctt
     2941 tttgatatga acacgtgtct tctcaacagg attgtagagc aaaatgaaat aaga
//
LOCUS       DAVMEI010000171         2899 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVMEI010000171 DAVMEI010000000
VERSION     DAVMEI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.172"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(19..1266)
                     /locus_tag="VYQ24_001824"
     CDS             complement(19..1266)
                     /locus_tag="VYQ24_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1968924.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSAFILFLGKSKLSLSISSADGEKLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQVCVLLAIIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGFSLLAIFLTILNLKNDI"
     gene            complement(1457..2308)
                     /gene="rgg"
                     /locus_tag="VYQ24_001825"
     CDS             complement(1457..2308)
                     /gene="rgg"
                     /locus_tag="VYQ24_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1968925.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRKTPDS"
     gene            2432..2773
                     /locus_tag="VYQ24_001826"
     CDS             2432..2773
                     /locus_tag="VYQ24_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1968926.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         1009 a          573 c          524 g          793 t
ORIGIN      
        1 taagaaaaaa attaggattt agatatcatt tttgagatta agaattgtca aaaaaatagc
       61 tagcaaggaa aaaccaacaa atagcatcca agtcaactgt atattccata cggctactag
      121 tgaaaaacaa gctgttccca caggtatgga taaggtaaac aatagaccta aaaaattact
      181 agtacgagct agaacctctg gagctagatt tttcatgagc atggcactaa tctttggttg
      241 aactttacca gacacataca gagtaaagaa gagaaatagc aaaccaagca cgacttgatt
      301 gaataaatta gccaaaccaa ctagactaag tcctacggtc tcccacatca tcaatctagg
      361 caaggactgc ttcccaaaat aatcattgcc cgtaaggcta ctaatgatga ttgctaacaa
      421 aacacagact tgattgataa atagtgcctc tgtataagaa aaattcaaga gagaatggct
      481 caaaaagaag atattataaa ttccacccaa agcgccaccc aaggaattaa taagcaagac
      541 agcaaagagc ataaaaccaa agtttttctg tccactttta agaaaaacga gacgtaaatt
      601 tcggtaaatt gttaggaact ggtctttgat agaaagcttc tcatttttta gtttttcacc
      661 atcagcagat gaaattgaca ggctcaattt gctttttcct aaaaaaagaa taaaggctga
      721 tacgaggaaa aagcaagcat taattcccgc aacgagggaa aaattgttga ccgatagagc
      781 taagagccag actccgaaag cttgaccacc aatagctgaa atataggtga tgaactgtga
      841 aaaagaataa gcctccatca gatcatcttc agctactttt tccttaataa gaggcatacg
      901 caggccacct gcaaaatcac tgatgatatc actaatgaca ttgatcaaac acaggctaga
      961 aaaggcaaag agactagctt gctgaacaac tagggctgct agaaaaaata gaaccgcctg
     1021 aaacaaaccg ctatagacca tccatttgac cttgtccctc gtgtaatctg cccgaatccc
     1081 tacaaaaact gtaaagaggg tcggaagaat catgacaata ttcgccatag caacagcaaa
     1141 agatgcttgt gacaaggtcg atgcatagac gataaagacc aggttgaaaa tcgaagcacc
     1201 aaaagcattg aagaagcgtg ataaagtcaa aattctataa gctggatttc taaacaatag
     1261 tttcatagat aatctccctc cttgttctaa gttctacatc aagaatagct ttagtatagc
     1321 acttatgaat ttgcgaaacg attcttttga atatatgcaa caaacaaaat gagcaaaaaa
     1381 acgatagaac ttttgtaatg acctgagaga gggcaacagt tctatcgctt caaatttttt
     1441 cttggtttgc agatattcaa gaatcgggag tttttctata gtattcggca gatttattac
     1501 agccaagcat ctcaaaaata cggacagcat cctccatctt tttctggcct tccttgactc
     1561 taccttgctt gctatcaagg agaccttctg cccacagata aacaattcgg aaataggtct
     1621 cattttcctt gtagaaatgc tcttcgataa cacgtttaaa ataataggca ttggtaaatt
     1681 cttcacactc aatactagct aaaaagccat tcaatagtat agtatgaaaa aggtttcgat
     1741 tgccagacat ttccattaga aaatcagatt tacgtaccat ttctcgtaca tatctagtaa
     1801 aaagagaaac agataaaaat ggagaactga ctgaaaataa attgagttca tagattcccc
     1861 agatctcggt agaaaacaaa taatcatgaa ggacttttcc ttcctctgct gttaagtcta
     1921 ccctttcatc tatgctcttc atataagact tgataataat ggcatttaga atatgtttct
     1981 gtttgttgtg agaatgggca tgcttttgat actccctgcg atataagtcc tcaagaggtg
     2041 ctatattctt tggttccaag acatctgtaa tttcttttct caactcagaa tctgtatcat
     2101 actggaaacc tctcgccaga aagaggatct cctccacact ggcagatata ttttccagag
     2161 caaatagaaa cttttccacc gaaagctcac tctgacctgt ttcaaaacgg gacaacatag
     2221 acggcgaaaa ttgtcctccg gttgcttgtc tcagtgagat atttcttgac tctcgtaatt
     2281 gtctaaagac ttttccaatc tgctccatag acttcccctt gattccgtat tttcttcatt
     2341 ttatcatatt tttcagaaaa ttcatcaaaa acttgccaaa ttgtcagaat tatgagaaaa
     2401 tagaggatat ttatcacgtg gagggactgc tatgagagac gatatcaaaa tcaatgaccg
     2461 tgctttggcc ttgcaagacc aaatcatcga aaaactagag aaagtttttg atacagatgt
     2521 ggaattggat gtttacaatc taggtctgat ttatgaaatc aatctggatg aaacggggct
     2581 ctgcaagatt gtcatgacct tcaccgatac tgcctgtgat tgcgccgaaa gcctgcctat
     2641 tgaaatcgtg gcaggtctga aacaaatcga gggtatcaaa gatatcaagg ttgaagttac
     2701 ctggtcgcct gcttggaaaa tcacacgaat cagtcgctat ggccgtattg cccttggact
     2761 accacctcgt taagcaggcc aatcattttt aaagatgaaa aaagcaagcc aaaaatggct
     2821 tgctttttga tactcaatga aaatcaaagg gcaaactaga aaactagccg caggttgctc
     2881 aaaacactgt tttgaagtt
//
LOCUS       DAVMEI010000172         2880 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVMEI010000172 DAVMEI010000000
VERSION     DAVMEI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.173"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            40..>2880
                     /locus_tag="VYQ24_001827"
     CDS             40..>2880
                     /locus_tag="VYQ24_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1968927.1"
                     /translation="MLCFGLLAGVKADNRVQMRTTINNESPLLLSPLYGNDNGNGLWW
                     GNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDTKELREWYVKMLEEAQSLNI
                     PVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENYWIYNNQLAPHSAKYLEVCA
                     KYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLATKNTPIRDDAGTDSIVSGF
                     WLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYASEPESMIAMEMMNVYTGGG
                     TVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAPSKEEVVNRTKAVFWNGEGR
                     ISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKEKISSIFPNAKILTKNSEEL
                     SSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINKNQQVMLPMYTNNTKSLSLD
                     LTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDASKSWKKEELELANWISKNY
                     SINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTENWDENTHVYTITVNHNGMV
                     EMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVDYNGDPNRAVDGNRNGNFNS
                     GSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQRLSNFDVILYDNNRNEVAKK
                     HVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVEVFRESDGKQSEEDIDKITE
                     DKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVTHTKADSNAWWQVDLGEEFT
                     VSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDKVVDGLLSLKVLSVGAKLVK
                     IELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQSSTDYNGFSRLAVDGNKNGD
                     YGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIY"
BASE COUNT         1018 a          396 c          606 g          860 t
ORIGIN      
        1 aaaagaaaat taatcacaat actgtttgta tgtattggga tgttatgttt tggattgtta
       61 gcaggagtta aggctgataa tcgtgttcaa atgagaacga cgattaataa tgaatcgcca
      121 ttgttgcttt ctccgttgta tggcaatgat aatggtaacg gattatggtg ggggaacaca
      181 ttgaagggag catgggaagc tattcctgaa gatgtaaagc catatgcagc gattgaactt
      241 catcctgcaa aagtctgtaa accaacaagt tgtattccac gagatacgaa agaattgaga
      301 gaatggtatg tcaagatgtt ggaggaagct caaagtctaa acattccagt tttcttggtt
      361 attatgtcgg ctggagagcg taatacagtt cctccagagt ggttagatga acaattccaa
      421 aagtatagtg tgttaaaagg tgttttaaat attgagaatt attggattta caataaccag
      481 ttagctccgc atagtgctaa atatttggaa gtttgtgcca aatatggagc gcattttatc
      541 tggcatgatc atgaaaaatg gttctgggaa actattatga atgatccgac attctttgaa
      601 gcgagtcaaa aatatcataa aaatttggtg ttggcaacta aaaatacgcc aataagagat
      661 gatgcgggta cagattctat cgttagtgga ttttggttga gtggcttatg tgataactgg
      721 ggctcatcaa cagatacatg gaaatggtgg gaaaaacatt atacaaacac atttgaaact
      781 ggaagagcta gggatatgag atcctatgca tcggaaccag aatcaatgat tgctatggaa
      841 atgatgaatg tatatactgg gggaggcaca gtttataatt ttgaatgtgc cgcgtataca
      901 tttatgacaa atgatgtacc aactccagca tttactaaag gtattattcc tttctttaga
      961 catgctatac aaaatccagc tccaagtaag gaagaagttg taaatagaac aaaagctgta
     1021 ttttggaatg gagaaggtag gattagttca ttaaacggat tttatcaagg actttattcg
     1081 aatgatgaaa caatgccttt atataataat gggagatatc atattcttcc tgtaatacat
     1141 gagaaaattg ataaggaaaa gatttcatct atattcccta atgcaaaaat tttgactaaa
     1201 aatagtgagg aattgtctag taaagtcaac tatttaaact cgctttatcc aaaactttat
     1261 gaaggagatg ggtatgctca gcgtgtaggt aattcctggt atatttataa tagtaatgct
     1321 aatatcaata aaaatcagca agtaatgttg cctatgtata ctaataatac aaagtcgtta
     1381 tcgttagatt tgacgccaca tacttacgct gttgttaaag aaaatccaaa taatttacat
     1441 attttattga acaattacag gacagataag acagctatgt gggcattatc aggaaatttt
     1501 gatgcatcaa aaagttggaa gaaagaagaa ttagagttag cgaactggat aagcaaaaat
     1561 tattccatca atcctgtaga taatgacttt aggacaacaa cacttacatt aaaagggcat
     1621 actggtcata aacctcagat aaatataagt ggcgataaaa atcattatac ttatacagaa
     1681 aattgggatg agaataccca tgtttatacc attacggtta atcataatgg aatggtagag
     1741 atgtctataa atactgaagg gacaggccca gtccctttcc caacaccaga taaatttaat
     1801 gatggtaatt tgaatatagc atatgcaaaa ccaacaacac aaagttctgt agattacaat
     1861 ggagacccta atagagctgt ggatggtaac agaaatggta attttaactc tggttcggta
     1921 acacacacta gggcagataa tccctcttgg tgggaagtcg atttgaaaaa aatggataaa
     1981 gttgggcttg ttaaaattta taatcgcaca gatgctgaga ctcaacgtct atctaatttt
     2041 gatgtgattc tatatgacaa taatagaaac gaagttgcta agaaacatgt taataatttg
     2101 tcgggtgaat ctgttagtct agatttcaaa gaaaaaggag caaggtatat taaagttaaa
     2161 ttactaacga gtggagtgcc tttgagttta gcagaagtag aggtttttag agaatcagat
     2221 ggtaagcaat ctgaagagga tatagataaa ataacagaag ataaagtagt ctctacaaat
     2281 aaggtagcta ctcaaagttc aaccaattat gagggtgtag ctgctttagc agttgatggt
     2341 aataaagatg gagattacgg acatcattcg gtgactcata ctaaggcaga ttctaacgct
     2401 tggtggcagg tcgatctggg agaagagttt acggtttcta aagttgatat ttataataga
     2461 acagatgccg aacctcagcg tttatctaat tttgatgtta tttttctatc ttcatcagga
     2521 gaagaagttt ttagaagaca ttttgataaa gtagttgatg gtttgttatc tttaaaagta
     2581 ctttctgtag gggctaagct agtcaaaata gaattaaaat cagcagctat tccgttaagt
     2641 ttagcggaag ttgaagtcta tggttcaaag agaactccga agaaactttc taatattgca
     2701 ttaaccaaag aaactcgaca gagttcaacg gattacaatg gtttttctcg tctagcagtt
     2761 gatggaaata aaaacggaga ttatggtcat cattcagtga ctcataccaa aggagattct
     2821 ccttcatggt gggagataga tttagcacaa accgaagaat tagaaaagtt aattatttat
//
LOCUS       DAVMEI010000173         2868 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVMEI010000173 DAVMEI010000000
VERSION     DAVMEI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.174"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(52..891)
                     /locus_tag="VYQ24_001828"
     CDS             complement(52..891)
                     /locus_tag="VYQ24_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1968928.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1033..1881
                     /locus_tag="VYQ24_001829"
     CDS             1033..1881
                     /locus_tag="VYQ24_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1968929.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(1974..>2868)
                     /locus_tag="VYQ24_001830"
     CDS             complement(1974..>2868)
                     /locus_tag="VYQ24_001830"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1968930.1"
                     /translation="NQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAARKDLLPIV
                     VMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQFASLTAQEF
                     FATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKGKISSTRIR
                     QAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYMPADGVYVV
                     DVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLDRIRDMTKF
                     DSVDQLVDQLKADEEVTRNWS"
BASE COUNT          866 a          598 c          464 g          940 t
ORIGIN      
        1 cccttttagg tcaagttaaa aacgctattt cttcccattt tatttatttt tttaggagtg
       61 gtaacgtatc aaaatagccc aagcgttctc acccgtgtga gtttgaataa tggaacccgt
      121 ttccaaaaca gaaattggct tttcaacata agcttgtaag ctttctttca tctcttttgc
      181 ccaatcatca ctaccagaat atgaaattcc aatctctgct acagcacgtt cagaaagcga
      241 tgttatcaac tcatctaacc attttttaaa tgttttagtt ccacgacctt taaccattgg
      301 ctgcaattca tggtctttca tttgcatgac aacacggata ttgagaagag agctcaacaa
      361 gccagttaca cggctaattc gtccaccttt gacaagattt tccaaagttg aaacaccaat
      421 ataaagctct gtatggtttt taacctcttc tacatgagat aaaattgcct ccatatcttt
      481 accttcttga gctaacttcg cagcctcaac aacttggaat ttcagggctt ggtcagtgaa
      541 ggaactatca acaacagtca catctgcagt agataggcta gcaccttggc gtgctgcttc
      601 taccgtaccc gaaagagcat gggacatatg aatagcaaga atctggccac catctttgca
      661 taggtcttca aaaatctcag caaagacacc tacaggtggc tgacttgttt tcggaagatt
      721 cttactttct tgcatcaact gaagaaattt accttcttct ttcaaatccg catcagaata
      781 aacaacatta tcaatcatta cagataatgg aacaattgta atatctaatt gctttactag
      841 ttcaggttca atagtaacag atgaatcggt tacaatctta atttttgtca tagtatcaat
      901 ctttctattt taggattcag attggtttcc ttacttctaa ttatatcaaa aaaaagatta
      961 aaaatcctaa tggagtcaat caaattttcc gtaaaatttg atataatcaa cttataagaa
     1021 aagaggtgtc ctatgattaa aaaaatttac cccattttta ccattttact aggtgctgct
     1081 atttatgctt ttggactgac ttattttgta gttccccatc atctctttga aggaggggcg
     1141 acaggcatta ccctcatcac cttttatctt tttaaaatcc ctgtttccct catgaacctg
     1201 ctgattaata ttcccctttt catcctagct tggaagatct ttggagccaa atccctctat
     1261 tctagtttac taggaacctt agctttgtcc gcctggttag ctttttttga gcatattccc
     1321 cttcatattg atcttcaagg tgatttacta atcacagccc ttatagcggg aatcctattg
     1381 ggaattggcc ttggaattat ttttaatgct ggaggtacaa ctggcggaac tgatattcta
     1441 gctcgtattc tcaacaaata cactcatata tccataggaa aactgctctt tatcttagat
     1501 ttttgtattc tcatgttgat tctcctaatc ttcaaggatt tgagattggt ttcctacacg
     1561 cttttgtttg attttattgt ttctcgtgtt attgatttga ttggtgaagg aggatatgcc
     1621 ggcaaaggct ttatgattat cacaaaacgt cctgaccaac ttgctaaggc gattaatgat
     1681 gacctcggaa gaggtgttac ttttatttct ggtcaaggct actatagtaa agaaaatttg
     1741 aaaatcatct actgtattgt cggaagaaat gaaattgtga aaacgaagga aatgattcat
     1801 cgaatcgatc ctcaagcctt tataactatt acagaagccc atgaaatcct aggagaaggc
     1861 ttcacctttg aaaaagaata aaaagaggta atgtcgtgac ctcaaaagtt agactaaatc
     1921 atctatcttt tgggttacag acaacctctt ttttgtttta tttactcaag ctcttaagac
     1981 caattccgag ttacttcttc atcagccttt aactgatcca ctaattggtc aactgagtca
     2041 aatttggtca tatcacgaat gcgatcaagc caataaacca tgacggtttc cccataaata
     2101 tcttgattaa aatcaaaaat attgacttca aaacgtgctt cttctccatc aaaggtcaca
     2161 tttttcccga cactagccat agcacgatac ttctgtcttt gaatctcaac atcaacgaca
     2221 taaacgccat ctgctggcat ataagtacgg tctaaaagca ctaaattcgc tgtcggataa
     2281 ccaattgtac gaccacgagc attaccatga accaccatac ctcttgatgg aagcggtgcc
     2341 cccaaaagtt ttcctgcttc tttcacattt ccatctaaaa tagcttgacg gatacgagtt
     2401 gaactaatct ttcctttctc atcttctaca ggtggaacaa tgataacttc tccatcaaag
     2461 taatccttta aatcttctgc tgtttttttg tcagaaccaa atgtataatc aaaacctgca
     2521 acaataattt tggcattcat agccttgata taagttgcaa agaattcttg tgcagtgaga
     2581 ctagcgaatt gactactaaa atcaaggaga tataattctt ctacaccttc gcgctttaat
     2641 tttctttcac gttcagcagg gttcaaaata tgcaaaaaca aatctggatg ataaggctct
     2701 aaagcgatct ttggagattc attaaaggtc ataacgacga taggcaataa atcctttctc
     2761 gcagccttgt tggcaacacg aaataattct tgatgcccct tatgtatgcc atcaaaatag
     2821 ccgagaacaa cgactgaatc agatggtgtg ccaatatctt tttggttt
//
LOCUS       DAVMEI010000174         2860 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVMEI010000174 DAVMEI010000000
VERSION     DAVMEI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.175"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(9..2267)
                     /locus_tag="VYQ24_001831"
     CDS             complement(9..2267)
                     /locus_tag="VYQ24_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1968931.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2496..2726)
                     /locus_tag="VYQ24_001832"
     CDS             complement(2496..2726)
                     /locus_tag="VYQ24_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1968932.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
BASE COUNT          766 a          589 c          596 g          909 t
ORIGIN      
        1 tataggattt atttttcttt ttcagaactt ttaacttcag cttttttggc agatttaatc
       61 tgaatgtttc ccttgctagt cataactgct ttgagatctt tttcgcttgg attttcaagg
      121 tagtagtcag tgattgtgtc ctcaatatag tcttgaatag tccgacgaag tgggcgtgct
      181 cccatttttg gatcataacc taggtcaacc aacttttcct tgaccttgtc agttacatcc
      241 aaacgaatgt tgttgctaga gaggcgcttg ttaacatctg ctagcatgag ctcgacaatc
      301 tgaaggaggt tatccttgct gagagcctta aattcgataa tgccatcaaa acggttcata
      361 aactctgggc taaagaagtt accgagttca ccgaggacag aattggtacg tccttctcta
      421 gcagcaccaa atccaacgct agcttcggtc tttcctgtac ctgcatttga ggtcatgata
      481 atgatggcat ccttgaagct aacggtgcgt ccttgcccgt ctgtcaaacg accatcgtcc
      541 aagacttgaa gaaacatgtg cataacatct gggtgagctt tttccacttc atcgagaagg
      601 atgagagaat acggattgtg gcgaactttt tcagttaatt gaccagcctc atcatagcca
      661 acataacctg gaggagcgcc gaccaactta gccacactat gtttttccat gtattcactc
      721 atatcaaagc gaatcatact atcagcagaa ccaaaaagtt cgatagccag ttgtttggaa
      781 agttctgtct taccgacacc agttggtcca acgaagagga agcttccgat tgggcggtta
      841 ggggtaccaa gtccgacacg attacggcga atagccttgg caatcttatc gactgcatca
      901 tcttggccaa taacatgaga cttgagatct tcggctagat ggatgagttg agattgttct
      961 ttctctttca aatcaccaac agggatattg gttttctgct cgataatgtg ctcaatagtt
     1021 ttctcgctga tgataggagt atcctggtct gtgatctttt tcttttgcat ttccttatac
     1081 ttggcaatct ggtcgcggaa gtaggccgcc ttctcaaaat cttcttctcg tgtagcttga
     1141 gacttgagat tttcagcctc aatcaagcgc tgatcaatta ctttaggatc cacaaaattc
     1201 aaggtcaagt tcatcttaga accagcttca tctaggaggt caatggcctt gtcaggcaag
     1261 aagcgatctt ggatgtagcg attggaaaga gttgcagctg cttcaatcgc agcatcggta
     1321 tattgaacgt ggtggtaatc ttcgtatttc ttttgaatcc ctttgagaat agtgattgtt
     1381 tcatccaccg ttggttcatc gactttaaca ggctgcatac gacgctcgag ggcagcatcc
     1441 ttttcaatga tacggtattc attgagggta gtagcaccga ctagttgcag ttctccacga
     1501 gcaagggctg gcttgaggat atttcctgcg tccatattac catcactcgc agaaccagca
     1561 ccaacaattt catggatttc atcgataaag aggatgatgt cttcacgttt gcgaatttct
     1621 tccatgagtt tttgcatgcg ttcttcaaat tgtcctcgaa tccccgttcc ttgaactaag
     1681 ctaaccacat ccagacggat gacttgttta ccttggagtt tatgtggcac atcgccatcg
     1741 acaattttct gagctagacc ttcgacaacg gccgtttttc cgacaccagg ttcaccgata
     1801 aggacaggat tattcttggt tctacgattg agaatctcga tgacacggat aatctcatcg
     1861 tcgcgcccaa taacggggtc aatgtctcca cgacgggcaa tttcagttac attaatacca
     1921 aattcttcca gcaggccttt ttcttggcta ggtggcggag tttgagcaga tccacgattt
     1981 tgggaaccat aaccgccgtt tccaccgtaa cctccacctg attgggttgg gggaatagga
     2041 ggagtattgc tagaaggtct gaaattgttt agatcattga agaaatcacc aaagggatcg
     2101 aagtcacgat tgttcagatc cgtcatacct ttgaagaggc tattgttagg atctgttttg
     2161 ataatcttat agcagttttg acagaggtca atttgttttt gttttccatt gagattggtg
     2221 taaagatgaa ttgttgagtc gttaatttta cagttttgac aaagcataca tctacctcat
     2281 ttctttcttt agcctgactt gtttaaaggt caaaaatagt caaattctat actctcatta
     2341 taacaggatt ggggtgtaaa gcaagtaaaa agattgaagc tttgagaagt tgttacaatg
     2401 aaaatttttg tgaatttgga ctataaaaaa atcctatttg tgagacaaaa aggattttac
     2461 ttagacatgc agggacaatc ccaacttgtt ttagattagt agagaagttc gtaaatttcc
     2521 attgcgatta agtcaatgct atcaaacgta tacgtttcgc gatcttctac gtcacgaagg
     2581 gtaaagattg ggccgttttc ttcgtcgtgg ctgtacgcaa cttctgcaac aacaactcct
     2641 tcgcgttcaa aattacgttt taaatttccg ccatcgtttg ccattgcttc aaggcggttg
     2701 atgattctaa ccaaatgtga ttccattttg attctccttc atttcttatc gttactattt
     2761 taccataaag gagggcaact tgactagaaa aaactaagat ttctcgctaa ttctgctagt
     2821 ttaaagattt ttaaataaat tggataaaaa atttaaattt
//
LOCUS       DAVMEI010000175         2860 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVMEI010000175 DAVMEI010000000
VERSION     DAVMEI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.176"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>2860)
                     /locus_tag="VYQ24_001833"
     CDS             complement(<1..>2860)
                     /locus_tag="VYQ24_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1968933.1"
                     /translation="EKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQETAGVHY
                     KYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSKNERQAL
                     VAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLLNYNTDY
                     ELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDYNVTPNF
                     VDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQLAEHKNL
                     ETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEIQENPDL
                     AEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTKKAQVIK
                     EQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKGVPEVQP
                     ALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPEQVAPLP
                     EYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEP
                     SKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPSAEDTKP
                     NNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHNFINNST
                     DNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVIKLPETT
                     GREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQYQGGNP
                     GMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVYDELYRH
                     KDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENYFMKVKS
                     ENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHEN"
BASE COUNT          717 a          596 c          457 g         1090 t
ORIGIN      
        1 ttattttcat gttgtatcag actatttgca cttgctacta tcttataaac atctctatta
       61 tctttagtat cattagtaat gcttgtaact ggtagcatca catctttaaa gttctctgat
      121 ttaactttca taaagtaatt ttctgcatta cttggtaaag aagcaagtga agttacatgt
      181 cgatacttat taccatcttt tgaatatagt tctacagaag atatatttct aagttctaat
      241 ttcttaataa cttctttttc atctttagcg tcatcttttt tctcatcttt atgacgataa
      301 agttcatcgt atactggtgg aatttcatta cctgctataa tactcttaat ccaagctaat
      361 tgtgtaggag ataaaatcaa tccgcctgag cgattttgtg ctccattttg atgcacacca
      421 ataacttcac cattagcatt aaatattcct ccaccactca taccaggatt tccaccttga
      481 tattgaacac cgtaggtgtt attcgcatat tcttgttcca tttctaccgt agcatctttt
      541 aagtgcgtat atttcccagc tggatatcca tgaacattta gtttgtcatt aagatttact
      601 ttcgaataat ttttaactaa ttctacatgt tctctgccag ttgtttcagg tagcttaatc
      661 actgctaaat catagataaa tccttttgga taatcttttt tattataaaa atgaatattg
      721 ttagcagtaa atgttccttt ttgtccatca ggagtcaccc actcatatac gtttttagat
      781 ttatcacctc taaattcgcc agtagtatta tcagtagaat tattaatgaa attatgtgct
      841 actgttaaaa gtacatttgg tgcaataaaa gtagctgatc ctactccagt aaccttcgac
      901 gaatctgtaa taaagttcat attaattaat gcactagatt ttttctgaat actcatttct
      961 tctggtgtat tagtattttc attatttggt ttagtatctt ctgcactagg ttgttcgatt
     1021 ttaccagtat attctttcag tgcttctact ttctctggct cgactatcgc tccggcttgc
     1081 acccctgtgt attctggtag tggtgctatc tgctctggtt caacaatagc gccagcctgc
     1141 acccctgtgt attcttttga tggttctact ttttctggtt ctactattgc tccggcttgc
     1201 actcctgtgt attccttcgg tgcttctacc ttctctggtt cgactatcgc tccagcctgc
     1261 actcctgtgt attccttcgg tgcttctact ttctctggct cgactatcgc tccggcttgc
     1321 acccctgtgt attctggtag tggtgctacc tgctctggtt caacaatagc gccagcttgt
     1381 actccagcat attccggttc aactttttca ggttcaacaa tagctccagc ttgtactcct
     1441 gtatattctg gtagcggttc tacctgctct ggatcaccct tgtcagtcat gactgcctct
     1501 ggtaacgctg gttgaacttc tggtacgcct ttatcactta ctacagcttc tggcaactct
     1561 ggttggactg ctgactcacc tttgtcggtt aatacagctg ctggtagttc aggctgaatt
     1621 gcgggttcaa caatagctcc agactgtact tccttatgtt ctgcaccagt ctcagcttgt
     1681 tcctttataa cttgagcttt tttagtacct ttttcgacta ttcttgaaac aggcgcagtc
     1741 gttgaagttg aaataatttc tcgtgaaact tcttcattgt ttacagaaaa tattctgacg
     1801 atttcaactt tcttacctaa tgcaccttct tgttttactc ttacagttcc ttctgctaaa
     1861 tcaggatttt cttgaatttc ttcttgaaaa tctatttttg tttctatagt ttcgtcacga
     1921 tataagagtt ccggtttgtt caactgacct gatagcactt catcctgtgg atttaatgta
     1981 tttaccccag tcttttcctt tggagaaatc ttctcctctt tcttcgtttc tagattctta
     2041 tgttcggcta attgttcttg agaatctgaa gattgtttct cttcttttct tggattgatt
     2101 aattcagtag agaaaggttt ttcaactact tgaacttctg tcggcttagt tgaagaaaca
     2161 ggtgtttgtt cctgaatagc ttgtactgtt gatggatggt ctacaaaatt tggtgtaaca
     2221 ttataatcca ccttttgttg ttttgtagga gtggctgctg atttttcttg attacttact
     2281 tcaaaatcag aagtcgtttt tccctctttg atatatccaa tataagtgta acctgaaatc
     2341 tctttaggaa gaggtaattt ttctccagag gtcaattcat agtccgtatt gtaatttagc
     2401 aaaagatgat tttctaaagc atggactgaa actaagacac catttcccat tcccgcaacc
     2461 aatactaaat gtaataccgt tttattctta acctttttct tggaaacagc aaaaattaaa
     2521 attcccagag cagctaagct agcaccagca actagggctt gcctctcatt cttgcttcca
     2581 gtatttggta attccgccag ttgattttga gaatttaact tataaacaag ataataagtt
     2641 tcatcatcat tctccacgta tgtcggaata tcatagacaa gctgcttctt ttcttctgat
     2701 gatagctctg aatctgccac atatttatag tgaactcccg cagtttcttg agcatccaca
     2761 gatgaactag ctaatacaga cataaaaaat aaacttgaaa tcgttgcaga tacaagtcct
     2821 actgataatt ttctaaatga aaaacgctct tgtttttcac
//
LOCUS       DAVMEI010000176         2853 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVMEI010000176 DAVMEI010000000
VERSION     DAVMEI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.177"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(307..1617)
                     /locus_tag="VYQ24_001834"
     CDS             complement(307..1617)
                     /locus_tag="VYQ24_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1968934.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            1854..2726
                     /locus_tag="VYQ24_001835"
     CDS             1854..2726
                     /locus_tag="VYQ24_001835"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1968935.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          862 a          492 c          660 g          839 t
ORIGIN      
        1 ctatcctgat ctatttgtcc tgttcttatt tcatttcact atatctcaaa ttgagtatga
       61 cgaagtgcgc tcccatgtcc tgggaacgca ctttcttcat atttttcata ttcttgaatc
      121 catcgataaa gactattggg atgaattttt aaagttgaac taatcatttt tacaggatga
      181 gatttacagc agagagtttg aaggctttat caaaggtttt tcttggcata atgacttttc
      241 ctcgtttcca cttaattttg tgtctacttt attataccaa gtccaccctt aagttagata
      301 ataaatctaa cttaaggaag ctagaaggat gagaatccag gtggtcaaga gtcccaaact
      361 taagctgatg gggacaccca gaataatttg ctttttgaag gcaaggccac gttcctctat
      421 attgggaagt gagagttgaa tgagagaacc agctgatgaa aagggtgaga tattagtaga
      481 tagagcgcca ataacggtgg ctgttgtgag taagtgaata tcaatctgag gattttgagc
      541 actgatgata gcaatgatgg gaaagagggc tggagctaca acggataggg tggaactaaa
      601 gagtgacatc actccggcta tcacacaaaa gaacagaggt aaccagaaat gaggaatggt
      661 tgttgtcatg aggtgcccta tcagtgtgac taaacctgac ttgaccgcta gagacattag
      721 taagctcatg ccgcagagca tgataattgt agcccaggga accttagcta aaatggcttc
      781 ttgcttccct aatttgagcc ttaaggcgag gcagaccatg agtattgaga caaagccaat
      841 atcaaatgtt ttttgataag tagctatcca ggcgatgttt gggaaaatga gatgcaacaa
      901 gggaaaaagc caaaccaaaa ccatgctgct gatcatgagc aaggtggttt gtctttgaac
      961 cttgctgagg agtggtggtt ggtcaatagt caaggatgag tttgttcttc ccttactata
     1021 gtgactgtaa caggataata aaagcaagac gatgagtggg tagataatgc tgacgataaa
     1081 gatatgattg ccaagtgaaa aagcttgctc ttcccatccc atttgcttaa acaggccttg
     1141 aaagacaatg cctgagctac tggttatcaa attagcccct cctgaagctc cccaattgac
     1201 ggcttgagct ccaatcaaag ggtgtttgtc cgctttttga cagagggtaa tcgctagagg
     1261 acagcaaacg gccatagtag tgaaaaatcc agcacctaaa gcagacaaaa gggttgccat
     1321 caggtataaa atcatgtaga gggcgttagg gtgggtgcgt gtgcggtaga gaatgtgttg
     1381 agccaaaaca tcaagagtac cgttagttgt tgcaacgtta taaaagagag agacgctaaa
     1441 aatggtaaaa aagagtgagg ttggccaaaa atgaagaagt tctttggggc ttaatcccat
     1501 gagagtggtt gcgatgaggt aagaaaaagc aatagccagc aggccaatat tgattttggt
     1561 gcggtaacca attccaatgg ctagagcaat ggcgctaatc attattaaat gaatcattga
     1621 attgtccttt cagttagaat atggaaatag gttggcaatt ttggcgctca aaagaccacc
     1681 tgtttcctat tggtacttga ctattataca caaaaaaaat acaattgtct tgacaattgt
     1741 attgacactt gttagaatta aaaatgaggt attcaggctt gtgatttcct atggaagtta
     1801 atagtaattg cctctagtgc ttacaagtga tattaaaaat agaggactta gtgatgtcaa
     1861 tcatttcaac tgatttaacc ccttttcaaa tagatgatac attgaaagca gccttgcgag
     1921 aagatgttca ttccgaagat tacagtacca atgccatttt tgatcatcat ggccaagcca
     1981 aggtgtcgct ttttgccaag gaagctggtg ttttagcggg actaaccgtt tttcaaaggg
     2041 tttttaccct atttgatgcc gaggtgacct tccagaatcc tcatcaattt aaggatgggg
     2101 atcgtttgac tagtggcgat ttggttttag aaatcatagg ctcggtgaga agtctcttaa
     2161 catgtgaacg cgttgccttg aattttttac aacatttatc agggattgct tcgatgacag
     2221 ctgcttatgt agaagcctta ggcgatgatt gcattaaggt atttgatact cgaaaaacta
     2281 ctcctaattt acgtcttttt gagaaatatg ccgtgagagt tggcggtggc tataatcatc
     2341 gctttaattt atcagatgct atcatgctaa aagacaatca cattgcggca gtaggtagtg
     2401 ttcagaaggc aattgctcaa gcgcgtgcct atgccccttt tgtgaaaatg gtcgaggtgg
     2461 aagtggaaag ccttgctgct gccgaagaag ctgcggcggc gggtgctgat attatcatgt
     2521 tggataatat gtcattggaa cagattgaac aggccattac cctaattgca ggacgttctc
     2581 ggattgaatg ttctggaaat attgatatga ccactattag ccgttttcgt ggtttagcga
     2641 ttgattacgt ctccagtggt agtttaaccc atagtgctaa gagtcttgat ttttccatga
     2701 agggtttaac ctaccttgat gtctaagttg taaaataaac taacttttta aaggatgtct
     2761 ttcctctaga acgagtttta tgtcagatag tttaaacgcc tcttcaaata tagtaaaatg
     2821 aaccaaaaat agtacacaat gtggtataat ctt
//
LOCUS       DAVMEI010000177         2769 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVMEI010000177 DAVMEI010000000
VERSION     DAVMEI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.178"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            6..554
                     /gene="amaP"
                     /locus_tag="VYQ24_001836"
     CDS             6..554
                     /gene="amaP"
                     /locus_tag="VYQ24_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1968936.1"
                     /translation="MCFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNSVVEFYL
                     ARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFVRSLVSD
                     HRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFGIERQVK
                     LEVAVKNYQPKPQNKKTVSRVK"
     gene            566..736
                     /locus_tag="VYQ24_001837"
     CDS             566..736
                     /locus_tag="VYQ24_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1968937.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            775..1383
                     /locus_tag="VYQ24_001838"
     CDS             775..1383
                     /locus_tag="VYQ24_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1968938.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1414..1617
                     /locus_tag="VYQ24_001839"
     CDS             1414..1617
                     /locus_tag="VYQ24_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1968939.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(1727..2005)
                     /locus_tag="VYQ24_001840"
                     /pseudo
     CDS             complement(1727..2005)
                     /locus_tag="VYQ24_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2203..2490
                     /locus_tag="VYQ24_001841"
     CDS             2203..2490
                     /locus_tag="VYQ24_001841"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1968940.1"
                     /translation="MIVSLKTKSRKAKDIAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
BASE COUNT          958 a          443 c          511 g          857 t
ORIGIN      
        1 agaaaatatg tttcattatt ttctgtattt taatcttgac aattttcctt cctgttttga
       61 tagattatca tcaagttagt gatctaggta ttcatctact tagctggaga cagaactccg
      121 tagttgaatt ctatcttgct agatatgtct tttgggggac agtggttcta tcaactttag
      181 ttttattatc cattttagtt gtgatgtttt atcctaaacg ttacttggaa atccaacttg
      241 aaactaaaaa cgatacatta aaattaaaga attcggcaat cgaaggtttt gttagaagtt
      301 tggtgagtga tcatagattg atcaagaacc caactgttca tgtaaattta cgaaaaaata
      361 aatgtttcgt tcatgtagaa ggtaaaattc ttccttcaga caacatcgct gacagatgcc
      421 aaataattca aaatgaaata actaatggat tgaagcagtt ttttggtatt gagcgtcaag
      481 taaaacttga agttgcagta aaaaattacc aaccaaaacc tcaaaacaaa aagactgtta
      541 gtcgtgtgaa gtaaggaagt aaaaaatgga atggcttaaa caatatcgat atccaattat
      601 cgctggtctc ataggcgtat ttctggcttg tttgattgtc tcctttggct tcttcaaaac
      661 aatatttgta ttgattttag gagcactggg agttgcagct ggattatata tcgaaaaaaa
      721 ctatatagat aaataaaaaa ataaaaatta ctaatttaat taaaggagtt tcatatgtca
      781 aacgaaaaaa acacaaacac taacgtagaa aagaaagatg ctactgttgt agctcacgaa
      841 atcaaagggg aacttactta cgaagataaa gttatccaaa aaatcattgg tctttcacta
      901 gaaaacgttt caggtctttt gggaatcgat ggtggtttct tctcaaatct taaagaaaaa
      961 atcgttaaca gcgatgacgt aacaagtggt gttaacgtag aagttggtaa aacacaagtt
     1021 gcagttgact taaacgttat tgttgagtac caaaaaaatg ttccagcttt atattcagaa
     1081 atcagagaaa tcgtatcttc agaagttgct aaaatgactg acttggaaat tgttgaaatc
     1141 aacgtaaacg ttgtcgacat caaaactaaa gaacagcatg aagcagactc agtaagcctt
     1201 caagatcgcg tatctgacgt tgctgaatca acaggagaat tcacttcaga acaattcgaa
     1261 aaagctaaat ctggtcttgg atctggtttc tcaactgttc aagaaaaagt tagcgaaggt
     1321 gtagaagctg ttaaaggtgc agcaaatggt gtagtatctc acgaaaacac tcgtgtaaac
     1381 taagataaaa taaatataac aggagaaatt atcatgtcag tagaagaaaa attaaatcaa
     1441 gctaaaggtt ctattaaaga aggtgttggg aaagccatcg gtgatgaaaa aatggaaaaa
     1501 gaaggagcag ctgaaaaagt tgtttctaaa gtaaaagaag ttgccgaaga cgctaaagac
     1561 gctgtagaag gtgctgtaga aggtgttaaa aacatgttga gtggcgacga taaataaggt
     1621 taaaagttac tttatctttt tggtaacatt agtcaaaaga gtctgagtca agatgattct
     1681 cagaaaacaa aaagctagag attcccaatt gcggaactct agctttttaa ttttgcctct
     1741 ttctcttatt atatttcagc aggttgttgg ccatgagtat gaatcccatg tcaattctca
     1801 cttgacgctt acctctcaga tgacatctct tataacccaa acaaaccttt atctgcccaa
     1861 agacagattt catatcaatc ttacgtttag cgaaaatttg tctacccttg gaagataaaa
     1921 gtgcctgata ttctttagtt tttaaacact ggtaacgttc attcatatac agtccctttt
     1981 gaggggctga ttcaggttca taatcgcagt caacattgat ttcaaggctg tttgctttct
     2041 atctcccctt tttcatgata tattttcgta caggtgcttc aatagtttct atagtaaaat
     2101 gaaccaaaaa tagtacacgt tgcggtataa tgttcttgtg gcatattcat tagattttcg
     2161 taaaaagttc tctcctattt gtgagcgaac aggtatagtc aattgattgt cagtttgaag
     2221 accaagagtc gtaaggcaaa ggatatagca gaatccatcc aagggtggct ggcacaattt
     2281 ctagtgaacc tctttaaatc tattacgttt gactgtggaa aagagttttc aaaatggaaa
     2341 gatatttcca atcatcacga tagtgagagc ttttttgcga atctaggatg tccaagacag
     2401 cggtgtctca acgaacattc aaatagactt ttaaggtgtc atgacttgcc aaagcagaca
     2461 gatttcaacg aagtcagtca agaattttag gctgggtaaa aactcaattt caggagaaaa
     2521 tgaaataaat attcccacaa taaaatgcat agtatcaaag tttttccaac acctgatatt
     2581 atgcattttt taattttaaa gactttttcc cagcctcaag aaatcacatc ccacctaaat
     2641 cctttggata ccaaatacct catcaggtat tttctgagtt ggtaagactg tctagcttaa
     2701 tttgacaaat ggagggcgtt aaatccagtt tttacaggaa atctatttaa atattttcac
     2761 actgacctt
//
LOCUS       DAVMEI010000178         2694 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVMEI010000178 DAVMEI010000000
VERSION     DAVMEI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.179"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..757)
                     /gene="lytA"
                     /locus_tag="VYQ24_001842"
     CDS             complement(<1..757)
                     /gene="lytA"
                     /locus_tag="VYQ24_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1968941.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFN"
     gene            complement(1133..2503)
                     /locus_tag="VYQ24_001843"
     CDS             complement(1133..2503)
                     /locus_tag="VYQ24_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1968942.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT          878 a          581 c          516 g          719 t
ORIGIN      
        1 cgttgaaata gtaccactta tcagcgattt tcttccagcc tgtagccatt tcgcctgagt
       61 tgtcgaacca gtaccagttg ccgtctgtgt gcttcctcca gcggtctgca agcatatagc
      121 ctgaactgtc aaagtagtac caagtgccat tgattttctc aaacttgtct tttggataag
      181 agccgtctga atgtacgtac cagtagccag tgtcattctt ctgccagcct gtttcaatcg
      241 tcaagccgtt ctcaatatca tgcttaaact gctcacggct aatgccccat ttagcaagat
      301 atggataagg gtcaacgtgg tctgagtggt tgtttggttg gttattcgtg caatactcgt
      361 gcgttttaat tccagctaaa ctccctgtat caagcgtttt cggcaaacct gcttcatctg
      421 ctagattgcg taagagttcg atataaaggc ggtagtccgt catgaactct tctttggttg
      481 aatggctttc aataagttca accgctgcat aggtctcagc attccaaccg cccccaacgt
      541 cccaggcacc attatcaaca ggtcctacct gcatgatgca accgttccca acaatgtgcg
      601 agaaaaaacc taattctggg tctttccgcc agtgataatc cgcttcattc tgtacggttg
      661 aatgcggatt cccagttgag tgtgcgtgta cttgcctata tggttgcacg ccgacttgag
      721 gcaaatctgt tcttaattta ctcacattaa tttccatatt ctactcctta tcaattaaaa
      781 caactcattt tttacaatcc agaaccagaa actcctttat ttctacctta caaagaagac
      841 aatcttagtc acgattaggc ttgtagatag aacctcaaaa cgcactattt tgacactgta
      901 aataggactg acaaggtctg cattctatct acaataacac cccagactaa aaagcttttc
      961 aaaagtatat ttttacagtc tctatatgtc cttttcataa atactatact ttattatatc
     1021 atataaaaga agtcaaaagt ctgttaaact attttcaaca ccaaactaaa gaagagaaca
     1081 caagtttttt cgatgttcac tagaggaaat ggattttatt cagtaaatcc aactaggatt
     1141 gcactttggt tgccaaaatt gcctttcctt cttttatcaa gggatgacgg aatagtgaga
     1201 agtacagttg aactgtcatg gcaacccaga taagaggttc acaaaggata acacccttat
     1261 atcctgccca aggaataatc aaaaccacaa aaacgatttt tccgattagt tcaataaagc
     1321 tagaaactag aggaaggatc ttctgcccca agccctgcaa gcaattgcga taaatcaaca
     1381 agaggctcaa aatgggataa aaggctgaac tgatttgcag atagagactt ccattttcta
     1441 tcaagtaacc atctgtcgaa ctagccaaga aggaaaccaa ggctggactg gcaaaaaaga
     1501 ggaaaataca aacaaaaact gcccaggata tacttaaacg actgccgatt cgaagacctt
     1561 gaacaatgcg gtcaggtcgc ttagctccta gattctgaga agcaaaggtc gtcattgatg
     1621 cagaaatagc ggtcatagga agaagggcaa aggtcataat gcgtcgagct gccgtctggg
     1681 cactaataat cactgcacca aatgtattaa cagaaaactg taaaatcaca ctaccgatag
     1741 atacaattga actcatcaag cccatagcca aaccttgctc caagagatcc gcgtacaagc
     1801 ttttgtccca tttgaaatgt ttaaactgtg gcaagagttc tggcacactt ttacgaatat
     1861 aataaaagca gagaaccgct gataaacctt gcgaaataat ggtagcaagt cctgcggatt
     1921 gaactcccag atgcaattgc gtaataaaat agagatccag aaccacatta accaaggcag
     1981 agaaaatcag aaatcccagg gctgctagac tgtcaccaat agaccgcaac aagcctgcaa
     2041 aaagattata agcaaagctg acacctacac aggtcacaat catagaaata tattgataag
     2101 attgaggaag aatttctgca ggagtatcta agtattgcaa gagaggatac aagccaagaa
     2161 agcccagcaa cataactaga atgctcaaaa gagcacctaa aatccaggtg gctgctactg
     2221 cttccttgat tttagtgaaa ttccgagccc cataataacg agcaatgaca atccccatgc
     2281 cattgccaac accaagtgta aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta
     2341 ctgcagccaa ggattcttga ccaagaaatc gtccaacaat caagacatca gcagtgttat
     2401 agagctgttg aaaaatattt gatagcaaga ttggaaaggt gaagcttaag agcgagggaa
     2461 gaatcggacc atgtatcagg tccactgttc gttttttatt cataaagcta ttatatcagc
     2521 tttccagagg agcgacaaga aacagcaaac ttatatatag cggaatgaaa taaggacagt
     2581 caaatcgatt tttaacaatg ttttagaagt agatgtgtac tattctagtt tcgatctatt
     2641 atagttggtt gagtttggaa tagtacgctg tagctgctaa aacatttcta gaaa
//
LOCUS       DAVMEI010000179         2681 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVMEI010000179 DAVMEI010000000
VERSION     DAVMEI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.180"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..208)
                     /locus_tag="VYQ24_001844"
     CDS             complement(<1..208)
                     /locus_tag="VYQ24_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1968943.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILI"
     gene            complement(376..1020)
                     /gene="pcp"
                     /locus_tag="VYQ24_001845"
     CDS             complement(376..1020)
                     /gene="pcp"
                     /locus_tag="VYQ24_001845"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1968944.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(1035..1958)
                     /locus_tag="VYQ24_001846"
     CDS             complement(1035..1958)
                     /locus_tag="VYQ24_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1968945.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(1955..2644)
                     /locus_tag="VYQ24_001847"
     CDS             complement(1955..2644)
                     /locus_tag="VYQ24_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1968946.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
BASE COUNT          878 a          591 c          396 g          816 t
ORIGIN      
        1 caatcaaaat ttccacagct ttttccaatt ttttataaaa actatcgaca ttcccattct
       61 ttataatggc aaattgtccg tctaattcgg caatttctcc gataactttt ttaccgatag
      121 tcaatgtata accttcaaaa ctgtctttac caacattaac tttggcatcc gctacttgaa
      181 tttcgatttt tttatctttc ttgctcattt catacctctc ttcacttttt ctattttaca
      241 agaaaatcct aaaactagca agaaaaaact ctatatcagg cttctctgat atagagtttt
      301 atgtaaacaa cctcaatcga tttagcatag ctaatagtta gtaaacaagc ataattgact
      361 atccttttta ggttatcagt gagtctcacc gcctactaac tgcaaatcct tatctttgta
      421 gtctacaata gcttcaatag ctacctctat gcctcttaca atatcacata gactcattcc
      481 tgcagtattc ggcttattta ctacttgttc catcatatat ggaatatgca tgaaacctgc
      541 tctcatatta gggaattttt tatccactaa ataaagagct tggtacatca aatgattgca
      601 aacaaaggtt cctgcactat tggatacaac tgccggaagt ccctgttttt tgatagcttg
      661 taccatcgct ttgataggta aactactaaa ataggccgat gctccatcaa tacgaatcgg
      721 tgtatcaatt ggttgattgc cttcgttatc aggtatgcga gcatcatctt gattaatagc
      781 cactcgttca ggtgttaagc cggtcctgcc gcctgcttgt ccaatacaaa gtacagcatc
      841 tggttgatat cgtaatattt ctgcctctaa aacttctgac gacttataaa aaaccgttgg
      901 aatttctacc cagcgaactt cagccccatt aatctcagat ggtaataatt ttacagcctc
      961 caaagctgga ttaatctttt cacctccaaa aggattaaaa cctgtaacca atattttcat
     1021 tttattttcc tttactaaaa tgcgagaaag tacattaaga atatgtgaat aacaatcatt
     1081 actagagcaa cacctgcttg agcctttata acgccattct gatctttcat atccatcaat
     1141 gctgctggta gagcgttaaa attagcagcc attggggtca ataaggtccc acaataacct
     1201 gctgtcatgg caagagcacc agccacaatt ggattagctc ccagagcaaa tacaaaggga
     1261 actccaacac ctgctgtaat aacggtgaat gctgcaaaag catttcccat aatcattgtg
     1321 aatagaacca ttccaagaac ataggccaaa actcctataa agcgactatc tgaaggaaca
     1381 ataccgctaa tcagatgaga gataacatca ccaacacctg ctacagtaaa aatagccccc
     1441 aaagccccta ataattgagg aacaatacca cttgttgaaa cttgctgagt cattcgatta
     1501 ttttctgata acagactctt agggtgacta ttggtaatca caagaacaga aattgtagca
     1561 aacaaggcgg caaggctaat cgaaatcttg ctaaattctg gaatcatttg cgctaagacc
     1621 aacgcaagta ttgccatcag cataactgga ataaaaattt tatttttcaa cctgttagat
     1681 tcaatattgg ctttcatttc atctaaggat ggcaaggttc cgatacggac ttgcttaaac
     1741 aatgttaaca gcgataatag gattacaata ataccaatac tcatatttgg catataggaa
     1801 ccacctataa acgtaataga caatagagtc caaaatgcag atgtcccaag tcgaactggg
     1861 tttgttttat ctttataact acaataggct gtatggagaa attgacaacc aatcacaata
     1921 taggtcaact ctaatagttg ctttgccaac tctgtcattt ttgttctcct cccctagtct
     1981 tttttgatat caatttttta tcaaataaat aattgtaaat ccccactaca ataagtgtta
     2041 taacagcaac aataatagat gtagaagcaa tccctgcata attgctttca tagcctaact
     2101 gatctaatgt tccccctatc aagaggactc ccccagcacc tacaaacgta ttttgagcaa
     2161 agaaatttcc aaaattttca ttcgcagccg cacgcgcttt tattgtctca tcttcaacct
     2221 ctgttaactt tctacctaat tgagactctg cagctgcttc tcccataggt tgaaccaaag
     2281 gtctgacaaa ctgagggtgt cctcctagac gaattgaaaa gaaaccagct aactctcgaa
     2341 taaaaaaata aactgtatag aagtttccaa ctgtcagacc tttaatcttt cgaatcaaat
     2401 cgattgatcg ttgcttgagt ccaaaggttt ctgacagccc cacaagaggc aaggtaacca
     2461 taaaaatcgt gagcactcgc tgattgctaa attcttttcc caaaatctcc aaaaattcaa
     2521 cgagagaaac acctgaaact aaagctgtaa ccaaaccagc taagactact gttgcaattg
     2581 tatcaaattt taaaataaaa cccacaacaa tgattgctat tcctattaat ctaatccact
     2641 ccatatcaaa ctcctttata ttcaaaatga cagtatttta a
//
LOCUS       DAVMEI010000180         2658 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVMEI010000180 DAVMEI010000000
VERSION     DAVMEI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.181"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..341
                     /locus_tag="VYQ24_001848"
     CDS             <1..341
                     /locus_tag="VYQ24_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1968947.1"
                     /translation="LKYGYTKGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMV
                     GINFGLEFKQGELVISVQEMITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIG
                     VILVVLGILK"
     gene            400..>2658
                     /locus_tag="VYQ24_001849"
     CDS             400..>2658
                     /locus_tag="VYQ24_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1968948.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASIQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELV"
BASE COUNT          906 a          337 c          560 g          855 t
ORIGIN      
        1 gtttgaaata tgggtatact aagggttcta gtcttatcca agaaaataat acaaaaggaa
       61 cattgaatcg cgttacgagt atggcgacag cattagggct agtactagtg ggtggtttga
      121 ttccatcaat ggttggtatt aattttggat tagaatttaa gcagggggaa cttgttattt
      181 ctgttcaaga aatgattaca aaattaattc caggatttat ccctatggct ttgactttat
      241 taatgtgtaa attaattaga aaaggaaaga atccggttgt actaatcttt agtgttatgg
      301 ctattggagt tatcctagtt gttttaggaa ttttgaagta gtagaaagtg tggaggtggt
      361 atttgggata tcacctccat tttggaagag aggtaaagag tgaaattatg gtataagaaa
      421 gctgcctcaa attggaatga agccttgccg attgggaacg gtcatttagg tggtatgatt
      481 tatggttcag ctacaaaaga atgtattcaa ctaaacgatg agactatttg gtatagagga
      541 aagtcagata gaaataatcc agactcacta ttgcatctta aaaaaattcg ggaatatctt
      601 ttagatggag aaattcagaa agccgaagaa ttgataaagt taacaatgtt tgctacccca
      661 agagatcaaa gccactatga attacttggg gaactttaca ttgagcatat agatattcag
      721 tcttgtgctc tttcattgta tgaaagagag ctagatttag atacagctat ttctaatgtt
      781 gtgtttgagc ctaatagttg taatttacaa ataaaaagag aatattttac gagttttaat
      841 aagaatattt tatgttgccg tatagtgtca tcagttcaaa acacattaaa tttaaacatt
      901 aatttgggta gaaataaacg gtttaatgat gaagtatcta aactggattc aagtacaatt
      961 ttaatgtcgg cctctgctgg aggtagaaaa ggtgttcagt ttaaagtggt atgtcattct
     1021 aaggttacgg atggtgaagt aagtgtattg ggagagacaa tagttattcg gaatgctaca
     1081 gaggtatttc tttatctcaa atcaatgacg gattattggg gaaatataga tatttcttct
     1141 cttcagggag aatttagtag tattgattac tttacagaaa aagatgaaca tgtaaaaaaa
     1201 tatcaggagc aatttaatag agttgatttt aaactagact atagtaaaga ttgtcttagc
     1261 attccaacga atctacttct tgaaaacact aaaaagtata gtaactactt gactaacttg
     1321 ttatttcatt atggaagata tctgttaata tcgtctagtc aaccgaatgg tttacctgcc
     1381 aatcttcaag gaatatggtg tgatgaatta aatccaattt ggggttctaa atatacgatt
     1441 aatattaata ctcaaatgaa ttattggatg gtaggtccat gtgatttacc agaagtagaa
     1501 tatccattat ttgatatgct cgaaagaatg agagaaccgg gaagactaac cgctaagaaa
     1561 atgtatggag ctagaggttt tacagcacat cataatacgg atggttttgg cgatacggct
     1621 ccccaatctc atgccatggg ggctgcaatt tgggtattaa ctattccatg gttatgtact
     1681 catatttggg aacactattt atatttccaa gatgagcgta ttcttacgga acattttgaa
     1741 atgataaaag aagcatttct tttctttgaa gattatttat ttgaggtgga tggctacttg
     1801 atgacaggtc caagtgtctc accggaaaat aaatatcgct taaaaaatgg tattgaagga
     1861 aatgcttgtc tatcatctac aattgataat caaattttaa gatatttttg tgattcatgc
     1921 attggaattg caaaacaatt aggagacaat tcggatttta ttagtcgtgt gaaggagtta
     1981 aaaaagaaat tacctagaac aaaaataggt agtaatgggc aaatacaaga atggctagag
     2041 gattatgaag aagtagagcc tggtcataga cacatttcac ctctatttgg gctttatcct
     2101 tataatgaga ttgatattca taaaactccg gaattagcag aagcagctaa aatcactatc
     2161 aataggagat tatcaaacgc taacttttta tcttcacagg atagggagca agcgattaat
     2221 aattggttag taagtggttt gcatgctagt atacaaacag gttggagtgc tgcatggctg
     2281 attcattttt ttgcgagact atatcaaggt gaacctgctt ataaccagat taatggtttg
     2341 ttaaataatg cgactcttgg caatttattt cttgaccatc caccatttca aattgatggt
     2401 aatttaggtt tggtgagtgg aatttgtgaa ttattagtac agagccatca taattggtta
     2461 tcactaattc cagctttacc ttctgcttgg tcagaaggag aagtgaaagg tttcagagta
     2521 agaggaggat ataaggtatc gtttgcttgg aaaaatgggg atataacatt cctaaaattg
     2581 gaaggaggaa acaaagatca aaaagtaaga gtaagaatat atggcaaaaa tactgatgta
     2641 caaaatattg aattggta
//
LOCUS       DAVMEI010000181         2482 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVMEI010000181 DAVMEI010000000
VERSION     DAVMEI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.182"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..1242
                     /locus_tag="VYQ24_001850"
     CDS             22..1242
                     /locus_tag="VYQ24_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEV1968949.1"
                     /translation="MKKILLSTVALLSLVASLLANNPVSAQESSSQATYSKSSGSWIK
                     SGNRWWYKHSDGSYTTNGWEKINGTWYYFDSEGWMKTGWIKEYGKWYYLDDSGAMKTG
                     WCLVSGSWYYLNSSGVMQTGLQTINGKQYYLAAGGAMQTGWHNIGDDTYFFANSGENQ
                     NINRRALVLGETSTRAVPIADVNAMEKVFNNQNFSEVVRFPDRTKSEIIAKMQELFES
                     SSEGDVNYLYFTCHGGRDGRIYIGSDGLAFSGWELASVLKQYKGKFVVMLDCCHAGTI
                     ISKDNTGEGNEGASTEYFDLDEFVSGFSNMDGNEKSGEMIDSKFLVLCSSRGAEYSSG
                     GSLSLATKYWSLGSGWNPLQNSQAYLAADQNNNRRITLNELYTYSREQVLKQNSNQHI
                     EVYPDNSQFVLFKK"
     gene            1255..2436
                     /locus_tag="VYQ24_001851"
     CDS             1255..2436
                     /locus_tag="VYQ24_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1968950.1"
                     /translation="MKVSKKITLFSLSFAGFVLLTLPQAGKAFELKEDWAFKGGIRYE
                     NGKVSKINNGYEVNIKVLDLPSTSAIEWTVRLNGEKQNTNFLAEERTVSKTEDKGRFL
                     HFYIPYGYRGDIVVEAKSGNEVKTWSTKVVDDVYSDSAKSGYFILDGEQILESSWDSV
                     NESYIATLPTVTSGKTVVAWREKGTLNLIKPGRIARQYNSSGSYVELSPIFETASWLK
                     SNQNWYYQKQGQLVQNSWIKDQGSWYFMDDEGVMFNQTWLHQGGSWYAFKSSGAMISA
                     DWLYDNGSWYYLKDSGSMVTGWLKNGGSWYYLNKSGSMATGWIKDSGTWYYLKNSGSM
                     ATGWVKDSGSWYYLKNSGSMATGWVKDNGKWYYLASSGNMLRNTRTPDGYYVDGSGAW
                     K"
BASE COUNT          822 a          344 c          560 g          756 t
ORIGIN      
        1 aatagaaagg acaataatat catgaaaaaa attttattgt caacagttgc tttattaagc
       61 ttagtagcat ctctgctagc aaataatcca gtttctgctc aagaatcatc ctctcaagca
      121 acttatagta agtcaagtgg aagttggata aaatctggta accgttggtg gtataagcat
      181 tctgatggtt cttatactac aaatggatgg gaaaaaataa atggtacctg gtactatttt
      241 gatagtgaag gttggatgaa gacaggttgg attaaagaat atgggaaatg gtattaccta
      301 gatgatagtg gtgctatgaa aactggatgg tgtttggttt ctggtagctg gtattattta
      361 aatagtagcg gtgtaatgca aacgggacta cagactatca atggaaaaca atattattta
      421 gctgccggcg gtgctatgca gacaggttgg cacaatatcg gagatgatac ctatttcttt
      481 gctaatagtg gggaaaatca aaatatcaat cgtcgtgctt tagttcttgg tgaaacatca
      541 acgagagcag tacctattgc agatgttaat gctatggaaa aggtttttaa taaccaaaac
      601 tttagtgaag ttgttcgttt cccagataga acaaaatccg agataattgc aaaaatgcaa
      661 gagctttttg aatcttctag cgaaggcgat gtgaactatc tttactttac ttgtcatgga
      721 ggaagagatg gaagaatcta tattggtagt gatggcctag cattttctgg ttgggaattg
      781 gcgtccgttt tgaaacaata caaaggtaag tttgttgtta tgctggattg ttgtcatgct
      841 ggaacaatta tttctaaaga taatacaggt gaaggcaatg aaggagcctc tacggaatat
      901 tttgatttag atgaatttgt atcaggattt tctaatatgg atggtaacga aaaatcaggt
      961 gagatgattg actctaaatt tttagtatta tgctcatcaa gaggtgcaga atactctagt
     1021 ggtggatcat taagtttagc gactaaatat tggtctttag gttcaggatg gaatcctcta
     1081 cagaattcac aagcatattt agcagcagac caaaataata atcgcagaat cacattaaat
     1141 gaattgtata catactctcg tgaacaagtt ttgaaacaaa attctaacca gcatattgaa
     1201 gtttatcctg acaacagtca atttgtgtta tttaagaaat aagggggcaa taaaatgaaa
     1261 gtatcaaaaa aaattacact atttagtttg tcttttgcag gttttgtttt attgacttta
     1321 cctcaagcag gaaaggcttt tgaacttaaa gaagactggg catttaaagg tggcattcga
     1381 tacgagaatg ggaaagtcag caaaattaat aatggatatg aagtaaatat taaagtgtta
     1441 gatttaccta gtactagcgc aatcgaatgg acagttagat tgaatggaga aaagcaaaat
     1501 actaacttct tagcggagga aagaactgta tctaaaactg aagataaggg acgtttcttg
     1561 cacttttata tcccctatgg atatcgtggg gatattgtag tagaggctaa gagtggaaac
     1621 gaagtgaaga cttggtctac taaggtagtt gacgatgttt attcagattc tgctaagagt
     1681 ggctacttta ttctcgatgg ggaacaaatc ttagaaagtt catgggattc cgtaaatgag
     1741 tcttatattg caacgcttcc aactgtaaca tcaggaaaaa ctgttgttgc ttggcgtgaa
     1801 aaaggaactc ttaatttaat taaaccagga agaattgctc ggcaatataa tagcagtgga
     1861 tcatatgtag aactttctcc aatctttgaa actgctagtt ggctaaaatc aaaccaaaac
     1921 tggtactatc aaaaacaagg tcaattagtt caaaattctt ggatcaagga tcagggaagc
     1981 tggtacttta tggatgatga aggggttatg tttaatcaaa catggctaca ccaaggtgga
     2041 agctggtatg cctttaagtc atcaggagcc atgatttcag ctgattggtt gtatgataat
     2101 ggttcttggt attacctaaa ggactcaggt tctatggtaa caggatggct taaaaatgga
     2161 ggctcttggt attacctaaa caagtcaggt tcaatggcta caggttggat taaagatagt
     2221 ggaacttggt attatctaaa gaattcaggc tctatggcga caggctgggt gaaagatagt
     2281 ggctcttggt attatctaaa gaactcaggt tcaatggcaa caggttgggt caaagataat
     2341 ggtaagtggt actatctcgc atcatctggt aatatgcttc gcaatacacg cacaccagat
     2401 ggctattatg tagatggcag tggcgcttgg aaataaattt ttaaaagaga aaatctactg
     2461 agaaggtaga ttttcttttt ag
//
LOCUS       DAVMEI010000182         2442 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVMEI010000182 DAVMEI010000000
VERSION     DAVMEI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.183"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(129..701)
                     /locus_tag="VYQ24_001852"
     CDS             complement(129..701)
                     /locus_tag="VYQ24_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1968951.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(682..1653)
                     /gene="ruvB"
                     /locus_tag="VYQ24_001853"
     CDS             complement(682..1653)
                     /gene="ruvB"
                     /locus_tag="VYQ24_001853"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1968952.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHAGLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1814..1906
                     /locus_tag="VYQ24_001854"
     CDS             1814..1906
                     /locus_tag="VYQ24_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1968953.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT          758 a          557 c          431 g          696 t
ORIGIN      
        1 aaaaaataag ccgcctgatt gggcgacttt atttttatag ggagattatt atgaaaaagt
       61 tttaggagtt taagttaagg tcttcttaac ttatgaactt agtgtacact ccctagctta
      121 aagtttcctt aagtattttt aaaaatcaaa tttttccatt tctcctgcca atttttcttg
      181 gataaacgtg tttgatagag ttccattcgg tcttcatttt ctaagaaatg aggagttgga
      241 cgaacttgaa aattcaaaat atcctccaaa ccataaggtg catagagttc aaaatctgat
      301 tcttcattca agcgcagtcc aactgtcgta caccgttctg gatacttact catagcatca
      361 cgagaactgg tataggaagc agtgtgagga ctgtgctgat gcatatagac ctgatttttc
      421 aattcccact ggtactgagg aaaatcctct cgcagctttt tctccagtaa taaggtttcc
      481 tcataagaaa aatctggatc aaagaaaatc acatctatat ctgtttcatg atcaaaaggg
      541 gatttgtctg acaaaagatt ccaaatgaaa tttctgacag aacctgctgc caaccacgag
      601 tctttcagac caaggtctcg gatgatcgtc agaatggcca tcatatctgg attttctcta
      661 aaagcctcta agatttcttg cttatttttc actgtattca taacctaagt gctcatatgc
      721 cttagcagtc gccacccgtc cagaccgtgt ccgcatgata aaaccttttt gaatcaagta
      781 aggctcatac atatcttcaa ctgtctcacg ctcttcggcg atattcacag aaagagttcc
      841 tagaccaaca ggtcctccac tgtacatctc aatcatggta cgaaggattt tttgatccac
      901 atagtccaaa ccttcatggt caacatccag catagtcaaa gccttatcgg taataatatc
      961 atcaattacc ccattcccca ttatctgggc aaaatcgcgc acgcgcttga ggagacgatt
     1021 ggcaatacga ggggtcccac gactacgtag ggccaactca gatgctgcct catgagtgat
     1081 ttccatctca aaaatatctg ccgtccgctc gacaatttct gtcaagccag catgggcata
     1141 atactccata tggcctgtaa tcccaaaacg tgcccgtagc ggattggaga gcataccagc
     1201 ccgagtcgtc gcaccaatca aggtaaaagg tggtaactcc aaatgaacac tacgactacc
     1261 ctcaccagcc ccaatcataa tatcgatgta gaagtcctcc atagcactat aaagcacctc
     1321 ttccactgac attggcaaac gatggatctc atcaataaaa agtacatccc caggctctaa
     1381 ctcattcaaa atagctacca gatctccggc tttttcaatg actggacccg acgtctgctt
     1441 aagattgact cccagttcgt tggcaataac aaaggccatg gtcgttttcc cgagacctgg
     1501 aggcccaaat aagagcacat gatccagcgc ttcatcccgc attttggcag cttcaataaa
     1561 gatttgtagc tggtccttga ccttatcctg tccgatatat tcacgtaaat actgaggacg
     1621 gagcgtgcgt tctactaact cctcatcccc cattatctca ttatctaaaa ttctactcat
     1681 ggctctatta tatcaaaaaa ccaagccaca aacaaaaaag ccacctgatt gggtgactcc
     1741 taagtttagc acttatgtgg tataatattc tacgacactg ctacaccgcc tacgaaagga
     1801 ggtgagatag cccatgatgg aattagtact caaaactatc atcggaccaa ttgtggtcgg
     1861 tgtcgttctt cgtatagtcg ataaatggct aaacaaggac aaatagtgtc aaaaaagacc
     1921 tcaagcttat ttggtcgtga gcttggggtc ttttctagcc tatgatatag aactagtact
     1981 caattccttt ttattatccc atagttcacg aattttgtca aaggtttaca ttttcttcaa
     2041 ccgctgtacg acaagacggt taagattaag agaacgttaa ggattctatc aatttcatag
     2101 aaattttgat ttcgtaaacg aagagacaat cttacatgtt acttctcatt taatacgcca
     2161 ctactagaca agcaaaatca ttattacagt agttccagtt cttcaattaa cagtcactta
     2221 caatcaaatt gagtttgaac tagctgaagc gaccacagac ctatttctta gttatattcg
     2281 ctaaaaaagt ccccgccaat tccccgacca aaatccgaaa aataccgaaa aatatcgaaa
     2341 aattattttt agaatagtcc ccaaaagcct gaaatagagc taaaaaactc cacctgattg
     2401 ggtgaagtta agggagatta ttatgaaaaa gaaaagttta gg
//
LOCUS       DAVMEI010000183         2435 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVMEI010000183 DAVMEI010000000
VERSION     DAVMEI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.184"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(69..1235)
                     /locus_tag="VYQ24_001855"
     CDS             complement(69..1235)
                     /locus_tag="VYQ24_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1968954.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            1293..>2435
                     /locus_tag="VYQ24_001856"
     CDS             1293..>2435
                     /locus_tag="VYQ24_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1968955.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQIN"
BASE COUNT          801 a          377 c          558 g          699 t
ORIGIN      
        1 agaaagccct gtcaagagaa atgtgtaaaa tttttatata taaaaaactt ctagctaaaa
       61 ctagaagttt aaaggatctt atccgctctg tccactgtaa agagggccac agtcatcagg
      121 atatagatga gcaagagggc agctacagct ggtacccaag agtggaacag gtcaaaactg
      181 taaccaaaga gggttggccc aaaggctgct aggatatagc ctcctgtttg agataggccg
      241 gacaattggg ctgtcttttc aggggcgctt gtcttgagtg aaaagttgac catgagataa
      301 gggaagaggg cgctggttgc ggttccgatg aggagatgga tggtaagcca gtaaatgaaa
      361 ttattgattg ggaaaaagag catggaaatg ccgaccacac cagctagtga aaccagagtg
      421 agcatgagct gacggttgcg agtagataaa ctggttgtca ggcttgggat ggtcattgaa
      481 aaaggaatgc taatcagaga taagatagaa gtcagcaagc cagcttcgtg actggataga
      541 cctgcatgga tagacatggt aggtaaccag gtcatgacgg tgtaaaagat caaggattga
      601 aaacctgaaa agataataat tgcccaaacc tgtttattac gcatgacctt tatttgactt
      661 ttttgtttgg tttgtggagc tagtctatga ttatagcggt gatttgggag ccagaccaaa
      721 aaagttgcta gacagagtaa cgtgaggaga aggataagtc ctttccaaga actggcttgt
      781 gtaatgggca cagctagata ggaagccaga gccgttgcaa tccccataga cgttacatat
      841 aaggtggtca gaaaaccaat tttctttggt tgattggctt ggataagact aggaagcagg
      901 acattgatga ctgcgacact tgccccaacc atcaaggttc ctagatagag caggggcaga
      961 ttgattagtc gaataagtga gccgatggtc aagaagaaga ggctgtaggt gaagagatgc
     1021 tccaagccga ttttctgagc cagttgggta gaaaatggtg aaaagagggt aaacataagg
     1081 agaggcaggc tggtcaagac accaagcgaa ctaacttcta cctccagacc ttgcgaaata
     1141 tttcccaaaa taatgggtaa aacagtaaaa ggagttcgca aggaaacacc aatcaggata
     1201 atacctggaa caaaaaagag tgattgcttt ttcatataat acctcttcta gctgatttta
     1261 ataacagctt attttaaggc tttttcacta taatgtcaag taaggtttct ggctttcaag
     1321 ataaaaatgg gaaagtcttg aaaattatga tagaatggtg gaaggaaaaa ttcaggagag
     1381 tagtagtgac tcaaaatgtt gaaagtcttc tcgtatccat tgtaatcagt gcatacaatg
     1441 aagaaaaata tctgcctggt ctaattgaag acttaaaaaa tcaaacctat cctaaagaga
     1501 atattgaaat tctatttata aatgctatgt ccacagatgg gaccacagct atcattcagc
     1561 aatttataaa ggaagataca gagtttaact caattagatt gtataacaat cctaagaaaa
     1621 atcaagctag tggttttaac ctgggagtta aacattctgt aggggacctt attttaaaaa
     1681 ttgatgctca ttcaaaagtt actgagactt ttgtaatgaa caatgtggct attattcaac
     1741 aaggtgaatt tgtctgtggg gggcctagac cgacgattgt cgaaggaaaa ggaaaatggg
     1801 cagagacctt gcatcttgtt gaggaaaata tgtttggcag tagcattgcc aattatcgaa
     1861 atagttctga ggatagatat gtttcttcta tttttcatgg aatgtataaa cgagaggttt
     1921 tccagaaggt tggtttagta aatgagcaac ttggccgaac tgaagataat gatattcatt
     1981 atagaattcg agaatatggt tataaaatcc gctatagccc aagtattcta tcttatcagt
     2041 atattcgacc aacattcaag aaaatgctgc atcaaaagta ttcaaatggt ttgtggattg
     2101 gcttgacaag tcatgttcag tttaagtgtt tatcattatt tcactatgtt ccttgtttat
     2161 ttgttttgag tcttgtgttt agtctagcat tgttaccgat cacattcgta ttcataactt
     2221 tactattagg tgcctatttt ctacttttgt cattactcac tttgctgact ttattaaaac
     2281 ataaaaatgg atttctaatt gtgatgccct ttcttttatt ttccattcac tttgcttatg
     2341 gccttgggac gattgtaggt ttaattagag gatttaaatg gaagaaggag tacaagagaa
     2401 caataattta tttggataaa ataagccaaa taaat
//
LOCUS       DAVMEI010000184         2433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVMEI010000184 DAVMEI010000000
VERSION     DAVMEI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.185"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..1675
                     /gene="mltG"
                     /locus_tag="VYQ24_001857"
     CDS             20..1675
                     /gene="mltG"
                     /locus_tag="VYQ24_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1968956.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPAKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAETF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            1746..2228
                     /gene="greA"
                     /locus_tag="VYQ24_001858"
     CDS             1746..2228
                     /gene="greA"
                     /locus_tag="VYQ24_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1968957.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(2285..>2433)
                     /locus_tag="VYQ24_001859"
     CDS             complement(2285..>2433)
                     /locus_tag="VYQ24_001859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968958.1"
                     /translation="SKHGFEVVDETDEVSSKHCFEVVDETDKVSNHIYGKATLTWFEE
                     IFEE"
BASE COUNT          820 a          461 c          530 g          622 t
ORIGIN      
        1 caacaaggag gaaaaacttt tgagtgaaaa gtcaagagaa gaagagaaat taagctttaa
       61 agagcagatt ctgagagatt tagaaaaagt aaaaggctat gatgaagttc tgaaagaaga
      121 tgaggcagta gttcgcactc ctgcaaatga accttcagct gaagaactca tggctgattc
      181 cttgtcaacg gtagaggaga ttatgagaaa agctcctacc gtgcctactc acccaagtca
      241 aggtgtacca gcttctccag cagatgagat tcaaagagaa actcctggtg ttccaagtca
      301 tccaagtcaa gatgtacctt cttctccagc ggaagaaagt ggatcaagac caggtccagg
      361 tcctgttaga cctaagaaac ttgaaagaga atacaatgaa accccaacaa gggtagctgt
      421 ttcctatacg acggcagaga aaaaagcaga acaagcaggt ccagaaacac ctacgcctgc
      481 tacagaaaca gtggatatca tcagagatac atcacgtcgt agccgtagag aaggagcaaa
      541 acccgctaag cctaagaaag agaagaagtc acatgtgaaa gcttttgtga tttcattcct
      601 tgtattcctt gccttgctct cagcaggtgg ttactttggt taccagtacg tgctagattc
      661 cttattacct atcgatgcta attctaagaa atatgtgacg gttggaattc cagaaggttc
      721 aaacgttcaa gaaatcggta cgacgcttga aaaagctggt ttggtaaagc atggtctgat
      781 ttttagtttt tatgccaagt ataaaaatta taccgacttg aaagcaggtt actacaattt
      841 gcaaaagagt atgagtacag aagacttact caaagagttg caaaaaggtg gaacagatga
      901 accgcaagaa cctgtacttg cgactttgac aattccagaa ggttatacct tggatcagat
      961 tgctcaagct gtgggtcaat tgcaaggtga cttcaaagag tctttgacag cggagacttt
     1021 cttggctaaa gttcaagatg agacgtttat cagtcaagca gtagcgaaat atcctacttt
     1081 actggaaagt ttgcctgtaa aagacagcgg tgcgcgttat cgtttggaag gatacctttt
     1141 cccagctaca tactctatca aggaaagcac aactattgag agcttgattg atgagatgtt
     1201 agctgctatg gataagaacc tatctcctta ctatagtact atcaaatcta aaaacttgac
     1261 tgtcaatgag ttgttgacca ttgcttcctt ggtcgaaaaa gaaggtgcca agacagaaga
     1321 tcgtaagctc attgcaggtg tattctacaa tcgtttgaat cgtgatatgc cacttcaaag
     1381 taatattgca atcttgtatg cccaaggaaa actggggcaa aatatcagtc tagctgagga
     1441 tgttgcgatt gataccaaca ttgattcacc ttataatgtt tataaaaatg taggtctcat
     1501 gcctggtcca gtcgatagtc caagtctgga tgcgattgag tcaagcatca atcaaactaa
     1561 gagcgataac ctctactttg tagcagatgt cacagaaggc aaggtctact atgctaacaa
     1621 tcaagaagac cacgaccgca atgtcgctga acatgtaaac agcaaattaa actaaacaaa
     1681 ctaaaattat gtgatatttc acataatttt ctttagaaaa tattatcaga agaaagttga
     1741 gaaaaatggc agaaaaaaca tatcctatga cccttgagga aaaggagaaa cttgaaaaag
     1801 aattagaaga attgaaattg gttcgtcgac cagaagtggt agaacgcatt aagattgccc
     1861 gttcatacgg tgatctttca gaaaacagtg agtacgaagc agctaaggat gaacaagcct
     1921 ttgtcgaagg acaaatctct agcttagaaa caaaaatccg ctatgctgaa atcgtcaata
     1981 gcgacgcagt tgcccaggac gaagtagcga ttggtaaaac agtcaccatc caagaaattg
     2041 gcgaggacga agaagaagtt tatattatcg taggttcagc tggtgcggat gcctttgcag
     2101 gtaaggtttc aaatgaaagc ccaattgggc aggccttgat tggcaagaaa acaggtgaca
     2161 cagcaacaat tgaaacgcct gttggtagct atgatgtaaa aatcttgaag gttgaaaaaa
     2221 cagcctaaaa acagaaaaag gagtggggag gcgatgtgct tcactcactc ctttttccat
     2281 tttgctattc ttcgaaaatc tcttcaaacc acgtcagcgt cgccttgccg tatatatggt
     2341 tactgacttt gtcagtttca tctacaacct caaaacagtg ttttgagcta acttcgtcag
     2401 tttcatctac aacctcaaaa ccatgttttg agc
//
LOCUS       DAVMEI010000185         2365 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVMEI010000185 DAVMEI010000000
VERSION     DAVMEI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.186"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..229
                     /locus_tag="VYQ24_001860"
     CDS             <1..229
                     /locus_tag="VYQ24_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968959.1"
                     /translation="VYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSETLEKG
                     PESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            310..1131
                     /gene="cbpG"
                     /locus_tag="VYQ24_001861"
     CDS             310..1131
                     /gene="cbpG"
                     /locus_tag="VYQ24_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HEV1968960.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVNTLEGSSGSTVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGNWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            1150..2172
                     /gene="cbpF"
                     /locus_tag="VYQ24_001862"
                     /pseudo
     CDS             1150..2172
                     /gene="cbpF"
                     /locus_tag="VYQ24_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
BASE COUNT          770 a          343 c          532 g          720 t
ORIGIN      
        1 tgtttataaa aaattgggta tttcaattat tgcgagtact ttattggcta gccagttatc
       61 gacagtatct gctttgagtg ttatttctag tacaggtgaa gaatatgagg taagtgagac
      121 actagaaaaa ggtccagagt ctaatgattc ttcattatct gagatttcac caacgtatgg
      181 ttcatactac caaaagcaat cagaagtatt atcggtaatg atgatttgat taaaattgat
      241 aatactttac agtatccgta ttcgacttca gctatggttt tatctaagta ttatggagta
      301 gccgatggaa tgaatgtaga agggagggga agtgcgaatt ttattaaaga taatgtgtta
      361 attacagcgg ctcacaacta ctacagacat gactatggga aagaagcgga tgatatttat
      421 gttcttccgg ctgttagtcc aagtcaagaa ctatttggaa agatcaaagt aaaggaagtt
      481 cgttatttga aggaatttag aaatttaaat tctaaggatg caagggaata tgacttggct
      541 ttattaattc tagaagagcc cattggtgca aaattaggga ctttgggtct tcctactagt
      601 caaaaaaatt tgacaggaat aactgtgact atcacaggct atctatcata taattttaaa
      661 attcatcaaa tgtatacaga taaaaaacaa gttttaagtg atgatggcat gttcttggat
      721 taccaagtta atactttaga ggggtctagt ggatctacag tttatgatgc tagtcaccgt
      781 gtagtaggag tgcatacttt aggagatgga gctaatcaaa ttaacagtgc agttaaatta
      841 aatgaacgaa atttgccatt tatttattcg gttcttaaag gttactctct tgaaggatgg
      901 aagaaaataa atggtagttg gtactattat agacaacatg ataaacaaac gggttggcag
      961 gagataaatg atacttggta ttatttagac agttccggta agatgcttac agattggcaa
     1021 aaagtaaatg gaaactggta ttatctcaat tcaaatggag caatggttac aggtagccaa
     1081 actatcgatg gtaaagttta taacttcgct tcatctggtg agtggatttg atgttggagg
     1141 atatataaaa tgaagctttt gaaaaaaatg atgcaagtcg cactagccac atttttcttc
     1201 ggtttgctag ggaccagtac agtatttgca gatgattctg aaggatggca gtttgtcaaa
     1261 gaaaatggta gaacctacta caaaaagggg gctctaaaag aaacctactg gagagtgata
     1321 gatgggaagt actattattt tgatccttta tccggagaga tggttgtcgg ctggcaatat
     1381 atacctgctc cacacaaggg ggttacgatt ggtccttctc caagaataga gattgctctt
     1441 agaccagatt ggttttattt tggtcaagat ggtgtcttac aagaatttgt tggcaagcaa
     1501 gttttagaag caaaaactgc tacgaatacc aacaaacatc atggggaaga atatgatagc
     1561 caagcagaga aacgagtcta ttattttgaa gatcagcgta gttatcatac tttaaaaact
     1621 ggttggattt atgaagaggg tcattggtat tatttacaga aggatggtgg ctttgattct
     1681 cgcatcaaca gattgacggt tggagagcta gcacgtggtt gggttaagga ttaccctctt
     1741 acgtatgatg aagagaagct aaaagcagct ccatggtact atctagatcc agcaactggc
     1801 attatgcaaa caggttggca atatctaggt aataagtggt actacctcca ttcgtcagga
     1861 gctatggcaa ctggctggta taaggaaggc tcaacttggt actatctaga tgctgaaaat
     1921 ggtgatatga gaactggctg gcaaaacctt gggaacaaat ggtactatct ccgttcatca
     1981 ggagctatgg caactggttg gtatcaggaa ggttcgactt gatactatct aaatgcaagt
     2041 aatggagata tgaaaacagg ctggttccaa gtcaatggta actggtacta tgcctatgat
     2101 tcaggtgctt tagctgttaa taccacagta ggtggttact acttaaacta taatggtgaa
     2161 tgggttaagt aatgaaggct aattgtaaac tgtgatggat acttaacttt gtataatagg
     2221 tggataaaag tcttcacaat caaaaaacgc atagtatcaa ggtttttctg tactgccccc
     2281 caaaagttag acaaataatt tatccaaagg atttagttct gtactgcaca ggactaagtc
     2341 cttttagttt taccttaatt ctctt
//
LOCUS       DAVMEI010000186         2359 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVMEI010000186 DAVMEI010000000
VERSION     DAVMEI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.187"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            142..954
                     /locus_tag="VYQ24_001863"
     CDS             142..954
                     /locus_tag="VYQ24_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1968961.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKETIKKVAEKGHQIIITTG
                     RPYRMSKDFYHELGLNTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVRRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPENQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            1293..2312
                     /gene="adhP"
                     /locus_tag="VYQ24_001864"
     CDS             1293..2312
                     /gene="adhP"
                     /locus_tag="VYQ24_001864"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1968962.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKGKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT          693 a          491 c          493 g          682 t
ORIGIN      
        1 tatcattcac tttttcaata tctttgtttg atttatttaa gaatttgatg aaatttcttt
       61 ttttgtttcg aatctgcttt tctgcctgct ttctgttata atagtagaca tcttatctga
      121 aaagacacta gaaaggtccc tatgacgaaa aaaattattg cagttgacct ggatggaacc
      181 ctgctcaact cagacagtca aatttctgac tttaccaaag aaaccattaa aaaagttgct
      241 gaaaaaggcc atcagattat tattacgacg ggtcgccctt accgcatgtc aaaagatttt
      301 taccatgaac taggcttaaa cactcctatg attaatttca acggctccct tactcattta
      361 ccagaccaag tttgggattt tgaaaagtgt ttaactgtag acaaaaaata tctgctagac
      421 atggttcgac gttcagagga cattcaagcc gattttatcg ctggagaata ccgtaaaaaa
      481 ttttacatta caaatcccaa tgaagaaatt gctaatccca aactatttgg tgtagaagct
      541 ttccagcctg aaaatcaatt tcagcctgag ttagtaacca aggatcctaa ctgtattctg
      601 ttacagacta gagccagtga caaatatgct ctggcaaaag aaatgaacgc cttctaccag
      661 catcaactgt ctatcaatac ctggggaggt ccgctcaata tccttgaatg taccccaaaa
      721 ggtgtcaaca aggcctttgc tttggactac ttgctcaaga taatgaatcg tgacaaaaaa
      781 gatttgattg cctttggaga tgaacacaat gataccgaaa tgctcgcttt tgctgggaag
      841 ggttatgcca tgaaaaatgc caatccagag ctactccctt atgcagatga gcaaatttcc
      901 cttaccaacg accaagatgg ggttgccaaa accctacaag acttattctt ataacctata
      961 ctgatactca atgagaatca aagagcaaac taggaagcta gccgtaggtt gctcaaagca
     1021 ctgctttgag gttgtagatg aagctgacgt ggtttgaaga gattttcgaa gagtatgacc
     1081 agcttgcaag ctggtctttg tgctcttttc acagtctcat caaccagtta atcgattgtt
     1141 ctactttttg tctccaaaac cttggaaaag gctttctttt gtgatatact tcacatataa
     1201 atacaagaga gctcgtcaca ctcgactctg ttgacaaaaa atcaatccag tcccgttgtc
     1261 cctgttctcg gtcaatatca aggaggatag ctatgaaagc tgttgttgta aatccagaaa
     1321 gcactggtgt tgctattgaa gaaaaagtac tccgtccact tgaaactggg gaagcacttg
     1381 tagaagttga atactgtggc gtttgccaca ccgatctcca cgttgctcat ggtgactttg
     1441 gtcaagtccc aggacgtgtt cttgggcacg aaggtatcgg tatcgttaaa gagattgctc
     1501 cagatgtgaa aagccttaaa gtcggtgacc gcgtcagcgt tgcttggttc tttgaaggat
     1561 gtggcacttg cgaatactgt acaactggtc gcgaaaccct ttgccgtaca gtgaaaaatg
     1621 ctggctactc agtagacggt ggtatggctg aacaatgtat cgtaactgct gactatgctg
     1681 tcaaggttcc tgacggactt gatccagccc aagcttcttc tatcacatgt gctggagtaa
     1741 caacctataa agctatcaaa gaagcaaaag ttgaaccagg ccaatgggtt gttctttacg
     1801 gtgctggtgg acttggtaac ctcgctgttc aatacgctaa aaaagtattc aatgctcatg
     1861 ttatcgcagt cgatatcaac aatgacaaac ttgcccttgc aaaagaagta ggcgctgaca
     1921 ttgtgattaa cggcctcgaa gttgaagatg tagctggact cattaaagga aaaactgatg
     1981 gaggagctca ttcagctgtc gtaactgctg tgtctaaagt tgccttcaac caggctgttg
     2041 actccgttcg tgctggtggt cgcgtcgtcg ctgttggtct tccttctgaa atgatggaac
     2101 tcagtatcgt taaaacagtc ctcgatggaa tccaagtcat cggttctctt gtcggaactc
     2161 gtaaagactt agaagaagcc ttccaatttg gtgcagaagg tctggtagcc ccagttgttc
     2221 aaaaacgtcc agtagaagat gctgttgcca ttttcgacga aatggaaaaa ggtcaaatcc
     2281 aaggacgtat ggtactcgac ttcacccact aatctaatag aaacctattt taaaagttgg
     2341 aagcttcttc taacttttt
//
LOCUS       DAVMEI010000187         2357 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVMEI010000187 DAVMEI010000000
VERSION     DAVMEI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.188"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..2251
                     /locus_tag="VYQ24_001865"
     CDS             <1..2251
                     /locus_tag="VYQ24_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1968963.1"
                     /translation="ENKDLKKLIKKKFREVEDFVTGGKRTVELDYKYDDKGNITAYDD
                     GSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEIKA
                     TKYDSHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDDDRIKAETKIRMPEKNKETKA
                     EYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYAL
                     KVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQSV
                     LMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNV
                     EAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELR
                     VNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNV
                     YLQTGYSNLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNG
                     FDGEIQEQHIYINGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEV
                     SELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAG
                     YVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQRE
                     DHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIF
                     LGLKKKNQD"
BASE COUNT          969 a          286 c          445 g          657 t
ORIGIN      
        1 tgaaaataaa gacttaaaga aactcattaa aaagaaattt agagaagttg aggactttgt
       61 aactggtgga aaaagaacag tagaacttga ttataaatat gacgataaag gaaatataac
      121 tgcctatgac gatggtagtg ccttacaata tgaaactgaa aaacttgacg aaataaaatc
      181 aaaaatttat ggtgttctaa gcccatctaa agatggacac tttgaaattc ttggaaagat
      241 aagcaatgtt tctaaaaatg ccaaggtata ttatggaaat agctataaat cgatagaaat
      301 caaagcgact aagtatgact cacattcaaa aacgatgaca tttgatttat acgctaatat
      361 taatgatatt gtggatggat tagcttttgc tggagatatg agattatttg ttaaagatga
      421 tgatcgaata aaagctgaaa ctaaaattag aatgcctgaa aaaaataagg aaactaaagc
      481 agaatatccg tatgtatcaa gttatgggaa tgtaatcgaa ttaggagaag gagatctttc
      541 aaagaacaaa ccagacaatt taactaaaat ggaatctggt aaaatctatt ctgattcaga
      601 aaaacaacaa tatctgttaa aggataacat cattctaaga aaaggctatg cactaaaagt
      661 gactacctat aatcctggaa aaacggatat gttagaagga aatggagtct atagcaagga
      721 agatatagca aaaatacaaa aggccaatcc taatctaaga gtcctttcag aaacaacaat
      781 ttatgctgat agtagaaatg ttgaagatgg aagaagtacc caatctgtat taatgtcggc
      841 tttggacggc tttaacatta taaggtatca agtgtttaca tttaaaatga acgataaagg
      901 ggaagctatc gataaagacg gaaatcttgt gacagattct tctaaacttg tattatttgg
      961 taaggatgat aaagaataca ctggagagga taagtccaat gtagaagcta taaaagaaga
     1021 tggctccatg ttatttattg ataccaaacc agtaaacctt tcaatggata agaactactt
     1081 taatccatct aaatctaata aaatttatgt acgaaatcca gaattttatt taagaggtaa
     1141 gatttctgat aagggtggtt ttaactggga attgagagtt aatgaatcgg ttgtagataa
     1201 ttatttaatc tacggagatt tacacattga taacactaga gattttaata ttaagttgaa
     1261 tgttaaagac ggtgacatca tggactgggg aatgaaagac tataaagcaa acggatttcc
     1321 agataaggta acagatatgg atggaaatgt ttatcttcaa actggctata gcaatttgaa
     1381 tgctaaagca gttggagtcc actatcagtt tttatatgat aatgttaaac ccgaagtaaa
     1441 cattgatcct aagggaaata ctagtatcga atatgctgat ggaaaatctg tagtctttaa
     1501 catcaatgat aaaagaaata atggattcga tggtgagatt caagaacaac atatttatat
     1561 aaatggaaaa gaatatacat catttgatga tattaaacaa ataacagaca agacactaaa
     1621 cattaagatt gttgtaaaag attttgcaag aaatacaacc gtaaaagaat tcattttaaa
     1681 taaagatacg ggagaggtaa gtgaattaaa acctcatagg gtgactgtga ccattcaaaa
     1741 tggaaaagaa atgagttcaa cgatagtgtc ggaagaagat tttattttac ctgtttataa
     1801 gggtgaatta gaaaaaggat accaatttga tggttgggaa atttctggtt tcgaaggtaa
     1861 aaaagacgct ggctatgtta ttaatctatc aaaagatacc tttataaaac ctgtattcaa
     1921 gaaaatagag gagaaaaagg aggaagaaaa taaacctact tttgatgtat cgaaaaagaa
     1981 agataaccca caagtaaacc atagtcaatt aaatgaaagt cacagaaaag aggatttaca
     2041 aagagaagat cattcacaaa aatctgattc aactaaggat gttacagcta cagttcttga
     2101 taaaaacaat atcagtagta aatcaactac taacaatcct aataagttgc caaaaactgg
     2161 aacagcaagc ggagcccaga cactattagc tgccggaata atgtttatag taggaatttt
     2221 tcttggattg aagaaaaaaa atcaagatta agataaaagc tatagaaaaa aatggtttat
     2281 gtactgagat tagatagtga ggtgatgaca tagttttgtg aaaacagcca tttataactc
     2341 aattatttag tttactt
//
LOCUS       DAVMEI010000188         2285 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVMEI010000188 DAVMEI010000000
VERSION     DAVMEI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.190"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..503)
                     /locus_tag="VYQ24_001866"
     CDS             complement(<1..503)
                     /locus_tag="VYQ24_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968964.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRLFSN
                     FDDFLEK"
     gene            complement(517..1230)
                     /locus_tag="VYQ24_001867"
     CDS             complement(517..1230)
                     /locus_tag="VYQ24_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1968965.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1688..2068)
                     /locus_tag="VYQ24_001868"
     CDS             complement(1688..2068)
                     /locus_tag="VYQ24_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1968966.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
BASE COUNT          761 a          440 c          344 g          740 t
ORIGIN      
        1 tttttttcta aaaagtcgtc aaaattagaa aacaatcgaa aatacaatgc attctcttgg
       61 tgtaaaatcg ctctttttaa aggataatac tctttaatat tattaacttt ttgttcgtag
      121 gcatattttg ctacatgatc taggtatata tcaaaaaagt catgaggaaa atctcccata
      181 cagccattac gattcatatt gaggctatat tttcgattag taagataatt cggaaatata
      241 tttcctatta ctctaagatt atctacttgt gaatcccttc tcccaatata atccccttct
      301 ttgactaatg atggagattt ccaagtaaaa attaaatcgc cagagaaatt tttcttatca
      361 aaaaacttaa aaagagattg ataaatacta tttgctaatt gacaagtgtc agatgtaaat
      421 tcataattaa cagcttcatt aaaatcaact tcagacattt caatgtactc ttcaatagta
      481 atattagctt tttttgtagt cattagctca tctcctttaa aatgtgatct tttcaagaat
      541 atttttatct aaacaatcca gcaagtcttg gtaagaatag acttcgtaag tcggctgggc
      601 ttgtgtgtga ttttcgaggt gatgaggatt ataccagata gtgtcaatcc ccgcattatt
      661 gccaccttga atgtcggcgg ttagagaatc tccaatcatc agcgtctttt ctttactaaa
      721 tccagcaatt tgctggccaa tcttttcata aaagagagca tccggctttt gagtttgcaa
      781 ctgttctgag ataaagactt gattgaaata aggtactaga ccagattgag ccaaacgtcc
      841 tgtctgaatg gcagtaatgc catttgtcgc agcatacaag tcataatcac gctcaatgag
      901 gctgtccaag agatcatgag cgcccgatag tgtttgtccc tgctgagcga gataaaattg
      961 gtaacgctgg gcaagaaaac taccgtcttt ttcctgtcca aaatgagaaa ataaacgaga
     1021 aaagcgcgtg ttaaccaact cttgtttact gattttcttc agctccaagt ctttccagag
     1081 agccttgttc ataggaacgt aataatcttt ataagcctga atatccgcaa ctccttcttc
     1141 ttttagaagt tgggtcaaag ccacatcctc agcagcatca aaatcaagaa gagtgtggtc
     1201 gaggtcgaag agtagaaatt tgtagaacaa tttgaggttt tcctttctga aaattcatta
     1261 agaacattat atcataaagc acctcataca attaactaat ttaatcactt aaaaaaattc
     1321 gaacactttc tatacaactg acagctcaaa tctttcagaa tagaacaata ctaactatcg
     1381 aacaccccgt cttcataaat acatatgtaa ttctagacct agaattccta taaactaaat
     1441 gctttcatac tcttccaagt aattgattgc cttaaatttt aatttttgaa ggattctaaa
     1501 gctagaatag ccccatcaca atcagttttg attgattcac aatttagaaa cactatagtt
     1561 tcactcctgt taaaataaaa aggaactgca taaagcaatc cctttctgat tttgaaatca
     1621 tttacttaac attttatagt tgagataatc aatagcttat ctataaaaag agttatagta
     1681 aaattcctta tttattgatt tcaagctccg ctaactgtat ttgaataact gacagttctg
     1741 caccagcctg aagaagagca gctgcattat aggcaccttc tacaattgga accctgttga
     1801 tgatgatact tttatcactg aaatcagtca ccatttttaa gttcatttta gcagaaccta
     1861 ggtcaaaaaa ggcaagtaaa gtatctgctg gattttcgga aacaacccta tctacttgat
     1921 caaaactcgt tccaattcct ccgccctcgg ttcctcctac ataagtaatc ggaacatctt
     1981 tagctacttt actaatcagt tcaacaacac cttctgcaat atgtttggaa tgtgaaacga
     2041 taacaagacc aataccaata ctttccatca aaccactcca gtttctaaaa tagcagtaaa
     2101 gagtaatcct gatgagaatg atccaggatc aatatgtcca agaaaccaca tgctcctaag
     2161 acaagagcta acagactggc catcaataat agtattgttc tttttttcat cattactcct
     2221 taactagtgt ttaactgatt aattagccag taaatagttt atctttattt acactatctg
     2281 ttaag
//
LOCUS       DAVMEI010000189         2275 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVMEI010000189 DAVMEI010000000
VERSION     DAVMEI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.191"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..231
                     /locus_tag="VYQ24_001869"
     CDS             <1..231
                     /locus_tag="VYQ24_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1968967.1"
                     /translation="FITHIKLFAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQV
                     AMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
     gene            249..2087
                     /locus_tag="VYQ24_001870"
     CDS             249..2087
                     /locus_tag="VYQ24_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1968968.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTCLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKTLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEVGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            2100..>2275
                     /locus_tag="VYQ24_001871"
     CDS             2100..>2275
                     /locus_tag="VYQ24_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1968969.1"
                     /translation="MTIFPDDFLWGGAVAASQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGI"
BASE COUNT          727 a          309 c          473 g          766 t
ORIGIN      
        1 ttcataactc atataaaatt atttgcccat cgcttggttg aaaatacaac ttattgtgac
       61 gatgatgatg aagacttgtt agcattaatg aaaaataaat atcctagaga atatgaatgt
      121 ggtgaacagg tggcaatgtt tatacaaacg gagtacaatt atttgctgac ttctagtgaa
      181 ttagtttatt tgatggctca tattcgtcgc ttaacaaaaa atttagatta atagaatagg
      241 agaataccat gagttataaa gatacggtac aaaaaatcct cgatgtaatt ggaggtgaaa
      301 aaaatgtcaa tagagttacc cattgtgtaa catgtttaag attagaatta aaagatgaaa
      361 atttagtcaa tgatgatgat gtgaagaaga taccaggtgt aataggtatt atgaaaaaga
      421 atggacaata tcaaattata cttggtaatg atgtagctaa ttattataaa gaattcgtta
      481 aacttggcaa ttttgaatcc gattcagttg ttcaagggca caaagggaat attttagaaa
      541 gaatcattga gtatatcgct ggttccatga ctccaatcat tccagcaatg ttagggggag
      601 gtatgctgaa agtcttggta attattttac caatgcttgg tatattgcaa tcagattctc
      661 agactattgc ttttttgaca ttttttgggg atgctccata ttatttctta ccgctgttat
      721 tagcttattc tgcatcacaa aaattaaaag taacatctac attagctatg tctgtagcag
      781 gtgtacttct ccatccaaat tttgttcaaa tggtgcaatc agggaatcct cttagtttat
      841 ttggtgtacc tgtgacacca gctagttatg gttcgtcagt cgttccaatt cttattatgg
      901 tttggttgat gaaatatatt gaaaaaatga ttgctaaatt aacaccagct gttactaaga
      961 gttttttgca acctacgcta gtattattag tatcaagctg tattgcctta gttgtagtcg
     1021 ggcctattgg agtaattgtt ggtgaaggat tatcaaatct agttgggcaa atgtatggtg
     1081 tagctggatg gcttacatta gctattcttg gtgctattat gccatttatt gttatgactg
     1141 gaatgcattg ggcttttgca cctatttttt tggcggcatc tattgctact ccagacgtat
     1201 taattcttcc agcaatgtta gggtcaaact tagctcaagg ggctgcttcg atggctgttg
     1261 cattaaagag taaaaataat aatacaaaac aaattgcttt tgcagcaggt ttctcagcct
     1321 tacttgcagg gattaccgaa cctgcattat atggtgtgac tttaaaatat aaaaaaacgc
     1381 tttatgcagc tatgattggt ggtggattag cgggattatt tgcaggtctt actagtgtta
     1441 aagcgtatct atttgctgtc ccatctttga tagcgttgcc tcaatttatt tattctgatg
     1501 tgccatcaaa tattgtaaat gctttaattg tggcggtcat ttcggttgtt attacctttg
     1561 tattagctta tatatttgga atcgatgaag aagagagttc tagcaattta gaagttgaag
     1621 ttggagtttc aaataaaaaa atgatatttt ctcctatatc aggagaaatc attccgttaa
     1681 gcgatgtcca ggataaaaca ttttcagata aactaattgg agacggagta gcgattatcc
     1741 caagtgaagg taaggtttat gcaccatttg atgggaaaat tacaaatatt tttccgacta
     1801 agcacgcaat tggattgaag agtgatgagg gtgttgagtt actaattcat attggattag
     1861 atactgttga gctaaaaggt caaggtttta ttagtcatgt agaagaagga gacagagttt
     1921 tcaaaaatca gttgattttt gaaatggact tgaatttaat caagactaaa ggctacgaaa
     1981 cagttacacc agtaattgta acgaatacca atgattttct agatgtatta gtattaccta
     2041 ataatcagac aatcgagcat tctaaggaat tactggtaat attataatgg aggaaagtta
     2101 tgaccatttt tccagatgat tttctttggg gtggagctgt tgcagctagt caagtagagg
     2161 gagcatataa tgaagatggt aagggattat cagttcagga tgtgttaccc aaaggtggat
     2221 taggagaagc aacagaaaat cctacagaag ataacttaaa attgttaggt attga
//
LOCUS       DAVMEI010000190         2254 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVMEI010000190 DAVMEI010000000
VERSION     DAVMEI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.192"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            2..826
                     /locus_tag="VYQ24_001872"
     CDS             2..826
                     /locus_tag="VYQ24_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1968970.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            839..2005
                     /locus_tag="VYQ24_001873"
     CDS             839..2005
                     /locus_tag="VYQ24_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1968971.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
BASE COUNT          667 a          442 c          486 g          659 t
ORIGIN      
        1 aatgaatcaa tcctactttt atctaaaaat gaaagaacac aaactcaagg ttccttatac
       61 aggtaaggag cgccgtgtac gtattcttct tcctaaagat tatgagaaag atacagaccg
      121 ttcctatcct gttgtatact ttcatgacgg gcaaaatgtt tttaatagca aagagtcttt
      181 cattggacat tcatggaaga ttatcccagc tatcaaacga aatccggata tcagtcgcat
      241 gattgtcgtt gctattgaca atgatggtat ggggcggatg aatgagtatg cggcttggaa
      301 gttccaagaa tctcctatcc cagagcagca gtttggtggt aagggtgtgg agtatgctga
      361 gtttgtcatg gaggtggtca agccttttat caagcacaaa acaggttggt ttgatggcat
      421 gatgacgaca ggttgctcta tgggagccta tcatgcactc aatttcttcc tccagcatcc
      481 agatgtcttt accaaagtga ttgctctcag tggtgtttac gacgcacgtt tctttgtcgg
      541 cgattactac aacgatgatg ctatttacca aaactcgcca gtagattata tttggaacca
      601 aaacgatggc tggtttattg accgttaccg tcaggcagag attgtgctgt gtacggggct
      661 tggagcctgg gaacaagatg gtttaccatc cttttacaag ctcaaagaag cctttgacca
      721 gaaacaaatt ccagcctggt ttgctgaatg gggacatgat gtcgcccacg actgggaatg
      781 gtggcgtaaa caaatgcctt atttcctcgg taatctctat ttataaaagg agttacctat
      841 gaattacctt gttatttctc cctactatcc acaaaacttt caacagttta ccatcgaact
      901 agctaataaa ggcatcacag tcttgggaat tggtcaagag tcttacgagc aattggatga
      961 gcccttgcgc aatagcttga ccgagtattt tcgtgttgat aatcttgaga acatagatga
     1021 agtcaaacgt gcagttgctt ttctctttta taaacatggt ccaattgacc gcatcgagtc
     1081 tcacaatgaa tactggcttg agctagacgc aacactcaga gaacaattca atgtttttgg
     1141 tgccaaacca gaggatctca aaaagacgaa atataagtct gaaatgaaga aacttttcaa
     1201 aaaagcaggt gttcctgtgg tacctggagc tgttatcaag acggaagcag atgttgatca
     1261 agcagtgaaa gaaatcggtc ttccaatgat tgccaaacct gataatggag tgggagcagc
     1321 cgcaaccttt aaacttgaga cagaagacga tatcaatcac ttcaagcaag aatgggacca
     1381 ttcaaccctt tatttctttg aaaaatttgt cacttccagc gaaatctgta cctttgacgg
     1441 gctcgtggac aaggatggaa agattgtctt ctcaacaacc tttgactacg cctatacacc
     1501 gcttgacctc atgatttata agatggacaa ttcttattat gtgctcaagg atatggatcc
     1561 taaactgcgc aagtatgggg aagcaattgt caaagaattt ggtatgaaag aacggttttt
     1621 ccatattgag ttcttccgtg agggggacga ttatattacc atcgagtaca ataaccgccc
     1681 tgcaggtggt tttaccattg atgtttataa ctttgctcat tccttggacc tttatcgtgg
     1741 ctatgcagct attgtcgcag gagaggagtt cccggcgtca gactttgaaa ctcagtattg
     1801 tttggctact tctcgccgtg caaatgctca ctatgtttat tcagaagagg atttgcttgc
     1861 caaatatagc cagcagttca aggttaaaaa agtcatgcca gctgccttcg cggaacttca
     1921 aggagattac ctgtatatgc tgacaactcc gagtcgacaa gaaatggatc agatgattgc
     1981 tgatttcgga caacgtcaag aataagaact atcggattaa ggaaattcac tcccttaatc
     2041 cttttgtttt gtttggtaaa aaataagagc atcctaacaa ggtagctatc ataaaacttg
     2101 ttcgataact atttgaagca ggattaggtg gctagaaatt gaattttaac atttcaattt
     2161 agtcatagta ttgtgttttc gtatccttaa atcagctaaa aggatccatg acgacaccta
     2221 tacgatatag ttttcaggat accaaaccag tcta
//
LOCUS       DAVMEI010000191         2250 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVMEI010000191 DAVMEI010000000
VERSION     DAVMEI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.193"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            14..1768
                     /locus_tag="VYQ24_001874"
     CDS             14..1768
                     /locus_tag="VYQ24_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968972.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1734..>2250
                     /locus_tag="VYQ24_001875"
     CDS             1734..>2250
                     /locus_tag="VYQ24_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1968973.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPS"
BASE COUNT          783 a          311 c          424 g          732 t
ORIGIN      
        1 aggagatatg cttatgaaga tttacaaaaa actatttgct tatgtccaag ataagaaata
       61 tcttggggtt ttggccataa ttttttctgc tatatctgct gcacttacag tatatggata
      121 ttatttaatc tacaaatttc tagataagtt aataattaat tcaaacttat ccggtgcaga
      181 gagtatagca ttaaaatctg ttattacact aacaagtgga gcgatatttt attttgtctc
      241 aggaatgttt tcacatatct tgggatttag gcttgaaaca aatttaagaa aaaggggaat
      301 cgatggtctg gaaaaagcaa gttttaggtt ctttgactta aatccatctg gtcaaataag
      361 aaagatcata gatgataatg cagcacaaac tcatcaggtg gtagcacaca tgattcccga
      421 tagttctcag gcaataatca cacccgtact tgtacttgca cttggcttta tagtaagtat
      481 aagggttggc ataattttgc ttgctcttac tataattggt ggcttaattt taggggcaat
      541 gatgggcgag caagaattta tgaagatata ccaagaatcc ctatctaaac taagtgctga
      601 aactgttgag tacgtgagag gaatgcaagt tgtaaaaata tttaaagcaa atgtagagtc
      661 ttttaaaagc ttttataagg cgataaaaga ttactcaaag tatgcttatg attattccct
      721 atcttgtaaa aggccttatg ttttgtatca atggttattt tttggactga ttgcaatttt
      781 aattattcct atagtttatt ttatgactag cttagctagc gcaaaggtga ttttacttga
      841 gcttatcatg attttatttt tatcaggagt tctctttgtt tcattcatga gaatgatgtg
      901 gtactccatg tatatttctc aaggaaatta tgcagtagat actttagagg cgctttacga
      961 agatatgcaa aaagacaaat tagtgcatgg taatgtcaat aattttaaaa actataatat
     1021 agaatttgag aatgttagct ttgcttataa tgataaagct gtcattgaaa atttatcctt
     1081 taatttagaa gaaggaaagt cctacgcact tgtcggttca tctggatcag gcaaatcaac
     1141 agtagcaaaa cttatatcag gtttttacaa tgttaataaa ggaagcataa agataggcgg
     1201 gatagcaata agtgaatatt ctgacgaagc cttaattaaa gccatttcct ttgtttttca
     1261 agattcaaaa ttattcaaga agagcattta tgataatgta gctttagcta ataaagatgc
     1321 gacgaaagat gacgttatga gagccttaaa attagcagga tgcgatttaa tattagacaa
     1381 attcccagaa agagaaaata caatcatagg ctcaaaaggt gtttatttat ccggtggaga
     1441 aaaacaaaga attgcaattg ctagagcaat tttaaaggat tccaaaatta ttattatgga
     1501 tgaagcatca gcatctattg acccagataa cgagtttgaa ttgcaaaaag cttttaaaaa
     1561 tcttatgaag gataaaacag ttatcatgat tgcacacagg ctatctacaa ttaaagacct
     1621 tgatgaaatt attgtcatgg atagtggaaa aattatagaa agagggtctg acaaagaatt
     1681 aatgtcaaaa gatacaaggt ataagagcct gcaagagatg tttaacagtg cgaatgaatg
     1741 gagggtttca aatgaaagag ttttataaaa aaagatttgc tcttacagat ggaggagcaa
     1801 gaaatttaag taaagcaaca ctggcttcat ttttcgttta ttgtataaac atgcttcctg
     1861 ccatattact tatgattttt gctcaggaag ttttggaaaa tatgggcaaa agcaatggct
     1921 tttatatagt attctcagtt ttgactttga tagcaatgta tattttgctt tctatcgaat
     1981 acgataaatt atataacaca acctatcaag aaagtgcaga tttaagaata aggacagcgg
     2041 agaatttatc aaaattacct ctatcttact tttctaaaca tgacatttcc gacatttcac
     2101 aaacaatcat ggctgatatt gaaggcatag agcatgcaat gagccactca ataccaaagg
     2161 tgggcggcat ggtactgttt ttcccattaa tatctgtaat gatgctagcg ggcaatgtca
     2221 agatgggttt agctgtaatt attccatcta
//
LOCUS       DAVMEI010000192         2201 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVMEI010000192 DAVMEI010000000
VERSION     DAVMEI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.194"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            144..1151
                     /locus_tag="VYQ24_001876"
     CDS             144..1151
                     /locus_tag="VYQ24_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1968974.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPDFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQNRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1154..1354
                     /locus_tag="VYQ24_001877"
     CDS             1154..1354
                     /locus_tag="VYQ24_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1968975.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1506..1688
                     /gene="rpmF"
                     /locus_tag="VYQ24_001878"
     CDS             1506..1688
                     /gene="rpmF"
                     /locus_tag="VYQ24_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1968976.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1704..1853
                     /gene="rpmG"
                     /locus_tag="VYQ24_001879"
     CDS             1704..1853
                     /gene="rpmG"
                     /locus_tag="VYQ24_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1968977.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
BASE COUNT          732 a          420 c          411 g          638 t
ORIGIN      
        1 aagagtattt gagcacaata taatctcacc ttttaaaaga aattattggg ctgaaagttt
       61 ttagtgatta tttttcaact ttctattgac ttttaaaaac aaagtgatat catttagata
      121 tcaaataatg ggaggtcatt cttatgactg aaaaactaat caattcaaaa ccaaatggtg
      181 tattcgcatt gattctcatt gaattgacgc tcgtacttgg tatctttata tttataatgg
      241 gtgttggttc ggaaaacatt tttggaatta ttatcggacc tttactaatc gtaattgcag
      301 gtctagctca tgctggttta aaagttgtca aacctcagga agctctggtt ctgacactct
      361 ttggtaatta tacaggtacc atcaaagaac ctgattttta ctttgtcaat cccttcagcg
      421 tagcagtcaa ccctgcaaac cacactcgac ttggacaaag tggtgatgtt agcacaaaat
      481 ctcctttttt aggtgctaaa tcatcaaatg acaatgatgt aaatcttgaa attggcaaga
      541 aacacatttc cctcaaagtc atgaccttga gcaattctcg tcaaaaaatc aatgattgct
      601 taggaaaccc tgtagaaatc ggtatcgcgg taacttggag agttgtcgat accgctaagg
      661 cagtcttcaa cgttgataac tacaaagaat atctttcatt gcaatgtgat agcgccctcc
      721 gtaatattgt ccgcatctat ccttacgatg tgtctcctaa tgtggatact acgggtgacg
      781 ggcaagcaga tgaaggtagt ctccgtggct ctagcgaaat tgttgctaac cgtattcgtg
      841 aagaaatcca aaatcgtgtt gaggatgctg gcttggaaat ccttgaagca cgtatcactt
      901 acctagctta tgctccagaa attgctgccg ttatgcttca acgccaacaa gcatctgcca
      961 ttattgatgc acggaagatg attgtagatg gtgctgtagg aatggttgaa atggcactag
     1021 aacggctcaa tgaaggggaa ttggtagaac ttgacgaaga acgaaaagct gccatggttt
     1081 caaatctcct tgtcgttctt tgtggcaatc atgatgcaca accaattgtc aacacaggaa
     1141 gtctttacta aagatggctg aagctaaaaa aaagcagatc ccccttcgac tctcaacaaa
     1201 gttatacgct gcactcgcat catgggcaga agacgacttt cgttcagtca atgggcaaat
     1261 cgaatatctc ctcacagagt gtgtcaagca acggaagaaa gacggaaaat acgtatcaga
     1321 aaccattgac gaaccatttg agattgatat ttaacacctt ctcctgtctg ccgtgtcgga
     1381 taggagaatt ttttcatctt tttttgtcaa gtaactttac tttacaaaaa aaatgtgtta
     1441 tcctagtatg gttgatgaaa atcagtagat tgaatcgaat ataaatacct aaaggagaaa
     1501 tcaaaatggc agtacctgca cgtcgcactt caaaagcgaa gaaaaacaaa cgtcgtacac
     1561 actacaaagt aacagctcca tctgtaaact ttgacgaaac tactggagat tactcacgtt
     1621 ctcaccgtgt atcacttaaa ggatactaca aaggacgtaa aatcgctaaa gctgcatcag
     1681 ctgaataata gaagggagat accatgcgcg taaatattac acttgaacac aaagaatctg
     1741 gtgaacgctt gtaccttact tctaaaaaca aacgtaacac tccagaccgc cttcaattga
     1801 agaaatactc accaaaactt cgcaaacacg ttgtgtttac agaagtgaag taagcattca
     1861 atacgaatca atacagaaga aaaacgcttg atcagcgttt tttctttttg ttaatttcac
     1921 tatattgcaa gagaaatcat tcgaatgact acattttttg aaataagaat acggattcgg
     1981 acaattaaaa gcaagaggaa ctcaaatgta agttggggtt ccttttttgt atatttttca
     2041 aataacgcct ctacactatt tgtagcaaat tcaccaacta cagttgtatc ttagttaaaa
     2101 taagttagaa tatgtaagtg agtaccagat ataccaagac atcgtcacca tctaaggtat
     2161 attcaaaata caaaagttga ccaactagat ttctgaatat c
//
LOCUS       DAVMEI010000193         2176 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVMEI010000193 DAVMEI010000000
VERSION     DAVMEI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.197"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..517)
                     /gene="psaA"
                     /locus_tag="VYQ24_001880"
     CDS             complement(<1..517)
                     /gene="psaA"
                     /locus_tag="VYQ24_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1968978.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKN"
     gene            complement(549..1397)
                     /locus_tag="VYQ24_001881"
     CDS             complement(549..1397)
                     /locus_tag="VYQ24_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1968979.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(1394..2116)
                     /locus_tag="VYQ24_001882"
     CDS             complement(1394..2116)
                     /locus_tag="VYQ24_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1968980.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT          652 a          418 c          464 g          642 t
ORIGIN      
        1 gatttttttc atagaattct ttattgttag ggtctttggc gctcaattgt ttggcgatat
       61 ttttagcaaa aataataccg ttttcaaggt taagccaagc gtgtgggtct tcttttcctt
      121 tttcattttg accttcaagg tagataacat caacgccgtc gctgactgcg aagtagtctt
      181 tgttttcagt tttcttggca ttttctacca attttgtaaa ccaagcattg ccacctgttt
      241 caaggttgat accgttatag aaaatcaaat cagcctcaga agttttctta acgtcttcag
      301 gaagtggttc gtattcgtgt gggtcttgcc caatcggaac gatactatga aggtcaattt
      361 tgtcaccagc aatattttta gtaatatcag cgatgattga gtttgtagca acaactttta
      421 gtttttgacc agaagttgta tctttttttc cgctagcaca tgctacaaga atgattgcag
      481 aaagaaagag aacgagtaat gtacctaatt ttttcattag atcctccaat ttattagggc
      541 tttgcccctt attttaacaa atgtttattt ttcagtttca aatatcgttg tttgggagcg
      601 ataaagaagc taatgagaaa gaaactagca gctgtaagca cgatactaga acctgccgca
      661 acattaaaac tatagccaat aaagagtccc aaaactgaag cagtagctcc gaaggttgag
      721 gaaaggaaaa tcatgctttt cagactatta gcatacagat aagcagttgc agctggggta
      781 atcagcatgg ctacaatcag gatagttccg acactttgca tggctgtcac agacacgaga
      841 gtcaggagta ccatgagaag gtagtgatag aaattgacag gcattcccat ggctttagcc
      901 aagagttcat caaaggaagt tatcaagagt tgcttgaaga aaatccagat taacaagagg
      961 atggctgccc ccacacccat agtaataaac atatccgtat cttggacggc cagaatatta
     1021 ccaaaaagga tatggaaaag gtcagttgaa cttttagcga caccaatcaa gatgataccg
     1081 agggctaaga aagaagaaaa ggtaatgccg atggcggtat cgcttttgat aatcgagttt
     1141 cctttgatgt aggtaatgat gatggcagct agcaatccaa agacaatggc tccgataaag
     1201 aagtcaaggc ccaagatgaa ggagagggct acacctggta agacagcatg tgaaatggca
     1261 tctcccatga gtgacatccc gcgtagaata atgaaacatc ccacggctcc agctacaatc
     1321 ccgacgacaa tagctgttat caaggcattt tgtaggaaat ggaatttttg caatccatcg
     1381 ataaattctg caatcatagg tcacctccat tgaaaaagag ttgattaccg taagcttctt
     1441 ttagattggt ttcggtaaaa gtttcttttg ttggaccaaa ggcaatcact tctcgattga
     1501 caagtaagac ttgatcgaag tagtggggaa tcttgctgag gtcgtggtga acgatgagaa
     1561 ccgtcttccc agcttttttc aaatctctca gcgtattcat gatgatttcc tcactgacag
     1621 agtcaatccc agcaaagggt tcatccaaga ggatatagtc ggcttcctgc accaaacatc
     1681 tggcaatcaa gacccgctgg aattgacctc cagacagttg actaatttga cgttcagcgt
     1741 agtcagctag gccgacgatt tcaagggcct cttgcacttt cttccaatgt ttagccttta
     1801 aacttcgaaa gagaggaata gagggaaata gtcctaacga gacgcattcc ttgaccttga
     1861 tgggaaagtt gtagtcgata ttgatttttt gttcgacata ggcaattcgg tgtaaggatt
     1921 ttttaacttc cttgtcatcg agaaatgcct gaccttgatg tgggataatt cccagcatac
     1981 cttttaatag tgttgatttc ccagcgccgt ttggaccaat gatgccggta attgttggtc
     2041 catggagcac tagtgaaata tccttaagtg ccaacgtttc tttgtaggag acactgaggt
     2101 tttcgatacg tatcataaac ttgtattcct cctgtctctt aatatacatt aaaaaaaaaa
     2161 ttaagtcaag ttaatt
//
LOCUS       DAVMEI010000194         2114 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVMEI010000194 DAVMEI010000000
VERSION     DAVMEI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.198"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1668
                     /locus_tag="VYQ24_001883"
     CDS             <1..1668
                     /locus_tag="VYQ24_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1968981.1"
                     /translation="KIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIVGEYE
                     DAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIMQDYG
                     VNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNGGFAP
                     YGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNGKNPL
                     FDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDE
                     VWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKY
                     KDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMK
                     VDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDK
                     IDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISALISEI
                     QVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            1936..>2114
                     /locus_tag="VYQ24_001884"
     CDS             1936..>2114
                     /locus_tag="VYQ24_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968982.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEII"
BASE COUNT          792 a          266 c          471 g          585 t
ORIGIN      
        1 aaaataaaag tatacctcta tacacgagta tctacgtcaa tacagataga ggggtattcg
       61 ttagaggcac aaaaatcacg aatgaaagct tttgctattt acaacgatta tgaaattgtt
      121 ggagagtatg aagatgccgg gaagtctggc aagtctattg aaggtagaat acagttcaat
      181 cgaatgatgg aagatataaa atctggaaaa gatggagtat cttttgttct tgtgtttaag
      241 ttatcaagat ttgcaagaaa tgccgctgat gttctttcta cacttcagat aatgcaggat
      301 tatggagtta atcttatctg cgttgaagat ggcattgatt catctaaaga tgctgggaaa
      361 ctaatgattt ctgttttatc agctgtggct gaaatagaaa gagaaaacat ccgtattcaa
      421 acaatggaag gttgcattca aaaagcaagg gaaggaaaat ggaatggtgg ctttgctccg
      481 tatggatata aacttgaaga cggcaagctg tttataaatg aggaagaggc agttgcaata
      541 agaacgattt tcgaccagta tgtaaatact acgataggag ccaatgggat atctaaatac
      601 ttagagaatc atggaattag aaaaatccca agacagaatg gtaagaatcc tttgtttgat
      661 gcaggtctta taagaaagat attaaagaat cctgtatata atgggaaaat agcatttgga
      721 agaagaactt tagaaaaagt tcatggtaca agaaatgaat ataagcaggt tgaacaagat
      781 gaatatctaa tatctgaagg gatacatgaa gctatagttt ccgatgaagt ttggcaagct
      841 gctcaggtta agctaaaatc tcaagcaaag aaatatgagc atgtgaataa aggaaaagat
      901 acacgcacac acttgctttc tggaattgta aaatgcccga tatgtggagt gggaatgttt
      961 gggaacaagt gtatcaagaa aaagaaagat ggcacaaagt ataaagattt ttattactat
     1021 ggttgtaaac ataggcagat gataagaggt cataagtgta cattcagtaa gcaaattaga
     1081 gaagaattgt tagatgatgc tgttgcagag gtgattgtca agatagtaag taatccgaaa
     1141 tttgcttcta tgatgcaaga aaaaatcaac atgaaggtgg atacctctga aatagaaaaa
     1201 gaaatagata attaccaaaa ggaattgaga aagagtcatt ctacaaagtt taagctaatt
     1261 gaggaaatag ataatttaga tgttgaagat aagcactata agagaaggaa acaggattta
     1321 gatgatagac tatatcgtat gtatgacaaa atagatgaat tagaatcatc actaattgat
     1381 gcgaaagcaa agaaacagac tattgaagct gaaaaactta caggagataa catatataag
     1441 gttctgatct attttgataa gctctataaa gtcatgaatg atgtagagcg taggcagtta
     1501 atttcagctt tgatttctga aattcaagtt tatgaagaaa aacaatctaa cggacaatgg
     1561 ctaaaatcca ttacttttaa gctaccaatt atagaagaaa atctcaacat agatttggac
     1621 aatgatgaac aagttgaatg tgtaagttta ttagagaaac gtagttaatc ctcaaaaggt
     1681 tccccaaact ttttgagtcc cactgacgca tcacgctgag gcggtatcac tgcttgtacg
     1741 agttgaagca tcagcgaagt aaaagtagat gttcagccaa tgcgacgagg tgagaccgaa
     1801 ggtcgataag gtgtgttctg ggtataggac tcgtaaagta agtaggaaat atgatgagat
     1861 gatacagaga gcctgaattg gtcaggtgac gtgcttattt acattagcct gataatgaaa
     1921 atggagagat tatcgatggt tatattggaa ttgtaccaaa atgattattc taaagattta
     1981 gtagcatttg attccataga agatgggaaa gcctttgtag ctcaaatccc gggatacact
     2041 ctggaaacag aagatggttt tgaagtggag tattttaatc ctaaaaacat accggattat
     2101 atggaaatta tttt
//
LOCUS       DAVMEI010000195         2107 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.199, whole genome shotgun sequence.
ACCESSION   DAVMEI010000195 DAVMEI010000000
VERSION     DAVMEI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.199"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            66..230
                     /locus_tag="VYQ24_001885"
     CDS             66..230
                     /locus_tag="VYQ24_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1968983.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            309..1037
                     /gene="yaaA"
                     /locus_tag="VYQ24_001886"
     CDS             309..1037
                     /gene="yaaA"
                     /locus_tag="VYQ24_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1968984.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1315..1863)
                     /locus_tag="VYQ24_001887"
     CDS             complement(1315..1863)
                     /locus_tag="VYQ24_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1968985.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
BASE COUNT          642 a          381 c          459 g          625 t
ORIGIN      
        1 aaaaaggttt atttaataaa tatatgttat aataaggtaa attaagccta atggaggaca
       61 aagaaatggg tttttatttg atggttgctt caatgctact cggactgctc gctttgaaga
      121 taggtttttc tcagttcaag gagaaaaaag ataaattctt atctatctta acaagtttag
      181 ctggcacagc ccttgttctc gtggctgtct ggttaggatg gcccaaataa atagaaaaac
      241 ctcggtcaaa ccgaggtttt tcaaatgaat ttcttggttg tggctaaaaa atatgctaca
      301 ctatcaatat gaaaatttta atcccaacag caaaagaaat gaacacagac ttgccaagta
      361 tcgaggcaat tcctttaaaa ccagaaagtc agaccgtgct tgatgccttg gctctctatt
      421 ctgccagtca attggagagt ttctacaagg tatcggctga gaaagcggcg gaagaatttc
      481 aaaatatcca agctttgaaa aggcaaactg ctcaacacta tccagccttg aaactttttg
      541 atgggcttat gtaccgcaac attaagagag ataagctgac cgaggcggaa caagattatc
      601 ttgaaaatca tgtcttcatt acctcggctt tgtacggtgt tgttccagcc ttgtcaccca
      661 tggctcctca ccgtttggat tttttgatga aattaaaagt cgctggtaag actttgaaga
      721 gccattggaa ggcagtctat gatgaagctc tgaagaagga agaagtgatt ttctctctct
      781 tgtcatcaga gtttgagact gtattttcta aggaaatcag agcaaagatg gtgaccttca
      841 aattcatgga ggatagaggc ggtcagctga agattcactc aactatctcc aagaaagcgc
      901 gcggggcctt tctaacagct ttaatagaaa atcaagtaca aactgtgggg gaagcacgtc
      961 gcttgaactt tgctggattt gtttaccgag aagatttgtc acaaccacag ggattggttt
     1021 ttgtaaagga agtatagaaa tcagatattt tgtatttggc aatatgtatc atcagctgtt
     1081 ttagtttata aatgagaaaa aaagcgaaca agctagtctt ctgagtgtca gaaaagtagt
     1141 cttgttcgct tttattatgt tatactcttc gaaaatctct tcaagccacg tcagcgtcgc
     1201 cttaccgtat atatgtcact gacttcgtca gttctatcta caacctcaaa gcagtgcttt
     1261 gagcagcttg cggctagctt cctagtttgc tttttgattt ttattgagtg ttagttactt
     1321 gatagcttcg accaagtctt gagcttgttt ttcaagtgag tttaggactg tttcttcaag
     1381 aaccaatttt ccgtctgccc aggcagagtc attaacacgt gcagcagtga aatcaccaac
     1441 gccttgtgta cggataaatg gcaagaggtc tttgtagata gcgaaaagtt gatcgtgccc
     1501 tgcattggct acagatgata ctgtgacaaa cttgtcttga agggcagaaa cgccacgtgt
     1561 atcagacaag tcaagggcac gagatagcca gtcaagcaag tttttcactg taccagggat
     1621 agagaagttg tagactggag agaaaatcca gatagcatcc gcaacgagaa ctgcttcacg
     1681 agcagcagct acagctggat gtgttggaac ttccaaatct tggctgaaga gaggaagggc
     1741 tgaataatca aggtagctaa cttccgcttt accagcaagt gctttctcag cttcgagcgc
     1801 catttggtgg ttgaaagaac cttgacgtag tgatccgacg ataaataata cttttttaga
     1861 catgatgtgt ctcctttttc ttaaaatttc gagaactctc tcgttgttat aaaatatatt
     1921 acaacaaaac aaaagaactt gcaagaaatt tgctcagagt ttatttgaaa attgtaaaat
     1981 ttcggaaact ctagtcattt gtgactgaaa aagtaagaac tgtttcactt tcaaaatcgg
     2041 ttttgtttga agggagaaat agaattggtt gctcaaagca cagctttgag gttgcagata
     2101 aagctga
//
LOCUS       DAVMEI010000196         2092 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVMEI010000196 DAVMEI010000000
VERSION     DAVMEI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.200"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            15..1076
                     /gene="dinB"
                     /locus_tag="VYQ24_001888"
     CDS             15..1076
                     /gene="dinB"
                     /locus_tag="VYQ24_001888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1968986.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            complement(1223..2068)
                     /locus_tag="VYQ24_001889"
     CDS             complement(1223..2068)
                     /locus_tag="VYQ24_001889"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1968987.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          703 a          426 c          414 g          549 t
ORIGIN      
        1 aaaaggtgct aggcatgttg atttttcctt tgttaaatga tttgtcaaga aaaatcatcc
       61 atattgacat ggatgccttt tttgctgcag tggaaatcag ggataatcct aaactcagag
      121 gaaaacctgt cattattgga agcgaccctc ggcaaacagg tggacgggga gtcgtttcta
      181 cctgtagtta tgaggcaaga gcttttggtg tccattctgc catgagttcc aaggaagctt
      241 atgaacgttg tccccaggct gtctttatct cagggaatta tgagaaatac aagtctgtgg
      301 gactccagat tcgagctatt tttaagcgct atacagattt gattgaaccc atgagcattg
      361 acgaagccta tttggatgtg acagaaaata aactcggtat caagtcagcg gtcaaaattg
      421 ctcgcctcat tcaaaaagat atctggcaag aactccatct aactgcttcc gcaggcgttt
      481 cttacaacaa attcttagct aaaatggcga gtgattatca aaaaccacat ggtttgacag
      541 tgattctacc tgaacaggct gaggattttc tcaaacaaat ggatatttcc aaatttcatg
      601 gagtaggaaa aaagacagta gaacgtcttc atcaaatggg cgtttttact ggtgctgatt
      661 tacttgaagt tcctgaggta accctaatag accgttttgg tagactaggc tatgatctgt
      721 atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc caatcgcatc cgtaaatcaa
      781 tcggcaagga gaaaacctac gggaagattc tccttgctga ggaagatatc aaaaaagagc
      841 tgactcttct atcagaaaaa gtcgctctca atctacatca acaagaaaaa gctggaaaaa
      901 ttgtcatttt gaaaatccgc tacgaggact tttcaactct taccaaacga aaaagtcttg
      961 ctcaaaaaac acaagatgct agtcagataa gccaaatagc cctgcaactc tatgaagaat
     1021 taagcaagaa agaaagaggt gtccgcctat tggggattac catgactgga ttttaaagct
     1081 caatgaaaat caaagagcaa actaggaggc tagccgcagg ctgctcaaaa cactgttttg
     1141 aggttgcaga taaagctgac gcggtttgaa gagattttcg aagagtataa aaccttgaag
     1201 agttgcccct tcaaggtttt tcttatacaa ataaacggac aaaactgtaa agtagcaaaa
     1261 cactaccaag cacgatacgg tatttaccaa aaagggtgaa gtcgtgtttt ttcacatagc
     1321 tggtcaagaa gcgaatagcc accatgctga ccgcaaaagc tactcccatc gcgaccaaga
     1381 gtaaaaacaa ttgcccaaag ctcaagagtt ctccggcttt cacaaatttg aaaatcttta
     1441 aggcactagc tccaaacata acgggaatcc caagatagaa ggtaaattct gtcacaactg
     1501 aacgactggt tccatttaac aaaccaccga caatcgttgc acctgaacgg ctagtccctg
     1561 gtaaaagagc aagaacttgg aagagtccga tatagaaagc ggtcgtataa ggaagcttgt
     1621 ccaactctgt tacacttggc tcgatagcac gcgctttatt gcgcttttcc aaatatatga
     1681 aggcaacccc gtagataatc aacatgagag caactgaaac catgttatgg aagtgggtat
     1741 caaaccaatc atcaaattta aagacaccaa gtaaaggtaa agtagcaatc aagaccttca
     1801 accatagtct ccaagtctta cgaacttcct gtttgtcctt agtcggttta aaaggattga
     1861 gcttgttaaa ataaatcacc ataactgcta aaatagcacc aagctgaatc acgacattaa
     1921 acatggacat aaaggcttca ttttgatttt ggtattggat aaactcctct gctaaaatca
     1981 agtgacctgt actggaaatc ggcaaccatt ccgtaattcc ttcaacaatc ccgaagaaga
     2041 tagattttaa aatttcaata agatacatat atttctcctt atattatatt ta
//
LOCUS       DAVMEI010000197         2053 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVMEI010000197 DAVMEI010000000
VERSION     DAVMEI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.201"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            146..1327
                     /locus_tag="VYQ24_001890"
     CDS             146..1327
                     /locus_tag="VYQ24_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1968988.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            1385..>2053
                     /locus_tag="VYQ24_001891"
     CDS             1385..>2053
                     /locus_tag="VYQ24_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1968989.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLETLLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVE"
BASE COUNT          752 a          357 c          374 g          570 t
ORIGIN      
        1 tattcacaaa ataaaaaata gatttcaatt aagaaaatca cagtttcaaa agagttatcc
       61 acagtttgtg taaaactttt gtgtttaagt tataattaag ctagtcagtt tatactttca
      121 gtaattcaaa catatggagg caaatatgaa acatctaaaa acattttaca aaaaatggtt
      181 tcaattatta gtcgttatcg tcattagctt ttttagtgga gccttgggta gtttttcaat
      241 aactcaacta actcaaaaaa gtagtgtaaa caactctaac aataatagta ctattacaca
      301 aactgcctat aagaacgaaa attcaacaac acaggctgtt aacaaagtaa aagatgctgt
      361 tgtttctgtt attacttatt cggcaaacag acaaaatagc gtatttggca atgatgatac
      421 tgacacagat tctcagcgaa tctctagtga aggatctgga gttatttata aaaagaatga
      481 taaagaagct tacatcgtca ccaacaatca cgttataaat ggcgccagca aagtagatat
      541 tcgattgtca gatgggacta aagtacctgg agaaattgtc ggagctgaca ctttctctga
      601 tattgctgtc gtaaaaatct cttcagaaaa agtgacaaca gtagctgagt ttggtgattc
      661 tagtaagtta actgtaggag aaactgctat tgccatcggt agcccgttag gttctgaata
      721 tgcaaatact gtcactcaag gtatcgtatc cagtctcaat agaaatgtat ccttaaaatc
      781 ggaagatgga caagctattt ctacaaaagc catccaaact gatactgcta ttaacccagg
      841 taactctggc ggcccactga tcaatattca agggcaggtt atcggaatta cctcaagtaa
      901 aattgctaca aatggaggaa catctgtaga aggtcttggt ttcgcaattc ctgcaaatga
      961 tgctatcaat attattgaac agttagaaaa aaacggaaaa gtgacgcgtc cagctttggg
     1021 aatccagatg gttaatttat ctaatgtgag tacaagcgac atcagaagac tcaatattcc
     1081 aagtaatgtt acatctggtg tagttgttcg ttcggtacaa agtaatatgc ctgccaatgg
     1141 tcaccttgaa aaatacgatg taattacaaa agtagatgac aaagagattg cttcatcaac
     1201 agacttacaa agtgctcttt acaaccattc tatcggagac accattaaga taacctacta
     1261 tcgtaacggg aaagaagaaa ctacctctat caaacttaac aagagttcag gtgatttaga
     1321 atcttaattg acatctatgt aaagaaagct ttacataaga gaaaagatgt gttagtgtag
     1381 aatcatggaa aaatttgaaa tgatttctat cacagatata caaaaaaatc cctatcaacc
     1441 ccgaaaagaa tttgatagag aaaagctaga tgaactagca cagtctatca aagaaaatgg
     1501 ggtcattcaa ccgattattg ttcgtcaatc tcctgttatt ggttatgaaa tccttgcagg
     1561 agagagacgc tatcgggctt cacttttagc tggtctacgg tctatcccag ctgttgttaa
     1621 acagatttca gaccaagaga tgatggtcca gtccattatt gaaaatttac agagagaaaa
     1681 tttaaaccca atagaagaag cacgcgccta tgaatctctc gtagagaaag gattcaccca
     1741 tgctgaaatt gcagataaga tgggcaagtc tcgtccatat atcagcaact ccattcgttt
     1801 actttccttg ccagaacaga ttctctcaga agtagaaaat ggcaaactat cacaagccca
     1861 tgcgcgttcc ctagttgggt taaataagga acaacaagac tatttctttc aacggattat
     1921 agaagaagat atttctgtaa ggaagttaga aactcttctg acagagaaaa aacaaaagaa
     1981 acagcaaaaa actaatcatt tcatacaaaa tgaagaaaaa cagttaagaa aactactcgg
     2041 attagatgta gaa
//
LOCUS       DAVMEI010000198         2039 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVMEI010000198 DAVMEI010000000
VERSION     DAVMEI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.202"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            438..1913
                     /locus_tag="VYQ24_001892"
     CDS             438..1913
                     /locus_tag="VYQ24_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1968990.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSARKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          608 a          384 c          457 g          590 t
ORIGIN      
        1 tcaaaaaata ctttacaagt tcttaaaaac atgatatagt aataaagctt agaaaatgag
       61 atgatgtttt ctagcaaata taaacccgag taaaaaatgc ctacggacag gcagggttga
      121 atgccgaagc gtggttgaaa agccacatta ttgatagggt taaaagccta cttttataag
      181 ttgatgttag gacacttgtc ctaattcata aatttttagt gtggtgaaag cacacgtcat
      241 cttgtgaaac gatcaataaa gtacgtaata tttgctacta gagagttagg aaacatcggg
      301 aacagacata ctcaacagaa accaaaataa acacgtcaga agattgcaga gcaggtgaaa
      361 acctgctctt ttttcatgag tcaaccttta gttccttagt tttcataagg tcctaaaaat
      421 attgaaagga gtatgttttg aaagagttag atcaaaacca agccccaatt tatgaggcct
      481 tggtgaagtt acgcaagaaa aggattgttc cctttgatgt tccaggtcac aagcgtggac
      541 ggggaaatcc agaacttgtc gaactcttag gagaaaaatg tgtaggcatt gatgtcaatt
      601 cgatgaaacc cttggataat ttaggccatc ctatttcgat tattcgtgat gcagaggagc
      661 tggctgcaga tgcttttgga gctagccatg cctttctaat gattggtgga acaacttcat
      721 cggtgcagac tatgattctg gcaacctgca aggcaggaga taagattatt ctgccacgaa
      781 atgtccataa atctgctatc aatgcgttgg ttctatgtgg tgccattccc atctatatcg
      841 agatgagtgt agatcctaag attggtatcg ctttaggtct tgaaaatgac cgagtagcac
      901 aggccataaa ggaccatcca gatgctaagg ctatcctaat caacaatcct acttactacg
      961 gcatctgttc agacctaaag gggttgacag aaatggctca tgaagctggc atgatggttt
     1021 tagtagatga agcccatgga gcgcatttgc atttcactgg taaacttcca atttctgcta
     1081 tggatgcagg agctgatatg gcagcagttt ccatgcataa gtctggtggg agtttgacac
     1141 aaagttcgct tcttttaatc ggggagcaga tgaatcccga gtacgttcgt cagattataa
     1201 acctgaccca gtctacatct gcctcttact tgttgatggc tagtttggat atttcacgtc
     1261 gcaacttggc ccttcgtggt aaagagtcgt ttgagaaagt cattgagcta tctgagtatg
     1321 cccgccgtga aatcaatgct atcggtggct actatgccta ctcaaaagaa ttaatagacg
     1381 gtgtttcggt ttgcgatttt gacgtaacta agctgtcagt ttacactcag ggtattggct
     1441 taacaggtat cgaggtttat gacctcttgc gagacgaata cgacattcag atcgagtttg
     1501 gtgatatcgg caatatcttg gcctatattt ctatcggcga ccgcatccaa gacatcgagc
     1561 gcttggttgg tgctctggct gatattaaga gactctattc aagagatgga aaagatttga
     1621 tagcaggaga atatattcag cccgagttag tgctgtctcc gcaagaagcc ttctattcag
     1681 caagaaaaag tttaactttg gatgattctg ttggacaggt ctgtggagaa tttgtcatgt
     1741 gctatcctcc agggattcct atcttggctc ctggtgaacg cattacacga gaaattgtcg
     1801 actatatcca attcgccaag gaacgtggtt gctccctcca agggacggaa gatccagagg
     1861 tcaatcatat caacgttatt aagagaaaga caaactataa gaaaagtcaa tagttttatc
     1921 taaactattt cttatttcaa tttgatgatt tggcgatgat tttagagcac ggcaaaaagc
     1981 ccttgaatta gaagcggtca atcgcttaat ttctatcagc ttatcaaatc ctgcctcaa
//
LOCUS       DAVMEI010000199         2028 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVMEI010000199 DAVMEI010000000
VERSION     DAVMEI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.203"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(388..1281)
                     /locus_tag="VYQ24_001893"
     CDS             complement(388..1281)
                     /locus_tag="VYQ24_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1968991.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
BASE COUNT          698 a          404 c          215 g          711 t
ORIGIN      
        1 tcaaattact tttatatcac taagactcaa taaaagataa tgtctattta acttttatac
       61 gattactacc tcctttttag tttacacttc ctacatcata aataaacata ctcaaccctc
      121 acaatctcca aataactctt ctatatcatt gaaaattgag tttcatcctc tatactaatg
      181 taggaaacct attaaaacat tcctttaatc caatataaga ataccttgtt ccattaatct
      241 ctacccgata ttatagatag aaccaaattc ctatacttaa aaggacaaat aacgttccac
      301 ccataataaa cctcctttct ttttgagagc gttttcttta taggtttagt atagcaaaaa
      361 catgaaaaaa acaacatgac ttagaagtca ggttgttggg aaatctatct gtttttctgc
      421 taaaattctc tgaactaata cctgatcgtc tagcaattct gctagagaaa tcgccttttc
      481 atagaaactt tctgcctcta aaatattctt gtctacaaat aaagcatatt ttgcctttag
      541 caaaaataga tgaggtcggt aaaaaacatg tcttgtttta gcaataattt tctcaaatgt
      601 ttgaatatat atcagactat cttctgtctg aatttggata aataggctca gaagaacttt
      661 ttccatttga actaggctag ccttatcttc atattctgaa catagcaata ctttttgagc
      721 taactcctca aaatgttctt tatcttctaa tcccatagca caacaaaaga aatacaattc
      781 aataatcaac aaatcattta cagaaaaata tttctttttt aataactgct ggaaatattc
      841 ttccactaac cctaaaccgt aggtcacatc tgaactacca taaatatcaa aacgagcttg
      901 tgtcacatcc acaattaact gctcttcctc aggcaaattc tcataatagt tttcatatat
      961 ttcctcaagt atagcttctt ttcgttcgat tcttccctta tcagcgtagg tttgagaatg
     1021 aatcagctca tctttgagtt ttaggaatcc ttttggaatc tctataacct taacatccac
     1081 aatattctgt aatgatactc caagtttatc cgcaatcaat aataatttag gaagtgttgc
     1141 taaagcttgc ccattttcaa tacgtgctag ctgacgaatc gtcaactctg attcatcacc
     1201 acaaatatct atctgagttt ttccacaaga tcgcctaata gttttgattt tataaccaat
     1261 ttcacgtttc aactcatcca tagtaatact ccttaagttg attacaaaga tttctttttc
     1321 ttcctaattc ttttgtcaat catatataaa acgataagcg aaaacagcgg tagaacaata
     1381 taactctttt cactcattat cagctgaaat cccagggttg ctagtatctt aaagatgaaa
     1441 atattcatca aaaatagaaa aacaaatact aaaaaatatt ttaaaacctt cttcatttct
     1501 tttcttcaac tttcctaatt tctacaccta ttttatcgaa aatatatata aaactccaaa
     1561 taaacacttc gactgcactt tttttggcaa aaaatagaca gaacaaacat cctacctact
     1621 aaaaatatga tatgaaagga aaatgatttt ttactgacac cctaggcaag cttgggacaa
     1681 ataaatcatt gatagcaaca tttacgacat cctatttaaa gttccttcaa tttctaatgt
     1741 ccccgctatt tctaagccac ctctggtatg atctattcct agtcagaata gataacaccg
     1801 ttctaaatta cgattcatag ttactccttt tttgactcgt tactataaga ctcctattta
     1861 catgcgcaaa ataattttga ttcagattca tcatttatag ttttgattat tttttaacag
     1921 atttttctgt tttcttctcc gaagacttta aaaatttttt tctaattaaa tcatccatat
     1981 aaggaccaaa tataccaact actaaaccaa taataaaact tttaaaat
//
LOCUS       DAVMEI010000200         1991 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVMEI010000200 DAVMEI010000000
VERSION     DAVMEI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.204"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            159..1361
                     /gene="rpsA"
                     /locus_tag="VYQ24_001894"
     CDS             159..1361
                     /gene="rpsA"
                     /locus_tag="VYQ24_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1968992.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(1446..>1991)
                     /locus_tag="VYQ24_001895"
     CDS             complement(1446..>1991)
                     /locus_tag="VYQ24_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1968993.1"
                     /translation="QHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSD
                     KRFYRPTFRMHLTNKEILDKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNL
                     KQAHPIFQTVFKTFLKDKEKIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFK
                     KRIFIALNIKKERTKFVLSQA"
BASE COUNT          612 a          315 c          454 g          610 t
ORIGIN      
        1 ttaggtaata atcaagataa aaagcaaaat atcttagggt attttatttt tataattgaa
       61 agacgtgaat gatatgaaca tgtccttgcg ggtgcttagg aaaaaaatta taagtatgtc
      121 aagtttaaga aaaacttgat tgttggagga ttttttagat gaacgaattt gaagatttgc
      181 taaatagcgt tagtcaagtt gagactggtg atgttgttag tgctgaagta ttgacagttg
      241 atgcgactca agctaacgtt gcaatctctg gaactggtgt tgaaggtgtc ttgactcttc
      301 gcgaattgac aaacgatcgt gatgcagata tcaatgactt tgttaaagta ggagaagtat
      361 tggatgttct tgtacttcgt caagtagttg gtaaagatac tgatacagtt acataccttg
      421 tatctaaaaa acgccttgaa gctcgcaaag catgggacaa acttgttggt cgcgaagaag
      481 aagttgttac tgttaaagga acgcgtgccg ttaaaggtgg actttcagta gaatttgaag
      541 gtgttcgtgg atttatccca gcttcaatgt tggatactcg tttcgtacgt aacgctgagc
      601 gttttgtagg tcaagaattt gatactaaaa tcaaagaagt taacgctaaa gaaaaccgct
      661 tcatcctttc acgtcgtgaa gttgttgaag cagctactgc agcagctcgc gctgaagtat
      721 tcggtaaatt ggctgttggt gatgttgtaa ctggtaaagt tgctcgtatc acaagcttcg
      781 gcgctttcat cgaccttggt ggtgttgacg gattggttca cttgactgaa ttgtcacatg
      841 aacgtaacgt atcaccaaaa tcagttgtaa ctgttggtga agaaattgaa gtgaaaatcc
      901 ttgatcttaa cgaagaagaa ggacgtgtat cactttcact taaagcaaca gtaccaggac
      961 catgggatgg cgttgagcaa aaattggcta aaggtgatgt agtagaagga acagttaaac
     1021 gtttgactga cttcggtgca tttgttgaag tattgccagg tatcgatgga cttgttcacg
     1081 tatcacaaat ttcacacaaa cggattgaaa atccaaaaga agctcttaaa gttggtcaag
     1141 aagttcaagt taaagttctt gaagttaacg cagatgcaga acgcgtgtca ctttctatta
     1201 aagctcttga agaacgtcca gcccaagaag aaggacaaaa agaagaaaaa cgtgctgctc
     1261 gtccacgtcg tccaagacgt caagaaaagc gtgatttcga acttccagaa acacaaacag
     1321 gattttcaat ggctgatttg tttggtgata tcgaacttta atcaaattga aaattcacaa
     1381 aatcctttgt ttactaaaca agggattttt ctggctcttt gtcaactgta gtgggttgaa
     1441 gtcagctaag cttgagaaag gacaaatttc gtcctttctt ttttgatgtt caaagcgata
     1501 aaaatccgtt ttttgaagtt ttcaaagttt cgaaaaccaa aggcattgcg cttgataagt
     1561 ttgatgagat tattggtcgc ttccagtttg gcattagaat agtgtagttg aagggcgttg
     1621 ataatctttt ctttatcttt gaggaaggtt ttaaagacag tctgaaaaat aggatgagcc
     1681 tgcttaagat tgtcctcaat aagtccgaaa aatttctctg gttccttatt ctggaagtga
     1741 aacagcaaga gctgatagag ctgatagtgg tgtttcaagt cttctgaata gctcaaaagc
     1801 ttgtctaaaa tctctttatt ggttaaatgc atacgaaaag taggacgata aaatcgctta
     1861 tcactcagtt tacggctatc ctgttgtatg agcttccagt agcgcttgat agccttgtat
     1921 tcatgggatt ttcgatgaaa ctgattcatg atttgaacac gcacacgact catagcacgg
     1981 ctaagatgtt g
//
LOCUS       DAVMEI010000201         1984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVMEI010000201 DAVMEI010000000
VERSION     DAVMEI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.205"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            223..1926
                     /gene="ilvD"
                     /locus_tag="VYQ24_001896"
     CDS             223..1926
                     /gene="ilvD"
                     /locus_tag="VYQ24_001896"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1968994.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
BASE COUNT          524 a          414 c          483 g          563 t
ORIGIN      
        1 cacttatctg aaagaatttc tgtaaaaatt acagtacgtt atttaaagaa agcggattaa
       61 aaagcatatt ctggtcatca gaaagtgcac taatccgctt tgttgttttt aattttaaat
      121 atttcacatg cgtatagaat tttctgaaaa ttcaagaatt ttattctgtt cattgccttt
      181 aaattgtgct ataatagtaa aaactgaaat gggagggata agatgactga attagataaa
      241 cgtcaccgca gtagcatcta tgacagcatg gttaaatcac ctaaccgtgc tatgcttcgt
      301 gcgactggta tgacagataa ggactttgaa acatcgattg tgggagtgat ttcgacttgg
      361 gcggaaaata caccatgtaa cattcacttg catgatttcg ggaaactggc taaagaaggt
      421 gtcaaatctg caggcgcttg gcctgtacag tttggaacca ttaccgtagc ggacgggatc
      481 gctatgggaa cgcctggtat gcgtttctct ctaacatctc gtgacatcat cgcggactcc
      541 atcgaggcgg ctatgagtgg tcacaacgtg gatgccttcg tcgctatcgg tggctgtgac
      601 aagaacatgc ctggatctat gattgctatt gctaatatgg atatcccagc tattttcgcc
      661 tatggtggaa ctattgcacc gggaaatctt gatggtaaag atatcgactt ggtttctgtc
      721 tttgaaggta tcggaaaatg gaaccacggt gacatgacag ctgaggacgt gaaacgtctt
      781 gaatgtaatg cctgccctgg ccctggtggt tgtggtggta tgtatactgc taataccatg
      841 gcaactgcta tcgaagttct agggatgagt ttgccagggt catcctctca cccagctgaa
      901 tcagctgata agaaagaaga tatcgaagca gcaggacgtg ctgttgttaa gatgttggaa
      961 cttggtctca aaccatcaga tatcttgact cgtgaagcct ttgaagatgc tattactgta
     1021 acgatggctc tcggtggttc tacaaacgcc actcttcact tgctcgccat tgcccatgcc
     1081 gcaaatgttg acttgtcact tgaggacttc aatacgattc aagaacgtgt gcctcacttg
     1141 gccgacttga aaccatctgg tcagtatgtc ttccaagacc tctacgaagt cggtggtgtc
     1201 cctgcggtta tgaagtactt gttggcaaat ggtttccttc acggagatcg catcacatgt
     1261 actggtaaga ctgtagctga aaacttggct gactttgcag acttgactcc aggccaaaaa
     1321 gttatcatgc cacttgaaaa tccaaaacgt gcggatggtc cgcttatcat cttgaacggg
     1381 aaccttgctc ctgacggtgc agttgccaag gtatcaggtg ttaaagtgcg tcgtcacgtt
     1441 gggccagcta aggtctttga ctcagaagaa gatgcgattc aggccgttct gacagatgaa
     1501 atcgttgatg gcgatgtagt cgttgttcgt tttgttggac ctaaaggtgg tcctggtatg
     1561 cctgagatgc tgtcactttc ttcaatgatt gttggtaaag gtcagggaga taaggtggcc
     1621 ctcttgacgg acggacgttt ctctggtggt acttatggtc tggttgttgg acatatcgct
     1681 cctgaagctc aggatggtgg accaattgcc tatctccgta ccggcgatat cgttacggtt
     1741 gaccaagata ccaaagaaat ttccatggcc gtatccgaag aagaacttga aaaacgcaag
     1801 gcagaaacaa ccttgccacc actttacagc cgtggtgtcc tcggtaaata tgcccatatc
     1861 gtatcatctg cttcacgcgg agccgtgaca gacttctgga atatggacaa gtcaggtaaa
     1921 aaataaactc atacttgaaa atctcttcaa atcacgtcaa cgtcgccttg ccgtaggtat
     1981 ggtt
//
LOCUS       DAVMEI010000202         1937 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVMEI010000202 DAVMEI010000000
VERSION     DAVMEI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.206"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            3..587
                     /locus_tag="VYQ24_001897"
     CDS             3..587
                     /locus_tag="VYQ24_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968995.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            591..788
                     /locus_tag="VYQ24_001898"
     CDS             591..788
                     /locus_tag="VYQ24_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968996.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            778..1329
                     /locus_tag="VYQ24_001899"
     CDS             778..1329
                     /locus_tag="VYQ24_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968997.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            1388..1648
                     /locus_tag="VYQ24_001900"
     CDS             1388..1648
                     /locus_tag="VYQ24_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1968998.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
BASE COUNT          626 a          296 c          369 g          646 t
ORIGIN      
        1 acatgaatgt caaaaagata atgtcaattt ttcaatcctt ttatgttgat gtcagtattg
       61 aggaactgac tttgacttta ccaatcagtt ttgtaaaaag gtttgagtat actcaaatga
      121 cttttcataa ggaatcattt ttattgatta aagaaaagag aagggggagt ttgagttcat
      181 ttgttactca ggctcgcact atgggtgaaa aagccaatat ggatgttgtt ttggtgtttt
      241 cgaagttatc agacagtgaa aaaaagcaat tacttcaagc tagagttccg tttgtagact
      301 ttaagggaaa cctcttcttc cctccattgg gactagtact caatgcgaat gatactgaaa
      361 tccctaagga attaacacct agcgaacaat taacgtggat tgccttttta ttgccagatg
      421 gcgatataaa gcagataaaa tctgtttcta accttctata tggtggtgct tatgctttgt
      481 cgcattcaac ttttttagct gaaacggatg aaaatactag ctatgtcata tggcagagaa
      541 aattcaatca gttatccttg ccactttctc agcatgtttt aaaatgaaag atgctagaga
      601 tatggaaata tcgtcctttt gtatctgagt tttggaatga ttttaaaaat aatcatgata
      661 aacaatttgt agatccgatt tctctttatt tgaccttaaa agatgatgat gacccacgta
      721 tagaggaaga gagtgaagca ctagaaaata tgatattaca gtatctggga gaagatgatg
      781 ccagctaata cgaaagttat ttttcaagaa atgtttgcgg attttcagaa ctattatgtt
      841 ctgattgggg gaactgctac ctctatcgta ttggattcgc aaggatttaa aagtcgcaca
      901 acaaaagatt atgatatggt catcatttat gaagtaaaaa ataaggaatt ttatactacc
      961 ttgaatcatt ttttagaatt gggagagtat caaggaagtc agaaagatga gaaagcgcag
     1021 ctttttcgat ttacaacaac taatcctgag tttccttcta tgattgaact atttagtatc
     1081 ttaccagaat atccattaaa gaaggacggt cgagaaattc ccttacattt tgaccaagat
     1141 gctagtttat cagccttatt attggatgaa gattattata atatattggt gcatgaaaaa
     1201 gaaaccattc aggggtattc ggtattgagt aattgtggtt tatactcttc gaaaatctct
     1261 tcaaaccacg tcagcttcca tctacaacct caaaacagtg ttttgagcag cctgcagcta
     1321 gcttcctagt ttgctctttg attttcattg agtattaatt atttttaagg ctaaagcttg
     1381 gctggatatg agggaacgct ctgctacagg tgctcaaggt ttaagtaagt ccattaaaaa
     1441 gcatttgaat gacattaccc gtttgacagc ttccttgcta ggagatgaaa agttatcggc
     1501 tataacatca agtagtgcgg taaaagcaga catacaccgc tttgtgatag aattagagcc
     1561 tgtgaagtca actattcttc aaaatgatga catttcattg gatcaaaatg aaatttttga
     1621 aattctgaaa aattttctcg atggttaaaa taattgtagc gagatggcta tattgaattc
     1681 gtctatatct ggaaactaga aaaaacttca atttcaggag aaaatgaagt caatcttccc
     1741 acaatcaaac gtatagtatc aaggtttttc aagacctgat attatgcgtt ttttgctttt
     1801 caaaactttt tgcctagtct ttgtttttat cctctagtca cttgatttgt ttcaggtggt
     1861 tttttagtat agtgaaatga aacgagaaca ggacaaattg atcaggacag tcaaatcgat
     1921 ttctaacaat gttttag
//
LOCUS       DAVMEI010000203         1931 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVMEI010000203 DAVMEI010000000
VERSION     DAVMEI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.207"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>1931
                     /gene="ftsH"
                     /locus_tag="VYQ24_001901"
     CDS             <1..>1931
                     /gene="ftsH"
                     /locus_tag="VYQ24_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1968999.1"
                     /translation="IKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYTELVQEITD
                     GNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTILPADTTVS
                     ELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGGGNGRNPMS
                     FGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGARIPAGVLLE
                     GPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAAPAIIF
                     IDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNRSDVLDPAL
                     LRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFVGADLENVL
                     NEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHEAGHTIVGL
                     VLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGRVAEEIIFN
                     VQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKSISEQTAYE
                     IDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYETGKMPEAV
                     EEESHALSYDEVKSKMNDE"
BASE COUNT          625 a          325 c          437 g          544 t
ORIGIN      
        1 aattaaaaat ccttttctat ggttattatt tatctttttc cttgtgacag gattccagta
       61 tttctattct gggaataact caggaggaag tcagcaaatc aactatactg agttggtaca
      121 agaaattacc gatggtaatg taaaagaatt aacttaccaa ccaaatggta gtgttatcga
      181 agtttctggt gtctataaaa atcctaaaac aagtaaagaa ggaacaggta ttcagttttt
      241 cacgccatct gttactaagg tagagaaatt taccagcact attcttcctg cagatactac
      301 cgtatcagaa ttgcaaaaac ttgctactga ccataaagca gaagtaactg ttaagcatga
      361 aagttcaagt ggtatatgga ttaatctact cgtatccatt gtgccatttg gaattctatt
      421 cttcttccta ttctctatga tgggaaatat gggaggaggc aatggccgta atccaatgag
      481 ttttggacgt agtaaggcta aagcagcaaa taaagaagat attaaagtaa gattttcaga
      541 tgttgctgga gctgaagaag aaaaacaaga actagttgaa gttgttgagt tcttaaaaga
      601 tccaaaacga ttcacaaaac ttggagcccg tattccagca ggtgttcttt tggagggacc
      661 tccggggaca ggtaaaactt tgcttgctaa ggcagtcgct ggagaagcag gtgttccatt
      721 ctttagtatc tcaggttctg actttgtaga aatgtttgtc ggagttggag ctagtcgtgt
      781 tcgctctctt tttgaggatg ccaaaaaagc agcaccagct atcatcttta tcgatgaaat
      841 tgatgctgtt ggacgtcaac gtggagtcgg tctcggcgga ggtaatgacg aacgtgaaca
      901 aaccttgaac caacttttga ttgagatgga tggttttgag ggaaatgaag ggattatcgt
      961 catcgctgcg acaaaccgtt cagatgtact tgatcctgcc cttttgcgtc caggacgttt
     1021 tgatagaaaa gtattggttg gccgtcctga tgttaaaggt cgtgaagcaa tcttgaaagt
     1081 tcacgctaag aacaagcctt tagcagaaga tgttgatttg aaattagtgg ctcaacaaac
     1141 tccaggcttt gttggtgctg atttagagaa tgtcttgaat gaagcagctt tagttgctgc
     1201 tcgtcgcaat aaatcgataa ttgatgcttc agatattgat gaagcagaag atagagttat
     1261 tgctggacct tctaagaaag ataagacagt ttcacaaaaa gaacgagaat tggttgctta
     1321 ccatgaggca ggacatacca ttgttggtct agtcttgtcg aatgctcgcg ttgtccataa
     1381 ggttacaatt gtaccacgcg gccgtgcagg cggatacatg attgcacttc ctaaagagga
     1441 tcaaatgctt ctatctaaag aagatatgaa agagcaattg gctggcttaa tgggtggacg
     1501 tgtagctgaa gaaattatct ttaatgtcca aactacagga gcttcaaacg actttgaaca
     1561 agcgacacaa atggcacgtg caatggttac agagtacggt atgagtgaaa aacttggccc
     1621 agtacaatat gaaggaaacc atgctatgct tggtgcacag agtcctcaaa aatcaatttc
     1681 agaacaaaca gcttatgaaa ttgatgaaga ggttcgttca ttattaaatg aggcacgaaa
     1741 taaagctgct gaaattattc agtcaaatcg tgaaactcac aagttaattg cagaagcatt
     1801 attgaaatac gaaacattgg atagtacaca aattaaagct ctttacgaaa caggaaagat
     1861 gcctgaagca gtagaagagg aatctcatgc actatcctat gatgaagtaa agtcaaaaat
     1921 gaatgacgaa a
//
LOCUS       DAVMEI010000204         1885 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVMEI010000204 DAVMEI010000000
VERSION     DAVMEI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.208"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1223..1462
                     /locus_tag="VYQ24_001902"
     CDS             1223..1462
                     /locus_tag="VYQ24_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969000.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            1447..1725
                     /locus_tag="VYQ24_001903"
     CDS             1447..1725
                     /locus_tag="VYQ24_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969001.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
BASE COUNT          627 a          244 c          353 g          661 t
ORIGIN      
        1 tatttgtttt cggttttact tcttttttct aatttttcag ttccatttat caaagcaaag
       61 gaatacgaat tatttaaaaa tagcaagtaa tagaaacata ggttaaatct tagaacggct
      121 taaaatcaga tatgataaag ggaaatagtc ttatctaggt aactaagtgc tacaaacaca
      181 gtcgaggatc acttgttagg attacatttt aatcatgctt aataagaatg gagaattcct
      241 atggggattg tattactctg aagtttcgaa taccttagtt ctcgtgattt tgagctgttc
      301 tagctttaga atccttcaga atgtaaaatt ttaagcagtc aaagacttag aatagcctgg
      361 aaacatttat tttatagcaa ttctaggctt agaattgctc atatatatgt atttgagctg
      421 ctcgatagtt aggacagctc tattctgaga aatctgagct atcaatgctt cagaatagta
      481 tttcgcaaat ggtttttatt gaagaggtga ttgttttatg atagtctaaa ataaaactga
      541 tttaatggag taaatggaga atcgtcacat gaaacatttt tttgcaggaa ttggtgagat
      601 aaaacttgtt ggctacttat tgtatatatg tgtaggacta gcccttcttt ttcatatata
      661 tgtcataggt tcggaactga gttttgtgaa aatagtgtta tctattttgg gagttatatt
      721 tgtaagtatg ttaactattg ctcgaattta tcggactttt ctataaagaa tagtttggac
      781 ttgaactgta tctaaaaaca gaggttggtc gattatcagc ctctttcttg tcgttgaaaa
      841 gagtgagata tattgacttt tgaaaaaaat gtcataattg agttgggata aacctaaaag
      901 taatatagta gattgaatct ggaatagtcc gttatgactg ttaaaatatt tctagaaatt
      961 aatttgactt tcctaatcga tttgttcaca tcttgtttca atctactata ctttctcgtt
     1021 aaaccgttcg gcaattcatg gtgacatgga cacatagtag taagcacact atgtggcttg
     1081 gcagagctaa aaactgttcc cttgcgataa gcctaataag cacaacatag ggagttagag
     1141 aagccgactc taatcaacca ctttgggcag tagtgagaac tgccccgtgc ttttttattt
     1201 tgagaaaata tggagtttgt cgttgaaatt acttgattgt attttggact atcaagaaag
     1261 attcaatgga aaaacatgtc aagtatcaac gaattataag tatttagaga ttttcaaagt
     1321 gaatttttgc ttgactgatt tacatcattt atttgactta tacaaaatca cacgagatta
     1381 tgctagtcaa acaaaacctg ctattcaagc tggtgttttt attttagaag attttagaaa
     1441 tatcctatgt acaatgatgt aatcgaaagg atatctttat atgagtttat cggtgatatt
     1501 ttctattcta aaataataag ttgttgcatc gtagctaggg atttatctaa aaatactatg
     1561 aaattggacg tcatattttt tgaggataaa aataaaagat ccgcagtttt aggtttacga
     1621 agagacaaaa gcggagtatt taaatcagtt actctacatt ttacaagcgc taagaaatat
     1681 gctaaagttc gtaaaacaga tgtgaaagaa atgaaatggc tataaagatt gtccgcaggc
     1741 ttaagcttgc gttttttgtt gtttaacgaa attaaaaaac aagctctctc atcggaaatg
     1801 tttttatagg aatgaaggaa gatgatgcta tgtttaaaaa gagaatcgaa aagggcaaaa
     1861 gtagtatctt tatctatttt ttgga
//
LOCUS       DAVMEI010000205         1861 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVMEI010000205 DAVMEI010000000
VERSION     DAVMEI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.209"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            54..1178
                     /locus_tag="VYQ24_001904"
     CDS             54..1178
                     /locus_tag="VYQ24_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1969002.1"
                     /translation="MGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNFLSVTFSSIT
                     GDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYFYPFLKKVFFALLLLH
                     IVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQK
                     HLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLR
                     SDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMS
                     VLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFALGFSTKGRN
                     RGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            1175..>1861
                     /locus_tag="VYQ24_001905"
     CDS             1175..>1861
                     /locus_tag="VYQ24_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1969003.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHR"
BASE COUNT          629 a          259 c          344 g          629 t
ORIGIN      
        1 ccttttatct tctatattta gataaaatag ggattgaaag atttttattt cctttgggct
       61 tatattccta ttttcgatgg atgaaacagt atgagaggga tagaggacta ttcctaagtt
      121 tactactatc tcttttatat gagagcactc ataactttct gtccgtaact ttctcctcta
      181 taacaggaga taattttgtt ttacaatatc atttcccatt ctttttcgtt gtgacggtgt
      241 taacctattt tgttacatta aaaatcattt attatttcca tttggaacta gcctattttg
      301 acaaggacta cttttatcct ttcttgaaaa aagtattttt tgctttacta ttgctacata
      361 ttgtatcttt cgtttcagat atggtaagta cgattaaaca tttgaatagt tttggaagta
      421 ttttgtcatc tattgtcttt atctctctcc ttttgacctt ctttgcaatg aattctcata
      481 aagttcaaat ggagaaagag attgctttga agcagaagaa atttgaacag aaacatttac
      541 agaattacac agatgaaatt gttggtctgt ataatgaaat ccgtggtttt cgacatgatt
      601 atgctggaat gcttgtcagc atgcagatgg caattgacag tggtaattta caggaaattg
      661 acagaattta caatgaagtt ttagtcaaag caaatcataa attgcgttca gataagtaca
      721 cttactttga tttgaacaac atagaagact cagctttacg aagtttggtt gctcagtcaa
      781 ttgtctatgc tcgaaataat ggtgtagagt ttacactgga agtaaaagat acgattacca
      841 agcttccaat tgaactattg gatttggttc gtatcatgag cgttttattg aataatgctg
      901 tcgaaggatc ggctgatagc tataaaaagc agatggaagt agcagttatt aagatggaaa
      961 ctgaaacagt tatggtgatt cagaattcat gtaaaatgac gatgactcct tcaggagatc
     1021 tatttgcctt aggattctcc actaagggaa gaaatcgcgg agtcggatta aataatgtga
     1081 aagaactact agataagtac aacaatatta ttttagaaac agagatggaa ggcagtacat
     1141 ttagacaaat cattagattt aagagggaat ttgaatgaaa gttttaattt tagaagatgt
     1201 tattgaacat caagtgagac tagagagaat attggatgaa atttcgaaag aatcgaatat
     1261 tccaatatca tacaagacaa cgggaaaagt ccgtgaattt gaagaataca ttgaaaatga
     1321 tgaagtaaat cagctttatt tcctagatat cgatattcat ggaattgaga aaaagggatt
     1381 tgaagtggct cagctcattc gtcattacaa tccttacgct attattgtct ttatcactag
     1441 tcgatcagag tttgcgactc taacctataa ataccaggta tcagccctag attttgttga
     1501 taaggatatc aatgatgaga tgtttaagaa gagaattgag caaaatatct tctacacgaa
     1561 gagtatgtta cttgaaaatg aagatgttgt agattatttc gactacaatt acaagggaaa
     1621 tgatttaaaa attccttacc atgatatttt gtatattgaa acaacagggg tatctcataa
     1681 attgcgcatt attggtaaga attttgcaaa agagttttat ggtaccatga cagatattca
     1741 ggaaaaggac aaacatactc agcgatttta ttctcctcac aagtcatttt tggtaaatat
     1801 aggcaatatc agagaaattg atcgaaaaaa cttagaaatt gttttctatg aagaccatcg
     1861 t
//
LOCUS       DAVMEI010000206         1859 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVMEI010000206 DAVMEI010000000
VERSION     DAVMEI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.210"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(127..1758)
                     /locus_tag="VYQ24_001906"
     CDS             complement(127..1758)
                     /locus_tag="VYQ24_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1969004.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          568 a          366 c          391 g          534 t
ORIGIN      
        1 agaggtgtac tattctagtt tcaatatact atatatctgc agaatactct ttcagtttca
       61 ttcagcctag tcttgcttag gaaaaagtaa aaagccatcc aattatgatg gatagcttct
      121 tggttcttag atttgttctg caaaaacctt ttcagcaagg ttcatagcgt ggtctgatac
      181 acgagtgtag tgtgagatga tgtcgataaa gttgacccca gcttgtgttg aacattcgcc
      241 tttgttgagg cgtttgatgt gggtttttct gagaacacgt tctatcttat tgattgcttc
      301 atgacgttct acaagactgc gtgctttttc aatatcattg ttttccacac tatccagagc
      361 atctttgata aagtcactag tttgacggta aacttcctct aattctttca aggcggcatc
      421 agaaaattca acatttttcc gttgaagata gtcagtcaga ttgagtagag cctccgtgtg
      481 gtctccaatc cgttccaaat cacgggagga atcaaggata ttggtaagca cttcactttc
      541 tttttggctg agagcttcgc ttgaaagggc aatgagataa cgtgttaatt gctcatcgat
      601 ggtgttaatt gcttcttcgg ttttatgccc tttttcagca actttttcat ccaagtcaat
      661 gatgtactta taggaaaggt caaaggcttt agcagcgtag tttcctaagt gcaagagctc
      721 ttttttagca tttcctagag cgatagatgg ggcttgtttg atgaaatgtt catcaagata
      781 taagggttcg tatttgacaa cctcgtcctc tccaggaata atcttggtta caaagtaagc
      841 cagagctccg ataaatggaa attggacaat ggtgttggta atattaaagg ttccgtgagc
      901 aaaggcgatg gtcatttccg gtgctagatt tagcgtagct tcaaaccaat gaatcaggac
      961 agtaaaagga actagaaaaa taacgcagac gactgttccg ataacgttga aggcaacatg
     1021 agctcctgct acccgtttag ctgcaatatt agcccctaaa gaggcaatga tggctgtaat
     1081 ggttgtcccg atattgtcac caaatagaac tggcaaagct ccctgtaggt caattagatt
     1141 gccggcgtag aggttttgta aaatcccaat ggtagccgaa gaagcttgaa ttagcaaggt
     1201 caagccggta ccgacaaaga cacccaaaac aggattctta cttagctcaa tcatatagtc
     1261 cttaaagacc tgtaaatcct tgagtggagc cattgcgccg ctcatgagat tgagggcaaa
     1321 aaagatacca ccgacaccaa agaggatgcg tccgatatta ttgactgtcc gatttttcgt
     1381 aaaaaagaga cagacggcac cgataaagag cataggtagg gcatagttac ctaatttaaa
     1441 accgatgaga aaggatgtga cagttgtccc aatattagca cccatgacaa tcccgatagc
     1501 ctgacgtaag gttaagagac cggcactgac caggccgact gtaataactg ttacaccaga
     1561 actagactga attagagcag tcatcccaat accaaccaga actccaaaga aaggattact
     1621 agtatatttg tcaatgtaaa aacgaaggcg atctccagca gcttgttgca aaccgtctcc
     1681 catggtcttg atgctatata agaatagccc cagaccacct aaaaagtgaa ataaaatttc
     1741 ctgccaatta atggacattt ctttttcctc cgaaaaataa tcgcggaata tctcctattc
     1801 tattttaaag gataaaagta aatctaacaa gtgttaatgt caagtttgta aaagaaaat
//
LOCUS       DAVMEI010000207         1826 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVMEI010000207 DAVMEI010000000
VERSION     DAVMEI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.211"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(168..932)
                     /locus_tag="VYQ24_001907"
     CDS             complement(168..932)
                     /locus_tag="VYQ24_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1969005.1"
                     /translation="MFKLSFQSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TSDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            complement(925..1695)
                     /locus_tag="VYQ24_001908"
     CDS             complement(925..1695)
                     /locus_tag="VYQ24_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1969006.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGEGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFLGLTGTEKEDLHV"
BASE COUNT          630 a          402 c          297 g          497 t
ORIGIN      
        1 ttgattaggg aatttaaaac aatttctaac aatattttaa aaactgaacc gtactatttc
       61 aacttccata aattacaata ttgataggca cagttcccat acctgtccaa attcgaataa
      121 cgtttagtat aaattagaat atgactacaa catcacaaat aattagacta tctataatat
      181 ctacgatgta acatatacat aggaagcacc gttatcaaga gcgccaaggc tatcaaccag
      241 ataagatgaa gatacaaacc tgtcacctca cctccccata taatacgaat catgcgggta
      301 gcgtgataca agggagaaaa ccaagtcaaa tcaccaagta ttgggggcaa ggaagtaatg
      361 ggaaagaagg tatctgaaaa catataaaga ggacggatta ccaaaacatt gtagtaattc
      421 agaaattccc tagacggtgc caagtaggta aagattagtc ctaaaacacc aaacaaataa
      481 cccactataa ataaaatcag aggaatcaat aacatcaaga catgaaaatg cgctgtgata
      541 agcatggaaa tcagccaaaa aatcccaccg aaaatcactg cacgaatacc tgcccagata
      601 gcctgaccca ccacaatatc actcgtattg attggcgtca tcaacatagc atcataagtc
      661 ttttcaatct tatactggat aaaggcattg accgttgttt ctgcggtggc cgcagacatg
      721 gcagtcgcag caattaaacc tgtaatgaca aaatcacggt agagcatacc gttaacatgc
      781 gtcaagtagg caccaactcc aaatcccatt gctaaaagat aaaagagggg gtcgataagg
      841 ttggggacaa ttgaaatttt aaataaattt ctaaaactca tgtagttgcg taccacaacg
      901 tgtccgatgg actgaaatga taacttaaac atgcaagtcc tccttctctg tcccagtcaa
      961 ccccaaaaaa agcgtctcaa ggttgtcagc atcatacttg atgagcaagt cttctacagt
     1021 tccttcttct ttgatagtcc cttggtggag catcagcacc ctatctgtca aatattcaat
     1081 ctcattcata tagtgggaag taataatgat agaaacacct aattccttca actccaaaag
     1141 tttatcccaa ataatattgc gggattgaat gtctaaacca actgtcggtt catctaaaat
     1201 gataatatca ggccgattga tgagagctct tgccaagaca agacgtcgtc gcatcccacc
     1261 cgacagctga ttcaccatct tttgcgcatg tttgctcagt tgaacaaaat ccaagagaga
     1321 atcgattctc ttcctaagtt cgacagatga gataccataa catagaccgt gagcaaaaag
     1381 attatcataa acattcaagg tcatatccag catatcctcc tgccctacaa tacccagaca
     1441 agatttactc ttacgacgat tggtttcaat attttgcccc aagatatcaa tctctcctga
     1501 ggaaattgag gtcagtgagt aaatcatctt taaagtggat gttttgccag caccgttgat
     1561 tcccaagata cccaaacact ctccttctcc caattcaaag ctaattccct tgacagcttc
     1621 ataagaacca tattgctttt ttagattttt aacacttaac ttcttcactc tttctttccc
     1681 ccacaaatag agcatgttcg atactttgtt aattcatctc acttttgaaa aaatgttgag
     1741 aatgtaatct taagattaac gtccttcaac tgtggatact gctgcaatta cttttgtaat
     1801 tttacctcca tttaaactca gacaat
//
LOCUS       DAVMEI010000208         1824 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVMEI010000208 DAVMEI010000000
VERSION     DAVMEI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.212"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..697)
                     /locus_tag="VYQ24_001909"
     CDS             complement(<1..697)
                     /locus_tag="VYQ24_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1969007.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKY"
     gene            complement(753..1775)
                     /locus_tag="VYQ24_001910"
     CDS             complement(753..1775)
                     /locus_tag="VYQ24_001910"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1969008.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
BASE COUNT          522 a          398 c          311 g          593 t
ORIGIN      
        1 tgtatttttc tagttctttt tggtatttgc tattggattc caatttttct ttttgccatt
       61 ttttaaaaac ctcgttatat tcttttgttg taacaatatc tttttgcaat ttcattcctt
      121 taaagatata tggttccccc ttaataccaa cttgtgagta tggttttgag aatggtacta
      181 cgttacttac aactggagaa ccaccagatg aagctgttgg catcaataat gaactatctg
      241 tcgaccaagc ttgagctttg gcatattttt catatctctt ctctaggtca gtggtctcag
      301 aaacagcatc ttctaacaat ttcttatatt tatccaaacc aggtttagct acaacatcct
      361 tatcttttcc tttcgtaata ccaaggtgtt tcatggcaga accagatttt ggatccataa
      421 tattcaagta agacgctgga tcttgatagc ttggagccca tcctgtacta ttcaaatcat
      481 agtctttttg agaaggagca acattgccgt atttatcatt ttccatcaaa ccatcaataa
      541 catttccaat aacgtctgtc ctcgatgttc gagtcgctat actgtagccc aatgatgctg
      601 gatctactgc atagacataa gaaaatgttg tcggtgcatc tgcttcttta tcagtttttc
      661 cacaagccac taaaatagct gacgtgctca ggaccactcc tgctgttaag agccactttt
      721 tctatttcat aaaaaatctc ctttggttta ttttaatcta cttttacaat ccaaccttct
      781 ggcgcttcaa tatcgccaaa ctgaataccc gtcaattcat tatataattt acgcgtcaca
      841 ggacctactt ctgtttcact atagaataca tggaaatcat caccatgttg aatacctcca
      901 attggagaaa taaccgctgc tgtaccacag gcacctgcct ctacaaaacg gtcaagatta
      961 tcaattggaa catcaccctc aataggagtt aatcccaagc gatgttctgc caaataaagc
     1021 aaggaatact tggtaataga tggcaagata gatggactca atggtgttac aaattcatta
     1081 tcagctgtaa ttccaaagaa gttagctgat ccgacttctt caatctttgt atgagttgat
     1141 gggtccagat agataacatc tgagaaatga cgtgacttgg ccatttttcc tggtaagaga
     1201 cttgcagcat agtttccacc aaccttagcc gcacctgtac catttggtgc tgcacggtcg
     1261 tactcatcct gaatcaagaa gttggttggg accaaaccac ctttaaagta atttccaact
     1321 ggcatagcaa agatggtgaa aatgtactct tctgccggtt ttaccccgat aatatctccg
     1381 acaccaatca aaagagggcg aagatataag gttccacctg ttccgtatgg tggtacgtat
     1441 tcttcattcg cacggacaac tgctttacaa gcttctacaa acatgtctgt cggaacttgt
     1501 ggcatcaaga gacggtcaca tgtacgttgc agacgtttag cattttcatc aggacggaac
     1561 agttgaacac tgccatcctt agtacgataa gctttcaaac cttcaaatgc ttgttgtcca
     1621 tagtgaagac ttggagaaga ctctgaaata tgcaaagttg catcctctgt aagctctcct
     1681 tgatcccatt gtccattttt gaaatgagca agatagcgat aaggtaattt catataggaa
     1741 aaaccgaggt tttcccaatc aatcgttact gtcatattcc actccttatt ctaaaaacct
     1801 atttcttata ttctacacta tttt
//
LOCUS       DAVMEI010000209         1735 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVMEI010000209 DAVMEI010000000
VERSION     DAVMEI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.213"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..617
                     /locus_tag="VYQ24_001911"
     CDS             27..617
                     /locus_tag="VYQ24_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1969009.1"
                     /translation="MGVFGVIYFAFMFGVGMMGLIPILFLIYPTVLAIVAGTVVMLFM
                     AKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRKIGNYNSFKYNMLS
                     YAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYPHMALVALGAFLGG
                     ILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            518..1075
                     /locus_tag="VYQ24_001912"
     CDS             518..1075
                     /locus_tag="VYQ24_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1969010.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVVGLIVGGLIWLK"
     gene            1063..>1735
                     /locus_tag="VYQ24_001913"
     CDS             1063..>1735
                     /locus_tag="VYQ24_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1969011.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRS"
BASE COUNT          591 a          220 c          336 g          588 t
ORIGIN      
        1 aattaaaagt aaaagattta gtaagcatcg gtgtttttgg cgtaatttat tttgccttca
       61 tgtttggagt tggtatgatg ggcttgattc caatattgtt cttaatatac ccgacagtat
      121 tagccatagt tgcaggaact gttgttatgt tatttatggc taaggttcaa aagccatggg
      181 cactatttat atttggtatg atatcaccac ttgtgatgtt tgcagctggt catacctacg
      241 tagttgtggt tttatcactt atagtaatga taatagcaga attaattaga aagattggta
      301 attataattc atttaaatac aatatgcttt cttatgcaat cttcagcaca tggatatgta
      361 gctctttaat gcaaatgctt ttagcaaaag aaaaatatat ggagtggtct ttgatgacga
      421 tgggaaaaga ttatgttgat gtattagaaa agttaataac ttatcctcac atggctttag
      481 tagccttagg tgctttctta ggaggaattc ttggagcata tataggcaag gctctattga
      541 aaaaacactt ttcaaatgga ttatattgtg tgggatactt tactccttgc ctaattttat
      601 ggtgttatct gaattaaacc ctatagttaa gatgtttttg agtataccta ttgttattag
      661 aatgtttatt ttaccattta tggcagcaag ctttatgata aagacctcgg atgtaggcgc
      721 aataatttca tcgatggata agcttaagat ttcaaagaat gtatccatac ctattgcggt
      781 tatgtttaga ttcttcccat cttttaagga ggagaagaaa aacatcaaaa tggctatgag
      841 agtaagaggg ataaatttta aaaacccagt caaatatctt gaatatgttt ctgtgccact
      901 actcattata tcatctaata tatcagatga cattgcaaaa gcggcagaaa caaaggcaat
      961 agaaaatcca attgccaaga ccagatacat tcgcgtaaag atacagctaa ttgattttgt
     1021 ttatgtttta gcggttgttg gacttattgt gggaggctta atatggttga aataaaaaat
     1081 ttaagtcttg attatggtga agagcatata ttagatgata tatcactatc catagccgag
     1141 ggagagtgcg tgctatttac aggaaaaagt ggaaatggta agtcatcttt aataaattca
     1201 atcaatggac tagctgtaag gtatgacaac gcaaagacaa agggcgaaat cattattgat
     1261 ggtaagaata taaaaaattt ggaactttat caaatctcaa tgcttgtttc aactgttttt
     1321 caaaatccta agacatattt ttttaatgtc aatacgacat tagaattatt attttatttg
     1381 gaaaatatcg gtcttgcaag agaagagatg gacaggcgtt tgaaggatat gcttaagata
     1441 ttcccgataa aaaatctttt gaacagaaat atatttaatc tatccggcgg tgaaaaacaa
     1501 attctttgca ttgcagcttc ttatatagca ggtacaaaga ttatagttat ggatgagcct
     1561 tcatcgaatt tagatattaa aagcataagt gttttggcaa agatgctaaa gatattaaaa
     1621 gagaaaggta taagcataat tgttgcagag catagaattt attatttgat ggacatagtt
     1681 gaccgtgtat ttttaataga taaaggaaag cttaaaaaaa cttatactag aagtg
//
LOCUS       DAVMEI010000210         1732 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVMEI010000210 DAVMEI010000000
VERSION     DAVMEI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.214"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..1560
                     /locus_tag="VYQ24_001914"
     rRNA            <1..1560
                     /locus_tag="VYQ24_001914"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1637..>1732
                     /gene="rrf"
                     /locus_tag="VYQ24_001915"
     rRNA            1637..>1732
                     /gene="rrf"
                     /locus_tag="VYQ24_001915"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          474 a          358 c          510 g          390 t
ORIGIN      
        1 aggggaaggc tcgtccgccc tgggttagtc gggacctaag gagagaccga aaggtgtatc
       61 cgatggacaa caggttgata ttcctgtact agagtatgta gtgatggagg gacgcagtag
      121 gctaactaaa gcagacgatt ggaagagtct gtctaagcag tgaggtgtga attgagtcaa
      181 atgcttaatt ctataacatt gagctgtgat ggggagcgaa gtttagtagc gaagttagtg
      241 acgtcacact gccaagaaaa gcttctagcg tttaaacata ctctacccgt accgcaaacc
      301 gacacaggta gtcgaggcga gtagcctcag gtgagcgaga gaactctcgt taaggaactc
      361 ggcaaaatga ccccgtaact tcgggagaag gggtgctgac ttaaagtcag ccgcagtgaa
      421 taggcccaag caactgttta tcaaaaacac agctctctgc taaatcgtaa gatgatgtat
      481 agggggtgac gcctgcccgg tgctggaagg ttaagaggag tgcttagcgt aagcgaaggt
      541 atgaattgaa gccccagtaa acggcggccg taactataac ggtcctaagg tagcgaaatt
      601 ccttgtcggg taagttccga cccgcacgaa aggcgtaatg atttgggcac tgtctcaacg
      661 agagactcgg tgaaatttta gtacctgtga agatgcaggt tacccgcgac aggacggaaa
      721 gaccccatgg agctttactg cagtttgata ttgagtgtct gtaccacatg tacaggatag
      781 gtaggagtct aagagatcgg gacgccagtt tcgaaggaga cgctgttggg atactaccct
      841 tgtgttatgg ccactctaac ccagataggt gatccctatc ggagacagtg tctgacgggc
      901 agtttgactg gggcggtcgc ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga
      961 atggttggaa atcattcgca gagtgtaaag gtataaggga gcttgactgc gagagctaca
     1021 actcgagcag ggacgaaagt cgggcttagt gatccggtgg ttccgtatgg aagggccatc
     1081 gctcaacgga taaaagctac cctggggata acaggcttat ctcccccaag agttcacatc
     1141 gacggggagg tttggcacct cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc
     1201 aagggttggg ctgttcgccc attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga
     1261 cagttcggtc cctatccgtc gcgggcgtag gaaatttgag aggatctgct cctagtacga
     1321 gaggaccaga gtggacttac cgctggtgta ccagttgtct tgccaaaggc atcgctgggt
     1381 agctatgtag ggaagggata aacgctgaaa gcatctaagt gtgaaaccca cctcaagatg
     1441 agatttccca tgattatata tcagtaagag ccctgagaga tgatcaggta gataggttag
     1501 aagtggaagt gtggcgacac atgtagcgga ctaatactaa tagctcgagg acttatccaa
     1561 agtaactgag aatatgaaag cgaacggttt tcttaagttg aatagatatt caattttgag
     1621 taggtattac tcagagttaa gtgacgatag cctaggagat acacctgtac ccatgccgaa
     1681 cacagaagtt aagccctaga acgccggaag tagttggggg ttgccccctg tg
//
LOCUS       DAVMEI010000211         1729 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVMEI010000211 DAVMEI010000000
VERSION     DAVMEI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.215"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..1624)
                     /gene="pfbA"
                     /locus_tag="VYQ24_001916"
     CDS             complement(<1..1624)
                     /gene="pfbA"
                     /locus_tag="VYQ24_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1969012.1"
                     /translation="MKYFVPNKVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHTIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKVIRVAKDSAEYLGKVSDITVTKNVINNNSKETEQPNI
                     ELLRVSDNLVVSENSIFGGKEGIVIEDSKGKIT"
BASE COUNT          526 a          330 c          221 g          652 t
ORIGIN      
        1 cggttatttt accctttgaa tcctcaataa caattccttc tttaccaccg aatatactat
       61 tctctgagac tactaaatta tcactaactc gtaataattc aatatttggt tgttctgttt
      121 ccttagaatt attattaatt acattttttg ttacagtaat atctgatact tttcctaaat
      181 attctgcact atcttttgca actcgtatca cttttgtttt aggatcggca atattaaata
      241 tattttcggc gataaccacc tgtttattta aatctaatag gtctttagtg tttttatagc
      301 tataagcatt tactaaagct gctccgtttt ctcgataatg tacactctct cctttaactt
      361 tcttatcaaa gcgatttcct ttgattaata catcagtgaa tcctgtaaaa cgtacacctg
      421 catacatcat gttatcaaaa tgattattta aaattttaat attagagggg ttctgtgtcg
      481 acaatgtttg atagtgtgtg ccaattgctg ttactaattc cccagattta tcacttttgc
      541 caaaatagga attttgaata gtcacatttt cagatttttt cccatcatca ttcaaggcat
      601 aaggaaaacc ttttctagtt aatggttcaa tctgaatgct ctccttactt atgatttgcc
      661 catccttcat cgttttgggt aaggcttgcc caagaaaacg agaattatca actaatacat
      721 ttttcgaacc tgcaatttga atagtatgcc cttgataact atccttgaat gttacatttt
      781 ttatagttac gttatttgaa ttcccaatag caaatgcacc tgaagaattt ataagtggta
      841 gattttttgc tttagttcct tcttcattca aagcaccgtt catatcaatc gtaccaccag
      901 aataactaat atcttctgtt gggccccatt ctacttgcgc accatcatct gtaaataaac
      961 ctgtcataaa aacaatggaa gggtgattct taatatttat accatttaga attgtagctt
     1021 tctcattcaa ttctaagtgt gtatgacttt ttaaaaaaac aatttctttt actaaataag
     1081 ttccttcagg aaaatataca tttcctccac ctagcccttg agctgcagca tctattgcat
     1141 cttgaattgc ttgtcgatca tcatgaatcc catcacctac cgcaccataa tcttttacac
     1201 ttaaagcagt atcttcaaat tcttttttga taattctgtc acttataaca tcttccttat
     1261 aactcttatt ttcattagta gatgttactg tagattcaac tgttctgttg tctaattgtg
     1321 aattggaacg aatagtacca ttagagctga tactattaac acttaacgaa ttatctaaac
     1381 cattagtggt tgaattagag gcaatcattt cagcagaacg attttgatta acagttggtg
     1441 aattatctaa atcattagta attgcattag aagactcttt tgtagccatc ggtgaagaac
     1501 tagtaacaac ttcatccgat aaaatacctt ggcttcccaa aattgaaatt gccaatagta
     1561 ccgagcaagt ccccaccttt aatttacgaa tactgaatac cttattagga acaaaatatt
     1621 tcatcagaac ctccaatttt tttactattt ttaacaattg attattactt ttcttaaaat
     1681 agatagaatg acaccgctgt cattatcttt atcatatatt ttccaaatt
//
LOCUS       DAVMEI010000212         1720 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.216, whole genome shotgun sequence.
ACCESSION   DAVMEI010000212 DAVMEI010000000
VERSION     DAVMEI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.216"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            129..1622
                     /locus_tag="VYQ24_001917"
     CDS             129..1622
                     /locus_tag="VYQ24_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1969013.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKVISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>1720
                     /locus_tag="VYQ24_001918"
     CDS             1635..>1720
                     /locus_tag="VYQ24_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1969014.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLS"
BASE COUNT          592 a          268 c          347 g          513 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg taatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca
//
LOCUS       DAVMEI010000213         1704 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.217, whole genome shotgun sequence.
ACCESSION   DAVMEI010000213 DAVMEI010000000
VERSION     DAVMEI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.217"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            4..1596
                     /locus_tag="VYQ24_001919"
     CDS             4..1596
                     /locus_tag="VYQ24_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1969015.1"
                     /translation="MKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMNLERFLKLS
                     IYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHSKSSGTYLS
                     WLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLMIMLLVPKI
                     FASKMREVSLNLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAGILLKMVIQ
                     RKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTGVIFTALGE
                     LGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNICYKYGDKEI
                     LKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIKKTSYLNMV
                     SNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNILDYYVGDD
                     GRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDREDLTVIIVT
                     HAPHPELKQYFTKIYQFPKDFI"
BASE COUNT          597 a          199 c          321 g          587 t
ORIGIN      
        1 aatataaaaa aatttagttt attaacgata tttattgttg ctggtcaatt attgctaatt
       61 tatgcagcaa ctattaatgc tctggtgttg aatgaattaa ttgcgatgaa tttagagcgg
      121 tttttgaaat tgtcaatcta ccaaatgatt gtctggtgtg ggataatatt ccttgactgg
      181 gtagtgaaaa attatcaggt tgaagtgatc caagagttta atctagagat tcgaaataga
      241 gttgccacag acatctctaa ctctacctat caagaatttc atagtaaatc atcaggaaca
      301 tatctttcgt ggctaaataa tgatgttcag actttaaatg atcaggcttt taaacaactt
      361 tttttagtaa taaaaggaat ttctggtact atatttgcag ttgtgactct taatcactat
      421 cattggtcat tgactgtagc caccttgttt tcattaatga ttatgctact tgtaccaaaa
      481 atctttgcat cgaaaatgcg agaagttagt ctaaatttaa ctagccaaaa tgaagctttt
      541 ttaaaatcta gtgagactat attgaatgga tttgatgtgt tagcgtcctt gaatctttta
      601 tatgtattgc ctaagaaaat taaagaagca ggaattttat taaagatggt tatacaaaga
      661 aagacaactg tagaaacgtt agcaggcgct attagcttct ttctcaatat tttttttcag
      721 atatctctcg tttttttaac aggctatctt gcaataaaag gaatagtgaa aattggtact
      781 attgaagcaa taggagcact aacaggtgtt atttttacag cgctaggtga attaggaggt
      841 caattatcct ctattattgg tacgaagcct atttttttaa aattgtattc aattaatcca
      901 attgagtcaa ataaaatgaa tgatatcgaa ccaaatgagg tgaatagaga ttttccgtta
      961 tatgaagcaa aaaatatttg ctataagtat ggagataaag aaatattaaa aaacttaaat
     1021 ttttgttttc aacgtaatga aaagtattta attttaggtg aaagtggaag cgggaaatct
     1081 acattattaa aattattgaa tggctttttg agagattata gtggagaatt gcgattctgc
     1141 ggggatgata taaaaaaaac ctcctattta aatatggttt cgaatgttct atatgtagat
     1201 caaaaagctt atttgtttga aggtacgatt agagataata ttttattgga agaaaattat
     1261 actgatgaag aaatactaca gtctttagag caagttggtt tgagtgtaaa agattttcct
     1321 aataacattt tagattatta tgttggtgat gatgggagat tactgtcagg agggcagaaa
     1381 caaaaaatta ctttagctag agggctaatt agaaataaga aaatagtatt aattgacgag
     1441 ggaacttctg ctatcgatag gagaacttcg ttagcgattg aacgtaagat attagataga
     1501 gaggatttga ctgtcattat tgttacccat gctccgcatc cggaacttaa acaatatttt
     1561 actaagatat atcaatttcc aaaggatttt atttaagaat ataggggagg gaaacatgag
     1621 attctattgt gaagcttatg aggtaattac tgaagagaaa aaagtatcat ttttttatgt
     1681 agatgaaaaa cccttaaggt atct
//
LOCUS       DAVMEI010000214         1699 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.218, whole genome shotgun sequence.
ACCESSION   DAVMEI010000214 DAVMEI010000000
VERSION     DAVMEI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.218"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(740..1588)
                     /locus_tag="VYQ24_001920"
     CDS             complement(740..1588)
                     /locus_tag="VYQ24_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1969016.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
BASE COUNT          507 a          252 c          366 g          574 t
ORIGIN      
        1 caaaaaaata tgctattatg ttaaaaaaat atttttcaaa atataaatgg acggatttat
       61 tttggatttt atttgttatt ttgacctgcc tctatattgg taaccatgat ttgtttactc
      121 tcaatcatca agaattctct tttcgtggta gcgtttgggg tctggtactg gccttatatc
      181 acttattgtt cattgataag tttgtaattt cgaatcgaaa ataaagatta gaactatgat
      241 tgactggcta cttttaatat tgattttgga ggaagatttt gtctctatta tttattattt
      301 taaatttatt tattttgtat aagatctatt ctttgaggcg ggaaaaatcg aaatacttga
      361 tttatacggc ctatatcata tttggggtaa atgtagtata tggtattcaa tggttattaa
      421 gagaactgat ttcaactatt tccccttaat gtgtatggaa actataacaa aacgcataag
      481 attaaggttt gtgtacctgt tcttatgcgt ttttataata ttgaagattt tcggtaagtt
      541 ttctactttt gcgaagtcta gtttatactc aatgaaaatc aaagagtaaa ctagccgtag
      601 gttgctcaaa gtacagcttt gaggttgcag ataaaactga cgaagtcagc tcaaaacatg
      661 gttttgaggt tgtagatgaa actgacgaag tcagtaacca tacgtacggc aaggcgacgt
      721 tgacgtggtt tgaagagtat tagaatgctt tcccaaccaa aatctctgct tcgatggtaa
      781 tctctgtcag ttggctccag tcaatcttgc tctggtcaac gtgaaagagg agaggagtca
      841 tactgagtag ggcttggagt tgttctgctg tgatagtctt agtcagagag gcagtttgac
      901 tagataggat ggtaaagtgt tcctggaaat gctccttgat atcttggtta gaatactcct
      961 tgtttgtcag ctggtcctgt actctttgac ggatttcttt gaggtgattt tcagttggga
     1021 taacctttat caagataccg tctttggata aaacgcgacg aaattctcca tagttggcag
     1081 gtgagaagat atcaagcaga atatccatgt tagcgtcttt tataggaagg cgtgccaagt
     1141 cgccaacgaa ccaattgact gcccagttgg gttcactttt agcagcgatt tggactgaat
     1201 ctttggagat atcaaaggca tagaaagttt tttcagagtg actttcttgt agtttgcgag
     1261 aatagaatcc ttcaccacaa ccgatatcca aaattgttgt ggtagttttt gagcttgcaa
     1321 gcaagtcaga tacagcatct aagatagctt ggtaaaagcc ggcttctagg atttgttgac
     1381 ggttttgaaa attttccttg tcatagttag cagattgctt gatttgaggt gctagattga
     1441 catagccaaa tttcgccaag tcaaaagaat gacggttgca gcacttgaaa ttagtctcta
     1501 acagagtcag attttcttga cagataggac aggcaaaggc agtcgcagaa gcaaaacgct
     1561 gaagtttggg cttgagattt gtattcatag tttaagtgta tcaaaagagg caaatgaaag
     1621 caactttagg aataagtaga tgggagattc agtagaaata aaactaattc tccaatttat
     1681 agaatgaaat tgcttttat
//
LOCUS       DAVMEI010000215         1682 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.219, whole genome shotgun sequence.
ACCESSION   DAVMEI010000215 DAVMEI010000000
VERSION     DAVMEI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.219"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            223..1584
                     /gene="dnaA"
                     /locus_tag="VYQ24_001921"
     CDS             223..1584
                     /gene="dnaA"
                     /locus_tag="VYQ24_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1969017.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT          573 a          290 c          296 g          523 t
ORIGIN      
        1 cctgaaataa ggctgttctt ttattttttt atctcacaag gttatccact atgtttttcg
       61 ataaaaagct taataaatca ataatttctt cttttatccc caacctgtgg ataaagtttg
      121 gtaacattgt ggattatttt tcacagcttg tggaaaattc ttgctatcta tggtaaaata
      181 tctctagtat taaactttta aatagtaaag gaggagaaag gattgaaaga aaaacaattt
      241 tggaatcgta tattagaatt tgcacaagaa agactgactc gatccatgta tgatttctat
      301 gctattcaag ctgaactcat caaggtagag gaaaatgttg ccactatatt tctacctcgc
      361 tctgaaatgg aaatggtctg ggaaaaacaa ctaaaagata ttattgtagt agctggtttt
      421 gaaatttatg acgctgaaat aactccccac tatattttca ccaaacctca agatacgact
      481 agctcacaag ttgaagaagc tacaaattta actctttatg actatagtcc aaagttagta
      541 tctattcctt attcagatac gggattaaaa gaaaagtata cctttgataa ctttattcaa
      601 ggggatggaa atgtttgggc tgtatcagcc gctttagctg tctctgaaga tttggctctg
      661 acctataacc ctctttttat ctatggagga ccaggccttg gtaagactca cttattaaac
      721 gctattggaa atgaaattct aaaaaatatt cctaatgcgc gtgttaaata tatccctgcc
      781 gaaagcttta ttaatgactt tcttgatcac ctaagacttg gggaaatgga aaagtttaaa
      841 aagacctatc gtagtcttga tcttttgtta atcgatgata tacagtcact cagcggaaaa
      901 aaagtcgcaa ctcaggaaga atttttcaat acctttaacg cccttcatga caagcaaaaa
      961 cagattgtcc taacgagtga tcgtagtcca aaacatctag aagggctcga ggagaggctt
     1021 gtcacgcgtt ttagttgggg attgacacaa actatcacac cccctgactt tgaaacacgt
     1081 attgccattt tacaaagtaa gacggaacat ttaggctaca atttccaaag tgatactcta
     1141 gaatacctag ctgggcaatt tgattcaaat gttcgagatc ttgagggagc catcaacgac
     1201 atcactttaa ttgccagagt aaaaaaaatc aaggatatca ctattgatat tgctgcagaa
     1261 gccattagag cccgcaaaca agatgttagc caaatgctcg tcatcccaat tgataaaatc
     1321 caaactgaag ttggtaactt ttatggtgtt agtatcaaag aaatgaaggg aagtagacgc
     1381 cttcaaaata ttgttttggc ccgtcaagta gccatgtatt tatctagaga actaacagat
     1441 aatagtcttc caaaaattgg gaaggaattt gggggaaaag atcataccac agtcattcat
     1501 gcccatgcca aaataaaatc tttgattgat caagacgata atttacgttt agaaattgaa
     1561 tcaatcaaaa agaaaatcaa ataatttgtg gataactttt agttttttat cttttttatc
     1621 cacatttttt aaacaagcta aaaaacttga tatgacttgt ttaaaggctg ttttccacag
     1681 at
//
LOCUS       DAVMEI010000216         1679 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.220, whole genome shotgun sequence.
ACCESSION   DAVMEI010000216 DAVMEI010000000
VERSION     DAVMEI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.220"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..155
                     /locus_tag="VYQ24_001922"
     CDS             <1..155
                     /locus_tag="VYQ24_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1969018.1"
                     /translation="INVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKGERN
                     ITNGFK"
     gene            142..348
                     /locus_tag="VYQ24_001923"
     CDS             142..348
                     /locus_tag="VYQ24_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969019.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISRVCYKGTQFSLIAVNSDMSLGR"
     gene            394..753
                     /locus_tag="VYQ24_001924"
     CDS             394..753
                     /locus_tag="VYQ24_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969020.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKETVLG"
     gene            881..1306
                     /locus_tag="VYQ24_001925"
     CDS             881..1306
                     /locus_tag="VYQ24_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969021.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
BASE COUNT          613 a          193 c          358 g          515 t
ORIGIN      
        1 tgataaatgt atgtgatgtt ggaaaaagaa taaaagaact tagaatatct tcaaatctta
       61 ctcaagataa gattgctgag tatttgtctt tgaatcaaag catgattgcc aaaatggaaa
      121 aaggtgaaag gaatatcacg aatggattta agtaataaag ctccaaatct tagaaaaaag
      181 ttgggagctg atggtgaatc gccgatagat atttttaaat tggtacaaaa gatagaaaat
      241 ttgacgctgg tattttatgg actcggaaag aatattagca gagtctgtta taaaggaact
      301 cagttcagtc tcattgcagt caattcagac atgtcattag gaaggtaaag attttcttta
      361 gcacatgaac tgtatcatct ttattatgat gaggtgaaga agagttcagt cagtcttatc
      421 ttgattggtg aaggagatga aactgaaaga aaagcggatc agtttgcttc ttatttttta
      481 attttcccat cttcactgta taggatggtt gaggaaatta gagaaaatgc caatagaact
      541 catcttgaag tagaagatat tataaaattg ggtcagtttt atggtatcag tcataaagct
      601 atgttatata gattgaggaa tgatggatac cttgatgcag aagaaattaa aaatatggat
      661 attagtgtta tagagacagc ttcaagatta ggctatgata caagtttata tcgtcctttg
      721 tcagaaagta aaaaggaaac ggtattagga taatatatta atttaactga acaactttta
      781 gaaaataaca gaatttcgca agggaagtat gaggaactgt tactagatgc tttcagatat
      841 gatattgtat atgggctaga tgaagagggg gagttgtcgt ttgactagtc gtgtatttat
      901 tgatgcagat tgtatttcag catttttatg ggttggcact gaacatcttt tagaaaagct
      961 ctattcgggt aaaattgtta ttccacaaga ggtgtatgat gaaatcaata tacctacaat
     1021 tccccattta aaatctagga tagaccagtt ggtaactaag ggttcagctg agattgtgag
     1081 catagacatt ggaactgaag aatacgcatt atatagagat ttaacaagaa attatgatag
     1141 taacaagatt attggtaagg gagaagcgac ttctattttc ttagcgaaaa agcataatgg
     1201 gatattagga agtaataacc taagagatgt taaaccatat gtagaagaat tttctttaga
     1261 acatatgaca acaggagata tactgattga agcgtttaaa gcgtaattta ttactgaaca
     1321 agagggcaat catatctgga ataatatgct taaaaagaga aggaaaattg gtgcaaattc
     1381 attttcagac tatcttcgtg gaagtattca ccaaaataga caaaaataaa tttggataaa
     1441 tcgaactcac tattcaggag gcatatgagc aattcgaaaa agaaaagtgt caaattgagc
     1501 ctataggagt agaagtgaaa tagtaagtcc tgcatagtgg atgagagaaa agttctcctt
     1561 gaagttttcc tgaactatca gtcgcatgtc aaacgatatg tagggtaatg tgagagggga
     1621 tagcgagtag tttttggtta ttttatcaaa aaacttatat tttattatac cgaatgata
//
LOCUS       DAVMEI010000217         1656 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.221, whole genome shotgun sequence.
ACCESSION   DAVMEI010000217 DAVMEI010000000
VERSION     DAVMEI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.221"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            101..628
                     /locus_tag="VYQ24_001926"
     CDS             101..628
                     /locus_tag="VYQ24_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1969022.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPIVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            640..1155
                     /locus_tag="VYQ24_001927"
     CDS             640..1155
                     /locus_tag="VYQ24_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1969023.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1152..1619)
                     /locus_tag="VYQ24_001928"
     CDS             complement(1152..1619)
                     /locus_tag="VYQ24_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1969024.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVIYYKATEFSGSLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT          463 a          357 c          313 g          523 t
ORIGIN      
        1 ttttaactaa aaaatgatct ataaacccaa gtaaattctt gctttattgt ctggtttatt
       61 gtaccatact agtgtatatg cagttaaaaa ggagattctt gtgaatacac ggaaaaagac
      121 acaatttatg acaatgacag cccttttaac ggctattgcg attttgattc caattgttat
      181 gcctttcaag attgtcattc cacctgcttc ctatactttg gggagccaca tcgctatttt
      241 tatagccatg ttcttgtcgc ccttgatggc agtttttgtc atcctagcct ctagttttgg
      301 atttttgatg gctggctatc ccattgttat cgtttttcgg gccttttccc atatatcttt
      361 tggtgcttta ggagctcttt acctacaaaa attccccgat accctagata aaccaaaatc
      421 ttcctgggtt ttcaattttg ttttggctgt tgttcatgcc cttgctgaag tattagcctg
      481 tgtccttttt tacgcaactt cgggtaccaa tgtagaaaac atgttctatg ttctatttgt
      541 actagttgga tttggcacaa ttatccatag tatggtagac tatacattag cactagctgt
      601 ctataaagtg cttcgaaaac gccgttaaaa ggaaaagcta tgacaaaaga tcgcaaacaa
      661 gccctgctcc aactcttgaa agaagctcct aaagccctta atggccaaag tttggcggaa
      721 cattttcatg tcacacgtca ggtcattgtg caggacattg caattttgag agccgatggc
      781 gctcctatcc tatccaccaa tcgtggctac atctataagc aaattgatac caatccttat
      841 gttcacaaac ttttcaaagt gaaacatgaa gttgaagaaa tcggtcagga actccttgct
      901 attgtcgata atggtgggca tgtccagaat accttgattg accatcccgt ttatggagaa
      961 attgaaacct tgcttaaact gtcttgtcgc agagatgtgc aacattttct agaacaagtc
     1021 gagcattctg actttagacc tctatctgaa ttgacagatg gcatccatta ccacctagtc
     1081 gaagccgaaa cacaacaaga cctccactat atcgaggagg ccttggatca gctaggttat
     1141 ttagtaaaag actagaagat tttcttttcc caatcgtctt ctgtacggcg aggatagaaa
     1201 aactctgact tgtcgggagc ttccatcatt tccatcaatg gtagcatatc ataggccaga
     1261 tccaagtttg gaatctggtc tttttgcacc caagaaactt ctccttcatc tgaagaccga
     1321 aggctaccag agaactcagt cgccttataa taaatgacaa tgtagcgccc acctgtatct
     1381 agtggccaat ttttaatgcc gacaagttga ggattttgga tagtcaaccc tgtttcttca
     1441 tagatttcac gaatgacaga ctctgcgaaa gactcgccat tttctacatg acctccagga
     1501 aaggcataac cagaccagcg attgttttca ggggcgcgat actgcatcac cacgcgctga
     1561 gtttcgaggt cttcaatcag acagatattt gttaaaattg ttaattggga acgggacata
     1621 aatttactcg ctttctaatt tattaaattt tcagac
//
LOCUS       DAVMEI010000218         1640 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.222, whole genome shotgun sequence.
ACCESSION   DAVMEI010000218 DAVMEI010000000
VERSION     DAVMEI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.222"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            100..1575
                     /locus_tag="VYQ24_001929"
     CDS             100..1575
                     /locus_tag="VYQ24_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1969025.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVLYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          460 a          329 c          330 g          521 t
ORIGIN      
        1 tccagtattt gacaatattg aagcagctat taaagaacat ggattaagct aattctaatt
       61 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      121 atcggaaagg ttttcaatcc catcttagaa atgggtggac ctgtcatcat gttaatcatt
      181 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
      241 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
      301 tcagcatcac ttgcaaaatt cgttgaaaac actggtatac aattgagtat taccgacgtt
      361 ggttgggcac cacttgcaac aatcacttgg ggttctgctt ggacactata cttcttgctc
      421 atcatgttga ttgtcaacgt agtgatgctt gctatgaaga aaaccgatac acttgatgtc
      481 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
      541 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
      601 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
      661 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
      721 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
      781 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
      841 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
      901 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
      961 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1021 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1081 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1141 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1201 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     1261 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     1321 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     1381 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     1441 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtatta
     1501 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     1561 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     1621 taggaagcta gccgcaggtt
//
LOCUS       DAVMEI010000219         1521 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.223, whole genome shotgun sequence.
ACCESSION   DAVMEI010000219 DAVMEI010000000
VERSION     DAVMEI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.223"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            161..1339
                     /locus_tag="VYQ24_001930"
     CDS             161..1339
                     /locus_tag="VYQ24_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1969026.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQIILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          568 a          267 c          220 g          466 t
ORIGIN      
        1 tgtcaaggat ttgtgtaaac atttgattat tttttattat caatatataa taaaaagaga
       61 ccagtaagta atgaaaagct taaaaagatt aacatctaca tttatccata gcaaaaaatc
      121 ataggatttt aagaatctga caactaggaa gaaaactaat atgaccttat ctaagaaaca
      181 attacaacta agagctaaaa tactggaaac agtttacaca ttaggtccca tttcacgaat
      241 tgaaatcgct actaaaacag gaataacacc tgcaacaact agcagtatca caaatgatct
      301 gattaaagaa aatattctct tagaacttgg tgaagacgaa cacgatacaa gcgtagggag
      361 aaaaaaaatc ctgctagaca ttcaagctaa acgattttac tacattggtt gtgaactatc
      421 tgaaaaacat ttcacttttg ctttaggaga taatctagga aatatcttaa aggaagagaa
      481 agaaattgtt actaaacagt taatccaaga aaagggaaat caactcatta accagacact
      541 aaagcaattc ttaaataact gctctgatta tgaaatagaa gcaattggaa tcgcgctacc
      601 tggtcgctat ttagataatt ataaaataac aacaaacaat cctttatggc aacatatcga
      661 cttagaaatg attcaatccc actttgataa gcctttgttt ttctctaaca atgttaactg
      721 tatggctata ggtaaacgcc tatttagtcg ccaacagaat gaccctaatt ttgcttattt
      781 tcattttgct agaggtatgc actgttctta tatctacgac ggaaacattt atgggaaagg
      841 aaatctaatg attggagaaa tagggcatac agttgtttca tctgagggag aagaatgtag
      901 ctgcgggcgc aagggatgct tacaaacttt tgctggagaa tcttggttaa tcaaaaaatc
      961 aaaaattcta tatcatcaat ctccctattc tctacttcct agtttagtca aaaatgccga
     1021 tgatattgat atccaaatta ttttaaccgc ttatcaatta ggtgacactg gaataatcac
     1081 acttatccat caagccttac tctatcttag tcaaaccatt ttaaatatca gtatgatgat
     1141 tgactctcaa aaaatctacc tacatagtcc tttactaaca aatcaacaca tcatccaaaa
     1201 actatatagt gaaatgaact acaaacccaa actgctctat aatcgcctac ctgaagtcat
     1261 tattgaacct tacaatgatt ttacagccgc acactcagct attgcacttt gtttgtatca
     1321 tacaattcta cattcttaat tttctattac atttaataaa taggctaaaa agagggagta
     1381 tatcataaaa agcgaataaa cctgttttct gaaaatcaga attctgtttt gttcgctttc
     1441 tttatatttg gaattatact cctatctccc ctttttcata atgtataata aaatgaaata
     1501 ataacaggac gaatcgatcg g
//
LOCUS       DAVMEI010000220         1515 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.224, whole genome shotgun sequence.
ACCESSION   DAVMEI010000220 DAVMEI010000000
VERSION     DAVMEI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.224"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(68..470)
                     /locus_tag="VYQ24_001931"
                     /pseudo
     CDS             complement(68..470)
                     /locus_tag="VYQ24_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(546..1322)
                     /locus_tag="VYQ24_001932"
                     /pseudo
     CDS             complement(546..1322)
                     /locus_tag="VYQ24_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1246..1440)
                     /locus_tag="VYQ24_001933"
     CDS             complement(1246..1440)
                     /locus_tag="VYQ24_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1969027.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
BASE COUNT          406 a          336 c          292 g          481 t
ORIGIN      
        1 agaaaataac caccctcgct tttttaaact gagctcaggc taaaagagtc cactggactc
       61 ttttactccg tcccataacc aatgatttaa tggcgcgttc agctagatta ttggaaagga
      121 ccagatgtcc gtcttttaaa atggtcttaa aggtttcttt atacttgagg ctgtattcaa
      181 ttgcccttcc tagttttgaa cctgctaaaa ctgactgacg gcggcaccaa gcaaagaagt
      241 cttccattag gggctggacg tttctgtagt cgttcatcag ctggcaaagc ctcccagtct
      301 ctttccaagg caaataactg gtcacgatag gctaaacctt tagctcctaa ggatgattta
      361 tctgcttgct tgggggtcgc ttcaaaaaat ttccttctca catgcgccca acatccaaca
      421 agttcagctt cttccagttg acgataagct tctagcggtt cgcagtttac caagcttagc
      481 gtcgccttgc tcctaaacct tagactgcta tcgcataggc agaactagag gactaaagtt
      541 ctaagttact gccgcaacat atcacaatgc atatagccag aataatttcc taggaattct
      601 tgcactaccg aaccactccg acgctgatca tggtggtaca gcgtaatccc ttgtttctca
      661 gctttcccag acaaaaaagt ccaatagtaa gtcagctgac tatcactttc taagacccga
      721 taagaggttt catctgcatg aagaagagct tgttctaaca acttttctcg taaaaggtta
      781 taaagggact ctaaatagta ttgactcgcc ttgatatgcc aattagcaat ttccttacgt
      841 gtgattggta aacccatctt agcccaatct tcttcttagc gataattggg taccttcaga
      901 ataaacttct gatggatggt gtgagcgata atagaagctg agccaaggct atgcgccaaa
      961 ggggctttag gaataggagc tttcacgatt ttatcacttg gattattctt actgcatgtt
     1021 tggcatttgt aagcgtgttg gatatgattc actcttttta attgcgcaag aataaagacc
     1081 aattcttgtc gttgaaggct cgctccaatc tcttttagct ctccctgaca gtcagggcaa
     1141 atgcagttct ctaagcgatg atgaacttct tctgaatcaa attgggcaag aatagcttga
     1201 cgtttccctt tagctttctt acgtttatag gtgatttctt ctctttcaac tgggtaagtc
     1261 agagtcttct tccatatttt gttcctcttc aaaaagactg agttgtccag atgggcaaac
     1321 acttttctca gaggattttc catagagctt ttgcgttaga taagccactt gttcacgaag
     1381 aagggcaagt tcattggtga gactatcaat tgtagcactc tgttgttgaa tgattttcaa
     1441 tcttctccca tagactttcc tccttatttc ctaactttat tatatagtgt attgaatcta
     1501 taacagtaca ccttg
//
LOCUS       DAVMEI010000221         1508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.225, whole genome shotgun sequence.
ACCESSION   DAVMEI010000221 DAVMEI010000000
VERSION     DAVMEI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.225"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            521..892
                     /locus_tag="VYQ24_001934"
     CDS             521..892
                     /locus_tag="VYQ24_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969028.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
BASE COUNT          472 a          197 c          268 g          571 t
ORIGIN      
        1 ttagaaaaat attttcaaaa tcttccaaaa aggatacgga tagtactctc taaaaaataa
       61 atttctcccc tgtatttgct agcttaattt acaggggagt ttctttaata ttgtttcgcc
      121 tgtcaaaatg atatgtgata gcaggatgaa aagtctacaa acaagttggt atcaatacaa
      181 tataaagagg aaagtatgtt tttcatgctt gcttttttaa tttttacaat tcaagaggtt
      241 ttgatgacca tttatgattt gagtgatcca aggtcaaaat aagtgctata ctagcagtgt
      301 aaatgttctt gctcaactaa gataaatcgc acatttagga ggaaaactta tgaagaaaaa
      361 aatactgatt attttcgttc tgtatctgat catgtccatc tttctttatc cgcttaggga
      421 gagtatttgg tataatctat tttataccat agcctatatg attgcggtta tgatctattt
      481 ttctttaatt aaaaagaaag aaaagaaatg aaagattttc ttgattaaaa agaatcatat
      541 tctttgtgag attgttcctg ggtcagttgc ctttgcttgt ttctacttat ctatttctat
      601 ttcgtcagtt ttccaatttc ttttagtagt tattaacttg acttctattt tggttactgt
      661 ttatcttact agggaaatga gattaagaga gtttgaagat gatgatttgg ttagtcctag
      721 aaccaatcaa ctcatgttta tcggcttgac aggctttatg tctattattt gtttgtatag
      781 aggtatcaca gcaggagaat cttatcaaca actaatcgct tatattggtg ctattctttg
      841 cttgatcatc atgcttctac tcatttgggg tttgaagtat tataaaaagt aggggctaat
      901 gaagtttttg caatttagga ggtttcgatg acaattcaag atttggatga aaaattttaa
      961 aaaacaatga tatactaaac ttgtcaaagt tgcaacgggt gctgctacat tatgttaaaa
     1021 atcgctcttg attgataagt tggatttgtt aggaaaggag taaataatta tggataagaa
     1081 aaagatagtt tcgactataa tatgtatagt atttcttgta gtgtcagtag ataatttttt
     1141 tagagactta accccgttac tgttcatttt aaatattatt ggcctgtctt gcttttcagt
     1201 gctaacatac attaacataa aagagatact cttaaatata agtaaatgat gatttggtta
     1261 gtcctagaac caatcaactt atgtttatcg gcttggcaga ttttatgttt gtcatttgtt
     1321 tgtatagagg tatttcagaa acagaattct atcaacaact aatcgcttat ataggtgtat
     1381 tctctgcttg tttatcacgc ttctgctcat gtggggcttg aagtattata aaaagtagct
     1441 ttctatcctt ttctgacagg atatttgagt agaaggctct attaaattgt tagtgaaata
     1501 aatgaatg
//
LOCUS       DAVMEI010000222         1507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.226, whole genome shotgun sequence.
ACCESSION   DAVMEI010000222 DAVMEI010000000
VERSION     DAVMEI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.226"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            74..>1507
                     /locus_tag="VYQ24_001935"
     CDS             74..>1507
                     /locus_tag="VYQ24_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1969029.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVAKQSQAER
                     DYDAAMKKSEAAKKEYEEAKKDLEEAKAAQKKYEDDQKKTEEKAKLVQKADEERQKAN
                     VAVQKAYLKLREAQEQLNKSPNNKKNAAQQKLKDALAHIDEVTLKQKEAEANFNKEQA
                     KVVPEADKLAETKKKAEQAEKKEPELAKKVAEAKAKAEEAEKKAVEAKQKVDAEKYAL
                     EAKIAELEYEVQGLEKELKEIDESDSEDYIKEGLRAPLQSKLDAKKAKLSKLEELSDK
                     IDELDAEIAKLEKDVEDFKNSDGEQAEQYLVAAKKDLDAKKAELENTEADLKKAVDEP
                     ETPAPAPAPKPAPAPAPTPEAPAPAPKPAPAPKPAPAPKPAPAPKPAPAPKPAPAPKP
                     APAPKPAPAPKPAPAPKPETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNAN
                     GAMATGWAKVNGLWYYLNSNGAMATGWL"
BASE COUNT          583 a          297 c          324 g          303 t
ORIGIN      
        1 aaatatttac ggggggagta tacttaatat aagtatagtc taaaaatgac tatcagaaaa
       61 gaggtaaatt tagatgaata agaaaaaaat gattttaaca agtctagcca gcgtcgctat
      121 cttaggggct ggttttgttg cgtctcagcc tactgttgta agagcagaag aagctcccgt
      181 agctaagcag tctcaagctg agagagacta tgatgcagca atgaaaaaat ctgaagctgc
      241 taagaaagaa tacgaagaag ctaagaaaga tttagaagaa gcaaaagctg ctcagaaaaa
      301 atatgaagac gatcaaaaga aaactgaaga gaaagcaaag cttgttcaaa aagcggatga
      361 agagcgtcaa aaagcgaatg tggcagttca aaaggcgtat ttaaaactcc gagaagcaca
      421 agaacaattg aacaaaagtc caaacaataa gaaaaatgcc gctcaacaga aattaaaaga
      481 tgcattagct catattgatg aggtaacatt aaaacaaaaa gaggcggaag cgaatttcaa
      541 taaggaacaa gcaaaagtag ttcctgaagc agataagtta gctgagacta agaaaaaagc
      601 agaacaggct gaaaaaaaag aaccagaact tgctaaaaaa gtagcagaag ctaaagcaaa
      661 agcagaagaa gctgagaaaa aagctgttga agccaaacaa aaagtggatg ctgaaaaata
      721 tgctcttgaa gctaaaatcg ctgagttgga atatgaagtt cagggactag aaaaagagct
      781 caaagagatt gatgagtctg actcagaaga ttatattaaa gaaggtctcc gtgctcctct
      841 tcaatctaaa ttggatgcca aaaaagctaa actatcaaaa cttgaagagt tgagtgataa
      901 gattgatgag ttagacgctg aaattgcaaa acttgaaaaa gatgtagaag atttcaaaaa
      961 ctcagacggt gagcaagctg aacaatatct tgttgcagct aaaaaagact tagatgctaa
     1021 aaaagctgaa ttagaaaata ctgaagctga ccttaagaaa gcagttgatg agccagaaac
     1081 tccagctccg gctccggctc ctaaaccagc cccagctcca gctccaactc cagaagcccc
     1141 agctccagct cctaaaccag ctccggctcc taaaccagcc ccagctccta aaccagctcc
     1201 ggctcctaaa ccagctccgg ctcctaaacc agctccggct cctaaaccag ctccggctcc
     1261 taaaccagct ccggctccta aaccagctcc agctcctaaa ccagaaactc caaaaacagg
     1321 ctggaaacaa gaaaatggta tgtggtactt ctacaatacg gatggttcaa tggcaacagg
     1381 ctggctacaa aataatggct catggtacta cctcaacgct aatggtgcta tggcaacagg
     1441 ttgggctaaa gtcaacggct tatggtatta cctcaacagc aatggtgcta tggcgacagg
     1501 ttggcta
//
LOCUS       DAVMEI010000223         1477 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.227, whole genome shotgun sequence.
ACCESSION   DAVMEI010000223 DAVMEI010000000
VERSION     DAVMEI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.227"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            42..620
                     /locus_tag="VYQ24_001936"
     CDS             42..620
                     /locus_tag="VYQ24_001936"
                     /inference="COORDINATES: protein motif:HMM:NF024584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEV1969030.1"
                     /translation="MIRELAKLAEQTGCIERIIIQKIQLNNNDEDTVTVKNIASESPK
                     TQFVHKKFIRFPKVRNYSKYGTLEKLEKGKSVFLYHLIGAKTPFKCLKPWIKNELAVN
                     SDGNYVPCCLTLNSSINLGNIENETISEVFDSEEHYLLRESVFLNSLKNNSTCLTCPI
                     NEPNLEIPNELAESQSIEKLKKYIISDWRFFP"
     gene            617..>1477
                     /locus_tag="VYQ24_001937"
     CDS             617..>1477
                     /locus_tag="VYQ24_001937"
                     /inference="COORDINATES: protein motif:HMM:NF015983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1969031.1"
                     /translation="MKKIKYDNPIGAQIELTGRCNLKCHHCYNSSGDFGRFDIKDDAW
                     LSFFEKLNESYSFPTVNITGGEPMLRKDLLLKVLNIFSNKNPYTRIRIMTNGYYLDKT
                     FLEYLSLINNPIEFQISLDGADKAEHQAVRIVRNSWEKAINSCIMVVNSGYKLQIAST
                     ITTSNYDKIERLFKLAVLVGATSLGIGSALPLGRGIRDVKGLILPSQLREEVSMKLKA
                     LKQDYESFISVNLTSSLSEVYSKYASLEQDWLIIDHRGNVKIDTRLPYIVGNISSKSI
                     EELWLLVQEKY"
BASE COUNT          527 a          196 c          282 g          472 t
ORIGIN      
        1 gaggaaaaat ttctaaggaa acaattgata gaagtatagt gatgatacgg gaacttgcta
       61 agttagctga acaaacaggg tgtatcgaga ggataataat tcaaaagatt caattaaaca
      121 ataacgacga ggatacagta acggtaaaaa atatagcatc tgagagtcca aaaactcagt
      181 ttgtacataa aaaatttata aggtttccga aggtaagaaa ttattctaaa tatggtactt
      241 tagaaaaatt agaaaaaggt aagagtgttt ttctttacca tttaattgga gctaagaccc
      301 cttttaaatg tttaaagcct tggattaaaa atgaactagc cgtaaattcc gatgggaatt
      361 atgttccctg ttgcctgact ctgaattctt cgattaattt gggaaatatt gagaacgaaa
      421 caatttcgga ggtatttgac agtgaggaac actatttact acgggagtca gtatttctaa
      481 attctcttaa aaataattca acctgtctaa cttgtccgat aaatgagcca aacttagaaa
      541 ttccaaatga gttggctgag tcccagtcaa ttgagaaatt aaaaaagtat attatttccg
      601 attggaggtt tttcccgtga aaaaaataaa atatgataat ccgataggtg cccagataga
      661 attaactgga cgttgtaatt taaaatgcca tcattgttat aattcttcag gagattttgg
      721 gagatttgat attaaagacg atgcatggtt atcatttttt gaaaaactca acgaaagtta
      781 ctcttttcca acagttaata ttactggtgg agagcctatg ctgagaaaag atttattgtt
      841 aaaagtctta aatatttttt caaataaaaa tccttataca cgcattagaa ttatgacgaa
      901 tggctactat ttggataaga cttttttgga atacctttca cttattaata atccaattga
      961 atttcaaata tctttagacg gggcagataa agcagaacat caggcagtta ggatagtgag
     1021 aaattcttgg gaaaaagcaa taaattcctg tattatggta gtaaattcag gatataagtt
     1081 gcaaatagcg agcactatta caacatccaa ttatgataag atagaaagac tgtttaaact
     1141 tgcagtactt gtgggagcga cttcattagg tattggttca gctttgcctt taggacgagg
     1201 gattcgtgat gtgaagggct taattttgcc gtcgcaactt cgtgaagaag tttctatgaa
     1261 gttgaaagct ctaaagcaag attatgagtc gtttatttct gttaatttaa ctagctctct
     1321 atctgaagta tatagcaaat atgcatcttt agaacaggat tggctaatta tagatcacag
     1381 aggtaatgta aaaattgata ctaggttgcc ttatattgtt ggaaatattt catctaaatc
     1441 aattgaagaa ctttggttat tagttcaaga aaagtat
//
LOCUS       DAVMEI010000224         1470 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.228, whole genome shotgun sequence.
ACCESSION   DAVMEI010000224 DAVMEI010000000
VERSION     DAVMEI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.228"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            172..>1470
                     /locus_tag="VYQ24_001938"
     rRNA            172..>1470
                     /locus_tag="VYQ24_001938"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          453 a          278 c          421 g          318 t
ORIGIN      
        1 cctgctttgc acgcaggagg tcagcggttc gatcccgcta ggctccattg gtgagagatc
       61 accaagtaat gcacattgaa aattgaatat ctatatcaaa tagtaacaag aaaataaacc
      121 gaaaacgctg tagtattaat aagagtttat gactgaaagg tcagaaaata aggttaagtt
      181 aataagggcg cacggtggat gccttggcac taggagccga cgaaggacgt gacaaacgac
      241 gatatgcctt gggtagctgt aagtaagcga tgatccaggg atttccgaat gggggaaccc
      301 aacaggtaat acctgttacc tacatctgtt aaggatgtga ggaggaagac gcagtgaact
      361 gaaacatcta agtagctgca ggaagagaaa gcaaaagcga ttgccttagt agcggcgagc
      421 gaaacggcag aagggcaaac cgaagagttt actcttcggg gttgtaggac tgcaatgtgg
      481 actcaaagat tatagaagaa tgatttggga agatcagcca aagagagtaa tagcctcgta
      541 tttaaaatag tctttgtact tagcagtatc ctgagtacgg cgggacacgt gaaatcccgt
      601 cggaatctgg gaggaccatc tcccaaccct aaatactccc tagtgaccga tagtgaacca
      661 gtaccgtgag ggaaaggtga aaagcacccc gggaggggag tgaaatagaa cctgaaaccg
      721 tgtgcctaca acaagttcga gcccgttaat gggtgagagc gtgccttttg tagaatgaac
      781 cggcgagtta cgttatgatg cgaggttaag ttgaagagac ggagccgtag ggaaaccgag
      841 tctgaatagg gcgccttagt atcatgacgt agacccgaaa ccatgtgacc tacccatgag
      901 caggttgaag gtgcggtaag acgcactgga ggaccgaacc agggcacgtt gaaaagtgct
      961 tggatgactt gtgggtagcg gagaaattcc aaacgaactt ggagatagct ggttctctcc
     1021 gaaatagctt tagggctagc gtcgacatta gagattcttg gaggtagagc actgtttggg
     1081 tgaggggtcc atcccggatt accaatctca gataaactcc gaatgccaat gaattatggt
     1141 cggcagtcag actgcgagtg ctaagatccg tagtcgaaag ggaaacagcc cagaccacca
     1201 gctaaggtcc caaaataatt gttaagtgga aaaggatgtg gggttgcaca gacaactagg
     1261 atgttagctt agaagcagct attcattcaa agagtgcgta atagctcact agtcgagtga
     1321 ccctgcgccg aaaatgtacc ggggctaaaa caatttaccg aagctgtgga tacctttata
     1381 ggtatggtag gagagcgttc tatgtgtgat gaaggtatac cgtgaggagt gctggaacgc
     1441 atagaagtga gaatgccggt atgagtagcg
//
LOCUS       DAVMEI010000225         1466 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.229, whole genome shotgun sequence.
ACCESSION   DAVMEI010000225 DAVMEI010000000
VERSION     DAVMEI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.229"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            51..968
                     /gene="adcAII"
                     /locus_tag="VYQ24_001939"
     CDS             51..968
                     /gene="adcAII"
                     /locus_tag="VYQ24_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1969032.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            976..>1466
                     /locus_tag="VYQ24_001940"
     CDS             976..>1466
                     /locus_tag="VYQ24_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1969033.1"
                     /translation="MKINKKYLAGSVAVLSLSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHN"
BASE COUNT          503 a          245 c          322 g          396 t
ORIGIN      
        1 tttcaggagt agaatattta ctagttaatt aaatggttaa ggagttgttc atgaagaaac
       61 aaaatttatt tttagtcctg ttaagtgtct ttcttttgtg cttgggggct tgtggtcaaa
      121 aggaaagtca gacaggaaag gggatgaaaa ttgtgaccag tttttatcct atctacgcta
      181 tggttaagga agtatctggt gacttgaatg atgttcggat gattcagtca agtagtggta
      241 ttcactcctt tgaaccttcg gcaaatgata tcgcagccat ctatgatgca gatgtctttg
      301 tttaccattc tcatacactc gaatcttggg caggaagtct ggatccaaat ctaaaaaaat
      361 ccaaagtgaa ggtcttagag gcttctgagg gaatgacctt ggaacgtgtc cctggactag
      421 aggatgtgga agcaggggat ggagttgatg aaaaaacgct ctatgaccct cacacatggc
      481 tagatcctga aaaagctggc gaagaagctc agattatcgc tgataaactt tcagaggtgg
      541 atagtgagca taaagagact tatcaaaaaa atgcgcaagc ctttatcaaa aaagctcagg
      601 aattgactaa gaaattccaa ccaaaatttg aaaaagcgac tcagaaaaca tttgtaacac
      661 aacatacagc cttttcttat ctagcgaaga gatttgggct taatcaactt ggtattgcag
      721 gtatctctcc tgaacaagaa ccaagtccac gacaactaac agaaattcag gaatttgtta
      781 agacctataa ggttaaaacg atttttacag aaagtaacgc ttcttcaaaa gtagctgaaa
      841 ctcttgtcaa atcaacaggt gtgggtctta aaactctgaa tcctttagag tcagacccac
      901 aaaatgacaa gacctattta gaaaatcttg aagaaaatat gagtattcta gcagaagaat
      961 taaagtgagg aaagaatgaa aatcaataaa aaatatctag caggttcagt ggcagtcctt
     1021 tccctaagtg tttgttccta tgaacttggt cgtcaccaag ctggtcaggt taagaaagag
     1081 tctaatcgag tttcttatat agatggtgat caggctggtc aaaaggcaga aaatttgaca
     1141 ccagatgaag tcagtaagag agaggggatc aacgccgaac aaattgttat caagattacg
     1201 gatcaaggtt atgtgacctc tcatggagac cattatcatt actataatgg caaggttcct
     1261 tatgatgcca tcatcagtga agaacttctc atgaaagatc cgaattatca gttgaaggat
     1321 tcagacattg tcaatgaaat caagggtggc tatgtgatta aggtagacgg aaaatactat
     1381 gtttacctta aagatgcggc ccatgcggac aatattcgga caaaagaaga gattaaacgt
     1441 cagaagcagg aacgcagtca taatca
//
LOCUS       DAVMEI010000226         1461 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.230, whole genome shotgun sequence.
ACCESSION   DAVMEI010000226 DAVMEI010000000
VERSION     DAVMEI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.230"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            277..1158
                     /locus_tag="VYQ24_001941"
     CDS             277..1158
                     /locus_tag="VYQ24_001941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1969034.1"
                     /translation="METVVLQEILNIQNPEDFYVKLNKIDQFGIDTKSLYINDQPKLL
                     EQMGYLYDTLRKTNKPHFNYVMDRPYTIHLIPYDEANKLWLFVGAYSQSGTYQQTYED
                     RVTTYYKLNLAPEHSKLKGRLIVKFERPDGSQHVRIGLESATAQGFKLHSILEREISS
                     VEFQDYRNVRLTYQELKSIIKNQNPTWKTALSHLNAIYLQTDTKTGKQYVGSAYGKQK
                     LWGRWTEYVETYHGVNKALKELFKKEGASYFEDYFTYMLLEVLPSDNKEIGNTVISRE
                     SWWKIALQTREFGYNCN"
BASE COUNT          530 a          220 c          270 g          441 t
ORIGIN      
        1 ttataaaatg tgaagataat catagcttac cctgttttta tgatcagatt ggttttttag
       61 aaatataaaa acaatttttt cattgcaaag tgaaagcgag gtattgagat gagctaaaat
      121 atagaaatca aaatccaaca agcctttcgt aatttcgtaa tattgtggaa agttttgatt
      181 aacgatatgt aactggggga ttgttgaaaa aagatgctat tttaatcgca agatttagaa
      241 agtaagtagt tcactacaaa ttgaaaggaa tttaaaatgg aaacagtagt attgcaagaa
      301 atcttaaaca ttcaaaaccc agaagatttt tacgtaaaat tgaataaaat cgatcaattt
      361 ggaattgata cgaaaagcct ttatattaat gaccaaccaa aattattgga acaaatgggg
      421 tatctctatg acactttgcg taagactaat aaacctcatt ttaactatgt aatggaccgc
      481 ccttatacca ttcacctaat cccatatgac gaagctaata aattgtggct ctttgtaggg
      541 gcttactctc aaagcggtac ttaccaacaa acgtatgaag accgtgtaac gacttattac
      601 aagttgaatt tagctcctga gcacagtaag ttgaaaggcc gtttaattgt taaatttgag
      661 cgtcctgacg gttcacaaca tgtacgaatt ggtttggaat ctgctacagc tcaaggattt
      721 aagctgcact ctattttaga gcgtgaaatc tcaagtgttg aatttcaaga ttatcgaaac
      781 gttcgcctaa cttatcaaga gttgaaaagc attattaaaa atcaaaaccc tacatggaaa
      841 acagctttgt ctcatttgaa tgctatttac cttcaaacag atacaaagac aggaaagcaa
      901 tatgtaggtt ctgcttacgg gaaacagaag ctttgggggc gttggacaga gtatgttgaa
      961 acctaccatg gtgtaaataa ggccttgaaa gaactcttta aaaaagaggg tgcaagttat
     1021 tttgaagatt acttcactta tatgcttcta gaagttcttc caagcgacaa taaagaaatt
     1081 ggaaatacag taatcagtcg cgaatcttgg tggaaaattg ctcttcaaac tcgtgaattt
     1141 ggatataact gtaattaaat gcgaatactt gctcaacaat tatgtaatat actaatcgat
     1201 aaaaaaccaa caaaataaag agcctgaaac aaaatagttc tcaatcacat aaaagctaga
     1261 gttccgccaa ctggaaagct ctagcttttt tataactgag gaccattttg tcttagacac
     1321 tttgtaagta taatgagaat attgtttatt gaggtgtttt aaccatctag tctagagtta
     1381 gactaacgct agatagtgta gaaaggaaga gggagttaaa ctattgtttt acgaatatat
     1441 aatgtgtatg aaaggataca g
//
LOCUS       DAVMEI010000227         1460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.231, whole genome shotgun sequence.
ACCESSION   DAVMEI010000227 DAVMEI010000000
VERSION     DAVMEI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.231"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(302..1411)
                     /locus_tag="VYQ24_001942"
     CDS             complement(302..1411)
                     /locus_tag="VYQ24_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1969035.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT          548 a          263 c          218 g          431 t
ORIGIN      
        1 aatgaaataa gaataggacg aatagatcag gacagtcaaa tcgatttcta acaatgtttt
       61 agaattagag gtgtactatt ctagtttcaa tctactataa ataggtttca ttcgaacttt
      121 aatagactat gaaaaccagt ggaacttacc ctgacacgga attccactgg ttttttcgat
      181 ttttatcaga ttaacttcta ctattcttag ctgtgaaaat tagtttaaga ctttaactta
      241 ttttcagtct tctaatctaa aggccacttc cagtaaagaa aaatcagggt tctcctgact
      301 tttacttagc taattctctt tctcgttctt tcattatttt atgattttca tacaaacggg
      361 ataagaactt agaaatttct ttcaagatag aataggttgg cactgcgaca atcattccaa
      421 ctacaccata gatattgctt gacaacaaaa gtaaaactaa aatcgtgatt ggatgaacct
      481 tcataacact tcctacgatt cgaggatata agatattgcc atctacctgc tgaacaacaa
      541 gcatataaat cactgcaatc agcagtctat ggggatcagt gaatatattt gcgatgatca
      601 taggaatcaa accaatactt ggccccacat aaggaattaa attggctaca ccagaaaaaa
      661 tggcaaaaac taaagcatat tttaaaccaa taatactata gccaatataa gctaaacaac
      721 ctataatgat tgcgtcaatc gaaactccac taatatagcg agcaatcgtc gcatttaaat
      781 tctttaataa gcctgcaata tgcaagcgat ccctctttag aatcgttctt tcaagcatgg
      841 gcaagaattt atgtccatct aataagaaat aaaccaaaaa aactggagtc ataatcaaaa
      901 tcaaaacagt actgataaga gctgacaaga cgctccccac actatttgat acgctattta
      961 ggatattttg aagaatatca acataggata agtttaactg ctgaattgta gcttctacat
     1021 ccaaattctg gagcgcagga taattagata agtctatgat taagtcttgt actcgactat
     1081 aaatagtttg actagatata atcaaactag ataactgatt aatcaaaata ggtaagagat
     1141 agacaacacc tatgaccatt ccccagacca aagtacacaa ggtaattaaa ataccaagca
     1201 aacgattgag tttacagact ttatttaaga aagtaacaat agggtttgtc aaataataaa
     1261 gaaagccccc taataaaaat ggaatcataa ttgtattaag cacgctaaca aaagggttaa
     1321 tcaaagaccc catctgtctc cataggtaaa agatgatggt taagagtaaa atttctgtgg
     1381 tccaaaaaaa taatttattt ctacgaaaca tgagttacct ccctcacttt attttaccat
     1441 attttcaaaa agcttaacag
//
LOCUS       DAVMEI010000228         1435 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.232, whole genome shotgun sequence.
ACCESSION   DAVMEI010000228 DAVMEI010000000
VERSION     DAVMEI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.232"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..1373
                     /gene="gdhA"
                     /locus_tag="VYQ24_001943"
     CDS             27..1373
                     /gene="gdhA"
                     /locus_tag="VYQ24_001943"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1969036.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          427 a          286 c          313 g          409 t
ORIGIN      
        1 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
       61 ttgaaactgt gaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      121 ttttcaacac tttagaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      181 tggcacgtat tactgagcct gagcgcgtgg tttctttccg tgttccttgg gttgaccgtg
      241 atggaaaaat tcaagtaaac cgtggttacc gtgttcaatt caactcagct gttggaccat
      301 acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat
      361 ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt aaaggtggat
      421 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      481 tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg
      541 ttggtggacg tgaaattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg
      601 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      661 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
      721 ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatatgct cttcaaaaag
      781 caactgaact cggtgcaact gttatttctg tttctgactc aaatggttat gtcatcgatg
      841 aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga
      901 ctgagtatgc agctgaaaaa gcaactgcaa cctaccacga aggtactgta tggacttacg
      961 ctggaaacta tgacattgct cttccatgtg ccactcaaaa cgaaatcaac ggtgaagcag
     1021 ctaaacgttt ggttgctcaa ggcgttatct gtgtatctga aggtgccaac atgccgagcg
     1081 accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct gcaaaagctg
     1141 ccaacgctgg tggtgtagcc gtttcagctc ttgaaatgag ccaaaacagt cttcgcctct
     1201 catggactcg tgaagaagtt gatggccgtc tcaaagacat catgacaaac atcttcaaca
     1261 cagctaaaac gacttcagaa acatacggtc ttgataaaga ctaccttgca ggagctaaca
     1321 ttgctgcctt tgaaaatgta gcaaatgcca tgattgcaca aggtattgtt taagatttat
     1381 ttactcaatc ctcaatcact acgattgggg atttttagct tgaattaaaa aattg
//
LOCUS       DAVMEI010000229         1433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.233, whole genome shotgun sequence.
ACCESSION   DAVMEI010000229 DAVMEI010000000
VERSION     DAVMEI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.233"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            10..180
                     /locus_tag="VYQ24_001944"
     CDS             10..180
                     /locus_tag="VYQ24_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1969037.1"
                     /translation="MLESDSQLTYYWTFLSGKAEKQGITLYHHDQCRSGSVVQEFLGD
                     YSGYVHCDILRQ"
     gene            275..704
                     /locus_tag="VYQ24_001945"
                     /pseudo
     CDS             275..704
                     /locus_tag="VYQ24_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            775..933
                     /locus_tag="VYQ24_001946"
     CDS             775..933
                     /locus_tag="VYQ24_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV1969038.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1026..1433
                     /locus_tag="VYQ24_001947"
                     /pseudo
     CDS             1026..1433
                     /locus_tag="VYQ24_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          452 a          256 c          321 g          404 t
ORIGIN      
        1 tcttataggg tgctagagag tgatagtcag ctgacttact attggacttt tttgtcaggt
       61 aaagcagaga aacaagggat tacgctttac caccatgatc agtgtcgaag tggttcagta
      121 gtacaagaat tcctaggaga ttattctggc tatgtgcatt gtgatatttt gcggcagtaa
      181 cttaggactt tagtcctcta gttctgccta tgcgatagca gtccaaggtt taggagcaag
      241 gcgacgctaa gcttggtaaa cttcgaaccg ctcgtctgct tatcgtcaac tggaagaagc
      301 tgaacttgtt ggatgttggg cgcatgtgag aaggaagttt tttgaagcgc ccccccaagc
      361 aagcggataa atcatcctta ggagctaaag gtttagctta ttgtgatcag ttattttcct
      421 tggaaagaga ctgggaggct ttgccagctg atgaacgact acagaaacgt caagaacatc
      481 tccagccctt aatggaagac ttctttgctt agtgccggcg tcagtcagtt ttagcaggtt
      541 caaaactagg aagggcaatt gaatacagcc tcaagtatga agaaaccttt aagaccattt
      601 tgaaagacgg acatctggtc ctttccaata atctagctga acgcgccatt aaatcattgg
      661 ttatgggacg gagtaaaaga gtccagtgga ctcttttagc ctaagctcag tttaaaaaag
      721 cgagggtggt tattttctca aagttttgaa ggagctaaag caagagctat tattatgagt
      781 ttgttggaaa cagctaaacg tcatcaatta aatagcgaga aatatctatc ctatcttcta
      841 gaatgtcttc caaacgagga aactctcgta aacaaagagg ttttagaggc ttatttacca
      901 tggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg aactatccgt
      961 gagttctcca gtctggagat ttttcaatag acttcctgcg aaacaaaata tggtataata
     1021 gttctatgaa tgatgaagca agtaaacaac taaccgatgc acgatttaag cgtcttgttg
     1081 gtgttcaacg cacgactttt gaagagatgt tagctgtatt aaaaacagct tatcaactta
     1141 aacacgcaaa aggtggacga aaacctaaat taagtctaga agaccttctt atggccactc
     1201 ttcaatatgt gcgagaatat cgaacttatg aacaaattgc ggctgttttt ggtattcacg
     1261 aaagcaactt aatccgtcgg agccaatggg ttgaagtaac tcttgttcaa agtggtgtta
     1321 cgatttcaag aactcctctc agttctgagg acacggtaat gattgatgcg acggaagtaa
     1381 aaatcaatcg ccctaaaaaa agaattagcg aattattctg gtaaaaagaa att
//
LOCUS       DAVMEI010000230         1422 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.234, whole genome shotgun sequence.
ACCESSION   DAVMEI010000230 DAVMEI010000000
VERSION     DAVMEI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.234"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            471..1331
                     /locus_tag="VYQ24_001948"
     CDS             471..1331
                     /locus_tag="VYQ24_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEV1969039.1"
                     /translation="MKYGKIFKKFRESRGLSLKDVAKSGLSSSHLSRFEHDEADLTIS
                     KFLLALDAIHMPIEEFVYAVRDFRRDDFNELLEKIRYLVSKCDIDGMKKLLISQIEKK
                     DKKEPFDSFNTILLKIRLQDLLEEILVTDQEIGIISDYLFSVEYWGYYELLLFMNTID
                     VLNHKTMMVLAKEMCHRSEFYREIPNNRRLLATMLLNAYITCIERNELMDALYFEKQL
                     KYCNFSETEMYEKLVLHYTKNLYDLKKNNNRYAVLEMRKCIATMKLVDSNQTALKFEN
                     HLQKVLGDLA"
BASE COUNT          509 a          193 c          256 g          464 t
ORIGIN      
        1 aaataagttt ttctcttgca tttgtagctc atattcgtta taataatggt gtaaatgagt
       61 ggtcggctca tggtcaatct gatagtaatc ttggacataa gggccaagcg gtggcggaca
      121 ccagaacgaa atccgatagc aatcttggac gtaaggacca agcggtggca gacaccagaa
      181 taaaccgaca aaaatagtag taatgctata taaaactagg gagagggtga ctttttatcg
      241 ctcttttttt cattgaatta aaaaaattct agtgactttc atatcactag aatttaatgc
      301 atttcactaa cctactccga taacgataat actttcaagt attacaaata acaaactaaa
      361 ccatgctttt ttattcatga taaatacctc ttttcgtttt cttatatgat ataatatttt
      421 taaagattgt ggagaaattt cccttatacg caaattagga ggtaactgga atgaaatatg
      481 gaaaaatttt taaaaaattt agagaatctc gcggtttgtc tttaaaagat gttgcaaaat
      541 caggtctttc aagttcccat ctctcaagat ttgaacatga tgaagctgat ttaaccatca
      601 gtaaatttct tttagcctta gatgcaatac acatgcctat cgaagaattt gtttacgctg
      661 tacgtgattt ccgtagagat gactttaatg aacttttaga aaaaattcgg tatttagtct
      721 caaagtgtga tatagacgga atgaaaaagc tattaattag ccagatagaa aagaaggata
      781 aaaaagaacc attcgattca tttaacacaa ttttgcttaa aataagatta caggatttgt
      841 tagaagaaat tttagtaaca gaccaagaaa ttggtattat cagtgattac ttgttttctg
      901 tagaatactg gggatactat gaattgctac tgtttatgaa tacaatagat gttttaaatc
      961 ataaaacaat gatggtttta gccaaagaaa tgtgccatcg ttcggagttt tatagagaaa
     1021 ttcctaataa tagacgctta ttagcaacta tgttgctcaa tgcttatatc acctgtattg
     1081 aacgcaatga attgatggat gctctttatt ttgaaaaaca gctaaagtat tgtaactttt
     1141 cagagactga aatgtatgaa aagctagtgc ttcactatac taaaaattta tatgatttaa
     1201 agaaaaacaa taatcgatac gctgttttgg aaatgagaaa gtgtattgca accatgaaat
     1261 tggtagatag taatcagacg gctttaaaat ttgagaatca tttacaaaaa gtgttaggtg
     1321 acttggcgta aattgcgtat aagggaaaga aaatagaaat gaacataatt gtgttatgat
     1381 attaatgtca tcgatgatac agttttttac taactgggag tt
//
LOCUS       DAVMEI010000231         1419 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.235, whole genome shotgun sequence.
ACCESSION   DAVMEI010000231 DAVMEI010000000
VERSION     DAVMEI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.235"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..432
                     /locus_tag="VYQ24_001949"
                     /pseudo
     CDS             1..432
                     /locus_tag="VYQ24_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            599..799
                     /locus_tag="VYQ24_001950"
     CDS             599..799
                     /locus_tag="VYQ24_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969040.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            824..1241
                     /locus_tag="VYQ24_001951"
                     /pseudo
     CDS             824..1241
                     /locus_tag="VYQ24_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          485 a          291 c          292 g          351 t
ORIGIN      
        1 ataatccaga cgcttatttg actgaaatag cttctgaatt tggctgtcat ccaactacca
       61 tccactatgc gctcaaagct atgggctaca ctcgaaaaaa agaaccacac ctactatgaa
      121 caaaacccag aaaaagtagc cttatttctt aagaatttta atagtttaaa gcacctagca
      181 cctgtttaga ttgatgaaac aggatttgat acttattttt atcgagaata tggtcgctca
      241 ttaaaaggtc agttaagaag aggcaaagta tctggaagaa gatatcagag gatttctttg
      301 gttgcaggtc taacaaatgg tgaattaatc gctccaatga cttacgaaga gacgatgacg
      361 agcgactttt ttgaagtatg gtttcagaaa tttctcttac caacattaac cacaccatcg
      421 gttattatag taaaatggaa taagaacagg acaaatcgat caggacaatc aaatcaattt
      481 ctagcaatgt tttagaagtc catatgtact attctagttt caatatacca taactgctcc
      541 tagtcaacct gcaagtcctg tcccagctcc aagctcagct ccgactcaag atcctagagt
      601 ggtagtaagc gcttcggcta gctcaacgag cacacaagct caagagcaag ttgacaagtc
      661 tgaacttcgt gccttgagtc aagagttaga ccaacgcttg aaagctttgg caacagtatc
      721 tgatccgaaa atcgatgcaa ccaaggctgt cctcctagat gctcaaaaag ctccggaaga
      781 tagtgccttg acagagtaag gtctgcatac tgcagtagag tctgtgaaga ctgccttgga
      841 ctctctcaaa gatgtgatag caaatgctag cgacagcaag cctacacagg acaagaagga
      901 tgcaaaacaa ggaacggaag atagtaagga ttcagataag atgactgaaa caaactcagt
      961 tccagcagga gtgattgtgg tcagtctact tgccctccta ggcgtgattg ccttctggct
     1021 aattcgccgt aagaaagagt cagaaatcca gcaattaagc acggaattga ccaaggttct
     1081 aggacagcta gatgcagaaa aaagcggata aaaaagtcct tgccaaagcc caaaaccttc
     1141 tccaagaaac ccttgatttc gtgaaagaag aaaatggctc agcagagaca gaaactaaac
     1201 tagtagagga gcttaaagca atccttgaca aactcaagta aaacatgatt tatgaaatgt
     1261 cagacctaaa agacattttc gttcacaaaa gcagcgacga gtaatccttg tcgttgcttt
     1321 ttggctattt ttggggataa gatccgcctt gactagcaaa agaaaagatt ggtccaatag
     1381 tgcccgatac gattttgcgt agcagaaagc ttacaaata
//
LOCUS       DAVMEI010000232         1379 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.236, whole genome shotgun sequence.
ACCESSION   DAVMEI010000232 DAVMEI010000000
VERSION     DAVMEI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.236"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(177..>1379)
                     /locus_tag="VYQ24_001952"
     CDS             complement(177..>1379)
                     /locus_tag="VYQ24_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1969041.1"
                     /translation="SEMGFNVFRTSIAWSRIFPKGDEEEPNEAGLEYYDELFDELHAH
                     GIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYKDKVKYWLTFNEVN
                     SVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREINSEFKVGCMVLAM
                     PAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEHDINIEFAAEDAEL
                     LKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESSEWGWQIDPIGLRI
                     ILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYMKEHLLQVAEALQD
                     GVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRYKKMSFTWYKGVIE
                     SNGESLFK"
BASE COUNT          458 a          276 c          176 g          469 t
ORIGIN      
        1 ataaccaatc tatcctaagt taactattta taatcggctc tctgtaacaa aattaaatta
       61 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ctctttattt
      121 catatttaat gagatgttgc cttaaccgtg ataatatcta atcatcaata aacaaactat
      181 ttgaataagg attctccatt tgattcaatc actcctttat accaagtaaa ggacattttc
      241 ttatatcgat tgaatgtgcc atttccatca tcatttcgat caacataaat gagaccgtac
      301 ctcttagaaa gctgtgcagt ggacatagaa acgcagtcaa tacatcccca agacgtatag
      361 cccataattt ccacaccatc ttgtagagct tcagctactt gcaataaatg ttctttcata
      421 tactgaattc tataatcatc ttggactgtt aagttattaa attcatcttt tattagttga
      481 tctttagcac ctaatccgtt ttccactata aataatggaa tttgataacg gtcataatat
      541 ctatttaaaa ttatacgtag tccaattgga tcaatttgcc atccccactc agaagactct
      601 aaataaggat ttactaagcc accaataata ttcccttctc ctgaattata ctgtgttgga
      661 agagcagact gagtcacact catgtaatag ctaaaggata aaaaatctac ggtataattt
      721 tttaataact ctgcatcttc agctgcaaac tctatgttaa tgtcatgttc cttaaaatat
      781 ctttttgcat aattcggata ataacctcta acatgcacat ctgaaaatag ataatttaga
      841 ttctcatact catgagtcgc ccatacatct tttggatttg gagtcattgg ataagctggc
      901 atagctaata ccatacaccc caccttaaac tctgaattaa tctcacgagc aatttttgta
      961 accaaactcg aggcgactaa ttcatgatgt atagcttgat ataattcttg tttcgaaaga
     1021 ttctccttag gtatatctat tcctccacta gtaaatggta attccaaaac agagtttact
     1081 tcgttaaatg taagccaata tttaacttta tctttatacc tttctaaaac tgttcgagca
     1141 aatttttcat aaaaatgaat cattctccta tcaacccatc catgatattt tcttgctaaa
     1201 tataatggag tctcatagtg tgaaagagtt acaagtggct ctatcccgtg agcatgtagt
     1261 tcatcaaaca attcatcata atattccaaa ccagcttcgt taggttcttc ttcatctcct
     1321 tttggaaaaa ttctactcca tgcaatagaa gtacgaaaaa cattaaagcc catttcaga
//
LOCUS       DAVMEI010000233         1352 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.237, whole genome shotgun sequence.
ACCESSION   DAVMEI010000233 DAVMEI010000000
VERSION     DAVMEI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.237"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            104..>1352
                     /locus_tag="VYQ24_001953"
     CDS             104..>1352
                     /locus_tag="VYQ24_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEV1969042.1"
                     /translation="MNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPN
                     ELHKLIEKIAPKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGD
                     FSKKNIVLKEDDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNT
                     FKLDAKNAYFYTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLE
                     VLSENRSQDVNNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQ
                     LRFEIDGHEELRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKA
                     KFRELANIAFRQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQIIN
                     SVTGDEKKIIEFFRDSILNFNFLY"
BASE COUNT          505 a          161 c          242 g          444 t
ORIGIN      
        1 ttctattaaa gaaaattcct tagttttgtt tgttggtgca ggaatatcag ctaattctaa
       61 tctaccaact tggggagaat tgattcaatc attaaaaaaa gaattgaaca taccagaaga
      121 aagaacagat agtccactga gaattgctca gtattattat gatacttttg ggaaaaatca
      181 gtacacaaag aagatagaag aaattttctt taagaaggga ttgagtaaac caaatgagtt
      241 acataaatta atagaaaaaa ttgcaccaaa acatattatt acgacaaact atgattcact
      301 tctggagagt caatttgaaa gtggcttgtt aaagtacaat gtagtcgctg aggataaaga
      361 catcccttat acgagttcgg aaagatactt gattaaaatg catggtgact tcagtaagaa
      421 aaatattgtt ttgaaagaag atgattattt agactatcat ttgaacttcc caatgatttc
      481 tactttaatc cagtcattga taatgaatca tactctattg tttgtggggt attctctaag
      541 tgattcgact tttaattcaa tttttagaat gattcaaaat acatttaaat tggatgctaa
      601 aaatgcttat ttttatactc ccgaggaacc gtctatgatc attcgagagt attataagaa
      661 gcaaggaatt ttcattatct ctaatgaaga gaatctaggt caggagacat cagaaaaaca
      721 gaacaaattg tattgcagga caaaagattt tttagaggtt ctatcagaaa accgaagtca
      781 agatgtaaat aatgctgatg atttatggaa tcaactggct tttttagata gacttagttt
      841 tattgatgcg aaagattttt ctagatattc tgatttaaag aaaagggctt taaactggga
      901 tgatgagtat tgctggtttg gaaatgatca attaagattt gaaattgatg gtcatgaaga
      961 attacgaatt atggtatcta aaaaatcttt attaaatcgc tttctagata tggagattgg
     1021 tgagcctagg gatttaaagg ggaatcgatt cttaagtaag gcttttaagt tgtatgaaga
     1081 aaaacaatat tctttagcaa aagcaaaatt tagagaacta gctaatatag catttcgtca
     1141 gaaagactat tttaatttct tagtttgtga atttaatttt cagcaaattc aaataattga
     1201 tagatacgaa aaaacaccta gttatgcaga gccactttat gatggagaat taagcgaatt
     1261 aacagaacaa attattaact cggttacagg agatgagaaa aagattatag aattttttag
     1321 agattcaatc cttaacttca attttttata ta
//
LOCUS       DAVMEI010000234         1351 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.238, whole genome shotgun sequence.
ACCESSION   DAVMEI010000234 DAVMEI010000000
VERSION     DAVMEI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.238"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(139..327)
                     /locus_tag="VYQ24_001954"
     CDS             complement(139..327)
                     /locus_tag="VYQ24_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969043.1"
                     /translation="MRLGGSLGQRRENEIFFGLLAILFIKPIIGIVKFFWMIISFAVQ
                     LLFYKILFKILDWLFKLI"
     gene            complement(287..469)
                     /locus_tag="VYQ24_001955"
     CDS             complement(287..469)
                     /locus_tag="VYQ24_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969044.1"
                     /translation="MNPIKAFDKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIRPEERE"
     gene            complement(471..1250)
                     /locus_tag="VYQ24_001956"
     CDS             complement(471..1250)
                     /locus_tag="VYQ24_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969045.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYAF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLCAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLKIFGVNLTKGGLAKGIGKFIPILGGIIAGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          443 a          292 c          199 g          417 t
ORIGIN      
        1 gcaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg attttactgc
       61 ttatattata ccatatggag agagattaaa aaactagcag tggagttcct gctagctctc
      121 tgcgacttgg attaccatct agataagttt aaagagccaa tccaatatct taaataatat
      181 cttgtaaaac agcaattgaa ctgcaaagga aatgatcatc caaaagaatt tcacaatccc
      241 aataatcggt ttgataaaaa gaatagcaag aagaccaaaa aaaatttcat tctctctcct
      301 ctggcctaat gagccaccga gccgtatcca ttagcttatc tgcaataaaa agttttccca
      361 gaccaacaac gacatggtca gctttcttta tcgttttcat cacttttaca ccttgcacgg
      421 tcaaaaagta attaccataa attttatcaa aagctttgat aggattcata ttactctcct
      481 ttaatgattt cagcctcttt tcggattgtt tcaacatctt cttgatattg aacttcacta
      541 tagttgacta gcttggataa ttctttctgc aagctttccc ccattggttt catagttgca
      601 aaggttaaac cacctgcaat gataccaccc aagataggaa taaatttccc tattcctttg
      661 gccagccctc ccttggtaag attcacacca aatattttta agactttttt caaaataggg
      721 taccaaagcg tctttgttaa agctttatta ggcactgttt tcattacctg tttggcaatt
      781 gttataccac cagcacatag caaagcagcg gttccattca cccctaacat tacgcctaga
      841 taaagcaaga gggtattttg agcatcttca ctcaactcct cgcgtgaagc ccaaagatcc
      901 tcataaccat aaatataacc taattcttga gccaatttca gagagaaagc ataaaattga
      961 gccacatcag ctggaatggt aattgccata gcaagccctc cagctaatcc tgccaaaaca
     1021 gaagtcccac tcgctaataa tacattgtcc cgaatacaag cattagctac acgatctaat
     1081 atttcttgag atagaagagt cgttgtacct tgttccaata aggtaggaat atcttttgga
     1141 tccaattctt tggaaaactt atccactaaa aatttcgaac gatcaacctt aacaacaggt
     1201 agtttcacca cttgttgcaa tacttgcata gctaagtctt gttcagccat atacaaactc
     1261 cttatttctt ttttacacct tatcatatta tatttcctac aaaaaaagca ttttctaatt
     1321 agataacatt aaaaaataca gaaccccttt t
//
LOCUS       DAVMEI010000235         1315 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.239, whole genome shotgun sequence.
ACCESSION   DAVMEI010000235 DAVMEI010000000
VERSION     DAVMEI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.239"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(708..1280)
                     /locus_tag="VYQ24_001957"
     CDS             complement(708..1280)
                     /locus_tag="VYQ24_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969046.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT          399 a          224 c          221 g          471 t
ORIGIN      
        1 gtaggaaaga aggtgttttt atgatataca cacttaaatt ggtgttgttt attacctttc
       61 ttgtaataag cttgttacct gataagattt ttggaaaaaa taaaaaaatt tggaaaatag
      121 tttttgcaat attgacggca gtggcagcat tgtcatttat gtactaagtt attttaagaa
      181 tgtagggaaa taaaccctac attcttttta gttttttctg ttttctaaat tctatttatc
      241 caagcgcttc aacatttctt gcttcttcgc ttcaagttct gcacgctttt cttcgatttc
      301 ggcatgtttt ttctcgagtt cagaacaact tgcatcattg ctaaattctt ttcgccatca
      361 ggagataggg tgagtcgaca tgtctattac tcacccaaag cagtcctaca aagcaggaat
      421 tttctgttac ttttttggaa atagtaacgt ttatacagct ttgacacttc gtatcaaagc
      481 gccaaacaca ctccgaggga tttacagaaa gcagaaaagg aatgatctgg tataagatca
      541 ttccttttct ctctttttct ttaagtaatt atatacaatg tacgacgaag tcgtcattgc
      601 aatgctgatc caccacctaa agggaacttt aaacaacatt gataagataa agaatataaa
      661 caacgaaaat acgttatacc caattaattt tattgtatat ctcatgatta aaagttaatc
      721 cttccgttgt taggaatggc atcattttta tcccataatt gtgctaaata agtccccggt
      781 gataataaat tcatagcgaa ttctaaagca acatcattta caaaccaact acctagatat
      841 ctagaaattg ctgaacgaat agcacttttt gctgcatgtt ttccttttac tttaattaga
      901 tttgcaaggc ctgcagtagt tcctcctaat gctaaagcta ttgcagtatc taatagagca
      961 cccatttgat taactgtaat accttgccaa gctgctctaa atggagagta tgtaggtggg
     1021 attgtataat cgccttgtaa ttgtcggtta attacttctt tgatccattg ttgtgagacg
     1081 tctggatgaa aagattggat ttcgtttgca agtgtattga tttgttcttc tgttagagaa
     1141 gtgacaggtt gaagttccat atttgtttca atttgtgata cttgttcaga agcgtataca
     1201 gctgaaacac ttggaatcgc tgatacaatt aacacaattg acgtcaaaaa aaccgaaata
     1261 aatttcatta atttgttcat gagcttttct cctttttatt tgtatctgct tacat
//
LOCUS       DAVMEI010000236         1291 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.240, whole genome shotgun sequence.
ACCESSION   DAVMEI010000236 DAVMEI010000000
VERSION     DAVMEI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.240"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..731)
                     /locus_tag="VYQ24_001958"
     CDS             complement(<1..731)
                     /locus_tag="VYQ24_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969047.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINNP"
     gene            complement(1046..>1291)
                     /locus_tag="VYQ24_001959"
     CDS             complement(1046..>1291)
                     /locus_tag="VYQ24_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969048.1"
                     /translation="GIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVV
                     INEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
BASE COUNT          467 a          214 c          176 g          434 t
ORIGIN      
        1 attggattat ttattttttt aaatcgctta acttctaata aatcaatttg ttcacgcaca
       61 ctctctgaaa gatatggctt gattaaactg atataagata aagccacgca tggcaactca
      121 gattttgatt gcaatttcca aatttcatga taatcctgaa gattaaactc acaattcttc
      181 ttagattcca gtattgttag tacctttgat tttaacagaa ataattgttg attctcaatt
      241 ctataaagtt tttgtactaa tgctatgata atagaaaatg aaataagagg caaaagcaac
      301 ggtttgtcat acacaaaagt aaagaaaaaa tagtatgaaa ttagtgttgt aaagtacaag
      361 aagtagtaaa atttaaacag attcttagtt gtttgactct ccttataaaa cactgctatt
      421 cctggactaa atcccattag ttggtgaaag agaattcgat agtatttcct agctcctata
      481 atgatcacaa atataaaata aaaataaatt ggtcttaatt tacaaaagat tgaggaaact
      541 agtaatagca aagtatgtaa gcattgagac aaaatataga ataacggtat cgccaataca
      601 aaggttaaga tggcatgaga gggagttcct aagatgtcca attttataaa tactgggctg
      661 acacaaaata tcgtgcttat aagcagtaaa attattaaaa ttgtgtcaaa aaaataacgt
      721 ttatttttca tttagtctac tttccattgt taaataatac tactgctaac accaagccaa
      781 cttagcacat tgcatcacct aatcctgcgc tacttatcca taatccttta atgtttctac
      841 taaaattatt ccaatttccc ccatcaagat agcttattta tcctagacaa tgcctactgc
      901 aacagtataa ataagattca tcagccatct tttctggcaa tcatatgggt agcctaatga
      961 tatttctagt tactcaaaaa tatctagcta tactagagtt atttctaatc cctaggacac
     1021 ttgttaatcc aaatgtaaca aattttcacc ccttaatagg actctttctg gaaaaaatat
     1081 aaggtctatc tttataaaaa aatctatcat attgggatgg cacgcatctg gaatttaaac
     1141 aagctataaa ttcatttata actactattt gtttagaggc aacagcatct tgtataccgc
     1201 cctcgaaaga accatcttgt aattccgaaa tatttttgtt taaatattgt tttgacaaat
     1261 ctcttaatgc ttcattatcg taaatgattc c
//
LOCUS       DAVMEI010000237         1265 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.241, whole genome shotgun sequence.
ACCESSION   DAVMEI010000237 DAVMEI010000000
VERSION     DAVMEI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.241"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            4..>1265
                     /gene="pcpA"
                     /locus_tag="VYQ24_001960"
     CDS             4..>1265
                     /gene="pcpA"
                     /locus_tag="VYQ24_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1969049.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTS"
BASE COUNT          485 a          182 c          206 g          392 t
ORIGIN      
        1 taaatgaaaa aaactacaat attatcatta actacagctg cggttatttt agcagcatat
       61 gtccctaatg aaccaatcct agcggatact cctagttcgg aagtaatcaa agagactaaa
      121 gttggaagta ttattcaaca aaataatatc aaatataagg ttctaactgt agaaggtaac
      181 ataggaactg ttcaagtggg taatggagtt actcctgtag agtttgaagc tggtcaagat
      241 ggaaaaccat tcacgattcc tacaaaaatc acagtaggtg ataaagtatt taccgttact
      301 gaagtagcta gtcaagcttt tagttattat ccagatgaaa caggtagaat tgtctactat
      361 cctagctcta ttactatccc atcaagcata aaaaaaatac aaaaaaaagg cttccatgga
      421 agtaaagcta aaactattat ttttgacaaa ggcagtcagc tggagaaaat tgaagataga
      481 gcttttgatt tttctgaatt agaagagatt gaattgcctg catctctaga atatattgga
      541 acaagtgcat tttcttttag tcaaaaattg aaaaagctaa ccttttcctc aagttcaaaa
      601 ttagaattaa tatcacatga ggcttttgct aatttatcaa atttagagaa actaacatta
      661 ccaaaatcgg ttaaaacatt aggaagtaat ctatttagac tcactactag cttaaaacat
      721 gttgatgttg aagaaggaaa tgaatcgttt gcctcagttg atggtgtttt gttttcaaaa
      781 gataaaactc aattaattta ttatccaagt caaaaaaatg acgaaagtta taaaacgcct
      841 aaggagacaa aagaacttgc atcatattcg tttaataaaa attcttactt gaaaaaactc
      901 gaattgaatg aaggtttaga aaaaatcggt acttttgcat ttgcggatgc gattaaactt
      961 gaagaaatta gcttaccaaa tagtttagaa actattgaac gtttagcctt ttacggtaat
     1021 ttagaattaa aagaacttat attaccagat aatgttaaaa attttggtaa acacgttatg
     1081 aacggtttac caaaattaaa aagtttaaca attggtaata atatcaactc attgccgtcc
     1141 ttcttcctaa gtggcgtctt agattcatta aaggaaattc atattaagaa taaaagtaca
     1201 gagttttctg tgaaaaaaga tacatttgca attcctgaaa ctgttaagtt ctatgtaaca
     1261 tcaga
//
LOCUS       DAVMEI010000238         1228 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.242, whole genome shotgun sequence.
ACCESSION   DAVMEI010000238 DAVMEI010000000
VERSION     DAVMEI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.242"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            40..1214
                     /locus_tag="VYQ24_001961"
                     /pseudo
     CDS             40..1214
                     /locus_tag="VYQ24_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
BASE COUNT          447 a          118 c          246 g          417 t
ORIGIN      
        1 atcaaatatc ataatgcgtc gtttaaggag atggttacaa tgcaatatag ttgtggaaaa
       61 ataaatatta atatccctga tggatatgga gacattaagg atatagtgtt ttcggctcat
      121 attatagtta ggtataataa tggacattgt ggaggtatag atccgcatat aattggactt
      181 tgtaaaaagc agatcagaag gatgtctcta tatcctattt tgataatcgt atctagagat
      241 tcaaaggtta ttgatgatta taaaaattta gatattgcct atgttgattg tactcaatgt
      301 tcaaataatt ttgaaactgc cttacacgtt aaaaatattt taaaattatt aaaaatccaa
      361 cttatacact gtcatggcta ctcaactaat tattttttat atatgttaaa aaaacttgac
      421 aagaatggat ttggaaaggt taagacagtg ataacatgcc atggatgggt tgaatataac
      481 ttaaaaaaga aattcttgac ttattttgat ttttggacat attctatggg agatgctttt
      541 atttgtgtat ctgaaactat gaaaaaaaga ttggagagta taataaaaaa ataagaagat
      601 agttgcaata aataacggaa ttaatgtatc aaatagcgat ttagatgtag taggtgttca
      661 agactttaaa aaagaatttt gtattccaaa taacaaaaaa atcatttgtt atgttgaaag
      721 gttggatcca gaaaaaaggc cggatagatt ccttgaattt gcagaaaaat tatttttagt
      781 cagagaagat gtgattttta ttatggctgg aaatggtagt atgtgggctg ctttgaaaga
      841 aaaaatttgc catttgaaat gtagagataa tttcaggtta cttggtgaaa tctatccagc
      901 tactatagtt taccaaatat cggatttatt gtatattcct tctgatacag aaggaatacc
      961 tatgtgtgtt ttagagtcaa tgtcacaagg aactccggtt ttagctagta atgttggcgg
     1021 gttaagtgaa attattgaac atagggttga tggattttta tttgagaagg aagatgttga
     1081 gggagtgtgt gcttgtgcta attttttact caatgattct gagtatttga aatatatagg
     1141 tgagaatagt aaatcaaaaa taagaaaaca tgagagtata tgatgaatta ttagagaaga
     1201 gtagtcatgg ataggaatat agatcaag
//
LOCUS       DAVMEI010000239         1204 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.243, whole genome shotgun sequence.
ACCESSION   DAVMEI010000239 DAVMEI010000000
VERSION     DAVMEI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.243"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..195
                     /locus_tag="VYQ24_001962"
                     /pseudo
     CDS             1..195
                     /locus_tag="VYQ24_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856116.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
     gene            173..>1204
                     /locus_tag="VYQ24_001963"
     CDS             173..>1204
                     /locus_tag="VYQ24_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1969050.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRE
                     RIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQ
                     MEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVAPLP
                     LGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQ
                     LKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSR
                     IDKVRYMFNK"
BASE COUNT          394 a          205 c          247 g          358 t
ORIGIN      
        1 taaattttat tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat
       61 tgaatataca aaaaaaagac aacgtcttgc atatatacgc ctttatagtg gagtactaca
      121 tttacgagat tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac
      181 ttcaataaat ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt
      241 gcaaaatgag tttttgaagt taaatagtgt tcttggagat acaaagctat tgccacagag
      301 agagagaatt gaaaatcccc tccctctgct gcaaacgact gttgaaccga gcaaacctca
      361 acaaagggaa atgttacttg atgcactttt agaaatctcc gacagtgacc cgcttctgcg
      421 atattatgtg gattctgcga cacatgaaat catactttct ttcttaggga aagtacaaat
      481 ggaagtgact tgtgctctgc tgcaagaaaa gtatcatgtg gagatagaaa taaaagagcc
      541 tacagtcatt tatatggaaa gaccgttaaa aaaagcagag tataccattc acatcgaagt
      601 tccaccgaat cctttctggg cttccattgg tctatctgta gcaccgcttc cattagggag
      661 cggagtacag tatgagagct cggtttctct tggatactta aatcaatcgt ttcaaaatgc
      721 agttatggag gggatacgct atggctgtga acaaggattg tatggttgga atgtgacgga
      781 ctgtaaaatc tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt
      841 tcggatgctt gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt
      901 agagccatat cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa
      961 cgatgctcct aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat
     1021 tcttagtgga gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt taactttctt
     1081 tacaaatgga cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga
     1141 acctgtttgc cagccccgtc gtccaaatag tcggatagat aaagtacgat atatgttcaa
     1201 taaa
//
LOCUS       DAVMEI010000240         1199 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.244, whole genome shotgun sequence.
ACCESSION   DAVMEI010000240 DAVMEI010000000
VERSION     DAVMEI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.244"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..251
                     /locus_tag="VYQ24_001964"
     CDS             <1..251
                     /locus_tag="VYQ24_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1969051.1"
                     /translation="LKIEIVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSD
                     LIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            251..1012
                     /locus_tag="VYQ24_001965"
     CDS             251..1012
                     /locus_tag="VYQ24_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1969052.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          440 a          167 c          242 g          350 t
ORIGIN      
        1 ctttgaaaat tgaaattgtg aaagaaagag gtgctaatct attatctcga ctctatcgtt
       61 atcaagatag tcagggaatt agcattgatg atgaatctaa tccttggatt ttaatgagtg
      121 atgatctatc tgacttgatt catacgaata tctatttagt agaaactttt gatgaaatag
      181 agagatatag tggctatttg gatggaattg aacgtatgtt agagatatct gaaaagcgga
      241 tggtagctta atggaaatcc aagattatac tgatagtgaa ttcaaacatg ctttagcaag
      301 gaatcttcgt tcactgacaa gaggaaaaaa gtccagtaag caacctatag cgattttgct
      361 tggagggcaa agtggtgccg gtaagactac aattcatcgt attaaacaga aagaatttca
      421 aggaaatatt gttatcatag atggtgatag ttttcgttct cagcatccac actatttaga
      481 actgcagcaa gaatatggca aagacagtgt agaatatacc aaagattttg caggaaaaat
      541 ggtagagtct ttagtaacaa aattgagtag tttgagatac aatcttttga tcgagggaac
      601 tttacgaaca gttgatgttc caaagaaaac agcacaactc ttgaaaaata agggatatga
      661 agtacaattg gccttaattg cgacaaagcc tgaattgtcg tatctaagta ctcttatccg
      721 ttatgaagaa ctgtacatta tcaatccaaa tcaagcacgc gcaactccaa aagaacatca
      781 tgatttcatt gtaaatcatc tagttgataa tacacgacaa ttggaagaac tggctatctt
      841 tgaaagcatt caaatttacc aacgagatag aagttgtgta tatgattcaa aagaaaatac
      901 aacttcagca gcagatgttc ttcaagagtt actctttggg gagtggagtc aggtagagaa
      961 ggagatgttg caggtggggg aaaagagact taatgaatta cttgaaaaat aaacaattga
     1021 tatttttagg agaatagaaa tgagagggtt taataacaag ataaagtctg tttatcaaga
     1081 actaacaaat tccaaagaga aattcggtag ctttcacaag actttaattc atttgcatac
     1141 acctgtttct tatgattaca agctattttc taattggact gcaacgaaat atagaaaaa
//
LOCUS       DAVMEI010000241         1187 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.245, whole genome shotgun sequence.
ACCESSION   DAVMEI010000241 DAVMEI010000000
VERSION     DAVMEI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.245"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            75..1187
                     /locus_tag="VYQ24_001966"
     CDS             75..1187
                     /locus_tag="VYQ24_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1969053.1"
                     /translation="MQMEIKAYNLSKDIKDDLYKKMEDSERVHLKAEVTTILTLSISS
                     IFSFISLAVVIFVGVNLIINKEINSLYLIGYLLAAMKIKDSLDASKEGLMEIFYLSPK
                     IERLKEIQNQDLQEGDDYSLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVG
                     ASGCGKTTILKLISRLYDYDKGQILINGKDIKEISTESLFDKVSIVFQDVVLFNQSVM
                     ENIRIGKQDASDEEVKRAAKLANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIAR
                     AFLKDAPILILDEITASLDVNNEKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVL
                     QNGRVESEGKHEELLQKSKIYKNLIEKTKMAEEFIY"
BASE COUNT          482 a          143 c          226 g          336 t
ORIGIN      
        1 atcaggttaa tggacagaat agatattatg atgtcttaag aaaaaactca gaaagctttc
       61 aagaaaatat cgaaatgcaa atggagatta aagcatataa tttatcgaag gatattaaag
      121 atgacttata taaaaaaatg gaagatagtg agagagtaca cttaaaggcg gaagtaacta
      181 caattttaac tttgtctata tcttcaatat ttagctttat atctcttgct gttgtgatat
      241 ttgtcggcgt aaatctaatt attaataaag agataaattc tctctacctt ataggatatt
      301 tactagctgc tatgaagata aaagactctt tagatgcatc taaagagggc ttgatggaaa
      361 tattttattt atcgcccaaa atagaaagat taaaagaaat tcaaaatcaa gatctacaag
      421 aaggcgatga ctatagctta aaaaaatttg atattgatct aaaagatgtt gagtttgcct
      481 acaataaaga cgcaaaagtt ttaaatggtg taagttttaa agctaagcag ggagaggtca
      541 ctgctttggt aggtgcaagt ggctgcggta aaacaactat cttgaaactt atatcaagac
      601 tttatgatta tgacaaggga caaatcttaa tcaatggcaa agatataaaa gaaatatcaa
      661 cagaatctct ttttgataag gtctctatag ttttccaaga tgtggttctc tttaatcaaa
      721 gcgttatgga aaatattaga atcggaaagc aagatgcaag tgacgaagag gttaaaagag
      781 cagcaaaact tgcaaattgc acagatttta tagaaaaaat ggataaaggt ttcgatacag
      841 ttattggtga gaacggagct gagctatcag gaggagaaag acaaagatta tcaatagcca
      901 gagccttctt aaaagatgcg ccgatattga tcttagatga gataacagca agccttgatg
      961 ttaacaacga gaaaaagatt caagagtctt taaataattt agttaaagat aaaactgttg
     1021 taatcatttc acatagaatg aaatccatag aaaatgcaga caagatagta gttcttcaaa
     1081 acggaagagt agaaagcgaa ggtaagcatg aagagctttt acaaaaatca aaaatttaca
     1141 aaaatttaat agaaaagaca aaaatggcag aagaatttat ttattag
//
LOCUS       DAVMEI010000242         1173 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.246, whole genome shotgun sequence.
ACCESSION   DAVMEI010000242 DAVMEI010000000
VERSION     DAVMEI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.246"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            48..398
                     /locus_tag="VYQ24_001967"
     CDS             48..398
                     /locus_tag="VYQ24_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1969054.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDILAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            613..729
                     /locus_tag="VYQ24_001968"
     CDS             613..729
                     /locus_tag="VYQ24_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1969055.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            740..930
                     /locus_tag="VYQ24_001969"
                     /pseudo
     CDS             740..930
                     /locus_tag="VYQ24_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          392 a          232 c          240 g          309 t
ORIGIN      
        1 gttattcaca tcttatttca actcactata gaaggaggaa taggaggatt ctcagacatc
       61 cgggcatcag cccaactaat gatttgattg ctaagaaaat attcagcaat ccagaaatca
      121 cttgtcaatt tattcgcgat atgctggact tgccagcaaa aaatgtgacc attttggagg
      181 gaagcgatat tcacgtatta ctctccatgc cttactcggt gcaggatttt tataccagta
      241 tagacatctt ggcggagttg gataacggta ctcaagtaat tattgagatt caagtccatc
      301 atcagaattt tttcatcaat cacttgtggg cttacctgtg cagtcaggtt aatcaaaatc
      361 ttgaaaaaat tcgtcagcga gaaggtgata ctcactagat ctacaaacac atcgctcctg
      421 tttacgtcat tgctatcgtg gatagtaatt atttctcaga tgacctggct tttcatagct
      481 ttagtatgcg cgaagacaca acaggtgagg tattggcgat taccaacaat ggacaggaaa
      541 accatctggt taagatggca ttcttggaat taaaaaatac agagaaacca gcaaagacaa
      601 ggttcgcaag ccatggttgg agtttttcgg caacaagccc tttacccagc aaccgcaacg
      661 agccattacc caagcaaatc aactgctgga ctacaagagc tggtccgagg aggacaggaa
      721 aatgtttagt caactacata tgcgagaaga acaagtcttg ttagcacagg actatgcctt
      781 gaacaaggac tagagcgtgg gaaagttgaa ggaagggaag aaaggaaact ttttgccttc
      841 ctagacatag tacgccaagg tcttctgact tctgaggttg ccagccagca attaggtatg
      901 tcagtatctg aatttgaggc actgttgtaa aatggctcca taatatccat agtgggtaaa
      961 tcccctatgg atattatgga gcctattttg tgtagaaaaa aagtcccata tgacctataa
     1021 tgaaaagcga caaaacaact cattagaaag aatcatatgg aacaattaca ttttatcaca
     1081 aaattactag acattaaaga ccctaatgtc cagattttaa acatcatcaa taaggataca
     1141 cacaaggaaa tcatcgccaa actggactac gac
//
LOCUS       DAVMEI010000243         1162 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.247, whole genome shotgun sequence.
ACCESSION   DAVMEI010000243 DAVMEI010000000
VERSION     DAVMEI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.247"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..288)
                     /locus_tag="VYQ24_001970"
     CDS             complement(<1..288)
                     /locus_tag="VYQ24_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969056.1"
                     /translation="MNNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILE"
     gene            complement(285..617)
                     /locus_tag="VYQ24_001971"
     CDS             complement(285..617)
                     /locus_tag="VYQ24_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1969057.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICVALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGKIAYCIGYGALNRGCDISGNPYPWD
                     GFISATVR"
     gene            complement(673..>1162)
                     /locus_tag="VYQ24_001972"
     CDS             complement(673..>1162)
                     /locus_tag="VYQ24_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969058.1"
                     /translation="FWTIMDINHLVFNKTEFLFNQNKWEKEDSFESDLTKTLKDKIQK
                     SELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCTGLVELLLYEMSI
                     SNNNNWSNKVDKIRIHLIRINQLDFFYYTSWLRQNFDFCMNREYHKMKSRKLLLSNKK
                     IV"
BASE COUNT          399 a          217 c          154 g          392 t
ORIGIN      
        1 ctctaggata aaaacactta ttaatacggt ataagtcaaa aaactttctg ggaaaaactt
       61 attgattaaa atggggaaac tcattaacag aatcgaattt atggctagac agatagatac
      121 tattttattt ttcacagaat atagaggtag tgatactatt gagtagataa aagctaaaaa
      181 aataagcaaa aaatactccg tgtcatttat aatcgccctg aatgataaaa atatatacac
      241 ccaacatacg ataatatgta tttttcgaat cttatctaca ttattcatct tactgtcgca
      301 gatataaatc catcccaagg ataagggttc ccgctaatat cacaacctct attaagagca
      361 ccatatccaa tacagtaagc tatttttcct gctgctgcac caataagcca gcctccaaga
      421 cctccaacct taatatagtt tattagtttt ttggttaaaa ctgcagctcc agctaatgct
      481 ataaaagttc ctgaactcat agctaaagct acgcaaattc cttgacaagc acgcttgctt
      541 aaataagctc ccccatcaag atacatcatt tcttcttcat caatcacaac ataattattt
      601 ggtaatacaa gttccatttg ttttctcctc cttagttgaa attgtttcat ttgttttaca
      661 aataggacga acttaaacta tctttttatt actcagtaat aatttccgag atttcatttt
      721 atgatactct ctattcatac agaaatcaaa attttgcctt aaccaacttg tataatagaa
      781 aaaatctaat tgattgattc tgatgaggtg tatcctaatt ttatcaacct tgttggacca
      841 gttgttattg ttactaatag acatttcata cagcagtaac tcgacaagac ctgtacacag
      901 aagggtgtta tgagaatctt cgattttttt caaaatatcc tcaaaaagtt cactatccgt
      961 taaaaaggtg gattggttca tcgcatcata cagctgaaga gacattaagt cagaataact
     1021 tagctctgat ttttgtatct tatcctttaa cgttttcgtc aaatcgcttt caaacgaatc
     1081 ttctttttcc catttatttt gattaaaaag aaattccgtc ttattaaaaa cgagatgatt
     1141 gatatccatt atagtccaga aa
//
LOCUS       DAVMEI010000244         1142 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.248, whole genome shotgun sequence.
ACCESSION   DAVMEI010000244 DAVMEI010000000
VERSION     DAVMEI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.248"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            84..491
                     /locus_tag="VYQ24_001973"
     CDS             84..491
                     /locus_tag="VYQ24_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1969059.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VYQ24_001974"
     CDS             656..955
                     /locus_tag="VYQ24_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1969060.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
BASE COUNT          408 a          229 c          193 g          312 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact cctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tcaaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat tcaaaccaca tcagcgtcgc cttaccatac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttt attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag gcctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 ca
//
LOCUS       DAVMEI010000245         1141 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.249, whole genome shotgun sequence.
ACCESSION   DAVMEI010000245 DAVMEI010000000
VERSION     DAVMEI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.249"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            281..>1141
                     /locus_tag="VYQ24_001975"
     rRNA            281..>1141
                     /locus_tag="VYQ24_001975"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          335 a          211 c          339 g          256 t
ORIGIN      
        1 taacacgggt tcgaatcccg tacggactat ggtatgttgc gtcaggacca cttgatgaaa
       61 aaaagtttaa aaaagcttaa aaatcttcaa aaaagtgttg acaagtgaaa gtggctgtga
      121 tatactaata tagttgtcgc ttgagagaag caagtgacaa agacctttga aaactgaaca
      181 agacgaacca atgtgcaggg cgctacaaca taagttgtag tactgaacaa tgaaaaaaca
      241 ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga gtttgatcct
      301 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct gaaggaggag
      361 cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct gcctggtagc
      421 gggggataac tattggaaac gatagctaat accgcataag agtggatgtt gcatgacatt
      481 tgcttaaaag gtgcacttgc atcactacca gatggacctg cgttgtatta gctagttggt
      541 ggggtaaggg ctcaccaagg cgacgataca tagccgacct gagagggtga tcggccacac
      601 tgggactgag acacggccca gactcctacg ggaggcagca gtagggaatc ttcggcaatg
      661 gacggaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaagctc
      721 tgttgtaaga gaagaacgag tgtgagagtg gaaagttcac actgtgacgg tatcttacca
      781 gaaagggacg gctaactacg tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc
      841 cggatttatt gggcgtaaag cgagcgcagg cggttagata agtctgaagt taaaggctgt
      901 ggcttaacca tagtaggctt tggaaactgt ttaacttgag tgcaagaggg gagagtggaa
      961 ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccggtggc gaaagcggct
     1021 ctctggcttg taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc
     1081 ctggtagtcc acgctgtaaa cgatgagtgc taggtgttag accctttccg gggtttagtg
     1141 c
//
LOCUS       DAVMEI010000246         1108 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.250, whole genome shotgun sequence.
ACCESSION   DAVMEI010000246 DAVMEI010000000
VERSION     DAVMEI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.250"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(16..867)
                     /locus_tag="VYQ24_001976"
     CDS             complement(16..867)
                     /locus_tag="VYQ24_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1969061.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
BASE COUNT          358 a          148 c          195 g          407 t
ORIGIN      
        1 gagagttatt acaaattata ggtttgaaaa atcattaatt tctttttcga gtattttaac
       61 taatgcctct tctttggtta gtcgtaataa agttattcct ttatcaatca aatcggagtt
      121 atgttgtagt attcccaagc gtacttgaga gaatccaaga atatcgtata tttttaattc
      181 ttctgctaat accaaaagtt gtagggtgat tgtttcacat tctttgaata aatgatgttg
      241 aaaatataca gtggagagat tggctaaaaa agatgataaa aatgcttttg tttctcggaa
      301 gtatttatat ttctcgatgg ttttcataat tctatcaata agaaggggaa gattttcaga
      361 aggaaagaaa aagagaatgg ttccaagcaa tttcaaatca ctaatgtacc atgtatccat
      421 aggttcaaga tagtcccaaa tttcttcata caaatctttc aagacgtggt tgtttttgaa
      481 tcctttagtt cgtaactctg tgacaatttt tgtatgacga tagatatttt gaataggaac
      541 atcgtgatgt gtcttaagat atttttgaca tttctcagtg agttcaacca tttttctagt
      601 atcttgaaaa gtagacggat tttggctctc tacaataata tctcgccttt tactaggatg
      661 gtattcgttg catatatact tgaattctgc caagctcata tcaatttgtt caagaagaaa
      721 taccatgttt tcaaaactgg gaataacttt accgtgttca atatttgtta gagttgttcg
      781 atgtatgact tgtccacata catcttgttg ggtcaatcct tttgactttc gaatttcttt
      841 aaaaatttgt ccataatccc atctcatatt gttcctccta ggtaaatgtg aatattttct
      901 acatttataa aaaacgtaga aaaaggtggt attattatat caaaaaggag ggaaaaagtc
      961 atgggcttta aaaaatattt gaagaattta ccgaaaaact ctggattttt gatttggagt
     1021 tggattcaac ttatctggtt tgaaacatgg ttttggggat aaagaaatta gagtctatac
     1081 ataataagta aaggaggaat ttgtatga
//
LOCUS       DAVMEI010000247         1095 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.251, whole genome shotgun sequence.
ACCESSION   DAVMEI010000247 DAVMEI010000000
VERSION     DAVMEI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.251"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            338..>1095
                     /locus_tag="VYQ24_001977"
     CDS             338..>1095
                     /locus_tag="VYQ24_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1969062.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKG"
BASE COUNT          492 a          160 c          197 g          246 t
ORIGIN      
        1 tcatctaaag atgaaataac ataattgtcc cttggttaca agggcgcaat tatacaccct
       61 aaagggtgct tgcgtcctgc ggagtcaaac ccttgcaggg agcaacaacc attcgctttt
      121 taaaagtcaa gggtaaataa actcgctaag gctcgccctt gacttttaaa atttgaaatg
      181 aaagtaacaa ttaagccgac atactgaaac aacaaaaatt aattcagtag tcggcttttt
      241 ttattctatc atttcaaaac gctcattcac aaagaggata taaaaaaact attaagcctc
      301 cacctaaaac aacaaagaaa gaaaggaagt gataaaaatg gcagacagtt ttcatttttc
      361 agtaaacata atatcaagag gaaaaggcaa aagtgcagta gcaagtgcag catatataag
      421 tggagaaaaa ataaaaaatg aatgggacgg agtaacccat gactacacaa gaaaagaaaa
      481 aatattagta aaaaatataa tattgcctga tcatatacca aaagaattta atgataggtc
      541 taccttatgg aataaagtag aaatggcaga aaaaaattct aatgcacaac tagcaagaca
      601 attcataata ggactaccaa aagagttatc tttaagtgaa aataaaaacc tggttgaaag
      661 atatataaaa gaaaatttaa catcacaagg aatgatagta gattatgcaa ttcatgatga
      721 gagtcaagat aaaaatggaa atatccattg tcatataatg accataatgc gaccaataaa
      781 cgaaaaagga gagttcttag ccaagtcaaa aaaagaatat atcctagatg aaaaaggaga
      841 aaaagtatta aacaaaaatg gaaaaccaaa gacaagaaaa gtagaactaa caacctggaa
      901 cgatacaggc aatgtagaac aatggagaga aaatttttca gatctttgta acaaatattt
      961 agaaagagca ggagcagaaa aaagagtaga ccataggagt tttaaaagac aaaattcaga
     1021 ttatctaccg acaatccatt taggatcagc agcaagtgca atggaaagaa agggaataga
     1081 aactgacaag ggaaa
//
LOCUS       DAVMEI010000248         1092 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.252, whole genome shotgun sequence.
ACCESSION   DAVMEI010000248 DAVMEI010000000
VERSION     DAVMEI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.252"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            52..714
                     /locus_tag="VYQ24_001978"
     CDS             52..714
                     /locus_tag="VYQ24_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1969063.1"
                     /translation="MNEQLPDEMTLAKQFACSRMTIKKALDLLVSEGLIFRKRGQGTF
                     VLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRIIHFKLEFANEFLAEKLQVALQS
                     PVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDILQKSIYNYIEGKLGLHIASATKI
                     LRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTPFEYSISRHRYDLFEFNSFALRH
                     SS"
BASE COUNT          359 a          173 c          191 g          369 t
ORIGIN      
        1 ttatttattt acaatgagat tcgtgaaaag attctttgta ataaatatac catgaacgaa
       61 caattacctg atgaaatgac attagctaaa cagtttgcct gtagtcgaat gacgatcaaa
      121 aaagctttag acttgttagt ttctgagggc ttaattttta gaaaacgtgg gcagggaacc
      181 tttgttctct ctcgtggcag ctcaaaaaga aaattaatcg ttccagaaag agatatccgg
      241 ggactgacaa aaatatctga agatgctcat tctacaattg actcgaggat tattcacttc
      301 aaattagaat ttgcaaatga atttttagca gaaaaactac aggtcgcttt gcagagtcca
      361 gtttataata tttaccgcct gcgtattatt gacggtaaac cttatgttct ggaacaaact
      421 tatatgagta ccgatgttat tccaggtatt actgaagata ttttacaaaa atcgatttac
      481 aattacattg aaggaaagtt aggattgcat attgccagtg ctacaaaaat cttacgagct
      541 tcttctagtt cagaaaatga gcaacattac ttgcagctcc ttccaacgga accggtattt
      601 gaagtagaac aagtggctta tttggataac ggaactccgt ttgagtactc gattagtcgt
      661 catcgctatg atttatttga atttaattct tttgcattac gacattcctc ctaggagaaa
      721 atgtgaaaat gaagccaatc ttttacagac tctagtttaa gaaaaattta aaacagggca
      781 agaaggtccc atctatgctt aaatggtttc tcttttctaa ataagatggc tttaaaagag
      841 tgatcgtttt atccatcatg ttgaaaaata tcttcgtata gcttatagag taggtactga
      901 aattgttcac ctgatctact tcttatagtt atttagtttt aaatagtgtt tcaaatattc
      961 ttacactgac gagaagtttt tgagtctttt cttgtaacac atatagtata ctgaggttag
     1021 aatagtagac tgtgacttct aacaaattgc tagaaatgaa tttcaatctc ccaatttatt
     1081 tgttcaaata tt
//
LOCUS       DAVMEI010000249         1088 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.253, whole genome shotgun sequence.
ACCESSION   DAVMEI010000249 DAVMEI010000000
VERSION     DAVMEI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.253"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..977
                     /locus_tag="VYQ24_001979"
     CDS             <1..977
                     /locus_tag="VYQ24_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1969064.1"
                     /translation="SACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATI
                     AWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFE
                     AISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGMEK
                     EGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYL
                     TDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLGK
                     INAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            967..>1088
                     /locus_tag="VYQ24_001980"
     CDS             967..>1088
                     /locus_tag="VYQ24_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1969065.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLA"
BASE COUNT          427 a          155 c          183 g          323 t
ORIGIN      
        1 ttagcgcttg ttcttctaat tctgttaaaa atgaagaaaa tacttctaaa gagcatgcgc
       61 ctgataaaat agttttagat catgctttcg gtcaaactat attagataaa aaacctgaaa
      121 gagttgcaac tattgcttgg ggaaatcatg atgtagcatt agctttagga atagttcctg
      181 ttggattttc aaaagcaaat tacggtgtaa gtgctgataa aggagtttta ccatggacag
      241 aagaaaaaat caaagaacta aatggtaaag ctaacctatt tgacgatttg gatggactta
      301 actttgaagc aatatcaaat tctaaaccag atgttatctt agcaggttat tctggtataa
      361 ctaaagaaga ttatgacact ctatcaaaaa ttgctcctgt agcagcatac aaatctaaac
      421 cttggcaaac tttatggaga gatatgatta aaattgattc aaaagcctta ggtatggaaa
      481 aagaaggtga tgagttaatc aaaaatactg aagctcgtat atccaaagaa ttagaaaaac
      541 atccagaaat caaaggaaaa atcaaaggaa aaaaagtatt atttactatg attaatgctg
      601 cagatacatc aaaattctgg atttatacta gcaaagatcc aagagcaaat tatttaacag
      661 atttaggtct agttttccct gaatcattaa aagaatttga gagtgaagat agttttgcaa
      721 aggaaatttc tgcagaagaa gcaaataaga taaatgatgc tgatgtaatc ataacttatg
      781 gtgatgataa aactcttgaa gctttacaaa aagatcctct tttaggtaaa ataaatgcaa
      841 ttaaaaatgg tgccgttgct gtaattccag ataatacacc gttagcagcc tcatgcactc
      901 caacaccact ttcaataaac tatactattg aagaatacct aaatctttta ggaaatgcat
      961 gcaaaaatgc gaaataaaaa acaaataaac ctaggcataa tttttgtaat ctgcctaggt
     1021 cttcttatta caatattttt gtcattaaag cttggaacaa aagaaattaa tatcagagat
     1081 tttttagc
//
LOCUS       DAVMEI010000250         1038 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.254, whole genome shotgun sequence.
ACCESSION   DAVMEI010000250 DAVMEI010000000
VERSION     DAVMEI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.254"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            26..757
                     /locus_tag="VYQ24_001981"
     CDS             26..757
                     /locus_tag="VYQ24_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969066.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHASYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            772..>1038
                     /locus_tag="VYQ24_001982"
     CDS             772..>1038
                     /locus_tag="VYQ24_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1969067.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTID"
BASE COUNT          390 a          138 c          202 g          308 t
ORIGIN      
        1 aaaagtattt ggaggaggaa gaattatgtt gaatcaggat ctctttgatt cgcttgaggc
       61 acaaaaaatt gtagatactt tgatgaaagg gcaaaaagat tatgtagatg aacgtctaga
      121 aaaaagagag acaatgatag tgtcgaatgg ttatgcatgg acgcgaccta atcatattga
      181 tactgcattt gcatcagcag atttgtttga gtataaatta caattagcag gacagacttg
      241 gggatattta gaatttgaaa caaatacaga aaaatatggg aaagtattgt taattataaa
      301 gggtaagaag cgacttacga accaatttcc tttggtacaa aaaaataaga gtggctactt
      361 atttgaatat gctcagatga atacacttta tcttaatcaa catgcttcct acaaaaatga
      421 tgaagatagt cattcctttc caattcagat ggagttagtt tctgatgaaa tgattcaaga
      481 aattgaacaa gctactaaaa attcgaatat cgaaaaattt atgattttaa cttatgaggc
      541 ggactcagaa aacaatatta tatctgtaga tgttgttatg cctgatgcac gaactggtca
      601 gttacacttg attcaagatt tgtctgagta tattcaatca agttcgtacc atttcgaaga
      661 agctaaatat caagatattc ctaatttttc agaattatcg gaaacagaag attttgaaat
      721 tattccaaga atagaaaaac aagaaggtca aaagtagtaa ggagtatgta aatgtttaat
      781 ggtcgggtat tgaaagaatt acggctgtta aatggtttaa gtagagcaga attagctcag
      841 agaattaatt taacggaaca agccatttgg cagtttgagt ccaacgaaac gaaacctaaa
      901 ttatcaacca aaatgcattt ggccaaccaa tttcatgttg atttaactta ttttgaacag
      961 gaagaagaga gcattcgatt tgattcttct gtaattgcct ttagaaatgc agacctagca
     1021 acacggaaaa caatagat
//
LOCUS       DAVMEI010000251         1031 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.255, whole genome shotgun sequence.
ACCESSION   DAVMEI010000251 DAVMEI010000000
VERSION     DAVMEI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.255"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            90..278
                     /locus_tag="VYQ24_001983"
     CDS             90..278
                     /locus_tag="VYQ24_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1969068.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            340..>1031
                     /locus_tag="VYQ24_001984"
     CDS             340..>1031
                     /locus_tag="VYQ24_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1969069.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARW"
BASE COUNT          352 a          128 c          220 g          331 t
ORIGIN      
        1 aaatcctctc cacttttaaa ctaatgtatt ataaaagcca gttgacacaa gtgttacacc
       61 tgtgatagaa ttaacttgag gtgatgaaaa tggcaaccga taaaaataga ataatgatta
      121 gcttggatga taaaaatcta gagaaacttg aaaatttagt agaagatgct agagatagaa
      181 ggggaatgcg actaacaaag tcccaggtta ttgaattact cctaaatacc gttgattatt
      241 tcgatgatat tatgggggct atttactcaa aaaaataaga gcaaaattgc tattattttt
      301 tacaaattgg tgtaacacta gtgatatagg agtgatgaaa tggttgttta tattagacaa
      361 agtaaattac caagtgaggt ttcaattaat aaatacaatg ctcaagtagg tgcctactta
      421 caaggagaag aagctgtttt atatcaatct ttttctgaaa taaaagagtt aacaagtgaa
      481 gatatagttg tagattatat aatggagact agagcattac taaaaatgat gggcttaaac
      541 gttccggttc atgactatcc tattgagctt aaagagtttt atggtcgaaa gatttatgct
      601 ggtattttag gagagattgt gaatatacct gataattggg gaaaatttat taagcctaaa
      661 gctggttcaa aagtctttac tggaagagtt gttaatggaa cccatgattt aataggtatt
      721 ggtctacctt tcgactatcc tatatggatt agtgaggttg tagaattcat agctgaatgg
      781 cgttgttttg tgttagatgg tcgcgtatta gatgttcgac cctatacagg tgattatcat
      841 gcacaatttg atgcaagtgt aattgatgaa gcgatatcat gttggaaaga tgcgccaata
      901 gcttatggac tagatatcgg tgttactcgc gatggcagaa cacttgttgt tgaagtaaat
      961 gatggttatg cattgggaaa ttatggccta tctcctttaa aatcaatcaa ttttcataga
     1021 gctaggtgga a
//
LOCUS       DAVMEI010000252         1030 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.256, whole genome shotgun sequence.
ACCESSION   DAVMEI010000252 DAVMEI010000000
VERSION     DAVMEI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.256"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            3..482
                     /gene="rlmH"
                     /locus_tag="VYQ24_001985"
     CDS             3..482
                     /gene="rlmH"
                     /locus_tag="VYQ24_001985"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1969070.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            526..599
                     /locus_tag="VYQ24_001986"
     tRNA            526..599
                     /locus_tag="VYQ24_001986"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:560..562,aa:Arg,seq:tct)
     gene            764..889
                     /gene="comC"
                     /locus_tag="VYQ24_001987"
     CDS             764..889
                     /gene="comC"
                     /locus_tag="VYQ24_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1969071.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIKGGEMRISRIILDFLFLRKK"
BASE COUNT          360 a          130 c          201 g          339 t
ORIGIN      
        1 tcatgaaaat taaagttgta acagttggga aactgaaaga aaagtattta aaagatggta
       61 tcgcagagta ttcaaaacga atttctagat ttgctaagtt tgaaatgatt gagttatcag
      121 atgaaaaaac accagataag gccagtgaat cagaaaatca aaagatttta gaaatagaag
      181 gtcagagaat tttatcaaaa attgctgacc gtgatttcgt tattgtgtta gccattgaag
      241 ggaaaacttt cttctcagaa gaatttagta agcagttaga agaaacttct ataaaaggat
      301 tttctactct tacttttatt attgggggaa gtttaggatt gtcatcatct gtaaaaaata
      361 gagccaatct ttctgtcagt tttggtcgcc taaccttgcc tcatcagtta atgagactag
      421 ttcttgttga acaaatctat cgcgctttta cgattcagca gggattcccc taccataaat
      481 agaaaattga cttttaattg aatttttggt agaataattg tgttaggtct catagctcag
      541 ctggatagag cattcgcctt ctaagcgaac ggtcgcaggt tcgaatcctg ctgggatcaa
      601 tataatagca aagctgggaa ttttcccggc ttttttctta aaaaagtaca ctttgggaga
      661 aaaaaatgac agttgagaga attttatcta aagtgaaatt ccattttgta taatggtttt
      721 tgtaagttag cttacaagaa aaaacatttt aggagatttt attatgaaaa acacagttaa
      781 attggaacag tttgtagctt tgaaggaaaa agacttacaa aatattaaag gtggggagat
      841 gaggatttca agaatcatcc ttgattttct tttcctacga aaaaagtaat gaaataagga
      901 gaaagagtaa tggatttact tggatttggg acagttattg ttcatttttt aattattagt
      961 cacagttacc gtttaatttg taaaggtcga ataaatagaa aagaattata catttttggt
     1021 gcttatacat
//
LOCUS       DAVMEI010000253         1015 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.257, whole genome shotgun sequence.
ACCESSION   DAVMEI010000253 DAVMEI010000000
VERSION     DAVMEI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.257"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            92..274
                     /locus_tag="VYQ24_001988"
     CDS             92..274
                     /locus_tag="VYQ24_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1969072.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGHFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            284..634
                     /locus_tag="VYQ24_001989"
     CDS             284..634
                     /locus_tag="VYQ24_001989"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HEV1969073.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYGDSAWEDLRAVTGID
                     LLSILTDIANKKQG"
     gene            642..>1015
                     /locus_tag="VYQ24_001990"
     CDS             642..>1015
                     /locus_tag="VYQ24_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV1969074.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNN"
BASE COUNT          419 a          125 c          171 g          300 t
ORIGIN      
        1 gaaatgaccg aattagaaag aaaaaatcga aaaattagct aagaaatatt ctgataactt
       61 aaacatcaaa gttcaagaga gagttcgtga aatggcaaat gataataaga gccattattt
      121 gatatacaga gttttaggta tttcatttga agaaggagaa aatatcgatt tgtatcaaaa
      181 taaaggtcat tttttataca aatatgctgg ttcattttta gaagaagctg cagtactatc
      241 ctttaacgaa aaatttggta cagaaaatac ttaaaaagtt aacattccta attctgaaag
      301 tacaaaacct aagacttttg aaattgattg tttagtcgga gaaaaacacg catacgaaat
      361 aaaatggtgg gatgcaacta cagatggaga ccatataact aaagaacaca ctagaataaa
      421 agttattcat aacaaaggat atataccaat tcggttaatg ttctactatc caaatagaac
      481 tcaagctata aaaattcagc aaactttaga aacattgtat aacggtattg gagggaaata
      541 ttatggagat tctgcctggg aagatttaag agcagtgacc ggtattgatt tacttagtat
      601 tctaacagat attgcaaata aaaaacaggg gtaaaatcaa aatgacagta ttaaaaggag
      661 ataacttaga aatattaaaa actattgaat cctcaagtat tgatttaatc tatatggacc
      721 ctcctttctt tacacagaaa acccaaaaat tatctaataa caaaaatatt atgtattcat
      781 tcgaagatac gtggacttcg attgaggatt acaaagaatt tttgtctgta agattagaag
      841 aatgcaaaag agtgctaaaa aatagtggca gtattttcgt tcattgtgat aaaattgcaa
      901 atcatcatat tagattaatt ttagataata tctttggagc agatatgttt caaagcgaaa
      961 ttatatggaa ctataaacgg tggtctaatt caaaaaaggg attattgaac aatca
//
LOCUS       DAVMEI010000254          988 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.258, whole genome shotgun sequence.
ACCESSION   DAVMEI010000254 DAVMEI010000000
VERSION     DAVMEI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.258"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>988
                     /locus_tag="VYQ24_001991"
     CDS             <1..>988
                     /locus_tag="VYQ24_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1969075.1"
                     /translation="KTQQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDG
                     QESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTAT
                     LAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQ
                     IGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLN
                     NVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYS
                     NKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEKKATFVSKNTIKTEDFN"
BASE COUNT          374 a          125 c          204 g          285 t
ORIGIN      
        1 aaaaactcag caaagtgaaa ctggggtata tacatcattt aaaaatttag tagatgcaat
       61 gaatagcaat cctaatggca cattccgttt aggagcaact atggatgctc gtgaagttga
      121 acttccagat ggtcaagaaa gttatgtgaa taatgtattc catggaatat tagtaggaac
      181 aaataatgag aaatattatg ctatttataa tttgaaaaaa ccattatttg gggaattaaa
      241 tggtgctaca gtagaaaaac tttcactaaa agatgttaat atttccgcaa aggacgatac
      301 tgcaacactt gcgaaagaag caaataacaa tactcatatt gataacgttc atgctgatgg
      361 agcgatagct ggggaacgta gcataggagg attagtatca caagttaata attctactat
      421 ttctaacagt agctatacag gtagaataac taatacatat aaaacagttg ctagttatca
      481 aatcggaggt ttagtaggca aactttctgg tccgagggga ttaatagata agtcgttcgc
      541 atcaatcgat ttatcttcaa atgcaacaca aggagaccag tctattggtg gaatagttgg
      601 tgcagtagaa aatagtgcgc ttattagtaa tagttatgct gaaggaaatc taaataatgt
      661 tcaacgtttt gcaaacgttg gtggagtagt tggtaatctt tgggatccgg ttggaggatt
      721 agaaaaatcc ggtcgacttt ctaatgtact aagcgatgtt aatgtaacga atggtaatgc
      781 tattgcaggt tataatttta atggtataaa agcaaatggc acatacagta ataaaaataa
      841 taaggttgtg aacgtagtac aagaagatga tgaaatctta actaaggatt ctactgttca
      901 aagaggagaa gtattagaag atgcacaaat aaaagagaaa aaagctactt ttgtatctaa
      961 aaatactatt aaaacagaag attttaat
//
LOCUS       DAVMEI010000255          976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.259, whole genome shotgun sequence.
ACCESSION   DAVMEI010000255 DAVMEI010000000
VERSION     DAVMEI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.259"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..976)
                     /locus_tag="VYQ24_001992"
                     /pseudo
     CDS             complement(1..976)
                     /locus_tag="VYQ24_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
BASE COUNT          324 a          176 c          108 g          368 t
ORIGIN      
        1 tctcttatcc agatccccct tgtgtaaact ttatctttat aagcttcaag gcccctatcc
       61 catctatttg caacaattag atcactttgt tttgtaaata gttcaaaatt cttttcaaaa
      121 attacgttat ctatactaac gtttaaattt ggttcatata ctaaaatttt tataccgaca
      181 atcaatagtt cattaattat acttaaaata gctgactctt tgtaattatc tgaattatat
      241 ttcatcccca atttatatat tcctactatc tttggctttc gttccaatat ttgtttaact
      301 atgaactgtt ttctatttgt gtttgaaata tcaatcgctt ctatcactgg ggcatttatt
      361 tctataaatt ctttttttaa ttgtttagta tctttgggaa gacaatatcc tccaaatcca
      421 aaagaaggat tattataaaa atttccaatt cttggatcta aacaaacacc ttttattaca
      481 acttcagcat ttaaacttct cctctcagca aaaaaatcta gttcattaaa aaaagcaaca
      541 cggagagcta agaatgtgtt agaaaaaagc ttaattgctt ctgcttcagt aggagaaact
      601 aacataacat ttttaatatt ggcaatacta tgagtactaa tcgaaaggaa caactctgca
      661 atttttcttc cttcaactgt ctcatctcca acaactatgc gacttggata taaattatca
      721 tatatagaac aaccttctct caaaaattca gggacaaaaa tgatattttt tgtatcaaac
      781 agccttttta atttgtttga aaagccgatc ggaactgttg actttcaaat aatctttcca
      841 ttaggtttta ccctcagaat cttcgatacc gtttgttcga tttcatatgt attaaaacta
      901 ccaattttct catcataatc tgtcggaagc gcaataatat aataatcaat attattttta
      961 atttcagaaa atgtat
//
LOCUS       DAVMEI010000256          975 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.260, whole genome shotgun sequence.
ACCESSION   DAVMEI010000256 DAVMEI010000000
VERSION     DAVMEI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.260"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..304)
                     /locus_tag="VYQ24_001993"
     CDS             complement(<1..304)
                     /locus_tag="VYQ24_001993"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV1969076.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAV"
     gene            complement(294..>975)
                     /locus_tag="VYQ24_001994"
     CDS             complement(294..>975)
                     /locus_tag="VYQ24_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV1969077.1"
                     /translation="DFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVREVESIWNEY
                     ETFNNIRVLDVGADLAYFKHLKLLSNELDEVLSQVIVEMIDFYNIITVKRGLSQNKSH
                     GDILQLLSDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSE
                     LEFLCDLLLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISALQNTIPFESIKE
                     RIRPHYGS"
BASE COUNT          344 a          168 c          126 g          337 t
ORIGIN      
        1 ctactgcttt ctctacatta tcctctatac gttttaatcc taaatttaaa ccttgtttat
       61 gagtcggtaa taaaataata gcaggcacaa cttgggaatc ataatggcga attgtatcta
      121 atatcattga agcaatgtct tcagtgatat aaatgacacc ataatcagat tgagctaatt
      181 ttcttagtgt atttatagct tcttgttctt ggtaggcagg aaatatatca aacccaatca
      241 tgctaaatgg taaaataata tcacggctac caattatgcc aattttatac ttattagctt
      301 ccataatgtg ggcgtatcct ttcttttatt gattcaaagg gaattgtatt ttgaagagct
      361 gatatgatca ttctgagatt ctttacttca aactcacatc ccaataaata tctagcaaga
      421 actaacggcc cctctacatt gtaccttcct tgatctaaag ttttatacaa tagtaaatca
      481 cataaaaatt ctaactcaga agatgaaatt gttgcgtcct gcatcttcaa ttcataagtt
      541 gaaaagattg aatctaagct tggatttatt ttattgaacc acacaaatat ttcttgattt
      601 tctacaatgt atataaattc tttagcagaa atacttcctt catctgaaag taattgtaaa
      661 atatccccat gactcttatt ttgagataaa ccacgtttta cagtaataat attataaaag
      721 tcaatcattt cgacaataac ctgagacagt acctcatcta actcattaga taaaagtttc
      781 agatgtttaa aatatgctag atcagctccg acatcaagta cacgaatatt attaaaagtt
      841 tcatactcat tccaaattga ttctacttca cgaaccataa aatcaggaag tgtatctgaa
      901 tgtaaggaag aaactaaatg ttttaaactt tctatatcaa aaatccctat tggaattaat
      961 aatttagaaa aatct
//
LOCUS       DAVMEI010000257          957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.261, whole genome shotgun sequence.
ACCESSION   DAVMEI010000257 DAVMEI010000000
VERSION     DAVMEI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.261"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>957
                     /locus_tag="VYQ24_001995"
     CDS             <1..>957
                     /locus_tag="VYQ24_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1969078.1"
                     /translation="DAFRFGTDEKEDKDTYQATFETNHPAIKHFFGPAGNNVVHNSNG
                     AYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRNGLGPEFYAKGLLQ
                     APDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGSVDDLNKYMHNMFDVIYMLEI
                     LEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVYATNVVRRLKPEELTKLTTFNSLI
                     EHDIITRRGYVDEATYKRNGYYTINLFSPIYSALSSKIGTPGDLMGRRIAFELLAAKG
                     YKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVTDEIVLSKV"
BASE COUNT          367 a          136 c          178 g          276 t
ORIGIN      
        1 tgatgcattt agatttggta cagatgaaaa agaagataaa gatacatatc aagcaacttt
       61 tgaaactaat catccagcta taaaacattt cttcggtcca gcgggaaata atgttgttca
      121 taattctaat ggagcatatg ctacagggga tgcattctat tatatggcat atcgcatgct
      181 tgataaagat ggtgcggtaa cttatacaca cgaaatgaca cataactcag atagagaaat
      241 ttatcttgga ggctatggaa gacgaaatgg acttggacca gagttttatg ctaaaggact
      301 gctacaagct cctgatcatc ctaatgatcc aacagtcact attaattcca tattaaaata
      361 tgatcaatca gaagaatcaa caaggcttca agtagcagat ccaactcaac gatttggaag
      421 tgttgatgat ctaaataagt atatgcataa tatgtttgat gtaatataca tgcttgaaat
      481 actagaaggt aaagctgtag ctaaattaga tacaaatcaa aaatatgatt tattaagaaa
      541 aatagaaaat gaatataaac ctgatcctga tggaaattct gtttatgcaa cgaatgttgt
      601 acgtagatta aaaccagaag aacttactaa attaactaca tttaacagtt tgatagaaca
      661 tgatattata acaagaagag gttatgtaga tgaagcaact tataagcgga atggctacta
      721 tacaattaat ctattctcac caatttattc agcattgagc agtaaaatag gaacaccagg
      781 agatttaatg ggacgacgta ttgcatttga gttattggca gccaaggggt acaaagacgg
      841 tatggttcca tatatttcta atcagtatga aaaagaagcg aaagctcaag gaaaagttat
      901 tacatcatat ggtaaacaaa taggtttagt tactgatgaa attgttcttt caaaagt
//
LOCUS       DAVMEI010000258          954 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.262, whole genome shotgun sequence.
ACCESSION   DAVMEI010000258 DAVMEI010000000
VERSION     DAVMEI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.262"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(427..825)
                     /locus_tag="VYQ24_001996"
     CDS             complement(427..825)
                     /locus_tag="VYQ24_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1969079.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          293 a          187 c          137 g          337 t
ORIGIN      
        1 tttgtggttg aacaacaagt ataacacaga ggtgttttct tatgcctaca agagctttca
       61 ttatttccat tttcttttgg atttcactct attctgaaaa acttgtgtat atttactgaa
      121 gctagcaagt cttacctgta aatttaatga aagcaacaca aaatccgaga ggggaatctc
      181 ggattaatag atagagagtt tttagtttaa ataaattgtt taaaatatca acaacatcac
      241 ttcttttctt aacctgataa gtcttgattc ctaattttgg ggctacgatt atattgtcct
      301 caatatcgtc tagaaagaca caatttctag gttataactg gtatttatcg atagttactc
      361 atatacatca gtccacctcc atacttatgt gcgagcctct ctttgtatta tacctccata
      421 ctcaccttac agattctttt ggtaataata tctttgccta atgtagagat agtcttgcaa
      481 agaaaaaact tccttgtagc catgtttctg ataaaagtcc ggtgcctgga actggtaagt
      541 attgacaaag gcaaaacaac aatttcgatt cttagcttca ctttctgcct gttgcaatag
      601 ttttgaaccg attccttgcc ctcgcagttc ctcttttaca aacaaatact cgatttctag
      661 ccaatttcca aaagtctctg ctatcaaacc tgccaggaga ttgccctttt catcttcgac
      721 ataaagatta agtggctcac tttcagcctc ttctcttttt gaacggttat aaacacgaat
      781 cagattccct atttcttgcg atttatgtga ttccttattt tccaatctaa agtatagtga
      841 aatgaaataa aacatgcgca aatcgattaa ggaatttaat ctaatttcta acaatgtctt
      901 agaaatcaaa gtgtactatt ttaacttcaa tgcactatac atctaatact caat
//
LOCUS       DAVMEI010000259          949 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.263, whole genome shotgun sequence.
ACCESSION   DAVMEI010000259 DAVMEI010000000
VERSION     DAVMEI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.263"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            35..832
                     /locus_tag="VYQ24_001997"
                     /pseudo
     CDS             35..832
                     /locus_tag="VYQ24_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          314 a          195 c          152 g          288 t
ORIGIN      
        1 tatggttctg attttctagt ataaaaaaat tcaaactaga attcttctta gaaaaggccg
       61 ttttaagtgc tatcattgct caaaaatgga gctcgctgag acttctatcg tcaagaagaa
      121 tcatcaaatt ccttgtatca tcaaccaaaa gattgctcaa aagttaattg aaaagatttc
      181 tatgaccgac attgatcatc agttgtctat ttcaacttca actgtcattc gcaagatcaa
      241 taattttcac tttgagcatg atttttcgcg tcttcctgag attatgtcct aggacgttga
      301 aacagtccgg ggagtgactg tttcaatcgg gagatggaga tgagctttat tgctcaggac
      361 tttgataaac tcaatatcat aactgttctt gagagcagaa cacaagccat catccgaaat
      421 cccatgaata caaggctatc aagcgctact ggaagctcat tcaacaagat agtcagaaac
      481 tgagtgataa acgattttat cgccctactt ttcgtatgca cttgactaat aaagaaattc
      541 tagataaact tttgagctat tccgaagact tgaaacacca ctataatctc tatcagctct
      601 tgcttttcca cctctgtttc agactttgtt taaaaccttc ctcaaagata aagagaaaat
      661 cgtcaacgcc cttcaattac cttattccaa cgccaaactg gaagccacca ataatctcat
      721 caaacttatc aagcacaatg cctttggttt taggaacttt gaaaacttca aaaaagaaag
      781 gacgaaattt gtcctttcta gatcttcgct ttcttcaacc cactacagtt gacaaagaac
      841 cgaaaaatgt ctcgcctcta atatagtcaa ttgaaacaag aacaagagaa aagagcctcg
      901 taaaaggtat tgcaacttgg taataccttt ttgaggttct ttttgatat
//
LOCUS       DAVMEI010000260          932 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.264, whole genome shotgun sequence.
ACCESSION   DAVMEI010000260 DAVMEI010000000
VERSION     DAVMEI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.264"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..249
                     /locus_tag="VYQ24_001998"
     CDS             <1..249
                     /locus_tag="VYQ24_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969080.1"
                     /translation="LLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLP
                     IFQYMVNSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            259..>932
                     /locus_tag="VYQ24_001999"
     CDS             259..>932
                     /locus_tag="VYQ24_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969081.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTR"
BASE COUNT          247 a          109 c          178 g          398 t
ORIGIN      
        1 ttacttacta ttagcagttt ttcttggttt aaaaaatcaa acatgagctt agtatttctg
       61 tttacttttt tatttgtaga atccttattc tggatttatc agttggacaa tgggataatt
      121 ggattattgc caatttttca gtatatggta aattccaatc cgtatgcatt gatttattgg
      181 cttacattac tatctatcat aattccattg actgtatttt ctgttcatag aaactggagg
      241 agagtgtaaa agttggaaat gggaaagtta agtagtcaca tgtggaggtt gaatcagata
      301 atctatacca agtacttttg gggttatgtt cttttttgga tattgatttg tttaggatta
      361 tggtattggt tagaaggaaa tgatagactt gttatagaaa ttttaaaagg gcctaatctg
      421 agtcaaaact cttttttagt cttatctata tggttgcttc attggtttat tattcataca
      481 ttttttctag cagttgtata tcgtagaaga gcatccgatt tctttatgga agtgattcga
      541 ttttcttcta ttaagctctg gattaggtat cagatttgga cctgttttct ttatggactc
      601 attttaatca tggtaaaagt tctagtgatt caatttatgt tacagttacc aaactgggat
      661 ataggagttt tgtttatagt tgattctttg aatgcttgtg tgttagtctt gttttgcttt
      721 atgttatacg cactaggagc gaatgtacaa atgaactttg cttgcgttag tttcttttta
      781 ctcatgattg tgtttggtgg tctatttgta ggaaatcgaa ctaattatct attttatatt
      841 ttgaatcgag ggaatggaga tataggacga gatttatttc ttcagttgct atttttagtc
      901 tttcttttta aaagtatttt ctatttcact cg
//
LOCUS       DAVMEI010000261          929 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.265, whole genome shotgun sequence.
ACCESSION   DAVMEI010000261 DAVMEI010000000
VERSION     DAVMEI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.265"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(153..>929)
                     /locus_tag="VYQ24_002000"
     CDS             complement(153..>929)
                     /locus_tag="VYQ24_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1969082.1"
                     /translation="IDSFPLIWFHTLLDKVLRTCKEFGVNAIVEYFGEEDTISNSIIS
                     STGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSGVVYVSNNNFQATYDMMK
                     AVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDLKVIYGLSTIDSVYSYAM
                     QHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISIIGFDNIPISQYYTPALST
                     IAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT          338 a          187 c          121 g          283 t
ORIGIN      
        1 cgaatgtgac catgtcttcc ttagatcgaa catgaacatg tcccacttgt tagaaattgg
       61 atccaactca atagaaactg aatggaggct aaacagaact tattttagaa cacttcatct
      121 tttccactag gattttcaag aattaaacaa tactagaaac tctgtctcct aacaaattta
      181 ggagaaactt caacagatgt gacactttcc cctttaataa ttgctaaaac accttctatc
      241 atttctttag ccaatttaac ataattggga gcaattgtag acaaagctgg agtataatac
      301 tgagaaatag gaatattatc aaatccaata atagaaatat catctggaat aagaattcct
      361 ttctcatagc acgcacgaat caagccctga accttttcat ctcctgaaac aaaaataatg
      421 tccggataat tttgggtagt caagtgctgc attgcataag aataaactga atcaattgta
      481 gataagccat aaatgacttt taaatccata aagtaatttt tatcattcag aaaagaacgc
      541 acacctcttt cacgatcctt attaacatgg gattctcccc ccataagcaa ccacatattt
      601 ttaaattttt cttcagttac agctttcatc atatcataag tagcttgaaa attattatta
      661 gatacataga ctactccaga cgtttgagat tcaccgaaaa caagaaaagg catatggttc
      721 ttctttaaat actgaattct gatatcatct acactttcat aaaaaacaat aacaccatct
      781 actaggctac ctgtgcttga tataattgaa ttactaattg tatcctcctc tccaaagtac
      841 tcaactatag cattaacacc aaattcttta cacgtccgta acactttatc taacagcgta
      901 tgaaaccaaa ttaaaggaaa agagtcgat
//
LOCUS       DAVMEI010000262          911 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.266, whole genome shotgun sequence.
ACCESSION   DAVMEI010000262 DAVMEI010000000
VERSION     DAVMEI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.266"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            207..476
                     /gene="blpJ"
                     /locus_tag="VYQ24_002001"
     CDS             207..476
                     /gene="blpJ"
                     /locus_tag="VYQ24_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV1969083.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            545..793
                     /gene="blpK"
                     /locus_tag="VYQ24_002002"
     CDS             545..793
                     /gene="blpK"
                     /locus_tag="VYQ24_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEV1969084.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
BASE COUNT          300 a          108 c          214 g          289 t
ORIGIN      
        1 attgcaagga gaataacgaa attaagatat tttagatact caagattatt gaaatcttag
       61 actaaaaaat aggtactgct acctttaaga agatagtaga cgtatatact tttttaagaa
      121 aatcaaaaag atactaaaaa aaatctattt gtttattgaa tttaagactt tggtaacaaa
      181 ttgaaaataa aaaggaggta ttcatcatga atacaaaaat gttgtcacag ttagaggtta
      241 tggatactga aatgcttgcg aaagttgaag gggggtatag ctccactgat tgtcaaaatg
      301 cactgattac aggagtcact acgggaatta taactggtgg aactggagca ggtttggcca
      361 ctttaggagt agctggactt gctggtgcat ttgttggagc acacattggt gcaattgggg
      421 gtggattaac atgcttagga ggtatggttg gtgataaatt gggactaagt tggtaagact
      481 tatagtaaaa ttatgaggat tctattctga tttattttag ttttttaaaa aaggagtatt
      541 tgtcatggat acaaaaatga tgtcacaatt ttctgttatg gatactgaaa tgcttgcttg
      601 cgttgaaggt ggcggatgca attggggaga ttttgccaaa gcaggtgttg gaggagcagc
      661 tgtagttgct gctttaggtt gtgcagcggg tggagtgaaa tatgggaaaa ttctaggacc
      721 atggggcgct gcaataggag gaattggagg agcagtggtt tgtggatatt tagcctatac
      781 cgctacatca taaaaatgaa agctaaattt tatgaaggct atattcttta ttattctctt
      841 cgcttttcaa acctatctta tttatctatc cataagtatc tctgataaaa aacaaaagac
      901 tattgaatta a
//
LOCUS       DAVMEI010000263          907 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.267, whole genome shotgun sequence.
ACCESSION   DAVMEI010000263 DAVMEI010000000
VERSION     DAVMEI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.267"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(55..>907)
                     /locus_tag="VYQ24_002003"
     CDS             complement(55..>907)
                     /locus_tag="VYQ24_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1969085.1"
                     /translation="LIDYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKL
                     GISKDNHDLLYKLERSGIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDL
                     AHELGHILLHKYKDMNEDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDNY
                     ILLKSDLNVSIQALEYRAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSK
                     VKSILDVVLSNHLVSLATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIP
                     LYKRNNL"
BASE COUNT          307 a          172 c          113 g          315 t
ORIGIN      
        1 tgataaaatc caatttctgc catatatcct tcatgaaatt atttcacagt taaattataa
       61 attatttctt ttgtacaaag ggatgatgtt atcaaatcga tctgttcttc tatcttcttg
      121 atactgatca aaaaatttca tttcgactga aaatatttcg cttataaact gtaaacgaat
      181 actttgttta gacattatag tcgctagact gactagatga ttactcaaaa cgacgtccag
      241 aatactcttt actttgcttg gttttttaac aaaaatttga tcatccaaag gttcaatcat
      301 tttgtaacct ttttgcgcaa tttgacgata aaagtaagaa tgttgctttg gagtcaataa
      361 tcctaactta aaagctcgat actctaaagc ctgtatcgaa acattcaaat ccgacttcaa
      421 taaaatataa ttatcaggat tgctgacacg cttgccaacc ctctcttcaa atttgactaa
      481 aaactcttct tttggcaata aaaaacatga tgcaaaataa tttgcttctt gctccaaacg
      541 atcgccatct tcattcatat ctttatattt atgtaaaaga atatgtccta gctcatgagc
      601 taagtcaaaa tttcgacgta cagatgattt attcgttccc aacacaatat aaggtcttcc
      661 caattttgac catgcgctat aagcatcagc ttggccatta attaatcgtt ccacgatata
      721 gatgcctgaa cgttctaatt tataaagcaa atcatgatta tcttttgaaa tacctaattt
      781 ttccctggca taaagagcca attcctcaat ggattctccc ttatgataag attcactcac
      841 tacattactt aggtcatgaa ttgtaatatt aggtataatt acaaaacttt caaaataatc
      901 aatcaaa
//
LOCUS       DAVMEI010000264          902 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.268, whole genome shotgun sequence.
ACCESSION   DAVMEI010000264 DAVMEI010000000
VERSION     DAVMEI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.268"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            333..689
                     /locus_tag="VYQ24_002004"
     CDS             333..689
                     /locus_tag="VYQ24_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969086.1"
                     /translation="MKRNIRSVRKQFRLTETEEKQILDLMREKGEDNFSDFLRKSLLL
                     SDGQKQMEKWFNLWKKQKLEQICRDVHEVLIISKSDHQVTQEHVSILLTCIQELIKEV
                     ENTSPLSEDFRNKYMR"
     gene            694..>902
                     /locus_tag="VYQ24_002005"
     CDS             694..>902
                     /locus_tag="VYQ24_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEV1969087.1"
                     /translation="MEHRYRTNLKKVFLSDLELVKLNENISKSNCLSFSEYARRTLLN
                     PGMNFITIDTNSYQDLIFELKRIGN"
BASE COUNT          322 a          129 c          164 g          287 t
ORIGIN      
        1 tgagagtcaa gaattattga ccctcttttt tatcatctca tttccctgtg ataaaatttc
       61 caactttcct ttgctatact tttctcaact tgtaaaaatc tatgttagat tttctatggg
      121 ggtttggggg aggagccacc aagttatcat atcgttagct ttcaaaacta gaaggttttg
      181 atcagctggc gatatgattt ttaggatatt gtggacacaa tatctgagct cgcaaagcct
      241 tacaaaatgt tgaagctatt ttgaaaaatg tactgacagt gtctgtaagc ttacattgtc
      301 agtacaggta tcaatgaagg aggaaaagtt atatgaaaag aaatatacgt agtgttcgga
      361 aacaatttcg cttaacagaa acggaggaaa aacaaatact agatttaatg agagagaaag
      421 gagaggataa tttttctgat tttctccgta aaagcttact attgtccgat ggacaaaaac
      481 agatggaaaa atggttcaac ctttggaaaa aacaaaagtt ggaacaaatt tgtcgcgatg
      541 ttcatgaagt tttaatcatt tctaaaagcg atcatcaggt tactcaagaa catgtttcta
      601 ttttgttaac ttgtattcag gaattaatta aagaggtaga aaataccagt cctcttagtg
      661 aagattttcg taacaaatac atgaggtagt agagtggagc accgatatag aacaaattta
      721 aaaaaagtct ttttatctga cctagaatta gtcaaattga atgaaaatat ctcaaaaagt
      781 aactgcttat cattctcaga atatgctaga cgaactctac taaatcctgg tatgaatttt
      841 atcaccattg atacaaatag ttatcaagat ttgatttttg aattaaaacg aatcggaaat
      901 aa
//
LOCUS       DAVMEI010000265          895 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.269, whole genome shotgun sequence.
ACCESSION   DAVMEI010000265 DAVMEI010000000
VERSION     DAVMEI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.269"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            147..>895
                     /locus_tag="VYQ24_002006"
     CDS             147..>895
                     /locus_tag="VYQ24_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1969088.1"
                     /translation="MSNNVEIIKANPLVTRRNDNSGSSSRRVAAYCRVSSDSDDQKNS
                     YDSQVRHYREYISQHQDWNLVDIYADEGISGTQVGKRQDFQRLISDCLDGKIDYIITK
                     AIARFARNTLDTLKYVRLLKDKQIGVFFEEENIDTLTMDGELLLTILSSVAQQEVENT
                     SAHVKKGLKMKMQRGELIGFQGCLGYDYNQDTKSISINEKEAKIVRYIFERYIDGIGG
                     KAIARELDELGYKTPRGLDHWQDTTVLGIIK"
BASE COUNT          340 a          109 c          177 g          269 t
ORIGIN      
        1 atgctaaatc tattattcct acaaaaactt attagcgaaa aagaatatga aaaaataaag
       61 ctgaaactgc gtaaaatata cacaaattag ctgacctttt ttattggttg cggtaaaata
      121 aatgcagaga aataggaggt taagccatgt caaataatgt tgaaattata aaagcaaatc
      181 ctttagtaac tagaagaaat gataatagtg gttcttcatc gaggagagta gcagcatatt
      241 gtcgtgttag ttccgatagt gatgatcaaa aaaatagcta tgattctcaa gttcgtcatt
      301 atagagagta tatatcacag caccaagatt ggaatttggt tgacatttat gctgatgaag
      361 ggatttcagg aactcaagtt ggaaaaagac aagattttca aagattgatt tcagattgcc
      421 ttgatggaaa aatagactat atcattacta aagctattgc tcgatttgct agaaatacat
      481 tagatactct caaatatgtt cgtttgctta aagataaaca aattggagta ttttttgaag
      541 aagaaaacat tgatacttta actatggatg gtgagttgct attaacgata ttgagttcgg
      601 tggctcaaca ggaagttgaa aatacatctg cccatgttaa aaaaggttta aaaatgaaga
      661 tgcaacgtgg agaacttatt ggttttcaag gttgtcttgg ttatgactat aatcaagata
      721 caaagtcaat atcaataaat gaaaaagaag ccaagatagt acgttatatt tttgaacgat
      781 acattgatgg tattgggggt aaggcaatag ctagagaact agatgaacta ggttataaaa
      841 ctccgagagg tcttgatcac tggcaagata caacagtttt aggaattatt aaaaa
//
LOCUS       DAVMEI010000266          894 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.270, whole genome shotgun sequence.
ACCESSION   DAVMEI010000266 DAVMEI010000000
VERSION     DAVMEI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.270"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..321
                     /locus_tag="VYQ24_002007"
     CDS             <1..321
                     /locus_tag="VYQ24_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1969089.1"
                     /translation="KSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNG
                     KMAKSEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSG
                     KWVK"
     gene            408..>894
                     /locus_tag="VYQ24_002008"
     CDS             408..>894
                     /locus_tag="VYQ24_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1969090.1"
                     /translation="MSTIDKSGVLPFSAQTVQADIQTLTPMAKYYIILPKDAEIYKTW
                     RGTVNIPIIDATKTTPELSYFKEDHRNYIANENKSGANYIEWKGTVEEFKEAIKKLTD
                     KKSTTATPKKDEKPTPKPDEKPKPTPTVQSGWVGSSYYQDGKKVISKWIFDKKYNSYF
                     YL"
BASE COUNT          338 a          139 c          173 g          244 t
ORIGIN      
        1 aaatcaggcg gatacatggc taaaagtgag tggatttatg ataagaacta tggttcatac
       61 tactatctaa catcagaagg cagctacgct cgtaacactt ggtcaggcaa ttattatttg
      121 aagtcaaatg gtaagatggc taaaagtgag tgggtatacg atagtaacta caaatcatat
      181 tactacttga catcagaagg aagttatgct cgtaacactt gggtaggcaa ttactatctt
      241 aaatcaaacg gtaaaatggc tgttaatgaa cgtacaccag atggttatcg tgtagatggt
      301 tcaggtaaat gggttaaata agaaactaaa aaataggata ataggtaata tcagatgaat
      361 aaaaaaaatt attttcatgt gctttagcat tttcaacgat tggaacattg tcaacgattg
      421 ataaatcagg agttcttcct ttttcagcgc aaacggtgca ggctgacata caaacgctta
      481 ccccaatggc aaaatattat attattcttc cgaaggatgc agaaatatat aaaacttgga
      541 gaggtactgt caatattccc attattgatg ctacaaaaac tactccagaa ttatcttatt
      601 tcaaagaaga tcatcgcaac tatatagcta atgagaataa gtctggtgcc aattacatag
      661 agtggaaggg aacagtagaa gagtttaaag aggccattaa gaagttgact gataaaaaat
      721 caactactgc aacaccaaaa aaggatgaaa agccaacacc aaaaccggat gagaagccaa
      781 aaccaacacc tacagttcag tctggttggg taggttcatc ctattatcaa gatggtaaaa
      841 aagtcattag taagtggatt tttgataaaa aatataattc atacttctat ctag
//
LOCUS       DAVMEI010000267          892 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.271, whole genome shotgun sequence.
ACCESSION   DAVMEI010000267 DAVMEI010000000
VERSION     DAVMEI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.271"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..66
                     /locus_tag="VYQ24_002009"
     CDS             <1..66
                     /locus_tag="VYQ24_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1969091.1"
                     /translation="YSGYLDGIERMLEISEKRMVA"
     gene            66..833
                     /locus_tag="VYQ24_002010"
     CDS             66..833
                     /locus_tag="VYQ24_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1969092.1"
                     /translation="MGIQDYTDSEFKQALERNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFARKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEVQLALIATKPKLSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEKLAIFERIQIYQRDRSCVYDSKENTT
                     SAAEVLQECLFGKWSKVEEEMLKVGEEILETLCNRTE"
BASE COUNT          337 a          135 c          189 g          231 t
ORIGIN      
        1 tatagcggct atttggatgg aattgaacgt atgttagaga tatctgaaaa acggatggta
       61 gcctaatggg aatccaagat tataccgata gtgaattcaa acaggcttta gagcgaaacc
      121 ttcgctctct gacaagagga aaaaagtcca gtaagcaacc tatagcgatt ttgcttggag
      181 ggcaaagtgg tgctggaaag actacaattc atcgtattaa acagaaagag tttcaaggaa
      241 atattgttat catagatggc gatagttttc gttctcagca tccacactat ttagaacttc
      301 agcaagaata tggcaaagat agtgttgaat acaccaagga ttttgcaagg aaaatggtag
      361 aatctttagt aacagaattg agtcatttgg gatacaatct tttgatagag ggaactttac
      421 gaacgattga tgttccaaag aaaacagcac aactcttgaa aagtagggga tatgaagtac
      481 aattagcctt gattgcgaca aagcctaagc tgtcctatct gagcaccctt atccgatacg
      541 aagaactata cgctatcaat ccaaatcaag cacgtgcaac tccaaaagaa catcatgatt
      601 tcattgtaaa tcatctagtt gataatacac gacaattaga aaaactagct atctttgaaa
      661 gaattcaaat ttaccaacga gatagaagtt gtgtatatga ttcaaaagaa aatacaactt
      721 cagcagcaga agtgctacaa gaatgcctct tcggaaaatg gagcaaggta gaggaagaga
      781 tgttgaaggt gggagaagag attttagaaa ccttgtgtaa tcgtacagaa taatgatgaa
      841 gaaaaaatat taggaagtga aatatagctg attatgatag acaatttaaa aa
//
LOCUS       DAVMEI010000268          885 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.272, whole genome shotgun sequence.
ACCESSION   DAVMEI010000268 DAVMEI010000000
VERSION     DAVMEI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.272"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(295..540)
                     /locus_tag="VYQ24_002011"
     CDS             complement(295..540)
                     /locus_tag="VYQ24_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969093.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
BASE COUNT          352 a          171 c           97 g          265 t
ORIGIN      
        1 gaagtatatt aaatataaaa gtaaaaccgc catcaatata cttacaaaaa tccccttgga
       61 ctcaatcaat ggaaaataaa atattgcaac tagaagcgaa taaagtcccg ctaataaata
      121 tttctgatca tctttcattc aaaacaccat attatttact aagaatacat caatacaata
      181 gagcaactct gtaagtcaat atttgataaa gcaaagcttt aaccccttcc ccagacgtta
      241 gcttttcttc aacccactac agttgacaag gagcccaaat tattgataaa aactttagtc
      301 atcatcgtct ctataaaaat attttctata cactcttgat aataccaaaa ttaatgccaa
      361 tatcatacca agagctataa taatagaatt tattagattt attttagtat atccaatcac
      421 tagtccaata attggactag cagacatgct tagatataat aaaaataaca aaaaatgaat
      481 ttcaccaatt cctgcaaaaa aatgtttcat agctatagtt ttacgcccat aaatacccat
      541 caaatttacc gtctacgata tctaaaccat atgcaatggc tcctcctaat gagaagactt
      601 cagcaacagt agccgcaata ttaagagcat tagtaatagt tgccatacta actccaccaa
      661 tatatcttgt tatagcactt agttacctag ataagatatc ctttagcttg cttccaaaat
      721 ttaagtctac acctataata tgagttcata ttagtactgc taaatcctat ctatagtaat
      781 gtcaaacacc taaataaagt catctctcca attcattcaa gaaatgaaga aaggcaaaaa
      841 atctttaaaa taagaaaagt gcatattatc aggtgttcaa aaata
//
LOCUS       DAVMEI010000269          881 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.273, whole genome shotgun sequence.
ACCESSION   DAVMEI010000269 DAVMEI010000000
VERSION     DAVMEI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.273"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(8..81)
                     /locus_tag="VYQ24_002012"
     tRNA            complement(8..81)
                     /locus_tag="VYQ24_002012"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="VYQ24_002013"
     tRNA            complement(95..168)
                     /locus_tag="VYQ24_002013"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="VYQ24_002014"
     tRNA            complement(174..247)
                     /locus_tag="VYQ24_002014"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="VYQ24_002015"
     tRNA            complement(263..336)
                     /locus_tag="VYQ24_002015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="VYQ24_002016"
     tRNA            complement(346..431)
                     /locus_tag="VYQ24_002016"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="VYQ24_002017"
     tRNA            complement(439..510)
                     /locus_tag="VYQ24_002017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
     gene            complement(533..605)
                     /locus_tag="VYQ24_002018"
     tRNA            complement(533..605)
                     /locus_tag="VYQ24_002018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Thr,seq:tgt)
     gene            complement(624..705)
                     /locus_tag="VYQ24_002019"
     tRNA            complement(624..705)
                     /locus_tag="VYQ24_002019"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(669..671),aa:Leu,seq:tag)
     gene            complement(711..783)
                     /locus_tag="VYQ24_002020"
     tRNA            complement(711..783)
                     /locus_tag="VYQ24_002020"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(748..750),aa:Lys,seq:ttt)
     gene            complement(808..880)
                     /locus_tag="VYQ24_002021"
     tRNA            complement(808..880)
                     /locus_tag="VYQ24_002021"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(845..847),aa:Asp,seq:gtc)
BASE COUNT          232 a          257 c          199 g          193 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      601 ccggctcatt tgttatatct taatgcgggt taagggactt gaacccccac gccgttaagc
      661 gccagatcct aaatctggtg cgtctgccaa ttccgccaaa cccgcatata tgacccgtac
      721 tgggctcgaa ccagtgaccc attgattaaa agtcaattgc tctaccaact gagctaacga
      781 gtctaaaata acttgcgtta ccttaaacgg tcccgacggg aatcgaaccc gcgatcttcg
      841 ccgtgacagg gcgacgtgat aaccgctaca ctacgggacc t
//
LOCUS       DAVMEI010000270          833 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.274, whole genome shotgun sequence.
ACCESSION   DAVMEI010000270 DAVMEI010000000
VERSION     DAVMEI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.274"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            29..>833
                     /locus_tag="VYQ24_002022"
     CDS             29..>833
                     /locus_tag="VYQ24_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEV1969094.1"
                     /translation="MKSKLGITLRKVRKGKQISLCSVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLVLHNDDYTSSESFANLVQYIRKQYSSQSINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSNIIPSDEELLHLTDYLFKVEKWGYYEIILLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSLNKTNKQIVSQLAINCFILSIDKEEFSNCSYLISKIK
                     TLLDNELNFYEQTVFLYATGYFEFKRCQSTSGIEKMKQAIQVFDILGEDK"
BASE COUNT          297 a          135 c          122 g          279 t
ORIGIN      
        1 ttaatttttg ttgggaaaat gggaaattat gaaatcaaaa cttggcatca cacttagaaa
       61 agttcgtaaa ggaaaacaaa ttagtctatg ctctgtagca gatgaacatc tttcaaaatc
      121 acaaatatct cgttttgaac gtggggagtc agagatatct tgtattcgac taatcaatat
      181 tttagacaaa ttacatatta ctttggatga gttccttgtt ttacacaatg acgactatac
      241 cagttccgaa tcatttgcta atctagtaca gtacattcgt aagcaatatt catcacaaag
      301 tatcaataat atagcatgtc tactttctga tacttctgat tacactctaa attcttttga
      361 gaaaacaatg gtaaaatcta ttttacacac aatggattcg aacatcattc catcagatga
      421 agagcttctt catctgacag actatctttt caaagttgaa aaatggggct attatgaaat
      481 cattttatta ggtaactgtg tacgcacaat taactacaac tcctacttct tattaactaa
      541 agagatgtta aataactata tttactcatc tctgaataaa actaataagc agattgtctc
      601 tcagcttgct attaattgct tcattctaag cattgataag gaagaatttt ctaattgctc
      661 ctatttaatt agtaagataa agacattgtt ggacaatgaa ctaaactttt atgaacaaac
      721 agtttttctc tatgcaactg gttactttga atttaaacgt tgccaatcaa ccagtggaat
      781 tgaaaaaatg aaacaagcca tacaagtctt cgatatttta ggagaagata agt
//
LOCUS       DAVMEI010000271          819 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.275, whole genome shotgun sequence.
ACCESSION   DAVMEI010000271 DAVMEI010000000
VERSION     DAVMEI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.275"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..365
                     /locus_tag="VYQ24_002023"
     CDS             <1..365
                     /locus_tag="VYQ24_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1969095.1"
                     /translation="LIDNKTVYDNLSLITKDKKTITDVLEKVGLSSDYLHQKIYELSG
                     GQAQRVAIARMLMKPRKIILADEPTGALDGEIGKEIIRLLLNETAEDKYVIIATHDPA
                     VYNEVDVIIDMKDIGYKV"
     gene            362..>819
                     /locus_tag="VYQ24_002024"
     CDS             362..>819
                     /locus_tag="VYQ24_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969096.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELNSQKKDIKE"
BASE COUNT          319 a          107 c          164 g          229 t
ORIGIN      
        1 ctttaataga taacaaaaca gtgtatgata atctgagcct tatcactaaa gacaaaaaaa
       61 caataactga cgtacttgaa aaagtaggac tgtcaagtga ctatttacac caaaaaatat
      121 atgaattgtc tggagggcag gctcaacgtg tagctattgc cagaatgtta atgaaaccac
      181 gtaaaattat cttagcagat gaacctacag gagctttaga tggtgagatt ggaaaagaaa
      241 ttattcgttt actattgaat gaaacagctg aagataaata tgtcattata gcgacacatg
      301 atccagctgt ctataacgaa gttgatgtga taattgacat gaaagatata gggtataagg
      361 tatgaaaaag aaaatttata ttgctttgat ctttgtaact ggggtactcg ccattttctt
      421 ttttggaaaa caaatgatta caaaagaaaa tatcaataag ccaacggtag agttaacaat
      481 ctatacgctt tcttctagtg atacagaaaa atggaataaa gtgagacaag tagaaacgga
      541 agaagctata tacttcataa ctgtaaaaga agtatcatct tcagaagaag tattttcaaa
      601 catcattgca aatggagctg ctacggggtt cggagttcgt gaagaagaag tgaagaaatt
      661 caataatggt ttaggagaca ccatagagga ctctaagcat aataaactaa ttgaaataga
      721 attttttact ttttctgatg atggcgcggg atttgtggta gcaaactttg actacggtaa
      781 agaagagttg aattctcaga aaaaagatat aaaagaatt
//
LOCUS       DAVMEI010000272          799 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.276, whole genome shotgun sequence.
ACCESSION   DAVMEI010000272 DAVMEI010000000
VERSION     DAVMEI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.276"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            76..>799
                     /locus_tag="VYQ24_002025"
     CDS             76..>799
                     /locus_tag="VYQ24_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1969097.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPD"
BASE COUNT          221 a          151 c          199 g          228 t
ORIGIN      
        1 cttgatttta tcctttattt actataaaat gagaaggaaa aatgtcaaac ttttatattg
       61 caaataggag aaatcatgac aaaaacatta aaacgtcctg aggttttatc acctgcaggg
      121 actttagaga agctaaaggt agctgttcag tatggagcag atgctgtctt tatcggtggt
      181 caggcctatg gtcttcgtag ccgtgcggga aactttactt tcgaacagat ggaagaaggc
      241 gtgcagtttg cggccaagta tggtgccaag gtctatgtag cggctaatat ggttatgcac
      301 gaaggaaatg aagctggtgc tggtgagtgg ttccgtaaac tgcgtgatat cgggattgca
      361 gcagttatcg tatctgaccc agccttgatt atgattgcag cgactgaagc accaggcctt
      421 gaaatccacc tttctaccca agccagtgcc actaactatg aaacccttga gttctggaaa
      481 gagctaggct tgactcgtgt cgttttagcg cgtgaggttt caatggaaga attagctgag
      541 atccgcaaac gtacagatgt tgaaattgaa gcctttgtcc atggagctat gtgtatttca
      601 tactctggac gttgtactct ttcaaaccac atgagtatgc gtgatgccaa ccgtggtgga
      661 tgttctcagt catgccgttg gaaatacgac ctttacgata tgccatttgg gaaagaacgt
      721 aagagtttgc agggtgagat tccagaagaa ttttcaatgt cagccgttga catgtctatg
      781 attgaccaca ttccagata
//
LOCUS       DAVMEI010000273          798 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.277, whole genome shotgun sequence.
ACCESSION   DAVMEI010000273 DAVMEI010000000
VERSION     DAVMEI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.277"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..749)
                     /locus_tag="VYQ24_002026"
     CDS             complement(<1..749)
                     /locus_tag="VYQ24_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1969098.1"
                     /translation="MQSVTRNPIADPGILGINTGASLSVVIGLSFLGISSSISHISFA
                     IIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSMALSSFVSFLILPNNNVLDKFRF
                     WQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLNALAMGDEMAVGLGVNVNRIRSL
                     AIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISKDIRTMTISSSFIGAELLLICDI
                     IGRMLGKPGEIEVGIITAIIGGPVLIYVTMK"
BASE COUNT          288 a          158 c          123 g          229 t
ORIGIN      
        1 tttttcatag ttacataaat aagtactgga cccccgatta ttgcagtaat tatccctact
       61 tcaatttcac ctggtttacc taacatacgg ccgattatat cacatataag caagagctct
      121 gcacctataa aagatgaaga aatggtcatt gtgcgtatat ctttgcttat aaataagcca
      181 caaaagtgag gaactataag acctacgaag ccaataggtc caccaattgc agtaatactt
      241 gaacataaaa gcacacttgc aattattgca agtgatctta tcctattaac attaactcca
      301 agaccaacag ccatttcatc acccatagct aaagcgttta aatctgatga aataaatata
      361 gctatcaagt gacctaaaat tataaaaggt agtagtgtag atatagaaga taatgtagct
      421 gctccaaggc tacctatttg ccaaaatcta aatttgtcta agacgttatt attcggtaaa
      481 attaaaaaac ttacaaaact gcttaaagcc atactaacac aagttcctga taaggcaagt
      541 tttatagggg taaggcctgc ttttccgctt acagcaatcg cgtatacaaa aattgcactt
      601 actaagccac caatgattgc aaaacttata tggcttatgc ttgatgaaat tcctaaaaaa
      661 gaaagaccaa ttactacact aagacttgct cctgtgttta taccgagtat acctggatca
      721 gctattgggt ttctagtaac tgattgcatc aatacaccgc ttatggcaag actagaacct
      781 gctaaaattg caaaaata
//
LOCUS       DAVMEI010000274          784 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.278, whole genome shotgun sequence.
ACCESSION   DAVMEI010000274 DAVMEI010000000
VERSION     DAVMEI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.278"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>784
                     /gene="phtD"
                     /locus_tag="VYQ24_002027"
     CDS             <1..>784
                     /gene="phtD"
                     /locus_tag="VYQ24_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV1969099.1"
                     /translation="VAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPK
                     SDLSASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENI
                     SSLLRELYAKPLSERHVESDGLIFDPAQITSRTVRGVAVPHGNHYHFIPYEQMSELEK
                     RIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSPQPAPNPQPAPSNPIDEKLVKEA
                     VRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLS"
BASE COUNT          236 a          189 c          155 g          204 t
ORIGIN      
        1 gttgctgcag ccagagccca aggacgttat acaacggatg atgggtatat cttcaatgct
       61 tctgatatca ttgaagatac tggtgatgct tatatcgttc ctcacggcga ccattaccat
      121 tacattccta agagtgattt gtctgccagt gaattagctg ctgcccaagc ctattggaat
      181 gggaagcagg gatctcgtcc ttcttcaagt tctagtcata atgcaaatcc agctcaacca
      241 agattgtcag agaaccacaa tctgactgtc actccaactt atcatcaaaa tcaaggggaa
      301 aacatttcaa gccttttacg tgaattgtat gctaaaccct tatcagaacg ccatgtagaa
      361 tctgatggcc ttattttcga cccagcgcaa atcacaagtc gaaccgtcag aggtgtagct
      421 gtccctcatg gtaaccatta ccactttatc ccttatgaac aaatgtctga attggaaaaa
      481 cgaattgctc gtattattcc ccttcgttat cgttcaaacc attgggtacc agattcaaga
      541 ccagaacaac caagtccaca atcgactccg gaacctagtc caagtccgca acctgcacca
      601 aatcctcaac cagctccaag caatccaatt gatgagaaat tggtcaaaga agctgttcga
      661 aaagtaggcg atggttatgt ctttgaggag aatggagttt ctcgttatat cccagccaag
      721 gatctttcag cagaaacagc agcaggcatt gatagcaaac tggccaagca ggaaagttta
      781 tctc
//
LOCUS       DAVMEI010000275          784 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.279, whole genome shotgun sequence.
ACCESSION   DAVMEI010000275 DAVMEI010000000
VERSION     DAVMEI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.279"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..285
                     /locus_tag="VYQ24_002028"
     CDS             <1..285
                     /locus_tag="VYQ24_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV1969100.1"
                     /translation="SIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLIST
                     LGDSKDAIKGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(233..778)
                     /locus_tag="VYQ24_002029"
     CDS             complement(233..778)
                     /locus_tag="VYQ24_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1969101.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          277 a          121 c          112 g          274 t
ORIGIN      
        1 tctattggaa aagttgccat attggatatt cctgccactc ataatgaagc cattatatcg
       61 atattcccct atgctaataa agaaaatatt atcagagatt acttaatgat atttttgcca
      121 cttatctcta ctttaggtga ttcaaaagat gctataaaag ggaaaacatt aaatagtacc
      181 agtatctccg aattattaat ccctatttct aatcatgaag agatgaaaag aatcatttcc
      241 aaagttgatt tactttttca aaaagtttct caactttttg agtaataagt tcctgttcct
      301 caaaaggagc taacggaatt aatagctcgc ttagtgtagt tttaggaata ttatataaag
      361 cttgacctga tagtttagtt attgctttca attgtttata aaataacgga gaggacaagt
      421 taaataatag aaattttgaa ataatctctg aactttcgaa tggtgttaat tggaaaataa
      481 atccaccagc cacaacacca tcatagtctt tatcgattct tgcaaacttt ccaatatgtt
      541 ctaaagaggt tgatacaggt gttattagct gattatgttt taaataaact tgttcagagg
      601 agatgaattg tgtatcaatg tagtaatcat tatccaacag agaaaattct aaaggcttaa
      661 tattaccacc acgtataatt ctaacacctt tattattaat gcttaaatcg cccttcttgt
      721 aagaaagacc tgtattcatt gaaaaaatat cttttatttt tataacaacc caattcatag
      781 gtat
//
LOCUS       DAVMEI010000276          780 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.280, whole genome shotgun sequence.
ACCESSION   DAVMEI010000276 DAVMEI010000000
VERSION     DAVMEI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.280"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            50..352
                     /locus_tag="VYQ24_002030"
     CDS             50..352
                     /locus_tag="VYQ24_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1969102.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            360..780
                     /locus_tag="VYQ24_002031"
                     /pseudo
     CDS             360..780
                     /locus_tag="VYQ24_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          263 a          145 c          160 g          212 t
ORIGIN      
        1 gcaaccgctt aatcttgtct attaaaaaat aaaatcggag gaaatccaaa tgattaaaat
       61 tcttgctgcc tgcggtgcag gtgttaactc aagtcaccaa attaaaagtg ctctagaaga
      121 agaactttca aaccgtggtt atgatgttca ctgtgatgca gtcatggtga aagatgtaaa
      181 cgaagacctt atgaaaggtt acgatatctt tacaccaatc gctgcaacag accttggttt
      241 tgaaccaggt atcccagtta tcgaagctgg gccaatctta ttccgtatcc cagcaatgag
      301 cgttccagta tttgacaata ttagacttcc tgcgaaacaa aacatggtat agtagttcta
      361 tgaatgatga agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc
      421 agcgcacgac ttttgaagag atgttagctg tattaaaaac agcttatcaa cttaaacacg
      481 caaaaggtgg acgaaaacct aaattaagcc tagaagacct tcttatggcc actcttcaat
      541 atgtgctaga ataccgcact tatgaagaaa ttgcggctga ttttggtatc cacgaaagca
      601 acttaatccg tcggagccaa tgggttgaag taactcttgt tcaaagtggt gttacgattt
      661 caaaaactca tcttagtgct gaggacacgg taatgattga tgcgacggaa gtaaaaatca
      721 atcgccctaa aaaaagaatt agcgaatgat tctggtaaaa agaaatttca cgctatgaag
//
LOCUS       DAVMEI010000277          774 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.281, whole genome shotgun sequence.
ACCESSION   DAVMEI010000277 DAVMEI010000000
VERSION     DAVMEI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.281"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..774
                     /locus_tag="VYQ24_002032"
                     /pseudo
     CDS             1..774
                     /locus_tag="VYQ24_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          241 a          141 c          172 g          220 t
ORIGIN      
        1 ggtggccgta accatgcata tatgacgatt gaggaaaaga aagtctttct tgcccgccat
       61 ttgaaggcta cagaggcagg agaatttgtt acaattgatg ccttatttca ggcttataaa
      121 aaggagttag gtcgttccta cacacgtgat gccttctatc aactgttgaa gcaccatggt
      181 tggcgaaata ttatgccacg tccagaacat cctaggaaag cagacgctca aaccattgtc
      241 gcgtctaaaa ataaaatctc aattcaagaa gaaaagaaag cgctttaaaa gcagtagacg
      301 ttttcgtaag gttcgcttga tgtaccaaga tgaggctggt ttcggtagaa tcagtaaact
      361 gggatcttgt tgggctccaa taggagtagg tccacatgtc catagtcact atatacgaga
      421 atttcactat tgttatggag ctgttgatgc ccatacaggc gaatcatttt tcttaatagc
      481 tggtggatgt aatactgagt ggatgaacgc ctttttagaa gagctttcac aagcttatcc
      541 agatgattat cttttactcg ttatggacaa tgctatatgg cataaatcaa gtaccttaaa
      601 gattccgact aatattggtt ttacctttat tcctccatac acaccagaga tgaacccatt
      661 gaacaagtgt ggaaagagat tcgtaaacgt ggatttaaga ataaggcctt tcgaactttg
      721 gaagatgtca tgaatcaact tcaagatgtc atacaaggac tggagaagga ggtg
//
LOCUS       DAVMEI010000278          760 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.282, whole genome shotgun sequence.
ACCESSION   DAVMEI010000278 DAVMEI010000000
VERSION     DAVMEI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.282"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            43..>760
                     /locus_tag="VYQ24_002033"
     CDS             43..>760
                     /locus_tag="VYQ24_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969103.1"
                     /translation="MLVDFINNSQKQYSFINVEGISVEDIPDDKTLKFYFYGQEKEEI
                     FNYQEEHDLFIYYLDPITGIEIPLENTIKIVETEEETDLIIKIGDYVFQDVKRHRFPD
                     GSVQLHFGESFTMSFDIKKKQFKFNYTRPDLLSKAIKCTQVFQELGKIGYFTLNGNKI
                     ELDERSIKDISSLDLEADIKGLLKISNFMKKMGIQKDVDLSCFDKQSQRNLNILYSGL
                     VLKKKVALNYNESKLLHLNIA"
BASE COUNT          291 a           94 c          128 g          247 t
ORIGIN      
        1 tccatagaga tttttaaact ggatgagaaa aaaatgtatc caatgttagt ggatttcata
       61 aacaatagtc agaagcagta tagtttcata aatgtcgaag gtatttcagt tgaggacatt
      121 cctgatgata aaactctaaa gttctacttc tatggtcagg aaaaagaaga aatttttaac
      181 tatcaggaag aacatgatct ctttatttat tatttagatc ctataactgg catagaaata
      241 cctcttgaaa acacaataaa gattgtagaa actgaagaag aaacggattt aattataaaa
      301 ataggcgatt atgtttttca agacgtaaaa agacatcgtt ttcctgatgg gagtgtacag
      361 cttcattttg gtgaaagctt tacaatgtct tttgacataa agaagaaaca attcaaattt
      421 aactatacta ggccggattt gctgtcaaaa gctataaagt gtactcaggt atttcaagag
      481 ctaggaaaaa tcggatattt tacccttaat ggtaataaaa tagagttaga tgaacggtca
      541 ataaaggata tttcttcact tgatttagag gctgatataa agggattgtt aaaaatttct
      601 aactttatga aaaaaatggg tatacaaaaa gatgttgatt tatcttgctt tgataaacaa
      661 tctcaaagaa atctaaatat tctttattca ggtcttgtct taaaaaagaa agttgcttta
      721 aattataatg aatcgaaatt acttcaccta aatattgcta
//
LOCUS       DAVMEI010000279          736 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.283, whole genome shotgun sequence.
ACCESSION   DAVMEI010000279 DAVMEI010000000
VERSION     DAVMEI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.283"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            419..487
                     /locus_tag="VYQ24_002034"
                     /pseudo
     CDS             419..487
                     /locus_tag="VYQ24_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0227 family bacteriocin-like peptide"
BASE COUNT          237 a           88 c          166 g          245 t
ORIGIN      
        1 tatatcattg attagtctag ctataatctt tccatggggc tggtcgatat aatgtaaggg
       61 agcttgagtt gttagtggag gataaaatgt taagagtaat agtagttatt caatctttag
      121 taataatact gcttgctgtt gctttattat tatctcggaa aaactttgat tcgttaaatg
      181 ttgttcttat aatgatgatc gtatcacaat tgttgttatt agtatatttg ataaaatcag
      241 ggaatcagtg attagctgtt atttgaatta agtaaactgt taagcttcaa gcagtaactg
      301 gttttaaata tttaaaataa gataaagaaa ggaagtctaa tttaggtaac taaacaaagc
      361 ctgcgatcac ttgttaggaa accatcctaa ccatgcttaa taaaaaagga gaaaataaat
      421 ggaacttgta ttaccaaata attatgttgt gattgatgaa gaagagatga tgtaccttga
      481 tgggggagct tatttaagca agcgtgcttg tcaaggaatt tgcgcagctt tagctatgag
      541 tccaggaact tttatagcat tagctggagc tgcagtttta accaaaaaac taataaacta
      601 tattaaagtt ggaggccttg gaggttggct tattggtgca gcagcaggtg tattggctgg
      661 ggcggcagga agaatagctt actgtattgg atatggtgct cttaatagag gttgtgatat
      721 tagcgggaac ccttat
//
LOCUS       DAVMEI010000280          736 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.284, whole genome shotgun sequence.
ACCESSION   DAVMEI010000280 DAVMEI010000000
VERSION     DAVMEI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.284"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..376)
                     /locus_tag="VYQ24_002035"
     CDS             complement(<1..376)
                     /locus_tag="VYQ24_002035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969104.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKD"
BASE COUNT          311 a          117 c           90 g          218 t
ORIGIN      
        1 tatccttcaa taatcggtat aaaagatact gactcatacc ttgataccaa ataaaattcg
       61 gtatttcaat aagagcatga ggtaatattc cttttattgt agctactatt ccaatcatct
      121 gaccagatat acccaattgt attcctaaag atagcaaatc aaaaagttgc aataagggcg
      181 aaatcggaaa acagaataga tatagcaaaa aatttgtaga atttgcctgt aaaactgtca
      241 atgtaaattg taattccagt ttcggaggta tagtagcaaa agtctcattt tttaatataa
      301 agcttaaaag taaacctagt aaaaaacaga ctatacaaaa taacacatag taaatcagaa
      361 aaccactttt tctcatctaa tgccccctac taaaattaca aatattatca gaaaacaata
      421 taaaaccaga aaactccatc tcaataaact aactgtacct ttaattaaaa atagagcttt
      481 cttataatag acataggcta taaaataatt gaatggaatc aataacaaat tcaacaaaat
      541 actttttata tcattactat aacttgtcac cggaatcaaa cataacaaca atgaaaattg
      601 taagaaagag attaaatgcg tcaacatcat tagttgtgca ttcttaaaaa agaaattcat
      661 tttagaatga ttaagagcag ataattgaag taaagctaga tctaccccaa ttgaggattt
      721 atgattccct attatt
//
LOCUS       DAVMEI010000281          720 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.285, whole genome shotgun sequence.
ACCESSION   DAVMEI010000281 DAVMEI010000000
VERSION     DAVMEI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.285"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            73..669
                     /locus_tag="VYQ24_002036"
                     /pseudo
     CDS             73..669
                     /locus_tag="VYQ24_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT          261 a          125 c          128 g          206 t
ORIGIN      
        1 tagaattgta ggttttaaaa acgcttacat tgttttaaaa taaacttaaa taacacaacg
       61 gaggtatcca tcatgaaaaa gaaatggatg tattatgctg cttgttcttc taatgaatct
      121 gccgatgaca gttcatctga taaaggagac ggtggttcgc tagtcgttta ttgaccaaac
      181 tcagaaggct taattggagc aactattcct gcctttgaag aaaaatatgg tatcaaaata
      241 gaactgattc aagctggtac tggagaactt ttcaaaaaac tagagtcaga aaaagaagtt
      301 cctgtagctg atgttatctt tggtggttct tatacacaat atgctaccca cggagaactc
      361 tttgaaaact atatttcaaa agaaaatgat aatgttatca aagaatatca aaacacaact
      421 ggcttctcta ctccttatac actagatggt aatgttttaa tcgtccaccc tgatttaact
      481 aaaggcatga acatcgaagg atatagcgac cttctcaaac ctgaactaaa aggaaaaatc
      541 gcaactgctg acccagcaaa ctcttctagc gcctttgctc aattaacaaa tatgctacaa
      601 gctcaaggtg gttacaaaga tgataaggct tggtcttatg taaaagatct tttcacactt
      661 attgatggta tagtaaaatg aaataagaac aggacaaatt aatcaggaca gtcaaatcga
//
LOCUS       DAVMEI010000282          719 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.286, whole genome shotgun sequence.
ACCESSION   DAVMEI010000282 DAVMEI010000000
VERSION     DAVMEI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.286"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(46..501)
                     /locus_tag="VYQ24_002037"
     CDS             complement(46..501)
                     /locus_tag="VYQ24_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969105.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSENLQP
                     EKWNETAKQLYNAIMWLIILWNAKSDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT          215 a          143 c           79 g          282 t
ORIGIN      
        1 atctcaatta ggatatttta tagaataccc aaaattcaac aataactaaa agtaagattc
       61 cattgtattt ccttgttggt ctttcatatc acgaatttta tttttcagaa tattaaagtt
      121 acaaatttca tctctaatat cgttatccgc tctgtagcca ttaatcatct cagtcagagt
      181 tttcccttta ctaccattct ttatagtagt atttactctt tttatactgc tttcaaaagg
      241 ctcatatgtt ttatacttaa ttacttcgtc tttaaaatta aataaaggtg tatccgactt
      301 agcattccat aaaataatca accacattat agcattataa agctgctttg cagtttcatt
      361 ccacttttca ggttgtaaat tttcactccc tgtattgtca tattcttgtc tattatttac
      421 atgagaatca cctacaagcg gattaaaaag aggtagccct ttgtgattaa ttagttctaa
      481 aaaatccctt gccgcaccca tgcaacactg aataacttcc ttcttgccat caattttact
      541 ctcagcgata aatacataat attcatcttc aatagttgct cctgcatcac tatgtttttg
      601 ttgatttgct aacagcttaa tatgtgcttt aggctaaact tcaaaatctc taacaattct
      661 ttgtcttgtt tcatgtcctc tacaattcat ttaagttctc ctttacaagt ttagtaact
//
LOCUS       DAVMEI010000283          718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.287, whole genome shotgun sequence.
ACCESSION   DAVMEI010000283 DAVMEI010000000
VERSION     DAVMEI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.287"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            188..718
                     /locus_tag="VYQ24_002038"
                     /pseudo
     CDS             188..718
                     /locus_tag="VYQ24_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          254 a          131 c          136 g          197 t
ORIGIN      
        1 tcttgttctt atttcatttt actatagaaa gttagctaca gactgctcaa aacactgttt
       61 ttaggttgta gatagaactg acgaagtcag taacatctat acgacaaggc gaagctgacg
      121 cggtttgaag agattttcga agagtataat actagactaa aatcaaaaag cattatacaa
      181 tagtgatatg aaatcaacta aagaagaaat ccaaaccatc aaaacactct cgtacagcta
      241 aatatcataa acgccttcaa atcgttctat ttcgtctgat gggcaaatct tataaagaga
      301 ttatagagct tttagactgt aatcaaacaa cgatttagca aaatgtaaaa aaatatgagg
      361 agttcggact cgactctctc cttcaagaaa cacgtggtgg tcgtaaccat gcatatatga
      421 cagttgagga aaagaaagtc tttcttgccc gccatttgaa ggctgcagag gcaggagaat
      481 ttgttacaat tgatgcctta tttcaggctt ataaaaagga gttaggtcgt tcctacacac
      541 gtgatgcctt ctatcaattg ttgaagcgcc atggttggcg aaatattacg ccacgtccag
      601 aacatcctaa gaaagcagac gctcaaacca ttgtcgcgtc taaaaataaa atctcaattc
      661 aagaagaaaa gaaagcgatt taaaaccagt agacgttttc gtaaggttca cttgatgt
//
LOCUS       DAVMEI010000284          717 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.288, whole genome shotgun sequence.
ACCESSION   DAVMEI010000284 DAVMEI010000000
VERSION     DAVMEI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.288"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..209
                     /locus_tag="VYQ24_002039"
     CDS             <1..209
                     /locus_tag="VYQ24_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1969106.1"
                     /translation="RKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKMFSTTY
                     RNHRKRFGLRMNLIAGIINHELGF"
     gene            258..>717
                     /locus_tag="VYQ24_002040"
     CDS             258..>717
                     /locus_tag="VYQ24_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1969107.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEV"
BASE COUNT          235 a          125 c          148 g          209 t
ORIGIN      
        1 cacgtaaatc cagcaaactc aaaccgctaa cagttgaaga taaagcctgt aaccatgcgc
       61 tatctaagga gagaagcaag gttgagaaca tctttgccaa agtaaaaacg tttaaaatgt
      121 tttcaacaac ctatcgaaat catcgtaaac gcttcggact acgaatgaat ttgattgctg
      181 gcattatcaa tcatgaacta ggattctagt tttgcaggaa gtctaataaa gaaactagca
      241 aggtcaggcc ttactttatt ggtggacatc caggatttaa ttgtcctcta cttgatgatg
      301 aagtctatga agattattat ctagagtttg agaaagaaga gacttgctct gttccacgtc
      361 ctttcccaga aacaggtatg ttggatttcc aagatagaag tccatggcta gagggacaaa
      421 aagaaataga tcttagttat gatcttttca gcaaagatgc agtgactttg gatgaattgc
      481 aatctcgaac aattgccctt cgttctctta aacatgatag gggattgaaa gtgcattttg
      541 cagaatttcc aaacctcatc atctggtcaa ctttgaacaa gggacctttc attacctttg
      601 aaccatggtc tggcttgtca acattccttg aagaaggaga tcatttagaa gataagaaga
      661 atgtttgtct cttagaagcc aatcaggttg aagaattagg gtttgagata gaagttt
//
LOCUS       DAVMEI010000285          711 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.289, whole genome shotgun sequence.
ACCESSION   DAVMEI010000285 DAVMEI010000000
VERSION     DAVMEI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.289"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            27..569
                     /locus_tag="VYQ24_002041"
     CDS             27..569
                     /locus_tag="VYQ24_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1969108.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDVIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(610..683)
                     /locus_tag="VYQ24_002042"
     tRNA            complement(610..683)
                     /locus_tag="VYQ24_002042"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Asn,seq:gtt)
BASE COUNT          259 a          133 c          119 g          200 t
ORIGIN      
        1 gtctaatttg tttacaagga ggaaatatgc aagaaagtaa caaacgctta aaaacaaagc
       61 gaactattga aaatgctatg gtacaattac tgatggaaca gccatttgat aaaatttcta
      121 ctgtcaaatt agtagaaaaa gccggaatta gtcgttccag cttctatact cactataagg
      181 ataagtatga tgtgattgag cactatcaaa gcaagctatt ccatacattt gaatatattt
      241 ttcaaaaaca tgctcatcac aaaagagacg ctattttaga agtctttgaa tatctagagt
      301 cagaaccact tctggctgcc cttctttctg aaaatgggac taaagaaatc caaaatttct
      361 tacgaaataa acttcatatc atgctcagta tagatttaca aaagcgattt atgcaactga
      421 atctaaatac cactgaatta gaatatagta gcatctatct aactcatgca ctttttggtg
      481 tctgccaaac ttggattgca catggaaaaa aagaaagttc tcaagaaata acagacttcc
      541 ttatgaaaat gcttggtgat acaaattgat acaaaaagag gaacacagct gtgttccctt
      601 ttatctatac tccgccagta ggactcgaac ctacgacatc atgattaaca gtcatgcgct
      661 actaccaact gagctatggc ggataaaata gtccgtacgg gattcgaacc c
//
LOCUS       DAVMEI010000286          697 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.292, whole genome shotgun sequence.
ACCESSION   DAVMEI010000286 DAVMEI010000000
VERSION     DAVMEI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.292"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(56..>697)
                     /locus_tag="VYQ24_002043"
     rRNA            complement(56..>697)
                     /locus_tag="VYQ24_002043"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          157 a          207 c          165 g          168 t
ORIGIN      
        1 ccccacaaga cctctcaaga ctaaacaaga ccaatgcgca gttccttatc cttagaaagg
       61 aggtgatcca gccgcacctt ccgatacggc taccttgtta cgacttcacc ccaatcatct
      121 atcccacctt aggcggctgg ctccttacgg ttacctcacc gacttcgggt gttacaaact
      181 ctcgtggtgt gacgggcggt gtgtacaagg cccgggaacg tattcaccgc ggcgtgctga
      241 tccgcgatta ctagcgattc cgacttcatg taggcgagtt gcagcctaca atccgaactg
      301 agactggctt taagagatta gcttgccgtc accggcttgc gactcgttgt accagccatt
      361 gtagcacgtg tgtagcccag gtcataaggg gcatgatgat ttgacgtcat ccccaccttc
      421 ctccggttta ttaccggcag tctcgctaga gtgcccaact gaatgatggc aactaacaat
      481 aggggttgcg ctcgttgcgg gacttaaccc aacatctcac gacacgagct gacgacaacc
      541 atgcaccacc tgtcacctct gtcccgaagg aaagctctat ctctagagcg gtcagaggga
      601 tgtcaagacc tggtaaggtt cttcgcgttg cttcgaatta aaccacatgc tccaccgctt
      661 gtgcgggccc ccgtcaattc ctttgagttt caacctt
//
LOCUS       DAVMEI010000287          696 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.293, whole genome shotgun sequence.
ACCESSION   DAVMEI010000287 DAVMEI010000000
VERSION     DAVMEI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.293"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>696)
                     /locus_tag="VYQ24_002044"
     CDS             complement(<1..>696)
                     /locus_tag="VYQ24_002044"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969109.1"
                     /translation="NKKIVESWIPKLFITAFNIKQKQDFNLKFELVSNCFFQDQKNFN
                     ISPFYPDSESRSIEETKQMISASFGSKVINYNDNNEDFLDQAFENFNMRHIPHESLEE
                     KVVRNIPISLGFVNNQLSQDMLDHIISEFFKSCYNPEDASVQLISGERLNKLKGIIKK
                     QLENSVVNAYHTSSEKIFTDYLTKLDKEYSHPKMKSDFIKEFRIFLDRFDVEIKKTLS
                     SSDLTMMGIINRY"
BASE COUNT          225 a          110 c           85 g          276 t
ORIGIN      
        1 aaatacctat tgataatacc catcattgtt aaatcgctag atgacaaagt cttttttatc
       61 tcaacatcaa acctatccaa aaatattcta aactccttaa taaaatctga cttcatttta
      121 ggatgagaat attctttgtc caattttgtt aagtaatctg tgaaaatttt ctcagaactt
      181 gtatgatatg catttactac tgagttttct agttgctttt ttataattcc tttgagtttg
      241 tttaaccttt caccactaat taattgtacc gacgcatctt cagggttgta acatgattta
      301 aaaaactcgg aaataatgtg atcaagcata tcttgcgata gttgattgtt cacaaaacct
      361 aaacttattg gtatattcct cactaccttt tcttctaatg attcatgagg tatatgtctc
      421 atattaaagt tttcaaaagc ttggtccaga aaatcctcat tattatcatt gtaattaata
      481 actttcgaac caaaagaagc actgatcatt tgttttgtct cttcaatact tctactttcc
      541 gaatctggat aaaatggtga aatattaaaa tttttttgat cttgaaaaaa acagttagaa
      601 actaattcaa attttaaatt gaagtcttgt ttttgtttta tattgaacgc agttataaat
      661 aatttgggaa tccagctttc tactattttt ttattt
//
LOCUS       DAVMEI010000288          692 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.294, whole genome shotgun sequence.
ACCESSION   DAVMEI010000288 DAVMEI010000000
VERSION     DAVMEI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.294"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            89..385
                     /locus_tag="VYQ24_002045"
     CDS             89..385
                     /locus_tag="VYQ24_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HEV1969110.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
BASE COUNT          233 a           92 c          114 g          253 t
ORIGIN      
        1 gacgaacatt tgaagagagt taggaataca acatgtgcga attaactctc aataaaacat
       61 acataatata caaaaggagt acactaaaat gaaatctaaa agaatggaat ttcataataa
      121 atttcttatt gtttcagcaa tgttagcagt cattagttgg ttaagcttag gtgttgtttc
      181 ttttcctatg ttggctggga cattaggaat ttcgacaaaa gctgctgcta ctgtagtcaa
      241 tttgattagt gcttatagta cagttacagc agtaatttca attgtaggtg caatcacagg
      301 agtaggttct attggttcgg gtattgcagc aacagttctt tatatattga aaaagaaagg
      361 tgctgctaaa gcagctctat ggtagaacta gatgaaaata ctttctaaaa aagaatgtga
      421 aagtatttta ggttatctta attttattgg gataaccctt ttgttttttg ttatcataag
      481 tttgtcaatc tattttaaaa gctatcttcc ttttcaaggg aaattatttt tacttttttt
      541 ggtagtagat tacgctactt tgaatcgtta taatatgaaa ctgcctatcc aaagtaaaaa
      601 aataaatact ttctatataa ggaaaaacta tatttttctc tatcttcttg ccattgtcaa
      661 gaaatattat tctttccaaa ttttacaatc at
//
LOCUS       DAVMEI010000289          691 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.295, whole genome shotgun sequence.
ACCESSION   DAVMEI010000289 DAVMEI010000000
VERSION     DAVMEI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.295"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..574
                     /locus_tag="VYQ24_002046"
     CDS             <1..574
                     /locus_tag="VYQ24_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1969111.1"
                     /translation="LAKHIAILPQSPIIPESITVADLVSRGRFPYRKPFKSLGKDDLE
                     IINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSILLLDEPTTYLDISYQI
                     ELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKLVAEGKPEDILNDKLVK
                     DIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT          253 a          128 c          113 g          197 t
ORIGIN      
        1 tttagcaaaa cacatagcta tattacctca atctccaata atccctgaat caataacagt
       61 agctgatctt gtaagccgtg gtcgtttccc ctacagaaag ccttttaaga gtcttggaaa
      121 agatgacctt gaaataataa acagatcaat ggttaaggcc aatgttgaag atctagcaaa
      181 taacctagtt gaagaacttt ctgggggtca aaggcaaaga gtatggatag ctctagccct
      241 agcccaagat acaagtatcc tacttttaga tgagccaact acttacttgg atatctcata
      301 tcaaatagaa ctattagacc tcttgactga tctaaaccaa aaatataaga caaccatttg
      361 catgattttg cacgatataa atctaacagc aagatacgct gattacctat ttgcaattaa
      421 agaaggtaaa cttgttgcag agggaaagcc tgaagatata ctaaatgata aactagttaa
      481 agatatcttt aatcttgaag caaaaattat acgtgaccct atttccaatt cgcctctaat
      541 gattcctatt ggcaagcacc atgttagctc ttaataattg aatattggaa ttcatttaaa
      601 agtcgcaaat tttgaactga ccctcaaaag ttaggccaaa aaactaacta aaggaggtca
      661 gttttttaat ggcaaataca gcacagaatt t
//
LOCUS       DAVMEI010000290          684 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.296, whole genome shotgun sequence.
ACCESSION   DAVMEI010000290 DAVMEI010000000
VERSION     DAVMEI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.296"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..684)
                     /locus_tag="VYQ24_002047"
                     /pseudo
     CDS             complement(1..684)
                     /locus_tag="VYQ24_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          214 a          130 c          142 g          198 t
ORIGIN      
        1 agattgtcct caataagtcc gaaaaatttc tctggttcct tattctgaaa gtgaaacagc
       61 aagagttgat agagctgata gtgatgtttc aagtcttgtg aatagctcaa aagcttgtct
      121 aaaatctctt tattggttaa atgcatacga aaagtagggc gataaaaatg tttatcgctg
      181 agtttacggc tatcctgttg aatgagtttc cagtagcgct tgatagcctt gtattcatgg
      241 gattttcgat gaaactgatt catgatttgg acacgcacac gactcatagc acggctaaga
      301 tgttgtacaa tatgaaagcg atcaagaacg attttagcat tcgggagtga aacagtctgg
      361 gagactgttt cagcctgagc ctagaaattt gaaagcgaag ctgtttagcc aagtcatagt
      421 aaggactaaa catatccata gtaataattt tgacgcgaca tcggacaact ctatcgtagc
      481 gaagaaagtg atttcgaatg atagcttgtg ttctaccctc aagaacagtg ataatatcga
      541 gcttttcaaa atcttgcgca atgaagctca tctccatctc ccgattgaaa cagtcactcc
      601 ccggactgtt tcaacctccc aagacataat ctcaggcaga tttctaaaat tacactcaaa
      661 gtggaagtca ttgagcttgc gaat
//
LOCUS       DAVMEI010000291          642 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.297, whole genome shotgun sequence.
ACCESSION   DAVMEI010000291 DAVMEI010000000
VERSION     DAVMEI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.297"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            105..584
                     /locus_tag="VYQ24_002048"
     CDS             105..584
                     /locus_tag="VYQ24_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1969112.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWEQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          242 a           80 c          150 g          170 t
ORIGIN      
        1 gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa atggaacaaa
       61 tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt aaagaattgt
      121 atgaagaagt ccaagggact gtgtataagt gtagaaatga atattacctt catttatggg
      181 aattgtcgga ttgggagcaa gaaggcatgc tctgcttaca tgaattgatt agtagagaag
      241 aaggactggt agacgatatt ccacgtttaa ggaaatattt caagaccaag tttcgaaatc
      301 gaattttaga ctatatccgt aaacaggaaa gtcagaagcg tagatacgat aaagaaccct
      361 atgaagaagt gggtgagatc agtcatcgta taagtgaggg gggtctctgg ctagatgatt
      421 attatctctt tcatgaaaca ctaagagatt atagaaacaa acaaagtaaa gagaaacaag
      481 aagaactaga acgcgtctta agcaatgaac gatttcgagg gcgtcaaaga gtattaagag
      541 acttacgcat tgtgtttaag gagtttacta tccgtaccca ttagtaagtc atgcaaaaaa
      601 aatgaaaaaa attagaaaaa gtagttgaca aagtttgaaa ag
//
LOCUS       DAVMEI010000292          639 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.298, whole genome shotgun sequence.
ACCESSION   DAVMEI010000292 DAVMEI010000000
VERSION     DAVMEI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.298"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>639
                     /locus_tag="VYQ24_002049"
     CDS             <1..>639
                     /locus_tag="VYQ24_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969113.1"
                     /translation="QVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYHKVKDQYKN
                     ETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQGRNRKLDKD
                     GNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADHADEKGMISYYLVDPDQDS
                     SKYQKLGEIAESKFKNLGNGKEGSLKKDTTEVEHHHQENEESIKEKSSFTID"
BASE COUNT          252 a           80 c          131 g          176 t
ORIGIN      
        1 ggcaggtttc ctatattcct gtaaaaattg ataacaccgc ccctaagatt gtttcggttg
       61 atttttcaaa tcctgaaaaa attaagttga ttacaaagga tacttatcat aaggtaaaag
      121 atcagtataa gaatgaaacg ctatttgcga gagatcaaaa agaacatcct gaaaaatttg
      181 acgagattgc gaacgaagtt tggtatgctg gcgccgctct tgttaatgaa gatggagagg
      241 ttgaaaaaaa tcttgaagta acttacgcag gtgagggtca aggaagaaat agaaaacttg
      301 ataaagacgg aaataccatt tatgaaatta aaggtgcagg agatttaaga ggaaaaatca
      361 ttgaagtcat tgcattagat ggttctagca atttcacaaa gattcataga attaaatttg
      421 ctgatcatgc tgatgaaaag gggatgattt cttattacct agtagatcct gatcaagatt
      481 catcaaaata tcaaaagctt ggcgagattg cagaatctaa atttaaaaat ttaggaaatg
      541 gaaaagaggg tagtctaaaa aaagatacaa ctgaggtaga acatcatcat caagaaaatg
      601 aagagtctat taaagaaaaa tctagtttta ctattgata
//
LOCUS       DAVMEI010000293          638 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.299, whole genome shotgun sequence.
ACCESSION   DAVMEI010000293 DAVMEI010000000
VERSION     DAVMEI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.299"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            197..415
                     /locus_tag="VYQ24_002050"
     CDS             197..415
                     /locus_tag="VYQ24_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1969114.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
BASE COUNT          210 a           79 c          119 g          230 t
ORIGIN      
        1 acctgcggct agtttcctag tttgctcttt ggttttcatt gagtataaca cattgttaga
       61 agttggttta aatttcctaa tcagtttgtt cacatttacc ttcgatatat tatatcccat
      121 agttaaggtt ggtcatacag atgattatag ccatggagcc gtaaaactta gtgtttcttt
      181 agttgacaaa gatgccatga aaaaaatatc tgtaactgta ataggatatt ttgaaataaa
      241 tatagatgaa aatatcaccg atattctata cgtaaatggt actgctattc tttatcctta
      301 tttacgttct attgtttcaa tagtttcggc aattgatagc agtgaagcaa tgttgctacc
      361 taccattaat gttttagagt tactagataa atctcaacct tttgaagaag aataatttat
      421 tagctcacta aattgagggt aaggaaaagt aaaagcagta agaaaaatgt cttgcattat
      481 acagcaactt tttgggaatg agtagatgga ttgaataaaa tttgattaag agtggatgat
      541 ttatctgtag attattattg gacagttagt cttgaagtag tctaagaatt aggttataat
      601 cagtagaagc cttgctaata atgaggaggt tagtttat
//
LOCUS       DAVMEI010000294          624 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.300, whole genome shotgun sequence.
ACCESSION   DAVMEI010000294 DAVMEI010000000
VERSION     DAVMEI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.300"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>624
                     /locus_tag="VYQ24_002051"
     CDS             <1..>624
                     /locus_tag="VYQ24_002051"
                     /inference="COORDINATES: protein motif:HMM:NF013094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV1969115.1"
                     /translation="SKPYQLLQAIQSKRVVIIGAGALGSSLALKISAIGVRDIICIDG
                     DKIELDNLTRQILYTPGDIEKNLFKVSALESRIKDFFPSSQFQGIKSYITSYDDCVNY
                     IPHNSDLIIQTADYPIGKLDDWINKVSLKYNIPVIFSHFGSVGPFLIPSETGCLRCLD
                     QFLDTKTNGLNSITKNFFQNIPNSKSPSFVTGTQLNELLILDIIKKF"
BASE COUNT          225 a           93 c          104 g          202 t
ORIGIN      
        1 ttctaagcca tatcaattac tacaagccat tcaaagcaag agggtagtaa ttattggtgc
       61 aggagcatta ggtagctcac tagcgttgaa aatttcagca ataggtgtaa gagatattat
      121 ctgtattgat ggagacaaaa ttgaactaga taatttgaca cggcaaattt tgtatacacc
      181 aggtgatatt gaaaaaaact tatttaaagt ttcagcttta gaatcaagaa taaaggactt
      241 ttttcccagt agccaatttc aaggaattaa atcttacatc acaagttatg acgattgtgt
      301 gaattatata ccccacaata gcgatttgat tattcaaaca gctgactacc ctatagggaa
      361 actagatgac tggataaata aagtatcact aaagtataat attccggtaa tattttccca
      421 ttttggtagt gtaggcccat ttctgattcc atctgaaact ggttgtctga gatgtttaga
      481 tcaatttttg gatactaaaa ctaacgggtt aaattcaatt acaaagaatt tttttcagaa
      541 tatccctaac tcaaaaagtc catcatttgt aacaggtaca caattgaatg aattattaat
      601 tttggacata attaaaaaat tttg
//
LOCUS       DAVMEI010000295          620 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.301, whole genome shotgun sequence.
ACCESSION   DAVMEI010000295 DAVMEI010000000
VERSION     DAVMEI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.301"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(282..452)
                     /locus_tag="VYQ24_002052"
     CDS             complement(282..452)
                     /locus_tag="VYQ24_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969116.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(465..>620)
                     /locus_tag="VYQ24_002053"
     CDS             complement(465..>620)
                     /locus_tag="VYQ24_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEV1969117.1"
                     /translation="CTQIIVAHRLSTIKDADVIFVMKGGKIVESGNHKYLMDLGGEYY
                     SLYTKRK"
BASE COUNT          214 a          122 c           77 g          207 t
ORIGIN      
        1 caatgaaaat caaagagcaa actaggaagc gagccgcagg ctgtacttga gtacggtaaa
       61 gcaaagctga cgtggtttga agagattttc aaagagtatt aaacaaaaag aaaaaccgct
      121 actcctaaga atagcgattt aatcatgttt ttaagctact actgtagttg ctctattatt
      181 taagtgttat gaataacttc aatatcttta aagctttttg ttagatttat aatttcaact
      241 atagccatct ctactcctta gtttacagca aaaaattatg cttaattctt attgtttttg
      301 taaacaatca gcgaacccaa tactattcca ttcaacaagc ctaaagaaat accatattca
      361 actggaaaat ctaaaaaaat tgcaaacact attccaatca tcacccctag tgaggctgtt
      421 gttaaaatta catattcatt ttctttcttc attttcttta cacctcattt cctttttgta
      481 tataagctgt agtactctcc accaagatcc attaagtact tatgatttcc tgactcaaca
      541 atcttaccac ctttcattac aaaaataaca tccgcatcct taatcgttga caatctatga
      601 gctacaatta tttgagtaca
//
LOCUS       DAVMEI010000296          618 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.302, whole genome shotgun sequence.
ACCESSION   DAVMEI010000296 DAVMEI010000000
VERSION     DAVMEI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.302"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..520)
                     /locus_tag="VYQ24_002054"
     CDS             complement(<1..520)
                     /locus_tag="VYQ24_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1969118.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYEYLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFP"
BASE COUNT          215 a          117 c           80 g          206 t
ORIGIN      
        1 atgggaagtc ctcataatat attactttat aacgcataaa ttcaagtgcc cttccaatac
       61 tattcacaaa aacatgatca acatggtcac caagtgaaag cggacaatat acgacacatt
      121 tgtcgtctaa atgcattaac agctctttta tgatatcatt ctttaatgtg tcctcatttt
      181 ttaattcact atagatatga cggtatagaa aattgccatt tctatctttc ctatagagat
      241 attcatagta cgataagtgt ctaaaatcac attgtagacg ttcacaagct aacctgtctt
      301 ctttcttcct ttcttcaatc ggatatttcc caaggttaca caacttatga aattgcttag
      361 cagagggctg tagctgttgg ctcaaagggt aaccagaaaa tatagtaata acaagtacaa
      421 tttctccttc tgaagttaat tttgaaatat aatcaccaca ggaaaaaatt gcgtcatcta
      481 aatgtggaga taaaaagata tacttagtat tgttactcat aaccattccc tctacaattt
      541 atctaaaaac tcactaagtg tctgattaaa ttccacatca tcaaaaaaat tcaccttatt
      601 cttaataatg aatatttc
//
LOCUS       DAVMEI010000297          602 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.303, whole genome shotgun sequence.
ACCESSION   DAVMEI010000297 DAVMEI010000000
VERSION     DAVMEI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.303"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            279..>602
                     /locus_tag="VYQ24_002055"
     CDS             279..>602
                     /locus_tag="VYQ24_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEV1969119.1"
                     /translation="MMYLDGGFSILRWPVATAINIAFNGVLGGGAISLVRNYIRNYGL
                     RRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGDAITTIWANNDVNRRDP
                     NLNALW"
BASE COUNT          204 a           80 c          134 g          184 t
ORIGIN      
        1 aaaaaaaata aattttgaaa tagtattagt gagttcgcaa tcatttattg ctagtggttt
       61 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
      121 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
      181 acttgttagg aaacgatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
      241 tattaccaaa taattatgtt gctcttgagc aagaagagat gatgtatctt gatgggggat
      301 tttctattct gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
      361 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
      421 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
      481 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
      541 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
      601 gg
//
LOCUS       DAVMEI010000298          582 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.305, whole genome shotgun sequence.
ACCESSION   DAVMEI010000298 DAVMEI010000000
VERSION     DAVMEI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.305"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>582
                     /locus_tag="VYQ24_002056"
     CDS             <1..>582
                     /locus_tag="VYQ24_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1969120.1"
                     /translation="KDVTIFLGDNGNGKSSVFDAIQWCLTGNVERFKNVSSADIMKSV
                     LINKNSDECAVEIIFTTNLSLKRVAPRYGNIRVSCNYGNEIVRGDKNVKEYIEGLFKN
                     SKGEKFDIQEFLKSSLLAQDQVLDFISSDTANDRYRVLSSILGMNEITNLKENYEKVR
                     SLLDNEMEKKADFVKSLQKEIDLQESKIDYGYKY"
BASE COUNT          225 a           61 c          117 g          179 t
ORIGIN      
        1 aaagatgtta ctatcttttt aggagataat ggaaatggga aaagttcagt ttttgatgca
       61 attcaatggt gtttaacagg aaatgttgaa agattcaaga atgtcagtag tgcagacatc
      121 atgaaaagtg tactaataaa taagaactct gatgaatgtg cagttgaaat tatctttaca
      181 actaatttat ctttgaaaag ggtagcacca agatatggta atattagagt ttcttgtaat
      241 tatggcaatg aaattgtacg aggagataaa aatgtcaaag aatatatcga gggattattc
      301 aagaattcaa aaggtgaaaa atttgatatt caagaatttc tgaagtcctc tttacttgct
      361 caagatcagg tgttagattt catttcaagt gacactgcga acgatcgtta tagggtacta
      421 tcttcgatac taggaatgaa tgaaataact aacttaaaag agaattacga aaaggttagg
      481 agtttgctgg ataatgaaat ggagaaaaaa gcagattttg tgaaatctct tcaaaaggaa
      541 attgatcttc aagaatcaaa aattgactat ggatataaat at
//
LOCUS       DAVMEI010000299          580 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.306, whole genome shotgun sequence.
ACCESSION   DAVMEI010000299 DAVMEI010000000
VERSION     DAVMEI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.306"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..580)
                     /locus_tag="VYQ24_002057"
     CDS             complement(<1..580)
                     /locus_tag="VYQ24_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1969121.1"
                     /translation="MIPVYIFQQVNVLLVSLYLLKFLCIGELTILQIIYGASLISFLW
                     MYGQRKQVAKVNMETRIKWIGIGFVSLLIISLYFSLIHAQESTNQANLIGLQHQVPWF
                     SFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTILAWCLYVSLG
                     MFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLFN"
BASE COUNT          219 a          117 c           82 g          162 t
ORIGIN      
        1 tattaaaaag cagcaaacta taaactagta ggttccacac taaatgtagc cccatactgc
       61 cccacaagtc cgatttatag cgtaccatcc ctaaaaacat tcccaatgaa acatataaac
      121 accaagctag aatggttcct ggatgatgag ctaaggcaaa taaaacactt gtcaaagcaa
      181 ctcgaatatc taattttcta accaagttcc ataaaatttc tctatacaaa aattcttcaa
      241 ccatactcgc attgattaag aacaataaaa atgaaaacca aggaacttga tgttgaagcc
      301 caattaagtt tgcttgattt gtgctttctt gagcatgaat cagactaaaa tatagactga
      361 taatcagtag gctaacgaat ccaataccaa tccactttat cctagtttcc atattgacct
      421 tggccacttg ttttcgttga ccatacatcc ataaaaaaga aatgagcgac gctccataga
      481 taatctgtag tatagttaac tcaccgatac aaagaaattt cagtaagtat aaggatacca
      541 ataggacatt tacttgttgg aatatataaa ctggaattat
//
LOCUS       DAVMEI010000300          578 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.307, whole genome shotgun sequence.
ACCESSION   DAVMEI010000300 DAVMEI010000000
VERSION     DAVMEI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.307"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>578)
                     /locus_tag="VYQ24_002058"
     CDS             complement(<1..>578)
                     /locus_tag="VYQ24_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1969122.1"
                     /translation="VKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYWFYEGGS
                     FIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSLCTFDFD
                     NIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQSNQIILTKAL
                     ERVAMEILNCKMDAPAADFFFKAKANEWISIV"
BASE COUNT          196 a           99 c           73 g          210 t
ORIGIN      
        1 attactatac ttatccattc atttgccttt gctttaaaga aaaaatcagc ggcaggagcg
       61 tccatcttac aatttaatat ttccattgcc actctttcta aggcctttgt aagtattatt
      121 tgattcgatt gaagcaaggt tgaataaaaa gattctggat taatgttaat agatgctaaa
      181 tgtttttcta ttagctcttt tttaaaacca atggaaacag caagataaca acaattctcg
      241 tgtaataaaa aaacaaaatc atcttttata ttatcaaaat caaaagtaca taaagagttt
      301 gcggtaatag tttgatacgg attaaacttt tctccgtttg cactgacaat gtaacttgaa
      361 taaattgaaa catagtctga catactataa gtgctatttt gaactacttc ctctttgata
      421 taaaaatcat gtatatcgat aatgaagctg cctccttcat aaaaccaata taggccttct
      481 gcataagttg aattctcttt tttccaacaa aatgtatgac ctgcacttga atacttctca
      541 ttgtttttac attcatctac tttcatataa tcttttac
//
LOCUS       DAVMEI010000301          571 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.308, whole genome shotgun sequence.
ACCESSION   DAVMEI010000301 DAVMEI010000000
VERSION     DAVMEI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.308"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>571
                     /locus_tag="VYQ24_002059"
     CDS             <1..>571
                     /locus_tag="VYQ24_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HEV1969123.1"
                     /translation="TNSSINAFCIGGEKGINEIPEVTKIAKFYGINLLVDTVMSQDLE
                     YFPDIVWRLEGSLFECGVILQYYLGRLLFQKGNTSIFCGESADEIMTFKYHSVNYNQF
                     CNDKQKSVYFSYSDYPFYVTNSIVLKKNNLLLHSFGIHPRYPYKMSEIVEMSKKISNL
                     NDKKEFHKKNCELRFQDSVLDNINSVPGTT"
BASE COUNT          205 a           70 c          103 g          193 t
ORIGIN      
        1 tacgaattct tcaattaatg cattttgcat aggaggagaa aaaggaatta atgagattcc
       61 tgaagtaact aaaattgcaa aattttatgg tataaattta ttagttgata ctgtaatgag
      121 ccaagattta gaatattttc ccgatatagt atggagattg gaaggaagtc tctttgaatg
      181 tggtgtcata ctgcagtact atttaggcag gttgttgttc caaaaaggga acacaagtat
      241 tttttgtggc gagtcagcgg atgaaataat gacttttaag tatcattctg tgaactacaa
      301 tcaattttgt aatgataaac agaagtcagt atattttagt tattccgact atccatttta
      361 tgttacaaat tcaattgttt taaagaaaaa taacctactt ttgcatagtt ttggcattca
      421 tcctagatat ccatataaaa tgtctgagat tgttgaaatg tctaaaaaaa tatcaaatct
      481 aaacgataaa aaagaatttc acaaaaagaa ctgtgagttg aggtttcaag atagtgttct
      541 tgataacata aatagtgtcc cgggtacaac t
//
LOCUS       DAVMEI010000302          570 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.309, whole genome shotgun sequence.
ACCESSION   DAVMEI010000302 DAVMEI010000000
VERSION     DAVMEI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.309"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..355
                     /locus_tag="VYQ24_002060"
     CDS             20..355
                     /locus_tag="VYQ24_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969124.1"
                     /translation="MGGILLLIGPFVLLGTAVNTVATTLNGGATAGAFSGVALLLNAL
                     KIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGWVGGILVIIGGSLFLA
                     TLKKFKSEE"
BASE COUNT          163 a           89 c          127 g          191 t
ORIGIN      
        1 tagtggtatc gtcggtcttg tgggaggaat tttacttctt attggtcctt ttgtcttgtt
       61 gggaacagcg gtaaacacag ttgctacaac tcttaatgga ggagctactg caggggcttt
      121 ttcaggtgtt gccttactct tgaatgcctt gaagattgca aatcttgttc ttggtatcat
      181 tgctattgtt tactataaag gagataatcg tgtaggtgca gctccgtctg tactaatgat
      241 tgtttttggt ggagttagtc tcattccatt cttaggatgg gttgggggga ttcttgttat
      301 tatcggagga tctctattcc ttgcaacatt gaagaaattc aaatcagaag aataaaaggt
      361 attttagcat gaaaagaaca aaaaagttta tcggtatagg agtagctcta ttatctcttt
      421 ttcttctagt tgcatgtgga acataaagtt caaaggatac ttcaacaagt aatgatgaga
      481 agacagtagc aacatccaat agttcaaaag aaacaatcac tttcgataca ccggttgtaa
      541 cagacgatgt gattgaatca atacgcactt
//
LOCUS       DAVMEI010000303          561 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.310, whole genome shotgun sequence.
ACCESSION   DAVMEI010000303 DAVMEI010000000
VERSION     DAVMEI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.310"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..536)
                     /locus_tag="VYQ24_002061"
     CDS             complement(<1..536)
                     /locus_tag="VYQ24_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969125.1"
                     /translation="MVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQEYCQGQPY
                     GLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFVQSYLGGNTA
                     WIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAIFENLLDSY
                     LLQNQSEVDLDIFYKVLP"
BASE COUNT          204 a           87 c           85 g          185 t
ORIGIN      
        1 aaaggtaaaa ctttataaaa aatatctaag tcaacctcac tctgattctg aagcaagtag
       61 gagtcaagca aattttcaaa aatagctatc aatatgattg gtgtaaggtt tttgtattca
      121 catgtcaagt attctgagta tcgtgcagat gatatttctt ttacataact ttttgaaagc
      181 aagatttttt caaattcatc atctattatt aatcttctat ttccaatcca agcggtatta
      241 ccacccaaat aagattgtac aaatagcaat atgtaagaaa atatgaacgt aaatactgaa
      301 agaggtagaa ttctatgcgt aaaaaaatag acgataatgg tagccaggag taaagatatc
      361 tctcgtgaca atcgaattct tttcagcatc ttttttaaca gtccgtaagg ttgtccttgg
      421 caatattctt gaactaaatt aatcggaatg acatctctca ccatttctgg aaagtagagt
      481 aagtttatag gttgaaatct ccaactccca tcataagtaa atggatatag aaccaaatac
      541 ttaaaagatt tttttcttat a
//
LOCUS       DAVMEI010000304          555 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.311, whole genome shotgun sequence.
ACCESSION   DAVMEI010000304 DAVMEI010000000
VERSION     DAVMEI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.311"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>555
                     /locus_tag="VYQ24_002062"
     CDS             <1..>555
                     /locus_tag="VYQ24_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1969126.1"
                     /translation="NDTLYEFRHDRQEVNQRKIHSHHIIQSFSPDDHLTPEQINRIGY
                     ETVKELTGGRFRFIVATHVDKDHIHNHIILNSIDQNSDKKFMWDYKAEHNLRMVSDRL
                     SKIAGAKIIENRYSHRQYEVYRKTNYKYEIKQRVYFLIENSKNFEDLKKKARALNLKI
                     DFRHKHATFFMTDSTMKQVVRDNK"
BASE COUNT          210 a           88 c           89 g          168 t
ORIGIN      
        1 gtaatgatac tctttatgaa tttcgtcatg atagacaaga agtaaatcaa cgaaaaattc
       61 attctcatca catcattcag tccttttctc cagatgacca tctcactcct gaacaaatca
      121 atcgaattgg ttatgagaca gttaaagagt tgacaggagg aagatttcgt tttattgtag
      181 caactcatgt cgataaagat catatccaca atcacatcat cctaaattca attgatcaga
      241 attctgataa aaagtttatg tgggattata aggcagaaca taatctacga atggtttctg
      301 atcgtctttc aaaaattgca ggggcaaaaa ttatagaaaa tcgttattcg catcgtcagt
      361 atgaagttta tcgcaaaaca aattacaaat atgaaataaa acaacgagta tattttctaa
      421 tcgagaactc gaaaaatttt gaagatctta agaaaaaagc tagagcttta aatttaaaaa
      481 ttgattttag acacaagcat gccacctttt ttatgacgga ttcaactatg aaacaggtgg
      541 tgcgtgataa taaat
//
LOCUS       DAVMEI010000305          553 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.312, whole genome shotgun sequence.
ACCESSION   DAVMEI010000305 DAVMEI010000000
VERSION     DAVMEI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.312"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(23..544)
                     /locus_tag="VYQ24_002063"
     CDS             complement(23..544)
                     /locus_tag="VYQ24_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1969127.1"
                     /translation="MKFLCIGELTILQVLYGASLFSFLWMYGQRKQVVKVNMKTRMKC
                     LGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQVPWFSFLLFLINASMVEEFLYRE
                     ILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYVSLGMFLGLVRYKLDLWGSMGLH
                     LVWSLLVYSLLLF"
BASE COUNT          211 a          118 c           79 g          145 t
ORIGIN      
        1 gagataaatc aggtcaccta ttttaaaaaa gcagcaaact ataaactagt aggctccaca
       61 ccaaatgtag ccccatactg ccccataagt ccaatttgta gcgtacaagc cctaaaaaca
      121 tccccagcga tacatacaaa caccaagcta taatggttcc tggatgatgt gctaaggcaa
      181 ataaaacact tgtcaaagca actcgaatat ctaattttct aaccaagttc cataaaattt
      241 ctctatacag aaattcttca accatactcg cattgattaa aaacaataaa aatgaaaacc
      301 aaggaacttg atgttgaagg ccaattaaat ttgtttgatt cgtgcttcct tgagcatgaa
      361 tcaggctaaa acatagactt ataatcagta gactagctag tccaatacca aggcatttca
      421 tcctagtttt catattgacc ttgaccactt gttttcgttg accatacatc cataaaaaag
      481 aaaaaagaga cgcaccatag agaacctgta gtatagttaa ctcaccgata caaagaaatt
      541 tcaataagta tag
//
LOCUS       DAVMEI010000306          551 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.313, whole genome shotgun sequence.
ACCESSION   DAVMEI010000306 DAVMEI010000000
VERSION     DAVMEI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.313"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>551
                     /locus_tag="VYQ24_002064"
     CDS             <1..>551
                     /locus_tag="VYQ24_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1969128.1"
                     /translation="PSCPECENQLKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYH
                     CSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFH
                     FKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLKGRTQAVIRNHFLRYDRA
                     VRCRVKIITMDMFSPYYDLARQL"
BASE COUNT          172 a          118 c           86 g          175 t
ORIGIN      
        1 ccatcttgcc ctgagtgcga aaaccaattg aagaaatatg actttcaaaa accttctaaa
       61 attccttatc ttgaaacgac tggtatgcct actagaattc tccttagaaa gcgtcgattc
      121 aagtgctatc actgttcaaa aatgatggtc gctgaaactt ctatcgtcaa gaagaatcac
      181 caaatccctc gtatcatcaa ccaaaagatt gctcaaaagt taattgaaaa gatttctatg
      241 actgatattg cccatcagct ttccatctca acttcaactg ttattcgtaa gctcaatgac
      301 tttcacttta aacatgattt ttcttgtctt cctgagatta tgtcttggga tgagtatgct
      361 tttacaaaag gaaagatgag cttcattgcg caagatttta acaatctcaa tatcatcact
      421 gttcttaagg gaagaacaca agctgtcatc cgaaatcact ttcttcgcta cgatagagcc
      481 gtccgatgtc gcgtcaaaat cattactatg gatatgttta gtccttacta tgacttagct
      541 agacaacttt t
//
LOCUS       DAVMEI010000307          551 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.314, whole genome shotgun sequence.
ACCESSION   DAVMEI010000307 DAVMEI010000000
VERSION     DAVMEI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.314"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(13..>551)
                     /locus_tag="VYQ24_002065"
     CDS             complement(13..>551)
                     /locus_tag="VYQ24_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1969129.1"
                     /translation="EIDKGIYRVEGENGSGKSTLLQLLAGLETFDSGLKNCVSKDVLY
                     LDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINTFFDNKIGDNYMTASTGTKFK
                     LGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISMKESSTIIYVSHNLLNQELIS
                     KSQRILIEEGRVYEISNK"
BASE COUNT          187 a          100 c           68 g          196 t
ORIGIN      
        1 ttgtataaac tattatttgt ttgaaatttc ataaacccgt ccttcctcta ttaaaatcct
       61 ttgagatttg ctaatcaatt cttgattcaa taaattatga gatacatata taatagtaga
      121 gctttctttc atggaaatta agcgtttagc aatcatatca atacttcttg catccactgt
      181 tgataaagtc tcatcaataa aaatataatc ccaattttta gcaaaaatta aggataagcc
      241 taatttgaat tttgttccag tagatgctgt catatagtta tctccgattt tattatcaaa
      301 aaaagtatta atttgttctt tatcctcaaa tgatagatgg atttgaaaag aatcacacaa
      361 caactgcaaa ttttcttcta tagtaaatgg aacaacccca agctgattgg tatcaaggta
      421 tagtacatct ttggagacac aattctttaa accactatca aaagtctcta gcccagctaa
      481 aagttgtaga agggtgctct ttcctgaacc attctctcct tcaactctgt aaattccctt
      541 atcaatctct a
//
LOCUS       DAVMEI010000308          542 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.315, whole genome shotgun sequence.
ACCESSION   DAVMEI010000308 DAVMEI010000000
VERSION     DAVMEI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.315"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..237
                     /locus_tag="VYQ24_002066"
     CDS             <1..237
                     /locus_tag="VYQ24_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1969130.1"
                     /translation="IPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFGENKYRL
                     LNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            312..509
                     /locus_tag="VYQ24_002067"
     CDS             312..509
                     /locus_tag="VYQ24_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1969131.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSDISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
BASE COUNT          188 a           99 c           72 g          183 t
ORIGIN      
        1 atcccagtta ttcctaattt taacaatagt ttagaggatg cagaaaaatt cgctactcta
       61 tttaactcat taaatatcga ccaagttcaa ctactccctt ttcatcaatt tggtgaaaac
      121 aaatatcgtt tattaaatcg gaaatatgaa atggatggag tcaacgcact tcatcctgaa
      181 gatcttattg attatcaaaa ggtatttctg aaccaccata ttaattgtta tttctagttt
      241 atttccttga aatgctctag ctatttgcag ataacaagca tctatataag gttatcaaag
      301 aagacttccc catgactcta aatgaatata tcttacaata tcgcctgaaa caagctatag
      361 ataagatggc tgaatctccc aactcctctt taagcgatat ctctgaccaa gttggatttt
      421 cagactataa atattttgcc aaagtattta aaaagtatct ccatatttcc ccaaagaaat
      481 tgaaatcact cggaagaata gtaaaatagg ctattcttct ttttgtagag aattttactc
      541 ta
//
LOCUS       DAVMEI010000309          534 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.317, whole genome shotgun sequence.
ACCESSION   DAVMEI010000309 DAVMEI010000000
VERSION     DAVMEI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.317"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>534
                     /locus_tag="VYQ24_002068"
     CDS             <1..>534
                     /locus_tag="VYQ24_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HEV1969132.1"
                     /translation="LSDKNGTMIDIFTETPWCREGETEDEDYLDISIFEVFEPNDWLK
                     IDNCKIDSVIASYQRLVDNKLKYEGADRTILKIVIAADMAEDKTKRELLLNWAQCLSD
                     WNLKYSKNCEMAIINDLQIKSRVRKLNSKETETLTNILVNSNDNYELCFGSSVLLKSK
                     PQADLFWNKLDNETKER"
BASE COUNT          208 a           67 c          100 g          159 t
ORIGIN      
        1 tttgtcagat aaaaatggaa ctatgataga catttttact gaaactcctt ggtgccggga
       61 aggagaaact gaagatgaag attatttaga catttcaatt tttgaagtat ttgaaccaaa
      121 cgattggcta aaaattgata attgtaaaat tgattcagtt attgcctcgt atcaaagatt
      181 ggtagataat aagctgaaat atgaaggcgc tgaccgtact attttaaaaa tagttatcgc
      241 tgcagacatg gcagaagata agactaaaag agagttgtta cttaattggg cgcaatgtct
      301 ttctgactgg aacttaaaat actcaaaaaa ttgtgagatg gctataataa atgaccttca
      361 aataaaatct agagttcgta aattaaacag caaagaaaca gagacactaa ctaatatttt
      421 agtaaatagt aatgataact atgaactttg tttcggttca tcagtgctat taaaatcgaa
      481 accacaagct gatttgtttt ggaataaact ggataatgag acaaaagaaa gata
//
LOCUS       DAVMEI010000310          531 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.318, whole genome shotgun sequence.
ACCESSION   DAVMEI010000310 DAVMEI010000000
VERSION     DAVMEI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.318"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..255
                     /gene="tnpB"
                     /locus_tag="VYQ24_002069"
     CDS             <1..255
                     /gene="tnpB"
                     /locus_tag="VYQ24_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1969133.1"
                     /translation="IDSLAYVVKTHFELDPFSGQVFLFCGGRKDRFKALYWDGQGFWL
                     LYKRFENVRLTWPSTEKDVKALTPEQVDWLMKGFSITPKI"
     gene            complement(410..>531)
                     /locus_tag="VYQ24_002070"
     CDS             complement(410..>531)
                     /locus_tag="VYQ24_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1969134.1"
                     /translation="FQTLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFENR"
BASE COUNT          150 a          109 c           92 g          180 t
ORIGIN      
        1 atagactcat tagcctatgt agttaaaacc cactttgaat tggatccttt ctccggtcaa
       61 gtctttctct tttgtggtgg acgtaaagac cgctttaaag ccctttactg ggatggtcaa
      121 ggattttggc tactatataa acgctttgag aacgtcagac tgacttggcc cagtacagaa
      181 aaggatgtca aagctctcac acctgaacaa gtagactggc ttatgaaggg cttttctatc
      241 actccaaaaa tatagtagat tgaaactaga atagtacacc tctgcttcta aaacattgtt
      301 agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt tactataaat
      361 ccatcagaaa gtcgtgattt ctattgaaat gaggactttc tttttatact catctgtttt
      421 caaaaagcat tctagtccat ctccgattaa cgatggactt tatcacctcc ttctccagtc
      481 cttgtataac atcttggagt tgattcatga catcttccaa agtttgaaag g
//
LOCUS       DAVMEI010000311          522 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.319, whole genome shotgun sequence.
ACCESSION   DAVMEI010000311 DAVMEI010000000
VERSION     DAVMEI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.319"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            22..510
                     /locus_tag="VYQ24_002071"
     CDS             22..510
                     /locus_tag="VYQ24_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1969135.1"
                     /translation="MTVIIKSMETPEEIEDKSLVHWQTWREAYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
BASE COUNT          182 a           67 c          111 g          162 t
ORIGIN      
        1 gagaagactg ggagaaaata aatgacggtt attatcaaat caatggaaac tcctgaagag
       61 atagaagata aatcccttgt tcactggcaa acgtggagag aggcttatga tgaccttttg
      121 cctgcggaat ttcaggatac aatgacatta gaaagatgtc gattctttag tcaaaagtat
      181 ccagaaaata cattgattgc gatggatggc gtgaaggtgg ttggttttat cagttatggc
      241 aattttcgtg atgagactat tcaagctggt gaaattattg ctttatatgt tttaaaagac
      301 tattatggaa aaggtatcgc acaaaagtta atgaaagcag ctttgactgc tcttaatcat
      361 ttttctgaaa ttttcttatg ggtattgaaa gataacaagc gcgccattgc tttctatcaa
      421 aaaatgggtt ttacttttga tggacaagaa aaaatacttg aacttggaaa gcctataaag
      481 gaaaaacgaa tggtgttcta ttctaaataa ttttaaaaaa gt
//
LOCUS       DAVMEI010000312          516 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.320, whole genome shotgun sequence.
ACCESSION   DAVMEI010000312 DAVMEI010000000
VERSION     DAVMEI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.320"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>516
                     /locus_tag="VYQ24_002072"
     CDS             <1..>516
                     /locus_tag="VYQ24_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1969136.1"
                     /translation="YLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGR
                     GKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRL
                     GVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKTSPFVFFDEPSS
                     GMDYSNMIKIS"
BASE COUNT          199 a           63 c          102 g          152 t
ORIGIN      
        1 ttatttaaaa gaaggtggag agtatcagat aaaaaatctt agctacaaat ttactgatga
       61 tgagtgttta agcttaaaag atatttcatt caagcttggg aaaatttatg gcataatagg
      121 atccaacgga cgaggaaaat caacgctttt aagatgttta ataggtcttg agaaaaaatc
      181 aaaagaagaa atttatttta agggagagaa gctatctaaa aaagaaagac tcaaaaactc
      241 ttcacttgtt atgcaagatg taaatcatca attattcaca gatgaagtat tcaacgagct
      301 tagattagga gtaaagaatt ttgatgaaga aaaggcgaaa atcattttaa aagatttagg
      361 cctggacgaa tttattgaaa ggcatccgat gagtttatca ggagggcaaa agcaaaggct
      421 tgcaatagca tctgttatgt gcaagacttc tccatttgtc ttttttgacg aaccttcaag
      481 tggtatggat tattccaata tgataaaaat atctga
//
LOCUS       DAVMEI010000313          514 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.321, whole genome shotgun sequence.
ACCESSION   DAVMEI010000313 DAVMEI010000000
VERSION     DAVMEI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.321"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     misc_binding    72..301
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          175 a           85 c          107 g          147 t
ORIGIN      
        1 tggtcacatt cgaatgatat tttcaaaaaa atcgtaaaaa agtcttgaca aagaaaaaac
       61 aaagtattaa aataagttca acaaatcaga aaagtaacta tttttgatct tcagggagcc
      121 tgtggtggtt gtgaataggt ggttggaagt agtgaaagtg ggctgatttt gaaaatgaat
      181 ttgaaacaat gaaaattcgg tgcgcacacc ttacagtgca gcttgttgtt aaacaaggca
      241 gagatgtaaa gggatagtcc ctttataatt gaggtggcac cgcgttacca acgccctcac
      301 acggaagtat attctgtgtg tgggcttttt tctatccgtc gtttggttta tcttttatta
      361 gggccttaag tcgctttgat gaacttgagt tctatctaca gctcctttcc tcctactaaa
      421 agcaaacaaa aagctggact aatacgaaaa aagctagtga tactcaatga aaatcaaaga
      481 acaaactagg aaactatccg caggttgctc aaaa
//
LOCUS       DAVMEI010000314          513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.323, whole genome shotgun sequence.
ACCESSION   DAVMEI010000314 DAVMEI010000000
VERSION     DAVMEI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.323"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            354..>513
                     /locus_tag="VYQ24_002073"
     CDS             354..>513
                     /locus_tag="VYQ24_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969137.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT          196 a           76 c          104 g          137 t
ORIGIN      
        1 ataactttcg ataaattttg aaccttaaca attgaataga ccaagacaag acgttaatta
       61 tttttggagc gtaataacct atccgtcaag atctttcggc tgaataattc ggcaaaaaaa
      121 gttctgctaa gcaaaaagaa agtgagcgat aagtaagagt ttgaatatag ggagggatac
      181 cctatccgct atgaagaaaa tgaggataat gatacttcta aggttgaagc cggctcatcc
      241 ggaatagagg cggaggtgag attcctatgt tgctaatcca aagcacttgt caatgtcaaa
      301 aaacttaatt aaacaaataa ttattagata taaaacttta gaggaggtat atattggaca
      361 atgactggaa tggattggct gatttaatag caaatctgat tgcaaaatat gctggtgctt
      421 tagatttaga taatctaccc gatccaacgc cagcaaaaaa tcaagagatg aaaaatagtt
      481 ttgacatggc aaaaacacaa attgagacgg act
//
LOCUS       DAVMEI010000315          511 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.324, whole genome shotgun sequence.
ACCESSION   DAVMEI010000315 DAVMEI010000000
VERSION     DAVMEI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.324"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>511
                     /locus_tag="VYQ24_002074"
     CDS             <1..>511
                     /locus_tag="VYQ24_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1969138.1"
                     /translation="GYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDEYM
                     PDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGT
                     SFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGIT
                     GVTQKTINAD"
BASE COUNT          188 a           58 c          104 g          161 t
ORIGIN      
        1 tggctatacc aatcatagaa ttaagttaat tttaagtaat gaagatttag gtgcaactaa
       61 tgccagaaac ataggtatca aaaattctag aggtaagtat atatcatttt tagacgatga
      121 tgatgaatat atgccagatc gaattttaaa gttgatggct tgttttaaaa agagtagaat
      181 gaagaattta gctttagttt atagttatgg aataataatt tatccaaatg gtacacgaga
      241 agaggagaag accgattttg ttggaaatcc cttgtttgtt caaatggttc acaatatagc
      301 aggtacgtca ttttggttgt gtaaaaaaga ggtgctagaa ttaattaatg gttttgagaa
      361 aatagattca catcaggacg gtgttgtttt attaaaacta cttgctcaag gataccaaat
      421 tgatatagtg cgagaattct tggtgaatta ctacgctcac agtaaagaaa acggtatcac
      481 tggagtgaca caaaaaacaa ttaatgcaga t
//
LOCUS       DAVMEI010000316          501 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.325, whole genome shotgun sequence.
ACCESSION   DAVMEI010000316 DAVMEI010000000
VERSION     DAVMEI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.325"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..>501
                     /locus_tag="VYQ24_002075"
     CDS             1..>501
                     /locus_tag="VYQ24_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1969139.1"
                     /translation="MGDESYSFSTLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWS
                     FGLAGFIFQTMLRNPLASPDIIGVTSSSSITAVFCILVLKTNSLTTGIISITCGLTSS
                     LILFLLAKKDGFSAARLIILGIGFQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFT
                     KLDDIPL"
BASE COUNT          157 a           72 c           89 g          183 t
ORIGIN      
        1 atcggagatg aaagttattc tttttcaact ttgattaaag tcttaaatag tgaaactgtt
       61 cctggagcta gtttttcgat tatggaaatt agattaccaa aattattagc aggaattata
      121 gctggctggt cttttggatt ggcaggattt atctttcaaa ctatgttaag aaatcctctt
      181 gcaagtcctg atataatcgg tgtcacaagt tcttcatcta ttacagcggt cttttgcata
      241 ttggtattaa aaacaaatag tttaactact ggaattattt caataacttg tggactaaca
      301 tcatctttaa tattattttt actagctaaa aaagatggtt tttcagcagc aagactgata
      361 atattaggta ttggttttca agctgtcaca agagcaggca cctcattttt attgttgaaa
      421 gtagcaagat atgaattaca agaagttatg agatggctca gtggctcttt atcttttaca
      481 aagttagatg acatacctct t
//
LOCUS       DAVMEI010000317          500 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.326, whole genome shotgun sequence.
ACCESSION   DAVMEI010000317 DAVMEI010000000
VERSION     DAVMEI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.326"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..415
                     /locus_tag="VYQ24_002076"
     CDS             <1..415
                     /locus_tag="VYQ24_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1969140.1"
                     /translation="EEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLYK
                     PSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIADAEALLEKVTDPSIRQNAMETLTG
                     LKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIP"
BASE COUNT          189 a           85 c          106 g          120 t
ORIGIN      
        1 cgaggagaaa cctcagacag aaaaacctga ggaagataag gaacatgatg aagtaagtga
       61 gccaactcac cctgaatctg atgaaaaaga gaatcacgct ggtttaaatc cttcagcaga
      121 taatctttat aaaccaagca ctgatacgga agagacagag gaagaagctg aagatactac
      181 agatgaggct gaaattcctc aagtagagca ttctgttatt aacgctaaga tagcagatgc
      241 ggaggccttg ctagaaaaag taacagatcc tagtattaga caaaatgcta tggagacatt
      301 gactggtcta aaaagtagtc ttcttctcgg aacgaaagat aataacacta tttcagcaga
      361 agtagatagt ctcttggctt tgttaaaaaa aagtcaaccg gctccgatac cgtagtaaaa
      421 tgaatggagc atattttatg gagaagtaac ctttcgtgtt acttctcttt tttagaaaaa
      481 cgtaacagaa acttgacaaa
//
LOCUS       DAVMEI010000318          492 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.327, whole genome shotgun sequence.
ACCESSION   DAVMEI010000318 DAVMEI010000000
VERSION     DAVMEI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.327"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(77..388)
                     /locus_tag="VYQ24_002077"
     CDS             complement(77..388)
                     /locus_tag="VYQ24_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969141.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(403..>492)
                     /locus_tag="VYQ24_002078"
     CDS             complement(403..>492)
                     /locus_tag="VYQ24_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1969142.1"
                     /translation="VQAGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          150 a           93 c           78 g          171 t
ORIGIN      
        1 gcgttttatt gtgggaagat ttacttcatt ttctcttgaa attgagtttt ctcctacttc
       61 aaaactagat gatttgctat ttaaaatagc gttccttatg attttggcaa cctggattaa
      121 agcgagaaaa acaaaagggg caacttaaac tttctttata ttgcttatat gttagtagtt
      181 ttagacaaac accacataaa ataggcttat cctgtatcaa agataaggga tacggctcaa
      241 aagggtgatt ttctaatgaa tcatgacacc gataacaagc ataatacttt ttacaatcat
      301 agcattgtaa agcaataata tccagcttgc tatggtaatg aatgcatctt gtttgcttat
      361 ccactaagaa accctgtgcc tctatcattt ccttttcctt tcttaagctc gaactgtgac
      421 gctggttcca tcttccttat aaaaattgat gagtccttct tttaatgcac gaaccatgtc
      481 tccagcttga ac
//
LOCUS       DAVMEI010000319          491 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.328, whole genome shotgun sequence.
ACCESSION   DAVMEI010000319 DAVMEI010000000
VERSION     DAVMEI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.328"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            19..198
                     /locus_tag="VYQ24_002079"
     CDS             19..198
                     /locus_tag="VYQ24_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969143.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
BASE COUNT          163 a           94 c           88 g          146 t
ORIGIN      
        1 ataaaaagga caaataagat gagagaagtt attcaagaat tgttagacag ttcgatgtct
       61 acatctgcta tttcccaagg tgctggagtt ccatggacta ctgtttctga cctcagaaaa
      121 ggaaaaacaa gcatggacaa aatggcactt ctcacagcag aaaaacttta tgaatttgct
      181 acaactgata agcagtgatt tcggtcactg tttttatttt tgacaaaaca ccgtttttga
      241 caataatcaa aaaataaaaa ggagctatta ttaacaaaat ggcgtttttg gcaataatac
      301 cctgccattc ctcctcccta ttcttcaaga aaacgctttt ttgaagaatc agagcaaata
      361 aaaaaccgca agcctgagcc tgcggtgaaa gaacaattta gaaagtttcc tttctattta
      421 tttaactgta atcaagccat ctggctctac tgtgaactct ggcttgtctg ccagtgttcc
      481 gtctggtttg a
//
LOCUS       DAVMEI010000320          489 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.329, whole genome shotgun sequence.
ACCESSION   DAVMEI010000320 DAVMEI010000000
VERSION     DAVMEI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.329"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..363
                     /locus_tag="VYQ24_002080"
     CDS             <1..363
                     /locus_tag="VYQ24_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969144.1"
                     /translation="KFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKIDAYVVNNHEV
                     KAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVWHFLCHLDPMF
                     VEIEDVEGYVKEEVGEIQ"
BASE COUNT          181 a           63 c          111 g          134 t
ORIGIN      
        1 aaatttatgt tcgatcctga ggaaaatgta aacattattt ggaaagagat ttcaaattta
       61 tccctcaaaa atgatagagt gatcgaagga tattcaaaaa ttgatgcata tgtcgttaac
      121 aaccatgaag ttaaagccta tgtcgaaaca agagaaacga actatcgtaa agcaaaagac
      181 ttcttagaaa gccgcggata tgaaatcgac agaagctttt tcggaagtga agacggtgaa
      241 gcgattcttt acagaaaaaa agggatcgaa gtttggcatt tcttgtgtca cttagaccca
      301 atgtttgtag agattgaaga tgtagaagga tatgttaaag aagaagttgg agagattcaa
      361 tagtcaacca aagttccttg tgagtatata cctttgctag taaaagagtc aaaacgttat
      421 aacgaagggg ggaagaaaac ccttgagtgt aagtcttttt cttgtttaga gggaaagtat
      481 tagagtagc
//
LOCUS       DAVMEI010000321          486 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.330, whole genome shotgun sequence.
ACCESSION   DAVMEI010000321 DAVMEI010000000
VERSION     DAVMEI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.330"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            55..405
                     /locus_tag="VYQ24_002081"
     CDS             55..405
                     /locus_tag="VYQ24_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969145.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGVHKGRCRN"
BASE COUNT          156 a           62 c           88 g          180 t
ORIGIN      
        1 ataacattta atagaacgaa actaaagaaa acaatttaaa aaaggaggct tgtgatggaa
       61 cacttgttta aaatgataat cttattaccc tgtttttact ttttcagctg gattgacaag
      121 gataatagag aaagtaaatt tttcccaatt ttttactatt tttactggat ttacatcacc
      181 ctctatgctc tttttagcct tgcttggaca gttttttcag ttctgttttt caatatcgtc
      241 ttgagaaatt tgacagatat caagttatgg ggtatttggc tattattact acttatcgct
      301 tttgctagtg actggttaga ctatatctgt ttcaaaaaaa tgcttgactt gagacgggaa
      361 ctagggaaat ctaaaggtgt gcataaagga aggtgtagga actaaaaaat gactgtatct
      421 atgatttgtt tatggagcta tagatacagt catttttatt ttgattatga aacttgagag
      481 aaatat
//
LOCUS       DAVMEI010000322          476 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.331, whole genome shotgun sequence.
ACCESSION   DAVMEI010000322 DAVMEI010000000
VERSION     DAVMEI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.331"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(188..275)
                     /locus_tag="VYQ24_002082"
     tRNA            complement(188..275)
                     /locus_tag="VYQ24_002082"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(239..241),aa:Ser,seq:gct)
     gene            complement(282..355)
                     /locus_tag="VYQ24_002083"
     tRNA            complement(282..355)
                     /locus_tag="VYQ24_002083"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Ile,seq:gat)
     gene            complement(391..461)
                     /locus_tag="VYQ24_002084"
     tRNA            complement(391..461)
                     /locus_tag="VYQ24_002084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(427..429),aa:Gly,seq:tcc)
BASE COUNT          158 a          115 c           85 g          118 t
ORIGIN      
        1 aactttgaaa acttcaaaaa acggattttc atcgctctga acatcaaaaa agaaaggacg
       61 aaatttgtcc tttctcgagc ttagcttttc ttcaacccac tacagttgac aaagagcctt
      121 aagtttacgg atctgaagtt ttattgtctc taaaaacaaa aaagagagtt acaaactctc
      181 attaaaacgg agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag
      241 caaaccgtcc tcttcagcct cttgagtaat tctccaatta atgggcacga gtggactcga
      301 accaccgacc tcacgcttat caggcgtgcg ctctaaccac ctgagctacg cgcccaagtt
      361 aaaaaacttg gtaatttgaa caaagttcaa agcgggtgac gagaatcgaa ctcgcgacaa
      421 cagcttggaa ggctgtagtt ttaccactaa actacacccg cataaatact atcaat
//
LOCUS       DAVMEI010000323          470 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.332, whole genome shotgun sequence.
ACCESSION   DAVMEI010000323 DAVMEI010000000
VERSION     DAVMEI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.332"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>470
                     /locus_tag="VYQ24_002085"
     CDS             <1..>470
                     /locus_tag="VYQ24_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEV1969146.1"
                     /translation="LSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTY
                     SATVNVYGNKDGKPDLDNIVATKKVTININGLISKETVQKAVADNVKDSIDVPAAYLE
                     KAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLG"
BASE COUNT          161 a          110 c           97 g          102 t
ORIGIN      
        1 ctatctccac taggtgaaaa cgtgaagacc aaaggtcaat acttctatca agtagccttg
       61 gacggaaatg tagctggcaa agaaaaacaa gcgctcattg accagttccg agcaaacggt
      121 actcaaactt acagcgctac agtcaatgtc tatggtaaca aagacggtaa accagacttg
      181 gacaacatcg tagcaactaa aaaagtcact attaacataa acggtttaat ttctaaagaa
      241 acagttcaaa aagccgttgc agacaacgtt aaagacagta tcgatgttcc agcagcctac
      301 ctagaaaaag ccaagggtga aggtccattc acagcaggtg tcaaccatgt gattccatac
      361 gaactcttcg caggtgatgg tatgttgact cgtctcttgc tcaaggcatc tgacaaggca
      421 ccatggtcag ataacggtga cgctaaaaac ccagccctat ctccactagg
//
LOCUS       DAVMEI010000324          470 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.333, whole genome shotgun sequence.
ACCESSION   DAVMEI010000324 DAVMEI010000000
VERSION     DAVMEI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.333"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>470
                     /locus_tag="VYQ24_002086"
     CDS             <1..>470
                     /locus_tag="VYQ24_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1969147.1"
                     /translation="EAGFDKLIEIKRLTASKIQDILSVAPRSIGTTSPAREFEIIEII
                     KHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILAEIRNIHAFDNPAQLQAF
                     AGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARFSPAFKAYLKTKLE"
BASE COUNT          126 a          102 c          106 g          136 t
ORIGIN      
        1 ttgaggcagg atttgataag ctgatagaaa ttaagcgatt gaccgcttct aaaattcagg
       61 atatcctctc agtcgcacct cgttctatcg gaacaacatc tcctgctcgt gaattcgaaa
      121 tcatcgaaat catcaaacat tacaagaggc tcattgacaa ggcggaaaca tgtgtcaatg
      181 acttgatggc tgagttcaac tcggtcatca cgacggttac tgggattggg ggtcgtttag
      241 gggcggtcat tttagccgag attcgaaata ttcatgcctt tgataatcct gctcaattac
      301 aagctttcgc tggactggat tcttctattt atcagtcagg tcagattgat ttagctggaa
      361 gaatgatcaa acggggttcc cctcatctgc ggtgggcact catacaagct gccaaagcat
      421 gcgctcgctt ttcacctgct tttaaggcct atcttaagac taagttagaa
//
LOCUS       DAVMEI010000325          467 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.334, whole genome shotgun sequence.
ACCESSION   DAVMEI010000325 DAVMEI010000000
VERSION     DAVMEI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.334"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(137..>467)
                     /locus_tag="VYQ24_002087"
     CDS             complement(137..>467)
                     /locus_tag="VYQ24_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1969148.1"
                     /translation="EEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEESPEESEEPQ
                     VETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLL
                     ALLKESK"
BASE COUNT           90 a          101 c           79 g          197 t
ORIGIN      
        1 tactattcta gtttcaatct actatatttt gcacattttc atgaaaaaaa tgagaactag
       61 aactcacatt ctgctctcat ttttcgtttt cccgttctcc tatcctgttt ttaggagtta
      121 gaaaatgctg ctacctttac ttactctcct ttaataaagc caatagtttt tcagcttctg
      181 ccataatagt attgttgtcc tgggtgccaa atagtaaatt attttttaat cctgtgagag
      241 tctctttggc attggacttg ataattggat cctggatttt tccaagtaaa tcttcagcct
      301 ctctcagttt ttcttcaacc ttttcagtct cgacctgagg ttcttctgat tcctctggtg
      361 attcttctgg ttcctctgtt ggttttggag actctggttt ctcgctttgc ggtttctctt
      421 ctcgaggggt ttcttcctca ggtttttctg tctgaggttt ctcctcg
//
LOCUS       DAVMEI010000326          460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.335, whole genome shotgun sequence.
ACCESSION   DAVMEI010000326 DAVMEI010000000
VERSION     DAVMEI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.335"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>460
                     /locus_tag="VYQ24_002088"
     CDS             <1..>460
                     /locus_tag="VYQ24_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1969149.1"
                     /translation="KRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFIK
                     ELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRYR
                     QNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNV"
BASE COUNT          203 a           64 c           93 g          100 t
ORIGIN      
        1 acaaaaggat aaaaaggtta aggagaatat ctggaggaga tacctatacg ataatctacc
       61 taaaacttct actactaagc ctaaaagatg agggcaaact ctattatgac ggagtagaaa
      121 gtgattttat aaaagagtta gcactaacca tagatgaaac agacgatgat gtaatggtaa
      181 cagtaaatta tttaatcaat caaggcttat tagaagttgt aacagaaaat gatgaatact
      241 acctaactga aataccaaac ttaatcggat cagaaacagc ctgggcagag aaaaaaagaa
      301 gatacagaca aaataaacag aggacattgt ccttaatgag tccaacccat gtccgacaag
      361 agatagagat agagaaagat atagagatag ataaagagag agagggagag atagataaaa
      421 agtccacccc cattttttat ggagaattca aaaatgtaag
//
LOCUS       DAVMEI010000327          447 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.337, whole genome shotgun sequence.
ACCESSION   DAVMEI010000327 DAVMEI010000000
VERSION     DAVMEI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.337"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          147 a           85 c           78 g          137 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga tcagcatttc ctagccaata ggacgctcca aatcacctat tctaagtcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtggt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta catctct
//
LOCUS       DAVMEI010000328          444 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.338, whole genome shotgun sequence.
ACCESSION   DAVMEI010000328 DAVMEI010000000
VERSION     DAVMEI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.338"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          149 a           85 c           52 g          158 t
ORIGIN      
        1 attttcttac ttccttttta aatccgataa tatataggtt ttgctagcct ctacaccata
       61 aatatttgct aataaaacta ctgtattatt accctcttaa ggtgcgaaat tattcaagtt
      121 cttaaaaaat gcttctctat ttccgtaaga tagcttattt cttcgattta agaactgaag
      181 atgatagttc taacctttag aaatccaagc cttcactagc tcaaaaactc ctatctttct
      241 tcctcaataa atcatctgag agataggtat ttcttatatc cttcaaattc atcactcctt
      301 tggatattga ggtgctagcc tagaagcaga cacccgatta tctaaaccat ccttaataga
      361 gatgacaaga cagataagct cagttaatta cttatatatc tttttaatat ggtatatttg
      421 tcaacaaaaa gaaatcaaat cata
//
LOCUS       DAVMEI010000329          440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.339, whole genome shotgun sequence.
ACCESSION   DAVMEI010000329 DAVMEI010000000
VERSION     DAVMEI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.339"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(126..392)
                     /locus_tag="VYQ24_002089"
                     /pseudo
     CDS             complement(126..392)
                     /locus_tag="VYQ24_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
BASE COUNT          128 a           89 c           70 g          153 t
ORIGIN      
        1 tagcgtacta ttctaaattt cttaatctac tatagaatca agaaatctac caccttcttt
       61 aaataccctc cattatcaca taaacaggta aacttttcaa ttaatgactg cgcttttcaa
      121 tcacgctaga ggtacttgct tgcttctttg atactaagtt cagccattct ttccttgttt
      181 ttctcaataa agcatgttac ccaagtggga ttcgttttgg agtagtctcg cagagtccag
      241 ccaatggctt tattgataaa aaattctgtt tggttcaagt tatgaagtag aatcttttcc
      301 attaattgag tattggtctt ctcttttctt aacaactggt ggtcaatagc gacacgtctc
      361 agccagatat tatctgatag gctccatttt atactcaatg aaaatcaaag agcaaactag
      421 gaagctagcc gcagattgct
//
LOCUS       DAVMEI010000330          424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.340, whole genome shotgun sequence.
ACCESSION   DAVMEI010000330 DAVMEI010000000
VERSION     DAVMEI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.340"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..232
                     /locus_tag="VYQ24_002090"
     CDS             <1..232
                     /locus_tag="VYQ24_002090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969150.1"
                     /translation="EIQRVQQSPSVFLIFSTENKAFVSRFTLTVLKQIEQGFSIEEIM
                     KNYPFSETLLDSIAKSIAILTRNLFIEVDYEK"
BASE COUNT          145 a           57 c           66 g          156 t
ORIGIN      
        1 tgaaattcaa agagttcaac aaagtccgtc tgtattttta atattttcaa ctgaaaataa
       61 agcttttgta agtaggttta cattaactgt tttaaaacaa attgaacaag gtttttcaat
      121 tgaggagata atgaaaaatt atccttttag tgaaacgttg ttggatagta tagctaaatc
      181 aattgctatt cttacaagaa atttgtttat agaggtagac tatgaaaaat aaatctcatc
      241 aacaaatttt actcagaagt ttattaactt tgataagtca actgggaagt tcagcatttg
      301 ctatgggagt ctcacttttc atttttcaaa aaactgatag ttcaatttct tttgggctta
      361 atatgattgc agctccgctt gtcacagttt tactttcacc cataattggt aagataatag
      421 attc
//
LOCUS       DAVMEI010000331          423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.341, whole genome shotgun sequence.
ACCESSION   DAVMEI010000331 DAVMEI010000000
VERSION     DAVMEI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.341"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..313
                     /locus_tag="VYQ24_002091"
     CDS             <1..313
                     /locus_tag="VYQ24_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1969151.1"
                     /translation="EILTSQKTLTEFDKDIFDSIVEKIIVGGINSDGEIDPAMLTIIF
                     KTGDSSTKDAKSYKSKRKNAKIENDKLCFNTVTDDEKKCTNKENNARRDGSTFVQTRC
                     R"
BASE COUNT          172 a           54 c           86 g          111 t
ORIGIN      
        1 agaaatttta acttcacaaa agactctgac ggagtttgac aaggatattt ttgatagtat
       61 cgttgaaaaa atcatagtag gcggaataaa ttctgatgga gaaattgatc cagcaatgtt
      121 gactataatt tttaaaacgg gagattcttc gactaaagat gcaaaatctt acaaatctaa
      181 aaggaagaat gctaaaatag aaaatgataa attgtgtttt aataccgtaa ccgatgacga
      241 aaaaaagtgt acaaataaag aaaacaacgc acgtcgagac ggtagcactt ttgtccaaac
      301 tcgatgtcga taagcacata agtgttgaaa ttgagctgga tgagatggat ttgacaagtg
      361 cggagagcaa agcaacatat gctcaaatca aagaatatgt ttggaataaa tttgaattaa
      421 aag
//
LOCUS       DAVMEI010000332          420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.343, whole genome shotgun sequence.
ACCESSION   DAVMEI010000332 DAVMEI010000000
VERSION     DAVMEI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.343"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..242)
                     /locus_tag="VYQ24_002092"
     CDS             complement(<1..242)
                     /locus_tag="VYQ24_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1969152.1"
                     /translation="MAAGYLNRVNDIWLENEENVENGLKKCSLEGRIDIKDLSFSYSK
                     DSAPVIENLNLTIEPGQRIALVGQSGSGKSTLSKILS"
     gene            complement(252..420)
                     /locus_tag="VYQ24_002093"
                     /pseudo
     CDS             complement(252..420)
                     /locus_tag="VYQ24_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          144 a           76 c           56 g          144 t
ORIGIN      
        1 gatagtattt ttgataacgt actttttcct gaccccgatt gaccgactag tgcaattctc
       61 tgtcctggct caatagtcaa atttaaattt tctattacag gagctgagtc tttggaataa
      121 gagaaagata ggtccttaat atctattcta ccttctaaag aacacttttt aagaccattt
      181 tcaacattct cttcattttc aagccagata tcatttactc tatttaagta tcctgctgcc
      241 aaaatatact gcgtataagc attaaatatt gagatttctg aactaaataa aattgatgcc
      301 atggtttgaa atgcgacaac ctcacccaat aaaatttttc catttaaaac attatcaagt
      361 ccaaaaatca atataattat aggagcgaac aattgaaata cattagtagc tgaattgtat
//
LOCUS       DAVMEI010000333          415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.344, whole genome shotgun sequence.
ACCESSION   DAVMEI010000333 DAVMEI010000000
VERSION     DAVMEI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.344"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..292
                     /gene="tnpB"
                     /locus_tag="VYQ24_002094"
     CDS             <1..292
                     /gene="tnpB"
                     /locus_tag="VYQ24_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1969153.1"
                     /translation="HLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQVFLFCGGRKDRF
                     KALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFSITPQI"
BASE COUNT          117 a           83 c           81 g          134 t
ORIGIN      
        1 tcaccttgtt tgtggcaaga cagatatgat acaaggaatc gattcactgg cttatctcgt
       61 taaaacccac tttgaattgg atcctttctc cggtcaagtc tttctctttt gtggtggacg
      121 taaagaccgc tttaaagccc tttactggga tggtcaagga ttttggatac tatataaacg
      181 ctttgagaac ggaaaactga cttggcccag tacagaaaag gatgtcaaag ctctcgcacc
      241 tgaacaagta gactggctta tgaagggctt ttctatcact ccccaaatat agtggattta
      301 aactagaata gtacacccct acttctaaaa cattgttaga aatcgatttg actgtcctga
      361 tcaatttgtc ctgttcttgt ttcattttac tatagaacga tttgaaggcg tttat
//
LOCUS       DAVMEI010000334          414 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.345, whole genome shotgun sequence.
ACCESSION   DAVMEI010000334 DAVMEI010000000
VERSION     DAVMEI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.345"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>414
                     /locus_tag="VYQ24_002095"
     CDS             <1..>414
                     /locus_tag="VYQ24_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1969154.1"
                     /translation="YQAGQDKKESNRVAYIDGDQAGQKAENLTPDEVSKREGINAEQI
                     VIKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNYQLKDSDIVNEIKGGYVI
                     KVNGKYYVYLKDAAHADNIRTKEEIKRQKQERSHN"
BASE COUNT          145 a           68 c           98 g          103 t
ORIGIN      
        1 ttaccaagct ggtcaggata agaaagagtc taatcgagtt gcttatatag atggtgatca
       61 ggctggtcaa aaggcagaaa acttgacacc agatgaagtc agtaagaggg aggggatcaa
      121 cgccgaacaa attgttatca agattacgga tcaaggttat gtgacctctc atggagacca
      181 ttatcattac tataatggca aggttcctta tgatgccatc atcagtgaag agctcctcat
      241 gaaagatccg aattatcagt tgaaggattc agacattgtc aatgaaatca agggtggtta
      301 tgtcattaag gtaaacggta aatactatgt ttaccttaag gatgcagctc atgcggataa
      361 tattcggaca aaagaagaga ttaaacgtca gaagcaggaa cgcagtcata atca
//
LOCUS       DAVMEI010000335          410 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.346, whole genome shotgun sequence.
ACCESSION   DAVMEI010000335 DAVMEI010000000
VERSION     DAVMEI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.346"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(112..387)
                     /locus_tag="VYQ24_002096"
     CDS             complement(112..387)
                     /locus_tag="VYQ24_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1969155.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
BASE COUNT          114 a           86 c           65 g          145 t
ORIGIN      
        1 aatattttcg gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa
       61 gcctactgac caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac
      121 agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat
      181 ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt
      241 accaaaaccg atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa
      301 tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt
      361 agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt
//
LOCUS       DAVMEI010000336          398 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.348, whole genome shotgun sequence.
ACCESSION   DAVMEI010000336 DAVMEI010000000
VERSION     DAVMEI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.348"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            184..>398
                     /gene="blpU"
                     /locus_tag="VYQ24_002097"
     CDS             184..>398
                     /gene="blpU"
                     /locus_tag="VYQ24_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1969156.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAA"
BASE COUNT          147 a           49 c           97 g          105 t
ORIGIN      
        1 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
       61 ctaattcaag acgtttcgat gccaattcaa gatttggatg aaaaaaatta atagatactg
      121 ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa agaggtattc
      181 gttatgaata caaaaacgat gtcacaattt gaaattatgg atactgagat gcttgcttgc
      241 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggaggagca
      301 gcacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc aactggtgct
      361 gtgggaggag ctatacttgg aggtgtggcc tatgcagc
//
LOCUS       DAVMEI010000337          395 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.349, whole genome shotgun sequence.
ACCESSION   DAVMEI010000337 DAVMEI010000000
VERSION     DAVMEI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.349"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            140..316
                     /locus_tag="VYQ24_002098"
                     /pseudo
     CDS             140..316
                     /locus_tag="VYQ24_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          166 a           66 c           66 g           97 t
ORIGIN      
        1 ttaatccgtg ttaaaaaaga aattaaagtg ggttaaaaaa gaaattaact gagtaaaaac
       61 tctcgaaatt caattcgcta tattatcact caacttgaac aaataccaga accacaaaaa
      121 acagatgtaa aggaaaaata tggacttaaa atttgaaggg gttgatttag aatacaaaaa
      181 agctaaaaac aatcttccag agtctttctg ggaaacctac tcagcatttg caaatacaaa
      241 tggcgggaaa ataattctcg ggatagatga aaaaaacatt gacacctacc aaagagtcaa
      301 tagactccct gcgaaacaaa attatgaagc aagtaaacaa ctaactgatg cacgatttaa
      361 gctccttgtt ggtgttcagc gtaccacttt tgaag
//
LOCUS       DAVMEI010000338          390 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.350, whole genome shotgun sequence.
ACCESSION   DAVMEI010000338 DAVMEI010000000
VERSION     DAVMEI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.350"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          141 a           67 c           47 g          135 t
ORIGIN      
        1 attcatacac ctctcataaa aagaatagca cactcttgtc ataatttttt aaataaaaaa
       61 attatgacaa aacaaggaag caatttattg atgctgctta aaaatctaaa attgatgagt
      121 tcctatcaaa aatcgtatct tcaacctcaa aacagtactt aaagctatcc gactcggttt
      181 acattgtcaa atttagattt tatttgagca taactttcta gtttgctttc tgatttttgt
      241 ttaatatagt agcaaacatt gaaggaaata tctccacaaa aaaacgcata ctattaagct
      301 ttttcaagac ctaataatat gcgctgttct gatttgaaag acattccatt attattttac
      361 tgtaatcaag ccatctggct ctactgtgaa
//
LOCUS       DAVMEI010000339          388 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.351, whole genome shotgun sequence.
ACCESSION   DAVMEI010000339 DAVMEI010000000
VERSION     DAVMEI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.351"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..373
                     /locus_tag="VYQ24_002099"
                     /pseudo
     CDS             1..373
                     /locus_tag="VYQ24_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          154 a           62 c           79 g           93 t
ORIGIN      
        1 gagagtacct gagagcggag aatgccatcc taaaaaagtt aagagaactc cgattgaagg
       61 aggaaaaaga gaaagaagaa agatagaaat tgttcaagaa ttaatgactg agttttcgtt
      121 agatcttctt ctaaaagcca ttaaactagc tcgttggacc tactactatc acttgaaaca
      181 gctagacaaa ccagataagg accaagagct taaagctgaa attcaatcca tttttatcga
      241 acacaaggga aattatactt atcgccgggt tcatttagaa ctaagaaatc gtgcttatct
      301 ggtaaatcat aaaagagttc aaggcttgat gaaagtactc aatttacaag ctaaaatgcg
      361 acagaaacga aaataaagga gacgttgg
//
LOCUS       DAVMEI010000340          386 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.352, whole genome shotgun sequence.
ACCESSION   DAVMEI010000340 DAVMEI010000000
VERSION     DAVMEI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.352"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>386
                     /locus_tag="VYQ24_002100"
     CDS             <1..>386
                     /locus_tag="VYQ24_002100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969157.1"
                     /translation="DKFVLKNLLKGEKAVTVNSLGDIDPENEQIRKFCFVEEGLVEFT
                     KSAFQKLENFFKTQKTALGYIETTSSCPYHCKICPKGFNQQIRNQRDLQIEQFDSIVK
                     QIEGQSNLALHLFGDPLYDKDIYRKIR"
BASE COUNT          149 a           47 c           68 g          122 t
ORIGIN      
        1 gacaaatttg tattaaaaaa tttgttgaaa ggagagaaag cagttaccgt taatagtctt
       61 ggagatattg atccagaaaa tgaacaaatt agaaaattct gttttgttga agagggatta
      121 gttgaattta ctaaatcagc ttttcagaaa ttagaaaact tttttaaaac gcaaaaaact
      181 gcactaggtt atattgaaac aacatcaagt tgtccttatc attgtaaaat ttgccctaaa
      241 ggttttaatc agcagattag aaatcaacga gatttgcaaa tagagcagtt tgattcaatt
      301 gtaaaacaaa ttgaagggca gtcaaatttg gctttgcatc tatttggcga tccattatat
      361 gacaaagata tctatagaaa aattcg
//
LOCUS       DAVMEI010000341          382 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.356, whole genome shotgun sequence.
ACCESSION   DAVMEI010000341 DAVMEI010000000
VERSION     DAVMEI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.356"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>382)
                     /locus_tag="VYQ24_002101"
     CDS             complement(<1..>382)
                     /locus_tag="VYQ24_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV1969158.1"
                     /translation="AIISPEIFTQAQEIRLRRSGNKKTIANTKGKRERYSRMYPFSSR
                     LECGFCGTVLSRRSWHSSSEYKKIIWHCTTSIKKGKKHCIHSKGIEEKAIENAFLQSY
                     QQLFYENNNLTEDFLEIIKEELTDD"
BASE COUNT          102 a           74 c           51 g          155 t
ORIGIN      
        1 atcatcagta agctcttctt ttataatttc aagaaagtct tcagttaggt tattattttc
       61 ataaaacaac tgttggtagc tctgtaaaaa agcattttct attgcttttt cctctattcc
      121 tttactatgg atgcaatgct ttttaccttt ctttatagat gttgtacagt gccagataat
      181 ctttttgtac tctgaactag agtgccaact tcttcgagac agaacagtac cacaaaaacc
      241 acattccaat ctactactaa aaggatacat tcttgaatat ctttctcgtt ttccttttgt
      301 atttgctatt gttttcttgt ttcctgatct ccgtaatcga atttcttgcg cctgtgtaaa
      361 aatttctgga ctaattattg ca
//
LOCUS       DAVMEI010000342          377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.357, whole genome shotgun sequence.
ACCESSION   DAVMEI010000342 DAVMEI010000000
VERSION     DAVMEI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.357"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>377
                     /locus_tag="VYQ24_002102"
     CDS             <1..>377
                     /locus_tag="VYQ24_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1969159.1"
                     /translation="LFDQVATLLEEREIVTPTYREALITREKSFPTGLDMEFLGKDLP
                     NVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNIL
                     AQLMDFFTGNGHLEDLSKISEPE"
BASE COUNT          126 a           67 c           69 g          115 t
ORIGIN      
        1 ctctttgatc aggttgcaac tttattggaa gaacgagaaa ttgtaactcc aacttatcgt
       61 gaagccttga tcacgcgtga aaagtcattt ccaactggtt tagatatgga atttctagga
      121 aaggacttgc caaatgtagc gattcctcat acagatattg ttcataatct agctgagaaa
      181 gtggtggttg ttcgattaga aaaaccagta acttttcaca atatgatagc tcctgataag
      241 gaagtagaag tatccctact cttctttatt attaacaatt caagttcaag tcaaacaaat
      301 attctggctc agttgatgga ctttttcaca ggaaatggac atcttgaaga cctatcaaaa
      361 atttccgaac cagaaaa
//
LOCUS       DAVMEI010000343          377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.358, whole genome shotgun sequence.
ACCESSION   DAVMEI010000343 DAVMEI010000000
VERSION     DAVMEI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.358"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>377
                     /locus_tag="VYQ24_002103"
     CDS             <1..>377
                     /locus_tag="VYQ24_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1969160.1"
                     /translation="LFDQVATLLEEREIVTPTYREALITREKSFPTGLDMEFLGKDLP
                     NVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSSQTNIL
                     AQLMDFFTGNGHLEDLSKISEPEA"
BASE COUNT          118 a           70 c           73 g          116 t
ORIGIN      
        1 ctctttgatc aggttgcaac tttattggaa gaacgagaaa ttgtcactcc aacttatcgt
       61 gaagccttga tcacgcgtga aaagtcattt ccaactggtt tagatatgga atttctagga
      121 aaggacttgc caaatgtagc gattcctcac acagatattg ttcataattt ggctgaaaaa
      181 gttgtggtcg ttcgattaga gaaaccggtg actttccata atatgattgc gccagataag
      241 gaagtagaag tatccttgct cttctttatc attaataatt caagttcaag tcaaacgaac
      301 attcttgctc agttaatgga cttcttcaca ggaaatggtc atcttgaaga cctatcaaaa
      361 atttctgaac cagaagc
//
LOCUS       DAVMEI010000344          364 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.359, whole genome shotgun sequence.
ACCESSION   DAVMEI010000344 DAVMEI010000000
VERSION     DAVMEI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.359"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>364
                     /locus_tag="VYQ24_002104"
     CDS             <1..>364
                     /locus_tag="VYQ24_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1969161.1"
                     /translation="DDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDG
                     YIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQD
                     SGNTEAKGAEAIYNRVKAA"
BASE COUNT          117 a           79 c           85 g           83 t
ORIGIN      
        1 tggatgatat tcttgccttc ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa
       61 atgcgcaaat tacctacact gatgatgaga ttcaagtagc caagttggca ggcaagtaca
      121 caacagaaga cggttatatc tttgatcctc gtgatataac cagtgatgag ggggatgcct
      181 atgtaactcc acatatgacc catagccact ggattaaaaa agatagtttg tctgaagctg
      241 agagagcggc agcccaggct tatgctaaag agaaaggttt gacccctcct tcgacagacc
      301 atcaggattc aggaaatact gaggcaaaag gagcagaagc tatctacaac cgcgtgaaag
      361 cagc
//
LOCUS       DAVMEI010000345          360 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.360, whole genome shotgun sequence.
ACCESSION   DAVMEI010000345 DAVMEI010000000
VERSION     DAVMEI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.360"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          111 a           61 c           56 g          132 t
ORIGIN      
        1 ctaaatgtat agattaacca cctaattata gcatataacg cagattcctt tcaatcgtat
       61 gatttactgc attaaattaa gtaaaaaaat aaaggcagtc cgaagaccgc caatatttat
      121 ctctctgcac ctttgctatg gtctttctta tttgacttag atttgtcatc tgtcttaaag
      181 ctttttattt gccctaatat ggacttttta tccttgtctt gactctttac ttgttctttt
      241 gcttttaaga gcttagaaga caaaaagaga tattatatta aaacaatatt tctccggtac
      301 tgggactggg acgggactaa aaatctgaaa acgtcctata attaggtgga tttagtgata
//
LOCUS       DAVMEI010000346          359 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.361, whole genome shotgun sequence.
ACCESSION   DAVMEI010000346 DAVMEI010000000
VERSION     DAVMEI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.361"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          121 a           50 c           59 g          129 t
ORIGIN      
        1 ctagctaaaa aaatatccca atcttagatg gagtttccaa taagcaagtt gctccaacgt
       61 tgctgcatca cttttctttg gaaattgaaa agaattttat cgatttttgc tataaaaata
      121 tggatctcat cctaaaaact caaagtatat ctttgtgtca tagagaagag cttattattg
      181 tagatgtttc agatgactct aaatttggtg ttttttacaa gcgttttaga acactggttg
      241 ataaaaataa cggaaaatat gtttgtgttt ataatttcag tcgtgtttca gatatactgc
      301 aaaactggaa aaattattgc aatcgaaaat tttcggttac gtttactgaa agccaattt
//
LOCUS       DAVMEI010000347          350 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.365, whole genome shotgun sequence.
ACCESSION   DAVMEI010000347 DAVMEI010000000
VERSION     DAVMEI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.365"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>350
                     /locus_tag="VYQ24_002105"
     CDS             <1..>350
                     /locus_tag="VYQ24_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969162.1"
                     /translation="AFVPDKTNNEWFKEQTKAYIVEEKSTIKEVSDKQSIAGSPYSIG
                     VYDRLTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDY
                     VEKKAKEAAVRFRD"
BASE COUNT          135 a           46 c           69 g          100 t
ORIGIN      
        1 gcatttgtac cagataaaac aaataatgag tggtttaaag aacaaactaa agcatatatt
       61 gtagaagaaa aatctactat taaagaggta agtgataagc aatcaatagc tggtagtcca
      121 tattcaattg gagtatatga tagattaact agcccatctt ggaaatatcc aagtatggtg
      181 ttgccgctat taacattacc tgaaaaatcg gtgtttatta tagccaacat ttcgactata
      241 ggtttcggtg cttatgatag atatagaagt aaagaacatc cagcaggaac tgatttaaat
      301 gactatgttg agaaaaaagc taaagaagcg gctgttagat ttagagatca
//
LOCUS       DAVMEI010000348          347 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.366, whole genome shotgun sequence.
ACCESSION   DAVMEI010000348 DAVMEI010000000
VERSION     DAVMEI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.366"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            23..>347
                     /locus_tag="VYQ24_002106"
     CDS             23..>347
                     /locus_tag="VYQ24_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969163.1"
                     /translation="MENNHSEVQKNKLTIDKVPVQNKKLTEALRVFHETLNHSRLIEV
                     QNSMLQANKAAETALASFNFSKQIAETTKPFLTEINSATSILNNQAYLKILSETEAAV
                     SSILLK"
BASE COUNT          143 a           60 c           49 g           95 t
ORIGIN      
        1 acatatagaa agaaatagcc ctatggaaaa caatcatagt gaagttcaaa aaaacaaatt
       61 gacaattgat aaagtcccag ttcaaaacaa aaagcttaca gaagccctta gagtttttca
      121 tgagacactt aatcattcaa ggttgattga ggttcaaaat agtatgctac aagcaaataa
      181 agctgctgag actgcgttag caagttttaa tttttctaag caaatagctg aaaccactaa
      241 accttttcta acagaaataa actcagccac gtctatttta aataaccaag catatcttaa
      301 aattctatct gaaacagaag cagcagttag ttcaattctt ttaaaaa
//
LOCUS       DAVMEI010000349          346 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.367, whole genome shotgun sequence.
ACCESSION   DAVMEI010000349 DAVMEI010000000
VERSION     DAVMEI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.367"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            115..279
                     /locus_tag="VYQ24_002107"
     CDS             115..279
                     /locus_tag="VYQ24_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1969164.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT          105 a           46 c           80 g          115 t
ORIGIN      
        1 gaaattgatt ttttgatatt aaaataaaaa aggagggtag gcagtgttgt gatcaattat
       61 tgctggaggt cttattggtc tcttggcagg taaaatcact aaaaaagtag ttctatagga
      121 atcatcgcaa atgtattcgc tggtttagtc ggggcatatg caggacaatc tcttttaggt
      181 agttggggtc catcaatcgc tggaatggct ttgctcccat ctattgtagg tgcagcgatt
      241 gtgattactg tagtgtcatt ctttacaggt agaaagtaaa cttttcgcca gtaaagttag
      301 caaactattt ttaaatcaat gacgggaaaa atagtttaaa tgttaa
//
LOCUS       DAVMEI010000350          343 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.368, whole genome shotgun sequence.
ACCESSION   DAVMEI010000350 DAVMEI010000000
VERSION     DAVMEI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.368"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..314)
                     /locus_tag="VYQ24_002108"
     CDS             complement(<1..314)
                     /locus_tag="VYQ24_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969165.1"
                     /translation="MLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSLAFCSLILSKI
                     VLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSPDDLRSRIIWS
                     FIL"
BASE COUNT          142 a           61 c           48 g           92 t
ORIGIN      
        1 agaataaaac tccatataat cctagatctt aaatcatcag gcgacaaact tttacgatgg
       61 aatgaaaaat agggcgagaa cctcacattt actttcttcc tgcctataaa aataaaagga
      121 aaaatcgtaa taagagcttc tttcttgtac aaatctttca atgcgtatag tatgtctaaa
      181 actatttttg ataaaatcaa actacagaaa gcaagactcg tactaaccac aaaatcaagg
      241 ggaatttcta gattcaattg caagttactc actaacaaat gcaactctag ccacaagaca
      301 aaaagaggga gcattaataa aaaaatgatt agtttacgta tca
//
LOCUS       DAVMEI010000351          337 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.370, whole genome shotgun sequence.
ACCESSION   DAVMEI010000351 DAVMEI010000000
VERSION     DAVMEI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.370"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            41..>337
                     /locus_tag="VYQ24_002109"
     CDS             41..>337
                     /locus_tag="VYQ24_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV1969166.1"
                     /translation="MNETLEKANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDL
                     KKELNKLGVSFSVMKNKESKNYEIFFQAKGIKVMEYAFKQVIAKENKKEKESILK"
BASE COUNT          180 a           41 c           52 g           64 t
ORIGIN      
        1 aaaagaatca atcaatttag aaaaagaaat aagaaaaaag atgaatgaaa ctttagaaaa
       61 agcaaatgga aacttaaaaa gtcttatggg cgatgaaatg aaaataaaag atctctacaa
      121 gaaaggacaa ctagaaaata taagcataga tcaaatcgac ctcaaagact taaaaaaaga
      181 actaaacaaa cttggagtaa gtttctcagt aatgaaaaac aaagaaagca aaaactatga
      241 aatattcttc caagccaaag gcataaaagt aatggaatat gcctttaagc aagtcatagc
      301 caaggaaaat aaaaaagaaa aagaaagtat cctaaaa
//
LOCUS       DAVMEI010000352          337 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.371, whole genome shotgun sequence.
ACCESSION   DAVMEI010000352 DAVMEI010000000
VERSION     DAVMEI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.371"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          109 a           52 c           55 g          121 t
ORIGIN      
        1 ctagtgaaaa attgaatgag ccactattta tttaatatgt atgatgaggg aatgactcaa
       61 aaacttcttg tcgatataag aatatctggg atagtatttt aaaacgaaag gactataaaa
      121 gctcatttcc ttcgcactta ttttatagag atctatcatt atactcttta gaaatcaaaa
      181 agcaacacac cttgtcatcg ttttatcgta aatctgttaa tgagggggct agtcttatct
      241 gcaatctcaa atcagtgttt tgagctgaat ggcactcgtt ttctagtttg ctctttgatt
      301 ttaattgggc cttagtaaat actactctta aattaaa
//
LOCUS       DAVMEI010000353          332 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.372, whole genome shotgun sequence.
ACCESSION   DAVMEI010000353 DAVMEI010000000
VERSION     DAVMEI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.372"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(135..>332)
                     /locus_tag="VYQ24_002110"
     CDS             complement(135..>332)
                     /locus_tag="VYQ24_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969167.1"
                     /translation="ESGSMATGWVKDKGLWYYLNESGSMATGWFTVSGKWYYTYNSGD
                     LLVNTTTPDGYRVNANGEWVG"
BASE COUNT          108 a           67 c           46 g          111 t
ORIGIN      
        1 atccttatat atccattctt aaaattggtt taaatagcgt agtcttttaa actagtttta
       61 agaatccaaa aaatcttcct acatatgtaa gaagattttt tagttcagaa tgattagatt
      121 tagctaatgg atacctatcc tacccactca ccgttagcat tgactcgata gccatcgggt
      181 gtagtcgtgt ttactaataa atctcctgaa ttataggtat agtaccattt accagaaact
      241 gtaaaccaac cagtagccat tgaacctgat tcatttaagt aataccataa gcctttgtct
      301 ttaacccaac cagtagccat tgaacctgat tc
//
LOCUS       DAVMEI010000354          331 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.373, whole genome shotgun sequence.
ACCESSION   DAVMEI010000354 DAVMEI010000000
VERSION     DAVMEI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.373"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..298
                     /locus_tag="VYQ24_002111"
                     /pseudo
     CDS             1..298
                     /locus_tag="VYQ24_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          132 a           55 c           68 g           76 t
ORIGIN      
        1 gacgtaagcc aaaaaagaga cctgaagaga ggacagaatt agaacgtctt caagcagaaa
       61 atgagtacct gagagcggag aaggccatcc taaaaaagtt aagagaactc cgattgaagg
      121 aggaaaaaga gaaagaagaa agacagaaat tgtttaagaa ttaatgactg agttttcgtt
      181 agctcttctt ctaaaatcca ttaaactagc tcgttggacc tactactatc acttgaaaca
      241 gctagataaa ccagataagg accaagagct taaagctgaa attcaatcca tttttatcta
      301 acacaaggga aattatgctt atcgtcggat t
//
LOCUS       DAVMEI010000355          329 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.374, whole genome shotgun sequence.
ACCESSION   DAVMEI010000355 DAVMEI010000000
VERSION     DAVMEI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.374"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..329
                     /locus_tag="VYQ24_002112"
                     /pseudo
     CDS             1..329
                     /locus_tag="VYQ24_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          103 a           70 c           66 g           90 t
ORIGIN      
        1 actctctcct tcaagaaaca cgtggtggtc gtaaccatgc atatatgaca gttgaggaag
       61 agaaagcctt tcttgcccgc catttgaagg ctacagaggc aggagaattt gttacaatac
      121 cttatttcag gcttatatcg ttcctacaca cgtgatgcct tctatcaact gttgaagcgc
      181 catggttggc gaaatattat gccacgtcca gaacatccta agaaagcaga cgctcaaacc
      241 attgtcgcgt ctaaaaataa aatctcaatt caagaagaca agaaagcgtt ttaaatatag
      301 tagacgtttt cataaggttc gcttgatgt
//
LOCUS       DAVMEI010000356          324 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.375, whole genome shotgun sequence.
ACCESSION   DAVMEI010000356 DAVMEI010000000
VERSION     DAVMEI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.375"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(93..317)
                     /locus_tag="VYQ24_002113"
     CDS             complement(93..317)
                     /locus_tag="VYQ24_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1969168.1"
                     /translation="MLKRIRDLREDDDLTQEYIAKIVLNCTRSAYSKMEAGNRLISIN
                     DLIKLADFYKVSLDYLVGRVDNKEDHYSKK"
BASE COUNT           95 a           66 c           45 g          118 t
ORIGIN      
        1 gctcttatca tggcgagcca ttcaaatctg ctccacaaat gaggaaagta ccattttgga
       61 ttattcaatt gtcaatgtgc tttcttaacc aactactttt tggagtaatg gtcttcttta
      121 ttatccactc gacctacaag gtagtctaga ctcaccttat aaaaatctgc aagtttgata
      181 aggtcattta tagagattaa cctgttacca gcttccattt tagaataagc tgatcttgta
      241 caattcaaaa cgattttcgc aatatattct tgtgtcaaat catcatcttc acgtaaatct
      301 ctaatccttt tcaacatttg ttgt
//
LOCUS       DAVMEI010000357          319 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.376, whole genome shotgun sequence.
ACCESSION   DAVMEI010000357 DAVMEI010000000
VERSION     DAVMEI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.376"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(28..123)
                     /locus_tag="VYQ24_002114"
                     /pseudo
     CDS             complement(28..123)
                     /locus_tag="VYQ24_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          101 a           69 c           44 g          105 t
ORIGIN      
        1 tttcagattc acttttctat actaagatga gtagggagga aaaggtaaaa gtttatgccc
       61 aaactcttca cataagaact ctagcttacc cattctatgg aatcttgcat tatccattaa
      121 aacttccaga ctcataccct cctaaaatct attcaaacca cgtcagcttc acattatata
      181 tgttggttac ctcacaagtt ttatctgcaa cctcaaagct gttctttgag cagcctacag
      241 ctagcttcct agtttaatct ttgcttttca ctgagtatca agttaaaaaa tgaggctggt
      301 caaaaagtct ttaaaatta
//
LOCUS       DAVMEI010000358          314 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.378, whole genome shotgun sequence.
ACCESSION   DAVMEI010000358 DAVMEI010000000
VERSION     DAVMEI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.378"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT          109 a           61 c           46 g           98 t
ORIGIN      
        1 aaaatcaaag agtaagctag gaagctagcc gcaggttgct caaaacactg ttttgaggtt
       61 gtagataaga ctgacgaagt cagtgatcat atcgtaaccg cccaatagac ttcgttattg
      121 ggcggttacg aaaaacttcc ttccaaataa aaaggaatta agcaaccaaa cgctacttaa
      181 ttccttgaaa ttgtctcatc acctctatta gcctttaaat tttgatagac tttattttct
      241 aatcaatatg accaatattg tcaatactaa ccctttcaat caattcatta gttatctaaa
      301 ctaagttcat taat
//
LOCUS       DAVMEI010000359          305 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.379, whole genome shotgun sequence.
ACCESSION   DAVMEI010000359 DAVMEI010000000
VERSION     DAVMEI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.379"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>305)
                     /locus_tag="VYQ24_002115"
     CDS             complement(<1..>305)
                     /locus_tag="VYQ24_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEV1969169.1"
                     /translation="NLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHR
                     FLESKGIQASISRKGNSPDNSMMESFFGILKSEMFYGYEKSFQSLKQLEQAIIDYID"
BASE COUNT           78 a           61 c           62 g          104 t
ORIGIN      
        1 taatcaatat agtctataat ggcttgttcc aattgcttaa gcgactgaaa cgacttctca
       61 taaccgtaaa acatttccga tttcagaatc ccaaagaagg actccatcat actattgtct
      121 gggctgtttc ccttgcgtga tatggatgct tgaattccct tactctctag gaaccgatga
      181 taagaatcgt gttggtattg ccagccttgg tcactatgga gaatcgtatt ctcgtagtgc
      241 ttctctgtga atgcctgttc caacattgtt tgtacttgtt ctaagttggg tgaagttgaa
      301 agatt
//
LOCUS       DAVMEI010000360          303 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.381, whole genome shotgun sequence.
ACCESSION   DAVMEI010000360 DAVMEI010000000
VERSION     DAVMEI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.381"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..303)
                     /locus_tag="VYQ24_002116"
                     /pseudo
     CDS             complement(1..303)
                     /locus_tag="VYQ24_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           95 a           60 c           48 g          100 t
ORIGIN      
        1 taataaccga tggtgtggtt aatgttggta agagaaactt ctgaaaccaa gcttcaaaaa
       61 agtcgctcgt catcatctct tcgtaagtca ttggagcgat taactcacca tttgttagac
      121 ctgcaaccaa agaaatcctc tgatatcttc ttccagatac tttacctctt cttaactgac
      181 cttttaatga gcgaccatat tctcgataaa aataagtatc gaatcctgtt tcatcaatct
      241 aaacaggtgc taggtgcttt aaactattaa aatttttaag aaataaggct actttttctg
      301 ggt
//
LOCUS       DAVMEI010000361          302 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.382, whole genome shotgun sequence.
ACCESSION   DAVMEI010000361 DAVMEI010000000
VERSION     DAVMEI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.382"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            1..302
                     /locus_tag="VYQ24_002117"
                     /pseudo
     CDS             1..302
                     /locus_tag="VYQ24_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT           91 a           57 c           73 g           81 t
ORIGIN      
        1 tctaaaaatc gaaggacgta tggagtggag tctattcact acgtatcaac agtaaccaac
       61 tgctacaagg cggctgtgga tgcctatctt gaaagtcctg aaaagtttga agctatcaaa
      121 caagacttgg tggacgagat gtggaaggtt gcccaacgtg aactggctac aggattttac
      181 tatggtatac catctgaaaa tgagcagttg tttggtgctc gtcgtaaaat tcctgagtac
      241 aagtttgtcg ctgaagtgat ttcttatgat gatgcgacac aaacagcaac cattcgtcaa
      301 cg
//
LOCUS       DAVMEI010000362          297 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.383, whole genome shotgun sequence.
ACCESSION   DAVMEI010000362 DAVMEI010000000
VERSION     DAVMEI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.383"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>297
                     /locus_tag="VYQ24_002118"
     CDS             <1..>297
                     /locus_tag="VYQ24_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1969170.1"
                     /translation="LKIEGRMKSIHYVSTVTNCYKAAVDAYLESPEKFEAIKQDLVDE
                     MWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVVSYDDATQTATIRQR"
BASE COUNT           91 a           60 c           69 g           77 t
ORIGIN      
        1 tctaaaaatc gaaggacgta tgaagtctat tcactacgta tcaacagtaa ccaactgcta
       61 caaggcggct gtggatgcct atcttgaaag tcctgaaaag tttgaagcta tcaaacaaga
      121 cttagtggac gagatgtgga aggttgccca acgtgaattg gcaacaggtt tctactacgg
      181 tataccatct gaaaatgagc agttgtttgg tgctcgccgt aaaatccctg agtacaagtt
      241 tgtcgctgaa gtggtttctt atgatgatgc gacacaaaca gcaaccattc gtcaacg
//
LOCUS       DAVMEI010000363          289 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.386, whole genome shotgun sequence.
ACCESSION   DAVMEI010000363 DAVMEI010000000
VERSION     DAVMEI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.386"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(1..289)
                     /locus_tag="VYQ24_002119"
                     /pseudo
     CDS             complement(1..289)
                     /locus_tag="VYQ24_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           91 a           58 c           65 g           75 t
ORIGIN      
        1 attttgacgc gacatcggac agctctatcg tagcgaagaa agtgatttcg aatgatagct
       61 tgtgttcttc cctcaagaac agtgatgata ttgagattgt taaaatcttg cgcaatgaag
      121 ctcatctcca tctcccgatt gaaacagtca ctccccggac tgtttcaacg tcccaggaca
      181 taatctcagg aagacgcgaa aaatcatgct caaagtgaga atcattgagc ttgcgaatga
      241 cagttgaagt tgagatggaa agctgatggg caatatcagt catagaaat
//
LOCUS       DAVMEI010000364          283 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.389, whole genome shotgun sequence.
ACCESSION   DAVMEI010000364 DAVMEI010000000
VERSION     DAVMEI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.389"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT           86 a           58 c           41 g           98 t
ORIGIN      
        1 ttttttcaaa atatctaaga catcacttct ttgtttaatt tgataggctt tgatacctga
       61 gctccgactt tatcacttaa aaaataaaaa agacagctaa tctcttgcgc atcaagggat
      121 ttcgccattc tttaatctcg tttaagtgtt gtatatggag ctttgcataa ttttttaaaa
      181 ctaaacaact acattaagca cgaactgtga cgctggttcc atcttcctta taaagattga
      241 taagcccctc tttaagagct cgaaccatgt ctccagcttg aac
//
LOCUS       DAVMEI010000365          280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.390, whole genome shotgun sequence.
ACCESSION   DAVMEI010000365 DAVMEI010000000
VERSION     DAVMEI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.390"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>280
                     /locus_tag="VYQ24_002120"
     CDS             <1..>280
                     /locus_tag="VYQ24_002120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1969171.1"
                     /translation="IVSIATLLFYIDYFQILDNQILFLTIFVVIVLKIADEFVLLSLY
                     ASSINIVHEEEQQKLRSWQQISANCAAIISPIIGATLYGAIPFNLFILA"
BASE COUNT           85 a           42 c           40 g          113 t
ORIGIN      
        1 gatagtaagt atagctactc ttttatttta tattgattat tttcaaattt tagataatca
       61 aattctcttt ttgactatct ttgttgtaat cgttttaaaa attgcagacg aatttgtgct
      121 tttgtctcta tatgcaagta gcatcaacat tgttcatgaa gaagaacaac aaaagttacg
      181 ttcgtggcaa caaatttcag caaattgtgc agcaattata tctcctatta ttggtgcaac
      241 actttatgga gccatccctt ttaatttgtt tattttagca
//
LOCUS       DAVMEI010000366          271 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.392, whole genome shotgun sequence.
ACCESSION   DAVMEI010000366 DAVMEI010000000
VERSION     DAVMEI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.392"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            20..>271
                     /locus_tag="VYQ24_002121"
     CDS             20..>271
                     /locus_tag="VYQ24_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1969172.1"
                     /translation="MRIKEKTNNINGGIKNVSKHYGHSIILKDINFALNKGEIVGLAG
                     RNGVGKSTLMKILVQNNQPTSGNIISSDNVGYLIEEPKLF"
BASE COUNT          108 a           29 c           53 g           81 t
ORIGIN      
        1 gcgcaacttt ttaaaattaa tgagaatcaa agagaaaacc aataatatta atggaggaat
       61 aaaaaatgta agtaagcatt atggtcattc aatcattctc aaagatataa attttgcact
      121 taacaagggt gaaattgttg gtctagcagg gagaaatgga gttggtaaga gtacgttgat
      181 gaaaattctt gttcagaata atcaaccgac ttcaggtaat attataagca gtgataatgt
      241 tgggtattta atcgaagaac caaaattatt t
//
LOCUS       DAVMEI010000367          263 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.395, whole genome shotgun sequence.
ACCESSION   DAVMEI010000367 DAVMEI010000000
VERSION     DAVMEI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.395"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
BASE COUNT           92 a           31 c           53 g           87 t
ORIGIN      
        1 tgtctttgat ttatagtagt gctaaaaagg acttccttta ttggaatgtc cttattttta
       61 ttatggaatt gccaaatgac gttaaggttc agttctatga acttagaaag aaagtacaaa
      121 gctttaatca gctttcaaaa agatttggta tggatgtttc tggctaaggt atgtgacaag
      181 gtgaattgat cgttattgaa cagagagcgt taaaaaaggg aagaatcgtt actattccac
      241 tgaattaaaa caagaaataa atc
//
LOCUS       DAVMEI010000368          262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.396, whole genome shotgun sequence.
ACCESSION   DAVMEI010000368 DAVMEI010000000
VERSION     DAVMEI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.396"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..88
                     /locus_tag="VYQ24_002122"
     CDS             <1..88
                     /locus_tag="VYQ24_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV1969173.1"
                     /translation="NKDKTKEKGKRKVKEKVKPNKKDMTREI"
BASE COUNT          144 a           34 c           43 g           41 t
ORIGIN      
        1 aaacaaagat aagacaaaag aaaaaggaaa aaggaaagta aaagaaaaag taaaaccaaa
       61 caaaaaagat atgaccagag aaatctaaag ggagtaacga aaagttacac ccttaaataa
      121 ccataataac aagacttccg aaaaacagaa gtccagaatt ccgaaaatcg gaaatcaaga
      181 atttcgatta tcgaaaatca agaaaggagc agatatggca acaaacaaaa gatactattg
      241 gataaaatta aaagaagaat tt
//
LOCUS       DAVMEI010000369          262 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.397, whole genome shotgun sequence.
ACCESSION   DAVMEI010000369 DAVMEI010000000
VERSION     DAVMEI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.397"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>262
                     /locus_tag="VYQ24_002123"
     CDS             <1..>262
                     /locus_tag="VYQ24_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV1969174.1"
                     /translation="KYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAK
                     ISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNK"
BASE COUNT          111 a           46 c           34 g           71 t
ORIGIN      
        1 aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg
       61 aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg
      121 aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac
      181 ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta
      241 cagtttcaat tccctaacaa ac
//
LOCUS       DAVMEI010000370          251 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.398, whole genome shotgun sequence.
ACCESSION   DAVMEI010000370 DAVMEI010000000
VERSION     DAVMEI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.398"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            48..>251
                     /locus_tag="VYQ24_002124"
     CDS             48..>251
                     /locus_tag="VYQ24_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HEV1969175.1"
                     /translation="MTEEKFQKIIQSKDTETLAFILESTPYHLSIDILEDPSQTEISL
                     MTKLVNDYRWAYAESPSDIIVTLF"
BASE COUNT           95 a           38 c           38 g           80 t
ORIGIN      
        1 ttttttcaaa aataaatacg acgatttcgg taaaagaaaa cgattttatt acagaagaaa
       61 aatttcaaaa aattatacaa tccaaagata cggagacatt ggcatttatc ttagaatcaa
      121 ctccctatca tttatcgatt gacatcttag aagatcctag tcagacagag atttcgctaa
      181 tgacaaaatt agtcaatgat tatagatggg cctatgctga aagtccgtct gatataattg
      241 tgactttatt t
//
LOCUS       DAVMEI010000371          240 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.403, whole genome shotgun sequence.
ACCESSION   DAVMEI010000371 DAVMEI010000000
VERSION     DAVMEI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.403"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>240
                     /locus_tag="VYQ24_002125"
     CDS             <1..>240
                     /locus_tag="VYQ24_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEV1969176.1"
                     /translation="YNLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYH
                     RFLESKGIQASMSRKGNSQDNGMMESFFGILKFMA"
BASE COUNT           80 a           47 c           49 g           64 t
ORIGIN      
        1 ctataatctt tcaacttcac ccaacttaga acaagtgaag agtatgttag aacaggcctt
       61 tacagagaaa tactatgaga atacgattct ccatagtgac caaggctggc aatatcaaca
      121 cgattcttat catcggttct tagagagtaa gggaattcaa gcatccatgt cacgcaaggg
      181 aaacagccaa gacaacggta tgatggagtc cttctttggg attctgaaat ttatggctat
//
LOCUS       DAVMEI010000372          237 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.404, whole genome shotgun sequence.
ACCESSION   DAVMEI010000372 DAVMEI010000000
VERSION     DAVMEI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.404"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            3..152
                     /gene="blpO"
                     /locus_tag="VYQ24_002126"
     CDS             3..152
                     /gene="blpO"
                     /locus_tag="VYQ24_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1969177.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
BASE COUNT           66 a           29 c           70 g           72 t
ORIGIN      
        1 atatgaatac aaaaatgatg tcacaatttt ctgttatgga taatgaaatg cttgcttgcg
       61 ttgaaggtgg agatattgat tggggaagaa aaattagttg tgcagcaggg gttgcatatg
      121 gcgcaattga tgggtgtgca acaacggttt gacatttcta ttgggaccat ttgctatagg
      181 aataggtgta actggtgctg caggtggagc tatacttgga ggtgtagcct atgcagc
//
LOCUS       DAVMEI010000373          235 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.405, whole genome shotgun sequence.
ACCESSION   DAVMEI010000373 DAVMEI010000000
VERSION     DAVMEI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.405"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            <1..>235
                     /locus_tag="VYQ24_002127"
     CDS             <1..>235
                     /locus_tag="VYQ24_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1969178.1"
                     /translation="TVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLAT
                     VKYYVEHPNERPHSDNGFGNASDHVQRNKNGQAD"
BASE COUNT           77 a           45 c           49 g           64 t
ORIGIN      
        1 actgtagaag tcaaaaacgg tagtttaatc atacctcatt atgaccatta ccataacatc
       61 aaatttgagt ggtttgacga aggcctttat gaggcaccta aggggtatac tcttgaggat
      121 cttttggcga ctgtcaagta ctatgtcgaa catccaaacg aacgtccgca ttcagataat
      181 ggttttggta acgctagcga ccatgttcaa agaaacaaaa atggtcaagc tgata
//
LOCUS       DAVMEI010000374          232 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.406, whole genome shotgun sequence.
ACCESSION   DAVMEI010000374 DAVMEI010000000
VERSION     DAVMEI010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.406"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            complement(<1..>232)
                     /locus_tag="VYQ24_002128"
     CDS             complement(<1..>232)
                     /locus_tag="VYQ24_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1969179.1"
                     /translation="IRKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNI
                     ITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMD"
BASE COUNT           76 a           46 c           44 g           66 t
ORIGIN      
        1 tatccatcgt aatgattttc acttgacaac gaacggctct atcgtagcga agaaagtgat
       61 ttcggatgac agcttgtgtt ctgccttcaa gaacagtgat aatattaaga ttatcaaaat
      121 cttgcgcaat gaaactcatc tttcccttag tgaaggcata ctcatcccaa gacataatct
      181 ttggaagccg agaaaaatca tgctcaaagt gaaagtcatt gagcttgcga at
//
LOCUS       DAVMEI010000375          227 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.407, whole genome shotgun sequence.
ACCESSION   DAVMEI010000375 DAVMEI010000000
VERSION     DAVMEI010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.407"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            30..>227
                     /locus_tag="VYQ24_002129"
     CDS             30..>227
                     /locus_tag="VYQ24_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1969180.1"
                     /translation="MNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTNKKDAHGIALK
                     LLSDPNREQFQHDNRQVELKIL"
BASE COUNT           81 a           53 c           32 g           61 t
ORIGIN      
        1 aagatactgg acattatgcc tttaatctac tgaacttcct gcatgaacaa ggatataaag
       61 tttacaccta cactcctttg ctcatcaagg aatttgccaa atcattatca cttcgcaaga
      121 ctaaaacgaa taagaaagac gctcatggca tcgctcttaa acttctttct gaccccaatc
      181 gtgaacaatt tcaacacgat aatcggcaag tagaactaaa aatactg
//
LOCUS       DAVMEI010000376          202 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.409, whole genome shotgun sequence.
ACCESSION   DAVMEI010000376 DAVMEI010000000
VERSION     DAVMEI010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.409"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            45..128
                     /locus_tag="VYQ24_002130"
     CDS             45..128
                     /locus_tag="VYQ24_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEV1969181.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT           84 a           27 c           30 g           61 t
ORIGIN      
        1 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
       61 tgcgtaatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag
      121 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
      181 taactaaaat tacaaacaaa tc
//
LOCUS       DAVMEI010000377          201 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.denovo.410, whole genome shotgun sequence.
ACCESSION   DAVMEI010000377 DAVMEI010000000
VERSION     DAVMEI010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.denovo.410"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            76..149
                     /locus_tag="VYQ24_002131"
     tRNA            76..149
                     /locus_tag="VYQ24_002131"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:110..112,aa:Met,seq:cat)
BASE COUNT           46 a           48 c           59 g           48 t
ORIGIN      
        1 gggaacggtc ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc
       61 ctccttttat attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa
      121 ggtcgtaggt tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat
      181 ggattgaagc tccatgtgtc g
//
LOCUS       DAVMEI010000378         2902 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.guided.171, whole genome shotgun sequence.
ACCESSION   DAVMEI010000378 DAVMEI010000000
VERSION     DAVMEI010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.guided.171"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            30..431
                     /locus_tag="VYQ24_002132"
                     /pseudo
     CDS             30..431
                     /locus_tag="VYQ24_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
     gene            428..970
                     /gene="sat4"
                     /locus_tag="VYQ24_002133"
     CDS             428..970
                     /gene="sat4"
                     /locus_tag="VYQ24_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEV1969182.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1063..1857
                     /locus_tag="VYQ24_002134"
                     /note="aph(3')-IIIa"
     CDS             1063..1857
                     /locus_tag="VYQ24_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEV1969183.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            2133..2706
                     /locus_tag="VYQ24_002135"
                     /pseudo
     CDS             2133..2706
                     /locus_tag="VYQ24_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
BASE COUNT          978 a          461 c          674 g          789 t
ORIGIN      
        1 tctattttta atagttatct attatttaac gggaggaaat tttatttgct attgatcatt
       61 ttaatcagat tgttcgccat gagctgctga gaatgatatc atggaaggtc ggcatcgaaa
      121 caggctttaa attaagtgta ggcaagaact ataagtttat tgaaaggtat atatccgagg
      181 atttgtggga gaaacttttg tccacctacc ggatggattc ctatgaaaac atatgggaag
      241 cattatttct atgccatcaa ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc
      301 attatgccta tccggagtat gataggaata taacaaaata taccagggac atgtataaaa
      361 aatacactgg taaaaccggc tgcctggata gcacatatgc cgctgatata gaagagaggc
      421 gggaacagtg attacagaaa tgaaagcagg gcacctgaaa gatatcgata aacccagcga
      481 accatttgag gtgataggta agattatacc gaggtatgaa aacgagaatt ggacctttac
      541 agaattactc tatgaagcgc catatttaaa aagctaccaa gacgaagagg atgaagagga
      601 tgaggaggca gattgccttg aatatattga caatactgat aagataatat atctttacta
      661 ccaagacgat aaatgcgtcg gaaaagttaa actgcgaaaa aattggaacc ggtacgctta
      721 tatagaagat atcgccgtat gtaaggattt cagggggcaa ggcataggca gcgcgcttat
      781 caatatatct atagaatggg caaagcataa aaacttgcat ggactaatgc ttgaaaccca
      841 ggacaataac cttatagctt gtaaattcta tcataattgt ggtttcaaaa tcggctccgt
      901 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
      961 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
     1021 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
     1081 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     1141 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     1201 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     1261 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     1321 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     1381 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     1441 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     1501 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
     1561 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     1621 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     1681 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     1741 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     1801 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
     1861 ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg
     1921 aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga
     1981 tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt
     2041 ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac
     2101 tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta
     2161 ttcttattca gctgcaatca aaaaaaaata attcctgcca aagaaattgc acagcgattt
     2221 aatataagct taaggacagt ttacagagat atccggacac ttgaagaagc cggaatacca
     2281 attggatctg aggccggaaa aggatatttt cttgtagagg gcttcctact tccgccggta
     2341 atgtttacag cggcggaagt gggtgcattg attacagcag ggaaattttt aaattgccat
     2401 ggagatgaat catttataaa ggattttgat tcagctatgt ataaaatcaa atctattttg
     2461 aagcatggtg aaaaaaacta tgcacaggag ttggagaaca gtattaacgt gtacagcaca
     2521 tctggacaga aaaatacatt ggcggataac gttattgcag caattcagac tgcaatctgc
     2581 aataaaaggg taatatcaat tcaatatcct gcatcaggag ggcaagaacc ggaaagcaga
     2641 atgatagagc ctatatcact ggggttttat gaacagaact ggtacttaat cggttttgca
     2701 ggctaagaaa tgaatatagg aattttcgtg tggatcggat taaaagcatt tgcatagaag
     2761 aagagctctg tcaaagccat gatggctctt tggaacaaat attaaaacaa atgctttcat
     2821 ataaaaaact atatgcccta caatgccgtt gtaaaaattg gggaaaaggg atatattgat
     2881 tttatatctt tctcaaaata aa
//
LOCUS       DAVMEI010000379         2303 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.guided.189, whole genome shotgun sequence.
ACCESSION   DAVMEI010000379 DAVMEI010000000
VERSION     DAVMEI010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.guided.189"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            232..348
                     /locus_tag="VYQ24_002136"
     CDS             232..348
                     /locus_tag="VYQ24_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEV1969184.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            364..2283
                     /gene="tet(M)"
                     /locus_tag="VYQ24_002137"
     CDS             364..2283
                     /gene="tet(M)"
                     /locus_tag="VYQ24_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEV1969185.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLRYYVDSATHEIILSFLG
                     KVQMEVTCALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
BASE COUNT          776 a          356 c          460 g          711 t
ORIGIN      
        1 atgattacag atactttgta atcatgtact ctttttgata aaaaattgga gattccttta
       61 caaatatgct cttatgtgct attatttaag tgactattta aaaggagtta ataaatatgc
      121 ggcaaggtat tcttaaataa actgtcaatt tgatagcgga aacaaataat tagatgtcct
      181 tttttaggag ggcttagttt tttgtaccca gtttaagaat acctttatca tgtgattcta
      241 aagtatccag agaatatctg tatgctttgt atacctatgg ttatgcataa aaatcccagt
      301 gataaaagta tttatcactg ggatttttat gcccttttgg gtttttgaat ggaggaaaat
      361 cacatgaaaa ttattaatat tggagtttta gctcatgttg atgcaggaaa aactacctta
      421 acagaaagct tattatataa cagtggagcg attacagaat taggaagcgt ggacaaaggt
      481 acaacgagga cggataatac gcttttagaa cgtcagagag gaattacaat tcagacagga
      541 ataacctctt ttcagtggga aaatacgaag gtgaacatca tagacacgcc aggacatatg
      601 gatttcttag cagaagtata tcgttcatta tcagttttag atggggcaat tctactgatt
      661 tctgcaaaag atggcgtaca agcacaaact cgtatattat ttcatgcact taggaaaatg
      721 gggattccca caatcttttt tatcaataag attgaccaaa atggaattga tttatcaacg
      781 gtttatcagg atattaaaga gaaactttct gccgaaattg taatcaaaca gaaggtagaa
      841 ctgtatccta atatgtgtgt gacgaacttt accgaatctg aacaatggga tacggtaata
      901 gagggaaacg atgacctttt agagaaatat atgtccggta aatcattaga agcattggaa
      961 ctcgaacaag aggaaagcat aagatttcag aattgttctc tgttccctct ttatcatgga
     1021 agtgcaaaaa gtaatatagg gattgataac cttatagaag ttattactaa taaattttat
     1081 tcatcaacac atcgaggtcc gtctgaactt tgcggaaatg ttttcaaaat tgaatataca
     1141 aaaaaaagac aacgtcttgc atatatacgc ctttatagtg gagtactaca tttacgagat
     1201 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
     1261 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
     1321 tttttgaagt taaatagtgt tcttggagat acaaagctat tgccacagag agagagaatt
     1381 gaaaatcccc tccctctgct gcaaacgact gttgaaccga gcaaacctca acaaagggaa
     1441 atgttacttg atgcactttt agaaatctcc gacagtgacc cgcttctgcg atattatgtg
     1501 gattctgcga cacatgaaat catactttct ttcttaggga aagtacaaat ggaagtgact
     1561 tgtgctctgc tgcaagaaaa gtatcatgtg gagatagaaa taaaagagcc tacagtcatt
     1621 tatatggaaa gaccgttaaa aaaagcagag tataccattc acatcgaagt tccaccgaat
     1681 cctttctggg cttccattgg tctatctgta gcaccgcttc cattagggag cggagtacag
     1741 tatgagagct cggtttctct tggatactta aatcaatcgt ttcaaaatgc agttatggag
     1801 gggatacgct atggctgtga acaaggattg tatggttgga atgtgacgga ctgtaaaatc
     1861 tgttttaagt atggcttata ctatagccct gttagtaccc cagcagattt tcggatgctt
     1921 gctcctattg tattggaaca agtcttaaaa aaagctggaa cagaattgtt agagccatat
     1981 cttagtttta aaatttatgc gccacaggaa tatctttcac gagcatacaa cgatgctcct
     2041 aaatattgtg cgaacatcgt agacactcaa ttgaaaaata atgaggtcat tcttagtgga
     2101 gaaatccctg ctcggtgtat tcaagaatat cgtagtgatt taactttctt tacaaatgga
     2161 cgtagtgttt gtttaacaga gttaaaaggg taccatgtta ctaccggtga acctgtttgc
     2221 cagccccgtc gtccaaatag tcggatagat aaagtacgat atatgttcaa taaaataact
     2281 tagtgtattt tatgttgtta tat
//
LOCUS       DAVMEI010000380         2200 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.guided.195, whole genome shotgun sequence.
ACCESSION   DAVMEI010000380 DAVMEI010000000
VERSION     DAVMEI010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.guided.195"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            7..744
                     /gene="erm(B)"
                     /locus_tag="VYQ24_002138"
     CDS             7..744
                     /gene="erm(B)"
                     /locus_tag="VYQ24_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV1969186.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSALIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKYA
                     KVNDLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1001..>2200)
                     /locus_tag="VYQ24_002139"
     CDS             complement(1001..>2200)
                     /locus_tag="VYQ24_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1969187.1"
                     /translation="NIKIISVEEAEHNNDSVKEYITLITATLSYPINRCRNCGFPTVN
                     KDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITNLTKENQTLSSDLKNQI
                     MLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLPKHLCFDEFRSIKSVMS
                     FICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSVVIDLNAQYQSFIYRLF
                     PNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMKSHWKLFHKKAEDLHPE
                     EVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKALNERNSELLESTILDYQ
                     KTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKTLKRTCYGFANQKFFFL
                     RIDCIFS"
BASE COUNT          733 a          368 c          412 g          687 t
ORIGIN      
        1 gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc
       61 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca
      121 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
      181 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact
      241 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt
      301 gggaatattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccgt
      361 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac
      421 cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca
      481 gctgaatgct ttcatcctaa accaaaagta aacagtgcct taataaaact tacccgccat
      541 accacagatg ttccagataa atattggaaa ctatatacgt actttgtttc aaaatgggtc
      601 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaatacgcc
      661 aaagtaaacg atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat
      721 ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg gaaagttaca
      781 cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt ccaagaagct
      841 aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa ttcccactaa
      901 gcgctcggga ccccttgtag gaaaatgtcc taagtgggat atctgtcaac tggtatggtt
      961 gactaaaaat acttcctacg aaaatgtagg gggtattttt ttacgaaaaa atacaatcga
     1021 ttcttaaaaa gaaaaatttt tgattggcaa aaccataaca agttcgtttt agggttttga
     1081 ttttgcgatt gatgccttct aaaggaccat tagagtattc aaatttagcg ctatttaaga
     1141 catattttct gttttgacga agggtttgaa tagcagtatc catttctgta ttggtttttt
     1201 ggtagtctaa gatggttgac tctagtaatt cactattgcg ctcgtttagg gctttcgtga
     1261 tatcttggta agtttggtat acttcagcga acttggaaaa tttactagta atgagatcaa
     1321 cagcattttg gcgagtcata tattgtttaa cgccgcgaag aaaaactact tcttcagggt
     1381 gaagatcttc agctttttta tggaatagct tccaatgtga cttcataatt ttatattctt
     1441 ggctctgttt atcaagttgc tttaggatag agatacgaca attgtccaaa gcgcgaccag
     1501 ctaattgtac aaggtggaag cgatcaataa tgatattggc attagggaaa aggcgataga
     1561 taaaactttg atattgagca tttaaatcaa ttacaactga ttgaacgcat tcgcgttcgg
     1621 cttttgaata acgactttca aaataatcaa caatggtagg tgatagacga tcctgtaact
     1681 ttgtgacaat ttggtgggtt tcagcgtcac aacagataaa ggacatcaca gacttaattg
     1741 aacgaaactc gtcaaaacat agatgcttag gcaacttagc cacacgatag tgtggttcca
     1801 tgcgctctaa gattgttcga cgaacactgc taggagagca gtgacacatt tcagcaataa
     1861 gctgaccaga taagccttta cgagctaaaa gcatgatttg atttttgaga tcactggata
     1921 aggtttgatt ttctttggtt aaattagtaa tagcaccaaa agtagtatgg catgatttac
     1981 atttatagcg ttgtttacga agctctagtt catatcttct cccatttaaa cttgccagtc
     2041 gtacatgagt tttgcgaaag ccatccttat taactgtggg aaagccacag ttacgacaac
     2101 gattaatcgg ataagaaaga gtagctgtta ttagcgttat atactcttta acagaatcgt
     2161 tgttgtgttc agcttcttca acagaaataa ttttaatatt
//
LOCUS       DAVMEI010000381         2200 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae isolate GC089790_E24
            SAMN29867844-rid17926453.guided.196, whole genome shotgun sequence.
ACCESSION   DAVMEI010000381 DAVMEI010000000
VERSION     DAVMEI010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29867844
            Sequence Read Archive: SRR20340580
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001720078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001720078.1
            Genome Coverage       :: 335x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/05/2023 19:57:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,105
            Genes (coding)                    :: 1,990
            CDSs (with protein)               :: 1,990
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 28
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 59 of 115
            Pseudo Genes (incomplete)         :: 45 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 20 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29867844-rid17926453.guided.196"
                     /isolate="GC089790_E24"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Belgium"
                     /lat_lon="50.879611 N 4.670850 E"
                     /collection_date="2018-03-09"
                     /collected_by="NRC_UZ_LEUVEN"
     gene            7..744
                     /gene="erm(B)"
                     /locus_tag="VYQ24_002140"
     CDS             7..744
                     /gene="erm(B)"
                     /locus_tag="VYQ24_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEV1969188.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIKQLLKLPAE
                     CFHPKPKVNSALIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKYA
                     KVNDLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1001..>2200)
                     /locus_tag="VYQ24_002141"
     CDS             complement(1001..>2200)
                     /locus_tag="VYQ24_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1969189.1"
                     /translation="NIKIISVEEAEHNNDSVKEYITLITATLSYPINRCRNCGFPTVN
                     KDGFRKTHVRLASLNGRRYELELRKQRYKCKSCHTTFGAITNLTKENQTLSSDLKNQI
                     MLLARKGLSGQLIAEMCHCSPSSVRRTILERMEPHYRVAKLPKHLCFDEFRSIKSVMS
                     FICCDAETHQIVTKLQDRLSPTIVDYFESRYSKAERECVQSVVIDLNAQYQSFIYRLF
                     PNANIIIDRFHLVQLAGRALDNCRISILKQLDKQSQEYKIMKSHWKLFHKKAEDLHPE
                     EVVFLRGVKQYMTRQNAVDLITSKFSKFAEVYQTYQDITKALNERNSELLESTILDYQ
                     KTNTEMDTAIQTLRQNRKYVLNSAKFEYSNGPLEGINRKIKTLKRTCYGFANQKFFFL
                     RIDCIFS"
BASE COUNT          732 a          367 c          414 g          687 t
ORIGIN      
        1 gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc
       61 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca
      121 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa
      181 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact
      241 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt
      301 gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccgt
      361 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac
      421 cgaacactag ggttgctctt gcacactcaa gtctcgatta agcaattgct taagctgcca
      481 gctgaatgct ttcatcctaa accaaaagta aacagtgcct taataaaact tacccgccat
      541 accacagatg ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc
      601 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaatacgcc
      661 aaagtaaacg atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat
      721 ctattattta acgggaggaa ataattctat gagtcgcttt tttaaatttg gaaagttaca
      781 cgttactaaa gggaatggag ataaattatt agatatacta ctgacagctt ccaagaagct
      841 aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa ttcccactaa
      901 gcgctcggga ccccttgtag gaaaatgtcc taagtgggat atctgtcaac tggtatggtt
      961 gactaaaaat acttcctacg aaaatgtagg gggtattttt ttacgaaaaa atacaatcga
     1021 ttcttaaaaa gaaaaatttt tgattggcaa aaccataaca agttcgtttt agggttttga
     1081 ttttgcgatt gatgccttct aaaggaccat tagagtattc aaatttagcg ctatttaaga
     1141 catattttct gttttgacga agggtttgaa tagcagtatc catttctgta ttggtttttt
     1201 ggtagtctaa gatggttgac tctagtaatt cactattgcg ctcgtttagg gctttcgtga
     1261 tatcttggta agtttggtat acttcagcga acttggaaaa tttactagta atgagatcaa
     1321 cagcattttg gcgagtcata tattgtttaa cgccgcgaag aaaaactact tcttcagggt
     1381 gaagatcttc agctttttta tggaatagct tccaatgtga cttcataatt ttatattctt
     1441 ggctctgttt atcaagttgc tttaggatag agatacgaca attgtccaaa gcgcgaccag
     1501 ctaattgtac aaggtggaag cgatcaataa tgatattggc attagggaaa aggcgataga
     1561 taaaactttg atattgagca tttaaatcaa ttacaactga ttgaacgcat tcgcgttcgg
     1621 cttttgaata acgactttca aaataatcaa caatggtagg tgatagacga tcctgtaact
     1681 ttgtgacaat ttggtgggtt tcagcgtcac aacagataaa ggacatcaca gacttaattg
     1741 aacgaaactc gtcaaaacat agatgcttag gcaacttagc cacacgatag tgtggttcca
     1801 tgcgctctaa gattgttcga cgaacactgc taggagagca gtgacacatt tcagcaataa
     1861 gctgaccaga taagccttta cgagctaaaa gcatgatttg atttttgaga tcactggata
     1921 aggtttgatt ttctttggtt aaattagtaa tagcaccaaa agtagtatgg catgatttac
     1981 atttatagcg ttgtttacga agctctagtt catatcttct cccatttaaa cttgccagtc
     2041 gtacatgagt tttgcgaaag ccatccttat taactgtggg aaagccacag ttacgacaac
     2101 gattaatcgg ataagaaaga gtagctgtta ttagcgttat atactcttta acagaatcgt
     2161 tgttgtgttc agcttcttca acagaaataa ttttaatatt
//
